LOCUS       DAFGFZ010000001        88291 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000001 DAFGFZ010000000
VERSION     DAFGFZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.001"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            2..580
                     /locus_tag="LR726_000001"
     CDS             2..580
                     /locus_tag="LR726_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215120.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(820..903)
                     /locus_tag="LR726_000002"
     tRNA            complement(820..903)
                     /locus_tag="LR726_000002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(867..869),aa:Leu,seq:gag)
     gene            958..1137
                     /locus_tag="LR726_000003"
     CDS             958..1137
                     /locus_tag="LR726_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215121.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1286..2503
                     /locus_tag="LR726_000004"
     CDS             1286..2503
                     /locus_tag="LR726_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL6215122.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2687..3523
                     /locus_tag="LR726_000005"
     CDS             2687..3523
                     /locus_tag="LR726_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6215123.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3599..4747
                     /gene="nagA"
                     /locus_tag="LR726_000006"
     CDS             3599..4747
                     /gene="nagA"
                     /locus_tag="LR726_000006"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL6215124.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4968..6026
                     /locus_tag="LR726_000007"
     CDS             4968..6026
                     /locus_tag="LR726_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL6215125.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6030..7064
                     /locus_tag="LR726_000008"
     CDS             6030..7064
                     /locus_tag="LR726_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL6215126.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7166..7882)
                     /locus_tag="LR726_000009"
     CDS             complement(7166..7882)
                     /locus_tag="LR726_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL6215127.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7851..8636)
                     /locus_tag="LR726_000010"
     CDS             complement(7851..8636)
                     /locus_tag="LR726_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL6215128.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8627..9376)
                     /locus_tag="LR726_000011"
     CDS             complement(8627..9376)
                     /locus_tag="LR726_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL6215129.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9674..10198
                     /locus_tag="LR726_000012"
     CDS             9674..10198
                     /locus_tag="LR726_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL6215130.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10214..11713
                     /locus_tag="LR726_000013"
     CDS             10214..11713
                     /locus_tag="LR726_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL6215131.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11801..12403)
                     /locus_tag="LR726_000014"
     CDS             complement(11801..12403)
                     /locus_tag="LR726_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL6215132.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12507..13463
                     /locus_tag="LR726_000015"
     CDS             12507..13463
                     /locus_tag="LR726_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL6215133.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13768..14724)
                     /locus_tag="LR726_000016"
     CDS             complement(13768..14724)
                     /locus_tag="LR726_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL6215134.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14898..15458
                     /locus_tag="LR726_000017"
     CDS             14898..15458
                     /locus_tag="LR726_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215135.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15451..16275
                     /locus_tag="LR726_000018"
     CDS             15451..16275
                     /locus_tag="LR726_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL6215136.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16285..16902
                     /locus_tag="LR726_000019"
     CDS             16285..16902
                     /locus_tag="LR726_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL6215137.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16920..17603
                     /locus_tag="LR726_000020"
     CDS             16920..17603
                     /locus_tag="LR726_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL6215138.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18286..19569
                     /locus_tag="LR726_000021"
     CDS             18286..19569
                     /locus_tag="LR726_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6215139.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19585..20472
                     /locus_tag="LR726_000022"
     CDS             19585..20472
                     /locus_tag="LR726_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6215140.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20489..21325
                     /locus_tag="LR726_000023"
     CDS             20489..21325
                     /locus_tag="LR726_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6215141.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21328..21951
                     /locus_tag="LR726_000024"
     CDS             21328..21951
                     /locus_tag="LR726_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215142.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21948..23240
                     /locus_tag="LR726_000025"
     CDS             21948..23240
                     /locus_tag="LR726_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL6215143.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23599..23696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23812..24393
                     /locus_tag="LR726_000026"
     CDS             23812..24393
                     /locus_tag="LR726_000026"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL6215144.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24396..25703
                     /locus_tag="LR726_000027"
     CDS             24396..25703
                     /locus_tag="LR726_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL6215145.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26119..26607
                     /gene="purE"
                     /locus_tag="LR726_000028"
     CDS             26119..26607
                     /gene="purE"
                     /locus_tag="LR726_000028"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL6215146.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26600..27724
                     /gene="purK"
                     /locus_tag="LR726_000029"
     CDS             26600..27724
                     /gene="purK"
                     /locus_tag="LR726_000029"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL6215147.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27737..29032
                     /gene="purB"
                     /locus_tag="LR726_000030"
     CDS             27737..29032
                     /gene="purB"
                     /locus_tag="LR726_000030"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL6215148.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29212..29589)
                     /locus_tag="LR726_000031"
     CDS             complement(29212..29589)
                     /locus_tag="LR726_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215149.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29845..30573
                     /locus_tag="LR726_000032"
     CDS             29845..30573
                     /locus_tag="LR726_000032"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL6215150.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30573..30827
                     /gene="purS"
                     /locus_tag="LR726_000033"
     CDS             30573..30827
                     /gene="purS"
                     /locus_tag="LR726_000033"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL6215151.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30829..31500
                     /gene="purQ"
                     /locus_tag="LR726_000034"
     CDS             30829..31500
                     /gene="purQ"
                     /locus_tag="LR726_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL6215152.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31501..33723
                     /gene="purL"
                     /locus_tag="LR726_000035"
     CDS             31501..33723
                     /gene="purL"
                     /locus_tag="LR726_000035"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL6215153.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYLPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33708..35147
                     /locus_tag="LR726_000036"
     CDS             33708..35147
                     /locus_tag="LR726_000036"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL6215154.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35170..36222
                     /locus_tag="LR726_000037"
     CDS             35170..36222
                     /locus_tag="LR726_000037"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL6215155.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36219..36797
                     /gene="purN"
                     /locus_tag="LR726_000038"
     CDS             36219..36797
                     /gene="purN"
                     /locus_tag="LR726_000038"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL6215156.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36794..38329
                     /gene="purH"
                     /locus_tag="LR726_000039"
     CDS             36794..38329
                     /gene="purH"
                     /locus_tag="LR726_000039"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL6215157.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38345..39619
                     /gene="purD"
                     /locus_tag="LR726_000040"
     CDS             38345..39619
                     /gene="purD"
                     /locus_tag="LR726_000040"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL6215158.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39741..40655
                     /locus_tag="LR726_000041"
     CDS             39741..40655
                     /locus_tag="LR726_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL6215159.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40788..42353)
                     /locus_tag="LR726_000042"
     CDS             complement(40788..42353)
                     /locus_tag="LR726_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL6215160.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42346..43797)
                     /locus_tag="LR726_000043"
     CDS             complement(42346..43797)
                     /locus_tag="LR726_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL6215161.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43811..44827)
                     /locus_tag="LR726_000044"
     CDS             complement(43811..44827)
                     /locus_tag="LR726_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6215162.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45047..46135
                     /locus_tag="LR726_000045"
     CDS             45047..46135
                     /locus_tag="LR726_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL6215163.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46189..47715
                     /locus_tag="LR726_000046"
     CDS             46189..47715
                     /locus_tag="LR726_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL6215164.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47717..48922
                     /locus_tag="LR726_000047"
     CDS             47717..48922
                     /locus_tag="LR726_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6215165.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49179..50156
                     /gene="guaC"
                     /locus_tag="LR726_000048"
     CDS             49179..50156
                     /gene="guaC"
                     /locus_tag="LR726_000048"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL6215166.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50420..51844
                     /locus_tag="LR726_000049"
     CDS             50420..51844
                     /locus_tag="LR726_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL6215167.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52305..53414
                     /gene="pdhA"
                     /locus_tag="LR726_000050"
     CDS             52305..53414
                     /gene="pdhA"
                     /locus_tag="LR726_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL6215168.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53417..54394
                     /locus_tag="LR726_000051"
     CDS             53417..54394
                     /locus_tag="LR726_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL6215169.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54419..56062
                     /locus_tag="LR726_000052"
     CDS             54419..56062
                     /locus_tag="LR726_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL6215170.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56070..57476
                     /gene="lpdA"
                     /locus_tag="LR726_000053"
     CDS             56070..57476
                     /gene="lpdA"
                     /locus_tag="LR726_000053"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL6215171.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57638..57913
                     /locus_tag="LR726_000054"
     CDS             57638..57913
                     /locus_tag="LR726_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL6215172.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57919..58686
                     /locus_tag="LR726_000055"
     CDS             57919..58686
                     /locus_tag="LR726_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL6215173.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58854..60683
                     /gene="typA"
                     /locus_tag="LR726_000056"
     CDS             58854..60683
                     /gene="typA"
                     /locus_tag="LR726_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL6215174.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60944..62716
                     /gene="recQ"
                     /locus_tag="LR726_000057"
     CDS             60944..62716
                     /gene="recQ"
                     /locus_tag="LR726_000057"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL6215175.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62830..63132
                     /locus_tag="LR726_000058"
     CDS             62830..63132
                     /locus_tag="LR726_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL6215176.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63135..64298
                     /locus_tag="LR726_000059"
     CDS             63135..64298
                     /locus_tag="LR726_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL6215177.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64324..67752
                     /locus_tag="LR726_000060"
     CDS             64324..67752
                     /locus_tag="LR726_000060"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL6215178.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67773..68897
                     /locus_tag="LR726_000061"
     CDS             67773..68897
                     /locus_tag="LR726_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215179.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68887..69324
                     /locus_tag="LR726_000062"
     CDS             68887..69324
                     /locus_tag="LR726_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL6215180.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69308..69646
                     /locus_tag="LR726_000063"
     CDS             69308..69646
                     /locus_tag="LR726_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL6215181.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69765..70325
                     /gene="rsmD"
                     /locus_tag="LR726_000064"
     CDS             69765..70325
                     /gene="rsmD"
                     /locus_tag="LR726_000064"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL6215182.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70322..70813
                     /gene="coaD"
                     /locus_tag="LR726_000065"
     CDS             70322..70813
                     /gene="coaD"
                     /locus_tag="LR726_000065"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL6215183.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70803..71846
                     /locus_tag="LR726_000066"
     CDS             70803..71846
                     /locus_tag="LR726_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL6215184.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72005..72733
                     /locus_tag="LR726_000067"
     CDS             72005..72733
                     /locus_tag="LR726_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL6215185.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72849..73094
                     /locus_tag="LR726_000068"
     CDS             72849..73094
                     /locus_tag="LR726_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL6215186.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73091..73309
                     /locus_tag="LR726_000069"
     CDS             73091..73309
                     /locus_tag="LR726_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL6215187.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73445..73984
                     /locus_tag="LR726_000070"
     CDS             73445..73984
                     /locus_tag="LR726_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL6215188.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73977..76250
                     /locus_tag="LR726_000071"
     CDS             73977..76250
                     /locus_tag="LR726_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL6215189.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76288..77334
                     /gene="holA"
                     /locus_tag="LR726_000072"
     CDS             76288..77334
                     /gene="holA"
                     /locus_tag="LR726_000072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL6215190.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77423..77677)
                     /locus_tag="LR726_000073"
     CDS             complement(77423..77677)
                     /locus_tag="LR726_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL6215191.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78077..78346)
                     /locus_tag="LR726_000074"
     CDS             complement(78077..78346)
                     /locus_tag="LR726_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215192.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78816..79271)
                     /locus_tag="LR726_000075"
     CDS             complement(78816..79271)
                     /locus_tag="LR726_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL6215193.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79409..81337)
                     /locus_tag="LR726_000076"
     CDS             complement(79409..81337)
                     /locus_tag="LR726_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL6215194.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81534..82367)
                     /locus_tag="LR726_000077"
     CDS             complement(81534..82367)
                     /locus_tag="LR726_000077"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL6215195.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82559..83263)
                     /locus_tag="LR726_000078"
     CDS             complement(82559..83263)
                     /locus_tag="LR726_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL6215196.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83286..83912)
                     /locus_tag="LR726_000079"
     CDS             complement(83286..83912)
                     /locus_tag="LR726_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL6215197.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84238..86613
                     /locus_tag="LR726_000080"
     CDS             84238..86613
                     /locus_tag="LR726_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL6215198.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86637..87128)
                     /locus_tag="LR726_000081"
     CDS             complement(86637..87128)
                     /locus_tag="LR726_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL6215199.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87315..88235
                     /locus_tag="LR726_000082"
     CDS             87315..88235
                     /locus_tag="LR726_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL6215200.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT        28609 a        15248 c        18964 g        25470 t
ORIGIN      
        1 catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat ataggaaatg
       61 tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc tgattattta
      121 ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa gggcaatgat
      181 ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac tgtcactttt
      241 ttatccaggt acttggatag ctactttagt agtcttggca ttattactat gtttaattgt
      301 tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta ttttgatgat
      361 aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg cttccggaat
      421 gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg cagttaaaga
      481 gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat taaccgtgct
      541 tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa atccctcgct
      601 ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca cttgcaagtt
      661 tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta catgattgta
      721 cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa cgttcaaaca
      781 aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg ggactcgaac
      841 ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt ccaacacatc
      901 tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag ctcaaaaatg
      961 ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg aatgatctta
     1021 ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct aggaatcatt
     1081 tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga caattaagtt
     1141 ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt ggcaaaattt
     1201 tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa gttctaactt
     1261 tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca aattagaagg
     1321 tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc aagaagaggg
     1381 cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg aagcaccaaa
     1441 cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact cagattccaa
     1501 ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac attttccgaa
     1561 tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca cgatgtgtat
     1621 gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact atccgttgtg
     1681 gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg atgaagaggc
     1741 agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga caaaagctat
     1801 tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat tattagaaga
     1861 aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa tctatgatcg
     1921 cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg atttgttcgt
     1981 tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag taggctggat
     2041 ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga atatgttgtc
     2101 ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg cgctaggcgg
     2161 ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac aaagagaata
     2221 tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa aagctgcttt
     2281 ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg aaaaattcgt
     2341 tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat ttaattggaa
     2401 ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga aacaaacagc
     2461 taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa aaccaaattt
     2521 ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga gttttcttaa
     2581 ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac ttcctaaagt
     2641 aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg aaacgttaat
     2701 cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga cgcaaaaaat
     2761 gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata atgaggaact
     2821 gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga ttgatcatgt
     2881 gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac tgttggatag
     2941 ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt ctgatatttc
     3001 tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg gtgtttgcga
     3061 attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag cattaagcta
     3121 ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga ttagttgtat
     3181 cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt tgagtcgaag
     3241 catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt cgcaaatttt
     3301 ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt tagaacaggt
     3361 gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca atgatttatt
     3421 tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag cattctatga
     3481 aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt agaccattct
     3541 tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag agaatagaat
     3601 gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac atgcaggcta
     3661 tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg agggagaagt
     3721 caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata cacatattca
     3781 cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag tgatgtctga
     3841 aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt caagcaaaga
     3901 acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac caggagctaa
     3961 aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag gggcacaaaa
     4021 cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag aagcttcagg
     4081 tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag agtttgtcaa
     4141 acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta cactagaaga
     4201 agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca atggtatgcg
     4261 tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag aacatgtctt
     4321 ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa tcctaatgga
     4381 aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg gtgggatgcc
     4441 tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa cagcacgtct
     4501 agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta aaaatgttgt
     4561 agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga ttccagcaat
     4621 cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg cagactttat
     4681 cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg aacgtttcca
     4741 cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt tttaaggttt
     4801 tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt aatttcctat
     4861 tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat tagcaaaata
     4921 aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg aacttatttg
     4981 acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact tcaacagttt
     5041 ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa tctgttggaa
     5101 aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt cgagctttca
     5161 tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt tggggattag
     5221 ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat aaaggggata
     5281 agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc ggaacgccta
     5341 tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc aatattggct
     5401 accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag atggaagcta
     5461 aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc ggccttttca
     5521 tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa ggagagataa
     5581 gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag ccgatggatt
     5641 atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca ttaggtgaag
     5701 taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta gtcaaagtgg
     5761 tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg aatattctac
     5821 cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca gctgcaacag
     5881 tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc aatgcatcct
     5941 tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta ttacaaatga
     6001 ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga atcgcatgtt
     6061 acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac cattatgatc
     6121 tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag gaccatgatg
     6181 ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa atgcaggaat
     6241 atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat ctacaagcgg
     6301 cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat gcgatagcag
     6361 agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg acgatccgaa
     6421 catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc agaaattgca
     6481 cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg tttaagggac
     6541 taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa aaagtattga
     6601 cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt catgtacctc
     6661 atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa atgaaaaaag
     6721 gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct attttaagag
     6781 ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat gaaacagagg
     6841 tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc cagcaattag
     6901 tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca gatgaggcat
     6961 tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa actggaaaaa
     7021 cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac atggatattc
     7081 gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta tacttggact
     7141 attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc cgcccattct
     7201 ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt ctttcgttca
     7261 atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt gacagtgagc
     7321 ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata aatgcactta
     7381 ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat ggtatttcgt
     7441 gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct ttttcagtcg
     7501 tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa cgttgcaaag
     7561 tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt atggtttcga
     7621 tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt ctaagccaag
     7681 ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc agatctgcgt
     7741 ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg attacgttca
     7801 tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga tcatcttcga
     7861 ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag atagacgatt
     7921 gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt gacgattgaa
     7981 tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc tagcgtaaat
     8041 attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt cacgatgaat
     8101 acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct ttcatgtttt
     8161 tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa aagtgaatgg
     8221 atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag catttcaaca
     8281 gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc aggtactttg
     8341 atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg ctggataaaa
     8401 tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg aagtttattc
     8461 atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc cccaagctcc
     8521 tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc taaaaaacgg
     8581 tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa atacatggat
     8641 tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc atatagatta
     8701 tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag aaaatgtttt
     8761 ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag attccttcat
     8821 ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct tgtggttcaa
     8881 gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg atgcctacgc
     8941 taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact tgattgtcaa
     9001 tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca taataaatag
     9061 ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa aattcttgtc
     9121 ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg taacttgggt
     9181 ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct aaattcgatg
     9241 caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca gtctgcagct
     9301 ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt ttatctgaca
     9361 cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa acaatcaaag
     9421 aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt aatggttttt
     9481 tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat tttaacatga
     9541 aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa aacaccactg
     9601 agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg tagaaatgga
     9661 aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt atacattgct
     9721 gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg attttgatgg
     9781 cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat ttggttttct
     9841 aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg gaggcatatc
     9901 gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac atgccgaatc
     9961 aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga tcacaccgat
    10021 ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca ttagtcgtcc
    10081 agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag tattgatcat
    10141 tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa aagcttaaag
    10201 gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg cagctttgat
    10261 tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac cggatgaagc
    10321 gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg aaagcaataa
    10381 caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc aacgttcgaa
    10441 tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt atacggaaca
    10501 aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat ctgtagaaga
    10561 gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg aaaatgaagc
    10621 aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg tagaagaaat
    10681 ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg tagaccttgc
    10741 aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca aaaacggtta
    10801 tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag atatagcaga
    10861 agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag aatcacaagc
    10921 agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag aactgaaatt
    10981 agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag cctacaattt
    11041 agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta aaatcgtaga
    11101 acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa agcaatatga
    11161 ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag aagcacaagc
    11221 tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg aagcactggc
    11281 agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag cagcactagc
    11341 aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca aggaatatgg
    11401 cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc gcgaagcagc
    11461 acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca gttcaggaga
    11521 aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt ctactcaaga
    11581 aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt tgtcgacacg
    11641 tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac aaaactcgat
    11701 gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa gactttttaa
    11761 agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg ctatttttct
    11821 tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat cgttctttct
    11881 tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca ggaggacgaa
    11941 gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt acacgttgga
    12001 tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac aacgtagcta
    12061 ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc acaataataa
    12121 ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag cttttgacaa
    12181 aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta gactttccaa
    12241 aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat gcagcaatca
    12301 tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca gctaaggcac
    12361 ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt ctcctttttg
    12421 gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt attcaagtat
    12481 aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc aaaacagtga
    12541 tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat tggccagctg
    12601 cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg cgtacgggca
    12661 tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt ttttttgctg
    12721 ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg cttgcctctc
    12781 gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat tatttagtgg
    12841 aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg attggattag
    12901 tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg aaggaaaacc
    12961 aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa tttcttttat
    13021 cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc attcgcgaaa
    13081 gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg ctatactacg
    13141 aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg ctggaaaatc
    13201 tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta ttgatttata
    13261 ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt attgaagaag
    13321 tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc gaggatcgag
    13381 cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag gcagaattct
    13441 cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg tcaaaaagct
    13501 gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc acaagtcaca
    13561 atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc ggagctgaac
    13621 ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc ggtaataagt
    13681 ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca
    13741 gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt gcatttgatc
    13801 caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt caccttcaat
    13861 catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc cgctttcgat
    13921 gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg ggtaatcagc
    13981 aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt ttgcctgaaa
    14041 agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga cggattgttc
    14101 gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt gagagctcaa
    14161 aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc ctccagctaa
    14221 ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga aaggtgcttg
    14281 gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact gctgataaag
    14341 tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt ctgctaagcg
    14401 catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata gaacgtgttt
    14461 gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta cagcaatgta
    14521 cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag cttttggaat
    14581 attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa ctgctgttag
    14641 ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac caagaccaac
    14701 gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat tgagtttctt
    14761 gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact tgaaacatta
    14821 aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag tagaatagtt
    14881 aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt gtattattag
    14941 aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt ccagaacaat
    15001 ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag aaagtaaaaa
    15061 atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa acgattattt
    15121 ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca cgcatcgcgc
    15181 aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa aataatcagc
    15241 aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg ggctatgatg
    15301 atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga gaaaaaatcg
    15361 aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag gcgaatgggg
    15421 aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt tggagcagtt
    15481 gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc gcatgttcaa
    15541 gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa agaaaagaac
    15601 agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac gtccaaacaa
    15661 ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga tcacaaatta
    15721 gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata cttgatcgac
    15781 aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc tggatttcca
    15841 ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt cgaacaaaat
    15901 gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa gattactaaa
    15961 gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt cgatgtcgtc
    16021 gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta cggagatcag
    16081 cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt tcgttatcca
    16141 aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg ttatgtcggt
    16201 aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata tctgaatgaa
    16261 ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca aaagcgcaag
    16321 aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc ctttgttttt
    16381 tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga agcgcaatcc
    16441 catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga tggttatgga
    16501 gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg aaaaagtacc
    16561 ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca gaagaaagta
    16621 agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg agacttcaaa
    16681 gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt ccaaggagta
    16741 gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca ggaagccgca
    16801 caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa aatcatccag
    16861 gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag aagtggctga
    16921 tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc gtaagagtac
    16981 cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc cgttcgatca
    17041 tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta attgctgtgt
    17101 atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct gacattccag
    17161 ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac atgagcatgt
    17221 tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct ttagagacag
    17281 taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg tcgaaatata
    17341 cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt gcaggtaaag
    17401 aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct atcattgcga
    17461 caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca aatgccatca
    17521 catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa tatcgggaaa
    17581 ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga gcagatagtg
    17641 gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa aaagagaaat
    17701 ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg ttattattag
    17761 cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg aaaaacgaat
    17821 aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg cttgaaaagt
    17881 cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac gtgtatattg
    17941 tacctaattt catacagaat tactagttat ttttctgttt tttagggaag agcgcacacg
    18001 aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat atcctttttt
    18061 ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat gaaagaagaa
    18121 tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca gaacgataag
    18181 gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt tctgtataaa
    18241 aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa gaaaagaatg
    18301 agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg gcaagaagat
    18361 gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca cgatgcaaca
    18421 gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga ttatgaattt
    18481 tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga tgatatctgg
    18541 gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga accattagat
    18601 gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta tttagcaacg
    18661 acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt tgcgattgct
    18721 tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa ttggacatgg
    18781 gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta tgctatttca
    18841 agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga gaaaggattg
    18901 aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc agactatcta
    18961 acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc agaaccaggt
    19021 ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag agaagcggca
    19081 atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc tgaagatatg
    19141 gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg tatcccttta
    19201 cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga agctgctaag
    19261 tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg agaacgtggt
    19321 gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc aactttgcaa
    19381 gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt gaatgtcttt
    19441 gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga aaaagtcatt
    19501 tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc gtcaaacatt
    19561 ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt cttggatcaa
    19621 gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct attgataaca
    19681 gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt gggaaatcca
    19741 gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg ttttttgaac
    19801 tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact gatctttggt
    19861 ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt tcgttctttg
    19921 tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg gcgggagttg
    19981 ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc tgcgctttcc
    20041 tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc ttggcaattc
    20101 ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc gtattatgaa
    20161 gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac gttacctctt
    20221 ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt tatgactttt
    20281 acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt ttatgctctt
    20341 tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc tatttcgtgg
    20401 atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc aaaaaaatgg
    20461 gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat ggtttcaata
    20521 cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga tgatcgcagg
    20581 ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc cagagacttt
    20641 tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat tttttacttt
    20701 ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc tgagctcgac
    20761 ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct ggttcgtcgt
    20821 catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt ttattattta
    20881 caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt tttttggaca
    20941 agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag agttagatga
    21001 atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta tttttccttt
    21061 gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat gggacgactt
    21121 catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga ttgccatcaa
    21181 attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga tgtctacgtt
    21241 gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg tccaggggat
    21301 cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat gtttctttac
    21361 aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa tgctcttttc
    21421 gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg tgttgtctat
    21481 ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta cttggcagaa
    21541 aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat tacgttgttt
    21601 cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa gattggagct
    21661 tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag tttttttagt
    21721 acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt gatcaaaagg
    21781 aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg ccttttgtat
    21841 ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta tccagggctg
    21901 ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg aagaaaaaat
    21961 atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt gcctctacgt
    22021 atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg atggattatg
    22081 caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt atgccatcac
    22141 aatttgagca actactggat gacgtacggc cggctgaagt cattattacg tcagtagatc
    22201 gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt atttgcgaaa
    22261 aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa aaacagacgg
    22321 gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg aagataaagg
    22381 aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa tggctgttaa
    22441 atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac aatagcggcg
    22501 ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg ttgaacagcc
    22561 gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat aatgcggaga
    22621 aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct aaagctgatc
    22681 catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta gaagcagaaa
    22741 aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct attgaagata
    22801 cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg ctaaacgcgt
    22861 atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg atcgaaatga
    22921 aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa ggagcagccg
    22981 ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta atcgtcgaac
    23041 aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta tttggtgaag
    23101 aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt ttgtcgattt
    23161 tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg attgacgagc
    23221 ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag tttataaaat
    23281 cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa aggggctatg
    23341 acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag gagaaaataa
    23401 aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt agattttaca
    23461 aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt tttcaatatt
    23521 tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa cagttgcaag
    23581 aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc ataatatgga
    23641 tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg gctatttcac
    23701 ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat agtaggtttt
    23761 tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa agtgaaagaa
    23821 ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt aaaagtcgac
    23881 agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa acggtttgca
    23941 gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc tgggatcgca
    24001 ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag aaaagcaaaa
    24061 agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac gaaacaagtg
    24121 acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt attgattatc
    24181 gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg ccagcaagct
    24241 ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga tggacgtcag
    24301 ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc cttgacgaat
    24361 ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca caaaatggaa
    24421 aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca gtagcggtgc
    24481 ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat ttgatttcaa
    24541 ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac cgatttttcg
    24601 gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca ctgatcttga
    24661 tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct gggatctttg
    24721 ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct atagtaacag
    24781 gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag aaaatgggtg
    24841 gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt gcatttgtga
    24901 cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt tcaatcgcgg
    24961 tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg attgatctga
    25021 gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt ggtaaaccaa
    25081 ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc atggtagaat
    25141 caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga gaagaagaat
    25201 tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt ttcaatacat
    25261 tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga atcaaaacaa
    25321 gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc cctaaaatcg
    25381 gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta gtcatgttcg
    25441 ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc aatgataaaa
    25501 atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt acgccgacat
    25561 tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc gtaatgagta
    25621 gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat gaagaaatat
    25681 tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca gcgttttgac
    25741 ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt tgtttgagct
    25801 aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc ctgccgttca
    25861 tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg aatattgttc
    25921 cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa tgtgacctaa
    25981 cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat gtttttcttt
    26041 gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat gaatgtagaa
    26101 aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag tacttcagat
    26161 tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc ttatgaaaaa
    26221 aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca aaacgctcga
    26281 aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct gccgggtatg
    26341 gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg aacattaaat
    26401 ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt agctactaca
    26461 gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat gctttccatg
    26521 tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc taaaactgtt
    26581 atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga tcgggatcgt
    26641 cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg gctttaaagt
    26701 aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt ggcatattga
    26761 aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag atgtcattac
    26821 ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg attttaccca tgtcatttat
    26881 tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa aatctttttt
    26941 ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga cggatatcca
    27001 agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag gcggctatga
    27061 tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca tggacctttt
    27121 aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga tttcgatcat
    27181 ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa acatccacca
    27241 caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg tgatcgagga
    27301 ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg ttttaggggt
    27361 agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc ctcgtcctca
    27421 caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg cccatattcg
    27481 aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg cagtaatggt
    27541 caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata agccagactg
    27601 gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg gacatatcac
    27661 tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga atatttggga
    27721 ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga atatctggac
    27781 ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg aagcttgggc
    27841 agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg catcatttga
    27901 tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg cgtttacacg
    27961 agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc taacaagtac
    28021 ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata ttttacggga
    28081 agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca aatatacagt
    28141 gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat tgaaacttgc
    28201 cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg caaaaggagt
    28261 agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc catttattga
    28321 agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc aagttctccc
    28381 tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa gtattgaacg
    28441 ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag aagaattttt
    28501 tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga tcggttcaga
    28561 aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct atgaaaatgt
    28621 cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca tcttgcctga
    28681 taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta aaaatctaac
    28741 ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga tttatagcca
    28801 gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt atgatcttgt
    28861 tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc tgttagaaga
    28921 agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct ttgattatca
    28981 tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat aaagaagagt
    29041 gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa gcagatccgt
    29101 tagcggttct gcttctttta gtttattata ttttcttatc aataatagga acctgatttt
    29161 aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga attaagcgta
    29221 aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca agttttcgag
    29281 aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat gatttttgaa
    29341 acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg tctgataaag
    29401 aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt ttcggaatgt
    29461 ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca aatcattata
    29521 tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt gttttattgc
    29581 atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt aattctaata
    29641 taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc gtttctgata
    29701 aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt gatttataaa
    29761 aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa
    29821 tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga aggaaaagcc
    29881 aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt agatcaagca
    29941 actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt gaataatcaa
    30001 atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca ttttattgaa
    30061 caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct ggaagtggtt
    30121 atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga aggtaaagaa
    30181 ttcatcaaac caatcattga attctactat aaggatgacc agttggatga tccatttatc
    30241 aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag tgagctgaaa
    30301 gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt cggtttggtt
    30361 ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct tgcggatgaa
    30421 gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat ggacaaagac
    30481 gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata taaccgtcta
    30541 tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta tatgtaacct
    30601 ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg
    30661 gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa cttgctgatg
    30721 cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa
    30781 atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat gaaatttgcg
    30841 gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt tcgcgagatc
    30901 atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg atttgatggg
    30961 gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc gattgctcgg
    31021 ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc agtattcggt
    31081 acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt actgagacga
    31141 aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc caagacggcc
    31201 tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca tggggaagga
    31261 aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca gattgccttt
    31321 acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac gaacaaagca
    31381 gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt gctaggatca
    31441 gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag
    31501 atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa
    31561 tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg aagaatgcct
    31621 aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc ctataaaaat
    31681 tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca aggccctgga
    31741 gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa agcagaaagt
    31801 cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt tggcggaatc
    31861 atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc attgaggttc
    31921 ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc aggtatcggc
    31981 ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt tgatccttgt
    32041 tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca caaacatatc
    32101 cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt
    32161 cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga agaagaacag
    32221 caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct tgaagcgtgt
    32281 ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat gggagcagca
    32341 ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact tatattgaca
    32401 ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat gttatcggaa
    32461 tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta
    32521 tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga cgggcaatat
    32581 cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt agctgaagat
    32641 gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta
    32701 gctccttatc tgccagttat cgaagaacct ggacaagtat tgcttgatct gctgcagcaa
    32761 ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt acaaaccaat
    32821 acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac gaataaagcg
    32881 ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa aatcggagga
    32941 caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa gcccttggcg
    33001 atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg ggaattatcg
    33061 acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt tatttcagga
    33121 aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc aatgattggt
    33181 atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa aaaatcaggg
    33241 gatctaatct atatattagg taagacgttt gcagattttg atggcagtga attacaaaag
    33301 atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat tgaaaagaga
    33361 aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc tcatgactgt
    33421 tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca gctagggata
    33481 tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc aagatttgtg
    33541 ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga tgctgctgtt
    33601 ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga cggtcagatc
    33661 aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg tcttatgaag
    33721 tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca gacgccgcta
    33781 gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt gcaggtatcg
    33841 tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt tctgaggtat
    33901 tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga catgtacgtt
    33961 atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc aaatttcatg
    34021 atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc cttagaaaag
    34081 agttagagga aaatggggcg atcttccatt ctaactccga tacggagata ttgatgcatt
    34141 tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct ttgaatgaag
    34201 tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc gctttggacc
    34261 caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac gtgattgcct
    34321 cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc cagcctggtg
    34381 agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag gacacgcagc
    34441 tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac atcgcaggga
    34501 tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa
    34561 aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc ggttatgcgg
    34621 aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat
    34681 ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta tctgctgtac
    34741 gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt cggggtacga
    34801 caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc catgtgagga
    34861 ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa acgcgtaaag
    34921 aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt gcagattctt
    34981 taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt
    35041 attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta tatgactatg
    35101 aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga
    35161 agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct
    35221 ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg tttaggtatg
    35281 atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca attaaaagag
    35341 ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc aatcgaagca
    35401 gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga tatcttagca
    35461 caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa tgatccgtta
    35521 cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg
    35581 attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta tgatctagcc
    35641 ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac gatcaaccca
    35701 ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta ctctcttgtt
    35761 cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc agaattgaaa
    35821 aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc cttgcttccg
    35881 ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg gtttgtagaa
    35941 aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg ctcatggccg
    36001 attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat
    36061 gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa agtaaaagaa
    36121 atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc
    36181 aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct gtttttgctt
    36241 ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa ggattggaat
    36301 cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg
    36361 cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg
    36421 aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta ttagctggat
    36481 atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc atcaatatcc
    36541 atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc tttgaagctg
    36601 gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat actggaccaa
    36661 tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct ttagaagaaa
    36721 aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt attgaaaatg
    36781 gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg ggattgtagc
    36841 attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag gaacaaagca
    36901 tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga
    36961 aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc ttttaggaag
    37021 acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac caatcgactt
    37081 tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg tcgaaatagc
    37141 agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa
    37201 ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac tttcagaatt
    37261 acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa aagtctttcg
    37321 tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg tggaagaaaa
    37381 agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa
    37441 tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc
    37501 agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg cagatgctgc
    37561 gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac acatgaatcc
    37621 ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga
    37681 tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc tgccaacagc
    37741 agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata cacaagaagc
    37801 tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact ttgcgcagga
    37861 agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg tacaagatca
    37921 agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta agccgactcc
    37981 agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga aatctaatgc
    38041 gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga tgaatcgagt
    38101 cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg cagttttagc
    38161 tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac atggtataaa
    38221 agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata tggcgaataa
    38281 atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga gaggagtcgg
    38341 ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact
    38401 tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa
    38461 agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa tccattttgt
    38521 taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt taaatgggat
    38581 cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag cagcagctct
    38641 gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa tcccgacagc
    38701 taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc
    38761 gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg tggctaccac
    38821 agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat ttggggatag
    38881 tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc ttttggcgtt
    38941 tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc gggcatttga
    39001 taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac cgcaaatttc
    39061 aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa
    39121 agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg
    39181 accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc
    39241 tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc gtacagtaga
    39301 gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg ccggttaccc
    39361 cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc tttctgttta
    39421 ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac gtctttattt
    39481 agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat
    39541 ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag ctgccaatag
    39601 cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac aaacataatc
    39661 ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt tgttgtgtat
    39721 ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat cagaaagcag
    39781 ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta aaagtgattg
    39841 agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat ggaaacggtg
    39901 aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga gaagatcttt
    39961 ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct aaaaaacata
    40021 tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg gatgctttac
    40081 ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg tttgatgaat
    40141 tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg
    40201 aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa tttggcttga
    40261 aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa gcaagtatcg
    40321 atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc caagcatggt
    40381 caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat
    40441 taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact ggcttggctt
    40501 ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg atgaacgtag
    40561 ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa gattggtacg
    40621 aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca ggtcaaaagg
    40681 gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag atattctgtc
    40741 tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta gttatttttg
    40801 tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt tttcttaaat
    40861 aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc tgaaatacga
    40921 tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt cagataatcg
    40981 atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg acttaatgca
    41041 acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc tttgataaaa
    41101 tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga gggcagagca
    41161 gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt
    41221 gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt cttttctttc
    41281 aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg tgttcgagtt
    41341 ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt tttcgataat
    41401 gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag tttcgcttct
    41461 tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt ttcaagtatc
    41521 atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac aggaccaatg
    41581 gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt ttgggctacc
    41641 tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa tttgatgaat
    41701 cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa cttcaaataa
    41761 tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc taataatacg
    41821 gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa tgtttcactg
    41881 atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca
    41941 agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac tgctttttct
    42001 attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc tgcctgtacc
    42061 tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat gagacgagtc
    42121 caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg
    42181 atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc catctcacca
    42241 tctccagctt cgccgacata agtgattgga taataagaac caaggacagt tatttgttcc
    42301 cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata catgtttgtt
    42361 tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc cttctcggta
    42421 tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga gacgcttgtt
    42481 tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg ctaactcttt
    42541 acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa ctttgaattg
    42601 gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac ggacgtactt
    42661 cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta aaatagaaac
    42721 aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt atcttatttt
    42781 taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa tccagtcttg
    42841 tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc
    42901 ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat aaataaaatg
    42961 gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt attgtttcaa
    43021 agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc tagcggtaag
    43081 tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag
    43141 ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct ctaaaaagcg
    43201 acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca ctattgttaa
    43261 tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga tctccacacg
    43321 aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct gattgatgat
    43381 ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct cgttttgata
    43441 atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt ctttaagtgt
    43501 atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga tattttcctc
    43561 attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt ccaaaacttt
    43621 ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg tctgttgata
    43681 aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa cagcaaatag
    43741 aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat
    43801 acttctccag tcaataattt ctggttggga tgttagctat attctgctgg gaaaagacag
    43861 tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt ccaaaataac
    43921 gagtaacggt attggctaca taccagccga catcaggttt acactcgact acacaattga
    43981 cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt acaatgatca
    44041 aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg tgagtcattt
    44101 catcactatg gctgtataat acatcgattt cggataaatc atttgtttct aaatatcgtt
    44161 tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga acaatagtca
    44221 tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga ttgattgatt
    44281 ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca gcaagttcta
    44341 ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat aaacccgccc
    44401 gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg
    44461 tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct tcttttaata
    44521 cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact ttataggcaa
    44581 tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt cggtagagaa
    44641 cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt ttccgccagc
    44701 tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat
    44761 taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa aataaaacac
    44821 gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga taaaagggaa
    44881 aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca
    44941 aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt
    45001 ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga aaaacaaatt
    45061 tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg
    45121 caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc cgaccaaatc
    45181 agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa aactgggtta
    45241 taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac aaatagaaaa
    45301 tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt cggctttaac
    45361 tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg atcgtttact
    45421 gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag taggtgtatc
    45481 gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac cattcacgat
    45541 tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca acggagtgat
    45601 gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg gacagacgag
    45661 tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa ggatggataa
    45721 tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc catatgatcc
    45781 aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc
    45841 tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga tcattgccgg
    45901 ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata cgattgaaat
    45961 gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa cttatgataa
    46021 tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata aagataatta
    46081 tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa attaagagga
    46141 accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat ggcggattgt
    46201 attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg
    46261 aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa
    46321 tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga cggggaaatc
    46381 ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc
    46441 gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga gaatattttt
    46501 ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga aaagaaaaca
    46561 tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt atcccagatt
    46621 ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc cgtccgtctg
    46681 ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga
    46741 ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca taagctgaat
    46801 gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac gattgagaca
    46861 ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg cagagattta
    46921 gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa agtagaagac
    46981 tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat caacttgcat
    47041 ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag aactgaattc
    47101 gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat attaaaggaa
    47161 ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact cgcttatgtc
    47221 tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga aaacgtgacg
    47281 ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa agaagtaact
    47341 gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca tcaaattgtc
    47401 agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa
    47461 cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc aaaatatgag
    47521 atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct tatctcttct
    47581 gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga aggacgtatt
    47641 acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt aatgacgaaa
    47701 gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag agaaaaaact
    47761 aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga tcattttagc
    47821 gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc cattgaatat
    47881 aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga tgttattagt
    47941 tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg ttggtgcaat
    48001 tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta tcgcatgtct
    48061 tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg cagggatacc
    48121 ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac aagttgttct
    48181 aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta cgggttattt
    48241 accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag gaatgatcat
    48301 cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt
    48361 tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag tcgttgtact
    48421 aggaataact tatatctttg cctcttatca agggtatccg attattttga tcattcttgg
    48481 tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc gacagattta
    48541 tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa agcgaatcac
    48601 gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag ttttagctgc
    48661 tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg caatggcggc
    48721 agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg gtgcgattgt
    48781 cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg tcggggtaga
    48841 ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag acttgtataa
    48901 taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac tgtgacaaga
    48961 catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt tgtatagact
    49021 aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct tgattttttt
    49081 gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat taataatgtt
    49141 cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt gattacgaag
    49201 atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt gatacaacgg
    49261 tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta
    49321 ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc atgcatcggt
    49381 ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct
    49441 cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg gcagaagaat
    49501 cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac tcagttattg
    49561 atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg
    49621 gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca aaagtcggga
    49681 ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact ggaggctggc
    49741 aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg
    49801 ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca atggtcatga
    49861 ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa
    49921 tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg
    49981 ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca ctagtggaaa
    50041 tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg gaagccattc
    50101 gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa
    50161 taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca
    50221 aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag atacaagctt
    50281 ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag tcccttatgt
    50341 tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag aaaaaataga
    50401 caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg
    50461 tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc ctggaagtca
    50521 acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca ccgttgtcca
    50581 tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca gcggatggtt
    50641 tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc ggcaaatgat
    50701 ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag caaagcagat
    50761 ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc ttttgccgaa
    50821 ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag aaatggatct
    50881 tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta aatttgaatt
    50941 ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa tctttttgat
    51001 tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt ctttttttta
    51061 ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga tggatgaaga
    51121 agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga aagcagtgct
    51181 tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac gacgagaaaa
    51241 gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg tatttacgga
    51301 tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc cggataatgt
    51361 gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat
    51421 aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat
    51481 gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg atttgaaaat
    51541 gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt ggggagattc
    51601 acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga ttacttggga
    51661 tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc ttggaattgc
    51721 accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca gtaggctgga
    51781 tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga
    51841 ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa tttcaagctg
    51901 tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac cattatttgt
    51961 acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta taacaccaac
    52021 gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa atgcagatga
    52081 aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata tgcggattgg
    52141 cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg
    52201 ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca
    52261 gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag
    52321 caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag
    52381 cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg
    52441 aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag
    52501 cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc
    52561 agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg
    52621 tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac
    52681 atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg
    52741 gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa
    52801 atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa
    52861 tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg
    52921 cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg
    52981 cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta tatactgtat
    53041 ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga
    53101 cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag
    53161 acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg
    53221 aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa
    53281 tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga
    53341 aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga
    53401 aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc
    53461 acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg
    53521 aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga
    53581 tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt
    53641 tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt
    53701 agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac ccatcacaat
    53761 tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg
    53821 tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa
    53881 aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa
    53941 actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa
    54001 agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg
    54061 tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga
    54121 tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg
    54181 tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc
    54241 tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc
    54301 tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat
    54361 cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat
    54421 ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa
    54481 atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa
    54541 tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa atgtgattgt
    54601 tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg caccaggaca
    54661 cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag ctaaaccggc
    54721 tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag tcttccaatt
    54781 taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa
    54841 accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg acaaatcagt
    54901 agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac
    54961 tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc
    55021 gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc
    55081 aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca
    55141 atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag
    55201 agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa
    55261 atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc
    55321 agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat
    55381 gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca
    55441 cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa
    55501 agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt
    55561 aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga
    55621 aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc acggactata
    55681 tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa
    55741 tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac
    55801 aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta
    55861 tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga
    55921 aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt
    55981 agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga
    56041 gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag
    56101 atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa
    56161 tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg
    56221 gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt
    56281 ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat
    56341 ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac
    56401 ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca
    56461 ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc
    56521 gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat
    56581 tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg
    56641 aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac
    56701 aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa
    56761 aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg
    56821 tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg
    56881 tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag
    56941 taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct
    57001 atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa
    57061 aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg
    57121 cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag
    57181 atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat
    57241 cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta
    57301 ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa
    57361 tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg
    57421 gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat atttgatttg
    57481 aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa
    57541 taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg
    57601 ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact
    57661 tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga
    57721 acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa
    57781 aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc
    57841 cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag
    57901 aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa
    57961 gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga
    58021 acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat
    58081 tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat
    58141 ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa
    58201 ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc
    58261 atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa
    58321 aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg
    58381 aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg
    58441 gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac
    58501 attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa
    58561 gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa
    58621 tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa
    58681 ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt
    58741 tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag
    58801 tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt
    58861 atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat
    58921 tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac
    58981 gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca
    59041 cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact
    59101 tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg
    59161 cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata
    59221 tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg
    59281 tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc
    59341 ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc
    59401 aagaaccaac catggcacct atttttgata caattattga aaaaatccct gctcctgtcg
    59461 ataacagcga cgaaccatta caattccaag tttcattact tgactacaac gattatgtag
    59521 gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat
    59581 taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg
    59641 gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta
    59701 tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta
    59761 tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg
    59821 ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac
    59881 ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt
    59941 ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg
    60001 aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac
    60061 cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct
    60121 tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt
    60181 tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga
    60241 cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc
    60301 aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt
    60361 attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat
    60421 atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga
    60481 aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa
    60541 aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg
    60601 aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa
    60661 aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata
    60721 aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa
    60781 tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt
    60841 ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt
    60901 cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa
    60961 acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat
    61021 tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca
    61081 gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat
    61141 gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc
    61201 gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt
    61261 actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg
    61321 gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt
    61381 ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat
    61441 tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa
    61501 aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt
    61561 aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca
    61621 atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa
    61681 aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga
    61741 aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg
    61801 tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct
    61861 agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat
    61921 cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag
    61981 agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta
    62041 tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg
    62101 caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca
    62161 gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa
    62221 agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac
    62281 ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat
    62341 atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag
    62401 agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca
    62461 gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga
    62521 tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga
    62581 aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca
    62641 aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa
    62701 cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc
    62761 tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg
    62821 taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca
    62881 gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa
    62941 gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct
    63001 gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga
    63061 gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc
    63121 aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct
    63181 tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt
    63241 atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt
    63301 gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa
    63361 accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt
    63421 tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc
    63481 aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa
    63541 gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta
    63601 tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt
    63661 tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag
    63721 gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa
    63781 agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt
    63841 tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg
    63901 gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag
    63961 cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt
    64021 tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa
    64081 aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat
    64141 ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt
    64201 taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca
    64261 tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt
    64321 tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct
    64381 tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga atattccgta
    64441 caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg
    64501 tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat
    64561 ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc
    64621 atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca
    64681 aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct
    64741 tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca
    64801 gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag
    64861 ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt
    64921 gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga
    64981 aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta
    65041 gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct
    65101 gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa
    65161 ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca
    65221 gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat
    65281 ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt
    65341 caatgccgaa tcactacaga agacccgttg aataactttt taccagacac agggaaaatc
    65401 gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg
    65461 ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct
    65521 acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga
    65581 gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga
    65641 aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga
    65701 gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt
    65761 atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca
    65821 agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc
    65881 gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat
    65941 caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact
    66001 ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg
    66061 gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg
    66121 cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat
    66181 cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt
    66241 cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg
    66301 gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt
    66361 cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt
    66421 gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt
    66481 ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc
    66541 agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac
    66601 gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat
    66661 gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac
    66721 cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct
    66781 gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag
    66841 gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc
    66901 aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg
    66961 gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca
    67021 ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc
    67081 ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt
    67141 ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa
    67201 attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt
    67261 ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca
    67321 tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg
    67381 cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc
    67441 ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg
    67501 gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct
    67561 ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg
    67621 atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc
    67681 gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg
    67741 actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga
    67801 ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg
    67861 aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg
    67921 agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg
    67981 attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg
    68041 aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg
    68101 ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc
    68161 aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag
    68221 aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata
    68281 acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt
    68341 atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc
    68401 cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa
    68461 aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa
    68521 cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg
    68581 aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga
    68641 cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag
    68701 aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt
    68761 ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca
    68821 ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg
    68881 atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct
    68941 gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa
    69001 gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa
    69061 aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga
    69121 aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca
    69181 cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa
    69241 atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt
    69301 aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt
    69361 atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt
    69421 ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga
    69481 agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat
    69541 cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga
    69601 aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag
    69661 cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt
    69721 tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag
    69781 gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag
    69841 ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat
    69901 tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc
    69961 atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa
    70021 ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac
    70081 ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac
    70141 aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag
    70201 ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc
    70261 aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc
    70321 aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac
    70381 ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc
    70441 aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca
    70501 tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga
    70561 gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg
    70621 agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc
    70681 aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg
    70741 agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc
    70801 aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca
    70861 ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc
    70921 tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta
    70981 cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt
    71041 ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc
    71101 aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac
    71161 ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa
    71221 catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa
    71281 gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa
    71341 tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt
    71401 taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat
    71461 cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt
    71521 tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg
    71581 caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa
    71641 aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt
    71701 cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg
    71761 ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc
    71821 ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt
    71881 tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa
    71941 aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt
    72001 ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat
    72061 agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat
    72121 agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga
    72181 aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc
    72241 atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat
    72301 caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga
    72361 tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc
    72421 tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga
    72481 acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc
    72541 caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc
    72601 agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga
    72661 agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt
    72721 tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga
    72781 tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag
    72841 gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc
    72901 aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag
    72961 ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc
    73021 tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa
    73081 tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat
    73141 cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga
    73201 atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat
    73261 acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga
    73321 cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa
    73381 agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata
    73441 aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa
    73501 gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc
    73561 tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat
    73621 cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg
    73681 ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca
    73741 gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg
    73801 tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga
    73861 ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca
    73921 agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt
    73981 ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga
    74041 ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt
    74101 ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca
    74161 taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat
    74221 ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg
    74281 aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag
    74341 cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg
    74401 agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca
    74461 ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa
    74521 atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg
    74581 aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag
    74641 gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat
    74701 cgggtctaca catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat
    74761 ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg
    74821 caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca
    74881 aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt
    74941 tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt
    75001 ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc
    75061 tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag
    75121 gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt
    75181 tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg
    75241 gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac
    75301 gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt
    75361 tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga
    75421 ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca
    75481 gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt
    75541 tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata
    75601 tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg
    75661 acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa
    75721 tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc
    75781 aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg
    75841 atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc
    75901 tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg
    75961 aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc
    76021 acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga
    76081 ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac
    76141 tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct
    76201 ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag taattctcat
    76261 ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta
    76321 caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt
    76381 ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac
    76441 ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca
    76501 gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta
    76561 acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg
    76621 aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa
    76681 cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg
    76741 aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg
    76801 gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg
    76861 agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa
    76921 gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt
    76981 ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa
    77041 gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa
    77101 tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca
    77161 tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag
    77221 ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa
    77281 ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa
    77341 agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa
    77401 aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc
    77461 ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag
    77521 cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt
    77581 tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg
    77641 tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa
    77701 gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct
    77761 ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca
    77821 gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag
    77881 cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg
    77941 gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac
    78001 gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc
    78061 ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt
    78121 ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca
    78181 gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca
    78241 ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt
    78301 cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt
    78361 cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg
    78421 atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc
    78481 caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt
    78541 acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat
    78601 aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag
    78661 ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact
    78721 ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg
    78781 gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt
    78841 aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag
    78901 acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc
    78961 aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag
    79021 ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata
    79081 gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc
    79141 caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct
    79201 atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc
    79261 caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa
    79321 agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa
    79381 taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata
    79441 gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac
    79501 gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta
    79561 gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa
    79621 taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt
    79681 tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt
    79741 gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac
    79801 caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt
    79861 ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg
    79921 tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa
    79981 ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct
    80041 gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg
    80101 caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt
    80161 gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt
    80221 ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg
    80281 tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa
    80341 agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg
    80401 cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg
    80461 cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag
    80521 aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc caacctagga
    80581 aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat
    80641 tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta
    80701 attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg
    80761 gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca
    80821 cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat
    80881 ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa
    80941 catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat
    81001 tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc
    81061 ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac
    81121 caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt
    81181 tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg
    81241 gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc
    81301 cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa
    81361 acaaggatag cataataaaa atgtaattga taataataac agctaaatga gaacgataat
    81421 ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa
    81481 agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt
    81541 gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg
    81601 gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca
    81661 aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct
    81721 tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag
    81781 aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc
    81841 gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga
    81901 agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg
    81961 cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag
    82021 aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt
    82081 atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat
    82141 ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc
    82201 cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg
    82261 aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct
    82321 tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt
    82381 cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt
    82441 aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca
    82501 aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt
    82561 atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc
    82621 cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc
    82681 cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat
    82741 gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa
    82801 atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta
    82861 agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat
    82921 atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca tatccacctt
    82981 caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc
    83041 ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag
    83101 aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt
    83161 gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca
    83221 tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag
    83281 tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg
    83341 ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta
    83401 tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac
    83461 agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc
    83521 cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat
    83581 tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa
    83641 agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt
    83701 tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc
    83761 aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct
    83821 tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca
    83881 atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt
    83941 ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc
    84001 tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa
    84061 agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa
    84121 attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag
    84181 ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg
    84241 atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt
    84301 atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag
    84361 ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa
    84421 ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt
    84481 atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa
    84541 caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat
    84601 gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg
    84661 agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa
    84721 gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg
    84781 atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct
    84841 gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga
    84901 aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag
    84961 atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca
    85021 ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc
    85081 tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat
    85141 cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc
    85201 atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt
    85261 atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta
    85321 agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat
    85381 aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa
    85441 cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta
    85501 ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat
    85561 caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa
    85621 gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt
    85681 gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc
    85741 gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt
    85801 gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc
    85861 tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct
    85921 ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa
    85981 agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc
    86041 gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc
    86101 agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa
    86161 cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc
    86221 gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt
    86281 atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat
    86341 tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa
    86401 gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg
    86461 gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca
    86521 gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca
    86581 aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa
    86641 cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca
    86701 gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct
    86761 tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg
    86821 attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg
    86881 gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct
    86941 ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat
    87001 ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc
    87061 tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct
    87121 tttgccatag gaaccctcct gatatacata taaatattta ccatacatct atcttatcat
    87181 aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt
    87241 ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc
    87301 ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta
    87361 ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg
    87421 tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt
    87481 ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat
    87541 tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg
    87601 tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg
    87661 ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta
    87721 caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg
    87781 gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact
    87841 gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca
    87901 tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa
    87961 cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct
    88021 attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc
    88081 ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt
    88141 cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat
    88201 tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa
    88261 tttaatctat tacatctaat aagaacattt t
//
LOCUS       DAFGFZ010000002        61186 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000002 DAFGFZ010000000
VERSION     DAFGFZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.002"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(179..1372)
                     /locus_tag="LR726_000083"
     CDS             complement(179..1372)
                     /locus_tag="LR726_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6215201.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(1456..1656)
                     /locus_tag="LR726_000084"
     CDS             complement(1456..1656)
                     /locus_tag="LR726_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBL6215202.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(2080..2313)
                     /locus_tag="LR726_000085"
     CDS             complement(2080..2313)
                     /locus_tag="LR726_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215203.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(2320..2751)
                     /locus_tag="LR726_000086"
     CDS             complement(2320..2751)
                     /locus_tag="LR726_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6215204.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            3100..3273
                     /locus_tag="LR726_000087"
     CDS             3100..3273
                     /locus_tag="LR726_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215205.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(3339..3947)
                     /locus_tag="LR726_000088"
     CDS             complement(3339..3947)
                     /locus_tag="LR726_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBL6215206.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(3953..4981)
                     /gene="vanB"
                     /locus_tag="LR726_000089"
     CDS             complement(3953..4981)
                     /gene="vanB"
                     /locus_tag="LR726_000089"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBL6215207.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            complement(4974..5945)
                     /locus_tag="LR726_000090"
     CDS             complement(4974..5945)
                     /locus_tag="LR726_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBL6215208.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(5942..6769)
                     /gene="vanW"
                     /locus_tag="LR726_000091"
     CDS             complement(5942..6769)
                     /gene="vanW"
                     /locus_tag="LR726_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HBL6215209.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMYQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(6787..7593)
                     /locus_tag="LR726_000092"
     CDS             complement(6787..7593)
                     /locus_tag="LR726_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBL6215210.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(7764..9107)
                     /gene="vanS"
                     /locus_tag="LR726_000093"
     CDS             complement(7764..9107)
                     /gene="vanS"
                     /locus_tag="LR726_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL6215211.1"
                     /translation="MERKGVFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(9110..9769)
                     /gene="vanR"
                     /locus_tag="LR726_000094"
     CDS             complement(9110..9769)
                     /gene="vanR"
                     /locus_tag="LR726_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBL6215212.1"
                     /translation="MSIRILLVEDDDHVCNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(10387..10533)
                     /locus_tag="LR726_000095"
     CDS             complement(10387..10533)
                     /locus_tag="LR726_000095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215213.1"
                     /translation="MTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVSRHWITAG
                     LMRL"
     gene            complement(10712..10897)
                     /locus_tag="LR726_000096"
     CDS             complement(10712..10897)
                     /locus_tag="LR726_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL6215214.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            11042..11413
                     /locus_tag="LR726_000097"
     CDS             11042..11413
                     /locus_tag="LR726_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6215215.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            11671..12000
                     /gene="mobC"
                     /locus_tag="LR726_000098"
     CDS             11671..12000
                     /gene="mobC"
                     /locus_tag="LR726_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL6215216.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            11961..13289
                     /locus_tag="LR726_000099"
     CDS             11961..13289
                     /locus_tag="LR726_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL6215217.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            13271..13657
                     /locus_tag="LR726_000100"
     CDS             13271..13657
                     /locus_tag="LR726_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBL6215218.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            13840..14076
                     /locus_tag="LR726_000101"
     CDS             13840..14076
                     /locus_tag="LR726_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215219.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(14376..14579)
                     /locus_tag="LR726_000102"
     CDS             complement(14376..14579)
                     /locus_tag="LR726_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBL6215220.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(14583..15527)
                     /locus_tag="LR726_000103"
     CDS             complement(14583..15527)
                     /locus_tag="LR726_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215221.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(15528..19448)
                     /locus_tag="LR726_000104"
     CDS             complement(15528..19448)
                     /locus_tag="LR726_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBL6215222.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(19597..21678)
                     /locus_tag="LR726_000105"
     CDS             complement(19597..21678)
                     /locus_tag="LR726_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBL6215223.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(21675..22904)
                     /locus_tag="LR726_000106"
     CDS             complement(21675..22904)
                     /locus_tag="LR726_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBL6215224.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(22894..23145)
                     /locus_tag="LR726_000107"
     CDS             complement(22894..23145)
                     /locus_tag="LR726_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBL6215225.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(23168..25159)
                     /locus_tag="LR726_000108"
     CDS             complement(23168..25159)
                     /locus_tag="LR726_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL6215226.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(25191..27590)
                     /locus_tag="LR726_000109"
     CDS             complement(25191..27590)
                     /locus_tag="LR726_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBL6215227.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(27520..27942)
                     /locus_tag="LR726_000110"
     CDS             complement(27520..27942)
                     /locus_tag="LR726_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBL6215228.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(27956..28492)
                     /locus_tag="LR726_000111"
     CDS             complement(27956..28492)
                     /locus_tag="LR726_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBL6215229.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(28529..29392)
                     /locus_tag="LR726_000112"
     CDS             complement(28529..29392)
                     /locus_tag="LR726_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215230.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(29484..29699)
                     /locus_tag="LR726_000113"
     CDS             complement(29484..29699)
                     /locus_tag="LR726_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBL6215231.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(29776..31584)
                     /locus_tag="LR726_000114"
     CDS             complement(29776..31584)
                     /locus_tag="LR726_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL6215232.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(31581..32057)
                     /locus_tag="LR726_000115"
     CDS             complement(31581..32057)
                     /locus_tag="LR726_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBL6215233.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(32132..32344)
                     /locus_tag="LR726_000116"
     CDS             complement(32132..32344)
                     /locus_tag="LR726_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL6215234.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(32366..33331)
                     /locus_tag="LR726_000117"
     CDS             complement(32366..33331)
                     /locus_tag="LR726_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215235.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(33377..33565)
                     /locus_tag="LR726_000118"
                     /pseudo
     CDS             complement(33377..33565)
                     /locus_tag="LR726_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(33915..34607)
                     /locus_tag="LR726_000119"
     CDS             complement(33915..34607)
                     /locus_tag="LR726_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL6215236.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVSYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            34602..34868
                     /locus_tag="LR726_000120"
     CDS             34602..34868
                     /locus_tag="LR726_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6215237.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(35044..35613)
                     /locus_tag="LR726_000121"
     CDS             complement(35044..35613)
                     /locus_tag="LR726_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL6215238.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(35687..36976)
                     /locus_tag="LR726_000122"
     CDS             complement(35687..36976)
                     /locus_tag="LR726_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215239.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            37330..38178
                     /locus_tag="LR726_000123"
     CDS             37330..38178
                     /locus_tag="LR726_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL6215240.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            38192..39265
                     /locus_tag="LR726_000124"
     CDS             38192..39265
                     /locus_tag="LR726_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL6215241.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            39255..41426
                     /locus_tag="LR726_000125"
     CDS             39255..41426
                     /locus_tag="LR726_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBL6215242.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(41538..43709)
                     /gene="copB"
                     /locus_tag="LR726_000126"
     CDS             complement(41538..43709)
                     /gene="copB"
                     /locus_tag="LR726_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL6215243.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(43729..45915)
                     /locus_tag="LR726_000127"
     CDS             complement(43729..45915)
                     /locus_tag="LR726_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL6215244.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(45915..46124)
                     /gene="copZ"
                     /locus_tag="LR726_000128"
     CDS             complement(45915..46124)
                     /gene="copZ"
                     /locus_tag="LR726_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL6215245.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(46137..46577)
                     /locus_tag="LR726_000129"
     CDS             complement(46137..46577)
                     /locus_tag="LR726_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL6215246.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(46651..47124)
                     /locus_tag="LR726_000130"
     CDS             complement(46651..47124)
                     /locus_tag="LR726_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HBL6215247.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(47434..48900)
                     /locus_tag="LR726_000131"
     CDS             complement(47434..48900)
                     /locus_tag="LR726_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6215248.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(49211..51295)
                     /gene="cadA"
                     /locus_tag="LR726_000132"
     CDS             complement(49211..51295)
                     /gene="cadA"
                     /locus_tag="LR726_000132"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL6215249.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(51819..52178)
                     /locus_tag="LR726_000133"
     CDS             complement(51819..52178)
                     /locus_tag="LR726_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL6215250.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(52208..52549)
                     /locus_tag="LR726_000134"
     CDS             complement(52208..52549)
                     /locus_tag="LR726_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215251.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(52546..53220)
                     /locus_tag="LR726_000135"
     CDS             complement(52546..53220)
                     /locus_tag="LR726_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL6215252.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(54015..55313)
                     /gene="asnS"
                     /locus_tag="LR726_000136"
     CDS             complement(54015..55313)
                     /gene="asnS"
                     /locus_tag="LR726_000136"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL6215253.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(55353..56543)
                     /locus_tag="LR726_000137"
     CDS             complement(55353..56543)
                     /locus_tag="LR726_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL6215254.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(56564..57091)
                     /locus_tag="LR726_000138"
     CDS             complement(56564..57091)
                     /locus_tag="LR726_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL6215255.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(57138..59927)
                     /locus_tag="LR726_000139"
     CDS             complement(57138..59927)
                     /locus_tag="LR726_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL6215256.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(60074..60274)
                     /locus_tag="LR726_000140"
     CDS             complement(60074..60274)
                     /locus_tag="LR726_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL6215257.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(60726..61046)
                     /locus_tag="LR726_000141"
     CDS             complement(60726..61046)
                     /locus_tag="LR726_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215258.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT        15056 a        14991 c        13461 g        17678 t
ORIGIN      
        1 attgaaaatt atatgggatt ttagattatc tttttagaag ttcttgaatg tcggtatagc
       61 ccgtgtttcc gggcttttcc ggcatttttg atattgttag atacttacaa atgttcttat
      121 tacctctcac catttccccc tcaaaagtag taaatggagt agtaaaaccg gccttgtgtc
      181 aggctgccag ccgttccatt tctgcccttg cggaatcgaa tgtggcgtgt gcataatagt
      241 tcagcgtcat ggtgatattg gaatgtccca taatgtactg caaggctttg gggttcattc
      301 ccgcattggc taaccgggtg cagaatgtat gacgcatagc atggggcgtc gtcgttttcg
      361 gcaacgcttc ttcatggctc ttgttgtact tcttcacaag cctgccaaac atcccgccat
      421 aatctaccgc cgtcatggga tagccgttct gtttgagaaa caggaaattg gcatacccgt
      481 caataatgaa cggctttgca cctttgcggt tttttatcac tcgctggaac gcctgataca
      541 cttcctcatt catggggatt tttctgacgc cgctttgggt cttgggttcg tcaatatagt
      601 aaccgacgcc ggtatttctc aaaagctggt ggctcacgat gattacccgg ttttcaaaat
      661 ccaaatcttt gtccgtcagt ccgcacagtt ccgaaatgcg aagccctgtc cccagcagaa
      721 taacgattgc gtcataatgc ttttggtaaa ccttatcacc ttgaataaag gacagaaaac
      781 tttcttcctg tgctggtgta agaggcacct ttggttccgt atcgtcgtcc agaacgtcac
      841 taagctggaa gtcaaacggg ttttttcgga tgcaatcgtc ctgtatggcg gtgtaaaaag
      901 ccgctttcag ggaacgcttg tcgttattga tggtcttata agaaaggccc ttttctttca
      961 tccgaagcgc ccattccttt gcgtcggaca gcttcacact gtcaatcggg cagctcccca
     1021 gccgatcttg ttccagaata cgcatcagcc gttcccggcc cagtttggtg ttgtgcttca
     1081 cattgccccg gtgcctgatc tgcttggcgt agagctggca gacggtcatt ttcttgccga
     1141 tggtgtctat cccgtcgtca aggtctttct ggatttcttt ctccttttcc cggagtgaaa
     1201 tatcctcccg tttacctgcc ggggtcttat ccgtaggcac taacttccat gagtaaacaa
     1261 actgcggttt tccaaaggta tcaacatatt tgtaagcata tcgtccgtct tttctctggc
     1321 tctctccaga ccgcaaaacg cgatttttgc tgtctcgtct tttttctgac atctcgtcat
     1381 gctcctttcc atgacggaaa gagccgcata tatgcttata aattattata acatatctgc
     1441 ggcccttttt cactattaga ttgtgtccaa agtgtcaatt attttctcaa actgcttgcg
     1501 tttgatctgg atgcggttgc cattcataat gacccagcca gaggccggat tttcctcggc
     1561 cagcttacgc aatttgtttt ctccgatacg gaaatattta gacgcttcct caatcgtcag
     1621 cgtgtatttt tcccaaatag gcacatcatg gttcataatg cagccccctt tcacaaattc
     1681 aaaggtttgt gtgatttagg ggcatagacg gacggctgct ggcacagctc cacgggactt
     1741 tcacccccgc gtggttctca cgcggcccta cccattgcct gcgacgcttt taacgctcgg
     1801 actgtggcta taagggagta tcatagtcct gcctgtacgt cgtcgcccgc aagccgccac
     1861 gctcacattg cggcgtggtg ttcctcgctc cgcttttccc gtagggaaaa gtatcatggc
     1921 gcaccagccg tccggctcgg cacagacaga atgtgtccgt atgccctatg atgcgccgcc
     1981 gttatcttgc gggcatattt atcaaggtgc gatcaggcca ccgggaaaac accgaaacgg
     2041 ggaaagggta agccgtttca tgctgccggg gccggttcgt cagttcgtga cgatagcgtg
     2101 gatgagcttg atttccagac ggcgcttcat ggcctcgtcc agacacacat gcggatagcc
     2161 gttttcgtca tagagcgtcc gggtacacag cttgttcatg taaccgctgt aatactgcaa
     2221 aacgcgctct acggctccgg catccccagc gcgggcggcc tcgatcaccg aaaggggcaa
     2281 aagctcaatg cccttgtagt tcgggttctt catccgcgtc taacctccct tcggcatcaa
     2341 cgcctttagc tgtttccgca gctccgataa ggtatttgtc ctgtgccgct gaacggcact
     2401 ccgggacatt cccacaaggc cgccgatctc accatctgcc atatccagaa cacagtgcag
     2461 gattaaaata ctctgctcca tgctgggcag cgcggcaaag gcttcggcaa caagctcatt
     2521 gttgatatgt aggtcatagc catgcgacga aaacgtaaag ctgtcgctgg ggtagtgatc
     2581 tactgtacag agcttgtcca aatctgcctg tgacaaagag cttagtgaca cttcacggtc
     2641 acggcggcgc ttcatttcag ccaagtagct tttggcctcg ttacgcagta ccgtcttgca
     2701 aaaagcatcg aacatgcacc gcgcttcata gtcgcggggg atagcatcca tcttctcacc
     2761 ccctttctgt gggggactta ggtggttctc tccctttccg ctaacaagac tttgacaagg
     2821 ccgccagcta cccgctaaat acctcctttt tgaaaatttc tcaaaaaatt tttttgggga
     2881 cagccgggcc gcaaaagacg acaaaaatcg cccggcaggt cgcaaaccta ccgggcgaga
     2941 aaatgctttg ctatgctgct gatttacatg gtatagtaca tactcataac cggaatagcc
     3001 ctgtggaggg gcaaggcttt ttttgagtgg tggaagtgga ttagagcatg gcaatagcaa
     3061 aacagcatag cggcatcctc ctatgggccg ccacaccttt tgctgccaga aaagaacggc
     3121 ggccttgatt tctcaaaacc gccgccagca aaatacaaaa tgggcgtggg aaaggtctgc
     3181 ccaacagcgg tttttttctt ttctgccgct gggcagattg aatactataa tttgcaatgt
     3241 attacaaaag atgctttaac cactttccag taaaagattg tccatgacca gcatagattt
     3301 tatcaaatcc aaaatctgat acaaaaccaa tactttcatt atgaaacggc aaaattaaaa
     3361 taggtatcgg ggtatggctc atcaatcaac ttatagtgcc accattcaaa gcgataagac
     3421 tgaaatccgc tgctttccat gattttacgc aagcatcttc gattttgtgc ctctgcatcc
     3481 cctatccctt ttgctgtatg atgcgaccgt tcgtccataa aatcgaagtt acttcccatt
     3541 gaaacaagtt cccctgtatc caggtggtag agcgtaagat caattgcgct tccacggcta
     3601 tggctggatt gtgaggccac ataacccttt gtaatcaact cggctcgctc tatattgggg
     3661 taaaattttt cttttgtgag cttgtcctcc ggttgcgctg cccaacgcag gaagcagtcc
     3721 accgcagttc ttggccgata tccatcccat aaaagcaagc catagccaag cgcagccgca
     3781 tggatttgtg cctcgcgcag agcaaacgcc acggcctttg tgccgatgat gcgattcacc
     3841 tcatacccat ccaccggttt tcctgtgaag ttatcccatg tggcgtactt ggcatcccaa
     3901 cggacgccat gcaacatttc atctaaaaac aaaaaaccat tttccatact tatcacctct
     3961 ttaacgccaa tgtaatcagg ctgtcaatca gtgcaggaag cgtgattcct gcggcggcca
     4021 ccatacgtgg gtagcggctg tacgatgtaa aaccgggcat ggtattgacc tcatttagaa
     4081 cgatgccgcc atcctcctgc aaaaaaagat caacacgggc aagccctctg catccaagca
     4141 cccgatatac tttctttgcc gtttcctgca cccgatttcg ttcctcgacc ggaatgtctg
     4201 cgggaactgt aatcatcgca ttttctgagc ctttttccgg ctcgttttcc tgatggatgc
     4261 ggaagatacc gtggctcagc cggatttgat ccacttcgcc gacaatcaaa tcatcctcgt
     4321 tccccatgac cgcacacccg acctcacagc ccgaaatcgc ttgctcaatt aagatttttc
     4381 catcatattg tcctgccgct tctatcgcag cgttaagttc ttccgtaccg tttactttgg
     4441 ttacgccaaa ggacgaacct gaccgtgccg gcttcacaaa gacagggtag gtaagcgcac
     4501 ccgcctccgg cttgtcacct ttatcaatca tttgaaattc gggaacggcg atgcccgcat
     4561 tttttgtaag aatgtaggcc agtgatttgt ccatgcaagc tgcggagctt tgaatatcac
     4621 agcccacata ggggatacca gacaatacaa acagcccctg tatcgcacca tcctccccgc
     4681 atttgccatg caaaaccggg aaagccacat caatacgccg tgtttcgtat tcgctttctt
     4741 tcatgacaag cagcccatgc gttttcctat ccggggagag tatggcgggg agactgtcgg
     4801 cttcccattc cgtacatggc ttcttgcata gcttccatac accgtttttt gtaattccga
     4861 tgtagtgcgg atcgaatttt tccgtatcaa tgttcgcagc aatttctatt gcggatttta
     4921 ccgacacatc atgttcctcc gagcaaccgc cgaagatgat tgcgactttt attctattca
     4981 tgctgtaaac tcctttcaaa attgagacaa ttcctgattg ttttttctgt agtatcctgc
     5041 agcacacgct cagtgtagta cgccgtatgg ggtgtgatga tcacattcgg catccttaaa
     5101 agctgcgaca aaaagggatg gtcaagcact ttctgcgagc aatcggtata aacaaactga
     5161 tcctcgccct ccaacacatc cagtgccgca ccacccagct ttccgctttc caacgcctcc
     5221 accagcgccc cggtatcgac aagcgctccg cgcccggtgt tgattaaaaa tgcgccttgc
     5281 ttcatctctc caatttgctt ctgaccgatc agatggcggg tatccgcaca aagcggcaca
     5341 tggagcgtaa caatatcgct gttttttaga agctcatcaa gctggacata atctgcatcc
     5401 atttttcggc tgttatcata ggccagcaca tggcatccaa atccccgcag ccttttgacg
     5461 accgcttgcc cgatatggcc ggttccaata actcccacag tcatatcccc cagttctttc
     5521 ccccggatac gatccaatct gaaatcttgt tgcgccacgg cgtgcatggt gggttttgca
     5581 ccccgtatgg ccatcagcat cagcatcaaa gcataatccg caacgctgtc cggcgaatac
     5641 gcaaccgtgc caaccgagat tcccattctc tcggcggcag tcgtatcaat gtgatcgcag
     5701 ccgatgctgc gggtagaaat gtattttacc ccgacctttc tcagcgcaag aattgtcgcc
     5761 tcggaaacct cggacttatg gcctacgcta acgcattgat tgccagcggc caattttgcg
     5821 ttgtctgccg atatcgcatc gctaatcagc gtagggataa tatgaaaatc cggcgataaa
     5881 gcgcggaaag catttgcctc atcctgttcg catccaaaaa cagtaatgcc cacacttttt
     5941 ctcattggtt ctcctcctga atatccacac tttccgggag gggataacag attttgcatt
     6001 tgtttgtata aagcagcttg caatctatta tttccccggt acctaaggca acttgcatcc
     6061 gtttaacctg ggcatattca taaatcccgc cactttcacg gacatactgg atactgccat
     6121 ttgtaatttt gtataatgct tggggctctt tgtcggcgga cacctgaccg atgattttct
     6181 catcatctaa ggtcacccat atttggtagg tgcagtcggt atcgtttcgc acttttaaat
     6241 caatccagcc ctctgaaatg gttgcatcca cccctttaat ctcgtcactg tttggctctg
     6301 gaaactcctt tacttcgtga cctctgcgct ggataattgt caatggcgta tgcaggaaca
     6361 tccaaaatag taaattgctc atctggtaca taccgccgcc cgacatggtg gtgagtttac
     6421 cattggtcac cgtaaggccg tctttatagg gggtatcttt gtccgcatgg cgtaccagcc
     6481 accagaaaga aaaggtttcc cccggtctta tcaataagcc gtttaaggtc tttgctgcca
     6541 gctttaagtt gaacaccttg ttttcctggt atatcatgtc aaatccggta ttgtggttgt
     6601 ataatgcgca atccgtttca aagagcaaat gggaaagcga tttttcccct atcgtctgcg
     6661 catagcgaca gccgtcaaat ctcattcccg cataaaagca catttttctc tgcgcccgtc
     6721 tcattggaag caggaacggg aaacgctgtg tcaatctttt tctgtccata acataacatc
     6781 cttttctcat ttttctggtt ttaaatattc ctcaaggcaa accccttggt tgtacatctc
     6841 tgtggcagct tcgatgccaa catatcggta atgccacggc tcgttgctca caccggttat
     6901 ctctgtcttg tctggtgggt agcgacgaat aaaaccaaaa cgatagctgt tttcatccag
     6961 ccatctataa acctcgttgc ctgttgaatg aattccgtcc gcattgatat ccacagcaag
     7021 accaagctga tgctcgctcg ttcccggcac ggccacccaa gtttctgctt ccgctttagc
     7081 ctgtgcagag gtgtagcctt tcgccttata ttcggcaatt ttttcatcca taatttcttg
     7141 ctgtttttct gttgttcggt atccggatgc gacaatcggg taaactccat cagttcttgc
     7201 ggcatcaaac aaatcttgaa gatagggaga aatccgaata tctatccgct caccatttga
     7261 tagttgctca agctccacat cgtactgtgc ggggatgggg ttctgcctgt tcactaaaat
     7321 caggctccat tcctgttcat ctccctgttc tttggcggtg gcaagagcgt tttcctccaa
     7381 attctccact acgtcagtgc tgctgcctgt atgagttgca gggatgggcg gtatctgtgt
     7441 tgcctccaat acggaagcca gtctccaccc caaaaacagc gtgcagactg tgaacgaaat
     7501 aatgaacgca catagaaaag cccgcttttc cacggattgt ttcatatgcc gtttgtgatg
     7561 atccgcattg gaatgatagt tgcttttttc catttgatcg aatgcctcct ttgcaataaa
     7621 actacgattt gtggctggta cgattgttat tatggcaagc gcatctgtac tttccctgta
     7681 gccagtctgt agttttcctg tagcagcaaa aaaagaccgg cagggttata cctgtcggtc
     7741 aaaatcattt aagttttaaa tatttacaat gttgatgtga gcggtaaatc cagccagaac
     7801 aaaacgccat ccgaggtgtt ttccagcgca tactggaggc tcatggcatc cagcgttttt
     7861 tgtacgatgg caagtcctaa accgcttcgc ccactttttc tgcttcgcgc ctgatcaatg
     7921 cgatagaatg gggtgaacag ccttggaagc gcagtatcat caatgtgaac gcccatgttc
     7981 aaaacaaaaa ggcggcattt ttcagcacca ggctcactcc atatccgtac ctcgcctccc
     8041 tgcggcgtgt tctgaactgc attcaatatg acattggata gtgccttttg gagcagcctc
     8101 ggatcggaca ggacaatttg cccggctgga atatctgtga cgaaccgctg gttgtttgcc
     8161 tctgccaagg tttgaaaatc gggcagcaac tcggcaaccg tgcgccctat gtccaacggt
     8221 tcagctatgg gtacgattct cccatcattc aggctgacaa gctccagtat ttcggaaatg
     8281 attttgccct gcctatccat cattttgatg cattcgcgca gatacttaga atgatctttg
     8341 tagtcaccga tattttcaag cattccctcc aacagaacgc ttgtagccgc gatgggcgtt
     8401 tttagctcat gagaagctgc cgcaaagaaa tatcgctgtg tttcctccaa ctcatgttcc
     8461 cttgcgattt catcctccag ccttgcgatg gtttctttca gcctgacata catggaatgc
     8521 atatcatgag ccagtgcgcc aagctcatcc tttcgctcca gcggcggcgg tacatctttc
     8581 aggtttgcca ttttattcgc actgtccgct aaggctttga tcggcgttgt catttgccgc
     8641 gcaaagatat acgcgcataa aaggctgaat acaaccatta tcgcaatcat aacaattccc
     8701 cgaattgtca gcccttgata aagcaatccc acacctgcct tgctttgagc aacaatcgaa
     8761 atattatcat ctctatgtac cacataaaga aagtcgggtc taaaactatt tgatgtattg
     8821 gcatttggtg tggcatagag tacgcttccc tctttatctt caatataaaa ctcaaaggat
     8881 tggttattgt agtgaaacag ccctgccacc tcttgaatat caagcctatc gctattctga
     8941 atcagttcca ccaatggctg ataggatttt actgtttgct gtaattccat cactctgaaa
     9001 taagacacaa attgctgtgc aaacagtgtt gctgttacac cgacaagcag tagcaggaca
     9061 atgatcgtat aggaaaaaac cttaatgaaa accccttttc tttccattat tatgattcct
     9121 ccaatcggta tcctacaccg cgaatggttt tgatgatatt ttccggcagc ttggcccgca
     9181 aatttttgat atgagtgtgg actgtgctgc catcaccctc aaagtcatat ccccatatac
     9241 gtgacaaaat gatttcatgc gttaaggttc tgcctttgtt ctgcaccagc agcaaaagga
     9301 tttcaaattc ttttcgtgtc aggggcagct ctgtaccgtc acaaagtacc gtgaaatcct
     9361 ccggcagaag tgtcagcctg ccgacacgga tttcctttgc cagcgcaccg ctgcgccgca
     9421 gcagggcttc cacccgcttt agtaaaatct gcatcttgaa tggctttgtt acatagtcgt
     9481 ctgcctctgc atcaaacgcc cggatttggt tttcgtcatc cgacagggct gtcatcatca
     9541 gaatgggagt atcatttttc gcacgaaatt cacgcaaaag ttcatgcccg ttcataccgg
     9601 gcagcataat atcaagaata accagttgat aagtgttttc gtaaaacttg gtgtatgcct
     9661 catttccatc tgtgcaggca tccacctgat atcctgcctc agccagaaac gcccttactg
     9721 tattacagac atgatcatca tcctcgacaa gtagaattcg tatcgacata aaaatccctc
     9781 cattttaaga agataacata acagtctgta gtttccctgt agtttcaaag cgatttcttt
     9841 tcggatttta atcatccgtc attgggatac tcgcttcttt tcatggtgta tgtatctata
     9901 ttattggttt aaacgaatta aatcaagata ataataatta acaccttctt aatgtttggg
     9961 atgtttcgat tacagcggtt gtcaaaacat cccgataata ctggcaagca atggatcaga
    10021 atatccttta cggatattct gatccaccac ttgcccgcct gcggcgttct cgttttgctt
    10081 gttggggaca ccccaagcct cagcaaaatg cgcggcctct tttttgcgaa tgcaccttgc
    10141 ggtatggctt ttcacacagg cagggcctgt gtgtactccc tccggtcgct cttgccgcac
    10201 tccgcgcagc agattcagtc ctaataccgt tcaagacagt agaaaatcag caagtctata
    10261 ttcctcatat cttcatcatc cgtgtccttg cagttacagg aaatgtaata atcgccaaat
    10321 gtaaatccca gaatattttc ctcaaaaagc cgtacctccc cggtattaaa atcaatcacc
    10381 ccatactcaa aggcgcatta gaccagcagt tatccaatgt ctggagactt caccataaag
    10441 aatccagatg ccggcctttt caattctacc atatacggct gatacagcag cagcttcctc
    10501 gtctttgttt cgggcatttt ccatttctgt catgataaag tttcgcttct tcatacatcc
    10561 atgtgtaaat cttttctttc tgtttcagat gagaatactt tttatcggtc tggagcagcc
    10621 tgccatctat catcttatag tttttccgtg acaaaatacc atgctttctc tctcttcaaa
    10681 accatatcca aaatgcgccg gtttcattat actattcagt aaccgccttt cctttctttg
    10741 tacgcctgtc aatcggcttt tccgcagttt tgggaatgag ccagatcgtc gccatcttta
    10801 cagcgccggg gatgcgtccc gcagcacaat agtagtttat ccatcgtggg ggcacacccc
    10861 atttctcgcc tgcctctttc aacgtcatgt agtccatagt acacctccac agggtacatt
    10921 ataattcttt tgctcgaaga ataaaagaaa caaaatgtga aatataaact ttaagaaagg
    10981 tgcatatacc aacattccac gggcagagta tgaaatatac aatgtaagtg ggaggtgcga
    11041 tatggcgaat attgaagatt gtcccggctt tgaaactttc ggcgcggatg tgaaagcggc
    11101 ccggcaagca aagcgcgttt cgcgcaaagc aatggcagag aaaataaata ttgactggcg
    11161 ctatcttgcc aacatcgaaa acgacggggc cattcccagt ctgccggttg tcatacagct
    11221 tattaaagcc tgcgggcttc ctgtggaacg ctactttaac ccggagatca tgcgggagga
    11281 aagcgaactg cggcaaaggg tcagccacaa gctgaaactc tgccccgaag aatacctgcc
    11341 gatcatcgag ggcgcgattg acagcgccat actaattgaa aagccgaaga agaatgaaac
    11401 ggaggatgtg taactggcat cctctgtttt acttttagcg ggagctgcac agcgtagctt
    11461 ctattttttt gcactttttt ctttccagag ggctattttg cgggtagttt ggggctttgg
    11521 aaacgtcttg ttagtggaga aacagtaacg tagcaagcat agggagtgta gccactcccg
    11581 gaaaaacggc ctgccccgtc cctgccgggc cgcgtccgtt ttgcttgttg gggaaatccc
    11641 caaacccctg aatggaaagg acggtgaagc atggcaaaca gaaagcgaaa aatcgtgctg
    11701 cgcgtccctg tgacgccgga ggaacgggct atgattgaac agaaaatggc gctgctgcat
    11761 acgaagaact tttcagccta cgcccggaag atgctcattg acggctatat cgtcaacatg
    11821 gacaccagcg acatccgggc gcagaccgcc gagatacaga agatcggcgt caacgtcaat
    11881 cagattgcaa agaggctgaa cggtatgggg ccggtgtacg cacaggacat cgaggatata
    11941 aagggggcgc tggcgcagat atggcagtta caaagataca tcctatcaag tcaacgctga
    12001 aaaaagccct tgactacatc gagaacccgg acaaaactga cggcaagctg ctggtggcct
    12061 cctttgcctg ctcttatgaa acagcggaca ttgaatttga aatgctgctg gcgcaggcat
    12121 accaaaaggg caataatctg ggccaccacc tgatacaatc ctttgccccc ggcgaagcca
    12181 cgccggaaca ggcccatgag atcgggaagc agcttgccga ggaagtgctg caagggaaat
    12241 atccctatgt gctgactacc catattgata agggccacgt ccacaatcac attattttct
    12301 gtgccgtgga tatggtaaat cagcgcaagt acatttccaa caaacagagc tatgcctata
    12361 tccggcgcac cagcgacagg ctgtgcaagg aaaacggatt gtccgtggtg aagcccggcc
    12421 agagtaaggg caagagctat gccgagtggg acgcccagcg gaaagggacg agctggaaag
    12481 cgaagctcaa agcagccatt gacgccgcta tcccgcaggc aaaagatttt gatgatttcc
    12541 tgcggctcat gcaggcgcag ggctatgaga tcaagccggg aaagtttatt tctttccgcg
    12601 ctcccggaca ggaacgcttt acccgctgca agacattggg ggaagcctac accgaggaag
    12661 ccatcaaaga gcgtatcaaa ggccgcgtta tcaccagagc gcccaaagag cgcaagggga
    12721 tttctttgcg gattgatctg gaaaacaaca ttaaggccca gcagtcggca ggctatgaga
    12781 ggtgggcgaa gctccacaat ttgaagcagg ccgcaaagac tttgaatttc ctgactgaac
    12841 atgggataga cacctacccg gatttggaaa gcaaagtggc cgagatcacc gccgccagcg
    12901 acgaggccgc cgctaccctc aaagctatgg aacaccggct tgcggatatg gcggtgctga
    12961 taaagaatat ttctacctac aagcagctct ggcccgtggc tatggaatac cgcaatgcag
    13021 cggataaggc aaaattccgg cgtgagcatg aaagcacgtt gattttgtat gaagctgcgg
    13081 caaaggccct aaagggacag ggtgtgaaaa agctccccga tctgtacgcc ctgaaagccg
    13141 agtacaagcg attggccgag gaaaaggaac ggctgtatga gaagtacggc gaagcgaaaa
    13201 agcagatgca ggaatacggc atcatcaagc agaatgtgga cggcattttg cgtatgacgc
    13261 cgggaaagga atggacgcaa gagctatgag tattccacga atgaattacc ctcaatatcg
    13321 caaggcccgg aagctgacgc atgagtgctg caactattgt gacggcaact gcctgttgct
    13381 ggacgacggc gaagaatgta tttgcgtaca gagcatttcc tattcgcttc tgtgccgctg
    13441 gtttaaggcc gctgtcctcc cgctggatgc ggccctgtat gccgagatca ccaaagaccg
    13501 ggacgaagtg aaacgctgct gtgagtgcgg ggccacgttc acgccgaagt ctaaccgggc
    13561 caaatactgc gcggcctgct ccaaacggat gcgccgccgc aaagaggccg agcgccagcg
    13621 gaaaagatac tggaaaccta cgcacttaga gctgtaaaag cccttatatt tcaaggcttt
    13681 tccggcatgg tcagcggggt atgtgggtgt tttatcctat ccccgccgaa gtgctgttct
    13741 aaatgcgtag aatttaccag cagaaaagcg ccgttttctc tggttccatg cttccgaata
    13801 tggtatcata gaaaaaatca gtctgacggg ggtatcagga tggacggaca agaaaagctg
    13861 ctggactatg aaacgatcaa agccgccgtt gccggtgaga ggtgggccac ggaaaaagtg
    13921 ttggcccatt atgctgacta catcaacgag ctttccacgg tggagatcag acaggcaaac
    13981 ggcaaaaaga agaaagtcat tgacgaagat atacggcagc gcatttccct gaaactgctg
    14041 gaagcattgc cgagcttccc gctggaacag agatagacgg caagagggct gcaccctctt
    14101 acgccgcttt gcggctatca cctgcacacc cctgaaacag atacacccgc tgcgcggcta
    14161 tatctgtttc aggggtctaa ttatagctgc aacctacgca tttaagggat tgaaaaccgc
    14221 ccgctgcaag gcttctcccg catgtttgca ggggcaagcg aatacttcat cctatccccg
    14281 acaataccgg gttctaaatg cgtaggaata ggcaaaaaga aaagacgccg gacgcaagcg
    14341 accacatggc cgccgcgccc ggcgtctgct gttcgttatt cgtctgtgaa gtggaacttc
    14401 tggccgtcat agtcggcatc cgtcatgcgt tccagcttgg aaagggtctg ctgggcctgc
    14461 gcccgcatat ccccatccat aaccggcatg acggcggtga gcctgtttat cgttctgcgc
    14521 cggtctgcat tgtggtacat acagatcagg ttttcttcct ccacggtaaa gtgcatcatt
    14581 ccttatacct ccaattcatt ctttttggtt ctggccgccg ccttttttgg gctggcggcc
    14641 tgtttgctgt cggatagctg cttgcggata gagggccggg gcctgcgaat actcacatcc
    14701 cggcgctcgt cccggttaat cacggcatac acgccatgcc tgcctttcat cccggtaaaa
    14761 cacagggatt tatagggcag catggcaaag agccggtcat tatccttggt ggaagcaatc
    14821 gccgcaaagt gcggggagat ctccaccatg aaatgcgttt tattggggct gttaggcgcg
    14881 gacagatctt tcaggctgtc cacaatacgc cgggcctcgg tttcaatccg gctgtcatgc
    14941 aaagcggtga cgtggacttt gaaatcgccg gggacggccc gcccccggct gtgctgctcc
    15001 gtccgtagcg cctcctgcag cacagccggg tcatggggct gggcctcata gtacaaaaag
    15061 tttcccaata cccggtcagg gcttccgtca aaacgctggc tctccgggat gtcacgggtg
    15121 ccatgctcat aaaagagccg gttcacactc accggctggg cggtttcaat gacgtgccgg
    15181 aaatgctgcg gatagtccag ctcatagaga ttgccccgga tagtgccgcc ctgtgtgccg
    15241 gtcagctcca ccgcgtaggc cagtatcttg tcacgggtct gctccccgta aaatttccat
    15301 gtgttgtact gtcgggtgtc tttcaggaaa acatcacgtt cccggaaaca gtacgtcccg
    15361 gagggccggg acatccagag cagggtcttg tctgcggcct gatcgctggc ggcatcccgc
    15421 ctgataatgt ctttgtcaat gccaaagtca ctttgataaa acgcggtgtt ctgccgcatg
    15481 atctgttcca gcgcggccag aacgtccaca ttttcaaact tctgcattta ctctacctcc
    15541 aatccctttg atttttcccg cgccggtggc tgcttggcct gttcggtctt ggccgcgttg
    15601 agctgcgccc ggatagaggg cttttctttc tccctgcggg ccgcaagctg taaggcgctg
    15661 ggcttttcgc cggaaagggg ctggatgcac ttgatacggt cagcgacgcg gattgtggtg
    15721 tcgtttaact gccgctgcca tgccccggcc ttgggcgacc agcggaaacc gttgcttttc
    15781 agctcgtcgc gtacagcagc atccggcttc ccctcaaaga agatttgcag gcggttggcc
    15841 acccggttga tctccacagt gccgccgtca aactcccatc ccacataggc cgcctccttt
    15901 tggcgggtca attcctcaat gcggcccttt aaccggcgta tctcggcgtt attgttggag
    15961 agctgccagg actggaacgg ttttttctca tagtgccagc ccgacgccat atccgctttc
    16021 aggttttcaa tgcctctttc ggtcagatgg gggcatccgt ccagcgtccc atgcttgcgg
    16081 tagtaggcat tgacggcctt catcaactcc tgcgacgctt ccagcttttc cagtttcttt
    16141 tccagcttgg aaacggcctg cggatcgtcg gcgctgatac cacccatgcc ggtgctgcgg
    16201 attttgtcaa gcaagccctg aatgtactga aattcctcca tgttgcggtc actggcggcg
    16261 ttctgctttt ctttcgcccg taccgggaaa ttggacggcc cggcaatcag gacagagggg
    16321 acgcgggcgg taatctcata gcccttattc atgttctcgg ccagcttgcg ggcgtaggtg
    16381 tcaaggaggc tgtctatctt ttcgtgatac tctggctcca cacgcttttt ctggcgctcc
    16441 gcaatttcca cggcctgatc gacatagtgc cggtattccg ccgtggcgct gccgggccgg
    16501 tagtcagaaa agctcatggc ttccttggcg cggcgggcgg ctccctcatt gatgggataa
    16561 tagcggacag ccgtttgcgg gggcgcagcc tccgggggtt cctcctgtgt ctggccggaa
    16621 gcaactgcca catcctcaaa agatagctgt tcgggttcct ccggctgcgg ttcgggcggc
    16681 gtattgttga taatgccgtc aatcatgttt tcgttctgct ccgtagacag ttcggcggta
    16741 cgcaggggat tttcccgcaa aaactccggc acttccatat agccgaagct gtctacatag
    16801 tgggcggtgt cctgtccatt ctggtgcagc accaccacgt cggaaacgga aaggctgtgt
    16861 cccctaaaat ctgccgggtg gtcgatgttg aatgtgcggt aaatatcctc caaagacgtg
    16921 cttttatcca gcggtgccgt ataaacaagg tcatagttgg cactgtccac ggccagccct
    16981 gcggcctgta aacgctcgta tggctcaaac cgatagtcgc gggtttcctc cccgcctcga
    17041 agctggtaga tggaaaaggt gtcctgttgg ggtgcttcat agccagccgc cgcgtattcc
    17101 tccacggtca taccggctgc ggcggcctcc tgcgcgatct cggcctgtac ctgtgctttg
    17161 tagtcctgca agctctggtc gaagtcagcc agtttcggcg gctccggcaa ctgatactgg
    17221 ccctgattga gcatggggcg gcactcctgc acataagaga gaagcgcccc caaataggcc
    17281 gtttcctccg gcgtcagggt ttccccggac tgtaagcggc cctgcgccgt ctgttccatt
    17341 tgggcaaggc tgcaatgcag cttcatatag gggacaaact cgcctaacag catatcccga
    17401 tgggccttgt cctgctccca gccctctatg ccggttgtag cgattacatg ctttttccag
    17461 ttttcgtcct gcgcgtaaaa ttggtggtag ctctggatat gttcgatcag ggaaccgtcc
    17521 ctatctccaa aatcctgccg cccctcgtag ctgtccggct cgccctgaaa gatacagtca
    17581 atgcggaacg cggttttgtc atagcctgcc tctgccggat atgctgcatc cagacgggca
    17641 aacagcgcgt cggcctcatg caacggcatt tgctggccgt cctttagctg ggggctttcg
    17701 ctccatagca cagtcacaag cggctcggct gccggatcaa gggccggggc ctgttcttcc
    17761 tcggcctgcg ccggggccgc tgcctccggg gtaacgtcaa ccgtgacggc ctcctgttcc
    17821 tgccgggcct cgcgctcctg ctgaagctgg gcaaaatggc cgtcgatctc ggttatcaat
    17881 tcgctggccg tggcccggat ggtttcaagg gaggctttca gctcggataa ctccctgccg
    17941 ctgctccaat cggccacata gccaaaagaa tagtccgacg tgtccagccc gtaatactgg
    18001 cagacggtat aggccacgct ttcggcctgt acctcgcggg tgctgcggtc tggccgggcc
    18061 gcctgttcct cggcgggggc ctcccggtca atggcgtgga gcttggcatg ggcaatctca
    18121 tggatggcgg tctttagggt ctgcaattcg ctcatgccct cgttgatggc aatgcgctgt
    18181 tttgcgtaga agcagcggcc cttggccccg tctgaaatcg gctcaatgct tatgggatag
    18241 ggtgatgtcc gttccagcgc ggcaaagaaa tcctgatact gctgcacgtc gccggtaagt
    18301 tcactgatat agggcatggg aaaatccttg ccctcggtct ggctgatgtc aaagactgtc
    18361 accaccttaa aggccggaac cgtcacttcc tgttcttcgg tcacgggctt gccgtccgcg
    18421 ccgatcacgg gctgctgggt ggccgggtcg attttctcca tctgctgctt aaccttgaac
    18481 ggggccgggg ccagtatctt aatgcctttc tcgccccgct tcacatggcg gtcaaattca
    18541 tcctgccacc tgttatagcc cgccacaatc gaaccgccct gcatggcgat cagcagcgta
    18601 ttgttcagac tgtaatcgtg catcttggac atagcggata agtaagccat atagcggcta
    18661 ctttcaaaaa gctcctgtac gccggtttcc agacggtcag taatcacttt catgcgttct
    18721 ttgctgttca tgccgtccag catgatcggg ataaccgggg gcggcgcttt gtcggggctt
    18781 tcggatgcct ccggctgctg ggcctgcggt tcctcccggt cagcttccgg ctgggggtag
    18841 tgcatcaccc ggtattcctc cggcaccggc tggccctcat ggacgcgctg ccattcgtcg
    18901 ccgcttctca ccagatagcc gtactgggta aaaacgcctt gttcctgctg ggcaatgtgg
    18961 cgtccaaact gcgccgtatc aatcccggct ttccattcct ccggcatatc caccatgccg
    19021 gaacggttca ggtagtagtc gcccaactgt ccatcccccc taacgtcggg aagatgcaca
    19081 tagtaatcca cattttcggt gtagtcgatg atctggccga tattctcaaa gccgccacgg
    19141 ggattttgca gggcggcgtt cagctctgcg atctcggcgg cgtcaagctg gcccagccgg
    19201 gcggcaagga aattcagctc gtccacgtcg gcggccagca ccatgtcata gggaatggca
    19261 aggtgtctgt cctccgggca ggaatacccg ttgataaaaa aatcctgcgg attgtcggca
    19321 gtaatgccaa catcccgcag ggcggcttgc agcgtttcgg ttgtggtggg caaatccagc
    19381 cagacgcctt gtttgccctg ttccaaaaac tgccggttat ggacttggat agaaaatact
    19441 tcgttcacgc ggtcactcct ttctctgttc tgtttcaaaa aatagggggt tggggacttc
    19501 cccaacaagc aaaaatcccc ggcccggcag ggacgcggcc gggggatttt accgggagtg
    19561 tagccacact ccctatgctt gctgtgttac tgtcctttag ctcctgtgtt caatgcggta
    19621 gttgacgtac ttgcccccgg tatcagccaa aagcaccgtc ccgtcgtagg ttttgccggt
    19681 ctttacggaa tagaggccct ttaccttgac tttgccggtt ttgagcagcg cagcggcgat
    19741 cttcggggtg aacgctttct tgcgttcctc aaaaaatcgg tcattcttcc acatgacgaa
    19801 ctggcaggcc cggtttccgc agtagtagtt tttcttgccc tcatagacag cctcaccaca
    19861 gcggggacac gtcccgatag gaacacgctc ggcctgaaac aatttctgct tttcctcact
    19921 gatacaggaa taggtctgca ccagctcccg cgcctgcgtc tcgatgccgt ccataaattc
    19981 gtcaggatcg gcctgcccct tggcgatctc ggtcagccgg ttttcccatt cggcagtaag
    20041 ctggggagaa gtaagcgcct ccggcagcac cacagccaga ttgatgccgt ccttggtggg
    20101 aatgagctgc ttgccttttc tctggacaaa gcccatctgc accagctttt ccagaatggc
    20161 ggcgcgggtg gccggtgtgc ctaatccctt gcgctcggca tcctccggca tatcctcggc
    20221 cccggcccgt tccatagcgg acaaaagcgt atcttcggtg tagggcttgg gcggcgtggt
    20281 gaaatgctcg gtgacagagg cggccacgtc tgcaaaggtc tggccctcgc aaagctccgg
    20341 cagctcccgc accgcctcgg cgtcctcgtc ggcgtcggtc tttaaggaag aacggaaccg
    20401 gcgttcaatc tctttccagc cgggggaaag gacggtcttg cccttggcgg taaactcctg
    20461 cccggcacag gtaaatgtgg cggtgacggc ctcatagata tgcggcccgg ccacggcaca
    20521 taagagcttg cagcagatca gggacagcag ctttttttca ctctcggcca gagtgccaaa
    20581 gccctgcttc acaaattcag cggtggggat aatggcgtgg tggtcggaca cctttttgct
    20641 gttcaggatg cggtcaaact gcggggaaag gttcaggccc ttggcaaacg gcaccagctc
    20701 ccacaggccg gacacaaggc tttccacggc gggcagcata tcgtcggtaa gatattgact
    20761 atccgttctc ggataggtca gcagcttctt tagatacagt gcctgggcat agtcaagggt
    20821 ctgcttggcg gtatagccga aaagccggtt agcctcccgc tgcaaggtgg taaggtcata
    20881 gagcttgggc ggctgttccg tctttttctc ccgcgtcacg gaaacgcaaa cggcctgcgc
    20941 ttttgcacaa gccgccttga cggtgtttgc ctcggcagcg tcggaaaagc ggctgcttac
    21001 cgcgtccgcg ccgcccgccg tgatatggac gatgtgatat ttctctttct tgaaatcggt
    21061 aatcttggcg tccctgtcca ccagcatttt taaggtcggc gtctggacgc ggcccacagt
    21121 caggttcttg tggtacagga cggaaaaaag ccgggtggcg ttaatgccga tcagccagtc
    21181 ggccttggcc cggcacaggg ccgattgata cagcggatca tagtcgccgc ccggtttcag
    21241 gtgggcaaag ccgtccttaa tagcactgtc ctccatagag gaaatccaaa ggcgctcaaa
    21301 gggcttttgg catcccgcca tctggtagac gaaacggaaa atcaattccc cctcgcgccc
    21361 ggcgtcggtg gcgcacacaa ggccggtcac gtcggggcgc tgcatcagcg aacagagaac
    21421 gtcaaactgc tgtttcttgt cgtcggggac gatgtactgc catgtttccg ggagaatggg
    21481 caaatcctca tagcgccatt ttttgaaacg ctcgtcatag cgtccggcgt cggcaaagga
    21541 caccagatgt ccgatgcacc agctcaccag atagccgttc ccctccaaat agccgtcgtg
    21601 tttctccgtg gccccgatca cgcccgcaat gctgcgggcc acgctgggtt tttccgcgat
    21661 tacaagttta tatgtcattc ctcgtcctcc tgttcactca caaaatcagc ttccgggtca
    21721 aactcggttt cgggttcctc gtcctccaaa tcctcgtcct ccccaaaatc atactcgccc
    21781 aaatcgtcgc tgccggtggt ttcagccttg ggctttttga acttgaaata gtaaagggcc
    21841 gcaccgccgc ccgccagcgc caccagcagg aacacgatca ggccgcccgc gccgcccttg
    21901 ggttcttcct ccggggcttc gggttcctcc ggctggggtt cctgcggctc cgggccgctg
    21961 cactcggtca ggttggtttt acataccggg caattcgtgt tcacagcccc ggccttgcat
    22021 ttcgtggtac aggagcagac agcagcgggg gtgacggttt ccccgtcctc catcagcgtc
    22081 agcagatcgg cttcatccac ctgatttaag aaatggacag tttcttttcc ctcgtcgtcc
    22141 cggtcaataa tcagataaaa cacattgccg tttttggttt ccacggtgat aaactgcttg
    22201 ccatttttcg cggcgctgcc gatgtcgtca atcagtgaca ggttcccgtc cggggacagg
    22261 gcggcgctgg ccccgccgcc attgaacagt tccagcagag aagcaatttc ctccgggtca
    22321 atggagaggg aaaaaccgcc ctcgccggta gccgctactt ccttttcgct gacggtcata
    22381 tagtcggaat ggacatagcc gatgcgctcc ggcaagagaa ttttaatcca gtcgccgtct
    22441 gtgccgacta cctcaaccgt cgtcccattg ggaagctggg tgaacgcggt cttgtcaagc
    22501 cccgcgccgg tgcggacatt cagcctgccg ccgttggtgg tgacggtgcc ggtctgttca
    22561 ggttcttttt tgtcggtgcc ggtggtgatc tggatgccgt cgtccgtggc ggcaatgctc
    22621 accttggaac cgaacagcgc ggaaaacagg tcggtgatgt ccttgccggt cagagggccg
    22681 gtatcttttt cggcctcggt ttctgtcccg tccgtggcgg tgccggtttc gtctgtttca
    22741 ggctccaaac cgcccgtagc gggttcggtt gtagcctccg gcagcggggc cggtgccggg
    22801 tcattgccgc ctgcaagggc ggtaacggga aaggccgtga cgcagagcat cacggccaga
    22861 agtgcgggga gaatacggga aatccgtctg gtcttattct tcatgggcat tgtcctcctg
    22921 ttcatggtaa ggggttgcgg caggggcggc ctgcattgcg ccgctgcgta agaacgcggc
    22981 aaattcctcc ggcttcatgt tcatgccgcg cacaagctgg atgatctgca aattctcctg
    23041 ttcggttttc tgcgtttcca gctcccgaag ctgtttctga aactccgcga tcttagactt
    23101 ggttttctgg atgtcctttt cgatgcggtc aagtttggtt gccataattg aaagctcctt
    23161 tctggcgtta aggtaaacgc ccgtaactgt aaaaatggga ttgccagtag tttgaagtaa
    23221 ggttcgtgta ggaaatcggg ttcccgcaat gcagcatcac attgttgccc acatacaggc
    23281 cgacgtggga cacgccgggg gtgtcgtagg ttcccttgaa aaatacaagg tcgccgggct
    23341 tggcctgcga actggaaaca ggggtgcaga tgttatatag gccctgcgcc cccagccgtc
    23401 ccacgttcca tccactatga ttgattaccc acgaaatgaa gccgctgcaa tcaaagctgg
    23461 tgctgggaga gctgccgccc cacacatagg gataaccgat atatttttcc gcctctttga
    23521 gcatggcggc aaaggtggca tcctccaaag cctccggggg tacgtcgtag taggtcggcg
    23581 gcgtcagggt ggaggcgttg gggtactggc tgccggaaaa cagatcaggc cggtttccta
    23641 gcgtggacat atacagggaa tagaaactta gcgtttcctc gtccatgata tagactggca
    23701 catgggacag gtcgaagttt tccagcgtga cgtagcagat gtaatagtca tacggcactt
    23761 ggtaggtgtc tgtatgggtg ttgccctccg cgtccgtcca tgtatcggtt tcggtgcggt
    23821 aacgggtttc taccacgacg ttttctgtca ggatatattg cttgtcaaag agcatggaca
    23881 aatcaccctg tacctccccg atggtaaata tcccgtcatg gagcgccgac agaatggaaa
    23941 tcagcacata ggggtcatgg ctgatctcgt caaggtcaaa atggtattcg tcatagtcat
    24001 gggtggcctc gtagttgtcc agatagtctt gcagctcggc ttccagctcc gcgtaggcgt
    24061 cctcggcggc caacatatcg ctatcctcgg aaaagtaaga ggacgccgtg atgccgcttc
    24121 cggcgctgcc gaacatggcg gtgcaggact gtaagccgcc gatgataagc agcatcaaaa
    24181 gccccaacac aaggacggcc agcgcccctt tccagtggcg ggccacaaag gaggccgccc
    24241 gttcactttc tttggctgcg gccttggccg ctttcgtgga ggccccggct gtcttggccg
    24301 cgccctgtgc cgccgcgcct gcggtctggc ccgctgtccg ggcggccttg gcgtactggc
    24361 gcttgatctg ctgtttctgc cagaaccggg aaatggggtt gctggccgcc tgtgcgatct
    24421 ccgggttatc ccgcaaagat ttctggtaga aatactccgc gttggcagac aatagcctgc
    24481 gctcggcctt ttccgcgtcc cagtagggtt tcagcctgtg gtgccggatg gtgctgcgga
    24541 tggcttttcc ggcctgccgc tcggcaagtt cctccccctt atggccgccc tccacaccca
    24601 cgttttcatg ctcgacttca tggattttcc cgtggccgaa tagaaaaacc tcggaaacag
    24661 gacggcctgc cggattgggt ttcagggagg gaggcccctt gtcctgtttg tcaaagtgca
    24721 ggacggtctt tgctttgccg cttgcctcgt caaagacggt ttccttgacc gggacgcgct
    24781 tcttggggat ggcggcgcgg gccttatcca gcttatcggc ccgtttgtcc gattttcgga
    24841 tatagggctg taaatccggg tcggcccgtt cttcctccga cagttggagc cgggcggatg
    24901 gccggtgcag ggcagcgtcc ccctcggcgg ccacagcccc ggcctgcttc gcggctttct
    24961 tggccttatg gccgtcatgg atggcgtcgg cccgttccag cagcttatcg gcggtgccgc
    25021 ccggccccgg cgcggacaaa tcctgttcgg cctcccggct ggaaatgttc tccacctcgc
    25081 cggtggcctg attttccagc gtcagcccgt cgcgggtcat ggcctgcgtc actttcgcct
    25141 tggctttcag ctctttcatg gtggttcacc tcctttgtgg cgtcgccggg tcatactccc
    25201 gccacctcgt ttaagcgggt cgtcataatc ttgtaaagct gggtgttgtg cgggatgggg
    25261 ttggtaaagg gcagcaccac gttttcaaag atcaaaaggc cctcgccggg ttcggaattg
    25321 tcgatgtact tctgctgttc ggtggacaaa ttcaagcgtt ccgcaaggat ttttctatcc
    25381 ccggacgcct gattgagcag cgtaatgaag tcgctgtttt caaggatatt ttcaatttcc
    25441 ggggaggaaa gcaggtcttt gacgttctgg gtggcacctg ttggaatgcc gccccatttc
    25501 ctaaaccgct tccaaatctc cgcagaatag gccgccgtct gttcctcttt caaaagcaaa
    25561 tggaactcgt cggcaaaata ccatgtggcc tttccttggc ttcggttggc cgtgacgcgc
    25621 ccccatatct ggtcttggac aatgagcatc cccagctttt tgagctgctt gcccagctcc
    25681 ttaatgtcaa aggacacaag ccggttgccg atgtccacgt tggtacggtg gttaaatacg
    25741 ttcagggagc cggacacata caggtcaagg gcctgcgcca cccggtcagc ctccgggaca
    25801 tgctgatcta acaacgcttt gtggaggtcg gacaggatcg gcatattctc cgggcggggg
    25861 tctgccagat agggccggta gatcacgcgc acagcccggt ctatcacggt cttttcaatg
    25921 gcttccagcc cgttcttgcc gcccatgaca agctcacaga acgacagcac aaagtcggat
    25981 ttcagggcca gcgggttatc gtcctccgaa taattcaggt tgatgtccag cgggttcaca
    26041 aagtccttgc tggtaggcga cagccggatt acctgtccgt ggagccgttt gacaaggggg
    26101 taatattcgg cctccgggtc tgaaataaaa atatcgtcag gcgtcgttaa gaacacactg
    26161 acgatctcgg atttacagct catggatttg ccgctgccgg gggtgccgag cttcaaggca
    26221 ttggggcaac gggcctgctt cctgtccagc ataatcatgt tgcgggactt ggcgttgatg
    26281 ccgtaataca tggccgcgcc gccctgaaaa agctcctgcg tcacaaaggg gacgaacacg
    26341 gccacgctgg aagtcgtaag gctgcgctgt atcttgatct gattgacgcc cagcggcagg
    26401 ctgcttgcaa ggccctgttc ctgctggtag tccagccgga caaggggaca gttatgcttt
    26461 tgggccacgc ctgcggcttg gaacacgtcg ttattcagct tttgcttggt gtcggccacg
    26521 ttgaggacaa ggaacgtcaa ctggaacagc cgttcattgc gggtctgcaa ctttgtcagc
    26581 agcttcttgg cgtcctcgcc gtaggtggca aggtcgctgg gaagaatgtc catatcgtaa
    26641 ccgctgcgga cggccttttt ctgctccgca atcttcatag cgtcaaggtc ggtaattttg
    26701 cgcttgatgg tcttgatcgc cttggtctgt tcaatggcct gcacatggag attgactaaa
    26761 acaccgcttt ccgtattgag gaagtcggcc agcatatcgt ccgacagctc cggggacagg
    26821 atttgcagga aacttaccgc gccgtatttg ccgcccatgc cgaatgtctt tgcattgccg
    26881 aagcacaggg aggacggggc aataaaatcc ttggtggaga ggccggacgg ggccagccac
    26941 ttccagtcaa aattgaatat ctcgccgtcc gggtggtaca cgccatgcag caggttcagc
    27001 cgttcccagc cgtccatcac atgggctacg gcccccatgc tcttgaaata gcctaacagg
    27061 tcaaagccga tgcgcttcaa tctggctcgc gccgctttca cgctttcggc ctcgatggtg
    27121 aatgtcaggt atttggtctt taccatgccg ttgttgccgt ttgcaagctg cttttgcaga
    27181 atgccggtgt actcggcccg gatgtcgtcg aagtcgtccc cctgcggcgc gatctcaaag
    27241 ctcttggcgt actgcaccgg gtcaactttc cgattgagga aagaaaactg gatatgaatg
    27301 gaggcgtcca catagttata caggtcgcac agtttttcaa agatagccgt ctgcgtgtcc
    27361 ggctgggcaa gctgatagct gatgtccgca aactcaatgc acttggaaaa gacgccgggc
    27421 gatacccggc acacgccgtc cgggtacatg acctcgtagg ggatgctctg ctgcgccgtg
    27481 tggggcctgc cgtcgccctt atatttacgg attaccgcgt caatttcttt tcgctgggcg
    27541 cgggtcagct ttgttttttc ggtttctctt gggatttcct ttttggacaa tggcctttac
    27601 ctcctgttcc atctggtatt gccgcacaag ggcggtgtag gtgttgtcgg tctgataggg
    27661 acgctccttt ggctggatga agcagcactg gatgatctgc ataatcacca cttcaagggg
    27721 ctggccgtga cgctcgtaca tggcaagcaa aaagcccggc agcatggcaa ataccatgag
    27781 cagggccgcc gcgctgttgg taagatagtc acgggtgaga aaatataagg ggacgccgat
    27841 caccagcgcc ccggaaaaac agatcagttg ccgtttggta agcccaaaca caaccttgct
    27901 tttcacatgg gttaagtcct tggggacggt tacataagcc atttggcacc tcctttcacg
    27961 tcaggataag gtcacattcc acctcgttcc cccacacgtc ccagccgggg gttttctgtc
    28021 tggcaaacag ctccacgcgg ggcaaatcgc ccgccagctc tacgattttt tcacgggtca
    28081 catccggctt tttgctgtgt tgttcaatcg ggctgataat aaactgatgg acagaggcag
    28141 aacgacgctt cggcttgccg tgtgttgcca gcaggcagat ttccgcgttt ccccttgtcc
    28201 agcggcccag cccgtaaaac catgtaaggc ttttacggtt ctgtttcagc catacaaaag
    28261 ccacggtctt gaatgaaaag ccccacgctt tgattaaccg cagagcttcc ggcagcatgg
    28321 ggaatgtggc ccacaaaaac agcaggcagt ctttggacgc cagccgttcc accggcaggg
    28381 cgcacagctc gtcaatgctc atggtggaat agtgattttc cgctgcgccc tgtacgtttt
    28441 tgcagtcata ccgccacggc gggtcagcgt aaatgatgtt gtacgttgaa ataaacaagt
    28501 tcctcctgtg tatgcttgga ttttgggatc aatgcgcccc gaatacggcc tttgatatac
    28561 tgccggtctt gaacaggcag aagcacagca gcaccgtata gcccatacag ccccagattg
    28621 cgccggatat gtcggaagcg gaaccgatat tctgtatcag caccgcgtag atacccacca
    28681 caaccatgat taaaaaggct tggaagccca gcgccagcag ggatttcaga tacccttggc
    28741 ccatgccgcg ccattccgcg ttgcccattg tggcaagggg gataggcccc agcgacgtga
    28801 caacatagat ttccaccatg cggccatagg tcacaaggaa aatgcagatg gaaaggatat
    28861 tcatggtgag gcccacaaca agggtttgga accacagccc caaaagagcg cccacgctca
    28921 tagcctccaa ttggctttca aggtctggta tcagggtgtt aaaatcaatg gatgtctgcc
    28981 cgataatcac accggcgctt tgattgacga cgctttgggt ggcgtcgaat acgcccatga
    29041 cgatgttcca tgtgttcgtt acgatcagga ttgcaaacgc ggatttgaat atccagcgga
    29101 agaacatgga gctgtcaaag tcgtgcatat tgttcttttc cgttatcatc tggataagct
    29161 catagcacat gacaagggcg agtatggccc cggctatggg gacgatcacg gtttccgaca
    29221 ggctgtgcag catattgaaa ataccgctgt tccatgcctg cggggttcgt cccacgtcgg
    29281 cggcgatttc tccgactttg gcatttaccg tatcgaacag cccggacagg ttgcccgtga
    29341 taccgtccat gaggatgcct tttatccatt cttcaatgat ttcactaatc acgggtagca
    29401 cctcctttcc cgcgccctcc cggtctggcc gggaggacgc ggaggcgatt agttaagggt
    29461 ttggacaggg acaaaggcca ggatcagccc agcagcccgg acagcagggg aaccagagtg
    29521 acaccgatca gggccacgcc gccgccagcg acaagctgct tgacaccctg cgattttgcc
    29581 cccggattgt cctgcccgta tccctccaac agattgatgc cgccccatac gcacagagcg
    29641 ccgcccagtc cgatcacgat agtctgcaaa gtagtaactg cggaattaaa aaatgccata
    29701 aatttacctc tttctgccgg tttccggctc ataatgattt tttgattttg ttgttggaat
    29761 gaaaaaagcc gctgtttatt cagcggcggc gtcctcgtcc gacaggtcga tttcatacag
    29821 gtcgaatact tcatcctgcg ttaccacttg ctgccgtttc cggcgcgtga tagtcaaaaa
    29881 tctgtccacg tcaaaggtat tcttcttgtc ggcgtcggca aggtatttgt accggggatg
    29941 cttggtaata tcgaatttct ccgataaaaa cgggcgcaca ccccgtagct gcaagataca
    30001 cttgccgccg tccatcacgg caatttcgtc ttgggacatc aactcctttc ctaatttttg
    30061 atagttcaat ccgtgggacg tttggcttcc ccggttctcg ctggtgttga aactgtcaat
    30121 cgtttctttc cccagcgcat cggaaatctc cttggcattt ttgccgcgcc cactaaggaa
    30181 aagcacacta tcgcagttat ccgaaatgat ctcggcggcg tccttgtaaa tcgcttttag
    30241 ctgcgactgg ctctgcaaaa taatagaggc cgatatttca cggcttcgaa tggtggcaat
    30301 gagtttatcg aaattgggta tttggccaat gttggcaaat tcatccaaaa tcaggcgtac
    30361 atggacaggc agccgcccgc cgtacacatc atcagcccgg tcacaaagca ggttgattag
    30421 ctggctttgg agcatagcca gaatgaaatt aaaggtcgtg tccgtatcgc tcataatgag
    30481 atacagcacc gtcttttcgt ccccgatggt gtccagctcc atttcgtcgt cctccatcag
    30541 ctcgcgtacc tcccggatgt caaagggcgc aagtctggca ccgcatgaaa tcaggatgga
    30601 agaacgggtc ttgcctgcgg acagcaggaa tttcttgtac tggcgcaccg caaaatggtc
    30661 ggggtctttc tgctccaaac gctcaaacat gagatcgacg gggctttgaa actccgggtc
    30721 gtcctcgcgg gcttcactgg cgtttatcat ttccagcagc gttgagaagt tcatttcctc
    30781 cgctggggcc tcgtaccaga tatagccgat cagggccgaa taaaacaacc gttccgactt
    30841 cacccaaaaa tcctccgctg acttttcccc ctcgcccttg gtgttggaaa tcagggtatt
    30901 taccagcttt agcacgtcct tttccgaatg gatgtagaca aaggggttgt acttcatgga
    30961 cttggaaaag ttaatggtat tcaggatttt aatgcggtag cccgcctgct ggaacatcct
    31021 gccggtttcc agaaccaaag tgcctttcgg atctgtcacc acaaacgaag tcggatagtc
    31081 cgtggtcgga aagcattgca tcaggttggg tttcacgaaa aatctcgttt tccctgaacc
    31141 gctgccaccg atcaccagca cgtttttatt ccgcgctgtc ttggggtctt tgggcctgcc
    31201 ggtcatggta agccgttccg tttgggtgag caggatatta ttttcaaact tggggtcgat
    31261 gtaaggcgct atgtctttgg ccgtccccca gcgggcgctg ccatactcca tgcctttgcg
    31321 gtatttcttc acgttcttgc tcttgcagta caccaccagc cgcacgatca cggcaccggc
    31381 gataccgata cacaggtcaa acaggtgcag gcttggcagc gggttggcaa aagccgccgc
    31441 aaaaccatcc gcaaaatgca ggagcttccc cgacaggtca gcaccagcgg ccagccggta
    31501 cgcctccccg catttcgcaa acaggtacac aaaaatcaga tacgggaggt tcaggatcag
    31561 caatttcttt acgtcaggct tcacagttcc agcccccttt ccttgatttt ctccttggcc
    31621 cgctgcttgg tctgggccgc cgccttttgc ttcacagcgg acaaaacctt gcggatagag
    31681 ggccgctgct ccttggaaag cgtcttggcg gtaaactccc gaaacgccag atcaaggtta
    31741 tctgcatcct tggccttgaa aatcacaacg tagtggggcg gctgggtggt cttatctttt
    31801 cgtaaggtaa aatccacgcc gtatttcttg gcgcatggct tgaacgctcc aatgttggcc
    31861 tcggtgatct caatgttgga gagatcagcc ccatgctttt taagctgctt taggctctgc
    31921 tggccgtggt gcagcgtgtt tttgcccttg gcccggtgtt gcaggtactt tttcagcgcc
    31981 gcttgcagca cttgagccgt gagcttgccc gttttcatgg acagggcaat ggtcttttga
    32041 gtaacttctt cctgcaatcg catccctcct tgtcagaaag tcaaaagcgg cgtctaaggc
    32101 ggaaccacca gacgccgcag gtagatatag gtcaaatccg cacccgcagg ccggaaaggt
    32161 cgtcggcccg gatgccgaca agataccagt tttcggccat ttcaatgcgg gtgggcctcc
    32221 atttgccgcc gatcatcacg tcgaaacact ccccgcaatg caggccgccg taatagtcgg
    32281 ccagatcaaa gcggatgtcg taacggtctg ttcgctcgtc aaaaatcaat gcgccctgct
    32341 tcatagggtc gtcctccttt tctcgttaca ggctttcgcc ctcgccgcag gaatagtccg
    32401 gcaggccgga tttaggcttt ccccaatcgg gctgtgccat gtcgtgcgcg acaagggccg
    32461 tgtagtagtt ggaaatggtg ctgggggcgt tgaacagcat agcccgcagg tattgtttga
    32521 tattgcggac tttcgttgtg ttctcccgca gacagtcaag gacaaagcgg atatgttcgc
    32581 cggtcagctt catgtacttg gatttcacca gctcggcagg gtagtcgtcc ccggcgacac
    32641 ggattgtctt tcgggcggtg cagacggttt ccagcatcag gtctacgatt tcgtcaagct
    32701 gttcacggtc aatgctcctg tcctgaatga gataggcata ctcgatgttc tccaaaatga
    32761 tttcccgata aatccgtaca gcctcatcct ttcctgtcct tttccgttcc ggcggcgtag
    32821 ccgctgcctc gccctccata ggggcagggg tcggggatgg gataggaatg gaatgggtac
    32881 tttgtccatc tgtaattatt ttttcttttt ttgataagtc agttctttgt acttctttat
    32941 ttaattgcgt tggattttcc gacgccggat tatccgatgt cggattttcc aacaccgggt
    33001 tatccgatgt tggattttcc aataccgggt tttccaatac cggctgttcg tagatggtat
    33061 agaccatatc ggccattttg ccttttgcgt cacggccctg ctcacgccgg atatatccag
    33121 ctttttcaag ctcccatacc gctgtcctga tcgcgtcgat gctttcccgg ttgatatagg
    33181 acagtccttt tagggtatag tcccaatcct ccggcaagga caacatctgc gacagcaggc
    33241 ccttggcctt taaggtcagg ttcttattcc gcaggtggtg atttgacatc accgtatagc
    33301 ctttgttctt ttccacacgg aaaactgcca tagcgttgtt cgctcctttc actgtgaatt
    33361 tgaccgctgc atagtcggtc agcttgcccg gctgataggg acactctgca aactggcaga
    33421 attgatattt ccaatccggg cggtaaaatc ggcaggtgcc gcaatcctcc ggcgcgtccg
    33481 ccccgccgcc gtcgtagtga tcgaaaccgg gcttttcctg catcagccgt tcaaacgccc
    33541 ggtcagggcc ggaagtaaat agcatggcgt cgcctccttt ctctatttgg gcataaaaaa
    33601 gcggcgtcct gttctcccat gacgggaaag aggaacgccg cgtttacggt gtcgtattca
    33661 atttatgatg tgccgtattt ggggtggcgg gtattagcag tatggttcag catatatgcg
    33721 gctcaatccg aaagtagtaa attggtagta aattgagttc tcatatcctg cgaaatgccc
    33781 gtatttccgg ggctttcagc gataacctaa aattttcatt gaaaaacaaa tgtatatttc
    33841 cgcaaaaaaa gcaccccttt taattagatt tctagtctaa cttttggggt gcacatcaaa
    33901 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
    33961 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
    34021 ttcttattag ggaaatcacg catattgaaa gatacatgat agctaacatt tcctaaagta
    34081 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
    34141 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
    34201 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
    34261 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
    34321 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
    34381 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
    34441 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
    34501 aatggaatac catatttcat tgctgaatct cttgccaatt caacgtatac tttatagttc
    34561 ttttcaaaag tttctttatc atgtgtgtgt tgtaactcga tttgcacagg acttttggct
    34621 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
    34681 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
    34741 attttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
    34801 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
    34861 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
    34921 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
    34981 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
    35041 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
    35101 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
    35161 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
    35221 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
    35281 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
    35341 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
    35401 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
    35461 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
    35521 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
    35581 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
    35641 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
    35701 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
    35761 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcggtgatc actagatctg
    35821 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
    35881 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
    35941 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
    36001 gtaaaagcat cccccactct actgcggggt gtgcaaatgc ttgttggatc gctagccgaa
    36061 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
    36121 cttcttctat cagccggtca tggataccaa ttacgtaacg aagccattct tggtaaagta
    36181 cttttgtcag ctttgatttg ttttcgctct caaattcgct attcagccaa aacagcgaat
    36241 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
    36301 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
    36361 ttattggcaa aatctgctct tctctaaata gcaattcagg aaacaattca aagaatagtg
    36421 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
    36481 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
    36541 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
    36601 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
    36661 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
    36721 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
    36781 gaacggtttt cttatgaaga atcagctgga tgggacagtc gctttccgcc aaacgtttca
    36841 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
    36901 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
    36961 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
    37021 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatttt
    37081 cctcattatt tttaatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
    37141 taaaaaaaca taataaaatc actttgcttg taggatgtat tcccttccat tacaatgaaa
    37201 taaatagcaa ttttatgaga aggtgatgtg aaaatggaag aaaaaatcga agaatccaaa
    37261 caaaaaatcg atcagttgat acaagaatta gaaaaagcgg tcatcggcaa acgttcaact
    37321 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
    37381 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
    37441 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
    37501 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttggca
    37561 gatgagatca atcgcacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
    37621 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
    37681 acgcaaaatc caatcgagta tgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
    37741 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctactttta
    37801 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
    37861 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
    37921 cagttagtgt cagcaacaag aagccatgct gccatccagc ttggcatcag tccacgagga
    37981 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
    38041 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
    38101 gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc atactcttgt tgcacaaatt
    38161 cctgttcctg tgaggtagat tgaggtagat aatggaaaaa atcaaagatt atttcattaa
    38221 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
    38281 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
    38341 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
    38401 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
    38461 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aaacaagaga ttttgattct
    38521 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
    38581 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
    38641 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
    38701 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
    38761 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
    38821 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
    38881 tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa cctctgctag acttgtattt
    38941 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
    39001 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
    39061 agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
    39121 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
    39181 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
    39241 aggaggtgaa tggattgacg attaaatggg aaaaatggct gtgtggcgtt tactcctttg
    39301 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
    39361 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
    39421 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttgtat tttccttcga
    39481 agacattttc cttattatgg atcttacagt ttttttcgtc agtcacagag gaattcttcc
    39541 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
    39601 tgctgatcta tttatttatt acattgaccg tggattacga ctatttgtac gcaccctttc
    39661 tttctttgat ggcttacttc atgatgctta ccgtttttcg ggaaaaagat ttactgtacg
    39721 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
    39781 tgcggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
    39841 tcttaactat tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
    39901 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgat acactccatg
    39961 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
    40021 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
    40081 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
    40141 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatggaag
    40201 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
    40261 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
    40321 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
    40381 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
    40441 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
    40501 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc gaacagtatt
    40561 taaaaacaag cggaaatttc cgctattcca aaacggatac cggacatact ccgaaagaac
    40621 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
    40681 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
    40741 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
    40801 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
    40861 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
    40921 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
    40981 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
    41041 tattcagcct tggggtgtgt ttatacgtct tgttgttctt tctattgtta ttcagaaaat
    41101 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
    41161 catatcaaaa acttcttaac catttggaaa cattggtacc gcgcaaaccc gcagaatctt
    41221 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
    41281 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aatttcgata
    41341 aaaaaaccta tttagaaatt acgcatataa taagaaaaac ggtatacaaa aaagttctga
    41401 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
    41461 cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag gttgtgacac gttttttgtc
    41521 acaacctttt ttcattatta tttgattttc aatgtcaaag catttagagc tacaactacc
    41581 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
    41641 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
    41701 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
    41761 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
    41821 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
    41881 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
    41941 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
    42001 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
    42061 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
    42121 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
    42181 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg gtttttgact
    42241 gttgcctcta gcccaactcc tgaaagattt tttagatttt cggcttgata aggcttgatt
    42301 tcatgttctt tcaaataatt cataatacca acagccaatg gatgattggc attcgcttcc
    42361 aatgctccta aatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
    42421 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
    42481 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
    42541 attgatgtag agcgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
    42601 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
    42661 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
    42721 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
    42781 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
    42841 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
    42901 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
    42961 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
    43021 tcttcttttc catctttcgt caagcgtttt actgattctg gcagtaattc tgctagtttt
    43081 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
    43141 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
    43201 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
    43261 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
    43321 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
    43381 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
    43441 gctaggataa gcgatagcca aaatttttgt ttgaaatttc ccatgtgcat ggaatgatcc
    43501 atgccgccat gatcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
    43561 tctccatggt tatgtcccat gtgcatggaa tggtccatct ggctatggtc atgtttcatc
    43621 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgttca
    43681 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
    43741 gtgccgattt aagctcaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
    43801 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
    43861 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaccctag acaactcgat
    43921 tgtttgtgca atggatgcca aactgctact catcagcgtc acatcggctg tttccatcgc
    43981 gatatccgtt ccacttccca ttgccatacc aacatcagct aatgctaaag ccggtgcatc
    44041 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
    44101 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
    44161 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
    44221 tttattctgg agctgcttga ttgcttgttt ggcttctagt ttgatttgat cagccacggc
    44281 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
    44341 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
    44401 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
    44461 agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
    44521 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
    44581 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgtcc cagttttatc
    44641 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
    44701 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
    44761 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    44821 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    44881 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    44941 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg tgacttccat
    45001 gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc gtgtcatccg gattcttttc
    45061 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    45121 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    45181 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    45241 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    45301 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    45361 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    45421 taccaataca tccatatttg gtgctttcga cttgattgca tggtacgcac ctttataaaa
    45481 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    45541 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    45601 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    45661 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    45721 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    45781 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    45841 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    45901 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    45961 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    46021 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    46081 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    46141 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    46201 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    46261 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    46321 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gatgataaat gaattttttt
    46381 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    46441 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    46501 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactaat ctttactggc
    46561 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttaacttatg
    46621 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    46681 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    46741 agctacttgg atcagctgct gtatttttgg ttcattgttt ccttcaaaat gccagataaa
    46801 agataaaaat tctaagtatt tcctgtttag tttctccgaa aatgattcag gcatatctgg
    46861 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    46921 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaacgt
    46981 agaagtataa tttccttcaa cgatccatga cggattagat accataaacg attgctgttc
    47041 ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt gatttctttg atcttccttg
    47101 cgagggacga ttttcccgac ccactttgtc caatcacgat gatcttcatt cttctccctc
    47161 acttctcttt ggaatttggt tcgatcatac cacttataac gccgcaaaaa aacaagggac
    47221 tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa tggtgttcaa tcagctgacc
    47281 ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta tttttgtttg gctgttctta
    47341 ttacttgatg caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt
    47401 ctgtcacaac ctcttgtaac aagtatttct attttaaggc tgaaccactg tttctggttc
    47461 agtaatttct ttgtttttgt ctcgacgagc aattgctggc aagatcatac ctaataagat
    47521 caagacaatt ggtgtgatga tattcgtgaa taattggaac caccatgctt taggatcagc
    47581 agtataatct acttttggca ccattcctag gatacatgca aacgcagtga aggcaaagca
    47641 ccaagcacca aagataaaac cgatttttgg atttttaaca aatttatatt cagaattgaa
    47701 tttcttataa gctttattca acatcatata tgccaagaac acccataggt aacgcattgg
    47761 catgacaact gagttcaagt tcgtcaacca ttttactaat tcattcatat ctttgatacc
    47821 aaagatcggc aacaaaataa tgattcctac tagtatacct gtcagaagat aaccattcac
    47881 caatgtacct ttacgattac gtttacgtag ccagtttggt acaaattctg gatccgcatc
    47941 tgctaacaag atttttagtg gtgcatcaat tgaaaaggct aatgcggaga tttgtccaat
    48001 catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg ataccatagt aattacctaa
    48061 aatttgaaaa gcactgtatg ccccatttgc catcaagtct ttagggatat tatcgctagc
    48121 aaacagcata cccattgcga ctgagcctaa tacagcacaa atccctacca ttgctgctaa
    48181 gaagatcatc cctttaggaa attcctttgc aggatttctt gtagagttga cataagggga
    48241 tattttttcc gcccctccaa ctgcaaatac tagcatcgaa acggttgtga aataactgaa
    48301 atcaaatgtt ggaatatatg ttttcacttg attcatatgt ggtgtggcaa attccatcgt
    48361 tggattgatg aacggtgccg ttacagctaa aagaataaat aagatcgaca tgacgaacat
    48421 cgctgttccg gccagtccgc caatcacttt caaagtagaa agtccttttg ttgataaata
    48481 taaaaaggca agaaatataa taagtgaaat aactgctacc atttgtactg acatcgtatt
    48541 tacgatattt ccatttcctt ggactgccca accaccagca atcagaattg cttgaggctt
    48601 ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa gcggcatagt atgccagtcg
    48661 tttattagat gtcgtatttt cgacccatga actgacgcct cctttactat ccttgaaggc
    48721 tgatcctaat tgtccaacga tcaatgcgta tggaataaaa taaatggcca aaatcaatag
    48781 ccaagaggtg acaactgata ctccttgctg agcatagtta ttaactacat tccccatgcc
    48841 ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag cgtaattgtt tgttgttcat
    48901 tcgttctctc ctttatgata aaaatttgtt atccagatgt aatcatattc aatttttaac
    48961 aaacttacaa gacagtgtta ttttatgtgt gtattctttt atttaataca catcgttttt
    49021 tcatattttt tccatcaaaa aaataagtta ttttctttta tttcattgac cttattgaat
    49081 ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta gttattttat aatcctatct
    49141 attttcttta catttttcat taagaaaagg atataaaaaa aagaggacgt tcattcgtcc
    49201 tcttcattga tcaacgttta ttttttacat gcaagcttcg catggcattc aaaacagcta
    49261 atactgtcac cccaacatct gcaaatactg catctcccat tgatgcaaaa ccaaaagcac
    49321 ctaaggaaag taccaaaatt tttatcccaa tagcaaagac aatgttctgt ttgacgattt
    49381 tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt cgatggttga tcattcatga
    49441 tgactacatc tgctgcttca attgcagcat cgcttcccaa tccacccatt gcgattccta
    49501 catctgctct tgctagcacc ggtgcatcgt tgattccatc tccgacaaat gcggtctttt
    49561 tattgttact ttgtaagatt tcctctaacc gctgcacttt atcttctgga agcaattcgc
    49621 tatatatctt gtccacgcct acttgcttcc cgatatgatc agcaattttt ttcgagtcgc
    49681 ccgtcaacat gactgtgttt ttcacaccag cctgttttaa ttgaaccaat gcgtctacgg
    49741 catcttcttt caaggtatct gcaataacaa gataaccact gtaactttga tcgatcgcta
    49801 gataaagcaa tgtaccgatt tcttgacttg aaggaaaaga gatttcaaat ctttccatca
    49861 atttgtgatt acctactaat acttgtttgc catcaacttc agctaagatc ccttcacctg
    49921 caaattcctg gatattagct gttggtaaaa gcggacctgc gtagccttct aaaacagatt
    49981 gagcaatcgg atgtgtagaa aactgttctg cacttgcaac atattgcata aatttctttg
    50041 gatcaattcc tgttggatcg atcgtctgga tcgaaaagtc acctttcgtt aatgtgccag
    50101 ttttatcgaa taccactgtc tcagtttgtg caagcaactc caagtaattt gaacctttta
    50161 ctaatacacc gattttactt gctcctccga taccgccaaa gaaactaagt ggcacggaaa
    50221 tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag atccattcag
    50281 aaaacgccgc tctagaaaca acaagtggag gtaaaacagc taataaaacg gccaatccta
    50341 caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct ggtgcttttt
    50401 tactgctggc attttctact aattctaata atttgctgat agtagaattt tcataagatt
    50461 tttctacttg aactaaaaat ggtttatttt tattgataaa tccgcttaat actgtttcac
    50521 caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg actaatgatt
    50581 ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca agtacaaact
    50641 gaccgatcag cacgtcttca gggttcacgg tcatcaatcc ttccgccgtc tgcacgtgag
    50701 ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga ctgactgcca
    50761 atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct tctggatatt
    50821 caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca tcaaagattt
    50881 ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc agcagataaa
    50941 tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc aataaagcga
    51001 tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctttttttca tttccatgtt
    51061 cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat tccttcacta
    51121 ctttttttgt ttcttgttca atcgtagcca aatgatcttc ctctgcgtca atcgttagtt
    51181 ttccagaagt gaagtttacc gttgcttcct tgactccatt gattttctga actccttttt
    51241 cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt ttcattttaa
    51301 tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat gaatggttat
    51361 tcatatgtca aggtgtccga aagaaaaaat gaaaaacatc tgcatcggaa aatttcttcc
    51421 atacagatgt tttggcttat tctttctttt tctcttcgat cattgccact gctcttatcc
    51481 aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag cctgttgctg
    51541 gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca atccctgcaa
    51601 aatgtccttc ccagatatac gaagctccac tacgctttca aaaatataga aagagttctt
    51661 taatgaaaat tatttcaact ttccataaac agtaacccca tataaataaa agacgtaaaa
    51721 gtcacataga agaacttata caggaaaacg catagagtca ggtgaaatac atgtatccca
    51781 cctggctcta tgctttatct aacctttatt attctctttt atacaatttc tgcaccgaat
    51841 ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat ccctatgatc
    51901 gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag gcctgaaacg
    51961 atcagccaga tgatattaac gatcaatgac atgccagact ggtgataaat tacttctttg
    52021 ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat tgggatacct
    52081 atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc gattagtccg
    52141 ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct tctattactg
    52201 tttttgatta aaactgggca aaaattgatt ttattcttct ttttttagct tccagcatca
    52261 agtattctgc aaattttttt tggatttctt ccgtattttc ttttgttact aatgtgtgtt
    52321 ggattagttt tcctgatggt cgctgatcca atcgttcttc tgactgttgc acgtccgctt
    52381 gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt ttattcttat
    52441 catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca gtaatcagct
    52501 gattgatttg aggtaaatct tcttttactg cccaccttat ttccatcatt tttcactttt
    52561 ctttcgctca tttttatctc cactaaaaaa atcgactgcg acaattgcat ctatccagct
    52621 ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc ttgtatattt
    52681 cgaaaaacca taagcaatcc ctaaagcatt gaatatacca tctaaaggaa ccagcaatgc
    52741 gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt ctttacgcca
    52801 gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga tgttgcagat
    52861 ttccgctgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt gctgagcaga
    52921 tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat tgattccgat
    52981 caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc gtacttgcag
    53041 atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaattcca tcgttggata
    53101 ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat cagttgcttc
    53161 tgctcggtat cttcctgcgt ctgtaatgct atcaccgata aataatatac gttttttcat
    53221 ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta tcccataagc
    53281 cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta gttccttcaa
    53341 acaattgaat gaacgctcat caagtgtcta gttttttatg ctattttttt cttaacgtgc
    53401 cggattcccc ttgattcctt cgttgtttgg tccagagaga tcgatcacac ccaagcttcc
    53461 gtcaatcaga ccactgatga cgccatcgaa acatatattt ataggctcat ctaagacttc
    53521 tagtaaatgt ccctttacta atatctcttc tagattgctt gggatagatt ggtcataact
    53581 ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggattat tgcatgctga
    53641 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tgctagatgc
    53701 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat taccttcttt
    53761 gtaaatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
    53821 tttgctttct tctcttgtct gctggaaact tccattatcc cgtatttaaa aaaaagaact
    53881 gcgagagttc gcagttcttt tttttaaata aacgttgctc tccggccaca agtcacaata
    53941 gtcatcaaac atgaaaaact gttttttgaa attactcctt gtgactcgga gctgaacggc
    54001 aggcgcacaa cctcttatgg ataaattcgg ttcaacaaac gtgggaatgg aatagcttca
    54061 cgtacatggt cgataccagc aatccaagtg actgttcgtt caagacctag accaaaacca
    54121 gagtgcggaa cagaaccgta tttacgtaga tcaagatacc atgaatattc tttttcatcc
    54181 aaaccgtgat tacggatttg ttctagcaag aaatcatagt ccgttgcccg ttcgcttccg
    54241 ccaataattt caccgtaacc ttctggtgcg atcatatctg cacaaattac cacgtcttct
    54301 cttgttggat gtggtttcat gtagaatggc ttgatggctt ttggatagtt taggataaag
    54361 acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag gtgaaccaaa gtcttcgccc
    54421 cattcgatgt catcaaatcc atttttcttc agtaattcga ttgcatcatc gtaagtaata
    54481 cgagggaatg gcaattttgt ataagttttt aagacttctt tgtctcgtcc tagtacatct
    54541 aatgcgtatt cgcaatgatc taaaacattt tgtactaaga atgctacata ttgttcctgt
    54601 acttctaaac tttcttcttg atgagtgaaa gccatttctg gttcgatcat ccagaattcg
    54661 atcaaatgac gtcgtgtttt tgatttttca gcacggaaag tcggaccgaa tgaaaagact
    54721 tttccgaaag ccatagcagc agcttccatg tataattggc cactttgaga taaataagct
    54781 ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc cttctggtgc agaaccagtc
    54841 aagatcggcg gatcaatttt gacaaaatga tgttgattaa agaattcata cgttgcacga
    54901 atgatttcat ttctgatctg cataatcgca tgttgtcttg aagaacgcag ccataaatga
    54961 cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg tgatcggata atcgtggctc
    55021 tcgcttacca cttccacatt tgtaattccg atttcataac caaattttga acgactgtct
    55081 tcacggattt ctccagttac taacacggat gtttcttggt gtagattttt agctgtttgg
    55141 aaaacttctt ccgacacttc gctttttaca actacacctt ggaaaaaggc tgttccgtct
    55201 cttaattgta aaaatgtgat ttttccgctt gatcgcttat ttgcgaccca agcgccgatt
    55261 gttactgttt ctcccacatg gtttttcgaa tcgatgattt gaatttgttc cacgtatgac
    55321 tctcctttta tattccttga atttcttcag gattaatttt gacttttact ctcaataaat
    55381 tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat cagtagcata actcatgcgg
    55441 acattttctg gtgccccaaa gccttcgcca gtcacaagcg ctacccctgt ttcttctaaa
    55501 agatcttcta cccattgtgt gacattttca tatttacaca tgcgtagcgt ttcttttaca
    55561 ttaggaaaaa gatagaaagc tccttgcggc ttttcaagag aaacacctgg cagttctgcc
    55621 acaagcggat ataacgtatt caggcgttct tcaaaggcgt tccgcatttc ttctactgta
    55681 tcttgctcac ctttcaatgc ttcaacagct gcatattgac tgactgctgt cggattactg
    55741 gttgactggg aagcaacagc aatcattcca ttgatgatct cagaattccc caccgcataa
    55801 ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc cattgatgat gatggtctga
    55861 tttttgattg cttctgaaat cgttgcaatt ggagtaaact cattgccatt ataaactaaa
    55921 cgaccataaa tatcatcagc aactatcaaa atatcatttt ttactgccca ttcaccgatt
    55981 gcctgaagtt cattcttgct ataaatcatt ccagtcggat tcgatggaga attgatgatt
    56041 acggcttttg ttttttctga acgtgcttgt tctagctgtt caacagtaac tttccagcta
    56101 ttggattctt cccctttcac aaagacagga agtccttctg ccagttttac ttgctcaccg
    56161 taactgaccc agtaaggcac aggaatgatg acttcatctt gcgggtcaag gatcgtttgg
    56221 aaaagggtat acaaagcaaa tttcgctccg tctgttacga ctgtttgtga aggttcatag
    56281 acaagtccat agtttttctt cagataatca acgattgcct gtcgaagctc tgggattcct
    56341 gctgttggtg tataatagct agctttccca ttgcggattg cttcgattgc tgcttcttgg
    56401 atgttttctg gtgtcgcaaa atccggttct cccacagtca aactcagcac atctctgcct
    56461 tttgctttta atgccttggc ctttgccgca gctgccaaag tgacagacgg ttctaatcgt
    56521 tttgttcttt ttgataactc catcccattc attcctttac cttttaaatt cccttgattg
    56581 tcttgacttc atctccagat tcaaagccaa tcaggtaata attcagtttc ccatttgttt
    56641 cttttcctac tacttcccac acaggctgat catcatacat ccctaaagca gctttttcga
    56701 tttggatttc aggatgggcc gcagcaattg tctgttttgc ttcgttttca gtcagtccct
    56761 ttgtttgatc caatactttt actttttcac cagatttagg aatgataacg gcgatttctt
    56821 ttcctttatc atttgtacct gtcagtgaaa agtatgtctt ctttcgagta aaccaataaa
    56881 aatgatcggc ttcctcaaga tttgcatatt tctttgcgat ttctactgct tctttcttcg
    56941 cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga gctgatcacg atgaccgcta
    57001 ataaaaatac gatcaagcca agtaacacgc gattaatcgt tttttccttt gtctgttttt
    57061 tcttcaaggg tcgtcgctcc tttatcttca ttccttttca ttatagcaga ttttaattat
    57121 gaaaaaagac agctcctcta acttccttct tcatttttct ttaagaattc atggatgtcc
    57181 tgatagattt cctccatagc tagctctttg atcggtactt ttttcggtaa tgcatggatg
    57241 atccgtgcac catattttgc agtgactagt cttcgatcca aaataagcaa tacgccacga
    57301 tctgtctccg tccttatcaa gcgacctaat gcttggcgta acttcaaagc tgctttaggt
    57361 acggattcct ggtaaaaagg attgatcccc tctgattcta aataagcgtt cctagctttg
    57421 accatcgggc gaagcggatt atcgaagggt aaacgtgtga ctacaacgat ctgcaaagca
    57481 tccccaggca aatctacacc ttcccaaaag ctgtcagcgc caaacaatac gcttttttct
    57541 gacaaaatga agcgtttcaa aagcttctcc cggcttccgc caaacccttg agctagtatt
    57601 tctcgtcctt tttctagcat agacagatgc attttctgat agaccttctg caaaacatca
    57661 tgagaagtga ataacacaag tattggccga tcttcttgtt cgatcagacc tgtaagtgct
    57721 gtctctaaat aatcgatata tgcttgattg ctgacttgat ggacggcagg tgcctctttt
    57781 ggcacataga tccttgcctg tttctcataa tcataaggag aggcaatcac tttaagaggt
    57841 gctgcttcaa tgccaagccg cttagagaga tagtgtcgat tagggccgat cttcagcgtt
    57901 cctccaatat aaagaatacg atcatatcga ggataccaaa cagtcgacgg caacatagcc
    57961 gcttgaaaat catggagatg aaaaatcatc tgctgcgctg tgttcccagg aacgaaccaa
    58021 tggatcaaat gatctcgcca gtcttccagc cattgatcca tcagcaagta ctgcttgtct
    58081 aattgatcaa ataagctata taaacttgct aaatcgatcc gcatctggtt tgtccagctt
    58141 tcttggataa tctcaaaagt cagacgcatt tgtgactgta gctgctggat ctcctgataa
    58201 aaaagctgca ggtgatcaat ggctgtccca gattccagtg gcagctgatc caacatctct
    58261 tttgtcatca tgacttcttc tggatactgt ccgttaggtt gcttgccgct cactaaactc
    58321 attaatcctt ctgccataca aatctgtgct tcgcatagcg cagaaagttc ttctcgataa
    58381 atcgataacc agcgtaaggc ttcatgttct ttcggtaaat agctttgtat tcggtcaaac
    58441 agctgatttg gttcttctaa ttgctggatt tgtttatgga aatagttaat acttaatcgt
    58501 ttgtcattta ctttttcagc gatttccggc aaatgatgta cttcatcgat caacaaaaat
    58561 ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt gtattaaaaa cgcatgattg
    58621 acaatcaaaa aattactttg cctgattttc ttttgcaaga aacgaacaaa gtcttgttcg
    58681 taaaatgact gatttcttgc taaaaatcca atacctctgt gcgcgatatc tgcaaagaat
    58741 gaatggttca aatttgttaa atgtaattca tccaaatcac cggtcttcgt ttgcgtcagc
    58801 caaactaaga ccgccatttg atacaaagca tattgttttt gttctggcgg ttccacaagt
    58861 gtgcctttaa aacgctgtaa atcaatatag tggcgatagc ttttgataat tacaggttgt
    58921 atgggctgat ctagcaattg attcagcaat ggaatgtctt tttctatgat ctgctgctgc
    58981 aaaactagcg aaacggtact gatgacagcg ggtttttcag gtgtagccaa ataactcaat
    59041 ggcagcagat aaccgattgt tttacccatt cctgtagctg cttcgatcat caaatctttt
    59101 gattcatctt ttgtgaaatg gtcgtagacc gcattcatca gtctgttctg ttcttttcga
    59161 tacatcagtg ttttaccaaa cattttttct tttgcttgtt tttttcttgg atatgtccgc
    59221 tcgccataat ggacggatgt gaatagttcc acttcttttt tctgcaaagc tagcccatcg
    59281 acgatttcta atgagggatc aagtggctct ggtttttctt tcatttcttc aatcacatga
    59341 taaatgaaac gactcgtatc catacctgtg tgtctactta atcgcgcaat ctgctctaac
    59401 gtgatgattg gcaattttct catccgttct tcgatcagca aaagaagttg acccgtcact
    59461 tcggcatcac tatctgcctg atgaggattc tcatgaataa atccaaggct ttctgacaaa
    59521 tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt gtgcaagttc tactgtatcg
    59581 atgcctggta tcttcaaact aggaagtccg catcgcatga gttcatggtt caaaaaatta
    59641 taatcaaaat aaatattatg cgcgacaaat atggtgtctg ccaaaagatt gtaaatcgtt
    59701 tgggcgacat cttcgaaata aggcgctttt tggacgcgtt gattcgtgat ccctgttaaa
    59761 tgctggattt gttttgaaat cttccgccct ggatgaatat cgatcgaaaa acgtgaagtg
    59821 atccgacctg attcaatcat gacgcagcca aattggatga tccgatcttc ttttggattc
    59881 gtcccagtcg tttcgatatc aacgactgca taagttcttg gtttcaaaat atcttcctct
    59941 tctcctgcct ctaatgttct tttgaaatta tactacaaag tgcagaaaaa tactctcatc
    60001 gctttttaaa gaaaatagac aaaaacgccc agacacgcaa atgtctgggc gtttattttt
    60061 tagtataggt taattaagct ttagtaacgt ttgttgcttg aggtccgcgt tggccttctt
    60121 caacatcgaa agttactgct tggccttctt ctaaagtttt gaatccgtcg ccttggatag
    60181 ctgagaaatg aacgaataca tcgtttccgt tttctgcagt gatgaatcca aatccttttt
    60241 ctgagttaaa ccattttact gtacctgttt ccataataaa aatcctcctt gtgtatcaac
    60301 acatattttg caattacttg aatggtgtat atctggaaga tgtttatttg aaacaagcta
    60361 ccacagatta ccgaacaaaa actactccta tagtatatca taccgtttac aaaaaagcta
    60421 gagtttttcg acttttttta ttaaaaaaag ttttctcagc gattacataa agtgaaaaac
    60481 cgaacttttt ttaaaataaa cgacatattg ttcagttggt gtttttttgc tctgatgttc
    60541 ttctttaaac taaaaaaatt attctttctg actacttatc ggttctgttt caacggtcga
    60601 ggctggggaa gattctttag taggtctgat gttctcatgg tatttctgat agcctatgac
    60661 agttaccaga agaacaaggc aaaccaatac gatcaacgag attagttttt cttttttgct
    60721 catatctata cctcataaat aacaggatga tgtccatcat atttctgagt atgagtaaaa
    60781 gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac tttcataatc acaggtcgga
    60841 cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc aggaatcgca
    60901 gaatcaagat ctgttgtctg tacataaaca tttttgtaca gacgtgtgcc tgtaatagga
    60961 gaccgctgtg tttcttcagc aaaaactggc tgacttgtta aacttaggga tagaaacagc
    61021 aaacaccata acagtctttt tttcataaag acctccttat cgataagttt ttcccaatta
    61081 aaatcatatc tcaagaacgc ccgttattca cattttgttc ataaatgatg ttttttgtat
    61141 cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc tttttt
//
LOCUS       DAFGFZ010000003        56116 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000003 DAFGFZ010000000
VERSION     DAFGFZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.003"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(1..1569)
                     /locus_tag="LR726_000142"
     CDS             complement(1..1569)
                     /locus_tag="LR726_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL6215259.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1573..1983)
                     /locus_tag="LR726_000143"
     CDS             complement(1573..1983)
                     /locus_tag="LR726_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL6215260.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1980..2903)
                     /locus_tag="LR726_000144"
     CDS             complement(1980..2903)
                     /locus_tag="LR726_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL6215261.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2900..3778)
                     /locus_tag="LR726_000145"
     CDS             complement(2900..3778)
                     /locus_tag="LR726_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL6215262.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3765..5102)
                     /locus_tag="LR726_000146"
     CDS             complement(3765..5102)
                     /locus_tag="LR726_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL6215263.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5105..6208)
                     /locus_tag="LR726_000147"
     CDS             complement(5105..6208)
                     /locus_tag="LR726_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL6215264.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6282..7790)
                     /locus_tag="LR726_000148"
     CDS             complement(6282..7790)
                     /locus_tag="LR726_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL6215265.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7756..8448)
                     /locus_tag="LR726_000149"
     CDS             complement(7756..8448)
                     /locus_tag="LR726_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL6215266.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8470..9444)
                     /locus_tag="LR726_000150"
     CDS             complement(8470..9444)
                     /locus_tag="LR726_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6215267.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9744..10616
                     /locus_tag="LR726_000151"
     CDS             9744..10616
                     /locus_tag="LR726_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6215268.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11074..13272)
                     /locus_tag="LR726_000152"
     CDS             complement(11074..13272)
                     /locus_tag="LR726_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215269.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13504..14496
                     /locus_tag="LR726_000153"
     CDS             13504..14496
                     /locus_tag="LR726_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6215270.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14556..15977
                     /locus_tag="LR726_000154"
     CDS             14556..15977
                     /locus_tag="LR726_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL6215271.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15970..17469
                     /locus_tag="LR726_000155"
     CDS             15970..17469
                     /locus_tag="LR726_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL6215272.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17508..18902
                     /gene="uxaC"
                     /locus_tag="LR726_000156"
     CDS             17508..18902
                     /gene="uxaC"
                     /locus_tag="LR726_000156"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL6215273.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19061..19744
                     /locus_tag="LR726_000157"
     CDS             19061..19744
                     /locus_tag="LR726_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL6215274.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19746..20582
                     /locus_tag="LR726_000158"
     CDS             19746..20582
                     /locus_tag="LR726_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL6215275.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20593..22347
                     /locus_tag="LR726_000159"
     CDS             20593..22347
                     /locus_tag="LR726_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6215276.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22623..22964)
                     /locus_tag="LR726_000160"
     CDS             complement(22623..22964)
                     /locus_tag="LR726_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL6215277.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23032..25203)
                     /locus_tag="LR726_000161"
     CDS             complement(23032..25203)
                     /locus_tag="LR726_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL6215278.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25372..26271
                     /locus_tag="LR726_000162"
     CDS             25372..26271
                     /locus_tag="LR726_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL6215279.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26342..27199)
                     /locus_tag="LR726_000163"
     CDS             complement(26342..27199)
                     /locus_tag="LR726_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL6215280.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27184..29886)
                     /locus_tag="LR726_000164"
     CDS             complement(27184..29886)
                     /locus_tag="LR726_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL6215281.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29899..31188)
                     /locus_tag="LR726_000165"
     CDS             complement(29899..31188)
                     /locus_tag="LR726_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL6215282.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31338..32384
                     /locus_tag="LR726_000166"
     CDS             31338..32384
                     /locus_tag="LR726_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6215283.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32386..34539
                     /locus_tag="LR726_000167"
     CDS             32386..34539
                     /locus_tag="LR726_000167"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL6215284.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35012..36463)
                     /locus_tag="LR726_000168"
     CDS             complement(35012..36463)
                     /locus_tag="LR726_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6215285.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36460..38184)
                     /locus_tag="LR726_000169"
     CDS             complement(36460..38184)
                     /locus_tag="LR726_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL6215286.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38177..38791)
                     /locus_tag="LR726_000170"
     CDS             complement(38177..38791)
                     /locus_tag="LR726_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL6215287.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39136..40593)
                     /locus_tag="LR726_000171"
     CDS             complement(39136..40593)
                     /locus_tag="LR726_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6215288.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40634..41554)
                     /locus_tag="LR726_000172"
     CDS             complement(40634..41554)
                     /locus_tag="LR726_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6215289.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41566..42513)
                     /locus_tag="LR726_000173"
     CDS             complement(41566..42513)
                     /locus_tag="LR726_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6215290.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(42992..43711)
                     /locus_tag="LR726_000174"
     CDS             complement(42992..43711)
                     /locus_tag="LR726_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL6215291.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(43760..45406)
                     /locus_tag="LR726_000175"
     CDS             complement(43760..45406)
                     /locus_tag="LR726_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL6215292.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            45585..46175
                     /locus_tag="LR726_000176"
     CDS             45585..46175
                     /locus_tag="LR726_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL6215293.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            46267..47772
                     /locus_tag="LR726_000177"
     CDS             46267..47772
                     /locus_tag="LR726_000177"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL6215294.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(47856..49208)
                     /gene="celB"
                     /locus_tag="LR726_000178"
     CDS             complement(47856..49208)
                     /gene="celB"
                     /locus_tag="LR726_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL6215295.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(49208..49726)
                     /locus_tag="LR726_000179"
     CDS             complement(49208..49726)
                     /locus_tag="LR726_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215296.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(49742..50068)
                     /locus_tag="LR726_000180"
     CDS             complement(49742..50068)
                     /locus_tag="LR726_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL6215297.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(50081..52060)
                     /locus_tag="LR726_000181"
     CDS             complement(50081..52060)
                     /locus_tag="LR726_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL6215298.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(52081..52395)
                     /locus_tag="LR726_000182"
     CDS             complement(52081..52395)
                     /locus_tag="LR726_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL6215299.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            52563..52856
                     /locus_tag="LR726_000183"
     CDS             52563..52856
                     /locus_tag="LR726_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL6215300.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(52934..54046)
                     /locus_tag="LR726_000184"
     CDS             complement(52934..54046)
                     /locus_tag="LR726_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL6215301.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            54199..54423
                     /locus_tag="LR726_000185"
     CDS             54199..54423
                     /locus_tag="LR726_000185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215302.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            54433..54612
                     /locus_tag="LR726_000186"
     CDS             54433..54612
                     /locus_tag="LR726_000186"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215303.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            54806..54976
                     /locus_tag="LR726_000187"
     CDS             54806..54976
                     /locus_tag="LR726_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6215304.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(55400..>56116)
                     /locus_tag="LR726_000188"
     CDS             complement(55400..>56116)
                     /locus_tag="LR726_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL6215305.1"
                     /translation="PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKL
                     AIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKI
                     DDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYEL
                     DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFIL
                     SILGVLLVFISIKFRKKRNI"
BASE COUNT        16730 a        11476 c         9889 g        18021 t
ORIGIN      
        1 ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac ttcctctttt
       61 tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc ctgagagtat
      121 acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt ctttcaaatg
      181 aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg
      241 aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac gtaggcattt
      301 tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat ttaagtacag
      361 taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg tctccgcatc
      421 aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag attgaagaga
      481 ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc aatcagcatt
      541 tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg tatccataaa
      601 ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt cttccatcgg
      661 agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca cttcttgaat
      721 agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa gatatgtcat
      781 gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt gcaatgtaga
      841 agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt aaggaccagt
      901 aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc cttctgccgt
      961 catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt tcgctaattc
     1021 tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg catcatattc
     1081 ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc atagttcatt
     1141 gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa agccgttact
     1201 atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga tcaaataatt
     1261 gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa gataaaacaa
     1321 tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat ttccttgcaa
     1381 agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat ttttcgactc
     1441 atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat accgctcttt
     1501 cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt tcactttgat
     1561 agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga atgccccagc
     1621 tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca acaatatgaa
     1681 tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag cagtcaaaaa
     1741 caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca gaccagtcat
     1801 gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta cagcaagcga
     1861 ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg ctaacccgcc
     1921 aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc attttctttt
     1981 catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat cgatgtttca
     2041 taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc atcccaatta
     2101 tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa gacatttgca
     2161 tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc agtcaaggta
     2221 cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc tgtgatgtat
     2281 ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc atctgcccca
     2341 ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg acgacaatac
     2401 tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac
     2461 ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa agatacgcga
     2521 tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc tgctgtattt
     2581 tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt agcacttcgg
     2641 aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt accggagata
     2701 actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat atattcatca
     2761 tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc tgccaaataa
     2821 tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt
     2881 atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa atatttctca
     2941 tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt tctggactac
     3001 ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct tcgtccaagc
     3061 acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt ttttcttgga
     3121 ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac ccatgatgat
     3181 ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga tcaatacaaa
     3241 ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc atagaaagac
     3301 caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc
     3361 tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa aattgtttga
     3421 acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa
     3481 cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag taaggagcac
     3541 catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga
     3601 ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact gtttccaatt
     3661 catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct cggtataatc
     3721 gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc aactgcataa
     3781 gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga ttggaatttc
     3841 tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg attttcaatc
     3901 gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg tctgctcatt
     3961 tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc catcgagaca
     4021 tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata aataaaacct
     4081 gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct gaatgctggt
     4141 gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt ccctcgcgga
     4201 ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat gactatattg
     4261 gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa tcgtattcct
     4321 cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact accactcatt
     4381 tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt gatcgtgata
     4441 ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc aatacagtca
     4501 tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc tggatcaatt
     4561 ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa cgttgcatta
     4621 ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat atctttcaca
     4681 gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc gagattatct
     4741 ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt accgaaacca
     4801 gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac ttttcgatgg
     4861 actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga gaatttcaga
     4921 accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc tcctgttaga
     4981 aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc atcaatggct
     5041 tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa aatatcatac
     5101 atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat
     5161 cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc tgatctgctc
     5221 cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct tttgtttcca
     5281 gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc cattcttctt
     5341 tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata ctactgccgg
     5401 aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac catgtcccgc
     5461 tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt tctttcagca
     5521 aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc atcaaatacc
     5581 agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca gcccatggtt
     5641 ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg aatagatgtt
     5701 tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca ctaaaattct
     5761 ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa atttgataag
     5821 gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt gccaactgct
     5881 gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg tacaataaaa
     5941 agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg tagccacgga
     6001 tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca aaataaattt
     6061 tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca tagtcataag
     6121 accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc atgatacttt
     6181 ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt aaaataggat
     6241 ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc cgctgctttg
     6301 tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca gtcttctaca
     6361 gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt agcatcaatg
     6421 acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa
     6481 ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa ttctttttca
     6541 gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga taacggttgc
     6601 acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc attcaattca
     6661 taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc taaaagatca
     6721 tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga tttgggaaat
     6781 gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc gccttcacca
     6841 tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg acccatcgtc
     6901 ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg tgcaaattgt
     6961 tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc attctcaaat
     7021 agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc tggagtgaat
     7081 ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt gaaaagttta
     7141 aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt gtcgtccaca
     7201 ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag ctcatccatg
     7261 gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac taagccagca
     7321 cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact cgcagcttcg
     7381 attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc tgatacatcc
     7441 cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat cgcaatttga
     7501 agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct ttcttctttt
     7561 gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc
     7621 acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt gattttagct
     7681 tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc ggcaagtgct
     7741 agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat aaattttctc
     7801 cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg agttcttcca
     7861 ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga agagactgcc
     7921 accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa tccctaccat
     7981 acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa atgccatttt
     8041 cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa tatagagtgc
     8101 tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc aattgtgtat
     8161 gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc tcatcagcaa
     8221 ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc
     8281 ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag gaccgaaagt
     8341 aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat aaaccaactg
     8401 taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat ctttccctct
     8461 ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat tagctaaaat
     8521 taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga aacttaactg
     8581 tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt
     8641 caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct gaccgatttt
     8701 tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat gccatgtttt
     8761 gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat cgactgcagt
     8821 tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca cttgtccgat
     8881 atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa atcccattgt
     8941 tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag taatcgaaac
     9001 aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa
     9061 tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg gaaaaaacac
     9121 gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga atgtcggttc
     9181 tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta ttcctaaaaa
     9241 tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc taaaaatcaa
     9301 gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt tgattcctgc
     9361 aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca gttgaaatgg
     9421 ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt ttgctgctta
     9481 tgacttcatg atagataact atctgaatta cgtatataat catttttacc gtaagtaaaa
     9541 acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa
     9601 taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat aggaaatgat
     9661 tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa catttaattt
     9721 attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa agaagtttta
     9781 cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt ccttttttaa
     9841 atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag tttatccttt
     9901 ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg accattttta
     9961 gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa
    10021 cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact
    10081 ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc ttagttgttc
    10141 gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct gttaatgcaa
    10201 tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg gaagaatcag
    10261 caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg ccattagcgt
    10321 taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac acttatatga
    10381 ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt ttacgccaaa
    10441 tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg attccaccag
    10501 cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg attgtttatc
    10561 cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat
    10621 gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa taagaaggca
    10681 tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg aagaatctac
    10741 tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag cgatgataaa
    10801 aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa
    10861 tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc ttgaacgcca
    10921 tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg cgtgcttagc
    10981 cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc ttttattgct
    11041 tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc gatattctcc
    11101 cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat ttcgatattg
    11161 caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt tttttgctac
    11221 ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct ttttaaatac
    11281 attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta aagacagatc
    11341 acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt taaattcatg
    11401 atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta aaacaagttt
    11461 ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt tcatgttatc
    11521 aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat tgatcaagcg
    11581 gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg catcaatcaa
    11641 ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa acaatcgatt
    11701 ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa cgatttcctg
    11761 ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt cttttgcccg
    11821 atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc ctatactgat
    11881 agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact ccatgacttt
    11941 tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca tttcttcatt
    12001 caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca tgttgttgat
    12061 cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat cgatttgaac
    12121 caaagctgta taatagcagt cattttgaga actataacca aattgctgca accgctgatc
    12181 tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg tttccagctg
    12241 tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga tccgactgac
    12301 tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa gattcagacg
    12361 ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg aaagaagacc
    12421 aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat tgcggatcat
    12481 tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat aatctgattt
    12541 cagtactata tacgttttat ctttttctct tatcactttc agctgattgt aatctagatc
    12601 cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag taaaaaggtt
    12661 gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa cttgttctat
    12721 tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa acattggcaa
    12781 agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat ttttgttatt
    12841 tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca acgatccatt
    12901 gattactccc catttaccag ccaaactgac taaatcataa ttattatcat ccataacaat
    12961 cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat ctgtatatgt
    13021 cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata
    13081 ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca aattcttttt
    13141 gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga ataaactaaa
    13201 tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat aaaatactgt
    13261 acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat ttttctgtaa
    13321 aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca taattaaaaa
    13381 taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc tattgactta
    13441 tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat
    13501 ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac gacagtttct
    13561 cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat acgacaacta
    13621 gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa
    13681 tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa tttcctagca
    13741 gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc tgttgaaagt
    13801 atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact gatcgttatg
    13861 agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga atttcctttc
    13921 gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc cgatgatgcg
    13981 gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa gatcgcttat
    14041 attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt
    14101 ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga ttacagctta
    14161 gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc tactctggtt
    14221 ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt agctggcctc
    14281 cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc
    14341 aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag
    14401 cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga gctctccacc
    14461 ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt tatataaaaa
    14521 tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca aaagaattag
    14581 tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg
    14641 gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa ggtaatgttg
    14701 caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa gataatttat
    14761 atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc gagattatca
    14821 ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta tctctagcag
    14881 aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc caattcgtgg
    14941 agactgataa tttaacagat actcccccac agagcctccc gggcaaactg acagcctttt
    15001 tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc gaactgattg
    15061 acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa tgggatttag
    15121 aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc
    15181 gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg cataactatg
    15241 aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag
    15301 aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc acagatgata
    15361 tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca gcgatggtcc
    15421 ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat cctttattct
    15481 ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct ttacctaaag
    15541 atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct tttgcccatc
    15601 acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag
    15661 tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact gtttcactag
    15721 ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat gaaaagacga
    15781 taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga ttattgcaaa
    15841 acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat acagttcaag
    15901 aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa atgaaaggaa
    15961 cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg agacagtgtg
    16021 gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca gcaaacgatc
    16081 accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt gaaagctgga
    16141 gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt aactgccggt
    16201 caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga ttatcgatat
    16261 gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca aggttacttg
    16321 cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac cgttgggtgt
    16381 gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc agatctggga
    16441 cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct tggaaaagat
    16501 catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc cggaggagtt
    16561 ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa aattttaggt
    16621 gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga tgagatcgaa
    16681 caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca gcgcatatca
    16741 gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg tctatcggga
    16801 ataactgcta atcctttact tggtgcattt tctgactact tgattgctca aggaggcagc
    16861 acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat ggcacgagcg
    16921 gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa acagtatttc
    16981 ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc aggtgggatc
    17041 actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt
    17101 gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt gcaagctccc
    17161 gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt ggtcttgttt
    17221 accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt atctacgaat
    17281 acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt cttattagaa
    17341 caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga
    17401 gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa aaacggagta
    17461 acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg tttttatctg
    17521 atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac gcaaaaaaca
    17581 tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa aacaacaatt
    17641 tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc ttgatgcgag
    17701 cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa aaatttttag
    17761 cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg tcacatcttg
    17821 aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca gagaaaattt
    17881 ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa
    17941 aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg cactatcatc
    18001 agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt ccagataaag
    18061 cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa gaagcagctg
    18121 accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg atagaatact
    18181 tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc tataaagaag
    18241 cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa ctattatctt
    18301 cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac ttctatcatt
    18361 ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat acacaaatgt
    18421 tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct ttgactattc
    18481 ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag acgatccttt
    18541 attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt ttccaaaaag
    18601 aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact cgagctggta
    18661 tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc
    18721 tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt cgtgttcttt
    18781 gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa caacttttag
    18841 gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc ttttttgcct
    18901 aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac ggaatccgtt
    18961 tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct cttgctgttc
    19021 tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta tccatattgc
    19081 cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag gttggggaga
    19141 aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa agaatggacg
    19201 cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac agtttcagcc
    19261 gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag aagaaagagg
    19321 tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa
    19381 agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact tagaaaacgg
    19441 aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa
    19501 acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc agaccttctc
    19561 aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta aaaattaccc
    19621 agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag ttgcccgaat
    19681 aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac aaaaaggagt
    19741 ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg
    19801 aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt gcaccacaaa
    19861 aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct gcctattttg
    19921 gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat atcaacctat
    19981 ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag caaattgctc
    20041 tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc tctgtcggtt
    20101 acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct atcattgaat
    20161 tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc
    20221 caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca ccaaatttaa
    20281 aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat caggaaaaag
    20341 tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat ctcaaagact
    20401 atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt ttagatttcc
    20461 gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact cttgaagcta
    20521 ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac caagattgtt
    20581 aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca tgcaaatgct
    20641 tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat gtcaggactc
    20701 ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc caatgtaatc
    20761 aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa cgcttttttg
    20821 atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa ataccatagc
    20881 acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa aatccaagaa
    20941 ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa gatcatccgt
    21001 gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt aaatatcgct
    21061 gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt tttcatgttt
    21121 ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa aaggatcaga
    21181 cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg
    21241 atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac aactcgctta
    21301 aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct gcaatctgtt
    21361 ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt gattttcact
    21421 gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat
    21481 ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac gatttcaaaa
    21541 ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt agaaacttat
    21601 cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga tgtttctttt
    21661 ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt gaaagctgga
    21721 gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct taacttattg
    21781 attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc tttggatgaa
    21841 ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac tttgttgttc
    21901 tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa agaacgttta
    21961 gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc tgatggtctg
    22021 gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca acgtatctcc
    22081 attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc aacctcagct
    22141 ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac agaaacaccc
    22201 actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa aatcgttgtg
    22261 atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc tcaaaaaggc
    22321 tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag acttttgtcg
    22381 tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    22441 agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag agttaaacgg
    22501 taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca ataagaagca
    22561 attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt cataaaatag
    22621 ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa gtcattgatg
    22681 attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc tgattggatt
    22741 ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg tccactcatt
    22801 tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct
    22861 tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg taattcacgg
    22921 atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg ccctcttttc
    22981 ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg gctactctcc
    23041 ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc cttctttcac
    23101 catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat tcaagttatc
    23161 ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat ctgcagctac
    23221 cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg aagtatacgg
    23281 aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac ttgtcccttc
    23341 caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca ctacgtctgg
    23401 cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag tttctgtggt
    23461 tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg ttccatttga
    23521 aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata tgacctgttt
    23581 tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga ttttcgtgat
    23641 aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat
    23701 caatggagat actccttttt ccagtccacc aagggctaag ccataatact tatcagattt
    23761 tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac cgatctcatg
    23821 caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca tcggtacttc
    23881 tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa
    23941 gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta atggtttgat
    24001 cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa agacatgatc
    24061 tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag ctactgaagc
    24121 agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt tttgataagt
    24181 agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat agcctttatt
    24241 catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca ctgcatcaaa
    24301 ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa gatacgcgct
    24361 gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt tttcttccat
    24421 tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga tcggattata
    24481 aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg taacattcgt
    24541 tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc cgttgccaaa
    24601 ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct tttcgatctc
    24661 gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc
    24721 atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc cgccagacaa
    24781 gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat gctcgtaaaa
    24841 attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg cgtttaattc
    24901 tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct tatcataaat
    24961 gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat
    25021 aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt
    25081 cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga gatatggtct
    25141 gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc
    25201 catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata gtgaaagagt
    25261 gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc aagtagagta
    25321 acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa aatggtacaa
    25381 ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac tttacctatg
    25441 aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt tcctagaaag
    25501 gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc tgctttgttc
    25561 catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac agattataat
    25621 tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga agatgagcat
    25681 ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc gacagaaaac
    25741 agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg accttacgtt
    25801 gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc
    25861 ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata tcaagccatc
    25921 gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg agatcgccat
    25981 gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac acatgtcact
    26041 gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac cggacgtaca
    26101 caccagatca gagttcattt agctcataag ggacatccga tcgttggaga ccctctctac
    26161 caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat gatccatcca
    26221 tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata gaaataaaaa
    26281 agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt aatttgtctg
    26341 cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca ctttcattcg
    26401 gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa ccacccatca
    26461 attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt accccaccgc
    26521 ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct tgagcaatat
    26581 aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct ttgatccctg
    26641 tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta tgataaggac
    26701 aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc atttcaggat
    26761 gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt cctgttccca
    26821 cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat tccccatatg
    26881 cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc ttgattgtgc
    26941 caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat ccgtaattat
    27001 cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc ttcaattcat
    27061 aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg gtagggatac
    27121 ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt gttccgcctg
    27181 cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa tctcataagg
    27241 ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca atatgacagt
    27301 ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat tgaaacctct
    27361 tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa acgtttcact
    27421 ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt tgatttgttg
    27481 agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg
    27541 tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg cttctggtgt
    27601 tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca ctgcgatttg
    27661 tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta caccgtactc
    27721 ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt tttcccaact
    27781 tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc
    27841 cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct gattgtcgag
    27901 agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg tttttatttg
    27961 aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca caaccatctg
    28021 ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct gtagaatact
    28081 tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt ctttgttact
    28141 ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc ctatatcgcc
    28201 gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat cttccattgt
    28261 aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga ttcgtccact
    28321 ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt
    28381 ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca ttcttgccat
    28441 atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag tttcttcaga
    28501 taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac gtttaggcaa
    28561 attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt ttctttctaa
    28621 ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta caatgacaaa
    28681 tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct gttctaagga
    28741 atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta tcatttcccg
    28801 atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt gcattaatgt
    28861 cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat aagccatcgt
    28921 cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc attgttttaa
    28981 gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt cttgacttgt
    29041 caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg cttccagata
    29101 gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg cgagatggat
    29161 tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca tcaataagtg
    29221 gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg caagagctgc
    29281 ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata gcagaccaaa
    29341 gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag aatagttttc
    29401 tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa acgctgctgc
    29461 tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa atgtatcggg
    29521 aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc caatcagcat
    29581 atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat aaaatggtcc
    29641 aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt ctggccttac
    29701 ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg
    29761 atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca tgtgatgctg
    29821 ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat atactttctt
    29881 tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca tgactaattc
    29941 gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag gattgttcac
    30001 atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat
    30061 ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg caatcggcca
    30121 aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc cttcatagaa
    30181 atatggattt tctttactca acaatgttct tcttgtttgc agataatctt cctcttgttc
    30241 agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt catccatgac
    30301 tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt tttgattaac
    30361 cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct gtgtttgctg
    30421 tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac ctaacacgac
    30481 caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat cgctaggacg
    30541 gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag
    30601 cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt catgatcttg
    30661 ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt gattgaattg
    30721 atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt agcataaaga
    30781 atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat ctgtttggtg
    30841 gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat tgatataaaa
    30901 aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt cttttgcaat
    30961 cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc
    31021 cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca gagtattggc
    31081 aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt tttgtgtgat
    31141 atcttccata aatttctgca ccgattttgg tatatcttta taagccacta ataaaacctt
    31201 ctttctattt gatatatctt taaagattat acttcataca gccaattatg tctacgcttt
    31261 caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa tatgatatat
    31321 cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat gatttgcaaa
    31381 cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa aaagaacttt
    31441 cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa ttagagcaat
    31501 taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct tctccggaac
    31561 agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca tatgtcgctg
    31621 atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat aaacagattg
    31681 atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag ctaatcaatg
    31741 aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac ttactttttg
    31801 agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat gagttacctt
    31861 ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct tatttgatcg
    31921 aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca caatccgttc
    31981 gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact ttccacactt
    32041 cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact aagtatgaag
    32101 taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat ctcttgagac
    32161 agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat atccaagcag
    32221 cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc
    32281 ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc gatagtaaat
    32341 ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa
    32401 atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa ctgccagcct
    32461 tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga tcaaaacggc
    32521 agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac ccatcaacca
    32581 agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg aaaactacag
    32641 gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat attttgcccg
    32701 actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat acctagcatg
    32761 tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact tttatccttt
    32821 ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa aatcacacat
    32881 tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt tgaatatcct
    32941 ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc gttgtatttt
    33001 gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc ggaacaagca
    33061 gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa
    33121 gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca acaacaattg
    33181 cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt ttatgaaatt
    33241 gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa acctggtgtt
    33301 ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct ttattctttg
    33361 attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta tagagaaagt
    33421 ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg gacattgatc
    33481 gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat gcctgaattt
    33541 ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta tggacgccaa
    33601 ggaacagaag attacttgac atatggctat gttcctaatc actaccacga atcagtcaat
    33661 cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa ggtactagaa
    33721 gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa tatctttgac
    33781 tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga accattttca
    33841 gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt
    33901 tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata taaagaaaaa
    33961 ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg ttttgagatc
    34021 cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc aaaccagcca
    34081 agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc acagccactg
    34141 cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc tggagatgag
    34201 gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta tcctgtcacg
    34261 cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt tttgcttttg
    34321 tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca acaatttatc
    34381 cataaaatca aacggatgga caaagaatat acgaaattat attttaccca tcaagatttg
    34441 ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa
    34501 gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt gaaaaagctg
    34561 tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt ttcgtttggt
    34621 tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt tgtgaaatca
    34681 gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgccatt
    34741 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag gtcgtgaaaa
    34801 agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca
    34861 taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag aggtcatgaa
    34921 aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag acaatttttt
    34981 ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt tctcggtact
    35041 ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg gtgttgttgt
    35101 agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc agttgtttcg
    35161 ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt tcttttttga
    35221 acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct ttgagtgtaa
    35281 gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg tcactgtact
    35341 ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt tcttgcaccg
    35401 tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc agatcaatca
    35461 attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct tccggcatca
    35521 tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg acttccaatt
    35581 gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct tttgtttctg
    35641 cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc aactgtagat
    35701 tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc tcttccccga
    35761 tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag aagccatcaa
    35821 cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt cgttgatagt
    35881 aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga gttcttgcca
    35941 gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt tcttcttcac
    36001 ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga tagatttctt
    36061 gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg aggctttcaa
    36121 ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat tgcatacctc
    36181 ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa ttatgacgtt
    36241 ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc acatccgtta
    36301 ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca ttctctgctg
    36361 ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac ccattcaaaa
    36421 tcatgtattc atcatctacc aataacactt gatacacgct catccctcct tgaatgtaat
    36481 agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac tgctgccgaa
    36541 gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa gttccacttt
    36601 aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc cttttccatt
    36661 atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac ttagcgcatt
    36721 atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg gctggatcac
    36781 aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat aggctactcg
    36841 attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca gttcttcttt
    36901 taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa acgcatacac
    36961 aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat attctaatgt
    37021 attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg cgtcttgctg
    37081 tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca acatttcgtt
    37141 gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga tccgcgtctc
    37201 taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt gctgtgaata
    37261 tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa tgataccaat
    37321 gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat ctagtacagc
    37381 taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt tttccagatc
    37441 gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt cttgtctttc
    37501 aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg tcgttccaga
    37561 aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca tatctagttc
    37621 tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg tgataggcaa
    37681 cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc cacctttgtt
    37741 ttctctagaa gattcataat actccggtag ttgattcaac acgatatata tcgcacttag
    37801 ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta attcatcggt
    37861 actgttttgc tgttcgtata catacatcag atactgatct atgggttttg ttaagtaggt
    37921 cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga tcctactcaa
    37981 ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg tcatgttttc
    38041 tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag cagtcatcgt
    38101 tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg ttccttttga
    38161 aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct tcacgccaat
    38221 gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac aggatcaatc
    38281 ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt tttagaaaag
    38341 acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga acagagatcg
    38401 tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag agcaaggcaa
    38461 aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca gataaatata
    38521 aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga ttgcctctta
    38581 aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta tactcgaatc
    38641 cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca aagatgatcc
    38701 cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc cagatccgga
    38761 taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt aagtttatac
    38821 tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta ttagagaaac
    38881 aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga gggttagctg
    38941 attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt agtaagaaaa
    39001 attttgtgaa tatggctttc cacattttta catatattta gcttaatacc gagaaactgg
    39061 cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc acaacccctt
    39121 tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca tttctttcaa
    39181 taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg tttcatctgg
    39241 atcaacagtc cctgtattca atccatctaa atattgattc atgacattag aaatattact
    39301 taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg aagtttctgc
    39361 atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact cttgtgtata
    39421 tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct gataaccatc
    39481 tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc cctcgatccc
    39541 ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa gttctactga
    39601 tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg cttgagctgt
    39661 agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg catttgtcaa
    39721 aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc aagtatttcc
    39781 ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc cttgtttgta
    39841 ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt ggttgatgat
    39901 tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta aaggataatc
    39961 aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt ttggttcttt
    40021 ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc catcaattga
    40081 aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag caaagacatt
    40141 tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa tatatgcttc
    40201 atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca aatcagcaaa
    40261 ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat aattttcccc
    40321 ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt aattgatatt
    40381 gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca attgatcgaa
    40441 attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc tagcttctgc
    40501 ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca acgctgaaac
    40561 acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt ttatgattta
    40621 tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca aaatgcttct
    40681 ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc gccattctgg
    40741 cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct cctgaatttt
    40801 gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt ggaactagct
    40861 tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc aatgcggcaa
    40921 ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga acaaatattc
    40981 gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag accgaacgtt
    41041 taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc aagatcaatg
    41101 cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg gccaccatcc
    41161 ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg aatggaaagt
    41221 ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc agtgtaccta
    41281 aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc atctggtcga
    41341 acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac ccgtgagtcg
    41401 acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc aaaatacagg
    41461 aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta ttgaaagagc
    41521 ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga ataatgcaga
    41581 atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa agccgacaac
    41641 tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg tcaggccatt
    41701 gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat tcctaggaat
    41761 gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa tcgttaaaat
    41821 cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc atttgctagc
    41881 tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag atgcaaagta
    41941 aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga taaacggcca
    42001 atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga tccactgatt
    42061 gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa tgacccaaga
    42121 tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt tatttctcaa
    42181 ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga taaatgctac
    42241 attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa ataaaaattt
    42301 gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc catctgctga
    42361 aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa agaaaaaaat
    42421 catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc atagattcat
    42481 aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga ttcattcgca
    42541 acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg aaaatgaaac
    42601 aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga aaaaaacggg
    42661 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
    42721 accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga gatcgtgtcc
    42781 ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt cttgtcacag
    42841 tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    42901 cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg cgctacaact
    42961 actctgctta ttcttttagc tatccattca attatttttc tctcacttgt tctagctctt
    43021 ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa aattttccca
    43081 tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt cgcttaactt
    43141 ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc tcttgatcta
    43201 cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata atcagtcctt
    43261 ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct agatattctt
    43321 tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca ttcgttaaaa
    43381 tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct tcccgataat
    43441 cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta gcagaaatga
    43501 ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg atttctccta
    43561 aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc accacttgtc
    43621 cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg ctgccaagtc
    43681 ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa agagcatcaa
    43741 tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc gctattcctg
    43801 gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac actaaagccc
    43861 ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct tcgtttaaaa
    43921 aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag gtaagaataa
    43981 agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact tcatggattc
    44041 tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat aaagggccgt
    44101 atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga aacccaataa
    44161 ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg gaaactagca
    44221 tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta cttcctagag
    44281 aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca ccaaatgtcg
    44341 ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt gccagaccta
    44401 ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac gcgatcgatg
    44461 ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa atcgaaaaaa
    44521 gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc ataaaagtga
    44581 actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac ggaatcgctt
    44641 ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt tcgctgtagt
    44701 gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg acaagaatac
    44761 ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc acatagtcac
    44821 catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata cgtgccacct
    44881 gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc aaataccctc
    44941 gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc atcgttggaa
    45001 tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg tacatgaaca
    45061 aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat aatcgtcgtg
    45121 aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc gcagcaggta
    45181 ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata ttgaataatc
    45241 cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata tagacagcgc
    45301 ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt gccatttttt
    45361 cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt cagattccaa
    45421 ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca atcgatttta
    45481 tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt ggtaaagtaa
    45541 agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca caagaaataa
    45601 aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg aattggttct
    45661 tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta gttgaaacgg
    45721 ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg ctgcttctag
    45781 ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt ctgctttttc
    45841 ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc ttttcactga
    45901 tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc ggggcctcaa
    45961 atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt ccaatgccgc
    46021 aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt tttggattgt
    46081 tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg aaaatactca
    46141 tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca tagcggaaac
    46201 aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa agcgagggat
    46261 caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga aaatctatta
    46321 aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc tgaatttctt
    46381 cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg taaaggaatg
    46441 aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata ttatgtatca
    46501 gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg taaagcaatg
    46561 gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt gatcggattc
    46621 actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt ggatcacaca
    46681 acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg caaaacatat
    46741 ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat gtcagaagca
    46801 gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta caaaacacaa
    46861 cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc tgaccatatc
    46921 agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca attgatcttg
    46981 aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact tagggaaaca
    47041 gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag caattatcaa
    47101 gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga agatcgctta
    47161 gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca agaaaatgca
    47221 gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc acaagacgga
    47281 ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa tggagcgcat
    47341 gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa atttgccaaa
    47401 aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa caaagcaatc
    47461 tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc ctttttgacg
    47521 gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa tgaagcaatt
    47581 actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat tcgacgcgct
    47641 ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt ggatccctat
    47701 caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt gaagcggttg
    47761 attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa aaaagggact
    47821 gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat
    47881 tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt gataaatggg
    47941 aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca
    48001 ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc
    48061 atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga
    48121 gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt
    48181 ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga
    48241 tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca
    48301 acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta
    48361 gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg
    48421 atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta
    48481 ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct
    48541 agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc
    48601 agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa
    48661 tttctcttga cacacataca atacaattcg accgcaataa tcgacataat aatagctgta
    48721 aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca
    48781 gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca
    48841 gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt
    48901 aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa
    48961 atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa
    49021 aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc
    49081 aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc
    49141 cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa
    49201 tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga
    49261 tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg
    49321 caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca
    49381 taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa
    49441 agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat
    49501 aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac
    49561 tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga
    49621 aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa
    49681 gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt
    49741 tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg
    49801 tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc
    49861 tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg
    49921 cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt
    49981 catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta
    50041 gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa
    50101 atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct
    50161 ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag
    50221 aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag
    50281 gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg
    50341 ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca
    50401 tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa
    50461 tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg
    50521 tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat
    50581 agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa
    50641 tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa
    50701 tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca
    50761 ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt
    50821 aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg
    50881 ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca
    50941 aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct
    51001 ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg
    51061 gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa
    51121 attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt
    51181 gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt
    51241 cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat
    51301 actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc
    51361 ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa
    51421 cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga
    51481 tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc
    51541 caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc
    51601 cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt
    51661 gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa
    51721 aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact
    51781 cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca
    51841 agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga
    51901 tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc
    51961 gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat
    52021 caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca
    52081 tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata
    52141 cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt
    52201 atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat
    52261 catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt
    52321 aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat
    52381 caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta
    52441 agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc
    52501 ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt
    52561 tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg
    52621 cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga
    52681 aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga
    52741 aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac
    52801 tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag
    52861 atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt
    52921 tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt
    52981 cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga
    53041 tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat
    53101 taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac
    53161 cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg
    53221 agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag
    53281 atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc
    53341 ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg
    53401 aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat
    53461 gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt
    53521 gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg
    53581 aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc
    53641 tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat
    53701 acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct
    53761 tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg
    53821 gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata
    53881 cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag
    53941 ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat
    54001 ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt
    54061 aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat
    54121 gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact
    54181 gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa
    54241 aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt
    54301 gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat
    54361 ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa
    54421 taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac
    54481 tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat
    54541 catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg
    54601 aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat
    54661 ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc
    54721 acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa
    54781 tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac
    54841 taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac
    54901 caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc
    54961 tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc
    55021 gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga
    55081 tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc
    55141 aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac
    55201 tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt
    55261 atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt
    55321 ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta
    55381 ttcaattttt tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa
    55441 caagtagcac acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg
    55501 gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa
    55561 cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag
    55621 ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc agctgatatg
    55681 ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt agacctgact
    55741 gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg acttctccgc
    55801 tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt tctttcgtca
    55861 attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc gcatccaatt
    55921 cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct aagccgtcaa
    55981 tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt ccattggcat
    56041 cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg atctttgtca
    56101 acacgacacc gcctgg
//
LOCUS       DAFGFZ010000004        50393 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000004 DAFGFZ010000000
VERSION     DAFGFZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.004"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..374
                     /locus_tag="LR726_000189"
     CDS             <1..374
                     /locus_tag="LR726_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBL6215306.1"
                     /translation="DAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIV
                     PQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKAD
                     YVTVSNDKDGVSQAFERILTI"
     gene            655..1875
                     /locus_tag="LR726_000190"
     CDS             655..1875
                     /locus_tag="LR726_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL6215307.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1872..4592
                     /locus_tag="LR726_000191"
     CDS             1872..4592
                     /locus_tag="LR726_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6215308.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4576..5520
                     /locus_tag="LR726_000192"
     CDS             4576..5520
                     /locus_tag="LR726_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL6215309.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5589..6599)
                     /locus_tag="LR726_000193"
     CDS             complement(5589..6599)
                     /locus_tag="LR726_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL6215310.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6779..7123)
                     /locus_tag="LR726_000194"
     CDS             complement(6779..7123)
                     /locus_tag="LR726_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL6215311.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7116..7550)
                     /locus_tag="LR726_000195"
     CDS             complement(7116..7550)
                     /locus_tag="LR726_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL6215312.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7688..8425
                     /locus_tag="LR726_000196"
     CDS             7688..8425
                     /locus_tag="LR726_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6215313.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8418..9629
                     /locus_tag="LR726_000197"
     CDS             8418..9629
                     /locus_tag="LR726_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215314.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9833..10615
                     /locus_tag="LR726_000198"
     CDS             9833..10615
                     /locus_tag="LR726_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL6215315.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10685..11347
                     /gene="trmB"
                     /locus_tag="LR726_000199"
     CDS             10685..11347
                     /gene="trmB"
                     /locus_tag="LR726_000199"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL6215316.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11445..13199)
                     /gene="ade"
                     /locus_tag="LR726_000200"
     CDS             complement(11445..13199)
                     /gene="ade"
                     /locus_tag="LR726_000200"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL6215317.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13331..14260
                     /gene="tadA"
                     /locus_tag="LR726_000201"
     CDS             13331..14260
                     /gene="tadA"
                     /locus_tag="LR726_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL6215318.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14217..15266
                     /locus_tag="LR726_000202"
     CDS             14217..15266
                     /locus_tag="LR726_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL6215319.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15263..15541
                     /locus_tag="LR726_000203"
     CDS             15263..15541
                     /locus_tag="LR726_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL6215320.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15516..15986
                     /locus_tag="LR726_000204"
     CDS             15516..15986
                     /locus_tag="LR726_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL6215321.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16003..16305
                     /locus_tag="LR726_000205"
     CDS             16003..16305
                     /locus_tag="LR726_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215322.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16322..16693
                     /locus_tag="LR726_000206"
     CDS             16322..16693
                     /locus_tag="LR726_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL6215323.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16665..17024
                     /locus_tag="LR726_000207"
     CDS             16665..17024
                     /locus_tag="LR726_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215324.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17196..18203
                     /locus_tag="LR726_000208"
     CDS             17196..18203
                     /locus_tag="LR726_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL6215325.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18228..19412
                     /locus_tag="LR726_000209"
     CDS             18228..19412
                     /locus_tag="LR726_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL6215326.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19849..21432)
                     /locus_tag="LR726_000210"
     CDS             complement(19849..21432)
                     /locus_tag="LR726_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL6215327.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22010..22153)
                     /locus_tag="LR726_000211"
     CDS             complement(22010..22153)
                     /locus_tag="LR726_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL6215328.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22375..22854)
                     /locus_tag="LR726_000212"
     CDS             complement(22375..22854)
                     /locus_tag="LR726_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL6215329.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22927..23211)
                     /locus_tag="LR726_000213"
     CDS             complement(22927..23211)
                     /locus_tag="LR726_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215330.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23289..23483)
                     /gene="ssrS"
                     /locus_tag="LR726_000214"
     ncRNA           complement(23289..23483)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LR726_000214"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23555..24841)
                     /locus_tag="LR726_000215"
     CDS             complement(23555..24841)
                     /locus_tag="LR726_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL6215331.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24979..25650
                     /locus_tag="LR726_000216"
     CDS             24979..25650
                     /locus_tag="LR726_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL6215332.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25616..26104
                     /locus_tag="LR726_000217"
     CDS             25616..26104
                     /locus_tag="LR726_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL6215333.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26117..27064
                     /gene="prmA"
                     /locus_tag="LR726_000218"
     CDS             26117..27064
                     /gene="prmA"
                     /locus_tag="LR726_000218"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL6215334.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27065..27829
                     /locus_tag="LR726_000219"
     CDS             27065..27829
                     /locus_tag="LR726_000219"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL6215335.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28062..30275
                     /locus_tag="LR726_000220"
     CDS             28062..30275
                     /locus_tag="LR726_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL6215336.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30286..30732
                     /locus_tag="LR726_000221"
     CDS             30286..30732
                     /locus_tag="LR726_000221"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL6215337.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30903..32162
                     /locus_tag="LR726_000222"
     CDS             30903..32162
                     /locus_tag="LR726_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL6215338.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32251..33198)
                     /locus_tag="LR726_000223"
     CDS             complement(32251..33198)
                     /locus_tag="LR726_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL6215339.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33592..34884
                     /locus_tag="LR726_000224"
     CDS             33592..34884
                     /locus_tag="LR726_000224"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL6215340.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34899..36668
                     /gene="aspS"
                     /locus_tag="LR726_000225"
     CDS             34899..36668
                     /gene="aspS"
                     /locus_tag="LR726_000225"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL6215341.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36777..37652
                     /locus_tag="LR726_000226"
     CDS             36777..37652
                     /locus_tag="LR726_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL6215342.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37918..39801
                     /locus_tag="LR726_000227"
     CDS             37918..39801
                     /locus_tag="LR726_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL6215343.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39876..40160)
                     /locus_tag="LR726_000228"
     CDS             complement(39876..40160)
                     /locus_tag="LR726_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL6215344.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40150..40884)
                     /locus_tag="LR726_000229"
     CDS             complement(40150..40884)
                     /locus_tag="LR726_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL6215345.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41013..41636
                     /locus_tag="LR726_000230"
     CDS             41013..41636
                     /locus_tag="LR726_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL6215346.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41828..42949
                     /locus_tag="LR726_000231"
     CDS             41828..42949
                     /locus_tag="LR726_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL6215347.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42956..46084
                     /locus_tag="LR726_000232"
     CDS             42956..46084
                     /locus_tag="LR726_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL6215348.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46103..49297
                     /locus_tag="LR726_000233"
     CDS             46103..49297
                     /locus_tag="LR726_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL6215349.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49443..50150)
                     /locus_tag="LR726_000234"
     CDS             complement(49443..50150)
                     /locus_tag="LR726_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL6215350.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16448 a         8858 c        10503 g        14584 t
ORIGIN      
        1 aggatgctat cagcatttac aaatttttta tttttattcc taatcgtcaa ttgaaagcta
       61 atatatttaa ccatttgaaa aagatgctaa atgtgtctgt tactgaatcg gcacctgtaa
      121 atattgaaat cgtacctcaa aatgtatcta aaggtgtggt gtttgatcgt ttggaatcaa
      181 tctacaattt aaagaaacct ttgagaatcg cgatcggaga tagtttaaat gacttatcta
      241 tgtttgaaag tgcagatatt agttttgcaa tgggaaatag tcatcaagaa atcatgaaaa
      301 aagctgatta tgtcactgtt tcaaatgata aagacggagt atctcaagca tttgaaagaa
      361 ttcttaccat ataaactaat taaggtagta tagaacatag aattctaaat acggaaaaat
      421 ggagcataaa ttccagtaca aatagtactt cttcagtgtc aaacgaagcg aatataacat
      481 agatatttag gattgtgaca aaaatcatgt cacaatcttt ttttcgaata aacttatttt
      541 ttgaagtcgg ccatttatcg taatttcttt gcataatacg aacaaatttt cggttattga
      601 tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa ggaggaaatt tttaatggtg
      661 aaatttatcc atgcggcaga tttacatatg gatcggtcat ttgaaggact gacaactctt
      721 gataaaactg tacaggaaaa actattgaag acgaatctga ccgttttatc aaatatcatt
      781 gaacaggcaa taataaataa cgttgatttt gttttacttg caggagataa ttttcatcaa
      841 aatcgtcctt cattaaaaat acagaaacat ttttcagaac agatgaaacg gttagaaaag
      901 aaccatatac ctgtatttat aatattcgga aatcatgatt tctatcaaaa agagcggtat
      961 tggttctcct ttcctaaaaa tgtccattta tttacatccg aaacggtaga gacaaaaaaa
     1021 ataacgataa aaagtggaga aactgtctct ctcagtggat ttagttatcg tcaaccgtgg
     1081 attcaaaaag acaaagtgat ggaatttcct tctagagaac tcacagatta ccatattggg
     1141 ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact atgctccttt ccaaccctca
     1201 aagatgcaag aaaagggata cgattattgg gctttagggc atatccatgt gccaacggtc
     1261 ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc aaggtcatac taaaaaggaa
     1321 caagcttcta catctattct actagttgaa ttaagtaaag gaagttgcca aatacatccg
     1381 attaaagtag cagaggtcta ttggaagaca aaggaaatat cattgaggaa agcaagaacg
     1441 acaaaagagg tcattgttca cattcgtcag cagctttcaa aagtagagga tggatttagt
     1501 ttaattgaga taaaattaaa agattatgac catctcaata cagatgtttt ggaacggata
     1561 caatcaggag agctattaga ctatttgtta gaggaattct cagataggat aaatgatttc
     1621 tttatttggc gtatttctct catagagaat actttttata cgaagacccc acttgctgct
     1681 tctgctaaat tgatggaaca gttatttcaa tactatcaga cacctcaaga tttccaacaa
     1741 attttaaatg aagtatatag tcatcaagaa gcggctagaa tattgagcga actgccggaa
     1801 tatcaagaag aaacacttga aaaagccaag cagctgctta atcaagattt tttatttgag
     1861 gaggatcagg aatgaagatt ttaaggatcg agattgctgc ttttggaaaa tggcgtcaaa
     1921 aaagttttga cttttattca ggcaatcagc tgatctatgg gggaaacgaa gcaggaaaat
     1981 caacgattta tcaatttatt caagcaatat tatttggttt cccttcaaaa ggccgtaaaa
     2041 aaaaggatta tacaccaaaa gacggctcag cctatggcgg caaaatatgg cttaaacatc
     2101 ctgtatatgg tgaatttgct gtcgagcgct acaagcagca aaatcgcgga aaatcgaagg
     2161 tgtggctggg ggatcaagtg ggaagtgatg agttactaga aaaactgatc gcacctttga
     2221 cgaaagagtt attccagcag gtatttactt tccagcaaga gcagttgatg gaactagatc
     2281 atttgagaga aaaagagcta catgatgcgc tgatttcttt aggcatcacg ggcagttctc
     2341 aagtattcca aaagcggcaa gaatattatc agaaagcaca acaattattc aagaaaaaag
     2401 gccaaaaatt actaatcaat caaaaattaa atgaatggaa agagctgcaa gaaaaaattc
     2461 atcaaaaaca agaacaggaa gcttattttg gcaagttgat ccaacaagaa caggaatacg
     2521 agtcgcaaca gcagcagtta ttggcacaat taaaagaggc gaacaaacaa ttttcattag
     2581 ccagacagca agaacttaat ttttctttat atgaagaatg gcaatctctc aaaaaaaaca
     2641 gcactacagg tcttcctgat gagcaacttc ttttagatct ggagtctttc tccaaacggt
     2701 atcagcaaac aaatgagcgt ttggaagaat tagaagcaac actggaaaag catagcggga
     2761 tggatcagca gtcagccaga tattattttt atttagagca ggagcaggaa ctaaaagatc
     2821 tccagcaact tcaatttaca gcggaacaat taaacgaaga atggcaaaga ttacaaattg
     2881 agactagaga aacaaattca gaattgaaga ttttggaaca aaggtggcat tggcagaaaa
     2941 atccgttacc ggaagaattt ttatatgaag aagaatggaa gcatcttctg gctgaagaaa
     3001 gaaggattgc tgatctagaa agccagattt ctgtgcgtct ccaaatagct aaagaacagc
     3061 aagagatgat tgaagcagaa gtgaatgact tggaagagac ctatccagca ttattacaaa
     3121 atcagtcaga gatagcacca gtctcttcat ctaaaccgta tatgctgatg gctggcggag
     3181 caataatcat tgcaggtata tttgtaccaa tgcccttgaa agtgatcctt ttactaattg
     3241 gtgcaggcat gattggctat ttcttctaca aaaggcaacc aaaaattaaa actaaaaatg
     3301 atttctctga gataaaagga atgtggcaag aaaaactagg acaacttgat ctagccttag
     3361 ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg agaagaaaaa cagattcttc
     3421 agcaagaagt atatcgtttt gcggatcatc ggcacttagg aaagatggat acgctcaaac
     3481 tcatgaaaga atatggacag gaagtcatag attatcagaa attatatggc gtatttgagc
     3541 ggaaacataa aagagaacag gaaatacaag aaaaactacg acaaatcgac caaaaatttg
     3601 agtttgttaa agaatggtta ccgatacaag ataagacaat ttcagaaaaa atggtattgc
     3661 tttcccgttt tatacaagag atggaacaga tgaaatttgc cagaagctat cagcaaaata
     3721 ctttattgaa gcaagaaatc aatcaattga aaaagaaaca aaaagtatgg atacaagaat
     3781 acagcgaaca attgactcaa gcaggcttat cttatccttc ggaagtacct gtctttttac
     3841 aaaaagccaa acaagccgaa cagcagatga aaagatacaa tgaattatcg gaaatgctta
     3901 ctccactatt tcctgaagag ctgacaaaag agaaattatc tgtaagactg cgccaactga
     3961 aagaaaagca atcccaactc cagcaagaag aaaaagaagc aagtgaaaat cggcaacgac
     4021 tccaacttca agtagaagag ttgcaaagag acggcacatt ggacgaactt tatcaacaag
     4081 ctagccggca aaaagcaaaa ttacaggaat tactggagga atggggagca cttgaaatcg
     4141 ccgggacatt cttaggtgat ctttcaacag agatgagtga aagacaatta ccccagttgt
     4201 taagagcagc aggtgattat ttaggtattt taacaaaagg aaattatcag aagatattac
     4261 tgaaaaatga tacactgact ttaacagacg gatatacaga atttcccatc tatgagttat
     4321 ctacaggtac gaaagatcag ctgattatgg cgatgagatt tgcttacctc tcattagaag
     4381 ctaaccgttc gatatgcccg atcatcattg atgatggctg gcttcattat gatcatcagc
     4441 gaaagtatca attagctgaa cttttagcta aatttggaga aaagtatcaa ataatttgtt
     4501 tttcttctga taaagaaatg gtaagctatt accatgagat gaaccaaatc gtaaaacaat
     4561 tagaaggagc aaataatgaa aaaaattcgt gatttagtaa tcgatgagca gtttgaacaa
     4621 tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa acggaaaaaa atttattgct
     4681 tttactttcc aagatacttc aggtactatc gatggtaaat actgggatgc ttcggaggag
     4741 gaaatcaata aatttacagc cggcacagtc gtttttctaa acggtaaaag agaggtttat
     4801 caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa caagggtaga tgagccagga
     4861 gagcctagtt tatacatgga aagagcccct ttgaagaaag aagaaatgga agaagaaatc
     4921 aatcaattgt tgtttgagat caccaatgcg cattggaatc gtattgttcg ctatctactt
     4981 aataaatatc aaaaacaatt ttttgagttt ccggcagcaa aaagaaacca tcatgcattt
     5041 gccagtggat tagcttatca tactttgaca atgcttcact tagggaaatc aatcgccgat
     5101 gaatatcctg agctgaatcg agcattgttg tatgctggta tcattttgca tgatttagga
     5161 aaggtgattg aactttccgg tcctatgtcg acagaatata cgcttgcagg aaatttgatc
     5221 ggtcatctgg tattgattga cgaagaaata actaaagcct gtcttgcgtt gaaaatcagc
     5281 gaagctgacg aagatgttct cgtattacgt catatggttt tagcacacca tggcttatta
     5341 gaatatggct cacctgtacg tccaagaata atggaagcag aaatattaca tcaaatcgac
     5401 aatatcgatg cttcgatgca gatgatgttg acttcgattc gtcaaacaga accaggaaaa
     5461 tacaccgatc ggattttcgg tatggacaac cgaagtttct atgtaccaaa agatttatag
     5521 caaaaattta tttttttcat tcaaaacaaa aaaagcgaga cataagtctt cgcttttttt
     5581 gttttctttt attttgatga atcagttgtt tcttcagttg aagattcttt tgttgaatca
     5641 gttgattttg tgtcagaact ctttgttgaa gctgtagttt cacttgagtc tttcgtactt
     5701 gatgaagttg ttgtgaatgc actcaacaca ttttcaaagg catcatcttt gatttttaca
     5761 tttgcatctt ttaattcttc accgattact ttagttgtga atgcattgtc acttagttta
     5821 gtttcagtag cgatatcttt tagttgatct ttgtatttat ccatgtcatt gcctttattt
     5881 tggtttttca ccattttgac aacgtagtat tctgtcgcat aagaagtagg atttgttgta
     5941 gtaataacat cggagatctc accgtctttc aatttgaaag ctgcttcttt tacttctgca
     6001 ggaatagttg ttgtcgttga gtcgaattta actttaccgc catcttcttt tgtctctgta
     6061 tcagttgatt tgtctttggc taatttagag aaatcatctc catcatccgc tgattttttc
     6121 acatcttttg cttcatcttc acttgataat ttgatgattt gtgcttctac ttctggatgg
     6181 aaagatttcc aagcagtttt caaatcgtca tctgtaatat ctacgtgagc tttcaagcca
     6241 gcttcaaaag ctaaattgtt acgaatatat tctttataag tatctttcgt atatccagca
     6301 gcttctaatt gtgattcaaa agtatcacct aatgatttcg cttgtttgtc gtattctgca
     6361 tccacttgct tatcggttac tttatcgcca tatttgttat tgaatacttt ataaatgatc
     6421 atccgttgta ctaaggattg gttcgatgat tcaagttttg cttcatcata gaaatcagat
     6481 acagtgatcg ttccgccttt catcgttgcg atatctttat tagtatcgcc tgaacaagct
     6541 gctagagtta aaacagccaa cgcactagta gctgccaaaa taattgattt ttttttcatt
     6601 aaaatttgca ctcctgtaat tagttattat ttttatatat taactatacc atatcccatt
     6661 tcctttaaaa acgttgtcgt ggtagtttca aacaaaattc aaaatttcat cacattccta
     6721 ggaaatacgc aaacttttct cacttatata gttaaatctg ttgagaaaat aatcattatt
     6781 atatttgttc aggatactct ggcagatcag cttttagttt ttctacttgt tcattgattt
     6841 gtgtgatacg tggttcggct tggaatttaa aatcttcgat atcttttttg attgcttctt
     6901 ggaaagcagg aatcacagat tcagctgttt cttttgtttc gatcaatgcg cttttaaaat
     6961 gattcaggct gttatttaaa tcatttgtca gagttgttgc ttcttccaat tcttcgatca
     7021 gtttctttct tgtttcattc ccgctcctgg gcgccaataa cagacctccg acagtgccag
     7081 cagcagctcc aaataacagt cctttgaaaa agtttttaat catcgcagga cacctgcttt
     7141 acgattgttt cttgaatagc ttttagttct tccgatccat aactatcttg atgattgaca
     7201 aagtgaatag aaaaatcatc ctctttgctg tagcgaggga ctaaatggat atgtgaatga
     7261 aaaacagatt gataggctag ttcacggttg ttattcaaga tattaagacc tttcatttct
     7321 ggaaaagctt tttccaatgc ttgagcaatc ttaggcacac gcgaaaaaac ctctcctgca
     7381 agttcgggat catattcaaa aatatctgct acatgacgtt taggcacaac taatgtgtgt
     7441 cctttcgttg cttgagagat gtctaaaaaa gcatagacct tgtcgtcttc atatatttta
     7501 tagcttggga tttcttgatt gatgattttg caaaaaatac agttttccat ataaaacatc
     7561 ctttctataa agaataaata cttcagctta actttaccat aagttaaagc tattgtaagg
     7621 aaaaaaacaa aataccgtca ttttcttcgt ggtataataa ataaaaataa atggcggagg
     7681 aaaaagaatg agtttaatta tcgatcatgt cacagggggg tacggacatc tccctgtgtt
     7741 aaaagatatc agtttcgatg tacagcctgg agagatagta gggttgattg gactgaacgg
     7801 agcagggaaa agtacgacca tcaagcacat tatcggtctg ctggaagctt caaaaggaac
     7861 gatagaaatc aatcatctat ccttaaagaa aaatacggaa gaatatcgca aacaaattgg
     7921 gtatattcca gagactcctt ctttatatga agaattgaca ttgagagaac atatcgaagt
     7981 cactgcgatg gcctacgata tacctatgga tatagcaatg aaaagagcag aaaagttgtt
     8041 ggagactttt cggttatcta atcgtcttga ctggttccca gctaattttt caaagggaat
     8101 gaaacagaaa gtgatgatct tatgcgcctt tttgatcgag ccttctttat atatcatcga
     8161 cgaaccattt ttaggcttgg atccactagc tatcaatgca ttgcttgaat tgatggtaga
     8221 aatgaaaaaa gaaggagccg gtattttgat gtctacgcat attttagcta ctgcagaaaa
     8281 atattgtgat cgttttgtgg tcttacataa cggagagata cgagcagacg gttctcttga
     8341 gatgttgaga aaagaatttc agctgccgga ttcttctttg gacgaaatct atattgcttt
     8401 aacaaaggag caggcacatg agtagttttt ttcaaaaacg tttagcaaga catcaaaaga
     8461 aaatgatgaa atatatgcgt tatgtattga acgatcattt tgttttagtt tgtcttttct
     8521 tgattggcgg tgtgggatta tattattcta attggttgaa aaccctgcag ccaccgtttg
     8581 caattggagg gctcataatc tctgtatttt ggatgctctg tttattcgtg ggtaagatgg
     8641 ctacttttgc agaggcagca gatattgttt ttttacttcc taaagaaaaa gaaatgcaaa
     8701 cctatttgga acgagctttt cgctattctt gcatttttcc gttgatcttt ttgatattgg
     8761 catgcggatt ggccatgcca ttagtggtgg ttagtacgga gcaatccttt tcgatgtttt
     8821 tcgtctatct gatcatatta tggagtttga aattcagtca tctgcaaatc aagagagcag
     8881 aattgtttcg actagatcaa caagttattc gttattggaa attaggctgg tttttttctt
     8941 ctctgatctt tttgtttcta tcattttatt ggctaatatg ggcaggaata gcaggtgcat
     9001 tagtacaggc aagtctatat tactggtttt tgtggaagca acaagaaaaa ggattggatt
     9061 gggagaaaat ggtgcatgta gaagaacata ggttgcatca gatctatcaa tttatcaatt
     9121 tattcacaga tgttcctgaa attacggcaa agatcaaaag aagacgctat ttggatccta
     9181 tattgactca tattaaaaga gaatcaaaaa acacttatct ttacttgttt gcaagaagat
     9241 tggcaagagg ttctgatttt agtggtcttt atttccgatt agtaattata ggtgggttat
     9301 tgattgccgg agtagtcgac ttttatttta gtctgattat cggtttctta tttttatatt
     9361 tgatcggttt ccaactgtta cctttagcga accagttccg ctatatggtt ctcgttcagc
     9421 tttatccaat ccataagaat cagaagaagc aggcgattca gtatctactc acatgggtat
     9481 taattatcgc gtcggttatt tttggtttga ttgctgcagt cgtattagat gggcagcagc
     9541 gatgggttcc gctagcggct tatttgattg ttactatgct ctttgttaaa ctatatgtac
     9601 caactcgttt gaagaagatg tcagactgaa aacaaagctg tgtagggtaa aaatgagaat
     9661 gaaaaataga gcgttgacag taagaatgct gttcgttcta ttttttttga gaaaagctgg
     9721 ttttttgaat ggcaattaaa tgaaaaagaa cgtatatgtg cttgacgtgt agtgttcagg
     9781 cgactagaat aataagtact gcaaaacaaa gagtaagagg acgggagaat gcatgatgga
     9841 gtttcagtta gaccaagagt ggcggttacg tcccatcaaa ggcgacacag gaaaaacata
     9901 cataggcatg cgtgatgagg acaaagtctt tattaaacga aacacgactc cgatgttggc
     9961 tgctctttca agggaaggaa ttacaccaaa acttgtttgg acaaaacgaa cggggaatgg
    10021 cgatactttg acagcacaag agtggctgga aggtcgcttg ctgactccta atgagattgg
    10081 tcaaagaaac gacgtgattg atgttttgta ccatttacat cattcaaatt ctcttaaaac
    10141 catgctgacc cgtatcggtg gtcgtgtcat gacgccggag aagatgctgc aaaaatatga
    10201 aaaagaatta ccggaagaac tccagaaaaa tcaatttctg caaattgtcc atcgttttct
    10261 tcgtgagcag atcccgtcaa tagataagga aaacttatca gtagtacacg gagatgtaaa
    10321 ccatcgtaat tggttagtat gtaaaaatta tttatatcta gtcgattggg attcaatcat
    10381 gtttgcagac cctgcggtgg atattgcaac gattttagga aattatgtgc cactgtctag
    10441 ctggaatcag tggctgatca gttatggcat acgcccgact gacgaaatgc ttgaaaaatt
    10501 tcattggtat gcggccatga atttgctcca agaaatcacc agatattgca aacgtgaaga
    10561 tcatcgacgg atgaacgaag aaattttaca attaaaacga attttcagtg gctgaggttt
    10621 ggcccaaact ctgcggagat ttggccagac ctttttaatt agaggaaagg aatggagata
    10681 aataatgcgt gtccgaaatc gtccgggagc agcggaaatg ttagcggctc acccgaattt
    10741 tgtaattagt gatccaacat tgtggaaggg aaaatggaat gaattgtttg aaaacgatca
    10801 tccaatccac attgagattg gtatggggaa aggccagttc atcactggaa tggcaaaagc
    10861 ccatcctgaa atcaattata ttggagtaga aatgcaggtc agtgttgttt cgattgcact
    10921 agacaagttg atcgaacaac cgcttcctaa cctgaaactt ctacatgtgg atggatcagc
    10981 gttgacagag tatttcgctg acagcgaagt agatcaaatc tatctgaatt tttccgatcc
    11041 atggccaaaa aaacgtcatg aaaaacggcg attgacatat aagacttttc ttgctgtaga
    11101 tgaacagatt cttcgtccaa atggagaaat ccatttcaaa acagataatc aaggattgtt
    11161 tgagtattct ctggcaagtt tttctcaata tggcatgatc ctcaaacagg tatggttaga
    11221 tttacatcaa agtcaatttg aaggcaatat tatgacggag tatgaagaaa aattttcttc
    11281 aaaaggtcaa cgtatttatc gagtagaagc tcaatttcaa gataagacca aaaagaaaag
    11341 tctgtgaaac taactttctg ctttcataaa aacattaata tccataaata aatctcagaa
    11401 aatgaacatt acagatgtaa tgtattttct gggatttatt tgatttaaaa tgtatttatt
    11461 tcatcggttt ttccacaagg tggaatcaat tggaagcgag aaaatgaaaa taatcctttg
    11521 tcggtgatct tcaattcggg tatgacagat aatgttaaga aagaaagtgt caaaaaagga
    11581 tcaaaggttt tagctgctcc tagttgatgt gctttagtta ttaattcatt caactgttca
    11641 tagacgatcg aaaaaggttc ctgtgtcatg attccgccta taggaagaga aacacaagct
    11701 atttcctgct gtttgttgac aacggtcatc ccgccacctt tttgaatcaa aagattagca
    11761 gcatacagca tatcctcgtc gtttgtccca acgacaacta gattatgcga gtcatgggca
    11821 atcgttgtcg ctatcgctcc ttgtttcagc tgaaatcctt tcactatgcc taatccaatg
    11881 tttcccgttt gatggtgacg ctcgatcacc gcaatcttta attggtcttt ttcgattgaa
    11941 ggaatgaatc gttcgccttg tcggtccact ttttctacca aatctttagt cactagacta
    12001 ttcgggataa tttcgatcac atgggcttgt gaagaagaaa gaagcagctc taacgaatgt
    12061 ggatttaatg gttgagcgtt tagttttggc agacaacacg tatatgaatt tgaatgatca
    12121 gcaaataaat ggtcttggag ttgcccagat tgaacaacta atgttccctt atagaaaact
    12181 tcatgaattg gcagcttggt aagatcgtct gttaagaaga aatcagcttg atacccaggg
    12241 gcaatcgcac ctagtgtttt caaatcaaaa cattctgctg cattcaatgt tgccatctga
    12301 atggcttgta atggatcgat tccaagtgca atagcttgac ggacgatatg atcgattgat
    12361 ccttctttta ctaaatcatt gatcagttta tcatcggtga caaagaagca tcttcttgaa
    12421 ttttcaggtg tgatcgccgg tagtagcgat gccagatctt ttgcaacggt cccttctcga
    12481 accattaaat acataccaag atccaaacgt tctttagctt cttttgcagt tgtagcttca
    12541 tgatccgtcc gaatacctgc tgcaggataa acatttaact cttccatgcc gataccagct
    12601 gcatgcccgt caattttttt cttatgctgg tgcatcaatt ggatcttttc taggatgtct
    12661 gtttcattat ttgctactgc ttgatagttc atgacttcag ctaagcctaa aacttcgggt
    12721 ttttgtaaaa aaggggctaa tttttttgca tttaaatttg caccattggt ctcaaaaggg
    12781 gttacaggta cacaagaagg tagcataaaa aagatattca tgagtgtttg tcttgcatct
    12841 tctatcataa actcaagacc tgtactccca gcaatgtttc ctatttcatg gggatctgtc
    12901 actacagtag taactccatg aagcaaggat acttttgaaa attcgcttgg tgataaaagt
    12961 gagctttcga tatgcacatg gccatcgatg aatcctggca ctagatattg cccattagca
    13021 ttatgtacgc tttttccttc gtattcaccg attcctacga tcattccttc gcagattgct
    13081 acatcttttt ccatgatttc tttcgtaaag acgttgacga tcttagcatg cttgatcact
    13141 aggtcagcag gctttttttt acttgctact tcgatatatt tccttaatgt tgtttccaat
    13201 ggagtgcctc ccaagataaa tttttataag tataacataa attcggcttt tgccaatatt
    13261 tttttgtaaa ttcattttat ttgaaacaaa aagatcaaaa agacgtatgt tttttgtgga
    13321 ggggagaata atggaaataa aacgattatc ggatcaaatg ataagtgaaa gttttgccaa
    13381 acatgcccaa gacgtatata tattgccagc ttcagaagta ttttacatct attttcgcta
    13441 tggaaaaaaa cgaacattct ggaagaaagt ctcttcggca gatggacagc aactgatctc
    13501 aagatttaag tatttgggac agatggatat cggagaaaaa agaaaagctc agttaggtgc
    13561 atttacttat tctttagaaa aacaggaagt tcgtctgcgg ttgtcttctg taggagatta
    13621 ccttcaacga gagagtttag tgattcggct attatacggt acttttgaaa caccccatcg
    13681 ttgctttagt tctcgagatt ttgcaactat tttacagaaa accgtcagac gtggattata
    13741 ccttttcagc ggaccagtcg gatcaggcaa gacttctcta atgtatcggt tggctttgga
    13801 agaaaaattg caagtgatta ctatcgaaga tcctgtagag atagaagaac cgttttttct
    13861 gcagctacag atcaatccga aaatagcaca gacttatgat acacttttga aactcgcatt
    13921 acggcatcgt ccagacttgc taatcattgg cgagataaga gatgaatcaa ctgcacatgc
    13981 tgcggtaaga gcagcactaa cagggcaccg ggtttttgca accgtacatg cacgcaatct
    14041 tgaaggtaca ttggtcagaa tccatgaact gacaaagaaa aaagaagagc tatctgaatg
    14101 tttagctggt gtcatttatc aagaactttt ggaagatgcc aatcaccatc cttctgtttt
    14161 atggagttat caatttatgg aggaagaatc agtaaaaaaa tcatgggagg aaagttatga
    14221 agaagctcag gaaaatattt ggcagtcaga actattctaa aggattaaca agaagccagc
    14281 aaaaccagtt tgcacaaacc atgggagaac tgcttttatc tggttttagt ctgcaagaat
    14341 ccctttattt attactcacg atccgttcgt ttccacctgt tattttggca aatatccaac
    14401 atcagctaaa ggaagggcaa gcgtttagtg aggtactttc gcaaatagga tatggagcag
    14461 agcaactggt acagatcgag ttagctgaac gtcatgggaa tctgccgaca actttgttgg
    14521 aaattgccaa acagtttcgg cttgttgaag gatttcgcaa tgatttgaaa aagctgcttg
    14581 catatccgat gctattgctc acctttttgc taggtatctt gtttagtatg cggttgttcg
    14641 ttttgcctca gcttattggt tcaggaatgg tcatgcaaga gcattggggt attcagctga
    14701 tacaagtctt tcattggtat gttctactat tcatcctagc ggttactcta ttggctttac
    14761 tagtaaggta tcgtctgatg aagttgtctc ctgttgatca gagtgaatgg ataagcagac
    14821 aattattgat tggaccgata tattccttgt atcaatcggc ttatttgtca ctagagattg
    14881 gaaagctttt tcatgaaggt ttcgaattaa ggcaaatcat agtctgctta aaggaaacga
    14941 aacaagaaag tctgattcag catcttgcct ctaagttgat tcaaggattg gaggcaggta
    15001 tgtcattggc tgagcagttc cagaattatc gttttctatc agaagaattc agtaaaattg
    15061 ttttacaggg agaggcaaag ggaagtttag gtaaagaact gattttttac agcgaatgga
    15121 caagaaaaca gcttttcaaa cgtcttcatc actggatgca ttttattcag ccaatcattt
    15181 ttttgggcgt agcggctttg attttattag tttatgccgc tgttttatta cctgtttacg
    15241 gaagcataga ggaggttttg ccatgaaaaa gagaaaaata tcttattata gcggttttac
    15301 attgatcgaa atgctaatcg tcttactgat catcagtgta ttagtgttgt tgttcgtccc
    15361 taatttgtcg cgttatagga atcatgtaga ccaagaaagt cgtgaagcga tcatccagtt
    15421 ggtagacaca caaaaagagc tctatgcgct tcaaaataat ggaagagttc ctactgtgga
    15481 ggaattgttg aatgaaggct atatcaaacg agaacatgca gaaatctatc agcggccttg
    15541 aaggtaaggt tttagcatca ggatatacgt tgtttgaatc actcttactg attttattac
    15601 ttacaacgat cctttctttt ccagttctcg cattttctgc atggaagaat gaattggcag
    15661 tctatcaatt tttcggccaa tttgaaaaac gaatctattc tacgcaaaaa atcgctattg
    15721 tgaatcagat ccaaacaggc ttttattgga atagtgaaga gaatcaaatc atttttcatg
    15781 ttccgaatcc agataaaata aattgggaag tattagatat tcctgatgag attaccttga
    15841 aacgccatgc ttctattact tttgctgcta gaaccgggaa tgaaagttca ctaaaagctt
    15901 atcaatttta ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg
    15961 gtcgctatac aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg
    16021 cttatgatcg cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag
    16081 gcagaaaaga aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag
    16141 acgcagcgga tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc
    16201 caaattgtcg ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg
    16261 atcagtattg aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact
    16321 gatgatattg agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt
    16381 aagtgaattt ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga
    16441 agaagaactc aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat
    16501 cgaggataaa cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc
    16561 cggcggacat caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca
    16621 cacaatttct ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa
    16681 aaacgatcag tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc
    16741 ttttatgtac gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta
    16801 atatacgaat caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac
    16861 agtctacgct tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat
    16921 atcagtgggt acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt
    16981 tcacagaaga agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa
    17041 acgccctctt ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat
    17101 aaaaaacagt gaaaacgatt gaaattttgt caattgttcg atagaattaa acaggtgaaa
    17161 aaattgttat aatatatcta gaaatgaggt gcaccatgtt accagaaaaa atggaacaag
    17221 ggtttaatca aaacctggaa gctattcagc ttttacagaa tgcattggga acttcctttt
    17281 tagaagccta tgtcgaaaat gcagaaaatt taatagatga ttatcaagta cgtgtggtgg
    17341 atggagtgcc gacaaaagag acaacgcaac ggatcacagc tctttatgaa gagttgaaga
    17401 aactttcttt cgagccagaa gaatggcgca gactgtctca attactttta ttgaagggca
    17461 gccagacaga acatttgcag gcgaaccatc aactcactcc tgatagtatc ggttttttat
    17521 ttgtttttct tattgaacaa ctatacacaa ataaaaaagc accggttaag attctggata
    17581 ttgcggcagg aatgggcaac ttgttattaa cagttctttt aaacctatcg aatgcggggt
    17641 accaaacaga aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta
    17701 caagtgattt gacacaagca aatgttcaat actttcatca agacgggttg caggaattat
    17761 taatagatcc tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg
    17821 aaaaagcaaa agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat
    17881 tgctagagca atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga
    17941 gtggattttt agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt
    18001 atcttcaagg aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa
    18061 gtattttgat cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg
    18121 tgaaacttgc ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa
    18181 actggaaatc atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa
    18241 ttgcaatcaa tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa
    18301 ccgtagttgc gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca
    18361 agtacggcga taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag
    18421 taaaaatgct gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca
    18481 ccggagtagg gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg
    18541 atgagaaagt aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg
    18601 caaacttaat ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg
    18661 tattcgatac atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg
    18721 tgtcttatta tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt
    18781 atgtgtcgga acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca
    18841 cttgccactt aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata
    18901 cttctatggg attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg
    18961 atccagctgt tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca
    19021 atgttttgaa caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg
    19081 atcttgaaaa gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc
    19141 gcatccgaaa atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct
    19201 ttactgctgg tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt
    19261 cttggtttgg ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata
    19321 tctctacaaa agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga
    19381 ttgcccgtga tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg
    19441 aaacgcatgc aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg
    19501 taatttaact tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa
    19561 aagtggtcaa aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata
    19621 ttttattttc attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta
    19681 gatcagcctc gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc
    19741 cgaaacattc attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc
    19801 gtgaaagcac aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag
    19861 ttggccgagc ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg
    19921 ttgctgcaaa aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag
    19981 catttcttca atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat
    20041 caggacgttg attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc
    20101 ggataaatct ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg
    20161 ccgtcgagcg acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc
    20221 gggatcggca aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt
    20281 gatggtgcca ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc
    20341 tgcaccattg tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc
    20401 tttgaatagt acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt
    20461 gccagatttt ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt
    20521 gacgagtgtc agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc
    20581 attgtctcct tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac
    20641 ttctttttgc tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac
    20701 tcgaaaacgg ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt
    20761 ttttgtggtg acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt
    20821 tgtattctgt aaatagtttt ccaaccagcg aatactgtta agatctaaat gattggtcgg
    20881 ttcatcgagt aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac
    20941 tcgttcgcct ccgctaagtt ctgttaaccg tgcttcctga cggtgtggaa tccctaagcc
    21001 ttgcaagatc gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg
    21061 ctgcagctca ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa
    21121 tgcttcgtag cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa
    21181 gtagtggttg acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa
    21241 gccttttttg cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa
    21301 aaaggttgat ttgccagttc cgttgatacc gactaaggca attttttcac cagcttcaat
    21361 ttgacaggat aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc
    21421 ttgtatgatc atgatctctc tccttgagac cactcaaaaa ggccatggtt atccctggcc
    21481 tgtgttattt aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac
    21541 gtgaattcaa ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca
    21601 tggctaaatg cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc
    21661 atcatagtat tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga
    21721 aagttcaact agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc
    21781 tttttttatc tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac
    21841 cagcgttcag gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt
    21901 tgccatttgc ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat
    21961 cgcgttcagg agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga
    22021 atgatttacc gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta
    22081 gtttggcgat aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat
    22141 cggaaacttc cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac
    22201 accagggcga ctaatcgccc tggtgttcac tttttttatt ttacttgaac tcattgactg
    22261 ttttcccttt attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt
    22321 atcacggttt tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc
    22381 tttggtgtgg tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc
    22441 gcaagctgcg tttctaataa catattcaga aacagaacca ataaataaac gttctacagc
    22501 attcaatcct gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct
    22561 ggcaataatt tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc
    22621 tttcttcgca ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc
    22681 tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc
    22741 taatagaagt tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc
    22801 agcttcatca gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat
    22861 tcactcctca agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag
    22921 aattatttac tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga
    22981 aacaataatt acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt
    23041 taaaataccc attacgccta aaacagcata tgcagcaccg taaaattgta ggaatccttg
    23101 atttttatca tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata
    23161 gctaacgaat aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc
    23221 ctccaaaata agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt
    23281 aaaagatggt aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc
    23341 aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc
    23401 agtgacacaa gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga
    23461 caattcgaac atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat
    23521 gagagagctc gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc
    23581 actcttttat acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg
    23641 gctgataata ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct
    23701 gatcaaaatg atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt
    23761 aatggctatc tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa
    23821 gtgctgcatc taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga
    23881 cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag
    23941 cgttcactgt acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa
    24001 ccatcagacg ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat
    24061 gaagtgcagc atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat
    24121 aatgagcatc tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga
    24181 tcgacagtgt aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt
    24241 ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag
    24301 catcttcgtc taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat
    24361 gttcgactgc caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac
    24421 ggctgcggat cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga
    24481 tgattcggcc actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt
    24541 gtacttcatc caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt
    24601 gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc
    24661 cggcgatcgc acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg
    24721 aagagagcat gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct
    24781 gctgaccaac gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca
    24841 taggattttc ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc
    24901 tagaaagaac agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg
    24961 ataaagtagg tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa
    25021 acgacgccag aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa
    25081 ggaagattag ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc
    25141 gcccatagct atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt
    25201 acgatctacc tttacacgat gcatactcat cggattttga acatcattac tcagccagaa
    25261 gggattcctc aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg
    25321 agtaaaaatc gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg
    25381 gaagcattag caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc
    25441 ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc
    25501 ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat
    25561 atctctttac aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa
    25621 aaagaaggat ctattaacat atttagatga aataattgaa aaacaagtga cagactatga
    25681 tgtagcttta gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc
    25741 agaaaataaa gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat
    25801 tatcgaattt gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg
    25861 caattatcta gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga
    25921 ttctttagtg gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt
    25981 tttggatgaa gacgatgacg aagctgtatt cgagcttcac tgggataatc aacaactagc
    26041 agaattagta gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta
    26101 ttaaggagtg acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt
    26161 cgaagcagtt gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc
    26221 tttagatgtt gaacattttc agtcggatca atatggtgag attttagata aaaatgattt
    26281 tacatcgctg aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc
    26341 ggaaatcttg ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat
    26401 tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa
    26461 gaaatactat catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa
    26521 ctaccagaca gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg
    26581 cacaggcact catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg
    26641 tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata
    26701 tcttggtgca aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa
    26761 agaaaatatg gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt
    26821 gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt
    26881 attgatgatc ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg
    26941 aatcattgaa gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt
    27001 ggatcaaatt ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga
    27061 atagatgcag cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga
    27121 aggggaaaac tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt
    27181 tttggcattc aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc
    27241 tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac
    27301 catagcgagt ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga
    27361 attaggagcg caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc
    27421 aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca
    27481 atcccatcgg cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga
    27541 gcggctagat gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga
    27601 aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt
    27661 ctttggacca gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg
    27721 gatactttgt ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt
    27781 aagtgctgct agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt
    27841 aataaaagag tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac
    27901 aattatttat acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt
    27961 ccgtaaaaaa acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc
    28021 gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt
    28081 taacgggacc cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat
    28141 ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag
    28201 gcgaacccta cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg
    28261 atccccatac agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt
    28321 tagaagatct gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca
    28381 aattagggaa aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga
    28441 tgctgctagc aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac
    28501 ataacatgcg tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa
    28561 ctttagaaat ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat
    28621 tagaagatac agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga
    28681 tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa
    28741 tagcgacaga agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt
    28801 caatctatcg taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag
    28861 ctattcgagt gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca
    28921 caaaatggaa accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata
    28981 tgtaccaatc tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa
    29041 tccgaacaca ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata
    29101 aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc
    29161 atgagatact cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa
    29221 aaggagatat ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt
    29281 taccaaaagg atccggacca ttggactttg catacagtat ccatacagat atcggtaaca
    29341 aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg
    29401 gagatatcat tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga
    29461 aattagttgc tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc
    29521 gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat
    29581 ttacgcctaa agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt
    29641 atcaaacaga agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga
    29701 tggccaatcg tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag
    29761 aagcagaaga aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg
    29821 aaggtggcgt tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta
    29881 atccgattcc tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc
    29941 atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg
    30001 aagtcgaatg ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt
    30061 acggctataa tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga
    30121 aaaatctcaa cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga
    30181 cggttggtat ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac
    30241 ctgatgtcta cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt
    30301 caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac
    30361 ggcctacttg ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg
    30421 gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt
    30481 gtagaagatg tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg
    30541 aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta
    30601 tacgaatcat tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc
    30661 ggagcagata tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct
    30721 aaaaacaaat aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt
    30781 tagactgata aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca
    30841 gcagtcggaa aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga
    30901 agatgaaaga agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa
    30961 aaggatacaa catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa
    31021 agattttcaa taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg
    31081 atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg
    31141 gtcaaggtgg agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta
    31201 tggatctaat gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga
    31261 cacaagcact tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt
    31321 tctttacatc tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg
    31381 tcgaaaaatt agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg
    31441 atcacataaa aggtgtcgag aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg
    31501 aagaagctcg acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca
    31561 atgccacaga tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga
    31621 acatcatccg tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt
    31681 tcagtagacc agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt
    31741 ttattcaaga aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga
    31801 aagaagtaga agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat
    31861 atggaacaga ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt
    31921 atgtagggaa agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg
    31981 ggggaaattt tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac
    32041 gtgtgttttc cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga
    32101 cgattccatt caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt
    32161 agattagcat aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc
    32221 atattactat aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt
    32281 ttgataatct tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc
    32341 tggacgctta gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa
    32401 aacaagttgc gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc
    32461 ggttaaaatt tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc
    32521 ttcaccaaat tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag
    32581 aggaaaatag ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc
    32641 agcctgccga tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt
    32701 cgcttctttc aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc
    32761 ttcttttaat tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa
    32821 gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga
    32881 ttgtttgaga aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc
    32941 ttttgtagcg atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat
    33001 cagttcttcg gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct
    33061 attcaaagct gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt
    33121 atgtcctcca acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc
    33181 gattcttatt tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa
    33241 aaagctgaac taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac
    33301 tggtttttag taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga
    33361 aactttacaa gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga
    33421 cactctggag atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa
    33481 agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga
    33541 tgaacaaggg tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc
    33601 caaagaccaa aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc
    33661 gaagaaacag cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt
    33721 tttgaacact atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa
    33781 gagatgtatg atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca
    33841 gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg
    33901 ttcaaaactt tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta
    33961 cgtcaattcc atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg
    34021 gagagcatgg tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta
    34081 gtcatcaatt cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac
    34141 tatcttttac cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat
    34201 ccattacgcg tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca
    34261 tctatcttag attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg
    34321 ctggatgcat tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat
    34381 tacacacata cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg
    34441 atttgcgcag gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct
    34501 ggcttcggat ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta
    34561 gttatccctg catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat
    34621 atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac
    34681 ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta
    34741 gtattggtga tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca
    34801 agccgaaaag aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat
    34861 gatgaaatga ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt
    34921 gcggattagt ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa
    34981 aaagacgaga ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc
    35041 aaatcgtctt taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca
    35101 gtgaatacgt gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc
    35161 ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag
    35221 caaaaacccc gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga
    35281 aatatcgtta tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg
    35341 cgacaacaaa agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac
    35401 cttacctagg gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc
    35461 atgctggaca tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga
    35521 atgctggatt cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg
    35581 gagaccgtca gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag
    35641 aaatccaaac ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg
    35701 aagtcacatt gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg
    35761 acaaaccgga tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag
    35821 tagaattcaa agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg
    35881 ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag
    35941 gacaattcgg agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc
    36001 caattgctaa attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac
    36061 caggtgactt gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg
    36121 ctatccggac acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct
    36181 tatgggtaac tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg
    36241 ctcaccatcc attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg
    36301 caaaagtcta tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt
    36361 ctatccgtat tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa
    36421 aagaacaaat ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc
    36481 cacatggcgg tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata
    36541 atattcgtga agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg
    36601 caccaagtat ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag
    36661 aagaatagcc gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg
    36721 atttaattga aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga
    36781 aaaaaacatt cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg
    36841 tttatgcgtt gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt
    36901 caagtggtat tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat
    36961 tcgaagtacc ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt
    37021 ggttaaaaat cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc
    37081 tttgtatcca tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt
    37141 ttgggggggc tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg
    37201 gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta
    37261 tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat
    37321 atatgtttta cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata
    37381 ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg
    37441 aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata
    37501 agcatgataa gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta
    37561 gagaagcaat gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga
    37621 tacttggtcg tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata
    37681 ctgctgtaga cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa
    37741 gaaaaactgc ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt
    37801 taaatttatg ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt
    37861 tcacttaata cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg
    37921 gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca
    37981 ttagctgtgg tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat
    38041 ttgggcgttc cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag
    38101 gaatgggaac aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga
    38161 ctctatccgg cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag
    38221 cgagggctgt taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac
    38281 tggtggcaaa tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt
    38341 acgcatatct ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt
    38401 gtattgttga tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca
    38461 tccttcctct ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt
    38521 gtttattacg gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt
    38581 gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat
    38641 atcgctggag gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat
    38701 tacaatttca tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt
    38761 gcagtgacag cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact
    38821 tggattggca aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat
    38881 gaagcaaagg tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca
    38941 ttgattctaa tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt
    39001 gattatggtc gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag
    39061 agcaaaccat gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt
    39121 acggcaccga aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa
    39181 agtaaaaaat tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac
    39241 caagcgatcc ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta
    39301 gaactaacgg catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt
    39361 tatccaaaag tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac
    39421 attgaaaaat taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg
    39481 aatggctcat tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa
    39541 gtatactggg tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg
    39601 cttgaacaga tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt
    39661 aatgaccata gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca
    39721 cattacattc acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt
    39781 gagacaaacg tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc
    39841 ctgaagtttt tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg
    39901 gttgagtgtg tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct
    39961 tctgcttttg ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt
    40021 cttctttttt gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt
    40081 ctagaaagat cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa
    40141 aaataatgtt catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat
    40201 attgtaagtg atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc
    40261 ttcaactaac aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt
    40321 aggtgcaatt cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg
    40381 gaccatagta aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc
    40441 taacgtcgta tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt
    40501 tgttggcagc cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg
    40561 aacgacagat aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg
    40621 gttaagcgcg atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc
    40681 gatagccgcc tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag
    40741 gtcaacaatc ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa
    40801 agaaaaagaa aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc
    40861 gattcgttcg tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta
    40921 ttatactatt tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat
    40981 actatgacca taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag
    41041 ttcgagtact attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac
    41101 cgcaagatga gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc
    41161 tggcggtagc tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa
    41221 atccagttct gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc
    41281 caggaccaag cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga
    41341 tcatgtttcc cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt
    41401 ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg
    41461 atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata
    41521 tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg
    41581 acaaattgga caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg
    41641 ataagtttat gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca
    41701 atgaaaacga gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag
    41761 aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg
    41821 tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta
    41881 tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga
    41941 aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga
    42001 tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat
    42061 ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt
    42121 cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt
    42181 taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt
    42241 tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca
    42301 ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag
    42361 tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga
    42421 actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag
    42481 acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca
    42541 aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat
    42601 cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc
    42661 cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat
    42721 catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg
    42781 ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc
    42841 tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca
    42901 agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa
    42961 accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc
    43021 acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac
    43081 gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg
    43141 aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt
    43201 tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg
    43261 aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa
    43321 aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact
    43381 gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg
    43441 taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac
    43501 tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa
    43561 attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga
    43621 agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat
    43681 actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa
    43741 gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac
    43801 gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa
    43861 acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca
    43921 agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact
    43981 agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg
    44041 acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat
    44101 tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa
    44161 agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg
    44221 aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg
    44281 acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac
    44341 tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa
    44401 tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa
    44461 gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg
    44521 atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt
    44581 aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat
    44641 tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa
    44701 aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa
    44761 aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca
    44821 atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag
    44881 acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa
    44941 aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga
    45001 tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga
    45061 ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac
    45121 gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga
    45181 aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga
    45241 agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac
    45301 ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca
    45361 gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg
    45421 tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca
    45481 aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct
    45541 tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt
    45601 ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct
    45661 ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg
    45721 agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc
    45781 gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc
    45841 cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg
    45901 atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga
    45961 aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt
    46021 acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg
    46081 ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg
    46141 acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt
    46201 gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa
    46261 agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt
    46321 tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc
    46381 aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg
    46441 tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga
    46501 aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat
    46561 cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca
    46621 agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa
    46681 acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac
    46741 gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct
    46801 gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt
    46861 gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc
    46921 tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt
    46981 tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac
    47041 gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc
    47101 agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct
    47161 gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc
    47221 cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt
    47281 cgtctatggt gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc
    47341 ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta
    47401 tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata
    47461 tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag
    47521 aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga
    47581 gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga
    47641 tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt
    47701 tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga
    47761 atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca
    47821 aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga
    47881 tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag
    47941 agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg
    48001 tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc
    48061 ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta
    48121 caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg
    48181 gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta
    48241 tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct
    48301 tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt
    48361 agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt
    48421 gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga
    48481 acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca
    48541 aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga
    48601 agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat
    48661 ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg
    48721 acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct
    48781 cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt
    48841 ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc
    48901 gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt
    48961 taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa
    49021 tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga
    49081 acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat
    49141 gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact
    49201 gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat
    49261 ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta
    49321 atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga
    49381 ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa
    49441 ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata
    49501 tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag
    49561 ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct
    49621 tctggaagca tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg
    49681 aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt
    49741 tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca
    49801 ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca
    49861 agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag
    49921 tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct
    49981 cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga
    50041 tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc
    50101 tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac
    50161 gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt
    50221 ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa
    50281 ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga
    50341 acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct ctg
//
LOCUS       DAFGFZ010000005        46848 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000005 DAFGFZ010000000
VERSION     DAFGFZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.005"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(152..1126)
                     /locus_tag="LR726_000235"
     CDS             complement(152..1126)
                     /locus_tag="LR726_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL6215351.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1107..2990)
                     /locus_tag="LR726_000236"
     CDS             complement(1107..2990)
                     /locus_tag="LR726_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL6215352.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(3008..5233)
                     /locus_tag="LR726_000237"
     CDS             complement(3008..5233)
                     /locus_tag="LR726_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL6215353.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5348..6382
                     /locus_tag="LR726_000238"
     CDS             5348..6382
                     /locus_tag="LR726_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6215354.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6449..7462)
                     /locus_tag="LR726_000239"
     CDS             complement(6449..7462)
                     /locus_tag="LR726_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6215355.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7540..7728)
                     /locus_tag="LR726_000240"
     CDS             complement(7540..7728)
                     /locus_tag="LR726_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL6215356.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8168..8296)
                     /locus_tag="LR726_000241"
     CDS             complement(8168..8296)
                     /locus_tag="LR726_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL6215357.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8299..9672)
                     /gene="rlmD"
                     /locus_tag="LR726_000242"
     CDS             complement(8299..9672)
                     /gene="rlmD"
                     /locus_tag="LR726_000242"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL6215358.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9783..10838)
                     /locus_tag="LR726_000243"
     CDS             complement(9783..10838)
                     /locus_tag="LR726_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL6215359.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10848..12278)
                     /gene="gatB"
                     /locus_tag="LR726_000244"
     CDS             complement(10848..12278)
                     /gene="gatB"
                     /locus_tag="LR726_000244"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL6215360.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12275..13747)
                     /gene="gatA"
                     /locus_tag="LR726_000245"
     CDS             complement(12275..13747)
                     /gene="gatA"
                     /locus_tag="LR726_000245"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL6215361.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13747..14052)
                     /gene="gatC"
                     /locus_tag="LR726_000246"
     CDS             complement(13747..14052)
                     /gene="gatC"
                     /locus_tag="LR726_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL6215362.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14158..16194)
                     /gene="ligA"
                     /locus_tag="LR726_000247"
     CDS             complement(14158..16194)
                     /gene="ligA"
                     /locus_tag="LR726_000247"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL6215363.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16211..18445)
                     /gene="pcrA"
                     /locus_tag="LR726_000248"
     CDS             complement(16211..18445)
                     /gene="pcrA"
                     /locus_tag="LR726_000248"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL6215364.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18600..20159
                     /locus_tag="LR726_000249"
     CDS             18600..20159
                     /locus_tag="LR726_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL6215365.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20270..22201)
                     /locus_tag="LR726_000250"
     CDS             complement(20270..22201)
                     /locus_tag="LR726_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6215366.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(22219..23133)
                     /gene="pfkB"
                     /locus_tag="LR726_000251"
     CDS             complement(22219..23133)
                     /gene="pfkB"
                     /locus_tag="LR726_000251"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL6215367.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23130..23882)
                     /locus_tag="LR726_000252"
     CDS             complement(23130..23882)
                     /locus_tag="LR726_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL6215368.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(24041..24250)
                     /locus_tag="LR726_000253"
     CDS             complement(24041..24250)
                     /locus_tag="LR726_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215369.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24261..25589)
                     /locus_tag="LR726_000254"
     CDS             complement(24261..25589)
                     /locus_tag="LR726_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215370.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            25725..25880
                     /locus_tag="LR726_000255"
     CDS             25725..25880
                     /locus_tag="LR726_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215371.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(25954..26541)
                     /locus_tag="LR726_000256"
     CDS             complement(25954..26541)
                     /locus_tag="LR726_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL6215372.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26624..27874)
                     /gene="clpX"
                     /locus_tag="LR726_000257"
     CDS             complement(26624..27874)
                     /gene="clpX"
                     /locus_tag="LR726_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL6215373.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28121..29410)
                     /locus_tag="LR726_000258"
     CDS             complement(28121..29410)
                     /locus_tag="LR726_000258"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL6215374.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(29564..30496)
                     /locus_tag="LR726_000259"
     CDS             complement(29564..30496)
                     /locus_tag="LR726_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215375.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(30653..31312)
                     /locus_tag="LR726_000260"
     CDS             complement(30653..31312)
                     /locus_tag="LR726_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL6215376.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(31315..32340)
                     /gene="sppA"
                     /locus_tag="LR726_000261"
     CDS             complement(31315..32340)
                     /gene="sppA"
                     /locus_tag="LR726_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL6215377.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(32353..32643)
                     /locus_tag="LR726_000262"
     CDS             complement(32353..32643)
                     /locus_tag="LR726_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215378.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33052..34857
                     /gene="glmS"
                     /locus_tag="LR726_000263"
     CDS             33052..34857
                     /gene="glmS"
                     /locus_tag="LR726_000263"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL6215379.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(34929..36284)
                     /locus_tag="LR726_000264"
     CDS             complement(34929..36284)
                     /locus_tag="LR726_000264"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL6215380.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(36308..37477)
                     /locus_tag="LR726_000265"
     CDS             complement(36308..37477)
                     /locus_tag="LR726_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215381.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(37474..38361)
                     /locus_tag="LR726_000266"
     CDS             complement(37474..38361)
                     /locus_tag="LR726_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL6215382.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(38550..42242)
                     /gene="nifJ"
                     /locus_tag="LR726_000267"
     CDS             complement(38550..42242)
                     /gene="nifJ"
                     /locus_tag="LR726_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL6215383.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            42441..42962
                     /locus_tag="LR726_000268"
     CDS             42441..42962
                     /locus_tag="LR726_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL6215384.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(43661..44218)
                     /locus_tag="LR726_000269"
     CDS             complement(43661..44218)
                     /locus_tag="LR726_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215385.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(44397..45008)
                     /locus_tag="LR726_000270"
     CDS             complement(44397..45008)
                     /locus_tag="LR726_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215386.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(45121..45378)
                     /locus_tag="LR726_000271"
     CDS             complement(45121..45378)
                     /locus_tag="LR726_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215387.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(45975..46253)
                     /locus_tag="LR726_000272"
     CDS             complement(45975..46253)
                     /locus_tag="LR726_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215388.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(46287..46538)
                     /locus_tag="LR726_000273"
     CDS             complement(46287..46538)
                     /locus_tag="LR726_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215389.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        12864 a        10078 c         8288 g        15618 t
ORIGIN      
        1 tgtttttcag aaaaataaca attggtttta ttgaagaaaa tagtcatgaa caaaagtatc
       61 tgagaacttt tttactctct tgttcatgac atatagaata aagcagcttt gtcttgtaaa
      121 ctccgttttt caaataataa tctaaagatt gctactgaat aataaagccg atttcatggt
      181 cctcttcgct gctgattcgg taaggtgact caacatccgc tccccagcta ttgatacccc
      241 cgactcctct tacttttgca aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt
      301 ctatatgtgt tgcctgttcc agttcttctg ctttgaatgg taaagcggaa aaagcaaatg
      361 gcgtatcctg ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc gtattatcca
      421 acgtttgatt tcttgttagt tctacccatt tcgtttccat atgcatgccg cattcttggg
      481 gaacaaggta tggggaaacg gggagtcctg ccactttata aataccttcg acaccaccag
      541 ccatccgatc cggatatgtt tcccctgata ggccttcata ctcaaaaaat tttgctttcg
      601 ttggcataat gaaccttaaa cccaatgcgg gtaattccgg taatcctttt tgtccataat
      661 aatgcatatt gacaagcatt ttaccctctc cagcgatttg gtaggaaatt gtcacttggg
      721 tttttggtac tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca gcgtattcat
      781 tacttgaata ttgattattt actggagcaa tcggcaaatc gatcttttta ttgtccacca
      841 gtacttctat ttctttgact tttggataca atccggctcc taaccacata ccggatttaa
      901 atccaaatcc gctacctctg tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt
      961 cccggtatag ccattctttt ccgtttttat tgagcgattc cagtccgcct cttgtataag
     1021 aaaaaatata atgcgtattc cccgatttga cacctaatgt acagtcgccg aagacgattg
     1081 caactttttc cgtatccata tgacttttat ttactgtatt tttcataata atatcttacc
     1141 tcctgcatgg cgggtttttc ttgtccatct gcaaaaacga tcccattccc tgagaattca
     1201 tagtcacttg gtttatcgtc aaagtcaccg ccatagcgca tcacttctct gcctgtgatt
     1261 ggatcagtca cttggatcgc ctgatcttta aaatcccata tatagcctcc ttggtacatt
     1321 tcataacgat caagcaacgt catgtaagaa tccattccac ctaacgaatt ccccatgtca
     1381 tgcatatatt cacataagat gaacggtttt tccgctttat tttcaaggta gtcaatgatc
     1441 tcttctggtg gcgcatacat gcagcttttt acatctgaaa tcgaatcttc gaatgctgga
     1501 tttggaaaat tcccttcata atggacaagt cgatcggggt ctactttttt gaagtaatcg
     1561 ttcatttgtc gaatgttttc ttccgcataa gattcatttc caagagacca aaaaagaata
     1621 cttggatggt tttttaatgt ttcgaaattc gtttttgctc gatcaagaac agccgcctgc
     1681 cactgaggta ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat tttttgccat
     1741 gagccatggg attcgaggtt cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa
     1801 taataccaag tgatctggtt ggggtaatga gatgtccgta cgccgttgat ctttgcattt
     1861 tttatgatag ccaaatcttt ttccatatct tctatagtga tggcgcgtcc ttttttcgga
     1921 tgccactcat gtcgatttac cccgttgata atcatccgtt ttccattgaa gtagatgatt
     1981 ttatctttga tttcgatttt acgaaaaccg aaggaatagt caatatactc cgttactctt
     2041 ttttcttcgt ctatcgtctc caattcaagc ttatataaat tgggctgaaa atgcccccac
     2101 agtgccacat tttcaaatcc atcgatgcga atcgtcattt gctcttttaa cggatattgc
     2161 tgttgataga tttccttccc atcaggatct agtatcgcta cattcagctg actgtttttg
     2221 gctacttgct caaaagacaa atctacttca agtttgccat tttgacattt gtcatctaag
     2281 cgaggtttga tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa gaagacatct
     2341 ctgaagatcc caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt
     2401 ttgtaaacct ccacagctag aatattttct tcttccaaat aaggtgttaa atcaaattca
     2461 gacggagtga acgaatcttc ggcatacccg ataaaatgac cattcaacca aataaacatt
     2521 gcttgttcta caccagcaaa atgaatcgag atttgttttc cctttagacc ttcttctaat
     2581 cgaaaagtcg tcaaatacga accaaccgga ttgtctttag cagctgaaaa gatcccttcc
     2641 gcctctttct cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga
     2701 tacatcgtat tgatgtaatg gatcttgtca taatttgcta attcaatatg actaggaaca
     2761 gtgatttcat caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc
     2821 gaaggatttg gcgaataaca aaaacgccat gatccattta aagactgttt aagggaggat
     2881 gtggtttcta cttcttccct tgttgcataa caaacatgat cgctatgtgc atttgattgg
     2941 ttcactctaa aaacttgtgg atcatctaac cattttatat ttgctttcat acttttttcc
     3001 ttctctctca taactttttg aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg
     3061 ggaagtaaag tccaactgtc atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt
     3121 cctgataaag tgcctcatca tctaacccca tcagatatac ttcatggaac gctggttgag
     3181 ctgaagctag tatcctccct aaaaacacaa tagcttcatc ttgtttagga gacacaaaca
     3241 tccatgcggc aatattttct tcaaaaggac tcgccaaacg ataataatgg ccatactgga
     3301 tcaccggacg aatcgtttga taggatataa tttggtcttt tacttgcttt tcttcttcag
     3361 gagacagctt tgtcaaatcc agttcataac caaaaacagc gctcatagct gtttctcctc
     3421 ttgttttcaa cgaagtagat ctgtcggtct gatgatttgg tacagctgat acatgagctg
     3481 tgatggacga aatcggataa gcaaggcttg ttccatattg gattttcata cgctccactg
     3541 catccgtgtt atcacttgtc catgactgcg gcatataata aataaacccg gcatcaaacc
     3601 gtccacctcc accagaacag ccttcccata agatttccgg ataatcagtt gtcaaatgtt
     3661 caagtagttc ataaagcccg agcatataac gatgcgctac ttctccttgt ctctgtgggt
     3721 caagggtaat cgaatagaca tctgaaagcg aacggttcat atcccatttg atgtagtcaa
     3781 gaggaatcgt atctaaaatc tttctcattt gtttctcaat cgtctgccga acatctaacc
     3841 ttgtaaaatc cagtacatgt tgtgatctgg aagcagaagg cattctacct ggctcctgca
     3901 taagaaaatc aggatgtgtt cgatacagtt cagaatcaac cgagatcatt tcaggttcaa
     3961 accataaacc aaatttcaat cctttgctat gagcataatc tgctatttca cgtaatccat
     4021 ttgtcaactt gccttcatac tcgaaccagt ctcctaatga agaattgtcc gcatctcttt
     4081 ttccaaacca gccatcatct aatacgaaca tttcgatgcc tagctccttt gcttcatcaa
     4141 tgatcgcctt gattttttca ctattgaagt caaagtaagt ggcttcccag ttattgacca
     4201 gaatcggccg ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt agcaactcat
     4261 ggtggatctg actcattttg ttcaatccct gacttgagta agaaaggatc atttcaggtg
     4321 tttgaaaaga ctggcctgca ttcaattccc acgaaaaacg atggctgttg atcccaccga
     4381 cgactctcaa ttgattgatc tgatcttttt caagttcgaa agaatggttg ccggaataaa
     4441 caaattgaag tccgatcgct tctccagtat tttctgtcgt atgatgatga actaaagcaa
     4501 taaagttatt catatggtga ctgctcgttc ctcgacggct ttcaaatact tttgttccag
     4561 ataaaacaga ttgccgtgaa atctgtcttt cattgacatg tgctcctggc aaagcaatga
     4621 cttcatcaaa tcgcctagta tgggcaaagt ctagctggaa actagccgct ttttctagtt
     4681 tgattgtttc tccggtttca tttcttatct ttacagatcg tgtgatcact gctctatcag
     4741 taaatatggt atagaacaaa tcgaaataaa tcgctgtttc tctatcctta agtgaaatca
     4801 ccaacgtttg tgcctctgac ttgtccatca cataagtttg aggcaagcct tcgatctctg
     4861 gcttcccatc tgtaatataa tggctgtcat atctcagatc cagtaagtct gatccattct
     4921 tgcgctgaat gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa gagtattctt
     4981 gcggtagtgt atctttcgac agagaccggt caggatttaa gggaacattt cctgaaaatc
     5041 cacgatctct tcggggataa tttttattgt ctttgtactg cttgactggt ttgccaaaat
     5101 aaatatgcgc caatacattg ctttcttcta tctttatcag ataagaaaga aatgtatttc
     5161 gtaaatggaa gactcggctc ttttcatcaa ataagatgaa atttttatct gtttttctat
     5221 gcgtaatatc catcatttca ctcccaacaa aacggaaacc gtttactttt ttttataatt
     5281 cctgtatact gtttactaaa caattttgtc aataaaaatt ttttattttt ttgaggagtg
     5341 tgtaaaaatg gttggaatta gagatgtcgc acgaaaggca aatgtctctc ctgctacagt
     5401 ttcccgtgtg ttgaataacg atcagacgat ccatgtggct cagactactc ggcaacggat
     5461 agtagaggca gctgctgcgt tggattatga catcagtact cgtaaatata tcaaaaagag
     5521 aatgccttcg attggtgtga tctctacgat cggcaaagcg agcgaagaac aggatatcta
     5581 ttacaaacat ttgcgagtag ggatggaaga agaagctcga cgtctgcatt tagggatgaa
     5641 tcgaatctat aatctatctg ataatcctaa acaatgggaa gatctagatc agttaggtgc
     5701 ggtgatcgtt gttgggactg ttacgaaaga atcgattacc gccttgttaa aacaaaacat
     5761 gcaaatagtc gtcgtggata accctgacat ccagcaagag gtcgatatgg tgtatgcaga
     5821 tcttgaacgg atgactaaaa ccgtactgga attgttctta gaaaatggtc ataaaagaat
     5881 agcctatatc gggggatata aagtcgatat ggatgaattc ggtgggaagc agatttctat
     5941 aaatgaaaag aggatgcgtg cctatcagca ttttatgatc gatcatcact tagaaaaata
     6001 tatgtcttat catctaggtg gctggacaga aaaacaaggt gaggtacttg cgactaatct
     6061 attagaaaat gaatcccctc ttcctactgc cattcttgtc gggagcgatc caatggcaat
     6121 tggtgtctat cgggcattga aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc
     6181 aagctttgat aatatcgaga caatcgcaaa cctctcccct ggcttgacca gtgttcaaat
     6241 caatgcgaaa tcaattgggc aagcagctgt tcgaatggca ttggagcgaa ttgacggtat
     6301 acgggacgaa ccaatcattt tgacttatcc tgcacgattg attgttaggg aaagttttat
     6361 cccaagaaca aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact
     6421 caaaacgaaa cggctttttc acaacctctt acttatactt attcacaaaa tccaatgtct
     6481 gctggtaaca attttgcgat tcttttagcg tgtaatcaaa ttggtattca tgggtaatct
     6541 ctttttttgt atctgtataa aacaaggatt gcacgggtac ctttaattct gtcaaccgat
     6601 tttctaatgc ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt atatacgttg
     6661 gcggaaattc cttagaaaca tggtcagcca cactagcttc tttcaattgc tcactcgttt
     6721 tccaatcttt tgttccaatc aacgaccacg caacggtttt gacgaaaaac ttcataaatc
     6781 gatcatccga atgctgctgt gctgttagct tcaaatcaac aggtccgcaa taagaaatgg
     6841 ttcctttcag actatctttt ggaagtagct gtttgatttt catttgttta gcatacgcat
     6901 cattcgtttg tatagctgca tattgcaatg cgatttgtga acctgcacta tctccaccaa
     6961 aaaataattg ttccatgtct agcaatttgt cgtctttttc ttcagctaat aaatctttga
     7021 tcagttcatt cacttgaaga acttgattag ggtattctga atcaggcgct aattcataat
     7081 tcatcgcaac cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat tctttgacac
     7141 ccgatttatc tccgccgacg aatcctccac catgtaccca aaatagaaca ggaacaggcc
     7201 ccttgctgtt ttttggatag tagatgtcat aagtattctc tttatacttg gactcatacg
     7261 tcaaatctgt ttttttcgcc acgttttttt ctgcttgatc gaacgttttt ttatcagtga
     7321 tttgaacagt atttgaaaac atatggttga tcaagaatgc tccaggtctg ggggaaagct
     7381 ggaaggcaag aaagattgca aacgcgatcc caagaatgat tccgaagaac catccgataa
     7441 atttaagaat tttctttttc ataaggctcc ctcagtagtt agtattaagc attctgatta
     7501 taccatagtg ttatttccta tcaagctctc ctagatgatt taatgatttt ccccaaactt
     7561 cttctgcaat gcttttgaca aaagaaattt tttccattgt tcttcaaaca aaacattttc
     7621 ttcttctgtt gaagcaaatc cacattgagg acttaagcac aaacgatcca aaggcagata
     7681 tcgtgtcgct tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt cgccattttt
     7741 cgttacagtc aaaatctaag acaactaatt tatcttcaga gactttctct aaaagaacaa
     7801 aactcccagc acgttcacta tcgtattcta aataataggc acggacattt tcttgagtaa
     7861 aataatgggt ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa taataatttc
     7921 cgcgtcaaac atgtatattt agtgttgaat catttgagaa tatgaaaata aaaatctttt
     7981 tgatcaatca gttaatcaaa ttgaaataac gaaaaatctg agacagaatg acgttcattc
     8041 tgacccagat ttgattttgc acaactcaac agtccttatt ggcaaatcgc caaaataatg
     8101 tagactaact aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt
     8161 agcacgtcta tttattaaag attttagggc cttttctcat gtttttacga tcattcttag
     8221 gtgtttccat ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt
     8281 tttctttttg gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg
     8341 ggtttgcggg aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa
     8401 taacttagca tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg
     8461 tgacatttcg attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac
     8521 tacgatcaca gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt
     8581 tccgacttca taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc
     8641 aattgcttct ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa
     8701 gccaatcgta cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc
     8761 aaactcgatg gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga
     8821 ctttgctgag attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata
     8881 taaaactttt tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat
     8941 gaccgaaacc acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa
     9001 gaatttttct ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg
     9061 gataatgatg tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa
     9121 ccgttgcaag atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata
     9181 aaaatcttcg atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg
     9241 tccatcgata cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat
     9301 tccgaccgtt ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc
     9361 tacttgtttt tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat
     9421 acctatgcga agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa
     9481 tgtttcgact tttccatatc caaacttatt gcctgctttt actactagta tttctacttc
     9541 ttctccaggg agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat
     9601 tccttcataa ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg
     9661 ctgttctgtc atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata
     9721 aatcagctaa ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt
     9781 gattaattct tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca
     9841 tccgttaaac gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca
     9901 tctgtacctg tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt
     9961 tgatgaagat tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc
    10021 attactggtc gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag
    10081 attattcttg gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag
    10141 atctcaaaga tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg
    10201 gcatctggcg cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc
    10261 gaagcatttc cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact
    10321 cgagggagca tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt
    10381 tctgatggaa cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag
    10441 taactgttat cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct
    10501 gctgctgctt taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta
    10561 ccagctggga tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact
    10621 tcgttgattg tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact
    10681 ctacatgctt cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca
    10741 aatcctgctt cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt
    10801 cccgaggtgg ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct
    10861 aattcatctt taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc
    10921 atgatttgac cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca
    10981 gattgttggt tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat
    11041 aattgcaccc aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat
    11101 tcacggaaga cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc
    11161 attcctgcta agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc
    11221 aagtaagcag aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt
    11281 tccaaaacag cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca
    11341 tactctggaa gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc
    11401 aaactagctt ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc
    11461 tctgggaagt aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc
    11521 gttgtttcgt caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt
    11581 gcttgacgtt tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt
    11641 ttcaattctg ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca
    11701 tcacagcgca tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt
    11761 gatcgcaatg cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa
    11821 acgatttcga tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata
    11881 ccatgcatgt tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc
    11941 tttttacctt ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg
    12001 gaaatttgat atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta
    12061 tctttagaga tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct
    12121 ttattcatta ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt
    12181 ggttctgcac caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg
    12241 acgtggactt ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag
    12301 ctggttgttg tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg
    12361 ttttttcatc aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac
    12421 ctgccggtac agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac
    12481 tcatgtacat tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg
    12541 aagatggccc gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca
    12601 atgtacggac ttgtcctgct tttttgaagt aagcgtcata ataacccgcg cttaaggaga
    12661 atgtacctaa catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga
    12721 catatacatc atctaatgat ttcacatttt cagagcgata accgtagcgg ataccatcga
    12781 atcgttgcaa attggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt
    12841 attttgaatg aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg
    12901 ctgctttcaa tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag
    12961 gtaaagcaat cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg
    13021 aaacgcctga agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg
    13081 cattatcttt caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa
    13141 tcaaaccaaa acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag
    13201 ctgctggttg gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag
    13261 ctgcaactgc tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag
    13321 cattttttgt tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca
    13381 tgtttagctt accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat
    13441 cataaattgg ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg
    13501 ttacgatatt atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc
    13561 ctttttcatc taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg
    13621 cctcgacctc aggctctgtt tctttgattc gttcaaatgt tgtttttgtc aaatcttgta
    13681 cagtgatttc tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt
    13741 ctgtcattat gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc
    13801 ttctgtttcc ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc
    13861 ttgtcgcatc acgtttactg tttcaaccac attcgacgta aatggaacac cttccgtatc
    13921 tacttcttcc agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc
    13981 aagttcttca tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc
    14041 actaatcgcc atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa
    14101 cacaagagtg ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca
    14161 ttcttctact gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc
    14221 gattcctaat tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat
    14281 atccgttttt ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt
    14341 tgcttcttca cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa
    14401 aggagactcg acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc
    14461 tgcttgtttt aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac
    14521 actgtcggca atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt
    14581 actcaatgtt tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac
    14641 atgtcggatt cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat
    14701 tgctgtcaaa atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc
    14761 ttctgtcaat ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc
    14821 tagtacgcgc ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa
    14881 accttctttc atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc
    14941 caaatggaca agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct
    15001 gtctttaggt cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt
    15061 ataaatcaga actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg
    15121 caggctggca cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg
    15181 cgttactacc cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc
    15241 ttctggcgga aacttgtaag ctgctgccca tctcggtgct ttgatagtaa aaccaagttg
    15301 atcttgtagc gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc
    15361 cacacgtttg tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag
    15421 acgtttttcc cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg
    15481 ggtttttgct tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct
    15541 tttcgctgtg attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt
    15601 cgcaaagata tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt
    15661 tggcatgaaa cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac
    15721 cgaccggaca gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt
    15781 cgcacctctc acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt
    15841 tagttcacag caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc
    15901 tatcagctct tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac
    15961 tttttcaaaa ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat
    16021 caaatcagga tattgttcct cgattgctac taattctgcg tattctttat cataaacata
    16081 gtcttccact gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt
    16141 acgaagttct tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta
    16201 ccctccacta ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt
    16261 gacttgggaa agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg
    16321 ttcctagacc ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc
    16381 ctccagtagc acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg
    16441 tttttttagc gaatacaggc gtttttttag gcgtaggacg catgccttcg atttccagta
    16501 actcctgatc gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata
    16561 atgttctgga gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat
    16621 atgcgagacg gcgttcttcc tccagttcgc tttcttccat caatgctcga gataaaggaa
    16681 aaaccccctc ttctagacca atcaggaata caactggaaa ttctaatcct ttggctgcat
    16741 gcaaagtcat caatgtcact tgggaagcat cttcttctaa attgtcaatg tcggaaacaa
    16801 gtgccagatc atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat
    16861 tttgctgttc gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt
    16921 cgatgcgtgc ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac
    16981 gatccaaaac ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt
    17041 cttggatcat ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca
    17101 gatccacatt ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta
    17161 gtttttcgat agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac
    17221 tgattgaatc ttgaggattt accagcacat tcaaatacgc taaaatatct ttgatttctt
    17281 tacgatcgta gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag
    17341 tttcttccat tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat
    17401 tccgatgatt gcttcgaatc tcttcttgca tccggtctac gataaaacgg gtttcgtctc
    17461 gttcattatc tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt
    17521 tttttggttt tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt
    17581 tcgtggagcg atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa
    17641 aatccaagat gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc
    17701 ccacgacgca caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt
    17761 gatttgtatc ttggtactca tctacatgaa tataatggaa tttgttttga taatacgtga
    17821 gtgaatcagg atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca
    17881 tacattgatt atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt
    17941 cgtaataact gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt
    18001 gagagattgt tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt
    18061 ttagtatccg tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt
    18121 tatacccgat aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg
    18181 atacccagac atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt
    18241 ttgcagcctt attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct
    18301 caatcaaata ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca
    18361 ttagcagtaa tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt
    18421 ttaataattc agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt
    18481 atagcatttt cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg
    18541 gatttttcct tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa
    18601 tggaaaaaaa aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag
    18661 gaatatttgt tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa
    18721 agctaagcga tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa
    18781 ttggcctagc tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg
    18841 aaccggatat caagggaagc gggattccac aagtagtggg acagcttcgc ggggaattat
    18901 caatgaactg gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg
    18961 cgggcctttt tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag
    19021 gagctggaca attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg
    19081 caagtagcgg acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag
    19141 aagaaatcca tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca
    19201 ctgctaactt cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc
    19261 ttccttcttt gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca
    19321 tcttaggttt tatttatcag aatgtcttgc tggctttgcc aaaattttac aaccgattgc
    19381 ctttaccagc ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt
    19441 tatggcctag tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct
    19501 tgccaatcat tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt
    19561 acggtggggg tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg
    19621 gtagctttta tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt
    19681 ttgtcgttat tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa
    19741 ttattttagt gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg
    19801 ctcttgttgc ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat
    19861 tggaacgtct acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg
    19921 attttccagt aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc
    19981 ctaaaaatat gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg
    20041 atacgatcat gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa
    20101 gaatcaagca ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac
    20161 gaacatggaa aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt
    20221 ctataaaaaa agaagaggct gggaaaaccc agcctcttct tttttatttc tatgcttctg
    20281 ctactgcagg agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag
    20341 caataaacac caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccgt
    20401 gaggagccag taatttgatt ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa
    20461 taataaaact tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg
    20521 tgacaaacga tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga
    20581 atttattctt aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag
    20641 ccataacaga agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg
    20701 taccaaacac ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag
    20761 ctaataatgc accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca
    20821 atgcatcatt gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca
    20881 gtaagccaaa cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt
    20941 tcttgaataa tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg
    21001 cacctaagaa tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca
    21061 aaccagggcg gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac
    21121 caaatgcagc ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat
    21181 ttgataattg atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag
    21241 cgattcctcc accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt
    21301 aaattctttg tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat
    21361 caccatggaa tactggtgca gtaccactaa gggctagatt gatcaactct tctggtttac
    21421 ggataccatc actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta
    21481 cttttttgtc tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc
    21541 ggtttttgat tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg
    21601 cttttttctt caaagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg
    21661 taacggcaac aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt
    21721 gttctgcttt ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt
    21781 cttctggtgt atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag
    21841 atagtgctgc taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga
    21901 aaaacagata agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg
    21961 cgaataaaac tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat
    22021 caccaagacc tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga
    22081 tatcggaaat ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt
    22141 tatcagtcgc ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca
    22201 tgattatcct ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc
    22261 aatcaattcg cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc
    22321 ccatttaaag gcttcgattg gatctttcgt acggctgtaa gccccaataa agccagcaat
    22381 cattgaatcc cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata
    22441 agttccttgt tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg
    22501 tgccccttca gctagtaatt ttttcccata cggaatgatt tcttctacag attggaaggt
    22561 tgtgttgtaa agctcagcta attcgtgatt gtttggtttg atcaacaacg gtttcttaac
    22621 aagtgcattt tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg
    22681 aatgatatca atcagttctt gataaaatcc ttttcgcagg ctagctggcg cgcttcctga
    22741 caatacgacg atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc
    22801 ttcatctgaa atagatgggc ctaagccatt gatctcagtt tcagtgtctg attttagttt
    22861 gatattgatt cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc
    22921 aagccacttc gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg
    22981 agaatcgatc cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa
    23041 gtcttccttc attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc
    23101 aattgatgga ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt
    23161 tcacggaatt cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc
    23221 aaaggtgcaa cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat
    23281 gtctcttcgg aatttttcat ggctaaacgt ttaacagctg cctcttctgg atccggagtc
    23341 gtgaatcctt gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa
    23401 ttttttagtt gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt
    23461 ccacctaaaa taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa
    23521 tttgtcacta ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag
    23581 ccagcgtcta aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt
    23641 tgctgttttt cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat
    23701 aattgctctt tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct
    23761 tgtaaatcac gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag atcttgtgat
    23821 ttcacgattt tctgctgttc aagcaagtct aggatttttt gtctgcgttc ctctgtaagc
    23881 attttcacga tcacctcaat acaaataata gcatattttt tttctactag caatataaat
    23941 cagtcaaaaa cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaga
    24001 aagactggtt ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc
    24061 ttcttgtttt tttcatataa acagaagcag gttttctttc cttatgaaga agataataat
    24121 aaagtcccat cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag
    24181 gaacaaaaaa gagacagacg gataataatg atgccggaac cattattcga taatagagtt
    24241 gtttatccat tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg
    24301 taatctaaaa attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt
    24361 cgccgctttt tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt
    24421 aacggtaatt ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct
    24481 tgatattttt catctgttac ctctacttct agatattccc taatataata acaataagct
    24541 tcgattccat tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgtgat
    24601 cgattctcgt caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt
    24661 tctttataat ctccttgttg gatatcataa ctttttgtta caaaaaatga agcattcttt
    24721 ttaggaacag gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg
    24781 taaatgtatt caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga
    24841 tcaaacccaa gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag
    24901 ttaaaggggt ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt
    24961 tctacttgtt ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta
    25021 taatacattt cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga
    25081 aattggttgc aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg
    25141 atttcatcct ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata
    25201 aactcgctat aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg
    25261 aagtctcgaa tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc
    25321 aataattgat tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac
    25381 caactttcgt ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac
    25441 ggatccgctt gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa
    25501 atagcccaat cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata
    25561 gtcatcaata atttatcttc gccaatcatt ttctcacctc tttcaaatct ttttataatt
    25621 taattataac caatgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg
    25681 attacatgat agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg
    25741 aacgtacaga taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag
    25801 ttgcaccaaa agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa
    25861 aagacaagaa acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga
    25921 aagagtttga ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc
    25981 tgcccacgct tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt
    26041 gcgatcaaaa tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg
    26101 gatcttatct gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg
    26161 atacatctgg atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc
    26221 acgtagctgg tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc
    26281 agttttggat acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc
    26341 gatcaaataa aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt
    26401 ctttcgatca atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag
    26461 agctatttct ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat
    26521 ttcagcttga tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg
    26581 ctgtcgcaaa agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct
    26641 ctgaatcttt atagattact gttggctcac cagacaattc agctgcttct tttgtgataa
    26701 tcactttttc gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt
    26761 caatgatgga acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg
    26821 ctctcaacgc ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat
    26881 attgtttgac tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca
    26941 atttctctaa agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt
    27001 ttagtaaatc ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt
    27061 tttgtgtacc aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc
    27121 catcaaatgc accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt
    27181 gatgtggatg tttacgtccg ccctgaggtg gcacacttgc aacagttcct tctagaattt
    27241 tcagcaaggc ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc
    27301 ttttacgcgc aattttatcg atctcatcaa tatagatgat ccctttttct gcaagttcca
    27361 cattgtaatc agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat
    27421 aaccagcttc tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg
    27481 ctaatgtttg tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc
    27541 ttttttgcag ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt
    27601 gattgtatac ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat
    27661 tcaatacttc aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt
    27721 cataaaattc ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga
    27781 cccctggacc agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc
    27841 gtacggtctc attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa
    27901 gtatgcaaga aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa
    27961 aagaaaaagt gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac
    28021 aaaaaataag gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt
    28081 ctagaagtag cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg
    28141 ctgtacctgt gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt
    28201 ctgtcaacac gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga
    28261 tttctgcatt gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga
    28321 tcacaagatt tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt
    28381 cttctgtaga accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt
    28441 tgttcaagaa ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt
    28501 caacgatttc tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg
    28561 ctgctttttc ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg
    28621 ttgatacttc gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg
    28681 cttttacttc atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt
    28741 gataatcttc tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta
    28801 attgatcttc aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc
    28861 cttttccgcc atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat
    28921 cgccattttc agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac
    28981 gtttgatacg tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta
    29041 gatctttata ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc
    29101 agtcttgacc tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc
    29161 cagcttcttt gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat
    29221 ataaagcttc ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga
    29281 atccaggtac gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga
    29341 tttcgtcttg cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg
    29401 ttgcagtcat ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca
    29461 tgcatacctt taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa
    29521 tccttgtttt tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca
    29581 tttgctgcaa aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt
    29641 catacatcgt ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt
    29701 cgatgtatgt atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttctttta
    29761 acacggaact aagcagcgca tcttcattcc cgtaacgatc tgaaaataca cataattgct
    29821 ggtaacttgc ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt
    29881 cttcaatcgt caaatagaaa acttgatggc attcgttcaa ttgcctaagt gtctccagta
    29941 attttgccct tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga
    30001 taaactcatg ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt
    30061 ccaacgaagg aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt
    30121 gtccggataa ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat
    30181 aaaaatctgt acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca
    30241 agtcaaaata aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg
    30301 cttgcttcaa tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat
    30361 tagcctcaaa gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat
    30421 attgctggtt tgctagtgct tgttgtgcca ctcgcattaa attctcatat ttttctggaa
    30481 aatcaatcgg cttcacgcag tgtcctcctc ttgctcatat tcagctcatg ttcaatagta
    30541 gcaaaacaaa aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt
    30601 tagatctgac ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt
    30661 tgacttcctg gttgaacgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa
    30721 aatcgcccag atgttgcttt ttctgattaa agactgctgg aagataccct aacatcagca
    30781 aaggaaattt tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa
    30841 gttcttcttc atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttggc
    30901 catgattgaa tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc
    30961 cgtagagact cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa
    31021 gtgttaaacg cgtaatagct ccgatacaca aaagatcgac caagaaggca aacattcgga
    31081 taaaaaaacc tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg
    31141 tgtccgtata ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct
    31201 ctctttcaaa agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct
    31261 gctgttgctt atctttttct tgttccttat tttcatttga aagctctgac atgcttactc
    31321 acctccgtaa taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga
    31381 taagatctga ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa
    31441 ccatgtacta gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag
    31501 ctgttgttcc tttcgtatag cttttaaagc atcttcagga aatcccaatt catcgactaa
    31561 acctacttct tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc
    31621 ttcttcagat ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc
    31681 gatatatgct tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat
    31741 atctttcaac gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc
    31801 tgaataattc aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc
    31861 aaagatttta tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat
    31921 gctgacatac attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc
    31981 cgcactttcg tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt
    32041 tggatcttct tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga
    32101 agcaaataaa cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat
    32161 tttttgcgta ctgtctccct cttccaacac atttggagct agttcttcat ccccatataa
    32221 ccaactgttc aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac
    32281 taacgaaacc acaaataatc ctgcagcaat cagaactgct gcccaacgtc ttttattcat
    32341 tctttctcct ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt
    32401 gtcgaaccgc tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgttaattc
    32461 atcttctacc gtaaactcac gagcttgagc aacttccaag gcatcaggtg cgatttcttg
    32521 gatgctatcc cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc
    32581 ttcgaatttg gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc
    32641 catttttact cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga
    32701 tagataaaaa cccattattt tttgacataa aaaggacatt tgacaaagaa tttgatatat
    32761 accaatttat tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt
    32821 aaatgtttcc agataaaaaa caaaaaggag gatagttata ccttttagga tagcgccagg
    32881 tctgtgtaaa cagatgacga ggagagggtt tatcgaaaga ttcggcggat ggccctaggg
    32941 actgcactct acagaaaatc cacaaaaaat gtctgcaaag ataaaacaaa aggattttcc
    33001 tgcagctgga agcattcata cgaattcatt atagaaagta ggatgttttt tatgtgcgga
    33061 attgttggaa tgattggatt aaaaaatgta acaccaggat tgattgacgg tctagaaaaa
    33121 ttagaatacc gtggttatga ttcagctggt atctttgtct cagatggcac aactgattat
    33181 ctggtcaaag cacaaggcag aattcaaaat ttgaaaaaca aaataaatat tgatactaca
    33241 ggaaatatag gtattggtca tacacgatgg gcaactcacg ggcaaccttc tgaagaaaac
    33301 gcccatccac atacttccca aagtggacgc ttcgtgctcg ttcacaacgg cgtaatcgaa
    33361 aactttgaag aacttaaaat tgcttattta gcaaatgacc actttattgg tgagaccgat
    33421 actgagatta tcgctcatct aattgaaact ttcgcgaagg atactacaac gcaagaggcg
    33481 tttttaaaag ctttgcgggt catcaaaggt tcttacgctt tcgctttgat tgatcggaca
    33541 gcacctgatg ttatttacgt cgctaaaaat aaaagtccac tattaatcgg actcggcgat
    33601 ggattcaatg tgattgccag cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt
    33661 gagattgaag atgaagagat ggttaccgtc acatcagaaa aagttattat tcaaaatttt
    33721 gcaggtgaca tcgtagaacg ttcttcattc gaagcacaag tagatgcttc agatatcgag
    33781 aaaggtacct atccttttta tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt
    33841 cgtatcgctc aaaaatacgt aacagaagac aatcgtgtct ctcttgatca aaagctggtc
    33901 gatacattgt ctgatagcga tcgaatctat atcgttgctt gcggaactag ttaccatgcg
    33961 gggttagcag gtaaacagac tttggaaaaa ttgacacaga ttcctgttga ggttcattta
    34021 gccagtgaat ttggctataa cacacctctt ctttcaaaaa aaccattctt tattttcttg
    34081 agtcaaagtg gcgaaacagc agacagccgc caagtgctgg taaaaatcaa ccgtctcggc
    34141 tacccttcac ttacgattac aaatgttgct ggttctactc tttcgcggga agcatccttt
    34201 acactgctat tgcatgctgg acccgaaatc gcagtagcat caacgaaagc ttatactgca
    34261 caaatagctg tactaacttt attagccaaa gctatcggtg ataaaaaaga atttgaaact
    34321 tctcttgtat tcgatatcac tcatgaattg agtttagttg ctaatgcgat ggaatcggtc
    34381 atcgcccaaa aagattattt agaagagttg gcagaagtat atctcagcga tacaagaaat
    34441 gccttttaca ttggacgtgg tgctgactac aacgtttctc ttgaagctgc tttgaaattg
    34501 aaagaaatca gttatatcca ggcagaaggt tttgccgctg gcgaattgaa acatggaacg
    34561 attgctttga tcgaagaagg aacaccagtg atcggaatca tcagtgaaga agtgactggt
    34621 gcgcatactc gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac actagttatt
    34681 gtctcagagg gattagaaaa ggaaagtgac cagctggtgc taccaactgt ccacccttat
    34741 ctaactactc taaccacagt cgtaccgact caattacttg catattacgc aacgctatta
    34801 cggggattcg acgttgacaa accacgtaat ttagcgaaat cagtgacagt agaataataa
    34861 aagaacaagg accagtgtca gacatctgac actggtcctt gttcttttat tttttatgaa
    34921 aatatctctt aatccagtcc gatttcttct ttgactactg ccgcgatttt atcgacatag
    34981 taattaactt tctcatcagt aggcgcttct gccatcactc gaagtaatgg ttctgtaccg
    35041 gaaggacgaa ctaggattct tccttctgat cccatttcag cttctgcctc ttcaatcact
    35101 tttttgattg ccggaacatc catcgcaccg tttttatcac ttacgcggat attcactaat
    35161 ttttgcggat agattgttac ctctgctgca agctctgata atttcttacc tgtctgtttc
    35221 atgatattca gtaactgaat tcctgaaagc atcccatctc cagttgtgtt aaaatctaag
    35281 aaaatcatgt gcccagactg ctcgccgcca aagttataat cgtttttgcg catttcttct
    35341 acaacgtaac gatcaccaac ttgtgtaacg acatctttca aaccgattcc ttccaccgct
    35401 ttgtggaatc ctaagttact catgacagtc gtaacgatcg tatctttttt caatcgattt
    35461 ttttctgcca agtatttagc acagataaac atgattttat caccgtcgac aatgtttcca
    35521 agttcatcca ccgcaataat acgatcgcca tcgccatcga aagctagtcc agcatctgct
    35581 cctttttcaa cgaccatctc agctaaacgt tctggatggg tagagcctac gccgtcattg
    35641 atatttaacc cgtctggatt tgtgcccatt gtataaaaat ctgtttcaag atcggcaaac
    35701 aaacgattaa cacttgttga agttgctccg tttgctgcat cgacacaaat ggtcaaaccg
    35761 gaaaggtcac cgttgatcgt ttgtacaagg aattgagaat atttcaataa gccttctgga
    35821 aattcttcta cagttcccaa tccttcagct gatggacgag gcaattcatc cacttcagca
    35881 tctaataatg cttcaatttc tagctcttgg tcatctacta gtttaaaacc atctgcaccg
    35941 aaaaatttga ttccattatc ttgtgctgga ttatgagaag cagaaatcat gacccctgcg
    36001 cttgcttttt gcaaacgtgt cagataagca acacctggtg tagagatcac acctagctgg
    36061 aacacttcga tacccaccga taaaagtcct gaaatcagcg cttcttctaa catttgacct
    36121 gagattcttg tatcccgccc tactaacacg cgtgggcgtt ttccttcttc ttcatgctgg
    36181 cttaggacat aacctccgca ccgtcccagc ttaaatgcaa gttctggcgt aagttccttg
    36241 ttggcttcac cgcgtacgcc gtctgttcca aaatatttac ccatagttgt aaaattactc
    36301 ctttaactta attttctgag gaatttgttc tgctcatttg tgttgattcg cttgtttctg
    36361 atgtttcttc tgtacttgtg gtcgagctgc ttgtcccaga ttccgaactg gaagaaatgg
    36421 ttgttgtcga tggttccgtt cctgaatcag cagatgaaat acttctactc gtacttgtat
    36481 tttcttgtgt ccgactgctc gtcgaagcag agctactgct tgacgtttgc aacacgggtg
    36541 taatctccac actaactgat tcgggcttga tacttaaccc ctcttctact ggtatttcaa
    36601 ttttacgagt tgttcgatga ttgatacctg agacattgat cggtacagca atactgtcac
    36661 cgatcgatgc aagcaaatcg ctagagccgc ttacttctgc ttccagtttt gaaaaactga
    36721 aatcatagct ttctaccccg gcagctggtg ttccttgctg tatcccataa agtcgtaccg
    36781 acttgcttgg cttgatcgaa tcagctctca ctgttacttg cactggatca gagacgatac
    36841 ttaatacttc ccctgctgca ttcagcgcct gtattgggac ctcactatta atcccatcat
    36901 ctgtgaccat cgaagcgtcc acatcagcta ctactcgttt gatttctgcc aatgttttgt
    36961 cacctgtagt gatagagact tttttaggac taacggaagt atcatcaagt tcatatccag
    37021 atggtgtgaa cgttgaagga atcaacgttt ccacatcgaa atcttttatt acctttttct
    37081 caaccgttac tgtaatagtc gatggttcga tctttgctgt gacagcactg ctcaaatttt
    37141 gaatttttag cggaacttca tgtgttcctt cgcccagctt agtgatatct gctgtgacgc
    37201 ggaacatccg tgtatcttcg tttgcctctg cattcaactg aactcggttt gcactggaaa
    37261 gtttgacagt cactgcattt tcatatccat ggatatagta gcgctttgaa tcataagaaa
    37321 tcttgatcgg cacccccgta actgtttgat tatacgcttc atttccagaa agcgtatttg
    37381 gaacggactg gccatttgca ttgaagtaca gcgcaatact gaacagcaat gcaataaaac
    37441 tgtaaagaat gcttgtccgt ttctcttttg agatcatttg cgatcccctc ccttagtcat
    37501 accgtctagg aaagattgaa tcacgttttt cttgtttttt gttttctctt cagcaattag
    37561 ttctctacgc aagattgcca aatattcttg ctgggataag ccagccatca gttcattatc
    37621 taacgtgatg ctgacgtccc cggtttcttc agagacaacg atggtaatcg cgtcactgac
    37681 ttcgcttata ccgacagctg cccggtgtcg agtcccgaat tctttaggga tcaacatact
    37741 ttctgataga ggaagatagg cgcttgctac tgcgatcttt ccttctttta caataactgc
    37801 accgtcatgc agcggtgtat taggaataaa gatattgatc agcagctccc cagtgatatc
    37861 tgcatctaaa gcaatccctg tctcaatata ctcatctaat ccagtatgac gttcgatagt
    37921 gatcaacgca ccgatttttc gcttggacat atattggatg gctttgtcaa aagacaagat
    37981 catcttctca tcttcctgct gttcgctttt tgattgtttg aaaagcgaac tgcgtccaag
    38041 atgttccagt cctctccgga tttccggctg aaaaatcacg atagcagcta tcaccccgta
    38101 cgtaatgact tggttcatca gccaagacaa ggtgtgcaga ccaatgattt ccgctacgat
    38161 tcggataaca ataaaaatag ctacaccttt cagaagctga atcgcttttg tgcctcgcag
    38221 cagttggatc agtttgtata caagatacca gacaaccaga atatcaagta tgttcaccaa
    38281 aaaattcaga gagaacaagt caccggaaaa catttgctgc caatagtcca aattaaaaag
    38341 ttccccgatt tgaaatgaca taaaaagcct ctcttctctt ttgagatttc ctctctagta
    38401 tatcatatgt tttaatttta gaggctgata tgctcgtatt tttcttgaga aaaacgcata
    38461 attttcaaga aaattgcaag tatgcgtttt atttttctct gcaataaata tttttaaact
    38521 tcagagaatt attcattata ttttttctgt tatttttcct ttttttcttt tgtagcttgt
    38581 tccatttttt tacgtcgttc tgctctggca gctcttctag cagctcttct ttcttctgct
    38641 tcaaggtccg tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt
    38701 tcaagtcgtt tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt
    38761 gttttagcgt cgtggacagt cttttcaaat aaagcttttg caagatttgg atttgcgtta
    38821 tctagcgagg aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt
    38881 tttttgtagt ccaaagtcat tgggttcttg tttttttcga ccattgtagg gttatatcga
    38941 tataatgacc aataaccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt
    39001 ccgccaagca agccatgtgt gatacaaggc gtataggcaa taatcagaga aggaccagga
    39061 tacttttctg cttcttcaaa tgccttgatc gtttgcattt gattggcacc tgaagcaatt
    39121 tgcgccacat aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca
    39181 tactttccac tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga
    39241 ccgccagtat tggaatagac ttcgttatcc aaaacaagga tattcacgtc tgctccgctg
    39301 gcaagtacat gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc
    39361 atccattggc tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg
    39421 aaaattgatc gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct
    39481 tcactctcaa acatgtgtac taaccagtct tccatcaatt ctcgtagttc ttctgatact
    39541 tttggcaata cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga
    39601 agcataccat aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct
    39661 tgaccacatt cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa
    39721 cagcctgtcg cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca
    39781 taaggtgttt cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat
    39841 tgtgtagaca agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt
    39901 gcaaaagccc aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct
    39961 ttttcttttg cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa
    40021 acttggatac ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct
    40081 ggcgcttctt ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa
    40141 acaaaggcac attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa
    40201 gcgacgcccc gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca
    40261 tttgctacaa gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt
    40321 atttcttgta tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc
    40381 caatggtctg ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg
    40441 tttttttcta cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc
    40501 agcggaagca cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt
    40561 gccgtattga tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta
    40621 tagaatctaa tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct
    40681 aattgttcgt cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg
    40741 accaagtcat agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg
    40801 acaagatacg ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg
    40861 ccagattttt tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca
    40921 ataatcttga tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac
    40981 ttggcctgga atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga
    41041 taagtcacat catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt
    41101 ttcaattcat caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg
    41161 tagcgaccac caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct
    41221 aataagagag gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt
    41281 tttactgtat gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg
    41341 atattcaaaa aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc
    41401 gtctgtgcaa ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa
    41461 gtcaccaagt cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg
    41521 atttcaggta atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct
    41581 ggattctggt tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat
    41641 gaagctaatt tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt
    41701 ttttgaatct cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg gacttctcct
    41761 tcaatagaag caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca
    41821 gctaacatag caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata
    41881 ttcaacgcat ttgtcgtcac tgcccgacta gcaacgtgga aaacacttgg cagtagttcc
    41941 ccagcaatct tgtacatatt cggtatcatc aaaagcagcc cttgtgaagc ggtatatgta
    42001 gttgtcagtg caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat
    42061 tgcatttcta ctacttttac tggttgaccg aatacgtttt cttttcctgc tgctgaccat
    42121 tgatctacta attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg
    42181 gtaaatgcat aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc
    42241 atgattcatc atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca
    42301 tgccattttt ttcttttatt tataaaaata ttttacatgt atctatttga attggtcatc
    42361 taatttttag acaaacagtc taaatcgttg cctttttttg tcagacatgt ataaactata
    42421 agcgaaagag gtgttttaac atggatatat cagtgattga tgcaacgaaa acaaatacac
    42481 aaaaaggaat cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaact
    42541 tagcttgtcc gtattgccga caatggtttg aagaatctta tgagctatta gaagaagcag
    42601 tccaaagcgg gcaattgcag cgagtgatca aattgttcga taaagaaaaa gaaagcttac
    42661 agcgagggaa cgtgatgcac cggtatctca caatcagcga tggccaaaaa gcgatcaaag
    42721 agatcaaaca aatattcgat actcaagacg agtggaaaca tctttctctc caagaagtag
    42781 cggattttgc agtagataaa ctaaagctga cagaacaaaa agacgaacag ctgagccaag
    42841 ctgtcatcaa cgaagctgag caagcacata ttcgatttgt tccaactgtc atcttgggta
    42901 aagaaatctt tgatgaatct atttcaatag aggaattaaa agaattgatc caaacaaaat
    42961 aaaaaaataa ggagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    43021 tataaacggc ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc
    43081 cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga ttatctcatt
    43141 tcttatgaca agctaagcaa attatctcca ccaaataatt tattcatatt tttgacttgc
    43201 gcatactaac taattcttaa attttcatac aaaatttctc aatttccctt aacgagtaat
    43261 atttatgtac ggtgattctc actagtatcc caatcacttt ttaagttatc ccgatttcaa
    43321 ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg gtcatttgtt
    43381 gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc tcgtctacca
    43441 aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg attcgtataa
    43501 agctaacaac tattaactcc tctcagctaa tagttgctct cttttttatt cagattcatt
    43561 tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat tgttccatta
    43621 caacattcac tggctgatga aatggattag tagatttcga ttattcaaag cgattttcta
    43681 ttggtggatc gtgttcccaa ctaataagac ggattccaaa cctatcgtac agatcttcta
    43741 aaccacttat aaattttgca cctatttcag gtgttttatt gcgtggatat tctattgtta
    43801 cgccattaaa tggataccaa ttaggattag gctctctttt tttctgtaca tctcccatag
    43861 aatagttagc actttctggg acataatata ttcttcgacc atcctctaat tcatctaata
    43921 agatatttcg tgatcgtcta tcacttgcta aatgtagacg ccacgtgcga taccatgcca
    43981 tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct tctatcacaa
    44041 tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg gtaatttctg
    44101 gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt cccagatgaa
    44161 acacagctat aacaacaata atgattaata ttttgtttct atttgataaa tttctcatta
    44221 ttcgcacatc acctctacat tctcacatat tagttcgtaa tactccttta atatcaaaat
    44281 atctactggt cgtaataata ttcgctatga attcctacta ctcgtataag aaaggccaaa
    44341 tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat tgttttctaa
    44401 tcaccaaaat tgttaacaat cggttcagat atttcccagt tgattaaggt aattccgtat
    44461 tgtgtcaaaa cttcttgcgc ttctccgaaa ttaagtacag ttcttatgta tccactttct
    44521 cctaatataa agtctacctc tcttttaaaa ccttcctcga gatttctaag ggctatatgt
    44581 ggttttaatg acattgattg tacattttcc cccatataat attgcgcact tcctcgacta
    44641 aaatagattc ctagatcgtc cccaaaaggt agatcttctc taccactagc taatctactt
    44701 ctccatcttc gttctctttt catagaacct gcgctgaact cacacccgtt aaactcagct
    44761 atgacagtat cttcaattat gttaatctgt ccatctagtt cgtattcaat cctaaatgga
    44821 aattctccat aggtgatttc cggttcgggt attttaggtg agattaatgc aaagaaagga
    44881 aatattaagc ttggcaacat caaaaaaact cctcctatga tcacaattcc cgatgtaatc
    44941 caaaatataa tttgtacaat tctcattgat ccattgtatt ccattttatt ccccctatta
    45001 ctattcatat ccgcctatct atatcgatct tccaatgcag agataattgc ttccatacct
    45061 tgatttttta ctgagttacc tatgcgagat atcattctcc actaaggtgc atcatattgt
    45121 ctatctaaca tgattaattc tccaataggt gtatactcct ccaaaaacaa catttaatgc
    45181 atctccctcg acaacataag ctaccatgtt tgctgtatat gtgtttgagt ctaattcata
    45241 agcagataaa tttactgtcg ctggattaaa tataatcgca ttcgtattta tagctactga
    45301 atttgctgct tcagcaccac cttttgaatg tccgatcatt gtgatttcaa tagtaggatt
    45361 tacgtggaca gccctcaccg tttaccctga ccattataga aatcgcttac ttttttgaac
    45421 tacgcaacaa aggatacaat gctcctaact tcactgtctc catttttgtc cactaaggaa
    45481 tataaaaaat aggcattaat tcatttaatt cggtcacaac acgttacctt ttaaatcatt
    45541 atactgtatc tcatacatta ttatcaataa actattataa aaatgcaaaa aatgctttat
    45601 caatctattt tgtaattggt cataatatca ttgaaatcat accctgtatc gatttttcct
    45661 atgaatctcg tctatttgtg attgacgaaa aagaggcaag acagacactg gaatggtaat
    45721 tttacccact attttgctcc aactatttgt aatcgtaccc acttttgaaa taccgagatg
    45781 atcattttaa ttgatatgct gattcccaag caaaacccca agaaattaaa tgctctatcg
    45841 catctagctt cttgggtttt gcttatataa attccaacat ttaaattttt tatgctgttc
    45901 ggtctcagaa atgccgtttt tgcttatatc acaatatcaa gatgtcaaat tattcgtgtt
    45961 gccgatgact ttgattaatt tttttcaaat tgtttattaa gactaatgct gtctttttgc
    46021 agataccaat gttcgaactt tttaacatta tcatatgatc ctgcaagttt taacttctta
    46081 ccattagatg ttatcaaagt tataattaac cttgcctttt tcccattagg atcttttatt
    46141 ttatgaattt tctgcaggta atctctccta atggcagcta tttttacctc atcagtgctg
    46201 ataaaccatt gatcatccac atacatactg cctgttatgt tgtgtttttc catttcttcc
    46261 gagaacgaaa agtttaaggc tcgttcttac ttccaaagtt gaatgagtcc atggattgga
    46321 cttagtgtga gaattgagaa caacaataag aatccatatc ctcccgcaaa catccctaag
    46381 gctatactgt ctctccatcc ataagtagga ttagaagaga aaagagtcac cgtttgtaat
    46441 aatatgaaaa ggaataatcc agcacaaaca ccaaagataa caactccaaa cattgaattt
    46501 ttatacaatg accatttatt tataggcttt tctttcattt tacttccccc tactgttctg
    46561 ttcacacgga atgaattcaa agttcataag ccctcctctt tgaacatatg aatacgactt
    46621 gctaactata ctttaaaaaa gcttgtttta agcggttata ggcgagttgt aatctctatt
    46681 catctcatta gcaaataact tgccgaaaag tctactgaaa ttaaagcaga tagtggaaga
    46741 attatttagc caggatagac actatttgtg aaacacatgt ctttactttt gaaattctat
    46801 gatgaaaagc cttggaatac agtagctctt aattggtaat actttttc
//
LOCUS       DAFGFZ010000006        45203 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000006 DAFGFZ010000000
VERSION     DAFGFZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.006"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            150..1340
                     /locus_tag="LR726_000274"
     CDS             150..1340
                     /locus_tag="LR726_000274"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL6215390.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1442..2395
                     /locus_tag="LR726_000275"
     CDS             1442..2395
                     /locus_tag="LR726_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL6215391.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2464..3813
                     /locus_tag="LR726_000276"
     CDS             2464..3813
                     /locus_tag="LR726_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6215392.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(3996..4319)
                     /locus_tag="LR726_000277"
     CDS             complement(3996..4319)
                     /locus_tag="LR726_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215393.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4448..5611
                     /locus_tag="LR726_000278"
     CDS             4448..5611
                     /locus_tag="LR726_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL6215394.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            5681..7030
                     /locus_tag="LR726_000279"
     CDS             5681..7030
                     /locus_tag="LR726_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL6215395.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7075..7506)
                     /locus_tag="LR726_000280"
     CDS             complement(7075..7506)
                     /locus_tag="LR726_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6215396.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            7894..10536
                     /gene="alaS"
                     /locus_tag="LR726_000281"
     CDS             7894..10536
                     /gene="alaS"
                     /locus_tag="LR726_000281"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL6215397.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            10736..11332
                     /locus_tag="LR726_000282"
     CDS             10736..11332
                     /locus_tag="LR726_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL6215398.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11454..12602
                     /locus_tag="LR726_000283"
     CDS             11454..12602
                     /locus_tag="LR726_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL6215399.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12640..13053)
                     /locus_tag="LR726_000284"
     CDS             complement(12640..13053)
                     /locus_tag="LR726_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL6215400.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13132..13563
                     /locus_tag="LR726_000285"
     CDS             13132..13563
                     /locus_tag="LR726_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL6215401.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(13662..13895)
                     /locus_tag="LR726_000286"
     CDS             complement(13662..13895)
                     /locus_tag="LR726_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL6215402.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14078..15748
                     /locus_tag="LR726_000287"
     CDS             14078..15748
                     /locus_tag="LR726_000287"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL6215403.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            15895..16281
                     /locus_tag="LR726_000288"
     CDS             15895..16281
                     /locus_tag="LR726_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215404.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16268..16966
                     /locus_tag="LR726_000289"
     CDS             16268..16966
                     /locus_tag="LR726_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL6215405.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            16971..17435
                     /locus_tag="LR726_000290"
     CDS             16971..17435
                     /locus_tag="LR726_000290"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL6215406.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17432..18343
                     /locus_tag="LR726_000291"
     CDS             17432..18343
                     /locus_tag="LR726_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL6215407.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18578..19117
                     /gene="pyrR"
                     /locus_tag="LR726_000292"
     CDS             18578..19117
                     /gene="pyrR"
                     /locus_tag="LR726_000292"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL6215408.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19131..20414
                     /locus_tag="LR726_000293"
     CDS             19131..20414
                     /locus_tag="LR726_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL6215409.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20427..21353
                     /locus_tag="LR726_000294"
     CDS             20427..21353
                     /locus_tag="LR726_000294"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL6215410.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21372..22652
                     /locus_tag="LR726_000295"
     CDS             21372..22652
                     /locus_tag="LR726_000295"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL6215411.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            22657..23733
                     /gene="carA"
                     /locus_tag="LR726_000296"
     CDS             22657..23733
                     /gene="carA"
                     /locus_tag="LR726_000296"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL6215412.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            23738..26923
                     /gene="carB"
                     /locus_tag="LR726_000297"
     CDS             23738..26923
                     /gene="carB"
                     /locus_tag="LR726_000297"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL6215413.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            26977..27753
                     /locus_tag="LR726_000298"
     CDS             26977..27753
                     /locus_tag="LR726_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL6215414.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            27750..28682
                     /locus_tag="LR726_000299"
     CDS             27750..28682
                     /locus_tag="LR726_000299"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL6215415.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            28679..29395
                     /gene="pyrF"
                     /locus_tag="LR726_000300"
     CDS             28679..29395
                     /gene="pyrF"
                     /locus_tag="LR726_000300"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL6215416.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29402..30031
                     /locus_tag="LR726_000301"
     CDS             29402..30031
                     /locus_tag="LR726_000301"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL6215417.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30019..30333
                     /locus_tag="LR726_000302"
     CDS             30019..30333
                     /locus_tag="LR726_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215418.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30371..31012
                     /locus_tag="LR726_000303"
     CDS             30371..31012
                     /locus_tag="LR726_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL6215419.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31055..31747
                     /locus_tag="LR726_000304"
     CDS             31055..31747
                     /locus_tag="LR726_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL6215420.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            31744..32646
                     /locus_tag="LR726_000305"
     CDS             31744..32646
                     /locus_tag="LR726_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL6215421.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(32804..34510)
                     /gene="efbA"
                     /locus_tag="LR726_000306"
     CDS             complement(32804..34510)
                     /gene="efbA"
                     /locus_tag="LR726_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL6215422.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            34700..35323
                     /locus_tag="LR726_000307"
     CDS             34700..35323
                     /locus_tag="LR726_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215423.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            35773..36822
                     /locus_tag="LR726_000308"
     CDS             35773..36822
                     /locus_tag="LR726_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6215424.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            36819..37700
                     /locus_tag="LR726_000309"
     CDS             36819..37700
                     /locus_tag="LR726_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215425.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            37703..38473
                     /locus_tag="LR726_000310"
     CDS             37703..38473
                     /locus_tag="LR726_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6215426.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            38752..39606
                     /gene="aroE"
                     /locus_tag="LR726_000311"
     CDS             38752..39606
                     /gene="aroE"
                     /locus_tag="LR726_000311"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL6215427.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            39784..40947
                     /locus_tag="LR726_000312"
     CDS             39784..40947
                     /locus_tag="LR726_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL6215428.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41064..41474
                     /locus_tag="LR726_000313"
     CDS             41064..41474
                     /locus_tag="LR726_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL6215429.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            41648..43876
                     /gene="topB"
                     /locus_tag="LR726_000314"
     CDS             41648..43876
                     /gene="topB"
                     /locus_tag="LR726_000314"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL6215430.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44047..44331
                     /locus_tag="LR726_000315"
     CDS             44047..44331
                     /locus_tag="LR726_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215431.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            44491..44727
                     /locus_tag="LR726_000316"
     CDS             44491..44727
                     /locus_tag="LR726_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL6215432.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            44724..45123
                     /locus_tag="LR726_000317"
                     /pseudo
     CDS             44724..45123
                     /locus_tag="LR726_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        14813 a         7857 c         9822 g        12711 t
ORIGIN      
        1 aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat
       61 caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg
      121 caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag
      181 agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa
      241 gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca
      301 attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa
      361 cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc
      421 aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg
      481 gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat
      541 cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca
      601 tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa
      661 gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca
      721 ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg
      781 atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat
      841 taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg
      901 gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc
      961 tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga
     1021 tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag
     1081 aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt
     1141 tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg
     1201 ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga
     1261 ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa
     1321 aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat
     1381 gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat
     1441 catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg
     1501 gctacgtcaa gaactaggca gtggaaatgt cattgcgaca gatatacgca tgcctgaagt
     1561 cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag
     1621 catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt
     1681 tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt
     1741 ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg
     1801 tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta
     1861 tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt
     1921 agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg
     1981 tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag
     2041 tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat
     2101 tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc
     2161 ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt
     2221 tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc
     2281 actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt
     2341 gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa
     2401 gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa
     2461 gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa
     2521 cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg
     2581 gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt
     2641 tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca
     2701 gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca
     2761 gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc
     2821 ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt
     2881 gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa
     2941 gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc
     3001 gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca
     3061 ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca
     3121 tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat
     3181 ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat
     3241 gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt
     3301 gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg
     3361 atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt
     3421 atggcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact
     3481 aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc
     3541 ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg
     3601 gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa
     3661 gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca
     3721 agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt
     3781 atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact
     3841 gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa
     3901 aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta
     3961 aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact
     4021 attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga
     4081 agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta
     4141 tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa
     4201 ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc
     4261 gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc
     4321 tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc
     4381 gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag
     4441 gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt
     4501 agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct
     4561 agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt
     4621 ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga
     4681 gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga
     4741 acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt
     4801 tttattactc ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa
     4861 aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact
     4921 ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac
     4981 attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag
     5041 aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt
     5101 ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt
     5161 ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga
     5221 agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa
     5281 aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat
     5341 gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct
     5401 tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc
     5461 tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag
     5521 aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg
     5581 ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata
     5641 atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt
     5701 ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga
     5761 ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca
     5821 aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga
     5881 aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta
     5941 tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt
     6001 cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt
     6061 agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac
     6121 cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga
     6181 agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac
     6241 tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga
     6301 aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg
     6361 taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat
     6421 cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg
     6481 cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg
     6541 acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat
     6601 cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt
     6661 tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata
     6721 ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc
     6781 aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg
     6841 agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa
     6901 aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa
     6961 agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc
     7021 acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc
     7081 tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt
     7141 atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt
     7201 ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc
     7261 ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt
     7321 cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg
     7381 ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa
     7441 aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt
     7501 tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc
     7561 gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca
     7621 gtgatcaata gaatgcgtat actaaataag tacgaaaagg atatgatttg ttttttaaga
     7681 ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt
     7741 ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt
     7801 acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt
     7861 tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt
     7921 cgacaaatgt ttcttgattt cttccaatct aaagaacata cgattgaacc tagtgcgtct
     7981 ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa
     8041 aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct
     8101 attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag
     8161 atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg
     8221 gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt
     8281 tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat
     8341 cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat
     8401 acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa
     8461 aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa
     8521 ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg
     8581 atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac
     8641 ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa
     8701 aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt
     8761 gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg
     8821 cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt
     8881 ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa
     8941 gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa
     9001 ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta
     9061 gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa
     9121 gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat
     9181 gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct
     9241 gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca
     9301 gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt
     9361 gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg
     9421 gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa
     9481 aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga
     9541 caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca
     9601 gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct
     9661 ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact
     9721 tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca
     9781 gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt
     9841 ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg
     9901 tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa
     9961 tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa
    10021 ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa
    10081 ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag
    10141 gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat
    10201 ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat
    10261 cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta
    10321 gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa
    10381 gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt
    10441 ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca
    10501 cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac
    10561 aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct
    10621 catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag
    10681 ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa
    10741 tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt
    10801 acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt taacaattat
    10861 ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg
    10921 cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga
    10981 tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt
    11041 agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc
    11101 ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt
    11161 attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac
    11221 cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac
    11281 accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc
    11341 ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc
    11401 gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta
    11461 agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag
    11521 cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc
    11581 ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt
    11641 catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc
    11701 cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt
    11761 atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct
    11821 ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag
    11881 gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa
    11941 cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat
    12001 gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt
    12061 atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc
    12121 cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc
    12181 atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag
    12241 tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact
    12301 ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg
    12361 aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc
    12421 ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg
    12481 tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag
    12541 aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt
    12601 aaatgagaaa ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt
    12661 taattgttta tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg
    12721 caaccattgc aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta
    12781 gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct
    12841 gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt
    12901 ttttaaaaag acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag
    12961 ctgttcttgt ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc
    13021 ttttgtagag gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa
    13081 ctaatgttat ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa
    13141 aatattcgat ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc
    13201 attctgtttt ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat
    13261 tttgttttag gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta
    13321 aaatcaaact ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc
    13381 ggacagattc gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag
    13441 aaagccccat ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt
    13501 gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc
    13561 taattttaaa aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac
    13621 gtacggttca tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta
    13681 tcggtagtct gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg
    13741 atgatctcaa attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc
    13801 gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt
    13861 ttgttgtcaa accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct
    13921 gatcatagcc ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta
    13981 attttatgtt ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat
    14041 attttgttga ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc
    14101 acaagaaaca gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga
    14161 cgatttggaa ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc
    14221 aaaggataac gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg
    14281 cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa
    14341 atctgttatt gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc
    14401 tgcaggcgga ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg
    14461 tgacatgcat gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat
    14521 tcatcaagga aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga
    14581 tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt
    14641 tccaagagaa gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct
    14701 tgcggcagac cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt
    14761 tgaccgtcag cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct
    14821 gaaagatgca atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca
    14881 cgggggaccg tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc
    14941 attgagatta gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga
    15001 gaaattttta gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt
    15061 cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga
    15121 gaatcttgat gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga
    15181 acaatatcag ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga
    15241 actcacactg ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt
    15301 atgggcagat ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga
    15361 tagaaaagaa gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac
    15421 agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca
    15481 aaaacaaata gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa
    15541 aacacagtat tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat
    15601 cacgatccgc gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga
    15661 tgttatgacg atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa
    15721 tgacggagaa gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt
    15781 attttgacta cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc
    15841 attctttttc tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa
    15901 acgacattta taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc
    15961 aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg
    16021 ccctttacga tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa
    16081 gcaaagcctg cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta
    16141 tttctagcaa ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca
    16201 gttattgtgt tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag
    16261 gaaaattatg gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat
    16321 gagagacgag atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc
    16381 tcaaagaaaa caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt
    16441 aaggaatgcg atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga
    16501 ttggacagtc aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct
    16561 gcctcgattt gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt
    16621 aaagatagta gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt
    16681 agcattgatt actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc
    16741 ggttgattta acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa
    16801 ttatcatttt gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca
    16861 agggactttg gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg
    16921 gatcgtacga cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta
    16981 tttattggat attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca
    17041 ctgataatct agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc
    17101 atattcgaaa cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta
    17161 tagtgacact cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag
    17221 gtaatcgctg gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta
    17281 tcgaccgtat tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc
    17341 cgatttttaa cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata
    17401 ttattttaga tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca
    17461 tgaatctggt aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca
    17521 aattcagctg tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa
    17581 ttacaaagta aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga
    17641 tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat
    17701 aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt
    17761 caatgcgctg atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg
    17821 tattgttcac cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca
    17881 ggcacacgaa tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc
    17941 tttagtccat ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc
    18001 gaaggaaaac cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt
    18061 tactgtattg gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag
    18121 aacacaccag atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt
    18181 atacggaccg aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg
    18241 ttttacgcac ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt
    18301 tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag
    18361 taattaacaa cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct
    18421 aagaagggtg ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag
    18481 tatagctatc agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc
    18541 aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga
    18601 tcaggtaacc atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca
    18661 tagtatccaa gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag
    18721 aattgccgaa cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat
    18781 cactttatac cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga
    18841 catcccagtt tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg
    18901 acgaacgatt cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc
    18961 attagcagtc ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg
    19021 gaagaacatt ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg
    19081 acaagatcgc atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga
    19141 aaaaaggatt tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac
    19201 attgggtagg attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc
    19261 ccatcttagt aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg
    19321 tgtacttatt tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca
    19381 ttgcagcgat gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag
    19441 tcacaactgg attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat
    19501 ggctggataa aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg
    19561 gacttgcggc aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt
    19621 atattgctgt agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag
    19681 gttttctcgg attgattccg attttactag gtattattag cggatacatc gtagccttgc
    19741 tgtttggaat cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact
    19801 ttgagattcc ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg
    19861 cacccatcgc ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac
    19921 taactaaacg taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat
    19981 tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca
    20041 ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat
    20101 ttgcagtcat tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac
    20161 cagtcatctc aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga
    20221 ttttaatcga gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta
    20281 tcttagtagt aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa
    20341 tggcattagc aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa
    20401 gcgaagaaaa ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc
    20461 atttattgac cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc
    20521 aggaattcaa gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc
    20581 tctttttcga aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag
    20641 ggctagaagt gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat
    20701 acgatacagt actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg
    20761 atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg
    20821 gaggagacgg aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg
    20881 aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc
    20941 gcgtagctaa atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag
    21001 gtcctagaga atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg
    21061 aactactaga tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg
    21121 gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg
    21181 cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag
    21241 aattagctga ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg
    21301 gagtattcgt cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg
    21361 aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga
    21421 cgacaccagc agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct
    21481 cagaagccga atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag
    21541 tggacgtcca tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg
    21601 ggactcgctc agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc
    21661 ctgtacctga tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag
    21721 ttgtgaaggt attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg
    21781 atcaagaagc actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc
    21841 aaacagctgg gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag
    21901 tagcccatac agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg
    21961 cagaagaact tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag
    22021 atctgttgct agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag
    22081 aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt
    22141 caccccatca tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa
    22201 tgaacccgcc gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg
    22261 gtacgatcga ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa
    22321 gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata
    22381 ctcattttgt agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta
    22441 aaccggctga gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg
    22501 tagcagtttt tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag
    22561 gggagaatac accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca
    22621 acggaaaact tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa
    22681 gatggaacag ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc
    22741 gtttttacaa caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt
    22801 caaatcatta catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat
    22861 gaatcgattt ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca
    22921 aactggcgaa accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata
    22981 tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct
    23041 agtatagttg atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt
    23101 ctagcaacca atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca
    23161 ggtcggaaag tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt
    23221 aaaagaaact gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg
    23281 ctatcaccag acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa
    23341 gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt
    23401 caccagttat ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga
    23461 ggattaaatc acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat
    23521 catggctatg cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta
    23581 gaagtaaatg acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg
    23641 cagtttcatc cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt
    23701 atggaaatga tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa
    23761 gaaaattatg gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta
    23821 cgcaggaaca caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa
    23881 ttctaatcct gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc
    23941 gatcacactt gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc
    24001 aactttaggc ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt
    24061 agaagaattg aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga
    24121 tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat
    24181 cgtcaataca gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt
    24241 tcgtccagcc tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt
    24301 acgtatcgta tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg
    24361 aagtattgca ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc
    24421 aatcgttgtc tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat
    24481 cgtatttgcc cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc
    24541 tctttcgatt attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga
    24601 tccacatagt tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc
    24661 gttagcaagt aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg
    24721 attaacatta gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc
    24781 tgcattagat tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg
    24841 agaacggctt ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa
    24901 tatcgaagag tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga
    24961 acttcctgag ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca
    25021 agatgatcgc ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt
    25081 agcggatttg acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat
    25141 cgaacagcag ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa
    25201 cggttttaca gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca
    25261 gatgagaaaa gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga
    25321 attcgaatca aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg
    25381 atcagaaaag ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt
    25441 tgaatttgac tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc
    25501 aatcatcatg aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt
    25561 atattttgag ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat
    25621 aggagtaatc gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc
    25681 tgctggtgta aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga
    25741 tttattcgag caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac
    25801 aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc
    25861 aagctatgta ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga
    25921 ttacatggaa catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt
    25981 gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg
    26041 gatcatggag catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc
    26101 acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc
    26161 attaggtttg aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt
    26221 ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac
    26281 aggaattcca atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact
    26341 tggttatgaa aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt
    26401 ttcttttacg aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg
    26461 agaagtaatg ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc
    26521 tggattacac ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga
    26581 agaagcacta gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa
    26641 acatacagct gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc
    26701 tgaggaagca gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt
    26761 tgtcaatacg attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag
    26821 agaagcagtt gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt
    26881 tcgagtgatg gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa
    26941 gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc
    27001 gttcagcaaa aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta
    27061 aaagaaatgc agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta
    27121 ttacgccgac cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt
    27181 tatcgggtgg aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta
    27241 gatgtcatgg gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa
    27301 gtatttataa tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta
    27361 gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat
    27421 tatgaaaaag aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac
    27481 ggtatgcacg gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt
    27541 tttgcatgtg gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa
    27601 aatgtccagt tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc
    27661 gtttgtcata cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga
    27721 ccgattttta aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc
    27781 ctggcctaga attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag
    27841 aatacggaaa atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc
    27901 caaatgcccg ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga
    27961 atgcaattgg tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat
    28021 tagaggagta caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact
    28081 atgtagaagt ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca
    28141 tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt
    28201 ttcaattaac acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc
    28261 caaatgtaac agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt
    28321 ttacgatgat caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt
    28381 tagccaatca aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga
    28441 ttcaccaagt agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag
    28501 tggatgatgt tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga
    28561 atttcaccga tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg
    28621 aactaggcat cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat
    28681 gaatcaacta gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga
    28741 agactttctt cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt
    28801 gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt
    28861 tttagatctg aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc
    28921 aaagcttggt gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc
    28981 ggcaatgcga ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc
    29041 tatcactcag cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt
    29101 ttctttagaa aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg
    29161 tgtggtttct tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt
    29221 ttgcttaact ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt
    29281 cacaccttct caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac
    29341 tcagtcaact aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa
    29401 aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa
    29461 tccaagtaat ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg
    29521 aatcacgatg agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat
    29581 caaagaagct taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca
    29641 tgccgcatgg gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa
    29701 agatcatggg aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt
    29761 gatcgaggat ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag
    29821 agaaggtgcg gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa
    29881 agcaaatttc gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat
    29941 tgaagcagct ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg
    30001 gaaaaaagat cctgaaaatt ggcagattta agcgcgcttg atgaagatag agaaatccga
    30061 tttgtgtctc cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc
    30121 atcttttcat caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa
    30181 ttcatttttc taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt
    30241 atatatagat tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga
    30301 aatgttgcga atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga
    30361 ggtaactttt atggggttga atgaatttat tttaggattg aacaatttag agacgatcac
    30421 aagggcacct ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt
    30481 agcatctatt gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg
    30541 gaaactgctc tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat
    30601 caaaacgccg gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga
    30661 aaagatgacg gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg
    30721 ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga
    30781 taaggttgac cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt
    30841 ttataaggag atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc
    30901 tgttcagtat ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa
    30961 agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata
    31021 tgagatgcag ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg
    31081 agacaaaggt ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt
    31141 tccattgcag tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac
    31201 gctttccttt cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca
    31261 tttagtacct tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac
    31321 agatattcat ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga
    31381 atttcatttt gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca
    31441 gctaatggta caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga
    31501 aaaacatgag cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca
    31561 ttatattggc tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga
    31621 tcatgtagga aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc
    31681 gaacaaccga ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga
    31741 gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga
    31801 gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta
    31861 gaaaccgagt tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa
    31921 gaagggcaag acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa
    31981 actcactatc taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat
    32041 ttcttaggtc ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt
    32101 cggatcatgg agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag
    32161 atcggcatta tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa
    32221 ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga
    32281 catccattag ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt
    32341 cgttttacac aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct
    32401 gatcaagaaa cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa
    32461 actgatgctt acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg
    32521 ttaatccctt tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt
    32581 ccgctcagtg aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag
    32641 agctaagaaa agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt
    32701 tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg
    32761 ttgtgggaaa gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca
    32821 tagctgtaat tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa
    32881 cataaccagg cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg
    32941 gcacttgggc agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa
    33001 ttgtttcatc tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg
    33061 catgaagcca atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat
    33121 tgtttttacc aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg
    33181 gtttcttacg tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt
    33241 cttctttaat tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt
    33301 caagatattg gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt
    33361 tcttcagctt ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg
    33421 gatctagtgc tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt
    33481 ttgcgccgcg tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat
    33541 tctcagcgtt ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc
    33601 gttttagttc attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt
    33661 tctctgcttt ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta
    33721 cagcagatgc ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt
    33781 ccgtttcgta aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata
    33841 caaccatctt ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga
    33901 aaccgattcc ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc
    33961 gctgaaagat tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag
    34021 caacatattc tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt
    34081 tgatcgcatc cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc
    34141 ccattaattc aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa
    34201 agtggaaatg gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat
    34261 ccaaatattt tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga
    34321 tttgggtaat ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt
    34381 tgccgcgtga acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga
    34441 tccggccaga tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat
    34501 caaaggacat ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa
    34561 gaagaaaaac caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt
    34621 acataaaaaa cagtgtttct atcgttgaaa tccaaatttt taggtataat aaaaaatagg
    34681 agaaaaagta gaaaagaagg tgtttgttat ttgggaagta gttaaatcga gagtacaaga
    34741 aatgaatgaa catttctcag tgtcattcgc agaaaatcca agtaagtatt ttcgacgtct
    34801 tgtttttctg ctcatcttat cattgctgtt aacagttgca atgtttttat tgccggaaaa
    34861 aatccgagaa gagcaagtaa atagtctcgt ttattcgtcc ataccaacca gcaaattgaa
    34921 accttttgat tacagcaaag ctgatcagaa aatcaaacaa agccgggcga tatcggtgat
    34981 gttcagcaaa ccgaacggga aaacctatca ggacatgctt gatgttttta atgatcctga
    35041 gcttatggaa gaattgaatc gaccgatctt ttattatccg atcgtttatg atgtacatga
    35101 actggaacaa aaatataaca tacgaacgga tgaagtgaca tttattttct ttgatggagg
    35161 aaaagaagca aatcggataa ctgcgggtaa gggaggaata actgattttg ataaggagtt
    35221 gatccctgaa ctgaatcgac ttccgttagc caatatcaaa caattagaag aagaattgga
    35281 tagaacggga gtaacttccc aaaattccga gattgctaat tgagacattg gcgcgatgaa
    35341 acggtgggtc tatatagcgc tatttttaat tatcagaaca agtgatgttt gatcttcaaa
    35401 ataacaaata aaaaattaaa aaatatagtt gacgaaattc aaaaattcga ttagacttaa
    35461 atcaattctt aaggaaaggg tgacctgtaa tgaacttatc aaaacgtatt tcagcagaaa
    35521 acaaaaaaaa ttggcgcagc tggcgtagat aagttgtatt aatggtgaga ctttcccata
    35581 gatacaactt tttagagtgc atttctaaag cttgtatcta tgctagacat gaacattgac
    35641 tgccgcatag attagaatct atgcggtttt ttgtgtaaag acaatgaccg ctttggccat
    35701 tgtctttttt tgtagactgg atacttgcct gattataata aataaaaaga ttgactttgc
    35761 aggaggaaaa aaatgaaaaa caaacgatta ataacagttg tagtaattat tttagtttat
    35821 ttagcaggtg catttattta tggaaaagcc tcaggcgaaa aagaaagtac ttcgacgaac
    35881 cagacaaaaa aaacaacagt tgttggggta ttgcaatacg tgagccatcc ggcattagat
    35941 gagatttatc gaggaatcaa agatggtctg aaagaagaag gattaaaaga aggagaaaat
    36001 cttgagatca aatttcaaaa tggacaagcc gatcagagca agctagccac aatgagtcaa
    36061 cagctagtac aatctgatcc agatgccttg attggaatcg caacacctgc cgctcaatca
    36121 ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg ctgtaacgga tcctgtgggg
    36181 gctggattag tcgcttcttt agagaaacct ggtggtaatg taacaggtgt ttctgatcag
    36241 ccgcctgtta cttcacaaat caagcttgct tctgatttgt tgccaaatgc aaaaaaagtc
    36301 ggaatcttgt atgcttcttc agaggataat tcaaaatacc aagtagcaca agcagaagaa
    36361 gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg tgccttccac taatgagatt
    36421 gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta tctatgttcc attagataat
    36481 accattgcca atgcgatgca aacagtggta aaagaagcaa acaaagcaaa tatccccgtc
    36541 attccatctg tggatacaat ggtggaacaa ggtggactag caacaatcgg gatcaatcaa
    36601 tatcaattag ggcttcaaag cggtaagatg gcggcagaac ttgcgagtgg aaaagaaaaa
    36661 ccggaaacga cgcctgttta tacatttgat caaggggata cagtgatcaa tcaatctcaa
    36721 gctgatcgct taggaatcat tgttccccaa tcgatgaagg aaaaagccaa aatcattaca
    36781 gatgactcgc agcaagaaac atctaaggag gatgacaaat gatcgtatca gctatttcac
    36841 aaggcatgct ttgggcaata ttaggattag ggattttcat gacttatcgg atcttagact
    36901 ttcctgatat gacgacagaa gggtccttcc cattaggtgc tgctgtctgt gtgacagcta
    36961 ttactcatgg ggtagcgccg attgtagcta cattattagg cgtaggtgct ggcatgatag
    37021 caggactcgt tacggggtta ctttatacaa aaggaaagat accggttatt ttagcaggga
    37081 ttttagtgat gtcaggattg aactcagtta ttttgtttgt gatgcgaaca ccaaatcttt
    37141 ctttattgaa tcatccgatt ctacaagaca gcttccatcg ctttgggtta cctgattact
    37201 ttgacacagt ccttcttggc ttcatctctt tgggcatctt gattgctgta ctgctattct
    37261 tcttcaatac agatttgggt caaggctata ttgcgacagg agacaacgaa acgatggctc
    37321 ggtcgttagg gataaaaacc gatcgtatga aaattttagg tttaactttg tcgaacggtg
    37381 tcattgcatt atccggtgcg ctgattgccc agaacgacgg atatgcagat gtaaataaag
    37441 ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat cggtgaagtg atcttccaag
    37501 aattgacgct agcagaaaga ttgattgcta ttgttgtcgg cagtatcatt tatcagctat
    37561 tgattttagt agtaatcaaa ctagggtttg acacgactta tctgaagttg ttctccgcta
    37621 tcatcttagc tatttgtctg atgatcccgc aattgaagaa agcactgaac ttaaaaacgg
    37681 gttttgagaa ggaggcatag taatgactga tatattgaca atcaaaaatg gcagaaaagt
    37741 cgtaaacaat ggaatgaatg aagagaaagt actattggat gaaattaacc ttacaatcag
    37801 agaaggggac ttcatcactg tccttggcgg gaatggtgct ggaaaaagca cactgttcaa
    37861 tacgatcgct ggtacgcttc aactcagtca aggatcgatc tcttttaaag atcagctgat
    37921 cacaaaagat tcagaagaaa aaagagcacg attcctttct cgagttttcc aagaccccaa
    37981 gatgggaacg gctcctcgga tgacagtagc agaaaatttg ctacttgcac aaaaaagagg
    38041 tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa aaagaagatt ttttcagact
    38101 atgtcaagaa gtcggtaatg gcctagagca acacttggat actccagcgg gtgaattatc
    38161 tggtggtcaa cgccaagcat taagcttgct gatggcgacg atcacagctc ctgagttact
    38221 attattagat gaacatactg cagctttaga tcctaaaaca gctaaagcat taatgcgatt
    38281 aacaaatcag cggatcaatg aacaaaaatt gacttgtctc atgatcacgc atcgaatgga
    38341 agacgccttg aaatacggaa atcgtctgat cgtactccaa aaagggaaaa tcgttcaaga
    38401 tatttctgaa aaagaaaagc aacatttgac aatgggcgac cttttgcagt tctttgaaga
    38461 agtagtggat tgatttcgac agaaaaattc ctttcagtag aaggacttga aaactcattg
    38521 acatcttcct ttgattcatc ctatacttaa aacaataaac aattaaacat atttgggtgt
    38581 tttgtaggat cacgtagtca atgattttaa caattaaata gaatattctt tgctctgaca
    38641 agtaaacgat gagaaaagaa tttgctactt tgccacctag acgtttcgta ctttttcgta
    38701 tcaaacgcta agtggctttt tttattgaag taagaacaaa ggaggaataa gatgatttct
    38761 ggacaaacca aattagctgg gctctttgct gatccggctt ctcacagttt atccccattg
    38821 atgcacaata ctgcttttga agcaaacaac atcgatgcgg tgtatttagc tttccaagta
    38881 aatccaatga atttgaagca agcagtcgaa agtattcgaa cttttgatat gttaggtgta
    38941 aatctttcga tgccgaataa aacagcggtt attccttatc tggatgaggt aagccaggaa
    39001 gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa agggtcgttt gattggctat
    39061 aatactgatg gtgcgggctt catgcaatcc gtgcaagaag ctgggatttc gatcaaaaag
    39121 aaaaaaatga ctctactggg agctggaggt gcagcaaagg cagtcactgt tcaagcagcg
    39181 ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca accaaacatt cgctcaagta
    39241 aaagaaacat tcgaacaact cagctatcga acttcttgta agataaaagt tcgtgacttt
    39301 gccgatacgc atcaattaag aaaagacttg aaagaaagct gtcttctagt caatgcaaca
    39361 gatatcggga tgggaaagaa aatcaatcag tcaccgctct tagatataag catgatgcat
    39421 gagaatctag cggtttttga tttgatttat gcacctagag aaacacgttt attaaaagaa
    39481 gcaaaaaaag taggagcaaa aggatacaat ggacttggca tgttgatcta ccaaggagca
    39541 atcgcgtttg aattatggac aaaggaaaag atgccgatcc atgtgattga aaagctgttt
    39601 gattaaaaaa ggataataga taaagatctt ttattctcgc ataaacggtc tcaagaaaag
    39661 taatccttta tgtatcgaag catttatttc gttatttttt attaaattat caaaaaatga
    39721 taggcttttg tcgattggtt atcgtatcct ttatttatca gtaagaaacg aggaggaacc
    39781 acaatgagtc ggatggatcg ctataaggac atccataaga aggcgaaacc tctgaagcgg
    39841 gatacgggga gtatttttgc tagaagagaa cgaaaacgaa cgcgtatgga aaatgaggaa
    39901 gatgctaccc gtatttatga aagagagcag cattcatttg aacatttgaa ccaatacgag
    39961 caacagatat acagagatgc accaagagca gaagaacaac aaaaaaataa aaaatttttc
    40021 aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa agcgatcgtg gaaaaaaatc
    40081 attctatcga ttttgcttgt tttgatcctt tattcaggta tctcattttt tattggtaaa
    40141 tatgtagctg aacatgacac aagcttagag gcagcgactt cagaaacctt tcatgggcaa
    40201 aaagcgacga atggtgcgca taatattttg atccttggaa gtgatacacg cggagaagat
    40261 gccgggcgag ccgatacgat catggtctta cagttggatg gtccttccca taagccgaaa
    40321 ctgatttctt ttatgcgtga cagcttcgtt acgatcccag gggtaggaca aaataagatc
    40381 aattcagctt atgcatacgg aggagccgat ctagtgcgtc aaactttagt agaaaatttt
    40441 ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat cgtttgaaaa agtgatcgat
    40501 gccttattca tgaacggagt gaagatcgat gcagaaaaag atctgaatct ggatggtgta
    40561 gatattaaaa aagggggcca gaagatggat ggacatgttc ttttgcagta cgcacgtttc
    40621 cgaatggatg aacaaggaga ctttggccgt gtcagacgcc aacagcaagt aatgaacgct
    40681 attttcagcc aattgaagaa tccattgaat ctgatccgtg caccttatgc agctggtaaa
    40741 gctttaggat atacatccac agatgtcact gcttggttct tgacgaaaaa cctcttttcg
    40801 attgcacgag gagctggtgg cattgatcga ataagtgtgc cagtagacgg ttcgtggaat
    40861 tatgggcaaa gtgattatgc tggcagtatt cttgaaattg ataatcaaat gaatcaatct
    40921 gctattacag attttttgaa taaatgatca taaaaaagat caagaagaag ctgcgcaagg
    40981 cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa cgctttctta tggtagattt
    41041 aaccaagaga ggagctgttt acattggata tctatctgaa cttcaaaaat gagtcgcgtg
    41101 aagccatcgc tttttatgaa aaagtttttc agactgaagc atcaggtatc accacatacg
    41161 gtgaaatgcc tggaatagaa caaatgaact tgccagaaga agcaaaaaat ctaatcacga
    41221 atgcacggct gaacatttat gatacattag ttatgtttgc agatacgcca gaaggaattg
    41281 gtcctgaatt agttgtggga aataatgttt ctttagtcat ccagacaaga aatgaagaag
    41341 aaacgaagcg cctatttgaa agcttagctg agggtggtaa gattgagatg ccactggatg
    41401 ctgttccgtg ggctcagttg tatgggatgg taactgatcg atttggcatc aactggcaga
    41461 ttaaccaaga gtgacagcta aagatgaata ttttctggaa acatcaagca gcaaacggat
    41521 catcgtttgt ttgcttgatg tttttgtgtc tatttcgact atacggtatc ttttttttat
    41581 tgccagatgc gaaaatatgt tccataatag agaagtccgc aaaaaattaa gtaacggagg
    41641 tcagaagatg aagacggtga tactagctga aaaaccttca caagcaaaag cctatgccga
    41701 aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa atcagcgatc cattgtttgc
    41761 tggtgaagta accatcactt atggttttgg acatttggta gatatggttg ctccaggagc
    41821 ttatgaagag cgttgggcaa aatggtcttt agaaaatttg cctattttcc cagaaacctt
    41881 ccgttacgaa gttccaatgg acaaacgtgc acaattttcg atcgtaaaac gagaattgca
    41941 gtcagccgat accatcatta ttgcaacaga tggtgatcga gaaggtgaag ccatcgcgtg
    42001 gtcgattatt cgacaagcaa acgcattttc taaggaaaag aactatttac gtttatggat
    42061 caattctctt gaaaaagaag caatctatga aggttttaag aatctccaac ctggaaagaa
    42121 ctattttcca aaatataaag aagcgcaagc aaggcagaac gctgattggc tgattggaat
    42181 gaatggaagt ccattatata gcttgctact tcaacgaaaa gggattgatg gcagtttttc
    42241 tcttggtcgt gtacaaacac cgacactcta tatgatttat cagctgcaag aagaaatcaa
    42301 acacttcaaa aaagaaacgt actttgaagg agaaggttta attacgacaa acaaagggca
    42361 gttttctgga aaaatcgtac caaaaaaagc atttaaaacc caagaagagt taacgaagga
    42421 aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga cggattcttg aagttacgaa
    42481 aaaggaaaag aggttgcata gtccacgttt attttcgcta tctagtttgc agacaaagat
    42541 gaatcagcta atgaaagcaa gtgccaaagc tacacttgat gctgctcaag gattgtacga
    42601 aagcaagttt ctaagctatc caagaacgga ttccttttac attacagaaa atgagcatca
    42661 gtatctggtt aaacatctgc aaaattataa aaaattttta ggaatagaag aagtacagac
    42721 aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa aaagttcaag aacatcatgc
    42781 aattattccg acaaagacga ttccgactcc tgcacgcttt gcgaagcttt ccaaattaca
    42841 gcaagcaatc tatttgcaag ttttgcgcac tactgtggct atgtttgctg cagattatat
    42901 ctacgaagag acgaccgtaa tgacgggcgt acagcaatta aagctaaaaa gcatcggaaa
    42961 gatcccatta cagcaaggtt ggcaggaaat cttacgcatc aagtcaaaaa agaaagaagc
    43021 tcagactctt cctcctatcg tagaaaatga agaagcagaa gtagaactga aaagtatgga
    43081 gaaagaaaca caacctccaa aaccctatac cgaaggaacg ttgatcacag caatgaaaac
    43141 tgcgggaaaa acgctggata atgatgaagc gcaagaaatt ctcaaagaag tagaaggaat
    43201 agggacagaa gctacacgag ccaatatcat tgaaaatctt aagcagaagc attacatcga
    43261 agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc actctttgta aggcagtagc
    43321 caatgaacca ttactgacga gcgcagaaat gactgcgcgc tgggaagggt acttgagaaa
    43381 aattggtaaa caagagggga caccatctgt ttttttagag aatatcaaaa aattcatcct
    43441 acatttattg gcagaagttc ctaatcaaat cgacaaggta gacttttcag atgagattag
    43501 cgggacacgt cagataaaag cgatggagaa gaaaaacgat caattaggaa gatgtccaaa
    43561 atgtaaaaag ggaatagtca tgctttatcc taaaatcgct acatgcttga atccggaatg
    43621 cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg actaaaacga atctcagaga
    43681 tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt tttactggta aaaaagggaa
    43741 attcgatgca gttttagtat taaaagaaga tatgacaatc ggtttttctt ttccatggat
    43801 cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa acatcaagaa aaaataatga
    43861 aaacgaagta gagtaaatga aaacaatgaa atagcggaat atttattccg aaaagtgaga
    43921 atataaatac caataaaact aaaaaatatc agtgaatcaa gcaaaaacgt tggggtaggc
    43981 tgattttata tagatagcaa ggagtttttt tatttgaaaa aagtgcattc atttttaaat
    44041 tatcttatgg gagcaatcac gattgtggct gtgtaccaag catttctaaa gtatcctgat
    44101 tacgggacga agttgttgtc attgacgcac aatcaaacag catattcacc aacgttgatt
    44161 ttatcgactt tgactgcagg agctaaaagc gctacttatt tttttcataa aacgacaagc
    44221 atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg tggtagcggc tatgatcatt
    44281 atttattttc taccagttat tacacaagta tttcatcaat attttggtta agaatacgag
    44341 agtttgtgac aaaagtcatg tcacaaactt ttttctttgt cattttttcg taagaaaaag
    44401 caaaataaaa gtatatacat tgattaaaag tatatacttg gatataatgt acttactata
    44461 gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta taaaattaga
    44521 aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt catgggtgtg
    44581 cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg aatgatcaaa
    44641 gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca atatgaagac
    44701 gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt atcaaaatca
    44761 acgcgaaagt tattttaccg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat
    44821 gcattagata tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatcct
    44881 gaaatctatg aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat
    44941 aatggcaata aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt
    45001 ccagttgctt tttctcgtca agaagctgtt gattttatca taatgattac cactagttca
    45061 ttggatttcg attcactaaa gtccaagata accagttatt tacgacaaac cgcaataaaa
    45121 tgaacgaatt aaaaacttta tttctcaatt agatcagcat gtcattaagg aatatgctga
    45181 tttttttatg tttaatcata caa
//
LOCUS       DAFGFZ010000007        44847 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000007 DAFGFZ010000000
VERSION     DAFGFZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.007"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            212..604
                     /locus_tag="LR726_000318"
     CDS             212..604
                     /locus_tag="LR726_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL6215433.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LR726_000319"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LR726_000319"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL6215434.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LR726_000320"
     CDS             3165..4775
                     /locus_tag="LR726_000320"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL6215435.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LR726_000321"
     CDS             5067..5936
                     /locus_tag="LR726_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL6215436.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LR726_000322"
     CDS             6101..7372
                     /locus_tag="LR726_000322"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL6215437.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LR726_000323"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LR726_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL6215438.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LR726_000324"
     CDS             8763..9026
                     /locus_tag="LR726_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL6215439.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LR726_000325"
     CDS             9471..9920
                     /locus_tag="LR726_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6215440.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LR726_000326"
     CDS             9977..11395
                     /locus_tag="LR726_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6215441.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LR726_000327"
     CDS             11395..12255
                     /locus_tag="LR726_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL6215442.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LR726_000328"
     CDS             12429..13202
                     /locus_tag="LR726_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL6215443.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LR726_000329"
     CDS             13349..13714
                     /locus_tag="LR726_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL6215444.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LR726_000330"
     CDS             complement(13862..15070)
                     /locus_tag="LR726_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6215445.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LR726_000331"
     CDS             complement(15089..15367)
                     /locus_tag="LR726_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215446.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LR726_000332"
     CDS             15521..16216
                     /locus_tag="LR726_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL6215447.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LR726_000333"
     CDS             complement(16658..17455)
                     /locus_tag="LR726_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL6215448.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LR726_000334"
     CDS             complement(17452..18636)
                     /locus_tag="LR726_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL6215449.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LR726_000335"
     CDS             complement(18661..19908)
                     /locus_tag="LR726_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL6215450.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LR726_000336"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LR726_000336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL6215451.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LR726_000337"
     CDS             21888..22202
                     /locus_tag="LR726_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL6215452.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LR726_000338"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LR726_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL6215453.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LR726_000339"
     CDS             22887..23531
                     /locus_tag="LR726_000339"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL6215454.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LR726_000340"
     CDS             23546..23875
                     /locus_tag="LR726_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL6215455.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LR726_000341"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LR726_000341"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL6215456.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LR726_000342"
     CDS             24863..25687
                     /locus_tag="LR726_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL6215457.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LR726_000343"
     CDS             25680..26027
                     /locus_tag="LR726_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL6215458.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LR726_000344"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LR726_000344"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL6215459.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LR726_000345"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LR726_000345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL6215460.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LR726_000346"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LR726_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL6215461.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LR726_000347"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LR726_000347"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL6215462.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LR726_000348"
     CDS             31657..32451
                     /locus_tag="LR726_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6215463.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LR726_000349"
     CDS             32441..34483
                     /locus_tag="LR726_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215464.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="LR726_000350"
     CDS             34764..34913
                     /locus_tag="LR726_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL6215465.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="LR726_000351"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="LR726_000351"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL6215466.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="LR726_000352"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="LR726_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL6215467.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="LR726_000353"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="LR726_000353"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL6215468.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="LR726_000354"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="LR726_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL6215469.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="LR726_000355"
     CDS             39330..40412
                     /locus_tag="LR726_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL6215470.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="LR726_000356"
     CDS             40412..41215
                     /locus_tag="LR726_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL6215471.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="LR726_000357"
     CDS             41285..42025
                     /locus_tag="LR726_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL6215472.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="LR726_000358"
     CDS             42036..43040
                     /locus_tag="LR726_000358"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL6215473.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="LR726_000359"
     CDS             complement(43085..44782)
                     /locus_tag="LR726_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215474.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVKDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14415 a         7826 c         9339 g        13267 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctacaattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaaaaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctttca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataaca
//
LOCUS       DAFGFZ010000008        43654 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000008 DAFGFZ010000000
VERSION     DAFGFZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.008"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(225..704)
                     /gene="rlmH"
                     /locus_tag="LR726_000360"
     CDS             complement(225..704)
                     /gene="rlmH"
                     /locus_tag="LR726_000360"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL6215475.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1132..2421
                     /locus_tag="LR726_000361"
     CDS             1132..2421
                     /locus_tag="LR726_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL6215476.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2460..3065)
                     /locus_tag="LR726_000362"
     CDS             complement(2460..3065)
                     /locus_tag="LR726_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL6215477.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3270..3734)
                     /locus_tag="LR726_000363"
     CDS             complement(3270..3734)
                     /locus_tag="LR726_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL6215478.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3756..4076)
                     /locus_tag="LR726_000364"
     CDS             complement(3756..4076)
                     /locus_tag="LR726_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL6215479.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4220..5299)
                     /gene="pepA"
                     /locus_tag="LR726_000365"
     CDS             complement(4220..5299)
                     /gene="pepA"
                     /locus_tag="LR726_000365"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL6215480.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5499..5849
                     /locus_tag="LR726_000366"
     CDS             5499..5849
                     /locus_tag="LR726_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL6215481.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6072..7019
                     /locus_tag="LR726_000367"
     CDS             6072..7019
                     /locus_tag="LR726_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL6215482.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7076..8494)
                     /gene="pepV"
                     /locus_tag="LR726_000368"
     CDS             complement(7076..8494)
                     /gene="pepV"
                     /locus_tag="LR726_000368"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL6215483.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8643..9483
                     /locus_tag="LR726_000369"
                     /pseudo
     CDS             8643..9483
                     /locus_tag="LR726_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
     gene            9487..9927
                     /locus_tag="LR726_000370"
     CDS             9487..9927
                     /locus_tag="LR726_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL6215484.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            9977..10171
                     /locus_tag="LR726_000371"
     CDS             9977..10171
                     /locus_tag="LR726_000371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215485.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10307..10993
                     /locus_tag="LR726_000372"
     CDS             10307..10993
                     /locus_tag="LR726_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6215486.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQPGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(11401..12090)
                     /locus_tag="LR726_000373"
     CDS             complement(11401..12090)
                     /locus_tag="LR726_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215487.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12065..14194)
                     /locus_tag="LR726_000374"
     CDS             complement(12065..14194)
                     /locus_tag="LR726_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL6215488.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14242..15579)
                     /locus_tag="LR726_000375"
     CDS             complement(14242..15579)
                     /locus_tag="LR726_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL6215489.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(15831..16190)
                     /gene="rplT"
                     /locus_tag="LR726_000376"
     CDS             complement(15831..16190)
                     /gene="rplT"
                     /locus_tag="LR726_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL6215490.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16243..16443)
                     /gene="rpmI"
                     /locus_tag="LR726_000377"
     CDS             complement(16243..16443)
                     /gene="rpmI"
                     /locus_tag="LR726_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL6215491.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16515..17039)
                     /gene="infC"
                     /locus_tag="LR726_000378"
     CDS             complement(16515..17039)
                     /gene="infC"
                     /locus_tag="LR726_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL6215492.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17083..17208)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17359..17991
                     /locus_tag="LR726_000379"
     CDS             17359..17991
                     /locus_tag="LR726_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL6215493.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(17993..18898)
                     /locus_tag="LR726_000380"
     CDS             complement(17993..18898)
                     /locus_tag="LR726_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL6215494.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(18983..19642)
                     /locus_tag="LR726_000381"
     CDS             complement(18983..19642)
                     /locus_tag="LR726_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL6215495.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            19958..20902
                     /gene="mvk"
                     /locus_tag="LR726_000382"
     CDS             19958..20902
                     /gene="mvk"
                     /locus_tag="LR726_000382"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL6215496.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            20895..21872
                     /gene="mvaD"
                     /locus_tag="LR726_000383"
     CDS             20895..21872
                     /gene="mvaD"
                     /locus_tag="LR726_000383"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL6215497.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNFKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            21885..22970
                     /locus_tag="LR726_000384"
     CDS             21885..22970
                     /locus_tag="LR726_000384"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL6215498.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            22967..24031
                     /locus_tag="LR726_000385"
     CDS             22967..24031
                     /locus_tag="LR726_000385"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL6215499.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24280..25083)
                     /locus_tag="LR726_000386"
     CDS             complement(24280..25083)
                     /locus_tag="LR726_000386"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL6215500.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25103..25945)
                     /gene="kduI"
                     /locus_tag="LR726_000387"
     CDS             complement(25103..25945)
                     /gene="kduI"
                     /locus_tag="LR726_000387"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBL6215501.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(25961..26968)
                     /locus_tag="LR726_000388"
     CDS             complement(25961..26968)
                     /locus_tag="LR726_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBL6215502.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(26975..27619)
                     /locus_tag="LR726_000389"
     CDS             complement(26975..27619)
                     /locus_tag="LR726_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL6215503.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(27719..28477)
                     /locus_tag="LR726_000390"
     CDS             complement(27719..28477)
                     /locus_tag="LR726_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBL6215504.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28604..29755)
                     /gene="fucO"
                     /locus_tag="LR726_000391"
     CDS             complement(28604..29755)
                     /gene="fucO"
                     /locus_tag="LR726_000391"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBL6215505.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(29837..30148)
                     /gene="rhaM"
                     /locus_tag="LR726_000392"
     CDS             complement(29837..30148)
                     /gene="rhaM"
                     /locus_tag="LR726_000392"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBL6215506.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30150..30995)
                     /gene="rhaD"
                     /locus_tag="LR726_000393"
     CDS             complement(30150..30995)
                     /gene="rhaD"
                     /locus_tag="LR726_000393"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBL6215507.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31009..32265)
                     /gene="rhaA"
                     /locus_tag="LR726_000394"
     CDS             complement(31009..32265)
                     /gene="rhaA"
                     /locus_tag="LR726_000394"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBL6215508.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32262..33722)
                     /gene="rhaB"
                     /locus_tag="LR726_000395"
     CDS             complement(32262..33722)
                     /gene="rhaB"
                     /locus_tag="LR726_000395"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBL6215509.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            33875..34744
                     /locus_tag="LR726_000396"
     CDS             33875..34744
                     /locus_tag="LR726_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBL6215510.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(34870..35769)
                     /locus_tag="LR726_000397"
     CDS             complement(34870..35769)
                     /locus_tag="LR726_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215511.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(35934..36758)
                     /locus_tag="LR726_000398"
     CDS             complement(35934..36758)
                     /locus_tag="LR726_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL6215512.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(36769..38064)
                     /locus_tag="LR726_000399"
     CDS             complement(36769..38064)
                     /locus_tag="LR726_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBL6215513.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38080..38559)
                     /locus_tag="LR726_000400"
     CDS             complement(38080..38559)
                     /locus_tag="LR726_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL6215514.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38565..39593)
                     /locus_tag="LR726_000401"
     CDS             complement(38565..39593)
                     /locus_tag="LR726_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBL6215515.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39610..40716)
                     /locus_tag="LR726_000402"
     CDS             complement(39610..40716)
                     /locus_tag="LR726_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL6215516.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(40945..43542)
                     /gene="adhE"
                     /locus_tag="LR726_000403"
                     /gene_synonym="adhC"
     CDS             complement(40945..43542)
                     /gene="adhE"
                     /locus_tag="LR726_000403"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL6215517.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        12529 a         9354 c         7969 g        13802 t
ORIGIN      
        1 aaaattataa gagagggagg taatggtctg aacaccaaga attgttccaa catcgaattg
       61 cttaactaat gagttcaaag cttaattatg tggctcttat atttaccgag cgtggattgc
      121 gatctcttta gcgaaacaaa catctttcat aaaaaaaaca gccatatccg ctttcttgga
      181 tatgactatt tactactact ttactatctt tcattcttct tgtctcactt atgataaggc
      241 gcccccgcat tgatccggaa ggcacggtag atttgctcga caaggatcag acgcatcaat
      301 tgatgtggat acgtcatttt tccaaaagag atctgagcat tgcttctttt catgacggct
      361 tcgctgagtc ctaaagagcc gccgataaca aaaacaaggt ggctttttcc ttggatacct
      421 aattggtcga tttgtttagc aaaagattca cttgtaggat ttttcccttc aattgctaag
      481 gcatagacgt attcttgttc tttgattttc gctaagatcc gttccccttc tttttctttt
      541 acttgtctca tttgcgcttc gcttaaattc tccggggctt tttcgtcagg aacttcgatc
      601 aattcgattt ttgcataggc attcagccgt ttgacatact cattgatacc ttggatcagg
      661 tatttttctt ttaattttcc tactgagata attttgatat tcatgttttc tctcctttgg
      721 tcttcatgct cactttgttt attataatca attttttcgt ctatatgttt attttggcta
      781 tttttgttaa aaagatttcc ccaacagtta tgcacaacat ccacaggttt atccacagaa
      841 aacagtgtta ttttacctta tttcatcaca ttcattaggc aaacatacgt tttataagaa
      901 tttatccact ttatacacag gctgtggata tgttttatcc cttatttttc caatcctatc
      961 cgcgtagttt tacacagtat ctgtggaaaa agaaaatcaa atattttcat ctgaaaacaa
     1021 tgatttttcc taaagttttt cataaaaagt tcacagtctc aaagtagact aaagtcaaca
     1081 aataagacta tactaacagt agataaatga atgtaattgg aggaggaaca catggataga
     1141 aaaaatgtga caccaaagat gaaaaaaagt aaaaataata gcctctggcg taagttaggt
     1201 ctggggcttg ttggagggat cgttggagga ctactgacag ctggcatttt ttatgcagcg
     1261 atgggcactg gcaacaatag ttctactgct acaagcggca atcagaattc tgccggtgaa
     1321 acagttgtgg aaaatgtcaa agtcaatgta gattctgata tcaccaaagc ggtagataaa
     1381 gttcaagacg ctgtcgtttc tgttatcaac ttacaaagtc aaaaccaatc aagcggtttt
     1441 ggcggactat tcgggcagca agaagaaagt agcagctcag atgatagtaa tttggaagct
     1501 tatagtgaag gaagcggcgt gatctacaaa aaagacggga atactgctta tgtagtcacg
     1561 aataatcacg tagtagatgg ccagcaaggt cttgaagtct tgatgaaaga cggtacgaag
     1621 gtaaaagctg aattagttgg aacagatgct tattcagatt tagcggtgtt gaagatcaat
     1681 tcagacaaag tcgaaacagt tgcctctttt ggtgactcaa gtgctctgaa ggttggtgag
     1741 cctgcgattg cgattggttc cccactagga tctgaatatg caaattctgt cacttcagga
     1801 atcatttctt ccttgaaccg acaagtgaca agtacgaatg aatcgaacga aacagtcaat
     1861 atcaatgcga tccaaacaga tgcagcaatc aaccctggaa attctggtgg tcctttagtt
     1921 aatatcgaag gacaagttat cggtatcaac tcaagtaaga tcgcaagtac ttctgaatct
     1981 acttctaatg taagcgtaga aggaatggga tttgctattc caagtaacga tgtagtgaat
     2041 atcatcaatc aactggaaaa agacggaaaa gtgacacgtc cagctctagg tatcacaatg
     2101 gtcgacttat ctgccgtttc tactcagcaa caagaacaaa tcttgaagat tccttcatct
     2161 gtaactaacg gtgtcatcgt gacaaatgtc caaacagcta caccggctga aaaagctgga
     2221 ttgaaacaat acgacgtcat tactaagatc gatgacaaag aagtttcttc tggtgtagaa
     2281 cttcaatctg ctttgtataa gaaaaaagtg ggagattctg tcaaaatcac ttactatgaa
     2341 ggctcgaaag aaaaaacaac tactgttgaa ttgacgatcg atcaatcggc attaaaacaa
     2401 aatcaaaata gtgaaaatta ataaaaaaga ggttgtgaca aagacttgtc acaacctctt
     2461 tattttggaa atgcttctcc gactacggca tcctcaggca atactaagat tccttttttt
     2521 gctggagcat ttggcaaatg caattcacga gcggaacaga tcattccata gctatctacc
     2581 cctcgcaaag ttcctggcca gatcatcatt ccatctggca tcatagctcc tggttttgca
     2641 acgacaactt tttgtccagc ttcgatattg ggtgcaccgc aaacgatttg caatactgta
     2701 ccattgtcta cttctgtctc agtaatcgat aaatggtcag aatcaggatg tgggacgcat
     2761 gtttttacgt acccgacaac gatcttcggt tcatgatcag cttcaagctg ttcttcgaat
     2821 cctgcttctt tgatcaatcg attcaatttc gctaattcct catctgttaa gcttacttgg
     2881 ccgttccctt cgattgttag atgatcggag atatggaaaa agttccaagc aacaacttga
     2941 ccttgttcat ctttgatccg agcaacattc catttgcgtt caacagtgtt ttctgttcct
     3001 ttatcgtcgg ctataataac catcaaggtg tctccgacgt gtgctttgtt ataagcaaaa
     3061 atcatgttct ttcctccatt atcctttgct gagatttatc ttaacaaaaa attgcattca
     3121 ctgcatgaaa tttttttcat gctttatttc cgaagaagcc tcttctggaa caccatttat
     3181 tcgtaacaag ggactgtgac aagacttttg tcacagtccc tttactgttt tagttaattg
     3241 attaaaaagg tcgtacaagt tccacttgct taattacttg gctgttcttc ttcgtcagaa
     3301 acgatcaaga catcacaagg tgaatgacgt atcacgtagc tggctacact tcctgtcata
     3361 aagcgttcga ctgcatttag acctgactgc ccaaccatga ttagatcgat ctcatatttt
     3421 tttggcaatt tgactgccat ggattcacga acagaaccat atgcaataat tccttctata
     3481 tttgtgtaat caacagattt cccatattct ttacattctt cgatcatttc cttggctgtt
     3541 tcagtttgct gatcgactag attttgatca ataggtgcat agcccataaa gttataaaat
     3601 tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc cacttctacc
     3661 gcttttttga aagcagcaag tgcttgttca ctaccgtcga tacctactaa aatattctta
     3721 tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttgttac actttctagg
     3781 aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc taattctttt
     3841 ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc ggctacatct
     3901 atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt ttcaatttca
     3961 ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa gaaaaataca
     4021 ctctttccag tttctacgta gcctgctaat tcttctaaac tatttgggat aatcattctt
     4081 tgtcctctac tttctgttta tgtatttttc aattaaatta tacacctttc tttcgaacta
     4141 acaaacattg atcctctttt tataaaaaaa atctggcaaa aagcaatctt ttgccagatt
     4201 ttatagtttc actatcgcat tattttccgt atacgatcgt atttactgtt gatttatcca
     4261 aacctttcag tgtttgaatc aacatttctc gtgcagcttc aaaatcgcga atactgaaca
     4321 tcgtttgatg cgtatggata taacgtccca ctacgccaat cactgtactt gggattcctt
     4381 cgttttgtgt atgagcagca cctgcatctg taccgccttt tgaaacaaag tattgataag
     4441 ggatattgtt tgtttcagcg atatccagca agtactcgcg caaacgagga agcatgatca
     4501 aacctggatc ttggatacgc atcaatgtac cttctcctaa atgtccgaat gtgccatttt
     4561 tcgtcactgt atcatctgct gcagagcagt ctactgcaaa gaacagatct ggcttgaatt
     4621 tattgacaga agctttagaa ccacgcaagc ctacttcttc ctggacattc gcgcctgcaa
     4681 tcaatgtatg acctaatgtt tcattttgca acgcttctaa agcttctaat accattgtac
     4741 agccatagcg gttgtcccat gatttactga taatattctt tccgttggct gttttgatcg
     4801 tttcagcgta aggaacgatt gtgtctcctg gaaggacacc gtaactcatt gcctcttctc
     4861 ttgattcaaa cccagcatcg aacaaaatat cggttacttc tactgatttt tgtccgcttg
     4921 ttcctcgcaa taaatgtggg ggaacggaag aagaaataca tggataattc ttaccagtag
     4981 aggttttaag tgtaaaacgc tgagcagaaa caacgtaagg attccagccg cctagaggta
     5041 cgactttaaa aagcccgttt tcttggattt gtgtcagcat gaatccgact tcatccatat
     5101 gtgctgcgac cattacacgg ggtgcatccg cttcttcatg atggcgcaga ccaaaaatgc
     5161 cgcccaaacc atctaactgt aactcatcta ccagcggttc catatgactt tccatataag
     5221 cgcgaacatc ttgttcaaaa ccgcttgttc cttgtaattc tgttaactct ttgattcgtt
     5281 gaaatgtttt ttcttccata tgctattcgt ctaaatttca gactaaactc ctctcagact
     5341 tatattgatc tgcggataaa atatgatttt ttctgcagac cgatcgtatt tattatagct
     5401 tattttttct ggtagcggtt atttatttca aagaaaatat gagagtgtgg taaaataagc
     5461 acaaggagta acaagtgaga agaaggaggt atgtccacat gtatgaagaa agcgaactaa
     5521 gctattttaa aggcgggtta gcagttggaa tcggtctagg attattgagc ggggtagctt
     5581 caacgatttg gtatcatcgc aaaaaagtat tgtcagcaga ccaagtactt gaaaatatca
     5641 agtcatcttt tctaaaagaa ggtgcaatcg aaggttcttg gatctctttt gaaaaggaac
     5701 ctattaggaa attcgccgtt cggtcgagtg cttatcgggg aggtctttct cgaatagaag
     5761 atggcgaaac tgtttattat gaatttttag cagatgctca tacaggaacc gtattagaaa
     5821 tttcaagaaa aaaaggtgca acagattaat acgtcaaaaa agtgagagaa cgaatacgca
     5881 cccactattc aaaagagagg tatcatgtcc ttcttttgta gtgggtggtt ttttagatag
     5941 agctttccaa acatatattc cagtattttc tttttaaata aatgatattt agcgggaaaa
     6001 actagcattt cctttgatta tgaggtattt ttaatttagc ttttgttttc tatagttaag
     6061 gagagtgaaa aatggaacaa caagaaaaaa ttgctgttct gattccttgt tacaatgagg
     6121 aagcaacaat ttcaacagtc attgcagact ttaaacgaga attaccagaa gcagatattt
     6181 atgtctatga taataattcg accgatcgaa catacgaatt ggccgttcaa ggtggagcaa
     6241 tcgtaaaaaa agaacctcgt caaggaaaag gaaatgtgat ccgccaaatg ttctttgata
     6301 tcgatgccga ctattattta atggtagatg gcgatgatac ttatcctgca gaagctgtac
     6361 atggtttgct cgagaaatta cgttctggtg aagcagatat ggtcattggt gatcgcctat
     6421 caaatggaac ctactttgat gagaataaga gacctttcca cgattttgga aataaccttg
     6481 ttagaaatac aattactaga atgtataaaa caaaaattct tgatgtcatg accggctatc
     6541 gaggattcaa tcgtatcttt gtcaaaagtt ttcctattat gagttctggt tttcaaatcg
     6601 aaacggaatt gacgatccat gctttagata aaaaattcaa gttcgttgaa atcccaatcg
     6661 attatcgtga tcgtcctgaa ggcagcgaat caaaattgaa tacattctct gacggattca
     6721 aagtgatcat gatgattatt aaaatgttta aagattataa gcctttgctt ttctttggga
     6781 tttggaccat tttcttcttt ttatttggat tggtcacggg gattcctgtg attcgcgaat
     6841 atatgctgac aagctttatt acacgcatcc cttctgcgat tttaagtact ggattgatga
     6901 ctttagcact tctttcatta gtaacagggt tgattttgga tacagtcgtc acgaacgcta
     6961 agaaagaata tgaattgaat ctatatcatg tttatcatga ataccacagt agaaagtaaa
     7021 aaaaggatca agactgcaaa aataatttgc aattcttgat cctcttttta attaattatt
     7081 tcgccaaacg atagatggca tctgcataga ttgctgttgc tctgaacaag tcatcaagac
     7141 tcataaattc gtttgcttga tgcattgtat cagtatatcc tgggaacatc gctccataag
     7201 caacgccacg tttcaacaaa cgtccgtaag ttccgccacc gatgatttgt tcgtagcctt
     7261 tttctccagt gtggtcttca tagacttgca ataatgtagc caccaatgga tcatccattg
     7321 gtacgtaatg tggttccatt acgcgagcac ctcgagtaac tgttgcgcct tcttgcccca
     7381 ctgtttgttc gatgtcactt tgcaatccgt ctactgttac accttttgga aaacggaaat
     7441 tcatattgat gaagttcgct tcctctgtac cattttcttc aaaagcaaat agtccagcat
     7501 tcattgtcaa tttgcccatt ttttcatctt cataagcgac tcctagtttt tctccataaa
     7561 aatcttcatg gacataagca gctgctacat ggataaattg tttagcagat cctaggaatt
     7621 catagttatc taagaacaat gccaagaaag aacctgcatt gataccagat tgtgggcttg
     7681 caccatgtgc gcctttacca actaattcga tttttacata cgtattgtcc gcttcaatcg
     7741 ttccgcttac tggatttgct tcgatgaatt ggtaaaatgc ttcttccatc gcgatagctg
     7801 catctgcact tggtacttga agtgctgcag ttgctgttcc tggcaccata ttttcgcgaa
     7861 gtccggaaac aaaagatttc aagacataat ctcctgcatt gtcgcctttg aagcgtaaag
     7921 ctaaagaaac atttcctttt tcaccattga tgattgggaa ttcagcatct ggtgagaaac
     7981 caaaatctgg ttcttcttca tgttcgaaat aataagccat atcgccccag ccgctttctt
     8041 catcgctgcc gactacgaag cgaacttttt ttgatagttt caagtcaagt tctttaatga
     8101 tcttcatcgc atagtaagct gccatacttg gacctttgtc atcgcttgat ccgcgtgcaa
     8161 agatctttcc atccttgatt actggttcat aaggatctgt gtcccagcca tctcctgcag
     8221 gaacgacatc catgtgtcca aatatcccta gcgtttcgtc tccttcgcca agatcaatat
     8281 gccctgcata gttatcgaca ttttttactt taaatccatc acgttcgcca taagctaaca
     8341 tatgtttcaa tgcgtcgcga ggtccaggtc caaatggtgc atctggtgtt acttttgagt
     8401 catcgcgctc gctgttgaca cgtaaaagat ttttcaagtc ttcaagcaag tcttctttac
     8461 gtgcttctac ttctttttgc caatcaattg tcataaagtt aatcctccac ttatttaagt
     8521 cttcgtgttc atgataccac aaattacaga aaaaaatagt tcaaaaaatc tgacaattcc
     8581 ttttcttatc tgtttttctc taaaaaaaat atacgctata atgagtaaaa ggaggttttt
     8641 agatgctgat aaacgaacaa ctagtgaaaa gagtcatcac acctcgtcca aaaatttcac
     8701 ataaaggaac atttggacgt atcgttttga ttggcggcaa tgaacaatat ggcgatcatt
     8761 atgagtgctt tagctgccgt acacagcgga gcgggtctca cgactgtagt aactgatgaa
     8821 aaaaatcgga gtgctcttca cagtcatctg ccagaagcaa tgattgtagt ttggacggat
     8881 actgaccagt ttctttccgt aataaagcaa gcagatgtac tcttgattgg tcccggattg
     8941 ggtacttctt ctaagagttt ggaacgtttc aaacaagtac tagaaaacca atcaaaagaa
     9001 caatggctgg tgattgatgg ctctgcttta agtttacttg ctgctaatca tcttttgcta
     9061 ccctatcccg aacaaactgt acttacacct catcaaatgg agtggcaacg gttgagttct
     9121 ctaccgatac aagaacaacg ggataaagaa aacaaaaaga agcaacagga aatcggtgct
     9181 gtgctcgttt tgaaaagtca tgagacaaaa atttatggtc accagcactt ctacaccaat
     9241 cctttaggtt ctgctgctat ggcaacgggc ggcatgggtg acactttagc tgggatgatc
     9301 acaggatttt tagcccagtt tcctcaaaat gaccaaacga ttgcagcagc tatttatctt
     9361 catagtttca ttggagacca gctggcaaaa gaacaatata ttgttttgcc gacacaaatc
     9421 agcgaagcac ttccgaaatg gatgaattat ttttgtaaaa aatcaatgga aaacgaggca
     9481 tgagtcatgg agaaatttac aggttatgtg cgagaacctt tttattatga aacagatcaa
     9541 atggggatta ttcatcacag caattatatc cgctggtttg aagaggctcg ggtagcctta
     9601 ttggtacatc tagggtttcc ctataaaaaa atcgaagaat ctgggatcat catccccgtt
     9661 ctggaagtaa attgcagcta taaagaaatg atccattacg gagaaaaagt gagcatccat
     9721 ccaactatcc aaaagtatac ggggacacgg ttggatttta gctacgaaat tgtgggaact
     9781 gaagacaaaa aattaaaaac tactggcagt agtaaacact gttttttact ttctgattcg
     9841 caacgattag tgaagctgag caaagtcaat ccggagttag atcagttatt ccaagcgtac
     9901 gcttctttgg atgatgtaaa ttcttaataa agaccgtcta agcatttttc ttgtattgtg
     9961 tcgagtagtt cgtataatga aaagaaaaaa gcaggtgatc cccatgtatg tcgttaaagt
    10021 aatgcacggt tacatcgata aaactggctg tcgcactcgg gaaaaaaact tagacaatct
    10081 gctcattttc aaagacaaaa aagaatccga agcttttgct aaaagaatcg gcggtcgtgt
    10141 caaaccgatc caagaggtac gaccagattg attcaatatt tttcttttcg aggctctaat
    10201 aggcatgcct atcagggctt tttttgcaga ctgtgttttt atgcccacac gatttgtaag
    10261 cgatttatgt tataatagat tatatctgca tagaaaggaa ggttaaatgg ttatcaataa
    10321 tttgcaacag caagcttatg aatctatacg taaacaaatc atttattccg agttagcacc
    10381 tggaagtaaa atctccgata aagtgttaga agaacaacta gctatcgggc gtacaccgat
    10441 tcgagaagca ctgatccaac tgcgcaatca agagcttgta aaaaccattc cacagcccgg
    10501 cacttatgtt tcaaaaatcg atatccgatc tgcttcatta gcccgttata cgcgggaaaa
    10561 gttggaaaaa ccgatcctgc aagaatgcag tgcgaaaatg accaagcaag atcaagctaa
    10621 actagaaaaa atactagaac aaaccgatca ggcagtaatc gctcaaaata aaaaagcttt
    10681 ttttgatttg gatactgatt ttcaccgaac ttgttatgaa attgctggaa aacgagaaat
    10741 ttgggattgg ctagaaagtt atagtacgca cttgaaccgt ttcagatggt tacgtttgac
    10801 gatttcagaa ttagactggg gtcgagtatt agatgaacat cagacaatgc ttcaatcaat
    10861 gattgatcat aactttgatg aagtcggatt tctttgcacg atgcatttac atatgattat
    10921 cgaggagcaa gaatacgtga ttcataatta ccccgattat tttgaagata ttcaggacag
    10981 acccatcaaa taagaggttt tggaatcagc gttttgacct gagtattaga gaaagaaacg
    11041 gcaaaatagt tcctcatatt ttgccgtttt ttttgagcta ataccgaggg tcagctgatt
    11101 gcacaccgtt tattgagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
    11161 aagtaaaaat agcttcttat gtttttaact tggctggact tattaccgca gatgcaagct
    11221 tttgaacacc gttttccaag aggtcgtgag cctgccgttc atctccgagt cacaaggaac
    11281 aatttcaaaa aacagcttct catgtttgtt gaatattttg acttgtgacc gaggagttga
    11341 caggagaaca tcgtttattc ggaaaaaaag gactgtgaca cgacttttgt cacagtctct
    11401 ttatttgtaa tctaaatcat actccgtaca tcttttttct atttttttta tttcttctaa
    11461 gatcccttgg ctacgaattt cattatatgg ccaaagtaag actgcctcta atacataagg
    11521 cgaaaaatcg ctttgattca tctcttttga aaaactcatc gctgctatct cgctcaatgc
    11581 acgaatgttt gattgatgtg tataaaaagg caaccgccac agcacaattt tttcgctttg
    11641 cgtataaatt cccttaacag aagcttcgat gtggtgaaat agttcatgtc caataatcaa
    11701 agcttgcgct gtttctttag agatcgaagc tagtcctgaa cattttaatt tttctaacgg
    11761 ctccgtcatc aatcgaatcc cttcatcttc tgcataagtt gcaaaagtga ttcgttcgtt
    11821 tatttgctct gattcacagg aaacgattgg aacagccatt tgttcacaaa tttctcctac
    11881 atcggcaaaa gggtactttt ttttgatgct acatgccgtt tcgataccac aatcgattga
    11941 ttgttgtata agaattttct gcttggacaa aggaatcttt ttccaaaata aatctcgccg
    12001 aaactggtag aatccccaaa tttcttcggg gatctgttgt aattcgttta gtaactcttc
    12061 taacttatgt ttgtttgata tttccggcaa cgacgatcac ttcttcctcc ggttccatag
    12121 tagatacttc cagatccatc aaaagctgta gttgttccag atccgtcgct gtaaaatcag
    12181 agactcttgc tcctaagcag agataaaatt ctggtcgggc aatcaattca gtttgataaa
    12241 ctgccatatc ttcccaaagt tgttcgacag ctgtcatata attggctgca gttgttttca
    12301 tacatgaagc atgtccacgt tggaccttga taaatccttt ttgatcgatg atccgaagac
    12361 ttccagcatt ttttgaacga ttctgttcgc cgcatacata aaagaaaggt gtattttcca
    12421 ataagcaaac ttcattttta tttaatcgca tatcctctgc agcaagctct agtgcttctt
    12481 ctgttgtgat ctccttggtt agatcggttg ctttcacttc cgtagaacca gtagcgatag
    12541 ccgttacttt tgaagtctgt ggatcgattt ctacgtggac ttcgatcgat tctggtgtag
    12601 cgcctgattc aatcgcttta ttcatcgctt catttttcaa cgatcgtata tcttctttac
    12661 ttggcgaagg gatgatacgt tctacaacat ctcggaccat tgcaagtgct acaccaatag
    12721 aagaaatcac ttcggcgttt tcaggaatcg aatatttcac acccatttta ttactaaagt
    12781 aagtaatcaa tgaggcagcg ccgcccccta cacctacaag agaaatctga tctttctcca
    12841 agtgatactt gtctgccaat tctaaaatca ccggttcaat cttagcatag gatttttcca
    12901 tgatctgttc tgcgatgtct tcaaccgtcg tatgacaata atcagctaat gcttggatgg
    12961 cttttcgtgc agatgggaca tttccataag aaaaatgttc ttcttgcact aatccaagga
    13021 cattggctgc acatgtattt gtaatagtca cttcttctcc gtcttccatc actaccttga
    13081 cgtaatcagc agggtctcct tctttaggag agaaaaattc aaccttagga cctttgattt
    13141 tttctgtctc cgtaaatact gcataatcta gaccggcaat atgtgcagaa cgtggaccga
    13201 catcgatgat tcctgactga ttagcccgta ccatcgaacc gcctgccaca cctagaaccc
    13261 gaacatctaa tgaagagata tatgttgggt ggccacctac aattgaataa tcgattgctg
    13321 gacgtccatt tttaatgacc ccgatattgg ttgttgttcc gcctacttca aaataaacac
    13381 cattagacgc tctcaagtac atcaaagagc ccatgactga tgctgctgga ccggaaagca
    13441 tggttaaaac aggtcgtttt ttcatttcat tgatttccat tactccgccg tctccgcgca
    13501 taatcatcaa agaaacattg acccctgctt ctcgtacgga atcttctgta gaggttgccg
    13561 tatctagcat ttttggtaaa atagatgcat tgattgctgc tgtccgagtt cttcttgtca
    13621 atccatataa ttttgtgatg tcagaagcca tcgttgtagg gatcgatttt accgatgctg
    13681 cttcataaac aactcgttct ggttccccat tatctactcc aaatgccata gaagaaacca
    13741 agacctccgc tctttctctt tctaaagaag aaatcgtttc tgaaacacgg tctacattta
    13801 agtgtttgac aggtaagaat gcattgacaa tctctatttt tttcttattc cctaaatcga
    13861 tatcgttcaa tctagtttgt cgttttgcta aaaaaccttc taatccacct tttgccatcc
    13921 cgattacgcc gacttttgct acgtctcctt caatcagtgc attagtcgct tgtgtggtag
    13981 aatgtgcaac aaacacaaca tcttctggac tgatattatt ttccagcaaa caattttgaa
    14041 aagactgaac gacacctgcg gctactccac ggacatcatc gtgggttgtt ttcactgaag
    14101 attttccgat gatttcgtgc gtcgcattat cgattgcgac tgctttcgta tgtgtaccgc
    14161 caacatcaat tcccattcgc acaaaacgtt tcattttctt ttcctccatt catctaatcg
    14221 tttttatcga cgttttgttt actaggcgtt cccgtacaat aagaacgtga tcaggcataa
    14281 gatcgctccg atgatccaac cagttgggat ggatttcttc atatattctc gagaagttac
    14341 ttttgtataa cctaatcccc aagcaaccca agattgtgta atatctaaat gctgaggagc
    14401 aatcgttgtg attgcaaata acgggaacaa aaaggctggc gacatcgttg gattaacagc
    14461 taatacgaca gctaaaatag ctgagcctga accgactaga ttcattggac cgcggaaaaa
    14521 tcctagtggt gtcaatatag caaatacgat tgccaagact aatggtgatt gcggtatcca
    14581 atctccaaaa atagctgaaa aataaggaga agcatacgca gctgaattat tgaacatcgc
    14641 taaggtcatc aagaaaccaa ccattggcgc aacatctact gcgccatctg taaataattt
    14701 cgcaaactga cgacagattt ctttataacc tccgcgcaat tttccagtaa acagcaatgc
    14761 ccagatacct gctagaataa aacctaagat gattggaaga tctaaaagaa cgacccctaa
    14821 aactggtaat aatacggcta accatgagat cattggtgcg tcgccacctt cagctggtac
    14881 gtttgctgcc attgcatagc gtttcttttt gttcattgat atattagcga ccgttagtac
    14941 cacaactaag cttacaatca tcccaatcat accaatttgg aaatagtcat tataagtata
    15001 gctttcagcc gctggattga aaccagcata aatcgtttga tattgtttga aattgacgat
    15061 attagcaaag attccagcca taatcgagcc cataaatgag aacagtgcaa ccggtactga
    15121 aatacctaat gacatcaaga ttggcaacac aataactgca atagaaatca ctggtccgat
    15181 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
    15241 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
    15301 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
    15361 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
    15421 cgccgggttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
    15481 gatggcaaag ccgaccatca agttgtagcc ttttacacag taccagacca tgaaaaagaa
    15541 tgagataact aacaaaatac caataataat atcactcatg ttattcctcc gtccattttg
    15601 tttttctgat aagcaaacta atatatcagt caaactcatt ctaacttatg tagttagcgc
    15661 tttcattaaa gggtgaccat aaatgttcag ttttcaatta aaaaacctta attatgctat
    15721 tttagactta tacgttattg tcctgtttca tttgatggag gattgtaaat aaaaaaagag
    15781 gaaagaacat aacttttcgt tatcttcttt cctctttata attgtttagc ttatttagcc
    15841 aaagcttctt ttgcttggtc agccaatgca gtaaatgctg ctgcatcgtt aacagccaag
    15901 tcagctaaca ttttacggtt gatgtcgatt tcagcaactt tcaaaccgtg cattaatttt
    15961 gagtagctca agccattcat acgagctgct gcgttgatac gagcaatcca caatttacgg
    16021 aagtcgcgtt tcttttgacg acgatcgcga tatgcgtagt tgtatgaatt cattacttgt
    16081 tcttttgctg ttttaaataa agtgtgtttt gaaccgtagt aaccttttgc taattttagc
    16141 actcttttac gacgtttacg agttactgtt ccacctttaa cacgtgccat gtataattcc
    16201 tcctaataaa aataaatctt cagattctgt tatttgtact tcttatttca tttttgctag
    16261 ttgttggcga atacgtttga agtcgccact agaaaccatg cttgctttac gcaattgacg
    16321 gcgttgtttt tttgttttgc cgtggaaacg gtgacttgta aacgcgcgga agcgtttcaa
    16381 tccgccttta ccagtacgtt tgaaacgttt tgctgatccg cggtgtgttt tttgttttgg
    16441 catgactata tttcctcctc aaaatctttc gttatccgac tatcggctgt ccaacagcca
    16501 gttagtcatt caaattactt actgtcgttt ttcggtgcca gcgtcaagaa catgctgcgt
    16561 ccgtccattt tcgctttttg ttccactgtt gcaatatccg cagtttcttc agctaagcga
    16621 ttcaagactt tttgaccaat ttctttatgg gtaatggcac ggcctttgaa acggatagaa
    16681 gctttcactt tatcgccctt ttccaagaac ttacgtgcat tacgaagttt tgtattgaag
    16741 tcattcaagt cgatcgtagg acttaagcga acttctttta cattgatcac tttttgtttt
    16801 ttacgcgctt cgcggtcttt cttttgttgt tcgaaacggt atttaccgta gtccatgatt
    16861 cgcgcaactg gtggtttcgc accaggggca actaacacaa gatccaagtt tgcttgttct
    16921 gcaatattca atgcttctgc ttttgtctta actcctaatt gctcaccatc tgaaccgatc
    16981 aatcgtaact ctcgtgcacg aatgccgtcg ttaaccatca tatcctttgc tatggtcatt
    17041 cacctccaaa tattatgaga gaaagaaaag cttaccgaat aaacggcaat aaaaaaacga
    17101 gttctcttac agaacccgcg cgtacttcac ctaatagaac cagtgaaaac tatctagccc
    17161 agtgacatta tcaactaagg cgagaagcgg gtgcttctgc ttttatttct caaccatcat
    17221 agtatagcaa aaactaatct ttttgtcaat gtttttcata cagtttgtct tccttttctg
    17281 aaaatgatta gattagcaga ctttctagca gtgattcccg cttctttgct atactaaaga
    17341 aaaacgaggt gaacccacat gaaaaaaatc gaacctattc caccttctct tcctcttctt
    17401 acaaagggag agttttattt agaagatgag gagatcttct ctggtcttct gatcaacggg
    17461 ggtgaccaaa gctatctcga tgccaaaaat atggtattaa gagagagtca tatccaaaaa
    17521 cttacgatgc aaaaaacagt tttacgtcat tttgaatgca gtaatgtcat ttttgaaaaa
    17581 tgtgattttt ccaatctgga gtggattggt ggcagtttcc accaagtcgt gtttcatcaa
    17641 tgtaaattaa caggtacgaa ttttgccgaa agttatcttc gagactgtac tttcactgat
    17701 tgtattgctt cgatggcttc ctttagtagt acaaacttga aagctgtctc cttcgatcat
    17761 tgtcaattag aagacagcga attttatgag gtcacttgga aaaacctttt tttgtctgac
    17821 aatcagttga ccggttcgaa ctggtttcga acttctctga agggattaga ctttacgact
    17881 aatttcttca cagcaatcgc tttatctcaa gattctttgg cgggactaat cgtcaaccaa
    17941 gagcaagcac ttgtgatcgc ccaagccctt ggtttgcaga tcaaagacta gatcaacgaa
    18001 tccgccgcca cagtgcaata agcatccaaa tagataagac caaactgatg atcaaggcga
    18061 ttccccatcc gaaaacggaa tgggtaaagg gtaactggag attcattccg aagaatcctg
    18121 tcacaatagt tggaacggtc aagatcagcg accagaccgt caaaaatttc atcgtgtcat
    18181 tcaagttgtt attcagcaaa ttattataag tgccagagag ctggtctaaa atctgcgaag
    18241 ctaagttcgt catttcaact gcttgtcgtg cttcgatcag cgcatcgtcc aactcttctt
    18301 tttccttttc tgacaacttg cgataaatag ccaatgcctt gatttgttct aaaagtacta
    18361 cattttgttt tgttcctgta actaagtaaa ccaaaccgat ctctaaatct gaaagttgca
    18421 gcagattctt gttcgttgtt ttttctctta atttcagatt aagccgctga cgttcactat
    18481 tcacttcctc gattaaagga aaaaaggaat cagaaatcaa aaacaatgta cgaaacaata
    18541 aactgtatac actcatgtct ggcattcgtt ccaaaagcga tcgaattagt cgaatcacgt
    18601 actcggtatt cggcgtggta aatgtaaaca aaccgtcttc ttttaaaata aaagcgatag
    18661 gactagtttc ataatgatta tcgacttttt gctggtgcgg aacatttgat acaagcagta
    18721 acgcttcatc aaaagaatcg tactctacgc gcgcccgttc atttttatct agagaataag
    18781 tcagcatctc gtcactaatg ccatattctt cttttaagtt tttaacagct agcacatcgt
    18841 tttgctgtac attgatccaa aaattacggt cttgattaaa atagtgtttt tcccgcatac
    18901 tctatcctcc tgacatttgt ttccagtata aagaaaaaag tcgctcaaat aaaggtttac
    18961 cctttatttg agcaactgta aatcaaatgg ctactttggt ttggcgaatc tctgctaatt
    19021 tttcttcgat cattttcaag acttcttctt ttgcttgagg atcttccaca aaattatagc
    19081 ggtctccatc gatttggatt tttggacttt ctttgtattc ttcataccat tgatcgtaac
    19141 gtctgttcaa ttctttgtaa taatcgtata gacttggatc aaaggacaat tgttcatacg
    19201 aacgaccacg tttttcgata cgctccaaca tcgtatcaaa agatacacga atatgaacta
    19261 ataaatccgg atgtttttta tgggcagcat agggaagttc ttccatcatg ttttccagca
    19321 gttcatcata cacacgaact tctgtttctg ttgctcttcc tagatctgca tttagatgga
    19381 aaagcagtga atcttcataa atcgaacggt ccaatacatt attgtcatct ttcattgctt
    19441 gtttgatgct tgcaaaacgt ttattcaaaa aataaatttg cagcaaaaaa gcatactgtt
    19501 cagggtttgc ataaaataag ggcaagactt cattatcatc aatcgattca taaaatgcct
    19561 gactatcaaa gtgttcagcc atcatttctg ttaaacttga ttttccggca ccaattgtac
    19621 ctgctaaaac gatcacgctc atttacctcc agctcacttc atcactaaat attgtgttta
    19681 actccaacct ttttaaatat acccccaata tctagtagtg tcaattgtat ttttttctag
    19741 tcaaagaaga aaaaaagtca aaaaattctt aagaaaaaag tgttttttct ttagaaaacg
    19801 tattcttagt tttacttctg agatggtttt agtaaaataa aggaggaatg ttttttgaga
    19861 cccttagtgg acaaaaaaat tcgtgaagca gcgcaactcg ttttgggcgc tttatttttt
    19921 gcttaaaaat cacttttacg tgagaaggag ttagattatg gcaaactatg gccaaggaga
    19981 atcaagcgga aagatcatat tgatgggcga gcacgcggtt gtttatggag aaccagcgat
    20041 tgcctttcct ttctatgcaa caaaagtcac cgcattcctt gaagagctgg atgcaatgga
    20101 cgatcaactg gtttcttcct actattcagg aaatttagcc gaagctcctc atgcattaaa
    20161 aaatatcaaa aaattattca ttcacttaaa aaaacagcat gacatccaaa aaaacttgca
    20221 actgaccatt gaaagcacga ttcctgctga acgtggaatg ggatcaagcg ctgcagtcgc
    20281 cacagcagtc actcgtgctt tttatgatta cttagcattt cctttgtctc gtgaaatact
    20341 attagaaaat gtccagcttt cggaaaaaat cgcccatggt aatcctagtg gaatcgatgc
    20401 agccgctact agcagcttgc agccgattta ttttacaaaa gggcatcctt tcgactactt
    20461 ttctttgaac atcgatgctt ttttgattgt cgctgataca ggaatcaaag gacaaacaag
    20521 agaagccgtc aaagatgtcg ctcacctctt tgaaacccag cctcatgaaa ctggacaaat
    20581 gatccaaaaa ttaggatact tgacgaagca agcaaaacaa gcaatcattg aaaattcacc
    20641 agaaacgttg gcacagacaa tggatgaatc acaatcactt ctggaaaagc tgacaatcag
    20701 caatgatttt cttaatctat tgatccaaac agcaaaagat accggggcat tgggtgccaa
    20761 attaactggc ggtggacgcg gtggctgtat gattgcatta gcacaaacaa aaacaaaagc
    20821 ccaagaaatc agtcaagcac ttgaagatgc gggtgctgct gaaacttgga tacaaggatt
    20881 aggagtacat acctatgttt aaaggcaaag cacgcgcata taccaacatt gctctaatca
    20941 aatattgggg taagaaaaat gaagaactca tccttccaat gaacaacagc ctttcgttaa
    21001 cattggatgc attttataca gagacagaag tcatcttttc tgatagctat atggtagatg
    21061 aattttatct agatggcacc ttgcaagacg aaaaagcaac aaaaaaagtc agtcagtttc
    21121 ttgacctttt tcgtaaagaa gccggcctct cactaaaagc ttcagtaatc agtcagaatt
    21181 tcgttcctac cgcagctgga ttagcctcct ctgccagcgg gctagctgct ttagcaggcg
    21241 cttgcaatac tgctcttaag cttggattag atgatctctc tctttcaaga tttgctcgac
    21301 gcgggtctgg ttcagcttgc cgaagtattt tcggtggttt cgtcgaatgg gaaaaaggcc
    21361 atgacgactt aagttcttac gctaagccag tcccttccga ttctttcgaa gacgatttag
    21421 caatggtttt cgttttgatc aacgaccaga aaaaagaagt gtccagcaga aatgggatgc
    21481 gtcggacagt cgaaacatcc agtttttatc aaggctggtt agattccgtt gaaggggatc
    21541 tatatcaatt gaaacaagca atcaaaacaa aagatttcca acttctcgga gaaacgatgg
    21601 aaagaaacgg actaaaaatg catggaacga ctttagctgc ccagcctcca ttcacttact
    21661 ggtctcctga cagcttaaaa gcgatggatg cggtgcgaca attgagaaag caaggcattc
    21721 catgctactt tacgatggat gctggcccta atttcaaagt tttagtggaa aacagccatt
    21781 tatctgaggt acaagaaaca tttaccaaat tatttagcaa agaacaagta attactgcac
    21841 atgctggtcc aggaattgcg attattgaat aaagggagat ttttatgatt gaagtatctg
    21901 caccaggcaa actctatatt gccggagaat atgcagttgt tgaaacaggc catccagcag
    21961 ttatcgctgc agtcgatcaa ttcgtgacag tcactgtaga atctgcacga aaagtcggaa
    22021 gtatccaatc tgcacaatat agtgggatgc ctgtacgttg gacaagacgc aacggagaat
    22081 tagttttaga tattcgggaa aatccttttc attatatcct tgctgctatt cgcttgactg
    22141 aaaagtatgc acaagaaaaa aacattcttt tatcctttta tgatctgaaa gtaacaagtg
    22201 agttagacag ttcaaatggc cggaaatatg gattgggttc tagcggtgcc gtgaccgttg
    22261 caacggtcaa agccttgaat gttttttacg cgttgaattt atctcagttg gagattttca
    22321 agattgcagc actagccaat ttagcagttc aagataatgg ttcctgcggc gacatcgctg
    22381 ctagctgtta tggtggctgg atcgctttct caaccttcga ccatccttgg ctccaagaac
    22441 aagaaactca gcattctatc agtgagttac ttgccctgga ttggccaggt ctatccattg
    22501 agccattgat tgctcctgaa gatttacgtt tattgattgg ttggacgggt agccctgcct
    22561 ctacttctga tttggtcgat caagttcacc gttcgagaga agataaaatg gtggcttatc
    22621 agcttttctt aaaaaatagt acagaatgtg tcaacgaaat gatcaaaggg tttaaagaaa
    22681 ataatgtaac gttgatccaa cagatgattc gaaaaaaccg acaattactg catgatttat
    22741 ctgcaatcac tggggtcgtc atcgaaacgc ctgctttgaa caaattgtgt aatttagctg
    22801 aacagtatga aggagccgca aaatcttctg gtgcaggtgg gggcgattgc ggaatcgtaa
    22861 ttgttgacca gaaatctggc attcttcctt taatgagtgc atgggaaaaa gcagaaatca
    22921 ctccactgcc gttacatgtc tatagcgatc aaagaaagga aaaccgatga atcgaaaaga
    22981 cgaacatgtt tcactagcta aagcctttca tgataaacaa aaaaacgaat ttgacttcgt
    23041 acggatcatc cataaccctt tgccgcaaat agcagtttct gatgtcgatc taagtacaca
    23101 ggcagttggg tttacactaa gcagcccatt ttatatcaat gcgatgactg gcggaagcga
    23161 aaaaacaaaa aaaatcaacc aagatcttgc aatcgtcgca agagaagcag atttgatgat
    23221 tgccactggt tcagtcagcg ctgccttgaa agtcccttct ttagccgata cttatacgat
    23281 tatgcgacaa gaatatcctc acgggaaaat cattgccaac atcggtgcag ggacttctgt
    23341 cgaaagagcc aaagaagcta tccgattgtt tcacgcagat gccttacaga ttcatttgaa
    23401 tgcacctcaa gagttggtta tgcctgaggg agaccgtgat tttaccaatt ggaaagtact
    23461 tattcaagaa actcaaacag ccattgacgt ccccctcatc gtaaaagaag tcggttttgg
    23521 catgacaaga gaaacgctca atgatctagc tgctttagga gttcatacag ttgatatcag
    23581 cggtcgaagc ggaacaagct tcacccagat cgaaaacgcc cgtcgctcca agcgggaact
    23641 gagttattta gctgactggg ggcaatcgac agtctcttca ttgcttgaag caaatgaagc
    23701 agacacttct atggaaatcc tagcctccgg aggtatccgc aatgcctatg acatatttaa
    23761 agccctttgt ctcggcgcaa atgccgtggg tacttcagga accgtactga cgcatctgat
    23821 gaatcacggc gtagaagaaa ctattatact gatgaagcaa tggcaagaag agcttcgtct
    23881 gctttatact atggtcggcg caacgaatac cgcggcccta caccaacaat ctctgatctt
    23941 ttccggacca gtgaaggatt ggtgcgaagc aagagggatc gacttagtga agtatggaag
    24001 acgcacagaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    24061 gtacaaacgg cggaagtaaa agcaactcct tcggaaataa gctgaaattc acaaaaattt
    24121 gaaaagcaat tttcgtgaat tccttcttat ttcttggagt taaatgcttc tgtcccagcc
    24181 tcttttttcg aataaatgat gtttaatcag ctgtccttcg gtattcgttc aatccgctga
    24241 tttcacaacc tctccactaa acagtgtact aaagctcttt taaggttgtt ttccgatata
    24301 ggcaagtatg cctccgtcca catacaagac atggccgtta acgaaatcag atgcgctaga
    24361 agccaagaag acagatggtc cagccaaatc gattggctcg ccccagcgtg ctgctggcgt
    24421 acgtccaacg ataaattgat cgaatggatg acgttcaccg ttttcttgtg tttcgcgtaa
    24481 tggagcagtt tgcggtgtag caatataacc tggtccaatt ccattacatt ggatattgta
    24541 ttgaccgtat tcagaagcga tgtttttcgt cagcattttt aatccgcctt ttgccgcagc
    24601 ataagcactg accgtttcgc gtcctaattc actcatcatg gagcagatat tgatgatttt
    24661 accgccgcct ttttcgatca tatccggaat aactgctttt gaaacgatga atggggcatt
    24721 taaatccacg tcgattactt ggcggaagtc tgccgcatcc atttcgatca tcggtgtccg
    24781 tttgatgatc cctgcattgt tgactagaat atcaactgaa cctacatctt ctttgatctg
    24841 ttgaaccatc gcttgaaccg cttcttcatc ggtcacatcg cagacataac cttttgcatc
    24901 gatgcctgct tctttataat gcgctatacc ttcatcaact gattcttgat tcaagttatt
    24961 aaatacgatt tttgctcctg ctgtagctaa agagcgtgca atctcaaatc caataccata
    25021 gacagcaccg gtgactaatg ctacttttcc atctaaacga aacatatcca tactaaattc
    25081 catcattttc tctcccttat gcttatttta attgatccat ttgcaccata tccatgtctg
    25141 tataggtgat attttctccg cacattgccc agatgaatga gtaattgctt gtacctacac
    25201 ctgaatggat cgaccagctt ggcgaaataa tggcttgttc attgtctaca actaaatgtt
    25261 ttgtttcatc tggtttgccc atcatgtgga acacacgtgt atcttcattg gcataatcaa
    25321 agtagacata agcttccata cgacgttcat gtgtatgaca aggcatcgta ttccaagcgc
    25381 ttcctggttc taaaatcgta taacccattt gtaattgaca gctttcgcat acatttggat
    25441 ggatgtattg gtagatttta cgttcattca gtgtcaggcc ttcaccagtc tccattggtt
    25501 ttacttggtc gatactgatt tttacatttg gatatttatg atgagctgga acagaactaa
    25561 tatagaattt agctgggttt tcagcatctt gagaagaaaa tacaacatgt cgtgtttctt
    25621 ttccgatata gtatccgtct tgttttttca tttcttcttt ttgcccatcg atctcgatga
    25681 atcctggtcc accgatattg atcacaccta gttcacgacg ctctaagaag taatcaacac
    25741 ctagttcttt gtctaatgtg atttctaatt ctttttcagt aggtgtcact ccaccaaaga
    25801 tcattcgatc gttgtgcgta taagtcaaac tgattttccc tggttcaaat accttttcta
    25861 ctaaaaattc ttttctcaat tgttccgttg aataatgacg gatatcttct ggactgtgtg
    25921 tataacgtgt ttccatttct tgcattttct tctactcctt ctatcgaatg atttttccag
    25981 agccgcatgc taaaaattgt gcgacttctt cttgactaaa ttggttgcag tccccatgga
    26041 tcgtatgctt caaggcagaa gctgccgtag caaaatcaat gatttcttgc ggagacatat
    26101 tggataaaat cccatggagc acgccacctg caaaagcatc tccgccacct actcgatcca
    26161 cgattggggt gattctatgg acttgggatt ccacatattc atggttcatc cacaatgtcc
    26221 ccttcaattc attttcactt gctgaatgaa cggtacgctt ggtcgaataa aacacctgaa
    26281 tattcgggaa cttttcctgc attttttgat aataataaat tagaggttgt tcttctgctt
    26341 ctggcgcttt ctcgatacct aacaaataaa gagcatccat ttttccagct gagcagatat
    26401 ctactaaagg taaaagctga tggattactt cgctggcttc ttgctgtgtc cataattttc
    26461 cacgatagtt gatatcgaag ctgatcaggc agccagcttt ttttgcagct tctatcacct
    26521 gtttcgtcat tttttgccat tttttagaca aagctggggt gattcctgaa acatgaaata
    26581 tatctattcc ttggaataac tcctctagcg accattccaa atgatccatt tcagcaaaac
    26641 ttgatccggc acgatcatag atcactgagg cggcgcgttc gccactacca gtttccatat
    26701 aataagtacc tagtcttgat ccaccagata atacctgttt cgtatctact ccataacatt
    26761 gcaagtgctt tttgactgct tttcccaatg cgttatcagg tacttttgta gcaaaaatcg
    26821 cttcatgtcc gtaattagct aaagaaattg ctacgtttgc ttcccctccg ccataatggg
    26881 cagcgaatcg ttcacttgaa tcgatacggg ttccctgctc tgtggacaat cgcaacatga
    26941 tttctccgag tgtcactact tttcccataa gcagctacct cttgatttct ttcatttttg
    27001 ctgcatactg ttgcgcgact tccgttactt tgtcaaaatc tcctgtagct gcgggtgcta
    27061 aaaggttccc acctactccg actgttacga caccagcatc gaaccactct ttcatattgt
    27121 ccagactcac accgccagtt ggcatgatat tgacttgagg catcggcgcc ttgaaggcag
    27181 aaatgatact tggtccataa gcgcttccag gaaatagttt gacgatatct accccgcttt
    27241 tcagcgctgt tttgatctcg gtaatagtca tacatcctgg taagtaagga atttgataca
    27301 gattgcacat ctctgccgtt tcttgatcaa aggaagggct aacaatatac tctgcgccag
    27361 ctaagatagc caagcgagct gtgacagcgt ctaatactgt gcctgcgcca atcactattt
    27421 ctggttggtc tttgtagaat gacaaaagtt ctttgattac ttgatctgct tgagggactg
    27481 taaaagtcaa ctcaatacca gttagaccac ctttgatgat tgcgtggcaa gcattcaaag
    27541 cttcttcttt gctttttccg cgtaccaccg caatcactcc ggcgtttttc aatcgagata
    27601 aaatatcaat ttttttcatc ttctccacct atttcctatt tagaaatttt attttataac
    27661 ttggaaatct atacccatat tatacggcag aaaagaaaaa ttgcaaacgc ttttttcatt
    27721 atttttgaat tctttgctcg atttcgcttt ttgtttgctt gagggcactg attatttgtt
    27781 cttttgtctc atctgttaaa cgatatttcg gcatacttac actaaatgca ccaatgattt
    27841 gttcgttttt cacgattgag gctcctatac aaaaaatatc acgttcgatt tcttcatcgt
    27901 caaatgcgat gtgggtttct tttaccgttt tgatttcctt tttcaaccgt aatgcattgg
    27961 tgatcgtata ttctgtgtaa gggatcaacg gatgcttttc aagataatca gtgtactgct
    28021 cttcactgaa ttctgctaat acggctttgc ccatcgcaga attgtataat ggtcgggtga
    28081 ttccgatttt tgaagacatg cggatcgttt gatgttttgg atctagtttg ttcacgtaca
    28141 agatctcatt gttgtccaat acccctaagt ggatagtctc atcgatcttt tgctgcagct
    28201 gttctaaaaa cggtaaagtc aactctacca aatcgatttg ttcaatgttt ttattcgcgt
    28261 agcgaataag ctttgcacct aaacgatagt ttttctctga atttttattt acgtagccga
    28321 tcatcaacaa agtatctagt attttcaaga ctgtagaaga ggtcattccc acaccctttg
    28381 cgatcgtctg caaagagaca tcttggtttt ccgctaaata gtctagtatt ttagaggctt
    28441 tgatcaatac agttccataa ggctttttat tttccatata atattctcct atttctttat
    28501 aagaaaaatt atagcggaaa taccatcaat ttaaaagaga tatccagata taaactacca
    28561 tttctcttta caggcaattt aatgttgaag atccgcttcg tttttacatc aaactttgat
    28621 ataaagcaat gatttcttct actgtcgttt ctcttggatt tcctggtgtg catacatctc
    28681 gcaaagcatc ttctgcaatt gctggaatat ctgcttcttt tacacctaat tcagaaatgg
    28741 tttctgggat tccaaccgct ttgctcaacc gatcgatctc accacaagcc gcttctctta
    28801 catcttctaa agacatttca gctgctcctt taatccctag aacaagagcg atctcacggt
    28861 acttttctcc agtatattct ttgttatatt tcatgacagt tggcaaaagt gctgcacacg
    28921 ctacgccatg aggaatgttg taccatgcac ttaacgggtg agccatcccg tgtaccagtc
    28981 ctaaaccgac atttgagaag cccattcccg caatatactg tcctaatgcc attttttctc
    29041 gcccattttg atcgccatct acagaagctt ccaaagaatg gccgatgatt tcgattgctt
    29101 ttaaatgcag catatcagtc atttcccaag cgccttttgt gataaaccct tcaatagcat
    29161 gcgtcatagc atccatacct gttgatgcag ccaatttttt cggcattccc atcatcatat
    29221 cgctgtcgat gaacgcgacg attgggatat catgaggatc aacacaaaca aattttcggt
    29281 gatttacttc atctgtgatg acgtagttga tcgttacttc tgctgctgtt ccagaagtcg
    29341 ttggtatagc taatataggt aaacatggat ttttcgtatc agcaacacct tctaaactaa
    29401 tgacgtctga aaactctgga ttcgtcaaaa taatcccaat ggcttttgcc gtatcgattg
    29461 gcgaacctcc accgattgcg atcaaacagt ctgcctctgc ctctttaaca aaagccactc
    29521 catcttttac attttggatc gttggattag gtacgatccc gtcgtataaa gcataagcga
    29581 ttgctgcttt atctagaagt tctgttactt ttgttgctat accgtgctcg atcaatcctt
    29641 tgtcagtaat gacagctgct ttgccgaatc ctcgctgctg aaattcaggg acgatcgact
    29701 caatcgctcc ttttccaaaa tacgatgttt catttaaaat cattcgatta ctcattttct
    29761 attcctccta atttgtatta tttttcacaa taaaaacgaa agaaccccaa gcaaccggtt
    29821 gcccagttgc ttgttgctac agttcaaaga ctggcatcaa ttcccaactg actggagagg
    29881 catctggatt tgtctccatg atatcttcca tgtacttcca ccatttttga ttgatagatg
    29941 tatttgccat tttttcccat aacttttcat cttcgatcac taagtaacca aataacaaac
    30001 ttgtactcgg atcaagatag atcgaatagt cgatgacccc gtgttcacga agtgctgtac
    30061 gcatttctgg ccaaagttca tcatgtcttt ttttgtattc ttcttgtttt tctgggtaaa
    30121 ctttcatgca aaaagctttt ttcaccatgt cagacacctg ctttcgtatg aacttcctcc
    30181 aaataacctg cttttggagt gacaccaaac gcatctgcta aatgccacag gttttcgtcc
    30241 gtgattgttt gcaagattgg tccttgcgct ttgacgtacg tatagacttc tgctgccttt
    30301 tctgctgtct cgatcaaacc aaagacttca tccatgtctt ttcctgaccc gtagattcca
    30361 tgttgtggcc ataaaaccaa tcgagtttct ttcatttttt cagctgttgc ttccccaatc
    30421 tcatttgttc ccggaaccat ccacggaatg atggaaattc cttctggaaa aacaactaag
    30481 cattccgtac acatttgcca tagcgtacga ctaaaggaac gttcgtctaa ttcatgcgta
    30541 aaactcatcg ctaatagatg actagcatgg caatgcataa cgatccgatt atctgggtct
    30601 acttctaaac gggcgatatg actcatgaaa tgacttggca actcgcttgt aggaaggcat
    30661 tcgttttcca gcccccacaa aagttccagt gttttgccat cttcagcaac tcgaacgatt
    30721 ccaagattct cggcaggttc gctcgctaca tttttaaaat atcttcctga accagttaca
    30781 ataaaatagc atcctgctaa acggctggca tcaaaaatca tcgagatgcg gcggatcact
    30841 ttttcaggat ccaagaacgg tgtgatctct tcttctttta gtaaataaga aatattcccg
    30901 ccatttcttt catcccatcc caagcgataa agattcgacg tcgtttcgat catttctttc
    30961 acataaggcg cttgtaaaat atctattttt ttcaatatag ccacactcct attttcttaa
    31021 ttccaacacg tctttttgat attcctggac ttcttccagc caatccaaac caactggaac
    31081 ttgatttttg taacagaaat aattccatac atcggcatac gggaaatctt tcaattcttc
    31141 cgtataagcc attcttgaag taaagtcaaa agccagctcc atttctttca atcgctcaac
    31201 tggttccagc atagctttca gcagtgcttt ttgtgtattt ctcgtaccga tgacccaagc
    31261 agcgatacgg ttgatcgttg catcaaagaa atccagaccg atatttgttt tttccaacaa
    31321 ttcgttacgg actaattctt tagcgatttc ctgcaattca tcatccatca aaaccacatg
    31381 gtctgaatcc caacgcactg gacgggaaac atgcaacatg attcctttgc tgaataaagc
    31441 taatgaagat aatttattgg aaatcacttc tgttggatga aaatgtccag catctagaca
    31501 aacaagtttg tcacgagtca acccgtaacc catataaaat tcatgggaac cgactgtata
    31561 agcttcagcg cctaaaccaa atagtttgct ttctactgca tcttgtacat accgttcatc
    31621 taactcttct gtaaaaattt catcgagtga ttccattaaa cgttcccgtg gggtaaaacg
    31681 atcaatcgga ttatctttca tcccatcagg tacccaaaag ttattgatac ttgtttgccc
    31741 agtttctttc ccaaagtact ccgcgatttt gcggcttctt ttcccgtgtt cgatccagta
    31801 ttcacgaact tctttatctg gatgagccaa tgtaaagccg tctttcatca ttggatgcga
    31861 aaagaaagtc ggattgaagt caagaccgat ttcttctttt tttgcccact ctacccattt
    31921 ttcaaaatgt ttcggctcaa tttcatttag atcgacagat tcttctgtat ccaaatagat
    31981 agcatgaagg ttcaatttgt gtttgcccgg tatcaacgaa taagccttct ccagatcttg
    32041 tctaagctct tcaggtgttc gtgcggctcc tggaaaattc cctgtcgcca tgattccacc
    32101 ggtcaattcg ccatccggtg tcaagaaacc tttgacatca tctccttgcc agcaatgcat
    32161 agaaattttg atgtcctgta tcttttctag tacagcatct gtatctacac cgatagttgc
    32221 atatttttct tttgcttgtt cgtaatttct ttcaatcgtg ttcatacgat tactcctttg
    32281 attttattca cgtaccattc catcgtgttt tgcttatttt catctgggta aaaagtttca
    32341 catggaaacg actgtttgat tacatttctt gcttgttcta tattcgaaaa ctgacctgta
    32401 ctgagcattt gcatggcaac attcccgatt gctgtggctt cactaggtcc tgcttctaca
    32461 atgattccgg cataatctgc tgtcaattga ttcagtagac gattattcga tccgccccct
    32521 acgatgtgca gcttttccaa tttcgttcca gttaagtgtt ctactttgat cagctcttgt
    32581 gcgtaacaaa gagcaagatt atcatacaca caacgagcga tagctcccga tgagacaggg
    32641 actggctgct ctgtttcaaa acagtattgc tgaatctctt ctatcatatt tgggggattc
    32701 aaaaatcgag ggtcattgat ttccatcact tgctgcagcg gtttttcttt tgcagcaagc
    32761 tctgccaatt ccgcataact gtactcgtac tcttgcattc gggcaacttc ctggatcaac
    32821 cacatcccca tgatgttttt caaaaaacgc accgtgttat gggcacccca ttcattcgta
    32881 taattttcag aaaaagcatt gcgagaaaca ttggcgatct ttgtttcaat ccctaaaagc
    32941 gaccatgtcc cactgctgat ataaccccaa cgttctcctt gacctggcgt tcctaataca
    33001 gctgacgccg tatcatgact tgctactgtg ataaaggtca catctggcaa atcatatttt
    33061 gaaaattgtt ctttttgcag atctcctagt atcgttcccg gctctgtcaa tggtggaaat
    33121 aattccttcg agacaccgat tttttcaaga agttctattt cccattcttt tgtccctgca
    33181 ttcagcagct gcatagtaga tgcattagtt ttctccgtca ccatgttacc agtaaagaca
    33241 taccctaaat aatcaggtat cagtaacaat ttatgtgctt ttgccaaatt ttctttttct
    33301 tcagcaaaca attggaacaa ggtattgaaa ggctggatct gaatccctgt ctgctcatac
    33361 aacttatcta gagaatagat ttcagaaaac tgttctactg ctccattggt acgcttgtcc
    33421 cgataagcaa tcggatcatt tatccgatcc ccttgttcat caatcagaca gtaatcgact
    33481 gcccatgtat cgatccccgc aaaaaggcga tcaatccctg tctgctttaa tttttcaaat
    33541 ccaatcaaaa tctctttgat aagataatcg atcttccagc gatcgtgacc gtctttgttc
    33601 cgaaagccgt ttttgaaccg gtgtacttct tccaagatca aagaaccttt tgcatccctc
    33661 gtacttttca ttagtcgtcc gcttgatgca ccgatatcga tggcaacaaa tgttggattc
    33721 atttctccac ctcttttact ttattgttga ttagcacttt attaataaag cgttatcatt
    33781 tattgacaat gctatcataa tacagaatct tatattttgc ttcacactat tttggtatat
    33841 aattgcatta tcttgacaag ttgggagtgt ttacatggat tactgggaaa aagccaacca
    33901 ctcatggtcg gaagactcaa aaagagtgat cttgacgcct acacaaagaa gcaggaattt
    33961 atttttttat atccaagaaa tcggttactt caaagcacca atcccttact atactgaacg
    34021 agcacattta ccttcttact tgataaagtt tactttgtct ggcaaaggtt cgcttacata
    34081 taatgataat cagcaacagc taaaagctgg agacttattt tttattgact gccaagaata
    34141 tcagcgttat caaacgatca gcgaagaacc atgggaaatg gattggatcc atttgaatgg
    34201 tcagaacgtc caatcatttt atcaggagtt cacaaaagac ggccataaca ccttccattc
    34261 aacaggagat ccatcagaaa acaagatcca ccatttgatc acagaattga tccacatgca
    34321 acaaggcacg aatgcccgta ccgatttcca gtcctctgta gctatccacg agttgctgaa
    34381 tgaactgatt ttgcaaaagt atcagctaga tttcgaagaa gaggatattc ccgcctacgt
    34441 ttacaaactg aagcaatact tagacgaaca tttcaaagaa acgattacac ttgatcaatt
    34501 agaacaacaa ttcttattga ataaatatca gctgagcaaa gatttttcca aatttatcgg
    34561 ttctccacca atcgattatt tgatcagcaa aaaactttct tacgctaaag accttcttcg
    34621 gtatacagaa tttacggttc gaatgatttc cgaagaaata gggattgaga attttgctta
    34681 ctttagccgc ctgttcaaag caaaaacagg actatcgccg agagactttc gtaaattcag
    34741 ctgattcata taatatgtac gtcatttttt caaaatattt tgaaatatat agataaaaat
    34801 aaaataaccg cagtttctgc ttctatgtaa acaacagcag ctactgcggt tattttttag
    34861 tgatcattct cattgttcat cttcaaaatg ttcataatga accaactgtt tctgtgtatc
    34921 acgtttcaaa aattctttag gcgttatgcc gaactctctc ttgaatgctt tcagaaaatg
    34981 cggataagaa gaaaaaccac ttttactata tacagcacta gagctgcggt attctcgcaa
    35041 caagtgcttg gcatagatca gctttttctt cattacatac tgatgaaacg taaatccagt
    35101 gtacttttta aattctcgtg tgatatggta tttgctgaca aaaaactgct tttccatttc
    35161 ctctaaagaa agcggatgag ataaattttt tgagatatat tgtaacattt gttccacacg
    35221 ctcattagga actacagcat tctgttcttg cgcactctct tctttttgta caacttgatt
    35281 gagaaaaatc aagtattcta ccagagcttg ttcatatttt atgtcttcac cgtaaatttt
    35341 tttatcagga ggatggtcga taaatgataa tttttgcttc aatacttcct gttctatttg
    35401 caatacacga ctcctcttct ctcccattgg ttgaaaacaa gccgatagat tagaccatgg
    35461 cgtggaacga ctttctagaa aagaaggggt gacaaagata tagacacgct cgaatacatc
    35521 tgaagattgg cggacgatac gatgcaaatc attggaatgg atgagcagca cacttcctgc
    35581 tttgatcgta aattgtcgtc cgttcaaata aaaagtcgct tctcccttta atgtagcatg
    35641 gatctcatag aaatcatgat gatgatagat cgttttattt tggctgacga catctttgac
    35701 atgatagatt tcatactgat cagaaaccat cgtattgact tgttcaccgc tgattttctt
    35761 ttggagcatg tgccgcacct ccttttgttc acttatgcac aattttatta taccacgttt
    35821 tttgtaatca aaaagagctg cttttatata ctcttataaa aaagaagaga cgtggcatct
    35881 tctagccccg ccccctccat tctaaatatt agttaaccaa cgtcttcttt acattattca
    35941 agaagacttc ttccataacg aatcccatca tcttttgttt cttcaagaac cacatacagg
    36001 tacggacgat tcttcatgat gatttctttg atttttgtgt actcgatcac accgtctccc
    36061 aaatttaccg gcacgatttt ttcctgttcg acacgaaaat ctttcaaatg aaccgcacat
    36121 atcttttcac caaaacgttc aaaagcttct tctaccaatt ggacttgttc ttgataatca
    36181 ctacttgtga tcaaattggt cggatctaaa atgattccta gatagtccga atctactgct
    36241 tggatcagct gctctaccct tgctaaagaa tacaccggat gattcaaacc tggttctatc
    36301 cccaccatca tttggtattt ttctccagct tttaccaagc gacggataac ggacacagct
    36361 tcagcaaatg cttcatctgt gaaattttct tccgtatatt ggatttccgg caaaacacat
    36421 ccagtttccg aagcaaccat cgacgcacca aaggaagaag catagcgaag atactgctcg
    36481 aactttgtca gcaactcttg tcgtatcgtt aaatctggat gaatcatatt gatatagcaa
    36541 cttaaaattc ctacggtcac ttcttgcttt tctaaaactc gtttgacata atttcccatc
    36601 ccagaattga tctcatttgc gccagaaggt atttcaggaa acgaaagacc taaggcgagc
    36661 tgcagtgtat gaatcccttg ttcttttgtt ttttctgcaa gatcttctat cgtttggact
    36721 tcggaaagat catgtcctct aattgtaatg ttcaacataa ctattctcct aactcagcat
    36781 attgggtagc cacatgacga tttgcgggaa gtaagtaatc aaaaacaaga cgactaagat
    36841 aaccccataa aaaggaagaa gcggtttcag cattgcctct gcttttacgc ctcctacact
    36901 tgcaccgaca tatagtcctg tacctactgg cggcgtgatg gagcctacac acagattcat
    36961 gattgaaaac aacccgtaat gtacaggatc aacccctaca cttgtgacga ttggtaaaaa
    37021 tattggtgta aaaatcaaga tagcaggcgc cacatccata aacataccca ccagtaataa
    37081 cacaagattg actaataaca gaataataaa tttattatcc gttacgccca taatcaacga
    37141 actaattgcc tgaggaatac cggtaaacgc catggcgaaa gacatcatgg aagaagtagc
    37201 caataagaac atgatcgtcc ctgacattgc tactgattga aacaacatat taggtatatc
    37261 ttttagtttt accgttttat agtaaaacat agcaatcaac agggagtaaa tgacagctat
    37321 cgctgaagct tctactgctg taaatatccc cgtcaagata ccaccgataa tgatgacaat
    37381 caatagaagg ctaggaatcg cctcttttat tatttttcca gcatgagcca tctgtccttt
    37441 ttcgacaaca gggtatttat tctttttagc aacgatgaat gctactagca tgattcctag
    37501 cgcccacaaa gcgcctacga gataacctcc cataaacaaa gcagagatcg aagcaccacc
    37561 agcaacaagt gaatacatga taaaagcagt acttggagga atgaccatac ctgtcggagc
    37621 tgacgcgata ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca cttgctgagg
    37681 gatcaacact ccgccaatcg cggtagaagc ggcaatggcc gagctagaga tggaaccaaa
    37741 taaagcattc cctaacacat tcgtatgagc caaagccccc ggcacgcgcc cgacgaacaa
    37801 catcgcaaaa tccactagtt tcttagctat ccctccatta ttcatgataa tgcccgagag
    37861 aacgaaaaac ggaatagcga tcagcgaaaa actattgata cttcccacca ttttttgagc
    37921 tgaactaaaa gcagaaacat caaacggtat aactaacaat aaagtcatca tcgatgagaa
    37981 tgcaatactt atagcaatcg gcatccctaa tacaagcaac acaacaaata caaaaaataa
    38041 cactaatccg gcttctattg ccattttatt cctccatttt tatatttttt cttgaattgc
    38101 tggcttgttg cttaatgtat cgattagatt gatcaggcta tagataatga tgagtacccc
    38161 cgatacaggt actgacagat aaacatatcc cataggaata cttaaagaag gcgagatctg
    38221 ccccattgtc aatgtgacag cattccatcc gccatacacc atgatcatcg cagcaaatac
    38281 cagaaataaa ctttgaacaa caacttctaa aattttactt tttttgccag ttaatttttc
    38341 acttaaaacc gttacggcta aatgtgcttt ttgtcccaca acatacgcgg aagacaacat
    38401 ggcaaaccag acaagaccga atcgaacaag ctcttccgtg accgtatttg gatttcctaa
    38461 aaccgttctg gaaaatactt gccataaaac aacagcgacc atcccaacaa gcaaaacagc
    38521 tcccaggatc tctaatacct tgtttaaaat cattttcatc acagctacgc tccttgctct
    38581 tggatctttt gataaaggtc tttgtaggta ggatcgtttt tgaatatatc atgaagtggg
    38641 gcaactgcct ctcgaaacgg tgtattatct acatcattga attggactcc atgttcttct
    38701 tgtgctaaag ctttttcttc ttcaattgct tttgcaaaag cttcaatttc aaaaaccgtt
    38761 gattctttcg ctgcttcttc aatggctttt tcctgctcat cagttagctg ttctttgatg
    38821 gcatcattca tgatgatgat atctggcaca cgtgtatgct catcatagga atagtattta
    38881 gccacaccac catgtccagc tgtatacaag acaaattcat tgttttctga tccgttgatc
    38941 aaattggatt gaagagacgt gtacacttca tcacttccca taggtacggg cgaaccgcct
    39001 aaaagctcta ccattttgat agcggtttcg ctttgcatca cacggatctt ctttcccttc
    39061 aaatcttccg gcgtatggat cggaccgtct accatataaa agctgcgctg accagaatca
    39121 taataggtca atccggtaaa tcctaaattt ttggtcgatt ggtagattgg ctccattatt
    39181 tcttcattat ccatcacctt aaagaaatgt tcttcatcat tgaatagata aggaactgaa
    39241 aaaatcgaat aatctttaga gaaactttcc aacgctgagg cacttacttt tgtgaaatct
    39301 actgctcctg actgtgtcaa ttcgatcagt tcacgttctc cacccagttg tccatctggg
    39361 aaatactgga tctgtacgga accatctgtt ttttctttta ctaaacgccc aaattcattc
    39421 attgcatcaa ttactggctg gctgttgctt gcataagcaa agcgcaaggt aactatctca
    39481 tttacatctg catctgcgac agttgccaca ctagaattat ttccgcagcc gcttagacct
    39541 agcagaccta ggctaagcga acttgctgca aacagcagtg atgttttttt catatgggtt
    39601 tcctttcttt taacaataag agacgagcaa ttctgtcaaa caaagcaccg ttaacgactg
    39661 tccataaggc attgctgtga ttccgatatt ccgataaaaa tctaaactgt cccccattcc
    39721 tgtcccgatt gaaacgtttt gaacttctcc tttttcatcg atttgttcaa gcaatccttg
    39781 gatagcacga taagcgactt ctttatattc ttgtggtaaa taacgtttat ggaccgcttt
    39841 caaaataccg taagcaaatc ctgcagttgc agaagattct acgtaagaag aaggatcatc
    39901 caacaacgta tgccataaac cgctttcaga ctgataagtt tttaatgcac gcacttgtgc
    39961 ttcaagtgtt tcgatcaata atttgcgcaa tgcatcttct ttcgataatt ctaagatttc
    40021 aatgatctct ggaatagcaa tcgtcaacca gcagtttcca cgagcccaaa atgcttcggc
    40081 atagttatga tttccttcaa acgtccagcc gtgataccac aatcctgttc gtttatccat
    40141 caagtatttg atatggatca taaactggta acgagcttct tctaaatatt ctgggcgatt
    40201 caacagtttc ccgatttttg ccaaaggaag cactgtcatc atcaatgtat cgtcccacaa
    40261 ctgattctta ttttccggtc catatgtggc atgctgtaac ccgtcttcat ttgtccgagg
    40321 catgtcattc atgacccatt ctgcccattg ttctaaataa ggcaaatagc gtgaatcttt
    40381 cgtgtcttca tacaagtaag ccatagtcaa tagcggtgcc attgtattga cgtttttggg
    40441 cggagcgcct tcttccatac gatcctcaaa ccattcattg accacttgat aagcgcgagg
    40501 attttttgtc atctggtaat ttttataaat accatataga ccgacacctt gtggccaatt
    40561 ccacactgtc cagcttttgt catctacttt cagtccatca aagtccaaca aaaattcacc
    40621 attatcgtct ttgatcgttg tcaggttttc aacgagcagg tcaatttttt ctttcactaa
    40681 ttctttaggt acaaaaatct cttctttaat aatcatcttt acgccacctt tttaaacgct
    40741 ttcattttat atccttaata tagcagtaat accgtccact ataaatatcg ctttttgaac
    40801 aaaaactgtc taaaattgac ctaaatgtta aataacaaaa gtaactttca gttacttgga
    40861 aattaaaaaa agaacttgga gaaacaacgt tttccaagtt ctttttcatc tattttctta
    40921 tcttttttct acgctttctt ttgcttatcc gttgtaagca tcaatgatga tctgtttcaa
    40981 ttcagagatc aatggttctt tcggattagc tgtcgtacat tggtcttcgt aagctaattc
    41041 agccatacgg tctacggttg tatctagtgt ttcttgtgtg actccttgag cttttagact
    41101 catgttgatg ccgacagatt ttcctaattc ataaacagcc gttgctaaag cttcgactaa
    41161 ttcctctgtt gtttcgcctt tcagacctaa gaattttgcg atatctgcgt aatctgtatc
    41221 tgcacggaaa tagtcatatt tagggaacat cgcatgtttt tgtggatctt ttgcattata
    41281 gcggataata tgcggtaaaa gaatcgcatt tgtccgtcca tgagggatcc cgtattcccc
    41341 gccgatttta tgtgcgactg agtgacaaat acctaggaat gcgttagcaa acgccatacc
    41401 agccattgta gaagcgttgt gcattttttc acgtgattcc atatcaggtg tttttactga
    41461 tttttctaaa tagtcaaaga ctaacttgat tgcttgtagg ctcaatccac gtgtataatc
    41521 tgaagccatg actgaaacat aggattcgat tgcatgtgtt aacacatcca tcccggtatc
    41581 tgctgtgaca gatgctggaa ctgacatgac gaattgagga tcgacgattg cgacatctgg
    41641 cgtcaatgca taatctgcta acggatattt cacatgtgtt tcactgtcag tgatgaccgc
    41701 aaatggcgtt acttcagaac cagtccctga tgttgtcggg atacatacga attgtgtttt
    41761 ttctggtttg tcgatcttat atgtccgttt acggatatcc aagaattttt gttttgcacc
    41821 aaagaaagat gtgtctgggt gttcatagaa catccacatt cccttagctg cgtccattgc
    41881 agaaccgcct ccaagagcga ttactgtatc tggcttgaag tctgccatca gtttcgttcc
    41941 tgcgtatacg gtatttgtcg atgggttcgg ttcaacatct gagaagactt cgatcttcac
    42001 atcattttta cgtcgtgcca atacttcacg aactgtatcg cagtaaccga attggaccat
    42061 accagggtca caaacgatca tgacacgttc aacgttttcc attttctcta agtacaatag
    42121 agagttcttt tcaaagaaga tttttggcgg taatttgaac cattgcatgt tatttctccg
    42181 tttcgctaca gttttgacat tgattaagtt tacagcagag acgttacgag aaacagagtt
    42241 cttaccataa gaaccgcatc ccaaagtcaa tgacggaatc atttcgttat agatatcacc
    42301 gatgccgcct tctgcagatg gtgtattcac taaaatacga caagctttca ttcgaagacc
    42361 gaatttgaca tgcaactctt cgttttctga atggataact gctgtatgtc ctaatcctcc
    42421 aagattcaac ataccttcac acaaatcaaa cgcatggtcc gtgttgttag ctttgatcat
    42481 ggcaagaact ggagaaagtt tttctcttga tagcggataa tctgggccta cgccttccaa
    42541 ttcagcaatc agcattttcg ttccttttgg tacactgatt cctgccaatt ttgcgatttc
    42601 catcgcagaa tgtccaacga ttgatggatt gaccgcatat tttccttcgt tcatcactgc
    42661 atcttctaat ttttgcaatt catctggttt tacgatataa acttggtgtg cttggaattc
    42721 tgcttttact gctgcataga tttctttatc cacgatcaca gcttgttcag aagcacaaat
    42781 cattccatta tcaaatgttt tcgaaacgat caaatcgttc actgcacgtt taatttttgc
    42841 tgttttttca atataagctg gtacgttacc tggacctaca cctaaagctg gttttcctgt
    42901 agagtaagca gacttgacca ttccagctcc acctgtcgct aagacgatcg cgatacctgg
    42961 atggttcatt agcatagaag tcgcttcgat cgaaggatgt tcgatccatt ggatacaatt
    43021 ttcaggagct ccggcagcga tagcagcatc acgtaccact cgtgctgctt ctgcagaaga
    43081 tttttgggca cttggatgga aagcaaaaac gattggattt cttgttttca acgcgatcat
    43141 tgatttgaaa atcgttgtgg aagtcggatt cgttgttgga gttactccgc aaaccacacc
    43201 gactggttca gcaatttcaa tcaacccttt ttggacatct tcattgatca caccaactgt
    43261 tttatcatgt ttgatattat gccaaatata ttcggacgca tacatatttt tgattgcttt
    43321 atcttcgtaa acaccgcggc ctgtttcctc tacagccatc ttcgctaatg gcatatgttg
    43381 atccaaagct gccattgcca tttcatgcac gatatggtct actttttctt gatcaaagct
    43441 ttccatttct ttcaatgctg catttgcttt ttctgccaaa tcatcaatca ttgcttgtac
    43501 gtcaattgtc ttcgtttttt ctttttctat tactttagcc atcattatcc tcctaatggg
    43561 tcatggttat ttgttaaata attcacatgt atatcataca atatttattt ttctatgtaa
    43621 acactttttt gtgatttttt tcacagttat tttt
//
LOCUS       DAFGFZ010000009        43015 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000009 DAFGFZ010000000
VERSION     DAFGFZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.009"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(133..306)
                     /locus_tag="LR726_000404"
     CDS             complement(133..306)
                     /locus_tag="LR726_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6215518.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="LR726_000405"
                     /pseudo
     CDS             754..3116
                     /locus_tag="LR726_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="LR726_000406"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="LR726_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL6215519.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="LR726_000407"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="LR726_000407"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL6215520.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="LR726_000408"
     CDS             5704..6489
                     /locus_tag="LR726_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL6215521.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="LR726_000409"
     CDS             6502..7353
                     /locus_tag="LR726_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL6215522.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="LR726_000410"
     CDS             complement(7477..7929)
                     /locus_tag="LR726_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215523.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="LR726_000411"
     CDS             complement(8394..8762)
                     /locus_tag="LR726_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL6215524.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="LR726_000412"
     CDS             8912..9394
                     /locus_tag="LR726_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL6215525.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="LR726_000413"
     CDS             9431..10441
                     /locus_tag="LR726_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL6215526.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="LR726_000414"
     CDS             complement(10556..11269)
                     /locus_tag="LR726_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215527.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="LR726_000415"
     CDS             complement(11459..12049)
                     /locus_tag="LR726_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL6215528.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="LR726_000416"
     CDS             12533..12760
                     /locus_tag="LR726_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL6215529.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13941
                     /locus_tag="LR726_000417"
                     /pseudo
     CDS             12776..13941
                     /locus_tag="LR726_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            14175..15218
                     /gene="hrcA"
                     /locus_tag="LR726_000418"
     CDS             14175..15218
                     /gene="hrcA"
                     /locus_tag="LR726_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL6215530.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15312..15875
                     /gene="grpE"
                     /locus_tag="LR726_000419"
     CDS             15312..15875
                     /gene="grpE"
                     /locus_tag="LR726_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL6215531.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15926..17755
                     /gene="dnaK"
                     /locus_tag="LR726_000420"
     CDS             15926..17755
                     /gene="dnaK"
                     /locus_tag="LR726_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL6215532.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17906..19072
                     /gene="dnaJ"
                     /locus_tag="LR726_000421"
     CDS             17906..19072
                     /gene="dnaJ"
                     /locus_tag="LR726_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL6215533.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19154..19819)
                     /locus_tag="LR726_000422"
     CDS             complement(19154..19819)
                     /locus_tag="LR726_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215534.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19795..19968
                     /locus_tag="LR726_000423"
     CDS             19795..19968
                     /locus_tag="LR726_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215535.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20196..20402)
                     /locus_tag="LR726_000424"
     CDS             complement(20196..20402)
                     /locus_tag="LR726_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215536.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(20428..20847)
                     /locus_tag="LR726_000425"
     CDS             complement(20428..20847)
                     /locus_tag="LR726_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6215537.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            21351..21704
                     /locus_tag="LR726_000426"
     CDS             21351..21704
                     /locus_tag="LR726_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215538.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(21764..21931)
                     /locus_tag="LR726_000427"
     CDS             complement(21764..21931)
                     /locus_tag="LR726_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL6215539.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(22050..23969)
                     /gene="tet(M)"
                     /locus_tag="LR726_000428"
     CDS             complement(22050..23969)
                     /gene="tet(M)"
                     /locus_tag="LR726_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL6215540.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(23985..24101)
                     /locus_tag="LR726_000429"
     CDS             complement(23985..24101)
                     /locus_tag="LR726_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL6215541.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(24346..24423)
                     /locus_tag="LR726_000430"
                     /pseudo
     CDS             complement(24346..24423)
                     /locus_tag="LR726_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(24577..25212)
                     /locus_tag="LR726_000431"
     CDS             complement(24577..25212)
                     /locus_tag="LR726_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6215542.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            25724..26866
                     /locus_tag="LR726_000432"
     CDS             25724..26866
                     /locus_tag="LR726_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL6215543.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            26859..27065
                     /locus_tag="LR726_000433"
     CDS             26859..27065
                     /locus_tag="LR726_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBL6215544.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            27144..28337
                     /locus_tag="LR726_000434"
     CDS             27144..28337
                     /locus_tag="LR726_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6215545.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(28508..29245)
                     /locus_tag="LR726_000435"
     CDS             complement(28508..29245)
                     /locus_tag="LR726_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL6215546.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(29247..30749)
                     /locus_tag="LR726_000436"
     CDS             complement(29247..30749)
                     /locus_tag="LR726_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL6215547.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            31008..33002
                     /gene="uvrB"
                     /locus_tag="LR726_000437"
     CDS             31008..33002
                     /gene="uvrB"
                     /locus_tag="LR726_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL6215548.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            33012..35828
                     /gene="uvrA"
                     /locus_tag="LR726_000438"
     CDS             33012..35828
                     /gene="uvrA"
                     /locus_tag="LR726_000438"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL6215549.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            36091..37356
                     /locus_tag="LR726_000439"
     CDS             36091..37356
                     /locus_tag="LR726_000439"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL6215550.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            37360..38091
                     /locus_tag="LR726_000440"
     CDS             37360..38091
                     /locus_tag="LR726_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL6215551.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            38226..39110
                     /gene="rapZ"
                     /locus_tag="LR726_000441"
     CDS             38226..39110
                     /gene="rapZ"
                     /locus_tag="LR726_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL6215552.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            39107..40105
                     /locus_tag="LR726_000442"
     CDS             39107..40105
                     /locus_tag="LR726_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL6215553.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            40139..41074
                     /gene="whiA"
                     /locus_tag="LR726_000443"
     CDS             40139..41074
                     /gene="whiA"
                     /locus_tag="LR726_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL6215554.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            41617..42267
                     /locus_tag="LR726_000444"
     CDS             41617..42267
                     /locus_tag="LR726_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215555.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            42454..>43015
                     /locus_tag="LR726_000445"
     CDS             42454..>43015
                     /locus_tag="LR726_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL6215556.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        14199 a         7484 c         8810 g        12522 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
    13801 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
    13861 gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa tgtgtttgag
    13921 cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat ttaaataaaa
    13981 aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat ttacatcaat
    14041 tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag
    14101 gagaagggct tcttgctata tttataagtg gattagcact tagaatatat gagtgctaat
    14161 gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac
    14221 actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag ggagtcaagg
    14281 ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga
    14341 agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc
    14401 atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag
    14461 gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc
    14521 tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct
    14581 ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga
    14641 atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa
    14701 tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac
    14761 ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa gggatgtctc
    14821 agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc agtggacaaa
    14881 tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt
    14941 ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata
    15001 tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa
    15061 gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc
    15121 cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg
    15181 ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc
    15241 agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg
    15301 aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc
    15361 cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg
    15421 aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc
    15481 gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac
    15541 aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac
    15601 gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa
    15661 tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta
    15721 aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag
    15781 atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg
    15841 tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa acttacactt
    15901 tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac
    15961 taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg
    16021 aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt
    16081 tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg
    16141 tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc
    16201 aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa
    16261 agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc
    16321 tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct
    16381 tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg
    16441 cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac
    16501 agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt
    16561 agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg
    16621 cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat
    16681 cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac
    16741 acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg
    16801 tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta ttttagtcgg
    16861 tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc
    16921 aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt
    16981 gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat
    17041 cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag
    17101 taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt
    17161 acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga
    17221 tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa
    17281 cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat
    17341 caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag atgcagaagc
    17401 aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagatgc
    17461 attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga
    17521 agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag caaacaacat
    17581 cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa
    17641 actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag ctcaaggcgg
    17701 agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt
    17761 tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt
    17821 ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg
    17881 atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg
    17941 gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa
    18001 ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga
    18061 agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat acgggcatgc
    18121 aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg
    18181 cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg
    18241 cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga
    18301 cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga
    18361 aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa ctacttgtca
    18421 taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg gacgagtgat
    18481 gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag aaccttgtcc
    18541 aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc
    18601 tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg
    18661 cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata tctttgatcg
    18721 tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga
    18781 tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca
    18841 gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac
    18901 aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag
    18961 agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga
    19021 cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta
    19081 gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg ttcgcctggt
    19141 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata tgataatcgt
    19201 catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga tattgcaagg
    19261 tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta tttttactga
    19321 agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt ttcaaaatac
    19381 gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc ccgaggtcta
    19441 aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat tcgcaaactt
    19501 gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc gacccaattc
    19561 caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta tcaagcaatg
    19621 atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt ctatgtacag
    19681 tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat aaaggattga
    19741 tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca taatatggag
    19801 aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga tatcatcaat
    19861 gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga
    19921 aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa atacacggtc
    19981 ccttcatggc gaccgtgcat ttttggtttt tattaaattt tatatctaat tttcaattat
    20041 ccgcataaac actatacatt caagcatttt gaagtataga aaaacactca tcttaccacg
    20101 aatttaccac gattgaatga gccttgatat gaccgtaaaa ctactaaggg agataacacg
    20161 aaactgtgta tctcccgatt tttttataca gttgattact tcactccgat ttcaaatttc
    20221 aatattgctt tcatcatgtc tgtatgaatt tctcctttta gttcttcatc aacctgcata
    20281 atgattttac cactttcaga gtggtaaaaa ggacgcaagc ataatttaga aatataaccg
    20341 tcataaaact tcaaaatctc tttaactgcc atttcatctc cgcttgacgc tttgcaaatc
    20401 aattcaaaag agggatagcc ataatcttta cttcctttcg ctcaaaagca tttttaactt
    20461 ccttagtcct ctagttcttc tactgcaaat agcagaacgt gatacatggt acagttctga
    20521 aatttctctg tcgttcattc cttgaaaata acgtaataaa atcgcactac gctgtttttc
    20581 tgatagtcta tgtaaaactg taccaagtgt ttcatcagat acataaattg taaagtgcaa
    20641 gactttaaat gatacacaat cataaaaaca ggtatcaatc gttccacgtt caacttgttt
    20701 cgtttcggct atttcacaaa acaatacttc acgtttagaa tgtcttgcaa gctgtctgtt
    20761 tcgacttttc actttgttct taatgacaat cagcattaaa gaattgaact gtaatctgac
    20821 gatatgctcg aaagaagatg tcctcataat ctcaccccct ttctgtctag gaaaggaagt
    20881 gagccttgct cgtttatctc ctttcactct tagtcccaat gtgaaagggg gatttgttgc
    20941 attactgata aataaacttt gtaaaaaagt tctgaatagc caaaaaagca tataaacaga
    21001 tttatttctc tgtttacatg cttctgttat tctatctata tgatttataa aaccacattg
    21061 gtggacgtac ttatctattg cagatagacg actttttttg acaagaaccc aatgtaagga
    21121 aatttattgt atatgatgta cttcatggcg acgttgacct ccaacaaacc gccatttgga
    21181 agtaatatac aatattttaa cagcgtaaat agcactacca tataacggtt ttttttattg
    21241 gcgtttagta gtgcttttta ttaaatataa acctataaac catataacac gtttttctat
    21301 acctgttttt aattcagtag gaacaataaa atgtatagag gtggtctact atgcgtaaaa
    21361 aagaagataa atatgatttt agagcctttg gtttagccat taaagaagct cgattgaaac
    21421 gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat tgacccacgg tacttaacta
    21481 atattgaaaa taaagggcaa caccccagca tacaagttct ttatgacctt gtatcgttac
    21541 ttcatgtttc cgttgatgaa tttttcttac ctgctaataa cttggtaaaa agcacccgac
    21601 gattacagat agagaaatac atggatagct ttacagacaa agaactatcc ttaatggaat
    21661 ctttagccag cggtatcaac gaagcaagaa acatcgaaga ctaattaaaa gaatccatac
    21721 ataacggaaa gagccgataa aatgagattg tattaatctc attttatcgg ctctgcgtct
    21781 ttgcgtctgg ctctgtaatc acagttactt tgaactgctt tatttcaatt aaattttctt
    21841 gtctgcattt cggacaatag agggggaatt tttttaattc agtatcttcc cttatcttta
    21901 atcgtgtttt atttccacat acaggacaca atatccactt gtagtttata ataactatct
    21961 cctcctttac actttaattc aaatctttat taaaaaatat ttcatcttat ttaacaagaa
    22021 accatattta tataacaaca taaaatacac taagttattt tattgaacat atatcgtact
    22081 ttatctatcc gactatttgg acgacggggc tggcaaacag gttcaccggt agtaacatgg
    22141 taccctttta actctgttaa acaaacacta cgtccatttg taaagaaagt taaatcacta
    22201 cgatattctt gaatacaccg agcagggatt tctccactaa gaatgacctc attatttttc
    22261 aattgagtgt ctacgatgtt cgcacaatat ttaggagcat cgttgtatgc tcgtgaaaga
    22321 tattcctgtg gcgcataaat tttaaaacta agatatggct ctaacaattc tgttccagct
    22381 ttttttaaga cttgttccaa tacaatagga gcaagcatcc gaaaatctgc tggggtacta
    22441 acagggctat agtataagcc atacttaaaa cagattttac agtccgtcac attccaacca
    22501 tacaatcctt gttcacagcc atagcgtatc ccctccataa ctgcattttg aaacgattga
    22561 tttaagtatc caagagaaac cgagctctca tactgcattc cacttcccaa cggaagcggt
    22621 gatacagata aaccaatgga agcccagaaa ggatttggcg gcacttcgat gtgaatggta
    22681 tattctgcat tttttaacgg tctctccata taaatgactg taggctcttt tagttctatc
    22741 tccacatgat acttttcttg caacagtgca ctaatcactt ccatttgtac tttccctaag
    22801 aaagaaagta taatttcatg tgtcgtagaa tccacgtaat atcgtagaag cggatcacta
    22861 tctgagattt ccaaaagggc atcaagcaac atttctctct gttcaggttt actcggttca
    22921 acagttgttt gtagtagagg gtgcggattt tcaatctttt ttctctgtgg caatagtttt
    22981 gtatctccaa gaacactatt taacttcaaa aactcatttt gcaaaataac aatttctcca
    23041 gaataagctc tatcaatctt acataattca ccatttattg aagtatacat ttctgtaact
    23101 tttatttttt ctttttctga tactctaacc gaatctcgta aatgtagtac tccactataa
    23161 aggcgtatat atgcaagacg ttgtcttttt tttgtatatt caattttgaa aacatttccg
    23221 caaagttcag acggacctcg atgtgttgat gaataaaatt tattagtaat aacttctata
    23281 aggttatcaa tccctatatt actttttgca cttccatgat aaagagggaa cagagaacaa
    23341 ttctgaaatc ttatgctttc ctcttgttcg agttccaatg cttctaatga tttaccggac
    23401 atatatttct ctaaaaggtc atcgtttccc tctattaccg tatcccattg ttcagattcg
    23461 gtaaagttcg tcacacacat attaggatac agttctacct tctgtttgat tacaatttcg
    23521 gcagaaagtt tctctttaat atcctgataa accgttgata aatcaattcc attttggtca
    23581 atcttattga taaaaaagat tgtgggaatc cccattttcc taagtgcatg aaataatata
    23641 cgagtttgtg cttgtacgcc atcttttgca gaaatcagta gaattgcccc atctaaaact
    23701 gataatgaac gatatacttc tgctaagaaa tccatatgtc ctggcgtgtc tatgatgttc
    23761 accttcgtat tttcccactg aaaagaggtt attcctgtct gaattgtaat tcctctctga
    23821 cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt ccacgcttcc taattctgta
    23881 atcgctccac tgttatataa taagctttct gttaaggtag tttttcctgc atcaacatga
    23941 gctaaaactc caatattaat aattttcatg tgattttcct ccattcaaaa acccaaaagg
    24001 gcataaaaat cccagtgata aatactttta tcactgggat ttttatgcat aaccataggc
    24061 atacaaagca tacagatatt ctccggatac tttagaatca catgataaag gtattcttaa
    24121 actgggtaca aaaaactaag ccctcctaaa aaaggacatc caattatttg ttcccactat
    24181 caaattgaca gtttatttaa gaataccttg ccgcatattt attaactcct tttaaataga
    24241 tacttaaata atagcacgta agagcatatt tgtaaaggaa tctccaattt tttatcaaag
    24301 agagtacgtg attacaaaat agctgtaata atgtaccaat atttgttatt ctataatctt
    24361 ccaattactc ccgttctttt caagtaccaa atcaaattga gatacctgcg ttgcctttgc
    24421 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
    24481 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
    24541 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
    24601 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
    24661 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
    24721 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
    24781 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
    24841 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
    24901 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
    24961 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
    25021 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
    25081 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
    25141 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
    25201 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
    25261 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
    25321 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
    25381 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
    25441 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
    25501 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
    25561 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata caatcccaaa
    25621 gttgtgagta gtaatgagaa gttttaagaa gatagtgcat atcacacttt ttgaatagag
    25681 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
    25741 tgcacttgaa atgaagaaca aacgtcttgc aagcggtctt tcacaaggag aacttgcaag
    25801 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ccaacgccag
    25861 caaggaaaca caaaatatta tcctgcgttg tctgaattat tatatctgcg ataagccttt
    25921 ttatctgctc gttgattatc tgtctgtccg ttatccaacg actgacgcat tggaaattat
    25981 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
    26041 ctataaagag cattacgcct acggagaaat caaggtcatg gcttctgatg acgaacacat
    26101 gggcgtattc ttggaattaa aaggggcagg ctcacgcaac atggaatatg tcttgcaggc
    26161 acagaacaga gattggtatt catttttaaa ccgctgtctt gactgtgacg gtgttatccg
    26221 acgttttgac ttggcaatca atgatatgtg tggtctgttg gatattcccg ttttgtctga
    26281 aaaatacaga aatggcggtg cggattgtcg ctgtaaaaat tatgaaaatg tgcagggtgg
    26341 aaaactaagc ggaaaaaacc gtaatttagc aagcaccctt tatatcggtt caaaggcaag
    26401 cacaaaatac ttctgcctgt atgaaaaaca aaaggaacag gcaaccaaaa agaaacatac
    26461 ggatattatc aatcgttttg aaattcgtct gcgtggtaca aaagcagtac aggcagttga
    26521 ggaattgtta ttgacatata accctcatgg gctggtgttt taccttatta ctgattttgt
    26581 gcaatttccc gactatccgc tgtgggagat attcatttcc catgacagtt taccttttga
    26641 aatgaacccc gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    26701 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggctcaaact acatgaaaat
    26761 gattgatgaa tgtacccacc tctctgaaaa gcaggaaatg cttgtggaac agatgtgtac
    26821 ggacatagca gacgtaatcg aaagtgaggg ggtgttttat gagtaatgca caggatattc
    26881 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgtatcggag
    26941 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    27001 gcaaccgcat acagattaaa cgccgacagt ttgaacaggt cattgataaa ttggacgcta
    27061 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    27121 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    27181 tgcatactgg agagagccaa cgaacagaca gaaaatactt atataaatat gtagacgcat
    27241 ttggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    27301 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    27361 acggtatcga cagcactggc aagaaaatga cgctttgcca actttatgcc aaacagaacg
    27421 cacagagggc aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    27481 tgaaagagga caagttaggt gctaggagca ttgatacaat caaaccctct gacgctaagg
    27541 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    27601 gctcgttaaa agcgtcattc tacatagcca ttcaagatga ttatgtgagg aaaaatccct
    27661 ttgatttcaa gttgagtgaa gtcctagaaa atgataccaa aaagaaagtc gcattgacag
    27721 aggaacagga acaagcctta ctctcattca tcaagacgga caatgtgtat cacaagcatt
    27781 atgatgatgt gctgatactg ttaaagacag gacttcgtat ttcggaactg tgcggactga
    27841 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    27901 gcaaggaaca gggctattat attgaaacgc ctaaaacaaa aagcggaata aggcaagtac
    27961 cattaagcaa agaaacgata caggcatttc aacaggttat gaaaaaacgt ccaaaggcag
    28021 aaccatttgt gatagacgga cggggcaact tcctatttgt caatcataag ggcaagccca
    28081 aagttgcgat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    28141 acaaggacaa tccattgcca catatcacac cgcatacgct acgccataca ttctgcacaa
    28201 gactggcaag caagaacatg aacccaaaag atttacagta tatcatgggg cattcaaata
    28261 tcagtatcac aatgaactgg tatgctcatg cgtccataga taccgcaaaa tcagaggttc
    28321 agcgtctaat tgcatagaag tatttaccac gattttaacc acgcttgata gtgaaaatat
    28381 aagaagatag acctagatat gtgaggttta ccacaaaagc aaaatgcccg tagagcctat
    28441 aaaataaggc tttgcggaca tttaagaaga tataaaaaga tagtcaaaaa gacatatata
    28501 atttatttta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
    28561 tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga
    28621 cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac
    28681 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg
    28741 cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
    28801 ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta
    28861 gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct
    28921 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
    28981 agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
    29041 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
    29101 aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
    29161 cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
    29221 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
    29281 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
    29341 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
    29401 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
    29461 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
    29521 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
    29581 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
    29641 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
    29701 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
    29761 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
    29821 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
    29881 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
    29941 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
    30001 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
    30061 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
    30121 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
    30181 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
    30241 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
    30301 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
    30361 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
    30421 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
    30481 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
    30541 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
    30601 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
    30661 ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa gtcctgtcat taagaaaaca
    30721 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    30781 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    30841 cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg
    30901 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    30961 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    31021 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    31081 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    31141 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    31201 cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    31261 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    31321 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    31381 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    31441 catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt
    31501 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    31561 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    31621 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    31681 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    31741 acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    31801 tagcgaatat caaagaggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    31861 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    31921 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    31981 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    32041 cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    32101 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    32161 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    32221 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    32281 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    32341 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    32401 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    32461 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    32521 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    32581 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    32641 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    32701 tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag
    32761 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    32821 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg
    32881 aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt
    32941 tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    33001 aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga
    33061 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    33121 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    33181 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    33241 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    33301 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    33361 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    33421 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    33481 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    33541 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    33601 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    33661 ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg
    33721 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    33781 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    33841 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    33901 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    33961 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    34021 aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt
    34081 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    34141 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    34201 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    34261 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    34321 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    34381 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    34441 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    34501 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    34561 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    34621 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga
    34681 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    34741 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    34801 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    34861 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    34921 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    34981 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    35041 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    35101 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    35161 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    35221 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    35281 atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt
    35341 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct
    35401 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    35461 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    35521 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    35581 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    35641 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    35701 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    35761 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa
    35821 tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc
    35881 ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct acactgattg
    35941 gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg
    36001 actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa
    36061 aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt
    36121 atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa
    36181 gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt
    36241 aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg
    36301 atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct
    36361 tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt
    36421 atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat
    36481 gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg
    36541 ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct
    36601 gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta
    36661 gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt
    36721 gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg
    36781 tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat
    36841 cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc
    36901 tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat
    36961 atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt
    37021 cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat
    37081 cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca
    37141 agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac
    37201 aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa
    37261 aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca
    37321 agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt
    37381 ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg
    37441 aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct
    37501 gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat
    37561 gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca
    37621 cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga
    37681 gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca
    37741 gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca
    37801 gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag
    37861 gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc
    37921 ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct
    37981 gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa
    38041 cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg
    38101 atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa
    38161 actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg
    38221 taaatatgac agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa
    38281 cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc
    38341 gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt
    38401 taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa
    38461 tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac
    38521 tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca
    38581 ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga
    38641 tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca
    38701 aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt
    38761 tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc
    38821 cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg
    38881 atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat
    38941 acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc
    39001 gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg
    39061 tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac
    39121 ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat
    39181 cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga
    39241 tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag
    39301 aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg
    39361 tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc
    39421 agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca
    39481 tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa
    39541 agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg
    39601 tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc
    39661 agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct
    39721 tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt
    39781 ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca
    39841 tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac
    39901 ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt
    39961 cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt
    40021 agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg
    40081 tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat
    40141 gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc
    40201 gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca
    40261 gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt
    40321 gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa
    40381 gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga
    40441 tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca
    40501 aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga
    40561 aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg
    40621 cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat
    40681 ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa
    40741 ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg
    40801 tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat
    40861 cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca
    40921 ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat
    40981 gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga
    41041 atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag
    41101 caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac
    41161 tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac
    41221 aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt
    41281 gcttaatata gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa
    41341 ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca
    41401 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    41461 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    41521 taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact
    41581 tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    41641 atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    41701 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    41761 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    41821 acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa
    41881 atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt
    41941 agagggaatg tgacaactca acctagacga agttggagta taaaactttt aaataatagt
    42001 ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc
    42061 actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc
    42121 acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt agaagaagct
    42181 cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata
    42241 agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc
    42301 gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg aagcatggga
    42361 tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat
    42421 aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc
    42481 atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat
    42541 ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg
    42601 ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata
    42661 ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt
    42721 ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt
    42781 gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc
    42841 ataaagggtt atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat
    42901 ttatctagta ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg
    42961 ataattttta taatgcttat actgttttct gttttatttg gggtgatgtt tatag
//
LOCUS       DAFGFZ010000010        38263 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000010 DAFGFZ010000000
VERSION     DAFGFZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.010"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(188..1519)
                     /locus_tag="LR726_000446"
     CDS             complement(188..1519)
                     /locus_tag="LR726_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6215557.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1669..2382)
                     /locus_tag="LR726_000447"
     CDS             complement(1669..2382)
                     /locus_tag="LR726_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6215558.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2557..3981
                     /locus_tag="LR726_000448"
     CDS             2557..3981
                     /locus_tag="LR726_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL6215559.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4280..4987
                     /locus_tag="LR726_000449"
     CDS             4280..4987
                     /locus_tag="LR726_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL6215560.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4974..5297
                     /locus_tag="LR726_000450"
     CDS             4974..5297
                     /locus_tag="LR726_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL6215561.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5386..6423)
                     /locus_tag="LR726_000451"
     CDS             complement(5386..6423)
                     /locus_tag="LR726_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL6215562.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6744..7727)
                     /locus_tag="LR726_000452"
     CDS             complement(6744..7727)
                     /locus_tag="LR726_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL6215563.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8142..8912)
                     /locus_tag="LR726_000453"
     CDS             complement(8142..8912)
                     /locus_tag="LR726_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215564.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8905..9840)
                     /locus_tag="LR726_000454"
     CDS             complement(8905..9840)
                     /locus_tag="LR726_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6215565.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10156..11805)
                     /locus_tag="LR726_000455"
     CDS             complement(10156..11805)
                     /locus_tag="LR726_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL6215566.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11922..14525)
                     /locus_tag="LR726_000456"
     CDS             complement(11922..14525)
                     /locus_tag="LR726_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL6215567.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14537..15586)
                     /locus_tag="LR726_000457"
     CDS             complement(14537..15586)
                     /locus_tag="LR726_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBL6215568.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15612..15926)
                     /locus_tag="LR726_000458"
     CDS             complement(15612..15926)
                     /locus_tag="LR726_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBL6215569.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15942..16388)
                     /locus_tag="LR726_000459"
     CDS             complement(15942..16388)
                     /locus_tag="LR726_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6215570.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16389..18311)
                     /locus_tag="LR726_000460"
     CDS             complement(16389..18311)
                     /locus_tag="LR726_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBL6215571.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(18523..19419)
                     /locus_tag="LR726_000461"
     CDS             complement(18523..19419)
                     /locus_tag="LR726_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL6215572.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(19412..20092)
                     /locus_tag="LR726_000462"
     CDS             complement(19412..20092)
                     /locus_tag="LR726_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6215573.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(20093..20875)
                     /locus_tag="LR726_000463"
     CDS             complement(20093..20875)
                     /locus_tag="LR726_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215574.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(20868..21785)
                     /locus_tag="LR726_000464"
     CDS             complement(20868..21785)
                     /locus_tag="LR726_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6215575.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21949..22272)
                     /locus_tag="LR726_000465"
     CDS             complement(21949..22272)
                     /locus_tag="LR726_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215576.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            23060..26287
                     /gene="fss3"
                     /locus_tag="LR726_000466"
     CDS             23060..26287
                     /gene="fss3"
                     /locus_tag="LR726_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL6215577.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            26400..26714
                     /locus_tag="LR726_000467"
     CDS             26400..26714
                     /locus_tag="LR726_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL6215578.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            26727..27101
                     /locus_tag="LR726_000468"
     CDS             26727..27101
                     /locus_tag="LR726_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL6215579.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            27111..27446
                     /locus_tag="LR726_000469"
     CDS             27111..27446
                     /locus_tag="LR726_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL6215580.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            27529..28869
                     /locus_tag="LR726_000470"
     CDS             27529..28869
                     /locus_tag="LR726_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL6215581.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            28947..29621
                     /locus_tag="LR726_000471"
     CDS             28947..29621
                     /locus_tag="LR726_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL6215582.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            29614..29778
                     /locus_tag="LR726_000472"
     CDS             29614..29778
                     /locus_tag="LR726_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215583.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            29854..30996
                     /gene="dcm"
                     /locus_tag="LR726_000473"
                     /pseudo
     CDS             29854..30996
                     /gene="dcm"
                     /locus_tag="LR726_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            30986..31276
                     /locus_tag="LR726_000474"
     CDS             30986..31276
                     /locus_tag="LR726_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215584.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            31535..32719
                     /locus_tag="LR726_000475"
     CDS             31535..32719
                     /locus_tag="LR726_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL6215585.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            32716..32853
                     /locus_tag="LR726_000476"
     CDS             32716..32853
                     /locus_tag="LR726_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL6215586.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            33599..35509
                     /gene="ltrA"
                     /locus_tag="LR726_000477"
     CDS             33599..35509
                     /gene="ltrA"
                     /locus_tag="LR726_000477"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL6215587.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            35613..35837
                     /locus_tag="LR726_000478"
     CDS             35613..35837
                     /locus_tag="LR726_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215588.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            35850..36353
                     /locus_tag="LR726_000479"
     CDS             35850..36353
                     /locus_tag="LR726_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL6215589.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            36413..36802
                     /locus_tag="LR726_000480"
     CDS             36413..36802
                     /locus_tag="LR726_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL6215590.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            36789..>38263
                     /locus_tag="LR726_000481"
     CDS             36789..>38263
                     /locus_tag="LR726_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6215591.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT        12031 a         7208 c         7185 g        11839 t
ORIGIN      
        1 ttgcatatac taaatagacg tatatacaca gataggtttg tcttgtgaaa aataatgatt
       61 atccttacca aaaaatgttt gtttttctac atgttcgtac ttatcaaaaa atatgatcct
      121 tattgaaatt actggctatt tatattccaa cgacttcttg tgttaatttt tgttatctca
      181 tagtaaatta tgtttcattt tcacttaatt tagttgtcac gtgttcgtca attcgtaaaa
      241 acggtaaata tataaacaca cttgcggcta taattaatat ttgtaaaaca gctgctcgcc
      301 agtctccggc tgttgctaaa aatcccgata taactggtgg cgtagtccaa ggaatttgca
      361 ctactacatg attaattaat cccacagcag ttgcaaaata tgctactgtc aaagaaaaaa
      421 tcggtgcaag cacaaaggga ataatcagac tgatattaaa tacaataggc aatccaaaaa
      481 taataggttc gcttatattg aatatagcgg gagttaccga taatttagca aagtccttat
      541 aatcttttct acgactgaag ataaatattg caatcaataa agcaatcgta tttcccgatc
      601 cacccattac cgcaaaggta tctttaaagg ccatattgat aatatgtgga atttgctctt
      661 tgtttgcata agctgccata ttttcttgca tattctgaat caaaaacgga tctaacaatg
      721 aacctgatat tactgcttga tggataccaa atccaaaaaa tagattagca atagatgtaa
      781 ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa tggctctgta actatcgtgg
      841 taattagctt atttatatcc atattaaaca attgaatgat tataaatgac aataaagcaa
      901 acgaggtaat cgtaataatg attgggatta atacattaaa agactttaaa acagcgggag
      961 gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta ataaataatt
     1021 ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct gtataggata
     1081 taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta aacatcatct
     1141 gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca atatgatttg
     1201 tataatttct gcttgcacac atatgataag atactgctgc tacaactaat aatgtagtta
     1261 tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc aatgtactag
     1321 tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta ataagtgtca
     1381 caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc aaagttctta
     1441 aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat acaatgtcct
     1501 tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga ttactttttc
     1561 cacccaaata atacatcact tatcaaaaaa aagtaaatac ttttaattaa atatatatat
     1621 cttttttagt ataaaaaaat aagctctgaa aaaataattt tcccgagctt atttttcaac
     1681 aaaaacagct tcaaaaacaa agtcttgata taaatgtctc gataccgaat actcgaaagg
     1741 tttaccagac gatagagcaa caactttgtc tatctcaatc ataaattcac ctggatctat
     1801 ttttaatatt tttgcgtcaa attcattcgc agtatctcct ttgatacgta tgtgcgaagt
     1861 ctttatttcc agacccagtt catttgtgat ataagcatat acagatttct ttaaatgttt
     1921 tggttccaag cctggtatga ctgctaatgg catgaatgtt tgctcaatcg agtatggttg
     1981 acaatcaata aaacggcttc gtacaatatt atatacatag tcttttacac tgatacttaa
     2041 tttatcagct acttttttcg atggaagttc aattgagaaa gtatggattt ctgaatctac
     2101 gtgttcttca ccaaccacgc taaacaaacc ttcgtttgga ccaagaactc gtgagttatc
     2161 aattggtgtt ctgacgactt cagtcccata cccacttcta cttcgcaaaa ctccttcgga
     2221 aacaagtaag tctacgcctt tctttactgt taacttgctt gtatgataaa taactgccaa
     2281 ttcatctcct gtaggaagtt tctcaccaat tttatactct ccatttacaa tttttttctt
     2341 tatatctaag taaatttcca tatacttcgc aatttttttc aatggtgacc ctccaatcaa
     2401 aaacatatct tcatctatac tttaacataa ataaaccgat aaaatccaca tcaaaattat
     2461 acacacaata aaaaagatat atatatttaa acaaaactat ttactttaag ttgtaagcgg
     2521 tgttatcatt aagtggaaac aggaggtaaa aaaattatga caactatgaa aaagctaact
     2581 tttccagatg atttttggtg gggttccgct tggtcagctg agcaagcaga aggaaaagga
     2641 gaaacaggaa aagcagagac tatctgggat cgctggttta aagaacaacc ttaccggttt
     2701 tatcaacgaa tcgattcctc tattacaacc gatcacatcc atcaatataa agaagatata
     2761 gaactaatga aaaaaactgg gcataactcc tttcgcgttt ctatttcttg ggcacgaatg
     2821 tttccaaatg gcggtaccgg aaaagtaaat cccaaagcca tttctttcta tcgaaaatta
     2881 ttttccgaaa tgaataataa cggaatcaaa gtatttgcca acttatatca ttttgacatg
     2941 cctgctactc ttcaagataa aggaggatgg gaatcacgtg aagtagttga tgcttatgtt
     3001 cattacgctg aaacttgttt taaagaattt ggtgacttag tttgtcattg gtttaccttc
     3061 aatgaaccac ttgggcctat tttaggtact tatcttgaag attttcatta tccaaacctc
     3121 attgacttta aacgcggagc gcaagcagca ttcttcacta tacttgcaca cgcaaaagca
     3181 atacaagtat ttaaagaact agctcttcct agtaaaatag gcgttattct aaatttgagt
     3241 cctacttttc ctcgtagtca aaatcctgct gatgtgaaag cagcagaaat cgctgattta
     3301 ttttatacgc ggagcttttt agatcctatg gttaaaggga atttttctaa aaagcttatc
     3361 ctgttattac aagaatataa tcagcttcct gaagattata cgaaagaaga tcttgtatgt
     3421 atctacgaaa ataccgctca aattcttgga ctaaactatt acgagccacg tcgcgtcaaa
     3481 tctcgttctt ctatggtgga tacatctagt ccatttttac cagagtggtt tttcgaacca
     3541 catatcatgc ctggacgacg aatgaacgaa taccggggat gggaaattta cgaaaaagga
     3601 atttatgatt tgtgcatgga tatcaagaac cattatggaa acatcgaagc atttatctcg
     3661 gagaacggaa tgggcgttgc ggatgaagaa cgtttcatgg atgatacagg gcaaataatt
     3721 gatgattatc ggattgagta tatcaaagat catcttgctt acttatggaa ggcaataaat
     3781 gaaggatgta acattaaggg gtatcatctt tggacattta ttgattgctg gtcatggatc
     3841 aacgcctata aaaaccgata tgggctggtt tcactcgacc ttactactca aaaaagaaca
     3901 attaaaaaaa gtggcgaatt ttacaagcaa ttaagtgata caaatggatt tagatacgat
     3961 gtagacttgt tagttgaata atattctaaa attccacttg aagtgaacac attagaaaac
     4021 aagaataatg agaaatacca ataacaaata ctttttcttc atacttcatt tatacttgtc
     4081 agtataagag actgtgacaa atgtagtgat cctcagaagt taggtctgga aatctaactt
     4141 gagaagattt ttttaaacta cgtccagtct tttttatttg tttaaaattg aagttgacaa
     4201 gtcttcatta aaaatttaga atattcacaa gtttgacgac aattatgaaa atttatgtcg
     4261 tgctaaagga gttttgttat tggatgaaaa gttagatata aaaactgcga tcaaagacac
     4321 gttacctacc gtttttggtt atatcggtat tggacttgca tttggtatcg ttgggaaagc
     4381 tgccgggttt catccgctgg tcgtcacgtt gatgtccctg ctgatctatg ctggttctgc
     4441 ccaatttatc acggtcagca tgcttgctag ccatagtcca ctgctctcca tcgttctctc
     4501 gacctttcta gtcaattccc gaatgattct gatgagtatg acgattgctc cttatttcaa
     4561 gaaaaatcgg ctgcttcaaa atctgctgat tggcacattg ctgacagatg aaagtttcgc
     4621 actgggaatg aataaattga actatactgg acaaaagtta aactttcgtt ggatgaatac
     4681 agctaattgg atttcttatt tgacgtgggt gggttcttct cttgttggcg cacttttagg
     4741 gaattttatt actgatccga aaaagtttgg tttggatttc gccatcgtcg ctatgtttat
     4801 cgggttactt tatcttcaag tcatttctga tagaaacacc tctaaacgat tacagttgat
     4861 cctgattggt ctcactttga ttctggtata tgtcggactg atttttatcc caagtaactt
     4921 agtgatttta gttgtaacac tgattgggtg cggattgggg gtgtggatca aacatgcctt
     4981 cttttgagtt tacattgctg actattttag gttgtacttt agcaacttgg ctttctagga
     5041 tcctaccatt tgtcttactg aaaaaattcg atttgccgca accgttgctt gagtatttaa
     5101 gttttgtccc gatcgtcatt atgtctgctt tatggttcag cagtctattt acccaaaata
     5161 tcggacatct gccgcagatc aatctcgaaa atgctttggc ttcagttcct acacttttag
     5221 ctgctattct ttcaaaaagc ttattggtga tcgtcttggt aggaattctt tccctttcgt
     5281 tgattcgctt attataaata aaaaaacaag agcttatggc aaattactgt catgcgctct
     5341 tgtttttatc aaagtcaaat aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg
     5401 accactaaca acgacttttc ctaatatatg tcctgtttct acgtctttgg ttgcttgttt
     5461 caagttcttc gcattcatcc catctgaata gaccttgctc aatgtactgc ggataatccc
     5521 ttcatttgct aaagcagcaa tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt
     5581 atagtctgtt ttagcgaaca tatactccca gtcgaagctg atagattggt ttttccaaac
     5641 acctatatct aacggctctt tgatccctac gatcgttccc acatgtccaa atggtcggac
     5701 aaggtgtttg atttgatcaa agtagcgggt aatatcgaat aatacagcga catactctac
     5761 ttgatcgatt cctaattcac gaaaactttt acttaagtcg tgatgataat cgattggata
     5821 gtctacacct gtttttttca gccattcaaa attcttcgga cttgcagtcg cgtagacttc
     5881 taggccagcc caatgcgcta gttgattcaa aatcgaaccg acgcccccag ctccgttgat
     5941 cactaagatt tttttcccgc ggttggcatt ttcttctgga gtcagaccaa atttttcaaa
     6001 taacaattca taagcggtca atgaggtcaa cggcaaggct gcggcttctt catctgaaag
     6061 attttttggt gcaagagcga caatacgttc gtcaaccagt tggtattctt gattgcttcc
     6121 tgctcttgtc gttgtacctg cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc
     6181 atcacctaca gcaacaactt tcccgactcc atcaaaccct aagatacgta aagcatttct
     6241 tatcccgtta tcttggcgaa gtttcgtatc gacaggatta acagatactg cgttgacttt
     6301 taccagaaga tcccgtgaac tgggtacagg tatctgttct ttttcatcaa taaagctttt
     6361 tggatcatcg atcgacaacc cttcataaaa accgactacc ttcattaatt ccttttctga
     6421 catacgaatt cctccttagc ttcatcaagt tggagcatcc atacagtttt catttcaata
     6481 tatatagtat gctatttgtt ccacttttat tcatcataat tattctctta cttaaagtaa
     6541 actatttgca cttttttggt taaaaaagag gttgcgacaa gacttttgtc acaacctctt
     6601 ttaaacggtg ttcaatcaac tgaccctcgg tattcgctca aaaaacgaca aaatatgagg
     6661 agctattttt gtttggctgt tcttattact tgatgcagga cagctttttc acgacctcta
     6721 attcacggtg ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg ttctttttca
     6781 aaggtgtcta agatctctcg tacttcatca gtagatttcg tattcatcaa ctgaactcgc
     6841 aagtcatttg ctcctggaaa tcctttgata tagattttaa agaaacgatg tagtcctgtg
     6901 atggaacgcg ggatgatttc tgaataatga tcttgcagat ctagttgcag acgaagtagt
     6961 ccgatcagtt ctttttctgt atgttccctc ggttcttttt caaatgcata aggattcttg
     7021 aaaatccccc ggccaatcat gatgccatct acgccatatt tttcggccaa ttcttctcct
     7081 gtctggcgat ctgggatatc tccgttgatc gtgatcagtg tttgcggagc gatttggtca
     7141 cgtaaagcaa tgattttagg gatgacttcc caatgtgcat ctactttact catctcttta
     7201 cgcgtacgca aatggacaga aagattggct atatcttgat gaagtaggtg actgatccat
     7261 tcttccatct cagacatttc cgtatagccg atacgcgtct tgacgctgac aggcaaaccg
     7321 ccagctttag ccgcttcaat caattgtgcc gctacttctg ggcgaaggat caagccactg
     7381 ccttttccac gttctgctac attagggact gggcagccca tgttgatatc gattccttgg
     7441 aagcccattt ctgccatagc aatgctcatt tctctaaaaa attctggctt gtctccccaa
     7501 atatgggcaa ccatcggctg ttcatcttct gtaaaggtca atcttcctcg tacactgtct
     7561 tttccatctg gatgacagaa actgtctgag tttgtaaact cagtaaagaa gacatccggt
     7621 gctgctgcat gctttacgac atggcgaaat acaacatctg ttacatcttc cattggtgct
     7681 aaaacaaaaa aaggctttgg cagctctttc caaaaattag tagtcattta tttgttactc
     7741 tccattctat caagcagtga agtcgcaatc tttctcaaaa acattgtcat tatactagga
     7801 aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt ctgattgagc aaaaaaagac
     7861 tgtgacaaaa atcttatcac aattcctttt ccggaataaa tggtgttcaa aagcttgcat
     7921 ctgcggtaat aagtccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
     7981 tattacttga tgcagaacag ctttttcaca acctctccac taaacggtgt tcaatcagct
     8041 gaccctcggt attagctcaa caaacgacaa aatatgagga actattttcc cgtttgtttc
     8101 tctaatactc aggtcaaaac gctgatttca caacctcttt tctaacttct caatcctctt
     8161 cctttattga tcaaatacca agtaacggcg tacaaaacaa cgatgaatag cacaagtacg
     8221 atcatcgatg ttgctactgg cacatcgatt gttccaagga atccgtaacg gaaaccgaag
     8281 atcatataaa cgatcggatt gactttcgaa acagcttgcc aaaatggcgg caacatagag
     8341 atggcataga aaacgccacc tagataagtt aacggctgca agacaaatgt aggcacgatc
     8401 gatacatcgt cataagactg agcaaaaatc ccgttgagca gtcctgctaa cgaaaacaag
     8461 attgccgtca tcaataatgt aatgatgacc atgaaccatg aatacacatg aagtggtaca
     8521 aagaacaagg aaatgatcgt tacgagagag ccgacgagaa tactgcgacc cactccgcca
     8581 atcacaaagc cccaaatgat cacatgcgta ggaacaggcg ctaccaagat ttcttcgatg
     8641 tttttctgga atttttgaga gaagaaggac gaagaaacgt tggcatacga actagtgata
     8701 gcagacatca taatcaaacc aggcacgatg aattccatat atgaaaaacc acccatgtca
     8761 ccgatccgac caccgatcat ttttccgaaa ataacaaagt acaaggatgt cgtgatcaca
     8821 ggcggaacca gtgtttggac ccagatacgc aaatagcggt tcgtttcctt gatagctaaa
     8881 ctttttaatg cagtaaaata aagattaaac atttttctcc tccgtatgat tattttcttc
     8941 tgtgattttc aaaaacagtt cttctaaacg attcgactta ttcctcatag aaagaacttt
     9001 gattccttgt cttgtcagct gatcgaagat tccgttcacc ccttgatttc gttcaacttc
     9061 gacagcgatt gtctgttcat cttcaaatgt acaagcgtat ccttcaatca caggtttggt
     9121 ctcatatggc gctaaatcaa agataaatgt ttcaaattgc agtttggata gaagctcttt
     9181 catactagta ttctcgatca gttcacctga ttggatgatc ccgatgttgc gacaaagcat
     9241 ttcagcttct tctaaataat gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc
     9301 tcggagaaac tcccacatct cgcgtctcaa ttcaatatcg actcctgctg ttggttcatc
     9361 caagatcagc aaacgtggtt catgcatcaa tgccctagca atcatcagtc ttcgcttcat
     9421 tcccccggaa agcatacgtg cacggacatt tctcttttcc caaagattgg actgcttcaa
     9481 atatttttcg ctgcgtttca tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt
     9541 cacgacgatt tgctggaccg tttcaaaggg attgaaattg aactcttgcg ggactaatcc
     9601 aatctgttgc tttgctcgga ctagatccgt atccagatca taaccgaaga cattcacttt
     9661 tccagatgtt ttattgacta aagaagtaat gattccgatc gttgtagact tcccagctcc
     9721 atttggtcct aatagcgcat agaaatctcc ttctttcacg gaaagatcga ttcctcgcaa
     9781 tgcttcgacc ccacttccat aaaccttttt caaattattt atttcaagcg catttgccat
     9841 ttatttccta tctccttata gacatgattc tttattgtat catggaatac agcagatgaa
     9901 aaaacaactt ggttccagcg aaaaatcagg ttgctgggct caggaaaatc tcctaatgaa
     9961 agacatcaat cggacgaagt cagacaacct tttcagaaaa aacaaatcga agataaagga
    10021 ttactattca tatgtgcaac aaaaggagcg tgagacaaaa gtaaagaaca cttttgtctc
    10081 acgctctaaa cacgtataaa cggcggaagc aaaagccaag atttttgatc caataaatac
    10141 tttcgatcac ttcgcctatc ccaatacatg ccactgccgc ttttcatcaa ataacgcggc
    10201 ttccgttttt gcggtttcta ctaaagtctc tgggtaatcc aaaactttca gcaaggcaat
    10261 cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga ttccttttcc
    10321 ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa tctctgtcaa
    10381 ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc accgaacgac
    10441 tgaagcggaa gaagcaatcc gttcgatcgt atttgtccct tttaaaatct catctacgaa
    10501 gcagtagcag cgttctttgg aagccacttg gtccaacaaa cgtttgatcg atttgatttc
    10561 tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg aagtcaacac
    10621 atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct gtgctagaat
    10681 acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg aacccgtgac
    10741 taatgtattc ttctgccagt tgaccggatt cactaccgct tcttttaaca atggatgata
    10801 gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga tgggcatata
    10861 agtacggtaa ttcaatattg cagcggctac ttctaattct cccagcaatt cccacagctt
    10921 gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg agataaatgg
    10981 cagcatgaac atgatgttga catattcaaa aatcaactct gcttcactcc cactcttcat
    11041 acgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct gtttcagctc
    11101 ttcttgaagt ggtgtcggca gttttgctag ctgattggcg gctgaaatcg ttgaaaccaa
    11161 ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat aatagaccgt
    11221 attgaatact aacccaccga ttgccaaata gattcctagt acttgtccta aaagaagcaa
    11281 acccaaaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc gtccaatctt
    11341 tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt ttcctaaacg
    11401 agcgaattga tattggattt tttctctcag ttgaggattt tcctgataaa aagcaatcag
    11461 tttttctaac tgctgatctt caccaaaatc aaagctgcgt aaacgttgat acaacgcttc
    11521 tgaacctaca ctagaatagg tactgttgat tccttcgaat acttcgaaca tatccaggtc
    11581 ataccaagta agatcatcga tttcgctatc ccatgaacgg aacttcttct ccgtgagcca
    11641 tgcttccttc aagctttctt ctttatcaaa tctcggttga taaggaattt tttcccattt
    11701 gctacgaact gtttccttta atttcaaacg tgaaaaaatg tctaaagcaa ctaataaaat
    11761 aacagctgca ataataatta aaacgataaa ttctctttga cccatatccc tctccgttcc
    11821 tttatttgtt agctttagta tagcactgtt gcgctatttg aaaagagggt aaaaaaacac
    11881 ctttcaaaat aaaaggtgtt tttttcttgt atattacata tttatctctc atttatcaaa
    11941 attgacaaat aatcataggg aggaattgtt gttaattctt gtttattctc cagtgcattg
    12001 acaactgtcc caagggaaag gatagcctca ggtactattt tttcttgttc tgttggatta
    12061 gctaagcgaa gaacaaatcc ttgttcatga aaataagaag gataaatcga tgatacaatt
    12121 aagtcttcag gtaattcaat caatgaaaat ttacgaggaa catcaagttc attttgtaat
    12181 ggccaaatct tgttatctaa cctgtgaata aatacattca gtttctgctt ttgataagaa
    12241 atagattgtt ctaaccgttt ttctgcctgt tttgccactt tgaactcatt aaatcttcct
    12301 tcttctacat taaatccaaa agtgacttta tactcaccta tctcttgagc tagcggtgtt
    12361 tccatcatga catgcccttc atttgtcgta tctcctgaag ctcgcccagg tcgccaagca
    12421 agattaggtt ttccaagttc ccctgttgtt gaaaataagg ttatgattaa ttgattttct
    12481 atacgttcat actcttttaa accatctgca taaaacgtaa aataatgatc attttctgcc
    12541 acagagacac tcttatcgaa tatttctaaa ttaacaggtt tctctaccat agtttctgac
    12601 caattcggag gaatgacatc aggggtattt tcaataaatc cgttttgaat ttgtgcaatt
    12661 gatttttcag atttatctaa aacattaatt gctaagcgca tacgatgcga gtaaacagta
    12721 ttatgaattt tgagtgtgcc ttcaacgatt tgactttctc ccagactgaa cgatatttca
    12781 taagatattt cttccatttc gggtttttca gataaacgat cttttaaatc tttaggaagt
    12841 tgagcttttc cgtaaacaac taatgtttct tgtaaagaat ctttatatac tttagctgtt
    12901 tctaatttta gagatagttc tgtatctcct tctaatggtg aataatcata cgtatcgcca
    12961 tcatttgctg aatcgatcaa atgaacgaaa tcaagatatt gtctatctcc aactatcaaa
    13021 ttcaattgcc catctttaaa agaaagttta tatttattgt tacttatctc agttttgtct
    13081 atctcgattg ctgtttctaa tttttcatta tttttctcaa attcaacaac agtgtatcct
    13141 agtgctggca catttacatt tatttttatg tcaagttcat aataagctgg ttcatcaaaa
    13201 tattcgaaac ctgtagcaat catccgataa gcatttttcc gttctggaaa ataaacttct
    13261 ttttcaatga tattataggg actacctttg aagtgtattt tctttgaatc agctactaca
    13321 tgcacgattt tcgatccact ataattctca gtcgttgtgt tgaaaacaag gacttcattc
    13381 gattttagct ttaatgcatc agccatccgt tttacaataa ggttttcgat tccgtcagct
    13441 atctcattag cttctttgat tcgatggaaa atatcttccg caacattatc tgaaacacag
    13501 ccaccaatac tatcatgagc ctggttttgc aaaatctttt tccataaccg acgaagtagg
    13561 cctttactga tatggatacc atttctttct gcaatcagca tgagcggctc aactcgtttt
    13621 agaatttttt gttccaattt aaaattttct attttcattt ttgttcttac tgagccaata
    13681 gaacgatgaa cacgagagta aaccggctga cgaagttcac ctttatattc aggtaattct
    13741 tttttgtttc tgataacttc tacataagaa ggataatctg aaataatatt gtggaattta
    13801 ctgtatttat tcaacttctc tacgacttga tcaaaattta aaatcattga cttttgatct
    13861 acacccgaag gtactaaaat atctttgttt tttccttgca tatgaagcat ttcaactgct
    13921 tcatccaact tttcttttgc aaattcagga atatctttaa ttgcgtctaa ggtcatcatt
    13981 ccagtcatat aaccaaatgg aaaattcatg gcataaacaa atttatctcc caatcctttc
    14041 cagataaaat aaggagatgg aaccatcgtt tcaaagttta atcctcgcca acacatgaaa
    14101 ttatcgattt ctacttgatg caaaagtgta ggcaaattcg cattgaaccc aaacgtgtca
    14161 ggcaaatacc ctaccatcat tggatctcca tatttaagga tcgtatcact gattccaatc
    14221 attaagtttc ttaaaattga ttcagcatcc actaataagg catctgtttg tgcataccac
    14281 ggtcccacga atagttgtcc tgttttcatc agtcgttgga taaccttaag cttttgcgga
    14341 ttgatctcca aatactcatc gacgattgaa gtttgtccat ctaaacaaaa gtttacttta
    14401 gggttccttt ccaattcatc tagaacttca gtaaagacct gatcagacaa tacgatggca
    14461 tcttcatttg taaaatacca ttctctgtcc cagtgcgtgt gttgaattat atgtgcattt
    14521 accatatcag attctcctac tttccttctt ctactgaatt tacttgttgt tcttttttat
    14581 acttgatgga ttttagtgca ttgaccatta acgctgtaac cactccccca ataacaattg
    14641 atactaaata taaaggtata ttcgtaacaa aaggaattgc cataaaggtt gaaagcattg
    14701 tttcttgagt aacaccaaaa aatccattta gaccacatgc tactgcagtg cccaccacga
    14761 tgcttggtat tactcgtaaa ggatctttca cggcaaacgg gatcgtccct tcagtaatcc
    14821 ccacaaggcc cataatcata gctgtctttg cattatctcg ttccgattca tcataaagtt
    14881 ttttgtttaa aattgttgct aaaaacattc caaatggcgg aatagcaatt cctaataaca
    14941 tggctgtact aggtccaaag cttccgcttg aaaaagaagc aaccccaaaa gcatatgcga
    15001 ttttattgat aggtcctccc atatcaaacg ccatcattcc tcctaaaata gccgacatca
    15061 aagcagcatt tgctcctgac atgccttcta gccaattagt cagtcctgtt gtcattgctt
    15121 gaataggcgt agctagtaca taccataata tgattccaac aactaatgta gtaacaaaag
    15181 gaatcagcat aattgatttt agactaataa cttgcatcgg tacttttact tttttcaatg
    15241 cattagctaa atagccacaa gctaatccag caaccattcc tccaataaat cccataccaa
    15301 gattttgaca catcatccca gtgataaaag ctggtgctag accgggacgg tcagcaattg
    15361 aatatgcgat ataggctgaa atcatcggta cgataaactg taatcccgtc tgaccaatca
    15421 ttcgcaggac tccccagaat gtatccgtct ggtcccaaac acctgtgcca ccaaaaatga
    15481 ctgcaataga agttagtaac cctgctgcta cgacaagcgg gatcatatag gaaaccccac
    15541 tttggaaatg atttagaatt tctcttcctt gtttcttgaa ttccattagt aactccccca
    15601 cgatatattt tttatttgtt aattaatgca attgcttcat caatcgtctc ttcaccatct
    15661 tttactacct ctgaggtccc tactgttaaa atcggtttac cgtcaaagcg acctcgttct
    15721 gaaacatttg tatctactgc aaatatgaca acatctgcct cggaaacatc cgaaattgaa
    15781 agtttgtttt ctatccccat agccccctgt gtttctactt taatatctaa ccctttcttc
    15841 ttcgcaaact tttctaaatt agcttttgcc atataagtat gggcaatccc tgcgatacat
    15901 gctgttactg ctactatttt tgtcatatga aattcctcct tttaaaatga tagagctttt
    15961 accaattctt ttttatttct cgaatctttt attcttttaa caaactcgtc atccatcaat
    16021 cttccagata attctgctaa aatacgtaaa tgattagtac ctttatcagt gtcttttatt
    16081 gctaacaaaa agacgagatc aactggttga tcatctaatg cgccccattc aacagaccgc
    16141 tttaacttgg ctacagccac tgttgattca attactgcta ggcttttgcc atgaggaatg
    16201 gcgatatttt tcccaattcc tgtggtcata tggttttctc tgtcaaatac gtctttttga
    16261 aattgtttgt ggtcagacac aaacccatta gtctctaaca atttcgataa tttttcgact
    16321 acactttttt tatcagatgc atttaaatct atcactatat tatttatatt gattttttca
    16381 ctgatcattt ataagccttc tttcaatata taaactaatt tttcaaacga cgaactcgtt
    16441 tttaaatttg ctaataactc atgattcgaa gcgatttcat tgaatgtttc ataaatttta
    16501 ttcaattcag gttttacttt ttcattaagt gcaaaaaaga aaacagaatg aacaagtgtc
    16561 ccatcccaat caattccttc tggattaacg tagacacaga ttactggctt ttttataaaa
    16621 tctacttcga tatgcggcat agcaaaatca ttcatcgctg ttgaagatag tttttctctt
    16681 ttgattgctc cctctagtat cccttctttc gcgtaatttt cttttaccaa ttttttggta
    16741 attatattta aaacgttcac ttgattatcc tttatttggt gggtaaaaaa gcaaaatcgt
    16801 ttatctaata aatttagaaa agcatcttct gtttgttcat ttaactgtag atactgtttt
    16861 acttttctga catctgcccc taacatcaaa ggactaacaa caataacagg aaaatttgta
    16921 ttttcaatcg gaatcgtgct tataacaagt tcatcagtga cttttctatc ttgtagttca
    16981 ttgatgctta gtacatcttc tatctcaacc atatctgcaa aaccgttatt tattctttgc
    17041 tctaataatt ttgatgttcc atatccgcta ctacaaacta atatcacttt cgttttgtca
    17101 ggtttgtaac gatccaaaag tgattgaata tggagtgtta cgtaagcgat ttcatcttca
    17161 ttaaaatgta tacagaatct ctcttcgatt tcctctaata aatctacact tatcataaat
    17221 gaccttttaa aagaatattt gattttgtcc gtataagggt tatagatatt tactcccaat
    17281 ttgagtcttt taactgctgc attgagatgt aatactagat tattgatcaa ttcttcatca
    17341 ggataaaatt catttaattt aattcttact aactcttgta gctcaacacc catatctaaa
    17401 taggaaagct tcgtttgatc taagtcgtga ttcatagcat ttttttcaat cgaagtaatg
    17461 tgaatgtcaa cataagcttg ttcatctatt ggcacaataa ttgaaaatgc tttttcaatc
    17521 attttgatta aaataagtgt attctttgag atttcttctt cagaattatt actacgtgat
    17581 aagaaattgc ttgtttcaat cctttctatc gcaatagcaa ggtggatagc taaagtttga
    17641 aattcataat ctgtaaattt caattgtgtc ttattcatgt atcgattaag caattcggct
    17701 acttttttca atatgtcctt atttaagaaa ttattcgctt caccaatgaa attgatattc
    17761 aagtctaaga gatctgtttt actgttagat gctgtaaagc caccccaata atagttcaca
    17821 acttctgaca tcaaccttct tttacttttt tcatcagctt tgatgcgcac gccatttcga
    17881 tctgaaacta aggacatccc atgcttgttt agtctttttc taacatcttt cattgtgctt
    17941 tctaccgtcg acctgctcac gtataattcg tcagcaagat tttgaatctt tagagaatca
    18001 ttagtattca ataatttagc aaacacataa gttagacggt cctccggaga gtctgcgata
    18061 ttgattttat gactggataa taaatttcga agtgtttctt tttttccaat aagcgaaacg
    18121 ccttttttag gttgaaccaa catttgtaca tctggcattt tttggattat ttctttcaaa
    18181 attaaaatat ctttgctaat tgttttcgta gatacatata attgttctga taattctttg
    18241 taagttgtgt gctcattttc aatcaaaaag tatagtattt tcatttgccg ttcgctaaat
    18301 tcgatgccca ttatcttcac ctcctgaata cagcttaagt gtacaattat tatctctatt
    18361 ttcatacaga agaaagttct tgattaatgt agaagtttct aatattcttg attatttcaa
    18421 atcaataaaa aagcctgcaa tatttatatc acaggctttt ttctctatta aatatatata
    18481 agataataca tttttctact agttctcact aatcttgtat aattaaaccc tccgcaactt
    18541 gacggagatg gagaagatgc ccccgtctaa tctagcggtc atctctcctc ccattccttc
    18601 caccaatttt tttgacaggt aaagcccgat tccgctagat tccgtatttg tgcggctggt
    18661 attttcggta taaaaacgct ggaagacttt ttcaacagag gtttgcgttc ctggtttgat
    18721 aggattggcc actgttaatc gaatgaatgg atcctctttt gaaaatgtca cctttgcttg
    18781 tccatctgca taacgcaata aattctgtat gatattttgc agcactcgca gcaacatgag
    18841 cggatctgct aatacagttg tatttatcga gtgttcagcg aacgctacct cgatattttt
    18901 ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc acctctttag aaaaagaaag
    18961 cgtatggaga tccatttctt tttgcttcgt ctctattcga gcaagatcat aaaactgatc
    19021 cgtcatactg agtaatcttg caacagattg tttgatgatc gttaaatact tctcccgttt
    19081 ctccgtatcc tctacctgtt ccagtaattg tacataaccg ttaatagcag tcaaaggagt
    19141 tctgagatca tgagaaaggc cgacgatcga tagtctgatg ttttcttcca tttcttgaaa
    19201 tgcttgtcct ttttgttcat tttcatccaa ccatgtattc agtgcatcta ctaggtccat
    19261 tagattcttt tctcgaaatt cgactgaaag acgaccacca tattcttgtc gttcagaaag
    19321 agtggcgatt ttcttagtta atctcttgat ttccctttgc aggaagaaaa taaaacttcc
    19381 cataaagata acaacgagaa gcaacagcca tttaatcaaa tctaaacccc actccccaca
    19441 ccgtatgaat atacacagca tgtgcctttg ctagcttccc tcgaagattg gaaatatgca
    19501 tattcactgt tttctcacta tcaaaatatg gttcttgcca aactgtttca taaagatttg
    19561 ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt cagcagatcg aattctcttc
    19621 ctgtcaaatg gacagccaca ccattcacat ttacatccct cgactctttg tccagtaaga
    19681 tttccttgta atagatgcgt tgcgacagat cttttacttg gacagaagtc tgatgacgaa
    19741 gttggatgcc gattcgtaac aaaagttctt cgatatcaaa tggtttcgtc acatagtcat
    19801 ccgctcctgc tgccaacaag gaaactttat ctctttgatc gctttttgct gaaagaacga
    19861 tgatcggaac attcgatgtc tgtcggattt ctgctaacaa tgcttcacct gaaagccctg
    19921 gaagcatgag atctagaagg atcaaatcga caggatatgt gcttaacaat aattttcctt
    19981 ccgtacctga ataagcatca agaagtttgt atctctgttc aagaacctcc tccagtaagg
    20041 aatgaacagc ttcatcgtct tcgataatca aaatagtttc cagcaggacc acctacttaa
    20101 tgtcagattt ttggaaaata aacattccta gagctgatga taccacgatt gttactagac
    20161 tgaccagaat cattttcggt gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca
    20221 tgagccacgt ttccgtaaac cactccgtta gaaattccaa gcctttgatt tttgcgaaaa
    20281 caccgggaat gaaggccgtt cccatgatat aaagaaaaga aagaagcaaa ctgacactgg
    20341 tacttctagt gatgaatgtc aaagccactg gaacagataa tgtccctaat gtcagcagaa
    20401 gcaatacagg aaatactttt agaacataat ttccatagtt tttgattccc tcaacttcac
    20461 caaatccgaa agcaagtgtg taagcaagca tggctagtcc cgtgatcaaa accacgccaa
    20521 gtgttgtgat caaaagcgta acgagaagtt tcgacacata ataagtcatt cgattccgtc
    20581 ctaaagataa cgtattgcga atcgtaccgt tttgaaattc ttccccaata taaaagcacg
    20641 caaatgctgc gaaagccaga agaatccatg cagccatttt ttctaccatt aaaatccctg
    20701 ttgcaccgtt gatgactgta tccgttgctt ggtttaattc attgccgtat tttgtatgca
    20761 tataccattc gttccaagaa taaaaaagcg gaagtacgac catcagtcct agtgagaccc
    20821 acattttacg gctcttccat acttttattt tttcagcagt taatagatta aacatgtgga
    20881 ttgcctccag ttaattgtaa gaaataatct tccagttttt gcccaactag gtgaatacca
    20941 tccacaggaa cttttgcttc taccaatgct tgattgatgg ctgctacttg gtcaattcct
    21001 tcaaaaagct ggataacacg gctagattgt acaaaataat ccctataacc aagagagtcg
    21061 agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt ttataaattg acgagattct
    21121 tgtgccaatt cttctttcga taattctttg atcaagtgtc cttcatgcaa aataccatag
    21181 tgagtggcga tctgtgacaa ttcatctagc aagtggctag aaagcaagac ggtgatgccc
    21241 cgttctttta ccaaacgttg gatgatttct cgcatttctg ccatccctgc tggatccatc
    21301 ccattcgtcg gttcatccaa aatcaaaaat tctggattcg tgatcagtgc atttgcaata
    21361 gctaaacgct ggcgcatacc taaagagaaa ttctttgctt tctttttccc tgtattttcc
    21421 aatcgcatca ttttcagcag gtcttcgatt tctctatccg acacaccgcc gttcgctgct
    21481 tggatacgta ggttatctct tgcagttaat tctggataaa gagcaggggt ttcaatcgat
    21541 tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc caaataattc gatcgaacct
    21601 tcatcaatcg agatcaaacc cataatagtt ctcataaagg tagacttacc cgctccattt
    21661 tccccaatca atccatagat cattccacgt ttgatttcga ttgatacttt atctaatgct
    21721 ttggcagcac cgtatttttt ggttatacca gttgctttca agaccgtttc atttccaaaa
    21781 tccatactta tccttctttc ttattgatga gtgaagtata gttgacgggt ataaagaaag
    21841 cagccagaaa tgtataaaga aagtgtaaat tttccaattg aatgtcaaaa aaaacaatcg
    21901 gacaatacgt gtctttccta ttatcaggca gtttgttata cgcttgtatc agtttcctga
    21961 ttctttcttt tcgaccaaag tcgtttctct gtattcctct tcactgaaaa tttttgcttc
    22021 tttcagatca agcttgagta attttaccag accggttata aggataacta tagcaagact
    22081 ccctcccaga aaaattagac tgtctcgcag acgtggttgc gttgtcttcc aatcaaagag
    22141 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
    22201 cataagtcgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
    22261 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
    22321 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
    22381 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
    22441 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    22501 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
    22561 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
    22621 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
    22681 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
    22741 tgagaactgt gaagctttac gggctttctt aagacctggt tttttcttcg tatcattgaa
    22801 taagtttgaa aaaccttgat ttataaaggt tttcccgtaa atatatttta ttatatttga
    22861 ttaaatttga aatgtttgtg ttctaaaatg tgttctaatt taaaggcagt atatttcgta
    22921 ttttagatta aatttttagg ctcattcatt ctcaaatcca ttctattcag tcaataaaaa
    22981 ttttaaaaag actttataaa caaagatgtt caagcgagca tcttattttt ttgtgcttaa
    23041 tttgaaagga gaacatttca tgtataaaaa acaaaaaaag aaattatgtg tattaactat
    23101 tttgagtttg gctgttcaag caattgttgg agcaattgct cctacgatta cttttgctga
    23161 tgaaattacc catccacaaa cggtaaccgt agaactagat ttagcacatc aatatgctgt
    23221 tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc actgttccgc attatgcagt
    23281 gtacaacggc tttaaacaag atattttctg tattgaacca ggagtcccga tttacaatga
    23341 gttcactcct ggctatgaga aaaacccgtt accagatatg tccgataaag ccaaattagt
    23401 ttccgtttta tggaaaaaag taggtacaga tgcagatact catattgtgg cacagaagat
    23461 gatctggcaa gaagttaatg ggtataccct tcactcaata aaacgatcag atggtagtgc
    23521 cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc attgacgatt atcagaagaa
    23581 accaaccttc cataatagta cggctaaaac agtattaggg caatcgacta ctgtgacgga
    23641 tacgaataat ctgaatttgt cagagttcga tgaggttgtg gaaaatgccg caaacattga
    23701 ttatcgtgtg aatggtaatc agttgataat caccccaaat gccaattcaa aagaaagtgg
    23761 tgtgctgacg cttaagaaat ctgctggtac cggaacacct gtcgcctata aaaaggctgg
    23821 ccaacagact ttaatggccg gggcgattga taaacctaat acctacacaa tcaaaattga
    23881 tgtggaaacg gagggttctt taaagatcaa aaaagtcgat aaagaatcgg gtgatgttgt
    23941 accaggaacg gttttccatt tagactttgg aaaaacttta cctgcaaaag acgtgactac
    24001 tgacaaagaa ggcattgcta cattggatga gattcctcat ggtacaaaag taaccattac
    24061 cgaaaaatca gtaccggcgc cttatacgat tgataccacc cccatgacta ccaccattaa
    24121 agcaggcgaa accatttacg tgacttcaaa aaacgcacgg gaaaaagggc aaatcattct
    24181 ggataaaagc ggagttgaaa cagggagtga tctttggaat gacaattatt ccttagctgg
    24241 taatacgttt gccattcgca aagacagtcc aaccggtgaa atcgttcaag aaatgaccac
    24301 ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct aatgcgttgg aattgggaac
    24361 ctattatgtg accgaaacta aagctagtca tgggttcgtg aatacgttca aacctgtaaa
    24421 agtcgaattg aagtatgcca atcaaaccgt agcccctgtt accagtaacg taaaagggca
    24481 aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa gacaaagata ctggggataa
    24541 agcacaaggt aaggcagtct ttgagggtgc tgaatacacc ttgtttactg ctaaggatgg
    24601 aaaagctgtt aaatggagtg aggcttttaa accagagtta gcgaagggaa cgaaaggttc
    24661 tgatgaaacg gtcactttga ctttagacga aaagaaccaa gttgccgtta aacatttagc
    24721 tatcaacgag tattattgcc aagaaacaaa agcgcccgaa ggatatagcc ttgatgaaac
    24781 aaagtatcct gtatccatca aaaaggtgga tgatagcgaa aaaaatgccg tgattacccg
    24841 aaatgttacg gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg
    24901 atcagctgcg ggcactaccg aaactggctt caatgatctg acctttaaag tgtcgccatt
    24961 ggaagggacc aatgaaatca caggtgccga agatgaagcg accacagctt ataacgagca
    25021 attagggttt gatggttatg gcaagtttga aaatcttcct tatggggatt atttacttga
    25081 agaagtagaa gccccagaag gttttcaaaa gattacgcca ttagaaatcc gttctacttt
    25141 taaggaaaac aaagaagact ttgtgaagag tgagtatgtc tttaccatta ccgaacaaga
    25201 ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag aagctgacga acaaagcatt
    25261 ttctgttagt ttgaaccgtt taatgctcta tgatttgcct gaggaagaag atagtttaac
    25321 ttctcttgcg acttggaaag acggaaacaa agaattaact tctctggatt ctaccgagct
    25381 agttgataaa ttgagttaca atctacatga aatcaaagaa gactggtatg tggtagcgca
    25441 agccattgat gtggatgcga caaaagctgc ccaagaaaaa gatgaaaaag ccaaaccagt
    25501 ggtgattgca gaaacaagcg caacgttggc gaacaaagag aaaacaggaa cttggaaaat
    25561 tcagcataaa ttaaccgccg aacaagtttt aaataaaact attgtcttat tcaactatgt
    25621 gtatgaaaat aaggaagctt ttgaagcagg cgataagcca gtagcgaagg atgttagctt
    25681 gaataaccaa gcccaaactg tcagttgtac agtggaacac catgtttcta tccaaacaaa
    25741 ggcccaccta gaaaacggtt ctcagacctt tactcatggt gatgtggtgg atatgtttga
    25801 tgatgtgtcg attactcatg atgtactgga tggctcaaaa gaagctttcg aaacaattct
    25861 gtatgcttta ttaccggatg gtacaaacaa agaaatttgg aaatctggca agattgatta
    25921 tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg aaaaaagtag ataccggaaa
    25981 atatccagaa ggaaccaagt ttacttttgc ggaaatcaat tacgataaag atgggaatat
    26041 caatggaaaa cacaatgaag atttgaaaga aaaatctcaa accttaacac caaaagaagt
    26101 tccaaccata ctaagtacgc cgaaacaacc ggaaacacca actgttccaa gtggctctca
    26161 agaatctagt cccacagtga agaccttccc tcaaaccggg gagaaaaatt ccaatgtttt
    26221 actgttcatt ggttttactt tgatttttgc gacggcgggc tattatttct ggaatcgccg
    26281 gaactaaggt gatgagatga tacaagtaaa tcctaagaac caacgtgcgc gaccgcctcc
    26341 taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca atgaaaagag gaaaagaaga
    26401 tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt tggaaagtta agttatgccg
    26461 gtgaaggaga aattataaca gaaggatatg gacggaatac tacggtgatt ggtcgcagtt
    26521 atcatttgta ttccagtaag caacgggccg atgatattga ggtggtagta gctgcggaag
    26581 ccggtgaaaa ggattttgat caagaccaac cgctaaaagc cgtgaatccc catttggttg
    26641 ccaaaggcta tgagattgag aatcgtgggt ttactgatta cgtgttgtat gttgatgatt
    26701 tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc gtagattcag
    26761 gtttaacatt tgggaagttg cgtttttccg cattacgccg agaagtacgg aagcaaaatg
    26821 aggatggaac aattggtaat gaggtaaaag aacgaaccta taatttaaaa tcctctgcac
    26881 aagggcgaat gattcaagtt agtatcccag ctaatgtacc cttacgcgag tttgcttatg
    26941 atgcagaggt agagttggtc aatccaatcg tggataccgt tgccaattat gtttttcgag
    27001 aaggaactac cgttaattgg ttcattaagg cggatgattt agtcttgaag cggcagccga
    27061 atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata ggtgaagatt atggaaatta
    27121 atgttgaaca tattttagat tgtctcgatc aatatggaaa aggcgagctg actgaggaac
    27181 aactcgttaa agcattaacc tctgatgaaa aaatgtttct gattatacat caaggtttac
    27241 ttggagaaaa tagcgacagt gaagaagact ctgatgtact agactggtta ggagaagaag
    27301 agtctttctt tatagttgtc gaagtaaatg aaaacttatg tcggcaggca gaatccattt
    27361 tggaagaaat tggtgtggag atgccggatg ctattgaagg ctttttaaat caattggttg
    27421 aaacgaaaca actcccctcc tcgtagcagt aaatgactag cataactttt tgaaattgcc
    27481 taatgaaatc ctattgggct ttttcgattg gaggtggaaa ccatcaaaat gtacaaagga
    27541 catcgcatac gcgcaggaga tcagcacttg gtttatcact ttgttcttgg ctggctactc
    27601 gctctgttca tcggttggat gagcgtcttt tattttcaag agtttagaca atttgatatt
    27661 tctaagctat cactttctac tattgaaatc gtttggtcta taaaagattt agtatgtctg
    27721 cttggaagtc tcgctttttc aggagctatg atattgcttt atattcactt ttttcttgat
    27781 cattggcgaa gcttgtggca tcgacaaaag ctagctcgga tgattctaga gaaccattgg
    27841 tatgaagtga aacaaactca aagtgaaggc tttttcaaag atttgaatag tagccgaacc
    27901 agagagacta tcagttactt ccccaaaatc tgttatcgaa tgaaagatgg attactgtct
    27961 attcgcgttc aaatttcact tgggaaatac caagatcagc ttttaaaatt ggaaaagaag
    28021 ttagaaagcg gactgtattg tgagttagta gaaaaggaac tcaaagactc ctatgtggag
    28081 tatactttac tgtatgatat gatcgccaat cgaattggga tagacgaagt agtggcagaa
    28141 aatgggactc tgcgattgat gaaaaatcaa gtatgggctt atgattcttt accccatatg
    28201 ttaatcgctg gtggcacggg tggagggaag acctatttcc tcctcaccat tatcgaagca
    28261 ctattgaagt cagatgcaga attgtttatc ctcgatccca aaaatgcaga tttagctgat
    28321 ttaggtacgg tgatgcctca tgtttattca caaaaggaag aaatttctgc ttgtgtggaa
    28381 gatttctatg aacgtatgat ggctcgtagc aaggcaatga aagaaatgtc taactacaaa
    28441 acaggagaga attatgccta tcttggactt ccacccaact ttttaatctt tgatgaatac
    28501 gtggcttata tggagatgtt aacgacgaaa gaaagtgcgg tgattttgaa taagctcaaa
    28561 caaatcgtga tgttgggtcg tcaatctggt ttctttctga ttttggcttg ccaaaggccg
    28621 gatgcgaaat atttaggaga cggaatccgc gatcaattca actttcgcgt ggctttgggt
    28681 cgtatgagtg aactcggata ttccatgatg tttggggagg tcgaaaaaaa cttctttatg
    28741 aagcacatca aaggtcgagg ttatgtggat acaggtggca gtgtgattag tgagttttat
    28801 acgccacttg taccaaaagg gtatgatttc ttagattcaa ttagtaaatt agaaaagatg
    28861 aatgattgag tgatcttcaa tactgacttt atacttcaag taacgtataa tacttttata
    28921 tagtaggaag aaaggggata attaatatgg aaagtaatga ttcttattta gagtatgcaa
    28981 aagcaaataa ggataagttt atttcttata ttatcaaagg gaaatccccc gaagttggac
    29041 gagacgcagt ttttatggca gggagtcctg gggctggaaa atcagaggtt gccattggat
    29101 tagctgagaa ttatgataac catgtgatta ttgatgcgga ttactttcgc acccagtttc
    29161 ctgattataa tggaaaaaac tctagtctct ttcaaaaagc atcttcttgg ttggtagagc
    29221 aggcatttag atatgtctta gatcatggct attcttttat tctggatgca acttttgcaa
    29281 tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa acattttcga gtgacgattt
    29341 tctatgttta tcaagaccct aaaatcgctt ggaaatttac acaagaacgt gaaattgcag
    29401 agggacgtca tgtgccgaaa aaggcattta ttaacgcttt tttcaaatca agagagaaca
    29461 ttgcaaaagt aaagagtaaa catccagatg tattgcttca tatcataata aaagattata
    29521 cgaataatat ttccgaagtt cattatgcta cggataatct agaattattt ttaccactag
    29581 ggtatagtag gaaagatttg gaggttcaat tacatgactg agaaagaaca agtaacgaaa
    29641 attgtaaaga aatacaataa gagcattgcg gatttatcgg aaaatgctac tgcaaaagag
    29701 ttcaaaacgg tgattaaata tgtagccgat caggccaatg aaaaacaacg gaaattagtt
    29761 ggattaaata aaaaatagta ggacaaatta ataatgtatg agccaagagc aaaatgttct
    29821 tggctttttt ctttgcaatg aatgaggtaa tgtatgagat ttttagattt attcgccggt
    29881 attggtggct ttcgtttagg tatggaacag gcgagccatc attgtatcgg tttttgtgaa
    29941 attgataaat ttgctagacg tagttataaa gcaattcatg atacaagtaa ggaggtggaa
    30001 atgcatgaca tcacaagtgt atcagatgag tttattcaat ccctcggacc agtggacatc
    30061 ctttgcggcg gatttccgtg ccaagctttt tcaattgcgg gaaagcggca aagattttcc
    30121 gatactcgag gtactttatt ctttgaaatc gctcggttca ccactcttct ccgacctaag
    30181 cttttattcc ttgagaacgt ccgaggattg ctcaatcacg aaggaggggc tacgttcgag
    30241 acgatcctcc gaacgctgga tggattgggg tatgacgtgg agtggtaggt gcttaactca
    30301 aaggcctaca ttccccaaag ccgggagcgg atattcctta tcggacattc tcgagacgca
    30361 tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa catctgatca aaacatcaga
    30421 aatttggtaa atatcaatcc ttccaatcga ggaatgggtg gacaagtcta tggttcagat
    30481 ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa tcgcactgcc agcaagggat
    30541 ggaatctctg tagcaggact attaccggga aagtttgaac aaggtaatcg agtttatgaa
    30601 gttactggaa ccgcccccac cttgtcaacg aaacaaggtg gtacaaaaat catgattcga
    30661 aaaaatgaaa cagaatatca agaggtgaag ccagaagaca gtgtgaatct ggcttttcct
    30721 aattccacaa gtcgccgagg tcgtctggga aaacagagtg ttcatacctt actaacagga
    30781 gaccaacaag cgatcgtcac ggatcagtat caaattcgga agctaacacc tagagagtgt
    30841 tggcgacttc agggctttcc cgattgggct tttgatcgag attctcaagt caattcagat
    30901 agtcagttgt ataagcaagc cggaaactct gtaacagttc cggtcatttt tgatattgcc
    30961 agaagattaa aagagttgga tgtacatggt ttatgatgag ttgattggag aaatttattg
    31021 ggtgattgga aagattcagt cagacccaga attagaagaa caattgcgac ggttaaaatt
    31081 tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca tatttaatga atgaggagac
    31141 ggatgctcga attgaaataa atcaagtaat tgcagaattt gagcggattg cggaccaagc
    31201 aaaagaacca gatattcgtc aatacttgtt agaaatgaaa gcagagttgg aagtaaatgg
    31261 tattacagat gagtaaccta aaccaacaca actgactgtt aggcgctgac cgcggaggat
    31321 tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat ggacggcgtt agccgaccat
    31381 gatgtctcac ccccgtatct aacagggggg tacaaaaaca caaaaaataa gtaagcaacc
    31441 attaagaatg cttgttgtat caaagatagt aagtaatttg tataggtgtt aactcatttg
    31501 aaaaagttaa caccttgaat ttggaggtga gaacttggca caacggaatt tagattatcg
    31561 gctattaaaa gaccgacgca acgaatacgg cgtttcacaa aataaattgg ctacggcttg
    31621 tggacttagt cggccgtact taaatcagat agaaaatggt ggcgtaaccg catctataaa
    31681 aaccatgagg aaaatttttg accaactgga aagctttaat cccgatttgc ctttgacgtt
    31741 actgtttgac tatgtaagga ttcgctttcc cacaacggat gcacgaaaaa ttattcaaga
    31801 aattctccat ttgaaatttg agtatatgct ccatgaagat tatgcctttt actcctatca
    31861 agaacagtat gtcatgggtg atattgtggt gatgttgtct catgaagaag ataaaggtgt
    31921 gcttttagaa ttaaaggggc gaggttgccg acaatttgaa acttttttac tcgctcaaaa
    31981 gcgtagctgg tacgactttt tcgaggattg tctaaaggcc ggtggcgtga tgaaacgctt
    32041 ggacttggca atcaatgatc gagtggggct attggatata cctgagctaa cgaaaaaatg
    32101 tcagaaggaa gaatgtatct ccttgtttcg tacctttaaa agttatcgtt ccggggaact
    32161 tttaaaggct gatgaaaagg acggcatggg caatacctta tatatcggca gtctcaagag
    32221 tgaggtttat ttctgtttgt atgaaaaaga ctatgaacaa tatatcaagt tagggattcc
    32281 attagaccaa actgagacga aaaatcgttt tgagattcga ctgaaaaatg accgtgcttt
    32341 tcatgcgatt caagatttgt taaaaggtcg tagcattgag agcaccacgt tttccattat
    32401 caatcgttac ttgcgatttg cggataaagt agaaggtaaa aggcgaacca actggccctt
    32461 gaatgaacaa tgggggcgtt ttattggacg gaatcggaaa gagattcaat taacttccga
    32521 gccaaaaccg tataccattg aacgaacctt gaattggcta ggccgacaag tcgcccctac
    32581 gtggaaaatg gctaaggagt tggatcgatt gaatcggaca acctatatcc aggatatggt
    32641 acagaatgca cgcttatcag accgacataa aaagattttg gagcaacaaa gtatggcaat
    32701 tgaaaatttg attatatgaa aagaggaatc gtcatgggaa ttgtaaaaga tatttttttg
    32761 gtatttggtg gcagtatgct tggtgttact gtgatgtgct tgatgaatgc aagtgcagca
    32821 gcggatcgag caatggaaaa gaaaggaaag tgacaagatg aatttcggac aaaatttatg
    32881 tgcgactcgt tccttagtga aaagctaagg cactaataaa agaacgaaaa agtttggtta
    32941 ttaggagaac tgataatctg acaggtggaa tgatgaagta acgctctgaa acgcctgccc
    33001 tgagcctccg acaagcattg tatgaggaaa ctcagaatgt ttgaagctcg gtaaagacgg
    33061 ctgaaagata ccgtaatagt tgatgaacat atatcttcta tcgaaaacta tccagaggtg
    33121 ggtagcgtat ccaacaggtc gggggtctat aaaatatcta tggttagaat gttctataag
    33181 gaactgacga aacagcgaat gtacgggtct aaatttgaac tttgtagaaa tgcaaaggta
    33241 cgagaaatcg tagctggtgt ggcaaagtaa gaatataggt tatgaaatgc catataacgt
    33301 tacaggcggt atcaagccag caggcttata gctagtgacc taaggatata tatgtataga
    33361 taagattatc ggaacgagga aaggtattgg gttctcatca agagaataaa ctgacgaaga
    33421 acaataagca gttgaactaa tgctgaaaag cagagggcga acctaagaaa ttcttgtaat
    33481 gagaatggag gaatgccctc aagtcgtgta tatattagaa aagtattatg ttgtttcaat
    33541 aaggattacg agtaagataa aaaagctcac tcaatttcaa gtaaaggagt gaagccttat
    33601 gcctaaaaat aaaaatgaaa cattgtgtgt agaagattta agacacgctg aatattacga
    33661 aatgcaaaac acctttgatg atttatatgc caaaagtaag aatggagata ttttcactca
    33721 tcttatggat attatcctat cgagagaaaa tatattactg gcatatcgaa atatcaaagc
    33781 aaatgcaggc agtaaaacag caggaacaga cggtacaata attaaagata ttggcaaatt
    33841 gccagcagaa acagttgtca aaaaagtaag atacattgtt gcaggcagtc cacatgggta
    33901 tcgacctaag ccagtgaggc gaaaagaaat accaaaacca aatggtaaaa ccagacctct
    33961 aggtattcct tgtatgtggg acaggcttat acaacaatgt ataaaacaag tattagaacc
    34021 aatctgcgaa gctaaattta gtgaaaacag ctatggtttt agacctaata ggtcagttga
    34081 aaatgcaata aaagcaacct ataaccgcct acaaatcagc cagctacatt atgtgattga
    34141 atttgatatt aagggtttct ttgacaacgt gaaccattcc aagctgataa aacagatatg
    34201 ggctatggga atacgggaca agcacttgat attcatttta aaaaggatat taaaagctcc
    34261 aatcaaaatg actaacggta caataacata tccagagaaa ggaactccgc aagggggaat
    34321 aatatcccca ctactagcaa acattgttct taatgagtta gaccattggg tagaaagcca
    34381 atggcaggaa aatcctgtta caaaaaacta tgtggttcat atcaataaaa gcggtagccc
    34441 atgtaaaagc aacgcttaca aagaaatgaa gaaaacaaag ttaaaggaaa tgtatatggt
    34501 tcgttatgca gatgatttta gagttttctg ccgctacaag gaaagtgctg aaaaagccaa
    34561 aatagcaatc actcaatgga ttgaacaacg attaaaattg gaagtatcac aagaaaaaac
    34621 aagaattgta aatgttagaa aaagatactc cgactttctt ggtttcaaga ttaaaatgat
    34681 accgagaaga aagaagttag ttgttaaatc acatataagt gataaacaat tcaaaaacca
    34741 aaaagacaag ctggtatcac aggcaaagaa aattgcttct ccctcaaaag ggaaaacgga
    34801 attagaagaa acaagacttt ataattcaat ggtattaggt atgcaaaatt attatcagat
    34861 tgcaacgaat gtaaatctgg attgtatgaa actgaataga gctgtaatgg ctatcttcac
    34921 taacagatta ggtacaaaca aaaggggcag attgagaaaa acaggcagac aacttacgac
    34981 aaaggaggca acaagatatg gtaaatctaa aatgcttcgg tatgttgctg gcgagggcga
    35041 acctattttc cctgttggat atatccaaca cagaaaacca atggcaaagg tgtatttagc
    35101 gaattgctac actctagagg gaagaaaatt tatccacacc aatctaagtg ttgataaatt
    35161 actgatgtac cagttaatga aattatcttt ggaaaatcgc acaatagaat acgcagacaa
    35221 tcgaatttcg ctattttctg ctcaacatgg aaaatgtgct ataactctgg aagaatttca
    35281 acaagctaat gaaatacatt gtcatcatat tgtaccaact ggagctggtg gaaatgacga
    35341 ttataaaaat cttattttgg taaaagaggc ggtacaccgt ctaatacacg ctaaaacaga
    35401 agaaacaata caaaaatata tagctctatt gaagctaaat aaagtccagc tcataagact
    35461 aaataaatat agagtattag ctggcaatca agaattaaac ttgatatagg tctactacat
    35521 aaatactttt caatataaac acgatggaac gcggagtgcg gtgaaagtcg cacgctccgt
    35581 gtgaatgggg ggaaaagttt gagatattta taatatcagg aacttaccta tccatatatc
    35641 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
    35701 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
    35761 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
    35821 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
    35881 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
    35941 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
    36001 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
    36061 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
    36121 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
    36181 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
    36241 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
    36301 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
    36361 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
    36421 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
    36481 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
    36541 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
    36601 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
    36661 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
    36721 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
    36781 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
    36841 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
    36901 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
    36961 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
    37021 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
    37081 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
    37141 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
    37201 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
    37261 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
    37321 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
    37381 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
    37441 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
    37501 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
    37561 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
    37621 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
    37681 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
    37741 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
    37801 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
    37861 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
    37921 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
    37981 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
    38041 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
    38101 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
    38161 attcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
    38221 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tgg
//
LOCUS       DAFGFZ010000011        37963 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000011 DAFGFZ010000000
VERSION     DAFGFZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.011"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            90..3368
                     /locus_tag="LR726_000482"
     CDS             90..3368
                     /locus_tag="LR726_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL6215592.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3909..5816)
                     /locus_tag="LR726_000483"
     CDS             complement(3909..5816)
                     /locus_tag="LR726_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL6215593.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5946..6821
                     /locus_tag="LR726_000484"
     CDS             5946..6821
                     /locus_tag="LR726_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL6215594.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6901..7875
                     /locus_tag="LR726_000485"
     CDS             6901..7875
                     /locus_tag="LR726_000485"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL6215595.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8055..9200
                     /locus_tag="LR726_000486"
     CDS             8055..9200
                     /locus_tag="LR726_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL6215596.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9337..9681
                     /locus_tag="LR726_000487"
     CDS             9337..9681
                     /locus_tag="LR726_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL6215597.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9952..11238
                     /gene="efmA"
                     /locus_tag="LR726_000488"
     CDS             9952..11238
                     /gene="efmA"
                     /locus_tag="LR726_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL6215598.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11376..11975)
                     /locus_tag="LR726_000489"
     CDS             complement(11376..11975)
                     /locus_tag="LR726_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBL6215599.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12379..13500
                     /gene="mnmA"
                     /locus_tag="LR726_000490"
     CDS             12379..13500
                     /gene="mnmA"
                     /locus_tag="LR726_000490"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL6215600.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13746..15167
                     /gene="arcD"
                     /locus_tag="LR726_000491"
     CDS             13746..15167
                     /gene="arcD"
                     /locus_tag="LR726_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL6215601.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15181..16299
                     /locus_tag="LR726_000492"
     CDS             15181..16299
                     /locus_tag="LR726_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL6215602.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16359..17651)
                     /locus_tag="LR726_000493"
     CDS             complement(16359..17651)
                     /locus_tag="LR726_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL6215603.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17783..18361
                     /locus_tag="LR726_000494"
     CDS             17783..18361
                     /locus_tag="LR726_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6215604.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18490..18867
                     /locus_tag="LR726_000495"
     CDS             18490..18867
                     /locus_tag="LR726_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL6215605.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18800..20872
                     /locus_tag="LR726_000496"
     CDS             18800..20872
                     /locus_tag="LR726_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL6215606.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21083..22030)
                     /locus_tag="LR726_000497"
     CDS             complement(21083..22030)
                     /locus_tag="LR726_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL6215607.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22276..22740
                     /locus_tag="LR726_000498"
     CDS             22276..22740
                     /locus_tag="LR726_000498"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL6215608.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22802..23845)
                     /locus_tag="LR726_000499"
     CDS             complement(22802..23845)
                     /locus_tag="LR726_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL6215609.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24222..25187)
                     /locus_tag="LR726_000500"
     CDS             complement(24222..25187)
                     /locus_tag="LR726_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL6215610.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25421..26266
                     /locus_tag="LR726_000501"
     CDS             25421..26266
                     /locus_tag="LR726_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL6215611.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26457..27644
                     /locus_tag="LR726_000502"
     CDS             26457..27644
                     /locus_tag="LR726_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL6215612.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27650..28006
                     /locus_tag="LR726_000503"
     CDS             27650..28006
                     /locus_tag="LR726_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL6215613.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28008..28346
                     /locus_tag="LR726_000504"
     CDS             28008..28346
                     /locus_tag="LR726_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL6215614.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28422..28678
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28741..29775
                     /locus_tag="LR726_000505"
     CDS             28741..29775
                     /locus_tag="LR726_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6215615.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29768..30454
                     /locus_tag="LR726_000506"
     CDS             29768..30454
                     /locus_tag="LR726_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215616.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30478..31326
                     /locus_tag="LR726_000507"
     CDS             30478..31326
                     /locus_tag="LR726_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL6215617.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31523..32296
                     /gene="sufC"
                     /locus_tag="LR726_000508"
     CDS             31523..32296
                     /gene="sufC"
                     /locus_tag="LR726_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL6215618.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32309..33595
                     /gene="sufD"
                     /locus_tag="LR726_000509"
     CDS             32309..33595
                     /gene="sufD"
                     /locus_tag="LR726_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL6215619.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33592..34827
                     /locus_tag="LR726_000510"
     CDS             33592..34827
                     /locus_tag="LR726_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL6215620.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34814..35284
                     /locus_tag="LR726_000511"
     CDS             34814..35284
                     /locus_tag="LR726_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL6215621.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35289..36680
                     /gene="sufB"
                     /locus_tag="LR726_000512"
     CDS             35289..36680
                     /gene="sufB"
                     /locus_tag="LR726_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL6215622.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36778..37920)
                     /locus_tag="LR726_000513"
     CDS             complement(36778..37920)
                     /locus_tag="LR726_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6215623.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT        12337 a         6989 c         8072 g        10565 t
ORIGIN      
        1 tattataatt ttgtttttta ttgacagtaa aaataaatta ggtaatactt taagtaaacg
       61 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
      121 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      181 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      241 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      301 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      361 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      421 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      481 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      541 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      601 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      661 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      721 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      781 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      841 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      901 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      961 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
     1021 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1081 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1141 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1201 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1261 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1321 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1381 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1441 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1501 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1561 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1621 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1681 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1741 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1801 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1861 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1921 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1981 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     2041 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2101 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2161 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2221 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2281 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2341 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2401 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2461 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2521 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2581 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2641 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2701 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2761 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2821 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2881 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2941 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     3001 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3061 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3121 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3181 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3241 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3301 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3361 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3421 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3481 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3541 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3601 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3661 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3721 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3781 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3841 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3901 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3961 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     4021 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4081 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4141 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4201 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4261 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4321 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4381 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4441 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4501 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4561 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4621 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4681 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4741 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4801 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4861 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4921 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4981 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     5041 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5101 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5161 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5221 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5281 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5341 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5401 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5461 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5521 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5581 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5641 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5701 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5761 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5821 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     5881 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     5941 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     6001 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6061 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6121 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6181 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6241 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6301 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6361 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6421 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6481 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6541 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6601 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6661 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6721 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6781 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6841 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     6901 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     6961 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     7021 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7081 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7141 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7201 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7261 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7321 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7381 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7441 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7501 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7561 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7621 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7681 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7741 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7801 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7861 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     7921 aaggagctgc gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc
     7981 tagacaaaaa tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca
     8041 ataatttgga agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc
     8101 ccatgcatcc acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc
     8161 cttcaagcat acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa
     8221 tcattgcaga cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag
     8281 aaggggataa tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc
     8341 taatcactac agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa
     8401 aggggtttga cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg
     8461 tcaaaaaagc gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa
     8521 tcggaaattt gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt
     8581 tccatacaga tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa
     8641 tcgatttatt aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat
     8701 ataaacgaga cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc
     8761 ggcgtgcagg tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc
     8821 tgtcacctga agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc
     8881 ttgatacatt agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac
     8941 tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc
     9001 ttgatcttaa tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc
     9061 cttcacacgt attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc
     9121 gtatcagctt tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag
     9181 ttcgtgctgt caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa
     9241 cgccttcatt ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta
     9301 taataaagag aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg
     9361 gtactgggcg caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc
     9421 gataatggat ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc
     9481 acgccgcaaa gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca
     9541 ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca
     9601 aaacagaaaa tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg
     9661 ttattcgaaa aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct
     9721 attgttagat gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa
     9781 tttaacacat gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt
     9841 taaattgaaa cgattgttat ttagggaaaa aggtattttt tgaaaaaaga atcccgtata
     9901 atgattgttg tgcaaacgct atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat
     9961 gaacagtcgg tcgttttaac gaattggaag agaaattatt tgttcttttt atcgggacag
    10021 tttttgtcag gaatcactag tatggtcgtc caatatgcga tcatctggta tttgacaaga
    10081 gaaaccggtt cggcgacaat tttgagtttt gctactttac taggaatgat tcccatggtt
    10141 ttactaagcc catttgtagg accgctagtg gatcgctggg ataaaaaagc tcttttgatt
    10201 gtcacggata ttattgtagc gattttcgcg cttattttag cagtagtcgg aacgatttca
    10261 gaatccttcc cgatttggct agtttttgtg tctttgttta tgcgttcggt cgcacaaaca
    10321 ttccaaatgc caacgatcca gtcgattatg ccgacaatcg ttccatcttc tcacataaca
    10381 aggacgaatg gacagttagg aatggtccaa tcagcgaatt tcatcattgc gccagctcta
    10441 ggtgcggctt tgttttcagt cgttcctgtc aattacctta ttttattaga tgtattgggg
    10501 gcagtttttg gtgtcggctt attgattttt gtgaagatcc caaaagtttc tcctgaaata
    10561 ttggaagttc cgcttactat ttttaaagat gcaaaatttg ggctgcagca attgatggat
    10621 aataaagggt tatggtacat aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca
    10681 gcaatcagtc tgtatccatt gatgacgcta gattactttg gtggttccgt tggacaggcg
    10741 ggagcagtgg aagtagtcta cgcagtcgga atgctcttag gcggtgcact gatcagtttt
    10801 ataggaacat ggaaagaccg aatgaaacct attattatag cctacatcat tatgggcctg
    10861 acgatcggtg caagcggatt ggttccaaac gatagtcaag ggttcttgta ctttcttatc
    10921 ttgaatgcag gagcaggttg tgcaacgcca tatttcaata cactgctgat ggcaatgatc
    10981 cagcagagtt atgaatcgaa tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg
    11041 aacctggcag gtccaattgg attattattt gcaggaccat tagctgatcg tttaggggta
    11101 gaaaaaatgt tcttgttttc cggaatcggg attttattat gcggaatcgt tttattttta
    11161 acgtcagctg cccgaaatta tgataaagaa ttacagaaaa aactggtaaa agaacaccat
    11221 gagcaaaaag acgaatagta tggaatggag ttgtgacaga agtcttgtta caactccatt
    11281 tctgatccta atgttcctgt ggcaataagc ctactcaaat gaaaaatatg agtaattatc
    11341 tttatttgtt taatacttga tatagggcag ttttttcacg acctatcgta cttcctcttt
    11401 gcttcataga tttctttgat ttgttgctca gataacggag tgccaaatgg tagtttttcc
    11461 tttaagagtt ctctttccgg tgcgtctacg cctacattga catcgttctc gataggtacg
    11521 ctgtaatgat ggatatgtcc atgaagattg cggatagtgt cgttaggccc gagcaacatc
    11581 ggataatgag tcaagtaata ttgttgatgg tcgaatttaa cgatggcacc tacatcataa
    11641 aaataaaatt tgggacttcc gtcagtaagc ctaggatcat ttttttctaa ataacggaaa
    11701 aaagcgcgac tgtcatggtt ccctttgatc aagtggattg ttccttgcag ttgagtgacc
    11761 agttccaaaa tttctgcatt tgctttttca tattgcggat gcatagcaag gtcgcctaga
    11821 tgataaacat ggtcggtttc ttttattact gcattccagc tagtgatcaa ctgttggttc
    11881 atttcatcca catttgcaaa gagccgcgga gcaaagtcgt tttttcctaa taagcgttca
    11941 tggaaaaagt gcatatcact gataaagtaa ttcatgtatc ttttgcctcc cttttttctt
    12001 agagtaaaca aaatcactta tctgagcaac tcataacatc taaaaagaaa aggttgtaaa
    12061 tgagacgtgc agctttaagc gataagaagc aattttgaaa aatagttttt cacaatttat
    12121 aaaattattc cttattgccg aaaggctggc cctttttatg agtatgccaa ttttttatat
    12181 cgaatgctca aaagatgagt gattttttgg ttgactatca tgtttgtgta ctgaataggt
    12241 ccagtagaac agtattttta cgacttgata attaagggat gaaattttca gaaatatgac
    12301 gtataatgta aaatactgaa aagttgcgac cttcaactcg tagatgtttc agaatctaat
    12361 tgaaatgatg gtgatatcat gacagacaac agcaaaacac gcgttgtcgt agggatgagc
    12421 ggcggtgtcg actcatctgt aacagcactt ttgttaaagg aacaaggata cgacgtgatc
    12481 ggaatattca tgaaaaattg ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa
    12541 gattataagg atgtagcgaa agtagctgct caaatcggga ttccttatta ctctgtgaat
    12601 tttgaaaaag aatattggga tcgtgtattt gaatatttcc tagccgaata ccgcgcagga
    12661 cgtacaccta acccagacgt gatgtgcaac aaagaaatca aattcaaagc attcttggat
    12721 tatgcaatgg atcttggcgc cgattatgta gctactggac attatgcgca agtcactcgc
    12781 gatgaaaacg gcgtggtcca catgcttcgt ggagtagaca ataataaaga tcaaacttac
    12841 ttccttagcc aattatcaca agaacagctt tcaaaaacga tgttccctct aggcggcatg
    12901 gaaaaatcag aagtacgggc aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa
    12961 gattcaacgg gaatctgctt tattggtgaa aagaacttca aacaattttt gagcaactat
    13021 ctgccagcaa aaaaaggaaa tatggtcact cttgatggtg aagtcaaagg acaacatgct
    13081 ggcttgatgt attatacgat tggtcaacgt caaggactag ggatcggtgg cggaggagat
    13141 tcgcaagaac cctggtttgt tgtaggaaaa gatctagcga caaacacatt atatgtgggg
    13201 caagggttcc atcatcctgc attgtatgcc acaagcttgg atgcaagtga aatccacttt
    13261 acgacgaatg aaccaatgcc aaaagaattc aagtgtacag cgaaattccg ttatcgtcag
    13321 caagacgttc ctgtgacagt tcgcttattg gatgataatc gtgcagaagt cgtattcgac
    13381 gaacctgtac gtgcaatcac tcctggacaa gcagttgtat tttatgatgg aatggaatgt
    13441 ctgggtggtg gtttgatcga ccacgcatat caagaaacaa aagttttaca atacgtttaa
    13501 gcgatattaa atgaatcaaa acagctatgg ctaacaaaag aaacaccttt tcttttggat
    13561 agcatagctg tttttttgca ataaaattaa tttttattca ttttatactt gaaatacttg
    13621 tgtaaaaagt tcataatatc caataatatg aataaagttg ctttttgtta agcaagagca
    13681 gttgcaaaat agaagggaat cttttgcaag tgatttgttc aattttataa gtgagggtga
    13741 tggaaatgga aaagaaatcg acgggtatta gcaaatgggg actggttgct ctggttgtca
    13801 gttcgtcaat cggaagcggt gtgtttggga tcacaagtga tctggctagt tctgcagctc
    13861 cagggccagc gatcctttca tggataatcg ttgggattgg tattttagct ttggtattat
    13921 cgctgaatca tttaggagaa aagcgtccag atctagatgg tggtattttc ggttacgcgg
    13981 aagcttcatt tggtaaatta ggtggcttca tcagtggttg gggttactgg ttgtctgcgt
    14041 ggctgggaaa tgttgctttt gcaacgatgc tgatgagcgc catgggagaa tttttcccga
    14101 tattcaaagg tggacaaaat gttccttcta ttttatttgc aagtattttt atttggggtc
    14161 tgactttatt agtaaacaat ggagttgaag gagcgagcgt catcaataca atcgtgacga
    14221 tctgtaaatt agtgccatta ttcctatttt taatatttgt atttatcgca tttaaagtga
    14281 atatattcac tgctgatttt tggggtaacg tatctgataa tctagtgaat ggaacagggc
    14341 agcaaggaac gatctggttg cagattaaag gttgtttgat ggttatgatg tgggtctttg
    14401 tagggatcga aggcgcaagt gttttagcca accgcgcgaa gaaacgttca gaagcccagc
    14461 aagcatcgat cattggtctg ctttgtttat tgtttatcta tatccttgct tcgatcttgc
    14521 catacggcgt cttatctcaa gaagagttag caactatttc acaacctgct atggcaaata
    14581 ttttaaaagg aatcgtggga aattggggag cagcgctgat caatatcggc ttgattattt
    14641 cgatcattgg ttcttggttg tcttggacga tgctcccagc agaaacaacg atgttgatgg
    14701 ctcgagacgg tctgttgccg aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt
    14761 attcgttagt tgttactgca atcttgacta atctattctt attgactttc ttagttactg
    14821 attatgccta tcaatttgcc tattctttat gtacagcggc aattttgatt tgttatctac
    14881 ttgtcgggat ctatcaagtc cagttttctt taaaagaagg aatcaaaaaa caagtagtga
    14941 tcggtgggat tgctgtagtt tttgaattga tgggcatcat tttagcagga ttttcttatg
    15001 tccttttatg tagtattgcc tatcttcctg gtttttgctt ctactggatc gcttgtcgtg
    15061 aaaccaaaca tcaaattacg ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg
    15121 cactgctttc aatcgggttg cttgctgtag gctggatcaa gatataaggg ggaaagaaac
    15181 atgaaaatcg caaatttcgg tgtagaagaa tggctaaata aatgggaaaa gaaagcggta
    15241 tatgatattg cacaaagctc gatcgaagca ttgacattag acgaactcgt tggcttagat
    15301 gggactagcg taagtaaatt tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg
    15361 atcgaaggat cagatgtttt caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg
    15421 gaaaacatct tacaaacgaa tggagcgact ggtgctaatc ttttagctct atacgcgtta
    15481 gtggaagcag gagaccatgt tgtttcgatg cttcctactt atcagcaatt atatgacatc
    15541 cccaaatctt taggtgccgc tgttgacttt gttcatttga aggaagaaga agattggcag
    15601 tttgatttag aacagctaga agccttagtc aaacctgaaa caaaaatcat ctgtttgaat
    15661 agtgcaaaca atcctacagg cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa
    15721 attgctcgta cagtagatgc atatgtattg atagatgaag tatatgcgcc tttaacggat
    15781 aaaggtgaat tcttgtcgat cgtagatgtc tatgaaaaag gtatagcaac caattcccta
    15841 tccaaaacct actccattcc aggtattcgt attggctgga cagctactgg acctgagttg
    15901 gcagaagttt ttcgaaagta tcgagactac acgatgattt gcggcggagt cctgtcagat
    15961 gatcttgctg tacatgcatt gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc
    16021 attacagaga accttgtgat tttaaaacaa tgggtagcca atgaaccgaa agttgaactt
    16081 gtcgctccca attacgtctc tacttctttc attaaattag ccatcgaaga agatgaccag
    16141 acattttgta tcaatttatt agaagaaaca ggagtactat tagtaccagg aagtgccttt
    16201 gatttaccga agcatgcccg actaggttac tgttgtaaaa aagaaacctt ggaaaaggga
    16261 ttattcctct tatctgagtt cttgaaaaaa caggcctaaa caaaaacact gcaaggacag
    16321 tctgtgacaa ctgttcaagc agtgtttttc atttacgctt attgtctatg agaaggagag
    16381 tgccctgaag ctatggagac tcctgaagct ttttcttctg cagtttcttg ctgatggatc
    16441 tcttgtgtat tgtgtcgtac aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga
    16501 aagacgccgc gataaccaaa tgcactggaa acactggagc cgatcatagg ccctactacg
    16561 ttaccagttg cttggaaaga ttggttataa ctgaagatcc gtccagcagc ttgatgaggc
    16621 gaatattttg tgatcaatgc ttggacagca gggagcagac aagcatcaga gataccaatc
    16681 aaaaaacgta aggcagctaa ttgccaaaca ttcgtgacga atgccattgg gatataaacc
    16741 agaatcgcaa aacccaaacc aatcgcaagt attctttcac tcccaatctt gtcacctaag
    16801 cgaccaaatc ttggagcggc tatcaaagtg gcaatcccag gtatcgaagc aatgattccg
    16861 ctgatcaacg taacattccc atgtccatgc agcaattggc gaatgtacag actgatgatt
    16921 ggactaatgg aattgttgga tgcttggatg atcatcgttg tgacgaacat accgatgacg
    16981 acgtgtggat atttcaagtc tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc
    17041 actgggacaa atttttcatg gacgaaaaac agactcaaca agaagacgaa aaataagatg
    17101 atccctgtaa tgaaaaaggt agggcgatag ccaaacgctg aagcagagat cccgccaaga
    17161 agcggtccaa gtaatgttcc tgtaacagaa ccagttgcta aggttccaag aacttgacca
    17221 cttttttctt ttggcgtccc agttgcgaca agggcagtgg cattactgat ataaccagaa
    17281 aaaatccctt gaagcagacg caatgcgact aattgataca cactagtcac gcaacccatc
    17341 aaagaaatga cgattgccat tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt
    17401 tggtcggcta attttcccca ccaaggagag ataagcgtag tcaccaagaa agtagatgaa
    17461 aaagtcaatc cgctccaaaa gttcaattgt gacgtactat aattacctaa cgtatctatg
    17521 taaagtgaca taaaaggcat gactaaacta aatccgatac cagccataaa tgttccaaac
    17581 cataagacaa acaagttttg ttcccaagtt tctcttttcc taaataattt ttcctttatt
    17641 cttgcagcca attagactcc ttctttctga aaattttcat ctacagctct ttattataaa
    17701 ctacaatatg tagtttatca atgaaagtct cttttttctc tttctttttc gtggtaaact
    17761 gaaagagtga aggatttatt ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc
    17821 tcaagagaaa atcatcgact gctttcgaga actagcagaa gagatggatg ttcagcaaat
    17881 aacctttcag catttagcca aacgcttgga tatcaaggct ccctcgctgt ataaccattt
    17941 caaaaatatt cgtgatgtca aaacagcgct aacagcgaaa cttctgcagg aacttaatga
    18001 ccagctgcga cgggctcttg tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga
    18061 aacttatcga tcctttgctt tttcaaatca agcagtgtat gagttactga tcagtgtacc
    18121 gcataccaat gaagctgttt tattagatgg aatccatgaa acgaatcaga tcattttgca
    18181 gatatttgag gcttttcaat taacgaaaga agaaaaagtt catcgaagca gggaacttcg
    18241 tagtatgatc cacggttatc tttccttacg ttttttagga tactttacaa aagaaccaac
    18301 aatcgatcca gaagacagct atttttggat gatcaataac tttatcgcta ctttgcctta
    18361 aacaatcaga ttcatttgta acaattttaa gatgttcatt aaatgaccct tttataagag
    18421 aaaattcttg caactttctt gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg
    18481 agcacgagta tgtatcaagt aattaccatg ttcggggaca atgagccttg gtggtttttt
    18541 gaagactggg aagaagatat tgaagaagaa gaaacattcg agtctttcga agaagcacga
    18601 caagcgtacg aaaaacaatg gaacgaaatc catcaaaatt atgaatatat caatgcaaaa
    18661 agtaactttc tcagcgcctt ttggaatgac ggagatgagc gctggtgtga agagtgtgac
    18721 gatgatttac agcaatacaa gggacttgcg ttgttgaaaa atcatcagcc catcacagta
    18781 gaaagcagaa aggaatttta tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa
    18841 agacctaagc aaggtgcttg gtgctaatca aaagcacaaa aattattatg aaggacccaa
    18901 ggtgatcgtt acttgggccc ttggtcattt attaggattg aaaatgccgg aagatctgaa
    18961 taaggaatgg cagagctggc aaatggacac gttgccaatg atccctaaaa aactaggaat
    19021 caaaccactt cctaaaacag gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa
    19081 agatgtctca gaagcagtca ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg
    19141 ctggatctta gagtatgtgc atttcaataa accagtgaag cggttatgga tctcttctca
    19201 aacagataaa gcaatcaaaa ctggattcaa gcaactaaga cctgcgaaag aatatgatgc
    19261 attgtatgaa tctgctcttg cccgggcaaa agcggactgg ctggtaggat tgaatgtgac
    19321 cagagcctta actgtgaagt acgcggataa tttatcagca ggccgtgtgc agacaccgac
    19381 actagcatta gtgagagacc aagaaaagaa aatcgagcag ttccgtccac aaacgtattt
    19441 cacgatcacg ttgcaagctg gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc
    19501 tttaaaaaca caagaagaag cacaaaaact tgttcaattg atcagtacaa gcaaaggaac
    19561 tgtcacagaa gtccaagaaa aattaaaaac ggaatctgcg ccattgcctt atgatttaac
    19621 agagatccaa agagaagcca atcaacgcta tggctattct gcaaagaaaa cattaagtct
    19681 tgtacaaagt ttgtatgaga cccataagat cgttacttat ccgcgtacag acagcaaata
    19741 tctgacaaat gacatgaaaa gtacgatgaa agaacgattg caagcagtca gcgactttgc
    19801 tcctgaggtc aagcagtatt tgaaaaacgg agccatcgtc caacagacaa aagtattcca
    19861 agacagtaaa gtgaccgatc accatgcact tttgccaact gaaaacagag cacgttatga
    19921 aaaattaagt aatgaagagc gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact
    19981 gtttgctcaa ccccataaag tcagccaaac aaaagttacg gttgaatttg gtaaagaacg
    20041 attcattttt cggcaaaatc gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga
    20101 gacggaaaca gtcaaatggg aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa
    20161 agaactatct gcaccgccta aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa
    20221 atacagtctg ggaaccccag ctacgcgtgc agagattatc gagaaactaa tcaaatctga
    20281 actgatggaa cgtacccctc aaggactgca agtatctcct aaaggtaagc aattattagt
    20341 attggtaaat ccctctttag taacaccaga tttgacagaa agctgggaaa aagaccttga
    20401 agctattgct gcaggaaaga aacaagcagg aaccttcttg aatggaatcg aaaaagaaac
    20461 aaaacgcttg gtgaatgaga tcaaatcaag caaacaagaa tatcaagatt tctcgattac
    20521 ccaaaagaaa tgcccagaat gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa
    20581 aatctatgtc tgcaccaatt cagattgcaa ctaccgcaga agaaaagatc caaaagtctc
    20641 taatcatcga tgcccgcaat gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc
    20701 ttatttccgt tgtaaatacg atggaacgac agaaaaattg cttgataaaa aagagcagaa
    20761 gaagaaaatg accaaacatg aagaacgacg cttgctgaaa aaatatgcac aagcagaaga
    20821 accagaagaa agcccattag cagcagcact aaaagcagca atgaagaagt aataagatat
    20881 gaaagaggtt gtgatagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg
    20941 cttctcaaat ttttgtgaaa tttcggctta tttccgaaga agctacttct ggaacaccat
    21001 ttattcggaa aaaaggactg tgacaagact tttgtcacag tcctttttca tttaaaaaaa
    21061 tgtttacttc atttgacgta tattattggt gaggcttcaa ttcatttggc ttcacttttt
    21121 ctgtttctct tatgatataa acaggacgct gtttcgtttc taagaagatt ttcccaatat
    21181 atttcccgat gattccaaga cataacaatt ggattccacc aatgaacagg aagatcgaga
    21241 ccatggaagg ccatcctgat gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca
    21301 aaattgcaat accagaaccg acacaagaca aagcgccgat aaatgaagca atcgttaacg
    21361 gtgcatccga gaagttcacg ataccatcaa tcgagtagtt gaataatttc cagaaggacc
    21421 aagatgtttc gccagccaca cgttcgcggt tttcataaga aatatattct gttttaaatc
    21481 cgacccagct aaataatcct tttgaaaatc ggttgtattc tgttaattct aggacggcat
    21541 ccaccatttg acgagtcatc aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc
    21601 tgattttatt gatcaactgg tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc
    21661 cttcacgagt cgaacgccgg gtgccgacac aatcgatatc agaagtagtg atccggttga
    21721 tcatctcagg aagcagttcg ggcggatctt gcagatcaac atccatcacg gtcactaaat
    21781 ctcctgtaga agcttgcaat ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa
    21841 atgagacaaa gcggacataa ggataagctg aggctagctt cttgattgct gctaacgtac
    21901 cgtcttttga tccgtcgtca ataaacaaat attcgaactc atgggcacct ttgaattttt
    21961 caacctcatc aaaaaaaaga ggaacagttt tttcttcatt aaaacatggg acaacaattg
    22021 atactttcat cgtttctatc ctttcaatcg tatgtttata tatggtttac attcaaaatt
    22081 taataaacct taaataacac ctttaaaatt tgattttacg taaaagaaca ggtattcttt
    22141 ttgtaaacta acgtatttag tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa
    22201 tttttattag ttggcaagta gttttggata gagaaaaagg aggaagttcc agtctattga
    22261 ctaggagcaa acaaaatgaa aatacacaca cctatcggct ttttatggtt agatgcagat
    22321 gaatccggat tgactgctgt atcttttcag ccaatttcag aaagcgaatc agcgaatcag
    22381 cgaattttaa cgcgcacaca agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa
    22441 tttgatgttc ctttatctat aaagagaggt acgccttttc agcaaaatgt ctggaaagct
    22501 ttgtcgacca ttccttatgg agaaatccga acgtataaag aagtagccgt tgctgttgat
    22561 agtccgaaag ctgtgcgagc gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt
    22621 attccttgtc atcgagtgat cgggcaaaat ggaaaactga caggatatat gggaaacgca
    22681 atagatgggg ttgaaatcaa acgccaattg ttgttgttag aaggatatct gcaaaattaa
    22741 aaacagttgt gattcggaca ttgttccatg cgagaatcac aactgttttt tcatgttcat
    22801 tctaaacttt gatcagccaa ataaatagta aagccatcac tgtttggaga agcccgacag
    22861 ctaatccata agataagaag cgtgtacctt ctttcatgaa ttttgcaaag tctaaacgca
    22921 gaccaattgc cgctaaagca gtgatctcaa accagctgct gataaaatgt gccgattggt
    22981 caataactac aggtaaagaa aacaagctgt tgaccaaaca aaagatcaca aaccctaata
    23041 cataccaagg aagcggtaat tttcgttttt tctgcgtatc agttggcgtt tcaaacagag
    23101 ctggttgttt cttgtgcatg aatttttcaa acagatagac aacaacaacg agcatcataa
    23161 tacgtagaat cttaaatagc atggcgaatt cgacaacaga attgtcaagt aagcttgccc
    23221 cagcgactac ttgaccaaca gattggagtg taccacccaa aagtgcactt ttttcaatga
    23281 gatttccacc aaacaactgt aatccaagaa aagggagagt caacatcatc acagtaccca
    23341 gtagattgac tagtgtgatg atctgtcctt tttcttcctc tttcgcatgg atggaagggg
    23401 cgattgaagc aatagcagaa gaaccacaaa cagcgtttcc tccagccatc atcaaagaag
    23461 aagtatcgct gaattgcagt tttttaccaa tcaagtaagc accgatgata gtgactgaca
    23521 tttgcagtaa cacaaaaaca attcctttta caccaatttg agcaatcgtt tgagctgtga
    23581 cagttgcacc tagtaaaaaa acagagaatt ctaatagttt gctttcagca aattttgtgc
    23641 cgattgctag aacaggctgt ttaaaaaaag tatttcctaa taaaataccc aataaaatag
    23701 caatagtcgc tccacctaac tgtggcagat aaatagcaag aagcctgctg aaggcagcga
    23761 tggcaaaaga aagcagtaaa ccgggtaaga tttcgcggac cgctgaatcc tttacagatt
    23821 ttattggatg tgacaatgat ttcatttgat cggttccttt cgtcaataaa tactaaaagc
    23881 atactcgaac atagtagcac taaaccagta ttttgacaaa agaacaagag gtcgtgacaa
    23941 aagtcttgta cgacctcttg tttcgaataa acggtgttcc agaagtagct tctacggaaa
    24001 taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    24061 aagctgacca cttcttttac gacctcttgg taaacggtgt tcaaaagctt gcatctgcgg
    24121 taataagccc aaccagtaca aaaacatgaa cagctgtttt tgtttggccg ttcttattac
    24181 ttgatgcaga acagcttttt cacaacctat tgaatcgatt attaagcgat ttttttagaa
    24241 acaaattttc catgtttgct atgttcttta attaagaaac gtacgtaatg agcaagaacg
    24301 tagaagacaa ctacacttgc aaacagacat tccgcaactt ttaagaaatc caatacgcta
    24361 ctatagatac ctaaactgtt gaagtaatga acagtagtaa atatagctgt tgcagagata
    24421 acaagcggga atgtaaaggc tgcatagctt ggataaaaac gaagcttgaa caaacgtgga
    24481 agcatagaca gcacaacgaa atacaatact tgagataaag cgaacaatgc tacgacaaga
    24541 taaaaattag gttcagcaaa gtttttaaga taacctgtta ggcaaagaga accaggtgct
    24601 gcaacgatcg taatcagcgg acgtgtcgga tgagccatat ttcttacgaa aaagacacgt
    24661 ttgatgatga tcggcaataa gaacgcatag cagatcaatg aaatccaaaa gaaaattttt
    24721 ccaataagtg ggaagaagtt cccggcagtt acagtgatga ccccaaatcc aacgtaaatg
    24781 ataaaccagc ttggataaat tgcttcaatt gaaagatcag ctttgataac atggtgataa
    24841 ttgaaataaa agacaaggat cacttggaag ataacagcgc acagccaaat gaatttgaca
    24901 attggtgcaa tactttcata tgaaacaaaa taagtacaaa taaccatgag tgacatcgta
    24961 aaagttggtg aaacggaggc gatgattgga tcatgcaacg tttgtttcgt gtgttcgaat
    25021 aagataaaca atttaacaaa aatgaatatc ataagaatca tagagatacc gccagttaga
    25081 ttgcctaaag caacatggtg atagtcttta aacagatttc caagggaagc taacccaagg
    25141 atcaacccgc agatcgggat cggaataatt ttcaaaaagt tcttcatata taatattctc
    25201 ctttttattt aatagatttt agtaccaaga tgaacgaata cattgtaaaa tgaataggaa
    25261 tgtatgatta cgagcaagca gacagctgat tgtcttcatc taagttacta gttggatcag
    25321 caactgtttt gttgtacatt tctattatag atggtttttt gtatatttaa atcaaatagt
    25381 ttttatagta attatcataa aaagttatgg aagtggtgcc atgtttaagt tattaacgac
    25441 ttttcgagca gtatatgaaa caagaaattt ttccagagca gcagaagtac tgtttttatc
    25501 tcaaccagca gtctcgaatc aaatcaaaca gttggagcgg gaattgaata tcgatctttt
    25561 tcaacgtaac ggaagaaaag agatgcttcc aactaagcaa gccgacttat tatatgaacg
    25621 gacactggtt ttgttagaag aatgggaaga aactcagcaa aaattgctca atgaacaaaa
    25681 tgaagaagaa gtatgtcgga tcgttgcttc ccacaccttt gctgtctatc ttttgcctca
    25741 gttgatgaaa catctgatca aaaggtttcc taaagtgcat tttgagatcg aaatcacgaa
    25801 ttctcatgaa tcattagaac aagtagcgaa acatgaatcc gattttggtt ttatcgaaaa
    25861 gccattagag acttccggcg tacaaagata ctctttgatg gaagaccagc tagtcatcgc
    25921 tggggaccca aaaagcaaat tgtggcttgt aagagaagca aactctggtg tttttcatta
    25981 cacgcagcgt tattttgaag agatgaatat ccaagataag aagatgatcg tgaaaagtaa
    26041 cgatgtaatt gttgcgttgc tgaaggaagg aatcggtcgc tcgattttgt ctaagcgctc
    26101 tgttccagaa aatgtgccaa tgactccgtt gaatttccat cgatattttt atttcatcca
    26161 acgaaccaat ctggtttctg atcagctgct agagattgcc gaagagatcc ttcgatacaa
    26221 tgaaagcaca gaaacttctc acagcgagga gaatctcagc tggtagataa ttgcatgtat
    26281 caaaaaaagt tctatgatac actaaagata agcagtaggc ttttgtactg catttgctca
    26341 agcaactgca caaacggaaa gttaattttt cccaaatgtc tatggaaaga ctttccatta
    26401 tctaactgga ccgctataat aatcagcagt atggttttta agaaaaggga tgaaacatgg
    26461 aaaaaaaatt aaaagcttcg aacgataatc gtattgatta tggcgtgatt ttaccagtct
    26521 ttctgttatg tttgattggt ctgctctcat tatatgtagc acttagccat gatccgaaca
    26581 atccagcagt cttgaaaggc gtagggatgc aagttgtctg gtatttagtt ggagcggcag
    26641 caatcgttgt catcatgcat ttgaattcca aatggatctg gaaactgact ccttaccttt
    26701 atggtttggg gttagtggta atgggactct tgctcaaatt ctatgatcag aatttagctg
    26761 cttcaacagg gtctaaaaac tggtttcgat tcggtgcgtt tactttccag ccagcagagt
    26821 tgatgaagat tgcgtacatc ttgatgatgg cactgatcgt aactaaacat aatacgcaag
    26881 taaaagaacg gacgatgaaa tcggactttt ggctgattgg gaagctcttg atcgtgacaa
    26941 taccggtttt agcattgatc atggcccaag atgactttgg aacgatgctt gtctttcttg
    27001 cgatctttgg cgggattttc ctgatgtctg gaatctcttg gcgcatcatc gcacctgtag
    27061 taatcttggc agtagtcgtt ggagcaggaa cgatcttctt ggttacgact gaaggcggaa
    27121 gagacttgct ttataaagtt ggattcaaat cttatcagtt tgcacgtatt gattcatggc
    27181 tggacccttt ccatgatacg tcaggaatga gttatcagcc ggcccaagga cttttggcaa
    27241 ttggtaccgg tggcttgttt ggtaaaggat tcaacgtaag caatatctat gtgcctgttc
    27301 gtgagtccga tatgattttt accgttatcg gtgaaaactt cggctttatc ggtggtgcat
    27361 tcgtcatttt cttgtatttt attttaatct atcgtatgat tcgggtgtgt ttcgatacga
    27421 ataatgaatt ttacgcctat atcgcaagcg gtttgatcat gatgctgctg ttccatgtct
    27481 ttgagaatat tggagcaaat atcggattac ttcctttaac agggatccca ttgcctttca
    27541 ttagtcaggg aggatcctcg attttaggga acatgatcgg tatcgggttg atattatcga
    27601 tgagatacca aaatgaagca cctgcaagat cttcaaggag gtaagaaaat tgtacacctt
    27661 ttactggtac ccgaaatgtt caacatgcaa aaaagccaaa gcgtggttag accagcatgg
    27721 cgttagttat caaaccgttg atatgatcaa agaagtaccg aaggcctcaa cgattgagac
    27781 ttggctagaa acgagtcaag taccaatccg tcgcctgttc aacacaagcg ggatgaaata
    27841 tcgggaactt ggactgaaag atcaagtagg cgagtttacg gcttcaaaag cgagccaagt
    27901 cttatcgaca gatggtatgc tgatcaagcg accactgatc ttacgagagg accagcttgt
    27961 tgcaattgga ttcaatgaaa aaatttatga aggagaactg atataaaatg gcaggcaagc
    28021 atttgaagaa aaaggacaac ctttggatct tatttaatgg aaaagaatac tgtgttggtt
    28081 tgacaaatga agcgcaagaa gaacttggag acatcacgtt tgccaatctg ccaaaagcag
    28141 gacaatcgct aaaaaaagat gaacctctga tcgaagttga agcggaaaaa gcggtcaatg
    28201 aatttgccag cccgctaacg ggtacagttt cttctgttaa tgataaaatc ggtgaagata
    28261 tcaatatctt gaatgattca gatgaaatga atgcgtggat cttaagtttc aaagatgttg
    28321 atccaaaaga atttgacgaa ttataaaatc agaaaatcac ttgaaaaaag ttgacactga
    28381 ttcttccagt tgctataatc ataagcaatc ctatatgaaa cgagaaagct ttgaaaagaa
    28441 gagtacatgt cacggacgtt tgaagagagc cctgtttgct gaaaatgggt aacgaaaatg
    28501 agatggaaga tggtcttaga gcagagtaag cgagcgtaag caaacttact acgggagtgc
    28561 ccgttacagc gcgacagtat acggaaagaa gctaagtcca agtactggaa gaggctgtta
    28621 ttgcgaagta acggtaaatc aaggtggtaa cacggaagtt caagctttcg tccttttgct
    28681 taaggcaaga gggcgaaggc ttttttgttt gaaaaattat aagagaaaga tgtgaatggc
    28741 atgtcattaa tccaactaac gaatgtcaaa aagacattca atgggaaaaa cggaaacatc
    28801 caagccgtca atgacgtttc actaaacgtt gaaaaagggg atatttatgg aatcgtcggc
    28861 tattctggcg caggaaaaag tacattagtc cgtctcctga atggacttga gcttcccacg
    28921 agcggcgaag tgatcgtaaa cgatcaagat attacaaaat taaaaaacaa agaattacgt
    28981 acattccgaa agaaaatcgg tatgatcttc cagcatttca acctgttatg gtcaagaact
    29041 gtacttgaaa atatccagct gccgctggaa ttagctggcg taccaaagag caaacgtaaa
    29101 gaacgggcag aagaactgtt gcgtttagtc gggttagaag gacgcggaca agcatatcct
    29161 tcacaacttt ccggcggaca gaaacaacgc gtcggtatcg cacgagcatt ggcaaatgat
    29221 cctgagatcc tgctttgtga tgaggctact agcgcattgg acccgcaaac aacagaagaa
    29281 gtattagact tattgttagc gatcaataag aaattgaatt tgaccatcgt cttgatcaca
    29341 catgaaatga atgtgatccg caaaatctgc aacaaagtcg ctgtgatgga gttggggaaa
    29401 gtcgtagaag aaggagacgt attgactgtc ttcagacaac cgaaaaaaga agtaaccaaa
    29461 cgctttgtac aacaagacat cgaaccagaa gctgattcga cagaactatt agctgaactc
    29521 atcaaagaaa atccaagcgg ccgtttggca actttgacct ttagcgaaag aaatgccaac
    29581 gagccagtca tttcccaagc gattcgacgc tataacatcg atgtcaacgt tgtgtatgga
    29641 aagatcaaac aagtgaaaga aggttccttt ggttcattga ctgtcttgat gactggtgaa
    29701 accaaagaat tagatcaagc agaagtgttc atcaaagaac aaggagtagg aattgaggtg
    29761 atccatcgtg gataaaagct tttcagaaac atatctagat tttaaacaaa tcaacccgga
    29821 aacgatgaat caggctatcc ttgatacact atatatgaca ttaatctcaa tggtcttagt
    29881 gacgatcatc ggattagttt tgggattgat cctttactct attggacgca aaaacaaacc
    29941 gtatgctcga atcatttatg gaatcgtatc gatcatcagt aatattttcc ggtcaacgcc
    30001 atttatgatc ctgatggttt tgatcattcc atttactaaa gcaatcgtag gcactatgct
    30061 aggtgcgcaa gcagccattc cagctcttgt tttatccgca gcaccatttt atgcccgtct
    30121 agtagagatt gcttttcgtg aagtcagccc tggtgtactg gaagcagcag atgcaatggg
    30181 agcaagttac tgggaaatca tctggaaggt cttgatacct gaaagtattc ccgcattgat
    30241 ttctggatta acagttacaa cgatctcgat gatcggattt actgcgatgg ccggcgcgat
    30301 cggcgcaggt ggacttggag gtcttgcttg gcaggaaggc taccagcgtg gtaatctaac
    30361 agtgacattc gttgcgactt taattatttt actaatcgta tttatcgttc aaggaatcgg
    30421 tgatttctta actaagaaaa cagacaaaag ataataggat atagggggat gtaggatatg
    30481 aaaaagaaaa tttttggatt tgcagcagcg cttttgctga ctgtaggatt agcagcatgc
    30541 ggtaacaaca gcgacagcaa agattcagca aataaagcag acgacacgac attgaaagtc
    30601 ggcgcttcac cgacaccgca cgcagagatt ttggaacatg tgaaaccatt attgaaagat
    30661 gaaggaatcg atcttgaaat cgtcaaattc gatgactatg tattaccgaa ccaatctttg
    30721 gaagaaggcg acattgatgc caattacttc cagcatattc cttatttgaa caaagaaatc
    30781 aaagaaaaag gatatgactt tgtcaatgcc ggtgctgtcc acatcgaacc aatgggcttg
    30841 tactctaaaa aagtcaaaga tgtttccgaa ttaaaagatg gtgcaacaat cattacttca
    30901 aattctgaat cagactgggg acgcatcatc acaattctac aagatgctgg cttagtcaaa
    30961 gtcaaagatg gagtagatct ggaaacagca acattcgatg atattgaaga aaaccctaag
    31021 aacttgaagt tcaaccatac gatcgaccca tcacttttag cttctacgta ccaaaatgat
    31081 gaaggtgacc tagttgcaat caacgcaaac ttcgcttacg gcgcagggtt gaatccatta
    31141 gaagatgccg tattgcttga aaaggacaac tctccttacg tgaatattat tgcgactcgt
    31201 aaaggtgatg aagacagtga caaaatcaaa aaactgattg aagtgcttca ccgcaaagaa
    31261 gtccaagaat ggatcacaga caaatggggc ggctctgtta aaccagtaaa ggctgatgca
    31321 aaataaggta tggaacaaaa aagatgagaa ataagtctgt aatggcttat ttctcatctt
    31381 ttttatacct attctaattt agaaatcaaa attgtttaat aataaataaa agatatttga
    31441 ttttagtaag gaaggtcatt gcgcttcatt ctcaattagt ctaaaatgag tgagaacgaa
    31501 agagaatttg ggggtataat gcatgtctgt cttagaaata aaaaatctac atgtgtcgat
    31561 cgaggaaaaa gaaatcctta aaggcgtaaa cctaacaatg aaaaccggag aaatccatgc
    31621 catcatggga cctaacggca caggaaaatc taccttatct gccgctatca tgggcaatcc
    31681 aaactatgaa gtaacagaag gcgaaatttt gtttgacggg aaaaatgttt tagacttgga
    31741 agtagatgaa cgtgcgcgac ttggcttatt tttagctatg caatatccaa gtgagatacc
    31801 aggtatcacg aatgcggaat tcatgcgtgc agcgatcaat gcgaaacgtg atgaaaacga
    31861 caaaatttct gttatggcat ttttgaaaaa gttagatgaa aaaatggctt tactaaatat
    31921 gccagaagaa atggctgaac gttatctgaa cgaaggtttt tctggcggag aaaagaaacg
    31981 gaatgagatc ctgcaattgt tgatgttaga acctacattt gcaattttag acgaaatcga
    32041 ttcaggtctg gatatcgatg ctttgaaagt cgttgctaaa ggagtaaatg aaatgcgcgg
    32101 agacaatttc ggtgcattga tcatcaccca ctatcaacga ttattgaact atatcacacc
    32161 tgatgtcgtt cacatcatga tggaaggccg agtagtcatg acagggaatg ctgacctagc
    32221 taaacgtctg gaagcagaag gctacgctgg tatcagtaaa gaattaggaa tcgactataa
    32281 agaagaagaa gcgtaagggg gagcaagaat gaaagaaaaa aactggctcg attatcttga
    32341 cgcggtaaat gatttttctt taagtaaagg cgaacctgat tggatgagaa ccttccgtca
    32401 agacgcacta gcgaaagcag atgaactgcc attgccgcat atcgatcggg tgaaattcca
    32461 tcgctggtcg ttatttgatg tgaaagaaac acaaaccatc agtgaaacag gcacgattcc
    32521 ggcatttgat gcaatgaaag acaatcctgt gcttgtccaa caaggttcat ggacgatttt
    32581 tgaacaactg cctgtggaat tagcagaaaa aggagtcatt tttaccgact tgttcactgc
    32641 aatgatcgaa tatccagaac tagtccaaga atattatatg aaaaaagcag taaacatgaa
    32701 cgaagaccaa ttgactgcac tccatgtagc ttttatgaac agcggtattt tcctgtatgt
    32761 gcctaaaaat gtcgtgatcg atgaaccact cgagtcctta ttcattcaag atggcgcatc
    32821 cgatgagcac ttcttcaagc atgtgttgat cgtggcagat gaacatagtg aattttctta
    32881 tctggaacgt ttccagacaa ccaaagaaca agtagcaaaa tcatcaggta atatcatcgt
    32941 ggaagtcatc gctaaagctg gttcaaaaat caaatattca gccgtagatc aattaggaga
    33001 aaatattact tcttatatga atcgccgtgg gcacatttta cgagatgctt ctgtcgactg
    33061 ggcaattggt gtcatgaatg acggtcatgt gatcgctgac tttgactcag atttagcagg
    33121 agaaggtgca cacgcagaag tcaaaatcgt ggcaatcagc tcgggcagac aaatccaagg
    33181 aatcgataca cgagtaacca ataaagcccc tcatacgatc gggcatattt tgcaacacgg
    33241 agtgatccga gaaaaaggaa cattgacctt taatggtatc ggccacatct tgaaaggggc
    33301 aaaaggtgca gatgcccaac aagaaagtcg tgtcttgatg ctttctgata aagcacgtgg
    33361 tgatgcgaat cctattcttt tgatcgatga aaacgaagta actgctggac atgctgccag
    33421 tgtaggacga gttgatccag aagaaatgta ctatctgatg agtagaggat tgcataaaga
    33481 agaagcagag cgcttagtga ttcgaggatt cctcggttct gtcttaacag caatccctgt
    33541 agaacaagtt cggaaagaat tagtcgaagt aatcgaaggg aagttgaatg gatgatcgat
    33601 gctaaaaagc tacgcaatga ttttcctatc ttatttcaag acgtcaatga tgaaccttta
    33661 gtttatctgg ataatgcagc gacgacccaa aagccaaaac aagtactcca ggcattagcg
    33721 tactattacg aacacgataa cgccaatgtt catcgcggtg tccatacgtt agcagaacgc
    33781 gcgacccatg attatgaaca agcaagagaa aaagtacggg cgttcatcca tgcaaaagaa
    33841 acagcagaag tgctgtttac tcgtggtacg acgacaagct tgaattgggt agccagaacc
    33901 ttcggcgacc gctatatcaa agcaggagac gagatagtgc tctcttacat ggaacatcat
    33961 tctaatgtta tcccgtggca gcagttggca aaacgaacag gtgctgtctt gaaatatatc
    34021 gaactaacag aagaaggctt cctggatatg gaggatgcag cgaaaaagat tacggacaaa
    34081 accaaaatcg tctccattgc tcatgtatcc aatgtcttag gtgtcttcaa tcctgttcaa
    34141 gaattagctg aacttgtcca tgaaaacggc ggggtcatcg tagtagatgg tgctcaatct
    34201 gccccgcaca tggccattga tgtccaagca ctagactgtg atttctttgc tttcagcgga
    34261 cacaaaatgt gcggaccaac aggtattggt gttttatatg gtaaacgaaa atggttagaa
    34321 gaaatggaac ctgttgaatt tggtggcgaa atgatcgatt ttgtcgaatt atacgacagt
    34381 acatggaaag aactaccttg gaaattcgaa gcagggacac caaatatcgc cggcgctatc
    34441 gcattaggcc atgcaatcga ttatttgcaa aaactcggta tggacaatat ccatcgatat
    34501 gaagaagaac tagccgctta tgtgttgcca aaattacaag caattgatgg attgactgtt
    34561 tatggtccac aagacccgaa ttttcggaca ggagtcgttg cattcaattt ggatgggtta
    34621 catccccatg atgtcgcgac agctttagac atggaagggg tagccgtcag agcaggtcat
    34681 cattgtgcgc aaccgctatt gaagtacctt gaagtatcag cgactgctcg cgcgagcttt
    34741 tatttctaca acacaacgca agacgccgac cgtttagttg aagcaatcat ggcaacaaag
    34801 gagtttttcc aacatgggac tatctaaatt agacaattta taccgtcaag tgatcttgga
    34861 tcattcttcc catccgcatc atcgtggaag cttgactgac tcgacaagca agatcgaatt
    34921 gaacaacccc acatgtggag atgtgatcga attgcaagtc gatgtaaaag acggaatcat
    34981 caaaaatatc gcctttgacg gcagtggctg ctccatcagt acggcaagtg cctcgatgat
    35041 gacagacgcc gtcatcggaa aatcaataga agaagcagaa catttaactg tagactttct
    35101 acagcttgtc caaggaaaag aagtcaaaga tcccgaccaa ttaggcgatg ctgctatgct
    35161 tggtggtgta gctaaatttc ccgctcggat caaatgtgcg acacttgcct ggaaagcact
    35221 aggaaaagca atggaaaacg atggtaccgc agctttccat cattaccaca aggaagagga
    35281 gtgagaacat gggcgtacca gaattagaag aatataaatt cgggtttcac gatgatgtcg
    35341 aaccggtttt cagtacagga gaaggcttga cagaagaagt gatccgtgag atgtcaagaa
    35401 tcaaaggcga accagaatgg atgcttgaat tccgtctgaa atctctagaa acatttaaca
    35461 aaatgccaat gcaaacatgg ggccccgacc tttcagacat cgatttcgat gccatcaaat
    35521 attaccaaaa accaagtgac aaacccgctc gtgactggga agacgtacca gaaaaaatca
    35581 aagatacatt tgaaaagatt ggtataccag aagctgaacg agcttatcta gccggtgctt
    35641 ccgcacaata tgaatcagaa gtcgtgtacc ataatatgaa agatgaattc gaaaagttag
    35701 gaatcgtctt tactgacact gactctgctt tgaaagaata ccctgaatta ttcaaagagt
    35761 acttttcaaa acttgtaccg ccaaccgaca acaaattagc agcgctgaat tcagccgtat
    35821 ggtcaggtgg gacctttatt tacgtgccaa aaggcgtcaa agtcgatgtt ccattacaaa
    35881 cgtacttccg gatcaatgcg gaaaacacgg ggcaattcga acgtacgctg atcattgttg
    35941 acgaaggagc aagcatccac tatgtcgaag gttgtacggc acctacttat tcaagtaaca
    36001 gcctgcatgc cgccatcgta gaaatcttta caagaaaaga tgcgtattgc cgttacacaa
    36061 cgatccaaaa ctggtcagac aatgtgtaca acttagtaac caaacgagca aaagcatacg
    36121 aaggcgcaac cgttgaatgg atcgacggaa acctaggtgc aaaaacaacg atgaaatacc
    36181 caagtgttta cttagatgga aaaggtgcac gaggcaccat gctctccatc gcctttgcag
    36241 gagcaaacca aatccaagat acgggtgcaa aaatgatcca caatgcacca aatacatcta
    36301 gctctatcgt ctctaaatca atttcaaaag acggcggaga agtaaactat cgaggacaag
    36361 taacctttgc caaaaacagt gcgggttcga tctctcatat cgaatgtgac acgatcatca
    36421 tggacgaatg gtcgaaatcc gatacgatcc cattcaatga gatccacaac agccaagtct
    36481 ctttagaaca cgaagccaaa gtctcaaaaa tctcagaaga acaactctac tatctgatga
    36541 gccgaggcct gaccgaatct gaagcaactg aaatgatcgt catgggcttt gttgaaccat
    36601 tcaccaaaga acttccaatg gaatatgcag tcgaactcaa tcgattgatc agttatgaga
    36661 tggaagggtc tgtaggttaa taactatttc attgctcgaa acgttgatat taaagcgttt
    36721 cgagcgtttt tttatttgta ttttaatggt tggctacgtt tttggctacg tttggaatta
    36781 ttcgcccata aattttgcaa atcggtcacc agttttctta acagattgtg gtgtgacatg
    36841 agcatagata ttcatggtag ttttaatatc tttatgacct aatcgttcct gtacttcttt
    36901 tatcgacgca ccactttcaa acaataagct acagtgggta tgcctaaatc catgtggagt
    36961 aatttgaggc tccatcggtg tctttttgta aatccagtct aaccaatcgt ttactacttg
    37021 ggggtagtac aacttgtttg tgtttgtagt gaaaagaaac tgttttggtg aattcgtgtt
    37081 atagccatat aagatcatta ttttcatttg ttcttgttgc caaatacgca gttgtttaag
    37141 tgtaaaatca tcaagctgaa tcgttcttga tgaattttta gttttaggta cttgtagaac
    37201 tacttgatta aactcgtctt gagcgatagt tttaccaatt gttattgttt tattgaaata
    37261 gtctatatct tcccattgaa gcgccaatgc ttcgctttta cgcattcctg tataagctaa
    37321 taaacggaaa aaagtgtaca gtttaactga attattttct ttagtattct gtaaaaacag
    37381 ttgtaattga tctttcgtgt aaaacttcaa gggttgttct tcttcaattt ttcttggaag
    37441 caatgtttta gacataggat tatccttaca gacttccatt gctactccaa atttaaatat
    37501 ttgagcagtt gtttttctca agaaaccata ttgttttaat gttttgtgcc attcattcac
    37561 ctgtttttgg caatagctaa cacttatttt atccaatcgt aattccccaa aagcaggtaa
    37621 tatatggttt ttgcaaaaac gttgagcaac ggcaacagaa gacggtttta ctcttgttct
    37681 gtagttatca atccataatt catacaactc ttgaaaagtt gtaacattga ttttaaactc
    37741 ttgtttttct ttaaagtcta caagtagtct attaagtgct gtttgcgcct cagcttttga
    37801 tttgaatctt ctacgctttg tacgtatatc ttttccagtc ttgggatctt tccctaaata
    37861 agtttcaaat ccccaatatt tttcgccatt ttgtaattta taactaaaaa atgttgccat
    37921 ttgattctct ccttacttca ttattgcagg tagagaagtt att
//
LOCUS       DAFGFZ010000012        36570 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000012 DAFGFZ010000000
VERSION     DAFGFZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.012"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LR726_000514"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LR726_000514"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL6215624.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LR726_000515"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LR726_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL6215625.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LR726_000516"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LR726_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL6215626.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LR726_000517"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LR726_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL6215627.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LR726_000518"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LR726_000518"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL6215628.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LR726_000519"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LR726_000519"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL6215629.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="LR726_000520"
     CDS             complement(8054..8524)
                     /locus_tag="LR726_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL6215630.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="LR726_000521"
     CDS             complement(8570..8995)
                     /locus_tag="LR726_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL6215631.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="LR726_000522"
     CDS             complement(9062..9331)
                     /locus_tag="LR726_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL6215632.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="LR726_000523"
     CDS             complement(9342..10937)
                     /locus_tag="LR726_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL6215633.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LR726_000524"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LR726_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL6215634.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="LR726_000525"
     CDS             complement(14490..15050)
                     /locus_tag="LR726_000525"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL6215635.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="LR726_000526"
     CDS             15403..16377
                     /locus_tag="LR726_000526"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL6215636.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="LR726_000527"
     CDS             16547..17947
                     /locus_tag="LR726_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL6215637.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="LR726_000528"
     CDS             complement(18025..18747)
                     /locus_tag="LR726_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6215638.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="LR726_000529"
     CDS             complement(18826..19332)
                     /locus_tag="LR726_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL6215639.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="LR726_000530"
     CDS             19455..21254
                     /locus_tag="LR726_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL6215640.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="LR726_000531"
     CDS             complement(21322..22896)
                     /locus_tag="LR726_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL6215641.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LR726_000532"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LR726_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL6215642.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LR726_000533"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LR726_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL6215643.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="LR726_000534"
     CDS             complement(24673..25311)
                     /locus_tag="LR726_000534"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL6215644.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="LR726_000535"
     CDS             complement(25312..25962)
                     /locus_tag="LR726_000535"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL6215645.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LR726_000536"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LR726_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL6215646.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LR726_000537"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LR726_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL6215647.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="LR726_000538"
     CDS             complement(29105..29845)
                     /locus_tag="LR726_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL6215648.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LR726_000539"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LR726_000539"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL6215649.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="LR726_000540"
     CDS             complement(31216..32154)
                     /locus_tag="LR726_000540"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL6215650.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="LR726_000541"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="LR726_000541"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL6215651.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LR726_000542"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LR726_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL6215652.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="LR726_000543"
     CDS             complement(35314..35619)
                     /locus_tag="LR726_000543"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL6215653.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LR726_000544"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LR726_000544"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL6215654.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7989 c         6708 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtag
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DAFGFZ010000013        34448 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000013 DAFGFZ010000000
VERSION     DAFGFZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.013"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            352..1041
                     /locus_tag="LR726_000545"
     CDS             352..1041
                     /locus_tag="LR726_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6215655.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="LR726_000546"
     CDS             1028..2236
                     /locus_tag="LR726_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL6215656.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="LR726_000547"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="LR726_000547"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL6215657.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="LR726_000548"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="LR726_000548"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL6215658.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="LR726_000549"
     CDS             complement(6339..7034)
                     /locus_tag="LR726_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL6215659.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="LR726_000550"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="LR726_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL6215660.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="LR726_000551"
     CDS             complement(8233..8424)
                     /locus_tag="LR726_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL6215661.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="LR726_000552"
     CDS             complement(8445..9335)
                     /locus_tag="LR726_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL6215662.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="LR726_000553"
     CDS             complement(9322..10089)
                     /locus_tag="LR726_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL6215663.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="LR726_000554"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="LR726_000554"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL6215664.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="LR726_000555"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="LR726_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL6215665.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="LR726_000556"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="LR726_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL6215666.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="LR726_000557"
     CDS             14660..15043
                     /locus_tag="LR726_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215667.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="LR726_000558"
     CDS             complement(15211..15969)
                     /locus_tag="LR726_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL6215668.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="LR726_000559"
     CDS             complement(15987..16805)
                     /locus_tag="LR726_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL6215669.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="LR726_000560"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="LR726_000560"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL6215670.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="LR726_000561"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="LR726_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL6215671.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="LR726_000562"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="LR726_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL6215672.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="LR726_000563"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="LR726_000563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL6215673.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="LR726_000564"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="LR726_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL6215674.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="LR726_000565"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="LR726_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL6215675.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="LR726_000566"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="LR726_000566"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL6215676.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="LR726_000567"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="LR726_000567"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL6215677.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="LR726_000568"
     CDS             26847..27146
                     /locus_tag="LR726_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL6215678.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="LR726_000569"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="LR726_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL6215679.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /locus_tag="LR726_000570"
     CDS             27734..27970
                     /locus_tag="LR726_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL6215680.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="LR726_000571"
     CDS             28113..30086
                     /locus_tag="LR726_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL6215681.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="LR726_000572"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="LR726_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL6215682.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="LR726_000573"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="LR726_000573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL6215683.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="LR726_000574"
     CDS             32965..34257
                     /locus_tag="LR726_000574"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL6215684.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10510 a         6702 c         6821 g        10415 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34441 tttccgaa
//
LOCUS       DAFGFZ010000014        33677 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000014 DAFGFZ010000000
VERSION     DAFGFZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.014"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            104..274
                     /locus_tag="LR726_000575"
     CDS             104..274
                     /locus_tag="LR726_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215685.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            494..1201
                     /locus_tag="LR726_000576"
     CDS             494..1201
                     /locus_tag="LR726_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6215686.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1260..1904)
                     /locus_tag="LR726_000577"
     CDS             complement(1260..1904)
                     /locus_tag="LR726_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL6215687.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2031..2318
                     /locus_tag="LR726_000578"
     CDS             2031..2318
                     /locus_tag="LR726_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215688.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2426..2746
                     /locus_tag="LR726_000579"
     CDS             2426..2746
                     /locus_tag="LR726_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6215689.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2827..4140
                     /locus_tag="LR726_000580"
     CDS             2827..4140
                     /locus_tag="LR726_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL6215690.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4137..4856
                     /locus_tag="LR726_000581"
     CDS             4137..4856
                     /locus_tag="LR726_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL6215691.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4984..5535
                     /gene="raiA"
                     /locus_tag="LR726_000582"
     CDS             4984..5535
                     /gene="raiA"
                     /locus_tag="LR726_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL6215692.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5757..8291
                     /gene="secA"
                     /locus_tag="LR726_000583"
     CDS             5757..8291
                     /gene="secA"
                     /locus_tag="LR726_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL6215693.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8417..9518
                     /gene="prfB"
                     /locus_tag="LR726_000584"
     CDS             join(8417..8488,8490..9518)
                     /gene="prfB"
                     /locus_tag="LR726_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL6215694.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9623..10309
                     /gene="ftsE"
                     /locus_tag="LR726_000585"
     CDS             9623..10309
                     /gene="ftsE"
                     /locus_tag="LR726_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL6215695.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10293..11186
                     /locus_tag="LR726_000586"
     CDS             10293..11186
                     /locus_tag="LR726_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215696.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11338..12786
                     /gene="cls"
                     /locus_tag="LR726_000587"
     CDS             11338..12786
                     /gene="cls"
                     /locus_tag="LR726_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL6215697.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13123..13980
                     /locus_tag="LR726_000588"
     CDS             13123..13980
                     /locus_tag="LR726_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL6215698.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14095..15015
                     /gene="pstC"
                     /locus_tag="LR726_000589"
     CDS             14095..15015
                     /gene="pstC"
                     /locus_tag="LR726_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL6215699.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15015..15899
                     /gene="pstA"
                     /locus_tag="LR726_000590"
     CDS             15015..15899
                     /gene="pstA"
                     /locus_tag="LR726_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL6215700.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15914..16720
                     /gene="pstB"
                     /locus_tag="LR726_000591"
     CDS             15914..16720
                     /gene="pstB"
                     /locus_tag="LR726_000591"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL6215701.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16736..17494
                     /locus_tag="LR726_000592"
     CDS             16736..17494
                     /locus_tag="LR726_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL6215702.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17507..18184
                     /gene="phoU"
                     /locus_tag="LR726_000593"
     CDS             17507..18184
                     /gene="phoU"
                     /locus_tag="LR726_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL6215703.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18407..19438
                     /locus_tag="LR726_000594"
     CDS             18407..19438
                     /locus_tag="LR726_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL6215704.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19435..20202
                     /locus_tag="LR726_000595"
     CDS             19435..20202
                     /locus_tag="LR726_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL6215705.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20302..21870
                     /gene="liaX"
                     /locus_tag="LR726_000596"
     CDS             20302..21870
                     /gene="liaX"
                     /locus_tag="LR726_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL6215706.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22167..22487
                     /locus_tag="LR726_000597"
     CDS             22167..22487
                     /locus_tag="LR726_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL6215707.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22487..22837
                     /locus_tag="LR726_000598"
     CDS             22487..22837
                     /locus_tag="LR726_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL6215708.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23004..23942
                     /locus_tag="LR726_000599"
     CDS             23004..23942
                     /locus_tag="LR726_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL6215709.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23957..24787
                     /locus_tag="LR726_000600"
     CDS             23957..24787
                     /locus_tag="LR726_000600"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL6215710.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24831..25856
                     /locus_tag="LR726_000601"
     CDS             24831..25856
                     /locus_tag="LR726_000601"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL6215711.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25871..26809
                     /gene="galU"
                     /locus_tag="LR726_000602"
     CDS             25871..26809
                     /gene="galU"
                     /locus_tag="LR726_000602"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL6215712.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            26934..27374
                     /locus_tag="LR726_000603"
     CDS             26934..27374
                     /locus_tag="LR726_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL6215713.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27377..27979
                     /locus_tag="LR726_000604"
     CDS             27377..27979
                     /locus_tag="LR726_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL6215714.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28042..29145)
                     /locus_tag="LR726_000605"
     CDS             complement(28042..29145)
                     /locus_tag="LR726_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL6215715.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29355..30356
                     /gene="ccpA"
                     /locus_tag="LR726_000606"
     CDS             29355..30356
                     /gene="ccpA"
                     /locus_tag="LR726_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL6215716.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30711..31106
                     /locus_tag="LR726_000607"
     CDS             30711..31106
                     /locus_tag="LR726_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215717.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31106..31357
                     /locus_tag="LR726_000608"
     CDS             31106..31357
                     /locus_tag="LR726_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215718.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31400..31795
                     /locus_tag="LR726_000609"
     CDS             31400..31795
                     /locus_tag="LR726_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215719.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            31792..33048
                     /locus_tag="LR726_000610"
     CDS             31792..33048
                     /locus_tag="LR726_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL6215720.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33060..33383
                     /locus_tag="LR726_000611"
     CDS             33060..33383
                     /locus_tag="LR726_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215721.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33376..33642
                     /locus_tag="LR726_000612"
     CDS             33376..33642
                     /locus_tag="LR726_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215722.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11283 a         5792 c         7246 g         9356 t
ORIGIN      
        1 cgttagttcg agagctcttt tttttgcttt gctaccttgt tccttgaaga ttctgctgaa
       61 ttaagataga gttaagttaa ctacaagaat tggagggatc cacatgaaat ggaaagaaaa
      121 agtagacgaa gcagctgaaa agttatatga tttggtcaaa tcagaaaaat acaatattga
      181 agtgaatatt ccgaaaaaag gcggaaaagc ggtccaggta aaatcaaaac gcccaacgaa
      241 tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa caaaagatcc ataagtcggc
      301 attttcatcg attttagggt cttttgttat tttctaggtt gaacatatct aaaaaattgt
      361 ttctgacaat aggagtaggg agaacgatgt gtgtctaaat cctctgttga tggatagaaa
      421 cctttatctt tttcagttgt gaatcaggta tataccagtt ataatagaag aaacgaaagg
      481 acgggtgtac gttatgtcga atcaattacc tgtatatata cagattcatg accaattaaa
      541 aaatgaaatc gaacagggta tatggaaagt cggagaccgt cttccttcag aaagagaatt
      601 agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa gcaattcaaa acttggcaga
      661 tgaaggaatc ttagaaagaa agatcggttc gggtacgtat gttgcgcgag aaaaagtaca
      721 agaaaaaatg tctggtgcaa caagttttac agaaattatg gaatcacaaa atcgagtacc
      781 gtctagtcgg acaatctctt atttcttgac ttcgccaagc tcaagtgaaa tggaaaaact
      841 aaacttgagt aaagatgaca cgatcttgcg aatggaacgt attcgtttcg cagatgatgt
      901 ccctatttgt tttgaagttg ccagtgttcc acaaaagctg atcgatggct acagcaaagc
      961 agagattact gaatcgttgt atcgcacgct agaagaaaaa ggcgggaaaa agatcggtcc
     1021 agcgaatcaa acgattactg ctgtgttggc ttctgagaaa attgctgact atttagaggt
     1081 aaaacgagga gatgcagttc ttcgtttgcg ccagatttct tattttgaag acggtacgcc
     1141 gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt gagttttatt tggaaaaata
     1201 aaaaacaggt gatccctgat tccttattag aaataaaaga gaatcagaat cacctgtttt
     1261 tatatatgat cagttatcag ttgctcgtga taagaaacag aaccctcttc aaattggact
     1321 tgtccgttca gcagctcaat gatggattct ttaaagtggt cagcactttt ttcatctaca
     1381 gcacagatga cgctaacttg atcagtatat tggatttctt ttataaaaaa atgttggagt
     1441 tcaagataat tctgtaattt tcctagttgc ggatacgtga tcatcacaga gatctcctgt
     1501 tgcaaggtac cttctacgat tccaatgtca gacaatgctt gagagaccgc atgagtgtat
     1561 gctcgtatta atccgcctgt gccgagtttc gttcctccaa aatatcgagt gactactgca
     1621 gctacattga tcagttgatt ttttttgagg acttccaaca tcggtacgcc cgccgttcca
     1681 cttggttctc catcatcgct gcttcgctgg atttcatttt tttctccaat gatgaaggcg
     1741 ctgcagttat ggttcgcttt ccaatgttct ttttttattt cttgaatgaa actttttgct
     1801 tggtcctctg attctattcg tctaagagaa cagataaagc gtgatttttt gatctcaatc
     1861 tcactttgtc cattttcttt tattgtacga tagcgctcga tcatcttgtc acctcttttt
     1921 ttcattttct ttattcagta taaataaagt tctaggttgt ttcaacactg taacggttgc
     1981 gcaattgctg tagtatgcga taataaagac aaaggcaggt ggatctgggg atgcaggaac
     2041 gaatgaaaaa gtacgatgca attacgcatt atttgaagaa caatggcggc tctcaagtaa
     2101 cgttgacctt tacccaattt gacgaactgc tgtttcccag taacggactt cccaaaacag
     2161 caagggaaag tacagattgg tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg
     2221 gctggataaa tgcaggctat gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt
     2281 tcaataaatt agtgaaatct agttggctat ttgattaaat aaaccgcgtt tagaaaacgt
     2341 tgacaaattt ctttcttttt atataataga gtaacagacg cgaagaaaac gtttgcatag
     2401 tttgttttaa aaaggagaga aaagtatggc taaaaaaaca atcatgcttg tatgttcggc
     2461 tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat
     2521 cgactcggat attttcgctg tgtccgcttc tgacgcagat aacaaccttg caaataaaga
     2581 cgtggatgtg ttgttattag gcccacaagt tcgctttatg aaagctgatt ttgagaaacg
     2641 tctggaacca aaaggcattc ctttagatgt catcaatatg gctgattatg gtatgatgaa
     2701 tggcgaaaaa gtgttagatc aagcaatcac gctcataggt gaataaatag aacagtataa
     2761 ctatcgaaca agcgatccaa aaaatagatc gcttgttttt tcgtatattt ttttagggcg
     2821 aagaatatga atcatttttg gggtagaagg cttctgacga gagatattga agcaatagac
     2881 tgtgaggagg taaatttacc aaattatgag aagatcgcag cagtggaacg tttgggcaac
     2941 tggattcttt gtcatcgctg tggaatgagg acgtccgtca ttgatggaca gcttgcagat
     3001 tacggttatt tttgtacaca ttgtctatat ttagggcgat gcgacactca acaagattta
     3061 tatttgtttg atcagcctga agcaaaacct cgagaagtcg tctttttttg gacaggacag
     3121 cttactgaaa aacaaacgga aatctcaaag agaatacttt gccattcaga aaaaagacat
     3181 catcttattt gggctgtaac aggtgcaggg aaaacagaga tgctttatcc tattttagtg
     3241 gaaactttaa aagctggagg aagagtagcg atttgtacac cgcgagtcga tgtttgtaat
     3301 gaattgtttt tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt gctccatggg
     3361 aatacagcaa cggcttatac ctttacttct tttgttatct gtacgattca tcagctatac
     3421 cgtttttatc ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt tccttataac
     3481 ggggatgcag gattagctca tgctgtgcaa acagcgatta aaccagacgg acgattcatc
     3541 tacttaagtg ccacacctga taaaaagtta ctaaaggaaa tcaagcagac gtttgacatc
     3601 cacaaacttg ctttacgctt tcatcggcgt ttgcttcctg aacccgtctt ggttttcatg
     3661 aataattggc gacaaaaatg caagaacaag aagaagataa aatctttggt gcggatcatt
     3721 catcaattaa ttaaagaaaa ccatatcctt ttattttgtc caagtatcac gatgatgcat
     3781 cgtttgaaag ccacacttca agaggctctt ccgagatatg agatcacaga tgtttcttca
     3841 agagacaatc agcgggcgga aaaagtccag aaaatgcgag aagcaaaata tgatatactg
     3901 ttaaccacga tgatccttga acgtggagtc acatttgaaa gaatatccgt tatcgtttta
     3961 ggagcggatc atccggtttt tactaaatct tctttggtac aaatcgcagg aagagccgat
     4021 cgtaaagggc cttatacgaa cagtcaagta tactttttct atgaagaaaa aactgcagct
     4081 attcgaaaag cttgtcagga aatcaaagaa atgaatgaag aagggaagaa gatgctgtga
     4141 ggtgcagttg ctgcagtaaa gatatcttcc aaaatttaac attcagcgag attattggtg
     4201 tttcgaaata cagattacct cagacttgtt cagtttgtcg cgcacaattt catttattgg
     4261 aaacaagagg ctgttctggc tgtcaaaaac caatccattt caatctagag agtacgaaaa
     4321 agaaagtaaa gtctattccc gtttactgca gtgactgtca agaatggaag aagaaatatc
     4381 cgacctatga ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc
     4441 tcaatcaata caagtttatg ggagatatcc gactagcagg cacctttgca tataaatgga
     4501 cgcagataaa aaaagaatac ccgacataca tctattgtcc aataccttta tctgaaaaaa
     4561 ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt gaaagcagca agaattcctt
     4621 ttcagatgct actcaaaaga gagaagcatc tgctggctca agcaaaaaac acgcgaaagg
     4681 aaagattgga gatgcctcaa ccttttgaac ttgcagtcac cttttctaag atacaaaata
     4741 aaaaaatatt gattattgat gatgtctata caacaggaca gactcttttc cacgcagcag
     4801 attgtttgca ggtcgcacag ccgaaaatca tccgaaccat ttctttggcg agatgaatta
     4861 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
     4921 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
     4981 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
     5041 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
     5101 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
     5161 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
     5221 ttggttgttg ataaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
     5281 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
     5341 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
     5401 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
     5461 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
     5521 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
     5581 agctgccgca agtttttttg agtttcctcc tcttttagat gaaatatgaa taaattatga
     5641 agttcttact ttaatacgct taagatattt caattatcta taactaatga taaaatgaaa
     5701 agtaaagtct acatagtctg gataatacac agaaggaaaa ggagattaca caacgcatgg
     5761 ccaatttgat tcgttcactt atcgaaaacg ataagaaaga actaaaacgt ttagatagca
     5821 ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc attaactgat gaacaattac
     5881 aggcaaaaac tgctgaattc aagggcagat accaaaaagg agaaacatta gatcaattac
     5941 ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg tgtattgggg ttatatccat
     6001 accacgtaca attgatgggt ggaatcgtgc ttcacgatgg aaatatccct gaaatgcgta
     6061 ctggtgaagg taaaaccttg accgcaacga tgccagtata tctaaatgct ctgactggcg
     6121 aaggggtaca cgtagttacc gtcaatgaat atttagcaac ccgtgactcg acggaaatgg
     6181 gcgaacttta taatttctta ggattaacag tggggttgaa tatcaactca aaatcttcgg
     6241 atgaaaaacg cgaggcttat aattgtgata tcacttatag tacgaataat gaattaggat
     6301 ttgactactt gagagacaat atggtcgttt accgccatca aatggtacaa cgtccgctga
     6361 attatgccat tgtcgatgaa gttgactcaa tcttgattga tgaagcgaga acgccgctga
     6421 tcatttcagg gcaagcagaa aaatccacag ctttatatac aagagcagac aacttcgtga
     6481 aacgtttgaa agaagaagaa gattataaaa tcgatgtaca atcaaaaaca atcagtttaa
     6541 ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt ggataactta tacgacattg
     6601 aaaatactgc attgacccat cacttggatc aagcattgag agccaactat atcatgcttt
     6661 tagatatcga ttatgttgtc caagacggaa aagtactgat tgttgaccag ttcactggtc
     6721 ggatcatgga tggacgccgt tattcagatg gacttcacca agcaatcgaa gctaaagaag
     6781 gcgtagagat cgaagacgaa acgaaaacca tggcgacgat cactttccag aactatttcc
     6841 gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa aacagaagaa gaagaattca
     6901 gagaaattta taacatccaa gtataccaga ttccgacaaa tcgtccggtt atccgtgatg
     6961 atcgtccaga tttactttac ccaacattac aaagcaagtt ccacgctgtc gtgcaagata
     7021 tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg aactgttgcg gttgaaacat
     7081 ctgagttatt atcagatatg ctgaatcaag aacgtattcc tcatgaagtg ctgaatgcga
     7141 aaaatcactt taaagaagca gagatcatca tgaatgctgg tcaaaaaggt gctgtgacga
     7201 ttgctaccaa catggctggt cggggaacag atatcaagtt aggacttggg gtaagagaac
     7261 ttggtggtct agcagttatc gggacagaac gacatgaatc tcgtcgtatc gataaccagc
     7321 ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc acaattctac ttgtcattgg
     7381 aagatgattt gatgaaacga ttcggttcag aacgaatcaa agctttctta gatcggatga
     7441 agatcggtga ggaagatgct gttatccaaa gcaagatgct gacgaaacaa gtagaatctg
     7501 cacaaaaacg tgtagaagga aataactatg acacacgtaa aaacgtctta caatacgatg
     7561 atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg acaagaagta atcatggaag
     7621 aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg gacaatcagt cgtgtagtag
     7681 atgcgcatac acaattagag aaagaaaatt ggaatttgga aagtatcatc gattttgccg
     7741 gcagtactct tgtccatgaa gatagtatta ctcttgcaga tatcgaagga aaaacatctc
     7801 aggagatcaa agagtatcta aacgaacgag cacaagaagt atttaatacc aaagcggctc
     7861 aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt cgtgatcttg cgtgttgtgg
     7921 actctaaatg gacagaccat attgatgcaa tggatcaatt acgtcagtca atcggcttac
     7981 gtgcttatgg acagaacaat ccattagtcg aatatcaaac agaaggctat aagatgtttg
     8041 aagatatggt aggcgccatc gagtatgaag tcactcgaat ctttatgaaa gctgagatcc
     8101 gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc ttctcactca gaagacggag
     8161 atgccaaaga aaatagtaat actagcgcga aaaaacagcc aatccatgtg aacaaggtcg
     8221 gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata taagaactgt catggaaaaa
     8281 atgcaaacta atttctgaca gcaggattgg tgagacaaat tcttttttta agaattcgtc
     8341 tcaccttctt tctgttattt gctgattctg ttagactaga tataatgaaa aaagtatagt
     8401 gaaagaagga aaaaacatgg aaatatccga aattcgaaat ctcctgacag aaatgcagga
     8461 aaaggtcaca agtttcagga ggtctctttg acttagatca gctggaagaa gacatcgcgg
     8521 aagcagaagc aaaaatggct gaacctggtt tttgggatga cagcgaagcc gcacaaaaag
     8581 tgatcagcga aaataacgct aataaagaaa cctatgacca attccattct cttgccgaag
     8641 aactagaaga aatcgaattg atggtggaga tgctgcaaga agaacctgat gcagcaatgc
     8701 aagaagaagc agaaacacgt atcaatgcat tagatgaaaa aatgaagacg tatgaattat
     8761 ctatgctgtt agacggacct tatgatcgaa acaatgcgat cgtcgaattg catccagggg
     8821 ctggcggaac ggagtcccaa gactggggca gcatgctttt acgtatgtat acacgttggg
     8881 cagagcagca tggctatcaa gtagaaacac tagattatca agcaggtgat gaagcaggga
     8941 tcaagagtgt gaccctgctg attaaaggac ataacgcttt tggttatcta aaatcagaaa
     9001 aaggtgttca tcgccttgta cggatttcgc cttttgactc agcgaaacga cgtcatactt
     9061 ctttttgttc ggtagatgtg atgccagagc ttgatgaaac gatcgatata gaaatcaatc
     9121 cagatgattt gaaaatcgat acgtaccgag caagtggtgc aggtggtcag cacatcaata
     9181 aaacagaatc tgctgtacgg atcactcact tgcctacagg aacagtagta gctagtcagg
     9241 cacaacgctc acaattgaaa aaccgtgagc aagcgatggg gatgttaaaa gctaagcttt
     9301 atcaattaga agtggaaaag aaagaacaag aagcagctgc acttcgagga gaacaaaaag
     9361 aaatcggctg gggttcacag atccgttctt atgttttcca tccctattct atggtaaaag
     9421 accatcggac aaacgtagag acaggaaatg tccaagcagt tatggacggt gaccttgatt
     9481 tatttattga cggctattta aaaatgcatc tacaataaga actcattgtt aaattgaaat
     9541 aaaattgtaa gattttgcaa gcatgttgaa acattgccat gctataatag ctaaagactg
     9601 aaaaagatat ggagagaacg ccatgattga aatgaaggat gtaatgaaga agtactcaaa
     9661 tggtacgacc gctatccgca atctctcgat tgtcatcgaa caaggagagt ttgtctatgt
     9721 ggttggtccg tctggagccg gtaaatctac attcatcaaa ttgatgtatc gagaagaaaa
     9781 agcttcaaaa ggaactttga acgtcgcagg tcatgatcta atagcaatca aaaaccgcga
     9841 agttccttat ctacgcagag aaatcggcgt ggtgttccaa gactataaat tattaccaag
     9901 aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt atcggcagaa aacctcgaga
     9961 tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt ttgaaacaca aagttcgagt
    10021 ctttcctagc gaattatccg gtggggaaca gcaacgtgtc tctattgctc gggcaatcgt
    10081 gaatacgcct aaagtactta tcgcggatga accgacagga aatctggatc cagaaaactc
    10141 atgggaaatc atgaaattat tagatcggat caatgcccaa ggaactactg tcgtgatggc
    10201 tacacataac agtacgatcg taaatacgat tcggcaccga gtaattgcta tcgaaaacgg
    10261 ccgcattatt cgagatcaag cggaagggga atatggatac gatgattaga acctttttcc
    10321 gccatttatt agaaagtatc aagagtttga aacgtaacgg ctggatgaca gttgcttcag
    10381 tcagtgcggt aacgatcacg ttgactttag tcggtgtatt catggcagtt atcatgaatg
    10441 caactaaatt agctcaggat atagaaggaa acgttgatgt atctgtattc gtagatatcg
    10501 gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt gaatgactta gatcatgtta
    10561 aaaaagttga attttcaagc aaagaacagg aattgaaaaa aatccaaaac gaaatgggtg
    10621 attcctggaa attgtttgaa ggtgacaata acccgttata tgatgtgtat gtagtaagtg
    10681 ctactgatcc ttcttataca aaacaagtag cagaagaagc agcagatttg aagaatgttt
    10741 ctcgttctga ttacggtgga gcttcctcag atcggatttt ccaaatcgct ggtgcagttc
    10801 ggacatgggg attagttgca gcggcattgc tgctgttcgt agcgatgttt ctcatttcga
    10861 acacgattcg gatcacgatt ttgtctagac agcgtgaaat tcaaatcatg cgtttagtcg
    10921 gtgcaaaaaa tggatatatt cgctggccgt ttttccttga aggtggctgg atcggtctga
    10981 tcggtgcgat tcttccgatt ttgatcatca catttggtta tgcatggttt ttccaattga
    11041 tcaatccatc attattacgt tcccattatt cattgattcg tccacaaaat atcgtttgga
    11101 aaatcaatct tttaatggtc ggcattggtg tgatcattgg ttcacttggc tcgatcattt
    11161 ctatgagacg ttttttgaaa ttctaatacc acaatataaa taaaatgaga aagttggaca
    11221 gtttcgttcc agctttcttt ttttattggc taggagaata gaaggaagcg gcttttttcc
    11281 ctttacttta aaaaacaact tgggtattct taagttggta gaagaagagg tgaagccgtg
    11341 aaaattatcg ttgacaattt ttttacgatc cttttgatta tgaatatttt gctttccttt
    11401 atcatcgttt ttcgagaaag aaaggaaact gctcaaacat gggcatggct gctcgttttg
    11461 atgttcattc cagttgtagg atttatttta tatatttttt taggaagagg gatttccaaa
    11521 gagaaaatct ttgatttgaa gatccaaggg aaaatcggta agaacctgga aattgaagaa
    11581 gacaaacagg cattgatgcg agggctttat cctcatccgc ctactggaca agtagatgtg
    11641 aaacaattga tctatatgct cacggtattt gaaagtacgc tctatacgac taaaaatgaa
    11701 atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc tgattgaaga tatcaagcag
    11761 gcagaagacc atatccactt ccagtattat atttatcgca gtgatgcatt aggtgaagaa
    11821 gttcgagatg cattgacaga ggcggcaaga cgaggggtaa aagtcagagt ccttcttgat
    11881 gcatggggtt ccacacaagt ttcttccagc ttttttcaga atttgaaaaa agcaggcgga
    11941 gaagtcgcat ttttctttcc tctatttgtt ccgtatatta atcctcggat caattatcgc
    12001 aatcatcgta aaatcgtagt tattgatggg aaaatcggat atacaggtgg gttcaatgtt
    12061 ggaaatgaat atctaggatt ggtaaagaaa tttggttatt ggcgagacaa tcatttgcgt
    12121 atttacggag aagcggtgta taccttgcaa aaccgttttt tgatggactg gaattctcag
    12181 catacaaaag aagtaggcta ttctcccaaa tttttcccgt ccattcacac tactggtgat
    12241 atagctgttc aaatcgtgac aagcggacca gataccgaac atgaacaaat caaaatgact
    12301 tatttgaaaa tgatttcgtt agccaaacgg gagatattga tccaaacgcc ttattacata
    12361 ccggatggat cgattcatga agcactgaag ttggctcttc tttcaggcgt aaaagttcat
    12421 atccaaatac ctaacaaacc agatcatctt ctcgtttact gggcaacgta ttctttcgct
    12481 gcagagctga tcgagtatgg agcaaggatc gaaacatatg agaatggatt tattcatgca
    12541 aagacgatga ttatcgatgg tggcattgtt tcagttggct cagcaaatat cgatgttcgt
    12601 tcatttcgct tggactttga ggtcaataca ctggtttatg atgaacgaat ggcttcacgt
    12661 gtaagaaaag ctttttttga agactcgaaa atttctacgc atctcacaaa agaaatgtat
    12721 gaaaaccgag gaataatcat caagatgaaa gaaggattag caagattgat ttcgccgctc
    12781 ctttaaaaca taaaaaggac atgagaacca tcaacagatg gagatctcgt gtccttttct
    12841 aacggggcca tttctgagag tacgtcagaa atggagaaag cgtctcgcat gagcgaaatt
    12901 aactttgggg tatctcttgt tttttgggga aagcaagaaa taagctaata tatgctatta
    12961 aaagagacac atttcaaagc cagtatagca tagaaacttt taatgagata gtttttttta
    13021 caattatgag aatttatttt gttatttaat gattttgatt cgttttcatc aagtaatgaa
    13081 aatgataaaa taaacatact ttaggattaa aggagatttc taatgaaaaa acttaaatta
    13141 ttcttaccaa taataggatt agccattctt ttatcaggct gttcaaagtg gattgatcga
    13201 ggagaatcca ttacggctgt tggctcttct gctttacagc ctttagtaga aacagttgct
    13261 gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg tacaaggggg cggaagcgga
    13321 actggtttgt ctcaagtaca gtcaggagca gtcgacatcg gaaactctga tttgtttgcg
    13381 gaagaaaaat cggggatcga tgcctcagca ttagtcgatc atcgagtagc agttgttggg
    13441 atcactccta ttgtgaacaa ggatattggc gtaaaagaca tcagtatgga aaacctgaaa
    13501 aaactattta caggcgagat caaaaactgg agtgaagtcg gtggaaaaga tcagccagta
    13561 gttatattga accgcgcttc aggaagcgga acccgtagca cgtttgaaaa atgggtattg
    13621 gacggagaaa cagctgttca ggcacaagaa caagattcaa gcggaatggt gcgccagatc
    13681 gttgccgaca cacccggtgc aatcagctat gttgcttttt cttatgtttc cgatgatgta
    13741 gatacgttga atgttaacgg tgtagaaccg acagatgaaa atgtgacaac gaacaaatgg
    13801 acgatttggg catatgagca tatgtacaca agagatcatc caaaaaaact gacaaaagaa
    13861 tttttagact atatcttatc tgatgaagta caagataaca tcgttggaga actaggttat
    13921 atccctgttt ctcatatgaa agtcgaacgt gattggcaag gaaatatcgt taaagagtaa
    13981 tctttacaca aaatttacaa acttagctaa acttttacat gttattaaaa ccaaattgat
    14041 gttcaatttg tacactgagt gagtatgtta aacctgatta agggggagtc atctgtggaa
    14101 gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa aaatggaaca gcgtggaaaa
    14161 ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg tattatctat tttttatttt
    14221 gtagctagta aaggattggc cacctttttt actgataaga tcaatgtatt tgattttctg
    14281 tttggtacag attggaatcc aagccaaatc ggagcaaatg ggaaaccaat ggtaggggca
    14341 ttgcctatga tcacaggttc gtttattgtg acctttttat ctgctatcgt tgccacgcca
    14401 tttgccattg gtgcagcagt attcatgacg gagatctctc ctaaaaacgg aacgaagatc
    14461 ttacagccgg tcatcgagct gcttgtaggt attccttctg tcgtttatgg ttttatcggt
    14521 ttatcagtta tcgtgccttt tgtcagaagt atttttggcg ggacagggtt tggtatctta
    14581 gctggtacat ttgtgttgtt cgttatgatc ttgccgactg tcacaacgat gactgttgat
    14641 gcattaaagg cagtaccacg gcactatcgg gaagcttcat tagcgcttgg tgccacaaga
    14701 tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc ccgggatttt aacagcagtc
    14761 gtttttggta tggctcgtgc gtttggtgaa gccttagcta tccaaatggt catcggtaat
    14821 gcagcattga tgccaacagg acttactact cctgcttcta cattgacgtc catcttaaca
    14881 atgggaatcg gaaatacaat catgggaaca gttgaaaata atgtgttatg gtcattagcg
    14941 ctgatcttgc tattgatgtc attattcttt aatatcgtga tccgtatgat tgggaagaaa
    15001 ggagagctga aataatgaac gcaaaacgct cagataaaat tgctacaggt gttttatata
    15061 tagtttcggg gatcatcgta ttgattttag cagctttact actttatatc ttagttcggg
    15121 gaataccgca catttcatgg tccttcttga ccgaaccttc caaagcctac caagaaggtg
    15181 gtggaatcgg gatccagcta tttaactcga tttatttgct tttgatcacg atgatcatca
    15241 gttttcctat ttctttaggt gcgggtatct atctttcaga atatgctgga aaaaactggc
    15301 taacagatgt gattcgcacg tcgattgaaa tcttgagctc gttgccttct gtcgttgtcg
    15361 gtttattcgg tttcttggtt ttcgttattc aattccagta tggtttctcg attatttctg
    15421 gtgcattggc tttgactgta ttcaacttgc ctcttttgac tagaacagtt gaggagtctt
    15481 tacaagcggt ccattacaca caacgagaag caggattggc cttaggtttg tcacgctggg
    15541 aaacagtgat gaaagtggtg gttccagaag cgacaccagg gattttgact ggtgtgatcc
    15601 taagttctgg aagaattttt ggtgaagcag ctgcattgat ctatacggct ggacaaagtg
    15661 cacctgcgtt agattttacg aactggaatc cattaagtgt ttcaagcccg atcagcattt
    15721 tccgtcaagc tgaaacatta gctgtccata tttggaagat caatacagaa ggcacgatgc
    15781 ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt gatcattgct gttttactgt
    15841 tcaacttcgg cgctcgtgcg attggcaaac gattataccg aaaaatgact tcagcctaag
    15901 gagaagaagc aaaatgaaaa aatataactg gaatgaaaca aatgtcatca ccatgccaaa
    15961 agagaaagaa gtagcactat atacggatga tatacatgtc tggtatggcg acaatgaagc
    16021 aatcaaagga gtggatctgg aatttgaaaa aaataagatc actgcactga ttggtccttc
    16081 cggctgcgga aaatctacgt atcttcgttc gttgaatcga atgaatgatg ggatcgctaa
    16141 tacgaaaatc actgggaaaa tcatgtatca aggcgtagat attaatgcgc ctcaagtgga
    16201 tgtttatgag atgcgcaaac ggattggcat ggtttttcaa cgtcctaatc cgttcagcaa
    16261 gtcgatttat gacaatatta catttgcttt gaaacagcac ggcgaaaaaa acaaaaagaa
    16321 attagatgaa atcgttgaaa caagcttgaa acaagctgcc ttgtgggacc aagtaaaaga
    16381 cagcttggac aaaagtgcct tggctttatc tggcgggcaa cagcaacgtt tatgcattgc
    16441 tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac gagccggcaa gtgctttgga
    16501 ccctatttcg acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag actatacgat
    16561 catcatcgtg acgcataaca tgcaacaagc agctcgtatc agcgactata ctgccttttt
    16621 ctatctagga aaagtaatgg aatatgacaa aactaacaag atatttacca gaccaaaaat
    16681 tcaggcaacc gaagattatg tgtccggtca ttttggttaa gacggaggcc tcaacatgac
    16741 aaaagaaatt atttcatcta aagatcttca tctgtattat ggaaagaaag aagcactaaa
    16801 agggatcgat ctaagtttca atcaagggga aatcactgcg atgatcggtc cttctggttg
    16861 cggaaaatcc acttatctgc gttctttgaa ccggatgaat gacctgattc cgaatgtaac
    16921 gatcaccgga agtgttttat ataaagacaa agacatctat ggtccaaaaa cagatgttgt
    16981 tgaactaaga aaagaaatcg gcatggtatt ccagcagcca aacccatttc ctttttctat
    17041 ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa aaagataaaa aagtccttga
    17101 tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg gatgatgtga aagacaaatt
    17161 gcataaaagt gctttatctt tatccggcgg gcagcagcag cgtgtatgta tcgcacgtgt
    17221 tttggcagtt aatccggaaa tcatcttatt ggatgaacca acaagtgcgc ttgatcctgt
    17281 ttctactggg aaaatcgaaa gtatgctgtt agaattaaga gagcggtata cgatgatcat
    17341 cgtgacccat aatatgtctc aagcttcacg tatttcagat aagacggcat tctttttaga
    17401 tggtcacttg atcgaataca acgatacgaa gaagatattc atgaatccag aaaaacaaga
    17461 gacagaagat tatatttcag gtcgatttgg ataaggagga aacggaatgc tacgtacaca
    17521 atttgaagaa gaattgctga atctccataa tcagttctac gaaatgggga tgatggtgag
    17581 cagcgccgtg cacaagtcag ttcgtgcata catcaaacac gataaaatct tagcccaaga
    17641 agttatcgaa aacgatataa atatcaataa catggaaaca cgcttggaga agaaaagttt
    17701 tgaaatgatc gcattgcaac agccagttac aactgatttg cgtatgatca ttacggtcat
    17761 gaaagctagc tcggatttag aacgtatggc cgatcatgcg gtgtctgttg ctaaatccac
    17821 gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa aaagagatct ccgatatgtc
    17881 cgattatgtg aaaaaaatgg tggacaacgt cctagttgct tatgtcaaaa cagacgaaaa
    17941 agacgcacgt acgatcgcca atatggatca aagagtcaat gactatttca atcgaatcta
    18001 taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca gtcatcagtg gaacagacta
    18061 cttgaatgtt gccagttatc tagaacggat cggagactat gtgacaaaca tctgtgaatg
    18121 gatcgtctac ttggctacag ggaaaatcac tgagttaaat acgaaccaca atgagatttt
    18181 ctaaactgaa gctgtgatta tgccgtttgt ttatgaaaag aggttgtgaa aaagctgttc
    18241 tacatcaagt aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg
    18301 gacttattac cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca
    18361 aaacatgtca caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat
    18421 tagcatcatc actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc
    18481 cgctttatat gaagtacctg tcatcgagat agaaaaagta cgaataaaag aggatcatca
    18541 attgattaca ggaaaactga tgaactcgga tcaaaaagga gaatcgattt atttatcggt
    18601 tccttatcaa aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt
    18661 ccaaatgcat ggagatgaat ggcagctagt agcaaaaaaa aatgatggct ggcttttctt
    18721 cttcacaagt atcttcattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc
    18781 tttaggaagt ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa
    18841 aacagaaggg atttctctat taaataaatc tatcctgttc gtcgttatag ggatgattat
    18901 tagcatattg gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt
    18961 tacgagtgtt tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca
    19021 aggactgcgc tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc
    19081 tagcgtactg attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac
    19141 attagaagaa ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg
    19201 aaggcaagtg ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat
    19261 cagtgggtca gttccatcgc ttcttttata tttaggaaat caatggtctt tcaaagaaac
    19321 gatagaacag catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt
    19381 attatcgatc cccatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat
    19441 gtacttatta tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa
    19501 ggtatgtata ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc
    19561 ggttatcatc agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct
    19621 gtggttatct tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt
    19681 atactgatct ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc
    19741 caaggtttta catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt
    19801 gattttagat tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat
    19861 gcgagtgtag caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct
    19921 ttttaccaat tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta
    19981 atgacacttc tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat
    20041 ttagacattc cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg
    20101 gctattctta ccacattgtc cgcactaatt gttcttcctt taacctgcgt gactacagca
    20161 tatctcttta caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc
    20221 tatgacattt ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt
    20281 ttgcttaagg aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa
    20341 ggaatccttt cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac
    20401 gaaaagcaaa tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagataag
    20461 gcaagtcaat tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat
    20521 ccaaaggaaa gagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca
    20581 actgaagcga ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat
    20641 aaggcagcac gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta
    20701 ggcgagttga cagaagaaga attagcacag cgtcaggcgt tagaggcaga aatcaaagag
    20761 ttagaagcta gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa
    20821 gatatccgta agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg
    20881 ccagatgatt ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca
    20941 gaagctggat cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca
    21001 gtgaatgaca acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg
    21061 aaatttgagc atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca
    21121 gcaaacggaa acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag
    21181 atcaaacttt acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag
    21241 attgaggtta atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac
    21301 cttgttttct acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc
    21361 aacatcatga ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac
    21421 atcatcgtgg accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc
    21481 gatattcgaa acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca
    21541 tttggtgcga cagcagaaaa tctttctgtg tctttagtaa atggtgatgt acgtcttacg
    21601 attaaagaag ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc
    21661 gctttaccag atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaat
    21721 agccgtctgt ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg
    21781 caattccgtc gtgtttcaga tgatgagatc gcgcaagtgc aactttcaac gacaacagga
    21841 agtatctatt taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga
    21901 gcaataagaa gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc
    21961 cgatagtcta tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg
    22021 agtcacaagg aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga
    22081 ccgagaattt tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat
    22141 ttaaaatagt agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa
    22201 atgttgtttt gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg
    22261 ttatccgtgt gctttatgta ttcctgagct tagttgcttt tggaagtcca attattttat
    22321 acattttatt agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata
    22381 acaatgcata ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac
    22441 gcaaagaagc ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca
    22501 acgtatttta gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt
    22561 ccatgtgagc agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat
    22621 gtttgtcaaa ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt
    22681 gtttacgttt gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga
    22741 acattttagt ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt
    22801 caatgtaatt gtcagcgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg
    22861 gcgcagtttt tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa
    22921 aacgtttttt ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact
    22981 ctaatagaaa tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa
    23041 aattatcttt ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg
    23101 gggacatctc acgcccagga ctagagttga ctggttattt taattattat tcccataatc
    23161 gtttacagct atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc
    23221 gtttgatcat catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag
    23281 gattaccagc tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc
    23341 gttctcccat ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc
    23401 ttgcccctcg aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga
    23461 ttcaaggtga cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggcc
    23521 atcgattgat cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg
    23581 gagaaccgcc taaaatcctg gaacatctga ttgaaatccg aggaatcggg atcattgatg
    23641 taatgaatct ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt
    23701 atctggaagc atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg
    23761 ttgaaatcgc ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg
    23821 ttgcaatcat cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg
    23881 caacgaaaac atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact
    23941 agcaaggagt gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat
    24001 tcctatctac tggtatgcat tgatcatcgt atcaggtatc attatcgcta tgtggcttag
    24061 cagcagagag gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg
    24121 gggattgcct atctccatta ttggcgctcg cttatattat gttttatttg atcttcccca
    24181 gtatatagct gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg
    24241 cggactgatt gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt
    24301 gtggactttt ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg
    24361 gggaaatttc atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag
    24421 tctgcattta ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc
    24481 aacatttctt tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg
    24541 aaagaaacca caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag
    24601 tttcgggcgt ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat
    24661 ccgtatttct caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg
    24721 gcgcagaaaa aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat
    24781 tatctgattt ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata
    24841 gagtagcggt tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa
    24901 acggacatga cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc
    24961 aacacacgaa tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca
    25021 ccaatatgga agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag
    25081 caatacgttc tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc
    25141 atgcaagtaa aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg
    25201 aaatcccagc agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag
    25261 aagtagcaaa acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa
    25321 aatatgtaca agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga
    25381 tcggtgtaga gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc
    25441 acggcttagg gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga
    25501 tcagccgttt aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggaa
    25561 taggcgatct agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact
    25621 tgttaggaca agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag
    25681 gtgtgaatac tacccaagca gcgatggaac tagcccaaat gatggaaatc gaaatgccca
    25741 tcactcaaac aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag
    25801 aaatcatgtt acgtgatgga aaaaaagaaa atgaattctc attaacaatt ttttgaagga
    25861 gcgaatgtat atgaaagtta aaaaagcagt tattccagca gcaggattag gaacaagatt
    25921 tttaccagca acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat
    25981 ccagttcatc gttgaagaag cgttgaaatc aggtatcgaa gacatcttga tcgttacagg
    26041 aaaagctaaa cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct
    26101 aagtgaaaaa ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt
    26161 gcatttcatc cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc
    26221 ctttattgga aatgaaccat ttgtcgttat gctaggcgat gatataatgg aagacgaagt
    26281 accattgaca cagcaattga tcgatgacta tgagcgtaca cacgcttcta cgattgccgt
    26341 tatgcaagtt cctcacgatg aaacttcaaa atacggaatc atcaacccag gggaagtact
    26401 ggaagatggg ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc
    26461 aagtgattta gctattattg ggcgttactt attgactcca gaaatctttg atgtattatc
    26521 agaacaacaa ccaggtgctg gaaatgagat ccaactaact gacgcaatcg atacgttgaa
    26581 taaaacacaa cgcgtatttg cccgtaaatt cacaggtaaa cgttatgacg ttggtgacaa
    26641 atttggtttt atgaaaacaa gtattgaata tggtctgaca catccagaag taggggcacc
    26701 tttgcgtgaa tacatcatgt ctttaggtaa aacattagaa gcagaaggaa gcaagatcaa
    26761 tcataatcca aaaggaataa cagaaatcaa agaagaaaag aaaaaataat ctttttttct
    26821 aacgagcttc agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa
    26881 aaacacgaaa atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag
    26941 gaggagaaat tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gttttcatcg
    27001 ttcgcgtatt gttgcaagtt tccaaaactt tagaaaaagt agaccagact gtcgcagaag
    27061 caaatacgac aattgagatc gtcacacaag acgtagatct tttatctaga caagtagaag
    27121 gcttgctagt caaaagtaat gagctgctct tggatatcaa tggaaaagtc gcaacgatcg
    27181 atccattgtt cacagcagtt gctgatttaa gtgaaagtgt atcggaactt aatcattcta
    27241 gtaaaaacat cgtgacgaaa gtcggcggaa tcggaaaaac gacagctaaa gcgactgttg
    27301 ccggaaaagt cggtggaaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga
    27361 caggaggaaa ataagaatgg ctaaaaaagg tggattttta ttaggtgcag tagttggagg
    27421 tacagccgca gcaatcgccg cattattgtt agcacccaaa tctggtaaag aacttcggga
    27481 tgaattggct gctcaagcag atgatttgat ggacatggct tcagattaca cagatatggc
    27541 aaaagaaaaa tcatctgagt taacaggtat tgcaaaagaa aaagcgcaag gtctttctca
    27601 acaagcaggg gatctagcag gcaatgttaa gcagaaggta agtgaatcag cagaggaagc
    27661 aggaagtgaa tcagatgata tcttgttaaa cctcaaagaa caatcagctg atttatctaa
    27721 tcgtttcaaa aaaacagcag aagatctgaa aaagccagca gacgaattag gtagtattgc
    27781 taaagatact tcagaagata tcatgatcga tgtaaaagat gctgcagata aagcaggaga
    27841 gacggtcaat gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgtt
    27901 gaaaatggca aaagaagatg caaaagaagc taaaaaagaa atgaaagata caatgacaga
    27961 ttcgactacc aatcactaag attactcatt tctagaataa aaaaggtgag agcggaccag
    28021 ctctcacctt ttttattttc attactctaa aacaagtaat tcttttggtg tagtcgtgaa
    28081 tggcacacaa ccatcacttg tcacatacac acaatcttcg atacgtaccc ctactttttc
    28141 aggaatatag attcctggtt caagagagaa gcacatccct tcttcaatca ctaagtcgtt
    28201 tccttctaca agagaaggga attcgtggac ggttgttccg attccgtgac ctaaacggtg
    28261 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
    28321 acctgctgtc acacctgggc gaactgcctc ttgtgcggca agctgtgctt caagagtgat
    28381 gttatagatt ttttcttgga actcagttgg tttttgataa gcaactgtac gagtagcatc
    28441 actgcaatag ccattccaaa taacgccaag atcgaataac actagttcat ttggtgaaac
    28501 cttcgtgctt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    28561 gtcgaaagac atttgtgaaa caccttgttt ttttaattgg tattcgattt ctgcgataat
    28621 tgcttgttca gttacgcctt cttttactgt tttgaagcca atttcaaagg caacatcggc
    28681 ccatttacca gcttcgatca gtttgtcttt ttcttctacc gttttgatca gttgcagacg
    28741 ctggataata gaagttagat cggtgtaaga atctgtacct gtcaagatat cagagatagc
    28801 atcgaagcga tcgacagtca gagcattctt ttcaatacct acattaccag gttgcccata
    28861 gcgtgcgcga agctcttttc cgatgatctc ccaaggattt tcgctgtcta agtagccacg
    28921 tacatcgtac gcccagctgc tgttttgagc atcttcaact tccagagcag gagtgaaaag
    28981 gaaaggatct tgttccagag ggataaaaag agctagtaca cgctcgtgag gatcactttc
    29041 atatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt ctacttggtg
    29101 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    29161 ctaaattaat tttgtattca ataattgtac cactgattga ttaaaagacc aacgttctta
    29221 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attgttgaga
    29281 ttatttgata tcctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    29341 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    29401 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa
    29461 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaat gcagtcgcac
    29521 gtggcttggc aagtaagaaa acaacaacag ttggggtaat cattcctgat gtcagcaata
    29581 tgttctttgc ctctttagca cgtggtatcg acgatgtggc aacaatgtac aaatacaata
    29641 tcatcttagc aaactcagat ggcaacgatc aaaaagaagt caatgtattg aataatttat
    29701 tggctaagca agtggacggc gtgatcttta tgggtcacca tatcacagac gaaatccgtg
    29761 gagaattttc acgttcaaaa acgcctgttg tactagctgg ttccattgat ccagatgaac
    29821 aagtaggtag cgtaaacatt gattatgtgt cagcaacaaa agatgcagta aataaattag
    29881 caaaaagcgg caataaaaaa atcgcttttg tcagcggtgc gctgattgat cctatcaatg
    29941 gtcaaaaccg tctaaaaggc tataaagaag cattaagcga aaacaatcta ccatatagcg
    30001 aaggattgat ttttgaggca caatacaact ttaaagatgg tctgtctcta gttgaccgga
    30061 tccataatag cggtgcaaca gctgtctatg tttcagacga tgaattagcc attggtatct
    30121 tggatggttt attagaccgc ggcgtaaaag taccagaaga attcgaaatc atcacaagca
    30181 acaattcttt actgacagaa gtagctcgtc ctcgtttgac aagtatcaca caaccattat
    30241 acgatttagg agcggtatcc atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    30301 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatcaact aaataataaa
    30361 agatcaatca tttaaaggtt gttccaaaag tggtcaactt ctggaacaac gtttatacga
    30421 gaaagggggt tatgacaaaa ttttgtcata accccttttt ttttgtgact ttaaaaaaag
    30481 agaaaatggc ggaaacaaac cttacgtcca tcgtatacga cacaatctgt gcgtagatgc
    30541 tacattttgt tcactactat caaaattact ttaaaatgaa aacgtgataa aaaagaaagt
    30601 agaaaagtag ataagtaact ctaaaaaatc caaaaaaatc caggctcgat tgcgtactta
    30661 tcttaaagga gaaacaatcc atcatatata acccgaaagg agacttctat ttgcctaaga
    30721 aaagacgaaa gaccatagtc atcattttga ccattcttac tataggaatc ggagggaaat
    30781 tttacatgga caaaagagaa atgcaaagac agaaagattt actcgcagta gaaaaacagt
    30841 ccgtcaaagt tctgaaaaat acctttgcgg atatcaagga agtgaagatt gaaaaatctg
    30901 ctcgaaatga aatgacgggt tcataccgga tagttattct aatgactaat aaacaagatc
    30961 aatcgattta tttttcttat agtttttgga aagaaagaaa tgagatagga agttacggaa
    31021 tagttgatga aaaaaagcaa aaagaaggaa atacgctcaa taaagttaaa gtaacttact
    31081 ctaatggaaa tgaggaaagt atttaatgat tactgacaaa caatatagtg aattaagtga
    31141 tgctgtttac tggctagatc ctaaacatag agactatgta cctgaaatgc aagaaaattt
    31201 aagttttaaa attaacggca ctatgtataa aatcctaaaa atcaaaaact ccttcgacgg
    31261 catgcaggcg atggctgtag cgccaatcgt ccatagcaaa ttggagaaaa atttcaaaaa
    31321 taaaaaaatt cctgcaaact ttcgcgtact taaataatga aggtgtcaat acttacataa
    31381 aaagaaagga ggcacgttct tgtcaaggaa aataagaaaa ctcagcttag ccattttatt
    31441 aatcgtcata ttagggatcg gagggaaagt ctatatggac aaaagagaag cacaaaggca
    31501 gaaagattta ctcgcagtag aaaaacagtc agtcaaagtt ctgaaaaata cctttgcgga
    31561 tatcagggaa gtgaaggtag aagaatttaa aaaaaaccct gttaccggaa gttatggtgc
    31621 acttgtgaca atgacaaata atgaagggaa atctgtttat tttgattata gtttttggaa
    31681 ggagagaaat gaacttggtg gttttggcat tgagaacaga gcggtacaaa agacaggagt
    31741 cactataaaa agagtaaaag taattttttc taacggacag gaggagttag tatgacaata
    31801 aacgataagc attacaatga tattagtgag aaggtttatt ggctagatcc caaatatccc
    31861 cgctacaatg agggatacaa aaagaactct gttaaagaat ttgctggtat ggaatttcaa
    31921 atcctacaaa tcaaagactc attggacggc atgcaggcga tgtctgtagc gccaatcaag
    31981 aacggtaaag tggatctttc tcaagtagtg attgcttttg caggaacgaa cttaagtgat
    32041 tggaaagatt tggaaacgga tgcaagaagt atcatgaatc tctacgggaa tagaccagga
    32101 gcaagttttt ctgaaaaagc aacaacaaac aagattttat ccgttagtgg attgaaaaca
    32161 gcggggcaaa tcaattcagc gattgcattt gctaatgaag taaaaaagga atatggagaa
    32221 gctgctattt ctactaccgg ccattcatta ggtggattct tagcattata tgtagcagca
    32281 gagaaacaat ggaaaaatgt cggcttcaac gggccggacc cgtatgatct cttatcacca
    32341 gaagcaaaaa aatgggtgga agaaaatccc ggtatgctca ccaattaccg aaatcacgcc
    32401 gattttatcg gtaattttgg tggtaatggc acaggtgctg aagttcggat cagtatggaa
    32461 acagcattct tgaatctgct tgcttcccac agtttaacct cttggaagtt tgatgaaaat
    32521 ggaaagctga ttattcctga aaatgaaaac aatgttaaag ctcgaaaaca acagaaagaa
    32581 aaccaagcga cccagttgtt ttcctatcag ttgcaagaac tgaacaacat acgtaaaaaa
    32641 ctacgattaa gtggcggccg gctgacagtt aatgaacaaa tttacttaga tgacacacaa
    32701 gctaggttag tagtggaaaa gttatcggca aaaatgaaaa gcgctatgga agaagttatc
    32761 cgcatcaatc aaagaggaat ttttgagcta gagaaaaaat ggcaagaagg cttgcggcaa
    32821 gcacgtgttg tagccccttc gttgactgaa caggaaatca tagaagcatt agctagtgtg
    32881 ggcgccacaa aagaaaactt agtcgattct gcgaaagaag tctatctgct acgaatcaat
    32941 aaggcaagac gtatggggga ggcctttgat acattggcga aggagatcca agacaaaatc
    33001 aatgaaatcg tggcacgaga tcgagaatta gcacagcaat tcaattaaag gagaagtaga
    33061 tgaaactaga agaattggaa agagattatc gaagtgaacg aagacaatta gaagctcaag
    33121 aagatgaatt gatccacttt aagaatcaag gattacgatt gatggagcaa gaggagcagc
    33181 gactgcatca tgtattaaag aacttagctt tagataatga gcctctgttt caagcaagac
    33241 gaagttattc gaattatcag gagcattatc aacaagccct acaagtagaa cgacgaaaac
    33301 tctatcagca gcaagatgaa ctagaacgtt cctatcgtca aaaaatcaga caaataacta
    33361 tggagaaatc atcttatgaa tgaattttta atgcaattac taaaagaatt agagaaaaat
    33421 ctacatcgat caaatgtacg ttcagttggt atggggatat ctcagcaaat ttgggcagaa
    33481 aaacgattga cagatcaaaa ctaccaaatg actgcagaag aagctgctca agaattttct
    33541 aaccgacatg tacacttgaa aggatcagta aaagggcaat ttgcagaaaa actaactgaa
    33601 ttattccaaa gacaagaaaa gaaattaaaa cagtttcgtt agtaaagtta tatagaaaaa
    33661 ttttgtgaaa atggttt
//
LOCUS       DAFGFZ010000015        33660 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000015 DAFGFZ010000000
VERSION     DAFGFZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.015"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..706
                     /locus_tag="LR726_000613"
     CDS             <1..706
                     /locus_tag="LR726_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6215723.1"
                     /translation="QSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(794..1186)
                     /gene="rpsI"
                     /locus_tag="LR726_000614"
     CDS             complement(794..1186)
                     /gene="rpsI"
                     /locus_tag="LR726_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL6215724.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1202..1645)
                     /gene="rplM"
                     /locus_tag="LR726_000615"
     CDS             complement(1202..1645)
                     /gene="rplM"
                     /locus_tag="LR726_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL6215725.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2164..2631)
                     /locus_tag="LR726_000616"
     CDS             complement(2164..2631)
                     /locus_tag="LR726_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL6215726.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2751..4289)
                     /locus_tag="LR726_000617"
     CDS             complement(2751..4289)
                     /locus_tag="LR726_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL6215727.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4369..5055
                     /locus_tag="LR726_000618"
     CDS             4369..5055
                     /locus_tag="LR726_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL6215728.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5256..5783)
                     /gene="msrA"
                     /locus_tag="LR726_000619"
     CDS             complement(5256..5783)
                     /gene="msrA"
                     /locus_tag="LR726_000619"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL6215729.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5867..6544)
                     /gene="pgmB"
                     /locus_tag="LR726_000620"
     CDS             complement(5867..6544)
                     /gene="pgmB"
                     /locus_tag="LR726_000620"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL6215730.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6541..7671)
                     /locus_tag="LR726_000621"
     CDS             complement(6541..7671)
                     /locus_tag="LR726_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL6215731.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7671..9854)
                     /locus_tag="LR726_000622"
     CDS             complement(7671..9854)
                     /locus_tag="LR726_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL6215732.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9851..10876)
                     /locus_tag="LR726_000623"
     CDS             complement(9851..10876)
                     /locus_tag="LR726_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL6215733.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10921..11748)
                     /locus_tag="LR726_000624"
     CDS             complement(10921..11748)
                     /locus_tag="LR726_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL6215734.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11745..12812)
                     /locus_tag="LR726_000625"
     CDS             complement(11745..12812)
                     /locus_tag="LR726_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL6215735.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12838..13674)
                     /locus_tag="LR726_000626"
     CDS             complement(12838..13674)
                     /locus_tag="LR726_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6215736.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13671..14540)
                     /locus_tag="LR726_000627"
     CDS             complement(13671..14540)
                     /locus_tag="LR726_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6215737.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14610..15893)
                     /locus_tag="LR726_000628"
     CDS             complement(14610..15893)
                     /locus_tag="LR726_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL6215738.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15907..17592)
                     /locus_tag="LR726_000629"
     CDS             complement(15907..17592)
                     /locus_tag="LR726_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL6215739.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17867..18892
                     /locus_tag="LR726_000630"
     CDS             17867..18892
                     /locus_tag="LR726_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6215740.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19487..23161)
                     /gene="rpoC"
                     /locus_tag="LR726_000631"
     CDS             complement(19487..23161)
                     /gene="rpoC"
                     /locus_tag="LR726_000631"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL6215741.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23219..26836)
                     /gene="rpoB"
                     /locus_tag="LR726_000632"
     CDS             complement(23219..26836)
                     /gene="rpoB"
                     /locus_tag="LR726_000632"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL6215742.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27564..28532
                     /locus_tag="LR726_000633"
     CDS             27564..28532
                     /locus_tag="LR726_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6215743.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28550..29455
                     /locus_tag="LR726_000634"
     CDS             28550..29455
                     /locus_tag="LR726_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215744.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29452..30258
                     /locus_tag="LR726_000635"
     CDS             29452..30258
                     /locus_tag="LR726_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6215745.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30394..31383
                     /locus_tag="LR726_000636"
     CDS             30394..31383
                     /locus_tag="LR726_000636"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL6215746.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31700..32224)
                     /locus_tag="LR726_000637"
     CDS             complement(31700..32224)
                     /locus_tag="LR726_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL6215747.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32511..>33660
                     /gene="scm"
                     /locus_tag="LR726_000638"
     CDS             32511..>33660
                     /gene="scm"
                     /locus_tag="LR726_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL6215748.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTT"
BASE COUNT         9521 a         7207 c         6324 g        10608 t
ORIGIN      
        1 tcaaagtatc actgcaccta aaaaaaatgc tttagaagaa aaaagaatac aagaaggtaa
       61 gcaagcacta tcatcaaaag aacttactaa ttggatagaa gcagtaaatg atgattttaa
      121 taatcattta cttacttttc atgattacac actttttatg attaccctgt atcttggtga
      181 tagaaaaagt gagacttacg ctttgcaatg gaaatatatt gattttgaaa aacaaacggt
      241 ccgtcttaaa catactttag ataaatatca aagaaaaaag tttacaaaag gtcgaaaaga
      301 taccgtcatt caagtaccag aagttgtaat gacattgcta agtgaatgga aatcggtaca
      361 agctgatcaa ttgttgaaat taaaaattaa gcaaacgtta gatcagtatc tctttactta
      421 tactaaacca tctggagaag taaattgtcc tgttcatgcc gattacctaa actatcgaat
      481 caattcaatt aaaagaaggc atcctgactt agttcattta tctccacaca aactccgtca
      541 tacttatgca actatagcta gacaaggtgg agcagatatg aatcaaattt caaatgcttt
      601 aacacactca gatatttcta caacaaagat atatgtgaac acaccagata tagttgataa
      661 ggctgttttt gaagcttttc agagaggatt gaataagtgt gattgatatg tgttcttaaa
      721 tgtgttctta aaaacaaaag aagcactttc caaataattg aaaagtgcca gaaacgttga
      781 tatataaagg atattaacgt tttgagaatt gtgaagcttt acgtgctttc ttaagacctg
      841 gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta
      901 aacggaagtc aggatcaact tctagcaatg cacgagcgat accatgacgg atagctcctg
      961 attgtccagc gtatcctccg ccgtttacgt taacaaatac gtcatatgcg ccttttgttt
     1021 cagtaacagc gaaaggttga ttgattactt cacgcaagtc agcatgtgga atgtattctt
     1081 caacatcttt tttatttaca gtaattttgc cagttcctgg tactagacgt acgcgagcaa
     1141 ctgcgttttt acgacggcct gtgccgatat attgaacttg tgccaatgaa attccctcct
     1201 attagattaa gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt ggatgttctg
     1261 ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca
     1321 taccttttac agaagtttcg atcaaacgac gagaattttt agcacgcaat tcgccagcag
     1381 tgattgattt caatcctcct gggtagttgc tgtgacggta ataaattttg tcagtcgctt
     1441 ttttacctgt taatttcact ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca
     1501 aatgaggtgt gaaagttggt ttatttttac cacgtagtac agatgcaaca actgttgaaa
     1561 gacgtcccaa tggaacatca gttgcgtcta ctacatacca tttacgttct acttcgcctg
     1621 gtttggccat atatgttgta cgcacgttat tttcctccaa tttaagttct gatgtttgac
     1681 atacacttcg agtttccggg gctctttgtg gggcaaacaa taccatttaa catattaccc
     1741 gctaatctag tcaatgtcaa cgtttttctg cttgattgaa aaatttatat gaaactttac
     1801 ttcttttctt tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg
     1861 ctgtgacaaa agtcttgtca caacccctta ttttgaataa acggtgttct cctgccaact
     1921 cctcggtcac aagtcacaat atccataaaa cataagaagc tgttttttaa aattgtttct
     1981 tgtgactcgg agctgaacgg caggcttaca acctcttggt aaacggtgtt caatcagctg
     2041 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct
     2101 ctaatactca ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag
     2161 tttttacgcg tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt
     2221 ttctgtggct gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc
     2281 agaaacttcg attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt
     2341 ttccaaacgt tctggaatag tacgatccca gcggccaatt tcatcaggga tttttgtgtt
     2401 atctgcaaat tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga
     2461 tacttcatct aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt
     2521 aaggaaacct ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct
     2581 tagatctgca actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc
     2641 ctcctaacat tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa
     2701 tcatttacct gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca
     2761 gaatcagcta attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt
     2821 tgtgtcgaaa caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact
     2881 ccaacttgct ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg
     2941 atgcttctcc agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa
     3001 acaagcgggt aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac
     3061 ccgcctgtag cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga
     3121 taaaggttga gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc
     3181 cgctgtaaac cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca
     3241 agtaaatagg gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa
     3301 agcagtgcat catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa
     3361 aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc
     3421 caatgtttct gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga
     3481 gtagtaataa aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca
     3541 aaagcaccta accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata
     3601 tcatttggca aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg
     3661 gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct
     3721 gcccattgga attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg
     3781 atcagttcgc caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt
     3841 tttttgaata attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt
     3901 ggatgaatcg gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt
     3961 ttttctgctt cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt
     4021 ttctcttcat ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg
     4081 attgtgtctg aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag
     4141 atcaattcct gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa
     4201 taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt
     4261 ccgatatcta atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa
     4321 aatttagttt aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat
     4381 tttatcattg gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta
     4441 ggtcactcta ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg
     4501 tatgaattga ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg
     4561 tgtcggttgc ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg
     4621 ttctttactt tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg
     4681 ctattttcgt tgatggattt attttacttc atggtccatt ctcacacgct attctgttct
     4741 tgggcaattt tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg
     4801 ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc
     4861 gatactctgt tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc
     4921 ttgtttcttg ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa
     4981 aaaaagttgg aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt
     5041 catcttaggt attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt
     5101 gttctcttac caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta
     5161 aatggtgttc aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg
     5221 atatacggtg ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga
     5281 cgaaggaaac ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc
     5341 ttgtaataat cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt
     5401 gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca
     5461 atatttcgtt cttcttcatc attatagtag atcactggac gataagaatc accgcgatca
     5521 gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga
     5581 tcataagaaa taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg
     5641 cctgttgtta cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc
     5701 acagaaatga tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct
     5761 gcgaaaactg ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc
     5821 caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata
     5881 cggcttctga taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca
     5941 catattcagc agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag
     6001 ccaaagctgc ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta
     6061 cattcaattg ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt
     6121 gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc
     6181 gtgcattttt gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa
     6241 gaacagctat tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat
     6301 atagattgtt tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca
     6361 tttttccaaa tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct
     6421 cgttttcttt ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt
     6481 aatgataacg agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt
     6541 tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt
     6601 gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag
     6661 gatcggaaaa acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact
     6721 tccgcaaata gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc
     6781 ttttcctttt agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg
     6841 ctgtacccgt ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt
     6901 ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc
     6961 ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc
     7021 taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc
     7081 gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg
     7141 cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt
     7201 ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc
     7261 gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg
     7321 tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg
     7381 gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc
     7441 gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa
     7501 ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag
     7561 atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc
     7621 taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc
     7681 ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt
     7741 aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac
     7801 ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga
     7861 gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc
     7921 catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct
     7981 aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg
     8041 caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca
     8101 tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac
     8161 agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa
     8221 tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc
     8281 ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat
     8341 aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta
     8401 acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat
     8461 tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca
     8521 gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa
     8581 aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg
     8641 atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat
     8701 gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca
     8761 ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac
     8821 agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt
     8881 tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca
     8941 ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc
     9001 gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct
     9061 gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg
     9121 gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc
     9181 tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga
     9241 ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta
     9301 ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt
     9361 tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct
     9421 acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta
     9481 ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg
     9541 agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact
     9601 ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct
     9661 gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca
     9721 aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat
     9781 ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt
     9841 tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca
     9901 ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga
     9961 gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct
    10021 tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag
    10081 cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa
    10141 acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat
    10201 cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac
    10261 caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca
    10321 tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt
    10381 tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt
    10441 aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac
    10501 gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc
    10561 ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga
    10621 gggcagccaa tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg
    10681 gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc
    10741 ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat
    10801 ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga
    10861 ttgctacttt cttcatttat ttctccctcc tttgcttatt tacttattgt tattctttct
    10921 ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg
    10981 caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat
    11041 ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc
    11101 agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat actctttttg
    11161 cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat
    11221 gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt
    11281 gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc
    11341 ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct
    11401 tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac
    11461 gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc
    11521 attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt
    11581 cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa
    11641 caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc
    11701 taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct
    11761 cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa
    11821 gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa
    11881 ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa
    11941 tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct
    12001 tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc
    12061 ccgccatagc cgatccctct taaagaagct tgtaatgcgg cagcactgcc actcgtttca
    12121 ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct
    12181 ttcataggat ctagcgtgta atcggcaccg tatttttctg ctaattctct acgctgcgct
    12241 atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt
    12301 tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc
    12361 cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc
    12421 aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga
    12481 atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt
    12541 tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt
    12601 tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata
    12661 aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt
    12721 actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct
    12781 attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta
    12841 tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat
    12901 aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc
    12961 tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag
    13021 gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga
    13081 aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag
    13141 accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag
    13201 catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt
    13261 atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga
    13321 aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt
    13381 tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga
    13441 gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc
    13501 ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc
    13561 ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa
    13621 tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag
    13681 tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa tagaatcgat
    13741 atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct
    13801 aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata
    13861 gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg
    13921 aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact
    13981 gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac
    14041 aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa
    14101 agctgcaacc tatctactaa cagatcattg acgattccat aactattatt gaacatatat
    14161 ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc
    14221 accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct
    14281 gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat
    14341 tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta
    14401 ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact
    14461 aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa
    14521 tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt
    14581 ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa
    14641 gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct
    14701 gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt
    14761 acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca
    14821 ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga
    14881 gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca
    14941 gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca
    15001 tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct
    15061 tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg
    15121 aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt
    15181 tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca
    15241 tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt
    15301 ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga
    15361 gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt
    15421 tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt
    15481 ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc
    15541 tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca
    15601 aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt
    15661 tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct
    15721 ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag
    15781 tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca
    15841 gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc
    15901 cacctcttat tttattattt tcagccacat atattcatac ggctggaata tcaactttcg
    15961 ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa
    16021 aagataacct gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc
    16081 ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg
    16141 atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc
    16201 tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg
    16261 attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag
    16321 cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc
    16381 taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata
    16441 cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg
    16501 ggtaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc
    16561 tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt
    16621 tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg
    16681 aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc
    16741 gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac
    16801 cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc
    16861 tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg
    16921 aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac
    16981 ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt
    17041 ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac
    17101 gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa
    17161 gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa
    17221 cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt
    17281 gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa
    17341 gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg
    17401 caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc
    17461 ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg
    17521 ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag
    17581 ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat
    17641 tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata
    17701 caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat
    17761 gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac
    17821 tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat
    17881 ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa
    17941 ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa
    18001 ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc
    18061 attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca
    18121 atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac
    18181 accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat
    18241 tattatttta agctctacga tggatgaaac acttcttttt gaattggatc aatacaatat
    18301 tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac
    18361 ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca
    18421 tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta
    18481 ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg
    18541 ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc
    18601 aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca
    18661 atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc
    18721 ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa
    18781 agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat
    18841 tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat
    18901 tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt caatcactca
    18961 tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga
    19021 tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact
    19081 atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt
    19141 atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat
    19201 gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa
    19261 taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt
    19321 tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat
    19381 gttgttaaga tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa
    19441 aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat
    19501 tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt
    19561 cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag
    19621 ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac
    19681 cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga
    19741 aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag
    19801 gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata
    19861 atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt
    19921 gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac
    19981 gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt
    20041 gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg
    20101 ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga
    20161 ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta
    20221 cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag
    20281 gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca
    20341 actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt
    20401 caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg
    20461 tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt
    20521 ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat
    20581 gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt
    20641 catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac
    20701 ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag
    20761 tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca
    20821 agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag
    20881 gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag
    20941 ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt
    21001 gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat
    21061 ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt
    21121 caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg
    21181 catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg
    21241 ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg
    21301 gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt
    21361 atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt
    21421 caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt
    21481 tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat
    21541 gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca
    21601 ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat
    21661 cttgggaagg agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta
    21721 acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt
    21781 ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc
    21841 gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc
    21901 ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt
    21961 cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa
    22021 gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt
    22081 ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt
    22141 tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt
    22201 tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg
    22261 catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca
    22321 agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca
    22381 agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa
    22441 taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt
    22501 ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt
    22561 cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg
    22621 cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt
    22681 cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat
    22741 atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca
    22801 tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat
    22861 gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa
    22921 cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag
    22981 gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa
    23041 tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca
    23101 aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca
    23161 tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt
    23221 agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca
    23281 cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca
    23341 aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg
    23401 tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata
    23461 cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt
    23521 atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat
    23581 aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa
    23641 attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg
    23701 catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca
    23761 cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg
    23821 ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg
    23881 gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt
    23941 gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc
    24001 catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc
    24061 cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac
    24121 gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca
    24181 catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac
    24241 gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc
    24301 cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga
    24361 tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg
    24421 tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga
    24481 tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt
    24541 tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt
    24601 gttccataga tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa
    24661 cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt
    24721 atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa
    24781 cgacaccgtc atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca
    24841 taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt
    24901 tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg
    24961 caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt
    25021 ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat
    25081 tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt
    25141 cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa
    25201 atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc
    25261 cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt
    25321 cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt
    25381 gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa
    25441 agaattcttt gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg
    25501 tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga
    25561 tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt
    25621 cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa
    25681 tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt
    25741 ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat
    25801 ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga
    25861 aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct
    25921 cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca
    25981 aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat
    26041 cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac
    26101 gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat
    26161 gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg
    26221 tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac
    26281 ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc caagctccac
    26341 ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga
    26401 aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga
    26461 tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga
    26521 tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg
    26581 catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca taatctacaa
    26641 attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc
    26701 taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta
    26761 attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta
    26821 ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc
    26881 aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct
    26941 tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct
    27001 acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca
    27061 tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc aacacttcct
    27121 ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt
    27181 atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt
    27241 tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac
    27301 ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg
    27361 acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa
    27421 aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc
    27481 aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc
    27541 ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc
    27601 tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac
    27661 aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag
    27721 gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt
    27781 atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag
    27841 aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa
    27901 caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca
    27961 catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa
    28021 aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata
    28081 acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag
    28141 cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga
    28201 aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg
    28261 cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag
    28321 ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggta
    28381 aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg
    28441 tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca
    28501 tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt
    28561 gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg
    28621 tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc
    28681 tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg
    28741 tcttctaggc tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa
    28801 attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac
    28861 atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg
    28921 ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc
    28981 tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg
    29041 tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa
    29101 gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct tgcttgcgca
    29161 aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc
    29221 tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt
    29281 cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga
    29341 accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca
    29401 gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac
    29461 gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat
    29521 gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga
    29581 aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag
    29641 ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa
    29701 tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa
    29761 gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa
    29821 ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat
    29881 cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg
    29941 atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac
    30001 cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc
    30061 actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc
    30121 atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca
    30181 gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa
    30241 gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc
    30301 ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac
    30361 tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca
    30421 acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag
    30481 ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa
    30541 aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta
    30601 ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa
    30661 acgatgttga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat
    30721 tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg
    30781 ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa
    30841 cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt
    30901 gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga
    30961 aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt
    31021 catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat
    31081 ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag
    31141 ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg
    31201 gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga
    31261 acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca
    31321 aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa
    31381 taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc
    31441 tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt
    31501 attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca
    31561 agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta
    31621 tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag
    31681 ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct
    31741 gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg
    31801 gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt
    31861 aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag
    31921 cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg
    31981 tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat
    32041 cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga
    32101 tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa
    32161 acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat
    32221 ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct aggataccta
    32281 cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt
    32341 tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga
    32401 acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag
    32461 gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa
    32521 gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg
    32581 tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta
    32641 actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg
    32701 tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg
    32761 gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt
    32821 ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg
    32881 gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg
    32941 atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg
    33001 agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta
    33061 aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt
    33121 tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc
    33181 cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt
    33241 ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag
    33301 atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca
    33361 gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc
    33421 aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc
    33481 ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag
    33541 atacatctac cacaagtgat agctcagatt catcaacttc aagcgacagt acagatacta
    33601 cctctaccac aagtgatagc acggatacat cagcttcaag cgacagtaca gatactacct
//
LOCUS       DAFGFZ010000016        33195 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000016 DAFGFZ010000000
VERSION     DAFGFZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.016"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            181..1062
                     /locus_tag="LR726_000639"
     CDS             181..1062
                     /locus_tag="LR726_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6215749.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1075..2685
                     /locus_tag="LR726_000640"
     CDS             1075..2685
                     /locus_tag="LR726_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL6215750.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2762..3421)
                     /locus_tag="LR726_000641"
     CDS             complement(2762..3421)
                     /locus_tag="LR726_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL6215751.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3704..4369)
                     /locus_tag="LR726_000642"
     CDS             complement(3704..4369)
                     /locus_tag="LR726_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL6215752.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4516..5121)
                     /gene="dhaL"
                     /locus_tag="LR726_000643"
     CDS             complement(4516..5121)
                     /gene="dhaL"
                     /locus_tag="LR726_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL6215753.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5149..6159)
                     /gene="dhaK"
                     /locus_tag="LR726_000644"
     CDS             complement(5149..6159)
                     /gene="dhaK"
                     /locus_tag="LR726_000644"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL6215754.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6134..6580)
                     /gene="dhaM"
                     /locus_tag="LR726_000645"
     CDS             complement(6134..6580)
                     /gene="dhaM"
                     /locus_tag="LR726_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL6215755.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6683..7003
                     /locus_tag="LR726_000646"
     CDS             6683..7003
                     /locus_tag="LR726_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL6215756.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7115..7984)
                     /locus_tag="LR726_000647"
     CDS             complement(7115..7984)
                     /locus_tag="LR726_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL6215757.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8080..9384)
                     /locus_tag="LR726_000648"
     CDS             complement(8080..9384)
                     /locus_tag="LR726_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL6215758.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9381..9890)
                     /locus_tag="LR726_000649"
     CDS             complement(9381..9890)
                     /locus_tag="LR726_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215759.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10065..12200)
                     /locus_tag="LR726_000650"
     CDS             complement(10065..12200)
                     /locus_tag="LR726_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6215760.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12283..13938)
                     /locus_tag="LR726_000651"
     CDS             complement(12283..13938)
                     /locus_tag="LR726_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL6215761.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13940..14254)
                     /locus_tag="LR726_000652"
     CDS             complement(13940..14254)
                     /locus_tag="LR726_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL6215762.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14424..14684
                     /locus_tag="LR726_000653"
     CDS             14424..14684
                     /locus_tag="LR726_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL6215763.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14698..15012
                     /locus_tag="LR726_000654"
     CDS             14698..15012
                     /locus_tag="LR726_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL6215764.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15742..16296)
                     /locus_tag="LR726_000655"
     CDS             complement(15742..16296)
                     /locus_tag="LR726_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL6215765.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16422..18351)
                     /locus_tag="LR726_000656"
                     /pseudo
     CDS             complement(16422..18351)
                     /locus_tag="LR726_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18657..19373)
                     /locus_tag="LR726_000657"
     CDS             complement(18657..19373)
                     /locus_tag="LR726_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL6215766.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19374..21197)
                     /gene="glpO"
                     /locus_tag="LR726_000658"
     CDS             complement(19374..21197)
                     /gene="glpO"
                     /locus_tag="LR726_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL6215767.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21211..22707)
                     /gene="glpK"
                     /locus_tag="LR726_000659"
     CDS             complement(21211..22707)
                     /gene="glpK"
                     /locus_tag="LR726_000659"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL6215768.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22843..24303)
                     /locus_tag="LR726_000660"
     CDS             complement(22843..24303)
                     /locus_tag="LR726_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215769.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24293..25588)
                     /locus_tag="LR726_000661"
     CDS             complement(24293..25588)
                     /locus_tag="LR726_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL6215770.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25920..26597)
                     /locus_tag="LR726_000662"
     CDS             complement(25920..26597)
                     /locus_tag="LR726_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL6215771.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26627..27718)
                     /locus_tag="LR726_000663"
     CDS             complement(26627..27718)
                     /locus_tag="LR726_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL6215772.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27819..28253)
                     /locus_tag="LR726_000664"
     CDS             complement(27819..28253)
                     /locus_tag="LR726_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL6215773.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28837..30294)
                     /gene="ascB"
                     /locus_tag="LR726_000665"
     CDS             complement(28837..30294)
                     /gene="ascB"
                     /locus_tag="LR726_000665"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL6215774.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30291..32186)
                     /locus_tag="LR726_000666"
     CDS             complement(30291..32186)
                     /locus_tag="LR726_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL6215775.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32244..>33195)
                     /locus_tag="LR726_000667"
     CDS             complement(32244..>33195)
                     /locus_tag="LR726_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL6215776.1"
                     /translation="ENRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFH
                     ETEFLLTHIKQMKTRMDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAES
                     AYFVLYLAEALNPFRKQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFK
                     NQSNKITEYDAILTTEQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKT
                     KLLDHYYKEENQIKIHKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEY
                     QLEIPLIHQQRKIKRVFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT         9249 a         7089 c         5764 g        11093 t
ORIGIN      
        1 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
       61 ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct
      121 ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt
      181 atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta
      241 aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt
      301 tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg
      361 gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct
      421 tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc
      481 ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc
      541 tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc
      601 ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt
      661 ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc
      721 aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc
      781 gtgatcatac gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg
      841 actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac
      901 ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct
      961 ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca
     1021 ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact
     1081 gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag
     1141 atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa
     1201 gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg
     1261 attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg
     1321 tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac
     1381 gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt
     1441 cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg
     1501 cttttagcaa tatcgatcag ctgtacgatg atttttttcc aagcggattc gatcactgca
     1561 gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt
     1621 gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg
     1681 tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg
     1741 atgtggaatc ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt
     1801 cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag
     1861 gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa
     1921 cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt
     1981 tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa
     2041 cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt
     2101 gaaaaaaact ttacaagcac gatcatggta gtcttgattt gtctagctgc tattttacca
     2161 attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc
     2221 gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt
     2281 gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg
     2341 aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct
     2401 gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt
     2461 aaacttgctt atatctactt agcttactcc tttgcactca attactttgg cggaattttg
     2521 gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac
     2581 gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc
     2641 cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac
     2701 aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta
     2761 tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta
     2821 cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga
     2881 taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt
     2941 tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag
     3001 ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa
     3061 gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg
     3121 ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc
     3181 cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt
     3241 gatacacttt catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa
     3301 gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat
     3361 cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca
     3421 taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca
     3481 actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga
     3541 gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt
     3601 cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag
     3661 ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg
     3721 cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa
     3781 cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca
     3841 tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt
     3901 ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt
     3961 tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt
     4021 cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa
     4081 ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac
     4141 tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat
     4201 caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat
     4261 attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg
     4321 cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc
     4381 tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa
     4441 aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc
     4501 gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc
     4561 actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg
     4621 tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt
     4681 taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc
     4741 gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc
     4801 taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa
     4861 tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa
     4921 tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc
     4981 tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc
     5041 actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat
     5101 ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat
     5161 cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt
     5221 tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac
     5281 attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat
     5341 caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta
     5401 atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc
     5461 tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag
     5521 ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt
     5581 cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt
     5641 tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc
     5701 agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc
     5761 aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata
     5821 attcttaacg atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat
     5881 ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc
     5941 ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac
     6001 ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc
     6061 tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat
     6121 gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt
     6181 gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag
     6241 gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt
     6301 ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg
     6361 attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc
     6421 cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac
     6481 catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc
     6541 tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata
     6601 taataaattt ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta
     6661 attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc
     6721 cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa
     6781 agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga
     6841 actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg
     6901 agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat
     6961 gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt
     7021 taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa
     7081 cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt
     7141 aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt
     7201 catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg
     7261 tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta
     7321 ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt
     7381 gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt
     7441 gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg
     7501 cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg
     7561 caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat
     7621 cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt
     7681 cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag
     7741 aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta
     7801 cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg
     7861 ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga
     7921 aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc
     7981 ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat
     8041 aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg
     8101 gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt
     8161 cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag
     8221 gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc
     8281 tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc
     8341 gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca
     8401 aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat
     8461 atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc
     8521 ttttgcccaa cgttttctgt gtcgatatag catcttcaac gtcacaggta cttccatgaa
     8581 aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat
     8641 actgatgtct tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt
     8701 tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt
     8761 tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt
     8821 ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg
     8881 aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt
     8941 gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc
     9001 tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa
     9061 tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata
     9121 attcaatttc gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc
     9181 tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg
     9241 cttatgtttg tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa
     9301 aatcggataa gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt
     9361 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
     9421 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
     9481 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
     9541 aatttttcat cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc
     9601 cacctagtca atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg
     9661 attaagaaat acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct
     9721 ctttctggtc tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat
     9781 cctaaaagag ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac
     9841 ttatctttca ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct
     9901 cacgtttttt tgttacgacg taggagttta ccatacattt catttctttt cacataaagt
     9961 gttcctaaat ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat
    10021 tttgttcttt ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc
    10081 aatacgacaa tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt
    10141 tgctcatctc catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt
    10201 gctagactca aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat
    10261 tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt
    10321 agagaaacca aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg
    10381 tattcgggtt cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca
    10441 gcataggttt tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca
    10501 taccgatgga tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg
    10561 gaagaattaa agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat
    10621 aattcgatca atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga
    10681 tagcgaaatg ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg
    10741 actgcttcat caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca
    10801 taaattcttt caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt
    10861 atttttcgat attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa
    10921 tcatcagaaa tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc
    10981 tgattctgca cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc
    11041 agtgcctttt tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca
    11101 tatatttttt cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg
    11161 ataaaattcg gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt
    11221 ttgctacgca aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa
    11281 gcctcttcac cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga
    11341 ttcagtggat ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga
    11401 tgattaggtg atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc
    11461 agatacgcaa tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc
    11521 aagatatttt gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca
    11581 gtgatagcct tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct
    11641 tgaatagcta cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt
    11701 cgtctttctt taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca
    11761 atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca
    11821 tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc
    11881 agaaagtcta caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct
    11941 agctgatttt ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt
    12001 tcaatcatcg aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg
    12061 ccagccattt ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc
    12121 agttcgtttt ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctagc
    12181 tgtttttgtg tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag
    12241 tttacctctt ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat
    12301 cagttcttca ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg
    12361 gtagccagac tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct
    12421 gacgatatat tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg
    12481 gatatgtttg gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa
    12541 taaaattttt ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag
    12601 cccttctgca atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc
    12661 aaatggcggt gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt
    12721 gattgctgtt gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc
    12781 accataaatc tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc
    12841 tggataatag cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt
    12901 ttctttggct gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat
    12961 aattgctgtt ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac
    13021 ttgtctacct tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat
    13081 ttgctgtttt acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata
    13141 acgttcgtct actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag
    13201 ctggttatca ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc
    13261 atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat
    13321 cacttctact ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag
    13381 tgttggaaga atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag
    13441 gttttctgcc atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg
    13501 tttttctaat gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt
    13561 gtcggcttct gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac
    13621 caatgcatcg tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt
    13681 ttcgggaaag atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga
    13741 taatgattct ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata
    13801 ataaggcaag ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc
    13861 tgcatcttcc attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc
    13921 gacaataacg attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc
    13981 caccacgtac attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac
    14041 tcctcatccc tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt
    14101 ttatactttg taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag
    14161 gtgttctaac atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg
    14221 ttatcgattt tattgattga aatagcaaat tcattttctg cacctcattt acatttttat
    14281 aaatacctca tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct
    14341 tcattttttt cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt
    14401 ataaaaatag aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga
    14461 aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta
    14521 tggatgtcat cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga
    14581 tcgttgcaga taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag
    14641 cggctaaact agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg
    14701 aaagtcgttt tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa
    14761 aacctgataa aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt
    14821 accggttatc tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac
    14881 gcttgcgcca atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta
    14941 atcgttgtac cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat
    15001 atcaagcctt agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt
    15061 atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct
    15121 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg
    15181 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg
    15241 gtttgaccta agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt
    15301 tgagctaata ccgaaggtca gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt
    15361 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg atttctacat ttttgcataa
    15421 atttggctta ataccgagaa actggctttt gaataccgtt taaggaagag gttgtaaaat
    15481 cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagctgctca tattttgccg
    15541 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc
    15601 ctaccattta actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt
    15661 tcggttggct tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc
    15721 gacataggtc acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata
    15781 caagacaaaa gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag
    15841 cgttggtaat tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc
    15901 tactttgcca taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc
    15961 agctacaaac ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg
    16021 tcgataccat gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc
    16081 aagcaccttc cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata
    16141 atcagacaaa cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac
    16201 aaaataaaga taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt
    16261 ttgtatttct ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac
    16321 tcttttcttt ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt
    16381 tttctttaca gacactgata ttatgagcta atttgagata atcacttgct gttttcatcg
    16441 tttactcttc tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt
    16501 tttgatcctc gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg
    16561 tctaaatcgt aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga
    16621 cgaggcacgt ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct
    16681 tcataaacta acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag
    16741 agctgacgtt cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct
    16801 tttttctgtg tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc
    16861 atcgacatat ccattttatg agcaatccac aaagcttggc tttctcctgt ttcttccaac
    16921 ccgcaactga tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa
    16981 atcttcatga tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa
    17041 tgccccttgc tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg
    17101 ctctgttcca ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa
    17161 aatagcagcg atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg
    17221 gtggtcaccg acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc
    17281 cggaacttgc ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac
    17341 tgttttgcct ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc
    17401 aacatccaat ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat
    17461 atgttccgat ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg
    17521 agcaaagatg atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt
    17581 ttctgcgata gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc
    17641 ttttaacaac tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt
    17701 cgttctttta tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt
    17761 gataggaatc gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt
    17821 gtttttcgga tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg
    17881 taattgtttg cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt
    17941 tattttctgg tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc
    18001 aaacaacaag ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct
    18061 tctaagaaaa tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac
    18121 ttgttctgtc agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc
    18181 taagatcaag cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt
    18241 cgagaccgtc atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat
    18301 ttcctttttg atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc
    18361 ttttcttgaa gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata
    18421 gaacacagct taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct
    18481 gacaggtacc gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact
    18541 gattagatac cagctacact cacaaaacat accccattct tctaggtctt atttattttc
    18601 cacttaacta tagcaagaca cattttggtt gtcaaacttt agtatgtttt atagatttac
    18661 accattaatg aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt
    18721 acccatgaat atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct
    18781 aatcgaggac caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa
    18841 agaccgatgg ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg
    18901 ttttgtccaa aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc
    18961 acatttgctg gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt
    19021 tcatctttcg ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca
    19081 aaagctccta aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct
    19141 gtcatcgcca tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa
    19201 atatagacag ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa
    19261 gcctgtgctt tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact
    19321 aggatcagtg tcccaataaa ctcccccatg atttgagtca tatcactgtt catttatttc
    19381 tcccccttta gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt
    19441 tgcgccttct cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc
    19501 ttgatctcat caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa
    19561 tcgcctgggg ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg
    19621 agactcaaac caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta
    19681 gtcccgtaaa aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct
    19741 tcttttgcta attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa
    19801 atctgatatt ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat
    19861 tggcgtatca atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca
    19921 gataaagtca gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa
    19981 ggtgcttttt catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga
    20041 gaaactttgt tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct
    20101 gaaatcgatc cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga
    20161 cgaagtcctg cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga
    20221 tgattgatca catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa
    20281 tctccttggt aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca
    20341 aaaaccatcc gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt
    20401 tttttcgcat ctacaactaa atggatccct ttagttggac gcattttagg agagactgga
    20461 cgcgtaaaat tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat
    20521 tttgctttga tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt
    20581 tgatcgccat catataaaaa gccaaccgct ttcattttac ttactagata agccccatct
    20641 tcggcagcct ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga
    20701 tagacacccg cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct
    20761 tctggtgtca gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa
    20821 aggtccattg ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag
    20881 attggcaaaa gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca
    20941 cgttcaccaa ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg
    21001 ccatgaacaa gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc
    21061 aatcctgttt tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg
    21121 ccaccgatga tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt
    21181 gtcttgtttg aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc
    21241 ttttttccaa ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag
    21301 cttgccagct tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa
    21361 tcctgctaaa aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg
    21421 tacattcaag atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc
    21481 tactttcaat acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct
    21541 cacttgataa gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg
    21601 tgtcaagcca aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac
    21661 gaatgcagga acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga
    21721 atcgctagct ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc
    21781 cacaaaaata cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt
    21841 cgtcaataaa ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga
    21901 acctgtacca taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc
    21961 ggcttgctgg tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc
    22021 ttgcgtataa ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat
    22081 atttagtaaa tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt
    22141 ccgactggca ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa
    22201 ccaagaatcg atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc
    22261 tacatgatca agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc
    22321 tgttttttcg tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc
    22381 tgactgtctg gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca
    22441 tattactgtt gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat
    22501 ccctgattcg atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc
    22561 atcatgttcc acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc
    22621 gatgtgtctt cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat
    22681 cgccatgata tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct
    22741 ttaactactc ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt
    22801 tttctttttt tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt
    22861 cagcgtctct attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag
    22921 ataatattca cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt
    22981 accttcttca taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata
    23041 ttgttgaaaa gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg
    23101 ttttgcaata taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact
    23161 aagctcacta tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt
    23221 cgccagtaac tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc
    23281 acgctcatag acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat
    23341 ttggtagcct actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa
    23401 catcgtttcc cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa
    23461 atcatactga taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa
    23521 catcattgtt tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat
    23581 tggatcgatt gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt
    23641 tgcgtacgta cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat
    23701 ctgtgcttct gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa
    23761 cggtcttttt tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg
    23821 gaatagttca taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt
    23881 gatttgtaat ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga
    23941 agactcactg atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa
    24001 taaatagacg agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc
    24061 caaacttaca tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac
    24121 ttttttttcc atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc
    24181 ttttgacagt tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac
    24241 aaggtttcgc aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt
    24301 cataggcagt attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga
    24361 aaaatagctc atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca
    24421 gttggtcaag aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt
    24481 tttcagaatc aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt
    24541 agtctacgta tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac
    24601 ctacgcttcc taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat
    24661 cctttgtctg tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat
    24721 ttactagaga aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc
    24781 caatcgcata aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat
    24841 catcattcaa tgttaattgt tcttcccaca tctgattgtc gggacgagaa tgattagcca
    24901 gcaaaacaag atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt
    24961 cggtcaaaga cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct
    25021 ttactaacgg ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt
    25081 cgtaaagatg gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt
    25141 gtcctgcacc aataattgca attttctttg cattcatgat cttttgctga agagccgtta
    25201 cgttttgtat ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact
    25261 tttgatttga accagtcgct agaatcaaac gatcaaaatc caattttttt cctcctgcta
    25321 aaacgacttt tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat
    25381 agcggtcagt cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc
    25441 cttttaatat caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca
    25501 aatatatttc acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa
    25561 acgacccgcc gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact
    25621 gctaacttct caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata
    25681 gcgatgcagc agaatcaacc ctctccatca taccgtgttc taggtctgct tctcaataat
    25741 gtactagtgg acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc
    25801 ttataaaaga aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa
    25861 cgaaaagacg agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt
    25921 taattcttta atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc
    25981 cctgtaagaa gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag
    26041 tttgctcctt tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca
    26101 tttgcttcct cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt
    26161 ccagactcat ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc
    26221 ccttcatttc gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc
    26281 cacattgttc cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca
    26341 tcaggacgta aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc
    26401 caatcgattt catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta
    26461 ggactgacaa aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt
    26521 tcacttaacc acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat
    26581 acgatcaata ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg
    26641 ctttcaagaa attatataat gggcttggca aataaagctc tttatatgct ttttcaaaag
    26701 gtttcgttac aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga
    26761 cgatcatttg ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt
    26821 cgcgattgcg gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga
    26881 ccacttcaag aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat
    26941 ttaatgaaac atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg
    27001 tgtcttcaaa acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga
    27061 tgaatggttt tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa
    27121 taaaacgata acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg
    27181 gagagaaaaa ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc
    27241 ctgattcaat cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt
    27301 cttcactcat tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag
    27361 atacaggctg ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct
    27421 gaataatcgc ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa
    27481 tcatttcatc atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat
    27541 ttgcagcacc agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct
    27601 catcttcggt gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca
    27661 atcggttctt caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg
    27721 tcattcctcc cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact
    27781 cgattagttg tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct
    27841 gttgcatcat tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg
    27901 tatgactgat gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt
    27961 tctgtaagat aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat
    28021 tctaattttt caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata
    28081 tctttttgtg gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt
    28141 gccagacctg tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt
    28201 ttcacgctaa agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc
    28261 cctccattgg cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa
    28321 tgtccgcctc ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc
    28381 ttgttttcga caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt
    28441 agttttgagt atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
    28501 gcttaatacc gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg
    28561 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt
    28621 tgagctaatg ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc
    28681 gttgaactcc gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat
    28741 ggtgacttgt gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt
    28801 gacaagactt ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg
    28861 gatgacttgt ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc
    28921 ttcgtcatct tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc
    28981 gctgacgata tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat
    29041 agcttccttc atctgctcaa tatgctgctt caaatagtca atgcggtaat cgtcttgaac
    29101 ggttccatct tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg
    29161 tttttgatac ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg
    29221 ccagccccat tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc
    29281 ttggacccct tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa
    29341 agaaacaaaa tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa
    29401 gttgacacct ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg
    29461 aacatcacta aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg
    29521 atcacaagaa tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt
    29581 gataatttca tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc
    29641 ttggtatttc gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa
    29701 agggtgatgt tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt
    29761 atagcgtttg aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg
    29821 ccatccacca tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac
    29881 tggctcaatc ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc
    29941 ttcattgggt gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg
    30001 atagcattta aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa
    30061 atcatttccg tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac
    30121 ttgctgatgc gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc
    30181 gaatgtatac acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca
    30241 ttgattggcc gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat
    30301 ccccctaaac ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt
    30361 ttgaatctac tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt
    30421 aattcttgca aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat
    30481 actgcacttt gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt
    30541 atctctgctc cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa
    30601 acttccccgt ttacagaact gtagattttt ccgtccgttg gaatgatcgc aaacccttct
    30661 cctaaagatc cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct
    30721 gctgcaggag tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag
    30781 ccgaaaagaa aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc
    30841 caaacaaatc cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc
    30901 aaagcaccgg ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca
    30961 atgtaaaatg gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca
    31021 aataatgctg tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc
    31081 aaaaataccc caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct
    31141 agcgtatcac gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt
    31201 aacccaaaaa tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg
    31261 ctaaagttat aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt
    31321 ggacctacaa ctaataaaat aattgggacc atcacgatga tacaaatgaa tggtgtcaat
    31381 aaatttcgaa tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc
    31441 catacagcta aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc
    31501 cctagaaatc gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga
    31561 gctgctgcaa taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat
    31621 gccaaaaata taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa
    31681 gttcctgatt cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc
    31741 aaaccacttc ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata
    31801 aaagagttta aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct
    31861 agtttcattt gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact
    31921 tgaaacattc ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc
    31981 ttatctgcct tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca
    32041 ttgctgacat tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag
    32101 tccattttta cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta
    32161 tagttcttag aaaacggttt ctccattcaa ttagtacccc ttctaatagc gaacgaaact
    32221 gtacaatagt gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca
    32281 agtatatcgt ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc
    32341 tgatgtataa gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg
    32401 ataagacagc acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga
    32461 gagattagtt gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg
    32521 tagtaatgat caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc
    32581 aaaatctgat aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa
    32641 tcatcccact caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt
    32701 ttgtttgatt gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg
    32761 ttcatacttt gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta
    32821 acaatcagta tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca
    32881 aaataagcgc tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga
    32941 attaaataaa tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta
    33001 ttatgtccgg catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt
    33061 tcatggaaca ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct
    33121 cttagattct tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct
    33181 aatagtcgat tttca
//
LOCUS       DAFGFZ010000017        33094 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000017 DAFGFZ010000000
VERSION     DAFGFZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.017"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            184..1095
                     /locus_tag="LR726_000668"
     CDS             184..1095
                     /locus_tag="LR726_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL6215777.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1473..2390)
                     /locus_tag="LR726_000669"
     CDS             complement(1473..2390)
                     /locus_tag="LR726_000669"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL6215778.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2592..3029
                     /gene="zapA"
                     /locus_tag="LR726_000670"
     CDS             2592..3029
                     /gene="zapA"
                     /locus_tag="LR726_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL6215779.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3087..3635
                     /locus_tag="LR726_000671"
     CDS             3087..3635
                     /locus_tag="LR726_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL6215780.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3750..6110
                     /locus_tag="LR726_000672"
     CDS             3750..6110
                     /locus_tag="LR726_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL6215781.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6197..6511
                     /gene="trxA"
                     /locus_tag="LR726_000673"
     CDS             6197..6511
                     /gene="trxA"
                     /locus_tag="LR726_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL6215782.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6693..8471
                     /gene="uvrC"
                     /locus_tag="LR726_000674"
     CDS             6693..8471
                     /gene="uvrC"
                     /locus_tag="LR726_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL6215783.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8616..9515
                     /locus_tag="LR726_000675"
     CDS             8616..9515
                     /locus_tag="LR726_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6215784.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9508..10770
                     /locus_tag="LR726_000676"
     CDS             9508..10770
                     /locus_tag="LR726_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215785.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10931..12829
                     /locus_tag="LR726_000677"
     CDS             10931..12829
                     /locus_tag="LR726_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL6215786.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(13159..13878)
                     /locus_tag="LR726_000678"
     CDS             complement(13159..13878)
                     /locus_tag="LR726_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL6215787.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            14215..14547
                     /locus_tag="LR726_000679"
     CDS             14215..14547
                     /locus_tag="LR726_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215788.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14534..16537
                     /locus_tag="LR726_000680"
     CDS             14534..16537
                     /locus_tag="LR726_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL6215789.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16556..17026
                     /locus_tag="LR726_000681"
     CDS             16556..17026
                     /locus_tag="LR726_000681"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL6215790.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17047..17631
                     /locus_tag="LR726_000682"
     CDS             17047..17631
                     /locus_tag="LR726_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL6215791.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17646..18632
                     /locus_tag="LR726_000683"
     CDS             17646..18632
                     /locus_tag="LR726_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL6215792.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18622..18933
                     /locus_tag="LR726_000684"
     CDS             18622..18933
                     /locus_tag="LR726_000684"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL6215793.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18955..20736
                     /locus_tag="LR726_000685"
     CDS             18955..20736
                     /locus_tag="LR726_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL6215794.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20729..22105
                     /locus_tag="LR726_000686"
     CDS             20729..22105
                     /locus_tag="LR726_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL6215795.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22112..22747
                     /locus_tag="LR726_000687"
     CDS             22112..22747
                     /locus_tag="LR726_000687"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL6215796.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22777..24132
                     /locus_tag="LR726_000688"
     CDS             22777..24132
                     /locus_tag="LR726_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL6215797.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24324..25718)
                     /locus_tag="LR726_000689"
     CDS             complement(24324..25718)
                     /locus_tag="LR726_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL6215798.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25810..26781
                     /locus_tag="LR726_000690"
     CDS             25810..26781
                     /locus_tag="LR726_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL6215799.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26778..27560
                     /locus_tag="LR726_000691"
     CDS             26778..27560
                     /locus_tag="LR726_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL6215800.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27729..28100)
                     /locus_tag="LR726_000692"
     CDS             complement(27729..28100)
                     /locus_tag="LR726_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL6215801.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28234..28677
                     /locus_tag="LR726_000693"
     CDS             28234..28677
                     /locus_tag="LR726_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL6215802.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28720..29529)
                     /gene="yidA"
                     /locus_tag="LR726_000694"
     CDS             complement(28720..29529)
                     /gene="yidA"
                     /locus_tag="LR726_000694"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL6215803.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29548..30915)
                     /locus_tag="LR726_000695"
     CDS             complement(29548..30915)
                     /locus_tag="LR726_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL6215804.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30899..31729)
                     /locus_tag="LR726_000696"
     CDS             complement(30899..31729)
                     /locus_tag="LR726_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL6215805.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31892..32329
                     /locus_tag="LR726_000697"
     CDS             31892..32329
                     /locus_tag="LR726_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL6215806.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32405..33025
                     /locus_tag="LR726_000698"
     CDS             32405..33025
                     /locus_tag="LR726_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL6215807.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        10771 a         5666 c         7115 g         9542 t
ORIGIN      
        1 attgggtaga tgaatcacca cgaatagtca aaaggagatt tcaggatatg ccaaaaatga
       61 aaaaaatcat tttagcagta atgttatttt ctacggttat atcttttaca gccccttttt
      121 tgcttactta agctgatttt aagttttggc ccttagtctg gatctacgag gtgagagtca
      181 agaatgaaaa aaataattgg agcttttgta tttgcaatca ttatcattat tatctgtttt
      241 caaatagatg tttttggtca aaaaaagata gttggacaaa cggtcagcag tacgaacagt
      301 acggttacaa agacaaagga atcatttgat gatacagaac aagagacatt aatcaaaatc
      361 ggaaaaaatg accgtacact atatacagat gactcgctaa ctaccccact tgcaactata
      421 aacggcggag agctggcgga gtttctttct gaaacaaaaa atgcttatca gatcaagaca
      481 aatgatggct atactggata tttagccctc gaagatggaa ccaaagtcac aaagaatata
      541 caaacaaaac cggaagagct atctgatgca gtgattgtat tagatcctgg gcatggcgga
      601 aatgatactg gggcattaag caatgatgaa caaacagaag aaaaagatat aacattaagt
      661 acagccataa aagtaaaaaa ggcgctagag gatgctggag caacggttta tttgacccat
      721 actacagatg aattagtaca gcttggagat atatgcgact acagtgaaga aaaaaaggca
      781 gatgtcttta tcagtttaca cgcagactct acggaatatg cgaatgaagc aactggaatc
      841 acgacatatt attactatgg acaagaggaa acattagcac agacaattgc tgactccttc
      901 actgatctgc ctctggattc aagaggaatc tccactggga actatcaagt tcttcgagaa
      961 aatcttcaac cttctatttt aatcgagatg ggttatatga ataatgatag tgatttaaag
     1021 gaattagtaa cagacagtta tcagcagcag attgctgcaa gtttgacaca agcattgacc
     1081 acttattttc agtaataaat aagcccagcc aaacaaaaat agctcctcat atttttcgtt
     1141 tggccgggct tattatcgca gatgcaagct tttaaacacc gtttaaggaa gaggtcgtga
     1201 gcctgccgtt caactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttga
     1261 tgactattgt gacttgtgac cgaggagttg gcatgagaac accgtttaag aaaaaggttg
     1321 tgaaatcaac gttttgttct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
     1381 ttgccgtttg ttgagctaat accgaggatc agatgattga acaccgttta ttcggaacaa
     1441 gggactgtga catgactttt gtcacagttc cattattttt ttagtagctt caaagctttt
     1501 tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc gcctttctcc
     1561 aatagttttg ccgctacttg gtcagatttc gttccagctc ctgacggaag ggtgattcca
     1621 gcttcagctg atgctttgtc taactcttcc aaaaagctgg cacgactgat gatagaagca
     1681 gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt ttcttgtaat
     1741 tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa ctgatcgatc
     1801 aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat cgcttgattg
     1861 tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt gtatttttct
     1921 ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc aagctggatg
     1981 atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa ttctgcaagc
     2041 tgatcacgtt tcacataagc agcacatact gttagcggac cgaaatagct gccatttccg
     2101 acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga agaggctgtt
     2161 tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc tattgctgca
     2221 ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt cccgtctttt
     2281 tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg atagtatgct
     2341 ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat aaaattctcc
     2401 tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta agagattgcg
     2461 agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa aatatttgtg
     2521 gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc aaataggagg
     2581 aaatgattcg tatggcgcac gaaaaaacac gctacaaagc ggtgattgcc aaccaaacat
     2641 atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag ctgatcaatg
     2701 aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa gcagcaatct
     2761 tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaata ttggagctgg
     2821 aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt gaaaatcgtg
     2881 tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa aatggtcaag
     2941 cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag aaacgaaaag
     3001 gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga aaaatttaaa
     3061 aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt ttattgctaa
     3121 ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc gcgattggat
     3181 atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt catttggaat
     3241 tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt gatcaggtat
     3301 ttgcctttca cctagatgaa gctttttatg caggaactgc ttttctgatt attttgctaa
     3361 ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg tatgtgccga
     3421 tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg gcttacgtta
     3481 ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc caaaatcaat
     3541 tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt accaacaaga
     3601 tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg aaaatatttc
     3661 gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg gcacagctct
     3721 tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa acattggaat
     3781 ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga aaagaagaac
     3841 tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt caagaaactg
     3901 aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa attgaaaata
     3961 tccgtcccca tatgaaacgg attgagatcg gagcagactt gaatggagtg gagcttgcac
     4021 aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat gatctttctg
     4081 acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc acgatccctg
     4141 ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact gatgacgctt
     4201 ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct gttcgtgaac
     4261 agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg atcatcacca
     4321 tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta tttggcggtg
     4381 tagtccacga tcaaagtgct tcaggtcaga ctttatttat tgaaccaaaa caagtggtgg
     4441 atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt caacgtattt
     4501 taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac aatacttatg
     4561 tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa gtaaaagcca
     4621 tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga catccactga
     4681 tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat caagccatcg
     4741 tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta ggtctgttac
     4801 aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa atcggtattt
     4861 ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt ctatcgacct
     4921 tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa aaaagtctgg
     4981 tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct ctagcaatcg
     5041 caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg cattatcctg
     5101 aattgaaagt ttatggatat aatcgggcaa atacgattaa tgccagcatg gagtttgatg
     5161 tggatacatt aagtccaacg tatcgtttgc tgattggcgt tcctggacga agcaatgcgt
     5221 ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg aagcagttga
     5281 tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat cgccggaaaa
     5341 tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa gaacttcacg
     5401 atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa atggaaaaag
     5461 caaagaaaaa agcaaatgaa gtcgtgtcag aggcggaaga aaaagcagaa aaaatcattg
     5521 ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag catcagttga
     5581 tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag aaaaataaag
     5641 tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg ttggttacga
     5701 cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa gtagaaatcg
     5761 gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct caaaaagagc
     5821 cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg ccgaatcaat
     5881 tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa tatttggatt
     5941 ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga acaggcgcac
     6001 ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc tttgagtttg
     6061 caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa agcttactaa
     6121 aggtaagcag atggttcgct atccaagtat agaaatgcta tactagtcat agattttaaa
     6181 aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg aagaaacaga
     6241 taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc gtatgcaagc
     6301 accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa ttgctaaaat
     6361 ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca ttccaacatt
     6421 aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt caaaagaaca
     6481 actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa gctgagggtg
     6541 ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta ttcaaattaa
     6601 tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga catgctataa
     6661 taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa aacaaattag
     6721 ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga acgatcattt
     6781 atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact ggtagccata
     6841 atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc gttacagaat
     6901 cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat cctaaataca
     6961 acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat gaaaaatatc
     7021 cccgtttggt gattactcgt aaggtactaa aagacaaagc actgtatttt ggaccatatc
     7081 ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt cctctgagaa
     7141 aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga caatgcctgt
     7201 gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat gaagtgaaaa
     7261 actttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa atggaaaaag
     7321 ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc cgtgccattg
     7381 agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga gatgtatttg
     7441 gttatgcgat tgataaagga tggatgtgtg tccaagtgtt tttcgtaaga caaggaaagc
     7501 taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa gatttcttga
     7561 cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa gtcttgattc
     7621 ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg ctgcaaccaa
     7681 aacgaggaga aaagaaaaaa ttagttgaat tggcgagtaa gaatgcctca gttgctttga
     7741 atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca gttgaacgtt
     7801 taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat aactctaata
     7861 tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa ccggcaagaa
     7921 aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat gcatcaatga
     7981 gagaagtcat ctatcgcaga tattcgcgag tgattcgcga agggttgcct ttacctgatt
     8041 tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc ttagaaaatc
     8101 aattaggaat cgatattcct attgctggca tggtaaaaaa cgataaacat aaaacaaatg
     8161 agttgctatt cggtacagat ttggctgtag taccgcttga gcgaaattca ccagagtttt
     8221 tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc catcggcagc
     8281 tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta ggaccaaaac
     8341 gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca gccacgacag
     8401 aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt gataaattac
     8461 atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca ttttatgtac
     8521 aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc tggtgtctaa
     8581 ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag gatcttgtta
     8641 aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc gatggaaaaa
     8701 tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt ttgatcttgg
     8761 attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt ggggagaaag
     8821 attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa gtaacgattc
     8881 aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag attgaaccaa
     8941 aaatcgactt ttggatggaa aaatttcagg taaaagggaa aaagacggat aaagtaaaat
     9001 ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc catgaaccta
     9061 aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct gaattgctaa
     9121 aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct agccacaata
     9181 tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga aaaatggttt
     9241 tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta ttcttggaat
     9301 ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg atccttcatg
     9361 aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa atctttactc
     9421 gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact ttggaagaga
     9481 ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac caaagatgtc
     9541 tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc ttttatcatg
     9601 ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt caataaaatc
     9661 ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa aacagatgat
     9721 tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc ggaagaaaaa
     9781 atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat gctttatagc
     9841 gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac tggcttgcaa
     9901 tcgatgcttc gagcgagtca attaggcatt tcaccagaag aagtagctag cttgagccaa
     9961 caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat gacagtcgga
    10021 gaagataact cgacagtcca atacgtcgta agttatgtag caacgattgt cttgttcatt
    10081 attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa aggcacgcgg
    10141 atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg aaaattaaca
    10201 ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt tggtatagga
    10261 tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat ctcattggat
    10321 aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg cattttgatt
    10381 ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga tacagcaaaa
    10441 gcaattttgc cagtgactta tctatctcta ggcggataca tgcttggatt gatactagga
    10501 gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt cttatcttca
    10561 tatattatgc cgattcgctt agcaaatgag acagtcggta tgggtggagc attgatttcc
    10621 ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa catgtacaaa
    10681 tcgaatgtac tcgtttatag cgaaggcgga ttatggagtt cattgaaaca atcgatttca
    10741 attatgagga atgaacgaaa aaaagcataa aacaatccta aaatacagaa ggaagctgga
    10801 catctattgt tcccagcttc tttttgtata gacctatttt atttatttta ctatattgga
    10861 tatatattca ttgaaaatag tgtataacaa ctttataata ttgagtatac caatttttgg
    10921 aggcgtttta atggaccgtt atttagattt actttcagaa aaattcccct caacggagga
    10981 tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag gaacagagtt
    11041 atttttgagt gatatacatg gcgaatttcc tgcttttgat catattctgc gtatcggttc
    11101 aggaaatctt aaagaaaaga tcaaagacct tttttctgaa caaatgacgg aaacggatat
    11161 cagccaattc actattttta cggcttatcc agaatatgca ttaacgactg attggtacaa
    11221 agaacaagat aaatcgcagc tgatccatca tttgatcgat cttttacggt ttacgacagt
    11281 caaatatacc cggtcaaaag ttcgaaaagg attgccgaaa gaatatagct atatcattga
    11341 ggaactcttg tatatcgatg accgtatcaa tggaaaaaaa gactatgtaa aaaaaatcat
    11401 tgaacaactt gttttgatga aggaagaaga aaaattttta acgaaacttg cacagacgat
    11461 ccaaaaatct gtcatcgatc atttgcatat cgttggagat atttttgacc gaggaactca
    11521 agcagccaaa gtgatggatc gaatcatgga tttttcttct gtagatatcc aatggggaaa
    11581 tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttac tgacactttt
    11641 acggattgca gcacgctata attatttatt tgaattagag aaagaatatg ggttgaattt
    11701 acgtccattg ttttcttttg ctcatcaaac ttatcagaag aatccagtat ttgcgcctaa
    11761 aaacagtaaa aatctatccg aaagagagca agaagaatta gaaaaaatcc atcaggcact
    11821 ttctatcctt caattcaaat cagaacatca gttattggat cgtcgacctg aatttaaaat
    11881 ggacgatcgt aaattgctgg aaaaaatcga ctatgaagca tcaacgattg atttagaagg
    11941 tcagctctat gagttgaaag gaacttgttt tcaaacgatc cagccagatg atccaaaaaa
    12001 attcttggaa gaggaacaaa aagtgattga tagtttgatg tattcattcc agcactcttt
    12061 gagaatgcaa aaacatatga cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa
    12121 tcagcatgtg ctgtttcatg gctgtatacc tgtagacgag aagggacagc tcttagcatt
    12181 tgaattagac caatcttatc gcggaaaaga actgctgggc ttttttgaaa aacagataac
    12241 tgaaagcgca aaagatctga ctgcaaaaga agatttagca accgatttga tttggtatgc
    12301 gtggagtggg ccattttcgc ctttattcgg caagagtaaa atggcgactt ttgaacgtta
    12361 ttttctcaca gagcctcata cgcacgtcga gaaaagcaat ccttattatc acttgcgaga
    12421 tgaagagtgg ttcagtgaaa aaattttagc agaattcgac acaaaagaag aagggtcagc
    12481 aatcattaat gggcacacgc cagtaaaagt aaaaaaaggg gagtcaccga tcaaagcaaa
    12541 tggaaaagta tttgtcattg acggaggttt atccaaagct tatcaaaaaa cgacaggaat
    12601 tgccggttat tcactattat gcaattctta tggtttccaa atcgtgacac attatccgtt
    12661 tttgcctatt gaacaacaat ttgcaaaaaa atccgaccaa acaaatgtga aaaaagtaat
    12721 cgaacagcaa ctaccacgca aactcaatcg tgatacaaca aaaggtatgg aactgcagca
    12781 gcagattacc cagctcaaaa ggctgttgga ctatcgaaag gacgattgag aagaattcca
    12841 gtgaacattg ttccatagac tggagactga gacaaaagtc gtgtcgcagt ctctttttcc
    12901 ggataaacgg tgttcttctg ccaactcctc ggtcacaagt cacactattc aacaaacatg
    12961 aaaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct
    13021 cttggtataa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
    13081 tgtgaaaagc cattttcaca aaatttttct taatactcga aactaaacag ctttttcaca
    13141 accccctcag aaagtctatt aaaattcatt cacaaagaaa tcgaataagc gctgttcaga
    13201 tggatgtaca cggtgggtaa gttccggatg ccattgaaca ccgaggattt tatgcatatc
    13261 atctttgctt tgaaccgctt caacgagtcc atcgttagaa aaagcgatgg cttttaataa
    13321 aggtgaaaga tctttgactg cctgatgatg gtaagaattc acaaaatatg gatcggggag
    13381 aaggttgctt aacaaactgt ctgaaacaat cttgacttcg tgagtcgtaa attgcggggc
    13441 agtgggctgt tgttcatgtt ttactgacca ctttggatat aatgaaagat cttgataaag
    13501 tgtaccttcc aaaacgacat tcaatagttg gagtccccgg cagactgcaa aaattggttt
    13561 tctttgtttc aatgcttcca agatcagtgc ctgttcaaat tggtctcgtt gaatatttgt
    13621 ttccgttagc tttggatgag gatcttccat gtaaagttgt ggactgacat cttgaccacc
    13681 tgcaagaata agtttgtcga tttgtccaat cagttgagga gctgaatctg gagacatgac
    13741 tggcagaata agaggcaatc cttgagcatc taaaactgca tccacgaatc cttgcggtgt
    13801 atagctcact tgatttcctt ggaaagtatc tgttgctcga atcagttgat ttccggcaat
    13861 gccgatgatt ggtttcattt gtattccccc taaagtagac ttattgtaac aaggatttaa
    13921 aaaaaattaa actatgtaag acttgcccct atacttatct gttcttgctg tgttactatc
    13981 tgtaaagaga aatttcgtgt gaaattcatg acgatagttg aaaatattgc ttaatttgtt
    14041 ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc
    14101 aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat ctttagaggt
    14161 acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag
    14221 caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga aaagcgtgct
    14281 caaacactgc ggcagcagct gaagatctat gcaacagaaa aagagcagga attggaaacg
    14341 atccaaacaa actatcgaca acaactaaaa cagttggtgg aagaaaaaga acaatttgaa
    14401 atacaagaat tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca
    14461 actttgaaag atcaatatca aaagcatcag agtcaagcga tcggtgcgat cattgaaagg
    14521 gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg accctcattt cagataaaag
    14581 aaatcaggag aggattttac aagcagtaca aggaatccaa aacgttgaaa tccgggatct
    14641 gtatcaaaat agaacaaaca accagtgggt ggagaagtat tttccagata ccacgattat
    14701 tgataaagaa gcaaaattaa gcgctttagg taatcggttg aaagacatcc aagaagctat
    14761 ccaattcata caacaccacg gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga
    14821 actttcctta caagatttgg aagccaatta ttccgaagaa aatttcataa ataaattgaa
    14881 agaagtcctt gatttaaaag aaaagtggga acaactaact gaagaacgtg atcgtttaga
    14941 agaagaggaa gaatggcttc ttcattggca acagctggat attgctccaa gtatgtatgc
    15001 aagtaagacg accatttttg agatggggac tgtgagtgca gcaaattttg aaagtttcaa
    15061 ggaagaactg agtaagcttg atgacgttta tatggaagaa atagattata cgccaaaaca
    15121 agtttatctt tcgtatgttg ctttaaaaaa atcagttgct gttatagaag aaattgctgg
    15181 gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag aaaaactgca
    15241 agagaccaag cagcaattat ccattgttca cgagcagcaa aagaagataa cgacagcgtt
    15301 aggtcagtgc agtggctata tccaagaatt tgaatgggta gaagaagtca cacttgccgt
    15361 tgcagaacga gaaaaaataa aagaacggtt tatccatgcg acttatctga tcgttttgca
    15421 aggttgggta gatgcagaag aaaaacagaa tttgctgcat gtgttaaacg aaaccgtatc
    15481 agaagaagat atatacgtca gtttcgaaac accaaactca gaagagattc agcaggaagt
    15541 ccctactaaa ttgaaaaacc atccgcttgt cgaacctttt gaattgctga ctgaaatgta
    15601 cagtctgcca aaatatgaag aagtagatcc aacaccttgg atgacaccat tttatcttgt
    15661 cttcttcggg atgatggtcg cagatgttgg ttatggtttg ctgatgctca ttgggacaat
    15721 actagctcaa aaactacttg ttctaccacg cgggatgaaa cgtttcgtaa aattctttga
    15781 gattctctcg ataccatcga tcatctgggg attgatctac agttcatttt tcggacagtc
    15841 attaccaaaa gaactgtttg gcatccgctt gcctttccca atcctatcta cgacggatga
    15901 cgtcaatacg attttgattt tatccgtgat tttcggtctg atacagattt tggttggatt
    15961 atttgtggca gcaaaggaaa atatcaggag aaaagattac ttgagtgctg tcagtgatgg
    16021 atttgcctgg caagggatcc taattgggat cgttattgcg ttggttggtg ccatgttgat
    16081 aaaaaataca gcatttgttt accttggcgc tgggctatcg atcattgctg ccttatgtat
    16141 cttgatcgtt ccgattatcc aaacgccgtc taaagtcaaa ggtgctgcca aaggtgcgta
    16201 taatctttac ggactgaccg gctatatcgg tgatttagtc agttatacac gtttgatggc
    16261 attgggtatt tcaggcggaa gtattggagc ggcattcaat atgctagttg ctttcatgcc
    16321 gccagctgct cgattcagtg tagggatcct tttgatcatc gctttacacg cactgaatat
    16381 gtttttgaca ctgctaagtg cctatgtcca tggcgcacga ttgcagtatg tcgaattttt
    16441 tgggaagttt tatacaggag gcggacgtgc ctttgatccg ctgaaaacgg cggaaaaata
    16501 cgtcaacatc aatcacaaga aaaaaaacaa taaataattg gaggattttt atataatgat
    16561 ggattactta attagtcaaa atggtggaat ggtatttgcg gtattagcga tggcaacagc
    16621 gacgattttt tcagggatcg gttcagctaa aggtgttgga atgacaggtg aggcagcagc
    16681 agcattgaca acaagtcaac ccgaaaaatt cgggcaggct ctgattctgc aattgcttcc
    16741 aggcacgcaa ggtttgtatg gattcgttat tgcctttttg atttttatca acttaggaaa
    16801 tgacatgtca gttgtacaag gactgaactt tctaggtgct tctctgccga ttgcttttac
    16861 aggcttgttt tcaggtattg cccaaggaaa agtcgcagcg gcaggtattc aaattttagc
    16921 gaaaaaacct gaacatgcta caaaaggaat catttttgct gcgatggttg aaacatacgc
    16981 gattttaggt ttcgttattt ccttcctatt agtgttgaat gcctaagcat aaaggaggaa
    17041 aagcagatga acgccattga aaaaatcatc tctcagatga acgaagcagc agagcaggaa
    17101 agagcggcgt tagaacaaga agagcgaatg aagatcgatc aaaactttga acaaaaacgt
    17161 acgcaagttg aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt actcgaaaaa
    17221 aaataccgtc agctaaggaa tcgccagcaa gtagaagtac gacaagaaaa tttgaatgcg
    17281 aagcaggaat ttttaagacg attgtttgca gatgcggtaa ctgaaatgga aaattgggat
    17341 gaatctgaac aaatccagtt tatcaaaaat gcattgtatt cattaccttt gacaggaaaa
    17401 gtagcattca ttgctggaga aaaatcagca gcctatcttt ctcagacgtt gttggatgaa
    17461 tggaacaatg aacttccctt tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg
    17521 ggttttctga taaacgatca aggagttcag tataattttc ttttcagcag ccttgtacaa
    17581 gatatccaag gaacgatgag ttttgagatt gccaatcaat tattcgaata aggagggatc
    17641 agtgtatgga ataccacgaa ctaaatccct tgattcgcgg aagagagctt gaacttttgt
    17701 ccaatgagac atttgaacgg atgattcaaa caaattctgt cgaagcactt ggtgaaatat
    17761 tgaaatccac gatttacagt ccatatattt atgacggata tgaatatgac ttcgaacaga
    17821 atcttttaaa ggagcagagc cgtctatttc gctggttaaa ggaatcagca ccggagcctg
    17881 aaatcgtttg gatctatacg atgcgttata ctttccataa tctgaaggtt ttgacaaaag
    17941 ctgagatgac aaatcaaaat ctggaccatt tgtatatcga cgatggattt tattcattag
    18001 aaaccttgaa ggatgccatt catacgcaag tttcaaatga acttcctgaa agcattatgg
    18061 acagtatacg agaagtacgt gattattttg aagagtctac cattcttcag ggaattgacg
    18121 tgatttatga tcgacagttt ttgacagagc agcgacggct tgccgataag ctaggatatc
    18181 aagaattgct gacagaagtc attgctttta ttgacttgac caatatcaca acgatggctc
    18241 gaggaatatt gcagaaaaga acatatgggt tcatgacgac ggtactttcc agctcgggaa
    18301 gtatcgaaaa agaagtattt ttgaactttg tagataaaga tatggaagat tacacgaatt
    18361 ttctattaga gacagattat gctgattttc tgcgccctgt catccgtgag ggaaatattg
    18421 atctcgtaaa gcttgaacag ttgaaagatg attatctaag ttcattgtat caagaagcac
    18481 aaacccaggc tttcggacca ctgccactac tagccttttt gaacgcaaaa gaggtagaaa
    18541 gcaaaaacct tcgtttactg gttatcggca aaagaagcca tttcgaatca gaaacaatca
    18601 gaaagcgggt gagacaagtc tatgacgcat aagatcggtg tagttggcga caaagattct
    18661 gttttgccat tcaaattgtt tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt
    18721 cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt cacagagcaa
    18781 tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg aacaaatgat acctgctatc
    18841 gtattgattc ccaatcatca aggatcatta gggatagggc tggaagaaat tcaaaaaaac
    18901 gtagaaaaag cagtaggtca aaatatctta tagtatgaaa aggagataga aattttgcaa
    18961 attggaaaaa tcataaaagt ctccggacct cttgtaatgg ctgagaacat gtctgatgcc
    19021 agtatccaag acatgtgtct agtgggagat ctaggagtta tcggagaaat tatcgagatg
    19081 cgtggcgatg tggcgtctat tcaagtatat gaagagactt ctggaattgg tccaggcgaa
    19141 ccggttcgct ctactggtga agctttatct gttgaattag gaccagggat catttcacaa
    19201 atgtttgacg gaatccaacg gccattagat acctttatgg aaatcacaca aagtaatttc
    19261 ttagggcgcg gagttcaatt gcccgcattg gaccatgaga aaaaatggtg gttcgaacca
    19321 acagtagaag ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca
    19381 aaagttataa aacataaaat catggtgcca aatgggataa aaggaacgat caaaacaatc
    19441 gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac
    19501 aaaaatttga cgatgcttca aaaatggcca gtaagacgta gtcggccaat caaagaaaaa
    19561 ttaaacccgg atgcacccat gatcactgga cagcgagtga ttgatacatt cttcccagtg
    19621 acaaaaggag gagcagcagc tgtacctggt ccttttggtg cagggaaaac agtcgttcag
    19681 catcaaattg caaaatgggc cgatgtggac atggttgtct atgtgggatg tggcgagcgt
    19741 ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat tgattgaccc taatacagga
    19801 gaatcgttga tggaacgtac ggtattgatt gctaatacat caaacatgcc agtagcagct
    19861 cgagaagctt ctatctatac gggaatcaca attgcggaat atttccgtga catgggttat
    19921 gacgttgcta tcatggcaga ctccacttca cgatgggcag aagcacttcg tgagatgagc
    19981 ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag cttatcttgg ttcacggctt
    20041 gcggaatact atgaacgatc tggaagagtc attgcattag gatcagaagg tcgcgaagga
    20101 agtataaccg caatcagtgc tgtatccccg tctggagggg atatttcgga accagtcaca
    20161 caaaatacat tgcgggtcgt aaaagtattc tggggtcttg attccagttt agcccaaaaa
    20221 agacattttc cttcgatcga ttggatacaa agctattctc tttattcaac agaagtaggg
    20281 aagtatatgg atcatatcct tcaagctgac tgggcatcca tggtcaccga gggcatgcgt
    20341 attttgcagg aagaacagca gctgaatgaa atcgtgcgtt tggtagggat tgattccctt
    20401 tcagataatg accgtctgac attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag
    20461 caaaatgctt ttgatgatgt cgatacattc acttcaagag agaaacaatt caaaatgttg
    20521 aagatgatct tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta
    20581 agtgagatca tggacggtac cgtatcgatt cgtgaaagga tcggacgaag caaatatatc
    20641 ccggaaacag aattagaaaa actagatcaa attattggtg aaatcaagca aacgatgcaa
    20701 acgatcgttt cagaaggagg catgagcaat gattaaagaa tatcgtacga tcggtgaagt
    20761 cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga
    20821 agtccgtatg caaaatggcg agattcgtcg tggacaagta ttggaagtcc aagaagataa
    20881 agcaatggtc caaatattcg aaggaacaag tggtatcaat ttacgtgact cttctgtacg
    20941 atttttaggc catcctttag aactaggtgt atcggaagac atgatcggtc gagtattcga
    21001 tggtttggga cgaccgaaag ataacggacc agagatcctg cctgaaaagc atttggatat
    21061 taacggagaa gtaatcaatc cagtagctcg ggattatcca gatgagttta tccaaacagg
    21121 tatctctgca atcgatcatt tgaatacatt agttcgtggg caaaaattac cagtattttc
    21181 tggctctggt cttcctcata aagaattagc tgctcagatc gcgcgacaag caacggtact
    21241 cgattcatca gatgatttcg ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc
    21301 tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat
    21361 gaatttagca aacgaccctg caatcgaacg tatcgctact cctcgtatgg ctttgactgc
    21421 tgcagaatat cttgcttatg aaaagggaat gcatgttttg gttatcatga cagacatgac
    21481 caactatgca gaggcattgc gagagatttc tgcagcacgg cgagaagttc caggaagaag
    21541 aggatatcct gggtatcttt acacgaactt agctacttta ttcgaacgag caggaagaat
    21601 ccgcggacta aaaggatcgg ttactcaaat tccgattcta acaatgccag aagacgataa
    21661 aactcatcca atccctgact tgactggtta tattacagaa ggacagatca tcttgactag
    21721 agagctttac aagagtggga ttcagccacc aattgatgta ctcccgtctc tttcgcggtt
    21781 aaaagataag ggaactggac ccggaaagac aagagaagac catgcagcta cgatgaatca
    21841 gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga
    21901 atctgcttta tctgatgtag acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga
    21961 atatgtcaat caaggattct acacgaaccg ttcgattact gaaacacttg atctagggtg
    22021 ggaactgcta tcgatgttgc caagaaccga attaaaacga atcaaagacg acatgctgga
    22081 caaatatctg ccagaaggga agtgagttct gatggcacga ttgaatgtga atccgacccg
    22141 catggaattg actcgattga aaaaacagtt gacaactgcc acaagagggc ataaattatt
    22201 aaaagataag caagatgaac tgatgcgtca attcatcctg ttgattcgaa agaacaacca
    22261 gctgcgtcaa gaaatggaag agtctatgac agaagcaatg tctgattttg tattggcaaa
    22321 tgcttcgatc acggaacctt tcatcgaaga attatttgtt ttgccggcgg aaaacgttga
    22381 tttgtctatc gtagacaaaa atatcatgag tgtaaaagta cctgtgatga attttgaata
    22441 tgatgaacag ctgacagaca cacctttgga atacgggtat ttgaattcca gtgccgaact
    22501 ggatcggtca atcgatcggt ttactaccct gcttccaaaa cttttggaac tgacagaaat
    22561 cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc
    22621 attagaatat atgacgatcc ctcaattaga agaaacgatc tattatattc gtatgaagct
    22681 ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac
    22741 tgactaagtg gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag caaacaaata
    22801 aagaaacgtt tgccggctgt tcagcttctc gcattaggat tttttctcct gattttagtt
    22861 ggcggcggcc tacttaccct accatttttt agtaatagtg ggcatgggac aaattttatc
    22921 gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac attgaataca
    22981 gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc ttttgattga gatcggcggt
    23041 cttggtttta tgatggttcc gattctatgc tttgcattga taaagaagaa agtcagtctc
    23101 agcacacgta ttatattaaa agaagcgctg aatctagaag aagtatcagg tgtgatgaag
    23161 ctcatgcttt acatcctaaa attggccttt gtgatccaat tgattggtac ggttgcttta
    23221 tcttttgtat ttgtgcctga atttggctgg ttgaaaggta tctggtacag tttgttccat
    23281 gcaatctcta gtttttgtaa tgcagggttt gatttgttag gagacagttt agccaatcac
    23341 cagcaaaatg tttatttgat catggtggta tctgctttga tcatttctgg tgggttaggc
    23401 ttcatcgtat ggcgtgattt actggaatat catcagaaaa agaagatgac gattcattca
    23461 aaaatcgcat taaccgttac aggtaccctt ttattaggcg gattcgttgt gttctttttc
    23521 acagagcgca atggggcaac gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc
    23581 ttttttatga gtgtgacacc tagaacggct ggatattatt cagttgatta cctccatatg
    23641 agccatgcag gtctgatttt aacgatgttt ttgatgtaca tcggcggaac atctgggtcg
    23701 acggccggcg gattgaaaac aactacgtta ggtgtgttgc tgatccagat gcgttcgatg
    23761 ttgaaaggac gtacgagagc ggaagcattt ggccgtacca tccgacaagg cgcagtgttg
    23821 cgggcactta ctttattttt tgttacgttg acgttgtgtg tagtcgccat catgatttta
    23881 tcggtgactg aaacgatacc taaaacttct ggcatcgaat atattgcttt tgaggtcttc
    23941 tctgcgtttg gtacagtagg tcttacgatg ggattgacgc cagacttgac tgcaataggg
    24001 aaattgatca ttatagcgct tatgtacatt ggacgagttg gtgtattgac tgtggtcttc
    24061 tctctgatga caaaagcaca aagaacacaa tcaaaataca aatatccaga agaaagtgtc
    24121 atgattggat aatttcatct tctgacagaa acctagtgat ttagtaaaat aagaaaataa
    24181 aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat tttttgaggc
    24241 actacccgtt gttgaacctc gggtagtgcc tcttttttat tgaaaggaat caataagatt
    24301 tgattccttt atttatcaat aggttataat tcatattttt ttattttttc ttttacgata
    24361 cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg aacgaatgca
    24421 tctggttgcg cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc gttagcaaga
    24481 aagtcattct caattatctt agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa
    24541 gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg attggctctt
    24601 tttgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg gaggatttga
    24661 gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat tgcttgttct
    24721 atcttgcttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc agaacaaata
    24781 aattttgtca ttccagctaa gtagattgtt tggttaggta ataaattttg gaaaggagga
    24841 atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc aataacccat
    24901 agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac
    24961 cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt ttttctacat
    25021 tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg aacgatctct
    25081 aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt
    25141 ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg aacaccggaa
    25201 acaatagata gagattctaa ggacggaatg actccttgag tttttaacca atttgctgca
    25261 gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc aacaagattt
    25321 gggttattac tagttgattg aataaacggg acaacgaatt tattacattc ttcaaaagaa
    25381 ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat ggtgatggac
    25441 tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag caaacctttc
    25501 tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa ataatcagct
    25561 agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa aatatcttgt
    25621 tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt gtctggtcgc
    25681 caagttaacg ggtaattgtc ataagaatta ataggcattt tctccactcc ttaaaattgt
    25741 ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt tgtgaggagg
    25801 gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag ttttgaagaa
    25861 gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat cagccaaaat
    25921 cctcgattgg tggaaatttt acctgacgga aaaggcaaaa atatgtttgc gatgatgcct
    25981 gcttatttgg gggagaacag atattttggc acaaaagtca ttaccgcttt tccggataat
    26041 catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc tcaaaacgga
    26101 ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct
    26161 gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt aattggagct
    26221 ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat cactacagtg
    26281 tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc taaaaaacag
    26341 tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg tccagcggaa
    26401 atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa
    26461 gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat aagtacatcg
    26521 ttgatggaaa cttctcaaat ctatgtagat gattactcag ctgccttaac agaaagcggg
    26581 gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt tggtacatta
    26641 ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc gacgattttc
    26701 gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta taacaaatta
    26761 gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt gcagatgagg
    26821 tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca gaaggtacga
    26881 tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg aagaaaaacc
    26941 aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt ggtcacgcga
    27001 cattagcaac agcatttgtt ttatttaact attacaaaat acctgatgaa acgattaaat
    27061 tttctactca aagcggtaat ctatttgtga caagaattaa agattattat tacatggatt
    27121 ttcctagtat tatgccgaaa aaagtgccga tattgtctga atatgaagaa gcgattgggg
    27181 ctaagattaa agaagcctac ttagcacgtg atttattttt cgtattagaa gatgaaaaaa
    27241 cggtggctaa attgcagcca gattttacag ctatcaaaaa ttttgaatta ggaataggag
    27301 tcatcgtgac agcagaaagt gtgcaaaaag actttgtttc tagaaccttc tttccaaaat
    27361 taacaatcaa tgaagatcca gtatgtggat cagcacattc taatcttatt ccttattggg
    27421 cagaaagaat gaataaaaca aaattgtcag cttatcaaat gtcatcaagg ggcggggatc
    27481 tagaatgtga attaaaagaa gatcgtgtga taataggagg aaaagcagtt ttgttttcta
    27541 aaggtacctc gtttatttga tttctttcaa atgaattaaa aataaattgg tggagagtgg
    27601 caaccttata gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa
    27661 gtttgcgtct cattttaatt attggtgttt gttttttatt caatcaagag taaaatgccc
    27721 catactaact atcctcggat gacttcgatt ttataaccat cagggtcaat gacaaagtag
    27781 taagaaggcg cagtgccagg taatcctttt aagtcagtaa tctcaagacc agcagcttta
    27841 tgttgctcgt gtaatttttc tagatcatca gctgctatag cgatatgacc atagccgtct
    27901 cctagatcat acgcaccatg atcatagttg taagtcaact ctaattcata gtcatcccca
    27961 ggaagtgtca aataaacaag tgtaaatttg ttttctggaa aatcacggcg gcggctttct
    28021 tcaaaaccaa atgccttttg ataaaattcg attgatgctt caagatcttt cactcgaaca
    28081 catgtgtgag ccattttcat agattcatct ctccttttcc ttttactcaa tcataacaaa
    28141 aagaagtaag gaaatagttg atttgtgctt gttcatgaga aatttctcat gtaaaatgga
    28201 tagaaaataa aactgttaag gtggaaacta ttcatggata aaccaatttt tggtgaaaaa
    28261 aagcaatttg ctgattatca aacccgttat gcagcatata ttgtcataga aagagatcag
    28321 cagattgcag taatcgaggc tccaaatggt gctttttttc ttcctggcgg ggaaattgaa
    28381 ggagaagaga cgaaagaaga agcgattgtc cgtgagctga tagaagaaat gggaattgtg
    28441 gcagaaattt cttcttatct gggagaagct gatgagtatt tttactcgaa ttatcgtgca
    28501 acttattatt ataatccagg atatttcttt gttgctgatt cttggaagcg tataggagaa
    28561 ccaactgaaa agaccaataa aatttggtgg gtgagccctc aagaagcgct tgtgaaattg
    28621 aaacgtggta gccaccgatg ggcagtcgag agatggatcg aaaaaaataa aaactgaaag
    28681 aaagcgagta gatcagcttt ctctcagttt ttttatttat tacagtacat attttttgat
    28741 tgcttcagct acaccgtgct cgtcgtttga tgaagtgatg acatttgctg cttctttgac
    28801 aagagaaacg gcatttccca ttgctactcc tagaccagca tattcaatca tggataagtc
    28861 gttctcgtta tctccgatag ccatgatttc atcttgatca ataccaaggt gctgtgcgag
    28921 attcgcaact gccgcacctt tacttgcttc tttatttagt acttcgaagt aatagggcgc
    28981 acttttgacc gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc
    29041 taaaatttct ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc
    29101 ttcaggtgtt cgatatttta gcggcatctt gactaaactt gcttcgtgga tcgtataagc
    29161 gctgatatct cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc
    29221 gacacggcgt gccattactt cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc
    29281 gatgatttta cctgatttcg tttgttgtac gagtgccccg ttataagtaa taacgtaatc
    29341 attatcttga aaaaggttca actcgtttaa ctgttcctgg acacctggta agggacggcc
    29401 agtgcataaa acgatcgaaa caccttttgc aacagcagcg gcaatcgctt cttttacttc
    29461 ttttgttact tctcttttct catttaataa agtaccatcg atgtcgatag cgactagttt
    29521 gatagacata atttcctcca atatatttta atttatttca atcaatgcgc cattacggat
    29581 atggctggca aattcttgat aagtttcgtc aaataaatca taatgatcac gcaaggacgg
    29641 atcaaccatt tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc
    29701 agcaacaagc tgactaacct gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc
    29761 gatttgtgtg cgatgtctgc cttgttctgg gcgataaaaa tcataaggta gatcgtagct
    29821 agaattcaca gcagtgtaat aaacaggatt atatccgacc ttttctacta acaaagtgag
    29881 ttcttggact aattttgaat cccggttgcc agaaaaagta gcagatttca gtggtttacg
    29941 attcaaaaaa cgtttagcta aatcatttaa aataggatca gcagattcag accattgagt
    30001 aaagtaagtg cccaagaccc catcatcaag tttgaggtat tcttctaaag taaagcttcc
    30061 gtcaagaaat ggcagaagta attgagaatg gagttcaaaa ctgctggcat cttcttgata
    30121 caactcgtgt gcgcgatgaa gcagatgctc caaaattact tccataccac gggaaacagg
    30181 atggaagtac acttgcacat acatttgata tcggctaacg atataatctt ccactgcatg
    30241 catgccactc atggaaaaag cgatcccgcc ttcataagga cgaatcacac gtaatattcg
    30301 ggtaagatca aatgttccat attctgttcc agtgaaataa gcatcccgaa gcaaatagtc
    30361 cattcgatct gcatcgatct gactagaaat catttggaca acttgtgggt tggggtaagt
    30421 tttttggatc acactagcta ccttttcagg aaaaccttct tctactcggt taagaatctg
    30481 atacacttct gtttctggag aagtgatgat ctctacagtg atggcttcat gattcgtatg
    30541 gaagatatgt tcaaatgtat gagagtaagg accatgaccg acatcatgca acagtgccgc
    30601 acataaagtc accagccgtt cctgatcatc ccagcctccg ttgcctattt tttcttttga
    30661 aaaatttcga gaaaagatat cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg
    30721 agtaaaacgg ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat
    30781 gcgtcgtaga cgttggactt ctttcgaatt gatcaaatct aagattactt gatgctggac
    30841 atggatatag ttatggactg gatcacgaaa aactttttcc ataggcagca tttgatattt
    30901 ataaggggtt gtcataatgt agctccttta tgttattacg ttgttccatt ctctggattt
    30961 ggttgttcca ttgctccagc tggatttctt tttttagcca gtcggtagaa tcctgtaaat
    31021 cacttgcaga atacagcttt tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa
    31081 aaaggggttg gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc actgcaggat
    31141 agccatttgt accaaagtct tcttttaaag cagattgata aaatcgcttc atgacttgcc
    31201 cacgtttttc ctgattaccg ttgacactta agtacatcat aacagaaatc ccatttttga
    31261 tccgacgctg agcgataccg gcaaattttt tgccatggat gcttaaatca taagttccag
    31321 gacaataaga atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag
    31381 tcaataggag catttgctcg taagcgtcat ctatggttat tttttttgtg tcactttggg
    31441 ggaaaatcaa agaaatattc aaaattcctt catctgatac gatccctaat ccacctgaat
    31501 ttctgatcac tggagcgtaa cctgcatctt ccagtgtttg gatcccgtca cttagaaaag
    31561 gagttcgaac atccttcata cctaagatga ctgtgcagtc atattgccag aaatgtaaaa
    31621 agatttgctg gtgttcttta gaatattccg ttaatatatc tgttaatgca aaaggggaaa
    31681 agtcgttttt ttgataaatg tgctgatcta gtcgaatcgc agggttcatt caaccggctc
    31741 ctttgtataa attcatattt tctacaatat tatactctaa aatcaaagag ttgttttgga
    31801 aaaaaggttt tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa
    31861 tgtagctatg agaagtaaag gagaatgaat aatggaatta tcacaaggaa caccgatttc
    31921 aatcaaattg cgtacaaaag taaaacagca aggggaagtg caagattttt attttgattt
    31981 gaaagggcaa atggtgacaa ttggcgatac gctgtacata cgttataaag agattcagga
    32041 ggagacgggc gatgagattc ctgtaacgat caaattagtt ccagatggtc atgtacagct
    32101 gatccgtgca ggagaaatgc ggatgcgttt gaaatttggc tataaagaac ggttagaaac
    32161 atcctatcga acaccatacg gtatgatcca gattgctact tttacgaaag aactgcatgt
    32221 tagtttgaaa gaccgtccca cagcagggaa ggttcgaatc gattatgatc tgttcatggg
    32281 accggaaaaa atcggcgaat attatttgac cttggatttt acagcgtaaa tataaaagaa
    32341 aagtttgatt ttttgtgtct gattttgtat gataaggagt agactgtgaa aggacgtgtc
    32401 ctgattggaa attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt
    32461 agcacacgcg atcttagaac aacgtggcga tgtcatggat ttttctgatt tagctaacca
    32521 gatccaaaac tatcttggga aatcagacag tgaaattcgt gattcactcg cacaatttta
    32581 tactgacttg aatattgacg gcagctttat ctcattaggg gataatcgtt ggggcttgcg
    32641 ttcatggtat gctatcgatt cgatcgatga agaagtaaac catggaatgg acgaagagga
    32701 tgaagatact ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca atgatgatga
    32761 agacgcgatc gattacaatg acgatgaccc agaagataca gatctgacag aagaagatga
    32821 cgatgatctg tttgatgacg atgatgatga agatgaagaa atcgctgctt ataattctga
    32881 cttgcaagaa atcggagcag atgacccgac agatgatgaa gaagacttgc caggaaatat
    32941 cgaagaagat ttaagtatca tagacgaaga cgatgatgat gaagattatg aagaggaaga
    33001 attttctgac tctgaaaaag aataaacatt attttcttac gagaggttgt gacagagacg
    33061 atcagcctat cagaataagg aggtgtgaca cgat
//
LOCUS       DAFGFZ010000018        31809 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000018 DAFGFZ010000000
VERSION     DAFGFZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.018"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            92..736
                     /gene="pcp"
                     /locus_tag="LR726_000699"
     CDS             92..736
                     /gene="pcp"
                     /locus_tag="LR726_000699"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL6215808.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            815..2092
                     /locus_tag="LR726_000700"
     CDS             815..2092
                     /locus_tag="LR726_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215809.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(2276..3025)
                     /locus_tag="LR726_000701"
     CDS             complement(2276..3025)
                     /locus_tag="LR726_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL6215810.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            3355..3735
                     /locus_tag="LR726_000702"
     CDS             3355..3735
                     /locus_tag="LR726_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL6215811.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            3831..4340
                     /locus_tag="LR726_000703"
     CDS             3831..4340
                     /locus_tag="LR726_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215812.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            4417..4596
                     /locus_tag="LR726_000704"
     CDS             4417..4596
                     /locus_tag="LR726_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215813.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            4621..5214
                     /locus_tag="LR726_000705"
     CDS             4621..5214
                     /locus_tag="LR726_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6215814.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5261..5776)
                     /locus_tag="LR726_000706"
     CDS             complement(5261..5776)
                     /locus_tag="LR726_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215815.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5802..6671)
                     /locus_tag="LR726_000707"
     CDS             complement(5802..6671)
                     /locus_tag="LR726_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL6215816.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            6797..7477
                     /locus_tag="LR726_000708"
     CDS             6797..7477
                     /locus_tag="LR726_000708"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL6215817.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7630..8610
                     /gene="pta"
                     /locus_tag="LR726_000709"
     CDS             7630..8610
                     /gene="pta"
                     /locus_tag="LR726_000709"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL6215818.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            8733..9206
                     /gene="tsaE"
                     /locus_tag="LR726_000710"
     CDS             8733..9206
                     /gene="tsaE"
                     /locus_tag="LR726_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL6215819.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            9199..9723
                     /locus_tag="LR726_000711"
     CDS             9199..9723
                     /locus_tag="LR726_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6215820.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(10213..10746)
                     /locus_tag="LR726_000712"
     CDS             complement(10213..10746)
                     /locus_tag="LR726_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL6215821.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(11610..12362)
                     /gene="xth"
                     /locus_tag="LR726_000713"
     CDS             complement(11610..12362)
                     /gene="xth"
                     /locus_tag="LR726_000713"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL6215822.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            12475..13395
                     /locus_tag="LR726_000714"
     CDS             12475..13395
                     /locus_tag="LR726_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL6215823.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            13468..14403
                     /gene="murB"
                     /locus_tag="LR726_000715"
     CDS             13468..14403
                     /gene="murB"
                     /locus_tag="LR726_000715"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL6215824.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            14701..16179
                     /locus_tag="LR726_000716"
     CDS             14701..16179
                     /locus_tag="LR726_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215825.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            16456..18516
                     /locus_tag="LR726_000717"
     CDS             16456..18516
                     /locus_tag="LR726_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL6215826.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            18599..19168
                     /locus_tag="LR726_000718"
     CDS             18599..19168
                     /locus_tag="LR726_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6215827.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(19343..20149)
                     /locus_tag="LR726_000719"
     CDS             complement(19343..20149)
                     /locus_tag="LR726_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215828.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            20287..21801
                     /locus_tag="LR726_000720"
     CDS             20287..21801
                     /locus_tag="LR726_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL6215829.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(22204..23187)
                     /locus_tag="LR726_000721"
     CDS             complement(22204..23187)
                     /locus_tag="LR726_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBL6215830.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(23192..23839)
                     /locus_tag="LR726_000722"
     CDS             complement(23192..23839)
                     /locus_tag="LR726_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL6215831.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(23924..24556)
                     /locus_tag="LR726_000723"
     CDS             complement(23924..24556)
                     /locus_tag="LR726_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL6215832.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(24572..25123)
                     /gene="coaC"
                     /locus_tag="LR726_000724"
     CDS             complement(24572..25123)
                     /gene="coaC"
                     /locus_tag="LR726_000724"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL6215833.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(25120..25857)
                     /locus_tag="LR726_000725"
     CDS             complement(25120..25857)
                     /locus_tag="LR726_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL6215834.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(25854..26843)
                     /locus_tag="LR726_000726"
     CDS             complement(25854..26843)
                     /locus_tag="LR726_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL6215835.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            27101..27643
                     /locus_tag="LR726_000727"
     CDS             27101..27643
                     /locus_tag="LR726_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6215836.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            27660..28745
                     /locus_tag="LR726_000728"
     CDS             27660..28745
                     /locus_tag="LR726_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6215837.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            28757..29563
                     /locus_tag="LR726_000729"
     CDS             28757..29563
                     /locus_tag="LR726_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215838.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            29564..30391
                     /locus_tag="LR726_000730"
     CDS             29564..30391
                     /locus_tag="LR726_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215839.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            30388..31461
                     /locus_tag="LR726_000731"
     CDS             30388..31461
                     /locus_tag="LR726_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6215840.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT        10513 a         5636 c         6405 g         9255 t
ORIGIN      
        1 aaactattgt ttcctttatt tacgctgttc aaggaaaatg gtatgataaa agataaccat
       61 atgttaaatc aatcggacgg aggaacaaca gatgaaagta ctagtaacag gttttgatcc
      121 atttggtggg gacaaagtga atccagccta tgaagcagta aaaaaaatgc cagatgaaat
      181 cagcggtgct gaaatcatca aagtggaagt accgaccgta ttcgaaaaaa gcagtcaagt
      241 agtcaaagaa gccatccaac agcaccaacc agatgtcgtg atctgtgtcg gacaagcagg
      301 tgggcggtca gcagtctcgt tcgaacgtgt cgcaatcaat ctagcagaag caagaatccc
      361 tgataatgaa ggaaatcagc cattcgatac ggctttagaa gaaaacggtc cagccgctta
      421 ctttacctca ttgccaatca aagcaatgac aaaaaatgta caagatcatg gtctaccagc
      481 gtacatctct tatacagcag gaacatttgt ctgcaatgat atcatgtatc gtttgcttca
      541 tttgatcgaa acacaattcc cggcaattcg aggtgggttc atccacgtgc cattctcacc
      601 cgatcaagtg atcgatcgtc cagtgggtac gccttctatg tctttagaag acattgcttc
      661 ttctttgact tatgcagttg aagcagcagt agaaaataaa gaagatattt ccggaaatgc
      721 cggaacaact cactaattta gcaagaaaca ctggctggtt gctaacgtta tgttagtagc
      781 cagctttttg taattagaaa ggaaagaaac agccgtggaa gaatggcaat ccgtctttga
      841 agaatggttt ccaaaagaaa tcagcaaaag ctatcccatc aaaatttcta aacaatacac
      901 aagcagtcaa cgttgggaga tctatgcaaa attaacgaaa aaacagcggg aactagtcga
      961 taagcaccgc cgatatctaa tcagctctcg ttttatggaa gaacattatt tagctgcaac
     1021 agattgggtg ttttctgact tcaagatcaa tccgtttttt cgaacaaaac gaagccagca
     1081 aaaattgtat tgtgagtgtg gtagagaatt aaaagtgcaa tacatcgtta aatcgccaaa
     1141 aaccggaaag atcctaaaac taggcatcaa ccactttgcc gatcacctgc atgtctctcc
     1201 gacagtggca gcttcgatcc atcagggaat gacaaaagtt gatctggcct tagacgaatt
     1261 attatggctt aagcagaaga atatcgattt tcctgaagga ctatggcaaa aatactgttt
     1321 tgtcttgtac caaaatcgtc ggatgaaaca gccatattta ccggatataa aattagctca
     1381 gcggctcgca gaatttcgcc aagtagaaat gccgatttac atagcggatt atcaagcatt
     1441 ggaaaatgaa atcaagaaaa taagtgaaca catcaatggg cagtcaaaaa agcgacagat
     1501 aaaaaaagaa ctatttgacg actttgcaga agaattagta aaggatgtag aagaattctt
     1561 gatcaactat cgcgcatttt tacgtaaaga ttggcaatcc atcgtatatg aagaagtgcc
     1621 agtacatcct aatgcttatt ttgaaacatt tatttccgtg ttgagaaaga caaaacgtca
     1681 acgaacacca gaagtcactg ctcagatgga atattttgcg aaaaaccaac gattcatcca
     1741 accaaaaatc tatctattta tttggaagca atattgtcgc tatggtttta cagaagggtt
     1801 cttcgacagc attcctcgga tagtcagaaa tggtttttta aaagtattgc gtaaagaacg
     1861 agaagctata caatctgctg ataagaaaga ccgtacagtc tcaaaagaaa aatggcaact
     1921 agtagtgaaa gatatccaat ctggaaatgt gcaagagaca atcgacaaat ggaaaggaaa
     1981 acattatcgt tttactgaag ctcagaaaca agcgttggaa tattatcaga agttagaaga
     2041 atcactgcgt ttcaatgatg aggcaagaaa gtatctgaag gaattactat aaaaaataga
     2101 ggctgggaca gaagcgttta actccaagaa ataagaagaa attcacgaaa attgcttttc
     2161 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgattccg ccgtttatac
     2221 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctcag tttttttatg
     2281 cgtgttgttg tgcgttgtag tattcttctt ccattttttg catacgttca cgttcttttt
     2341 ggttatacca aggtgaaacg gtaaatgcaa ggacatcatt cagcaagtaa acagagctgt
     2401 tcacgaacat cgccaaactt gcagatccat gcgtaaatga aatataccaa aggaccattt
     2461 gagcaagccc tgaagctaac caccagtaat attggttgtt gtaacgcaag aaactgatga
     2521 taccagcagt caagctaata gaaaaagcaa tggcatctac ccacggccga ggatcatctg
     2581 tataacgacc aatcaggaat cctgaaacaa gatagacaag gaaagtactg atgattgcga
     2641 taaaccagct ttttccagtg aattttcgga tttttgaagc catattatga ttccattctt
     2701 tacttaacaa tacggggata tccaatgtga tcatataagc aatctgttca ccgatactta
     2761 aatagttctt tgctgaaaac ccaacgataa taaaacaaat cgcagacaaa atacctaaaa
     2821 taccgttgac tgattttgcc gcattgatgg ataaaataca caatacacca agcgtggtac
     2881 cgataaatgt aataacggac agccaagtga tgggactatt gacaagagtc atgacctggc
     2941 agccaagact gaaaaagaaa agataataat tttgttgcgg ccagcctttt agctgatgag
     3001 ctaaaaactg aaaatatgac ttcatgtatg aggactcctt aagacactaa atgtagtgtg
     3061 ttctgtattt actaacacaa tctattgtat gcttttcttg aaaaaaaact agtctttcaa
     3121 ttataaataa tttgtgttat gataattttc tgtttgtttt cttgaaaata taatttctta
     3181 ctttcattta ttatattggg aacaggaaga gtgtcttgga ggacctactt tctgtttttt
     3241 ttgccttttt tcttataaaa aggaaaaaca gctatttttt tcaatctgac acatttacaa
     3301 tggaactgga gattaatata gatccgagct taaacgaaga ataggagtgc tcatatgaac
     3361 cttacaacga tccatcatat agccatcatt gtttcagatt atcagaaatc acgtgatttt
     3421 tatgttaata aattaggatt tgaagtaatc agagaaaatt accgcaaaga acgagatgac
     3481 tataaattag atctgaaact aggaagtgct gaattggaga tttttggaat tgctaatgcg
     3541 ccacaacgac caaactatcc agaagcttgt ggcttgcgtc atcttgcttt caaagtagac
     3601 gatattgaag aaacaattgc agaattgcag tccttaggga tcgaatcaga accgattaga
     3661 gaagatacct ttacaggaaa aaaaatgacg tttttctttg atccagacgg attgccatta
     3721 gaactgcacg aataaagata acaaaattgt cacttttttt tcgtttcatt cgatttttca
     3781 ttcgttcatt tcttgatatt ctttaaaaaa aagaaacagg agaatgaaga atgaaaaaaa
     3841 tgatgtttgg tgttgctgcc ctcatcgtaa tagggattac cggtatgacg ggactgctca
     3901 atgccgcttt tcacatggat gatattagtg accaggaata tcggatctca ttgaaaaaag
     3961 cagcagcaat cgtaaaaaac gaaacaaaag aacataacat atctgctatt cattttgcag
     4021 ttgaaaaaga tttggagaat cctacgaatg caacatatga ataccagttt ttttgtgaag
     4081 gacaagtcat agcagtcaat ccatccactg ggaaaacaac cgtacgaaat gacacggaaa
     4141 aggccgcaga acatcttttt gatatagaaa aaacggaaga gttcaaacaa ccgcaagcag
     4201 cgatgaagga agcagtcagc tactacggga aaaaagcaaa agtgaaagag tggacacttc
     4261 tagtcaaaga tgaacgtcct tattataaag tccgattaat agatggagaa caggaccgtc
     4321 aggtatggat atctgcatag aagaaggata ttgcttctct tttttacaat ataagagtaa
     4381 gctaagagag taaagatgaa aggaagttga gataacatgg aaaaaatgaa atgccctaat
     4441 tgcggaaaaa aatttgcata cgaagaagtc aataatgtag tcgaacataa tgataaagaa
     4501 atgccagtcg tctgccctta ttgccgaact gaagcggcaa gaatcgttac tcatggttat
     4561 ttcattactg aaaagattga agatttttta aaataacgat tttgcgattt ctttttctgc
     4621 atgttagaat ggagaaaagg agaggaaact atgattcgtt ttgcgagaaa agaagatgct
     4681 ccagaaatcg ccccattgat cttagtgatt ctaaaagata tggaactgcc atttttgaag
     4741 cagacagggg aacaaaagac tttagagatt ttagaagaag caatcactga tccaaattat
     4801 cgttacagtt attcacgagg tatcgtagaa gaaatagacg gtaaagttgc aggagttgct
     4861 tacggttata cagatgaaga agaatcaatc atcgatcagc cactggtacc tatcttagag
     4921 aaatatcagc tagatgcgtc tacacgtttg ttcattgata aagaaacgtt tcccaacgaa
     4981 tggtatttgg actctatcag tgtatcagaa gattttagag gacaaggcat cggttcgcga
     5041 ttattagaag cattacctaa attggcgaag aaagctaatc gctccgtcat cggtctaagt
     5101 gtggatgaaa aaaatccaaa ggctaaaaaa ttatacgaac gccatgactt caaagttgtc
     5161 ggtcagcgga tgatcagtgg tcatctttat gatcatatgc aaaagaatat ctaaaaagat
     5221 gtacctgaaa tgcgtatttc cgcatttcag gtacatcttt ttaacttaag ttaagtcggt
     5281 aagaaacagg cttatcgcga cttttgtctt tacggtagag ccagtaatct aaaaccgcag
     5341 caatacaaat acaaactgga gcatcttcat catttgagac atccaataca tagtaatctc
     5401 ctgaaaaaag tgttgcttta tccattttca tgatttgctg attgaaatga tggatttgat
     5461 attgatgatt atagatatca ccttggactg tccagtgaag tctacgaata taataaaagt
     5521 caccgggcca attgaatatt ttctgcatgg ttcccacctt ttcgaactct ttatacagct
     5581 caaaacgcgt accgaatgtc aggcttatct gcttgatgtg agcaacgaga tcgccattca
     5641 tggcataaag agacaatgca tcgccctttg ttccccagcg accaaccatc aaaaacaggg
     5701 aatgtccttt ttcgtttttg acaattgtcc gagttgcacg tccaagctgc ttttcctgaa
     5761 tgaaaaattc tgacatctat atccctcttt tctagataaa actacaagtc ttctgcaatc
     5821 gcgcgcaata tggcttttcc tacgccagat tcaaggtttg tgtccgttgt atatttggca
     5881 taatctttta cttcgatttc tgcatttccc attgcgaaac tgactcctgc tacttgcagc
     5941 atgctgacat cattgaagtt atcgccaatc gtcatgactt cttctaaaga aatcccacgc
     6001 tcgtgagcga catgggcaac agcaattcct ttttgagcgt ttttatggtt cacttcgata
     6061 ttgttttgac cagaggaagt aacggctaga tcgcctaaaa gagaaatctg ctttccaact
     6121 ggacctaata cagctggccc ttctgtatga aaacaaataa tttttaaaac ttccagattc
     6181 ttttcttcca aaatagcacg aatactgtcc acgtatgtga tgtgcaataa ctccagctta
     6241 gctgaggcca tggcaatcgc cattttatac gtaaggtgag gtaaatgggt agccaccatg
     6301 gaagcaaaac tttcaattcg tttcgcttga ttttcagaat aaagaccatc gttggttgct
     6361 acttcatagt aaataccgtt agcatctaga atatctaaaa cagagattgc ttgattattt
     6421 ggaatcgaca cggtaaacaa tgaatttcct tctttatcaa atacctgtgc cccgttcaaa
     6481 gtgatcatcg cacaatcgat tcctacctca tctaaagctg cgcgagcttc ttgatagttt
     6541 cgaccagtag cgaccatgaa ctcaacaccg gcatcatttg caaaacgtat cgcctcggca
     6601 ttttcaggag aaacactcat atgggcatct aataatgtgc cgtccatatc tgatgcaatt
     6661 aatttgatca taatgattat cgttcctttc tgcttcttgt tcctagttta ccatattaat
     6721 caagtgctga gggtgaagat tgtcccttgt gattgaagaa aatgtgagtt attcttttta
     6781 ttaggaggtg gagtttttga aagaaatcat tcataacagt tggcaagaag ttttgtcctc
     6841 agaattttca aaagattatt atctgcatct aagagaattt ttaaaaaaag aatatgcgtc
     6901 ccaaaagatc catccagata tgtatcatat ctatgaagca ttggaattga ctccttatga
     6961 agaagttaaa gtggtgattt taggacaaga tccttatcat ggagaaaatc aagctcatgg
     7021 cttatctttt tctgtgcaac ctggcgtaaa gatcccgcca tcgttaagaa atatctataa
     7081 agagctttat gatgatcttg gaatcgcacc tgttcaacat ggaaatctag tgtcatgggc
     7141 taaacaggga gtccttttac tcaacaccgt tcttaccgtt cgggaagggc aagcgtattc
     7201 tcatcgcggg aaaggttggg aaagattaac agacacgatc attgaaaaat taaatgaacg
     7261 ggaaaaacca atcgtgttta tcttgtgggg aaaaccggca caagaaaaaa tcaaaatgat
     7321 cgataagagt cgccatatca tcataacatc tccacatccc agtccgttat ctgcaagtcg
     7381 tggctttttt ggttccaaac ccttctcgaa aacgaatgat gcgctccttg cgttaggaga
     7441 agaaccgatt gattggcaac tgcctgaaac agtgtaataa aacagcgctt tcctgttata
     7501 gtgcagttcg ctttgtatgt gaaaaactta atttttccag catttttgac tgatacagat
     7561 aaaataaact gttctttttt tctggaatag tgtatgataa tacatataaa aacgtctgga
     7621 ggtcatcttg tggaattgtt tgatagttta agatttaaaa ttgttcgccg tgggatcaaa
     7681 atcgttttcc cggaagctac tgatgctcgt attttaggcg cagctgcccg gttgaaagca
     7741 gaagaacttg tggaacccat tttagtaggg aacgaagaag aaataagaaa tgcagcgcat
     7801 gccagaggaa tcaaaacatc tagctttacg atcatcagcc ctgataatta cgataagtgg
     7861 gatgaaatgg tggatacttt tgttgaacgt agaaatggaa aggcatccaa agaacaagca
     7921 gaaaagattt taaaagatgt gaattacttt ggtacgatgc ttacgtatat ggggattgca
     7981 gacggaatgg tctctggagc gatccattcg actggcgaca ctgttcgccc agcacttcag
     8041 atcatcaaaa caaagcctgg catcagtcgg acaagcggag cgatgatcat ggtacgtgga
     8101 agagatcaag aaaaatatgt cttttcggat tgtgcgatca acgtgaatcc aactgcgcaa
     8161 gaattggctg aaatcgccgt agattctgca aaaacagcag aattgttcga tatcgaacca
     8221 aaagtagcaa tgatgagctt ctcgacaaaa ggctcagcga aatcgccaga agtagacaaa
     8281 gtagttgaag caactaaaat cgctaaaaaa ctagcgccac aatacatgat cgacggcgaa
     8341 ttgcaatttg atgcttctta tgttccttca gttgcacaac tgaaagcgcc agattcagaa
     8401 gtagctggtc aagcaacagt ctttgtattt ccagaattgc agtcaggaaa tatcggctat
     8461 aaaattgctc agcgtttcgg taactttgaa gcaatcggac caattctaca aggcttgaac
     8521 aaaccggttt ctgatttgtc tcgtggagca aatgaagaag atgtatataa attatcgatt
     8581 atcacagcag cacaaacatt gattgattaa caaatacacg cgaactagcc aaaaacagca
     8641 gctgctgttc ttgactagtt cattttttct ttaaaaaaac ttcattacgc gatatactgg
     8701 caaaaagaaa gaagagcggg agaagatgaa aaatgatcga actatcagga ttaacaatga
     8761 cagaacaatt cgcagaggca attggtcatt ccgcaatgcc aggtgacaat ctgatattga
     8821 ctggtgatct aggtgcgggt aaaacaacgt tgacgaaagg aattgcccaa ggattaggga
     8881 taacgcaaat gatcaaaagt ccgacataca cgattattag agaatatagt caaggtcgga
     8941 ttccgttgta tcatatggat atttatcggg tagcagctag cggagcagat ttagggttag
     9001 atgaatattt tgaaggtgat gggctttccg ttgttgaatg gggaaatcta ttagaagaag
     9061 cactgccgga ggattatttg gaactgattt tagaaaaaag tgatacagat ttagaaaagc
     9121 ggtatgtgaa actaatctca tacggggagc aaagtgaaac cttcaaacag cgaattctag
     9181 aaaaatgggc tgaaaatcat gagtgaagaa gttgggatca tcttgcggga agcagtgcca
     9241 ggagatgcaa aagacattct tttgatgatg ggacaagtta ataaggagac agaattttta
     9301 gttttggatg aagcagaact cctgttacca ccagaaacct tagaagaaga gttggattat
     9361 atatacgaaa gcaataataa tttattgctg cttgcaatct atgaagggac gattattgga
     9421 acagcatctg tcaaggcaga cagtcaattt cgtttgtctc acgtgggaga agtcggtatc
     9481 agtattctgc aggaatattg gggaatggga ctaggcactc ttatgctaga agaaatcatc
     9541 agttgggcga aggaaatggg tgtacttttt cgattggagc ttgacgttca agttcgtaat
     9601 gaaagagccg tacaccttta ccgaaaaatg ggcttccaaa tcgaagcggt catgcctaga
     9661 ggggcaagaa cagatctagg tgaattttta gatgtatata aaatgagtta tttgattgaa
     9721 tagaagaaat aggaaaagag tgagaaactg tcatcctttt gagaaggcag aaaaacacac
     9781 gaagggactg tgacaaaagt cgtgtcgcag tccatttttt ttgaataaac ggtgttcaat
     9841 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
     9901 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttggtaaa cggtgtccaa
     9961 aagcttgcat ctgcggtaat aagtccaacc aaatgaaaaa tatgagaagc tatttttgtt
    10021 tggctgttct tattacttga tgcagaacag ctttttcaca acctctccaa taaacggtgt
    10081 tctcctgcca gctcctcggt caccagtcac aatatttaat aaacatgaga agctattttt
    10141 tgaaattgct ccttgtgact cggagtgaaa cggcaggctc acgacctctt tgtgtgtttc
    10201 ataagtattc ttttacttta tagtcacaag ttttttcaat ggatctgtcc cgaatttttt
    10261 ttcttgataa agcaggattt ccgcacatgc acgactatct tccaatgcat catggtgatg
    10321 atctaattgg atgttcaaat tttcgcacac agtgttcaat cgatgatttg gaaattcagg
    10381 aaacaggcga cggctacttt tcactgtaca taaagaaaga taattaggct gttcaagatg
    10441 ataataatct aagcatcccg ctaagacctt tgtatcaaaa gcagcattat gtgcaacgac
    10501 taaacgattt ttttgaaaat atggttggat agtttgccag acttctggga atttcggcgc
    10561 attgcgtacg tcttcttgat ggattccgtg aatttggaca tttcgccaga aaaaaggagt
    10621 ctcaggctgt atcaatgtat agaattcatc aacgatctgg ctatttttta ccatgactag
    10681 cgcaagagaa caagcactat aaggctgatg attggcagtt tcaaaatcca tggcaacaaa
    10741 attcatttct gtaaaacctc cttgggaaat tactgtaaca tacttttgaa aaaagcaaat
    10801 aaatttaaca aaaaacatag aattttttat gtaatatttg ttgagaaaag ctagtttttg
    10861 aactcattcc ttgtgatttg gagttagatg gggatcatga cttctcggta ttaagtcaaa
    10921 tttatgcaaa gttgtgagga accattttca aaaaaatttc ttacgactcg aaagtaggcg
    10981 actttttcac aacttctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca
    11041 aaaaatagca aaatatgagg aactattttg ccatttgttt ctctaatact caggtcagga
    11101 cgctgatttc acaacttctc aataaacggt gttcaaaagc ttgcatctgc ggtaataagt
    11161 ccaaccaaac gaaaaatatg aggaactatt tttgtttggc tgttcttatt acttgatgca
    11221 gtgcagcttt ttcacaacct cttaataaac ggtgttcaaa agcttgcatc tgcggtaata
    11281 agcccaacca aacgaaaaat atgaggaact atttttgttt ggctgttctt attacttgat
    11341 gcagtgcagc tttttcacaa cctcttaata aacggtgttc aaaagcttgt atctgcggta
    11401 ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt cttattactt
    11461 gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc agtttctcgg
    11521 tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt tcttaatact
    11581 cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac tggacaatga
    11641 tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc taaatctttg
    11701 ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt gaatcggtag
    11761 ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt atcgataaac
    11821 ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc agcatttttt
    11881 tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag atccccacac
    11941 acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc ttcttcccat
    12001 tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta acaggttaca
    12061 agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg gatatcgata
    12121 cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt gccggaatag
    12181 ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa atcaatttgt
    12241 cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc atgaaaaact
    12301 tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga gataaatttc
    12361 aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt gtacctcatt
    12421 ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa gtagatgaag
    12481 attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac ttatgcgaaa
    12541 aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa agaaagaatt
    12601 caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt agaagaaaaa
    12661 atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc caaaaaatgt
    12721 ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct aaatgaagtt
    12781 cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg tttcaaccgg
    12841 cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt gatccacctg
    12901 caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga tttgttttta
    12961 catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac aaaataccag
    13021 ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac acaaaatcaa
    13081 acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata tcaagtaaca
    13141 agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca agatgctgat
    13201 ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg cgggtttgag
    13261 ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac agaattgctt
    13321 aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa agatcactac
    13381 cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa taatgaggac
    13441 gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga aagcttagcc
    13501 acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa aacaggtgga
    13561 ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat catcgattat
    13621 tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct gatcgttcaa
    13681 gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat tcatgtcaaa
    13741 ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta tgcagctttg
    13801 gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt cggtggagcg
    13861 gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc tgaagtggat
    13921 cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt attttcttat
    13981 cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt cagtttgcaa
    14041 acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt gcgtcaatca
    14101 aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt aggacactat
    14161 acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg tgcacaagtc
    14221 tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac agattatatt
    14281 gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc tttagaaaca
    14341 gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag tttagccaat
    14401 taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc ctgctctttt
    14461 ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa
    14521 tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa aaaagataaa
    14581 aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt ataggtgaaa
    14641 agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga ggtgtaggat
    14701 atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct agctagatat
    14761 ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac attaggtttg
    14821 tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac tgaattcaaa
    14881 acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg
    14941 acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc tcgactgtta
    15001 acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca ttttttaagc
    15061 atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca gcatcaaata
    15121 aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt attatttggg
    15181 gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt catgaatgaa
    15241 gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa
    15301 cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg gaatcaacca
    15361 atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat gaagatcatc
    15421 aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat tgatgagatt
    15481 tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg cgcatttgga
    15541 cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc agtggaattg
    15601 attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata tatacgaaaa
    15661 gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg
    15721 actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc aatattcagt
    15781 acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa agatgaacgt
    15841 ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg tccggtatca
    15901 tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc ttataccgat
    15961 tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga gagtcgaatc
    16021 acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa taaagaacag
    16081 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
    16141 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
    16201 tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact agatataact
    16261 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
    16321 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
    16381 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
    16441 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
    16501 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
    16561 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
    16621 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
    16681 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
    16741 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
    16801 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
    16861 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
    16921 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
    16981 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
    17041 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
    17101 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
    17161 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
    17221 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
    17281 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
    17341 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
    17401 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
    17461 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
    17521 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
    17581 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
    17641 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
    17701 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
    17761 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
    17821 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
    17881 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
    17941 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
    18001 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
    18061 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
    18121 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
    18181 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
    18241 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
    18301 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
    18361 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
    18421 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
    18481 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
    18541 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
    18601 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
    18661 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
    18721 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
    18781 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
    18841 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
    18901 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
    18961 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
    19021 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
    19081 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
    19141 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
    19201 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
    19261 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
    19321 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
    19381 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
    19441 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
    19501 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
    19561 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
    19621 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
    19681 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
    19741 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
    19801 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
    19861 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
    19921 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
    19981 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
    20041 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
    20101 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
    20161 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
    20221 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
    20281 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
    20341 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
    20401 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
    20461 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
    20521 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
    20581 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
    20641 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
    20701 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
    20761 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
    20821 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
    20881 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
    20941 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
    21001 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
    21061 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
    21121 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
    21181 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
    21241 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
    21301 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
    21361 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
    21421 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
    21481 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
    21541 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
    21601 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
    21661 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
    21721 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
    21781 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
    21841 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
    21901 ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    21961 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga aaaagagcgt
    22021 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    22081 gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
    22141 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt gatcagcatg
    22201 attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt ccatatattc
    22261 atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc gaatcttgcc
    22321 attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg ctttatactg
    22381 atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa tcaatacacc
    22441 aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc aacgtccatc
    22501 aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat ccaaaataac
    22561 atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt gttttttata
    22621 gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt ctgatcctgc
    22681 agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg ctgttcccac
    22741 accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc caagccagta
    22801 aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa catagtctag
    22861 cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg cataaccacc
    22921 tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat cacttttatt
    22981 ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt tcaaagagac
    23041 tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt tgatcagtaa
    23101 ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat ctacagaacc
    23161 aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat tcttggaatg
    23221 ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa atatccgttt
    23281 cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct
    23341 tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc aatgtgactg
    23401 gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca taatgtttag
    23461 taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca taaacagcac
    23521 tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt ttgattgcgc
    23581 catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa atcaatacat
    23641 cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca aaagagacat
    23701 acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc cctgcaaaaa
    23761 tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac
    23821 ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc attcaatagt
    23881 aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta gtaagagaag
    23941 ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga cctgcgacga
    24001 ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa acagcattta
    24061 aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc atattcatca
    24121 ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa acggaatctg
    24181 ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc accccaatca
    24241 agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct gttccgatga
    24301 ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg ctacggatca
    24361 tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct aaaacgatcg
    24421 aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata gggataaaac
    24481 ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt
    24541 tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta agagtcagca
    24601 tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa tctccacaag
    24661 ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat gtttccagat
    24721 tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca atcaattttg
    24781 gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca atgccgtttg
    24841 ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca gcttgttttg
    24901 ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct gtatggactg
    24961 cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg cttttagaca
    25021 taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca gcgcttttgt
    25081 aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt cctccagtct
    25141 aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt ccactgttcc
    25201 atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc
    25261 gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca gaagagattc
    25321 ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct gccattcacg
    25381 tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct cggtgttaga
    25441 agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat tgtccagttc
    25501 ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa aactaaaagc
    25561 aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt cttttttcgt
    25621 tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa tcgttatatt
    25681 ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg tggtatccgg
    25741 attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat
    25801 agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa tcttcattta
    25861 tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag tttagatgct
    25921 gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc aatctgcaaa
    25981 tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac agaatcaatt
    26041 ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag tttgattccc
    26101 ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag aagactgctt
    26161 agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc taaagctttt
    26221 gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag actttttagc
    26281 catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc tagtttatgt
    26341 aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac aactttttta
    26401 tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt aaaccctgct
    26461 gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac taaccagtct
    26521 cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag cccaaaacca
    26581 gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac gacagttccg
    26641 gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga ctcgctcatt
    26701 gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac ttcattttca
    26761 gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc ctttattacc
    26821 tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc ttttatcata
    26881 ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa
    26941 aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc agcaaaaaaa
    27001 attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt ggtagtaaac
    27061 aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg gtgagaagtt
    27121 aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac gcacagattt
    27181 gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa tggaaacatt
    27241 cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc aagaagcagc
    27301 gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg aagaaaacgg
    27361 gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct
    27421 gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg aaacgtttat
    27481 ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg tggcaaaagc
    27541 aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa
    27601 acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg ggtaaaaacg
    27661 tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat gaaacagtat
    27721 taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt ggtccttccg
    27781 gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct acagaagggg
    27841 acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga caagtaaata
    27901 cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac gttgcttttg
    27961 gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc
    28021 gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa
    28081 gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg ctattagatg
    28141 aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa ctgcgggatc
    28201 tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa gaagcgttgg
    28261 caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt ggtacacccg
    28321 tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg gagagcaata
    28381 tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag caatttgaat
    28441 gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc ccagaggatt
    28501 tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc
    28561 gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg atggtccatt
    28621 caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca gaagatatcc
    28681 atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac agctacgaag
    28741 aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt atattttgtg
    28801 gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt tttttgatat
    28861 gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga catcgctttc
    28921 tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc tgatcatcag
    28981 ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc tgatgctgat
    29041 tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg ggattttcag
    29101 tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga aacagatatt
    29161 attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat ttatgattat
    29221 gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta gtagagattt
    29281 aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat taaatggtgt
    29341 gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc tgactcgtct
    29401 gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt tcttggttac
    29461 acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta tgttcatcgt
    29521 gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg
    29581 gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct tctatctgat
    29641 tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta cttgggaaaa
    29701 ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata cgttcatgct
    29761 ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta tgggaatcta
    29821 ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca tcttattagt
    29881 ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac taggattcgg
    29941 attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa tcgtcgtctt
    30001 gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct
    30061 tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt ctccaggaat
    30121 catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg ttacattttt
    30181 cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg caagacaagg
    30241 aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca tgatattggt
    30301 cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag
    30361 agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt tttaggtatt
    30421 ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg
    30481 agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc tagtttgatc
    30541 acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga ttctaatgaa
    30601 gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat accaagtgaa
    30661 tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca ttcgaagatc
    30721 aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc agataatcag
    30781 tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa attctttgat
    30841 gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga cagtttgatg
    30901 ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg ctattctttg
    30961 aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca tttaacacca
    31021 aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga agaagcctcc
    31081 gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga acatttacat
    31141 tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat gccaaaaacc
    31201 gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc ggaaaatgcg
    31261 gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa agcgttattg
    31321 ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat ctcccatctt
    31381 gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct attcttagaa
    31441 ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta tcaacgttct
    31501 aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc aaaagcggat
    31561 gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta ccagagaaaa
    31621 gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa gattgaaaaa
    31681 gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt ctgtaatcta
    31741 ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa acataaaaat
    31801 catattaga
//
LOCUS       DAFGFZ010000019        31136 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000019 DAFGFZ010000000
VERSION     DAFGFZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.019"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            15..347
                     /locus_tag="LR726_000732"
     CDS             15..347
                     /locus_tag="LR726_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL6215841.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            938..1267
                     /locus_tag="LR726_000733"
     CDS             938..1267
                     /locus_tag="LR726_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215842.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            1254..1595
                     /locus_tag="LR726_000734"
     CDS             1254..1595
                     /locus_tag="LR726_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL6215843.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1709..1948
                     /locus_tag="LR726_000735"
     CDS             1709..1948
                     /locus_tag="LR726_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215844.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2015..2254
                     /locus_tag="LR726_000736"
     CDS             2015..2254
                     /locus_tag="LR726_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215845.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            2381..3070
                     /locus_tag="LR726_000737"
     CDS             2381..3070
                     /locus_tag="LR726_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL6215846.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            3076..4242
                     /locus_tag="LR726_000738"
     CDS             3076..4242
                     /locus_tag="LR726_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL6215847.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            4324..4995
                     /locus_tag="LR726_000739"
     CDS             4324..4995
                     /locus_tag="LR726_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL6215848.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5048..7024
                     /locus_tag="LR726_000740"
     CDS             5048..7024
                     /locus_tag="LR726_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL6215849.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            7037..7789
                     /locus_tag="LR726_000741"
     CDS             7037..7789
                     /locus_tag="LR726_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL6215850.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7805..8566
                     /locus_tag="LR726_000742"
     CDS             7805..8566
                     /locus_tag="LR726_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215851.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            8579..8839
                     /locus_tag="LR726_000743"
     CDS             8579..8839
                     /locus_tag="LR726_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215852.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8836..10926
                     /locus_tag="LR726_000744"
     CDS             8836..10926
                     /locus_tag="LR726_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL6215853.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(10962..13019)
                     /locus_tag="LR726_000745"
     CDS             complement(10962..13019)
                     /locus_tag="LR726_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL6215854.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(13164..13355)
                     /locus_tag="LR726_000746"
     CDS             complement(13164..13355)
                     /locus_tag="LR726_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215855.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            13543..13998
                     /locus_tag="LR726_000747"
     CDS             13543..13998
                     /locus_tag="LR726_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215856.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            13995..14237
                     /locus_tag="LR726_000748"
     CDS             13995..14237
                     /locus_tag="LR726_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL6215857.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            14255..14557
                     /locus_tag="LR726_000749"
     CDS             14255..14557
                     /locus_tag="LR726_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215858.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            14629..16683
                     /locus_tag="LR726_000750"
     CDS             14629..16683
                     /locus_tag="LR726_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215859.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            16683..19295
                     /locus_tag="LR726_000751"
     CDS             16683..19295
                     /locus_tag="LR726_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL6215860.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            19292..21823
                     /locus_tag="LR726_000752"
     CDS             19292..21823
                     /locus_tag="LR726_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215861.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            21875..22207
                     /locus_tag="LR726_000753"
     CDS             21875..22207
                     /locus_tag="LR726_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215862.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22208..22801
                     /locus_tag="LR726_000754"
     CDS             22208..22801
                     /locus_tag="LR726_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215863.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22964..24658
                     /locus_tag="LR726_000755"
     CDS             22964..24658
                     /locus_tag="LR726_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL6215864.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            25346..27223
                     /gene="ltrA"
                     /locus_tag="LR726_000756"
     CDS             25346..27223
                     /gene="ltrA"
                     /locus_tag="LR726_000756"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL6215865.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            27354..27731
                     /locus_tag="LR726_000757"
                     /pseudo
     CDS             27354..27731
                     /locus_tag="LR726_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            27731..28936
                     /locus_tag="LR726_000758"
     CDS             27731..28936
                     /locus_tag="LR726_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL6215866.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            28952..29596
                     /locus_tag="LR726_000759"
     CDS             28952..29596
                     /locus_tag="LR726_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215867.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            29607..30413
                     /locus_tag="LR726_000760"
     CDS             29607..30413
                     /locus_tag="LR726_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215868.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            30435..30665
                     /locus_tag="LR726_000761"
     CDS             30435..30665
                     /locus_tag="LR726_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215869.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT        11164 a         4866 c         6416 g         8690 t
ORIGIN      
        1 gggtggatta ttttatgtca tatacaggaa ttttgagtct cgaagatatt tgtcactacg
       61 gtaaacggtg taccgctaca gaaaagatca cgaagaaact atcaactggt caaagtaaaa
      121 ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagaa gaaatgattt
      181 attacattgg gaaacaaaaa caaataattt taaaagatcc tatcccttta aaagagctat
      241 atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga agaaaaaatg
      301 gagtcttgcg ttgtaccgca aaagggatgg ggcgtttaat cagctgaatt gatcattctc
      361 ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa
      421 cttagaatca ctagaaaaat aaggagtaga cttgatctat caacaattcc cttcggggag
      481 tgacggggta ggtttttacg attggtaacg taaaaaattg gtcaatcgag taaatgccct
      541 tgtttggata gtggtgaaat gaatttgtgt aaataatatg cacagagtaa acaagaggga
      601 atttagaagt tagacaagtt agaatagcgc ctagattaat catgagcagc tactaaggga
      661 tctaggcttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat ggggaggacg
      721 cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac gttgatcatt
      781 ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg ccataaacga
      841 gtcgatttat tttatatatt tttgagtgaa cgaaaaacga taaagatatt cgttatgaag
      901 taaaactaat ttagtagata gcaaaggtgg tagaggaatg attcaaaaat ttgcgaaaat
      961 gagtaaaact gcatggattg aaaagcttga aaatgacttg agtgtcatgc gattagctcc
     1021 aaagaaattc aaacgagaga ttgcagaaaa agaagcaatt ttgtcggtat tgaatgcggt
     1081 agacgataaa tgtccagtaa aaattagttt ttcagatata gcaacaatta tcgaaggtat
     1141 ttattatggt gaagttgatg aagagatatc ttctacaaaa gatttattag attttttgga
     1201 taaaaacaaa gataccttta tagtactaac tgattatgag gaggttacaa caaatgaaaa
     1261 attatgatcc gaatatacga ttgggcacac atacgattaa ggtttctttc caacgttggg
     1321 attacaaagg atttgtgacg tttagaagag gcggaaattg taaaggatta gatgttttag
     1381 cattagacga ggatgattta tacgatcaaa aattgactga taatccaatt ggattcggct
     1441 tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttacg gatgataatg
     1501 gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt gtcagcgttg
     1561 aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga
     1621 taaaggctta gaaggagtca cgacgaataa taaaaaggta gtattacctt aggcattgag
     1681 taaggaattt atttaggagg aaaattagat ggcgcgacca gttttagagg cgatggatat
     1741 ggatactttg acaaaaggaa ggtatgtaca gctgttagct cgtggttatt cacctaaaga
     1801 tgtattcaaa tccctgtcta aaggaacaga tatggaaaaa gaaagattac gaaaagaatt
     1861 cgattattgg agaacccata atggaatcaa ggatttgaaa ccagcacggc cagtaattaa
     1921 acgaaagaaa aagataaccc aagactgaag tgtagagcgt atgtgatcat aggctttttt
     1981 atttttgcaa aaaacaagaa aggtaggaaa actcatgaat tatgaagaaa tcgaaaacag
     2041 aaaaaaagtg tcgaaagaaa tggaagaaaa gttgctaaaa atgatgaaac aaaaacacct
     2101 taaaagatta tctgtcatgc aatatatcaa tgatatgaag ataacaggaa aagagaaagc
     2161 ttgtttgtta ggttcaatga aaaactttga acagttgagg cgaacttatg taaaaacagg
     2221 ttccaattgc caactattat tagaagtttc ttgacgatta tgatatgaaa atgatcaatt
     2281 atgatgtggc attgttattg aataaaaaga atagtaaaat ttaacatggt ttatcttggt
     2341 tagatgtagg taaattaatt catatataga aggtagatag atgaataaaa acaataaaac
     2401 tttatttaat ccttacgtgg gcgtaatcat tgtttttctt ctttttagtt attttgctta
     2461 tcaaggttat caaaaggaca aaatcgctga aaaagtagat ggtgtaacaa aaacaatcca
     2521 tattcaggaa caggctaaaa cctatcaaga taatttaacc agtttaacgg atatttctaa
     2581 gagacaactg attactaatc aaaaacaggc attagaaaag gatctcaaag gtgaaattgc
     2641 tggaaaactt gttttttctt ctacaggatt tattctccct gtttttcaag gagcgaatag
     2701 cgacacgctt agtttaggag tagcttctac ttattatcta gatactgaaa tgggaaatgg
     2761 aaattatatt cttgctggtt ataacgtaga tacaccaagc gttttattat ccgatatgac
     2821 tgatttatct gaaggagaag aaattgatct aatcgatgct cattacaagt atcagtatga
     2881 agtctccaaa agaggaaaaa tttctgatta cagccaggta gttcaatcta aggaaaacaa
     2941 ttccttttta tccttgccaa aagcaggtaa aaaaccacta ctcacactgt tagcctttca
     3001 agcaaataat atgaaaaagt gcgatgtgat ccagtgtgaa ttagtcaatg tagaaaaaat
     3061 caataaataa gggaagtgag tcacattcaa gaacttgtag aaaaattgat caataaaatg
     3121 agtgccattt ttctgttact gttggtatcc attctatatt taatatggac ggtggggtta
     3181 aatcctccta cgatattatt tatttatgat aatgctgcac agttagtgac gtttttcgta
     3241 ggctgtttgt ttcttttgaa tctgtacaag gtaaagagaa acgatctatt cttcattgca
     3301 gcaggcattc ttttttacct tttcttttct ttttttagaa gctaccgagg agatactttc
     3361 tatggtgatc tgatcgttcc gacaattatt gctattattt tgattgtaaa atggacgaaa
     3421 tttactgtaa ttgatcgtag cttgtatctt gtagtattct atgtcttttt actcgtaacg
     3481 gtttatcgag tatttactga gataccagta ccagaaggac aaagtatttg gaatccatcg
     3541 aacaaattat cagatatttg gatcaataca aatacgatag gtagttcttt aatgacatta
     3601 gcccttctta tcacaggatt tgctagttca tttggaaaat ggtacatgcg attgctatca
     3661 ttaccagcgt tgattcttgc tggggcaacg atttgggttt gccaatcgaa agcagcatta
     3721 tatgcgcttg ttatcttcat tatcttagaa attttaccta agaaggtttt tcgaaataca
     3781 cacttgtcct tttggatgta tgcagcagtt tcggttctag ctttaccgat ttcttatttt
     3841 gctgcaatgt cagacaaagt tcacttattt acaggacgag aagaaatttg gcgcaaattc
     3901 tatgaaacat taggcgaaaa aacaggacaa atatttgtag ggatgaaacc tttctttttc
     3961 catagaggaa atcagattct aggtaatcat aatacgtatc acgcactcgt caatcactat
     4021 ggattgattg gttttgttct atcgattatt cttttgctta gtttcttatg gttgctcgta
     4081 agaaaacgtg agctgacaaa tggacaacta acattcttgt gggctttttt agcgatcatg
     4141 tgtcaatcgt tcatggaaga aacattaacg acaatcagtt gggtgccaat tgtattctta
     4201 atgttagcaa tggctacaca ccaatataaa accaccagat aaatgaaata gaatagtaat
     4261 aagagaaaac actttttcgt tgtgaaaaag tgttttttgt tttgtctgaa aggaggcaga
     4321 tggatgaaaa aatggttatt tggtttttta ggagtcgctt taatcgtcgt ttgcagtgtg
     4381 tttggctatg ttagctacca aaaacatgaa ggagaggtgt ttaagcaaaa tatagaaaaa
     4441 aagatgcccg tagatcaaat caatgcacac gcaaagagtt ataaggagga tgcaacgaac
     4501 gtcaataatg atatgtcttt ggggcaaatg ttgtccatac aaaaagaagc aatcgaaatg
     4561 ggcgtcaata aacaagtatt tgcacaaatt cagattcctg cacttggttt agcattgcca
     4621 atttttaaag gggcaaatca atacactttg agtcttggag cggctactta tttttatgaa
     4681 gatgccgaaa tgggtaaagg aaactatgtc ctagcaggtc ataacatgga aatgccaggt
     4741 gttttatttt ctgatattca aaagttgtca ttaggggagg tgatggactt agttagtaac
     4801 gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgagta ctttaaatta
     4861 atcgatggcg ttcctgaaga gaactctttc ttgtctttac caaagaaagg agagaaaccc
     4921 ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata cgtggtacag
     4981 ggagagctac aataacaaga atttaatcaa cgcgagtcgt tgaaatatga aatgaggaga
     5041 gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgattgttg gaatgttagg
     5101 aatcattgca ggctgctttc aggggtatcg agcttatgca gaagatgtca cgcaaaaaac
     5161 gccaccagaa agggtcaata tcacggttca taaattaatg tacgatcaaa gtacgcaatt
     5221 gaacgttgat attgacggaa tcaaaaatga tggttatacg cacgatcaat atcctacagg
     5281 tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag gaaatattac
     5341 ggatcaagta ttaccgactg aagattctga tctaaccaac gcgaaagtgg acgaaatcgt
     5401 taaagatgta gaagacaagg gatctaacag tgagtacgtt aagaatgcaa ccaataaaat
     5461 tacttcagct gtagatgaaa atggtgagat cacttttgct gatcaaccag cctatgtcaa
     5521 ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat tagtcacaca
     5581 aaaagcaaaa ccaatggttg tgattgcgcc aatgacagac aacacaggat caggattttt
     5641 aaaagatatt catttgtatc caaagaacat tgtttctaag ttgagttttg aattaacgaa
     5701 atttggtgac gatggcacag ctcaaagcaa acaaacacct ttaaaagggg caaaatttga
     5761 gttatacaag ggcgaacctg gaaaaggaac aaaattagga gagcttgtat cggatgatca
     5821 aggaaagctg actgcaactg atttgacgtt aggaaaatac tactttgtgg aagttccttc
     5881 agaagttgta gttggatcag atcaagaacc aacagcagac caatatttgt taggcgcaga
     5941 cgctcgcaac gatgcgcaca ataagttaac ctttgaaatt acgaatgacg gcgtgacaag
     6001 tgatttaaaa gcttcctacg tgaactataa agcaccagtg atcgataaaa cagtgacaaa
     6061 tggaacagga gaagaacatt ctttccaaat tggtgacgca gtgaattatc aaggtacaat
     6121 tcatatccct acagatatcg caggtggaac agatgggatt acagtaaatg gtgtaaaaag
     6181 tgaaacttct ccttacagtg tatttaaatg gggcgacaca gcaggtaaag gactttctta
     6241 tgttgctgaa aaagcagata ttaaagtaac aaataatgat ggttcagtcg tattaaaaga
     6301 aaatactgac tataaaatcg aaaattctga tagtggtttt gtgatcgatt ttatcgtaaa
     6361 taatggacaa gtaagtgata cagttgcgaa tttacatggt caagatctaa aaatgactta
     6421 taacatgttt gtgaatgata gtgctgaaat cgcaaatcca ttaacaaata gcgtggattt
     6481 cgtttataac aataatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt
     6541 tgtgacttat ggcgcaaaat tcttaaaagt cgatagcggc ctatttggaa caggtatcaa
     6601 agcaacaccg cttgaaggcg cagaatttgc agctaagaat gcagaaggta agtactacgg
     6661 tggacttact gatacagata aagatggtgt caaagaagca gtttgggtag acgatgtagc
     6721 aaatgcagca atcctaaaat cagataaaga gggtcatttt gaaatcactg gtttaactga
     6781 aggagattac tcactggaag aaacaaaagc tcctgaaaac tatcaaaaat taacaaaaga
     6841 gatctctttc aaagtagaca aagattcata caaagaagaa aaccgaatca cgatcaaaaa
     6901 taatcaaaaa gcttccgtac caatgactgg tagcaacggt ttccaatctt acgtattgat
     6961 ctcttgcttg ttgcttgggg caggcgcaat ttctgcagtt gtttacttta agaagaaagc
     7021 ctagaaggat cagctaatga ttatcagaca ccctaagaaa aagcgaatca tgggtaaatg
     7081 gatcattgct ttctggttac tttcagctgt tggtgtctta cttttgatgc ctgctgaagc
     7141 gagcgtggcg aaataccaac aaaatcagca gattgccgca atcgatcgaa caggaacggc
     7201 agcagaaacg gattcttctt tggatgttgc taagattgag ttaggcgatc ctgttggtat
     7261 tcttacaatt ccaagtattt ctttaaaact accaatttat gacggaactt ccgacaaaat
     7321 tttagagaat ggtgtaggga ttacggaagg aactggcgat attacaggag gaaacggtaa
     7381 aaatccttta atcgcagggc acagtggact ttataaagat aatcttttcg atgatttgcc
     7441 ttcagttaaa aagggagaaa agttttatat caaagttgat ggtgagcagc acgcttatca
     7501 aatcgatcgg attgaggaag tgcaaaaaga cgaactacaa agaaactttg tcacttactt
     7561 agaaccaaat cccaatgaag atcgagtaac gctaatgaca tgtacgccta aggggatcaa
     7621 cacacaccgt ttcctagtgt atgggaagcg agtaaccttt acaaaatcag aattaaaaga
     7681 tgaagaaaat aagaaacaaa aattatcttg gaaatggctt ttaggaagta ctgtttttct
     7741 ttcagtcatg atcattggtt cgctatttgt ttataaaaag aaaaaataaa ggagggtaac
     7801 caacatgaga aatagaaaat ggctatttgt ttgtgcagcc gctctttttt ctttcactgc
     7861 ctacacacag ttgacgtttg cagacagaca agaaagcacg gatagcacag aacaaacaat
     7921 ccaaacgtca gatagcagca cgattcaaag cagcgatgaa accgtcgatt cgtcaacagt
     7981 tgattcttca gaagaaccta aaattgctga agacgatcaa atagtcaaac agaccattca
     8041 cgttcaaaaa attatttctt ccgaacaatt agacaaccag gggcaaaagc ttgaaaaaca
     8101 agaaggagta aatggcgcga agttttctgt ttatgatgta tcggacattt tacaaaagat
     8161 ggatgttaag gatctcacta ctgaccaaat tgaatcccaa ttaaaagatc gagtgaaaaa
     8221 actatccagt gatcaactga agctaatttc taatggagaa acaaagacaa ttgatcaaca
     8281 aacaggtgtc tttgaattct cggtagaagt acaggcaaat cagaaacaag cttattatat
     8341 tgtcaatgaa tcaagtcctg aaaacatttc taatagcgag gatattttgc tattaacacc
     8401 tgtttctgat aaaaatggtg aattcttaaa ggatgtgtgg atttatccta aatcagaagc
     8461 ttcgcaacct aaagaagaag taaaaaaaat cgtatctact ggcgtaaaga aaaacttctt
     8521 tgagaattgt tgggattttg ttactcactt gttttctaga gattaaggaa gaaataaaat
     8581 gaaactgatc aaattattat tattcaattt ttcttgttta accgcagtct ttttgtactg
     8641 cggtttatta ttttctaaac tatctatttt gccaagctac caactatacc taatgaggat
     8701 tttaaacata ccagctgctt ccatgccgct agatattatg gaaaagctca ttaggaatcc
     8761 attatttact acgatcaatt tgatcgttat tgtcggaatt tcactagtca tttataaaaa
     8821 agaggggatg aagggatgaa ttttaaacac tcgtggacaa gacggttaaa atatgttttt
     8881 cccataatga tgatgttagg tacattgttt gggttaaaaa cagccaatgt ctttgctgaa
     8941 aaagttattg tcgatccgtc aaatccaatc cagaacgtaa agaataattt tattattcca
     9001 aaagatgttc ctaatgcaaa aacaaatatt actgtaaacg gagcgtcaaa agtttacaaa
     9061 tatcatgttg atgccaacaa aggtggtttt acagacgact acgtaggatt agtggaggga
     9121 aacaaaaata tacgctatcc ttctttccaa aaagaatacg gtgaaacaaa ccttgtttta
     9181 gaaggcgtaa caaccaacac caaagtcaat attgactatg gaaaaatcgg tacgtataac
     9241 gggaaaaaag tcaatatcaa gctagttcta tctaatattc acttatactc ggatacgttg
     9301 ccgtggaatc tactagataa caactataca aaaactcatt ttcgagacga tggctataaa
     9361 aatacgaatg gtgcaatgag taaatctaaa aaaagaacag tactttggat ctcagacaat
     9421 cttttctcag gaattgttta ccattcaaca caaatgaatg tgcagctagt agccacatat
     9481 gaggacggta gccccgttca attttcagga gatacattta tcagttttaa ctctttgaac
     9541 cctgcaggag gaaaaagcac ggatcttaaa ggagaatatg cccactatga caaaatgaat
     9601 acgacagatt ggtatgtcag aaaagatacg gttctttcag aatttaaatc tttctacaac
     9661 aatctgaacg tggtaggtgg ccacccagga ggaagctcta agctgacaca agcggataat
     9721 gatttcaata atttgcatga taaattagga gatccgaagt ttggtcaagg tactgtgtcc
     9781 tttaaaatat ctgaagcaaa tccgacgttt gtgattggtt cttctaatgt tcaaacctgg
     9841 tttactttat cttctgcgac aatctttagt gttgttcctg atcaacctga aaaaacaggg
     9901 gttgataaaa atggaaataa cgtgaacgat aaaatgttgc aagtaggaga cacgattcaa
     9961 tatcgaatca aacagaaggt gaatcgatta ggagtggatt tactagcggt ttacgatcga
    10021 tttgagttaa tcgataattt accaaaagaa gtaaattacg tcgatgcaca cgttgaaagt
    10081 ggtacaaata aaaaattcga tgtatctggt gaagtgacct atgacaaaac aaaacaccag
    10141 gtaaaatatg cagcgaaagc tgatacatta aaaaatcgaa tgaagtataa cggcgaaact
    10201 tacgaattag tgatcaatgt aaaagtcaat gaattggcca atcaaaatag tgtagccaaa
    10261 aatcagggaa cgtcgatcat taataaagta gaaaaggata caaatatcat taccgtttat
    10321 ttccctaaaa ttccagtgaa agaagtacaa caaaatggaa aagatgtaaa cggacgaaat
    10381 gacggtaaga agggtacgcc gacagctcct ttaaacgcag gatctgaagt acaatatcta
    10441 gtcacacaaa aatggcacac aaaaggcgta gacgctgcgt cagatcacta caaacaattt
    10501 agtattcaag atccaattga agctcgtttg acatataaag aaggttctgc gcaagtaata
    10561 gataagagta aaggaaaaga tattacttct gaaggcacac taacctatga tagcaattca
    10621 agaacgctga agtgggaagc ttccgctgac ttcttaaata acaatctttt agatggacga
    10681 gaaattcagc tgatatttac agctaaaact ccattacaat cagaaaaaaa tattgataac
    10741 caagccgtag tagcagtaga taatgtttct aataaaacaa atgttgtgac aattggggtt
    10801 gatcctaact tgccacaagt tattgttcct aaaacaggtt ctacacattt agtaacgatt
    10861 ttagcggtca gcttactatt acttgtgtta gcaacttttg gctacgcagt tctgaagttc
    10921 aattaaacaa aaaaacaagg ctgaaaatca gccttgtttt tctatttgaa aatgggattg
    10981 gcactaataa atacgttgtc tgaagaaagt gatccgtctg cagaaacttc ataagcatgg
    11041 gataaaccaa cgatactttg attgttgtaa agcaagtttg cgttatgagc agcgctatct
    11101 ttccacataa ccatagcatt tactgctcct gtatctgaaa ggatctcgtc aacgtaaagg
    11161 tagaaactat tgatattttc tcctttagca ccgtagcagc ctaagctttc taagagatct
    11221 tggaaagtac cgccgccagg tcggtaatga ctgaagctta cttgtaattc ttttgcacga
    11281 atatctgcag cttgttgaac gatatcattg aattgcattg gggcaacgcc attttgttga
    11341 cggtagctat ttaaccaagc aagttctttt tgacgcacat agttgtttaa atcacttagt
    11401 gagatatttt tgtatttgaa agtgatcgtt cgattttggt caacattatt gattacacgt
    11461 tgtccatttt tatcaacggt aacagatgaa tcgtcatttc tacacaagaa gtagttttga
    11521 tcggtcatat ttttccaaga agtagagaaa ctatctccct cgtaaaccgt ttgtgtttca
    11581 gtagataaaa cagcgtttgt gtcaacgttt atatggttca cagtgacgtt gtattgatga
    11641 gtataggcaa aattgattcc ttgattactt gtaacgttgt ttaaagtcac agtgttgcta
    11701 gagctttctc taggatcaag aacaaaatta gtgaacatag gtgcttcaat tgtgatgctt
    11761 tgtccataaa cgacgggata actcttactg taattataga gttcttggat atttggatca
    11821 gtatccgaga ctttatttgg acgagctttc acttcaacta gaaagacttc atcatagtaa
    11881 aaagtaaatg tggtatttgt gttaatcgtt tgtgtttgtg tttcattccc atttacttga
    11941 tactgtggtg cttcaaagtt agtcaagtcg gttctagctt ttgcagtgta acttgtacca
    12001 gcttttactg agacatgttc ttcttttagc agcgttccag tttgtcgatt catatgacgg
    12061 acagttaccg catattctga aggtttaggt ttgtgttgta accctttaaa tgcagaatca
    12121 agactatttt tagcattatt tacttgggtt tgagaagctt tagaatcgtc tagtactttt
    12181 ttagcattgg ataaagcagt ctgaaaatta ttccaagatc cttctgtaaa atctccttta
    12241 gcagttgatt ttacttggtt gtacaaggct tgtaattccg ttttgttcac agtaggagct
    12301 ggttttttct ttaatccctt gtaagcagaa tctaatgtgt ttttggcact atccacttgt
    12361 ttttgagtcg cttttgagtc atttaacact tttttagcat tgtttaaagc ggtttggaat
    12421 gcgttccagg tattgtcggt atagtcacct tttttagttc ccttaacgct cgtataaaga
    12481 gcttctaaag ctgatttatt cactttgggt ttagaggtgt cagcaccata ccaaccaata
    12541 ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca acttgcagtg
    12601 tagtgatgcg aaccagaggt aacacctgga aggaaaaggc ggtagatcgg tttattattt
    12661 ttttcggcag aacgccaggc aggcccttca taagtccaac cgtaattttt ggtcaaccat
    12721 ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa ggcctttatt gtataaacga
    12781 tagacaaggt ggccaccgct agtcggtgct tcccaagcgg ggccttcata attaccccac
    12841 ttaattttca ctaggtgatc acgttctttt gtgctagagg tgtaaaaatg ttcatggtta
    12901 tttggattat aaaggcgata catgacgtta gttgaagcag cgtcagcaga tccagcaaga
    12961 gcaactgtgg cagcaaatgt tccaatagct ataacagttt ttgggtttaa taatttcatg
    13021 ttttcaactc cctattaaat gattaaatat tttgtttaca ttcataatat accacggtat
    13081 accgaggtag tcaacggtat accgtggaaa ttttgaaata aaaaagccgc cctatgaaga
    13141 gtgtaaggat ggctgtttat atgctatgaa gaaatggatt cggcgtattt ggttagtttg
    13201 gcagcattat caaatcgcat tttttcaatc gcactttttt tctttattaa atccgcaatt
    13261 gttgattctg cgactgaaga atgtttgcta atttcatatg ctgtcttgcc agtttcaaat
    13321 agtaaccatt cgattttctc tttatcagcg tacattgcaa tgctccctct tgattttttc
    13381 tttattatag ctcttttttg taataaatca caatatcaag aggaaaagtt tctatagaaa
    13441 ggtgggaaat tatgagtatc cgttccccgt gtatcaaaat gaggtttgtt gctactttcg
    13501 ttatttaata tactaaaata aaacaacgaa agaaggaata gaatgaaaaa gttaataatg
    13561 gtatttactt cgttgattgc catgattgtt attttaggtg gatgtggtag tgaaaaaaat
    13621 aagcaagata gtcaagataa tggcgtgtta aaaccaggaa cagtgtggaa agaagaagta
    13681 ggcggacttg ttcataattt aaaaattata gatgatacaa catgggaata ttccgaaagt
    13741 gtttggcatc ctgaacaagt acaaataaca gttgagaagc taaaagacta taaaggatta
    13801 gaaagatata aggtaatcga ttcaggtgga gtagatttat tcataggtaa aacagcctta
    13861 atagttccct ataacaaaga cggtttagaa acgtttaaat ttattccaac aaacgaaaaa
    13921 gaacatcgag ataaaagtga aatattaaaa gaatacagta ctaaatctgg atacaagtta
    13981 caaaagacta gcgagtgatt gctagtcttt ttatttatcc tgaaggagga atcgcagtgg
    14041 caaaagctaa aacgccattt gaaaaagccg cgtctaaaat cttagaacgc cgtggtgttt
    14101 ctgaaataca gtggaaaaaa gaagttcttg aaaaagcaca atttgaccta ttacgaggaa
    14161 aagataagga cttagaagaa tatatctatc aacgtgaacg tgaaaaactt gtattcgatg
    14221 aaattattaa acattaaaag gagcgaataa tcttatgttt gaatcaatta caatgaattt
    14281 tttaactcaa ccgaaagtag tagctgcagt tgatattttt agagcattac aaactgccgc
    14341 aactagtttg aaaaataatc tgatgatttt tgctgctgtt gcggcagtgg cttccattgc
    14401 tcttggaggt attttcttct tctttgggcg aagaggagct gaaaacggga agacacacgt
    14461 aggttatgta ttgttggggg taggagttat tttagctgcc gcttctattg tagtttcaat
    14521 gttctcaatg tttggacaaa ccccacaagg actataaaaa aaacaagtag gaggagtaaa
    14581 aagtacgtgg ctacttttta ctcctttttt tatgaaagga gaatattcat gttttttacg
    14641 atttcaggaa atgcaaaatt ggataaagag ttaataagaa aagcaacgaa gaaagtggga
    14701 aagcgtactt cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag
    14761 ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc
    14821 acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca
    14881 ctggattatc aaatttcaga aggtttttat cctacattga tagatttaat ccgccttgat
    14941 gtgtttaaag atacggatga aactatgagt gtggaagagt cttatcgaaa cgaaaaactt
    15001 ttacaagttt tagaagaaga gttagagagc gaccaaagaa aagtgaatca agtgagtgaa
    15061 gtcgaagtaa atgaagaagt accatgggaa cgaccaatct ttccaaagaa ggagtcaaaa
    15121 gaaaaagaga cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt
    15181 gaggaagaga acgatgtgat gggtcaattg gaggtagatc cctttgaaga aacaatggag
    15241 gatgaagaga cggacgaagt agaagttgat ccagttgaag ctaatgaaga gtcaacatgg
    15301 actgaagggg attttcagga atatattgaa ccacttatca gtgaagaagt agaaaaaaac
    15361 gaaacagaga tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag
    15421 catgaatgga tgaaagaaaa aaacaaatta tttttaaatg agctttattc gaaatatcaa
    15481 ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa
    15541 ggcacacttt tagaacagtt agagtctgtc caatcaacaa attggcatga gcttgcacaa
    15601 gaagatttat tagccacctt tgaagaacgt caacaagaaa cagatcaaga aattaatgag
    15661 tatgcgacgg aacagtcgtc taattgggag aaagtaaaag aggaactaaa taaagaagaa
    15721 cagcgagtga ttgaagaaga agtacaaaaa gtcaaagagc ggtacgaaaa gaaacgcgac
    15781 aatgcttatt ccttgtgtgt agaaaagatc aacaatttta ctaagacgaa tcaagaagcg
    15841 ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtatta aagaacgaaa agaagagttc
    15901 taccgcggtt tacgtacaga taagctaaag attagcaaag aattaaatga acaactgaat
    15961 aagctatatc aaacaacgac tgaagttctt atggaaaaac acgaacagat ccagcaagaa
    16021 ttacaagtac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa
    16081 gaacaagaag aaaaacaacg gcaaaaagaa aaagagcgtg aacaattaga aataagaaaa
    16141 cgggaacagg atcttgcgga acaaaaaatg gcgatagaaa aacaacggtt agagcaagaa
    16201 aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgtcaa
    16261 gcacagttga attatctagc acaacctcaa ccagtagctg ctgttcaaac gccagtgaac
    16321 aaccaagcaa atttaactcc ttcaccccag cgttccttta acggatggat ggtgggatgt
    16381 attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat
    16441 caatcttcac aggctgattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa
    16501 caagcaagaa aagagtatga acaacaatta gaagaattat tgaatcgtac caatcagtca
    16561 acaacagagt caagcatgga aaatagcaaa gagagtgatt ctagtaaggc taaagaagga
    16621 tcaagcgatt caactgtaca atcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa
    16681 taatgcgtgt tagagaaaca acgaagcgaa aagacaaacg attcaattta gaaaagaata
    16741 ggtatctttc tcatttctat cgctatactc cctcaggaga agagctacca gaaaaacatg
    16801 acaaacgcat ttcgaaaaag attttttata gtggacttgt atacctccct ttagagtatt
    16861 tagtaacaag agtaccagaa gttttcaacc gattggaaat tgttaaggga agcttacaag
    16921 gaataaatct gtttcaatta caggaaatgc cacaggtctt tcattttgat tcagctggtt
    16981 tttggaatcc atttactcct acaactagtt tgggtacacg tttagcaatt tcgacagttt
    17041 gtgtggttcc agctgtactt atgggattga aaatggacat gtggtttcgt cctctagcag
    17101 atggacaaaa aggggataat cgattaatga ccttaaaaga aatccaacag atttatccgc
    17161 aaatacctga aacaaaaact acttttccag gttatggagg tattccaatc acacattaca
    17221 aaaaatattg gtacattcaa acagatactg ttaatacgtg tattgttggt acctctcgat
    17281 cagggaaagg tcagattgaa gtgttagcca ccattgacaa tttatctcgt gcagaaaaac
    17341 aaagttcaat ggtcgttaat gatccaaaaa cagaattata tgttggtagt aaagatgaac
    17401 tagaagctag agggtatgat gtatatgcct ttaatatcac cgatccactg caatcaatga
    17461 gtgataatcc attggcactc atcgtgaaat actggaaacg aggagacata gatactgcaa
    17521 cacagttaac caatactttc acaaatacga tttattatga tgccaacgct tcagataaca
    17581 aatactttaa cgaaaacgcg cagaaagctg tcaatgcgct tatttttgcg ttgttagaac
    17641 aagctgatca aacaggagat tactccaaat tgacaccgaa taatctcgtt gagcttcttt
    17701 ctgagattgg cggttttaat tattatccag atccagacaa tgaattcaaa cagttgaatg
    17761 ccttagatga atttatgaat cagttgccac ctggaaacat tgcaaaaaaa caatatgggg
    17821 caacgaaaat tggatctgat aaagcgaagg gaaatatttt atcaacggca atcacgggat
    17881 tgaatccatt tactctaacg aaaattgcaa agatgacttc tcaatcaacg attgattata
    17941 aatcggttgg gtttccaaaa tacttagatt tgaaattaca tgaaagtctt ttaaatcaac
    18001 gagtcgtagt agaattttat cataagggaa agaaatttca tgaagaacgt gtcaaagtag
    18061 gctataaagg atttagtgaa atcaattttg attgtcattt agttgcaggt gatcaagttt
    18121 gtttaaaata cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt tctcaaagag
    18181 tattgaaaga cgaagagggt aatatcgttt atcggaaaaa acgaggagaa acccatttgc
    18241 cagaattaga aaaagaagcc gttctaacat tagatgagga aaaaagtaac ttattagtag
    18301 agtctattcg gatgcactat accgataaac caatggctat ttttatgctt acacctgatt
    18361 atgatccatc taaccatatc attgttagta tttttcttag tcagttatat aaagaattat
    18421 ctacgcaatg tgttaagact aaaggagata aatgtcaccg caggattcat tttattttag
    18481 atgaatttgg gaatatgcca ccaatggata atatggaagg aatcatgacg gtcacagctg
    18541 gccgaaacat gttgtgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg
    18601 gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga
    18661 cagatgataa taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag
    18721 agaacagtac catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag
    18781 atcgtatctt aactcctgaa agaatttcta cttttttaga aggagaaacg attgtactcg
    18841 ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc
    18901 atggggaaac gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg
    18961 atatcaatga tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa
    19021 ttgattatga ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg
    19081 atcaattaga agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tctcttgaga
    19141 atgagacggc taaaaaattg ttaattcaat tttacgaaga acaagcagca gatcgacttc
    19201 aagaatatgt tgaaaagttg atcaaaaata aagaaatttc tgaaatgatt gggaaacggt
    19261 tgttattgga atctgaaaag gtggtgaaag aatgatcatt ggaggaattt tttctggcat
    19321 agccgactgg tggtcaggct tattcggaga aggggatgca aaccccaaaa ccgtaacgaa
    19381 agtcttacaa gattatggga attatctgca gtatcaagat gtatttagtt ggatttggaa
    19441 cacttttctg tggggattga ttaaaggatt atataatctc aatactttac tagaaaaaac
    19501 catttatcaa agctttgatt taaaagactt attgaacgcc gctggggtaa atgcactatt
    19561 tcaaagtttg attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg
    19621 cttgaaattt tttatgagta aacacccacc aaaagtcaaa aatatcttgg ttaatttatt
    19681 tatggccatg atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag
    19741 taaaagcttt tacggagacg tcactgcagc aaataaagca gataaaacgg gatcacctgc
    19801 ttttcaaatt attcaaaata atgtgatcga tgtcagtacg ttactagcaa ctgacccgaa
    19861 taaagtagaa tccctaccaa cagataaaag aaatgcttta acagcgaaaa attttaaaac
    19921 agcgaatatc aatgggatta ttactcctga acaagctaaa ggtatggcag gtaatgataa
    19981 aagtgggtta tcagatatac aaaaggatcg gttaaaggca ttacaatacc gattggaact
    20041 agatgataat gggaaagaaa ttcctgttca aattagtgac gaaggcttag caaaatatgt
    20101 ctatacttca gggtatcgtc gatacagtag taaaccaggt attattttag ctggtgaatc
    20161 ttctttggca gtagcctatt tatttatcct atttacgatc gttacttgca ttattgagct
    20221 ggtatttaaa aaattctatt tagtgattgc cgcgtcaacg gactttgaga caggtcaacg
    20281 gatgaaaagt gcgatagaag atatttctca atccttcttg ttattggcgt ttacgatttt
    20341 agaattacga atctatacct taatgctttc aggtatcggt gatttgcatg cggcaggaaa
    20401 gatcaatgga ttcttgtatg tcgttgcttt gattgtacta accattgcgc tattcaaagg
    20461 ctctcaatca gttacgaaaa ttttcggcgt tgatacaagc ctgaaaaacg gcagtaattc
    20521 tcttcttagc atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa
    20581 ccttgcaagt gctggtaaga gtggtttaag ccatttaaat gatattcgta aaaatggatt
    20641 tggccatcga aaagatgtta cccctgataa cggggaagaa caatcagacg ataaagctac
    20701 aactgtacct aatagtaaga aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt
    20761 ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt gggttaaaag gaacagctga
    20821 agatcttaca gaaaaagcca aagacacctt agaagataaa ttagacggca cgagagctaa
    20881 agaattggct gaagctgtga agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc
    20941 ttcagaagtc aaagaaggac taaaagatgc gtatggtgaa ggacaagtca atgcagcagt
    21001 gaaagctaat acaacaaccg aaaaagaaaa tcaagaaggt atagtgattc cttcagtcaa
    21061 agatcaccaa gaagcgttgc actctaatga aaattcgatt gaagagaaaa attccataga
    21121 agaaagaaat tcagaagaag gaaaaaatgg acagttacca aaaactgatc cagaacaata
    21181 tcgctataaa cctaacagtg accttgaaaa tgagaagcga atttcagatt tatcaaaagt
    21241 aaatcaagaa gacaaagcag aaaatgaggg acagttacca aaaactgatc cagaacaata
    21301 tcggtataga tctgttaact ccgaaaatga aaaacgcatt tcagatttac caaaacgaaa
    21361 tcaagaagta caaacggatg aggattccat cgttctgcca aatgataatg gaatcgaagt
    21421 tggaactgaa aaatcaacga aaattggttt acctaaggca caaaatttac caatgaaaga
    21481 tagtcgtttg aatcaattca aggataatga tcaaagtttt gttcatcaat cacctgcagg
    21541 tactgttcaa atgaaaccaa gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta
    21601 caatttatct gaaagtggtg agacagggaa tgcgtccgtt caaaatgttg tgacaagaga
    21661 agctgcacca aaacctattc aaaacgttca tacaggttac aagccagcaa atgtggcatt
    21721 tactgcacca acaaaaataa atgtgacaag agaaacaaaa aacaggaaca atgcattgaa
    21781 agaacaacta ttgagggata tcaaacagga tatcaaacca taaaaggcct acaagttagg
    21841 ccttttttct atgtatgaaa aggagaaatc atttatggat aaatatagta acatgaaaaa
    21901 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    21961 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    22021 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    22081 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    22141 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    22201 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    22261 aaaaccagaa gagcgctcta tcttggactt atttccatat aaatcctgta cagaagaggg
    22321 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    22381 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    22441 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    22501 aatcgtgcaa aagcggcaat tattagataa aaatcgtatg gaacagtcct taggaagaaa
    22561 tctacaacga ttaaaaaaat atgaaaagaa attagtcgaa gaaatccagg aattgaaacg
    22621 agcagaacaa gaacgtcctg acttaagctt ctttttcgtc attgaaggaa agacaatcaa
    22681 ggatttacaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    22741 actctacaaa aaagaattga tcaagattct gtatcgaatg aacaatatga atgatgagta
    22801 ggaatggaga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaagg
    22861 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaagggcaat
    22921 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    22981 aacagctttt tcaccacaaa atgatcaaaa agtacttgat ccattaaagc aactaggaaa
    23041 tcgggaaata gaaattgtaa aatccaaagg gtatgattta gattttttaa aacaagtgat
    23101 gccacaaggt gggttaaatt ttgatgaaga atatgtgacg tatggtaatg gttattcacg
    23161 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    23221 gaacaatcct catacagtgg ccacactcga tgttagcaca gatgaaaagc aaaaagtgat
    23281 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcga tcagataatg aacgaaaagc
    23341 gactgaccga aataaaagcg cggaagaata cctggaacta gcggaatatg ctagaaaatt
    23401 gacttctaat ggggaaattt ctaagcaagt caaagtgaga gtacatgttt acggaaacac
    23461 gattcaagaa gtagatgaac gagcgcagga actgatcgaa aatctcaaag ggatggatta
    23521 tcgtgcggtg acttatctgc ataaaaccaa acgagattgg caatcgatgt ttaccatgtt
    23581 cgatgaacaa gctgaatggt tcggaaatat caaagggcaa tcactaccat cgttaaacat
    23641 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggaggga cacaatatgg
    23701 ggaaaccatg acaggcggcc catttgtctg gaatgcgacg tataaaaata gtgtacggtt
    23761 gtctactaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    23821 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    23881 ctttattcct ttcttaaagg aatataacgg tattatggtg cgtttagacg gatctgacgg
    23941 aatgatcaat cctttagaga tatttgccac acggacgtat gatgaagcat ccagtttata
    24001 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacacactt
    24061 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    24121 agaatttaag acctacttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    24181 tacttcctca aattcgcgtt caaatactga tacgcaagtc acagggaaag atccagaagc
    24241 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    24301 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    24361 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    24421 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    24481 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    24541 aattaaagaa aaacaaatgg aagaacaaga cttcaaacgc ttcttggcga tgattgatga
    24601 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    24661 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    24721 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    24781 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    24841 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    24901 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    24961 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    25021 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    25081 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    25141 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    25201 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    25261 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    25321 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    25381 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    25441 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    25501 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    25561 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    25621 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    25681 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    25741 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    25801 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    25861 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    25921 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    25981 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    26041 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    26101 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    26161 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    26221 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    26281 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    26341 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    26401 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    26461 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    26521 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    26581 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    26641 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    26701 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    26761 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    26821 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    26881 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    26941 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    27001 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    27061 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    27121 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    27181 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    27241 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    27301 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    27361 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    27421 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    27481 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    27541 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agtgggtaaa tcaatgacga
    27601 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    27661 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    27721 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    27781 aaagcggcgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    27841 cactgtgttc gtgaccctat ttctaatact tgcggcgggc tcgattgata attcggattc
    27901 agattcaagc agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    27961 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ccgtaggtgc
    28021 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt acgaatcagc
    28081 gtttggtaca tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    28141 tggttgtctg tttcctaaag gaactgcacg aggaattggt ggcattgaag gtggctggta
    28201 catgaagttt cccaactcaa aagctgcatt ttcctattac ggctttatgg tagccacaca
    28261 atccaatttt aatgcttgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    28321 acgaggcggt tatgccgctg caggaattac ggaagatagt ccgtattata caggttgtat
    28381 gtcaattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    28441 tgaaggtggc aataacaacg gcacgattac tggtgaatgg acgaatccat tccctggcag
    28501 cagtttagat aaaagttcct tcagcggtgg tcaattattt ggaacgaatc caggcggtga
    28561 attccgtccg aacggcttcc atgatggatt agattttggt tcagttgatc atccaggaag
    28621 tgagattcat gcggttcatg gcggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    28681 tttgggtgcg tgtgtgatcg tgattaatta cgatggcttg aatatggtgt atcaagaatt
    28741 tgcaaatagt acaggaaatt ctcgagtgaa agtaggcgat caagtgaaag ttggtcaagt
    28801 aattgggata cgagatacag cacacttgca tttaggattt actcgaatgg attggcgtca
    28861 agcacaagga catgcattca ttgatgatgg tacatggatc gatccgttac catttttgaa
    28921 tagcagtaaa aaatagatca ggagaaaaaa tatggaaaat aaaacgttaa aaatcgtctt
    28981 aacagctgca attagcgtta gtttaatcgg caatattgcg ttgttttggc agctgaaaga
    29041 ggaacaagca gcaaatacct ataaaattga agaagtggct aaaaacgcgt ctgagaagct
    29101 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    29161 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    29221 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    29281 aagcagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    29341 attaagtcct acagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    29401 aggtgacgtg caaacttacg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    29461 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    29521 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    29581 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    29641 aaaaacgaaa aagttatctt agatggctgg gaatctttat gattctcggc tttttgattc
    29701 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    29761 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    29821 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    29881 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    29941 tgattaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    30001 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    30061 cacaagatct gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag
    30121 attatgaaca acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    30181 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    30241 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    30301 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    30361 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    30421 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    30481 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    30541 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa
    30601 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    30661 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    30721 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    30781 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    30841 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    30901 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    30961 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    31021 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    31081 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtg
//
LOCUS       DAFGFZ010000020        30786 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000020 DAFGFZ010000000
VERSION     DAFGFZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.020"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(142..306)
                     /locus_tag="LR726_000762"
     CDS             complement(142..306)
                     /locus_tag="LR726_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215870.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            601..1929
                     /locus_tag="LR726_000763"
     CDS             601..1929
                     /locus_tag="LR726_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL6215871.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2131..2964
                     /locus_tag="LR726_000764"
     CDS             2131..2964
                     /locus_tag="LR726_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL6215872.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2980..3717
                     /locus_tag="LR726_000765"
     CDS             2980..3717
                     /locus_tag="LR726_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL6215873.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3872..4840)
                     /locus_tag="LR726_000766"
     CDS             complement(3872..4840)
                     /locus_tag="LR726_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL6215874.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5072..5905
                     /locus_tag="LR726_000767"
     CDS             5072..5905
                     /locus_tag="LR726_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL6215875.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5939..6778
                     /locus_tag="LR726_000768"
     CDS             5939..6778
                     /locus_tag="LR726_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL6215876.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6804..7364
                     /locus_tag="LR726_000769"
     CDS             6804..7364
                     /locus_tag="LR726_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL6215877.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7563..9284
                     /locus_tag="LR726_000770"
     CDS             7563..9284
                     /locus_tag="LR726_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL6215878.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9449..10591
                     /locus_tag="LR726_000771"
     CDS             9449..10591
                     /locus_tag="LR726_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL6215879.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10607..11818
                     /gene="thiI"
                     /locus_tag="LR726_000772"
     CDS             10607..11818
                     /gene="thiI"
                     /locus_tag="LR726_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL6215880.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12391..13038
                     /locus_tag="LR726_000773"
     CDS             12391..13038
                     /locus_tag="LR726_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL6215881.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13167..13383
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13431..16076
                     /locus_tag="LR726_000774"
     CDS             13431..16076
                     /locus_tag="LR726_000774"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL6215882.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16386..17699
                     /locus_tag="LR726_000775"
     CDS             16386..17699
                     /locus_tag="LR726_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL6215883.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17689..18342
                     /locus_tag="LR726_000776"
     CDS             17689..18342
                     /locus_tag="LR726_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL6215884.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18397..19089
                     /gene="radC"
                     /locus_tag="LR726_000777"
     CDS             18397..19089
                     /gene="radC"
                     /locus_tag="LR726_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL6215885.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19239..19439
                     /locus_tag="LR726_000778"
     CDS             19239..19439
                     /locus_tag="LR726_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL6215886.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19693..21372)
                     /locus_tag="LR726_000779"
     CDS             complement(19693..21372)
                     /locus_tag="LR726_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL6215887.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21374..21586)
                     /locus_tag="LR726_000780"
     CDS             complement(21374..21586)
                     /locus_tag="LR726_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL6215888.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21979..23709
                     /gene="efrA"
                     /locus_tag="LR726_000781"
     CDS             21979..23709
                     /gene="efrA"
                     /locus_tag="LR726_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL6215889.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23706..25466
                     /gene="efrB"
                     /locus_tag="LR726_000782"
     CDS             23706..25466
                     /gene="efrB"
                     /locus_tag="LR726_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL6215890.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25561..25758
                     /locus_tag="LR726_000783"
     CDS             25561..25758
                     /locus_tag="LR726_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215891.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25911..26396
                     /locus_tag="LR726_000784"
     CDS             25911..26396
                     /locus_tag="LR726_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL6215892.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26500..27093)
                     /gene="clpP"
                     /locus_tag="LR726_000785"
     CDS             complement(26500..27093)
                     /gene="clpP"
                     /locus_tag="LR726_000785"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL6215893.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27273..27800
                     /locus_tag="LR726_000786"
     CDS             27273..27800
                     /locus_tag="LR726_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215894.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27891..28628
                     /locus_tag="LR726_000787"
     CDS             27891..28628
                     /locus_tag="LR726_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL6215895.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28632..29549
                     /gene="miaA"
                     /locus_tag="LR726_000788"
     CDS             28632..29549
                     /gene="miaA"
                     /locus_tag="LR726_000788"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL6215896.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29551..30780
                     /gene="hflX"
                     /locus_tag="LR726_000789"
     CDS             29551..30780
                     /gene="hflX"
                     /locus_tag="LR726_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL6215897.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10170 a         5265 c         6571 g         8780 t
ORIGIN      
        1 ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt ctttttcttt
       61 tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag
      121 agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata cgaaataaat
      181 cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga cattcccaaa
      241 gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag caatcaggtt
      301 tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa tgagtatttt
      361 gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac aggccatttt
      421 cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt atgacatttt
      481 tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata gaagagattg
      541 tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa ggatgattgg
      601 atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca agcaaatatc
      661 aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat
      721 gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac ggggaaagtg
      781 gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac attccgtcat
      841 aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta cacttttttc
      901 tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac aggaaaagag
      961 gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga tggcggccaa
     1021 tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg
     1081 gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa attactgcgt
     1141 aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga cgctattgaa
     1201 gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc attaggaaca
     1261 cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca tcttgaccga
     1321 ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga tgattatgtt
     1381 agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac agtagagatg
     1441 ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat ggaaactttc
     1501 aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta
     1561 ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc aatggatgat
     1621 ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg agaaagtctg
     1681 atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc aacaaaatgg
     1741 aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat
     1801 gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt gacgaaagag
     1861 caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc aatgggtgct
     1921 ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga gctggcttaa
     1981 gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg tgctataatt
     2041 ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg atgacgaagt
     2101 agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt atctgatctg
     2161 aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac ctatcgtata
     2221 aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa aggatttttt
     2281 gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc ggatacgtat
     2341 atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga gccgggaaaa
     2401 ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac agcgccacaa
     2461 tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc ttttgaagaa
     2521 gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt ggcagaagaa
     2581 acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg
     2641 aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct gtggagtggt
     2701 aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt ttcttatggg
     2761 aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc ggaattttta
     2821 gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact gccgatttac
     2881 cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg ttcccaagta
     2941 gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa
     3001 aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg
     3061 ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag aggcagtgaa
     3121 ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga gattaatcgt
     3181 ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta caataagttt
     3241 ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt caaaatagaa
     3301 tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc
     3361 attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag
     3421 attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga tagtaatcag
     3481 catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata tgactttttg
     3541 actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga gtacgggaat
     3601 gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa
     3661 atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa taaataaaaa
     3721 agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg aaaattgttt
     3781 ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt cacagcctct
     3841 ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa atcaattctt
     3901 cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt tgactattaa
     3961 tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct tcataatcat
     4021 aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg attttttgga
     4081 caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca cctaaagctt
     4141 cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc ccttgcatcc
     4201 cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc agtcgctttg
     4261 tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg atgggaccca
     4321 atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg tagcgtgcag
     4381 aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct tcacaacttg
     4441 cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca ttacttgaac
     4501 gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca agaaaaaaag
     4561 cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata cctgatgcat
     4621 aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg tgtggtgagg
     4681 gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg atttctgcat
     4741 ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt tctttcatgg
     4801 cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact tccttacgaa
     4861 taattttgca tagccgttaa atcttattgt atactatata aaagagtttt gctatcagtg
     4921 aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa tggaaaagac
     4981 tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac aagaaagcag
     5041 ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa catcgtttaa
     5101 agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg cagggtttct
     5161 acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct cagttgagaa
     5221 ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc aaggaatcag
     5281 cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg cgcatttttt
     5341 agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag tccaattccc
     5401 ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt
     5461 tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg atgaatttct
     5521 tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca tcgatgagcc
     5581 acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga actttgctct
     5641 ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag agtggcgtaa
     5701 aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac atgaagtcaa
     5761 agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt ctggtggtga
     5821 tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag atggactcaa
     5881 tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg cgaagtctat
     5941 gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt caaaagcgtt
     6001 ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc
     6061 aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat tagatatgta
     6121 tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg accacccttt
     6181 tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc gacaaatcgt
     6241 ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac aatcgatttt
     6301 tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat
     6361 cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag agatattaaa
     6421 agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa agttagaaca
     6481 tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata tcatcccaaa
     6541 aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga
     6601 agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag gtgtaggaat
     6661 tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact ttgtgactca
     6721 atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga tttattagtc
     6781 aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa gtagaaacat
     6841 tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca gaaaatgaag
     6901 ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca gctacttcac
     6961 ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta gataaagcag
     7021 aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa caagtagatg
     7081 cactgacaga tatcctttac tttacttatg gttctttttc attactagga atagtgcctg
     7141 atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca gatggaaaac
     7201 ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag atggaatatg
     7261 ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg attgctaaga
     7321 agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag tttcactatt
     7381 tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt gaatgcatga
     7441 aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat tatttggatt
     7501 tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg gaggaaccat
     7561 aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc gccattgtcg
     7621 tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat gccttagaaa
     7681 aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat gttaaaaaaa
     7741 tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca
     7801 tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg
     7861 aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct acaatggacc
     7921 aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag
     7981 aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag
     8041 tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa cagatcaaag
     8101 tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac cctgttgaag
     8161 cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct ctgatgaaaa
     8221 aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt gaagaaatca
     8281 gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa gatcttgctg
     8341 aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt gaaaaaacag
     8401 aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat tcgttatatg
     8461 atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa tcaacgattg
     8521 cagagtatat caaacataca acaaagaata accgtcaatt attgattgag ctggatcaca
     8581 catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt ttccaaaccg
     8641 aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt caacacgaaa
     8701 ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta gaagatatcg
     8761 aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc gaaaaagaag
     8821 cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa
     8881 aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt gctactgacc
     8941 gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa gaggtcaatc
     9001 gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca catgatttag
     9061 tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat cgtcatacac
     9121 acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa gaatatcact
     9181 atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct ggtgctttca
     9241 aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa aaagttagta
     9301 aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta tatttgaaaa
     9361 ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg ttataataga
     9421 aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg caacaacccc
     9481 aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta ttgggaatcc
     9541 atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag caagaaagca
     9601 gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag gcggaacaga
     9661 aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg ggaatcatat
     9721 gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat taaaagaatt
     9781 aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt
     9841 gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga ataatgaagt
     9901 aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc ctaaaattca
     9961 tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat ggttgacaga
    10021 aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg gaactggttt
    10081 catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag ggcaagaaag
    10141 aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta aagctttacg
    10201 tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa aagtagctct
    10261 aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa ctgactatgc
    10321 accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg tccatgcctt
    10381 agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta aacatatggc
    10441 aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg ctgttcgaat
    10501 cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta ttttcaagca
    10561 gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga aatacagtga
    10621 aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa gctttatcat
    10681 gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca aaatccatgc
    10741 tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag ttattccaaa
    10801 actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag aaaaagatgt
    10861 cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg ggaaagaaac
    10921 atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta atgaattgaa
    10981 tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc aaatgcgtca
    11041 accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat cgtatgaaac
    11101 gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc
    11161 tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag tggaaattga
    11221 agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga
    11281 tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag tgccatttac
    11341 cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga caattactcg
    11401 tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt
    11461 gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg tggctatcaa
    11521 tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac ttgaaatcat
    11581 tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg aagattgctg
    11641 tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg tccatcaata
    11701 tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct tgaagatgga
    11761 agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat tgctataaga
    11821 ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa aatagctcca
    11881 tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc atttatcaag
    11941 aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta tttagagatc
    12001 aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc gaggttgtga
    12061 cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt tcaaattttt
    12121 gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta tcagaggttg
    12181 cgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt
    12241 ttacgtttgg ttgagcttat taccgcagat gcaagctttt gaacaccgtt ttatttgtct
    12301 cacacccttt tccttcctac ttgtaacctt gctcccgtgt tagaaacatg ttacactaaa
    12361 tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa aaacagatac actgccaaaa
    12421 gcaactgcga aaagattacc tttatattta cgctacttga agatgctgga tgactcaggt
    12481 ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc aaattccttc tgcaacgatc
    12541 cgcagagact tctcgcaatt aggcgagcta ggcagaagcg gatatggcta tgatgtccct
    12601 tttcttatcg atgtattcaa taatatcctt aatactaaag aagaaaaacg aatcgcatta
    12661 gtaggatatg gaaatcttgg caaagcattg aaacacaaca attttagaag aaatgaaaat
    12721 ctaaatatcg tgtgcgtctt tgataatgat ccagcactga tcaatcgagt aattgatgga
    12781 gaaatgatct atccgattga tcgtttcgcg gagatagcca aagcaaaaaa tgtgacagta
    12841 gcaatctcta cggtaccaag taaatattca caatctgcta ttgatgaaat tgtcaaagga
    12901 aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa ctgtcccagc ttatgtcaat
    12961 gttcaataca ttgatttgac aaccgagttg cagacgctta tttactttga tgagaattac
    13021 tcagaagttt tttcttagga aaatttgagt aggcttattg atcttgcgag aactcgtcgt
    13081 aattctttca aaaatttttt agtactaaat gaagtggttt tttcacgacc acttgttgac
    13141 taaaaaaaaa aaccattgta gaatgaagca taacgctgaa caagaggagt aactggcaca
    13201 ttcattctta gagagagaac tatctgctga aaggttctca atgacaacca gtgaaggtag
    13261 cttgggagtt tctgtcttga atggagtaga gatagacgag tcgtgatcgt taactcactt
    13321 gagaggcaat aatgattgat tattgcaatt taggtggtac cgcgtaaatc atacgtccta
    13381 gaatacgaaa gtattttagg acgtttttta gtttaaagga ggaaaacagc atgacggaag
    13441 aaaataacct atcaacaaaa tataatccgc aagaagttga agctggacgt tatcagcatt
    13501 ggttagaaca agatttgttt aaaccgagcg gagataaaaa agcaaaacct tattcaatcg
    13561 taatcccgcc cccaaatgtt actggaaaac ttcatttagg gcatgcttgg gatacgacct
    13621 tgcaagatat gctgattcgt caaaagagaa tgcaaggata tgatacttta tggctgcctg
    13681 gtatggacca tgcaggaatc gcgacacaag caaaagtaga agcgaagctt agagagcaag
    13741 ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga tcaagtgtgg gaatggaaag
    13801 aagagtatgc gagccacatc cgcgaacagt gggctaaaat gggcttatct ttagattaca
    13861 gtagggaacg ttttacatta gatgatggat tatctgaagc agtacgtaaa gtttttgtta
    13921 ccttatacga aaaaggtttg atttatcgtg gtgaatatat catcaactgg gatcctcaag
    13981 cacgaacagc tctatctgat atcgaagtta ttcataaaga tatcgaaggc gccttttatc
    14041 atatgagcta tgaactagca gatggatcag gcgtagtaga aattgcgaca acccgtccag
    14101 aaacgatgtt gggagatacg gcgattgctg ttcatccaga agacgagcgt tatcaagcgc
    14161 tcatcggcaa aaaagtgatt ctacctttag tcaataaaca aatcccaatc attgcagatg
    14221 aatatgtaga tatggaattc gggacaggtg tagtaaaaat cactccagca catgatccaa
    14281 acgactttga ggtaggtaat cgtcatgatc ttccacgcgt aaatgttatg gatgatgatg
    14341 gtacgatgaa tgaactagca ggaaaatatg ctggaatgga acgttttgct gctcgtaaag
    14401 caatcgtgca agatctgaaa gaaatcggtc gattgattaa aatcgatacg atgatccaca
    14461 gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc tcggctgtct actcaatggt
    14521 ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa ccaagaaaca gatcaagcag
    14581 tggaattctt ccctccaaga ttcaatcaaa cgtttttacg ttggatggaa aatgtccatg
    14641 attgggtaat ctcacgacaa ttatggtggg gacatcaaat tcctgcatgg tatcacaaag
    14701 aaacaggtga aatgtatgta gggatggaag caccagcgga tagtgaaaac tgggtacaag
    14761 atcctgatgt tttagacact tggtttagtt cagctttgtg gcctttctca acaatgggct
    14821 ggccagatac ggaaagcgaa gattataagc gatacttccc tactagcact ttagtaaccg
    14881 gttatgacat cattttcttc tgggtaagca gaatgatttt ccaaagcttg gaatttactg
    14941 gcgaacgtcc atttgagaat gtcttgattc atggattgat tcgtgacgaa caagggcgga
    15001 agatgagtaa atcattaggt aatggaatcg atccgatgga agtaatcgaa aagtatggag
    15061 cagatgctct ccgctggttc ttatcgaatg gttctgcacc aggacaagat gtccgcttca
    15121 gctatgaaaa aatggatgct tcttggaact ttatcaacaa aatctggaat gcttcacgtt
    15181 tcgttatcat gaatatcgaa ggaatgaccg tagaagagat cgatttatct ggagaaaaat
    15241 cagtggctga ccggtggatc cttactcgtt tgaatgaaac gatcgaacga gtaactgagc
    15301 tatttgatcg ttttgaattt ggtgaagcag gtcgacaact gtataacttt atctgggatg
    15361 atttttgtga ctggtacatc gaaatgagta aagaagtact ttatggagat gatgctgttt
    15421 caaaacaaac cacccgtagc atcttagtcc atacacttga tcagatccta cgtttattgc
    15481 atccaatcat gccattcgtt acagaagaaa tatgggaaca tattcctcat cagggaaatt
    15541 ctctagtagt ggcagaatat cctgttgtac gtccagagtt tgatgatgaa acagcttcta
    15601 aagggatgga agtattgaaa gaattgattc gagccgttcg aaatattcgt tcagaagtga
    15661 atacaccatt gtctaaacca attacgttgc taattaaaac aaacgatgct aaaatcgatc
    15721 aatttttagt tgaaaataca agctatatcg aacgcttctg taatcccgaa gagttgacga
    15781 tatcaagtga gatcactgcg ccagatctag cgatgtcagc tgttctgaca ggtgcagaaa
    15841 tcttcttgcc gttagcagga ttgatcaata ttgaagaaga aatcaaacga ttagaaaaag
    15901 aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg aaaactttct aatgaacgtt
    15961 ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg tgcaaaagaa aaagattatt
    16021 tagagaagca aacggctgtc aaagaacgaa tcgaaagttt gcgtactatt caataagaat
    16081 agaaaaagag agtgtgaaat cagctagcta ttactaaaag ctagctgatt gaacatcgtt
    16141 acaggaaaga gtttgagcct cactcttagc ttcgattcat aaggagatgt gacataaatt
    16201 ttgtcgcatc tcttttttag tctactctta ttatgtagga gaaggaggga ggtggatcat
    16261 gtatcaagag tcaataaagt agttgataaa atcgttcctc tctctgtttc ttttctcttt
    16321 gtcatacttt tatttttctt atgtgttcta tttgttataa taaaagagaa atgggggaat
    16381 tgttgatgac gattgaagaa gcaatagaat ggatccatag tcgattacca tttggttcac
    16441 gtcctggcct tgaccgagtc gaagctttat tagaagtggt agggcatcca gagagaaaag
    16501 taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc tactgtagct tatttgagaa
    16561 gcctattaga agaggctggg ctgacagtag gtacatttac ttcaccttat atcgaaacgt
    16621 tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga caatgatttg atccagcttg
    16681 taaaaaaata tcagccactg atcaaacaac ttgatcaatt tgaagcagta gctggtatta
    16741 ctgaattcga gacattaaca gcaatggcat ttgattattt tttgatgaag aaagtggacg
    16801 tagcgattat cgaagttggc ttaggcggct tattggattc taccaatgta gctgtaccaa
    16861 tgctgacggg aataacaacg attggacttg atcataccga aatacttgga gatacaattg
    16921 aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg cgttccagta gtaacaggaa
    16981 atattgcccc agaagctttt actgtcatcc aacaaacagc taaagcaaaa aatgcaccgc
    17041 tttatgtata cggggaaaat tataaaataa actatctgca tcctgatgag atgtggggag
    17101 aagtatttga attttttaat gaaataggaa agatccctca attaaccact ccgcttttag
    17161 ggcaacatca agctgaaaat gcaggagtag ccatcgaatt gtattttgtt tactgtcaat
    17221 tggaaaagct gcctttccga gaaaaagatg ttttgaaggg attgaagaaa tctcgatggc
    17281 caggaagaat ggagcgtttg agtaacgagc cattagttgt tttagatggt gcccataata
    17341 cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga attcaaagga tatcaaatca
    17401 acattttgtt ttccgcctta cgaactaaaa atgttagaga aatgctagaa cgattgcagc
    17461 aagtaccaaa tgtccatatt tatttgacga cctttgaaca tccaaaagcc atcgaattaa
    17521 gtgaagtatc agatttagca agtgagagag tatcaatcgt ttcattgtgg caattcggat
    17581 tagcagatat attggaaaag atgacaaatg aagacgtatt agttgtgacc ggttcacttt
    17641 atttcatttc agaagttcgc catttattaa tacaattagg aggagatcat gaagtataat
    17701 gcagtgattt ttgacatgga tggtttgctg ttcgacacag agttagtgta ttacgaagcc
    17761 acacaagtag tagcagacaa gatgggtttt ccttatgata aagacctgta tttaaaattc
    17821 ttaggtgttt cagatgaaga agtatgggcg aattatcata tgattttctc ggaatttggc
    17881 aaaacaagtg tacaaagatt catagatgat tcataccagg aaacgcttga acgtttttcg
    17941 caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc tctctttttt agaagaacat
    18001 cagattccca aagttgtcgc ttccagtaac caacgagcag tcattgaact tttattgaag
    18061 aagaacaatc tgtttgaacg atttgagacg attgtttctg cagaaaatgt tcaacgagca
    18121 aaaccagacc cagaaatttt cttgttagct aatcagaagc taggtacagc gaagaaagaa
    18181 acattgattt tagaagattc acaaaatggt attttagcag cttctgcggc tgagatcccg
    18241 gtagtcatgg taccagatct gcttccacca tcccaagaat tgatcaaaaa aacagctggg
    18301 gttgtttctt ctttacatga aatccctgca ttgttaaaat aaaaatccat ctcttttcgt
    18361 atcttatttt agataagata gaaaggagat gtttttttgg gaaaacgatt gatcaaagaa
    18421 gtacctacta gctctttacc aagggaacgt atggaaattt atggcgcgga agctttatcg
    18481 gatcaggagc ttttggcaat cttactgcgt accggtcaac acccttatag tgtgatgtcg
    18541 attgctggaa atctgttaaa aacgtttgga ggactggcct ctttacgaca ggctactttg
    18601 catgagttag aagagattcg agggatagga cgcgtgaaag caatcgaaat caaagccttg
    18661 atcgaattag gtcgacgaat tcagacggat catgaaagag cggctccacc ggttcgtacc
    18721 agctacgaat tggcccagca attgattctg gaaatgaaag atcacaaaca agaacatttg
    18781 gtttgtattt atttggacac gaagaatcaa gtgatcttaa aaaaaacagt gttcatcggt
    18841 tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc attatgctgt acgttattgt
    18901 gcagcaagga tcgttttagc gcataatcat ccttctggta atgttattcc atcgcagcag
    18961 gatatgaatt ttaccaaaag gatacaaaag tgtggagaaa tgatggggat cactgtactg
    19021 gaccacttga tcatcggacg caaacgatat tttagtttga gagaagaagg aatgatggaa
    19081 gagaagtaag ttgctttctt gcaaaaaaaa tgaagcaagt gtacaattat attgtaattt
    19141 tgtttgggaa tagaagttga aacatctgtt gcacctctat cgtacacgag caatgttaca
    19201 atttaaatta gtgcttttgg cacgaggagg atattattat ggaaaacgga acagtaaaat
    19261 ggttcaacgc tgaaaaagga tttggattta tctcacgtga agatggaagc gacgtattcg
    19321 ttcacttctc agctatccaa ggtgacggat tcaaaacatt agaagaaggt caagcagtga
    19381 ctttcgacgt tgaagaatca gaccgcggcc cacaagctgc aaacgttgta aaagcttaat
    19441 tttatctgta aatcaaattg atggtagaca tcaattttta cgagagaaca aagcgagtaa
    19501 caaaaatacc tgccacaaga gacaatttct cttgtggcag gtattttttt gtatgaaaaa
    19561 gtaaataaat ttaagaggct atgaaagagc caccttgtat caaggaataa gaacaaacaa
    19621 aaatggcttc tcatgttttt catttgtttt ctcttatatt caggacaagg tgctgatttc
    19681 ataacctctt atttattctt catctggtgt cattaccatt ggcaaaatca ttggatgtct
    19741 ttctgtacgt tcgaataaga aaggctgaag cgcgccagtc atcgcttctt tcaatttata
    19801 ttctgaacag tcaggtccat tcaaacattc tcttaaagcg tggaataaga ttcgctgagc
    19861 ttcattgatc atttctcctg attctcgcat gtagacaaat ccacgagaga gaatatctgg
    19921 accagccaag atttcttttt tcttgatatc cactgtcgcg acagctaata caagaccttc
    19981 ttctgaaagc aggtgacggt cacgtaaaac gacattacca atatcaccga tcccgctacc
    20041 atcgatatac acatctccag ccacaaaatg cccagcagga cgcgcagaat cgctagttaa
    20101 cgctaaaaca tcgccatttt ccatgatgaa gcagttttct gcaggaacac ctacatcttg
    20161 tgctagagaa gcatgtactt tcaacatgcg gaattctccg tggacaggaa taaaatattt
    20221 tggcttcatc aaacgaagca ttaatttttg ttcttcttgt ccaccgtgac cggaagtatg
    20281 gatgttattg atttttccgt gaatcacttc tgcaccagct tcagacagca aattgatcaa
    20341 atgattgaca cttgttgtat taccaggaat aggtgaactt gaaaagatca ctgtatcgtc
    20401 tggctggatt tggatttgac ggtgagtacc attcgcgatg cggctcaacg cggccatcgc
    20461 ttctccttga gaacctgtac ataggatcat cgtttcattg gcaggcaagc gattgatttc
    20521 gtgtgcatct acaaatgtgc cgtctggaac tttgatatat cctaaacgtt gaccgttgac
    20581 gatggcattt tccatgcttc gtccgaaaac agcgatttta cgaccagttg ccacagcggc
    20641 atctgctgct tgctgcaaac ggaagatgtt agaagcaaag ctggcaaaaa tgatacgtcc
    20701 ttcgattttt gaaaagatct tcaaaataga atgcccaatc gttttttctg atttagtaaa
    20761 tgttggaatc tcagcatttg tactatcaga taaaaggcac aagactcctt cttccccgat
    20821 acgagccatt ttatgcaagt ttgctggttc gccaacagga gtgaaatcga atttgaagtc
    20881 tccagtcgct acgatatttc cagaaggtgt tttcaccacg atccctaatg catctgggat
    20941 actatgggtc gttctgaaaa agctgacaga agtctttcta aaacggatga tcgtatcttc
    21001 attgatctca tggagctctg catctcgaag caagccatgc tcatctaatt tgtttgtgat
    21061 caacgctaat gcgagaggac cagcatagat aggaatattt gcttgtttca gcaagtaggg
    21121 tattccgccg atgtggtctt cgtgcccgtg agtaatcacc aaggctttta ctttgtgtaa
    21181 gttttgtatg atgtagctgt agtcgggaat gacatagtca attccaagta aatcgtcttc
    21241 cgggaatttg atcccggcat caatcaaaat gatttcatct tggaattgta ccccatacgt
    21301 atttttcccg atttcaccta atccaccaat agcaaagacg cctgtctcgt tatttttgat
    21361 ggaaagtttc atattaaaac tccactaatg agaaatcagc attttctttt tcgtactcta
    21421 gatgacttcc ttctagaagt tgaacgaatt caatattgta tggggtattt tcttccacac
    21481 gttggcgagc gtctacatcg ctttttgcct caatgtaaag agattttgtt tcttcccgtt
    21541 tagggttgcg tacttttgtt tcttgataat aaactttata gatcattgtt ttgtctcctt
    21601 tatctgcgta aagcagtgat ttagctagtt gattttttta gacaacacaa aacgagggga
    21661 caagccacct ggtttttcat atgctttgac tagaatactg gattcgagtc aaagagacaa
    21721 gcctcgtttt tgcttatctg ttcggatatt ttttcaaccg taccttctgt ataaaaaata
    21781 tacaacaatt aattgttatt ctagcataaa acagcaaata agtatagaaa tcaactagat
    21841 tgaaagaaaa aatcaatgtt tatcatcccg ctataatcat aatgactttt ttatgagaga
    21901 agtgttctat taataggctc gttgtgctat catttttagt aatgaaaatg taacaaaaga
    21961 ttaaaaggag atgagaaagt gaaactaatg tggcattata ccatgcgtta taagaaattc
    22021 ttgtttttca atttcatatg cgtatttggt tttattttga ttgaattagg acttcctacg
    22081 attttagcac gtatgatcga tgtaggaatc cgcaatgatg attttgatta tgtgaagcaa
    22141 caagggatat ttatgatcgt tattactgta atcggtatta ttttgaatat cttgttaggt
    22201 tattttggtt cgaggattac gactaatatc gtagcagata ttcgagatga tttgttcgaa
    22261 aagatccaaa gttattcgca tcaggaatac gaaaatctag gcgtatcttc attgatcact
    22321 cgtacaacaa acgatgctta tcagatcatg ttgttcctgc aaaatatttt acgtatcggt
    22381 tttatggcac caatgatgtt cattgtcagt ttgtacatgg tcatgcgtac aagtgtgaca
    22441 ctcagtttgt atgtagtagg tgcattacct ttactattat tggcagtcgt agcgattgct
    22501 aaggtgtctg aaccgttatc taaaaagcaa caaaaaaact tagataaaat caacagtatc
    22561 ttgcgggaaa atctttcagg attgcgtgtg attcgtgcat tcgtcaatga aaagtttgaa
    22621 gaaaaacgtt ttgctgtagt caatgagaat tatgcttcta gttcaaaaag cttattccgt
    22681 ttgatggctg ttgcacaacc gggtttttat ttcttattta atatcgtaat ggtattgatc
    22741 atctggagcg gggcagtgca aatcgatgca ggcgagcttc gtgtaggaga tttgattgcg
    22801 tttatcgagt atatattcca tgccttgttc tcattcatgt tatttgctag tgtgttcatg
    22861 atgtatccaa gagcagctgt ttcagctgga cgtatccaag aagccctaga tgccaaacct
    22921 tcgattgtag aagatccaaa tggtatatct gatacaaaaa ctaaagggta tatcgaattt
    22981 caaaatgtaa catttgctta tccaggccat tcgcaagagc cagtgatacg aaatgtcagt
    23041 tttacagcct ctccaggaga gaccgttgct tttattggaa gtaccggtag cggaaaatct
    23101 acgttgatcc aattgatccc tcgcttttat gatgtgagtg aaggaaagat cttgatcgat
    23161 ggagtagacg tgagagatta tcggctaagc aagttgcgag ataagattgg gtttattccg
    23221 caaaaagcgc tgctatttac tggaacgatt gcagataatt tgcgatacgg gaaagaagat
    23281 gctacgcaag aagaaatgga acgtgcagct gagattgctc aagcttcaga attcatttct
    23341 cgaaaaccag atggatttaa tgaacattta tcagaaggtg gttccaattt ttcaggtgga
    23401 caaaaacaac gattggcaat agccagagcc atcatacgcc gtccggaaat ctatattttt
    23461 gatgacagct tttctgcatt agattatcaa acggatgcga agttacgtgc tcgtctgaaa
    23521 aaagaaacga cagaatctac tgtattgatc gtagcacaac gtgtgggaac gatcatgcat
    23581 gcagataaga tcatcgtatt aaatgaagga gaagtcgtcg gtatggggac gcatcgggag
    23641 ttgttggaaa aatgcccgat ttattatgat attgccgctt ctcaattatc aaaggaggaa
    23701 ttagcatgaa aaaaaccttt tcttccttaa aacgtttagc tcgatatatc agaccgtatc
    23761 gtgagacttt tcttttagtg atcctattta cagttctgac agtggctttc aacgtggctt
    23821 tgccatatgt tacaggactt cctactacag aaatcagccg taatattgcc aatggcgaat
    23881 caatcaattt ttcttatata ttcaaatgtc tcatttggat cacagttgtt ggaatagggt
    23941 attgtatttc gcaattactt tctggtgttt tgatgacaaa tgtcgttcaa agtgccatgc
    24001 atgatttgag aagagacata gaagaaaaaa tcaatcgtct tccggtttca tatttcgata
    24061 aaaaccagca aggaaatatt ttgtctcgtg tgacgaatga tgtagatgca gtcagcggtg
    24121 ctttacaaca agcttttatt ggtatcgtaa atgcgatatt gggaattacg ttggctgcag
    24181 caatgatgtt ttatatccag ccgatgatgg cgctgatctc tatgatcatg attccaacat
    24241 cgatctggat ctcaaaacga gtgatcaatg cttcgcagaa atattttcaa agcatgcaga
    24301 attctcttgg tgagctaaac ggctacgtcc aagaaaatat gacaggtttt agtgtattga
    24361 aagtatatgg acgcgaaaaa gaaacattgg aaggattcaa aaaagttaat cacagcttga
    24421 aatactttgg gtttcgtgca gcattcattt ctggcttgat gatgcctcta gttcaattaa
    24481 ctgcctatgc tacttatatt ggtatggctg ttctaggaag cttttatgtt attactgggg
    24541 cgatcgttgt cggtcaacta caagcattca tccaatatat ttggcaaatc agtcagccaa
    24601 tggggaacgt gacacaattg tcttcagttt tgcaaagtgc atcagcggca acgaaacgtg
    24661 tattcgagat tttagatgag ccagaagaaa aagaaaacaa agtagatgct tcactgccag
    24721 aaacgatttt aggagatgtg acattcgatc atgtcgattt tgcatatgat ccgaagaaac
    24781 cattaatcca agatttgaat tttgaagtga aagctggtca aaccgtagct gtggtcggtc
    24841 cgaccggtgc cggcaagacg acattgatca atctattgat gcgtttttat gacgtagaca
    24901 aaggagcaat aaggattgat ggaatcgaca ccaaatcaat gagtcgcagt gatgtgcgtt
    24961 cgctatttgg tatggtcttg caagatgcat ggctttatca aggaacgatt gcagataata
    25021 tccgcttcgg aaagcttgat gcgacagatt atgaagtagt tgatgctgcg aaaacagcaa
    25081 acgtagatca ttttatccga acgatgccag atggatatga tatggccatc aattctgagg
    25141 gagacaatgt gtctttagga caaaaacagt tgctgacgat tgcccgagca gtggtctccg
    25201 atcctaaaat cttgatttta gatgaagcaa caagttcagt agacacccgt ttagaagcgt
    25261 tgattcaaaa agcaatggat cgcgtgatgg agggacggac aagtttcgtc atcgctcacc
    25321 gtttatcgac aatccgtgat gcagatctga ttctagtgat ggatcaaggt cagatcattg
    25381 aaaaaggaac gcatgagagt ttattggcac aaggtggatt ttatgaaaaa ctgtataaca
    25441 gccaatttgc tgaggaagca gaataagtca tataagaata aaacagttta aaacttattt
    25501 tttgttctct attcttgata atggtaaagt aaagttatca agaaaagtga ggacacggag
    25561 atggaacagt ttttaaatga aaaaattact gaagccaaaa gagaagttga tcgtttaacg
    25621 cacgtctgtc agacgaagcc ttttgattca gctgattttt ttgaaaatga gtttcaaatc
    25681 gaacgcttgg cagcacaaat agaagtgtac gaattaattt taaaaaaagt aatagaagaa
    25741 aatatagatg tacaataaaa acaggtaaaa cgtcagttac cagatactgt aaatagacat
    25801 ctggcctgag caggttttac ctgttttttt tcttatgaaa taaatatatg ctatgatacc
    25861 tttattcaat aggattagtt tctacaaaaa atgaaaggag aaaaataaat atggaacggt
    25921 ttatagcatc aaatgccaca gtgattggtg atgtgacatt gtcagaagat gtgacgatct
    25981 ggtatcaagc tgttttacgc ggggacagta attggataaa aataggacaa agaacgaata
    26041 tccaagatgg tacaatcatc catgtcgatc atgatgcacc tgtagatatt gcggagaatg
    26101 tgacggtagg acatcagtgc atgctacatg gctgtacaat cgaaaaaggg gcgctgatcg
    26161 ggatggggac cacgatattg aatcatgcgg ttattggtga gaatagtttg attggtgctg
    26221 gatcattagt aacagaagga aaagttattc ctcctaatgt cttggctttt ggtcgcccag
    26281 ctaaagtgat ccgtcctttg acaaaagaag aaatacaaaa aaacaaagaa aacatccagc
    26341 attatgtcga gataggtcgg atgcatgcgc aaggaaaatt caaggagcaa agataggcag
    26401 tagcatccat aaaataaaaa aggatggtca gtttgagttg accatccttt tttatttggt
    26461 ttagataaga gagcttaaaa aagcttcctt tgaaacagat taatttaatg aagaactgtt
    26521 ttccatgact tcatcgatca atccatatgc ttttgcttgt tcagctgtca tatagttgtc
    26581 acgatctgta tctttttcaa tcacttcaag cggttgtcca gtgcgttcag ctaaaatttt
    26641 gtttaagcgt tcgcgtgttt gcaaaatatg gcgtgccgca atttcgattt cagttgcttg
    26701 cccttgagcg ccgccaagag gttgatggat catgatctca gcatttggta aagcaaatcg
    26761 tttacctttt gtaccagctg tcagcaagaa gcttcccatt gaagcagcca tacccataac
    26821 aattgtttgg acatctgctt tgacaaagtt cattgtgtcg taaatagcca tacctgctgt
    26881 aactgaacca ccaggagaat tgatataaag atagatatct ttttcagaat cttgagcgtc
    26941 taagaaaagc aattgggcga tgatcgaatt agcaagatca tctgttactt ggccgcttaa
    27001 catgatgata cggtctttca ataaacgtga gtagatatcg taagctcttt cacctcgtga
    27061 agattgttca atgactgtag gaattaaatt cataatagag ttcctccttg atttcgttta
    27121 gaaatagtgt accataattt agtttctaat acctaaaatg tagtatacat ttttggtcaa
    27181 aaaaggtcaa ataaaaaact taggcaaatt agtcgcataa acccgcttta tggtatgata
    27241 agcagagaaa caaaacaagg aggaacaaca gattgatagt tggtattact ggccttatcg
    27301 gagtgctgac aggtgctgta gttgtctata tcggcatgat actgcaaatg cgtcacttgg
    27361 aaaataaaga acagcgtaga cgtgaattgg aattgaaagt caaagaaatc gagacgctga
    27421 atcaattaaa taaaaagatc aacgagatct tgcaaaaacg gacaacgctg atggaagaat
    27481 atgtcagctt tgatgcattt gatgattgtt atatcacgat tgacgatttt gcgtatctcc
    27541 aatcattttc agcgcaaaat agtttttatc tgccaaatta tattttagac gagctattta
    27601 agaatatctc ccatcgaaaa gtgattttat ctccagatga aacaatgaag attggtggat
    27661 atgcttataa aggcggacgc gtgattttag agaattttac ggatagtctg actgaaatga
    27721 tcaatgaaaa aaaagctcag ttgaagacta tgacgcgcca gccgattggt ttcttctcga
    27781 aagatgatgt ttggagctga tattttgaag cattgccgtt gcacatgaga gcgtggtaat
    27841 gctttctttt tttcttaatg atacaataga actgaacgga gtgaagaaag atgttacaaa
    27901 tctatgccca tcgcggaagc agcggcaccc acccagaaaa tacgcttcct gcatttgcag
    27961 aagctgtacg agtaggtgca gatggtatcg aactagatgt ccatctaagc aaagatggtt
    28021 atttgatcgt tatgcacgac gaagaagtgg accggacgac taacggtaaa gggctgattc
    28081 gagaaaaaac attggaagaa ctcaaaaaat tgaatgcagg gggcaaattc agcaaagaat
    28141 ttccagcagc taaagttcct gaattgagag aagtgttagg actgctcctc caaaaaaatt
    28201 accgagggat gctaatgatt gaaattaaaa ctgatcagta tgaatatccc ggtatcgaag
    28261 aaaaattagc cgaaacatta aaaagtagag attggccttt tcaacatgca tattgcagct
    28321 ttcatttgga ttctttggaa aaaatcgcat tactggaacc ggagatacag ctggacttat
    28381 tgatgagtac ttctgataaa aagatccaac tagcagcaga aaccccttat atagaaggag
    28441 tacatccaaa aaatacttgg ttgttcgaac atccagtcca ggcagctgat tttccaaaag
    28501 caattcgtgc atggacgatt aacgatgaag aagaaatggc caaatgtcaa gaactcaaaa
    28561 tagatggaat aataactgat tttccggaga aagctcttcg gtggaaacaa tcaacgaaaa
    28621 gtaggtaaaa tatggaaaaa gtattagtga tcgtcggtcc gacagcagtc ggtaaaacag
    28681 cgctaagtgt ggaactcgca caactatttg gaggagagat catcagtggt gattcattgc
    28741 aaatctataa aaatctggat atcggtacag caaaaatcaa agaagaagag atgaataatg
    28801 ttccgcatca tttgattgat gtgatcgaac ctactcaacg ttactcagcc gttgattttc
    28861 agcattcagg tcgacaattg attaccgaga ttgcgaaccg agggcaccta cctatcatag
    28921 ctggtggtac gggtctctat atccaatccc ttctatatga ttatcagtta ggagcgacag
    28981 aagaagcgaa tcctgagatt agaaaaaagt atgaaacgtt agctgaaaca attggttccc
    29041 aagcattatg gaatcagttg aaagaaaaag acccggcagc tgctgagaaa atccactgga
    29101 acaatcaacg caaagtgata agagctctgg aagttttaga gacgacagga catagtatca
    29161 cggcacctaa agaagagcca aagcaattgt atgattacct catgattgga ttagatacga
    29221 agcgtagtat tctttatgaa cgaatcaatc aaagagtcga tttgatgcta gcagaaggtt
    29281 tagtagcaga agcgcgtaag gtgtatgagc ttggagaagt ccaggccagt caaggtattg
    29341 gatataaaga atttttccct tattttgaag ggaaagaaac attggaagaa gcaacagaac
    29401 aagtcaaact acattcgaga agatacgcga aacgtcagtt gacgtggttt cgtaaccgtt
    29461 tggatgcaca gtggtttgat cttataagtg agccaaatca aaaaagtcag attgagcaaa
    29521 caatcaaaaa atggctggag gcggaataaa atggaagaaa aagtgatcat cgtaggggta
    29581 gaaaccgaag caaaccagcg ttacttttca gagtcaatgg aagagctagt tcagctgacg
    29641 aataccgcat caggagaagt cgtattcaca attacacaaa agagaccaca agtggatcgg
    29701 caaacgatta tcggtaaagg gaaattacaa gaattagttc aacaagcgga tgcgcatgaa
    29761 gcggatttga tcatctttaa tcatgagcta actccgagac agagccaact gattaccgat
    29821 gctgttggtc ttccggtaat tgatcgcgtg cagcttatct tagatatttt tgcgatgcgt
    29881 gctcgttcga aagaagggaa gctgcaggtt gaacttgctc agttagaata cctgctgcct
    29941 cgtttagtag ggcaagggaa aacattatct cgtcttggcg gtgggatcgg aacaagaggg
    30001 ccaggtgaaa caaagttaga aacagaccgc cgacatatac ggaataaaat ttttgctgtt
    30061 aagaaagaat tgaaagaagt agaagctcat cgagagcgca atcgccaaaa aagaaaaaat
    30121 agcgagattt ttcagattgg acttattggc tacacgaatg ccgggaagtc aactatcttg
    30181 aatctattga cacaagcgga tacttattcg aaagatcaat tgtttgcaac gttagatcca
    30241 ttgactaaga gatggcgttt tgctgaagga tttgagatta ctgtaacgga taccgtcggg
    30301 ttcatccaag acctgcctac gcaactgatc gatgctttcc attctacatt agaagaaagt
    30361 caaaatatgg atcttttgct acatgtagtc gatgctagtt ctccagatcg tattttacag
    30421 gagcagactg tcttaaaatt gatggatgaa ctgaatatga aagaaatgcc gatactgact
    30481 gtttacaata aggcagacca gatcgaccca gcgatgttca cgcctacgct attcccaaat
    30541 gtattgattt ctactcagac gaaagaaggt aagatggcgt tgatcgaagc aatcaaacgt
    30601 cagctgatgg aactaatgac cccttataca ctttctgtgc caagcaacga aggacagaaa
    30661 ttaagcgagt tgagaagaca aacaatggtt ttggacgaag cgtacatgga agaaacaaac
    30721 gaatatgaag ttaaagggtt tgcaaaaacc aattcaaaat ggttaagaaa aaaccaataa
    30781 aatttt
//
LOCUS       DAFGFZ010000021        30335 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000021 DAFGFZ010000000
VERSION     DAFGFZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.021"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..220)
                     /locus_tag="LR726_000790"
     CDS             complement(<1..220)
                     /locus_tag="LR726_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL6215898.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
     gene            complement(217..510)
                     /locus_tag="LR726_000791"
     CDS             complement(217..510)
                     /locus_tag="LR726_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215899.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(547..684)
                     /locus_tag="LR726_000792"
     CDS             complement(547..684)
                     /locus_tag="LR726_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL6215900.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(686..1117)
                     /locus_tag="LR726_000793"
     CDS             complement(686..1117)
                     /locus_tag="LR726_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215901.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(1131..1625)
                     /locus_tag="LR726_000794"
     CDS             complement(1131..1625)
                     /locus_tag="LR726_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215902.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1625..2074)
                     /locus_tag="LR726_000795"
     CDS             complement(1625..2074)
                     /locus_tag="LR726_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215903.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2078..2695)
                     /locus_tag="LR726_000796"
     CDS             complement(2078..2695)
                     /locus_tag="LR726_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215904.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2695..3603)
                     /locus_tag="LR726_000797"
     CDS             complement(2695..3603)
                     /locus_tag="LR726_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL6215905.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3616..6375)
                     /locus_tag="LR726_000798"
     CDS             complement(3616..6375)
                     /locus_tag="LR726_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL6215906.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(6372..7076)
                     /locus_tag="LR726_000799"
     CDS             complement(6372..7076)
                     /locus_tag="LR726_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL6215907.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(7088..9397)
                     /locus_tag="LR726_000800"
     CDS             complement(7088..9397)
                     /locus_tag="LR726_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL6215908.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(9592..10056)
                     /locus_tag="LR726_000801"
     CDS             complement(9592..10056)
                     /locus_tag="LR726_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215909.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(10056..10682)
                     /locus_tag="LR726_000802"
     CDS             complement(10056..10682)
                     /locus_tag="LR726_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL6215910.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(10689..11054)
                     /locus_tag="LR726_000803"
     CDS             complement(10689..11054)
                     /locus_tag="LR726_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL6215911.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(11044..11382)
                     /locus_tag="LR726_000804"
     CDS             complement(11044..11382)
                     /locus_tag="LR726_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215912.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(11372..11698)
                     /locus_tag="LR726_000805"
     CDS             complement(11372..11698)
                     /locus_tag="LR726_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL6215913.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(11676..11957)
                     /locus_tag="LR726_000806"
     CDS             complement(11676..11957)
                     /locus_tag="LR726_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL6215914.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(11959..13323)
                     /locus_tag="LR726_000807"
     CDS             complement(11959..13323)
                     /locus_tag="LR726_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL6215915.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(13336..13905)
                     /locus_tag="LR726_000808"
     CDS             complement(13336..13905)
                     /locus_tag="LR726_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL6215916.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(13877..15064)
                     /locus_tag="LR726_000809"
     CDS             complement(13877..15064)
                     /locus_tag="LR726_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL6215917.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(15128..16855)
                     /locus_tag="LR726_000810"
     CDS             complement(15128..16855)
                     /locus_tag="LR726_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL6215918.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(16852..17304)
                     /locus_tag="LR726_000811"
     CDS             complement(16852..17304)
                     /locus_tag="LR726_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL6215919.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(17416..17796)
                     /locus_tag="LR726_000812"
     CDS             complement(17416..17796)
                     /locus_tag="LR726_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL6215920.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(17793..18179)
                     /locus_tag="LR726_000813"
     CDS             complement(17793..18179)
                     /locus_tag="LR726_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215921.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(18181..18459)
                     /locus_tag="LR726_000814"
     CDS             complement(18181..18459)
                     /locus_tag="LR726_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215922.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            18507..19235
                     /locus_tag="LR726_000815"
     CDS             18507..19235
                     /locus_tag="LR726_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL6215923.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(19381..19857)
                     /locus_tag="LR726_000816"
     CDS             complement(19381..19857)
                     /locus_tag="LR726_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL6215924.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(19817..20164)
                     /locus_tag="LR726_000817"
     CDS             complement(19817..20164)
                     /locus_tag="LR726_000817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215925.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(20151..20336)
                     /locus_tag="LR726_000818"
     CDS             complement(20151..20336)
                     /locus_tag="LR726_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215926.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(20337..20609)
                     /locus_tag="LR726_000819"
     CDS             complement(20337..20609)
                     /locus_tag="LR726_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL6215927.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(20606..20809)
                     /locus_tag="LR726_000820"
     CDS             complement(20606..20809)
                     /locus_tag="LR726_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215928.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            20934..21146
                     /locus_tag="LR726_000821"
     CDS             20934..21146
                     /locus_tag="LR726_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215929.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(21179..21373)
                     /locus_tag="LR726_000822"
     CDS             complement(21179..21373)
                     /locus_tag="LR726_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215930.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(21381..21569)
                     /locus_tag="LR726_000823"
     CDS             complement(21381..21569)
                     /locus_tag="LR726_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215931.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(21566..21706)
                     /locus_tag="LR726_000824"
     CDS             complement(21566..21706)
                     /locus_tag="LR726_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215932.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(22196..22561)
                     /locus_tag="LR726_000825"
     CDS             complement(22196..22561)
                     /locus_tag="LR726_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215933.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(22601..22903)
                     /locus_tag="LR726_000826"
     CDS             complement(22601..22903)
                     /locus_tag="LR726_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL6215934.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(22903..23202)
                     /locus_tag="LR726_000827"
     CDS             complement(22903..23202)
                     /locus_tag="LR726_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215935.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(23195..23359)
                     /locus_tag="LR726_000828"
     CDS             complement(23195..23359)
                     /locus_tag="LR726_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215936.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(23365..23634)
                     /locus_tag="LR726_000829"
     CDS             complement(23365..23634)
                     /locus_tag="LR726_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215937.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(23646..24395)
                     /locus_tag="LR726_000830"
     CDS             complement(23646..24395)
                     /locus_tag="LR726_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215938.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(24398..25084)
                     /locus_tag="LR726_000831"
     CDS             complement(24398..25084)
                     /locus_tag="LR726_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215939.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(25090..25761)
                     /locus_tag="LR726_000832"
     CDS             complement(25090..25761)
                     /locus_tag="LR726_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL6215940.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(25754..26095)
                     /locus_tag="LR726_000833"
     CDS             complement(25754..26095)
                     /locus_tag="LR726_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215941.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(26273..26971)
                     /locus_tag="LR726_000834"
     CDS             complement(26273..26971)
                     /locus_tag="LR726_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL6215942.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            27058..27528
                     /locus_tag="LR726_000835"
     CDS             27058..27528
                     /locus_tag="LR726_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215943.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(27571..27750)
                     /locus_tag="LR726_000836"
     CDS             complement(27571..27750)
                     /locus_tag="LR726_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215944.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(27737..28075)
                     /locus_tag="LR726_000837"
     CDS             complement(27737..28075)
                     /locus_tag="LR726_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL6215945.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(28091..28294)
                     /locus_tag="LR726_000838"
     CDS             complement(28091..28294)
                     /locus_tag="LR726_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6215946.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(28309..29085)
                     /locus_tag="LR726_000839"
     CDS             complement(28309..29085)
                     /locus_tag="LR726_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL6215947.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            29384..29704
                     /locus_tag="LR726_000840"
     CDS             29384..29704
                     /locus_tag="LR726_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6215948.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            29722..30153
                     /locus_tag="LR726_000841"
     CDS             29722..30153
                     /locus_tag="LR726_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL6215949.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         8532 a         6328 c         4657 g        10818 t
ORIGIN      
        1 tgtttcctcc ttcttcgctt tcagccgaga acattttgta ggttcgattt gatacaccca
       61 acacactccc taaaaacgcg ccaaaaccag taatgatgac aacacagata tctgtgtact
      121 gccaattgag cgctttacca actaacccca cgaaagtagc tagtgcggga ataattacca
      181 gtgcgaacca ttttagtact tcgaacgttt tattattcat tttcttctct ccctaaacaa
      241 agttttgatt tgttgcgtgt gttccaccaa tttttctgta tgtgtatcta atctttcatc
      301 gtgtttcttt agttcttcat gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt
      361 agtcagcaaa tctaaattgt ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga
      421 tgcagcaatc ggtctaatta ctaataaaat caaagaaacg atagcggtta ttgatcctgc
      481 tatcattccc cattccccta aattaatcat gtgacaactc ctttacctta aataaaaacg
      541 catcaattaa gatgcgctct tatctttatt aatgatttta tctgcttctt cgtctgtaat
      601 gcataatgga acgaaaacca ttacttgttc gttagtgaaa cagccccagt catacattag
      661 tttgatatct tcatatgaat acatgttact tcccaccttg taattgtttt ttgatttctt
      721 cgatttcctt ggtattttgt acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac
      781 tttctgactt ttctttcaat aagacatttt cttgcttgat tgctacattg tttagcattg
      841 ctttagaatt tagctgtttc aattcatcat tttctgcctt cagcgtttcg tataatgcct
      901 taatattagc caattcatct acgctatcaa cagcattccc ttttttctct tcttcagtag
      961 ctaactcaac ccactgtttt ttatcaaaat caaatcttgg tctccagtta ggaactggtg
     1021 gtttaatctc tgtacagttt tcagggatat tttcttgatt gttcaaaatg atttgctcga
     1081 atccgtaagg cttgattgat ttgtaaacta ctttcattag atttcctccc ttaaattgaa
     1141 taagtgataa caaatgaata agctgatcca taacttgaat ttcttctcca tttaatggct
     1201 ccgtctgcgc caatagatag ttgagcactg ttcaaagtag aacggtctat tgacccaacc
     1261 agttgttcaa aactaattgg tgtccgatag ccttctggaa cagttaaaat aaccgaatca
     1321 ttcccgccac tactttttcc gtttaaagcc acaaaatata tagaaactgt ttttccttca
     1381 cgataaagct ttgctgtccc tgtattaccg ttagtgacaa ccagatttac cacttcatat
     1441 ctgttatcat cagtcgtcaa aacacgttta ttattgattg ttacagaacc tgaaaaattc
     1501 ttatgtcctg caatggcttg gtctccatct gtctgcacca atgcttcttt accatcaata
     1561 gcttctacat gcgtttttaa gtatttagca actccgtctt cttttaattg aacaatatca
     1621 gccattaaac cgtccccact ttctcaaatg taatattcgc taatccatca agtttcactt
     1681 tatcggttgc tgacattaaa cctgctgtcg tagttgtagc attacctgga tttttctgtg
     1741 ctccagctgc aattccatcc aacttagttt tatctgtgga cgacatcaac ccatttgctg
     1801 tagttgtagc tacagctgta gttgtggcat ttattccagg atcaccttta tctccctttg
     1861 gtaaaacaaa attaaatcta gctgcagatg atgttcctac attcgtaaca gaagcggttg
     1921 aaccactaga aacggttcct atggtaattg ttgctgcttg gccaggatcg cctttatctc
     1981 ccttcaccgt tgttggtttg ccttctatag cattccaatg agtttgtgga taaacctgta
     2041 caccgctttg ttttattttt acgatatctg tcattttcta tacctccccg attttttcaa
     2101 aagtaaaatt tggaattctt tcgtttgtgt aattttctgc ttgatttaca gcttcttgga
     2161 atttttgatc tacatatgac tgattaacac caccagtccc actaccacct gtagaactaa
     2221 ttgttccatc ttctgcaatt gatatatttg cacctgcttt taatattttt agagattcta
     2281 attttccctt taattcttca gagaaattga agtctgtttg cttagttgca gataaaacac
     2341 cttcttcagt aacttctaag agttccccaa cttttatacc tcctaattgt tcaggagtag
     2401 caatcggcaa aatatatgtt ccaccttctc catttacgat cttttgaaac atttcagcag
     2461 taagaatacc atcgcttgtc tcgcttgcat aaggtagttc agtaattgca ttctctaatc
     2521 ctaaatcagc tttcgttaaa atgactgcac catatttacc attaaccgaa agaacttttg
     2581 attgtcctga tatcattttt tctaagccta aaactgcaga aacatgtgta attggcataa
     2641 attgacgttt aacaccagat tcatcagtct ccatcattct ctttacttta accatcaaat
     2701 caccccaact ttctcaagag tgaaaacatt ctgttttgga tcatcaacag ttgcaataat
     2761 caaagctcct tcttcaattg gaaattctac tgtttcaatt ttttcgactt cgtgattatc
     2821 tgaaaagaga tcgtcttgaa gaatatcagt cacttcaatt tcaccgtatt caatcgtgaa
     2881 aagtgctgct ttcatttttt gatacaaata atttagatcg gataggaaac gttcagaaat
     2941 cgattcatgg cgtacacctt gaagatctac acgtgcatcc attaattcgg ctaacattac
     3001 tccacctgga tcgatagact tcaaaatatc cttgattgat tcaaaccatg acaaataatc
     3061 tgattcttgt ccttctctcc aatcttggaa actattctcc tgttcttctc tccagcgatc
     3121 aaattcttct ttcctttcat tcatccagtc cgtaaaatcg cctttatttt cattaataaa
     3181 atctgtcata tcagcaatta aatcttcaat tgattgccaa taagaaccca tttctccttc
     3241 tgtttttgaa acagcattga tgacaaaata ggagaagtct tgagttgttc caatcaagtc
     3301 ttctccttta aatataatga aattggctgt ttgtcgatgc aaacactgca tggaatattt
     3361 atcaaaaata tatttgattt tccctttttt agcatccaca attcttgttt ctaattggac
     3421 tggatatttt ccgccaacaa ctgattcaaa atatacctta cattctgata aatcatatgg
     3481 aagaccattt tcgacaattg tagcttccat aacttcagtg tttttattgc cttgtcgaac
     3541 ttgaatcatc cccacgtaat tatagggttc tgttgtactt aatatgacat tccactttgc
     3601 cattaaatca cctccctatt ttggtggtat gacaatggaa gaaattgcac ttgcactata
     3661 atactggcga tctaactttc cacaaatcat acctaactct gtgttttgtt cataagtttg
     3721 catacgacca ttagctaagc ctcggataac gccagtatgt ccataagttc cgtctgcaaa
     3781 ccatgaaccc acttgtccac ctcttgccca attaataatt gcaccaacta ctaattgatc
     3841 atatctaggg ttttgaatca ctttccaacc aacagcagac caatcatagg caataccaat
     3901 atcagatgct gcagaagtat ttcctatcac atgagttaaa ccatattttg ttccagcacc
     3961 catgccacaa ccacccagat atcctgaata ttctgcagac aagccataac actgaccatt
     4021 gccaattcgt tgtccaatca aagactctaa atgttttagt cctgcttctc cagtagctcc
     4081 tcctggtttt aaatctttaa atttgttata ccagttaact gcataatctt gacgttcagg
     4141 atgtgtcgct gccggacgtt catagtttcg ttcaaaagca tatgctgctt gtcttggatc
     4201 agtacaggcc ttaaatccat caaccgtcgt aggttgtact acgcccatcc attgtccatt
     4261 tgtaaatgtc caaataagca atcgaacttg tgcatctaag ctcgttattg gttctttaat
     4321 acctgcagca ttaaataagt tttgaacata aacttttcca tcccatgttg ctggaccaac
     4381 aagaggatat gaagaaccat cccactgaac taatccgtat gctggtccac ctatttgaac
     4441 agtatcagga tcaaaagttc ctcctgtttc ttgttgaatg ttccccaata ttccacatgc
     4501 agattgtttc gtaaatccgt tattacacaa aatatcgtat attctccaag ctctcttttc
     4561 tgcatctgtt tttaattcat caggataccc accaggtgtt tctcctccgc cagatggacc
     4621 gccattttgt cctggtatca cttctttacc tctaacagta agttttccct gaatatctaa
     4681 atcaccgaaa taatatgctt ttccgtttcc taataacaca aatccttttt ctgcagttgg
     4741 agaaattaaa atgtattttc catcgccatt tgtacgtatt accaaagaat tttcctcaag
     4801 aggtgtaggt gttacagcat ttggaaatgg atttcctgct gaatctgttg ttccgatcgt
     4861 tccaatggat tcttttctat tccagaattc catacctttt ttggttagct ccatgatttt
     4921 tttgttttca ttccaaattt gaagcgtgcc ttttacaagt tttaacacat caccataagc
     4981 attaaaggaa gtttcgaata cttctgcatt tattgttcct actttgatga agtcagcaac
     5041 aatttctcct tttgatgtga tagcaatacc gaacgggcca tttactccgt tgtctgaata
     5101 acctaagcca tttaaattcc aacgccacac tcgctttgca ttcgccacat ttggagtatc
     5161 catgataaga atctctgatg gagctttttc tggacgaaaa acgacatgcc caccagaatt
     5221 accagtgatc catgccgtcg cattcaacac attttgaact aacgtttctg ttcgattgtc
     5281 gattttcttt ttcaattctt gtgcttgagt atttactgcc gaagtgtaca gtgataaatc
     5341 attacctaaa acaatatcct tatatttccc aagcgttggg aaataagtgt attcgaccat
     5401 acgctctttt atctcgatat ctagctcttt cgccctaaca tgagcgacat caccaaagtg
     5461 taaagacgct agctcttgat acatgtcacc gtattcgagt gtgtgttcca acgccaccat
     5521 actgactgta tgagtagctt ttggctcatg gatacgatca ttgtcaaata aggttttgcc
     5581 ccatttgatc aattcatcaa ctgttttaca atctccgttt tcacgtttgg caatacgtcg
     5641 attctcgttg gttactccat caatttctaa ataaccatat tcgataggtt ctttgtcttc
     5701 atcataatcg ttatcaggaa caccgccaac caaatacaaa ctattgacaa ttgattcttc
     5761 atcaatttct tcttcgattg cttctaaatt gatgccaaaa tcaatccgaa aaccattatc
     5821 tgatccaatc tgttttacca gtttcaaatc atagttatcc atatcaagtt ccgcgctagt
     5881 cacgccagtc aaattttgat taccgttatt tgaaccaatg attgcttcaa ctggcgcgac
     5941 ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa agatagttaa atttttgatc
     6001 aaatgccaaa ctgtttttaa gattggtcat gatctgagac ccattgccgt tatccgtaaa
     6061 cgacttgaca ataaagttct tattggccat aaaaccaata tgccgagctg ttacagaaac
     6121 ggaggtcaaa ttctttttga cattataaat ttcaaaatat tgccatgatc catctggtac
     6181 cttcgctttt ataaaatttc ctttttttaa ataagagcga tactgtccgc ttcttgaata
     6241 gttaccataa aaacgatatt ggccattcag cacacgattg atttctggca aatcttccca
     6301 atcaactaac gacgctccgt tcacgctcaa atcatctggc atttttttat aagcataaat
     6361 aaattcttgc gtcacagata cacactccta ttccaaaacc gcacttcttt aaattttccg
     6421 gatattttta cctgattcca ttcaggctgc aatactggcc aatcaccacg cgtaaataaa
     6481 ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa ccacgattgt tgcagctaac
     6541 gtattaagta ttgttaggct tttatcaccg acagcgatcg ttatatctcc tccgttcgat
     6601 tctatttcca gatagggatg tgcaatttcg tcaccatgat caaaaatatc cataacacta
     6661 gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga gtggctgaca acgaaaagta
     6721 acatcgaatg tataaaagaa gccccactcg ttttcgaaag ggacttcttt atccatgctg
     6781 cagatagcct ctagatactt gtctggatca ttatgtgtga ttaattggct tttaccagtg
     6841 agccatcgtt tgacttctct cagtttcgaa tgtggaatag tgatcccttc tatttccaaa
     6901 tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc cgcttcggcc ctgaatcgtg
     6961 taagtttcgt atcgcttgtt cggcataata tctggaagct ctgcctcaat gatgcaattc
     7021 atatcaatta ctgcattccg atttttccaa ataaaatttg gttcatctgg attcataaat
     7081 tgtcgactca aataggaaca cctcccaaat cacgaatagc ctgtttgttt gctttagcaa
     7141 atttacgatt catacgatct aactcagatg gattgtttgc atcgactttg ccaatgtgta
     7201 tatgctgttc aatgctacct ccagaaactc ttccactgat ccctttgcgt ttttcttcgt
     7261 ctgataatgg agtaactgtg gttttaccat tcttggcagt cagtaactca ggtccagctt
     7321 caccaacaat cgcttgtcca ttgattagat gaccgccttg tgctaaataa ggcagttttg
     7381 aaatagaaaa tgattttcca cctacaccag gaacccattt tggtatcttt atattattca
     7441 atccaccaat aaatccattg atcaatccaa tcatggcatt aataggagct ttccctacag
     7501 caacaatacc atcgaaaata cctccaaaaa tatcaactag accttgccat gcttttgacc
     7561 aattccctgt aaatactcca gtgataaaat ctagaaaacc actgaaaatt cgttttccag
     7621 cattataaaa attattgaaa tttgctataa caccatcgaa aaatccacca aaataaccac
     7681 ttaaaaaatt aaatatttca acagctacat ctgaaacacc tttgaagaaa gaattcactc
     7741 catcatgaaa ccatttaacg ttattatatg cccaaattag tccagtaact aatgcgccaa
     7801 tagctataac tgctaatgca aaccagcctc ccgacattcc aaataaacca gcaagtcctt
     7861 gccaaactcc tataaaactt ttaacttccc caacgatttt tgtaacagat cccataagtg
     7921 aaccaagaac gattagtact ggtccaactg ctgccgctat accagcaatt gttatgatcc
     7981 aattctttgt atcttgatct agagaccccc accatttaga gaattcctgt aataaggaag
     8041 ttgctttttc aaaaaaaggc aaaaggctta cttggactgc ttcacctacg tcagccattg
     8101 ctaattttgc attgttcata gcttgatcgg cttgatcaat tgggtcgaga gtagcatcga
     8161 atgtgtctcc tacagctccc ccactttctc cagcagtttt tgctaaatct tccaggttca
     8221 aagtacctct acgaattgca tcggccattc taggaccgcc tttagtcccg aatacttctg
     8281 ctgctgcatt gatggcttct gtttcagaac ctgcattttt taccttgtct tgtagttctt
     8341 ctagtccttg acttaaagat tttccgtctt tcgcataagc cactgttgct ttagataaac
     8401 tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa ttgtccaagt aaagtgaccc
     8461 cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc gccatcaatt gctttttgca
     8521 tcaagtcgtc tacagattga ccagtattct gcgatgtttt cgttgttaca tcaaggacag
     8581 aattcaaatc atcatattct agtccataag catcaatagc ttgtcgagca gatattgctg
     8641 attgcgaaac atctgtatca ttgatttcag cgtactgtag gagataattg gttgaatctt
     8701 ccagtttttt atccatgaat ccaaattgtg tatttacctc accaatagct tccccaaccg
     8761 tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa agactgtgaa agtctatcag
     8821 cttgatcacc tgttgctccg gtttttgtaa tgatggtatc aagtgcctcg tcaacttcac
     8881 caaatgctgc aagtcctgct gctcctgccg ctacaatagg cgcggttact ccaacagaca
     8941 ttttttcacc aacgcctttt actttctcac ctgtttcttc gattttctgg agtttttttg
     9001 ctgtatcaac agaaacgtca ccttgttctt taagtgcatc gttcgtctgt tctaatgctg
     9061 tacgaagttt attttctccg gtttcagaat tgagtaattg tttataaagt ttttctgatt
     9121 cagcagaata ttcaccggtt tctttgacag atttttcata ttcctctctc agcatttgag
     9181 atcgttgttc agccaatcct aattgttttt ctaatttttg tttagccgct cgcaattttt
     9241 ctgtttgagt tgcatccttg tccatcgcag acacttggtt tttgtactcg gcagcggcta
     9301 agttcatttc tttgttgatt tctttgattg ttttcgaata actgacttcg ccattcgttt
     9361 taaaattaag aacgacatca gattctttac cagccattta tcttagcgct cctttctacc
     9421 accaaggcga tttatccata gttacagatt gaggtggttc aaaatcggtg ttttgttgta
     9481 accactgtaa ataagatttg agccacaagt taggtgtaga ctttaaaaag aaactctcac
     9541 tccaattcaa tagagtgaga gcaacgtaaa tataaaaact ccaaggagtt cctatctctt
     9601 ccgattcttt ttgtttttct ttctttttgc ttgcgctttt tgaaagtctt gtggcttctt
     9661 ggattttttt aggtcttcca cctgaaatgt ctggctagca aaaatttcca tacaggtact
     9721 ataagcagac aacacctctc cactcattcc caaaaatttg aaaatagttt ctggatcttc
     9781 ctctaaacca ccagtacgca gcatggcata aatcaaggca cgcatgattt ttagatcact
     9841 aggcgataag ttagcagaag aaatttgtcc ttctttttta gatagcatgg cgttcatatc
     9901 agattcaaat tgcgaataat ctccaccata cacatctgct ataaattcca tcgtttgcat
     9961 agtaaatgaa atagggaatt ctgcgccttg aataattaca acagcagaat tttttaagtc
    10021 ttcaacgtga attccataat cagataaccg agccattaac ccgcacctcc aactttagcc
    10081 aatgttttcc actgttcttc atcgtataca ggttgagcaa tgaatttatt aaataattct
    10141 agagaagctc cgtctctatt tgaatcaaaa cttgcataca tcacattatt gtatttcagt
    10201 ccgatggaaa ccagatttgc agttacatcg tcaatttttg tttcttcctc tgcagtggca
    10261 tactcttcgt caatcacatt ggatagttgt gtattaggat accaaactgc ttttttaccg
    10321 ccattttcaa tatttccaat aaatccaaag gcgaagtacg gaaattctcg tgcggtattt
    10381 ttgctaaacg ttactccgct tttagcaact aatcctttga tttcatccat cacttctatc
    10441 ggaataccca cgtgatccaa cgcaagttca tgttttgttt ctcgcgacac acgacgaaac
    10501 attttgctgg atgcccattt ttctaatgct gttccattcc ctttgacacc gatttttgtg
    10561 gcaattggca atcgtaaaac ttcactataa gtcggtgcag ttccaacaga atctggcgtt
    10621 gccatcatcg caattaaaat gtcgtccaat ccttcaaaat aaaaagtatc ttgttttccc
    10681 aattaaaatc acccttccca taaatcaagt atttttctag tcataatttc ttcaattttt
    10741 tctttgtttt gttcatacgt accacttgca aaatgttggg cacgttgatt tgttgtgcca
    10801 ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt gaactttcac ttgatcgttt
    10861 tcaactacta cttttatttg atcgcgcata tgtttctttt ttaagagcga cttaggaata
    10921 ttaggaagca actgctttag ataaaagtta gctgcttctt ctaaagattc caacgataat
    10981 tttttcggat caacttttga aaggtttcct aaataatccg atatttcaga aaaaccattc
    11041 ttattacttg gcattttcca cacacctcac atatgtgtaa tagttggtta cggtatcgtc
    11101 gttctcatca ccttgtatac ctgtaaaatc tgaatatgga atgccagcat tttgcagcgc
    11161 ttgttcaata acaactaaat cctgttctgt tccaagtgta aaaaaagaga cttggtaata
    11221 aggtaatttt ttatagactt taccggatgc catttttttg ctgttactca catttgagta
    11281 cacaatgtat ggatacatcg tccctaatct ggctttgtct ctgaacactg ctaactttgt
    11341 tgatttcagc gctgttttca attcatcaaa gctaatcgac ataagctaaa ctcaactcca
    11401 tttctcttgc atcgggattc gtataaatgc gagtaatgtt atacgttaca gaatcaattt
    11461 tgagcgcact taatttctct gtgatggatt tatcccatct gactttaatt cgtctgacaa
    11521 cgtctgtctt ggcttgctgt gataaatatt tttcttgaga agtcacaccg agttcttcat
    11581 agaatattag acgcttaaat tcgtaaattg tagttggacg atcgtttccg tctgttcctg
    11641 ttttgatgtc tagcaattcg gctttccatc tgagattatt ggtctgtctc ttcggcattt
    11701 tgaatcgctc cttgcacgat aaatggcgtc atggcattca tagctttgtc gagttcatcc
    11761 tctgaaactc tatattcata ggcaatgccg gcaaccatca aaataagata ttcttgttgg
    11821 ccaccagttg ctgttttgac ataatctttt gccatattta aataaaaaga gagcaaagaa
    11881 tcatccatgc cctcttcaaa atgaatatgt gatttgaatt tttcctctaa agacaattct
    11941 ttagtttgct cttccatctt aaccaccaac tggttttgta atttcgtagc gatatactgc
    12001 cggttcaaat ggagaataaa ccaattgacc atctagcaag ttgtaaattt ggaatccaat
    12061 ttgattttta ccagagaatt tttcaacaag tttttgaatt tccaaggcac caataacttc
    12121 ttgaatttta aatgcagaaa aatcgccaaa atataaaact ggcgtgtctg gttcaccctt
    12181 tttatctgct gcatctgtcc aatccacagg atagccaact aattggtaac caattccacc
    12241 ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca tctgttttca ttttttcaat
    12301 ggcagtcaaa gcagctcgat taataataaa gcgtcccttt ttcatcactt ctgtcactgg
    12361 tgtattttta aattcgatta atgcatcata taatttttgc ccagcacctg aagcagttag
    12421 atctaaaggt tttttaaatg ctacagcctt tttggctaat gcaccaggat tttcatttcc
    12481 agcgtcatca ccattgaaca tataattgat ttctttacgc acataagctt ttttcaattc
    12541 ttccacaaca atatcttcaa ctggaacacc agacattttt aataattttt tagttactgt
    12601 tgccaaagca tcgaattcgg caggattaag caaaatttca tcaaactgaa tagctgtttc
    12661 agcaatatca gttgaacgct ctttcttgtt tacattcgca tctgctttct tcacaagaat
    12721 tggatatttg acatctcctg atgttcgcac cactgttccg tatttacgaa gtaaattttc
    12781 ttcttgagca taagtaataa cttcagatgc aattacttct gggacagtaa ctgaaccgtt
    12841 gccagcttca atccctaaag ctcgagcttc tgcttcagaa atatttccag ctacaaaatt
    12901 agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg gtcatttttg cacgtgcctc
    12961 caatccgttc ttaattgatc caagtaatcc atctcgttgc tgttgagtaa tcattccaga
    13021 acgattttct gaactatctt cactgtctga atcttcttct tgatcgttgt cttcaccaga
    13081 acggctttcg tctgaatccg tactattcga ttgatcatct gtattgtcgt ctgtttcatc
    13141 agttccagaa tctgcgccta attcgtcttt aattccgttc aattcatcaa taacactgtc
    13201 aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat tctcctgatt caactttttc
    13261 acgtaattca gtcaatcgtt gctcacgacg tgccttcatt tttttcaata attctttatc
    13321 catgtatttt tcctcctacg cttctagcgc ttgattaatt tttttgatta atttttttct
    13381 ttcttcgacg tttttttcta attcatcacg acttcttaat gcagcttctg tatcttcgta
    13441 tgctggcaaa ggcacaatag aaacctcgta taattccact tcatggattg ttcgaagcat
    13501 tggttcagaa ttgtagtccc acgtttcttc agtcggcaca aaaccaaaac tacattggtt
    13561 gatgtctcct cgttccatag atttaactaa gtcccttgct accgttgtgt ccggtaaatc
    13621 aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga agggttccgc ttttagtacg
    13681 tcctagcaca ttggaccaat cgtgattgaa caaacaacga acatccgaat tactaattgc
    13741 ctttgagaat gcaccaggaa caatcacttc gcttaagtca tcccataaaa gtgtttgact
    13801 gttgaataca gcagcgtagc cgctaatggt cctcgtatga gtttcttcat cggaacgtgt
    13861 tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc ttcatttcca tcacctcctt
    13921 tcaagtcttg atcattcgtt ggtaaggaat catctgttgc atttttctgg ccaatcctag
    13981 ataagtcatt tgaaatatag atagcttgtg tttctggagt attctgttta ggaaaaccaa
    14041 gcatttctgc cacgttatct ggacttgtaa tcccggtacg aacgatgttg tagccaatat
    14101 ttgtttttgt tgagtaagga acaaaatcca aaatattaat tttccattcc actctatagc
    14161 cagaattagg cataaaaaaa agagcggtgt aatgttcgct cttgtttttc aatattggtt
    14221 tgattgcttt gttgtgcaga tacatcatcg ctttttcaat atctgtcttc atcagcgatt
    14281 gatacgtatc aacattgatt cctagaaatt ttcctaagtc ttttttgtaa acacctaaat
    14341 aattcaaaat tgccgcgtca tcaacaggac tttttaatgt ctcgatggaa tatccttttc
    14401 ccaaaggaat catcttaaca gaatgatcac tctcattttg cgttccttcc agttgatcca
    14461 atatagcttt gacaattttt gtttgggcgc tattatttgg attgatgtgg gcgtctagtt
    14521 taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc cgtcaaaact ttttcagcgc
    14581 tcagaacgcc ttctagagtg tttcttgcaa gatcaattat tccagcacct tttaatgaat
    14641 cagttccgat gttcttaatg tgtcgaatca tttgaccagg tattttttga ccattcattt
    14701 caaattcttc ttgaagtcgt tcattgattt tagttgttac accgtacgcc aaatgaagct
    14761 gatccctatc cgttaatggg aatgtctcac cattgatcaa taaagtattt gtttccaatt
    14821 tggtaaattc gaatcctgtc aaataatcat tgggactctt caaaatattt agtaagtgat
    14881 gatctttgac ttcgttgccg tcgggaccaa taacaattgg tgtggccaat gccacctgat
    14941 tagatatgtc ttgaactaat tcataaacat cagaagattc catgatagat gaatccgtca
    15001 catatctttg accataacgt gtgtagtgtc caaaaatatc ctcgatatat ccacgttttt
    15061 ccataaaaga atagactgca ttcgataacc gatcacgtaa tttcaatttt tttcaccgcc
    15121 tttctattta tctatagatg gaacttaggt aatcatctaa ctcatctgaa tcaatatctg
    15181 tcatttgatt catcgtttcc ttatgaccac acaaaaacgc cacgaaccca tcaatctttt
    15241 tctttgattg acgtttactt ggcgcttttt gtccattgat gttagtaatt gctacaacgt
    15301 tcaaagtgca ataaaggaat aatggattat caaattgaat tcgtttctca taaaacaacc
    15361 gttcgacatc gtcaaaagga gcattcaata ctttaggata ttgagcaact tcaacgcatt
    15421 ccaatcctaa gttttctaat ttctcaacaa gtttgtcact catcgctgga tcataattca
    15481 cttgctgaat atcatataaa ttcatgcagt cctctatgta ctgcaaaatc tgatcttgat
    15541 caatcatttt tccgtcgcaa aattcaacga aaccttgttc agccaaatca ctgtaaggaa
    15601 cattatcttc tttttctcga aactctaaat cttcattcgg aataaaataa agttgcttta
    15661 ctttaagaac cgctttacca tcttcatccc atgttgggaa gtttaaagat acacaggtca
    15721 aatctcgtgt acgtgataaa tctaaaccga tataacatgg ttcgccactt aaatcaccaa
    15781 gttcttgtgt ggtaactaaa catggctcta cttgatcctg ttcaaagaaa ttatccgcgc
    15841 cgttcacaaa tacatccaag tgtttcgtta gaaattcagc tttggaatga gctgaccgtt
    15901 gcgctgtttt aaatgccgat tctaaagcag ataaatcgac tgatattccc cagttaggat
    15961 tgcacatttc ccagactttt ctatccgtcc aatcataatt tttatttggc tcatagatta
    16021 gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc ttttgcttcg cgatatacac
    16081 gcattccaac agacgacgaa cctttcccag cagttgagat attaaacatc aatggctgtg
    16141 gtaaagatat ctgagcagac ttaaaattgt catactgttc cattttctcc tgtttatgca
    16201 attcatcgtt caatacaaaa tatggattgg atccctcgat attatcaata ttcttggttt
    16261 gaacaataaa tttatttgag taagccatat cttcatgaat atagtcataa gtaatactag
    16321 aaacagttcc ctttggcccc ctaaatattt tagttccttc aagtaggatt ggattgttta
    16381 agattgtaac tgcaaaaggt ttggcagcat attgcgcttg agcaaaatca gatgcgcaag
    16441 catagcaatc aactgataac gccccttctc cgtacattgc atatcctaat gaaccgacag
    16501 caatcaatgt tttaccattc ttttttggaa tctggacata agcctcacga gtaacgcgta
    16561 caatttggcc tttttcattt tctttaaccc aaccataaat ccaagaataa ataaattttt
    16621 cccagggttc taaaagaaac ggttttccca ccatttctcc ttttgtgtga acaataaatg
    16681 attctaccca atccatcatt tcattcgcgc gatctacatc aaaccaaata tcttttcttt
    16741 ttttccactg ataccagcga tctattgcta aacgaacggt cttaggatac ttcttaggtt
    16801 tctttcttac ttcttttgca aataaatcag cataatttac accaggttca atcatttttc
    16861 agcacctgcc ttcttacgcc atttatttct gtgcgctgct agttcatcta ctggctttac
    16921 ttctgggcgt gtaatttctt catcttttct tgcggtcgat cctccagcaa ttagtctacc
    16981 agtttttgct ttgtttgtta atcctaataa atctagagct ttcatcttct tatctgccca
    17041 agtttctact tgttgtgcca atggatgttt tgattgatta gtagctcctg atttattcgt
    17101 gaatttttgc gtctccggaa atcctttttc cttccacaat aaatatttgt gttggtaaat
    17161 ttcaaaaata tccaagtatg attcgattaa tggatcaaga gtgatggtgt acaaatcaga
    17221 caaattcatt atttctaaga tacgagcttt ttcagcactt actttttcat caataatcgc
    17281 tttacgttgc gctttagtcg tcatttttgt atacaccccc ctttgttttt tgaaaaattt
    17341 gacctaacga tgcgcgtgac tccccgctac cctatctccc cacgcgaaaa aattttgaaa
    17401 atgaataggg gggcttcaat tgaaatacga aggaaaaact tttttgtctt ccgtttcgtt
    17461 ttcaacaatt ggatgacatt ttgaacataa tagcatgaga ttgtttggat caagcttaag
    17521 cagttcgttg tctttgattg gtacaatgtg atggacgtgt gctctctttc caaatatgaa
    17581 ctgaccacac cgctgacaat gaccaccttc tctttcataa ataaattgac gcatatcttt
    17641 ccatgcttgc gttcgataga atggtttgtt ctcatgatga taaacagact ttgcttgctg
    17701 cttcttcttg cgtgatctgc ttgatctctt gtgttcagta cagtagatac cctttgctat
    17761 tttgttcgtg catccgtcaa actgacagta tttcattcta cttcacgaat aagatttatg
    17821 atatcgcctt ttgcacggat agcaccagga atatcaatac catgtttctt agcatatgca
    17881 cgcaattctt ttgcagtcat attgtccagt tcatccgtat cttcagtgga ctgatcatta
    17941 gtagcttcgt ctccatcaaa attagcagca gtgtttccat cgctatcaag aatttcactg
    18001 ctatgagcta tgagttcgcc attgacagca acaaatgatt tgccatcact caaaattcct
    18061 ttttcttctt ttacttcaaa atttggttct tgatctttcg gaacaactac tgtccttttc
    18121 ttttctgaat cccaatactc tgttccacta atggatgttc taattttgat cattgccatt
    18181 ttaattctct cctttgtaat ttgtacggat tacttttgcg cccattcgct cataccattc
    18241 aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa gaaataacta acgtattcat
    18301 tccagttctg taatcatcat tctccacttg cacatgtgca tgattaccat tccaattttc
    18361 tctgtacgga ctattctctg cttcaattgc tttcttataa actttagcca aacttgattc
    18421 tacttcaata ctaagtaccg cttcatgaaa atcattcatt tgtttactct cctatcagat
    18481 attttgtggt atgatttata aaaaagatgc gaggtgtttt aatgcttcaa gaagttaata
    18541 ttaagtatcg tgatcgtcaa aatacagaga caatttattt gccaaatagt tgtccgcatt
    18601 gcggaaatat aatgactccg tatgtgtatt ttggggtttc atcatatagt gcgttcgata
    18661 atgatagaat ctttggcgta ttagtacagt gtataaatga tgagtgcaac aaattttata
    18721 gtttacaatt tataaatacg tttaatgaag atgaaggata ttctgaaaat attttaatta
    18781 aagattatta ccgtccgccg attaaaatag atttaccaga aaatattgaa aaagtttctc
    18841 ctacatttgt tgatatttat actcaagcca cacaagctaa atctgagcaa ttggaacaaa
    18901 ttgctggagt tggttataga aaagctctag aatttctaat taaagattat gcaatatcta
    18961 aaaattcaga ggatgaagat aaaataaaaa aaatgttatt aggtaaaaca attgaaaatt
    19021 atttgaatga ttttcctaaa ctccaaagat tagccaaagc tgctacttgg attggtaatg
    19081 atgaaacgca ctatgttcgt agacatacta ataaagatgt tcatgatatg gatgcattta
    19141 ttaaatcagc agcgcaattt atcgcggctg attatgatgc tgatttagct gatcaattta
    19201 taaatggaaa taactttatg ccaactacta attaaaagaa taaaagagcc acagatacca
    19261 attaataaaa cggttgggat atcttggctc tttcatattt ttttgacact aatagaatat
    19321 catgctgcta acaatgttta catagctaca atgaccgaac atttagtgaa cagtgtaatt
    19381 ttagaataaa gagagttgtt ctttgtactt ctcgctattg taaaaatcaa tttcgttttc
    19441 ttttttcaat cggtcagctt gtctgtgttc atactcatct aaaaagttca atgtctttct
    19501 aatttctgca tgtcgctgtc tgacatacga tgagctatac ccaacagatt cagcaatatc
    19561 ttctaagctc atttgatcaa catatttcat cttaacaatt tgattatcga taccagaaaa
    19621 gctatctatc agcaataaca tttcttcttt ttgctcgatc aacaattcca attcattttc
    19681 tatcttttgg atattctctt ctaaagaaga agttctcgaa ttcttttcga tgcggacgtt
    19741 tgctaaatca ccacagaccc atcgattcaa ttcaagctta cttttattaa gattccactt
    19801 taagtaaaga atctgctcat ctagttcttg gtaatctttt aaccaatgaa atctcacaaa
    19861 cgccacccct tatggtagaa tagtattgcg ctgctatctc gaaaggggta gcttttttat
    19921 tttttcttag ccgttccatt ctggattgcc ttttgttcca aacgtcgttt tttcttctta
    19981 atctttgatt ttgtttttcc catacaattc tcctttccga aaacacattt tgttcgattt
    20041 ttaatactaa ataaaagttg gccagaattt ttcgttcgtc tttactcatc tttttttgaa
    20101 taagattact agcataaaaa tagttagtac cacgaaaatg aacaggtaaa tcaattacgt
    20161 tcaccttctt catgccagtt atcagccact agaatctttg cgttagtgcc gccaccgttt
    20221 tcttttttgt atttttcaaa tgctttataa gcagctgttt ttgatttata gatttttccg
    20281 gaaccgactg ggaacatcca tgtgccctct gtgatagcta agatgtatac tttcatttaa
    20341 ttcacccctt taatccccaa cactacatat ccggacttct gcatgtaatc agtaatataa
    20401 gtgatctctg ctttgtatga gtcaccagtg taactaaatg ttggtctcgt tagtccaggt
    20461 aattcatgtt taatgtattt tttatattca cgtagtatta actgatcacc aactttgaag
    20521 tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc tcgtcactgc ttcaaaatat
    20581 tctggtagta tttttagttc gtggatcatt ctgtttcctc cttcaaaatt cgctcatcat
    20641 caaaaagtga atctccacaa aatccttctc ccaaatattt atcgttcatg tataagtcaa
    20701 ccaaatattt gtgatctgct tctcgatatt cattaatatt aacgatcttt attttgtaaa
    20761 cattatcttc gtcgctcact atcttgaatt gttctctgac tttcaccacc gttcctcctc
    20821 tttttgaaaa aattttagtg tttttgtaag accccctaaa tcagcttttt tatttgatat
    20881 aatacttgta tcgattaatc aaatcgaaat taaatttaaa aaggtgattt atcatgaata
    20941 tcaacaattt agtaaaactc gttgaacaac gttttaaaga atcaggatta gaacaattaa
    21001 aaaaagataa ttctccagaa ttattttcta attttaccga agacgacatt cctactctta
    21061 aacaattaaa atattattct gattattgga cgcaaactcg tgagaatgaa ttaaatttag
    21121 aaaaactcat tattaaaaag aaatagcaac tccagcccac acggctggtt ttttagtttc
    21181 aacttccctc cttacgggtc aaaatcatcg gttccggcat aatctgatca tcctttagca
    21241 acaaatcagg atattttttc cttagcttct tattgttcgt gtaagttttc aatagccatt
    21301 ggttggcttc gcttttgctt tctccagaaa aataaatttt ccctgtttgg atgcctgcaa
    21361 gttgaatcat cattttcacc tcaaatcaaa tttttttctg ctagatggag caaaagcaat
    21421 ccgacatcaa gaatcttttg acccattatt tcagctactt tgctcggctt aactccttga
    21481 gaaaagagca agcgtgcttg ctctatctct tcgtgagtcc aaataaattt ggcttcctca
    21541 aggataatta ctggatcagt tgctctcatt gttcctctgg aaaaatgatg tcattcttat
    21601 gcttggacca ggtatctgca tatggaacaa agtattgtct agctaattcg atttgatcaa
    21661 ttaaagcagt ttctgataat tcatggtctg cagcaatttc attcatacta tcgccttcat
    21721 ctaaacagat aagaccgttg acgttatcga tttttacatt tgtgactgct tgactttctc
    21781 cacgctcata atattctaca gttgatactg atacatcagc ttcggagttt aattgcagaa
    21841 tctgttactg ccgggataat ttaatattct tttcataatt cacctttttt cctactgaca
    21901 tcgaatattt ttcatgttat gattagccgt gaaaggagag ctatattatg tgtttacaca
    21961 tgatgtcctt agctcattcg gttctttgga agcttttttg gaaataactc tcaggttctt
    22021 gacgcaaatc tcatttattc tttgaaacaa aaaacttcca ttttccgaga gctttggatc
    22081 acttcaaaag agatttgcat ttatatttct gtgaccttga gtcgcgtaaa gaaccttaca
    22141 gcaatggcgg caattccact ttcaggaaac cgcctttttt cattgatatt ggtggttagc
    22201 ggactaccca ttttctttcg gcagtgccaa accacaaatg atctgtgttg atgtttaggt
    22261 agttgtatac cagataagct aaatccaaag tgcctgcatc ttcaagacga caatattcca
    22321 aaaattctcc catagctgga cataaaaatc ctggagctat catatcccaa cagttaatag
    22381 tgcaaagaaa ttcgtttagc ttctgaacgt tttctttggt ttcacgaatt acaacatact
    22441 ccaattcttc tccgctggca gagcattcca aaacttcaaa ttgattaaga aatggtagaa
    22501 catatgcttc aaacatcaac attccatctt tctgtttttg cacttgtttt gcatcaatca
    22561 tgtctatcct cctattcagt tatttcttcc attaatggaa ttatttcagg tcactggact
    22621 taacgaaaac accattgacc atctttccag tccgaccttt gatttcgtca taagcaaaat
    22681 ccaagcactc gtataaatcc atatcatttt gcattgccaa gatgatcaag gttactacta
    22741 catcgccaat tccgtctctt aaaccatgtt catcttttct agctagagaa gcggcaactt
    22801 ccccgatttc ttcgaccgtt tttaacattt gcttgctgaa atccgcttga tccaatccct
    22861 tatcttttgc ccactgctct actttcgcga ttagttcgtc cattatttct cctccacata
    22921 cctaaactgt cttccttttg aatcaatcca taagctccta gctctatccc aaataatatt
    22981 tttgctcaga ccagtaattt cagataactg tttagcggta cccgttacta gaattcgatc
    23041 accatgccag attgcaatct ttctcggcgt tctccgtttg ggcttttcag tccacattga
    23101 tttaccgagc ttttggactt ctgcaactat ttttttatct tcctgccaat cttctgaata
    23161 agtcaattcg ataattcgct gcattgccgc tttcttatcc atcccgacat tctcctttca
    23221 gtaatttgag tacttgatca agtgcgctct cacgtccgcc atgaaatgtg ttgagccact
    23281 tgtcttcgta cgacacactt tgtcttaaag cttcttgatg cattaattcg atctgtgctg
    23341 taaatgtctt taggtccatc ttgtttacac ctgctcaagt tcattaagat gtttttgcaa
    23401 gcctttaaca caatcaatga atagtaattt ggtatatgct aaatttctta attgcgttcc
    23461 atcgatatag agtgcgaaat agtatctgag tttactccaa ctcgaacgat cattcttaat
    23521 tccttcaatt ccagcttctt cgagttgatc atatacgtct ctcaggacgt ctaattcctc
    23581 gcccttttta taattcgcta tttcattaat tagttctaga taatcgatct tcatttttcc
    23641 acctcttaga atggtgcttt tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga
    23701 taggcttctt ggtcgattgc ccattcagga agcttctctc gtcttcctgt gcgcttgtat
    23761 ccactgcttg cgttcttagg ttcacttttt tctttccttg cccaacttcg aatagttgcc
    23821 aaatagtttt tataagtctt accagatgat tcacaatact ctgacagtcg ttcgattcgc
    23881 tcttggtaat cattagggaa ttctgttttg agtttctcca tctgctcatc tgacaaaaga
    23941 acatttttat actctccgta tttatgacgg acgggcttag ccttcgattt tttcgaaggc
    24001 ggtaactctc ttatatattc ttttgtatta ttaaatgtat tattaataga tgtattatta
    24061 tctttgactt tttcgtcaat aggggtattg cgtttttcgt caatagggta ttgattaatt
    24121 cgtaggtacc tattgattat ttgattggta ccctctttgt aaatgatttc ccgattcaag
    24181 tatccaaact taatcaaatc acttacccat cgcgatatgg tctctttatt cacaccatat
    24241 aaatctgcaa agtactcatt gcctgcccaa caaaagcctc tttcattaca caaggccgtt
    24301 atctctccgt ataacaactt agtatttggt ttaagtcttt tgtcgtacct tacgttggct
    24361 ggtataatcg cataataact tcgatgttct gtcattttta ccctccaata tttaactttt
    24421 tgattgtttt ctggtttaat ttgatccctt tgatttgata tttatttttg aaattaatca
    24481 cacctatttt gtgcttctct gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg
    24541 catgatcaac ttctttgcgc tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag
    24601 ctcctgtttt gccacagatg cagcagactc tttttgtgat gcatttgtag aagtaatatt
    24661 cttgattcgc aggtaaaatc tcatagcctt ctttgaaagg aatatgatgt tcaaagatga
    24721 aatctaagat gatatttgct aagacattgg catcactcac agttgtattc gattcgtctt
    24781 tcaggcttat tttgcgccct gtgacacctt caaaacgaaa gtagaagaat tccttccaga
    24841 agtccgttgg catgcctgta tcgataaaaa tatcgcctat cagcgcatag atgaagtttc
    24901 gttgctgtac ggtgaaacgt ctaggatcaa taaaacgaat ttcaatgact cgatcaccat
    24961 cgtagccgtc atacatcgtc tttagtcgat caatgttcac ttcctcattg atggttgcgc
    25021 ttatgtcttt tcctttgaac tttttcagaa ccgctgaata tgaatcgatt aatggtttaa
    25081 acactcatat cacttcttat ctaattcttt tctcttagct gctattgctc gctccatcaa
    25141 ggcacattgc tcatagctta actgttcaat agtttcaacg ttatcagcta agagccctaa
    25201 tttatctgtc tgctcattaa catattcgat taaggttttg gtcatatctt tacccatctg
    25261 ctcattgaaa gcttctagaa tcgtctctag catgtttaat ttctttgtat cgattctagg
    25321 tggtgttgga atatcttccc cttgaaatac atataatccc agtccgtgta gagccaatgc
    25381 tttcacaaag catcgcttca atgagttatt gatttgcatt gcatttggtt taacaactgg
    25441 ttggtttcga taatctaaaa caggaaataa ttcggtttcc gtgtgtcctt taaccgttac
    25501 tgagacagat acataagtcc cagtttcatc cataagaaaa ggtttatatt cctcaacaag
    25561 aaagtcttga tgagttccag aaacaaccct gtagtgtttg tactcattaa tagttaccgt
    25621 tgcctgtgga tcattctttt tcataatctc ccacgcgtga gcccaagata aataatcaaa
    25681 atttcctttt ttcttgagaa ttttatttaa cttacgacta aaaagttttt caaagttcgt
    25741 tgtccctttg atttcactca tcaaattctg cctccatttc agcaatgtat ttcttacctg
    25801 gtccgtaata agagatatca atcaagttat ctctgtcgta ctcttctagc gcatcaatca
    25861 agccatcttc gatgacgtaa atatattcag gtttattcga atgcttcgat agatggataa
    25921 gataaacatg atcccaaata gttacatggt tgcctaaatc atcttgatcc caagctagtt
    25981 cttcattcgt caaaagattt cgtctgattt ttcgaccact tgtttcctca attttcggct
    26041 tgccccaatc aggatcaatc aaatattgat ctagagtgga aagttctttt tccatatgct
    26101 aaaatctcct tatgatgtgt tttctttgtg actctttgct tgccggcgga gtcacttttt
    26161 tatttgttgc catgcttttt gcttgtcaat atgttgttgg cttaggatga ttggtttata
    26221 gtatttccac cagcaattag caattgccgt ccctattctt agcgcttcag ctctattcat
    26281 tgtcatcacc aaaaagtctc tgttgtctgt tcagttgatc aatttccata cggatcgcag
    26341 tttctggcaa ccacatttca ataaatgaaa cagcatcatc gaatctctta cgaggtaact
    26401 cgccatatct tgggattgaa aaggtacgtt taaattcaga ccaaaatttt gagaatactt
    26461 ttttgctgat ttcttcataa gctcggcttt cttttccccc tagaactccc ataactttca
    26521 tatttccttt ttgcttaatt tcaaactctt gttgtccgct aattcgcata gtatctttaa
    26581 gcatggaaac atctttttta acatctttca tttcttctaa ttgatagatc atcatatctt
    26641 caattgtttg aggaacagta ttcttgcgaa taacatcttc catttcgttg aatgcttcaa
    26701 tgtatttttg tttgaagtag atagctttct ttcctgtaaa ccccatagcc agcaagaaaa
    26761 atccatctct actaatgaag aaaactcgtc gatttctgcc atatgaatct ggttcattac
    26821 cttctacaaa catctgtcca aaattggaca catctttttt tagtgcatca atatctctta
    26881 aaacatgttg atgttttttc tcgaagcttt ctgccacttg taagctcgta gtcacagctt
    26941 ctttattttt caaaattact aattcttgca ttatttcttc tctccttttt ggtataattt
    27001 agttaaaaac tggtggtgtt tattttgaat ttttttcact taacaatttt tcaatggatg
    27061 tcaataataa gttttatatt gagtacgttt ttaacaggga taaaaataaa aggaatgcgc
    27121 cctcaactag aagttgaact taatgcttct tacttcgctg cagatatgat ttatacaaaa
    27181 gtaatcattt ctaactattc aacagaacca gctatgttag ttaatttaga actttcatcc
    27241 gctaacttaa accgcagatg gtcagctact ccatttaaga aattaattgc taaaggagga
    27301 tcagtcgatg ataatagaat atactctgaa gctgtaccgc taaatattcc tcctaaaagt
    27361 gctatatctt gttaccttgc atttgaagta gggaaaatta attttaataa actccttaat
    27421 caccaaacaa aaatgatatt tactctaaat agaacccaaa tccacaaaat tgttgatatc
    27481 aaaaatacaa acttcccaat agagaaatta gtgaaagaat taaactgagt aatagtaata
    27541 tacattctag tttctctttt ctattcattt ttagtcagcc ccctcggttg gctttttcgc
    27601 tctgtactca gcttcagctt catcaagccc cataaaaatc caaaccatgt aaacaatcgt
    27661 tcctatcaac gcttgcctgc ttccccaaag tcctaaagcg tagatgatta gcggtgcgct
    27721 gaacactagc gctctattaa attttcccat ccgcttgcct ccttaagatt tccgaaaaat
    27781 ttgtttctaa aaattcaagt gttttacttc ttaaaaatag atatgtgtct cttccttcaa
    27841 ctggataata gacaaatcca tttttgtttt tctcgatatc gataatattt ctatatcttg
    27901 ggttctttaa aactctagaa gtaaaccaat catattttct gttaatccgt tctagcactt
    27961 ctggcaacgt catccatcta ccagtatcat cagctttttt taactcctca taatccactt
    28021 gggagataat tacatagcct tctggaattg ggatttttgc ttctagatat tgcattagct
    28081 gttcctccct ttacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg
    28141 cagaagtctt tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt
    28201 cagcaagcat ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc
    28261 tatctcgttg agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc
    28321 ctctaaatcc atttgagggt aatatccttc tttttttagt aattcgtaga taaatagacg
    28381 ccctttctgt gtccatttgg tattcattac aattttagtg ccaccatcgg ctttcgggat
    28441 ctcagttgta tgagattttg tgtatccttg gttcatgtgt tttttgcaca ataaccattg
    28501 gttaccgact tttttctgaa tacctagttt atgaagtaat ttattcatct gttgtggaga
    28561 catcccataa tctgctgcaa tctgactaat tgttactgaa tctgtagaag ataatatgct
    28621 atctaaatag gagattttag gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc
    28681 agttctttct tcttttagtt gagttgctaa ttgaatgatt gtatctggat taagcaaagc
    28741 ttcttctact ttttctggag ttagataacc tccatgtttt ctaattgctg gcaacacttc
    28801 acttgttacc caacgtttaa attttttggc agagggaagt tttgatttta agattaaact
    28861 gtacaaacca gattcgttga tgattgtcat ctctcttgat tgacctgagg tcgtgattcg
    28921 cgaccccatc ttatcttcca aatctacatg ccgcgacaaa gcatctttag tgtttgaata
    28981 acccaaaaca ctcgcaacgt ctttgcctac aaaatatggt tcatcattta ctaaaattgt
    29041 ccgaacttcg ttttgttcga aattaaaaat ttgcggtgtg ttcatatttc tcattccttt
    29101 cttttatatt cgtaaacaaa tttaacaact tttttctaaa ttcaattgac agtatctgag
    29161 taatactcta tactaagagc ataattaaat aagacataaa aacattgatt ttaaagcttt
    29221 cttggcggtc tgcatttaca tatcaatagt gttttttgtt gtctttttag ttgttaaatc
    29281 tgtttacaag atatagtata gctcattact cagaattagt caaattattt ctgcgtattt
    29341 ttctaaactt tttttgtaaa catttagaaa ggttgtttta tcgatgaata cctatgaaat
    29401 aataaaagac ttggcgaaga ggaaaaagat atctatacgg cagttagaaa ttaattttgg
    29461 atattctaac ggttatctag caaagtggaa aaataatact cctaattcag atgaattaag
    29521 tcggattgct gactatttcg atgtctctgt agactacctt ctaggtagag aagaaagaga
    29581 aacccctaaa catgtggatt tatcagaaga cgatactgtt ttttcttttg atggaaaaga
    29641 aatatctaag gagacaatgc gtaaagcgat tgcaattgct aaagctttag aggaaaatga
    29701 atagttggag tgatggtttg tatgtatttg aaattgaaag aaatgttgag cgaatacaat
    29761 ttgaaagtag tttatatgga gatgaaagag cctggtttct actatccaaa acccagaatc
    29821 atttttttga atgaaaatct atacggtgaa actgcagaag cctttcattt atctcatgaa
    29881 cttgcacatt tcagcgcctc tcattttgaa ttttcagttt tgtacgatac ttctacaact
    29941 tttcactcaa aatttgaaac agaagcagat aaaatagcta ttctaatttt attaaatatc
    30001 tatattgaga acgaactgac tgatgaatct cagtttaatc tcgaaaaatt tatggagtat
    30061 tattctattc agaataagct cagatacact tgttatgctg tctgccaatg ctattttaag
    30121 aaaaaatatt cttacgcaag gcaatatgta tgaatacatc aagaattatt aaaaaaaccg
    30181 atttcagcaa aacattaaaa aagcccgtgc tgcaacacgg gctctttcct catatatgag
    30241 cttctacaaa aaaaatcata tcacagaaat gaggaagaaa aaatggaaaa agagatttta
    30301 ataaaatctc caggaaaaac cttgattaaa gtaac
//
LOCUS       DAFGFZ010000022        30242 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000022 DAFGFZ010000000
VERSION     DAFGFZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.022"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            19..717
                     /locus_tag="LR726_000842"
     CDS             19..717
                     /locus_tag="LR726_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL6215950.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(885..3524)
                     /locus_tag="LR726_000843"
     CDS             complement(885..3524)
                     /locus_tag="LR726_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL6215951.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4143..5174)
                     /gene="queA"
                     /locus_tag="LR726_000844"
     CDS             complement(4143..5174)
                     /gene="queA"
                     /locus_tag="LR726_000844"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL6215952.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5367..6542
                     /locus_tag="LR726_000845"
     CDS             5367..6542
                     /locus_tag="LR726_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL6215953.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            6546..7187
                     /locus_tag="LR726_000846"
     CDS             6546..7187
                     /locus_tag="LR726_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215954.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            7184..8101
                     /locus_tag="LR726_000847"
     CDS             7184..8101
                     /locus_tag="LR726_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6215955.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            8098..8769
                     /locus_tag="LR726_000848"
     CDS             8098..8769
                     /locus_tag="LR726_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6215956.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(9310..9798)
                     /locus_tag="LR726_000849"
     CDS             complement(9310..9798)
                     /locus_tag="LR726_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL6215957.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(9999..10265)
                     /locus_tag="LR726_000850"
     CDS             complement(9999..10265)
                     /locus_tag="LR726_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL6215958.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(10265..10528)
                     /locus_tag="LR726_000851"
     CDS             complement(10265..10528)
                     /locus_tag="LR726_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL6215959.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            11004..11189
                     /locus_tag="LR726_000852"
     CDS             11004..11189
                     /locus_tag="LR726_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL6215960.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(11331..13154)
                     /locus_tag="LR726_000853"
     CDS             complement(11331..13154)
                     /locus_tag="LR726_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL6215961.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(13752..16457)
                     /locus_tag="LR726_000854"
     CDS             complement(13752..16457)
                     /locus_tag="LR726_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL6215962.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(16763..17710)
                     /locus_tag="LR726_000855"
     CDS             complement(16763..17710)
                     /locus_tag="LR726_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL6215963.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            17900..18169
                     /gene="rpsN"
                     /locus_tag="LR726_000856"
     CDS             17900..18169
                     /gene="rpsN"
                     /locus_tag="LR726_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL6215964.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(18345..18707)
                     /locus_tag="LR726_000857"
     CDS             complement(18345..18707)
                     /locus_tag="LR726_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL6215965.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(18718..19839)
                     /locus_tag="LR726_000858"
     CDS             complement(18718..19839)
                     /locus_tag="LR726_000858"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL6215966.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(19855..20205)
                     /locus_tag="LR726_000859"
     CDS             complement(19855..20205)
                     /locus_tag="LR726_000859"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL6215967.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(20335..21846)
                     /locus_tag="LR726_000860"
     CDS             complement(20335..21846)
                     /locus_tag="LR726_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL6215968.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(22168..23520)
                     /locus_tag="LR726_000861"
     CDS             complement(22168..23520)
                     /locus_tag="LR726_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL6215969.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(23673..24749)
                     /locus_tag="LR726_000862"
     CDS             complement(23673..24749)
                     /locus_tag="LR726_000862"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL6215970.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            24945..26690
                     /locus_tag="LR726_000863"
     CDS             24945..26690
                     /locus_tag="LR726_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL6215971.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(26970..27407)
                     /locus_tag="LR726_000864"
     CDS             complement(26970..27407)
                     /locus_tag="LR726_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL6215972.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(27391..28764)
                     /locus_tag="LR726_000865"
     CDS             complement(27391..28764)
                     /locus_tag="LR726_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215973.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(29086..29574)
                     /locus_tag="LR726_000866"
     CDS             complement(29086..29574)
                     /locus_tag="LR726_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6215974.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(29596..30198)
                     /locus_tag="LR726_000867"
     CDS             complement(29596..30198)
                     /locus_tag="LR726_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL6215975.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         8668 a         6465 c         5620 g         9489 t
ORIGIN      
        1 gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa aacgccaagc aaaagagagt
       61 ctaaaaggac gttggggaca agctgtttta ctaaatctaa tccctacatt gattggtatt
      121 gctactattc ttcttattat gattcctatt atcttgtttg ccaccaccat atatgatgga
      181 tcagcgagcg tctcagaagc gacaacttca ggaggcggta gttcaggagg cggtggcgga
      241 attatttcca gcatcattag cgctttgttc atgagcggta tttcttggac ttatcttgat
      301 attttacgtg gacaaaaaga tcagatcgaa ccattcaaag atgcatttcg tggattcaaa
      361 ggtttgttta tcggcggagt gatcagctta gctgtgctga tctcgatctt tacaacatta
      421 tggggtttgc tattagttat tcctggaatc attaaaagct attcttattc tcaaagctat
      481 tttatttact acgatatcgt ccaagaaaca ggacaaacac ctaaagtact ggatacgatc
      541 actgcaagtc gaaaattgat ggatggttat aaaggacaac ttttctggtt agatctttca
      601 tttatcggct ggcatatctt agcattagca actgctggta ttggttatct atggctcagc
      661 ccatacattt ctgcaacaaa agcagcattt tatgaacaat tgccaaaaga tatttaaaag
      721 agagtgtgac ttgtgaccga gggggtggca ggagaacacc gtttatcgag aggcagtaaa
      781 agaaacattc agcccattag caaaaaggag gtgtgacaac attcatgtca cacctccttt
      841 ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag ttaatcattc ctcttcttct
      901 gcttccggta atcgatggaa cttgatcagt tcattcaccg cgattggtac gatagataaa
      961 atccctacca agaaccagtg attcaaactg atgtgtacta ggccgaatag ttcttgcgta
     1021 aatggcagca aagcaacagc cgttgtaata gctaatgcca gtaacgccat ttctaccaaa
     1081 gctttattgg tcgctaaatt gactttgaag atcgattggc tgctgcgtac attgaacaca
     1141 tgcagaatcg atgaataagc taagatcaag aatgcaacag tttgaccgac atgctgactt
     1201 gcgtctaccc atgggctgat agtatcgaca taactaccta aataataacc aaacaaggtt
     1261 acgatggtga agacccctgc atttatggca attttttgca ataaacctcg agcaaagatc
     1321 ccttcatttt tcggaatggg tggctgatcc atgatgccgt gttccgctgg ctctcttcct
     1381 aaagcaaatc ctggaagtcc atcagcaact acattgacaa acagtagttg cacagctgta
     1441 aatggtgctc cccatcctaa cagcatcgca atcaaaacaa taaagatctc cgaaatatta
     1501 cagcttagca aaaagttgat agctttacga atattctgat aaaccgaacg tccttgactg
     1561 acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa tcatatctgc ggcactttgc
     1621 gccacatctg ttcctgtgat ccccatggca cacccaacgt tacttgcttt caacgctggt
     1681 gcatcattca caccatcccc agtcatcgct acgactgcac ccgtacgttg ccaagatttg
     1741 acgatccgga tcttgtcttc tggcgttacc cgagcataga cagagagagc tttgacacgc
     1801 gcatctaatt cttcgtctga taattgatgc agttctgatc ctgaaagtgc ttctgacgga
     1861 tcattcaaga tacctaattc tttcgctatg gcactggcag tcaccacatg atctccagtg
     1921 atcatgactg ttttgatgcc agcctttttc gctcgcttga ttgctccttt actttctgga
     1981 cgtggtggat cgatcattcc gatcagacca atcaaacgta agtctttttc cagtgcttca
     2041 gaagaaatat cttgcggttc ttcgtcataa gtagcataac caacagcaat cactctcagt
     2101 gcccgtttac caaagcgatc attaacgatc gccgcttgtt ctacatcgcc tgaactaaag
     2161 cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga tgtatttttc gcccatttgg
     2221 tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa gctcgtttac tcgtggatat
     2281 ttttcttcta attctgcctt tgtatgataa ttctcttcaa cagcccttac gatggctact
     2341 tctgttggat tacctttgat aacaaggtcg tcatcttctt gctcgaccgt cacatctgta
     2401 catagtgcag ccattttcaa tatttccatc gcgctgttcg tcattgaatc ttcgatattc
     2461 gttacttcat cttctttgct ccatacccgg cggacgcgca tttcattttg tgtcagcgtt
     2521 cctgttttgt ctgagcagat aacattagcg gttcctagcg tttcaactgc tggcaactgt
     2581 cggatgatcg catgtttcct cgccatcttc tgcacaccgt atgcaagtgt caacgtaaca
     2641 ataacagtca aggtttccgg tacagctgca acagccaagg aaatcgaagt catgaacatc
     2701 tcaaggagtg gttctccttg cagttcacca atcacaaata ctaaagccgc agccgctaaa
     2761 gcgatcatac tgattcgttt gcctagctga ttcaatcgga cctgtaaagg tgttttttgc
     2821 atagtatttt cgttcaaaag atccgcgatc ttacccattt ctgtagccat gccaatggca
     2881 gtgacgatcg ctttcccacg acctgcagca accgtacatc ctttaaatac catattaagt
     2941 tggtcaccta atgactcatc ttcttcagca ataaatgctg catctttttc aactgcttca
     3001 ctttctccag tcaacgccga ttcttctaca cgtaattgtg atgcttcgat caaacgggca
     3061 tcggcagaaa tagcatcacc ggactctaaa acaagaatat cacccactac cagttgctct
     3121 gactcaacag tggtcacttt gccgtctcgt atgacagttg ccgtttgttt attcatatct
     3181 tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga caatcgccaa taccgagttg
     3241 atcacaacga tagcgatgat cagcagtcct tcaaataaat ccccatgttc tgtagcaaat
     3301 gcggtataaa aggaaatcac cgctgctaca agaaggatga tcgttgtaaa atcggacaag
     3361 cttcgaaaga attttttgat catcgattct ttcggcgcct catcgaattt attcaatccc
     3421 tgcttttttc gccgttcatc cacttcttgt gaagacaacc cagtatctgg atctgcttct
     3481 gttaccttct ttacttcttc tattgtctgc ttgtattctt ccatcctatt cctcctttaa
     3541 aatcttcgta caaaaaaagg tacttatttt actataacat ccttttttta tttacttcaa
     3601 ataaagggct tatcgattat ttgttaagaa agcgtaaaat ttaaagtgct tatatttgca
     3661 aacgctacca aaggatgcta ttatatagat gtaaggaagg aggataccat caaatagaca
     3721 gaagaatttc tcgatagaac agtgtaagca aacaaagttg ttagaagtac aggtttcacc
     3781 cagacatgcg tgtagagtac aaacctataa atagtgaatg cgtatggatg tttggatcac
     3841 gaagtatccg aaacaagaag taatgcgata cgtaacaatt gaataacgta aacgtctgaa
     3901 aattgaatag gaacaagaga tgtttcaaat gttgaagatg taacgaaaac aaaatttttg
     3961 tttaacatga agaaattcaa caccgtgctt tgcatgtatg atggaaagtt acaatcattt
     4021 ctgtttattt gaatccttcc gaggtacaag aaagccgagc ttatcgagag ctcggctttt
     4081 tctttgtcat aaaatctttg taaagctttt ttgttattca aaactagcgg cttttcataa
     4141 ccttatttca cgaacatcgc atctccaaaa ctaaagaaac gatagcgctc ttcaatcgca
     4201 tgctggtaag cagataaagt caattctctt cctgcaaagg cactaactaa catgactaaa
     4261 gtcgattttg gcaaatggaa attagttgag aaagcttcta ccactttgaa ttcatagcca
     4321 ggcgtaatga aaatatcagt ccatccgctg tctgctttga tttctccatt aaatttcgtt
     4381 ccaatcgttt ctaatgttcg aattgaagtt gttccgacag ccacgatttt cccgccattt
     4441 ttacgtactt ggttcaatcg ctcagccgct tcttccgtca aacggtagaa ttcactatgc
     4501 atatggtgtt cttcaatttt atctacgctg acaggtcgga atgtacctaa ccctacatgt
     4561 agtgtcagat agactaattc aacacccttt gcttcgatct catcaagtaa ttctttcgta
     4621 aaatgcaatc cagctgttgg cgctgcagca gaaccatttt ctttcgcata gacagtttga
     4681 tagcgatctg ggtcttccag acgttctttg atatatggag gcaatggcat ttctcccaat
     4741 gattcaagaa tctctagaaa aattccttca tactcaaagt caatgatccg tccgccgtgt
     4801 tccaattctt ctttcactac tgcttgcaat ctgccatcac caaaagagat tcttgtccca
     4861 actttggcac gtttcgcagg tttgatcaat gtctcccacg tatctccttg tgtattcgtt
     4921 agaagaagga cttctaaatg cgcaccagta tctggttttt ctccatataa acgagcaggc
     4981 agaactcgtg tattattcat aactagcgca tcaccaggct gtaactcttc aatgatttca
     5041 tggaaatgtt tatcttccat ctctccagtt ttccgatcca aaacaagtaa acgagaatga
     5101 tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg gcaagtcaaa atcaaaatct
     5161 tctgttgtta acatatcgtc actcactttc tatcatgtgc ttcatcattc tatcatgttt
     5221 ttcgttctgt tggcaaaatc agcacttttt cacttctatt ttttgttttc ctaaataaaa
     5281 aaactaccct atttgtattt tttcattgtt ttaggcatac tagtaataga tagttcttca
     5341 ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca aaatgtgtca aaaatttata
     5401 aaggtggaaa agtcgctgta gaggacgtca atctttcatt tgaaaagggt gagttcattt
     5461 gttttatcgg tacgagcgga agcggaaaaa cgacgacgat gcggatgatc aaccgaatga
     5521 ctgatccaac acaagggaaa attttgattg atggaaaatc catccaagaa attgatccag
     5581 tggaattgcg acgtcagatc ggctatgtta tccaaagtat tggattgatg cctcacatga
     5641 cgatccgtga aaatatcact cttgtccaaa aattgttgaa agtcagtcaa gaagaacgga
     5701 ataaaacagc tgaaaaaatg attgatcttg tggaattgcc ccgtgagatg ttagatcgct
     5761 acccacatga attatctggt ggtcagcagc agcgtattgg agtcgttcgt gcccttgcag
     5821 cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca atcactcgtg
     5881 attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg atcgtatttg
     5941 taacccatga catggatgaa gcgattaaac tagcaagccg tatcgccatc atgagtgaag
     6001 gccgtgtgat ccaatttgat acaccagaaa atattttacg tcatcctgca aacgatttcg
     6061 tagaagagtt gattggagaa gatcgcctac tacaagccaa gccaaatgcg acaagagtcg
     6121 gcgaagttat gctaaataca gcaatcacga ttacaccgga aaaatcatta caagaagcaa
     6181 tcaaactgat gagggaaaaa cgtgtagata cgttgttagt taccgataat tcgaatgtat
     6241 taaaaggatt tatcgatgta gaaacgttga ataaaaaacg aggaaaagta tccagtgtcg
     6301 gcgacatttt gaataaacat gttttttacg taaaagaatc cgcctatctt cgtgatacct
     6361 tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt cgttgatgaa cagaacaaag
     6421 tcgtaggtat cctgactcgt gcatcattag tggatatcgt ctacgatgtg atttggggag
     6481 aagatcccgc aactactgat gttgcagagt cagctactcc tgaagaatct aaggaggtct
     6541 gacctatgca gcagtttttt catcagcacg gttcagaaat tctctcaaaa ggttttgaac
     6601 atctttatat ttcaggactt gctttgatca ttggtatcat cattgctgta ccgcttggtg
     6661 tcttactgac acgttttcca aaagctgcca cgattgtgat tggcttaacc agtgccttgc
     6721 agacaatccc ttctcttgct ttattggcat tgatgatccc gctatttggc gtaggtaaat
     6781 tacctgcgat tatcgcactc tttatttatt cattattgcc gattttacgt aatacgtaca
     6841 ttgggatgaa aaatgtcgat tggaattatc gggatgtggc aaaaggcatg gggatgactg
     6901 agttccaatc cattttttcc gttgaattgc ccattgctat gccaacgatc atggctggta
     6961 ttcgtttagc tgccgtttac gtgattgcat gggctacact tgcctcttat atcggcgctg
     7021 gtggtttagg cgatttgatt tttagtggac tgaataatta ccaaccagat ctcattttgg
     7081 caggtacgat ccctgtaaca attttagcac tattggctga ttggcttcta ggtctcttgg
     7141 aacaccggct gacaccgatc gcacttagag aggagaatga agaatgaaac gtatactaaa
     7201 aatgaccatg gttcttttct ctcttcttat acttgcgagt tgttccttcc ctggtcttgc
     7261 cagcaataca gatgaagaca ccatttcgat cacaggtgga atcacttcag aagcacagat
     7321 cttagcaagt ctagttgccg gaatggttga acattataca gataaaaata ccgcaatcat
     7381 caacaatctg gctactacaa ctatcaacca tcaagcgatg atgaacggag atgcttcaat
     7441 ttcagcggct cgctacacag gaacagattt aacgactaca ttgaaccttc ctcctgaaaa
     7501 agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt gagaaaagat acggtcaaac
     7561 gtggttcccg tcctatagtt ttgaaaatac gtatgtcttt cttgttcgaa aagacaccgc
     7621 acaaaaatac catctatcta aagtcagcga tctgaaaaat gtagcggatg aattagtagc
     7681 tggtgtagat acttcttgga tcaatcgtaa aggagacggt tatgacggct tccaagaaac
     7741 ttatggcttc tccttcaatt cgatattacc tatgcagatt ggacttgttt atgacgcagt
     7801 cgaagcagga aaaatggata ttgttcttgg ctactctacg gatggacgga tcgccagtta
     7861 tgatctagtc atgttggaag atgatcgtca attcttccca ccatatgatg ctgctccagt
     7921 tgtagacaac aagatattag ctgatacgcc tcatttaaaa gaagccttaa ataagttagg
     7981 tcataccatc agtacggaaa aaatgcagga attgaactac gaagcagata ataatctagt
     8041 ggaaccatca gtagtagcac aacgtttctt acaagaaaac cactattttg aagggaagtg
     8101 aagcaattgg ataaaatgaa tgtctttcaa caatttttct attatttcca ggaaaatggc
     8161 tcctatatct ttgcacaatt tatccgtcac ttcctgattt ctatctatgg tgtcctcttt
     8221 gcagcagttg tcgggattcc ggttgggatc atgatttcta ggagacgtaa attagcaaac
     8281 tgggtgattc gtttggcgaa tatcatccag acgatccctt ctctagccat gatctccatt
     8341 ttaatcattg gattaggcct gggagtcaat gtcgtgattg ttactgtatt tttgtattcc
     8401 ctcttaccga tcatcaagaa tacgtatacc gggatgatcc aagtagacaa aaacatcctt
     8461 gatgtcggca aaggcatggg gatgactgct agacaacgac tattcatggt agagttacca
     8521 ctctctgttt ctgttatcat ggcaggcata agaaacgcgt tagttgttgc catcgggata
     8581 accgcgatcg gtgcattcgt cggtgctggt ggtcttggag atatcattat ccgaggaacc
     8641 aatgcaacag agggcacatc gattatttta gcaggtgcat tgccaactgc attaatggcg
     8701 atcatcacgg actggctttt aggtattttg gaaagacggc tagatccagc cagccgtaca
     8761 tcacgctgat gttattgtaa aaaacaaaag cttctgcgac catttgttgg tcgcaaaggc
     8821 ttttttagtt ctgtcatttt taaggcaata atccactctg attcttccct ccttatgggc
     8881 aaagagttgc ttttcttagc tatttatcca ttccgtatac tccaacttca tactgccaac
     8941 gggttcatat ctgcatccac cgactaagtt tttttcttat ccgctaaggc attttgccaa
     9001 tccattttcc catcactcgt catggaattc ataccacatg atctctaaat actcgtctgc
     9061 ttgttctttg aattgggcat caattcttac atcctttggc gtggctttca tttaagcctt
     9121 ttccatcagt cctcgattgt tcgatgcctt cagctgcatc atcaaaactg gtgactgccg
     9181 tatcggtaac ttcgatcatc ttttatacaa tcgggaactt gccatcctca tccttggcta
     9241 aaaacatatc tagccattcc agcttctcgc catgggaggt ttgcggttcc taaaaccggt
     9301 acacgattct tatggactgc atccgtcgca actagacttg gcgatacgat gatccttcac
     9361 cagaagagca cccccgtaaa ccaattgatc gatatattgc caataagaaa agacattggc
     9421 gtaaaagata tcgatccctc gtgatgcatt gccgttgata ctgctattca tggccgaaag
     9481 cgccacgact ttaacttctt ccgtttgagt gacattcatg ttcggcaatg tcgctttgga
     9541 tacccgctct gctaaaggaa cgacactggt attgtatttc gtatctgtat cttttccaaa
     9601 agaccatgcc aacgaatcct ctgggaacca ataagacgac tccggttgat tgtccattgc
     9661 ttttttcaac atacttttgg ctgtttctcg ataaacgaca tttccaattg tcttcgcctc
     9721 actttggcga taatcgatgg tcgttgccac tgccaacact aacaacgatg ctccaatttt
     9781 actgattttc cttgtcacac ttcttcctcc ttgttttaca taaacccgtc aacattccat
     9841 tcctaaactc aaacaatgac ccttcaaagg tggagatata ggaacaaatt aaagaaatca
     9901 tgcaataaat ccaagaaggt tggttttaga acaaaaaaag agtctccttt ttggggagac
     9961 tctttgttaa ctatctaaaa aggttggctc ttctcacttc actcataatg tgtccaggca
    10021 gaataaattt ctacaacttt attttcctca tcaactttgt aaaccactcg atgctgtcta
    10081 ttcagcctgc gtgagtatct gccttcatgt gtaggtctca gcttttcaaa agactgcgtg
    10141 ggcatatatg gatcttcctt caatgtctga atcacttctt caaattgttt ctttaaattc
    10201 gtttgcttga tttttcttaa atccactttc gcagagtttt tgattgcgac tgtatagtta
    10261 ctcatcaaag agcttcccaa tcaatatcat cgatattcgt aaagctactg tcgtcttttt
    10321 cgcggtctcg tactttatct aaagtacctg tttgctcaag aaaaagggtt tcttgaatcg
    10381 ctctccagtc ttccagccca atcaatacag cattgtttcc actacgatca ctaataatct
    10441 cgatttcagt atgattttca tttacctctt tcaataactg ataaaaattt ttacgagcag
    10501 tgctaggatt catcgttgta ttagccattg tctctcctcc tttttctgat ataattatag
    10561 cgtacttgat taagtacgtc aaaaaaatac aataaagcac ttaaattcag caaacttttc
    10621 ctctttttta catagagaaa aagacccctt tttagggagg atctttgtcc acgctcactt
    10681 cttactcctt tacgactagc ggtagccaaa cgtaaagtaa ttggtaacaa cgtatcatct
    10741 ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac aacaccaata cacgaaggtt
    10801 cctcgttgca gtgtgacttg attgatatct tgaataacct gaggatacgc acgaaagatt
    10861 tgtaacggtt attcaaaact gattttggcc gtcaagtctt ctactgcctc aaaaatgatt
    10921 gcctctttcg tctcctgtca ttcatgttca ctatcgattt gatacttgct tagaccgttg
    10981 ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg taacaaaaaa tggtcacttg
    11041 acgaggctga gattcctact tatgaagaca catggcactt aagagtcgag gtaaaggaag
    11101 aagctgaaaa tataggaggc tgtacattat ttggcaaagg attcggaaat tatgatcaag
    11161 acatcaaaat caaaatgttt tacagctaaa taggaagaat aataacattt catacgctgt
    11221 tcccatcttt aatggcttat agagggcacc tttagctgag aataagacaa aacagaccct
    11281 ctagcttcaa ttaagagcta gagggtctgt ttttgatttt aaaacttggt ttatttcttc
    11341 aaatggtagc tataggtgct gacaacgata ttttcacgcc aagacaatcg caatcttaac
    11401 cgcaaacttg tttggttatg gagaatattc tgccagcgtc tgtttggtac aaattgagga
    11461 atcaacacag tagttgtata gttttgtttc gctgcatttt tacttacgat atctacataa
    11521 cggataattg gattttcaat cgagcgataa gaagagtgga cgattgaaaa acggacatct
    11581 gggaaatgct ttttaaattc tgcttcgatt tctttttctt tttcgacatc ttcgtctagc
    11641 gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg cgtaattcaa cgcaccgata
    11701 tttacccgtg tcacattccc gactaataca agaacggtat tcccgtcata atcatgtagt
    11761 tttgcatcat tttctaaacg aagctgttct gctacttttt tataatgatc atggatctta
    11821 taaaaaatga acataacgat tggcataatg atgaagaaag gccaaatgtc ccctaaccga
    11881 tagacaaaca aaatcgcaat gattgcatat gagatgaacg cacccgtaat attggcaatc
    11941 gatttgctta accatctttt tccttctttt ttccatttga cgaccatccc tgtttgagat
    12001 aaagcaaatg gaatgaacac gccgatagag tataacggaa tcaagcgttc tgttgagcca
    12061 tggaaaataa acagcaatac gatcgaacct agcgctagtg tgataatccc attggaatag
    12121 cctagtcgat ccccacgatc ttgatacatg tgaggcatga atttatcttt tgccagattg
    12181 tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag caactgctaa aatcagcgct
    12241 gtagcaaatt gaaggacgta atacaaaata ccatgtccaa agacagcttt accaatttga
    12301 gacaaaacag tgacttcttt ttcaggaacg atcccatacc aatagttgat aaatgtgatc
    12361 cccacgaaga agaaacctaa gatcaatgcc atcatcgtca atgtagcagc ggcatttttt
    12421 gctcttggct tcttaaagaa tggtacagca ttactaatcg cctccacccc agttaaagag
    12481 gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag aaacacccgg cacagccgct
    12541 cctggaagtg cagtagcttg caatggcaca gcacctgtca cgatattata taaaccaaca
    12601 acgatcagta acgagatcac aaggataaat gtgtacaccg gaaataatag gaacgatgca
    12661 gattccctca gcccacgaag attcagcatc atcagtagca aaacgattgt cacagaaata
    12721 gcgacttgat gaccgtagag tgctggcacc gctgatgtga tcgcttcagc acctgctgag
    12781 acagatactg ctactgtcaa catgtagtcg atcaataaag agccaccgga aatcaagcca
    12841 gcattctttc caagattctc actgctgacg acatatgctc ccccgccatg aggataagca
    12901 tggatgattt gacgatatga tagcgttaat gaaatcaata aaataataac aaaagcggca
    12961 atgggtagcg aataccagat ggctgcagca gacaaagcga ctaaaacgac aacgatttgt
    13021 tccgtaccat atgcaataga tgataacgca tcagacgaca ataatgccaa agcagcaaat
    13081 cgggttaatt tgtgctcatc attttcagca gattttaaag gcttacctac taacaacctt
    13141 tttaagtaat ccaatttcta aactccttta cacacaattt tacttcaagt tttatagtcg
    13201 aaaatttata gtaaacaaga tgttatagta cgctcactga ccttaaaagt caataaactt
    13261 taagaaaata attggaaaaa aacagaaaaa tatcaagcgc tttttcaaac gctttaatac
    13321 agcaaaaatc taacattttt ttatgggctt tttctttgaa ttctataata aaaggataga
    13381 acttgactta gaaaatcaag ttctatcctt tttgatatct taacttttgg aagagagatg
    13441 taaggctgct ttctacccat cattcttact actcgaagca tagcgcagtt gtcacaacct
    13501 cttgataaac ggtgtttaaa agcttgcatc tgcggtaata agcccaacca aattaaaaac
    13561 ataagaagct gtttttattt ggtcgttctt attacttgat gcagagcagc ttttttacaa
    13621 cctcttggta cacggtgttc aatcagctga ccctcggtat tagctcaaaa aaacggcaaa
    13681 atatgaggaa ctatttttcc atttttttct ctaatactca ggtcaaaaca ctgatttcac
    13741 aacctctttt tttaatgaaa aatatggctc tcatgcgccg cttctttgaa tttcgtcatt
    13801 ttttcttcga tgaacggata gattgccgtt cctacgattc caaagacaac aaacaaaccg
    13861 atcatgatcc agattgcttt cgtagcattt ggccaataaa ttcctcctgc agattcccgt
    13921 aacaagttta ccgcatgagt aaatggcaag aacggattga tcatttggaa gaatttccct
    13981 gagacttgga tcggatagtt cccgcctccg ccagagatcg acagtactaa gatgataata
    14041 gcgatccctt taccgacatt atcgaataat gcagccaaga catagacgat catcatgaac
    14101 gctaaggcga tcaagacagc aaatgacacg ctgtagaccg gattttggac atctactcct
    14161 agtaagaaaa tattccctaa tgtaacgatc aagctttgcg caatcgccat tgtcaaaaat
    14221 gtcagcatac gagcaacgaa ttgttctcgt ttcgtaaaat tgtttcggtc tttctttcct
    14281 aaatagtatt ctgtcgtagc aacacttgaa agaagaacag cgccgaccca cagacatagt
    14341 gcagtataaa acggggtgct agctgaaccg ttatttgcga ttggataaag cgcattggtt
    14401 tgtagctcaa caggttgtgt aaagaagtca ctttcttttg ttgcgtctag tttcaataat
    14461 ttgatgactt cgcctagatc gacttcttgt tcgccttttc ggatcgcgtc tgctgctttt
    14521 tggataccag ctttgatatt tggccagtca ttgttgatca agtccgcagc agcgttcaca
    14581 gctgtttcga cttctggcat tttttcattt actgttgtca atgtaccggt gatttcattt
    14641 tttagtgctg gccaatcatt ttggataaaa tcagcggcca atcctaattt ttgctgaatg
    14701 atcggtaatt cattgttgta taggtctgcc ccttcattga tgccattgac gatggtatcc
    14761 atatttccat tcagcattgt attggcatca tggatttctt gtccaattgc aggcatttct
    14821 ttttgatatt tttctaaaat tgccactgca ttcgttacag ttgctttcgt tgagctgaga
    14881 agcgaatcaa agtcgatctg gtccgctttg ctcagtgcgc cttgtgcagt cccaatcgta
    14941 tcgatgactt ggtctagtag agaagtgaca gtggaactga tttgacctgt attgatacca
    15001 gcaatgatgc cattgatatt attaacaacg gcatcaattt gatctagcaa ttcatcaata
    15061 gccttcgtgt cttcattttg aacagctgtg ttcaaagcag ataggcggtt ttgcagattg
    15121 cttagcaagg gttttaattg atttagctga tcaataattg gctgtaattt cgtatttcca
    15181 gcacttgttt gcaatttagt caggaactcg accagtttat cgattgcatt ttgttgttga
    15241 gatattccgt caataaagcc gttcaataca ttgctgatgt ttttcttttc atcatcagtc
    15301 agcttaccat cttcaatagc tgttcttaca gtatctatta acgaacctgt cgttgttgcc
    15361 acttgtccaa ttgacttcaa tgtaacatct acactgtttg tgatgcttgg tatcgcatct
    15421 ttcagctgag aggcacctga ttttgtttgg tttgctagat cacttgcctg attttctaaa
    15481 tttttcacat ctggcaagat atcttgtact tgttggatga tcgtcaagcc ttgttttgct
    15541 tcatcgattc cttggttcat tgtctcttgg atcgaagcaa aatcgccgtc gatctctgct
    15601 agttgcttac cggcattttg gatctctggg attttttctt gcaaatcaag gatgattttt
    15661 gcttgatctt tcaactcagg cattttgtca ttcaatgcaa cgactttttc acccatctca
    15721 tctactttag ggatgtaatc cacgaattca tttgctttat tcagtttttc cttgatttca
    15781 ggtagttgac tttgtaattc gaggacttgt tttgtgtagc cgtcgatcgt gtccaaattt
    15841 tcatctgttg ataaaatcat atctttaact ttattgatac ttactaggtt cgtatcgatg
    15901 tcgtaaccta tctcattgaa tactttcaat aaagtcgaac tggctgtttt gataaattca
    15961 tttgtgattt gcgattggat cgaacttgct cctttatcgg tgattttcgg tgcaatcgca
    16021 ttgatttttt catttaccgt atattcgatt tttggtttct tgatatcacc actagtaaaa
    16081 ctcagcagat cttctgagaa atcctttggc aagtaaatac ctgcgtaata ctttccggaa
    16141 cggactccgt cttctaattg ttcttttgaa tcaacgaatt gccatcctaa ttgtttattt
    16201 ttatgaagcg attcgatgac ttgctcccca atggccactt ctttaccttg aaactctgct
    16261 ggcttgtctg cactgtaaac agcaattggc agctctccag tatttccata tggatcccat
    16321 aatgctttga tgttaaacca cgcatatagc gaagggatga tcatgatcgc gatgatcaga
    16381 agtgttgcaa tcgggttttt gaaaatccgt ttccagtcaa gcaaatataa tcgaagcgta
    16441 tttttgattg actgcatagt tctccccctt cttctttttt cagctgaatt actttaccat
    16501 gtatttcata cttttttcaa tcggcttttt tcaaaaaagt cgaatttttg gacatatcac
    16561 ggcaattgtc tcattgtaat ctcttacata ttgagaaata attaaggatt acagccttta
    16621 ctttatcatg aaataaaagg gaaagataat tttatgccaa agtttttatc tattatacgt
    16681 atacgcaaaa aagctaaaac attttttgtc tttgccccta caaaaatgtt tcagcttttt
    16741 tcgtttttta ttcctgtatt tcttattttt cgaatgacag gatgattttc ccagcaggag
    16801 gatgtccttc aactagctga tgtgcttgga tgacatgctt aagtgaagca gacagattca
    16861 ttgaaataaa aacatgaaag gctccttttt ggtaggcttt gattatgcca gcaaaggctt
    16921 ctgcatctag atattccttt cttggaacaa acgattcata aaagccttcc tttttttgcc
    16981 gcaaatctag atctgggagg tcatttaatg caacatacct gccgcctggt ttcatgatct
    17041 ggatgccggt ttctcctttg atacttcctt tcgtcgcatc gattacaagg tctgcctgat
    17101 cagcaaattg cagacctgga tttgttttat cataagcagc aaatgctgaa gctcctaatt
    17161 tttttacctt ttcctcattt ttactggatg cactggttaa gattcggatt cccttttcat
    17221 ggagaagctg gatcagactt gacccgaccg cacctgatgc tcctagtatc attacagtat
    17281 ctgtcggctg gatttccgtc aaatggttga ttagattata agccgtaatt cctggcgtga
    17341 ccattccggc tgcttcttcc caactcattt tatctggaat ttttgctact tttgctgatg
    17401 gcaacacgac ttcttctccg taagtcccgc cgactgcatg gacagctact cgatcaccta
    17461 catgaacagt tgtgacatct gaaccaatct ctgtcacaat accagcgcca tcattacctg
    17521 gtacataagg gaatttcaat gttcgtatct ctttcatttc tcctaaacgc aaggcaacat
    17581 cataaggatt cacactgaat gcttttattt ctacacgaac atgtccagaa ccgacttctc
    17641 tgggatgagc atcgatttct tcaaaaacat cttgtgcttc tccgtaaccg ttaattgcaa
    17701 atcttcgcat ggtcaccatc tccctttttc tttaagttta cgtttatgcc tttattttga
    17761 caaaaaaaat gtcttttcgt tttggcgcat gattcaaaac aaaatagttg tcttttttcg
    17821 cgtaacgtgt taaattattt atctaaaagc gtaaagatta cgatttacgc taataaaaaa
    17881 taattggagg aatttgttga tggctaaaaa atcaaagatt gccaaagcaa aaaaacagca
    17941 agaaacgatt gaacgctatg ctgctttacg ccaggaatta aaagcgcaaa aagattacga
    18001 aggcttgcgt aaacttcctt tagatgctcg tccagaacga ttgaaaaatc gtgatttaat
    18061 cgatggacgc cctcgcggat acatgcgtaa attcgggatg tcccgtataa aattccgtga
    18121 attagcacat aaaggtttga ttccaggagt aaaaaaagcc agctggtaat ccaagtctgg
    18181 ctgtaatgta aaggaggagg gcgcatggac cactccttgg atcatcaaaa aagatacgtt
    18241 cgcttcttct agcagaaatg ctggtactag aagcagacgt atcttttttt acgcacagct
    18301 ggtccttttt tatatgtaaa ggctgcatgg ttacttcgct atatttattt agtaacagga
    18361 gtcagtgctt cgatacccac actaattttc aatgctcggt caacttctgc cataatgggt
    18421 cgatccaaat ggcagacctt ttctttcaat cttgatttat cgatcgttct gatctgctcc
    18481 aacaggataa cggagtctct ttctattcct gttccagtag ctttgatccc aatgtgagtc
    18541 ggaagcttag gtttagccat ttttgccgtt atggccgcaa caatgatcgt cgggctaaaa
    18601 tggttaccta gattattttg aataaccaaa actgggcgtg ttccgccttg ctccgatccc
    18661 acgactggag aaagatcagc aaaataaata tctcctcttt ttaccatagg atcctcctta
    18721 ttcttgatat tctcttggta tccgctgtcc taatccgcaa gccacttcat agtggatcgt
    18781 acctaattga tctgctatat cctgcatagt gatttctttg tctgcatttt tgccgataag
    18841 cgtcactttt gttccgacag gaaattcttg aggcaatcgg atcatcagtt gatccataca
    18901 tacacgccca atcgtttcac agtagttccc ttctaccaat agagaaaaac cctgcatctt
    18961 tcgtggccag ccatctgcat aaccgatcgg tatcgttccg atccactctg cttcaggagt
    19021 gatatatgtt tctccatagc cgattccttc acctgctggg agttttttga cttgaatcaa
    19081 ttcagaaacc agctcaagtg ctggctgcaa tggataaact tccggtagtg cgtgcccaga
    19141 gggattaagt ccgtacattg ctactccata acgaatcata ttgccgattg tttcatcatg
    19201 ccataaagcc gttgcactat tgctgacatg tacatatcga ggaagttcgg gaagtttttt
    19261 cagcacttct ataaagcgtt ctttttgcag attccaatat gtatcatctg cctgatctgc
    19321 tgtagaaaaa tgggtaaata aaccttccca ttctaataca cgactttctt tgatgaatgc
    19381 agctgcctct tttacttctt caggtgtaca aaatccgata cgacccatcc cagtgtctgc
    19441 ttttagatga atcgaaagag gcgtctctag cttacttttc tctaaaacag cagctgctgc
    19501 ttgcaaccat tcccgtgtac ctgcagttac tgataaatca tgagcaacag ctacagtcag
    19561 tgcgtcgtaa ggaaccatat tcaagataag gatgggctga gtaaatccag cttctctcaa
    19621 ctcgactcct tcatctaaat ttgaaacaca aaagcccgaa gctccacctg caactgctgc
    19681 ctcagctgtt tcaatagctc catgaccgta tccgttcgct tttactactg caaacaattc
    19741 ttttccttgc ggaagtcttg caacttcatt tgctacattt tcagttatag cttttttatg
    19801 tatcactgct ttcgttgggc ggtgccaagc gacaaccatc gtttttcctt ctttctagtc
    19861 ttccaaaata atttgtgcaa aagctgtttc atttgtatga gtaatactga cccatacatt
    19921 gccttcatga ggtgaacgcg tcacaacagg ctgaccattt tcatttttta aaatctcgac
    19981 atcttgaaag gtaacttttc cgatcccagt tccccaagct ttagagaaag cctctttgca
    20041 agcgtaacgc ccgcctaaaa attctacttg gcgatgaaaa ggcaaggact ggaatagttt
    20101 catttcatca gaggtcaaga ttcgtgcaat gaattttggt ttttcttcga tcagccgagt
    20161 gatcctagga agttcgactg catcgatccc aattcctttt atcatatgct tttctctttt
    20221 caataaattt agaacactct cattctacca aattataacc attttgccac aaataagtcc
    20281 acaaataaca aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt atgtttaatc
    20341 gttatttgaa cggattacaa atccgcgttt tttatcattg tgacgtttac cgccatcttt
    20401 ttggctgtta ttatttttgc tataaccgcc ttttgatttg tttccacgat aattaccgcc
    20461 gtcacgacga ttacggttgt ttccgccacc acgtttgcca tttttattgt atcctttttt
    20521 ctgcattggc aatgggcgtt ctggtgtgat cttcactggc actgcatcag ctggatcttt
    20581 tgctgtcgtt tttagcaaaa gtgcaactaa atcttgtgca gaatattctt ctaataattt
    20641 atctgctgtt tgcaggtatt tatctagacc attttctgca agttttgttt cgatttgttc
    20701 aactgcagca cctaattgac ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt
    20761 catccgtttt ttcgtcaagt tttcaatcac atgcaagtag tccatttcat ttggtgtaac
    20821 gaaagtcaca gacataccgc cttttccggc acggcctgta cgtccgatac ggtgaacgta
    20881 gctttctgga tcttgaggga tatcatagtt gtacacatga gtgacacctg aaatatctaa
    20941 tccacgtgca gcaacgtctg tcgctacaag gatatctaaa tgaccgctct taaatgaacg
    21001 caatacgctc attcgttttt gttgagacaa atcgccatgg attccttctg cacggtaacc
    21061 gcgtgcttct aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat
    21121 tgttaattca ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt tttcgtattc
    21181 ttttgcgcgg acatagtatt gatcgatcaa gtcagctgtc atttctttcg ctttgatctt
    21241 cacatgatgt ggatttttca tgaatttaac gccaatattc ttgatagctg gcggcatcgt
    21301 tgctgagaaa agtaatgttt gtctttgatc aggaacttgt gaaatgattt tttcgatatc
    21361 ttccaagaag cccatattca acatttcatc tgcttcatcc aaaactaatg tttgaacggt
    21421 tccaagtttc aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg tacccacaac
    21481 gatatgtgga cgatctttca agccacggat ctgacggcca atatctgctc cgccgtaaac
    21541 ggcttgtaca cgtacttttt tgtcgcgccc taaacgatat aattcttctt gtgtttggat
    21601 agctaattca cgtgttggtg cgataactaa accttgtaat tcgtgacgat ctggatcgat
    21661 tttttctaac attggtaatc caaatgcagc tgttttccct gtacctgtct gtgcttgtcc
    21721 aataacgtct tttccagcta atgctagtgg aatcgtttct gcttggatag gcgttgcttc
    21781 ttcaaaacca gcgcgttcga ctgatttcaa caattctggt gataattcta gttctttaaa
    21841 tttcaaatga atcctcctat atggttgtta actgaggatg tagctttttt gctaccccgt
    21901 cgatttgttc ggtcaaagtg tattgtttct ttttgacagt ggccacgcac gtttttatcc
    21961 attcttagcc cctgtggcac caccgaaaaa tcactttgcg aagtttaggc attagaaaag
    22021 ccaaacgggt cgcgttcagc tttatcatta ttatgacatc tggcttcaac aagttgtttc
    22081 tatggcaaaa ccagtccaat ttctcttcat ctttcggcaa taggttattc taacatagtt
    22141 tttcgttttc tgcaagttta cagattttta tttcatttgc tgaagttgtt ctaccacttc
    22201 aaataagccc atgctgttgc tgccttttag cataatacta tctgttgctt caagattttg
    22261 tttgatagca tcaatcaatt tattcttttc tttttcaaaa tagaacacag gtagttgggg
    22321 gtatttttgg ttcaattctt cttttagtgc atgcatttgt tccccataaa ggaaaatcat
    22381 ggcgaatttc tcaggatcga tatgttcact catttgcgca tgcatattta aagactcttt
    22441 ccctaattcc agcatatctg ctaacacagc gattctgcgt ccttttgttg gcagattttt
    22501 aaaagtatct aataccagtc ccatggctgt tggatttgca ttgtagacat cgctcaaaat
    22561 agctgcacca tttcctgccg ttagccattc ggttctgttt tgagtgatag aaacatgggc
    22621 gagcccttgg cgtatctcag gtatggacag tccaaagaag cgcccgatcg tatacgcaat
    22681 caaagcattc gttacattat aacttccagg cagcgggatc gtaaatgttt ctccttctac
    22741 tacaaaatcg gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat cgccatttcc
    22801 aataccgaat gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc
    22861 atctgcgggc acaacgagta atccgtctga tacaagtccg tcagtgattt ccattttggc
    22921 tttagcgatt ccttctcgcg acccaagatt ttctatgtgt gcttcaccga tcattgtgat
    22981 tgctgcagct tttggttgag ccataagtga aagttccgtg atttcattgg cgtgatccat
    23041 gcccatttcc aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag
    23101 accaatatta ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt cagacaagac
    23161 agacgcggtt agatctttcg tggtggtttt accattgctt cctgtaattg caacgacttc
    23221 tgcgcctact ttttccaagt aataggttgc caattgctgc atggctttca aaggatctgc
    23281 tacaggaata actggaaaat tttttggagc ttgttcaaca ggcaagctcc aaaatgaagc
    23341 agatgctccg tttgtgatcg cttgctggat aaattcatgt ccatctcgcg taccagcaag
    23401 aggaacgaat aaacttcctt cggttatctt ccggctgtca aattcgacat tggtaatctt
    23461 tgtcagtgca gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg ttagattcat
    23521 tgagatctct cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat
    23581 gactctcact tccaagtaaa tttctctatt cgtatttaga agctagaatg aacgcggtta
    23641 cgcaatcact ccagcctctt ctttatttct ctttattcat ttttttcaaa aaaagattga
    23701 cgctgatggt atcgattcat tcctaactgg atcagttctt caatcaaatc accgtatttc
    23761 aagcccatat tttcccataa gagtgggtac atactgaact ccgtaaatcc tggcatagag
    23821 tttaattcat tcaggaataa ttcattttta tttgtcaaaa agaaatcgca ccggctcaat
    23881 ccgcttccac ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt ttgataaact
    23941 tcttctggca cttcggctgg aatctgcatt tcgattttat tattgatata ttttgcttca
    24001 taatcataga atgctacgtc ttttacgact tcaccaggca aagtcgtccg aacgtcttca
    24061 tttcctaata cagcaacttc gatttcgcgc gcttcaattc cttgttcaac gattgctcga
    24121 gaatcatact gataggctgt tgctaaagca ttttgcagct cttctcggtt ttctgctttt
    24181 gtaatgccga cactagaacc catattcgcc ggtttgacaa acatcggata aagcaaagaa
    24241 ccttcacatt gatcaaatac ttttttagga ttttctttcc attgattctt aagtactggt
    24301 acataaggaa cttgcggcac accagcagct tgtaaaatat acttggtcat gattttatcc
    24361 atggcacatg cactggtcaa tacgcctgcg ccgacataag gcatattcaa tgtctctaag
    24421 aagccttgga tcgttccatc ttccccgttt ggcccatgta aaactggaaa aacgatggct
    24481 ccttcttctt tgatttcgcc cggattgatc acttttcctg taaagccttc ctctgtctgt
    24541 ccacttgggt cccatgaaag atgcaagaca tctttgctgg caggtttttc tgttagtaat
    24601 ggacctttga cccattgtcc ttcttttgta ataaatacga gttgaacttg gtaataatta
    24661 taataaatgg catttaaaac tgaaaatgcg gaaagaatgg acacttcatg ctctgcgctg
    24721 cgtccgccat atagtaaagt aatcttcaaa agattgtcct cctgtattct aaatcgttca
    24781 gtgatttact ctcatatttt agcacaaaac aaaaataaac gaataactta tttataaaaa
    24841 aacgagccag cttctaaaag aacgagcgtc atttattttt tgtcagctag attatttgtc
    24901 ccgtttttta taaaaagtta tgataaagaa aaggagggat gatgatggcg caaacaaatc
    24961 cacgcagccg cttggcagaa atcattcgag tgttcatcaa ttacaatgta gtgccaaact
    25021 ttgtacagca aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta ggccccacct
    25081 tcatcaagat aggacagatg ctttctgttc gagaagattt gctttcttct gccttcactc
    25141 aaacattcaa aacattacaa gatagtgtac ctagcgacac attttcaacg gtcaaaaaga
    25201 cgatcgaaac agaactttct ctttcattat cagatatatt cgatgatttt tcaaaatcgc
    25261 cttttgcctc tgcttctatg gggcaggctc atcgtgccaa gctgaagaac ggtgacgcgg
    25321 tagtcgttaa gatccaacac cctaacatcg ccgaagagat ccgacttgat cttcaactat
    25381 ttgaacgagc gatccctttg atcaaataca ttcctgaaac tagcgtagtt gatctaaaag
    25441 gggtactcca agaagtaaaa cgttccttga tcaatgaaat ggacttctta aaagaaagtc
    25501 aaaatggcga acaattctat caaaagaaca atggctggaa agagatccgc agccccaaga
    25561 tttatgacgc tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact
    25621 tgaatcattt aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca
    25681 agcagctgaa acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa caggtgtttg
    25741 atgatggttt tttccatgct gatcctcatc cagggaatct gctattccat gtactaacaa
    25801 aagaagaaca gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa gagttcggtt
    25861 cctttgcttt tcgagcctcc acttctgcag aagaccctgt tgcgccatat accatcaact
    25921 acatcgattt tggtatgatg gggcatcttt ctgcaggact taggcaaaaa ctcacacaag
    25981 ccgttttagc gctgtatacg aaagatgctt atcgaatcga aaaagcggtc cttcgacttt
    26041 gtcagcaaga aggctcgttt gatgaaagtc ggttccacca agaactgaca agttttcttg
    26101 aacagtatta tgattctcct attgatgaaa tcaacttgca agaagtattc acacatgtcg
    26161 tgacgatttg tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag
    26221 catttgggac attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc ctcatggaag
    26281 tcgcttcgcc ttttgctcaa cattattttc tcactcatct ggatgtagaa gatacattga
    26341 aacaaagcgg attagactta ttggaaggga tgaaggctgc gcctaagatc cctcaacagc
    26401 tccaccatct attagaaatg tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa
    26461 agcaagacaa attattgagc aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga
    26521 ttttagctgc attgatcgta gggtcctccc tgcttgtcca agcagcacct gtagaaaacg
    26581 cagaggtcgt ttccttatta gggatattta cgtatgctat tgccgcattc gtcattattt
    26641 ttttggcaat cgatgcattg atccaaatgt ataagaagag gaaaaagtag aaaaaggttg
    26701 tgacagaaag aatcagcttc aagaaataag aaggggtttt acataaattg tttctcaaat
    26761 ttttgtgaaa attcggctta tttttcgaag aggctacttc tgaaacaccg agaataaaaa
    26821 atgtgaaaaa gctgttctgc acccagtaat aagaacaatc aaaaagggaa catggaacac
    26881 atttatcctg tttccctgtt ccctttttga tgtattcgag cataaataaa ataccttcgt
    26941 tacctactat cttctgtact tggtaaacct tagtcaattg ccttttttaa cgcttgacga
    27001 aaagacatat gatctttctc cgtaaagggt tttggaccct ttgtccttat gccagctttt
    27061 cttaactttc cactatgata acgttgttct agaagcgtgc cgatattcgc ttcgtcaaat
    27121 tcttttccag agtgatggaa cactcgcaca cccttcggca atacaagtga agctaaacca
    27181 taatcttgcg tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt ataatccgca
    27241 ctatcagctc cgcggtctac gtagacgatt tggacatgat tagcgtattc tttggtcgta
    27301 taatggtcca cactggtcac gataaccacg tcaagaccat aggattctcc taattttatg
    27361 acatcttctt ttactggaga accatctcca tcaatgaata gcctcattct ttttcgcctt
    27421 cttccataaa tgataaacag ttactaatcc gctcagtaaa agaccaacat tgatccattt
    27481 tgccaagata agcagttgta cactttctat cggcactgat tcatggttca gcgtgatcaa
    27541 taatagatgg attggctctc ccatttcttt tgctaaaaca aacaaaagcg tcatcaataa
    27601 aaactggtgg atcacactcc ctttattttg agtagtatac ttgacaacga tccggcgatc
    27661 cattggaata gctgtatcag tttgataata ttccttattt agccagctgc ctgttactac
    27721 gtgccagaaa ctaagcagaa agacacctat cgcaaaacca tttggaatca agaggaccaa
    27781 taaagaagaa ctcagtccaa gcaattgcat agccaatgga gaccatgtta aacgtttcaa
    27841 aatccatggt ccgactattt ttgctgcaat caagcctgct gcataaggca aatacataaa
    27901 aatcgaaagt ttttctaatc cgtacagtat gccaacataa aaactgctaa ataaaaaaag
    27961 gaaattccct agcattccat ttagaaaagt aaacagattt agccagcttg gcaacttcca
    28021 ttccttcttc tggtgtccta aaacaaacac gaacaagatg aacagcagca agaaaccata
    28081 aatagcaaga tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca aaagaaacaa
    28141 taagccaaag aatatcaaaa ataaaacaag ctctcgcgta gctaccagat ttcttttgat
    28201 gtctttgaaa tccagttcgt aacgcggata atgactaacc gtatggtagg ccatgacgta
    28261 aaaaagtgta taaatagtaa agcttgctgc aatctgagtc ggtgcaggca actcaatcgc
    28321 ttgatacagg agaaatagcc agatagctgc aaataagtac tttttcccat ccatattgat
    28381 aaaaccttgc gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa gccatgctgc
    28441 actcagccct agcaaaacac ttgaaaaata aaataaagga aaataaaata cgcctaatat
    28501 tccaagcaat gaaccagcac caccaatcag cagagcgatc atcagcaacg tgtaactatc
    28561 tagaccaaat cgtagactgt tgactaaaaa tagtccagtc atccgaaagg cataaaacag
    28621 cgtaaatggc aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa ataaaagata
    28681 aggaataaaa ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa aattacgccc
    28741 aaatcgtttg agtttttcga acatgacaat ggctccttaa acagtcttat aactaatctg
    28801 ctcttcgatt ttagcattaa aggggaaata aaccaatttt acagaatata ttcttgtaaa
    28861 aaaaaataca gaaagaagca taagcttctt tctgcatttt tacggtactt gattactcaa
    28921 gcagacaagc ccttctccac aaaagtcttg cccgacaccc ctgcatccgg caggtgataa
    28981 agatagaaat caattatcct gtgcctccaa aatcaattag gagctctgta aaccagagtc
    29041 cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg cgagagcttt
    29101 tttcatttta taatcgacca tttgaaatcc ttgaccgata tctgaaataa gttcttctgt
    29161 aatctcaaat ccttcatgct tataaacctc gattgcttga tgattcccct gattcactcg
    29221 caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc gaaaaactga
    29281 agaagtcaat ccttgaccac gtactttatt tagcaaatat aatttactta aataaatttt
    29341 tccatttatc tcttcataag cagtgtaacc gacagtcttt tcttcgcttt ttaataaaaa
    29401 gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat aagttttcag
    29461 catgtaatcc acttgttttt gaccaatgat cggcacaaaa acttccggcc atacttcttg
    29521 tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt ccatacaccc
    29581 acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga gcatgatcat
    29641 gctatacaca cccaccgacc acggtttcaa gaaaatcaca atctgcataa attccctaaa
    29701 agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa agggaagcag
    29761 caaggtaaca actagtactt ttttaatatc tttcgttttt cttgtcagca attcatcaaa
    29821 tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat agtaacggac
    29881 aagcaaaaat ccgattgctt ccccaatcac taggccaata caactatata aaagcccaaa
    29941 tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca aaataggaat
    30001 gatcacttgg atcacttgca acagaacaaa tatcatcact gcatacttgc caaattgctg
    30061 gataaacgct tgcaaagacg tgctcgatcg aaaaatccca tgatgataag cataaagaat
    30121 caacacaaag aaaaaaaaca gaccaataat gggcatgatc tgaagacatt tttgtaaaac
    30181 tgatcctttt ttagacatct atattcccca catctttttt catttattaa aactgcctcc
    30241 tt
//
LOCUS       DAFGFZ010000023        29443 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000023 DAFGFZ010000000
VERSION     DAFGFZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.023"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            29..250
                     /locus_tag="LR726_000868"
     CDS             29..250
                     /locus_tag="LR726_000868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215976.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            377..1153
                     /locus_tag="LR726_000869"
     CDS             377..1153
                     /locus_tag="LR726_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL6215977.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            1156..1344
                     /locus_tag="LR726_000870"
     CDS             1156..1344
                     /locus_tag="LR726_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6215978.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            1346..1630
                     /locus_tag="LR726_000871"
     CDS             1346..1630
                     /locus_tag="LR726_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215979.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            1651..1839
                     /locus_tag="LR726_000872"
     CDS             1651..1839
                     /locus_tag="LR726_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215980.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2165..2404
                     /locus_tag="LR726_000873"
     CDS             2165..2404
                     /locus_tag="LR726_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215981.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            2410..3096
                     /locus_tag="LR726_000874"
     CDS             2410..3096
                     /locus_tag="LR726_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215982.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            3099..3929
                     /locus_tag="LR726_000875"
     CDS             3099..3929
                     /locus_tag="LR726_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBL6215983.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            3946..4797
                     /locus_tag="LR726_000876"
     CDS             3946..4797
                     /locus_tag="LR726_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6215984.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            4794..5153
                     /locus_tag="LR726_000877"
     CDS             4794..5153
                     /locus_tag="LR726_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL6215985.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            5168..5329
                     /locus_tag="LR726_000878"
     CDS             5168..5329
                     /locus_tag="LR726_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL6215986.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5326..5631
                     /locus_tag="LR726_000879"
     CDS             5326..5631
                     /locus_tag="LR726_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215987.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            5631..5987
                     /locus_tag="LR726_000880"
     CDS             5631..5987
                     /locus_tag="LR726_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBL6215988.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            5974..6192
                     /locus_tag="LR726_000881"
     CDS             5974..6192
                     /locus_tag="LR726_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215989.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            6213..6302
                     /locus_tag="LR726_000882"
                     /pseudo
     CDS             6213..6302
                     /locus_tag="LR726_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            6377..6844
                     /locus_tag="LR726_000883"
     CDS             6377..6844
                     /locus_tag="LR726_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBL6215990.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            7119..7355
                     /locus_tag="LR726_000884"
     CDS             7119..7355
                     /locus_tag="LR726_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215991.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            7379..7720
                     /locus_tag="LR726_000885"
     CDS             7379..7720
                     /locus_tag="LR726_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215992.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(7713..7904)
                     /locus_tag="LR726_000886"
     CDS             complement(7713..7904)
                     /locus_tag="LR726_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBL6215993.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            8114..8761
                     /locus_tag="LR726_000887"
     CDS             8114..8761
                     /locus_tag="LR726_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215994.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            9021..9365
                     /locus_tag="LR726_000888"
     CDS             9021..9365
                     /locus_tag="LR726_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL6215995.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9370..9651
                     /locus_tag="LR726_000889"
     CDS             9370..9651
                     /locus_tag="LR726_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6215996.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            9754..10068
                     /locus_tag="LR726_000890"
     CDS             9754..10068
                     /locus_tag="LR726_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL6215997.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10046..11740
                     /locus_tag="LR726_000891"
     CDS             10046..11740
                     /locus_tag="LR726_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL6215998.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            11760..12938
                     /locus_tag="LR726_000892"
     CDS             11760..12938
                     /locus_tag="LR726_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL6215999.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            12901..13587
                     /locus_tag="LR726_000893"
     CDS             12901..13587
                     /locus_tag="LR726_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL6216000.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            13587..14747
                     /locus_tag="LR726_000894"
     CDS             13587..14747
                     /locus_tag="LR726_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL6216001.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            14757..15632
                     /locus_tag="LR726_000895"
     CDS             14757..15632
                     /locus_tag="LR726_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216002.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            15629..15940
                     /locus_tag="LR726_000896"
     CDS             15629..15940
                     /locus_tag="LR726_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216003.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            15930..16283
                     /locus_tag="LR726_000897"
     CDS             15930..16283
                     /locus_tag="LR726_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL6216004.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16273..16674
                     /locus_tag="LR726_000898"
     CDS             16273..16674
                     /locus_tag="LR726_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216005.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            16667..17071
                     /locus_tag="LR726_000899"
     CDS             16667..17071
                     /locus_tag="LR726_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216006.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17083..17688
                     /locus_tag="LR726_000900"
     CDS             17083..17688
                     /locus_tag="LR726_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL6216007.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            17708..18070
                     /locus_tag="LR726_000901"
     CDS             17708..18070
                     /locus_tag="LR726_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216008.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18073..18255
                     /locus_tag="LR726_000902"
     CDS             18073..18255
                     /locus_tag="LR726_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216009.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18272..21691
                     /locus_tag="LR726_000903"
     CDS             18272..21691
                     /locus_tag="LR726_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL6216010.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            21742..22479
                     /locus_tag="LR726_000904"
     CDS             21742..22479
                     /locus_tag="LR726_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL6216011.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            22489..24780
                     /locus_tag="LR726_000905"
     CDS             22489..24780
                     /locus_tag="LR726_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL6216012.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            24804..26894
                     /locus_tag="LR726_000906"
     CDS             24804..26894
                     /locus_tag="LR726_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216013.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            26911..27060
                     /locus_tag="LR726_000907"
     CDS             26911..27060
                     /locus_tag="LR726_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216014.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            27057..27503
                     /locus_tag="LR726_000908"
     CDS             27057..27503
                     /locus_tag="LR726_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216015.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            27505..27642
                     /locus_tag="LR726_000909"
     CDS             27505..27642
                     /locus_tag="LR726_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL6216016.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            27680..27973
                     /locus_tag="LR726_000910"
     CDS             27680..27973
                     /locus_tag="LR726_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216017.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            27970..28194
                     /locus_tag="LR726_000911"
     CDS             27970..28194
                     /locus_tag="LR726_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL6216018.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            28191..29216
                     /locus_tag="LR726_000912"
     CDS             28191..29216
                     /locus_tag="LR726_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL6216019.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        10653 a         4436 c         6119 g         8235 t
ORIGIN      
        1 acatatttaa agaaaaggag gaaacatcat gaaacaaaat aaaaaaactc cttccgcaaa
       61 aggagcttta ataactttaa attttaaaga ccaaaagttt agtgctgttg ttgatgaaat
      121 tcatccacaa gagcatcaaa aaaattcttt tgcaactgac gagaaaaaga ctcagaaacg
      181 ctctgagaaa gttcttcgaa taattcaatt tctgttggaa aagattcgtc attttggaag
      241 tctattttga attctttatt aacagtcttg aatacggttt gtttgaataa ggcgtcgaca
      301 tccatattaa attcttccat actattttca cctcgctttc tattcaaaat tatatcaaag
      361 aaaggaatga gcaaaaatga acacaccgca aatttttaac tttgaacaac atgaagtaag
      421 aacagtaaca atacatgatg aaccattttt tgttggaaaa gatgtggcta aagttctagg
      481 gtaccaaaat ggtagtagag atattaacag acacgtcgat gtagaagatc ggcaaaacta
      541 ccaaaacggt acttttgaat cgcctagagg gttaactatt attaacgaat caggtttgta
      601 ctccttaatt ttaggaagca aacaaccaaa tgctaagaaa tttaaacgtt gggtgactag
      661 cgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa aagtagaaga
      721 agctttgctt aatccagata caatcatcca attagcaact aagttgaaag aagaacgtac
      781 tggaagacta atcgcagaac aaaaaattgc agaatacgaa cccaaaattt cgtatttgga
      841 tagtatctta tcttctacag attcagtaac tattagtcaa atcgcggctg attatgggat
      901 gtctccacaa cagatgaaca aactacttca taagctaggt attcagaaaa aagtaggcaa
      961 ccaatggtta ttatgcaaaa aacacatgag acagggttac acaaaatctc atacaactga
     1021 gattccgaaa tctgatggcg gaacaaaagt tgtgatgaat acaaaatgga ctcaaaaagg
     1081 gcgtctgttc atttatgagt cgctgaaaaa agaaggatat attccagaaa tcgatttatt
     1141 agaggaggaa tgacgatgtc acaagaattt attttgaaag tcagaattca attagcaaaa
     1201 tacggaaagt ctcagaactg gttagctgat actatcggaa tttctagacc atatatgtcg
     1261 gacattatga atgggcgtag aaaaccagat aaacaaatca agccaattga ggcagcacta
     1321 gcagagttag agaaggagaa ataacatgca aataacaatt ccagataatt tagtagtttc
     1381 cgaattaact acacagatta cgaatgctgt tcttaattca ttggacgaac gattacacct
     1441 tatgaacaaa tcagtagagc ttcctccata tccaaacaaa tcagaggtaa aaaaagtttt
     1501 aggcattggt gatgacaaat taacacattg gataagccta ggcttaaaaa cacagcagtg
     1561 gagcaagtta gacatcagaa ttgaacgatc ggaactccaa agatttttga aagaaaactt
     1621 tgagttctaa aggcaaagga gaatgatttt atgtcctaca cattgcaaca agaacatcaa
     1681 attctaggtc tgattaaaca gcgtagaaaa caattacaag atgatcgtgc agcgcttaga
     1741 aaatccgatg agctatcaga tagacaagct gaactaattg cttctgaact tgaggatttg
     1801 agaatgctag aaataaaaaa tagggagatt agattatgaa gaagacagac acacttttta
     1861 taggattcat tttggggtta ttagtgattg tagcgcacca aagtattatc ggaggaagct
     1921 tgttcgcagc attgatggtt ttaatcaatc tgcttgactc aaaagaaagg agcaactatg
     1981 gcacgagaag aagcgctaaa aatcggtaaa gtgattgctg ataattggtg ggcaaatagc
     2041 cgttctatta ttttaagcaa gcaacatatc gaaaagaaaa aagcatggca acaaataaaa
     2101 aagtgactcc gccgaccaaa gcaatgagtc actcaaaaaa tcataactaa ggagatttta
     2161 acatatggaa aatgaacttt ccactctaga tcaatatttg gccgatcctg attggggcaa
     2221 atcgaattta caactggttg ctaacaagta cgaaaaatca attgatgaag caattgttgc
     2281 attgactgat gcagcttcca taaccgctga tgacagtaaa tggaccaaga gagacttggg
     2341 cattctaaaa cgaggcgtta attggcttga agatcagtac agagaagaaa caaaagagaa
     2401 gtgatatgag tgtttaaacc actaatagat tcatactcgg cggtactaaa gaaatttaaa
     2461 ggtaacgaca ttggtgcaac aatcaacgaa gaagtaaata tcgaacggct gaaaacgatg
     2521 tatgacggat atgatggcga tcgagtcatt gaaattcgct ttattgatcc acgacggttc
     2581 acagtacagc aaagaaactt catctatgcg ctcataggcg atattttcat cgatacaggc
     2641 atgccaacgg acttctggaa ggaattcttc tacttccgtt ttgaaggtgt cacagggcgc
     2701 gaaataagcc tcaaagacga atcgaataca accgtgagtg atgccaatat cttagcgaat
     2761 atcatcctag atttcatctt tgaacatcat attcctttca aagaaggata tgagatttta
     2821 ccagcgaatc aagagtatta cttctacaaa tgcattacaa aaagagtctg ctgcatctgt
     2881 ggcaaaacag gagctgatat cgatcacttt gacaaagcgc tgggaagacg aaagcgcaaa
     2941 gaagttgatc attcagagta cacatttgca gcactctgca gaatccatca cacagagaaa
     3001 cacaaaatag gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat caagttaaac
     3061 caggaaacaa ttaaaaagtt aaggatagga ggataaattt ggctgagata agttggatca
     3121 aacttaaaac tactatgttt gacgatgaaa aaatacgatt aatccaagct gttcctgagt
     3181 cggatgccat catcgttata tggattcgat tactagtttt agcaggaaag actaacgacg
     3241 atggtctgat atatatccag aggaacatgc cttataccga agaaatgctt gctacattgt
     3301 ttggcaaaaa cgtaaatacg gttcgcttag cgctaactac attggcaaat ttcaacatga
     3361 ttgatctaag cagtgatgga ctaattgcca tcagtaattg ggaaaaacat caaaatatcg
     3421 agggtatgga taaagtaagg ctaaaaaatg ctgaaagaaa ccgtaaatac agggaaagaa
     3481 agagacagga acgtctcaaa ttggaaaatg acgttagcgt gacgtcacgt gacggtacag
     3541 ataaagatat agaagaagat aaagatatag ataaagaaga aaagaaaggt aagtattctg
     3601 acgaacactt acgccttgct aaaaagttgc aaagtaattt aactgaagat tttccaaaag
     3661 aaatgaacaa agtagatatc gaaaaatggg cagacacaat caggttgatg gaagaaagag
     3721 ataaagcatc tatagaagcg attgagtatg tgatcaattg gctacctaca aatgaatttt
     3781 ggtttggaaa tattagaagt gctaagaaat tgagagaaaa atttgagaag ctcaaattcg
     3841 aaatcaaagc agacaagaag aatcataaaa agcaaagtca aaaactacag tacagcaatc
     3901 ctagtgaata tgacgacttg ccaatttaaa aaggagatgc atcacatgga aagcctagca
     3961 aatgctatgg agaaactaat aagaagagta ttagtgcaaa gcggaaaatg tccagaatgt
     4021 agcgaacctt tgtatagttg gcgagctaaa aataaggatg gttcagaacg ttgtaaacca
     4081 acatgcatga gttgtggtta taaagcgtta cgtgtgaaag aggatataca gaccgaacgg
     4141 atatataacg atagcttaaa agcacgagcg ttgagttttt ttcaaaatgg ttcggtatta
     4201 acagataaaa ctttgtttaa atgcaaaatg gaaaattatc acgtagtgga tcaagaaaca
     4261 aaaatcgctt tagaaaaagc aaaaagctat acgaatgagg ttctgctgaa ccatcctgca
     4321 catttcattc tatcagggaa atcaggaagc ggaaaaagcc acttatcaat ggccacagct
     4381 tgggaaatac ttgagcgctc aaattatgac aagaaaatac tttttataag ctatcaagaa
     4441 ttattagagc aaataaagtt ttcttataac aatactgaac tgagaaaaga aattgaagga
     4501 tcgcttatag ccgatattaa aacaactgat ttggtggttt ttgacgatat tggagctgaa
     4561 ttaggtagtg gggtatcaaa tagtaggcag tttacaaaca acacgttaaa cacgctctta
     4621 gaagccagac agaacaaggc aacgatcatc acaacaaact tatctggtcc tgaactaaga
     4681 gaagcctacg gtgaaagaat tgtttctagg atatttaaga attcagaagg ttatgcgctg
     4741 aaattccaac aaacagcaga caagcgcata aaaccagtga aaggtagtat cgcatgaata
     4801 aataccgtaa taaaaaaact gttcatcgag gtatcaagtt tgattctatc gcagaagcag
     4861 agtattacga tctagccttg tggcaagctg aagcaaatgg ctggaaagta aaacttcagg
     4921 aaagatttga gctgatgccg aaatttgaac tagacggaaa gaagtatcgc aagatcgagt
     4981 atattcccga cttcacattt tataaaaacg gcaaacttgt caaagtcgta gatgtcaaag
     5041 gaatgcagac aaaagacttt aagatcaagg caaagttgtt ctgtcatcaa tatcaagcgc
     5101 cgttgatatt agctaaaaaa tatcggaata cgttcaagga agagcgtttt taacgaggtg
     5161 gtccatcatg acaacagaag aagtgattca aatgcgtatt cgaaacattc agcgtgaaat
     5221 tgacgatcta gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga
     5281 tttgcacata gagaacttaa gaagggaaat tcgtagattg gaggaatgag cgtggataag
     5341 aaagcgacaa tgaaacgaat tgctgaatta accaagtcag aatcttggca ggaagacaaa
     5401 gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcctaaacgg
     5461 aaaacgccga gaaaaattgc aatctggcat ggtgatcgaa ttctagtaac aggtacagct
     5521 gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag agctaggagc
     5581 ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata atggatctca
     5641 ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag aaagacaaaa
     5701 aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga gcaggagtgc
     5761 gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac gaaattcaaa
     5821 aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag aaacttcatc
     5881 aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat ttgaggagga
     5941 cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat cgaatcagga
     6001 gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt
     6061 attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca
     6121 aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag
     6181 gacagcgaat gataccgaag tttagagcgt gggatagaga aaaagacaga atgaaggcag
     6241 tagctttggt tgaatttgct gaggcgtata aatcaggaga actcatttca cgtacaaaat
     6301 aaaaaaagtc ggattcctcc gactatgaat aatatttccg acataagtat tatatcataa
     6361 ttgggggaat cagaggatgg tacttttcga tgtaaagaaa tatgagacac caagcgcgaa
     6421 agatgttgat atggaacgca caaaacataa tgtcgctgtg tttctttcag catatctatc
     6481 agctagatgt agagtagggc aacctcgaga accaaaagtg acagcatctt attccttggt
     6541 tccaccttct acagctgatc atatatttga agcagaaaga atgctgatcg ataaagaaga
     6601 agcacaggaa gaatttgagt atctgcataa attgtttgtt cgaggctatt cagcgataca
     6661 gcatccgcat aagcctgatg tgactgaaag gcgcaagaag atattctatg atcgttatat
     6721 caatggtctg cccatttatg taactgctca aaggaataat actagcgaag aatcggttaa
     6781 agtagaatca aacagaatta tcatccaatt tgcttcatcg ttagaactgg ttgctttcaa
     6841 gtagccagtt tttacacttt ttataccctt ttattaccaa tttggtttcc attttatacc
     6901 ttttttatac caatcactta cctattcaac gttgtattat gatagtgtcg aaagattagg
     6961 aaacaggatc gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt taattaagct
     7021 tcgataggca gcagcggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt
     7081 gttcttatta tctatcatcc gttgctgtct attaatttat gtattggagg gtatataaaa
     7141 tgaagatcgc ttccgaaaaa attgatcaac taacaatttt agtaaaccaa agaatcgaag
     7201 atattatcaa agaattcgaa ggtgaaaaga ttaagaacat aaagcttact ttaatggatc
     7261 ataagttcga attaaattcg acaaaaagga agatcgttga gcttatagaa gaagaaatag
     7321 ctctccagat caatgagcaa caattagcta attaatttat gtattggagg ggaaatgaat
     7381 ggaagtcgaa caaataaagt atcaaggtat ggatcgggaa cggttgattg atattattga
     7441 gcaacaacgt ttagaattga tccaaaaaga agctgtatgt aaagagtaca aaaaacattt
     7501 ggaacaagta attgagtatc attcagtaga aaagtacaga tcagtggtgc aaaaaaatag
     7561 agcaacgagt cctgattctc gtcaagatgt atcgattagt atcaagacgc cagtatttaa
     7621 ttctgttaaa tcagaaaagc tagaaagtat ctcaactgct ttggtagaaa aagcagctga
     7681 aatgaactcc ctagcaaatg aagttagtcg tcttatttag tcatatcgat aacttgagag
     7741 tctttggtga ttccgttttt tatagaatta atggtttgtt cagcggaata tttagtcaag
     7801 tatgtttcgc tagttgctac tacttcattg ttattggact taataacgaa gtaatattgg
     7861 ccattagttg cttctcttat aacaaagtac aaatttttca ccacctataa tttatttcag
     7921 cggaccactc gctgataatt aaaattataa gctatgtata aaatttcaca atatcaattt
     7981 gtcgctgtgg cggaaagggt agacgcaata aaggatgaat gggaaaagtc aatgagtaat
     8041 tatctgaaat ttacaagtcc cctgtttagt tatctaagat tatttgataa tattatttct
     8101 gaggtgatag aaaatggtgg gcgtagttaa atttcttggg tttacatttg atggatatgg
     8161 tacgttaggc gaatgggtag gtattatttt gatagtgatt acaattcaac aaacatcaaa
     8221 gtactactgg tattctaata gttctaaaat tgtatcaaga ataactgaag gaataaccaa
     8281 aaaagatatt actgtcgtcg gtaaatatgg gtttactata actttattaa atgaagggaa
     8341 atctgaaacc gcaataaagt tttgcgggat tataggtaaa tataacattt tccaacgttt
     8401 tatcagaaag cattggtaca aatatattta caaataccct gtagttaaaa agcttattga
     8461 aaaatatgtg gaccacgaaa tatcttatct caatccaaca gaccttatta atcaagaatt
     8521 ccagaatatt ggatacttag aaaaaggtct aaaaatacca attgtaaatg aaagtttagt
     8581 tactgcaatg gtagctaaca taaagaacag caagaaatta gaaaagagat ataaaaaatg
     8641 taaaaaattg aaattttcct ttttattctt agaatataat ggcaaagaat attatcaaga
     8701 tatttatgtt ttgcgaggct cgaacttata taaagacatt aaaaataaga tttcagtatg
     8761 agatttttta aaattcgtag atcactcttt gagtggtctt tttattttgc gtaaaggagg
     8821 cacgacgatg tatagaccac aatacttaga acagaagcat gaagtaatca ctgtgcaaaa
     8881 cggtaacggt gagatagtac gaaagtatag aagaccaata aagagcgata catataaacg
     8941 gaaggaaagc aatgaagtta ttccattgta tggcaaaaga atagctaagt attaaataag
     9001 attgcgaaag gagatgggac atgaccgagg aattctatag atggttatta cagttgataa
     9061 gagaagatcg tttggttaag ttctatcagt ctcctaaatg gcgcaggctt agagagaaag
     9121 cgatgaaacg agatcactat gaatgccaag agtgtagaag actaggtaag tatcatagag
     9181 tagagaacgt tcatcatata aaggaagtca aggatagacc tgacttagct ttagatttag
     9241 ataatcttat ttgtttatgt gttgaacatc ataatgaagt tcatggcaga tatcttacag
     9301 cgttagataa acaagagaag aagatagaaa gcttcgctaa cttcgatgca agtgaaaggt
     9361 ggtaagtgca tgatcatcaa tgacaatggc agagagtatg atacagaaaa gattgaagag
     9421 tattcatctt atactcaggg attaattaaa cgtttgatat acgttcgcta tgtaggtatt
     9481 agggatctgt tatcagataa ctgttgtagt aaatacaaag tgaatcaagt aagagaagcg
     9541 ttgaataaag ataataacgt cgaaagaata aaaaatgttt ttggatatag tattgaagag
     9601 attaattatt acattgactt cgctgaagct ttcattccga tggtgagata accccccctt
     9661 aaaataaatc gcaaattttt tgggggtgat gaaacggagg gggctgtcag gaaaagagat
     9721 tttttcgaac tttatcatga aaggagggct aaaatgttta aaaacgaatt gtctcaaaat
     9781 cgctacagag aaaaattacg ccgctcttta ataagccaat tggaaagtca gaaaacaaat
     9841 attgagccat tcttagataa tgttgatcgt tatatcagtt tatgggaaac ggcgatatca
     9901 ctggaagaag atatatccga gaacggcatt agactggaga atggtaaaaa gaatgaatca
     9961 gtagcgttgc ttgtttctgt caacaaacaa atgggattga tgttggataa acttgccatt
    10021 actcctgaat tggtaggtga agcaaatgaa tcaattcctg agttataagc atattgaaaa
    10081 ttggttcaaa gctatagaag aaggcactat caaggtatgc aaagagcaat tattgctaaa
    10141 aaattatcta gaagaaagag tctttactag agaagatatt tacttcgata agcagatggt
    10201 agaggattca atcaatatac cagcacaata ctttccattc gaattaattc cgtgggaaaa
    10261 atttctacaa tgttttattt atggtgttcg atggaaaaaa gataaaacac tagtgttcaa
    10321 tagatatctt tcattaatgg gacgtggtaa tggtaaaact ggttttgctt cttggaacaa
    10381 cttctttcta ctaaccgcta aacacggtat taaaaattat gatattgata tctatgccaa
    10441 taatgaaagc caagcaaaga ctagttttga tgatgtattt aaagtaatta aagatcatcc
    10501 tgatttagat aaaaaagtat ttaaagctac gaaggaagtt attcaaaata tcgctacaaa
    10561 cagcaaactt cgttataaca cggcaaatgc tagaacaaaa gatgggaagc gaccaggtgc
    10621 aaaccgcttt gatgaaattc acgaaaatga agattattca atgataaatg tggctacttc
    10681 tggtggtggt aaaattcgag attatagaga attttatgat acaactaatg gtcatgttcg
    10741 tggtggtccg cttgatgaca ttatagaaga atcaaaaatg attctttctg gagaacttgg
    10801 aattgacaag gatggagcag aattttctag tttgtttcca tttatttgtc gcttggataa
    10861 cgataatgaa gttgatgatc ccgacatgtg ggaaaaagct tgtccaacta ttaattacaa
    10921 tgcagattta aaacggaaaa tgtttcaaga atactctcaa atgcaacgta atgctggttt
    10981 aagacttacg ttcatgacca aacgaatgaa cagacctatg gaagatacac gatttgctgt
    11041 tgcttcatat gatgatgttc tgcatacgaa agaaaaagaa tttcctgaaa aaatggatga
    11101 agtgatagga acagtcgatt ttgctgatag acgagatttt gccagcgttg ggttgctagg
    11161 aaaatacgat aaagatgttt attttacaca acatactttt atccacgaat cagcccttcg
    11221 attacaaaac atcaaacgag aggttataga tatttctata gatcaaggaa aatcacagat
    11281 cgttcatgga aaaaatatag aagctgatta tattgtaggt tggtttcttg aaatgagtaa
    11341 taaatattat attaaaaaaa tcgctatgga tatgtaccgt gcaaaaatat tgaagcccgc
    11401 tttagaagaa gcaggtttta ctgtggaaat tgttcgaagc ggatctgtta cacatggtat
    11461 gttaaaagat ctggttgatg acctttttat taatcaacgt ttattttttg gtgacgatgc
    11521 gattatgcgt tggtattgca tgaatgtata tgaagagcat atttctaatg gaaatatacg
    11581 ctatgaaaaa atagaacctg aaactagaaa aacggatggc tttttttcat tccttcatgg
    11641 tttgaatttt ttagatgata tttatgattc tgctcctgta acagtcacaa atagctcagt
    11701 agaaaataca ggaactggat ttactcctct agtattctaa cttgaaagga ggtgagaaag
    11761 tggggatttt tcaaaaggcg gtaggatact tcacaaaaaa agcaacggtt cctttagaag
    11821 aatacttttg taaattgcaa gttgattttg tgtatcgaaa atttgcgatt gaaacttgta
    11881 ttgatttgat tgcaaatgcg atgagtaaag cggaattcaa gtcatatgaa gatggaaaaa
    11941 ataaaaagaa tgatctttac tataggctga atgtagctcc taataagaaa aataatgcaa
    12001 cagaatttag aaaaaaactg atcaggagat taatattcta caatgaagta ttgatcgttt
    12061 ctccgtctaa taattctagc gaaatattta ttgcggatag ttgggatgtc acagaatatg
    12121 cattgaaaga tgatgtgttt tctcaagtgc aaattaacaa catagtcctt gatagagaat
    12181 ttctagaaag tgatgttatc tatataaaat acgcagatca acaaattagg caactagtcg
    12241 atgcgtatta tcaagcgtat gggaaactca tttctagtgc catgaatgtt tacaagcgct
    12301 ctaacgctcg tagatacgta ctgaaaggga atttattccg accgcaagac aatacaacac
    12361 aagatcaaat caataaaatg atgacatcac aatttaaggc ttttatggaa gctgataatg
    12421 caggtgcggt atttcaatta caaaatgagt acacattaga agatttcagc ggaaacttcc
    12481 aaagcaattc aagagatata aaaaacttaa tagacgacat ctttgagatg acagcagcag
    12541 cgtttcacgt tccgaaaaac ctactaaagg gagacatgag tgggttatcg gatcaagtgg
    12601 acgctttttt aatgttcgaa atcataccga ttgctgaact tattcaggat gcgtttaacg
    12661 ctagtctcta tgaagtagaa gaatacttgt cagggaattt tgtacgtgtg gatacaacta
    12721 tgatcaagat tactagcttc aaagatttgg ttgacgctat tgatgtaggc attagaaatg
    12781 gagtatttac aatcaacgaa ggaagagagc gcgttggaaa tgatcgctct gataaggcga
    12841 tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga ggtgaggcga
    12901 atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc gcaaattttt
    12961 attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa gggaatcaaa
    13021 aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa ttcaaacggt
    13081 ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa agcaaaagtt
    13141 aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat ggctggcgat
    13201 actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac atacggagaa
    13261 ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga taaatcggta
    13321 aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga acttcttgaa
    13381 aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga tgaaattatc
    13441 gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga agaaattttg
    13501 aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc attaaataaa
    13561 acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt gtaacagctg
    13621 caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt agcgcagcta
    13681 tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca gcaaaaaatc
    13741 aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa gaaacaaaat
    13801 tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta gtgccagcta
    13861 ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg caaaaaatta
    13921 cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc aatccagcat
    13981 ggtggggtaa actttgcgaa gctgttaaaa aagttttaga taatggcttt gatgtaatta
    14041 acatgaagca gttcaagcta tcaggttata ttcctgtatg taaggcaatg cttgatttag
    14101 gtccagtatg gttagatcgt tatgtccgta ctgttttagt agaatcattg agaattgcat
    14161 tagaacaagc aattgttgat ggtactggta aagatatgcc agtcggaatg atgcgtgaca
    14221 tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc agaacctatt acagctttag
    14281 atgctgtaac tatgggcggt ttgatggcgc gactatcaaa attcaatatc gaaggtgtga
    14341 atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg aatccaactg
    14401 attactggtc taaagtattc ccagctaaga ctgtactaac tgctaatgga gaatacgtac
    14461 aagtattgcc agtaccagtt tcagatttgc agtcaacggc tgtgccagaa ggaaaagcag
    14521 ttattggggt agcctcagat tacttcatgg gtgtaggatc tacactaaaa attgaagctt
    14581 cagatgaata ccattttgtt gaagacgaac gcatttatct agctaaacaa tatgcaaacg
    14641 gtcaacctaa acgtaacgat agtttcattg tattagatat tagcgctttg ggaactacta
    14701 ctacaactac aaaaccaaca accacaacaa ctacaacaca agcgtaggtg atcagaatga
    14761 agtatattct ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa gtttgtttaa
    14821 ctaatctgaa gaaactagga atcaaagata tcgtattgct tttcagcaga cacgatgatc
    14881 agattcctat tttttttgag aaggaatatg gtgttgaagt tcatgtgtac gatgatctgc
    14941 gggacgacaa agagtatatt ccttcgatta aaccatattt atggtggaaa tatttagaag
    15001 aagatcattc gcgtgaggac gaccgatatt tctatatcga ttcggatgtc attttcaata
    15061 aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt agcgactgct
    15121 gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat attctaaaag
    15181 atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac actaattcag
    15241 gaggcgcaca gtgggttatt aaccgcccta aagcgaatta ttggaaaaag gtttatctgg
    15301 attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatatacaa atctggacag
    15361 ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct aaagttcacg
    15421 aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa gtaaaaatct
    15481 tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg agatacgtga
    15541 cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc tcttacgcat
    15601 atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg aagaattgct
    15661 aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt caattaaaaa
    15721 gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac tttctttttc
    15781 tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact ggaatcgtgc
    15841 tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc aacattatgc
    15901 gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc gaagaaacat
    15961 tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa ctaggaaaaa
    16021 agattggtgt agaagataca gaaatcactt ctttaaaatt tagaaatctt tccatgagag
    16081 atagtgatat aacagctatg gatgcgatgg gatcgaaatt aactaagaaa gtaaagactc
    16141 catttcatcc aatcgccaag aaatttaata aagatcaata ttttatcgta atcgatagta
    16201 tgcgttacaa cgttatctat gccgattacg ataattttta tatctatttt tatcttgaaa
    16261 gtgtgggtga atatggtgat tgataattct aaagaaaaag aacgtttaaa taagcaaatt
    16321 tctgctatca aaacttcctt agaagagcat tttggcctca aactctttca agactccgta
    16381 ggcgaggatg agctacctga tgattttaat tacttcattc tcgaaacagg agaaatagaa
    16441 atgatcactg agccaaaata tagcgtgggt caaaatctat atctaacttt ctattcagaa
    16501 aatagagaag atttaacagg agattcacta gatattattt cattgattca aaatcgttcg
    16561 attcgttttc agagaatgga tcccaatcat ttaaaactag agaaccaaga tcgctatatc
    16621 gatcaattgg tatttacgtt tagacgatta ttgaagagtg attgtcatgg ctaaaaatag
    16681 ttgggagcta aaaataaatg gacatgatga acttcttgtg cggatggaac gctattcaag
    16741 cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa ggttcggcta ttgcagtgga
    16801 taggattaca gaaaaaattc ctgtttctga agcagattta agaagagggc accaacacgc
    16861 aaaaaatagt cgtccactta agactcaata cattaatttg ggtttcatca ttagacctac
    16921 aagaaaattt gagtatttaa aatatcctga tttggggata ggtacttcta aaagaaatca
    16981 gccggacgaa tttatgagaa gaggattagg tcttgcactt gatccaatta cagaacttct
    17041 gattcgtcaa ttcgataaat taaataaata ggaggaacaa caatggctaa aacaacaaca
    17101 gtaacaactt ttgacaatat aagtattaaa agaatttctt ttaattttaa gaatgcaaca
    17161 aatgcaattt caactgattg taatggacaa ttagatggcg aaacagaaat gcagagaatt
    17221 gtaaaaaaat gcggttcaac agaagtaaaa tcgaaatcta aaccaatcaa tatgacggta
    17281 acaattactg cacatgtacc gatggaagtt tatcgacgct tcaatgggtt gaaacaagat
    17341 gaacgtatta aatcgggaat ttactcttat ggtcctgatt ccgtagggga agatttctct
    17401 cttgctgcag agatcgtgga tgatttcgaa gaaaaaaata agctgattgg tatgttagca
    17461 tgcacttcga atacaggatt aacattctct attgaaaatg gtgcggatga agtagctgcg
    17521 ttagaactag aaacaaaagt tatgcaagat gaatttggta aattttatca tgaagcaatt
    17581 gttgcagaac ttgaagaaga cttaacagat caatggatga cgaatctatc tgctgatgtg
    17641 attaaaaaaa cttcagttgt gacaactacg gccactcaat cacagtaaaa aaaacggagg
    17701 tagcgaaatg aacgaagatt actcaaaaat tgaactaaac gatggaacaa ttttgaattt
    17761 agaacctaaa ctgaatatca agaaattatt gatgatcaat agagatttta acacagacga
    17821 gtttgcaaaa atgactgtgg gaaaaggatc catggatatt tctgttattc aaggtgcaaa
    17881 ggctgtgtat attgcttacc gccaagcgaa catgactgat tatatttcat tcgatgaatt
    17941 tatcgataaa tgggattttg atatggctac tgccagctat atttatcaat tgatgatgtt
    18001 caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg aaaaaaagct
    18061 tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg tcgatgtcta ttcgatgttg
    18121 accgacgttt tttctatgcc ttcagatttg gttttaagcg atatctgttt agatgacatt
    18181 ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga aaaactatgc aataaaccaa
    18241 tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa aagagaacag aagcagaagt
    18301 aacattcata gctaacgatg acggattgaa atctacgtta aaagaaatca gtgctgaatt
    18361 aactaaaaat agagcagaat taaaactaga acaagctcaa ttacaacaga ctggttctga
    18421 atcagacaag ttaggaagta aattatcttc tttagaaaag cagtatgaat tacaaagtca
    18481 aaaagttgaa gtaactagcc aacgtttagc caatgccaaa aaatattatg gagaaaattc
    18541 caccgaagtt cagaaacttg agagagaact gattaaccaa caaacagcgc aacaacgttt
    18601 gtcaaacgaa attgataaaa cgagtaatgc actagctcaa gcaaaaggcg aaatacagac
    18661 gtacgagtct acaatgcaac agttggatag tgaacaaaaa aatgttcaag ctagtgcttc
    18721 tctgattgaa tccgaataca aaaaatggca agcaactgct ggtcaatcag cttctgaatc
    18781 cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg cagagaaaac
    18841 gatagatatc ctgagacgac agttagaagc tacacagtct gagtttggcg ctacatccac
    18901 agaagcaatg cagatggagg cgaagcttaa tgatgctgaa cgtgaatttg aagagttagg
    18961 acaagctgct aaaaatgtag atacaactaa cttggacgat atcggaagca aaatagatat
    19021 gaataatcta atggaagctt ctgacgtttt aagcgacatt ggcgataagc ttacagaatt
    19081 agggaaacaa gcagtggact ctgctaatag tgtaggtagt tcccagagta aaatacaagc
    19141 taattttggt ttgactaaac aagaggctga agaattaacg aatgtagcca gagacattta
    19201 ttataaaggt tttggagaat cgttagatca gtccacagat gcattgattt tggtaaagcg
    19261 taatttaggc gatttaaata atcaagattt acaaaatatc acggaacaag ctatggtcct
    19321 agaaaacacc atgggcgctg atatggatga aacgttacgt ggtgtaaatg gcttaatggt
    19381 caatttcggc ttgagcgctc aagatgcaat ggatttaatg gtttcgggta ctcaaaacgg
    19441 tttagataaa acgcacgaat taggcgacaa tatggcagaa tatagccaat tatggagtca
    19501 aatgggatat tcagctgatg aaacgttcgg aatgcttcaa aatggtttag atgcgggtgc
    19561 ttataacctt gataaagtca atgacttagt taaggaaatg ggaatatcgt taacagatgg
    19621 tcgatttgag caaaacatgg atatgtttag tgaaagtact agaaaagctt ttgaagagtg
    19681 gaaaaatggc ggaggaacac aaaaagacgt tattaattcc atgattcaag attttagcaa
    19741 tatggatggt caatacgacc aattaaataa agcttctaca atttggtctg cacttggcga
    19801 agataacgcg atgaaagttg tccaatcttt gactgatgtt aaccatacat ttgatgatgt
    19861 tagtggatct gcacaaaaaa tgaatgaaga ttctactact ccgttacaag aattaaacgg
    19921 aaaaatagct gaattaaagg attcattagc tcctataggc aacacaatca tagatgcact
    19981 cgaaccagta attgattttc taggaaagat ggctgatgcg tttaataatc ttccacaacc
    20041 agtacaggat tatgccgtag cgattggcgg attgactgct gcatttactt tattaatgcc
    20101 aataatagtt ggcttcatgg ctctaggtgg tcctactaca ttaataatag gagcagttat
    20161 tactgttatt gctggagtta tagcaattat aaaaaactgg ggcgcaatta ctgactggtt
    20221 taagggaata tggagtaaat tcactgattg gttgggtggt acttgggaaa gtataaaaga
    20281 aggtgcctca tcagtttggg atggagttaa agaaacctgg tctggatttg tagattgggt
    20341 tcaagatatt tggcaaggag tttctgattg gtttggagag ctatggagcg gattagttga
    20401 aggagcttcc aacatctggc aaggagtcca agagacttgg caagcattcg ttgattgggt
    20461 ttcaaatatt tggaacggag tcaaagaagt atggtcgatt atttgggcag acattgtagg
    20521 aattgttcaa ataccatgga ccttaataac gtcattgatt caagccggta ttaatattat
    20581 cgtgggtatt tttgatgtag ctggacagtt attaggcgca gcttggcaag ctgtttggac
    20641 acctatttct gatttcctta aaaacacttg ggatactatg acacaatggg taagcatcgc
    20701 ttggaatgga attgtaacta cattccatac tatatttgat ccagtagtgg catggtggaa
    20761 tggtatatgg acagctatta gtactacggc ttcaaatatt tggaattcaa ttagtgcaac
    20821 agcttctagt atttggaaca gtatcaagaa tacaatcact agcttggtac aagcagctgc
    20881 tacagtaatt caaaatattt ggtcaactgt atctagttgg ttaggtggaa tttggaattc
    20941 aatcagctct acagcatcaa atatctggaa tagcgtgact agtagtataa gcaatgctat
    21001 aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt ggatcagcgg
    21061 aatttggaac ggtatcaaaa acactgcttt gaatctttgg aatggaatta caagcactat
    21121 tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca atctaacagg
    21181 tattgtttcc gacatattca ataatgttaa aagtacaatt gctaacattt gggaaggtat
    21241 caaaaagact gttagcgctc caattgattg gatcagagat aaaatcagta gtatctttga
    21301 taatttgaat atttctatac cacatattcc gttaccacat tttaaattga gcggagaatt
    21361 caatccattg aaggggaaaa tcccaacgtt gggtgttgat tggtatgcga aaggtagtgt
    21421 gtttaattct ccgaatatta tcggtgtcgg cgaagcagga cctgaagcag ttttaccttt
    21481 gaaaagatct gtgctgcaag aaattggtga tcgtatcttg agtagcacat cagtttcatc
    21541 tagggcacaa acgattcaac ctgtgaataa ctacgaattc aatttcacaa ttgatggtaa
    21601 cgcagatgag gttactatga agcaaacaac tcaacaaatc attgatagca ttacaaaagt
    21661 tcaaaatgat aatgcttcgg catggcgtta aacaggagag tatttctcct gtttttttag
    21721 tattaaaaag gatgtgaaaa aatgactgat tgtatacatt ctataatcga tggatttcct
    21781 gattatttgc ataaattggc tttagctgaa agaccaacca taccttctcc aaaaagacag
    21841 agagttgaaa cttctgtttt aggaaggtta ggtggcttag tacaagatta ctcgtttgaa
    21901 gacatgtcgt ttacattgca ctataactat ttagaggatg tggaagacca tcaagcgttc
    21961 aagcaatcgt tttatatcat gcgtcattgg ttaaattatg caaagaaatt agaattctct
    22021 gatgatccca acgtctatta tgttatccag actatcgata ttggggatgc agaaaacgat
    22081 attgttgaat ggggagagtt cgatgtaaat attactgcga aaccattcgc aagagttcaa
    22141 gaagatgtac ctataaccgt agataaacca cagtcattta acttgctgaa taatagttta
    22201 gaagaaagtt ttccaaagat tatcatcact ccttcagcta cttcatgcca gttcatctta
    22261 aatgattatg tgtttagttt tgaaggctta gtagtaggaa ctgacgtagt cattgatagt
    22321 gatttgatgc tttgctacga agagcaatcg gacggagata ttttagatcg gtccaacaaa
    22381 atgaagacca tgcaatatcc gacattgcaa gtggatatta attattttaa ttgtactggt
    22441 ttgagcaaaa tacaaattta tcgtaatggg ttaaggtagg tgaaatagat gatcgataat
    22501 ttaataacta tttacgataa aaacgacgcg aataatttag ctgaacattt atatgatacg
    22561 caaggtttag gcgctttgtc agactggtta acagctactg ttagcaataa attaaacgga
    22621 gccgagatat ttcagggtac ttatccaata agcggaacta atgcagattt gattgtagaa
    22681 ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct acggatttat
    22741 tatgcaaaga cttctgtaat aggcaatacg atagaagtaa aagctgaacc tattttcaat
    22801 gatataagaa aatcggtgtt gaataaatat gacagtggaa cagaaaagat cactgctagt
    22861 caggcatggc aaaacgcaaa aactttagcg aaaccagtta ttccttcaca gttttctttc
    22921 tcgtcattag tagatacgct tgctaatgtg aagatagaaa aggcgaattt tttagaattc
    22981 tttggtggaa aagagggatc tattctagat cgatttcatg gggaatttct aaaagataat
    23041 aacacattac gtcatgaaaa aaggctaggc acggatcata aaatcaaagc gatttatact
    23101 aaaaacttaa ctggtcttga cttagagatt gatgctcaaa gtgttttagt tggagtttat
    23161 ccattcatta gcagctcttc agaaggagaa gacgagatca ctctaccaga agaagttatt
    23221 ttcacggatt acgtggatga ttatcctgct ggatatgttt cttttgttga ttttaaagac
    23281 aaagcgactg atgtagccac attaagggaa gctgctaaag actggttgaa aacaaacata
    23341 gataaacaaa aaccacaagt gagtggttcg attgaattag taccattgag gcatcaaaga
    23401 ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg gtgacggagt agatgtgtat
    23461 catccacagt taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt taatgttcta
    23521 accaattcat acgataaatt agttgtagga aacgtcaaaa caaacttctt agaaaacaca
    23581 gagaataatg tcagcaattt gattaatgat gccattgatc aattgaaaaa cggtggcgaa
    23641 atcagtgatt tactcaatga tattgtagat catcaaactg atatgattac tggtcaaaat
    23701 ggtggttatg ttttattaga tcctaaagaa gcgcctagtc gtattttgat tatggacaca
    23761 ccagataaga ataccgcaag gaatgtttta caaatcaaca atgctggtat tggtttttct
    23821 aaaactggca ttaatggaac atatgaaacg gcatggacgt tagatggcgg attcaatgcc
    23881 tcgtttatta cggctggtga aatagtagga attactatta gaggtactac attaattagt
    23941 gatggtgctg attatagaac aagtattgct aatggcaaaa tgacttggta ctcaaaaaaa
    24001 gttaacaaag atattatgga gctagaagca cgtgattatg taagtgctga tgccggtatt
    24061 gtatcataca ccatgaaaac tggtggtggt ttcatgatta gaaatccaca gggtaacttg
    24121 gtttttagta cgtgggataa tggtaataac agaccgtttc tatcttttgg tgcgcccaat
    24181 ttcaggtata gcaatgctag ttatgtaact tctggcgacg gtagttcttt aagcattaat
    24241 ggtagtgcgg gtaactcatg ggaatttaag gtagctggta ggactatgaa atttactagt
    24301 gatggtatgc taacgttacc aggttgtttt tttggttcat gggaagatgg gaaacttgct
    24361 aggtttgaac aatcaacggt acaagtatat aaagatttta ctgttagagg tactaaaaac
    24421 tcaactgtac caacagaaca ttatggacaa cgactattga acgcttatga aactccagaa
    24481 tattatttcg ctgattatgg ggaagccgtt acaggtgacg atggtaaagt tcgtgttgat
    24541 attgacccca tgtttgctga aacagtaaat ctaagtcggt atatgacaca tgtgacacct
    24601 acagaactag ttttgtgtgc tgttactcat gaagatattg accatttcat cattgaaact
    24661 agtaagccaa acgtattagt tagatggaat ttagtggcac accgtctagg gtatgaagat
    24721 attagattaa aagaggatac agcatatgat agcacagtgc ttgaccaaaa acgtttttaa
    24781 aacgaagaca aggaggtata taaatggcta gcagtttata taatttggct ttagatttca
    24841 gcaaagaatt aaactacacc aaagctatta tggctcgtca aggtgataaa gggattacgg
    24901 tgacggttaa accgtttcta aatggcttgc agatggatac gagtggcgga acatttactt
    24961 taaaaggaac aacaccatct aaccgttacg tagataatgt tgcaactagt gtaactagtg
    25021 aagaagtcac gttttctctt gatggcacat ttatgagtga agcaggatat tataaacact
    25081 gctacgtaga atatagaaaa gacaatcaaa ttttaacaac gcaagatatc atttttttct
    25141 cactaggagt gtctgacatt tcgcaaggcc aagccgatga atatgtttcg caattagaag
    25201 agttgattcg aaagtacaac gaaacttttg atgcttttat ggctgaaatc aaaggtagag
    25261 tggatagctt aaatcaacag attactgatt taactggtca agctaaaacg ctacaagaca
    25321 agttagatgc tctgaaagaa gaaatttcta agttaggtaa cttacaagtg atgtacagta
    25381 acagcatcga cttcggggga tatgattata gcggtaagcc gaatttaatg tccaaactaa
    25441 aatcgagcga ttttaacgtt ggttaccacg ggtcactaac tttggataac gaaaagttac
    25501 attttacttc tgatggtaca ggaagcatta ttatgtttac gcgtattaat acacctcagc
    25561 ttactagtgg gaaaacctat actctgagtg cgaaagttcg atttgatgaa ggaactacag
    25621 gagctattga taaattacgt ttggtgtatc gtacatcacc aggagaaaag atattattgg
    25681 aagcaaatag tacaaatatt acaacagatg atgtagggaa agaaataaca atcaaaggta
    25741 cagctaacgt taattatcaa atcacaaatt tagatcgatt ttatatgagt attagctttg
    25801 ttgacaggga taaaataaat ggcggattta agttgtacga catcaaaatc gaagaaggct
    25861 caacagccac accgtaccag cctaatctac ttgatgcacc gtattatttg agtaaggtgg
    25921 ctttgggtga gaatctaatt aaaccagaat cacaacaacc agttactaat agtaactatc
    25981 ttattaacac ctataacatt aaaccaatgg taaaaggtaa gaagtatacc atcacacttg
    26041 aaggaactaa gccagcaaca caggttttta gaccattttt cacacgagca acaggagatg
    26101 catgggaggt tggtgactta caaccagtag aaggcttaac taatgtgtgg tctaaaacat
    26161 ttacagcagc agatgactca caccctacta ccccacaagt gcagatttat caagtaccaa
    26221 gcacaagtgt aggacaatgt acaattaagt ggttaaaact agaggaaggc aatactcgaa
    26281 ccccgaatat tagtgagtat aaatatcgtg gtactggtat gcgtgattca aacaatccaa
    26341 aagattatgt ttgggatcta gcaccagaat atgtcgaaga taatttggcc acagatatta
    26401 aaatttctga aattactggt aaagcaaaca attataccga tgggaaagta tcggagatta
    26461 attcgcagtt gactgcttca attaatgaag tagacaccac agctaaggat gctcaaacaa
    26521 aagcgaatgc taatgcgact gctatagatg aattagacaa taagatcgat gaacgcatta
    26581 atgatacagc tactaccaca ttaacagtta caaacgggaa taccggatca gcaaagcttt
    26641 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa cgcagtggtg
    26701 gaaatgattc aacgatacta acaattccag aaggctatcg gccaccaatt agttttgagc
    26761 aactggttgg ctcgatagac cgttctactt tgaacagtgc tcagttatct attggtgcag
    26821 atggagccat taaatggcga agaaactcaa gttatggatc ggattatacc tttgcaatta
    26881 cttacacgat ttagaaaagc gtgaatcgat atgaaggcag catatagacc aattgaacct
    26941 tacggattcg agcaaatcat tgtgaatgat gaagaacatt taccggaaga atgcacagaa
    27001 gtcgaaccac cgattccaaa ttggaaaccg aaattcaatt actgggaggg aaataaatga
    27061 aaaacatttg gaaatatgga cgtactggcg gagagtacgc aggaaaagta ttggacgaca
    27121 tgcttgtatc cgttccttac acagatcagc caccgcttga aggaattcgt gctgatggcg
    27181 aaccgttaac aatcgctgat caaatgtttg atcctaaatt gaatcaatgg attattttag
    27241 cgaacgcact agatcacaac gatttaaaca atctcaaagc aatgtatgag tcgttagaaa
    27301 atgagaacgg cgatttaaaa cagatcaatg ccaaactcat gctaagcgat gtagcaatta
    27361 aacaggaaaa tactgcattg aaagaaaaag cggatagttt agcacaaatc aattcaaaaa
    27421 tgatgcttgc ttcgttacaa aatagcaaag acatttcaga aattaaagag caactaaatc
    27481 cagcttcaaa gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat
    27541 tggggctgtt tcactaacga acaagtaatg gttttcgttc cgttgtgcat tactgaagaa
    27601 aaagcagata aaatcattag caaagaagag agcgcatctt aattgatgtg ctttttattt
    27661 tgattcaagg agttgtcaca tgattaattt aggggaatgg ggagcgatag caggatcaat
    27721 aaccgctatc gtttctttga ttttattagt aataaaacca attactgcat ctttctcgaa
    27781 gattactgag actctttcaa aagtaagtca caatttagat ttgctgacta aagatttaga
    27841 atcgagcaaa tcagatcgat tgatgattca tgaagaacta aagaaacacg atgaaagatt
    27901 agatacacat gcagaaaaat tggtagaaca cacgcaacaa attaaaactt tatttaggga
    27961 gagaagaaaa tgaataataa aacgttcgaa gtactaaaat ggttcgcact ggtaattatt
    28021 cccgcactag ctactttcgt ggggttagtt ggtaaagcgc tcaattggca gtacacagat
    28081 atctgtgttg tcatcattac tggttttggc gcgtttttag ggagtgtgtt gggtgtatca
    28141 aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag gaggaaacaa atgaaaaaga
    28201 aaattactat tactgcgatg agcctgttaa cggctctttt tttattaccc attaatacgt
    28261 ttgcttatac tattaatgac gagtataatt tagcgccgaa tcaaggagac tccagattag
    28321 caattcctaa caaaattatt ttgcacgaaa ctggaataga tgcaccagca agaaacgtag
    28381 ccgccaacat gaaaaataat tataacggaa gcaattctta tactacagat gttattggtg
    28441 acggtgggat tgtttaccgt gtgggtgagc aaggatatgt ttcgtgggga gctggtaacg
    28501 ctaatcctta tgcgcctgta cagattgaat tacagcgcac atatgataaa gcattgtttg
    28561 aaaaaaacta tcgagcttat attgaatata caagagatag tgcaaaaaaa tatggaattc
    28621 cattgactct tgatcaagga acttctttat ttacaaaagg aatcatttct catttgtggg
    28681 tgacaaatta tgtttggggg aaccacacag atccatatgg ttacttatcg caaatgggag
    28741 ttagcaaaga aaagcttgct tatgatttag ctcatggatt taccgatgaa aatccaacaa
    28801 cttctgaaaa caagcctgtc attgatccaa cacgagctgg tgcagctaat cctacgctga
    28861 cagatggaac aaatcacgcc cacattgatc agtttgggga aatcgaaaac gcaaacttgc
    28921 atgtggctgg atggcacatt gctaactata aatacgagta tattttcatt atggactaca
    28981 atactgggaa agaattagct cgagtaagag ctgatggaat ttatagacca gatgtaaacc
    29041 aagcttataa tacttctgga aatgttggtt atcatgtatc tttcaatatg cgtaattttc
    29101 ctagtaagaa agtctatgta atgatgcggg caacgaatga tccagaggga aacactaaag
    29161 gcggtgcgca agatttccat gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat
    29221 agctcctcgt tgaggagcag tacataacta tattgacaac tataaaaatt attcgataaa
    29281 atagtgatgt tatcgcatat cttcactatc acccataaat agtcacactc caagctatgc
    29341 gataacaggt ttgttgccac acattctact ggttgattgt ttatggcttt atgtggcaac
    29401 aaccagtacc cttagctcag ttggtcagag cagacggctc ata
//
LOCUS       DAFGFZ010000024        29269 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000024 DAFGFZ010000000
VERSION     DAFGFZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.024"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            5..637
                     /locus_tag="LR726_000913"
     CDS             5..637
                     /locus_tag="LR726_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216020.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(771..896)
                     /locus_tag="LR726_000914"
     CDS             complement(771..896)
                     /locus_tag="LR726_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6216021.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1030..1923)
                     /locus_tag="LR726_000915"
     CDS             complement(1030..1923)
                     /locus_tag="LR726_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL6216022.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2105..3031)
                     /locus_tag="LR726_000916"
     CDS             complement(2105..3031)
                     /locus_tag="LR726_000916"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL6216023.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3116..3877)
                     /gene="pflA"
                     /locus_tag="LR726_000917"
     CDS             complement(3116..3877)
                     /gene="pflA"
                     /locus_tag="LR726_000917"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL6216024.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4121..6352)
                     /gene="pflB"
                     /locus_tag="LR726_000918"
     CDS             complement(4121..6352)
                     /gene="pflB"
                     /locus_tag="LR726_000918"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL6216025.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6629..9079)
                     /gene="parC"
                     /locus_tag="LR726_000919"
     CDS             complement(6629..9079)
                     /gene="parC"
                     /locus_tag="LR726_000919"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL6216026.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9091..11136)
                     /gene="parE"
                     /locus_tag="LR726_000920"
     CDS             complement(9091..11136)
                     /gene="parE"
                     /locus_tag="LR726_000920"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL6216027.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11302..11736)
                     /locus_tag="LR726_000921"
     CDS             complement(11302..11736)
                     /locus_tag="LR726_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL6216028.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11863..12501
                     /gene="plsY"
                     /locus_tag="LR726_000922"
     CDS             11863..12501
                     /gene="plsY"
                     /locus_tag="LR726_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL6216029.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12633..13508)
                     /locus_tag="LR726_000923"
     CDS             complement(12633..13508)
                     /locus_tag="LR726_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL6216030.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13590..14381)
                     /gene="codY"
                     /locus_tag="LR726_000924"
     CDS             complement(13590..14381)
                     /gene="codY"
                     /locus_tag="LR726_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL6216031.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14399..15799)
                     /gene="hslU"
                     /locus_tag="LR726_000925"
     CDS             complement(14399..15799)
                     /gene="hslU"
                     /locus_tag="LR726_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL6216032.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15813..16361)
                     /gene="hslV"
                     /locus_tag="LR726_000926"
     CDS             complement(15813..16361)
                     /gene="hslV"
                     /locus_tag="LR726_000926"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL6216033.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16398..17303)
                     /gene="xerC"
                     /locus_tag="LR726_000927"
     CDS             complement(16398..17303)
                     /gene="xerC"
                     /locus_tag="LR726_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL6216034.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17501..18877)
                     /gene="trmFO"
                     /locus_tag="LR726_000928"
     CDS             complement(17501..18877)
                     /gene="trmFO"
                     /locus_tag="LR726_000928"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL6216035.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18846..20924)
                     /gene="topA"
                     /locus_tag="LR726_000929"
     CDS             complement(18846..20924)
                     /gene="topA"
                     /locus_tag="LR726_000929"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL6216036.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21046..21903)
                     /gene="dprA"
                     /locus_tag="LR726_000930"
     CDS             complement(21046..21903)
                     /gene="dprA"
                     /locus_tag="LR726_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL6216037.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21959..22726)
                     /locus_tag="LR726_000931"
     CDS             complement(21959..22726)
                     /locus_tag="LR726_000931"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL6216038.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22719..23579)
                     /gene="ylqF"
                     /locus_tag="LR726_000932"
     CDS             complement(22719..23579)
                     /gene="ylqF"
                     /locus_tag="LR726_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL6216039.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23839..24237
                     /locus_tag="LR726_000933"
     CDS             23839..24237
                     /locus_tag="LR726_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL6216040.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24516..25016
                     /locus_tag="LR726_000934"
     CDS             24516..25016
                     /locus_tag="LR726_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL6216041.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25099..25650)
                     /gene="lepB"
                     /locus_tag="LR726_000935"
     CDS             complement(25099..25650)
                     /gene="lepB"
                     /locus_tag="LR726_000935"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL6216042.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25802..26029)
                     /locus_tag="LR726_000936"
     CDS             complement(25802..26029)
                     /locus_tag="LR726_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL6216043.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26026..26544)
                     /gene="msrA"
                     /locus_tag="LR726_000937"
     CDS             complement(26026..26544)
                     /gene="msrA"
                     /locus_tag="LR726_000937"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL6216044.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26564..27277)
                     /locus_tag="LR726_000938"
     CDS             complement(26564..27277)
                     /locus_tag="LR726_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL6216045.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27280..28176)
                     /locus_tag="LR726_000939"
     CDS             complement(27280..28176)
                     /locus_tag="LR726_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL6216046.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28334..29176)
                     /locus_tag="LR726_000940"
     CDS             complement(28334..29176)
                     /locus_tag="LR726_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL6216047.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         7723 a         6145 c         5041 g        10360 t
ORIGIN      
        1 tattatggaa attcaacgta gaaccactaa ttttttacat ttaggctctt tttttgcaac
       61 tttgacgatc ttattttttc tatgttattc ataccttaag acctcacatg cgccttctag
      121 tgcattaggc tggttgcttt tggatcattc tgatatcaca gaagccgaag aaaatccgtt
      181 ttttttgatt ttgtcttctc tttgcggctt actgttttct gtttatttcg gtgcctattt
      241 ttttcatcga catcctggta cgttaaacga agcttcaagc gtattgattg catttaaaac
      301 gttttctttt ttagcaattc ttttttattc gtttaatcac aatacgcttt tcttctttat
      361 catgaatggt ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt
      421 tagccaattg aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag
      481 cttgttttcc ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt
      541 cgcaggaaat ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag
      601 aaaaaacaaa caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt
      661 ttttagtttt aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga
      721 ctcgccattc gtttcttagt gattctggat tatcaaatgg acttcctaat ctaatcagct
      781 agttcttccc ataaaatgcc atacttgact gaatcataat attgattatt taaaaatcta
      841 acttttctta tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct
      901 ttgtttccag accatgtagt gaatctaata ggtggtagat aggcaaaata ctcaaataat
      961 tcttttaacc ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt
     1021 actgctctct taggttttac tcactaaaac tgttccatat tctttgatat aagaaaatgc
     1081 atgcggtata aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccatttc
     1141 ctccattgtc aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac
     1201 aataggtaat tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga
     1261 ctgatggaaa atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat
     1321 aacttctatt tgatcaaaaa agtacgattc atttacgtct ccaataagtt cggctaattc
     1381 tttgaatgcc ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt
     1441 aagcgggaag tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt
     1501 tgtcctcagc aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg
     1561 ttcatcttcg aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac
     1621 agagagtctc ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta
     1681 atctttagga tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa
     1741 tcctgcatcg atcaataaat caagaaaatg ggaaaattta ttttgcgaaa attctttttt
     1801 caattctctt agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga
     1861 aaaaagcgga tcatcggtca gtttttgata aaagttttcc ttattttttg aataatagtt
     1921 catatatgtt ctcttttcta tccgttttat tttacttaaa aggcttttac gtaaaaagga
     1981 ggaagaacaa gagtcataat tgactgttat tctcctccat ttcttcggcc tttagtatgt
     2041 tctatgtttc tttttatatc aatcgcttat ccattcattt tgaatgcttc atactaccat
     2101 ttgattagct aaatacagcc gtcaattgag gtacgacttg ttttttacgt gaaacgacac
     2161 ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa agcttcttct actttttctt
     2221 ttcctttacc agcaacaagt aattctgaat cactgtctaa gatgtttgtg acaactaata
     2281 cgaacagatc gtagccattt gctttcattt catcttccat tgctgcttct agctctgctt
     2341 gtcgatccat cacttcatta aggtccactg tattgacttg agcgatacga acgttggaac
     2401 tgcccattgg gaaacttttt gcatccaaat caagaagaac cgcagctgat ttatcactta
     2461 gatttgttcc tgctttcaac atttcaagtc cataagtgtt tagatcgact tcagcgattg
     2521 ctgcaagttc ttttgcagct gcttcatctt caggcgtaca tgttggtgat ttgaacaaca
     2581 atgtatctga aatgatagct gagagcatga ttcctgcgat ttctttagga attgttactt
     2641 ggttttcttt atatagtttt aaaataatcg tacttgtaca tccaactggt tctgcacgat
     2701 agtacaatgg attagctgtt tcaaagtttg caatacgatg gtggtctact acagctaaaa
     2761 tatttacttc gctgatatct gaaatacttt gttggaattc attatgatcc acaagcatga
     2821 catctgcatt ttctgcttta gctgactcta ctactcgtgg tgcagatagc tgaaagtggt
     2881 ctaatgcgta ttgtgtttct tcattaggtg tacctagagc aactgcttct gtttctttac
     2941 ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc gattgcatct gtatctggat
     3001 tttggtgacc aaaaactaaa atttttgaca tgtcattcgc tcctttgtga tttctcctat
     3061 ttaataatag caaaaaaacg gaaagaagca atacttatct tctttccgat gcgacctatc
     3121 gcgttaaata ttttgtatac tcttctgtat gaagcaatcg tttagcattt tcgacacgtt
     3181 cttttgtagg tggttcgatc ccttctaatg gatatttcaa accaagtgtt tcccacttgt
     3241 attttcccat cgtatgataa ggaagaatct ctactctgtc tacatttgac aaggtttgaa
     3301 taaagttgtc tagtcggatc aaatattcat cataatcact tcgctcagga actagtacat
     3361 ggcgaatcca aaccggtttg ttgatttttg acaaatattg agccatttct aaaatatttt
     3421 cattgtccca atgcgttagt tttttatgct cttcattatc gatgtgtttg atgtcaaata
     3481 acagtaaatc tgtatacttc atcaattctt gaaaacgtga aaagaaaggt tcttcatacg
     3541 taaaaggttt tccgcatgta tccaatgtag tatgcatctt ccgctctttt gcttttttaa
     3601 atagttcgat caaaaaatcg atctgcaata gaggttctcc accactaaca gtaataccac
     3661 ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc taaaagttca tctgctgtgt
     3721 attctttccc tccgccaata ttccaagtat ctggattgtg acaaaattgg caacgcatgc
     3781 ggcatccttg cataaacacg acaaagcgca gtccaggtcc gtctactgaa ccgaaagttt
     3841 cgatagaatg aacatagccg attgtttttt cttccattta agttcacatc ttttctttga
     3901 tttcatttta agtacaagtt agctgtcgaa gcgaagcttt ctgaacaaaa tttttcgcaa
     3961 ttgccgcttc gttatgtgta aaatttacag ggacggaaca tacaagtcct gcccctgtaa
     4021 atcgtttctt tcaaataagc atactgatta ttcgattttg atgcgatggc tattcaacgt
     4081 cctttcgacg ctaagtcgaa aaactttttt cacgaacttt ttacattctg tcgtgtgaag
     4141 ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc acggaattta actgcataac
     4201 cagatacccg gatcgtcaag ttcggatatt tcattggatt ttccattgca tcacgcaata
     4261 aatcattact gaatacattg acattcaaat gataagctcc ttttgagaag tatccatcta
     4321 agacattacg caagttttcg attcttgtat cttgttcttt tccaagacct gaaggattga
     4381 ttgtttgggt atttgaaatt ccatccaatg cacagccgta attcatttta gctgttgagt
     4441 tcaggctagc taataaccca ctttgttccg cattgtaact tggatttgct cctggcgcaa
     4501 gtggttttcc agcttcacgg ccatcaggag tatttcctgt atttttaccg taaacaacat
     4561 ttgaagtaat cgtcaataat gaagttgttg gttttgagtt gcggtaagtt ttatgacgtt
     4621 tgatttgtcc catgaagtat tccaagatcc atgaaactaa ttcatctgca cggtcatcat
     4681 catttccgta tttagggaag tcgccatcca cttcaaaatc aactgctatt cctttttcat
     4741 tacggattgc ttttactttt gcatatttga tcgctgacaa gctgtctgcc gcatgagaga
     4801 tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga atccatcaaa gccagctgag
     4861 ctgcttcata agaatattta tcatgcatat aatgaatgat gttcaacgta ttgacatata
     4921 gttctgctaa ccaatccatc atattcatga agttatgttt cacttcttca aagtctagta
     4981 tttctgatgt gattggacga tattctggtc caacttgggc gccagtcact tcatcgactc
     5041 cgccattgat ggcatataat agtgttttag ccaagttagc acgagcgcca aagaattgca
     5101 tgtcttttcc gacaactgtt cctgatacac agcaagcaat cgcacaatca tcagaacccc
     5161 attgttcacg aagctgatta tcattttcga attgaatcga agaactttcg atagcgattc
     5221 tgctagcata tgttctgaat ccttctggaa ggtgttcaga atacaaaaca gtcaagtttg
     5281 gttctggtga aggtcccata tttgttaatg tatgaagcag acggaaatca ttctttgtaa
     5341 ctaatgaacg accatctaat cccatacctg caatggataa agtagcccag atagggaatc
     5401 ctgagaataa ttggttatat tcaggtgtac gcgcgaattt gaccatacgt aatttcatta
     5461 ctaagtggtc gatcaattct tgtgcttctt tttctgtgat tactccatta tccaaatcac
     5521 gttggatata gatatcaagg aatgctgaga tacgtccgat tgacatcgca gcaccatttt
     5581 gtgatttgat tgctgctaaa tatccgaagt atagccattg gacagcttct tttgcatttg
     5641 cagctggttt agaaatatca tagccatatt tcgcagccat ttctttcatt tgcgctaatg
     5701 cacgatattg ttcagtgatt tcttcacgta aacgaatcac ttcttcactc attgtatgat
     5761 aaccagtcat atcatgatct tttttctttt gttccatcaa atagtcgatt ccatacaatg
     5821 ccacacgacg atagtcaccg atgatacgtc cgcgaccgta agcgtctgga agccctgtaa
     5881 tgattttgtt tttacgagca gcacgcattt cagcagtata agcgtcaaat acaccttgat
     5941 tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc atcgtttggt gtatagccat
     6001 tgctttttag cgcattgtta gccatattga ttccgccgaa cggcatgaat gcttgtttca
     6061 aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc tttatccaaa tagcctgcat
     6121 cgtgtgaagt aatagttgat acgatttctg tgtccatatc atagacaccg ttacgctcga
     6181 attggatatc atttaacttt tgcaattcat cccataattt ttctgttgct tctgttgggc
     6241 cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt attttgaata aagtctctag
     6301 tgtctacgcc ttctttccat ttggtacctt taaatccatt ccattgctcc attgtatgga
     6361 cctcctaaat ttctgtttta tgtaacagtt tttacgtgat tagtataaca cagcacccta
     6421 tctttgcaag ggttttcttg ttttttattt aacaattttt atgttttatt tacatatatt
     6481 taaatcaacg ttgacatacc aacatttaag cattgtgaac actttcatct ttttttgcaa
     6541 acgtataagg atttattaaa aatgaatgaa aaagagacgc cacctgtatt aatacagatg
     6601 gcgtctcttc attaaaatac tacaacagtt acgactcgat gaaaatctca ggagcatcga
     6661 taacttcgat gacttctcca ccttttttct catccatcgc aaatgatcca ttcgatgttc
     6721 gatcacctat agggaaatca gttgggtcaa tcgttaactg ctgccctttt tgattcatca
     6781 aggttatttc ttttgatctt ccttccgaca tatagataat acggtgcgga ttttttttca
     6841 gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt ttggctgatt tcttgagcaa
     6901 gcatgcgttt aacagctcct cgctgagtaa cgattacgat cggcgtatca ccttctgcta
     6961 acacaagcag cccgttgata atcctatctc catctttcaa attcatagac ttcactccag
     7021 ctgcctttgc tccaacaacg ggaacatctg ttaaaggata gcgcaaaccc attccttgat
     7081 gcgttactaa gaaaacatct aagcctggct gatcttcttt cagatacaca gcaatcagtt
     7141 catcttcttc cgcctttagc ttcatacagc tgagcggacg gctcttatag gtgcgccatg
     7201 gttcgaactc tgtcattcgt gtttgcttga tcaatccatt cttgctgatg aatacaaatg
     7261 ttttctctgc ttgaattttt tgatatggga aaacagctaa aatcgattcg tctacagaca
     7321 gattggagat tgtttgagaa atgtgctcac cagcatcttt ccatttcaaa tcgggtagtt
     7381 catggacagg gcggtagatc acatttgctt tatttgtgat cagaagaaca tggtccaatg
     7441 tactcaattc acctgagtat agcaagtagt cgccttctcg catcccgatt tcttctggtt
     7501 tggaagcact ataagaacga aggcttgttc gcttcacata gccttctcgc gtcacactga
     7561 caattacgtc ttcttgtgcc actaagactt gcgtatcaat tttgatttct tcgatttcgt
     7621 cttcgattgt cgttagacga gcggaagaat aatttttttt cacttctcgc aattcttttt
     7681 tcataacaga gaaaagctct ttgtcatttg aaaggatttt attcaactct gtgatttgcg
     7741 caatcaagct ttcggattcc ttttgaagct ctgtgatatc cgtgttcgtc aaacggtata
     7801 attgcaatgt gacaattgct tctgcttgtt cttctgtaaa ttgaaagacg tcgactaaat
     7861 tctttttggc gtcttttttg tctttacttt cacgaatcgt tgcgattact tcatccagaa
     7921 tcgataaagc tttcatcaat ccttctacga tatgctgtct tttttgagct ttggctaaat
     7981 cgaattgact acgttttgtt atgacttgct ttcgatgctc gatataactt cttaaaatgt
     8041 ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc gaccatattg aaattgtaat
     8101 tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat tccttgagca ttggcgtctt
     8161 ttttcaattc gacgacaatt tgcagacctg ttctatcact ttcatctcgt acttcagcta
     8221 ttccatcgat tttcttattc aaacggatct catccatttt tttcaccaag gtcgctttat
     8281 ttacttcata tgggatttct gtgatcacga tttgctgttt attacccttc attgcctcta
     8341 tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt ttcataagct tttttgattt
     8401 catcttttcc ttgcaagatc cctcctgttg gaaagtcagg tccaggaata tattccatca
     8461 atttttcaag gctcgcttgt ggatgatcga tcatatagat tgtgccatcg atcacttcag
     8521 ctaagttatg agtcgggatt tctgttgcat atccagcaga tatacctgtc gatccattga
     8581 cgagaaggtt tgggtatcga gctggaagga ctgttggttc tttttctgta tcatcaaaat
     8641 tccacacgag atcgactgtt tctttctcga tatctgctag catctctcca cttaacttgg
     8701 ataatctggc ttcagtataa cgcatcgcag caggtggatc accgtccata ctcccgttat
     8761 tcccgtgcat ttcgatcagt acttcccgca acttccagtc ttggctcatg cggaccatcg
     8821 cttcataaat actgcggtca ccatgagggt gataattccc catgatattt ccgacagatt
     8881 ttgctgattt acggaatcct ttgtcatacg tattgccatc tttgttcatg gcaaataata
     8941 tccggcgttg cactggcttc aatccatcac ggatatcagg cagtgcacgt tcttggataa
     9001 tatatttgga ataacgtccg aagcggtcac ccatcacttc ttctaaagtt aattcttgta
     9061 cttcttgtcg attttccatc aggtgacctc ctattctact tcaaataact gattgttttc
     9121 atttttatct gctcgttctt catctaataa atcattagaa actgaaggag agatttcagt
     9181 atcctctttt ttatctaaga tactgccatc ttcttcgagc gtaaattgca cgtgattttc
     9241 gatccatttt cttcgaggtt ctactttgtc acccatcagt gtggttactc gacgttcagc
     9301 ttgcgcagca tcatcgatac ggacgcggat caacgtacgg gaagtcggat ccatcgttgt
     9361 ttcccacaat tgttccgcat tcatctctcc aagtccttta tatcgctgca gcatatatcc
     9421 tttgccgact tttttaatca ttgctgccaa ttcgtcatct gtccacgcat attcaatgac
     9481 ttgcttcttt ccttgtcctt tggatacttt atataaaggc ggtaacgcga tatatacttt
     9541 cccagcttca atcagcggtt tcatataacg ataaaagaaa gtaagcagta atacttgaat
     9601 atgggcgccg tctgtatccg catcggtcat gatgattact ttgtcgtaat tacagtcttc
     9661 gatagaaaac tcaggaccga ctcctgcacc aatcgtatag atcatcgtat tgatttcttc
     9721 atttttcaag atatcttgca ttttagcttt ttcggtattg atgacttttc ctcgtaaagg
     9781 tagaattgct tggaactttc tgtctcttcc ttgtttagca gatccgccag cagaatcccc
     9841 ttcgactaaa tacaattcat ttttcttagg gtttcgggat tgtgcgggtg tcaattttcc
     9901 tgaaagaaga gattcgcctt ttttacgctt cttcccattt cggctttctt ctctggcttt
     9961 acgtgctgct tctctcgctt ctctggcttt gattgcttta cggaccaaca tctggctcat
    10021 ttcgctattt tcctgcaaat aaaagcccat ttgttcactg atgacgttat ctactacggt
    10081 acgagcaact ggcgtaccaa gtttttcttt tgtttgccct tcaaattgca gaagattttc
    10141 aggtacacgg atagaaagta cagcagctaa cccttctctg aaatcactac cttccagatt
    10201 cttgtcccgt tctttcagca aaccaacttt tctcgcatat tcattataag atttcgtcat
    10261 cgccgctttc ataccagctt catgggttcc cccatctttt gtccgaacgt tattgacaaa
    10321 agataaaaca ttttctgaat aaccgtcatt gtattgataa gcgacctcta cttcgatacc
    10381 ttctttttct cctgaaaaat aaacaactgg tgtcaatgta tctttttctt cattcagata
    10441 atcaacaaat tctttgattc cttcttcgta atgaaaaatt tccttgactg gttcttctcc
    10501 acgaagatca gaaagagaga ttttaacgcc ttttagaaga aaggcagatt ctcttaaccg
    10561 ttcggctaaa atttcatatg aaaatttcgt tgtactgaat attgtatcat ctggtaaaaa
    10621 atggacagtc gtcccgtttg ctttttttgt ttttcctatc tttttcaaag taccgtcagg
    10681 tttaccgccg tttttaaatt tctgctgata ctctacgcca tctctaacga tcgttacaat
    10741 tagccatttc gataaggcat ttaccacact ggctcctaca ccgtgcagtc caccagaagt
    10801 tttgtaccct ccttgaccaa actttccgcc agcatgcagc acagtaaaaa tcacttcaac
    10861 tgttggtatt ccggaagcat gcattcctac aggcatccca cgtccgctat ctgcaacagt
    10921 tatactattg tcttcatgga tggtcacatc aatctcactg ccataacctg ataatgcttc
    10981 gtcaactgca ttatcgacga tttcatagac taaatgatgc aagcctcggc tgtcagtgga
    11041 tccaatgtac atccctggtc tttttctaac tgcctctaat ccttccaata cttggatgga
    11101 cgagtcattg tattcgttgt ttactttttt tgccaaggca aacgctcctt caataatact
    11161 ttgcggcaaa accgcaccac gattatcata ctagaaaagg tcgtgaaaaa aaagagggta
    11221 gacgagaaat caattttaat tatctcctaa ccgctttttc gacattcccc tctatcgatc
    11281 atctcttctt tgtggtcatt tttatttttg gttttctttc aacttaccaa gttcaatctt
    11341 aatacaacga tccattacga tatctgtacg tcctgctttt cttagcatct cagctgcttc
    11401 tttgttttca atccctaatt gtgcccagaa cactttagca tcagttttca aaaaatcacc
    11461 tgctatttca ggtaaaaatt cactccgtct aaatacatct acgatatcga ttggatcatc
    11521 gatttctgtc aaattcgcat agactttctc gcctagaatt tcttcaccag ccaataatgg
    11581 atttactggt atgattttat atccattgtc ttgcaatacc tgtgcaacca tgtaacttgt
    11641 tcgttctggt ttattgctca agcctacgac ggcaatattt tttgcttctt ccaaatagtt
    11701 gaaaatcgtt tcttgtgttg gattttttac agacatttct gccacccctt gcttttcttt
    11761 ttattccatc tattatactt gtatctttca ttttttcaat tgttcctgtt acctattcat
    11821 gctaaaataa aggatagttt tcaacttgaa agagggtaaa aaatgaaatt gatcttatta
    11881 cttattttag catatctttt aggttctatt ccttccggtg tctggattgg caaattgttt
    11941 ttcaaaaaag atatccgaca acacgggagc ggtaatactg gaacgaccaa tacatttcgt
    12001 gtattaggca aaacagccgg gattgttgtc ctgcttatgg atattttaaa aggtacattg
    12061 gctacttgtt tgccgatgat cttccacctt tctgggatta accctctttg gtttggggta
    12121 tgcgccattc ttggccatac atttccgatt tttgctgggt tcaaaggagg aaaagctgtt
    12181 gcgacaagtg caggaatgct tttaggattt aatcctgtat tttttgttta ttcttgtatc
    12241 atctttatcg ttagtttatt ttgtacaagt atggtgagtt tgagcagtat gatcagtgca
    12301 gtattgatca ctttatctac gatcgtcctt ccttatgtag ctcctgtgat tttggctaag
    12361 cccaattggt tactgacgat cattgccttt ttagtttctt cctttatctt ttatcggcat
    12421 aaagacaata tcaagcgtat cagaaaccat actgaaagtc gaatcccgtt tggtttgaat
    12481 agcagcaaga aaaaatcctg atactttttc attccaaaga gataaaacgg gagtgtgaca
    12541 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
    12601 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
    12661 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
    12721 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
    12781 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
    12841 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
    12901 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
    12961 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
    13021 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
    13081 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
    13141 gtgatgccgt ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt
    13201 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
    13261 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
    13321 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
    13381 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
    13441 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
    13501 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
    13561 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
    13621 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
    13681 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
    13741 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
    13801 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
    13861 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
    13921 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
    13981 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
    14041 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
    14101 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
    14161 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
    14221 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
    14281 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
    14341 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
    14401 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
    14461 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
    14521 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
    14581 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
    14641 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
    14701 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
    14761 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
    14821 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
    14881 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
    14941 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
    15001 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
    15061 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
    15121 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
    15181 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
    15241 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
    15301 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
    15361 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
    15421 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
    15481 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
    15541 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
    15601 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
    15661 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
    15721 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
    15781 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
    15841 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
    15901 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
    15961 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
    16021 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
    16081 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
    16141 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
    16201 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
    16261 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
    16321 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
    16381 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
    16441 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
    16501 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
    16561 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
    16621 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
    16681 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
    16741 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
    16801 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
    16861 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
    16921 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
    16981 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
    17041 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
    17101 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
    17161 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
    17221 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
    17281 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
    17341 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
    17401 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
    17461 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
    17521 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
    17581 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
    17641 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
    17701 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
    17761 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
    17821 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
    17881 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
    17941 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
    18001 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
    18061 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
    18121 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
    18181 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
    18241 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
    18301 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
    18361 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
    18421 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
    18481 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
    18541 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
    18601 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
    18661 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
    18721 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
    18781 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
    18841 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
    18901 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
    18961 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
    19021 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
    19081 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
    19141 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
    19201 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
    19261 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
    19321 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
    19381 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
    19441 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
    19501 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
    19561 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
    19621 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
    19681 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
    19741 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
    19801 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
    19861 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
    19921 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
    19981 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
    20041 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
    20101 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
    20161 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
    20221 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
    20281 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
    20341 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
    20401 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
    20461 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
    20521 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
    20581 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
    20641 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
    20701 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
    20761 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
    20821 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
    20881 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
    20941 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
    21001 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
    21061 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
    21121 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
    21181 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
    21241 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
    21301 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
    21361 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
    21421 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
    21481 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
    21541 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
    21601 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
    21661 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
    21721 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
    21781 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
    21841 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
    21901 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
    21961 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
    22021 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
    22081 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
    22141 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
    22201 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
    22261 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
    22321 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
    22381 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
    22441 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
    22501 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    22561 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    22621 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    22681 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    22741 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    22801 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    22861 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    22921 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    22981 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    23041 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    23101 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    23161 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    23221 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    23281 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    23341 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    23401 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    23461 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    23521 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    23581 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
    23641 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
    23701 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
    23761 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
    23821 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
    23881 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
    23941 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
    24001 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
    24061 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
    24121 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
    24181 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
    24241 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
    24301 ctccgcctct atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt
    24361 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
    24421 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
    24481 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
    24541 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
    24601 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
    24661 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
    24721 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
    24781 tatatcataa aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc
    24841 cagtcagcct ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag
    24901 taatttttct aggtagattc atcaggaata tgatccaaat ccctattcaa ataatggttg
    24961 tttattcatt tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt
    25021 acgtactaag gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagac
    25081 tgcacgcttt attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa
    25141 tcttgcttta cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga
    25201 aactcttcga ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt
    25261 tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt
    25321 tttagttaga taaggttcct taactacttt cccattgatc ttcaattgat cattttggta
    25381 gctgacattt tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa
    25441 ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc
    25501 accttgcttc aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa
    25561 tccccgcaac atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa
    25621 gcgattaata tagcgttgtt tttttgtcaa tctctatcaa tccatccctc tttttttcat
    25681 tctttcttta ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata
    25741 attctttcaa ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct
    25801 cttatatgtc atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg
    25861 acggcaaata gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt
    25921 gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg
    25981 tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct
    26041 ccaattttct tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg
    26101 ctttttataa tagtcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat
    26161 ttttgtaacg attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc
    26221 agcaatgatc cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg
    26281 atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa
    26341 ttgttcatat gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt
    26401 cgtttggctt tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga
    26461 catcactgta tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc
    26521 tcctgcaaat attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt
    26581 ttttattttc ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt
    26641 tgtttgaagc atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta
    26701 ctaagtcgat tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc
    26761 taagcatgat agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc
    26821 tcttgatcat attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc
    26881 tagcttttag ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat
    26941 cgataggaaa acctagtact tcgaaagtac cattgagtag tgcttcattt tccaaataaa
    27001 atttatattc aatatcacta tctttttgat actcactaag gaaaaagctg atgatttgat
    27061 tgactttttc tttatttgaa ttgatcgtta agacaggatc tccttgtctt tccgtaagag
    27121 ctggtatttc tgggagttct ggttcccgat tagcaaaaat acggctgcct aaaaatacgg
    27181 tcgaaccaag tactacccca agaaggataa ggaaggcgaa tttccagtaa tttgttttct
    27241 ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt tattgatcat cctccttctc
    27301 taaccattgg ttttgtgtct cgatcagctg atctctcact gcatttgcca tcaattgata
    27361 acctaaatta tttggatgga aatgatcttc ttcatagagc acattatttt tgatgccatt
    27421 tagattttct gtcgtgctag ttgtttgatc tgttccttct tctgtgacac ctacttcctg
    27481 attcaatcct tgataaagca aatcattgat tgggataaag tagcagttcg gcattttttt
    27541 tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt gtctgcatgt cagtgatctc
    27601 aggaaagttc aaataaaatg gattgtagat gcctaaaatg taaataggtg cggttttatt
    27661 gagggatcga aattcttcca atagttctgt gacattttcc tgatagtttt tcagtggctt
    27721 tttaaaggaa ttgaccgtta acccgaataa attattttgg ataactttca tcaaatcatt
    27781 accgccaaca gtcagtgtga taaaatcagc tgttttgata ttgtttcgaa tttcttcttt
    27841 tgtttttact cgctttaaga tctggtcact tcgttcacca tttacaccat agttttctgt
    27901 ttcaaccgcg gtcaaattat aacggtcttt tagatcattt gcgacaaggg gaacgaatcc
    27961 tccttgcttg gtttgatctc ctactccttc agtcagcgaa tctccgatag ctatgtagtg
    28021 aagtttttct tttttatttg gatcttctct cacagtagtt actggtttca ttagtgggcc
    28081 agctttcggt atagaagcag ctaaacctgc atagatgatg atcgcaatta ataatgggaa
    28141 caaaacgagg atcaaatgtc gtttcgttaa tttcattttt tcacctcttg tattcgattg
    28201 gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt
    28261 attttcctaa ttttataaag aaaaagagaa taagctgcaa gcctttcagg ctgagggctt
    28321 atcctctttt cgactaatct acataataca tgattgcaaa cgcgtcttta ccagtatgcg
    28381 tagcgataac cggattcgta tgcagtacag ggatatgcat gtccgggaat aattcttgca
    28441 aaccctcttt gaattcttgc gccaattctt ttgcatccgc atgtgaaata ccgatttgtc
    28501 gcacatttgg caattctttc agttcatttt tcaaattatt aaaccattta tcgaacgttt
    28561 tctttcctcg ccccttagtt acagggatta attcagaatg ggcgaaattc atcaccacgc
    28621 gtatgttcaa aacactagac agcaaaccag ttgttcggct gattcttcca ccttttacca
    28681 agttatctag tgtggatatt ccgatgtata attttgtatt gtcttgtact tttttgactt
    28741 cttcaaggat tttctctact gttttacctt cttttgctaa ttcagctgct cgaattacct
    28801 gaaaagataa tccttggtca gtggtatcac tgtcgataac cgttactttc gtttttgtta
    28861 gattactggc ttgtcttgct gcttctactg ttccactcaa gccttttgtc atatggatcg
    28921 aaatcacttc actgccgtct tctcctagct catcatacat cgagatgaat tctccaattg
    28981 gtggttggct tgttttgggc agattttttg ctttcgccat catttccata aatctttcgc
    29041 cgtctagttg gtcatcatcg ggatatactg ttccgtcgat catgatggat agcggcatga
    29101 cacgaatgcc taaagcatcg cgtacatttt tttccatcgt acatgaagaa tctgttacga
    29161 tttttacatt tgtcattatt ttctctctct ctttctttaa caagatgcct tattcatgtt
    29221 aaactacatg taagacaatt tctattttta gtataacaga caaataatg
//
LOCUS       DAFGFZ010000025        29028 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000025 DAFGFZ010000000
VERSION     DAFGFZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.025"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            214..498
                     /locus_tag="LR726_000941"
     CDS             214..498
                     /locus_tag="LR726_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL6216048.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="LR726_000942"
     CDS             complement(779..1198)
                     /locus_tag="LR726_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL6216049.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="LR726_000943"
     CDS             complement(1200..1784)
                     /locus_tag="LR726_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL6216050.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="LR726_000944"
     CDS             complement(1831..3306)
                     /locus_tag="LR726_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL6216051.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="LR726_000945"
     CDS             3487..3798
                     /locus_tag="LR726_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216052.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="LR726_000946"
     CDS             complement(3913..6249)
                     /locus_tag="LR726_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6216053.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLPSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="LR726_000947"
     CDS             6391..7059
                     /locus_tag="LR726_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6216054.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="LR726_000948"
     CDS             7062..8105
                     /locus_tag="LR726_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL6216055.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="LR726_000949"
     CDS             complement(8342..9223)
                     /locus_tag="LR726_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL6216056.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="LR726_000950"
     CDS             9382..10110
                     /locus_tag="LR726_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL6216057.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="LR726_000951"
     CDS             10266..10583
                     /locus_tag="LR726_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL6216058.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="LR726_000952"
     CDS             10576..11184
                     /locus_tag="LR726_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL6216059.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="LR726_000953"
     CDS             11239..12030
                     /locus_tag="LR726_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL6216060.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="LR726_000954"
     CDS             12168..12656
                     /locus_tag="LR726_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL6216061.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="LR726_000955"
     CDS             12707..13579
                     /locus_tag="LR726_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL6216062.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="LR726_000956"
     CDS             13755..14117
                     /locus_tag="LR726_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL6216063.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="LR726_000957"
     CDS             14126..14878
                     /locus_tag="LR726_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216064.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="LR726_000958"
     CDS             14892..16694
                     /locus_tag="LR726_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216065.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="LR726_000959"
     CDS             16971..18011
                     /locus_tag="LR726_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL6216066.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="LR726_000960"
     CDS             complement(18063..20207)
                     /locus_tag="LR726_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL6216067.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="LR726_000961"
     CDS             complement(20564..21937)
                     /locus_tag="LR726_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6216068.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LR726_000962"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LR726_000962"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL6216069.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LR726_000963"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LR726_000963"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL6216070.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="LR726_000964"
     CDS             complement(23385..24398)
                     /locus_tag="LR726_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL6216071.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="LR726_000965"
     CDS             complement(24479..25078)
                     /locus_tag="LR726_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL6216072.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="LR726_000966"
     CDS             25264..25965
                     /locus_tag="LR726_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216073.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="LR726_000967"
     CDS             25975..28686
                     /locus_tag="LR726_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216074.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
BASE COUNT         8959 a         5225 c         5923 g         8921 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaaggaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atttaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgt
//
LOCUS       DAFGFZ010000026        27849 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000026 DAFGFZ010000000
VERSION     DAFGFZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.026"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            114..722
                     /locus_tag="LR726_000968"
     CDS             114..722
                     /locus_tag="LR726_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL6216075.1"
                     /translation="MFLAACQSNTNQGTSGNTSSSSEQRSSQVSDSSTKASTSSSSQT
                     KSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYFYKIEGVDDQKEYE
                     ARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAAKIAVDKVGSGTAT
                     DWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            806..1750
                     /locus_tag="LR726_000969"
     CDS             806..1750
                     /locus_tag="LR726_000969"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL6216076.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1885..3336
                     /locus_tag="LR726_000970"
     CDS             1885..3336
                     /locus_tag="LR726_000970"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL6216077.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3357..4193
                     /locus_tag="LR726_000971"
     CDS             3357..4193
                     /locus_tag="LR726_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL6216078.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4193..4852
                     /locus_tag="LR726_000972"
     CDS             4193..4852
                     /locus_tag="LR726_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL6216079.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4978..5667
                     /locus_tag="LR726_000973"
     CDS             4978..5667
                     /locus_tag="LR726_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216080.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5673..6629
                     /locus_tag="LR726_000974"
     CDS             5673..6629
                     /locus_tag="LR726_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL6216081.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6673..7185
                     /locus_tag="LR726_000975"
     CDS             6673..7185
                     /locus_tag="LR726_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6216082.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7441..7989)
                     /locus_tag="LR726_000976"
     CDS             complement(7441..7989)
                     /locus_tag="LR726_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6216083.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8078..9259)
                     /locus_tag="LR726_000977"
     CDS             complement(8078..9259)
                     /locus_tag="LR726_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL6216084.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9495..11003)
                     /locus_tag="LR726_000978"
     CDS             complement(9495..11003)
                     /locus_tag="LR726_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL6216085.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11162..11353
                     /locus_tag="LR726_000979"
     CDS             11162..11353
                     /locus_tag="LR726_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216086.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11556..12473
                     /locus_tag="LR726_000980"
     CDS             11556..12473
                     /locus_tag="LR726_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL6216087.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12466..13539
                     /locus_tag="LR726_000981"
     CDS             12466..13539
                     /locus_tag="LR726_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216088.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13687..14205
                     /locus_tag="LR726_000982"
     CDS             13687..14205
                     /locus_tag="LR726_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216089.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14186..14578
                     /locus_tag="LR726_000983"
     CDS             14186..14578
                     /locus_tag="LR726_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL6216090.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14654..15589
                     /locus_tag="LR726_000984"
     CDS             14654..15589
                     /locus_tag="LR726_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL6216091.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15722..16501
                     /locus_tag="LR726_000985"
     CDS             15722..16501
                     /locus_tag="LR726_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL6216092.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16494..16811
                     /locus_tag="LR726_000986"
     CDS             16494..16811
                     /locus_tag="LR726_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL6216093.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17055..18665
                     /locus_tag="LR726_000987"
     CDS             17055..18665
                     /locus_tag="LR726_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL6216094.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18822..19670
                     /locus_tag="LR726_000988"
     CDS             18822..19670
                     /locus_tag="LR726_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL6216095.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19901..20380
                     /locus_tag="LR726_000989"
     CDS             19901..20380
                     /locus_tag="LR726_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216096.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20413..22641
                     /locus_tag="LR726_000990"
     CDS             20413..22641
                     /locus_tag="LR726_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6216097.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22770..24413
                     /locus_tag="LR726_000991"
     CDS             22770..24413
                     /locus_tag="LR726_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL6216098.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24486..25031)
                     /locus_tag="LR726_000992"
     CDS             complement(24486..25031)
                     /locus_tag="LR726_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL6216099.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25054..25539)
                     /locus_tag="LR726_000993"
     CDS             complement(25054..25539)
                     /locus_tag="LR726_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL6216100.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25529..26341)
                     /locus_tag="LR726_000994"
     CDS             complement(25529..26341)
                     /locus_tag="LR726_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL6216101.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26480..27592)
                     /locus_tag="LR726_000995"
                     /pseudo
     CDS             complement(26480..27592)
                     /locus_tag="LR726_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(27613..>27849)
                     /locus_tag="LR726_000996"
     CDS             complement(27613..>27849)
                     /locus_tag="LR726_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL6216102.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         9112 a         5074 c         5564 g         8099 t
ORIGIN      
        1 ttaaacatga cagaaaattt tttcttcgtt atacttgtaa tagaatacta ttttataaca
       61 aaggggatcg aatcaatgaa aaaactgtta tttatactag tttatttgca gctttgtttt
      121 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
      181 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
      241 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
      301 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
      361 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
      421 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
      481 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
      541 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
      601 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
      661 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
      721 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
      781 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
      841 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
      901 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
      961 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
     1021 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
     1081 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
     1141 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
     1201 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
     1261 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
     1321 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
     1381 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
     1441 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
     1501 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
     1561 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
     1621 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
     1681 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
     1741 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
     1801 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
     1861 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
     1921 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
     1981 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
     2041 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
     2101 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
     2161 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
     2221 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
     2281 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
     2341 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
     2401 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
     2461 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
     2521 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
     2581 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
     2641 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
     2701 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
     2761 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
     2821 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
     2881 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
     2941 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
     3001 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
     3061 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
     3121 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
     3181 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
     3241 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
     3301 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
     3361 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
     3421 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
     3481 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
     3541 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
     3601 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
     3661 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
     3721 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
     3781 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
     3841 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
     3901 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
     3961 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
     4021 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
     4081 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
     4141 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
     4201 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
     4261 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
     4321 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
     4381 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
     4441 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
     4501 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
     4561 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
     4621 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
     4681 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
     4741 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
     4801 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
     4861 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
     4921 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
     4981 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
     5041 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
     5101 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
     5161 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
     5221 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
     5281 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
     5341 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
     5401 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
     5461 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
     5521 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
     5581 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
     5641 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
     5701 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
     5761 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
     5821 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
     5881 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
     5941 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
     6001 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
     6061 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
     6121 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
     6181 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
     6241 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
     6301 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
     6361 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
     6421 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
     6481 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
     6541 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
     6601 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
     6661 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
     6721 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
     6781 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
     6841 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
     6901 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
     6961 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
     7021 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
     7081 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
     7141 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
     7201 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
     7261 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
     7321 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
     7381 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
     7441 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
     7501 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
     7561 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
     7621 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
     7681 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
     7741 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
     7801 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
     7861 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
     7921 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
     7981 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
     8041 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
     8101 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
     8161 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
     8221 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
     8281 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
     8341 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
     8401 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
     8461 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
     8521 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
     8581 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
     8641 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
     8701 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
     8761 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
     8821 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
     8881 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
     8941 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
     9001 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
     9061 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
     9121 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
     9181 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
     9241 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
     9301 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
     9361 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
     9421 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
     9481 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
     9541 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
     9601 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
     9661 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
     9721 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
     9781 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
     9841 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
     9901 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
     9961 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    10021 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    10081 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    10141 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    10201 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    10261 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    10321 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    10381 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    10441 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    10501 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    10561 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    10621 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    10681 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    10741 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    10801 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    10861 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    10921 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    10981 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    11041 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    11101 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    11161 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    11221 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    11281 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    11341 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    11401 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    11461 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    11521 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    11581 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    11641 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    11701 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    11761 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    11821 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    11881 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    11941 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    12001 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    12061 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    12121 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    12181 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    12241 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    12301 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    12361 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    12421 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    12481 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    12541 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    12601 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    12661 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    12721 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    12781 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    12841 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    12901 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    12961 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    13021 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    13081 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    13141 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    13201 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    13261 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    13321 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    13381 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    13441 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    13501 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    13561 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    13621 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    13681 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    13741 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    13801 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    13861 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    13921 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    13981 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    14041 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    14101 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    14161 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    14221 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    14281 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    14341 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    14401 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    14461 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    14521 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    14581 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    14641 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    14701 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    14761 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    14821 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    14881 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    14941 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
    15001 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    15061 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    15121 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    15181 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    15241 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    15301 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
    15361 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
    15421 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    15481 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    15541 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    15601 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    15661 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    15721 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    15781 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    15841 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    15901 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    15961 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    16021 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    16081 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    16141 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    16201 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    16261 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    16321 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    16381 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    16441 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    16501 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    16561 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    16621 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    16681 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    16741 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    16801 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    16861 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    16921 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    16981 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    17041 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    17101 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    17161 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    17221 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    17281 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    17341 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    17401 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    17461 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    17521 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    17581 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    17641 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    17701 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    17761 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    17821 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    17881 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    17941 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    18001 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    18061 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    18121 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    18181 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    18241 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    18301 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    18361 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    18421 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    18481 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    18541 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    18601 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    18661 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    18721 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    18781 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    18841 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    18901 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    18961 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    19021 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    19081 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    19141 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    19201 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    19261 atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    19321 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    19381 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    19441 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    19501 cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga
    19561 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    19621 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    19681 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    19741 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa
    19801 tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt
    19861 tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt
    19921 aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt
    19981 aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt
    20041 cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga
    20101 attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt
    20161 ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat
    20221 tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga
    20281 agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt
    20341 ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag
    20401 gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa
    20461 aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc
    20521 gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg
    20581 gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa
    20641 cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc
    20701 ttattacctg tagtagattc taataaggaa caagaccaag aattactaga agctatttac
    20761 caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct
    20821 ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat
    20881 gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta
    20941 ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca
    21001 gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat
    21061 tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct
    21121 ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca
    21181 gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag
    21241 attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg
    21301 cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt
    21361 tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa
    21421 gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg
    21481 ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa
    21541 cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct
    21601 gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt
    21661 cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa
    21721 caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa
    21781 atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa
    21841 gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta
    21901 cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga gttactggat
    21961 aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc
    22021 aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct
    22081 tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc
    22141 gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca
    22201 ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct
    22261 tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca
    22321 aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa
    22381 tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat
    22441 tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa
    22501 ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac
    22561 ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa
    22621 ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg
    22681 agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc
    22741 aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag
    22801 cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag
    22861 acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct
    22921 ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc
    22981 aagatataga agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac
    23041 atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc
    23101 aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg
    23161 tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt
    23221 gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag
    23281 acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt
    23341 tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat
    23401 attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag
    23461 aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag
    23521 caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata
    23581 tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg
    23641 tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga
    23701 acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc
    23761 gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag
    23821 gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga
    23881 tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta
    23941 agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac
    24001 ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca
    24061 ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc
    24121 tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt
    24181 cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag
    24241 atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa
    24301 aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag
    24361 ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc
    24421 tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac
    24481 tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc
    24541 acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata
    24601 tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact
    24661 tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt
    24721 gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact
    24781 aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac
    24841 aactctttta acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata
    24901 tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag
    24961 tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct
    25021 gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg
    25081 ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt
    25141 tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata
    25201 gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga
    25261 tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc
    25321 ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa
    25381 tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac
    25441 aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc
    25501 aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa
    25561 taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat
    25621 gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat
    25681 tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt
    25741 ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc
    25801 cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt
    25861 tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag
    25921 cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg
    25981 aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa
    26041 tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg
    26101 aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact
    26161 gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg
    26221 cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat
    26281 ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca
    26341 taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat
    26401 tggcatattt tcatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga
    26461 tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc
    26521 gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt
    26581 tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag
    26641 gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg
    26701 ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt
    26761 cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt
    26821 aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt
    26881 aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta
    26941 ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca
    27001 tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc
    27061 cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca
    27121 aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac
    27181 ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat
    27241 gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg
    27301 cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga
    27361 atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga
    27421 aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt
    27481 tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa
    27541 atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagga atacccgtgg
    27601 tgctagttaa ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc
    27661 tcgacttaat agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt
    27721 aacagaacgt gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtctc
    27781 aactgttttt ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt
    27841 tttacgtgg
//
LOCUS       DAFGFZ010000027        27631 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000027 DAFGFZ010000000
VERSION     DAFGFZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.027"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(91..672)
                     /locus_tag="LR726_000997"
     CDS             complement(91..672)
                     /locus_tag="LR726_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6216103.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(805..1488)
                     /gene="rpiA"
                     /locus_tag="LR726_000998"
     CDS             complement(805..1488)
                     /gene="rpiA"
                     /locus_tag="LR726_000998"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL6216104.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPERLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1807..2220
                     /gene="rpsL"
                     /locus_tag="LR726_000999"
     CDS             1807..2220
                     /gene="rpsL"
                     /locus_tag="LR726_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL6216105.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2304..2774
                     /gene="rpsG"
                     /locus_tag="LR726_001000"
     CDS             2304..2774
                     /gene="rpsG"
                     /locus_tag="LR726_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL6216106.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2868..4955
                     /gene="fusA"
                     /locus_tag="LR726_001001"
     CDS             2868..4955
                     /gene="fusA"
                     /locus_tag="LR726_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL6216107.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5127..6314
                     /gene="tuf"
                     /locus_tag="LR726_001002"
     CDS             5127..6314
                     /gene="tuf"
                     /locus_tag="LR726_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL6216108.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6721..7029
                     /gene="rpsJ"
                     /locus_tag="LR726_001003"
     CDS             6721..7029
                     /gene="rpsJ"
                     /locus_tag="LR726_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL6216109.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7060..7689
                     /gene="rplC"
                     /locus_tag="LR726_001004"
     CDS             7060..7689
                     /gene="rplC"
                     /locus_tag="LR726_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL6216110.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7717..8340
                     /gene="rplD"
                     /locus_tag="LR726_001005"
     CDS             7717..8340
                     /gene="rplD"
                     /locus_tag="LR726_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL6216111.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8340..8630
                     /gene="rplW"
                     /locus_tag="LR726_001006"
     CDS             8340..8630
                     /gene="rplW"
                     /locus_tag="LR726_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL6216112.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8669..9502
                     /gene="rplB"
                     /locus_tag="LR726_001007"
     CDS             8669..9502
                     /gene="rplB"
                     /locus_tag="LR726_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL6216113.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9545..9823
                     /gene="rpsS"
                     /locus_tag="LR726_001008"
     CDS             9545..9823
                     /gene="rpsS"
                     /locus_tag="LR726_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL6216114.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9846..10193
                     /gene="rplV"
                     /locus_tag="LR726_001009"
     CDS             9846..10193
                     /gene="rplV"
                     /locus_tag="LR726_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL6216115.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10207..10863
                     /gene="rpsC"
                     /locus_tag="LR726_001010"
     CDS             10207..10863
                     /gene="rpsC"
                     /locus_tag="LR726_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL6216116.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10866..11300
                     /gene="rplP"
                     /locus_tag="LR726_001011"
     CDS             10866..11300
                     /gene="rplP"
                     /locus_tag="LR726_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL6216117.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11290..11478
                     /gene="rpmC"
                     /locus_tag="LR726_001012"
     CDS             11290..11478
                     /gene="rpmC"
                     /locus_tag="LR726_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL6216118.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11503..11769
                     /gene="rpsQ"
                     /locus_tag="LR726_001013"
     CDS             11503..11769
                     /gene="rpsQ"
                     /locus_tag="LR726_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL6216119.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11827..12195
                     /gene="rplN"
                     /locus_tag="LR726_001014"
     CDS             11827..12195
                     /gene="rplN"
                     /locus_tag="LR726_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL6216120.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12232..12540
                     /gene="rplX"
                     /locus_tag="LR726_001015"
     CDS             12232..12540
                     /gene="rplX"
                     /locus_tag="LR726_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL6216121.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12568..13107
                     /gene="rplE"
                     /locus_tag="LR726_001016"
     CDS             12568..13107
                     /gene="rplE"
                     /locus_tag="LR726_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL6216122.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13126..13311
                     /locus_tag="LR726_001017"
     CDS             13126..13311
                     /locus_tag="LR726_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL6216123.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13347..13745
                     /gene="rpsH"
                     /locus_tag="LR726_001018"
     CDS             13347..13745
                     /gene="rpsH"
                     /locus_tag="LR726_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL6216124.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13777..14313
                     /gene="rplF"
                     /locus_tag="LR726_001019"
     CDS             13777..14313
                     /gene="rplF"
                     /locus_tag="LR726_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL6216125.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14474..14830
                     /gene="rplR"
                     /locus_tag="LR726_001020"
     CDS             14474..14830
                     /gene="rplR"
                     /locus_tag="LR726_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL6216126.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14851..15351
                     /gene="rpsE"
                     /locus_tag="LR726_001021"
     CDS             14851..15351
                     /gene="rpsE"
                     /locus_tag="LR726_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL6216127.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15366..15545
                     /gene="rpmD"
                     /locus_tag="LR726_001022"
     CDS             15366..15545
                     /gene="rpmD"
                     /locus_tag="LR726_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL6216128.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15589..16029
                     /gene="rplO"
                     /locus_tag="LR726_001023"
     CDS             15589..16029
                     /gene="rplO"
                     /locus_tag="LR726_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL6216129.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16029..17324
                     /gene="secY"
                     /locus_tag="LR726_001024"
     CDS             16029..17324
                     /gene="secY"
                     /locus_tag="LR726_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL6216130.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17386..18033
                     /locus_tag="LR726_001025"
     CDS             17386..18033
                     /locus_tag="LR726_001025"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL6216131.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18224..18442
                     /gene="infA"
                     /locus_tag="LR726_001026"
     CDS             18224..18442
                     /gene="infA"
                     /locus_tag="LR726_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL6216132.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18475..18591
                     /gene="rpmJ"
                     /locus_tag="LR726_001027"
     CDS             18475..18591
                     /gene="rpmJ"
                     /locus_tag="LR726_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL6216133.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18609..18974
                     /gene="rpsM"
                     /locus_tag="LR726_001028"
     CDS             18609..18974
                     /gene="rpsM"
                     /locus_tag="LR726_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL6216134.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19002..19391
                     /gene="rpsK"
                     /locus_tag="LR726_001029"
     CDS             19002..19391
                     /gene="rpsK"
                     /locus_tag="LR726_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL6216135.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19471..20409
                     /locus_tag="LR726_001030"
     CDS             19471..20409
                     /locus_tag="LR726_001030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL6216136.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20437..20817
                     /gene="rplQ"
                     /locus_tag="LR726_001031"
     CDS             20437..20817
                     /gene="rplQ"
                     /locus_tag="LR726_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL6216137.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21089..21928
                     /locus_tag="LR726_001032"
     CDS             21089..21928
                     /locus_tag="LR726_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL6216138.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21904..22773
                     /locus_tag="LR726_001033"
     CDS             21904..22773
                     /locus_tag="LR726_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL6216139.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22763..23560
                     /locus_tag="LR726_001034"
     CDS             22763..23560
                     /locus_tag="LR726_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL6216140.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23763..24509
                     /gene="truA"
                     /locus_tag="LR726_001035"
     CDS             23763..24509
                     /gene="truA"
                     /locus_tag="LR726_001035"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL6216141.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24964..>27631)
                     /locus_tag="LR726_001036"
     CDS             complement(24964..>27631)
                     /locus_tag="LR726_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL6216142.1"
                     /translation="KKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSIL
                     ASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPN
                     QMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMW
                     LDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLT
                     NWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLI
                     MKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFY
                     LVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAAL
                     FAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSA
                     EALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSAN
                     DSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNI
                     SKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLI
                     NTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQA
                     YLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTK
                     EISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDK
                     GWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILY
                     VWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9018 a         5141 c         5904 g         7568 t
ORIGIN      
        1 ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa aaaaacgaac
       61 agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga tttctttttc
      121 aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg aagaaataaa
      181 ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg tttttgcatc
      241 gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc caactaatgt
      301 acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt ggaaatagat
      361 ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca gcccgcgatc
      421 tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg
      481 gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca aatgaacagc
      541 agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac aaccaaagtc
      601 agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc gtgcataacc
      661 aataattttc atggtgattc ctttctagca gtgattttct aactggatat aacagtaaaa
      721 atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct gtcttttttt
      781 attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa tctcaacgcc
      841 tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat gttctactac
      901 cccaacaagt tcatctaaat aggtagctaa gcgttctggc tgttcgatga cttctagatg
      961 cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt cgtctgtacg
     1021 caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg agccataagg
     1081 gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag aagaatcaac
     1141 gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca aagcagcgcc
     1201 gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta ttgtcaaatc
     1261 gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg cttgttcttt
     1321 tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca tacggcgacc
     1381 aatctcttct accatataat aggcggttga accggtcccg agacctacga tcatcccgtc
     1441 ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct ttgcgtccac
     1501 ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta cggtaaaaag
     1561 ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct cttgttgaca
     1621 tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa
     1681 cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat ttatttttta
     1741 tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa
     1801 caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa
     1861 tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca aactaacgta
     1921 aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc taaaaaaccg
     1981 aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga agttactgct
     2041 tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt acgcggtggt
     2101 cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt agatactgct
     2161 ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc taaagcttaa
     2221 ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta ctatgaacaa
     2281 tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt
     2341 taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt gtaatggttg
     2401 acggaaaacg cgggattgca gctaacatta tctataatgc ctttgacttg atcaaagaat
     2461 ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc
     2521 ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt gaagttcgcc
     2581 ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg
     2641 aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg
     2701 cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt gcgtttgctc
     2761 actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc tcagatagtg
     2821 tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg accagagaat
     2881 tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct ggtaaaacga
     2941 ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt gaaactcatg
     3001 aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt acgatcactt
     3061 ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac acaccaggac
     3121 acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt gctgtaactg
     3181 ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaacaa
     3241 cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt gctgacttct
     3301 tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca atccaattac
     3361 caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa
     3421 tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa gaataccgcg
     3481 aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact gatgaagaac
     3541 ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc
     3601 gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca tttaagaaca
     3661 aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg ttagatatcc
     3721 cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct gcagacgaca
     3781 gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta ggtcgtttaa
     3841 ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa aacgcaacaa
     3901 aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct cgttcagaaa
     3961 tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac acaactactg
     4021 gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa ttcccagaac
     4081 cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa atgggtgtgg
     4141 ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac gtggaaacag
     4201 gcgaaacaat catcgctggt atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc
     4261 gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac cgtgaaactt
     4321 tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat
     4381 acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc gaatttgaaa
     4441 atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa actggtttga
     4501 aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc aaagctaaac
     4561 tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt gttgctgctt
     4621 ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa ccaatcatgg
     4681 ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac gtaactgctc
     4741 gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga
     4801 tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct actcaaggtc
     4861 gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca gtacaagaag
     4921 aaatcattaa gaaaaacggc ggaaaagctg aataatttct tgaaaccgga tagtcctgca
     4981 aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata attatcgatg
     5041 atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat aaaaaatata
     5101 ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac
     5161 acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta acagctgcaa
     5221 tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat caaatcgatg
     5281 gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt gagtatgaaa
     5341 cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac gttaaaaaca
     5401 tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct gctgacggcc
     5461 caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt ccttacatcg
     5521 ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa ttagttgaaa
     5581 tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt cctgtagttg
     5641 ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa attcttgaat
     5701 taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac aaaccattca
     5761 tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct acaggtcgtg
     5821 ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt gctgaagaaa
     5881 cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac tacgctgaag
     5941 ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc caacgtggac
     6001 aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca gaagtatacg
     6061 tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac cgtccacaat
     6121 tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga actgaaatgg
     6181 ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc gctatcgaaa
     6241 acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag
     6301 aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg gacgtaatcg
     6361 ctcggaacga aggtcttagg atctttgttc cgagcttttt tatttattgc aggatcagga
     6421 aaaaagacag gagtattata aatagaagaa acacgaaata aaagagtcag tattttctaa
     6481 atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt gtgagtttat
     6541 atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt aatcagaatg
     6601 atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc acgaacgagc
     6661 gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg agggaacaag
     6721 atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat tttagatcaa
     6781 tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc tggtccaatt
     6841 ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa atacaaagat
     6901 tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt gaacccaaca
     6961 ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa catcgaaatc
     7021 aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg aatcttaggg
     7081 aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc agtaactgtt
     7141 gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga tggctacgaa
     7201 gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc tgcgaaaggt
     7261 catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa aaatgttgag
     7321 ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc aggagacatt
     7381 attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa acgccacgga
     7441 caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt
     7501 ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat gggtggcaac
     7561 cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg
     7621 atcaaaggta atgttcctgg agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa
     7681 gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc attattcaaa
     7741 caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg aattgaacct
     7801 aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca
     7861 cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa
     7921 ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg aggtggcgta
     7981 gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta
     8041 gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc
     8101 ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc
     8161 gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt
     8221 aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga cgttgtatca
     8281 aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt gcttgcataa
     8341 tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg cttgccatgg
     8401 atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag
     8461 cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga
     8521 aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta
     8581 cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa gcactaatag
     8641 ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa
     8701 tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa cacctgaaaa
     8761 atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc gcatcactgt
     8821 acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca aacgtaacaa
     8881 agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat
     8941 cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta aaggactaga
     9001 agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa atgctcttcc
     9061 attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac ctggtaaagg
     9121 tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag aaggcaaata
     9181 cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac
     9241 aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag ctggacgctc
     9301 tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta atgatcaccc
     9361 acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt ctccttgggg
     9421 tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca aacttattgt
     9481 tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg agaggaggtt
     9541 caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt
     9601 tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac gccgttcaac
     9661 agttttccca agttttgttg gatataccat cgcagtttat gatggacgta aacatgtacc
     9721 agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac ctacaagaac
     9781 ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg ggaggataaa
     9841 tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt cgcacttcac
     9901 ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca gatgcaattt
     9961 caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag
    10021 cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta gtaagcgaag
    10081 cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt
    10141 ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa taaggaggga
    10201 taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat ccgcgactgg
    10261 gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga tttaagaatc
    10321 cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga aatcgaacgc
    10381 gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt tattggtaaa
    10441 ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg taaaagaatc
    10501 catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt aggtgaagga
    10561 atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa
    10621 cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg tctaaacgga
    10681 gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca tactttacgt
    10741 gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact aggagttaaa
    10801 gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa aggagggaaa
    10861 taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg
    10921 gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa gctgttgatt
    10981 cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact cgttacatga
    11041 aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact gccaaagcaa
    11101 ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca ccagtaaaac
    11161 gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac
    11221 gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg
    11281 gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat gcttgatcaa
    11341 gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac aggtcaatta
    11401 gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa aactgtgttg
    11461 cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga aggaagaaat
    11521 caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt
    11581 gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta ttctaagaaa
    11641 tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag aattatggaa
    11701 actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt
    11761 attatctaat aacacgagat tttcctatat agattgaatg caaaatctga aaggaggata
    11821 cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa
    11881 attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg
    11941 atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt
    12001 aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc atatattaaa
    12061 tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg aacacgtatc
    12121 tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt ttcactagca
    12181 ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt tatgtttgtt
    12241 aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga aggcgttgta
    12301 ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa
    12361 caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga agcgccaatt
    12421 catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt tggctacaaa
    12481 gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa
    12541 aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa
    12601 gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac acctaaagtt
    12661 gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa aaacttagat
    12721 aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac aaaagctaaa
    12781 aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg
    12841 cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact accacgtgta
    12901 cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta tactttaggt
    12961 attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa agtacgtggt
    13021 atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga gttgttgaca
    13081 caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc taaaaaatca
    13141 atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa
    13201 cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat ttgcttccgc
    13261 gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta aaaagtaaat
    13321 tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca gattttctaa
    13381 ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct gcatcaaaaa
    13441 ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat gttgaataca
    13501 tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac
    13561 gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg
    13621 acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact tctgaaggtg
    13681 ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat
    13741 ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc
    13801 gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac agttaaagga
    13861 ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat cgaaggaaac
    13921 gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca tggaacaact
    13981 cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa
    14041 ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt gaacgttggt
    14101 tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt accagctaac
    14161 acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt
    14221 cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt tggcgaatat
    14281 gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc atagaagcca
    14341 gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt tttacttgtg
    14401 ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca ataaaaataa
    14461 agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa agagacatcg
    14521 tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg tttttcgttc
    14581 taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc
    14641 ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg
    14701 taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg
    14761 tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact
    14821 agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa acatttggaa
    14881 ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt
    14941 ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg atttggtaca
    15001 ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac
    15061 ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg tgcttttagt
    15121 ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc tggtggacca
    15181 gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc tttaggctca
    15241 aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca
    15301 gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata aggaggacaa
    15361 caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt cctcaaaacc
    15421 aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg gttaagcctt
    15481 ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac gttgaagaaa
    15541 tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat gaaacttcat
    15601 gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca
    15661 tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc
    15721 ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt accaaaacga
    15781 ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt attgaaccgc
    15841 tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat cgtgaaaaac
    15901 gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt aactgtgaaa
    15961 gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg atcaatcgag
    16021 gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa
    16081 tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt actgtaccag
    16141 gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac atgttgaact
    16201 tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt tcaccataca
    16261 ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga tttgttgaat
    16321 ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga tatctgacat
    16381 tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa tacgctcaat
    16441 taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg attctaacag
    16501 ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt atcggaaatg
    16561 gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt atcagagaaa
    16621 tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct gctatttttg
    16681 tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt ttccaacaag
    16741 ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct acaagcagct
    16801 atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct agttcgttta
    16861 ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct gaaaactggt
    16921 acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg atttatacga
    16981 ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct gagaaattag
    17041 cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt aaggggactg
    17101 aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt
    17161 tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa tcaatcggat
    17221 taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg aaacagttag
    17281 aaggcttaat gctgaaacgt aaatatgttg gattcattga gtaagaaagc gtgttgaggt
    17341 ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa cctcatttta
    17401 atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga tgcttatggt
    17461 attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga aacagctctt
    17521 ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga agtaacaaat
    17581 ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt attagatggc
    17641 tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa
    17701 aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc
    17761 ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa ccctacaaaa
    17821 gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga agatgataaa
    17881 cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc aatcttggct
    17941 tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat cgatgctgta
    18001 tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt
    18061 atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc cgacttctag
    18121 agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt ttccgccatt
    18181 ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa aagaagatat
    18241 gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta aagttgaact
    18301 agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc attacatccg
    18361 tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta accgtggccg
    18421 tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat tatcatgaaa
    18481 gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc
    18541 gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata attggaggtg
    18601 ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt gtagtagttt
    18661 cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta gctgatgctg
    18721 gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat gctatccgtg
    18781 cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca
    18841 aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc
    18901 gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg
    18961 gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca aaaaaagtga
    19021 atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc cattctacat
    19081 tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg tggtcatcag
    19141 ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct caaatggcag
    19201 cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa gtaacagtta
    19261 aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca ggtttagaag
    19321 tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct ccaaaacgcc
    19381 gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt gaacaagaca
    19441 cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc aagaatcgca
    19501 aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga aagagggtat
    19561 gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc aggtgctgcc
    19621 atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa aggtgttaga
    19681 gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat gtatggccaa
    19741 gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc tggcgatatt
    19801 atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag tgtctctgaa
    19861 ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt tcaagcagat
    19921 gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat ctacacacct
    19981 gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga agattttgat
    20041 aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc aatgagtttg
    20101 tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga tgaagcaaaa
    20161 aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg
    20221 atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat
    20281 actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg taacttagga
    20341 cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg tttacgtaaa
    20401 gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa actaggacgc
    20461 acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa
    20521 cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc
    20581 actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat
    20641 gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa
    20701 ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac acgcattttg
    20761 aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact tgtctaaaat
    20821 cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt tggaagatat
    20881 ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga gtaagcactc
    20941 atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt ttgaaggcat
    21001 aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat agtgttagta
    21061 ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag aaaagataaa
    21121 ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct ttacaattga
    21181 taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga cacttgccaa
    21241 aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa atcaaatatt
    21301 ggatgaagaa aatatttgga cgatccgaca gatggttgga atggtttttc aaaatccaga
    21361 caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg aaaatcaagg
    21421 gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc
    21481 tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac gtgttgccat
    21541 tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa ccagtatgct
    21601 agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag aagaaagtca
    21661 attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca atcggatact
    21721 agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt tttctgctgg
    21781 tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga agagcgcact
    21841 gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga tggtggattg
    21901 gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac accatttgaa
    21961 caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac tgcattagta
    22021 gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct tgttaagcca
    22081 acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga taataagaat
    22141 ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc acagctattc
    22201 gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt cagcgaagaa
    22261 gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga aagctatttg
    22321 gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg
    22381 cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact tgatccccag
    22441 ggcagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca gatcactatt
    22501 gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt ctatgtgttg
    22561 gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa cattgaatgg
    22621 ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga attaatggct
    22681 aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga cgctatcgtc
    22741 cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt atatgcctgg
    22801 agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt tttattttat
    22861 cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat ttttcacatt
    22921 actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca ttcgcccatt
    22981 gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac
    23041 ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg gcctctttat
    23101 tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta ctacaccgcc
    23161 tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg tacggttccc
    23221 agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta cattgatgga
    23281 cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa
    23341 cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg tcagcagctt
    23401 caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag gtggggaagg
    23461 aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta tcattgcttt
    23521 tgtattatta actgtgctac tgatcttttt aagaggataa acgttcagtg ggccgctatc
    23581 tattcttttc ttgctgatct gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata
    23641 ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa agaaagaacg
    23701 aaaaaatttt atagaaaaat cattattcca gataaccttt taggaacaaa ggagagaaaa
    23761 acatggtacg ttacaaagca atcattgcgt acgacggaac aaattttaat ggattccaaa
    23821 aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa aaaatggcaa
    23881 atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta catgcgatcg
    23941 ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc
    24001 tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt tcagaagact
    24061 tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat atagggaaac
    24121 cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttacccactt gatttggaaa
    24181 aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct ttttgtgcat
    24241 caggaagctc gatagaggat aaaatacgta cgatctacga ggctaagatg gaggtaaatg
    24301 aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat aagatgatac
    24361 ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa gatagtatcc
    24421 cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacac cctgaaggac
    24481 tttatttgta tgaagtgaaa tatgaataag aggttatgat agaagtggtc agcttcaaga
    24541 aataagaagg tatttacgaa aattacttct caaaattttg caaaatttcg gcttattttc
    24601 gaagaagcta cttctggaac accgtttaag gaagaggtcg tgaaatcagc gttttgacct
    24661 gagtattaga gaaacaaacg ggaaaatagc tcctcatatt ttgccgtttg ttgagctaat
    24721 accgaaggtc agctgattga acaccgttta agaaagaggt cgtgagcctg tcatttaact
    24781 ctgagtcaca aagagcaatt tcaaaaaaca gcttctcatc tttgatgaat atcgtgactt
    24841 gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct gtaaccaaaa
    24901 aacgatggaa cgttgttttg attgcccttg gtgcggacag tctgaacagg ttccatcgtt
    24961 tctttatttt ctacgtcttc ttctgtgtgt gtttcgtggt tgttcttcta ttgctggatc
    25021 aatcgtgcca taaaaggggc gattgcggtc aaggtaccag acataaagaa taaacaatgc
    25081 aatacatagg acaaaaagga ttgcacctat cgtaaatcct tcaggcaagt aactgaaagt
    25141 gacagtatgt ttacctgctg gaacttttat catcaagaat gcgtctttga aattgctgac
    25201 cttgactggt ttcccatcaa ttttagcttt ccatcctttg tcataaggaa tagttgtcac
    25261 caacatggaa tcttcagcgg tatttacagt cccagaagct tttctgccag tggcttccat
    25321 ttcgacacct ttttctttga tctgattcat tgcggcttga taggctacaa catctagacc
    25381 aacgacttta ggctccatga aacttatttc tttcgtccca taaaaacttg cagtaaatgt
    25441 cactgttgtc tcttgaggat aatagccaag atcatagtat tgaccagaaa tgttgatttg
    25501 cgtttttcgc gatgtaccat tgacagtgat cgtcgcacta gaactttcta attgcgcaaa
    25561 attggttggg aacaaactta agtaagcttg tgtatttgca ggcacatgga cttcccaagt
    25621 gagctctttt ggctggttat ctgctgtttc tgagtaagtg acgccagtcg ttgtctttgt
    25681 gatcgtcgta ttcaactgat tcttcaaagt aggtgtatag aatgtaaaat actgttcatt
    25741 tagaccagac aatgtattga tcaagtttgt ctgactggtc aaattatctg tcactggctg
    25801 ctcaacatca ttaacggaca acggtgcagt aaatgctagt ggcaatgcat tgacattctg
    25861 atacagtgta tagtcgccgc tttgaccagc ttggcggaag ccatatttac tgaacgtgct
    25921 ttcgttttta gaaatgttat atttgattcc tgtaaaccca tccatcagca aagtattatt
    25981 gttataacgg atattcaaat ttgtccctct tgaacggaaa cctaatttat ccaggtaaga
    26041 ggaagaatta cgatttctta tagaagaaaa caaactgata ccactgtaac catagttgat
    26101 cgaatcgttt gcagatactg gatccagatt ttccaagcgg taaaacgagt cggaatcttt
    26161 ttttgtttca tttacaagag ctttcaagtc aggataagga tcagtatata aactgcggga
    26221 agcataattc caatcatcaa gaatgccatg gatcatactg ttcgtattga ccagtgcttc
    26281 agcagacatg atcaataata acaatatact cagatgattg aatgaaagtt ttttatgctg
    26341 ataaaatccc acagcaaaag cataaatccc taagaaaacc actgtcaata caaaggaagt
    26401 catcgtcaca taggtgtagc ttccttcagg tttgatgccc catgccaccg caaagagagc
    26461 agctacgacc aaaatgattc ctaacaacag cccacgatcg tcttgttcta tcttttccca
    26521 tccttttcct gcaagcagga gcactaagaa agagaacaga taggcataac ggaataaaaa
    26581 catattcggt gcatgcatac catgccagaa caaattcaat ggaacaagat aaaaacttgc
    26641 gataagtatg gcgaaaagac tgccgtacaa aaatttattt ttcaacggta ctttccgact
    26701 ggcaaaataa aacagacaga ggactaatgg tagcagacct gcgtaaacga acggaatcga
    26761 tccgtatttt gtcgtgtcat aaacaccaat catatttttc atgattagat ccagataaga
    26821 agtcgcttct gttttcaacg tagtgatctg actcagtgcc tcgccatttg accgaagatc
    26881 cagcactgct ggcaatacaa tgatcattga agcgccgcct gccagtaaag aagtgatacc
    26941 ataaggaaca atcgtttttt tatattctga ccagtttgtc aatagacggg cgataaaata
    27001 taagaaagaa aatacgccaa tcataaaacc catataaaaa ctggataaaa acaagagtaa
    27061 ataactgata aataaaagtt tcggccgttt aagatccata acacgatgaa tccccaaaat
    27121 cactaatgga agataaaaaa atgcatccag ccacatgatg atctccgaat gtgcagtgat
    27181 aaatgagatc aatgcgtaac tgacgctaag tgcaacatgc ccccagcgag gaatcttgaa
    27241 cgtacgtgca agaaaccaaa aagccaatcc ggcacagccg accttaagca aagtcagaac
    27301 atacagtgca tctggcatca tctggtttgg aaaaaagaac accagtggcg tgaagatccc
    27361 gcctagatag taagaaatta aagataaata attcaatcct agagaagcat tccatgtata
    27421 aaaaatactt tgctttccaa gaagaacatt tcggaagctg gcatgaaaat tagaaaactg
    27481 tgaaaaggca tcacttgcca aaatacttct gctgcttcca ggatagattc cgatacttag
    27541 ataaacaagt gctaaaatca aaaacggaat caggaaacta gctgctgcgt agcgtccgtt
    27601 ttctctaaag aatgacttta aatttttttt c
//
LOCUS       DAFGFZ010000028        26938 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000028 DAFGFZ010000000
VERSION     DAFGFZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.028"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(149..2302)
                     /locus_tag="LR726_001037"
     CDS             complement(149..2302)
                     /locus_tag="LR726_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL6216143.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2596..3444)
                     /locus_tag="LR726_001038"
     CDS             complement(2596..3444)
                     /locus_tag="LR726_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL6216144.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3668..5608)
                     /gene="thrS"
                     /locus_tag="LR726_001039"
     CDS             complement(3668..5608)
                     /gene="thrS"
                     /locus_tag="LR726_001039"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL6216145.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5945..6124)
                     /locus_tag="LR726_001040"
     CDS             complement(5945..6124)
                     /locus_tag="LR726_001040"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL6216146.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6171..7994)
                     /gene="cadA"
                     /locus_tag="LR726_001041"
     CDS             complement(6171..7994)
                     /gene="cadA"
                     /locus_tag="LR726_001041"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL6216147.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8272..10551
                     /locus_tag="LR726_001042"
     CDS             8272..10551
                     /locus_tag="LR726_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6216148.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10644..11291)
                     /locus_tag="LR726_001043"
     CDS             complement(10644..11291)
                     /locus_tag="LR726_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216149.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11470..12189)
                     /locus_tag="LR726_001044"
     CDS             complement(11470..12189)
                     /locus_tag="LR726_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6216150.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12320..13471)
                     /locus_tag="LR726_001045"
     CDS             complement(12320..13471)
                     /locus_tag="LR726_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL6216151.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13688..14428)
                     /locus_tag="LR726_001046"
     CDS             complement(13688..14428)
                     /locus_tag="LR726_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL6216152.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14432..14755)
                     /gene="rsfS"
                     /locus_tag="LR726_001047"
     CDS             complement(14432..14755)
                     /gene="rsfS"
                     /locus_tag="LR726_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL6216153.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14812..15405)
                     /gene="yqeK"
                     /locus_tag="LR726_001048"
     CDS             complement(14812..15405)
                     /gene="yqeK"
                     /locus_tag="LR726_001048"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL6216154.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15389..16039)
                     /locus_tag="LR726_001049"
     CDS             complement(15389..16039)
                     /locus_tag="LR726_001049"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL6216155.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16049..16360)
                     /gene="yhbY"
                     /locus_tag="LR726_001050"
     CDS             complement(16049..16360)
                     /gene="yhbY"
                     /locus_tag="LR726_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL6216156.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16376..17485)
                     /gene="yqeH"
                     /locus_tag="LR726_001051"
     CDS             complement(16376..17485)
                     /gene="yqeH"
                     /locus_tag="LR726_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL6216157.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17482..18009)
                     /locus_tag="LR726_001052"
     CDS             complement(17482..18009)
                     /locus_tag="LR726_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL6216158.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18189..18974)
                     /locus_tag="LR726_001053"
     CDS             complement(18189..18974)
                     /locus_tag="LR726_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL6216159.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18967..19836)
                     /locus_tag="LR726_001054"
     CDS             complement(18967..19836)
                     /locus_tag="LR726_001054"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL6216160.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19837..21216)
                     /locus_tag="LR726_001055"
     CDS             complement(19837..21216)
                     /locus_tag="LR726_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL6216161.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21224..21643)
                     /gene="fabZ"
                     /locus_tag="LR726_001056"
     CDS             complement(21224..21643)
                     /gene="fabZ"
                     /locus_tag="LR726_001056"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL6216162.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21668..22144)
                     /gene="accB"
                     /locus_tag="LR726_001057"
     CDS             complement(21668..22144)
                     /gene="accB"
                     /locus_tag="LR726_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL6216163.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22147..23382)
                     /gene="fabF"
                     /locus_tag="LR726_001058"
     CDS             complement(22147..23382)
                     /gene="fabF"
                     /locus_tag="LR726_001058"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL6216164.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23401..24138)
                     /gene="fabG"
                     /locus_tag="LR726_001059"
     CDS             complement(23401..24138)
                     /gene="fabG"
                     /locus_tag="LR726_001059"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL6216165.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24150..25067)
                     /gene="fabD"
                     /locus_tag="LR726_001060"
     CDS             complement(24150..25067)
                     /gene="fabD"
                     /locus_tag="LR726_001060"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL6216166.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25174..25398)
                     /locus_tag="LR726_001061"
     CDS             complement(25174..25398)
                     /locus_tag="LR726_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL6216167.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25451..26413)
                     /locus_tag="LR726_001062"
     CDS             complement(25451..26413)
                     /locus_tag="LR726_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL6216168.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26423..26863)
                     /locus_tag="LR726_001063"
     CDS             complement(26423..26863)
                     /locus_tag="LR726_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL6216169.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7349 a         5982 c         4687 g         8920 t
ORIGIN      
        1 tttttgtcac aacctttttt gccgagtaaa cggtgttcaa aagccagttt ctcggtatga
       61 agtcaaattt aagcaaaaat gtgagaagct attttcacaa aatttttctt catactcgaa
      121 actaagcggc tttttcacga cctctttttt attttgccat gaacatatca taatacgcgt
      181 ccattatttc tttagcaatc gtcaagttta tgtggtcttg ttcgtctaat aagttcggca
      241 agacaacact gatggcaatt tccggattgt ctgttggtcc gtatgccacg atattactat
      301 tgactgtcgc aatatctgga tgaccttccg caaccgtttc tgcagtccct gtttttgcgg
      361 aaaggtccat tttcgcactg gctaatggtt tggctgttgt gtaaggatcg gtaccatgga
      421 caacttggtg gaatccttca cggaggatcg ccatattttc tgcagaaata ttgaccgtat
      481 tttctactgt tgtgccaatt tctttcttca cttcacccaa gtttccattt tcgtcattgc
      541 catagatcgc tttgactagg tgcggctgta ctcgtttccc gccattagcg actgttgaag
      601 catattgagc taattggatt ggtgtatatg tgtcaaactg tccaaatgcc aaatcggtaa
      661 agttacctgg agcataccat tctttgccat tatccgcatt gaacttcttc atataatcaa
      721 cagaacgtga gatcccagga gattcattcg gcaaatcgat cccagtcgtt gtaccaagac
      781 caaactcttt gaaagagtct ctcaattgct gataagcttt agcttcttca tctaaactag
      841 gtagtcccat tccaggtgta tagtctagtc ctaacatctt taacgccact ttgatcatat
      901 acgtattaga agacaattcc gatgctttga ctgcatcaag agcaacttgt ccgcttcgat
      961 taaagacaga gcttttttct gaagcacctt gcagacgaat cggctcatca atcaaaactt
     1021 gatttccgct gatcgcgtta cttgcccagc ctgctgtcaa agtaccagcc ttgactacag
     1081 accctggatc aaaggcgcta gtaatcgtgc ctaaagcatt ctcggtgatt tctttggaac
     1141 cttgttcatg actgaaacca gtcattgcta gcacttctcc tgtctgagga ttcatagcca
     1201 ctgcataagc accaggcgaa tactgtgctt tgccgttgtt gatcaatgtt tgataattcc
     1261 ttttcaaaat ttcttctact tttgattgga attctgcatt catagataac attaaattag
     1321 aacctttttc cccagagaag atttcttttt gattcgaaac atttccttca ttatcaaggg
     1381 aaacttcata ttgggttttg gttccttgca ggacatcttc atattgtttt tccaaataac
     1441 tttgtcctac acggtcattc cgagaataac cttttgctag gtatttctct gcttcatctg
     1501 ctggaagtcc ttgcttttca gtcgtcacac tgccaagaat acttttcagt gaacttgctg
     1561 cattatattc tcgtgtccag tctgtgccag tcgaaactcc tggaagttca gaagcgcgtt
     1621 ctgccacaac agctaactct tgatcagtaa caccagagtt tttgatatag acagtgttta
     1681 attcatatgc actattcatc cgtttaaaga tggtagcaat ttttaattgc tcctcgttta
     1741 aattaatctc atcagctgtg actttattga ccatcgcact atattgttcg ctgttcccta
     1801 attgcttttc ttttgccgaa agtctttttg tggcttcttt caaatgtttg ggatcagcca
     1861 gccaaaaatc ttttttatct ctatctgtca gattttcatc taccggaaca tcgatcagcg
     1921 agttcagctt cgttgcgatt tcaagcaaat ctttagccgt aacttgtgtt cctctcgtat
     1981 atgtgatggc ctgattcgct ttattcgtaa ctaaagcttt tcctgtggag tcgtagatca
     2041 ttccgcgagg agtactttct tggaccgtaa tcgtagaagt agacttcact cgcgcctcca
     2101 tctcttcgcc gttcacgatc tgcaaataac ccaatctagc aatcaacgca acaaataacg
     2161 taaatataat gaaaaaaaga aaatttaagc gaaaaggaac atgggaccgg cgaaacgttt
     2221 tgttcttagg ttcttgtgca ccttttctta ttttttctaa ccaagaagta tttaatattt
     2281 ttttcataaa atctttcatc atttcctatc cttccgccta tttacattca tgcttcattg
     2341 tacccaaaaa cttcttaata aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa
     2401 tttcaagata aaatcaactt tttgtttcac agcaatcact taaagttaca tatccaacta
     2461 tttttttcat taaaaaataa acttattttt gaaaattatg ttttcttaag tcgtgaaaca
     2521 ctttgatgga tcatttattt gtggaaaact acgttttgtt attctaaaaa acttatataa
     2581 aaaatagtaa ggtttttatt tcgaatccat aaaatattct tggattgctt ctcgtgtttt
     2641 tttcactttt tcgctaagct tttcgttttt agccatatag aaataaaaaa tgatatcgat
     2701 cgtagcaaat tgggccaaaa gtgaattagt tgccgcactt cgattcgtga tttcttttgg
     2761 tctagatgtt tgtacgagta catcggctat cttgctaagc gggttatttc caaattgcgt
     2821 caacgcaatg atttcgatat tcatgctttt cgccagctcg gctaatgcga ctacatctgc
     2881 acttctacct gagtttgaaa caagccaaag cactttcttt ttctcattgc tgacaagttg
     2941 cgggagcaag acgtggatat ctttttcaaa aatgattggc ttaccgacac gagaccattt
     3001 ttgcagaata tcttctacta ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc
     3061 tgccgattcc aaacactcta cgactttttg gatcgtttct tcttctagaa tatctactgt
     3121 ttcttttatc gctacttgtg cattgttacc aagtttgttt ttgatagagt aaaaggactc
     3181 atttgcttct atatcactaa aaccttcatc gattccagtc tgtagttctg ttgaaatcgt
     3241 gattttgaag tccgtcagac tttgaaaacc gatttttttg gaaaaacgta caactgtcgg
     3301 cgcactcaac ccggaagctt ctgctaattc attagctgtc attgtaggga ccttctcaat
     3361 attaccgata atgtagttgg ccaacttttt ttctgctgaa gaataatctt ccatcatact
     3421 ttcaatacga ctaattacgc tcataagttc ctccaccctt ttaatgatag tctatgtaag
     3481 tataccatat cagaccctta ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc
     3541 gcccaacaga atatcttatc ccatcaatcc cacagattct attgagcatc tgtgtctttg
     3601 aggaatacct gttatctatt gttcggattg ctttctatct actacttttt tacaggaaat
     3661 ttacctatta gttttctctg ctgtaatttt tgacttctgc ttccatagca tcaataaaga
     3721 tattcaaatc ttctacagct gtttctttac ttccataacg acgtacgttc actgttgcat
     3781 cttccaattc cttgtctcca acaactaatt ggtaagggat cttttgtgtt tgagaggcac
     3841 gtatcttata gcccattttt tcattacgat catcaacttc tacacgcaac cccttcattt
     3901 gcaaacgttc tttgatttca taagcataat cgccatgtgc atcaacagaa acagggatga
     3961 ttgttgcttg tataggtgcc aaccatgttg gaaaagcgcc tttgtatact tctgttaaat
     4021 aagcgacaaa acgttccatt gtagaaacaa ttccgcggtg gataactact ggacgatgcg
     4081 tattttcccc gtcttctcca acatatgtca aatcaaagcg ctctggcaat aagaagtcta
     4141 attgtatagt tgataatgtt tcttctgtac caagagctgt tttgacttga acatctagtt
     4201 ttggtccgta aaaggctgcc tcgccttctg cttcaaagta atctagttct aattcatcca
     4261 tagcggcttt cagcattgct tgggcttttt cccacattgc gtcatcatca aaatatttat
     4321 ctgtattgtt tggatcgcga tagctcaagc ggaaacgata atctgtaata ttgaagtcag
     4381 catatactgc caccatcaat tcaagcgtac gtttaaattc atctttgatt tgatcaggac
     4441 gaacaaatgt atgaccatca ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag
     4501 cacctgattt ttcatagcga tgcatcattc ctaattcagc gatacgaatt ggtaattcac
     4561 gataagaatg gatcgtgttt ttgtagacca tcatgtgatg agggcagttc attgggcgaa
     4621 gaacaagcat ttcgccatct cccatatcca ttggcgggaa catgtcttct tgataatgat
     4681 cccagtgtcc agatgttttg tacaactcaa catctcccat tattggtgta tatacatgtt
     4741 ggtaacctag actaacttct ttgtctacga tataacgttc gatcgtgcgt cggattgttg
     4801 ccccttttgg aagccagaat ggcaaaccag aacctacttc tggggaaacc atgaacaagt
     4861 caagttcttt tcctagttta cgatggtctc tttcttttgc ttcttcacgg agacggatat
     4921 attctttcaa agctttcttg tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat
     4981 tgtttgagtt tccacgccaa taagcacctg ccactgataa taatttaaat acttgtatac
     5041 ggccagtgga tggaacatgt ggtccacgac acaaatcgat gaattcgcct tgttgataag
     5101 cagtaatcac ttcttcttct ggcaattcac taatcagttc tactttgtac ggatcacttg
     5161 caaaaatttc taatgcttcc tgttttgaaa gaacacggcg ttcgatcggc agattttctt
     5221 tgacgatttt catcatttct gcttcgattg ccggcaaatc ttctgcagtt acttggtttt
     5281 gtccattatc tgtatcgtag taaaaccctg aatcgatagc tggtccaacg ccaaagtgga
     5341 tatttggata gaggcgacgc atggcttggg ccatcaaatg agctgctgaa tgacggacta
     5401 agccaagtgc atcttcatgg tctggtgtga tgatctcaat acttgcatct tcctcgattg
     5461 gacgagtcaa atcaacgagt tttccgttca ctttaccagc taaagctttt tttgctaagc
     5521 tttttgaaat gctttcagca attgccaatg ttgttgttcc agactcaaat tctttgactg
     5581 caccatctgg aaaagtaatt tttatcataa tagtttcctc cttgaaatgt tttgattgat
     5641 cagatcatca agctgaaaaa caaaaaaatc ctagcatctc ttgaaaagag atactaggac
     5701 gaaaagttcg tggttccacc taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg
     5761 gtccgaccgc ccttgcttca acaagggttt cgaaagtggt catcctttgg ttttctcctg
     5821 gaaaagtttc agcctagctt ttcctctctt atcgaagact ctcaaattca gttggctttc
     5881 tcatcaatca attatgtgtt tatttaacca ctaataaata gaaaaatcaa gtgggaaact
     5941 aaacttattt tttccattcc ccatttacac cgtatgttcc ttttttcatc tcattgatga
     6001 tgacatggat gtgttctttt ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt
     6061 tcatcatatt ttctaattgt tccggactgc gaccttctac taattcgaca tgtacgaatg
     6121 gcataatgct ctctcctctt tctgatcgtt cttttttcaa ctcgattcta tcatactatg
     6181 tttgtcttgg gtactgaatc aggaaaaatg gctggatctt tttttgctct caaggcactc
     6241 aaaatagaga ccgtatccac gacttcttgc aaaacagctc cgaataatgc ggggattagt
     6301 cccgtacttg caattaacat caaaaaaata caaataaaga tgccaatcag tacagactgt
     6361 ttcgctattc tcatcgtatc tcttgaaatc actactgctt ttgctacacg caagagatca
     6421 tctttcaaga tgaccgcatc tgcactttca ctagcagctg tagaaccatg agcccccatt
     6481 gcaataccga tatctgcagc agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc
     6541 gcaacaggtc tttgatcttt agggatactt tctaaaatgg tcagtttatc ttgaggcaag
     6601 ctacgggcat gtacagaatc taattggact ttttctgcaa tcgtttgagc aatggcagat
     6661 ccatccccgg tgatcatcat caaatttttc acatcaagtt ttcttagctc cttgattgtc
     6721 tttgctgctt ctgggcgaat ctgatccgaa aataggatcg ttcctgcaaa agatcgatca
     6781 attgttacat agattgctgt attggacgtt tctatttctt cagctgaaac aaaagaggca
     6841 cgtccaatct ttacttcatg attattgact actgcttgga ttccttgacc ggtcacttct
     6901 ttcagttgac taacaggata cagttggctg tttttcacgt tgttcactaa cgaacgagct
     6961 aagatatggc tagattcctg ttctgcacta gctgcataca gaagtagctc ttcttttttt
     7021 attccattga ctggctgaat cgcctctact tccaatatcc ctttggtaag cgttcctgtt
     7081 ttatcaaaag caatcgtttt tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca
     7141 acgatcccgt ttttacttga acggctcatt cctgcaacta aagcaatggg cgcagctaga
     7201 atcagtggac aaggtgatgc cactaccagt acctcagcaa atcgtaaagg gtcttttgag
     7261 aaccaccaag aaataccacc gatcaaataa gcaatcaacg tgaaagggac tgcataacga
     7321 tcagctaaac ggacaaatcg ggcaggcttt tcttttgatt cttcgactag tttaataatc
     7381 atttgatact ggctgtctgc ggcacgtttt tcagcgatca tttggatcga tccgtctcca
     7441 tttaaagacc ctgacataat ctgatctcca ggtttcttgg tttccgggcg ggactcacct
     7501 gtcaaagaag attcatcgac cgaagattct cctttggtca ctcgaccatc tgcgggtaca
     7561 agttcgccag gcttgactag taactggtca tcaatattta tttcatcgat tactatatcc
     7621 tgcaattttc cttctttgaa tcgatgggca attcttgggg tgttatctaa caaagactgc
     7681 agttctttac ccgctcgttt attcgcgtaa tcctctagac tatctccgcc cgtaagcatg
     7741 atcaatatca tcaaactagc ccagtattct ttgacggcta atgttgcaag gattgcggta
     7801 atcgctaata catctacgcc atatcttcct gaacgtaacg tcttgatcat ttccaaaaac
     7861 atcaagaagg cgagaatccc tccagtaaat gcgacaatcg caaatgcaag ggccggctgg
     7921 tgaaaaacaa attcacttaa taaagctatc ccaccaataa gcaaagtaag taaaaatttt
     7981 ttgaaatgtg tcattgtttt ttctcctctt ttatgtgact tagagaggtt agtaataaat
     8041 ttccagctgc ctctttcatc ttaagcatag tccttgacta gcacttttta aaacaatagg
     8101 ccttaattga aaacaactat cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa
     8161 aaatattcag caaggattac tttttcgatt catcagttaa aaagtacctt ttttcgaaaa
     8221 actatgctat acttttaatt cgttatttac ggcggaagga gcaaacatgc gatgaatgag
     8281 aaacaactag aacaaacgca cgtggatgaa acgatcgaac taatccagat cgaacaaaat
     8341 atgctggata caaaacaaca agaattgact caggaaatga atcattcgac aaaagacaca
     8401 gctaatcaca agattcgagc tggttccaat gaagcttttt atgaatctgc tgttgaatat
     8461 cgccaacatg agcaggaatt gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc
     8521 ctgcaaacgt tgtctgtcat gaaagatagt ccttattttg cccgtatcga ctttaaagaa
     8581 gaccgtgaaa cagaaacatt atatctaggt atcgcttctt taagagatct ggcagaagaa
     8641 acaatcgtta ttgactggcg cgcaccaatt gccaatcttt attatgaagg tgagattggt
     8701 cccgcatttt atgaaactga aacagataga atagatgtcg atctgcagtt gaaacgacaa
     8761 tttaaaatcc aagaaggacg tattttatct atggtagata cgtcggaagt aatcaatgat
     8821 gactttttgc tagagatatt ggatgaatca tcaagcaacc atatgaaaaa catcgtctct
     8881 acgatacaaa aagcacaaaa tgcaatcatt cgggacacaa caagtaaagt aatgctgatt
     8941 gaaggaatcg ccggaagtgg aaagacatcc gctttgcttc aacgtattgc ttttctgttg
     9001 tatcataatc gcaagtggct cgaggctgaa aacgttcttc tattttcacc caaccatttg
     9061 ttttctgatt acatctcgac tgtattgcct tcattaggtg aaagcggcgt gcctacacag
     9121 acattcaaaa attatttggt gcagctgctg ccagagtttt ctttactaga tgaacagcaa
     9181 caagaaagtg gttttttgct aggggaaaaa gatccgatac aagtgatgaa aagcggattg
     9241 accttagtcg atcagattga tcgctatatc caaggcatca caaactacgg tcccttgttc
     9301 cgtgatatga aaataaatgg ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat
     9361 aaagaaacaa atgaacaatt acctttacat catagacttt ctctattaca aacgaagctc
     9421 ctgaaaaaat taggcggtct gcaaaaagac gaaacacgtc agcaatgggt caaagatctg
     9481 gcggaagaac agctccaaga actatacgca acagatccaa atcttgaata tacagaacaa
     9541 aaggagcgtt cattacgtaa aaaactggcc aaccaaatcg tacgtaaaag attccgtcga
     9601 atccatcgag ggatcatgaa ttatcaattt gtcaatataa cgaaacaata tctccacttt
     9661 ctacaaactg tgccactttc tcttctaaca cgacataaaa tcagcgagac caggtggcag
     9721 gaacatttga caactgttcg tacacacttg cgtcaaagag aattgcgtca agaggatgct
     9781 gtactattct ttttactgat gcgccgaaca tatccagtga cggttacaca caaagctcgc
     9841 tatatcttta ttgatgaaat gcaggatttc gcaccatcac aagtggcatt gctgcaagcc
     9901 ttgttcccaa aagcaaattt gactttctgt ggagatctaa atcaaaatgt ctttggaaat
     9961 gaaacgatcg ttggatcatt ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa
    10021 ttaacgacaa gctatcgctc cacaaaagaa atcactgatt ttgctaatca ttttattgtg
    10081 aaagaacatc aagtagaaac aacagcaaga aaaggagacc ttcctttgct catccaagga
    10141 acaacagatg atatcaaatt gaattggtta gcagatacca ttcgaacgac tcagggaaaa
    10201 gcccggtatt ggcgtacagc aatcatagct aaaacacaag aagaatgcga agctctttat
    10261 gctcaactgc cagacgagtt gaaacaacaa gtccagctga ttttaaatga gagtgacttt
    10321 atgaagcgtt ccattatttt acttccagcc tttttagcaa aaggtctgga gtttgaccga
    10381 gttttcttgt gggatgtaga tgcagaacat ttttcttctc agcaagacaa attgatcctt
    10441 tacaccatgt gtacacgtgc gatgcatgaa ttgacccttt ttacctccaa taatgaaagt
    10501 cctttgttaa acaatatgga tatttcatct tatgagaaaa aagtactgta agtaaaaaga
    10561 caactgctat cattaatgtt agcagttgtc tttttattta caataataat tgcagaaaaa
    10621 gtaattcaat catttaaggt catttattct atatataaat ccaaatctgt ttcaggatat
    10681 tttaatttga caagcgaaat aatcattttc agttcttcca tgttttctgg atattctggg
    10741 tcaaatacct tgctatgacg gccaatcccg ccattgtcga tcaatcggta aattcgattc
    10801 cattcaatga attggtcaat tgattggaaa ttgctgattg gatagccgcc acaatccaaa
    10861 atctctgata cacgaaatgc taggtatgca ctatcgatca ctttattttt tttcgtgtct
    10921 atcttctcat ttctttcgat aagaattttt tcaggacgat gtttcaagct ccactctaat
    10981 agtagaaaga aagcctccat ccgatactct tcttcaaata accgaaaggc tgaatcagaa
    11041 cgacgaaaaa gcagcggaat cagataattt tgtctataaa tcagcattgg tgcgcggtcc
    11101 accatacact ctaaataacg gttcagatac cctgtaccga catagtttcc taatcgatag
    11161 ccttcaacgc cgaatcttgc gtatttttgc gctaattttg aacaatcagt tacgttcaca
    11221 gctaactctt ctactttgcg gtaaccgctt aagatacgtt caaaaggttc taaagtgatt
    11281 tttgcttcca tattcttccc cgtccatctt ccacatactt catatcattt cacatgatac
    11341 ttatgctctc atcataccac aggttgaatt agaaaagaaa ataaatcaca caaaaaagca
    11401 agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa
    11461 actggttgct tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc
    11521 ttccagtgca tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc
    11581 gttttgaggg atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa
    11641 tgcttcttgt atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc
    11701 ttccattaca tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc
    11761 gtcttctcct gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga
    11821 aacacccatg ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt
    11881 cacattgttc gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata
    11941 gcgcaattca gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat
    12001 aatatgtttt ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt
    12061 tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc
    12121 ataaatccgg ctattattag catctttagc agttttcttt gctacgatat ttgcccattt
    12181 acgtcccata ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg
    12241 ttccattata cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt
    12301 ctcattttta gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa
    12361 tattgcctcc caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct
    12421 ctttttttcc gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc
    12481 cagcatcggt aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt
    12541 gctttttgat attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc
    12601 tttttgtttt tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt
    12661 tcatgcgcga ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag
    12721 aagataacaa ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag
    12781 ttgaaagatc ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta
    12841 acgcatggcg aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat
    12901 agcctgcact tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt
    12961 cctttgcata acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg
    13021 gatacagact gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca
    13081 gttcttgaaa tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc
    13141 tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt
    13201 gcaagatttt tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg
    13261 gaagctccac tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat
    13321 cgatgatagc cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct
    13381 ctgctcctgt tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat
    13441 tgtactctac gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa
    13501 aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa
    13561 ctattctcgc aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat
    13621 atcgttcgga tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc
    13681 aaaaccgcta gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt
    13741 cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta
    13801 aataactttc caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac
    13861 cagacccttc ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc
    13921 ttcctggata actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt
    13981 ctggaaaaac ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt
    14041 cttctaacgc ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat
    14101 agcacagcga atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga
    14161 gcatattgcc ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa
    14221 tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg
    14281 caaccgctaa tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta
    14341 aatgacgttt cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt
    14401 aaatgaaagc aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta
    14461 gcggagcatc tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa
    14521 catgtacgat cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa
    14581 tacgttttac ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt
    14641 tgatttgacg ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat
    14701 ttcgaacgtc taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa
    14761 tttttaattg ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt
    14821 tgctacccag taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag
    14881 caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc
    14941 aatggctcgt gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac
    15001 ataaatgatt ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc
    15061 atgaagaatc tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag
    15121 accatgccag attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat
    15181 taccattttg aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat
    15241 actagctttt tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac
    15301 tccaagcaca tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt
    15361 ttctctagaa taagcagtat attcattact atagtccata ttcatacaac ccttcatttt
    15421 gaatataatc aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct
    15481 tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat
    15541 atggagtatc cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca
    15601 gctcatcgat tttataccat tttggcaaat attctaccat atccccaccg ataataaagt
    15661 aataatctgt atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt
    15721 tcccaccacg agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta
    15781 attccaacat gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg
    15841 gctgataaga aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag
    15901 ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct
    15961 ttttcttctc taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct
    16021 tagcttgcgt attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt
    16081 gatacttttc ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga
    16141 tttcgcaatc tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat
    16201 tttgaagtaa cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga
    16261 tcattgctga attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt
    16321 tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa
    16381 tcaatgcttt gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg
    16441 gcgctgttac agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag
    16501 aaaactcaaa acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta
    16561 ataatccacc tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa
    16621 tatttacatc atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa
    16681 atcttgcaag tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt
    16741 tgatttcttt taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt
    16801 gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct
    16861 cgattttatc caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg
    16921 ctgtattttt gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga
    16981 tatagacatc ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat
    17041 gagatttctt agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac
    17101 gttccttcat ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt
    17161 tgttcccaac taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg
    17221 aaccattgaa gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt
    17281 cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac atcttgggtc tcattataat
    17341 ggcgtaatct gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag
    17401 ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc
    17461 cgatacattg taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt
    17521 tgctaataga taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact
    17581 atctgttgcg ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg
    17641 tatatcagtc ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg
    17701 tttctttgct atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag
    17761 atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac
    17821 agggattcct gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg
    17881 attattccat gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt
    17941 ctttaattgg gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt
    18001 tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac
    18061 cccgtattat actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa
    18121 aaagcatttc tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca
    18181 aaaaacaact aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct
    18241 aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta
    18301 tatcgtggca actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac
    18361 tcgcgtagtt ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg
    18421 tctttccaca aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc
    18481 agcatccaga cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca
    18541 atgatgatgg cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata
    18601 gcctctcctt cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc
    18661 aaggtaatca ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga
    18721 tagccttcag gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct
    18781 ttttgaatac cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc
    18841 gcttcgtcat ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct
    18901 tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt
    18961 gctgtcttat ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat
    19021 tttctgtttc aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa
    19081 ttcagccttt tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct
    19141 tccagcaaaa ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa
    19201 actggcagtt actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc
    19261 attgctatgc cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc
    19321 ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt
    19381 tgctcgttcg aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat
    19441 aaaattagga tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt
    19501 gaggactgct tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc
    19561 gggaaatgct agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc
    19621 atcaataatc aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg
    19681 gcatactttt tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt
    19741 tgcccacatg ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt
    19801 gatccgaata tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc
    19861 tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat
    19921 gactgcagga tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt
    19981 gataaattca cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg
    20041 gacgattatt tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac
    20101 tgcactgtct actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc
    20161 tggagaagga gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg
    20221 tccttggaat actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac
    20281 ctgttttttt actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat
    20341 tcgagtattc atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt
    20401 tccggcattt tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc
    20461 tctttttgtt tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg
    20521 ttgtaaagaa caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat
    20581 ctgaacttct atatggcgag caggataaat gattttttcc aaatacatat catcatttcc
    20641 aaatgccgct gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga
    20701 ttgaactttc cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata
    20761 accaattctg tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc
    20821 tggaatgact gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat
    20881 tgcatcgatc gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga
    20941 ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt
    21001 cacgattgca gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg
    21061 gccaatgcag attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga
    21121 atatatcgct acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat
    21181 ttcaccgcgg ttagctatca atactttaga aaacatattt cacctatcca atcataaagg
    21241 ttaattccgc ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg
    21301 ctgaagcttt cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt
    21361 tcttacggaa ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt
    21421 caagagaaag taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg
    21481 gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt
    21541 tcttagcaac gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg
    21601 gataacgatg tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct
    21661 gctttgctta tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt
    21721 tttctacaag gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt
    21781 tcatcgcttc aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga
    21841 aagctggttt gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct
    21901 cgattccatc aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt
    21961 gttcaggtat gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat
    22021 tcatgcctcg acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat
    22081 cgaattccgt tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat
    22141 ccaaaattat tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa
    22201 tgaattactc attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa
    22261 gtcaatctca ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa
    22321 tgccagcaaa gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat
    22381 cgatttcgtg ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc
    22441 ttttgcttct gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac
    22501 ttgttccggc tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga
    22561 accatctgga gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat
    22621 ttctccatag attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac
    22681 gcctgctcct tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg
    22741 attaggatca gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc
    22801 acaaatcgtt gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt
    22861 acggaatgct tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca
    22921 aatacctttt gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat
    22981 tggtacgaac attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt
    23041 ttcgattgtt ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt
    23101 cattttttct ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata
    23161 gatcgaaaac atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa
    23221 atctttgact tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg
    23281 tgcgatgcca ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat
    23341 aggtgaagtg actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc
    23401 ttatccgtgc atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt
    23461 ttgtgctaag aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg
    23521 aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt
    23581 gatgaatcct ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac
    23641 agattttgtt aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt
    23701 tccaatcaac ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat
    23761 ttttttcaat acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt
    23821 tttgaaatct tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt
    23881 tactaacaca tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt
    23941 ctcataatca gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc
    24001 ttctatcttt tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt
    24061 tgcaaaagct aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt
    24121 ttttcctttt acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc
    24181 tgcttattct caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt
    24241 aatacttttc cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg
    24301 atactctcat agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt
    24361 tctttcttca ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat
    24421 tgaatctgac ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga
    24481 aaaggcccgc tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa
    24541 acagcttcgt ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa
    24601 tttgcaggag atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca
    24661 atcggcgcat tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata
    24721 taagcgcctc tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct
    24781 actaatgccg aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc
    24841 tccttcaata agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc
    24901 gtctgatcca aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc
    24961 tcacttgctt catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata
    25021 ccttgatatt gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat
    25081 atatccagaa tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag
    25141 gaaatgtccc agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt
    25201 cactaaatct tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc
    25261 ttcaatttca ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat
    25321 gtcagtagtc aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg
    25381 aattttttcg aataccataa taatatattc ctccgatatt gtttgctttt tatttttata
    25441 atttaacagt ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg
    25501 taaatattac tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca
    25561 ataagatagg gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact
    25621 tttcttgcgg catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat
    25681 gcaaaagcag ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt
    25741 ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg
    25801 aatcttctga atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc
    25861 tggtaccgtc agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga
    25921 gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta
    25981 gcttagatag tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt
    26041 tttcagccat gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga
    26101 tagcatgagt tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat
    26161 cgggcgacat tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt
    26221 ttatcaattt ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc
    26281 ttcgttcacg aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg
    26341 aaagatcatt attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc
    26401 caaattgttc cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca
    26461 gtgcatccag caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca
    26521 tttcacgatg aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca
    26581 actttacaac gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta
    26641 acttgttaat agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg
    26701 aagttgtttt tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca
    26761 gatctttgaa ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga
    26821 cactaacaag ataatcattt actgttttta aatctggttc catgttccac cgtccactaa
    26881 ttagtttgat tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatg
//
LOCUS       DAFGFZ010000029        26425 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000029 DAFGFZ010000000
VERSION     DAFGFZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.029"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            56..352
                     /locus_tag="LR726_001064"
     CDS             56..352
                     /locus_tag="LR726_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL6216170.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(996..1949)
                     /locus_tag="LR726_001065"
     CDS             complement(996..1949)
                     /locus_tag="LR726_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6216171.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1972..2727)
                     /locus_tag="LR726_001066"
     CDS             complement(1972..2727)
                     /locus_tag="LR726_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6216172.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2724..3683)
                     /locus_tag="LR726_001067"
     CDS             complement(2724..3683)
                     /locus_tag="LR726_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL6216173.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3676..4626)
                     /locus_tag="LR726_001068"
     CDS             complement(3676..4626)
                     /locus_tag="LR726_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL6216174.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4776..5228
                     /locus_tag="LR726_001069"
     CDS             4776..5228
                     /locus_tag="LR726_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL6216175.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5317..7584)
                     /gene="gshAB"
                     /locus_tag="LR726_001070"
     CDS             complement(5317..7584)
                     /gene="gshAB"
                     /locus_tag="LR726_001070"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL6216176.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7771..8769)
                     /gene="ftsY"
                     /locus_tag="LR726_001071"
     CDS             complement(7771..8769)
                     /gene="ftsY"
                     /locus_tag="LR726_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL6216177.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8780..9589)
                     /locus_tag="LR726_001072"
     CDS             complement(8780..9589)
                     /locus_tag="LR726_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL6216178.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9598..13179)
                     /gene="smc"
                     /locus_tag="LR726_001073"
     CDS             complement(9598..13179)
                     /gene="smc"
                     /locus_tag="LR726_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL6216179.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13199..13885)
                     /locus_tag="LR726_001074"
     CDS             complement(13199..13885)
                     /locus_tag="LR726_001074"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL6216180.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14233..16014)
                     /locus_tag="LR726_001075"
     CDS             complement(14233..16014)
                     /locus_tag="LR726_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6216181.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16040..16954)
                     /locus_tag="LR726_001076"
     CDS             complement(16040..16954)
                     /locus_tag="LR726_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216182.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16981..17943)
                     /locus_tag="LR726_001077"
     CDS             complement(16981..17943)
                     /locus_tag="LR726_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216183.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17943..18887)
                     /locus_tag="LR726_001078"
     CDS             complement(17943..18887)
                     /locus_tag="LR726_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216184.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18888..19892)
                     /locus_tag="LR726_001079"
     CDS             complement(18888..19892)
                     /locus_tag="LR726_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216185.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20160..20402)
                     /gene="acpP"
                     /locus_tag="LR726_001080"
     CDS             complement(20160..20402)
                     /gene="acpP"
                     /locus_tag="LR726_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL6216186.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20524..21525)
                     /gene="plsX"
                     /locus_tag="LR726_001081"
     CDS             complement(20524..21525)
                     /gene="plsX"
                     /locus_tag="LR726_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL6216187.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21574..23610)
                     /gene="recG"
                     /locus_tag="LR726_001082"
     CDS             complement(21574..23610)
                     /gene="recG"
                     /locus_tag="LR726_001082"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL6216188.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23727..25409)
                     /locus_tag="LR726_001083"
     CDS             complement(23727..25409)
                     /locus_tag="LR726_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL6216189.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25461..25823)
                     /locus_tag="LR726_001084"
     CDS             complement(25461..25823)
                     /locus_tag="LR726_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL6216190.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26093..26281
                     /locus_tag="LR726_001085"
     CDS             26093..26281
                     /locus_tag="LR726_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL6216191.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7280 a         5734 c         4662 g         8749 t
ORIGIN      
        1 caactcttcg gtagagtaaa cttatcaaat aaaagacttc tttggaggaa tcactatgaa
       61 ggaatatata aaaaacatct attttatcga agaaacacaa aatatcgagg gttcttatat
      121 tgaagtaaaa acactctttg tcaatgaaga taaaaccaaa gcattggata tttataaaaa
      181 attagcttca aaaaaaacga atagttttgg attgatatta agcgagtata aaatcaaagc
      241 agaagagagt tatttctatc aattactaaa acggtggagt aagcttcccg ccgattttta
      301 tcgaaaaatg cagatcatca actatcaacc gcttgctgag acacatgcct aaatagaggt
      361 tgtgaaatca gtgctttgag ctaataccga aggtcagctg attgaacatt gtttagaaga
      421 gattgtgaca actgcgattt gctttgagta ataagaacgc tggacagaaa atagcttctc
      481 atattttcta gtctagtggg cttattgccg agaagcagca gatggaacat cgtttagaag
      541 agattgtgac aactgcgatt tgttttgagt aataagaacg ctggacagaa aatagcttct
      601 catattttct agtctagtgg acttattgcc gagaagcagc agatggaaca tcgtttaaag
      661 aagagatcgg tacaaaataa aaatatctaa atttaagttt tatctaagtt tcgctttgta
      721 cctttgattc aaatcaaagg aggcgatctt gatgctgaaa aaatggatca cactattttt
      781 tgccactccc cattctagaa tcaacccgta tgatgtagat aatgaattca cgttgaaaaa
      841 agatttctca aataaataaa aaccatccgt aaaatcatca atacgatttt acggatggtt
      901 tttgataact tctttgagga taggctcttg ctatttaaga atgtaagaaa cattattgtt
      961 acatagtatg agagtttaat cctctgttat ttatgctatt taaatgcttg attcacatct
     1021 tcgatcatca gtttcattga ttctaaaccg cccccgctta aataccagac atctggttca
     1081 agggagatga tctgctgatt tttaccagca ttcgtttgtg ctaccagttc attggcagaa
     1141 atatcatctt tgctgtcgtc tccaccaata gcttttgttc tgtctactac aaataagata
     1201 tccggatttt tttccagcac atattcataa gaaacacttt gaccgtgagt ggatgcttcg
     1261 atttgatcat ctgcttgttc aaagccaaat aaatcatgga tgaaaccgaa tctagaacca
     1321 gatccgtagg ctgataattg cccttcattc acaagtgtga ctagtgcttt tttgtcagta
     1381 gcttctgctt tctttttcgt ctgatcgatt gcactagata attcctctaa ttgagtttta
     1441 gcttcttctt gcttaccaaa gatctcccct aaagcagtga cattttgctg gaaagattcc
     1501 catggatttt tcgcatctaa agaaaggaaa atggtcggag caatttgact tagatctttt
     1561 tgaaaatctc tttgtcggcc agaaatgatg attagatctg gctgaagctg attaattttt
     1621 tctaagtctg gttcttttat tccacctgct gattctactt ttttatagga tgaaagatac
     1681 tcaggaaggc tgcttgtagc tgctccaact accttatctc caacaccaag agcatccatc
     1741 gtatctaaag aaccattgtc gaatacgacg actttttctg gattatttgg cacctcgatt
     1801 tggtctccat tactatctgt tatcgtcaat gtagtttcag tttgtgtgct gctttttttt
     1861 gtgtctgcct gactggtgtt gttgctattt ccacatgctg ctaaaccaaa tgatgctaga
     1921 atcaagatac ctactactaa ttttttcatc tcttctctcc taatgttagt tttagaaata
     1981 aagacagaat ctgcggcctt caatctcaca aatccgtatg tccatttcgt ataattcatc
     2041 caatacatct ttttttatca tttcttgggt cgttccttgc gtaaataact gtccatcttt
     2101 cattgcaaca atttcgtctg cataacttgc agcaaagttg atatcatgaa gaacaataac
     2161 aattgttttc ccgatttctt gtactaaatt ttttaaaatc tgcatgattt gaaccgcaaa
     2221 attcatatct agattgttca aaggttcatc cagtaaaata taatccgtgt cttgcgctag
     2281 caccattgca atatacgcac gttggagttg accgcctgaa agagtgtcaa tcgattgatc
     2341 cgctatttct tctaacccaa tgtgttccag tgattcctga acgatttcct cgtcttttgt
     2401 cgtcaatttt cctttcgtat aaggaaatcg tccaaattct actaattctt tcacggtgat
     2461 ttggacattt atttcattcg attgtcgtag gatcgataat tttttggcta attcattgga
     2521 tttccaagcc tttacttcgt tatggtctaa atagatttca cctgtatctt ttggtatcaa
     2581 acgactgatc atcgacaaca ccgttgactt tccagcacca tttggaccga taaaagcagt
     2641 caacttcttt tcaggaatcg ttaactgtac atctttaacc actgattttc catggtacag
     2701 tttattgacc ttttgtaaat caatcatagg atactcttcc tttcgcgaat caattttgtg
     2761 acaaaataaa ttcctccgac aaattcaacg acgacactca ctgttgtatt ccagccgaaa
     2821 atctgctcta ctgcgagctg tcctcctgtc agaaacagca tagctaccag aaagcttccg
     2881 ataaatagct gaccatgacg ataagtagaa aagacacgat aactgatatt tgcgacaata
     2941 aaccctaaaa aagtaactgg acccacaaga gccgttgatg tccctgtcaa aagactgaca
     3001 caacataaaa tcaaaaattc aaaacgatct gtgtctatcc cgaggtttac tgcttgttca
     3061 tttccgagat ggaaaacgtc tagcacatgt cgatatcgaa ataacaggta ccagcagccg
     3121 ataatgatca aagaagcagg caaaagatac tgactctgga tattgccaaa gcttgcaaat
     3181 aatctgcctt gaagcaaatc atattcattc ggatccatga tgacttgtaa aaaggtactg
     3241 acactaccaa aaagcgtacc taaaatcatc cctgacatca gtaataaaaa aagattcgct
     3301 ctcatttttt tcaaaagaaa gaaaaataac aatagactca gcccgaccat cagcccaacg
     3361 ttcaataaga aaaaaactgt acctgtctgc tgaagcatcg tcactcctcc gataaagaag
     3421 aaaaggaccg tttggacaag gacatataac gactcaagtc ccaatactga aggagtcaaa
     3481 aactggttat gcgtaattgt ctgaaagctg attgtggcac tgctgacagc tagcgagaca
     3541 aggataaatg caaaaatttt cttactcctc aatgcacatg caaaatgcca gtttccatat
     3601 gtatggatcg tcaagtacat tcctgtaaca aataaaacaa gaacaagcat accaataaaa
     3661 atagatggct tttttttaag catatcgtcg tccccttatc agaagataaa ggaagatagc
     3721 cccaccgatg attccaacga tcacactgac tggtatttca taaggaaaaa tcaacacgcg
     3781 agcgacaaga tcgcaaaata ctaagaacaa actacctgct aaagcagtta cgccaattgt
     3841 tttgtgcact tgatctccat atattctaga gacaagattc ggaatgatca ctccgataaa
     3901 aggaatgctt cctaccatga tcaacatgac tgcactagcc aatgatacta agccaatgcc
     3961 taaaaactgc atcttctggt aatctattcc cagatttact gcaaacgatt cgcccagacc
     4021 aatgatcgta aatcggtaag caaatagcca aaccaagagc aacaatggaa caacagcata
     4081 caataactca taatttcctc gtatcacagt cgcaaaattc ccttgaagcc atgaagtcat
     4141 attttgtacc aactggaatt gataagcaaa aaaagtagca actgaaccaa tgatattccc
     4201 taacatgact ccaatcaacg gaagcatcaa ttgatttttc tggaggaaca atcgcgtaag
     4261 ggataaaaaa agcaatgttc ctgcaaaagc aaagcaaaat gctgtcaagg atcggacaat
     4321 caaggatgca ccaggtagga aaatcatagc cactaaaata cctaaacgcg cactatccat
     4381 cgtgcccgca gtcgttggtg aaacaaattt attttgggtc aaatgttgca tgatcaaccc
     4441 acaaatacta ctggtcgctc ccacaaggat caaacaaatc gttcgtggca aacgagtcac
     4501 tagcaagatt tgtaagcttt gcccatctaa ctggagcagt tctgacaatt gtacattcga
     4561 tactccgatg aacaatgaaa tactacttaa aataactaat aatgctacta gtcgtccgta
     4621 gcgcatccaa tccacctttc taattgagaa tgattttcat tcttatttta agtgaatcgt
     4681 tgaacaaaag caaccgcttt ttcatatgtt ttaggtatta ttgggaatat gctataatcg
     4741 taagaaagac aaagagaaga aggagggtat tttttatggc acaagatgat gtaaaaagcc
     4801 atttggataa gggactttat ggtacacctt tagtgaaccc tgaagaacag cataaatatt
     4861 tagggacatt cagggaacgc tgctatctta gtatgactgt tgctgaaatg atccaaccaa
     4921 aaaacaaaga aaacttttta aaagaactca aaaaacatcc agatgcttcc atactcttga
     4981 atggggctct acccctttct gtccaaaacc aatatatcca gctagcaacg aaaaacaata
     5041 gccgtttcac tgtagtcaac gattacgtca ctgcaaatcc tgaggaattc ggattgctct
     5101 taacagcaaa agaagcagta aatgaacctg tgattgatat cgaaaaaaaa tacccagaag
     5161 aaaactcttc gccagctgaa aaagaaaaac caaaaaagaa aaaaagcttc tgggatcgtt
     5221 tattttaaaa cgaaaaaaca cataaatttt tacatgcaaa aaaagctttt gatcagattg
     5281 acatcaaata ggacaatctg tcaaaagctt tttcttttaa aatacttcag gatataacaa
     5341 tttcaataca ttcatcgtca atcttctgcc ttttcctgca aaaggataaa catgcatatg
     5401 catggctgga ttgaaatttg cttcgatgat cccatatgct tttttatcag ttgtaggatc
     5461 gatttcttta tctggtataa tcaaatcgat accactaatc ttagctccta gtgcttctac
     5521 cgcagaaacg gcgatttttt tgtaagcttc agaaaattca tcagtcatat cgatcgaatc
     5581 accacctgta ctgatatttg agttctccct tagataaaca atttgattgg cttggggaac
     5641 tgattcgatc gtcaaccctt gttctttcaa catcaattgt tctaattcac ctaattggat
     5701 caattctaaa ggtgcacgat gattcgttcc acgcaaaggg tcactatttt tttcttcaac
     5761 aagttctttc actgttcgga tactgtcacc aataacatta gcagggactc gcaacatgat
     5821 ggcttggact tctccgtcga tcacaaagaa acgatattcc gttcccggca taaattcttc
     5881 tactaaaacc gaactgtctt cacgaaaagc aatggctaaa cctgcttgat aatcttccag
     5941 actagctcct tccttgaaaa tcgtgatacc taacccgtaa ttggttgatt taggtttgat
     6001 cacaaatgcc tgctcggcaa atctcggata agcttttacc gcttcttcca tggatgaaaa
     6061 ttctgcgcct cctggtaccc ggaacccagc ttcttttaag acttttttcg ttactgtttt
     6121 gttttccatg ataagtggga cgatatagct gtccttactt gtcatgtttg catttttcac
     6181 atattctaca tggtcttgga attgcagacg caaaaattga tccgactcat ctaatacttt
     6241 gacttggaca cctttttgga tggcatcgaa cataaatatc tgagtagaaa gttccatttc
     6301 gcgaaaaccg gccaactgat agggacgttc atggctttct ttataatatt gatgtcccaa
     6361 tgccacagca acttcttgct gactcgatag ctgtgcttct tgatagagtt tcgctgctaa
     6421 cgtttgttca ggatgatcca tttgagtcaa tgcttcctct actaaataaa gagaatccaa
     6481 ccaatccaat tcaaccagca tttgcttcat tccttcaaga atttctctac cttcttgatg
     6541 aaattctgtt tgatctgatg gttgttctag tgctactttg ttatttcgag tcgtaccttc
     6601 tgctacccat tggtcacagt cttctttttc ttctgtccat aacatgtaca tcaggaataa
     6661 ttgtaagaac cgaacttgct ctggattgat acccaatctt gcataaggat tcaaatcgat
     6721 attccgcaat tcaatataac gaacacctgt tgttgctaaa tctgcgactt tctttccgcc
     6781 tcggaaacga agcggtgtat aaaattcttt ctcctccgaa agtttccctt cttcgatcat
     6841 tcgctcgata tctgctaaat actgtttcat agaagcatag gaaactttca catttggatg
     6901 attcgtatag cccagcgcac tgttgcgaat actgcgaaca ggttcttgcg gatgtgattc
     6961 atctaaaaag tagttcttct cactcattgg tgaagcaccg aataaataag tgatcatcca
     7021 gcgatagcgc ataaagtttc tcgctgtttt caaataaagt tctgttttga attcggaaaa
     7081 atcttgaaat tcttcctgat ggctgaacaa tgttcttagc aactcatcgc caaattcaaa
     7141 attgaaatga ataccgctta ccatctgttt tcgcttcccg tattcttttg ctaaataacg
     7201 tcgatacaag acatcttcga aattttttaa ttttgcaata atgatctctt cgtctttttc
     7261 cggtaaggca ggtggcatac ttaatggcca gatcatctct tctttcggta tagaacgagc
     7321 agtcacatca taaacagcag cgagatactg aaataattcg ggaatagaat cggtaacagg
     7381 ggtgatcatc tctgtttgtg tttcgctaaa atctgtttgg atataggggt gataggatcg
     7441 atcgccaaag attgctggat gatcggtttt tgctagattt cctgcaagat caacccgctg
     7501 tccttcccgt tctataccaa atcgagcttg gtcgataaaa gggcgcgcat tgacatgcaa
     7561 taataattgc ttaaaattca tcattaagtg acctccaatt catatgaatc taattataat
     7621 caaaaagtct aagaaaaaga acgaaattgt ttgagaaccg cttacaaata agtataaaaa
     7681 tgaaggcatc aaaaacacag ctatgttaaa aacaatcatt caaacaagga gctgcaactt
     7741 ttgtcacagc tcctgctttt tagacatggg ttattcttct tttagtaatc ctttgaacaa
     7801 tccaacgaca aaatcatttg gatcaaacgg ctccaagtca tcgattcctt caccaagacc
     7861 gaccaatttc actggtaaat gcaattcatt acggatagca agtacgatac cgccctttgc
     7921 tgtaccatct aattttgtca aaacaagacc ggtcacatct gttgtttctt tgaattgttt
     7981 tgcttgaacc atcgcgtttt gaccagtggt tgcatctaat acaagaagtg tttcttgggg
     8041 tgcttctggt aattcccgct ggattactcg ttttattttt tctaattcgt tcatcaagtt
     8101 cactttattt tgcagtcggc ctgcagtatc aactagtaaa acatctgcct gctctgtctt
     8161 tgctctttcc aaagcatcga atacgacagc tgctggatcg cctcctgcat tgccacgaac
     8221 aacttcaaca ccagcgcgtt cgccccagac aactaactgg tcgattgctc cagcgcggaa
     8281 ggtatctgct gctgccatca atactttttt accttcgttt ttgaactgat gggcaagttt
     8341 accaatactt gttgttttcc ctacaccatt aacaccgaca aacaaaataa ctgttagtcc
     8401 gtcttcttgg atatttagtt catttacttc attgattcct tccgcttcat agagatccac
     8461 aagtttctcg atgatcgcat tttggacttc tgccgatttt ttcgcatttc tgagtttcac
     8521 ttcttgacgt aatgcttcgg tgattcgtat cgctgtttca aaacctacat ctgcaccgat
     8581 aagtgtttct tctaattctt caaaaaagtc ttcatcgact gttcgaaaat tggcaaacaa
     8641 ctcattcatc cgttcaccaa atgttttacg cgtcttttct aaacccttgt cgtacttctc
     8701 ctgtacttca acttcttctg ttttctcttc gccagtaaaa gcacgcttga ttttatcaaa
     8761 aaatcccatt tgactccccc tactctaata caaatttttg gatcactttg gctacgccat
     8821 tttcttcatt gctggctgtc acgaaatctg ctgcttcttt gacgaaaggc acagcattct
     8881 ccatcgctac gcctagtcca gcatattgga tcatcggtaa atcattttcc tcatcaccga
     8941 tcgccatgat ttcttcttgt ttcaaaccaa gatcttttgc caataaagaa ataccataag
     9001 cttttgtaac accttttggc ataaattcca aaagcatact tcttgttttg atgatctcat
     9061 acatttcatg gaattctgct ggtatttctt tgattttagg atctaattct tcttggtcat
     9121 aagcgattac tgccttatta agcacaaatt catctgtaat atcgcttata tccgctggtt
     9181 ggaattgcag cagcttattc aacacattgt acaaggattg cttccttggg gcactaggca
     9241 gctgtaaaac cacattgtca gacaaaacat ctagtggcag atccaattgc tgagccagtt
     9301 tgtataagcg ttggatatct gatacttcca tcactttctt ttccatgatt tcaccagtat
     9361 catttttttg taccaaaccg ccgttgaaag tgatgctgta gtcaccttca tctaccagtc
     9421 ctaattcttg cagataatgc gccatcgcag ctaaggggcg tcctgtacat agaacgatct
     9481 tgaccccttg ctgttttgct tgtgctaatg cttgtttatt ctcatctgaa atcagctttt
     9541 gttcatttaa taatgtaccg tccaaatcga ttgctactaa tttgatcatt ttcgttccta
     9601 cactttcgtt tcgttgattt tcgcctcttc attgatatct tccatccgta cagaaacgat
     9661 ttttgataca ccggattcct gcatcgtcac tccgtacagt acatctgctg cgaccatcgt
     9721 tcctttgcgg tgtgtgacta cgataaattg tgtgtcatct tggaaatcgc tcaagtaacg
     9781 accaaaacgt ttcacgttgg cttcatctaa cgctgcttct acctcatcta agatacaaaa
     9841 cggaacagga cagacacgga taatggaaaa gagcaacgcg atggctgtaa gtgctcgttc
     9901 tcctccagaa agcaaactta gactttgcag ttttttgcca ggaggttgta cttcaatctc
     9961 aatacctgtt ttcagcaaat cagttggttc cgtcagtact aattcagctt taccgccgcc
    10021 aaacatattt ggaaagacaa ttttgaattc ttgacggata gcttcaaata cttctttgaa
    10081 tcttgtccgc acttcatcat ccatctcatc catcgtttca aacaattggt ttttcgctgc
    10141 taacagatca tctcgctgtg ttgctaagaa tgtatgtctt tcactgactt gttcgtattg
    10201 ttcgattgaa tttaagttta ctggtcccag tttttcaatc tgttctttta aggatgaaac
    10261 ctttgttcta ctgtcttcga tatcggttgt ttcttgatag tcttgggaag ctttctcaaa
    10321 tgtcagctga tattctgact gcagataggt caaatgatta tctaaaatca tttcagctcg
    10381 gtctttttgg acttctaatt tggattgttc cgacagtcgt gctttttgct cacgattacg
    10441 ctctgccaag actgcttcaa gcttatcgat ctccttttgc cttctatctc gctgctcttt
    10501 ggcttttgcc aactcggctt ttaatgtttc tcgctgagca gataattcgt taatttgttt
    10561 ttccaaactt tcttctgtga cttcatgatc agaaaagtct gctgtcaatg tcgccaactg
    10621 cttttctatc gcttcttgac gttcggttgc ctctgctttt tggactcggg caccgcggag
    10681 ctggatctgc agatgattaa actgctcttt caagacagca agatctgctt gttcttgtgc
    10741 tttttgactt tgtacttgtg ctcttctggc ttccatctgg tcgctttctt gacttaacga
    10801 tttgatttct tcgtcgattt tttgtcgttg cgattcaaga tctgtttgtt tgtctttcaa
    10861 ttcgttcgtt tgcttttcgt aatcttcgat aaactgctgc agttcacgtg tctcaaaagt
    10921 tgagatttgc ttttcttttt caaaacgttc aagttcattt atgatgtttt gtagctgatt
    10981 tgtcgcttct tgttcttcaa aacgtaattg ttcgcctcgt gtacgcaaaa cttcttgttc
    11041 ttcggctaaa cgagctgttt cttgttgcag ctcctgaact tttttttctt gtgcttggag
    11101 ttgtttatct gcttcttcaa attcactagt caactgtttc aattcttgat tttgtacaaa
    11161 caaactgcct tggtttcctc gtttattcgc tccacctgtc atggatcctc cggcattcat
    11221 cacatcacct tctaaggaaa ccactcgata ttgatagcgg atcgtttgcg cgattgcatt
    11281 ggcactagtt aagtctttgg ctaaaaggat cgttcctagc aggttttgaa ccacagtttg
    11341 gatttgatcg ggataactga cttgttcact ggcaatccct ataaaccctt ctacagctgc
    11401 tgcttgtgtc aatatatgag ccggtaattg tcttggtttg attgtcgtca gtggaaggaa
    11461 tgttgctctt cctcctcttt gctgtttcaa atacgtgatg gcttgccgag catctttttc
    11521 attttcaacg atgacatgct gggctgcccc acctaaagct gcttcgatag ctaacgtgaa
    11581 atctgcagga acatcgatca gttcagccac tgctccgaca atacccgaaa gctgctgttt
    11641 atgctgcaag accaatcgaa ccccttggta aaaaccaaaa tagttttctt ggatttcttg
    11701 aaggcttttc ttacgagcac gtagctgctg gacttgattc attaattgat acattttagg
    11761 ctgttctttt gctaatgctt cttggaactt tttcccgttt tgttggatca tttctaactt
    11821 ctcttttgtt tcagttaaag atgattttag ctgcgcctgc ttttgtgtca attcttcttt
    11881 ttgtaagatc aatcggtcta ctgatgcttc tacttccgat tgcttggcta acgtctgctt
    11941 gctttttgct gtttcttgta tatattgacg ttctagatac ttcagttcgt ttcctacagc
    12001 tgcttgttct tgcatcaaat caacatattg atctcttaat tctgccaata attctttcgt
    12061 tgatttttgg tatttttcta attcttgctg tgtttttatg agatttgctt cagctttttg
    12121 gatctctgtt tctttttctg ctgctgcttt catcaagctt tcttgaagct tttcaaaatg
    12181 tttaaccttt ttttgtgctt ctgctaatga agtttgatat tcttgcgagc ttttttgtgt
    12241 atgctttgtc cgctcctgca aaacatcttt ttgtccttct gtctgcttca gtttttccga
    12301 aagatctaat aatacttgct gatttttttc gatcagacga tcggcttgcg cattttcttt
    12361 acgctgcttc gccaaaatgg attcttgttc ttggatcgac tcagatagtt tgcctaattc
    12421 tagattgaat ttcgcaagct gtgcttgttt gttgtcccaa tctttttttg ctgtcttgat
    12481 ttcagcaacc atcaaagaga catcggtttg agtcagtgtt tcttttagcc gtaaaaactc
    12541 ctttgctgcc tcactctggg cggccaaagg agttaactgt tcttccaatt cgtggatgat
    12601 gtcttggacg cggctgaggt tgtcttctgt ttcaaaaagc ttctgttctg ctttcttttt
    12661 ccgctgcttg tatttcaaga caccagctgc ttcttcaaag atcccacgac ggtcttccgg
    12721 tttactactg aaaatcgctt ctacttttcc ttgagaaatg attgagaacg attcttttcc
    12781 taatccagaa tctaagaaca attcttggat atcttttaat ctgcagcttt gtttattgat
    12841 gaaaaactcg ctttccccag tccgtcgata acggcgggtt acactgatct cattgtactc
    12901 caatggcaga taatgatcac tattgtctaa aatcactgtg acttctgcaa tattcagctg
    12961 cttacgggta tcggaaccgg caaagatgat atccggcatt tttccgcctc gcagactttt
    13021 agcagattgt tctcctaata cccagcggat agcctctgta atattgcttt ttccgctgcc
    13081 atttggtcca accactgctg tgacgctatt ttcaaaatca atgactgtct tgtctgcaaa
    13141 tgatttaaaa ccggctattt caattcgttt caaatacaaa gctttccctc acttactttt
    13201 acagtgctgc caatgcattt tccgccgcag cttgttctgc taattttttc gatttccctt
    13261 gtccagtgcc aatcaattga tcatctacat aaacttcgat ccaaaatact cgttcatgag
    13321 ctggaccttc ttcattgatc aatcggtatt cgattgatac atcacctgat ttttgcaaga
    13381 cttcttgtag ttttgtttta tgatccatct catgtgaaaa agcacctgct ttgactttag
    13441 gaaaaatcac tttttcgatg aatgcatggg tcgtgtcgaa tccctggtca agatataacg
    13501 cgcctaaaaa agcttcgaat aggtcgcaaa gcaatgctgg tcgcgtacgt cctcctgaat
    13561 tctcttctcc cttgcccaac aaaacatatt gatcaaagtg acattctttg gcaaatttcg
    13621 acaagctgtc ttcgcgaacg atcgcagctc gtgtttttgt caattttcct tctggcaaat
    13681 gaggatataa acggaataaa tactgagaaa ccatcagttc taaaacggca tctcctaaaa
    13741 attcaagtcg ctcattatcc gataattgca gattgcgatg ctcattcaca taggatgaat
    13801 gggtaaaagc ttgttcaagc aaatttagat cgtgaaaaac gatgttatag ttttctttta
    13861 gttcttttgt cagctggttg tccattttcc acattccttt tatggatatt ttttcgatac
    13921 ttccttatta tactggtttt ttgtcaaatt tgaaatggtt cggtcaaaag gtaagtaaag
    13981 ctttaataat cctagtgatt gacgagaagt tgtgaaaaat tcgtttagtt tcgagtatga
    14041 agaaaaaatt ttgtgaaaat ggctttccat atttttgcat aaatttgact taataccgag
    14101 aaactggact ttgaacaccg tttattggag aagtcatgaa atcagcgcct tgacctgaat
    14161 attagagaaa taaacagaaa aatatcaaaa aaccgaaaaa ggacatcgcc ctttttcggt
    14221 catttttagt gattatgaac gtgaatctgc tgtaacagaa acatttgccc atgggtttgg
    14281 tacatcataa gaccaatccc agtctttgac acggtcgttg actggcaata cttcattacg
    14341 gaacaacgtt ggaatcgcaa atgcttcttc aaatgcatat tgttgccatt tttgatatgc
    14401 ttctttttgg tactcttcat caaatgcttt gcttgaactg atatcgttga tcaattttgt
    14461 attttcttct gattcataac gagtgtagtt gaacgcagca tttggtcccc atagtccact
    14521 tggatttgga tcagttcctg tattccaagc agcttggtaa acatcaattt caggatcatc
    14581 gttttctaat ttatcataga aagaattgaa ttcgatcaga cgacctgttg tgtattggac
    14641 atccaaacca atttctttcc attgttggat atagtaatct gccaatggtt gcgctgtttc
    14701 cccaccggac atcgaagcaa agttgattgt tagtttattt ccatctttgt cttctcttaa
    14761 accatcgtca tccacatctt tgtagccagc atcatctaat agttttttcg ctttgtctag
    14821 atcttgtgtg aatcctttca tctcatcatc atggtatgac tggaatactg gaggaatcaa
    14881 tgaagtcgca ttgcttctca aacctgcata gaaacgttca cctactgcat tattatctaa
    14941 tgcatagccc attgcttgac gtaatgattt atcagccatt tttgaatctg gattgtattc
    15001 cacactcttt gtatctgcat tccatttacc taacttgaat cctagataag tataagaagt
    15061 ttgttcacgg cctaagtttg tatagccagc aatttcttta taggtatcgt aagtatctgt
    15121 tggcatttgg aaaatcatat catataattt agagttcaat gcttcagttg cacttgccat
    15181 cggtacagat ttcatcgtga tctttttcaa tttaggcgta ccattccagt agtgctcgtt
    15241 tggcaagtat tctactgatt ctcctgcaac gatattactc atgtaataag ggccaaaagt
    15301 cactggattt ttacgtactt gatcgcttga ttccatatct ttgattgcta caccttctaa
    15361 cgtatgtttt ggtgctgcat atgaccagat accaccaccc gattgaagca tgcttgggtt
    15421 tacttcttta tattcgattt caactgtttt gtcgtctatt tttttgattc cggagattgt
    15481 gtcggcactt ccatcatgat attcttccat acctacgatg ttacggaaag tgtcgtcata
    15541 acggatacca gtgtaatctt tattcccaat gatttcgtaa gggaaaatca catcgtccgc
    15601 cgttacatct tcaccatctg accatttgac gttgtctttt actttgattg tcgctttttt
    15661 cgtatcttca tctagatcaa gcgttgcagc tccgtcgtct gtgattctga aatcgtcatc
    15721 gtttgcaaat agcggttcat gagaaggagc cataaaagct gcatcatatg aatcttggta
    15781 aaactcccac tggaacaatc ctttaaactg actgtccgtt gcgaccgcag cttctaattc
    15841 gccgtcttcg attgcatcat cattattttt tgttgcaatt ggaaaagtta attcctcttt
    15901 gttattgccg ttgcctccag agttgtttcc agaggtatct gtacctccgc ccccgcatgc
    15961 tgcaagaacg acagcagaca tcagcgctgc aaatccaaaa atcgttttct tcattcctat
    16021 ttcccccttc gcttctatac tagcctaacc gttggcgcgc atctgcagaa cgcttgaatg
    16081 cttgtccaac ataattgata ctcaacatca aaactaaaat aaggattgat gcgggtaacc
    16141 agatccattg ccgattcact aagacttctc cgttacttgc ataaccgatc aatgtaccta
    16201 aactaggtac atttgccggc aagccgaaac ctaagaaagt caaagttgtc tcgataccga
    16261 tgtttgccgc aaagttcatc gtcaaattag taatgattaa tgaacttaga ttcggcatga
    16321 tttcgcggaa catgatttta aaatcattgg ttcccattgt ttttgaggca ctgacataat
    16381 ctcttcttgc ttcagataat gtcttgcttc ggaataatcg ggctttagct acccaataaa
    16441 atgcactcat gatgaatata aaggaccaga cattgtatct ggaaataatc gatacgaaga
    16501 cgataatgat cagcatgatc ggtagaatca tgataaaatc aacgatccgc atcaagatgt
    16561 tgtcaatgat ccctccgtag tagccagaga tgatccctaa tccaaccccg atgatcgaag
    16621 taaggatcgt gatcgcaaat ccgatcgtaa cagagtttct agcacctatg atcaattgtc
    16681 ctaatacatc acgtccgcct tcatctgctc ccagaatgaa cttttgtcct tgtgtcgaaa
    16741 ctgttcctgg tgcagcgtat ttgtcgaaga tactaacata catcactttg tcttgatcgg
    16801 tcagcatcgc accgataaag atcactaata ataaaaccac tagcaaacct aatgaaaaaa
    16861 tagcaatttt atctttttta aattctcgag caatcattcg tatccccata ggaggaatac
    16921 tttcttgaac tgctgtattg tttttttcac tcatcgatcc attctccttt ctgattggtt
    16981 ttattgaatc cgaatacgcg ggtcaacgat actcatgatg atatctgaaa gtaatgtccc
    17041 cactagcgtc gcgattccta aaatcaatac taaggatgtg atcacggtat agtcccgttg
    17101 gccgatggaa tcaataaata gttttccgac tcccggataa ccaaaaatct tttcgataac
    17161 tacagaacca gcaatcaacg aagtgatttc ataccccatt tgagaagcca tcggcaacgc
    17221 tgcatttcgg aaaatatgtc gactgaatac tttattcgtc ggtacccctt tggaacgggc
    17281 tgttctgaca aaatcgagcg atttcgaatc gattacttca cttcgcagat attggattgt
    17341 gacggcagtc gccaaaaatg cttgggtaat tgatggtaac agaatatatt tcaaacggtc
    17401 tatccattga gtgataaatc caccttgcaa acctagtgtt tgtgatccac ttgttgggaa
    17461 ccagcctaaa cgataaccaa aaacaaatag catgatcaag ccgaagataa ataaaggaac
    17521 agcaaaacta aagaagttgt agattacgac aatcttatcc aagattgaat tttgataccg
    17581 gccagcaagc atccccagag gcacagcaat caaataagta aggacaactg tcaagatcga
    17641 tagatataaa gtgttgatga tccgttcgcc gatcaagtca gcaacagagc gtttgtataa
    17701 gaaactttga ccaaaatcgc cttgcaaagc atttccgatc caacggaagt actggacata
    17761 ccatgggtca ttcaagccag ctgcttgtcg catctgttcg atcactgccg gatcctgatt
    17821 tggattgatc aaaccggtaa atggatcccc cggcatcgct tgggctaata agaagatcag
    17881 aacacttaaa ataataactt gcgggatcat caataaaaca cgtcgcagaa tcgttttcca
    17941 cattagcttg cacctccatc tttcaacgcc actttgtgtg tagttgtgat cgtacgcaaa
    18001 tcgtagactc gaccagtcgc atcatagtat tctttttggt tttggatata ctcttgttct
    18061 actcttcgtc gattttcttt gtggctttcc ctattttcta catcgatttg cggaatagct
    18121 gaaagcagac gtttcgtata gatatgtcgc gggtcattat aaatgtcttc tcttgtaccg
    18181 atttctacaa atcgaccttt gtacataatt gcgatattgt cgcacatgtg ttttactaca
    18241 cctaaatcat gtgaaatgaa taaatagctg aggccgtatt cttgctggat tcgtttcatg
    18301 aaattcaata cttgcgcttg aacagataga tccagagcag atacaggttc atcggctacg
    18361 atcagttttg gattggtagc aacagcacgg gctactccta gacgttgacg ttgtccgcct
    18421 gaaaattcat gaggatattt atatagcgca tcttccggca tcccaacgat atctagcagt
    18481 ccttttacct ttttcttttc ttcttgatcg cttaaccgtt caaaatttcg gatgggttca
    18541 gcaatgatat ccaacactcg ctttttagga ttcaaactgg acattgaatc ttggaagatc
    18601 atctgcacat ccttgttata acccattttt ttgcggttgc ttcgcttcgt tacatctttt
    18661 ccttcataca tgatttgtcc ggctgttacc ttttctaatc ccacaacggc ttttcccgta
    18721 gtagattttc cagagccaga ctctcccact aaaccatatg tctttccttt ttcgataata
    18781 aaatccacac catctacagc atatacatga tccgttactc gattgaagaa tccactgcga
    18841 atagggtaat gaactttcag gtctttgatt gtgattaatt ctgtcatcta cacctctcct
    18901 ttttcgtctc taaagtggaa atgcttatag caggagcaac gtaccaagtg gttcggtgcg
    18961 acttcatgaa ggataggctc ttcttcatga tcgcttgctg gtatccatgg aatacgtggt
    19021 gcaaaccgac aacctttacg aggcatgttt ttcagagaag gaacgacacc ttcgatcaca
    19081 tggagttctg catcatgatt tccttcttgt ggtatagagt tcagtaaaga gcgtgtataa
    19141 ggatgcttag gatgatcaaa cagttctttt acacctgcta cttcaacgaa ctgtccgcca
    19201 tacatcactg ccactcgatc agccatttct gctacgactc ctaagtcatg agtgatcaag
    19261 atgatccctg tttttgtttc ttcctgaagg tctttcagta aatcaagaat ctgtgcttgg
    19321 atggttacat ctagggctgt cgtcggttca tctgcaatga cgattggagg tttgcaggcg
    19381 attgcgattg cgatgatgac acgttgtctc ataccgcctg atagttcatg gggatattgg
    19441 cgtccaactc tcgcaggatt aggaatccct acttgttcta gcaattctag tgcacgagct
    19501 tgacgctgtt ctgcattcat ctttgtgtga taggaaagtc cttctttgat ctggtcttcg
    19561 attctcatga gaggatttaa tgcagacaac ggatcttgga aaatcattcc gatatcattt
    19621 ccgcggattt tattatagag cgtttcgttc aggtcgatta aattcagatc gttatataaa
    19681 atctcacctg taatttttgt gttattagga tcaagtaatc ccataatcgt agtagccaag
    19741 gtacttttcc cgcacccaga ttccccaacg attgctaaaa tctcgttttt gtctaaagta
    19801 aatgaaacgt catctactgc atcgtaatag tcatctttga gtcggaatcc tgtatgtagg
    19861 ttctgaactt ctaataattg gttatcggac aatcgaatac ccctctctgc ttcctaaatg
    19921 gaagtttttc ttaataaaaa aggacattac acagaataaa tcgttttata acaaaggtta
    19981 ttcttatctt tgtgtaatga attattaata attatataga aaaccgcttt actttgcaac
    20041 ataatttctg tttatcgcca gaattatcag aaaaatgact tttacgtgtc aaaaagatta
    20101 aaaaaagaag gataaaaagc tgcatgcagc tttttatcct tctttgcttt tatttgaaat
    20161 tatttaagat ggctgctgat ataatctact gcgccaccga ctgtttcaat ttgttcagcg
    20221 tcttcatctg aaatttccgt accgaattca tcttcaagct ctaaaacgaa ttccataata
    20281 ctaattgaat cagcttttaa atcatctttg atgttcaatt catccgtcac tttatcggta
    20341 tctatctcaa aatgatttga aataattttc gctactttgt taaatacttc ttcgcgagtc
    20401 aactttttca cctccaagaa tcatacactt gtcaattacc tacaccatgg ctaattttac
    20461 tggcattttt caggaatgtc cacctttttt tcaatggaac ggatcgcttc agatatccaa
    20521 atactattct tcgctttttt caaattgttc gactaattgt ccaactacat ctgtctcaag
    20581 cattgtgtgg atttggcgga tcgtatagcg aacagcatcg gggcctgttg ctccatgagt
    20641 tttaatgact ggtgctttca agccaaacaa aacggcaccg ccatgtttgg aataatccat
    20701 ttctgctttc aaagaacgta agccgtcttt taaaagcata gcgcccattt ttcctttgat
    20761 cccttcattt agaatagcag attttaatag attcatcata ttcattgctg ttccttcaat
    20821 agatttcaaa acagcgttgc ctgtaaatcc atctgtgacc actacatcag ccacaccatt
    20881 gagtagctca cgagcttcta cgtttccaat aaaattcaag cttgtttcat tctgaaggag
    20941 ttcataggct tttttcgtca cttcactacc ttttgttgct tctgttccgt tgtttaagag
    21001 tgcgacacga ggacgtccga tacctcggac tttacgtgca taaaaagaac ctaaaatacc
    21061 gtattgcaac aaatgttctg gtttgttttc tgcgttagcg ccaagatcca gcatatcgaa
    21121 accggcacct tctttcccta ctactggcaa ggtagacatc aagccagggc gttcgatatt
    21181 tttgatacgt cctacgatga acaaccctgc tgctagtagc gcgcctgtat tccctgctga
    21241 aaatacagca tctgcttcac cgtttttcac tgcttgtgca gcaaggacca ttgatgcagt
    21301 cttttttcgt cgaattgctt ttactggctc atcatcacta ttgatttttt cgtctgtatg
    21361 gataatcgtg atgttttttt catttgttaa gtatttcttg atttctgctt cttttccgta
    21421 aagtaagaat tctacttctg gaaaatcttg ctgcgctaaa gccacgcctt ctacaattgc
    21481 ttgaggtgcg tgatctccgc ccattgcatc tacggcaatt ttcattgttt ccactccttt
    21541 ttccgtcatc agtataaagt atagcacagt ctgctaatcg aaataacgat tatcctgatt
    21601 ttctatttgg aggctttgcg ctaaaggctg atactcagga agcatctgcc aatcctttat
    21661 ttgccaaagg gctgcagcct cttctctggc tacctccaaa acatttccat ccgtcaccaa
    21721 gtcagcaatc gcaaattgag gcagaccaga ttgacggaag ccaaatactt caccaggacc
    21781 acgtaattcc agatcttttt cgcttagtac aaaaccattg gtggtctctg tcatgatttt
    21841 catccgttcc acacctagtt catttttagg attagcaact aagatacagt aggaagcatc
    21901 acttccgcgt ccaacacgtc cacgaagttg gtgcagctgt gccaatccaa atcgatccgc
    21961 atccatgatc agcatgacgg tcgcgttcgg cacgtttacc cctacttcaa ttacagtagt
    22021 cgaaactaat atctgcatct ggttgtcttt gaaggcttcc atgattactt ctttttcctg
    22081 attcttcatt ttaccatgta acaagccaac ttcatatttt ggtgcaaata gttcacgaag
    22141 tttttcatag atttccactg cattttttac atctaatgct tctgattctt caatcagcgg
    22201 acagatgaca tacatttgat gtcctcttgc cagttctttt ttcgtccaat ccagtacact
    22261 gtccaattga ggtgttctta cccagcgtgt ttcgatcggg atacgtccag ctggaagttc
    22321 atcaatgata gatacatcca tctcaccata tgctgtaata gctaatgttc taggaatcgg
    22381 agtcgctgtc ataaataaga catctggtcg ccagcctttt tcacgaagta ctttccgctg
    22441 gttcacgcca aaacggtgct gctcatccgt aataactaga cctaaattct gaaattcgac
    22501 tccatcttgg atcaatgcat gtgtgccgat cacgacgtta atttcccctt gctctagttt
    22561 ttcaaggagc tcacgtcgtt cttttgcttt cgttgacccc gtaatcaatg cgatggttac
    22621 ttcattggga tcaaacagct gttgcaagct ttccatatgt tgttgtgcca agatttctgt
    22681 aggcaccatc aacgcacctt ggaaacctgc tgtaacggta gcgtataacg caatcgcggc
    22741 aaccactgtt tttccgctac ctacatctcc ctgcaaaagt cgctgcatat gctgcggact
    22801 gcgaagatcc cgacagattt cattcgttac acgtttttgt gcatttgtca gttcaaaagg
    22861 tagcttttga gtaaattgtt tcaatttttg gacatcataa aggatctcca aaccattcgt
    22921 ttctgctttt tctgcttttt tcaatccttg aagacgcatt tgaaataaaa agaactcttc
    22981 aaaaacgaca cgtcgtttgg cttgatggtt ttcttcgtgg tcttttggaa aatgcatact
    23041 tcttacagca gaagctcggt caagcagtcg atatttctcc acaaggttgt ttggcagatt
    23101 ttcttcaatc aatgtgccgt attcttgaaa cccttttttg attagatcaa ctaatgtcgt
    23161 ttgacgaatt gatttgttga cgtgatatat cggcgaaaag tcatccattg tttgagaacc
    23221 taaaatcttc atcccgttca aggctttacg ctttgcatcc catttaccgt atacggcgat
    23281 ttcctccgat aaaatgactt tttcttttag ataaggttga ttaaaaaaag aaacgttgaa
    23341 cacatcatgt tcttgcatca tacgaaattg caatcggctt tttttataac caaatcgatt
    23401 catcacagga ggcgagacta cgattccttt caaagtaacc ttttcttgat cctggatctc
    23461 gtttagattt ctttcctgaa tatcttcgta gcgaaaagga taataagtca gtaagtcttc
    23521 aatcgtttgg atgcccaagc tggcaagact agctgctctt ttttctccga ctcctgccag
    23581 catagtgatt ggatcatcta aagaacgcat aaatttttcc tccctccttt aacaaaaaaa
    23641 ggacttggtc agcgatcata cctgatcact aaccgaaggc cttttatgaa tgaatactgt
    23701 gttagaaaga tattctgaca cagaaattat tcagcagaaa atagataagg atagacaggc
    23761 tgatctcctt ggtggatctc gatttccaaa tcaggatgtt tttctgttag tttctcagca
    23821 aattcatttg cttgttcaac tgttccttca tcacctgcca agatcgttac gatctctgtg
    23881 tcctcattga tcatttgttc tagtgtactt aacgctgttg ctgtgatatc tgggttagag
    23941 atcaggattt ttccatcgat catgcccaaa aagtctcctt ctttgatctc tacaccgtca
    24001 attgaggtat ctctgatcgc gtgagtgact tgaccgctca caacgctaga caacatttct
    24061 gtcatcgttt ctttgttttc ttctaaagaa gcttgatcat tgaatgaaag catagctgtc
    24121 aatccttggg aaatcgttct tgaaggaaca actgctactg ggatatctgc tacttctgct
    24181 gcttgatcag ctgccatgaa gatgttctta ttgtttggca agacaatcac tttatctgcg
    24241 tttacttctt gaatcgcttt aacaatgtct tccgtacttg gattcattgt ttgtccacca
    24301 ctgataacat aattagcgcc taaactttca aacagctctt ggacgccttt accagcagcg
    24361 atcgctacga taccatacgg gatacgtggt atttcttttt tcgcaaactc gttcacttct
    24421 tcatcgtgtt ccaaaatcgt ctcatgctgc aagcgcatgt tgtcgacttt gattttgact
    24481 aatgaaccga atttttgtcc ataattcaag acttctcctg ggttttctgt atgcacgtgg
    24541 actttgatga tctcgtcatc attgaccaca agtaaagaat ctcctatacc atctaaatag
    24601 ttacggaatt cttcgtaatc gaatgtacta tcgactgtag gtccttctcc gatttttacc
    24661 atgatttctg tacagtaacc aaaatggata tcttctgtcg ccatttgacc ttggacactg
    24721 cgatgatgtt ctgcattgac catatcatcc atttctgctg gagaaggttc atatgtttca
    24781 tccgcttgga attctccatt caatgcactt aaaaagccct cataaacaaa cagcagtcct
    24841 tgccctccac tgtctacgac accgacttct tttaggactg gaagcaagtc tggagtttta
    24901 gctaaagcac gctttgctcc ttttacaaca gctgtcatta cttcaacgac atcatcagta
    24961 ctttttgctt tcttttctcc agctttcgct gcttctctag ctactgtcaa aatcgttcct
    25021 tctactggtt tcattactgc tttatacgca gtttcaactc catgagtgaa ggctgcagca
    25081 agatcagaag catttaatga gacaacatct ggaatttgct tagaaaatcc acgaaacaat
    25141 tgagacaaaa taacgccgga atttccgcgc gcacccatca gcaatccttt tgaaagcatt
    25201 gctgctaatt ctcctacttt ttctgaggtt gaatctacta ctgccttagc tccactagtc
    25261 atagatagat tcatatttgt tcccgtatcg ccatctggca cagggaatac atttaatgaa
    25321 ttgacgtatt ctgcatttac ttgcagacga ttcgcacctg cctggaccat ttcctggaac
    25381 tggcttgcac ttatttctgt tacactcacc tagataatcc tccttcagat cgactgctga
    25441 gttaccagcc agtctcctgc ctagtctggc agtacacgaa caccttgtac aaagacatta
    25501 actgaattgg cagttacgcc taataatgcc tctagattgt atttcacttt ttcttggacg
    25561 ttgcgggaaa cttcagagat tttcgtacca tagctgataa tcgtataaac atctacagca
    25621 ataccattat cttcttggcg gacaactact cctcttgagt aattttcttt acgcaaaatc
    25681 tcagtaatat tatctttaat ttgatttttg ctggccattc ctacaacacc gaacacatct
    25741 gtcgcggcgc caccaacaac tgttgcaatt acatcattgg taatttcaat ggttccagct
    25801 gacgtttgaa tttttacagc catgaatgag cctccttaac gggtaaaaaa ctcccatttt
    25861 tcttctatca tattttatca tagcgcaact tgaaataaaa ggcagttacc caaagtactc
    25921 aaaaaccttg caaaatttca ggtataacct tgtttaattt acaattattc ttctcaattg
    25981 tcaattgtat ctatgaaaat gtacttgcaa attcccattc tatatgatat gttagtctgg
    26041 tatgagctga attattctat tgctccaaat caaggcaaag gaggaaatta ccatggcaaa
    26101 agtttgttac tttacaggtc gcaagacaaa aagtggaaac aaccgttcac acgctatgaa
    26161 ctcaacaaaa cgtactgtta aacctaactt acaaaaagtt cgcgttatgg tcgacggaaa
    26221 acctaaaaaa gtttgggtgt caactcgtgc tttgaaatca ggaaaagttg aacgcgttta
    26281 attgaatgat acttggaccg ttcagatagt gaacggtttt ttatttaata aaaattttta
    26341 agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata cgaacaaatt
    26401 atgaagttat ttattctaaa tttta
//
LOCUS       DAFGFZ010000030        26383 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000030 DAFGFZ010000000
VERSION     DAFGFZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.030"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(186..1130)
                     /locus_tag="LR726_001086"
     CDS             complement(186..1130)
                     /locus_tag="LR726_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6216192.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1351..2565
                     /locus_tag="LR726_001087"
     CDS             1351..2565
                     /locus_tag="LR726_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6216193.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2587..4602
                     /locus_tag="LR726_001088"
     CDS             2587..4602
                     /locus_tag="LR726_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL6216194.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4685..6058)
                     /locus_tag="LR726_001089"
     CDS             complement(4685..6058)
                     /locus_tag="LR726_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL6216195.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6084..6368)
                     /locus_tag="LR726_001090"
     CDS             complement(6084..6368)
                     /locus_tag="LR726_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6216196.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6370..8385)
                     /locus_tag="LR726_001091"
     CDS             complement(6370..8385)
                     /locus_tag="LR726_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL6216197.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8564..8869)
                     /locus_tag="LR726_001092"
     CDS             complement(8564..8869)
                     /locus_tag="LR726_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL6216198.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8958..9989)
                     /locus_tag="LR726_001093"
     CDS             complement(8958..9989)
                     /locus_tag="LR726_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL6216199.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10196..11071
                     /locus_tag="LR726_001094"
     CDS             10196..11071
                     /locus_tag="LR726_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL6216200.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11223..12065)
                     /locus_tag="LR726_001095"
     CDS             complement(11223..12065)
                     /locus_tag="LR726_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL6216201.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12157..14034)
                     /locus_tag="LR726_001096"
     CDS             complement(12157..14034)
                     /locus_tag="LR726_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL6216202.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(14031..15452)
                     /locus_tag="LR726_001097"
     CDS             complement(14031..15452)
                     /locus_tag="LR726_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL6216203.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15455..18844)
                     /locus_tag="LR726_001098"
     CDS             complement(15455..18844)
                     /locus_tag="LR726_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL6216204.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19101..20492
                     /locus_tag="LR726_001099"
     CDS             19101..20492
                     /locus_tag="LR726_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6216205.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20565..21212)
                     /locus_tag="LR726_001100"
     CDS             complement(20565..21212)
                     /locus_tag="LR726_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL6216206.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21220..21906)
                     /locus_tag="LR726_001101"
     CDS             complement(21220..21906)
                     /locus_tag="LR726_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216207.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21912..22985)
                     /locus_tag="LR726_001102"
     CDS             complement(21912..22985)
                     /locus_tag="LR726_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216208.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23207..23881
                     /locus_tag="LR726_001103"
     CDS             23207..23881
                     /locus_tag="LR726_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6216209.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23886..25226
                     /locus_tag="LR726_001104"
     CDS             23886..25226
                     /locus_tag="LR726_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL6216210.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25375..26361)
                     /locus_tag="LR726_001105"
     CDS             complement(25375..26361)
                     /locus_tag="LR726_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL6216211.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7686 a         4981 c         4833 g         8883 t
ORIGIN      
        1 ggaaacgaat tcctgaatac gcataacaga taaatgacaa atgtttacac ctatatcccc
       61 tgataataca accaaaagtt tagaactgaa aataaatcca aaaaaattcg ctgcaacgat
      121 ataataagac agcaatcgca cctaaaacaa ttttccttca tatgctttag tgaaagattt
      181 tcttcttaat gtttatttct ccattcattg ggagtacatg ttgaatactt cttgaataat
      241 ttgaataaag aagtcgtttg tttatatccg atacgattag cgatttcttc caacgaaaaa
      301 ctcgtttctt gaatcagttc caacgcaaag tcgaattttt tctttagaac cagttcttga
      361 aaggaagcat caaaatacat tttcaattta ttactaagat agaccggatt gtagccaaaa
      421 tgagctgctg tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact
      481 ctaagcatct cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa
      541 tgagtcaatt cgatcagtaa tagtgatgta tagagattta acgaacggat cttatgactg
      601 tatgtatcaa aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt
      661 ccgccgcgaa aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc atttatcaaa
      721 aaagaagaaa gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt
      781 tcttctttta ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga
      841 gtagcttctt tatcaatgat caccacgtca ctttcttcca tctctatcct ttctccatta
      901 ataatttgta aacatgtacc gctatacata taattgattt caacaaaatc atgtgtatgc
      961 tcgggaactt ccgcatatcg acggtgttta ctgatatgaa tatttccctg ttgaaataga
     1021 tcccctcggt ggatttttgg cggtgtctga agatggtaag ctaagtttaa atcattgata
     1081 ttcattcctt gttcacgctg cagagtttcg attgattctt cgttacgcaa aaaaaatgtc
     1141 taattcagct agttccaatt gactccctcc tttttgtaag cggtttcttt cgcttattag
     1201 ttcacgctgt actgctatta tatgcaaagc tatttttggt ttcataaaga ctttctatgg
     1261 ttgggacatg aacttcctgt gattccgcta ttgagagaat aaccacttca tactatattt
     1321 ttgataagcg cttacaaaaa tgaaaggaac atgcttatga ataaaaagat gatgaaattt
     1381 tctctgctgt cagtctctct tattttgact tctgctggtg cagtcacgtc tgcaattccc
     1441 agtatgtcag ctacttatgc cacgcgttct ttatctgctg ttgaattatt aacgactgct
     1501 ccctctttta tggtcactat ttttgttttg attagtagtt ttattgctag gaaaatcgga
     1561 caaaaacaaa ctgtgatcat cggattagtg attgcatcga tttgtggtat cctccccctc
     1621 ttctcacaaa gttacagtat tgtcttagct tcaagagctg gactaggtat tggctttgga
     1681 ttgatcaatt ctttggcagt cagcatgatt gctgcctttt atcacggaga tgaacgagcc
     1741 tctatgattg gttttcaaag tgcatttcaa ggatttggtg cagctttaat gacatttgtt
     1801 gcaggacagc tagtacagtt cggttggcaa tatacctttt tagtctacgc catcagctta
     1861 cctattcttg tcttgtttgt tttgtttgtt cctacacctg aaacaactga acttcaatcg
     1921 catggcacag ccccaacaag acaagtaatc aaaacgaatc ctaaaatgat tagttatggt
     1981 atttttctct tgctggtcat cgtggtatat aatgctgtat cgattaaatt agccacagtc
     2041 ctgacgactt attctctagg aaacgaaact aatgcagcga atatcttaac aatcatgcaa
     2101 ttggcaagta tggctgccgg agtttgtttt ggctggcttc agacaaaagc acatcgctat
     2161 atgcttacac tttctttact actgatggga atcggctttg tattgattgc cactgtgata
     2221 aacctgtatg tgattgggtt tggtgcgatc ctaacaggaa tttctttttc gttattcatt
     2281 ccttacttat tcaaccaagt aagtatccaa gcgcccaagt acgcggagag tttcaatact
     2341 tcaatcttat tggttggcgc taatttaggt actttcattg caccatatgg tttgctcttt
     2401 ctctcttttg cttctcttgg gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg
     2461 ctgttgatta gcgcgcttat tagccttcta gtagtacaca ggacgaatag aaagacagtg
     2521 ataagagtag atgaagaaat aaaagaacaa ttgacaacaa aataaatttg atggaggtcg
     2581 tgtaaaatga caaatgaaaa acataaagat agatggcttt ggtatcctgg agactttgag
     2641 atccgccatg gactgctaca aaatttccaa cgggaagaac gaggttttga ctggccagcg
     2701 tactggtaca tggatgattg ccatcggaac gtcaagttta agagatatta ctttcttgat
     2761 caaccttcca tgtttaaagt gacgatccaa ggagttgggt atgtagaaat taatggacaa
     2821 aaacatccat gtggaaaatg gctgacttgt ccagctggta aagcaaaaat tcgtatcttt
     2881 gtaggacata catcagggct accagcgatg tttatcgagg gagacgaagt aaaatcagat
     2941 attggctgga cagcaagtaa ttttattgaa gaatatccag ctggctggag tccgctttat
     3001 gtcgatatcg caaaagatcc aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc
     3061 actgaaacgg agattggcgg aggaatctta ttcgattttg ggcgagcagt gaatggctgt
     3121 gtagaactaa tatctctgga tgaccatatc gaatctatca ctctatgtta tggagagtcc
     3181 gatgtagaag ctatggatat cgattattgc tactataagc aaataggaat gacaagaaaa
     3241 atgaaagctc gtaaacgcgc ttttcgctat ttgtttatac cagaggtttc tactggaatg
     3301 gttcaacttc aagtttttca tgaattttta tctctttcga aacaagctgc tttttcaagc
     3361 gattcttctt tactgaatca gatctggtca gtatcagaag aagccttcaa gttatgtagt
     3421 gatttgtttt ttatcgacgg tatcaaacga gatcgctgga tttggtctgg agatgcgtat
     3481 caatcattca tgatcaatca atatcttttt tttgacgagg aaatcaataa acgaacgata
     3541 ttagcattaa gaggacaaaa tgaaatcaag cagcatatga atactatcgt tgactattcg
     3601 atcttgtggt tgattggtat cgaaaaccac tatatgatga cgaaagacta tgaattttta
     3661 cgcatcgtct accctaagat gcaatcatta atgagatact tgatggagca aacgaatgaa
     3721 ttagggttta tttatgggag agcaaaagat tggatattta ttgattggtc tgaaatggat
     3781 aaagaaggaa cgcttgctgc agaacaagtc ttattactga aagcctatca ttccatgatc
     3841 acttgcggag atatcttagc tgaaacatat ggagagaacg aaaattatca tactcatctc
     3901 tatcaagaaa aatatcaact attgaaacaa aacctatttg actacttttg ggatgaagaa
     3961 cttggcgctt ttatcgattg ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat
     4021 attttcgctg ttttattcga ttttgctaac aaagaccaac agcaatcgat cttaaagaat
     4081 gtcttattga atgacactat cacacagatt actactccat atttcaaatt ctatgaacaa
     4141 gatgctttat gcaaactcgg acagacgaat atcgtctacc aaacgattct tgattattgg
     4201 ggagggatgt tatctcatga tgcggtaact ttctgggaag aatacgaccc tactcaaaca
     4261 ggaaatgaaa agtacagtat gtatggagat ccttatggaa aaagcttatg ccatgcatgg
     4321 ggagcaagcc caatctatct tttaggacgc tattttatcg gtttgtatcc aacaaagcca
     4381 ggttatgaag aatttatgat acaacctaat ctagattcat ttgaaacact gaattgtacg
     4441 ctacctatca aagatggaaa gatttcgctt gaaaaaacaa aagataagat cgtgattact
     4501 tctgatagaa caggcggaac agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa
     4561 cagccacttg tcttaacaag ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg
     4621 acatatcaga agactttaaa tcttcttctg atattgtcca agcaattttt attttgaagt
     4681 ttgcttaatt ctgttcttta gaaacaatgt ttgttctttt tccggcaaaa agaaagctag
     4741 ataatacgac aagtaaaaag aaagcgatga ttccaactgt caaggctatt tgaccgtatt
     4801 ctcctagttt cccaagtaca agtcctggta ctagataatc agtatcggca aatgtagaag
     4861 aagcgatttt aagatctcct aatactggaa ggataaataa tggcagccaa ctaatcaata
     4921 agctattgaa agcagaacct aataccgccc ctcttctacc gccagtcgag ttgccgaaga
     4981 caccagaagc agctccgcag aagaaatgtc ctactacccc aggaatgata actgtagttt
     5041 gcaagcctat catagcaaac atggatagtg ttcctacaaa gaagctaaca aaaaagccaa
     5101 ttaatacagc attaggtgca tatggaaaaa cgataggaac atctaatgct ggtttagaat
     5161 cgggtacgag ttttttagca atcccttgga atgctggaac gatttctgcc aacaccattc
     5221 gaaccccttg aaggacgata acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa
     5281 agattacagc gttgtctcca ttgcttagtt ctctttcaac aaatccagga ccagctaaca
     5341 aagcaagtgc gacatatacg atcgacatca agagcatgat cgatacagta gaatctcgca
     5401 aaaagctgaa acttttagga atctttatgt cctctgtaga acggtctttg tttccaaaaa
     5461 actttccgat ccatccacta gaagcataac cgatatttcc ggtatgccct aacgcaactt
     5521 gattgtttcc agtgatgttc ttcataaacg gttgacaaag agcaggcgta atagttaata
     5581 gtgttccttc gaagaccccg cctaataaga tcatccacaa atttgcagta aaccccgcgc
     5641 ttactaaaat gactgctgtt agacaagaaa cataaagcat tgcttgacct gtaagaaaaa
     5701 tgaatttata tcttgtgaat ctagcaagta aaatattcac gatcatccct actagcatga
     5761 tcaaagcagt aggtgtaccg tattgttgca aagcaatcgc cactactgct tcattgctag
     5821 gaactactcc ttgagtatgc agagctgttt ggaacatcga agcaaaaggg gttagtgaat
     5881 tactgattaa accagcaccg ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc
     5941 cttggatcac ttctgaaaat ttcttcttct gaagaacgag accaagcagt gcaatcaaag
     6001 aaacgaggat ggagggctga ctgacgattg acacaaacaa attcaacaat ttatccattg
     6061 atagttcctc ccttgaatca tttttattct tttgacaaga tcttcgttac tttttctttt
     6121 acttcttcgc tgtcaatgat cgattgcaaa ggtacgagct tttcatcagg tatagaagtt
     6181 agcgtatctt tcaaagaatg ctcaatgaag aaataatcag ccatatcgct agatacactt
     6241 ccaacatccg tatgacttac ttcaaactcg tccacatctg ctccaagatc tgcaagtgct
     6301 tgtttgatgt tcatctctac cataaaactt gttcctaatc cactttgaca tactgcaaaa
     6361 attttcatat cattcgtctc cttttcttat gatcgctaat atttcttctt ttgttgatgc
     6421 gtgtaataaa tcttgaagat tttgtttatt agaaagtagc tttgttaaat ttgccaaagc
     6481 ccgaagatgt gcctcattat cgatagcagc taaacagatg aacaaactaa tgggatgggc
     6541 tggattatca agcagatcca caggttgttg aagtttcagc aaagacatcc ctacttcgtt
     6601 tacgccatct tctggacgag catgaggtaa tgcgatgtga tcgccaatat ggataaacgc
     6661 accatactgc tccacgcgct cgatcatagc tttgatataa ctttcttcta ttttattttc
     6721 tgataatagc ggctgtgcgg caaatgtgat tgcttcttgc cagttttttg gttgatccga
     6781 taattggatc atttcttctg ttaataattc agatagcata ggacgttgat cctccttctt
     6841 ttctaaaagt ttgttcattt tacgatttaa tacttgatag agcttttctt cgtctacgcc
     6901 ttcttttaat tcgatataag gcagtagcgc cgacaacaat tcttgggcag aaggcattga
     6961 aaatcctggt atcagccaat ctctttgtac ttgattgatc agctgatttt tttccaagtc
     7021 agtcatgatt ggatgcagaa catagagaga ctttttctta tcagtaagtg tgagaggtac
     7081 agtagaaaaa ataatatcgt aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt
     7141 agtctctgta aaaaagatca tcgggaataa ttgctgaagt tcacttttta agatcaatga
     7201 agagctaatg ccacttggac agacgatcac ggctcttatt tttctatttc tttcttctgc
     7261 gtccgctttt ctgatctctc cgccaaatag tatagtgaaa aaaccaatct cttcttcagg
     7321 gatgctcttc cctattcgtc tttctaacgg aagtaatgct tttcttgtca tttcgtagat
     7381 ggaagcatat tgatgcttaa tttgatcgat catcacatta ggtaaataaa aagaatagga
     7441 aatacgaaaa taggcaggga ccaagtgata aaagacattc atcaataatt cacgaaacga
     7501 agcaaattct acggccatca ggaattccat gcgatggatg acttctgaag aacattccag
     7561 caaaaaatca agtgcaggct cctggacatc tccttcaacg atcgatgcta agcaaagaga
     7621 gaaaaatata atctccgaat tttgatgttc ggaatagcca aggccagtca atacttgtcg
     7681 actaatgtca gtgactgcaa gagaagccaa cagttcagca tcttttacca gttccatgga
     7741 tgtttttcca attcgaatgg ttgtcaaact caaataataa gctaattcat ccattcgatt
     7801 tggtgcgaat cgaagatctg atagtgaaat cgttttatct agcaaatctc taactttggc
     7861 gtaataatca atggaaaggg ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga
     7921 ctgcatcaaa cgagcagtgc gattttttgc ttctcttctt aatattgctt ctgaccctag
     7981 cagcctaaaa ccagttttcc tagtatacga cagctgacaa tcttccactt ctaattcatt
     8041 tcttatcttt ttgatatctg acaggactgt ccctttagaa acctgaagaa aatcttgaaa
     8101 atgaaaaaca gaaacagacg ttgcagtcga ataaaccatc aaataaagaa gatctcttcg
     8161 ctcttgttcg gtatacacag cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc
     8221 gattgctcgg aatttcttta tacggatacg ggtatcatcc acatattcgg caaaaggagt
     8281 taattgtttg atcgcctcgc ttgtatttac ttcgatgatc tctgctgctt tttctagact
     8341 gatttctctt ggtgttttca ggagataact aattttgtag ttcacagcgc tgttcacctc
     8401 ttacttatat tctagtatgg tagcgctttt aaatcgaggg gtgtttggta caacttacag
     8461 ttggattttt tttacgactg taccgaacga tttctatcga atggatacag aaaaaaagca
     8521 ggtgaccaaa tgtctcctgc ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt
     8581 tattttttga ggtcagttct tcaaattttt ctttgacatc ttcaaccgct ttttcgatat
     8641 ctttttcaaa gtgattgggc tcattaggct ttgtttcctc ttctacagaa tttgaaccag
     8701 ataaagctgt ttctttattt tctgtttcac cagagtttgt gccagctaat gattcttctt
     8761 tgtcttctgt ttcaccagaa ttagttcctg ctaaagccac ttcttcttct gttttaggtg
     8821 ccattgaacc tcttaatcgt ttgtgttttt caccgtcata atttgtcatg ttggttcctc
     8881 ctagtagttc tatacttttt catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc
     8941 cgattcttac aaatatgtca tatttttcct tctgattctt gaatgctttt gattccttct
     9001 tccaaaattt tcatttgtaa gattgtttct tcatctttga tgactttttc tgcgccatta
     9061 actaatgtct cgtaaatccc ttcgtagaca cggctgtaat caccaacctc ggaaattact
     9121 ttttcttcat gataactccc ttgttcatct atataggtca atacaccata atgttcgggc
     9181 aaatcgatac caaagtcttc atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt
     9241 cgatcttttg tttgtttcac gaacatccct ttttttccgt aaacgacgaa acttggacgt
     9301 tctttgatgc ggaaataact ggattttatc gaaattttca tatttccata gaaaaaatcc
     9361 agatcgaagt aatcattcat tcgtcctttc cctaaaagtt gacgtacatc atagctgata
     9421 tcatctggtt cgccgaaata agaaagaact tgatctactg tatgacaagc atgtccatat
     9481 aaatagctgt tcgcagtaga aaattcatgc gtattttctg gtacttccgg tctgaaataa
     9541 tcataatgca tttctacttc taaaatatcc cctaattttc cgctttcgat tactttttgg
     9601 actgtcaaga agtcagaatc gaaccgtctg ttttggtaac attggataaa tagacctttt
     9661 tcttttgcta atgcaaataa ctcttctgct tcctttgacg tttctgcaaa aggtttctct
     9721 actagtacat tcttaccatg ttcgagtgcc atttttccat attcataatg aaattgacta
     9781 ggtgtagtga tgacaactaa ttggatctca ggatcatccc aaagatcaga cagctgatca
     9841 gtatattcga tgtcttcgaa tgttttccac actggttcag atggggaaaa aatttttttg
     9901 atattgattt tgttcgtaag ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt
     9961 ccattgccaa gataagcgat tgtaagcatg agtggtgcct ccttttattg atgttttcag
    10021 tatatcctag aaaagcgcag aaagtattta taatagctat tctggtaaaa aagttcagaa
    10081 aaacacttgt tatcattcgg tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa
    10141 aagatagtag cggaaccaag tgtttatcag taagatagaa tttggaggtc agatcatgct
    10201 tattgaagaa aaactaacaa aacaagagtt gtttaccacg acagagaaac ggattgtcga
    10261 ttacatccgt aggaatatcg aagctgcagt ctacatgacg attgaagaat tagcaaaagc
    10321 tacgtatact tcccattcag ctatcattcg tttatgcaaa aaaatgggat tcagcggatt
    10381 taaagaattc cgtttcgagc tggggcggga agttcatcaa ttgattgctc agttcaatca
    10441 aacgattgat cctaattttc ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa
    10501 aatggcggat ttatcgatcc agtcaataaa aaaagcacaa ttgcagattg aacatgaaga
    10561 tttagatcaa atcggtaaag tattgaacaa ggcacagcga atctttcttt ttgcaaaagg
    10621 tgattctcaa atcacggcaa ggaaattcca gaataaaatg gtcaagttga ataagttttt
    10681 gatcatggct gaagaataca gtgattcttc ttggaatgca gccaatttga ctagcgagga
    10741 ttgcgccatt tttattagct acagcggtcg catccatcat tatgaacgaa taatgaccta
    10801 tttgaagcat gtagaggccc ctaccttgct gattaccgga aatcagcatt cagaaatggc
    10861 caaacaggct gatatgtgtc ttgttatatc gcaggaggaa tatgattttg ctaaagtagc
    10921 gactttctcc tctcaaattg ccttcgatta cgtattaaat acgttattct cagtgatata
    10981 ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa
    11041 aggccttctt aaagaatatc ggaaaactta agaaaagatc ctcatttagt aataagaaag
    11101 atagtgtttg ataggctctc tttgatatca tcataaaaac aggctgaagt tttctttttc
    11161 aaacctcagc ctgtttgttt acggaacgat tcgaatagat atattataga aatgaggaat
    11221 agctaacttc tgtttcttat tttgaacgat tgacgaaaaa gatatataag aaggaggaaa
    11281 atcagtatac atgcagctaa caatccatac aggaaaaatc gtttgttctc ggatactttc
    11341 tgtacttctt tttgaacttc ttttgcatga taaggaaccc ggtgccctct gacaagtaag
    11401 cgatgactgt tgaccatata aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt
    11461 tcgattctca gatcttttgt atcagtcggt tctaccgtct tgatctgatc gacttgataa
    11521 gcgagtgtcc gtccattgat ttcgatatag aaagaatcac ctaaagtcag tttcggtaag
    11581 tctgtgaaca actttgcttg aggcagtcct cgatggctgg aaataacggc atgtgtattt
    11641 tctccccccg taggataaga cgttccttcc aataatgagg cacctttttc tagcagcaca
    11701 gtattcgttt catcaaaaat tggcaaatgg acattgatct cagggatcgt caaaataccg
    11761 atcgtatgtt tctcaaaata ggttctatcc tttttcgcag gttctttttt cttagtaaag
    11821 ggatctgcac ctggatttcc accttctttt gctaattgct ggtttttctt tgtcatgttt
    11881 tcttggattt tagccatgac ttcttcattt tctttgaccg cttgttgctg ataatgagag
    11941 atgatctgct gatccagata attattcagc gcgtcgctaa caaatggata agctagtatc
    12001 cctattcctg agagaagcaa gaaaagcatc agcagatcta gaagtttcct tttctttggt
    12061 ttcatttgtg ttctccttgt gatgactgtt tgataataaa agaaaaagga gcatttgcgt
    12121 aatccgctgc tcctttttcc aattcttgca aaaagactaa atctgactgt gcttgcgtct
    12181 agcaaagtac agtccagcaa tcagtagaag gactactcct gcaccgatat acacatagat
    12241 tcccttaccg cctgttgaag gaagtgtacc tttgtgttta tttggtattt tttcaggaga
    12301 aatcaactgt cctgctgtta cataagattg ttcatcgata gtaaatgcta cacggtttgt
    12361 taatggaaca tatttttctg gcgctttcgt ttcttccaga tagtacgtgc catattttag
    12421 acctgtcaca tcgattaaac cgttacttgt ggttgtaaaa accgttgctg attctttcgc
    12481 cgatacccag ctgacggctt ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact
    12541 atcttgatca cgaacgacga attctgctcc agcaagtgtt tggtctgatg taacgtcacc
    12601 atctacttta acaaatcgtt tgcccccagt aacgacatcg actgacggtg gtgtttgatc
    12661 atttgtatgc ccgttatcga catttgcttg gttagaaaat cctttggttg gatctgcttt
    12721 ttcgttcaaa tgcatatagt aaacgaattt caatgtaccg ccaggagtta atgaaggaat
    12781 ataattcgga tcaactgaaa ccgtgaatcc gtctgtttgc tcagtgacag aataatttac
    12841 tgggtcgatt tctttatttc catcatataa ggcataagca tacgttcctg aagaatcatt
    12901 atcaaatgtt aaagcagcgt catgagtatc gataagtttg aatgttgtgt acttgttttg
    12961 cgttccttct ttatcagcga tacctaatgg gatattgaca gaaatctcat attgaatttt
    13021 ttcaccgatt gcgacatctt ttccattcaa ttgaggtgtt gttttatcaa ctttagacgt
    13081 atcatttttg atggtctttt ctactggtgt ttggctattt gccaggatct caaaaggcgt
    13141 gattgtttgc ttttcaatca tttccgcatt atcaggagct tttacttctt ctaaattata
    13201 ttttccaact tctaaatgat taacgatcaa ctggcctttt tcaatagatg cctcggcaaa
    13261 gtcattgttg ccgatgtcat aagaatgacc agtaatgaaa tgtttggctt tggtttgatc
    13321 agttgtccaa gtgtacaatc catctgtgac actttggatg tattttttga cgcttggtgt
    13381 tccttcttct ttagaaataa taaattctgc tccatttagt gcttcgtttt cggcagtacc
    13441 gatttttgta actttcaacg ttccatcatt accgactgta tttttagggt agagatggat
    13501 agtatctagt tcttctgtcc cgtatttata agagccatct gcttgtttga tcatctcata
    13561 tacagggaaa gctaaaacca tgtttgcggc agctgacact ccgtcttttg gttcttcttt
    13621 gatcgtgtag actgcatctt tcccattttg ttttttaggt aaagataaag tgacatttcc
    13681 atcagcatct gtcgttcctg aagcaactgg tgtaccagga gtcaaagact ggactgcttg
    13741 ttttgctgca tccacggacg ctcctttatc tcgttgcgca taaaattctt gagtgacgtt
    13801 ataaactgaa aatgaaatat cggctaatcc ttggtattga tcgaattcgc tcatttcttt
    13861 cccgctgttt tggattaaag gatcgggtaa atcagtcatt ttctttttat gaaggatgac
    13921 ttgagctgct gtctcctctg caaacacttt ttttgcgccg aagctgtttg tgagtaatgg
    13981 taaaataagg aaaaggactc ctaacataac gtttattttt ttatgatttt tcattttttc
    14041 actcctgttc tttttttgat aaacaaccag attgcaaagc ttgcgaatac cataccaaca
    14101 acgcctaaaa gtgtattttt cgtttcgttt gtttttggaa gaaatggacg gctattttct
    14161 tttttattga ctactttcaa tatactgtcc gcctctttat cctcagaaag aaaagttgaa
    14221 atggtaaacg gaatttctgt ttcacttaaa atataaccag aaggtgcttc gatctctttt
    14281 aatcgataac ttcctgtttt caacccggaa attgaaaaag cgccattttt atcggaaatc
    14341 aggtgaagcg catctgattc gttctttgtc cacttgtatc cgttggctgt ttgctggagg
    14401 taatttcctg ctttgttttt aataaggaat actgcgccag ctaatcgttt atctggttta
    14461 tttgtctcga ttttgacaaa ttgcttactc cctgtatata cagagacttt ctttgtactg
    14521 acaccaaaac caaaatccag tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta
    14581 ctgcttagct gcatctgata agaaatggtc agtttttttc cagcatattt ttccaacgaa
    14641 gggatcgaaa ataaaacatc aaatccgtga ggtttcttgt gtatgacaaa gccttctgtc
    14701 agcttttctc cattcgatga aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc
    14761 gcactatctg acaattcgaa cttttgatag tcaaggatat ttacaggaat tgtcgtatgc
    14821 aaggaataag tgattttttc accaatcgta aaatcatttc taggctcttc taataccttc
    14881 tcaaaaggtg gatcagggta gtcgttttct tcatttttag gatacaaatg gatagttgaa
    14941 agtttttgat tgttttgtcc atgtacagga agaacaacta ccatattctc tgccttttct
    15001 ttgacgactt ctggtacttt ggattcaatg aaaagatacg ctttatctct tttttcttga
    15061 tctttagcgg ccagagaaaa agaagcgata ccatcttcgt tgttaagagt tgtagttgtt
    15121 gcttctgcgg taaacatacc ggaagacgca ccgttttttg cgatctctgc ttgtgcttct
    15181 tctaccgttt tgcccttttc ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg
    15241 aatgtgacac catttaatcc tcgatacgtt tgtaataaag ctttttcttg tccgtcattt
    15301 ggatgatttt tcggcagttg cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc
    15361 atttctgttt ctgtttctgt tgctacctga gtaaaagctg gtttaaatag taacaagaag
    15421 agacacatac taagccaacc aagttttttc atatttagct gccccctctt tcttttttca
    15481 aaaaaagata ccaaatagaa aacgcacaac caatcatccc tactagatag attcctaaac
    15541 ggccaattcc tcccgtttca ggtaatggta cttttgcctg attcgtgatg tctaaagaaa
    15601 tctggttgtt tttggctcct ggtgacagaa cagattcatg atcttgtcca tccacttgaa
    15661 ttgacccatc ttcgtgtata actatagtaa ctggctcttt tagaccttgg tatccttctg
    15721 gtgtaaaagt ttcggtcagc gtataagttc caggttttaa attttcgaat agaaaggtat
    15781 ctgtttcttg tccatctttt ggcgattcta agtccatttc tggtccctgc agtctgaatt
    15841 ttgctccttt taatgtctgt ccgttgtctt cttttttgtg taccgttaga tcaaacgctt
    15901 ttagtcgatt ttgatgagtg agcgtccata cattatttgt catctcggta ttttttcccg
    15961 tgtaaatgat ttttccatat ttatcgattt ggaattcata atttcccggc gaagtatcat
    16021 atccggcagg accagctgtt tcaacgactc gatatttacc aggtgtgagt gaatcaaaac
    16081 tggcaagacc tttttcattc gttgtttggc tgtcaattgg ctgataagta ccatttgctt
    16141 cttttttctg tagggaaaat gctgctcctt taagatttat ctctttgtcc gtcccttgca
    16201 tcgcatactt acgtactgct acaggaactt caacaaaagg attttcaata gtcaactgga
    16261 tcgtatcatc ggaagtagtg actgtttttc catcaatggt taccgtacca tcagaagcga
    16321 tcttgatttc ccaagtagtc tttttgctta atccatgccc ttctggagct tttgtttctg
    16381 tcagcgtata ggtcatttct ttttgcaatt tgacattttc tggaagagaa taggttccgt
    16441 cgccatgatc tgttagtaaa gtatcaatag aatctcctgt taatttgaaa acagcgccaa
    16501 taagatcctt ttcacctgta gaggaatttt tcgttatttt caagtttaac ggtgtgaatt
    16561 gtttagtatt tttccatgtc attgcatcta tttgttgaga atcgtatcct ggtacaggta
    16621 attctttgat tgtttggtaa ctgaatgctt gaccttgatt attgtattga ggtagtgata
    16681 atatctcttg ataactttct ccgctatttg cagataattt gtcaatgtct gcacgttccc
    16741 acgtatttgt tgtatcttta gctggtttaa tgattcgaat atatccgttt ttccaagctg
    16801 cagcatttgt cgtatgttcc cgttgaatct caaaagtaac ttgatctgga cgatcagcta
    16861 gattgttgtc aaattcttcc cataactttt tgatgtgaag actgactccg ggagctttag
    16921 cagatggtat accgaattca gctaattcat tggtatcaat gtttggctgg aaagttgtcc
    16981 tgccgttcat ttgataccag aaatttggat ggaagtcctc attttctgtt tggattctca
    17041 cttggtaatg gatttggatt tcttggtctt ttcctaaata gatctgattg ctcttgatgg
    17101 tatttccttc tatggaaata gatggagtga ccgttgtagg acttgtcccc acactcttga
    17161 ctgaaagtgt accaggctga taaatgaatg gttctgcgat tggatcattt atttgtccat
    17221 tgcttacagt tgctgagatc tgtacagctt ttttggctag gtattcagag atatctgttg
    17281 catgatcagc tgattcatag taaagatcgc ctttttcatc tgatgaaacc atttgtcgca
    17341 tacgagactc tacttctgct tttgagagac cagctgccgg atcgctttga agttggatgc
    17401 caagaccatg tatttcgatt cctcgttctt tgagtgccat cgcttctccg atggttgcga
    17461 taaacgtact gtcgattctt ctggatagat tgttttggtc aggtgcatca tagattcttg
    17521 agattagaga agtatttccc ggccgatctt gcgtattaga aaactgagtt ccgtaataat
    17581 tgctgcttga ttgtgcgtgt acccgctgta ctttatagga aaatgttggt acaccatccg
    17641 tcagcaaaac gatcactttt ttatgtccat ttggaacgga tagcatgctt cctgcatctc
    17701 ttagtgcttt ttgtgtaaaa gtaccaccat ttgtccgtga aggtgtaatg gattttactt
    17761 gatttttcac tgaatcaaat gaacccatct gtactgcacc gttactgtag ctatatcctt
    17821 cgcttgagta gccgacatat cccatattga ttttgtctgt gataccgcta tctgctaaag
    17881 tatcgacaaa acgatcgaca ccaatcttta cttcaccgat ccgattattg tcgttcatac
    17941 ttcctgacca gtctacgacc aatacgagat caagaggcgt gatatctttt tgtacatttc
    18001 cacgagcatt caaataaaca tcaaacaatc cgggtgtact tgtttccttt gcatactttc
    18061 gtatcgcata gtctgcttga ttaccggttc ctccatattc gatatacgaa tgggtccgat
    18121 cattaggatc tccattccaa gaatttatac catcccattg atttgttccg tttttattgc
    18181 cttgatgatc aagaacattt gtatttcctg taggttgcca actggcttta ggataagtgc
    18241 cctgatcatc tgttgtatat tctgtaggaa tcgtttgatt agatggcaca ctcacagctg
    18301 gcaaggataa tgtaggcaat gtaaatgggc tgacagaatt ttcgttttct tttgatgatt
    18361 cttcttctat ttcgtctggc tttggtactg acacatctgt atctttctta ttttctgtct
    18421 catcaataat gtcatattct ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa
    18481 gtgctggatc gattggaaaa ctcagatcga tagtttgttt agtgtttgat gagatttcca
    18541 acgtaatttt cccgctattt acctgataag catagccatc tccttctttt ctttccacat
    18601 cacttaagta attttcgact agaccaattt ctttttctat cttttcagca ctggtgttat
    18661 tcgcatttag cttgatgttc agtgcttttt ttccagactg atccgaaatg acttcagctg
    18721 aaattgtttc taaaactgtt ggatcatctg tagtgtctgc tattgcagat aatggcacaa
    18781 agtttgataa actcaatatt atcagcatga aaataacttt cagtttcttc cctgtggtcg
    18841 tcatttttcc cctccttttt ctttttttct gttttgacgt gaatagtgaa aattattaaa
    18901 ataaacaggc gttacataat tttatttatt ttaatatgat aattattata cgaagggcgc
    18961 taaagggaat cacaccaatt ttcataccaa ttggtttcat ttatgaagtt gattccttcg
    19021 attaatgagc gtttcattga aagaaaaggg ttgagggaga aaaacatatg atcaggttgt
    19081 tggaaggaaa ctatagaaag atggaaaaaa tcattcgaat tctgtccatt tctgacaagt
    19141 ggatgactaa aaaagaactt gccaaacgaa ttggtagttc agaatctaca tttgtaaggt
    19201 atatcgaaga gatcaaaatc cgctggggaa cggatgtaac gattcaatct tcacaaaaat
    19261 tggggtatcg tcttgaacat ttgaatgtac cagcttattt aaatgttctg atggaaatgt
    19321 cgcaaagttc tacaaacagt cgtttattaa atgagattat tgtcaatccg ggtaaaacga
    19381 ttgagtatta ttgtgaaaaa atttccatca gccggtcttc ttttacacgt aagttgaaac
    19441 aatgcaatca aattttagaa agctatttat taaaagtgat cgttgaccaa ggttttcagt
    19501 taactagcct taaaagtgag ctccagttgc gtatctttgt tacctttttc tttttaagtt
    19561 actacggaag acgcacgttg ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg
    19621 tcaagaataa ttgtcagatg aatttgtttc aggaagaaag cccatatgag caatctttct
    19681 ttattatgta ttttgttgtg catctcatga gagaatccca aggaactcca tttgaatgga
    19741 aagctggaga ctcttatgta cagttgataa atatcgataa aaaagacttc ttaatcttga
    19801 aaaatctctt aggaaaattg aagtacagca gctatataaa agtagtgaac tattttgtca
    19861 atacgatttt ttcagaattt tcggagaaag aaaaaaatga aatcagtgag cagatacgaa
    19921 aaaatatgtc ctactgttcg ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg
    19981 ttgttcaatt attaacgcaa atttattttt tctcccgtat tttcccatac gatacgactc
    20041 atttgacgat ccgtgcaact tactttgcac agcagatgca acaccgccag atacgtctat
    20101 atgctggatt tcgtgcagaa cttcaatttt attccactct actgaatcag aatctgattt
    20161 attatttatc tagctatatc ttttggatgc ttatcggtaa tcctgaaatt caccattata
    20221 cttctgataa aaaaatactg attatcagcg acttaagtct tcgacatagt caatatattg
    20281 aagaatatat cagtgatatt cttgctgtgc ataaaattca ctctgagaca actgctatta
    20341 ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga tctgatcgtt acgaatcaac
    20401 ccattttgaa tgcccatgtt cctcatatat tgattgatga tagtgtgtca ttcgcaaatg
    20461 aagaagaact catccgctta ttcgagttat gaataatcaa aaaacagcta tgtataaacc
    20521 accctgataa aaatttcagg acaaatacac atagctgttt ctttttattg tattccttca
    20581 gcaatccaac gagatttata gcgaaagtct tttaacaact cttcttttgt ccaattattt
    20641 gcaatgagat ccgtcaaagt gttcattacg agggtcatgg aaaagtatag gaaactaact
    20701 gcttcttccg acgattgaat ggagaaatga ttgattctta ataattccag ccatttttcg
    20761 ttcattggag aatcagccga tagttctcta cgtttggttg tctgctgatc atttcgctgg
    20821 gttaacaagt ataacctttg ccaatagata ctttcgtgat ctaagtcgca gcaccactct
    20881 aaatattttt cgatccccaa aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata
    20941 tcttgatgga ctatgtgaga actgcttttg atcgtataca aataagcatc ttccaaatct
    21001 tgaaaatatt tataaaaagc accgcgcgac atccccatct tgtcaacgat ttctgagact
    21061 ttgacttggc tgatgtgctg tgagcaaaat atctccagta ataggtcatc gattctctgt
    21121 ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca tccacatctc tcctttatct
    21181 ctgattatac gtggatagcc cttcattgtc atgaaggatt cattccttct ttttctcttc
    21241 ttctgtcaag tacccatctt ccattcgata aacgcgatca ctccattgaa tcatacggac
    21301 atcatgagta accataatag tcgctttttg tttctcatga gcttctttaa ctagtaattt
    21361 gacgacttca tatgcatgat ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa
    21421 gcttggctca ttatataaag cgcgagcaat tgccactctc tgtctttctc caccagaaag
    21481 gtctttagga taactatttt taagtgactg gatatctaaa gattgcagca aggaatcgat
    21541 ttttgttgaa tcttcttttt ttgaagtaac ttggtcaact agttcaaatt gttcctttac
    21601 cgttaaaaaa ggaatcaaat tagagctttg gagaataaat cctatttctt taaagcgtaa
    21661 atctgctcgt tttttttctg gaagctcagt tagtgagtga ccgttgatca ctatttctcc
    21721 ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga
    21781 aggaccaatg atgctgacga attctccttc ctttacataa aaatcaattg ctttcaaagc
    21841 tgagaccttt gtatgaccag aaccaaatgt tttggtaact tttttcatcg ttaatatttt
    21901 tttcattttt attaacctcc tattgctgta attgggtcta ctttggttat cgtacgtatc
    21961 gaaaacaatc cgcctagcat tgcgacaatg atcaaaatcc cactatatag caaccattgt
    22021 gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca gactagtcaa ataagcaaac
    22081 aaaagagcaa gcagaacgcc taccacccca acaataaagg actgtgctac taaagattta
    22141 gcgatgaaga aatttttgat cccttgtgct ttcattactc caaaaatagc agtcttctga
    22201 agtgtcatca cgtacatgaa aataccgacc acggcagcaa ccactaagaa caaaaagtat
    22261 atcatcgcat ttaaggtcat attttgggca gaatagccag gaatactttc aatgaatgtc
    22321 ccaatagtta ataactgtag acccttcgat gctttttctt tcgaaatttt agctgaatcc
    22381 tttattacga tcccattgat cggctgttct tcttcagaag caaacggctg ttgtccatac
    22441 ttcaaagcag tccacgtatc tagggacgca tagatgacgg gagaaactgt gtaataagtt
    22501 tcaggaaaga taccgacaat cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct
    22561 ccaattttat agcctgaatc agccagattt tgagagatga tgatttcatt ctttgtttta
    22621 aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact
    22681 gtaacatttt ctttgttttt tcctttgatt gctccgctgt acaatccgat tggtgctttc
    22741 tctcctcctt caatgtattt cagatctcct cttgttaatt gtgaagctgc aaatacttga
    22801 tttgcatcct ccgacaaaac aatctcttgt gcgtcccaat catcgattgc ttttttaaac
    22861 tcttctgaaa gtccattcgc tagtcctgac aacatgaata cgacatacgc aatcaagaac
    22921 ataataccga cgatcaagcc atatcgtaat ttagaatagc gaatttcttt gatagctaga
    22981 aacatttgtt tttcctccct atgatagatt gatagtgaca aaatgtcact tgttgaatag
    23041 tgtaactcta aagtgacaat ttgtcacttg tgttgttttt ttcttatcaa taaattgtct
    23101 aagacatctt tcgataaaac cttaacatga gtcataaaaa atttatttaa gccttattta
    23161 tgctttccat gataaactaa agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt
    23221 agtagtagaa gacgataaag tattatccaa taatataaaa gaaatactcg ctgcaatcgg
    23281 cgagataaca caagtttatg atggtagtga aggactgttc gagttggaaa gtggagttta
    23341 tgatcttgca atcttggatt tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa
    23401 aatacggaaa gaaaaaatac agactccagt attgattttg actgcaaaag atagtttgga
    23461 agataagatt gaaggatttg aacgaggtgc agatgattat ctaacaaaac ctttctatcg
    23521 agaagaactg atcatgcgcg tgaaagcatt attgaaacgc tcgctaggtc tctttgatga
    23581 aaatgttctg aagtataagg atatcagttg tgatttaaaa agcaatgaag tcttgtataa
    23641 agaacaagtt ttgcccatac aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa
    23701 taagggaatc attttgacaa aagagcagat atttgaccgg atctggggat ttgattctga
    23761 tacgacgatt acagttattg aagtttatat gagcaactta cgaaaacatc tgcgaagtac
    23821 tggattggat aaagaaatca aaaccttacg taatgtcggt tatatactgc aaagggtgtg
    23881 atgcaatgaa tggagatatt acacgaaagc aacgtagtcg cttttttgcg acaaatgtag
    23941 cagcatttgc ttttatcttt tttcttttgg gattcattac tttacagata ttaaaccaat
    24001 ctgcatacag agaaacagat ctgtcattag agaaaataaa ttttgattct cgaacagtac
    24061 aattagagat acaaagatat cagcaaggcg atcccttttt aaacccgact caaacggaac
    24121 aaaacagaac aaacatggaa cagccattaa tggaaccatc aaatgaccgt tttaacactc
    24181 aagtgatctt gtggtctaaa aatggtgaga tattgaataa agcagctatt ggtgggcgtt
    24241 tcaaccaaat caaagaatta aaaatggata cgaaaaatct gaataccatc caaaatattg
    24301 aattaaatga agacagtggc tcatctgccc tctctttccg ttcgattacg aaaaaagcgg
    24361 agacgaatca agatggcgtt gcctacatcc aaattttaga aaatacaaat caaatcaaga
    24421 attcattagc gacttttcgc acgattatga ttttgtgtat gattgttttt tggattctct
    24481 ctattggtat cagttattac ttgtctagtc taagcatgcg ccctattatt ctttcttgga
    24541 gaagacaaaa agaatttgtt gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta
    24601 ttcaaaatag tttggaacat ctttttacca agcctaatca tactattatt gaagaatccg
    24661 aaagcatcgc tcaagcattg tcagagacga ggcgcctcac tggattgact tcggatttgc
    24721 tgacaatcgc tcggaatgat tctgatcagc agctgctttc aaagcaaatg atcaatacac
    24781 aagaatatat cgaacaactt gttaaaccat ttcaagagat ggccatcata gatggaaaag
    24841 agtttattct ccataacgat gcaacattag aagtaccaat tgatgaaaaa aaaatccatc
    24901 aagttttggt tattttgctg gataatgcat taaaatatac gaatccagaa gataagatca
    24961 ttttggattc atctgttaac cataaagaat ggctgattca cgtaaagaat actggccctt
    25021 ctatcagtga agaagatcaa gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc
    25081 gggcgaaaga gactggcgga tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac
    25141 agcatcatgg acagatttct gtatccaatt atcttccaca aggagtcgat tttaaagtcc
    25201 aattgccgtt gaagcctaaa aaataatgct tatccctaaa aaagggctgt gacaaaaatc
    25261 ttgtcacagc ccttttttgt tgcgaataaa cggtgttcca gaagtagctt cttcggaaat
    25321 aagccgaaat ttctttaagc tgaccacttc tgtcacaacc tctttagctt acgcttacga
    25381 ttttttattg cggtcttcaa tgtgtacatc accgcttgtg tcaatatcca attcttctcg
    25441 gctaacatct tctgtcactt tttcaacatc ttcatgttct tctttatgaa tcccaacttc
    25501 ttcacgaata actgtatgtt tgcttacatc tacttgctct tctttgattg ggatagtaat
    25561 cgtgtcatct tccaattttt cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg
    25621 ttcgatgacg atttcttcac gtttgaccgg tacttctacc gtttcagtat cgttgaccac
    25681 atgtttgtgg atgtcgactt ctccagttgt cacatcatgt gtatttacat ctaatttttc
    25741 ttcttttagt tgaattgtat catcatctag atttggtttg cgatcgatgt cttcagtctt
    25801 gcttgtaccc attgtatcag tctcgccagg tacgtccatc ggatcagttc ctggtgtcat
    25861 attcaatgga tccgttgtat tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt
    25921 tccagtgcca gtttctaatc cagtccccat tgtgcttgct gggctaacag tatcttgtgt
    25981 atccattgta tcttctgttg tttctggctg acgatagcct tttactgtga tgacaaattt
    26041 tccatcagaa atatctcctc tatattgata taaaggatca ttttctggtg tagccgtctc
    26101 agaatcatag gtatcaaaag aaaaagtatc ttttattttt tcccataaag aacggtcttc
    26161 ttcaccatcc atccgatttt catctgtttc aacatctata ccagacaatc cgtcaaatcc
    26221 tagtctagat gctgcttcat cagtagtata aataacgata tcatctcgtt catacccttc
    26281 gttacgcaac cgttcaatta ctgataatgc ttcttccctc gtactatagc ttccgatgac
    26341 cgttaaattt ttctcttcca tgtaaattcc tcctagcaaa tag
//
LOCUS       DAFGFZ010000031        25866 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000031 DAFGFZ010000000
VERSION     DAFGFZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.031"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(49..594)
                     /locus_tag="LR726_001106"
     CDS             complement(49..594)
                     /locus_tag="LR726_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL6216212.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(606..1298)
                     /locus_tag="LR726_001107"
     CDS             complement(606..1298)
                     /locus_tag="LR726_001107"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL6216213.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1317..1601)
                     /locus_tag="LR726_001108"
     CDS             complement(1317..1601)
                     /locus_tag="LR726_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216214.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1614..2177)
                     /locus_tag="LR726_001109"
     CDS             complement(1614..2177)
                     /locus_tag="LR726_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL6216215.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2405..3067
                     /locus_tag="LR726_001110"
     CDS             2405..3067
                     /locus_tag="LR726_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL6216216.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3191..4387
                     /locus_tag="LR726_001111"
     CDS             3191..4387
                     /locus_tag="LR726_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL6216217.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4488..5864)
                     /locus_tag="LR726_001112"
     CDS             complement(4488..5864)
                     /locus_tag="LR726_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6216218.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5901..6767)
                     /locus_tag="LR726_001113"
     CDS             complement(5901..6767)
                     /locus_tag="LR726_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL6216219.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6856..7881)
                     /locus_tag="LR726_001114"
     CDS             complement(6856..7881)
                     /locus_tag="LR726_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL6216220.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7899..8573)
                     /locus_tag="LR726_001115"
     CDS             complement(7899..8573)
                     /locus_tag="LR726_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6216221.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8757..9650)
                     /locus_tag="LR726_001116"
     CDS             complement(8757..9650)
                     /locus_tag="LR726_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL6216222.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9724..11421)
                     /locus_tag="LR726_001117"
     CDS             complement(9724..11421)
                     /locus_tag="LR726_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL6216223.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11414..12727)
                     /locus_tag="LR726_001118"
     CDS             complement(11414..12727)
                     /locus_tag="LR726_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL6216224.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12727..16764)
                     /locus_tag="LR726_001119"
     CDS             complement(12727..16764)
                     /locus_tag="LR726_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL6216225.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16777..17235)
                     /locus_tag="LR726_001120"
     CDS             complement(16777..17235)
                     /locus_tag="LR726_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL6216226.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17533..18051)
                     /locus_tag="LR726_001121"
     CDS             complement(17533..18051)
                     /locus_tag="LR726_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL6216227.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18053..18670)
                     /locus_tag="LR726_001122"
     CDS             complement(18053..18670)
                     /locus_tag="LR726_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL6216228.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18667..19530)
                     /locus_tag="LR726_001123"
     CDS             complement(18667..19530)
                     /locus_tag="LR726_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6216229.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19517..20428)
                     /locus_tag="LR726_001124"
     CDS             complement(19517..20428)
                     /locus_tag="LR726_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6216230.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20469..21803)
                     /locus_tag="LR726_001125"
     CDS             complement(20469..21803)
                     /locus_tag="LR726_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6216231.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22011..22841
                     /locus_tag="LR726_001126"
     CDS             22011..22841
                     /locus_tag="LR726_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL6216232.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23085..23367)
                     /locus_tag="LR726_001127"
                     /pseudo
     CDS             complement(23085..23367)
                     /locus_tag="LR726_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23525..23719)
                     /locus_tag="LR726_001128"
     CDS             complement(23525..23719)
                     /locus_tag="LR726_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216233.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24224..24409)
                     /locus_tag="LR726_001129"
     CDS             complement(24224..24409)
                     /locus_tag="LR726_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216234.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25259..25600)
                     /locus_tag="LR726_001130"
     CDS             complement(25259..25600)
                     /locus_tag="LR726_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL6216235.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25600..25830)
                     /locus_tag="LR726_001131"
     CDS             complement(25600..25830)
                     /locus_tag="LR726_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL6216236.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         7650 a         5543 c         4427 g         8246 t
ORIGIN      
        1 ttcttttatt attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc
       61 tgcgcctagt gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact
      121 tgccacggaa tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt
      181 atgcaacaca cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc
      241 tcgcaaacgg atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca
      301 ataaacattt tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc
      361 ccgtccaccc gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga
      421 atcttttgga tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt
      481 ctttgtatga gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt
      541 tccatcctct gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt
      601 cctccttata cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg
      661 tcttcgataa attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca
      721 cgtacgatca aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc
      781 atttcgcaag ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag
      841 tcgacaaatg tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt
      901 gctgctttaa ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct
      961 ggtaattgtc ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg
     1021 tctgatataa cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta
     1081 ttgatcacca tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag
     1141 acttttccta taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata
     1201 aataaagcgc ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt
     1261 acttcttctt ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat
     1321 ataaatgcga ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc
     1381 atcaaaaaag agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt
     1441 ggtttattct ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga
     1501 taagctgttt ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc
     1561 ttgcgaagtt cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt
     1621 cctttggtca atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac
     1681 tgatctttca tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg
     1741 tcttcatctt gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga
     1801 aggacttcat ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc
     1861 gcacgatagc cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct
     1921 tctcctggat cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga
     1981 aattgtttga caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca
     2041 ggatgaaata ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca
     2101 gcaacatcaa taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat
     2161 tgctcattgt tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca
     2221 tttttcaatt tttctcaaaa actccttatt agtttaccat agataaacct tagattttct
     2281 ctaaatatag aaaatccttc tttagtccga tactctttct agtagtccgt atgctaaaat
     2341 aagtatgtaa tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt
     2401 tattatgata aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt
     2461 ccgaaacaat agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt
     2521 gtggggactc tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac
     2581 tgacgaactt gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt
     2641 tcgacaaaca atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca
     2701 gacagccaaa cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt
     2761 attcaaagaa ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac
     2821 gcatctgccg aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat
     2881 caaaaacaaa cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc
     2941 agctttaatc gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt
     3001 agagatatct gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa
     3061 ggattaactc ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt
     3121 cgagtacttt ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga
     3181 ggaatttttt atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac
     3241 acttgaagat ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca
     3301 acaagcggag attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact
     3361 accggcggaa cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc
     3421 acagtctgtc atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc
     3481 gatgttgaat catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact
     3541 gatgtatcgt ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt
     3601 ccagcgtgtc ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa
     3661 gattggtgca caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct
     3721 aaaagataat ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact
     3781 gaatatttat atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc
     3841 agaagcgatg aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga
     3901 ttacacgcaa ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca
     3961 gatcacgatc cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc
     4021 tgaaaagatc caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat
     4081 cgcattgacc ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac
     4141 aacaaacgac ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc
     4201 gaaagaaaat gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt
     4261 gatcgaaaca atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca
     4321 tgcagaagta gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac
     4381 ccaatagatc acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact
     4441 gttgatgggc gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc
     4501 atcttgctgt gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata
     4561 ccgaacacga tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaagatg
     4621 gagacgattg gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca
     4681 aacatttttc tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg
     4741 atattcgtta gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc
     4801 acaccagcgg cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata
     4861 aacttaggaa gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc
     4921 atcgagatcg ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact
     4981 gttccatgtc ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta
     5041 tacggaacga ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt
     5101 atcgaaccaa tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc
     5161 attgcaacgg catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca
     5221 ccggaaaaac caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct
     5281 acaatgataa acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt
     5341 aatgctttct tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca
     5401 atcaagtcaa tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt
     5461 tcggccccta aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag
     5521 gaaaccgcta gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca
     5581 ccaaatacaa catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat
     5641 tctgcgaaac ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac
     5701 agtgctggac cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg
     5761 atgatcgcac caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca
     5821 ctcgtatgat tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat
     5881 tttaggttgg aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa
     5941 atgactaaaa ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca
     6001 aggatgaaaa tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca
     6061 acagctaaac caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat
     6121 aatccacaga tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag
     6181 aagcttgcgc ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa
     6241 ttcgcccagt tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct
     6301 ctaaaaccct taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct
     6361 tgtcgggcag tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca
     6421 taatctttcg tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt
     6481 aattgaaaac cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg
     6541 aactgtccat tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc
     6601 caaggggtga ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg
     6661 atcgtcattc ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca
     6721 atactacccc atgccaacat agggactaat gctattaaaa tttccataac caatctcctt
     6781 ttatttcaca tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc
     6841 gtttctaacg attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt
     6901 cattgttgtc ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc
     6961 cagatttttt gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt
     7021 tctcccagta aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc
     7081 tgaatcttta actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt
     7141 tccgtgatcc ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat
     7201 ccatttagca tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt
     7261 ttggataaaa taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata
     7321 ttcttggatg aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc
     7381 tacgtattca tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat
     7441 atcgaactcg atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg
     7501 gacccatgag tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac
     7561 ttcttggtgt tcacggatca gctgattgat atattcttct accagttttt cttcttctgt
     7621 cctcccacca ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc
     7681 ccaccatctt ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc
     7741 aatcaaagct aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat
     7801 cacaaagatc gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt
     7861 caaatattta aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt
     7921 cctacttttg tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga
     7981 ttgatgttga ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc
     8041 agcaattttt cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga
     8101 tattcatttt tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca
     8161 ttcaacgtaa tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa
     8221 cggcgaagta atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca
     8281 aaatcatctg cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag
     8341 ataaaaatga tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc
     8401 aaaacgggaa gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag
     8461 aacacttgat tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc
     8521 atgatcaatt gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat
     8581 ccctccatct ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct
     8641 tacctggaaa atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg
     8701 cgtttgtctc ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat
     8761 ctgttatttt tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa
     8821 agcgttagca ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt
     8881 tttgtctgtt ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact
     8941 agtaatcggt gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc
     9001 ttttctctaa ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact
     9061 tcataagcga gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct
     9121 tcaagatccg taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt
     9181 gtactctctc ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac
     9241 aaaagatcat tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg
     9301 gctccgatca tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct
     9361 tgctcaaaag gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc
     9421 tgatttttct tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca
     9481 agctgctttt gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct
     9541 aaaaatggat atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt
     9601 aataaaagac tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc
     9661 ttcttattta aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt
     9721 tgcttatact tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa
     9781 acttattccg attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc
     9841 gcgtttcata ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc
     9901 ttcattatag cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag
     9961 tacataacct tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg
    10021 aacctcaaag tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca
    10081 ctcaattcta tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat
    10141 tgcgtattca gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact
    10201 gcttgatgca tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc
    10261 tactccttct tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt
    10321 atggaaatca tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc
    10381 tcctccacgt gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc
    10441 tgtcaaaatc gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt
    10501 tgttaaaatc gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac
    10561 tgtaggcgta tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga
    10621 atcgatgaaa taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag
    10681 atcaatataa gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag
    10741 gtcgctttcc tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa
    10801 atgcacatct gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat
    10861 atcaggcatc atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc
    10921 tggcgaataa gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt
    10981 cacttggacg gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact
    11041 tgtcagtcga tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc
    11101 ttcggcagaa ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc
    11161 gtaatatact tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc
    11221 aaaatccgta gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt
    11281 tttgtgtaat gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag
    11341 ttttccgttc ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt
    11401 gacaatcatt tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac
    11461 ctaacaataa aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga
    11521 atacggacca ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt
    11581 ccgttgtttc ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga
    11641 ctggaacttt cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg
    11701 gttggccttt tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt
    11761 catccatctc gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca
    11821 agaaacgttg tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt
    11881 tgtcattcaa aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag
    11941 gagccatatc taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac
    12001 cttctaatgg ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg
    12061 gatctcttag ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg
    12121 attctgtcgg gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat
    12181 caacatccaa tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc
    12241 gatcttggtt tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg
    12301 tatctttccc agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct
    12361 gacggatatt gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt
    12421 tttcagcagc ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc
    12481 catttagtgg caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag
    12541 ccaagccatc attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc
    12601 gtttatgcaa cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg
    12661 gaagaaggac tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct
    12721 ttttcattac cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc
    12781 tgcaagcgcc atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt
    12841 agcaggcaac ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt
    12901 ctccaaaagt tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg
    12961 ataatacgaa atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt
    13021 ttctatgtca ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct
    13081 atagctgtca ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc
    13141 attgaatagg gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc
    13201 tcctgcacta ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat
    13261 actgtcaatt ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg
    13321 tagtacacca tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac
    13381 cgtatcttca atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga
    13441 tagccgatac ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg
    13501 cagaggttgg tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa
    13561 gtatccgatc ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc
    13621 tcgatacagc gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa
    13681 tgtatggcct tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta
    13741 agcagtgaat gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca
    13801 attttcttca cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa
    13861 gatccatgtg tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat
    13921 tttgtattct gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc
    13981 tccgattgga tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac
    14041 tgaatagaac gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt
    14101 cgctactgga tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc
    14161 gccattgatt ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt
    14221 gaattcattt cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt
    14281 aggctcttgt acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt
    14341 accttccggt ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc
    14401 taacatctca tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac
    14461 taactgataa cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc
    14521 agtttcatct cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt
    14581 tttacttgtt tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa
    14641 ttcgactcca gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc
    14701 tttatgttcg taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt
    14761 ctcataatcc tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc
    14821 ttgagctccc gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa
    14881 agaaatcgga gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc
    14941 cactacttct ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta
    15001 cttcatgccg ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc
    15061 tccaaatggt gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct
    15121 cttgaaggta gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt
    15181 gtaatatcgc ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg
    15241 tccaatagca tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga
    15301 gaagatactt ggcgggttct cttgatactt gtttttcagc caatgatgat aagcaactgt
    15361 gttgatcgca gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga
    15421 accatcacca acccagtgag tcgctttatt atctccttct tttacctctc cataacgata
    15481 gtgtacattt acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt
    15541 aaaccctgca ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt
    15601 atcttcagca atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt
    15661 acgataactt gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat
    15721 taggattttt tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc
    15781 atttttaagc cctagtgtaa gcggagttcc tccgattggt gtacgattta atgctgtatt
    15841 ctcacttccc gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg
    15901 ctcatggtta ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc
    15961 cagactcggg tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc
    16021 atttttagtt tgactattgg tcccagcggt taattcactc atactcgctg acttatctag
    16081 aacaaaaacg atatcgattg gactgatttc ctttttctcg cccccgatga catctagata
    16141 gacattgtaa agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag
    16201 aacaggttct acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa
    16261 attccgttca tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc
    16321 tgcagttgat ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt
    16381 ataatcacta tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata
    16441 gcgttcattg ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt
    16501 cgggtagcga tgctgttcgt tttcaacttc tccgctagga tagatatatt cataggggtc
    16561 ttgcgtagct gttggcccaa gacgctccaa aataccgttg aataactggc gttgaatgcg
    16621 ctggagtgct tgaccgctgg gtacgattgg ttcgtccagc ttttccatct cattttcttc
    16681 gctacgttga atttcagcgg aatcttctgt atcagtttca ttttcttgta attcttggtt
    16741 atctattctt tcatcaacca ccacttgaag aggtagttac ccttcgttca catcggggat
    16801 cttttctgtt tcaaaaatca atgtgccggt ttctgcttct tttgaataaa cttttccgac
    16861 ataccattct tgaccatcga tcgtctcgac ttttaggtct tctccatctt catcatagga
    16921 ggatagacct gttccacgaa cgttttttac ggtaccaatc ccggcggctg ccgcatcgat
    16981 tgctaaacgt attctacctt cattggcact ttcctttttt tcaaaagcca gatgccattc
    17041 aagggtttct tctttttcaa tatacttccc ttcaattttt gcatagtcct catctaagaa
    17101 tacctcatcc tcctttgttt ctgttgactc agcagctaca tccagggtcg tcatatgata
    17161 gactgactgg aaatatggtc caaatagcaa actgacagtt actaactgca aaagcttcaa
    17221 ttttaaccct ttcacatcct tatactcctt tcaaaaatat ttgtcttctt tcacaacaat
    17281 caacaaaaca atgcccatct aattataatt taatttaatt ttaattttaa atcaaatgat
    17341 atccttttta gttaaaaaac accaattata caacatttat tattaaaatt aaattataaa
    17401 attttataac gttctcattt aacaaaactt aagttattaa gacaaatgaa taaacaacta
    17461 cgctttcctc gaaacaaaaa aataaagcac aaatgcgcat ttacgcactc atgctttatt
    17521 cgtacaatta attcagataa cggcagcgca taggatgcca ataacacttc ctaaaatagc
    17581 tcctcctaaa atgtcagtag gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa
    17641 acaagcaaat aaaaaggcta atattcctgc aactggactt aattcgaaca acgcagctac
    17701 tgataaaaag gctagcgctg tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt
    17761 gtaaaggaca agccgacgat ttttttcata aggtcttttc cgtcttgtcg tttttttgat
    17821 aaaaagcaaa ccaacaaaaa aatagatcaa actgccgata agaatggcta gaccatattc
    17881 tctagtatca ggtatcaaga ctaaaacagc agcaaggagc aaccaaataa aaccaccatt
    17941 agagatatta ctgataaatt tcaaaaaatt gatttttcga ctatccgttt tccgttgctt
    18001 ggcgaacttg ttgataatac tatcatcgat tttagcaatc aaacgcaaca tgttattcct
    18061 cctttttaag aaccttttct tgcgtacgat ggataaaaag caggacttga aaataaaaca
    18121 agaaactgaa acctaataaa gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa
    18181 ggatcaacgt gcctgccaaa tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa
    18241 atgcagtaat ccaagaagct tctttttgtt ccagttgaag caagattttg taactaaaga
    18301 gaagcgcgct taatagaagc aaaaacaacg tagtatacat caatgtatag ccaattcccc
    18361 caccccaata gcggaaaatc tgataatcga tcaatagaag aagaaaataa acacttgcta
    18421 aactatcttg ctttccataa agagagaagt tctcacgaaa ctttttccag aatagtgaac
    18481 cgagcttttt ctcttcattt tttttgtaat gataaatcat atacagaact gttcgacttg
    18541 cagttcctaa tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt aaatggataa
    18601 gacgtggaat ccattcaatg atttctaaga aataatcatt ggctttttta ccaatcgatc
    18661 gattcatcat ccttttgaac ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc
    18721 aaaaaagaac agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca tcagataatt
    18781 ccattgtgtc gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc
    18841 ttgatcttgg atatataaca gaggacctaa gaaatcattc caagctgcgt tgaaggaatt
    18901 gatcgcaatg gtaatcaagg caggtttcgt caatggaagc atcaaatgac tccaaatata
    18961 caaatgattc gctccgtcga tttcagcagc ttcgatcaat tcattattga tcgataagta
    19021 aaattgacgc atcagaaata tattgaacgc attgccgaag aacgatggaa cgatcagggg
    19081 caaataagta cctacccagc cgatcttgga gaataataca tattgaggga tcatcgttac
    19141 aaaccccggg atcatcatcg tagaaagaac caaagcaaac atcagctttt tcccaggaaa
    19201 atcaagcttc gcaaatccat aagcaataaa agaatttgaa aggacattac cgatcactac
    19261 tagcacagtg atgaataatg tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt
    19321 atccagatag ttttcccata cgattttttc tgggaaaaag gtcggaggat aacgaacgat
    19381 ttcttccatt ggtttcaatg aagtaaacac catccaaagc aatggaatga tcaaaatgat
    19441 tcctattact gtcatgagga cataacttag tatttttttg tttgccgatt cacactggtc
    19501 tttttgaaat aatccatcat ttgccgtcac ctccagaata atagacccac ttaggtgcaa
    19561 gcttcaaatt gacaaacgtc agaaccaaca cgatcagaaa caggatcatt gacatagcca
    19621 tcgcataacc catatcaaaa gctacgaacg ccttgttcca catatgcaga ttataaaaaa
    19681 gcaaagaatt tgcaggtccg cctgttccat ttttcgtcat gacataagct tcttgaaaaa
    19741 tttggaaaga accgatcgta gaggtgatca catcgtaaaa aataatagga gtgattaatg
    19801 gcaaagtaat acttttgaac tttccccaaa cgcctgctcc atcgatttct gctgattcat
    19861 ataaagatct aggaacattt tgtaatgtag ctaagtaaag tagcattgct cctccagaac
    19921 tccatatttt catcaaaatc aacgcaggtt tcgtccattg tggatcaaac agccaagctg
    19981 gtccagggat atggaaccag cccagaaatg tgttgatcaa tcctgcagat ggagaaagca
    20041 gctgcatcca taagatgtag actgctactc ctgataagac tgccggtaaa tagaaagctg
    20101 tacggaatac actgattcct ttgatttttt gattgagtac tgtggctaaa aacacactga
    20161 aaatcgtcgt cagcggtaca ttgaagatga cgaaataaac ggtgttgtat aacgatttcc
    20221 aaaataaatc atcgtgaacg aacattcttt tgaaatttgc tagaccaatg aaattcatct
    20281 tggatgtcaa attataatcg gtgaaactac caataactga aaaaagcata ggccctagca
    20341 taaataccaa gaacccgata ataaaaggcg agataaataa ataggcccat cttgcttctt
    20401 ttttctttcg gatagataaa cgcttcaaat tttcttgcct cctctgtacc tagacagtca
    20461 aaaatggatt attgattttg cttgactaag ttttctactg ctttttgtgc atctgccaat
    20521 ccttcttttg gtgattttgt tcctaaaaga atctggtcaa tctgttcatt cactaatgtc
    20581 gtgtaatccg gagcatctaa aggaactggt gtgatcaccg tatttttaaa gttttcgtcc
    20641 gccatttgat agatcatttt tcctttatcc gttaggttgt cattattcac taaattctca
    20701 ttggctgtct tgctcgccat gatatcaaaa cttttttcgc cgaatttttc ttgtacttct
    20761 ggagaagaaa gatatttgat gaattcataa gaggcctcag gatcttttgc tccgtatggt
    20821 acttccaaga cgaatccgcc accccacgac caatggcctg aaccttcttc tttttctggg
    20881 agctgcatca cgccaaaatc aaaatcatcc ggtgcatttt ccatcaaact agagtaataa
    20941 ttgatatttt gggcacgcat ggctactaat ccggaaataa atggatcaga tacaccagac
    21001 ccaaattcgg cttctaattt attgatcgta tttttcccat aataatcttg ccagtcttgg
    21061 atccactcca aggcttccac tttattttta gtatcaatct tgactttttc atctttgtca
    21121 aaccaactag tcccgttatc tgcgtttagc gcccatacat cagcccctaa attccataat
    21181 ggataaaaac cgatgcgtgt aaattctttt ccttcttttt tatctagttt acgtgctgtt
    21241 tcttctaatt catcccaagt tgtcggtaga tcttgttcac tgattccagc ttctttaaat
    21301 atctttttat tataaaaaat gacttgtgta tctgtattga atgggatcgc ataaggatca
    21361 ccttcatatt ctactgtatc ccaaagctgc ggataaaaat ccttagaagt gtctttttct
    21421 acatatttgg agagattagt tgcttgctgt gcttctgctc tttgggcaac ggaatttatg
    21481 tcctgtacaa taacatcggg tgcatttcca gctgttattg cagataaaga tttagtccag
    21541 atgtcacccc atggctgata cttataattt acatggatct cgttttgtga ttgattgaaa
    21601 tcctcaatga tttcttcgat agcttctttt cgtgcaccgg atccccaaaa ggaccagaaa
    21661 tcaatatttt ttcttccatc acttgttttt tcagaagaat ttgctgcact tgaactggca
    21721 tcgcttgcat tacttgatcc gcatcctcct aataaaaaca atgctgaact tgccagcaac
    21781 cctgtaaaca ataatttttt catatttcac ctccaaattt taatgtaacc gatgccggtt
    21841 ttaatataac gatatcattt tgttctgaaa ataacaattt ataaggtatg attctaaaaa
    21901 aattattaat gataccaata ctttatataa ttcgcgagcg ctttctttga attttttaat
    21961 ctttcgtagc agaatgagaa tgtattaaaa atagattagg aggaattttg atgtttagac
    22021 accagaaaga attgcagttt gaagcaaaac cagatcgtcc gaatccatta atcgcgaaat
    22081 atctgcagga gctgatcggc ggccaatatg gcgagatgag tgtagctatg cagtacctgt
    22141 tccaaggctg gagctgccga ggagaagaaa aatataaaga tatgctgatg gacattgcaa
    22201 cagaagaaat cggccacgtg gaaatgttgg cgacaatggt tgctcgttta ttagagggtg
    22261 cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc aacaatcggt gccattatct
    22321 ctggaatgaa tccacagcac gcgatcgtag ctggcttagg acctcgtcct gctgacagcg
    22381 tgggcaatcc atggagcggt gcctatatca ttgcgagtgg caacttgtta gcagacttta
    22441 gagctaattt aaatgccgaa tcacaaggaa gacttcaagt agcacgtatt tacgaaatga
    22501 tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct tgcaagagat agctatcatc
    22561 aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa agaaggttca atcttagtcc
    22621 caagtacttt cccgcgtgac aaagaacgaa aagacattgc ttatgacttc tataacttct
    22681 cagagggaga ccaaagtagt gaaggttctt gggctagtgg caaagcactg gatggcgaag
    22741 gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc tgcaccaaaa ttagaaccag
    22801 taacaccaaa aatgtatggg acacctccat tcaagaaata aatatctacc cgtagaaaca
    22861 cccgtttttt gttccacacg ctttgtgagc aaaactgact aaagtcatta atcataacca
    22921 cccttcaaaa gggtggtttt ctctagagct ataagctcaa atcaccggcc agcgcctaaa
    22981 gacgctggct ttcatatttt atgttcaagc catattgcct ttgccagttt tgcccacctc
    23041 ttaaaaggtt tatgtactac tccttagccc ttaaaaggat cgtcttactc tcgtacactc
    23101 agcttatcta acgcactatc atgtttttct tgttcttgaa tgtacttttt gatggtcgct
    23161 tcattttgtc ctacttttgt cacatagtat ccttccaccc aaaagtgtcg atacccaaat
    23221 ttatatttta gatttgcaca atctcttgat gatctcctga atattggctc gatattgatt
    23281 ataaattatt tttcttctat actttggtgt gaatacaatg tggtatttac acatctactt
    23341 tgtacgggct aaactatttc cctttattgc tccacccgca tagcaagtgg ttttgtattt
    23401 caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa tctaaaaacc cgctgcttca
    23461 ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa aaagatctct tacggcaatc
    23521 tattttattt ttttgatttt ttataaatgg aaaatgcttc aagaagtcca agaaataaag
    23581 ccgtataaaa acaagaacct atgaaatctt tatttattag atattcaaca gaaatcccga
    23641 taacactagc caacaatata tttatcagaa gtgtaaaata aggatgcttt ttatagaata
    23701 gaactagtgt tgttttcata gactctcctt tcttcatatt tccaattaat cacaaattca
    23761 cggcattact agaaaaatta aaggggcgca gaagtggaaa aatactcttc gactttccct
    23821 acaaaccagg ccatcttgtg tttaataact ataccaaatc taaggtacct ctaaatctaa
    23881 attccatttg tggcggtgac ctccaatttt taactcttct atctcccatc cagtatacat
    23941 aaccttgata cttcgaccac ttatatatcg aacacacaaa tacctatcca ttgcaggagt
    24001 actacctaac ttagttgcta cttgcatcta aataacagct gaaaagatag gacttactct
    24061 tagtaaagta tctgaactat taatttttaa actatcattt ttatcctcaa ctacatgcac
    24121 atctgttgca acttcacttt ctgtctcatc cagataaatg attaaggaat cgggaattgc
    24181 aatatcttca gctgacgctg gaatagttga gctaaattag tcatcatatc tttattataa
    24241 aataaccaac agcaaaaaat acaatgaaaa acaaacttgg aactaatgta tcattaataa
    24301 atgttgaaaa attcagcaac tcattactgt atgattttag cagcccaacc aacatatacc
    24361 ctataaaagc taataagatg gttaggaatc ctttagaatt tttactcatt catagacact
    24421 tccttaaata acttttatta tagaaggtat tgtaaataat aatattgaca aaaagtgact
    24481 caaaataaaa aaaaccttac attatactgg aatgaactgt attgatagtg tacctaaaag
    24541 ctctactgac ataagatcgt gtaatcggta aagtaatttt attaattata cgggatgtaa
    24601 tcaattagag aataaactaa tcataaaagg tacttttaca ttcataattg tcgctttgag
    24661 attctacttt ggttcactat ctgacccaat tgcacatttt cgatcattac aagtattggt
    24721 gtcgttagaa caactaaata aaaactagcg gtagattttt cagaatctat gctaatttct
    24781 gtatggatgt tccagtgatg gcaaatttct cttttcattg tttttcttgt cgcatgatat
    24841 gcctttttgt gttattcagt taaactatag aaattctttc ctagacagat cttgatccca
    24901 aattttacgt ttttgagcat cgatatagtt taaaatgaca ttactcatat ctaaagagtt
    24961 tagatttttt aggactaaaa ctgtcagttg ttctttttct tctttctgtt aaagtattgt
    25021 cgcgactttt cgtatacgtt aagatatgtc gtgaaaaaac aagttttgtg acacaaaaaa
    25081 acgaatgact tgaggaaatc acctcaatca ttcgtttaaa atgctatata ttctttagtt
    25141 taagatacgg aaattaatgc catcgaccac actagtagaa tgaacgacat ttacttggcc
    25201 tttttttaat ttcgttatgt ttagcatata aaaatattat agagtttact tacaatgctt
    25261 aaaacatata ctgtataatc tgatcaattt tagccatgtc ttgcaatggt aagttttcta
    25321 tttttttgag ttttcgttcc ttaaaatcaa gagattttaa ttgtgtaata attacttgtc
    25381 cttttatatc tagctcttca ggtaatgagt aacgtgtcgg taaattcttt atagtggaag
    25441 taattggaca tatgaccgaa aacattgttt tacggttaaa ttcataacga gagacgacta
    25501 gtcctggtcg tcttttttga atttcttttc ctacggaggg atcaaaatca atccatacaa
    25561 tatcaccttt tttaggaata taaattcttt cttctgacac tataaaattt ctctcccttc
    25621 aggagttaaa tcttcccatt cgtctttctc ataatactcg gcctcttccc caccactaaa
    25681 tggatcttca atttttggaa ctaacgtaat tgttccatca tcagaataca caacgatata
    25741 ttcttggttt tctgaaggtt tctttccgtt atcagaaggt aaagttacca ctacagaact
    25801 tccttgtaat ctagattttg taacaagcat tatcatcact cccttaataa aagtatacta
    25861 tataac
//
LOCUS       DAFGFZ010000032        25192 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000032 DAFGFZ010000000
VERSION     DAFGFZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.032"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..746)
                     /locus_tag="LR726_001132"
     CDS             complement(<1..746)
                     /locus_tag="LR726_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL6216237.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
     gene            complement(987..3332)
                     /locus_tag="LR726_001133"
     CDS             complement(987..3332)
                     /locus_tag="LR726_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL6216238.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            3611..4513
                     /locus_tag="LR726_001134"
     CDS             3611..4513
                     /locus_tag="LR726_001134"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL6216239.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4724..6499
                     /locus_tag="LR726_001135"
     CDS             4724..6499
                     /locus_tag="LR726_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL6216240.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6565..8151)
                     /locus_tag="LR726_001136"
     CDS             complement(6565..8151)
                     /locus_tag="LR726_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL6216241.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8402..8626
                     /locus_tag="LR726_001137"
     CDS             8402..8626
                     /locus_tag="LR726_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL6216242.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8768..10519
                     /locus_tag="LR726_001138"
     CDS             8768..10519
                     /locus_tag="LR726_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6216243.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            10519..12303
                     /locus_tag="LR726_001139"
     CDS             10519..12303
                     /locus_tag="LR726_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216244.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            12638..13490
                     /locus_tag="LR726_001140"
                     /pseudo
     CDS             12638..13490
                     /locus_tag="LR726_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            13587..15473
                     /locus_tag="LR726_001141"
     CDS             13587..15473
                     /locus_tag="LR726_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL6216245.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(15514..16416)
                     /locus_tag="LR726_001142"
     CDS             complement(15514..16416)
                     /locus_tag="LR726_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL6216246.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            16646..18049
                     /locus_tag="LR726_001143"
     CDS             16646..18049
                     /locus_tag="LR726_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL6216247.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            18046..19482
                     /locus_tag="LR726_001144"
     CDS             18046..19482
                     /locus_tag="LR726_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL6216248.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            19627..20553
                     /gene="trxB"
                     /locus_tag="LR726_001145"
     CDS             19627..20553
                     /gene="trxB"
                     /locus_tag="LR726_001145"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL6216249.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            20766..22493
                     /locus_tag="LR726_001146"
     CDS             20766..22493
                     /locus_tag="LR726_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL6216250.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            22509..22859
                     /locus_tag="LR726_001147"
     CDS             22509..22859
                     /locus_tag="LR726_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL6216251.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22953..23813)
                     /locus_tag="LR726_001148"
     CDS             complement(22953..23813)
                     /locus_tag="LR726_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL6216252.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23905..24333
                     /locus_tag="LR726_001149"
     CDS             23905..24333
                     /locus_tag="LR726_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL6216253.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24495..24671
                     /locus_tag="LR726_001150"
     CDS             24495..24671
                     /locus_tag="LR726_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL6216254.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            24698..25144
                     /locus_tag="LR726_001151"
     CDS             24698..25144
                     /locus_tag="LR726_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL6216255.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7944 a         4499 c         5341 g         7408 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaatataa atcgcttctt tggatacggt ttgacgtttt
      241 agtggaatgt aagtagcgtc cataaaaata gcgacatact tatcatttaa ggctctggat
      301 ttaaaggcat ttacttcttc agtcagaact ttagtcatgt tggacatggt ttgtggagta
      361 tagtgatgac cgtacatttt ttcgatcaaa tcagcaattt cagacatcgt aacacctttt
      421 tcgaataaat ggataatagt ggtttccaat gtatcgtttg ttcttttgta ggctggtaaa
      481 gtttgttgtt taaactcacc attacgatct ctaggtattt ccaatgttaa ttcaccatat
      541 tcggttttga ttgatcgaaa gtaagaaccg tttctcgaat tacctgaatt aaaaccagtg
      601 cgatcatatt tttcgtaatc taaaaaagcc gttaattcag tccgtaggag tgtgtttatc
      661 gctttttcta agtgcgaacg gaataattca tttaaatcgc ctttagtgac tagagtttgc
      721 acaatttctg tagtaaaatc attcataggg aagtcctctt ttctgtgaat tggttgtcgt
      781 taactttatt ctacagaagg gacttccttt tttgtatgga ttttttcatt tacacaaaat
      841 attttatact ctctggtttt tcacattttt acataaattt gtcttaatat cgtagatgta
      901 agcttttgaa caccatttat tcggaaaaag ggagctgtga caagattttt gtcgcagctc
      961 cctttaattc tgattaatct attagatcaa tcatctttat cgtctgattc tttggtctct
     1021 ggattcgctc tagcataagc tgaagcggtg ttccagtaat ctgtatagtt ttcatcagta
     1081 ccgcctacgc cgaatttaaa ggcgcttttc ttcggaccat tttttgccca aagtgactcc
     1141 actgtttttc ctggcgtttg gatggatttt ttattgacag taacttttcc agctgtttgt
     1201 cctgtgaacc cagatacttt tttcttcatc acatcggatg ataagtcaaa tttctcatca
     1261 gtgccgaaaa tctcagggtt tgcttggtaa gcttgattga ttagattggc aagataatta
     1321 gccgaacgta ttcttgcttg gctgtccatt ccagtattgt catcgtgtcc tgcccaacta
     1381 ctgatagtga tagatggagt tgagacgatc agccatgaat ctcggtattc attggtagaa
     1441 ccagtttttc cgacccaatc tgcatttcct agatttccgt ttaagcttga gatgacatct
     1501 ttgaatggtg tagtgatttt ttcattgatc acactacgca tcatgtcatt catgatcgat
     1561 gccgttgctt cggaataaat acggacagaa ttactttcat gtttataaat cacattacct
     1621 gcattatctg tgatggaatc aatgatatag ccttcttcgt agactccacc atttgctaag
     1681 gcctggaatc catttgtctg ttgaagcgtc gttacatcta ctgtccctaa aggagccgat
     1741 tcaacgcccc aattatcatt tgctgggtag ttcatcttcg ccaagtagtg ctcgtaagcg
     1801 tattgtttcg atccaccgga attcaacaca tcttgatata agtgataagc tgggatattg
     1861 ttggaccatt ccagtgactc acggacggtt tggaaggtgt tcgacccttc attggtcgca
     1921 ttgacgattt tctcaccagc attttttcct tcttgccaag tcgttgcata atctgataca
     1981 cgggattctg atccgacaag tcccatatcg attgcaggac cgtaaacaag aaccggtttg
     2041 atggatgaac cagcttgacg ttctgtatca aaggcgtggt tattttgatt ctggctgtaa
     2101 ttccgaccac ccacgaagcc atagatacgt ccggttctgt tatccatcaa tacatttcca
     2161 gtctccactt ggctaccagc accatcatct aacatgtagc cataattttc tacgccggtt
     2221 tgtaatgcag catagatatc tttatcgatc gtcgaatgga tcgtatatcc tttgttttgg
     2281 atcgtttgct gtgctttttg ataataagct tcactgaccg atgaatctga catatcagca
     2341 ttatcttttt ctgccaattg ttgtgcaata atccttgttg cttcgttcat cacggtataa
     2401 tacaagaatt ctctgtcagt ctgttccgcg atttgttgtg ggagaaagtc ttttgtaaga
     2461 tcataggctt tcgcttcttc gtattgttct ttcgtaattt gtccttcacg gtacatactg
     2521 aataaaacaa aatcttttct ggcaagaccg gcagaaagat cttcttttag cgcgcctgta
     2581 ttcgtgtatg gactatacgt gatagggctt tgaggcaaac cagcgatata agcagcttgt
     2641 ggcagagtca catcctttgc atctacacca aagattcctc tagcagcttc ctggactcca
     2701 gcaatatttt gccccttatt attccgacca aatggtgaga cattcaaata cgtagtaatg
     2761 atctcatttt ttgaaaagtg tttttctact tgtgcagaaa gaacgatctc gtttgcttta
     2821 cgtttgaatg ttgtttcatc ggttaggatc tgttgtttga ctaattgctg tgtcaaagtg
     2881 gatcctccag acgagccgat gccagtcgct tctgaaacta gtgctcgtaa aacagcttta
     2941 gggacataac cttgatgttt atcgaagtat tcatcttctg tactgataat cgcggttttc
     3001 aataaaggcg aaatcttatc agatgaaata gtcgtccgca tcagatctgt ctggattttt
     3061 gatatctctg tattatctgc ataaacaatc tttgacgttt ccgtaatgtt tcctagttct
     3121 gtctgcagtt cttttttagt cggcaattgt gtgtcttcga caagataggc aaagtagcca
     3181 gcacctattc cgatacctag agctgcgaac aacaaaagaa gaacagtaat aaaaacagtc
     3241 aatgattgaa acactctaaa aataatctta ggaacaaacc atttttcttt ttttcttcta
     3301 ttctgttttt ttcgagtatt tttgcgtggc aaaagggtca cctcaactaa tggttttatt
     3361 tcgctaatta tagcaaataa taggacaaaa aaacagacaa ggctgttttt gacaggaatt
     3421 tctttttcga atgagataaa acgtgaggaa atgatctttt ttatgaaaga gttcttaagc
     3481 ttttctttag ggaaagtttc agttactatg gagaacaggg caaagaaaaa caaaccattt
     3541 tctttgtaca tcatcttatc aatatacagg aattgttcga aacaattgcc tttcaaggag
     3601 gaatccatcc atgttattag gctcacatgt cagcatgagc ggaaagaaaa tgctgttagg
     3661 cgcggcagaa gaagcagcca gttacggatc ttcgaccttt atgatctata caggcgctcc
     3721 tcaaaatact agaagaaaag ctatcgaaga gatgaatatt gatgccggaa aagcatttat
     3781 ggcagaaaac ggactaacaa atatcgtcgt tcatgcgcct tatatcatta atttgggaaa
     3841 tacgatcaaa ccagaaatgt ttccatttgc tgtacaattt ctgagagaag agattgtccg
     3901 tgcggaagct ttaggtgcta ctcaaatcac ccttcaccca ggcgctcatg taggagcagg
     3961 ggcagaagct gggattgcta gaattattga ggggttgaat gaagttttga caaaagaaca
     4021 gacggcacag atcgctttgg agacgatggc agggaaaggt acggaaatcg gtcgaacatt
     4081 tgaggaatta gcgcaaatca tcgatggagt aaaattgaat gaaaaattat ccgttacttt
     4141 tgatacatgt catacgaatg atgccggata taatataaaa gaagattttt caggtgtaat
     4201 ggaagagttt gataaggtca tcggattaga tcggcttaaa gtactgcata tcaatgattc
     4261 taaaaatcca caaggttctc ataaagatcg gcatgccaat atcggttttg gaacaattgg
     4321 atttgacaca ttgaacaaaa tcgtccatga tccaatgttt gacaacattt cgaaaatatt
     4381 agaaacccca tatgtcggta aagataaaaa gaaagcttac gcaccatatg gaaaagaaat
     4441 ttccatgttc aaagcgcaag cattcgatcc cgaagtgttc cctgagttat tagcagaaga
     4501 agtcacactt taagttttct acctgacagc aacaaaaaag gattgggcag tctgccttgt
     4561 ccttttttgt ttaagaaaca aaggagtgct ttttgcattc gttttcgcca tgctcctttt
     4621 gccccctctt tttaaattca gacaaacagg gtatactgat agaaaagtaa aaaacgaaaa
     4681 gtttcacaaa caagtagcaa aacgtgattg aaaggacgaa aagatgctga acattttaaa
     4741 ccccttgatc gaccaactaa aaaagaaaca tacatacttt attcttggca tagccgtatt
     4801 atttacaatt attcggttgt atttatatag taaagcaatc tatggaacag atatgtcggc
     4861 gacctatgat gatcagctgc tgattcacta tgctcaaaat attttggatg gtaaatggct
     4921 aggggagtat acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact
     4981 tgcacataaa ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag
     5041 tatattggtt gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat
     5101 ctatttttat tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata
     5161 tcgtaatgcc ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta
     5221 ttaccgcaaa tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc
     5281 ttttcctttc ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt
     5341 agctttgggt atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt
     5401 aaagaagtta aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt
     5461 gtttttactt cctatagttg gcttttccat cgtctatcag tggatcgaat ataaaaatga
     5521 gacaacatac ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat
     5581 gaaacaactg atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg
     5641 ggtctcacat gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc
     5701 tacccgtatt gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga
     5761 attacccgga gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta
     5821 ccaaaacaat gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga
     5881 agcctatcac aagggaacca tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga
     5941 tggaaagacg atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta
     6001 tgcatggcga tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca
     6061 agtcaaacag gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa
     6121 tgaaccgaga acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat
     6181 aaaaatttac caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact
     6241 tatgatacta ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat
     6301 tgtggtcacc ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg
     6361 ttcttatgcg ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc
     6421 attaacgcaa ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa
     6481 agctttatct tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg
     6541 gtctttgtgt ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg
     6601 ttcatatttt ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac
     6661 aagaacaatg attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg
     6721 ctcgccgaag aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact
     6781 aatcacagag aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat
     6841 ctccgcttgg ctgttggtca tcatcagtaa aggaatcatc gaaaatggca acgcaaaagc
     6901 caagaacacc tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc
     6961 ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac
     7021 cagtcttctt agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc
     7081 ggtcaacgta ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa
     7141 agtggacaag ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg
     7201 atcagataaa gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa
     7261 gacagcgaca cccatgatca atagcagtgc attgactaga aaagccattg acaactggat
     7321 attagaatcc caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc
     7381 gatcttccga gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc
     7441 accaataatc cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga
     7501 gaatgtatga ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa
     7561 gccaacttga tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc
     7621 gatcttacga aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat
     7681 aaagacggca atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat
     7741 gacttctgcg atgtctgttg ccataatggc taattcagtc aagatccata aaatgacacc
     7801 caatgtctta cttgtccgtg ctcggattgc ttgggccaaa tccatttggg tcacgattcc
     7861 taattttgct gccatatact gtaaaagcat agcaattaga ctagacatca aaataactga
     7921 catcaataaa tattggaaat tttgtccacc agtgatcgaa gtggaccagt tacctggatc
     7981 catataacca actgctacta atgcacctgg tccagagtaa gcaagaagtg ttttaaaaaa
     8041 actttttccc tttggtacat ccactgttcc atttacttca gctaaagata aaccatttgt
     8101 atgttcaata agtcgatgct tatgaaattg tttgttggat tgattattca agaaaaaacc
     8161 acctttccta tttatctacg attacattct acacctcaga aagaaaaaat taaagaaaaa
     8221 gattcggtat gcctaatttt ttttcgtggt gtgtcttgct ggttaatgaa agaaattttg
     8281 ttcttctcta aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt
     8341 caataaactt tagtaaaata aatcattgag agcaattcgg aaatagaaag gagagattta
     8401 tatggtatca acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt
     8461 aggtggcttt ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt
     8521 taatgaggac atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa
     8581 aattcgccag atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaaag
     8641 actttttgcg ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca
     8701 gactattcag caaaaaagac aagcacagac aatcctatgc tcttgataaa gacaaaggag
     8761 gagtaaaatg tcgattttta gaaaattagg ttggtttttt cgtcaggaaa agaaacatta
     8821 tttaattggg attttctccc tattcgcagt ggccttggta cagttagttc ctccaaaagt
     8881 gattgggatc atcattgatg agatcgccga taagaattta tcaataaaga tcatttcctg
     8941 gtggatctta ttattgatcc ttgcagcttt cttacaatat ctttttcgtt atatctggcg
     9001 gacgaatatt tggggaagtg cagctcggtt ggagagagaa cttcggcagc agctattcaa
     9061 acattttaca gaaatggaca atctctttta ccaaaaatat cggactggag atttgatggc
     9121 tcatgcaacg aatgatttga atgcaatcca gaatgtagca ggagctggaa tcttgacctt
     9181 tgcagattca gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg
     9241 gcgcttaaca ctgattgcgc tgattcctct gcctttgcta gccgttactt cacgagtgct
     9301 ggggtcaaaa ctccacgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga
     9361 taagacacaa gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga
     9421 agatctcgcg gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa
     9481 ttttttagac tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc
     9541 aacaattatt ttaggtggaa agtatgtctt ggaaggcacg atctcaatcg ggcagatggt
     9601 ctcatttatt agctatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt
     9661 caacgtattg gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa
     9721 gacgcatatc gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt
     9781 agctatttcc tcttttaact atccgaaaga agaatcacca gcattagaac aaatcaaatt
     9841 tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac
     9901 aatcatcaaa ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg
     9961 acgttcgatc aaagaatatt cattgaatgc tttattagga tcaatcggtt atgtgcctca
    10021 ggatcacttc ctgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact
    10081 ttcagaagaa gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga
    10141 attgccaaaa ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca
    10201 gaaacagagg atttcgattg ctcgagcatt gatcaaaaat cctgaattgc tgatcttaga
    10261 tgatgcactt tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga
    10321 aataagaaaa gagaaaacaa ccatcattgc agctcaccgt ttaagcagcg tgatgcatgc
    10381 aaaagaaatc attgttgtcg atcaagggaa aatcattgaa cgaggaaccc accacgagct
    10441 gcttgcatta ggtggctggt atgaacgaat gtgggaaaaa cagcaattag aagcgaagat
    10501 agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca
    10561 cttaaagaac agaagaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga
    10621 acgttcatcg ttgctatctt atttgcgttt gccctgtctg tgatcaatgt cctattgcca
    10681 aggatcatcc aaacttttat ggatgatcat ctggctaaac agtcagctac gacacaagtt
    10741 attttgtttt ttgctggtgt gtacttgttt ggagtcatag tcaaaagtat catttggttt
    10801 ttccaatggt atttgtattc catggcttca ttgaagacct atcagtatgt tcgggtaaga
    10861 ttatttgaaa agctgcatac actaggtatg cgttattttg accagacacc ggcaggatcg
    10921 accgtttctc gagtgacgaa tgacacagaa acgctatttg aattttggta tgtctttttg
    10981 atggtattga ctgggatctt tgcagtcgtt tcttctttcg ttgccatgtt tcaaatcaat
    11041 ggagaaattg ccttgtggac tttggtgttc ttgccgattt taggaatcgt catctggtat
    11101 taccagaaat tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac
    11161 acgaaattga atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct
    11221 cgtttagcta aagaatttga acaagtgaac aacgagtatt tagacacgcg ctatgcgatg
    11281 atcaaaacga attcgattct gttaagtcca atcatcaatt ttctctatgc attggcgatt
    11341 gcgttggcgt tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg
    11401 atctatgcat ttacgaccta tgtccaggaa ttttttaatc caatgtctca aatgatggac
    11461 tttttgagca cctttacaga tgggatggtt gccggtagtc gtattttgaa aatcatggat
    11521 acagaagaat atgcaccgca acaaaatcct ggagcagatc aagcgattac ttcaggtaag
    11581 atcgagtttc gcaatgtaag cttttcttat gatggagagc acaatgtatt gaacaatatc
    11641 tcttttgtcg ctaatccggg tgagaccgtt gcattagtgg ggcacaccgg aagcggaaaa
    11701 agctcgatca tcaacgtatt gatgcggttt tacgaatttt atgaaggcca aatcttgatt
    11761 gatgatcatg acattcgaga atatccaatg gaagagttac gtaagaaaat gggattggtc
    11821 ttgcaagatg ccttcttgtt ttatggagat attgcaggaa atatccggat gatgaatcca
    11881 gctatcacag acgaacagat tcgccatgca gctgagtttg ttcaagcaaa caagttcatc
    11941 gatgaattac caaaaggata tcactcaaaa gtgatcgaac acggtgccag ctattctagt
    12001 ggacaacgac agctgatcac ttttgcgcga acaatcgtga cagatcctaa aatactagta
    12061 ttggatgaag caacagcaaa tatcgatact gaaaccgaag gattgatcca agaaggattg
    12121 gcaaaaatgc gacaaggacg gacaacgata gcgattgccc atcggctgtc cacgattcgg
    12181 gatgcagaat tgattttagt tttagacaaa ggtcatatag tagagcgtgg gaatcatgaa
    12241 acacttctag cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtgtg
    12301 taaaaagagg ttgtgacagt agcatagtag cattttgccc taagcgctag agtgaaaata
    12361 aaacagagct tcgcagattc ttcatctgtg agcgttagtg gcgtagtcac ccgatttaaa
    12421 aaagacgacg acagctagtc aaaagcggct aataaccact aagaaagcgt tgacaaaagc
    12481 gtttgctttc gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag
    12541 ctaaacctga tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt
    12601 tggatacagc cctatatatc atggaaagga gcaaaacatg ctgattgaga aagtactgaa
    12661 taacaacgtc gtaataacca gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg
    12721 tttggcattc aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt
    12781 tcagttgaca aatgacgggc tttcacaaca gttccaagaa ttggcacaag cgattcctct
    12841 tgagtactta gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca
    12901 gttgaatgat tctgtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca
    12961 caaaatggga atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa
    13021 agagtatgcc atcgggaaaa aaaacagtga agaaaatctc ggataaatac cacatcaagt
    13081 tatcagaaga tgaagccgga tttatcgcat tgcatttagt caatgcacaa ggcattcaaa
    13141 gccaacaaga caatacctac ctgttcacta taacaataca ggatattcta aatatcgtcc
    13201 gctattacta taagattaca tttgatgaag attcagtcca tttttttcga ttcgtcacac
    13261 acttacgttt ttttctgaca cgcgtcctag atggagaaaa atcagaagtg ggagaagttt
    13321 ctgaggaatt gtttcaggtt attcaaaaaa aatataagga tacgtttggt tgcgttgaga
    13381 aaatagcaga ctttttagcc gagacgttca actaccagat gtccacggac gaaaaaatgt
    13441 atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa ataaaaaaag
    13501 gaggataaat taacaagaac gtatgaaata agggtagtga cattcagttg gctaaacctt
    13561 ccactaataa attggaggga atagaaatgg gaaaatacca agaattagcg agaaaaatcg
    13621 tagaaaatgt cggcgggaag gaaaatatca atggtttgac acattgtatc acaaggcttc
    13681 gtttcaaatt aaaaaacgaa gaaaaagcaa atgatgagat cttgaaaaat atggacggta
    13741 ttgtaacggt aatgcgagcg ggagggcagt atcaagttgt catcgggaat catgtgccag
    13801 tcgtgtttga agaggttata aaagcaggaa accttacttt tgatgaggct gtttcaacag
    13861 aaaaaatgag accgtttgat atgcttattg atggaatatc gggctgtttc caaccattcc
    13921 ttgctatcct agctgctgac gggatgattc gtggactgac agcattcctt gtcttcttag
    13981 gcgcgtttga ccgcggttct ggcacatttg ttatgtttga taatatcggt gattctgtat
    14041 tccaatttat gcctgttatt atcggtttaa cagctgcccg taaatttaaa gtaaatgaat
    14101 tcgtagggat gttgcttggc gcagcattaa tgaatcctag cttgtcgctg gaagctttga
    14161 gtggtgcagc agaagcacca ctaacgacca tattttctgg cactattttt gaagcaccta
    14221 tttatcaaac agtattcggc attccctgga ttgcgcgtaa ctacgcttct tctgtcattc
    14281 ccattatctt tattgtgttg ttagctagcc aaattcagaa accaattaaa aagttagtac
    14341 ctgagatgat cgcgaatttc ttcgttccat tcttcaccgt tcttattaca atgcctttag
    14401 gttttttact cgttggacca gtattcacat tcgcaactga tattttaatg gctggatttg
    14461 aaacactttt agcactttcg ccagttattt atggagcaat cgtaggcttc ttctggcaaa
    14521 ttctggtgat gtttggtctc cattgggcaa tcgtaccaat gggattgatg caattttctg
    14581 tgaatggttg gcaaaatatt atgacacctg ttgcagtagt gtcttttgga caaactgcag
    14641 cacttaccgc actctacttt aagcttagaa atccaaaaga caaagcaatt gcaataccag
    14701 ccattgtttc tggtattgtg gggattacag agccagctat ttatgggttt acattgccac
    14761 gtaaaaaaat atttattttt acctgtgtcg gtggtgcgtt agggggcgcg tatagtgggt
    14821 taatgaattt aacttcttgg aatcaaggtg gactaggcat ctttacaatt cccaactata
    14881 ttagaccaga tggtgattta acagatgtga ttaatgtatt gattgggatc gccattgcga
    14941 tggtggtatc ctttgcgctg acttttttct tttggaagga ggaagcagat gaaacagatg
    15001 atagacagaa aagatcaggt aaagaaattg taaaaacacc gatacaagga caaatcgctc
    15061 cactgaatgc agcaaaggat gcagcttttg cacaaggaac actaggtcga ggaatcttga
    15121 tctaccctga aaaaggagaa gtacgtgccc cttttgacgg aacagtcatg tccctgtttc
    15181 cgacgaaaca tgcaatcggg atggtttctg aagcaggtct ggaattgctg attcatgtcg
    15241 gattggatac tgttcagttg gaagggaaat attttgaaag cttggtgcag caaggagaaa
    15301 gagtgaaaca aggcgatctg ttgcttcgct tcgacaaaaa gaaaatcgaa gaggaaggat
    15361 acagtttaga aacacctgtg attgtcatca attacatgga ttatttggat attttggaaa
    15421 atcaagggga aacggtcggg ccagacgatg cacttatcac cgcactgacc tgaacggtta
    15481 ggtgaaagaa tggactaggc ttaactattc tttttagtcc acttctttat tttcaaaatt
    15541 gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcaaattttt gtcctacaga
    15601 aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat ataacacgtc
    15661 tagaagataa caaatcgatg tattgatcgt aaaattacca attttcgaat acagtcgttc
    15721 gcgtgtcgta agtcgcaaag cggctgtact cattgaagat agcgttgaat caccgatact
    15781 ggtcaaagct aaaattggca catgattttg tttcaatatt tctgcggtac gaaggatcac
    15841 attgttttca cccgtataag aaatcagaat cccacacata tcacttgtgc aattgtacgc
    15901 ttcatagaac gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat
    15961 tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt
    16021 ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag
    16081 tgtatcttga atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc
    16141 gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc
    16201 tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc
    16261 tacacggatg agtgtggacg ggtggacata acatcgctca gcaatctctt ggatggtcat
    16321 ttcgccaagt gctggagaat cgcttaagag aaactgaatc acttcatttt ctgaaggaga
    16381 aaaggagatt tctttgattt tttcacgtaa taacatgtag ataccttctt cttgtttttt
    16441 tacgtaaatc ataacttttt tgaaaatgat ttgtaaacgg atttatgtta tatagcttaa
    16501 taaagacatt ttttgacaag atatttacaa aaatccagaa gaaaacacta acttttgtaa
    16561 atgttactgg aaagaaacga atttctattg tttgttttgt ttacaaaaaa gataataaaa
    16621 gtgtaaaaag taaaggagtg agaacatggc aaaaggaatc aaaatcgcta cgattggtgg
    16681 aggcagcagt tacacccccg agttgatgga aggatttatc aaaagatatg aggaattacc
    16741 gatacgagaa atctggctcg tagatgtaga agaaggaaaa gaaaagcttg agatcgtcag
    16801 tcagatgtct cagcgtatgt gggatgcttc tccgtatgaa gtcaaaatct atgcaactct
    16861 ggatcgggaa gaagcattga aagatgcgga ttttgttacg actcagtttc gtgtcggtca
    16921 attagatgca cgaatcaaag acgagcggat ccctagttat tatgggatga ttgggcaaga
    16981 aacaaatggt gctggcggga tattcaaagc ttttcgtacc gttccagtgg ttttggcaat
    17041 cgttgaagac atgaagcgtt tatgtccaaa tgcttggctg attaatttcg cgaacccaag
    17101 cgggatggtg acagaagcag ttatcagata tggcaaatgg gataaagtga tcggattatg
    17161 caatgtgcca gtaatggcga tgatgacaga accaacaatg cttggcaaga aaaaagaaga
    17221 gctgatctac aagtttgccg gtctaaatca cttccattgg cacaaagtag cagataataa
    17281 aggaaatgac cgcacggcag aattgattga tcgtttatac gatgaagaca atggattgcc
    17341 gaaaaatatc tttgacatcc cgtttttcaa agaacaatta aaacaaatga acatgatccc
    17401 ttgtggctac caccgctatt actatcgtga agaagaaatg ctggaacatg ctttagaaga
    17461 atatcaaacg attggtacaa gagcgcagca agtgaaagaa acagaagcag agctgtttga
    17521 attatacaaa gatccagaat tggatcataa acctgaacaa ttgcaaaaac gaggtggtgc
    17581 ttattattca gatgctgcct gtgaaacgat tgcttcgatc tatggaaata aagaaacaca
    17641 gatcgtggta tctacaaaga atgatggagc tgttcctgat ctgccggcag attgtgtagt
    17701 agaagtcact gcctatgttg gcgcacaagg cgcacgaaat gtagcgtttg gttcccttcc
    17761 tgctgctgaa agaggctggt tgcaagtaat gaagaatatg gaattgctga cgatcgaagc
    17821 agccgtgact ggtgattatg gaacagcttt gcaagcgttt acgatcaatc cgcttatacg
    17881 atcaggaaag acagcaaaac ggatcatgga tgagttgttt attgcacatc agcagtattt
    17941 accgcaattc aaggaaacaa tcagccggtt aaaagcagaa ggaatcgaag tccaagatga
    18001 actggcaaga gaattatcaa gcaatacaca tgtaatggag gaagtatgat gggattcaga
    18061 aaagattttt tatggggcgg agctacagca gccaatcaat gcgagggcgg ttatgatgaa
    18121 ggcggacgtg ggttagcgaa cgtagatgtt gtgccaatcg gagaagaccg ttttcctgtc
    18181 attactggaa aaaagaaaat gtttgaattt gatgaaaatc atttttatcc agcaaaaatt
    18241 gcgatcgata tgtatcatca ttataaggaa gatattgctt tatttgcaga aatggggttc
    18301 aagacttatc ggatgtccat tgcttggagt cgtatttttc caatgggcga tgaaaaagag
    18361 ccaaatgaat ctgggttgaa attttatgag gatgtcttta aagaatgtca aaagtacggg
    18421 atcgagccgc ttgtgacgat tacacatttt gattgtccga tgcatttgat caaagaatat
    18481 ggtgcgtggc gttccagaga actcgttggt ttttatgaaa atctgtgccg agtcatcttc
    18541 aatcgctata aaggacttgt caaatattgg ttgacattca atgagatcaa tatgatcctt
    18601 catgcaccat tcatgggcgc agggctgtat tttgaagaag gagaaaatga agaacaaatc
    18661 aaatatcaag cggcacatca tgagttggtt gcaagtgcca tcgcaacgaa gatcgctcat
    18721 gaagtagatc cggagaacaa agtaggctgt atgctggcag caggagcata ttatccatac
    18781 acttgcaatc ctgaagatgt ttgggcttct agaaagtcag atcgagaaaa ttatttcttc
    18841 attgatgtcc agtcacgagg agaatatccc gcttatgcat tgaaagaaat ggaacgaaat
    18901 ggcattcaga tcaaaatgga acctggggat gccgcattgc tgaaagaaca tactgttgat
    18961 tttatctcct tttcttatta ttcttctcga gtttcgacta ctgacaacga actcttggaa
    19021 cagactgcag gaaatatctt tgcttctgtc aaaaatccat atctggaagc aagtgaatgg
    19081 ggttggcaaa ttgatccatt aggcttgaga ataacgatga atgatattta tgatcgttat
    19141 caaaaaccac tatttattgt agaaaacggc ttaggtgcag tcgatacgcc cgatgagaat
    19201 ggttatgtgg aagatgatta tcgaatcgac tatctggcag cacatatcca ggcaatgaaa
    19261 gatgcagtag aattagatgg agtcgatcta ttaggctata cgacttgggg atgtattgat
    19321 ttagtatcag ctggtacagg tgagatgaag aaaaggtacg gattcattta tgtcgatcgt
    19381 gacaacgaag gaaatggcac attagcccgt tcgaagaaga aatcattcaa ctggtataaa
    19441 aaagtaattg attcaaatgg agaaaatctt tcgaacgatt gatcaatgat ttataaaagt
    19501 ctattgtttt tcagcggatt tttcgagtat aataaagcta ttcgagaaac gcaagcagaa
    19561 caatagactt tttttcgcat tggctgtaaa gtatgcttac ttgattaagt aaaggagaat
    19621 ctacatatgt atgatgtaat cgttattggc gcagggcctg ctgggatgac tgctgcttta
    19681 tatgcttcac gttcaaactt atccgtattg atgatcgaac aaggcgctcc tggcggccaa
    19741 atgaacaata cggcagaaat cgaaaactat cctggtttcg attcaatcat gggtcctgat
    19801 ctagcttata agatgtatga aggtgtttca agatttggga cagaaaatgc ttatggaatc
    19861 gtgcaagata tcaaggatca cggcagccac aaagaagtta tttgtgaaga taaaacttac
    19921 gaaggaaaaa cagtcattat cgcaacaggc tgtatccacc gtaaattagg cgtacctggc
    19981 gaggaagaat ttgctggacg tggcgtttct tactgtgcag tttgtgatgg tgcatttttc
    20041 cgtaacaaac gtctagtagt gatcggagga ggagactcag cagtagaaga agccatctat
    20101 ttgacacaat ttgcttctga agtagtcatc gtccaccgta gagatgaatt acgtgcacaa
    20161 aagatcatcc aagaccgtgc atttgcgaat gaaaaaattt cctttatgtg ggatacagtc
    20221 gttgacgaaa tcgttggaaa tgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa
    20281 actggcaatg agtttgattt ggcagcagac ggagtattca tctatgtagg attggatcca
    20341 ttaactgcac catttagaga agctgggttg acgaacgaag ccggctggat tccaacagac
    20401 aacgaaatga agacgaatat accaggcgtt tttgccatcg gagatgttcg tgaaaaagat
    20461 ttgcgacaaa tcactactgc tgttggtgat ggtgggattg ctggacaaca agtgtataaa
    20521 tacttggaat ctttagaaga aacaactgca taagtttaaa aagagtgaaa gaacggtttc
    20581 aattgttctt ttactctttt ttttgcgtga aatctactga atccgattta tgcgaatact
    20641 tgtaaccgtg cttgataaaa gaatcttttc ttgatttaag cgttttttgg tcctcatact
    20701 ttcatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa agaggtgtta
    20761 ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa
    20821 acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa gatgcttttt
    20881 atgcaccgtt ggaattcggt actgcaggca tgcggggaat cttaggacct gggatcaatc
    20941 gaatgaacat ctatacggta agacaagcaa cagaaggact tgcacgtttc atggatacac
    21001 aaggtgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc catcaatcac
    21061 cagaatttgc catggaagca gccaaaacat tagcacatca caatatccct tcttatgtat
    21121 tcgaaagttt acgtccaaca ccagagcttt cttttgcagt ccgtcatttg aagacgtttg
    21181 cgggaatcat ggtaactgca tcccacaatc cagcagctta caacggatat aaagtctacg
    21241 gagaagatgg gggacaaatg cctcctgcag acgcagatgc attgacgaaa tatgtccgtg
    21301 aagtcagcaa tccgttaaaa gttgaagtat tatctgacga cgaagctgaa catagcggat
    21361 tgatcacgat catcggagaa gaagtggatg ccgcttatct agaagaaatc aaagcagtaa
    21421 cgattgatca tgaattggtg gaaacaatgg gtaaagattt gaagcttgtg tacacaccac
    21481 ttcacggaac aggaaaaatg ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat
    21541 tcgtactagt tccagaacaa gctgtcgcag acccggattt cactacagtc aaatcaccga
    21601 atccagaaga acactctgct ttcgagtatg cgatgcgact tggcgagaaa gaaggcgctg
    21661 acttactgat tgcaacggat cctgatgcag accgtttagg cgcagccgtt cgtttgccaa
    21721 atggctccta tgaagtattg acaggaaacc aaatcggagc cctgtttgct caatatattt
    21781 tggaagcaca ccaatcgcga ggcactttac ctgaaaatgc agcggtattg aaatcaatcg
    21841 tttctagcga attgcctaca gcaattgcgg aacactatgg ggctaccatg ttcaatgtat
    21901 tgacaggatt caaattcatt gctgaaaaaa ttcaacaatt tgaagaagat cattcccata
    21961 cttttatgtt cggattcgaa gaaagttacg gttatttagt taaacctttc gttcgcgaca
    22021 aagacgcgat tcaagcattg ctcttattag cagaagtagc agcttactat aaaaaacaag
    22081 gcaaaacatt gtatgacggc ttgcaaacga tttttgaaac atatggctac tacgcagaaa
    22141 aaacgatttc tgtgacaatg agcggacaag aaggctctgc aaaaatctct gctttgatgg
    22201 aaaaattccg tgaagaagca ccgacagaat ttgccggagc aaaagtcatc caaacagaag
    22261 acttcaaaga gttgacgaga acagaccgtg aaggaaacgt agaaaaacta acgacaccgc
    22321 catctgacgt attgaagtat acgctggaag aaaatggctg gatcgctatc cgtccttcag
    22381 gaactgagcc gaaaatcaaa ttttatatcg gtgtaaaagg aaactcttca gaagacgcag
    22441 ctgaaaaagt agccgcttat gaagcgatta tcaaaaaaat gacagaagaa taaaaaaact
    22501 tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag ttggaggtga
    22561 atggattgcc agacacgcaa aaaacaaaag tagagaaagt cagccaacta ttcaaaatgt
    22621 tgagtgaccc cactcgtctc aaaattctgc tgtatctgaa agacggggaa caaaatgtga
    22681 cagcaatcac ccaagcagta gaaatggaac aatctgctgt atcgcatcaa ctgcgtttat
    22741 taagagaaaa ccatgtcgtc aagtcccatc gtgagggaaa agcgattttg tatagtcttg
    22801 atgattctca cgtactggat attttgaacc agacgctcaa acatgtggaa catcaataac
    22861 gataggtgca aatagagaca aactggactg tatcaacgaa tcttcttcgc tgacacagtc
    22921 cagttttttt aagaaaaaga caagaaaaac tgttagtctg tttcttcggt ggtttcagtt
    22981 ccataataac tatgatcttc taggtctaaa gcattcaaaa taagtgaagc tgtttcttcg
    23041 atagataaag aagcgacatt gattactacg cagcccaatt tttcataaag atcattcgca
    23101 aaatcgagtt cagctcgtat tttttcgata tcggaataag cagtatcagg atttaatccg
    23161 taagagcgca tccgttcttt acggatattg tttaaaatat ctgggttatt cgtcagtccc
    23221 acgatttttt taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg tgggatcaaa
    23281 ggtagattgg caacctttaa atttttgtta gctaaaaaca agctcaaagg cgttttggaa
    23341 gttctggata cgcctagcaa aacgacatct gcttctagaa acccacgagg atctttcccg
    23401 tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt ctcattcaat
    23461 aaatgaaggg cgccaggttg ccgaatgggc ttgataccag ttaagcgctc gatttctgat
    23521 atcggcggag tcatcacatc aaaactaaat aattgatgtt cttcacaaaa agaacgcgtc
    23581 atctcgatca attcttgatt gatcaatgtg tgtaggacaa gggctttttc ttttagtgca
    23641 tcttgcagtg cctgcatcaa tgttttctca tctgtcacaa atgttctgcg gaataaattg
    23701 aactcaacgg taggatattg ggccatagtg gcttgcgcta attttgtcgc agtttctcca
    23761 gcagagtcag agatgacaaa aaaagtaatg atttctgtag aatcggaaga catggataaa
    23821 acctcacttg catttttttt atgtttatta taccatactc tagaatgtgt acttcgtaat
    23881 cattttgatt tggaggaata agtcatgaaa aaacagacat tgattgaaca atcactagaa
    23941 acattgaaaa ataacggttt taaatatact aaaaaaagag aagcattgtt aacttattta
    24001 gtgaagcgca atcgctacgt atccgctaaa gaagtttttg actatatgaa tcaggaattc
    24061 aaaggagtaa gttacgatac gatttatcga aatctccatg attttgcaga aatcgattta
    24121 ctggaagaaa ccgatttaaa tggagagatg aagtttcgtt ttcattgctg tcaaggggaa
    24181 atcggccatc accatcactt tatctgtacg gtgtgcggaa caacgaaaga aatccatatg
    24241 tgtccaatga attatttcga agaacagctc ggtggatgta cgatcgaagg gcatagattc
    24301 gagattttcg gaagatgtga aaattgtcaa taaggattta aataaaataa catttgataa
    24361 ttttaaaaaa aagcaagatt tccttgacgg tcaaaacagc attagatata atatttacag
    24421 gacagtcttg ttttttgaga tatatttcaa taacaattac ggttactttt taagctcgga
    24481 gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt gacgatgctc
    24541 ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt acaagaatct cgtaaacgtg
    24601 aattctacga aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct agaaaacgca
    24661 aaaaattcta gttctgtgat gaattggagt tggtagtgtg tcactactaa gtagattaaa
    24721 cgatgacatg aaaacagcga tgaaagcgaa agataaggaa tctttacaag tcatccgaat
    24781 gatcaagtca tctatacaaa acgagcagat taaagaaggt cacgacttga cagaagaaga
    24841 agaattaacg gtgttgtctc gcgaaatgaa acagcgacgt gattctttgc atgagtttga
    24901 agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtga tagtcgaaaa
    24961 atacatgccg gaacaacttt ccgacgaaga aattcgacaa cttgttcaag aagcaatcac
    25021 acaaactggt gcttcatcaa tgaaagaatt tggcaaagtc atgggtgcga tcatgcctaa
    25081 agtcaaagga aaagcagatg gaaaccaagt aaatgccatt gtgaaagaac tattacaatc
    25141 ttaattttat catttaaagg ctcttcgtca atgagtgttg gtgaagaaat tc
//
LOCUS       DAFGFZ010000033        24849 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000033 DAFGFZ010000000
VERSION     DAFGFZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.033"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(104..1336)
                     /locus_tag="LR726_001152"
     CDS             complement(104..1336)
                     /locus_tag="LR726_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL6216256.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="LR726_001153"
     CDS             1591..2160
                     /locus_tag="LR726_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6216257.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="LR726_001154"
     CDS             complement(2338..2778)
                     /locus_tag="LR726_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL6216258.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="LR726_001155"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="LR726_001155"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL6216259.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="LR726_001156"
     CDS             3732..4655
                     /locus_tag="LR726_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL6216260.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="LR726_001157"
     CDS             4731..5870
                     /locus_tag="LR726_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL6216261.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="LR726_001158"
     CDS             5863..6663
                     /locus_tag="LR726_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL6216262.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="LR726_001159"
     CDS             6663..7490
                     /locus_tag="LR726_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL6216263.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="LR726_001160"
     CDS             7468..8202
                     /locus_tag="LR726_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL6216264.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="LR726_001161"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="LR726_001161"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL6216265.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="LR726_001162"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="LR726_001162"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL6216266.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="LR726_001163"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="LR726_001163"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL6216267.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="LR726_001164"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="LR726_001164"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL6216268.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="LR726_001165"
     CDS             11787..13820
                     /locus_tag="LR726_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216269.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="LR726_001166"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="LR726_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="LR726_001167"
     CDS             15353..16159
                     /locus_tag="LR726_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216270.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="LR726_001168"
     CDS             16171..17391
                     /locus_tag="LR726_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216271.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="LR726_001169"
     CDS             17381..18967
                     /locus_tag="LR726_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL6216272.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="LR726_001170"
     CDS             19006..21144
                     /locus_tag="LR726_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL6216273.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="LR726_001171"
     CDS             complement(21512..22495)
                     /locus_tag="LR726_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBL6216274.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22873..24264
                     /locus_tag="LR726_001172"
     CDS             22873..24264
                     /locus_tag="LR726_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL6216275.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24280..>24849
                     /locus_tag="LR726_001173"
     CDS             24280..>24849
                     /locus_tag="LR726_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL6216276.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCE"
BASE COUNT         7857 a         4221 c         5077 g         7694 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
    22861 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
    22921 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
    22981 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
    23041 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
    23101 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
    23161 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
    23221 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
    23281 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
    23341 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
    23401 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
    23461 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
    23521 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
    23581 agtattattg aaacatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
    23641 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
    23701 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
    23761 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
    23821 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
    23881 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
    23941 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
    24001 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
    24061 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
    24121 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
    24181 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
    24241 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
    24301 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
    24361 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
    24421 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
    24481 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
    24541 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
    24601 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
    24661 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
    24721 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
    24781 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
    24841 agttgtgag
//
LOCUS       DAFGFZ010000034        24486 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000034 DAFGFZ010000000
VERSION     DAFGFZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.034"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..609
                     /locus_tag="LR726_001174"
     CDS             <1..609
                     /locus_tag="LR726_001174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6216277.1"
                     /translation="LSYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLS
                     KDDKIVMGKRIREVRKNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAY
                     LGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLF
                     SPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            1605..1856
                     /locus_tag="LR726_001175"
     CDS             1605..1856
                     /locus_tag="LR726_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216278.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            1939..2211
                     /locus_tag="LR726_001176"
     CDS             1939..2211
                     /locus_tag="LR726_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216279.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            2301..2540
                     /locus_tag="LR726_001177"
     CDS             2301..2540
                     /locus_tag="LR726_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216280.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            2687..6970
                     /locus_tag="LR726_001178"
     CDS             2687..6970
                     /locus_tag="LR726_001178"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBL6216281.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKVLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            7069..7434
                     /locus_tag="LR726_001179"
     CDS             7069..7434
                     /locus_tag="LR726_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216282.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            7436..7888
                     /locus_tag="LR726_001180"
     CDS             7436..7888
                     /locus_tag="LR726_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216283.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            8502..9812
                     /locus_tag="LR726_001181"
     CDS             8502..9812
                     /locus_tag="LR726_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL6216284.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            9835..10146
                     /locus_tag="LR726_001182"
     CDS             9835..10146
                     /locus_tag="LR726_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216285.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            10507..11763
                     /locus_tag="LR726_001183"
     CDS             10507..11763
                     /locus_tag="LR726_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL6216286.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            11760..12305
                     /locus_tag="LR726_001184"
     CDS             11760..12305
                     /locus_tag="LR726_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216287.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            12306..12806
                     /locus_tag="LR726_001185"
     CDS             12306..12806
                     /locus_tag="LR726_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216288.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            12887..13443
                     /locus_tag="LR726_001186"
                     /pseudo
     CDS             12887..13443
                     /locus_tag="LR726_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13463..13807
                     /locus_tag="LR726_001187"
     CDS             13463..13807
                     /locus_tag="LR726_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216289.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            13826..14737
                     /locus_tag="LR726_001188"
     CDS             13826..14737
                     /locus_tag="LR726_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL6216290.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            14816..15808
                     /locus_tag="LR726_001189"
     CDS             14816..15808
                     /locus_tag="LR726_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL6216291.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            15805..16023
                     /locus_tag="LR726_001190"
     CDS             15805..16023
                     /locus_tag="LR726_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216292.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            16036..16446
                     /locus_tag="LR726_001191"
     CDS             16036..16446
                     /locus_tag="LR726_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216293.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            16498..19026
                     /locus_tag="LR726_001192"
     CDS             16498..19026
                     /locus_tag="LR726_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6216294.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            19042..21252
                     /locus_tag="LR726_001193"
     CDS             19042..21252
                     /locus_tag="LR726_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216295.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            21260..22351
                     /locus_tag="LR726_001194"
     CDS             21260..22351
                     /locus_tag="LR726_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL6216296.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            22372..22914
                     /locus_tag="LR726_001195"
     CDS             22372..22914
                     /locus_tag="LR726_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216297.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            23109..23366
                     /locus_tag="LR726_001196"
     CDS             23109..23366
                     /locus_tag="LR726_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216298.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            23380..23712
                     /locus_tag="LR726_001197"
     CDS             23380..23712
                     /locus_tag="LR726_001197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216299.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            23757..24305
                     /locus_tag="LR726_001198"
     CDS             23757..24305
                     /locus_tag="LR726_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL6216300.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            24312..>24486
                     /locus_tag="LR726_001199"
     CDS             24312..>24486
                     /locus_tag="LR726_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216301.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLI"
BASE COUNT         8425 a         3552 c         5283 g         7226 t
ORIGIN      
        1 ctcagttatg tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat
       61 atttttctat taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata
      121 tttagtttat caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag
      181 aaccgggaag agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat
      241 gttataagta gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt
      301 gcctatcttg gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata
      361 cttccaaaag atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta
      421 aggatgagga aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt
      481 tctccttcaa tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat
      541 actaatcgaa ttatccaata tgcttatata ggaaatcttt ctttagaatt tttgatttac
      601 gggatataaa aatagtttac gtcgatactt attaagttat cgacgttttt tattatagaa
      661 aaaatttata aaaggaggtt tcttgatatg ataagaaaca aaaagggttt gttgaaagtt
      721 gctttactgg cagctttttt ctaagtaact ttttgacaag ctcaatggca acacaagcgg
      781 aagaagtaat acaagaacta actgttatga ataacagcta gataaaatgg gctatgtatg
      841 ttatccaaca atttagtaat cactagaagc ctagcgaaag ttcgcttgat aagttataga
      901 tgagtattga tttgtagtgt ttccaaatca aaagggtaca gagccacatg aaatatgttg
      961 tcctctgcag cgtcaggatg ggaatccata ccatggagat tcggagatct gtgagggaat
     1021 acttacgtgg ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt gggtagattg
     1081 actgaattct tatatgagaa aatgaaacca acaagaagat agggttattt atacagaatt
     1141 ggggatcttt tctggaaaag taatcggata atgcgagatt cgtacggtct tttttaaatt
     1201 tctctaaaaa aaacggcatc gaatgggttg ggtgattgat aagcaatgaa tcatagactg
     1261 atatagaata aaaaatgatt ctatttatct ttttacatct atcgtttttt caagtagccg
     1321 caaattatat cagcattcaa caatttttgt atacaagttc tagtaaaagg atctgaagaa
     1381 aaagttggtt gttgtgaaga taaaaaaaga atcaggtttt actgagtttt tcttcaaaat
     1441 ttgtgttatt aggttaggac aaagataaga agaagtatga agatttaaaa aaggaatgag
     1501 gaaaagatag taatttccga ttattggagt ttttttattt ataaagaaga agcgaataag
     1561 ttgtgattcc tagatttcgc gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag
     1621 tgtttaatac acgaggacaa agtttcttag gtattgaagg cacacgtgta acggttgatg
     1681 ggtggtgtga ttgtttcgag gttggaaaac atgttgaact tatggcattt actgagctaa
     1741 aagataagaa caacgtggaa tttttcgaga gtgatattgg ttgggacgat catgaagaag
     1801 tttatggaaa atttattttg cggaagggaa attcttatac gaatggggaa gcataagtag
     1861 cgatttgtta gagatcattg ataggattga aattgtcggg aatatttatg agaatctaga
     1921 gttatttgag gtgatagaat gagtgatggt tttgaacaag tatcaataat taaaaatcaa
     1981 gttcgggaga ttcttagaaa aaaaggctat ttggtagata gttattttga aggagattac
     2041 gaaacatggg taggtgtcta tgctcgtcca gagaataaac ctacctattt agatcctacg
     2101 acgtcagaag atgcctattt acaaaatagg tatcgagtag atggttttaa gcaagacttt
     2161 gcggagtggt ttgaatggga aattgaaaac ggagaagtaa aagaagaata agagtacttg
     2221 ttgtatcaaa ataagccagt agaacaacca aaccagtagc tagtaatgac aataatactg
     2281 aggaatcgga tcattatggt atgattgata tttctcttcc agaagatctt aagccagatc
     2341 ctgtaacaat tggcaatagt gtcatgattt ttgattcatt agatgaagct atgtcatatg
     2401 gttggaaaga aattaaggat gaaggtagta attggtatgg catgagattt ggtgatggtg
     2461 aagttgccaa aaaagctggt attccaattg ggaaatggac agttgatttc cacgaagcag
     2521 gttctttttt tgttctgtaa ttatagtggg agggtaaaat cattgatgaa atgtgttcag
     2581 ataagagcgc cagataaaaa tagaagagac agttcacgtc gttatcactt ttggtaacgg
     2641 cgttttgttt tattatataa aattctcagg aaaaggagga attcttgtgt atgcaaaaac
     2701 gaaaatgaat tggttgaaag cactcgcatt ggtggctttt tctcaattct gtttcttggt
     2761 gggaactgct caagcacatg cagaagaaac gacggctata caacctacga tggaagccat
     2821 tgcccaagtc gcttctgaag tgacagaagt ccaaccagca gaagccgggg tcccagctgg
     2881 tgaagccact gttgaacagg cccctgtgac agaagcgcca acggtcaatt ccaaaacgac
     2941 tctcgatcag acgattgggg aaacacaaca cccagggaat agtcctggta cttcacaacc
     3001 agttgaagaa actgcctcaa ttacaacccc tgcttcagaa acgtccactc tagaaggaga
     3061 aaaagctgcc gaagcaacgc cttctgtgca gccagagtct tctgagaaac aagcggaaga
     3121 aaagaaacga atagaagaaa cagtggaaca accagcggct actgctgaca aagaaggaaa
     3181 aaatgaggaa aacgttgaaa aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac
     3241 gaaaccaaac aaagaatcta cggctagtat tgaaaatcaa aagaaaaaag agcagaacat
     3301 tccaaaaaca ggtacaccta cactgttagt aacagtacca accaatcgtt cggttcgtgt
     3361 tcgccgtgca accaatgcag ctgttttagg tggtcggaaa attgattggg gcgaaaatat
     3421 tggtgttact tctagtccga acaatctaaa cttcacttta aattatatgg ttcgttttga
     3481 gattaatgga caagtggcgt tttgtattga gcctggtgtc tcagccgcag aaggtcaagt
     3541 ttatacagct actgctttga aaaactattt gaaaaattca aatgtacgga aaaaaacaag
     3601 cttgatctct tatttaggat atgtcacaaa taaagacaaa tccgatgaac aatacattgc
     3661 ggcacaattt atgatttggg aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt
     3721 tgattatgat cgtcgaaaag cagaaattca acgtttgatt aataactttg gtgaaaaagc
     3781 aacttttaat aatcaaacac atacggtaaa aattggcgaa acgttgaaag taaagaataa
     3841 aaatgatttt ttgaaatatg tgaaagacat tcaaaaacca aaaggtgtca atgtatctat
     3901 tcacggcaat gaagtatgga ttacagtcac aaaagaggca ccggataaag caaaaattca
     3961 attgaataaa attaaagttc aaggtgcgcc attagcctat cgtcgtccag gagctcaaac
     4021 aattggtgtg ttacatccaa cagaacctaa tgcgtctgtt attaatttac aaattctaaa
     4081 acaaggaaac attcgtgtgt ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc
     4141 agcttcttta gaaaatgcgg tctatggttt gtatcaagca gatgggaaaa agattaaaga
     4201 aattaccttg aaaaaaatta acggcaaagt acaggcagaa attaaagatc ttgatcctgg
     4261 caaatatgtt ttaaaagaaa tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat
     4321 tgaaattgtt gttgaacctg gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa
     4381 aggaaccatt gatttagtga aagttgctaa taaagattta gtcgattcaa cgaatccaga
     4441 taacaaacca aaattagctg gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt
     4501 ggtgaaaacc gttgtgacag acaatgatgg ttatgctact tttggtaaaa ataccgttgt
     4561 ctttgatacg tacattcttt ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc
     4621 acttgaagtc acaatttctg aaaatgggca aactttccat tatgttttag aagataaagt
     4681 aattgaacaa cgtcttaaag tggtgaaagt ggatcaagaa acaggaaaag tgattccact
     4741 tgctaataca gaattcaaga tttttgatac tttagccaat cgttatgtca caatggagat
     4801 tccaaatgat acagaaacaa cggatatctt taaaaccaat gaaaaaggct ttttcctaac
     4861 taacggtaca cttacttatg gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt
     4921 aaattacgtt ttgaatcaaa acccattagt gttctcgatt accaatgata cagaaccgat
     4981 caaagtgatc cattttgcga accgtcaagc acggaaaagt gtaaaattat tcaagtatga
     5041 agaatggaac aagaagaaaa cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa
     5101 aaatcaagca attggtgaat ataccactga tcaaaacggg gaaattttag tgaaagttct
     5161 agttgccgat gactattact tcttggaaaa tgccccatta gaaagtttcc aaccaaatac
     5221 agataaacaa gcattcacgg ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt
     5281 agtggtttct gtacgtaatt acttgattcc accaaaacta gaaacacaag cgtcaaataa
     5341 agccgatggt gaaaaagtgg ttgatccatt agaaacgatc gtcattaaag acagtgtttc
     5401 ttataccaac ttgtttgtcg gaaaagaata caccatttca ggagtattga tgaacaaggc
     5461 aacgaatgcg ccaattttac aagatggcaa agaaatcaca agtaccttgg cctttattgc
     5521 agacaaagtc aatggcacaa aagaccttga atttgtggtt aatgctagtg ttctgaaagg
     5581 tcaaacgatc gttgtttttg aaaaactttt ccgtgatggc attgaagttg cagcacatgc
     5641 ggatatcaac gatgaagccc aaagtgtgaa agtgaataat ccaaccgttc ataccacggc
     5701 tgtctttgaa gacggattga cagtcgcaga cccaacagga ttgtttacct taaacgatac
     5761 cgttgaaatt accgagattc ttccaggaaa agcatacttg gtcaaaggga tcttaatgga
     5821 taaagcaacc ggtgaaccct tgcttgtgca tggaaatacg gtagaaacga gtgttggctt
     5881 tattgccaaa gcagaaaatg aaacggtgat cgtaccattt acctttgatt tgacaggtat
     5941 aaatggtcgt gaaatcgtcg tgtttgaaag tctttttaga aatgatgaag aactagcaaa
     6001 ccataaagac atcaacgaca aagggcaaac ggtacgagta actaacccaa gtgtacgtac
     6061 aacggctaca cataaaccca ctggcttgaa agaggttaat ccattaacga aagtcaccat
     6121 tacagacgct gtgaaatacc acgatttggt cgttggaaaa acatacacag tcaacggtgt
     6181 tctaatggac aaagtaacgg aaaaaccagt gctagtagat ggaaaagaag tcacaagtac
     6241 cttaacgttt gtggcagaaa caacggatgg tacagtcaat ctagacttta ttctagacgc
     6301 acgtgcgttg cgaggcaaag aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt
     6361 attggcttct cacgcagata ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac
     6421 cattacgacg aaagcgacga ataaaaatgg cggaaaagag atcttggcct tgccaaatca
     6481 aactgtgctt gaatgggtca aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat
     6541 tgttcaaggc tacttaacac cagacggcac accatttgaa ggcactcatg cggaaaaagt
     6601 ctttgcggcc gataaagaaa tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa
     6661 tcttgcagga aaaggcttga gttttgcgga atggctccaa gcgaaaacgg aagatgagaa
     6721 agaaacgttt gaagaagtgg cgaaacacaa tgtagatcta acaaacaaag atcaaacggt
     6781 tcaaattgtg gaagcgccaa aaccaccgca accagtgaaa ccagaacaac gggttaaacc
     6841 agctactgtt gtaccaatgt tgccaaaaac aggtgaacaa acaagtagta tacttatggt
     6901 tcttggatta gtagtattag tcattgcttt aggtataaca ctttataaag tacgtaaagc
     6961 caaagattag tcatgaggaa caagcggtta ggatttttaa ccgcttgttt gattgtaatg
     7021 tagaaatatt tagaatttat tttggttttt aagaggagga aaaactttat ggcagaaaaa
     7081 gcattgcgta tgaagaaaga gtttttaggt caagtatttt tattgggttc acgggaacct
     7141 aatccaccaa aggaaattaa tggtgtgtct gggcgatatg ttcaattaca agcagagtgt
     7201 tcgcgagaag aacagcctgc tttttttgtt tatctcacag ctgaagagga taaaagatta
     7261 cgattaccag aaaaagaggt gcgtatttca tttgaagaag cttattttgt ttttcgtaaa
     7321 gaacgagtga atagtcgtga aacaaatgat atgggacaaa cagagcgtcg aacaactggc
     7381 tatcaagatg ttggtgtctt tttaactggt gtaaaaattg gggaggtttt gtaagatggg
     7441 attaaaattt ccaaataatt atgacattac agaagcatta gatgaaactt gtttaggagc
     7501 cgtacgcttt ggcgcattag aagagtctga tcgtgtattt gataatgaaa caggcgatta
     7561 tacgggagaa attcgatcac agcgagcaca attagttttt ggaaatccag atgaaccaaa
     7621 gattgcacga cgagttaaat ttccagcaga tgtagatttg actggtttta aacgacgtga
     7681 tcctgtgaaa ttaattgatc cagaatatca tgtggtatat attccagcac aagatggcaa
     7741 tcgtgaatat tcagatttag tgatttatgc gaaaggatta gagaaagtag aagctccaac
     7801 aaataatcct aatatgaaac aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt
     7861 tgattctaag gaaaaagaga aaaaataagg tttttttgaa agaataggaa ggagtcttcc
     7921 tattcttttt atctaagata gaaggaaggg gagattttga attcagataa aaccaatcaa
     7981 atactttgag agattgtgct tgttatcaaa atatccaaca attgattgag tattttggag
     8041 acaatccaac gatttttgta caaacaaatg catatatttg tgcggttatt gggcagtgta
     8101 tgtttttacg tgatcttgtt cgtttttcat taccgtacca gtggaaacaa gatcatcagg
     8161 aagtgatttg gattcgtata gtacgaatat gtaatatgtg tgcacaccct tataaaaatc
     8221 gattaaatct ttttcgatta tgttttttta ctaaatgtga tattcctgta ttagttgctt
     8281 tttgtcaaaa gtttttagca caggaaaagt aaaaacagaa aggaggaatg tgacaattga
     8341 aagctcattt tttcaagtat cgtggtcggc gaattcgcta tttcaatcgt tatttatggt
     8401 tttgctatta tggtttcttt ttgtttccat ttttgattct gttaggttgg tttagttgga
     8461 aagtatttta tccacgttta agtacgtttt cgaattggca aatgtatctt attcctggaa
     8521 taagtattgt gttgtatttg ttgctggtta gtgggttaat tttagggttg atgcattggt
     8581 cgcgactaaa agaaggatat tttgcctctg tttattggcg tcagttactt gttcgaatgt
     8641 taattgacaa tcggtttgtt tatacaaaga aacaagttag tgagaaacaa acaagggaaa
     8701 aaatgagatt accaaaagtt tatttttatc aaaagaaaca agaattagtg gtttcttttc
     8761 ctttagatgg tgggcgtttt cacgaacgat ttttaaaaat gggtcatttg ttatctgaag
     8821 tatttttgcg tgatttaatt cgtgaagaac gagaaaaagc ccgaatcaat tatgtattcc
     8881 tttcagattc tggtcggatt cctattgatc aatgtatagc agaaaatgga cgtattcatt
     8941 taatgaaaac tttggattgg gtatatgatc aatcaccaaa tatgttgatt gctggtgcaa
     9001 tcggtggtgg gaaaacctat ttattgtatt ctttaattca agcttgttta agtgttggga
     9061 cagtagatat ttgcgatgga aaagcggcag atttagcagc attaggtgac gtcgatgtat
     9121 tcaaaggaca tgtgttttac aaaataaatg aagaaatgat tatttgttta cgaaatgcat
     9181 taaaagaaat gaatcaacgg tatgcctata tgaaaaccat gaaagatcct aaatatgaac
     9241 cagggaaaaa ttatgcttat tatgggttac caccgcattt tatctttttt gatgaatggg
     9301 cagcttttta tggttcgttg gatatggaag cgagaaatag agtcgataaa ttgattcagc
     9361 cattagtatt aaagggtcgt caagcaggga tgtacattat tttagcaatg caacgaccag
     9421 atgcagaata ttttccaagt ggtgttcggg ataatttaac attccgtatt tctgttggtc
     9481 gattgtctcc cattggttat ttaatgactt ttggagaaga ttttaaaaat aagccatttt
     9541 acaacaaaga tcaacctggt cgtggatatg cttttccagg atttgatgta cctgtagagt
     9601 tttttgcgcc atttgtttac aaagattatc actttatcaa agaatttgag aaatttactg
     9661 aaatgattgt ctatgatttt acagtaaaag aggatagtca agatcaaacg atgggagatg
     9721 ataatcagtt gttacaagaa caactagagc gccaaatgta tttagcaaaa gatccaaaag
     9781 gaacaagttt ttctgatgcg catattttgt aatcaactaa ggaggtgaag ttaaatggca
     9841 gagagttgtt attatagagt agctacagca atacgaatga ttaatccttc tctttcttcc
     9901 agaacgtttt atgattggtt gaatcgtatt gaacaagtaa ctgattaccg ctttttgcgc
     9961 aaagaacgtg tttttacagg aaaagttatt aatcaagtat tgctaacgaa aaaagatata
    10021 gaacgattga ctcgattgta tcattatcga gtagatttag aagaggattt aactttgtca
    10081 atttatcgcg ttttttctcc tgaaaaatat agtgaaataa caaaattaga tcatttaatt
    10141 ttgtagttat tatctatcgg gagggctcta gacgtagtct ggtagccgca ctctgcctgg
    10201 cggtgcgcat taaggctgcg aagcatgggc ctataatgcg cactcgctag cagcgtgtgc
    10261 agctcacaaa gaggtcgtgc gcttgcgcac ggcctctttg tgtaaagccc cccgatatat
    10321 aatatggggg gcagaaataa cataacacat agaaaaaagt tttataaacc cttttgtatc
    10381 aagtgattat ctaaggtcag cagttgtact acttttccca aaaaacgagg gggactagtt
    10441 gatatatcgg tgtttctaaa gggttaaaaa aaatcgattt tgttatttta taaaaggagc
    10501 gagaaaatga caccgattga gctaaaaagc tacagaaaga agttaagtgc aattcataat
    10561 ataagtggag ataaaaagcc gctatcacaa gcaagatttg cgcaattgat gggctgctca
    10621 aaagattaca ttagaaagat ggaacaaggg aaaagaaaaa tcccagagga ttttgaacaa
    10681 cgaatcaagg aacgctttcg attgtatatg aaaagcggaa tagatcttcg agcaatgatt
    10741 gattatttgc gtctttcctt ttttgggaca gactacaaaa cagtgattca acgtgtatta
    10801 cgaatggatc tgacagaatt tgaaacacgt ccaacgggct tgtatctttt tgactgtctt
    10861 tctgttcgtg ggaatatctg ggtgttctgg cattcaaaac gaactgtaac agaaaatgta
    10921 cttgtgcaat tttcaggaca aggttgccgg gaatatgagg aagtcatgcg tgaacaacaa
    10981 acagattggc gaacatggct atattccatt tggaaaaatc aaggaaagtt ggaagctggt
    11041 tatacacgtg tgcaatgtag ccgattcgat attgcgttag atgaactttg gggattggaa
    11101 acggaagaac atttcgatct ctttgaatta ctagaaaaac aaaaagcagg tttattagaa
    11161 atggacttta agagattcca gaatattggc ggttgttttt tagatgatgg ctattttcga
    11221 agtgaaggat tatctttgta ttttggatcg cgaaagagtc ctctcttttt taatttttat
    11281 gagaaacgtt ttgaaattgc gaaccgagaa aaaatttcag aattagaagc attaacgaaa
    11341 tatggtattt ataatcgata tgagttacgg ttagccaatg agaaagcaac acaagcagta
    11401 gaagcattta ttttaggtga ttcaccgaaa cgtttaggag agattggcgt tggtttaatt
    11461 aatgcaaaac ttattgtttg ggatagactg gaaagtgatg cacggattgt tgataaacga
    11521 tgggcaaaat tatttcgttc gatggaagaa ttggatttta cagtaaagcc agaaaaattc
    11581 tcattagatc gttcagaacg ctggtttttg aaacaagtag ctccgacatt aaagttgtta
    11641 caagaagcag atcgtatttt aggaagagac tttgtacaag aagggattga taatgccgaa
    11701 ctaaatgacg agcaacaaaa ctatctagaa tggatgggaa cgtcaggagg gaaaattgca
    11761 tgaatgcata tacgacaaca gaatggctgc gcctgttgga tttaaaggct gcaatcgaag
    11821 cattaaatga aaagatggtt gatttgtctt attttcgttt tcgagttcct tatattgaac
    11881 aagcggtaaa agctggacgt tatcaggaaa aagagaactg gcaagaaatt gcacgcttat
    11941 tagaagttcg taagggatac gaacaagaac tcgaagagtt ggaatttagc cgaaggaaag
    12001 gaaggctaga attcattcgc ttttatcgat tttcattgcc aatacctgcg attttagcgg
    12061 ttaaaaaagg ctgcgataaa atgaagatat atgaaaactg tgtggcagca cttagcagcg
    12121 aaaaacctct tatggaagaa atttcattgg tgactgtcac ttgggaaatg agtcgccagc
    12181 caacagaaga acagacatat ctttctttag aagagattga gatagaatta gaggaaattg
    12241 gtcgctatgc cacttgttct acctattgtg gatcggtcat tagtattgct ggtgtgattg
    12301 tctaaatgaa aaaaattatg atttatgttg gtgcgtattg gtcacgtgat ccaacagtat
    12361 tagaaaatcc agaggcggta cactattgct tgcgccaatt attctatctg tataaagaac
    12421 gattagaatc gttgattcgt cagttaccat atactgacag acgtttagat gaacttcttc
    12481 taaggtatcc agcaatgtat aaacgacgaa aaaatagatt gttaccagaa gagtatccca
    12541 ttgaaaaaca tgaacttgaa gggcgatttg tggcttattt ctatgatgat gttcgtatgc
    12601 gcttagtaga acaacgaatg gaaatagaaa atgatcgcta ctattttatc aattattgta
    12661 agaagcgtaa gtatcaagta actgatgatt tttatgattg tattttacaa gatggggaag
    12721 tgattctatc caaaattgct ccaagttttc gtgaacttgt gccgattgat tttttgaaaa
    12781 aatgtcattt gcggattctt ccgtaaaaaa gagctataaa aggaaagaaa agtagaagcc
    12841 aaatcggttt ctgctttttt atttttttta ggaaggagaa gatggtatgt cactggaatt
    12901 tttaggagag ccaattgaaa aggaagagtt cgtttcgcat tatatggatt tgtttattca
    12961 ggtgataaaa aactttgtgc gattgatgaa ttttatccac gcgaacaaga ctatctagcc
    13021 gctgaacgaa taaaaatcag tttattatgg gaaaaattca cagatgaaaa taaccgaccg
    13081 ccagattatc gctatttatc agattgttta acgaataatg tgctttctaa agaataccta
    13141 tttcacaaac gtgagaaaaa tcaaatgtcg gttgaacatt tttgtgattg ttatattgca
    13201 ttattgcgta gtcaacagtt attaacagta gaacgatatg gtgcagagtt tggttatgtc
    13261 atgcaagctg agcgtcatag agctacaaga tgctatgaaa aagaagatca agtgattgat
    13321 ggctatgaag aattatgtat tcgaaacaat cgctatttgc aacaacgttt atatgaacaa
    13381 ttgatgatgg atttttcaaa tctctataga gggtatgaat gttcgatgat tcaagaaagg
    13441 tagaagaaaa gggggaattt ttatgtggcg attattattt ttggtggtat gtggtatggc
    13501 attgttctat gtgtatcgtg tctatctcta tccacgaatg gaacgatgga caagttggta
    13561 tagagatcct ttaaaacaac gaaaaggatt gaagcggtat cgttatgaat tacttgttga
    13621 attttcggat gggcgacaag cacagtatta tttttttgca aactatccaa aatacaacac
    13681 cagtgaacta ttgattcaat tattcaaaaa taatccatat gttgggttag aagaaaacgg
    13741 acgggagttc ttttatcgtt cagataatat tagtgcggcg tctatgaaca aaacacgaat
    13801 tgcataaaaa attggaggaa taatcatgaa aaaaacaaca ttagcagtcg taacaacact
    13861 aggaatttca ttattttcgt ttcaattagg gagttcattg gtttatgcag atgaaactgt
    13921 agaaacacct aagtcagaaa ttgtagaaac attgccaggg gaaggattac cagatgttga
    13981 gacagagcta aatggaggaa caacggaaaa accagcagaa cctgaacaat tgcagtcaga
    14041 gaaacctgaa aaagagccag tgacaccaga accagagttt cctgaatcaa ctttaccgac
    14101 tgattcgatc gaagaagaag gtgtgcatag ttcaacaaca acggattcaa gtatagtcac
    14161 aactccggaa caatcaggaa caaccagttc ttctgaaaag aatactgatt cgtcaagtac
    14221 ggaacagcca acgatgtcgt ctaccagtga agaacaaaaa gaagaaccga aacaacctgt
    14281 tgaaaaatca gaagcaattc caaaagaaaa taaacctatt gttccagcaa aagaagtcac
    14341 agtatcggta accccaagcg gtgaaattac gacgaatact tctcaaggaa tgagtgtacc
    14401 gattgtcact agtaatgtag aggaattgac ccatattcca acaccaacca ccccattaaa
    14461 agtagcaggt gatcaaacaa ttgttggtgt aaaagatggt attccattaa tccaagatag
    14521 tgaagggaat ttggtaaagg atttgtcgat tcctgtaaag aaacttccaa gtggcaatat
    14581 tgaaatcaaa acagcagatg gaaaaacaaa agtcttacca aagactggtg aagaaataca
    14641 tgttgcactt tctgttctag gaggagtgtt gacaagtatt gctggatttg ttggatataa
    14701 aagaaagaga aaaggaatag aaaatgaggg taattgaatg gactattttt cagaaaaaca
    14761 agcttatcgt tgtcaatcgt acgtactagt aaaaacatac tagaaggagt agcttatgaa
    14821 aaaacgacaa ctacggcgga aagaaaaagt ttatacagag aaagtaccga agcaaaagaa
    14881 aatccattca aaaaaagcag tgaaatggat ttggtgtttg gttttatttc tttgtttttc
    14941 agggggcttg gcattttttc aatccatgaa aacgaaggtt caagtgtcgt ttttgaaaga
    15001 tgaaatccaa caattacaaa agaaagaagt acaaagtcgg tcagaattaa cgtacacacc
    15061 tgaaattgaa tcctttatga atcgttttgt tgctttgtat atgaatacat tcgataatca
    15121 tgaacaagaa caacaacgtc aagaaacatt aaaaaaagag ttttatgcac aaaactttat
    15181 tcaagaacca ggaagtgttt caggtgttcg aaaattaact gctgcttcgt ttattggatt
    15241 aaaagaaatt gaaaccattc caactgctag ttataaagta acttatgata ttcgctcgat
    15301 gaatccagag agtaagaaag aaacaagtgt gacaatcaca caagtactca atcttccctt
    15361 ttctatttca aacaagggct gtcgtattat tgcttatcct tattttacgg cactaccaaa
    15421 gaatacagaa aaacaacgaa tgattactta tgatgacaca ttatacgaaa cggttgatgc
    15481 gtcggaagcg aaagatgttg aaggatttgt ggaagatttc ttagataagt atgcgaaaaa
    15541 caatgcaaaa gatatgtcat acatgatgaa agaacctgaa ggactcaatc aaacagctaa
    15601 agttgatcaa gtagagatga aagtattgcg ttataaagaa acgaaaaaac cttttattgt
    15661 aaaagcaacg gtcgtctttc gagatggtga tacgtcactt ttacaaaaag aacaaatgac
    15721 gattgtcatt acgaaaaaaa caaatcagta ttatgttgaa aaattaacac ataattggtt
    15781 ggataggaaa ggagaagaac ggaaatgaca ctgaagggaa ttgtagattt tatattaacg
    15841 caaggtggct attttttaat aatggtggtt gtttatctgg gattgcgaaa ttttatgaaa
    15901 tcaaaagtag gaaatgcatt aatatctatc gcagcaggtg ccgcagttta tttcttctta
    15961 aatgatccaa cacgtgtatt tgatgcgttt ggtaaaatca ttgaaaaatt atttatggat
    16021 taggaggata gtgaagtgga ttgggaacct tatgattatt cgattgttta tgcggaacct
    16081 tatcgcgtac aacagattac aagcaattgg cgtcttccat ttccagttgc attagtcaat
    16141 gcagcaactt ttttgttggt cgtaatcttt gaatatttat ttttacgttc atttattggt
    16201 tgggtcattt ctttttttcc accagcagga gttgtttttt atataggact cccctattta
    16261 tgtgtgttag cactggcaaa actaaaacct gatggaaaaa aagtgcattt ttatttatgg
    16321 gattggtttt gttatatgat tcgtatgatg aatggtgatt gtgtgtatta taactgggac
    16381 aaggtatcaa acaatgcatt ggaagaatta ctttttgtga ataagtatgt ttatttagag
    16441 gattagaaaa agggacaatc aactggttgt tcctttttta ttgggaggtg aaattcattg
    16501 gtgcttgtaa aagaaccagt aaaagcattc aagaaaaatt gtttgttaaa tggagaggat
    16561 tgtatctggg cctactatca tgtttttcca cgaacaattt caaaaaacac accaaaaaag
    16621 acacaggaaa taaaagaaat gatggctcgt ttactgttgc ttttagcaga ttatgaagat
    16681 gtggagttgg tgatgtatcc acgaacaatg aatttacgga cgaagtttga cacattagga
    16741 aagggatttt ctcccgatag tcaagcaatt ggtgcgtatt atgcaaaaga aacagtgaca
    16801 ttgttggaac gagaaggtca agtctatcgt ccttcattta ttttaggaat caaagtcaaa
    16861 gaaaaagcaa cggtggagag ttttcatgat gtaataaatc taatctggtt agatttagca
    16921 ggaagtttag cacgcttatt tggattagaa gtagatgatt tggaaacttt gttttctgaa
    16981 agtgatcctt atgtaaaaga agtgacacaa ttactttata gcttttctat tcgtccctta
    17041 gtaaaaaaag agctgttgta tctcaatcgt ctgcaatata ttcgaggaat ggctcacaaa
    17101 gtagaagaac aaaataattg ggaagtactg aaagacaatc aaggaacgta tgtacgtata
    17161 ccgaaggaac ataaaggcct tcttcaatta gaagatgagt tagggagtag ttatttagca
    17221 ttgttgccta ttggggaaac acctgaaaat atgagaaatt gtgatctatt tcaatttgct
    17281 caggatcttt ctttcccagc agaattgcga atgaaaattc actatccacg cttgaaagga
    17341 cagggaggta ttggtttaaa actgaattgg ctatcggaac ggttcaaaac agagcataag
    17401 gaattggttc aaaatgatga agaacctagt gaacgcttat taactgttcg aggattagta
    17461 aaacatttaa agcaacgagt agataatggg caacctgtcg tcgattggat cgcatgtttt
    17521 attgtatacg gtcaaacggt tgaagaagtt cgtcaaaata gtgcaacggt tattcaacaa
    17581 cttcatacga ttatccatat ccaccgtgca aaaaatgatc aacgagaatt gttctataaa
    17641 tgtttattag ggcaaccatt gggacggcag ttacattgga agcaccgaac aactgtagaa
    17701 gcagttggcg aatggttgtt ttcagcagta tcagaattag gtatggaatc cggctggtat
    17761 attggtcggc aagatacatt aggggatggt ggttctcgag gagaaaaaac accattagaa
    17821 gaattaattt atgcgtcaaa tcgttttgtc tttttgaatc ttctagccat tgcggaagga
    17881 atcaaaggtg cgttgtttga tgcgcctcat gtagcagtaa ctggaaaaac cggtaaaggg
    17941 aagacctttt tagttggctt gctctttttg tatagttcgt ttatgaatgt acaaagttta
    18001 tttgtggatc ccaaaggaga aaagcgcaaa tggtttacga aactcattca agacccgtat
    18061 tatcagaaac attacccttt gtttgtagaa catttgcaaa aaatgagtta tgtcacgtta
    18121 gatgctacaa atccccgtaa ttatggtgta ttagatccaa ttgtttgttt atcccgagta
    18181 gatgcgaaac aagtagccca ggatatgatt gatgagttaa gtccgttagg taccaatcac
    18241 cttttaaaag gggcggtatt aaagggaatc caagaagttt tagttcaaaa agaagccgga
    18301 caacaagttg gcttgatgca tgtggtcgac tatttagaac aaatggaaca aaaagaggtt
    18361 cgagaatatg gtgagttttt acgcttgacg gtagaagatt cgattttacg tttaggattt
    18421 tcttatggag aaaacccggg attagacttt aatgccaaaa caacgatttt agagatccaa
    18481 gatttgaaat taccaaatga caatgtccga ccagaactat atacagatgc ggatcgaaag
    18541 tctttgtgtt tgatgattag tttaggtcgt ttttgtgaaa tgtttggaaa acgagatagt
    18601 agtaagaaaa cagcgattta ttttacagaa gcatgggtat ttaataattc gaacgcagga
    18661 cgtagtatta ttgcagcaat ggcacgtgtg gggcgttccc agatgaatca attagtgtta
    18721 gatacacaat ttattggtga cttaggttca gataaagaaa aagggaattt tggtgtcgtc
    18781 tttgcttttg atgaagacag tgagcgagaa aagattttga atcatttacg tttggaagta
    18841 aatgaagcga acttagctga aatgagccac atgattaaag gacagtgttg gatgttagat
    18901 ccctatggac gtgtaggaaa actcaatgta cacagtttgt ttgaagaaat gaccgctgca
    18961 ttacaaacaa cggaaaaaac aagtcactct caagtagaag aacaggatac gttaatcatg
    19021 caatagaaat gaggtgaaac aatggcttat ataaagcata aggaaaagaa atggtgcgtt
    19081 ttgttttttt taacgattgg attcggactg ctttttgttg gtggattggt gtatgcggca
    19141 gaaaagcagg tggatcaatc gatctatgag attgggaaat ttcatattga taccttttct
    19201 tcttatttat ctgatagtga attttcatta ggcaaaatgt tatttggggc aaatggcaca
    19261 gattttctaa aaatgatgac gaatatgttc tttagtttaa gtaaattgat ttggcaaggc
    19321 cttgattttg tcattgagaa gctctatgaa ggagctgcaa tgaacgagtt aattcaccag
    19381 tttttcactt tttcggatac tatttattca aaaatatacg atcctattgg tgcggggatt
    19441 gtcgtcttgt atgtgttata tctatttact cttcgtatat ttagagggaa acattacgca
    19501 aaaatggtgt ttattcgatt tctcttcgtt gtcatttttt caatggtttg gtttggctat
    19561 ggtagtaacg tacctagtaa aggagaagct tttaccaaag ggttaaatga tttgtccgtg
    19621 gaggtagaag gctttatttt ttctgctaca aatagtctag atgaattggc gacaaaagat
    19681 gtcaatggtg ttttagtgcc aacgacacaa aaggaagcca ttcaaaaagt gcgccaactt
    19741 tattataaag cggctgttgt tgatccgtat atattgttaa attatgggac aacaaattta
    19801 gaagaattgt atcaagcaga gattgaacca caagaatttc tagccaagga agcgtcaagt
    19861 gaagaattat ctgatattga gacaaaagtg accactgctg caggagaaga tagagggaaa
    19921 aagaaggctt atcgtgtcta tattcaacca actaaagctg tttataaagc agtcgttggt
    19981 tctatggtgc cattgcttaa tctaagtatt ggattaccac ttgtagtgat tggcatgatt
    20041 cgttttatct ttcaaatgtt ggtgttgtgt ttattacttg tgattccttt ttcattactc
    20101 gcttcttttg ttccagtatt tgattatatg ctcatgaata ccttaaagtc ctttttgggc
    20161 ttgatgtttc aaaaatcttt ttacagtgtg attattttgg tggcattttt gatctataac
    20221 atagtagacg atttaattcc aatgaatact gtttttggtt ttttaggaaa tatgtttgtc
    20281 aaagcactcc tatcaatttt actcattgta aaacggaaag atttattaaa gaaactaggg
    20341 ttaggagctg cagatcaaac tataaacaac acgaaaaaca tagcaagtca ttctttgtct
    20401 caaatgaaac aactgcctgg tcaactatca actgctggac aaaatttggc aatcaaaggt
    20461 gcaggattag cagggaatgt gtatcgagca acgaatccac tcgcttctgc agcgatacat
    20521 ggatttcaaa agtttcaaca aaaacgtaca ccacaagaat cggtaaaagg gaggcaagtg
    20581 gttaatccct ttgaactagg tgggcgttca aatcaagcct tgccttatag tggtcgtcat
    20641 gttcgttatc atggggtaga gcctgaaatg tcttctacca aatcgaaaac agagtggaat
    20701 tctggtcttt caaatggttt gattccaaat tcgtccatga attcagtaag accaaaacaa
    20761 caggtacaat cattggcaca attgagtaag caacaaggga ataatcaaag aaaaccattg
    20821 ttaaataaag gtatcgcttt tgttggtcaa tcgattccgt tgccttctgg aaaagtaccc
    20881 gtgccacaat taacagctgc tggttctctt tcacaacaga ttcgccaagt gaaaaatcag
    20941 gcgaaaaatg ggagttatcc agcaatgcag cagttattga accaatactc tgttcctaat
    21001 tcgtcggtag ccactctatc aggaaatcgt tctgtgcaac aaccggggca acgttcgtct
    21061 gaaagtaaac cgtcgatttc cgcaccaaaa tcgttacaac ctgcattttc ttctttagat
    21121 aagccaacag agcaatcgga aaaaatagaa aaggatccta aaacactgcc gattttggac
    21181 gcaaaaccag gaactgattt tactttacca aaaggacgaa cagtccaaaa accattatcg
    21241 aagaaaggat gaggaagaaa tgaagaaagg gtgtctaggt tgtcttcttt tctttattct
    21301 tccctttttt ctattgccat tctttttttc aggagaactt tctgagcatg gaggagaagc
    21361 tacaggtcta gctgtcgctt ttaatgggga atataccgaa ggattaccga ttttttcaga
    21421 gattaaagga cgtggtccaa tcacagatga acatgcgcaa gcagcagttg gtgcagctgt
    21481 aaagtatcag ttattaccga gtgtgattct ttctcaatta ggctatgaat cagcttatgg
    21541 acagtcacaa tctggaagaa acgatacgaa cttttttggg attacctggt ttcctggttg
    21601 tccctaccca caaggttctg cacgtggaat tggtggttct gaaggtggtt tctatatgaa
    21661 gtttccgaac ttaaaagctt gttatgacta ttatggcttt atggtggcga gccaaagcaa
    21721 ctttaatcaa tgtgtgggca ataaagatcc tggaagttgt ttattgattc taggtcgtgg
    21781 aggctatgcg gcagcaggta ttaccgaagg gagtccgtat tatcgaggag caatgagtat
    21841 cataacgtcc tataacttaa cggaatatga tacttttgcg atcaagcatt ggaatagcct
    21901 accaacaaaa aaaacttcgc ttattgcagg aaaaagtaaa ggcaatgcga aggttttgga
    21961 tcggatcatt ggacgatccg taaataatgg ggaatgttat ggtggtacgg catggtatgt
    22021 atctcaatta ggtggcccac agttaatggg ttctggacac atgtttgcga tgttaatagg
    22081 ggaagattat aactggacaa cctatggttg gcaagtaatc atgaacccaa aaccaagtga
    22141 tttacaagca ggagatgtgg tgaattggca agcaggtggt gcattgtctc ctggaatcta
    22201 tggtcacacc ggaattataa cagatgtttc atatggcgga caacatttct ctagtgtgga
    22261 acagaatgcc gagcaaggac ggattatggc tcggtatcaa cgcacgtaca atcaaacaaa
    22321 aattcgtagt ctggttcgta aggtgaaata agaaaggcag tgactagaaa cgtgaaaaaa
    22381 gggataattt tagtatttag ttggttatgt ttgataggtc ttactggttg tcattggttt
    22441 tcttctgaaa aacaaaaaac acaattgaga acgacagaag aaacaatcga agcagtaaaa
    22501 gaaacaagtg gaactcttca aagtactgaa agtaacagtc aaattggttc attagaagac
    22561 gagacagcgc aacaaatgca aatagatgaa atgacaaaaa atttcttaaa agattttgct
    22621 agaaaatgga gtaatttcac agatgtttat aaacgcaatc aagctgtgag ggactatatg
    22681 acagatcgtt gtattaaaga caatagtatt gatgtagacc cgcatgtaca aatgaaaaca
    22741 actgggaaac tttaccaggt gacacaagat atagagaatc cggatcaata tttgttattt
    22801 ggagaagaaa caatgaataa ggttactcaa tttgtattac ttcaattgaa agtagatcaa
    22861 gaagctcaaa agattgatgc gatcaagatt tattatgtac ggagtgcgta ttaaaataaa
    22921 aaatgaacac tcatatttat tatacaccga agaaatagaa aaaaacagac tgttttttta
    22981 agaaaaacta tgtcattctt tacttgaaaa aagttgttat aggacagaaa ggataaaaca
    23041 aaaacggaaa aaatatgtct tatgaataac tataagaact attctatttt ttaggagatg
    23101 gatacgagat gaaaaaacta attggctata gtgtgatgtg tattggattt gttggtgtgg
    23161 gattgtttgt atcgactgaa tctgcttctg cggctgaatg gtataatcct atctggtata
    23221 atccgaacat tgaatgtcaa aaaggctatc cgtatggacc gacaaatctg aaaaatggaa
    23281 aatgtagagt aaattggaat ggcgttgtag atagtgtaat tactggttca atggactctg
    23341 ctctacatgg ctttacgggt cactagaagg aggaagaaaa tgtctgaaga gaaaaaagaa
    23401 atcgttgaat cattagtagc tttaaaagat tctctttcta agattgaata tgttgatagg
    23461 caagaaattc aaaaactaat tgatgatact attattgaaa tccaagatgc gcgttgtgaa
    23521 ggtataaaaa tttctgtagc tttatctaaa gtaatagaaa aaatgaaccg ttcattagcg
    23581 tttaatggat tgaagctaga tagacaaact agtctgatat gggatcattt aaaagacttg
    23641 tatgacaagt ctaaaagaag tgaacggact gctgtatcaa tcttaaaagg cctttgggga
    23701 atgaattcat aaacaagata tttaaaaagt aacttatttt ttatggagga gaaattatga
    23761 aaagaataat taaaatattt ggtgttttag gtgttttctt attaggttta agtacttttg
    23821 ttagtactgg aacaagtgtg tatgccacag aaaaagtgaa tacagttgat ttagaaataa
    23881 agaataaatt agtagatgaa ttaacttttt attttgatac agttggtttt ttagacgaac
    23941 atggtgtata ccatataaag gattttggtg ctattcgtga acgaatcagt caagataacg
    24001 aacaaggaag agcagcaaga gtcttttatg aacatattat tgttcctaga atggagaaag
    24061 ttgattggaa acgatatgct ggatgtgtag ccattaattc tcttcctgga ggaggagtgg
    24121 cttgggctat aggtacgcag ggagatttta ttgaagcatt aaaagctttt aattttgaaa
    24181 aagctgcgga tttattatta aaaattgcag aaaaacaatt aggaaaagct gcagctaaag
    24241 agttagctaa ggtaaatatt gtagcaggaa ttgcagcagc tgctgctagc tgtgcattag
    24301 agtaaaagtt tatgaaaagt ttagaacaag agcatatggc ttttaaacaa gcaatgttta
    24361 aagaaaatat ctatttaaac cataactata ttcgtgtttc taaggcatgt tcaccagtat
    24421 tgaatatgct tggaggtgga aatggattat atcatttact gtttgtcgat gtttgttggt
    24481 taattt
//
LOCUS       DAFGFZ010000035        24239 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000035 DAFGFZ010000000
VERSION     DAFGFZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.035"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            33..719
                     /gene="gpmA"
                     /locus_tag="LR726_001200"
     CDS             33..719
                     /gene="gpmA"
                     /locus_tag="LR726_001200"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL6216302.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(1017..1724)
                     /gene="deoD"
                     /locus_tag="LR726_001201"
     CDS             complement(1017..1724)
                     /gene="deoD"
                     /locus_tag="LR726_001201"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL6216303.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(1741..2559)
                     /locus_tag="LR726_001202"
     CDS             complement(1741..2559)
                     /locus_tag="LR726_001202"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL6216304.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(2583..3749)
                     /gene="deoB"
                     /locus_tag="LR726_001203"
     CDS             complement(2583..3749)
                     /gene="deoB"
                     /locus_tag="LR726_001203"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL6216305.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3848..4804)
                     /locus_tag="LR726_001204"
     CDS             complement(3848..4804)
                     /locus_tag="LR726_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216306.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(4804..5937)
                     /locus_tag="LR726_001205"
     CDS             complement(4804..5937)
                     /locus_tag="LR726_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216307.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5930..7492)
                     /locus_tag="LR726_001206"
     CDS             complement(5930..7492)
                     /locus_tag="LR726_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216308.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(7704..8786)
                     /locus_tag="LR726_001207"
     CDS             complement(7704..8786)
                     /locus_tag="LR726_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6216309.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8852..9250)
                     /locus_tag="LR726_001208"
     CDS             complement(8852..9250)
                     /locus_tag="LR726_001208"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL6216310.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(9271..9933)
                     /gene="deoC"
                     /locus_tag="LR726_001209"
     CDS             complement(9271..9933)
                     /gene="deoC"
                     /locus_tag="LR726_001209"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL6216311.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9979..11280)
                     /locus_tag="LR726_001210"
     CDS             complement(9979..11280)
                     /locus_tag="LR726_001210"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL6216312.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(11421..12437)
                     /locus_tag="LR726_001211"
     CDS             complement(11421..12437)
                     /locus_tag="LR726_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL6216313.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(12745..13350)
                     /locus_tag="LR726_001212"
     CDS             complement(12745..13350)
                     /locus_tag="LR726_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL6216314.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            13546..14469
                     /locus_tag="LR726_001213"
     CDS             13546..14469
                     /locus_tag="LR726_001213"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL6216315.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            14528..16093
                     /gene="guaA"
                     /locus_tag="LR726_001214"
     CDS             14528..16093
                     /gene="guaA"
                     /locus_tag="LR726_001214"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL6216316.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(16584..18215)
                     /locus_tag="LR726_001215"
     CDS             complement(16584..18215)
                     /locus_tag="LR726_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL6216317.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(18229..19902)
                     /locus_tag="LR726_001216"
     CDS             complement(18229..19902)
                     /locus_tag="LR726_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL6216318.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19915..20232)
                     /locus_tag="LR726_001217"
     CDS             complement(19915..20232)
                     /locus_tag="LR726_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL6216319.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(20257..21657)
                     /gene="lacG"
                     /locus_tag="LR726_001218"
     CDS             complement(20257..21657)
                     /gene="lacG"
                     /locus_tag="LR726_001218"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL6216320.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21894..22679
                     /locus_tag="LR726_001219"
     CDS             21894..22679
                     /locus_tag="LR726_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL6216321.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            22682..23374
                     /locus_tag="LR726_001220"
     CDS             22682..23374
                     /locus_tag="LR726_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL6216322.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(23648..24019)
                     /locus_tag="LR726_001221"
     CDS             complement(23648..24019)
                     /locus_tag="LR726_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL6216323.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         7035 a         5394 c         4376 g         7434 t
ORIGIN      
        1 aatataaacc tatcttaaaa ggagagctaa acatgccaaa attagttttt tctcgtcacg
       61 gtcttagtga atggaatgca ttgaaccaat ttactggttg ggctgatgta aatctagcac
      121 cagaaggaat cgaagaagca aaagaaggcg gacgcaaaat caaagaagct ggaatcgaat
      181 ttgatgttgc ttacacttct gttttaactc gtgcaatcaa aacatgtaac ttgatcttag
      241 aatattcaga tcaactttgg gtaccacaaa tcaaatcatg gcgcttaaac gaacgtcact
      301 atggtaaatt acaaggtttg aacaaaaaag aaacagctga aaaatacggc gacgaacaag
      361 tgcacatctg gcgtcgttct tacgatactc ttcctccatt aatggaagaa actgacgaag
      421 gttctgcagc aaatgaccgt cgttacgcaa tgttagacaa acgcgatatt cctggtggag
      481 aaaacttgaa agtgacatta gaacgcgctt tgccattctg gcaagatgaa attgctccag
      541 cattactaga caacaaaact gttttagtag ctgcacatgg taactcatta cgcgccctag
      601 ctaaacatat cgaaggtatc tctgacgaag acatcatgga tcttgaaatt ccaacaggtc
      661 aaccacttgt ttatgaatta aatgatgact taacagttgc taaaaaatac tacctataaa
      721 aaacactata aaaaagggac tgtgacacga tttgtcacag tccctttttt cgtataaacg
      781 gttttctcct gccaacttct cggtcacaag tcacaatatt catcaaacat gaaaagctgt
      841 tttttgaaat tgttccttgt gactcagagt taaacggcag attcacaacc tcttggtaaa
      901 cggtgttcaa aagcttacat ttgcaataat aagtccagcc aagtcaaaaa catgaggagc
      961 tatttttgct tggatgttct tattacttaa tgcagggcag ctttttcatt tccttcttat
     1021 tctgctatcg ctgtttccaa tcctacttcg atcatttcat tgaacgtaga ttgacgttct
     1081 gcagctgttg tttcttcacc ggtgatcaaa tggtcgctaa cggtcatcaa acttaatgtc
     1141 tgcacatggt atttcgcgcc taagtaataa agagccgctg cttccatttc cactcctaaa
     1201 acgccaagtt cagctaattg gaacgtctct gtcaaatcat ctttgtagaa agaatcttcc
     1261 gaaagaacat ttcctacatg aaccgtatag cccttttctt tagctatctc atatgctttc
     1321 agtaatagat tgaaatcact gattggagga aaatgaaagg cttggaaatt tttttcgatc
     1381 atcgaagaac tggtcgctgc accttgtgct agtactaaat cacgaacatg gacgtctttt
     1441 gacaatgcac cgcatgtacc tactcgaatc aatttcttta caccatacga ctggatcaat
     1501 tcatgggcat agatggtggc tgatggcatt cccattcctg ttccttgaac agaaatacgt
     1561 tctcctttat atttgccggt aaagcctaac ataccgcgta cctgattgta acaaactgga
     1621 tcttccaaaa aagtctccgc aatatattta gctcgcaaag gatctcccgg aagtaaaatc
     1681 ttatcggcga tctcgccttc tttcgcttca atatgcacac tcattttatt gttcctccta
     1741 ttacaataat gtcaatgttt ctttaaccaa tgctttaaaa gattgtttga ctctctgagt
     1801 tgtttctaca acttcttcat ggctcagatt cgcttgcata cctgctgcta agttggtaat
     1861 acaagagatt cctaacactt ttaatccgct gtgagctgct acgatgactt ctggaacagt
     1921 cgacatccct actgcatcgg ctcctattgt ccgagacatt cggatctccg caggcgtttc
     1981 ataagttggt ccagaatagc ccatatatac gccttcttta agatccaaac ctagtttttc
     2041 tgctgctgct tttgctgttg cttgatagtc aggcgtataa gcttgactca tatctggaaa
     2101 tctagggccc attgcatctt cattcggacc gatcaaagga ttttcaccag taaaattgat
     2161 atgatcggta atcagcatca aatctcctgg tacaaatgat tcgttgacac caccacatgc
     2221 attagtcaca atgactgaat gggcccctag tccggccatc acacgtactg gataggtcac
     2281 tgtttgcatg gaatgacctt catagtagtg gaatctccct tgcatggcta acactttctt
     2341 gcctgccaga gacccataaa ctagctttcc tgcatgccca actactgtcg aaacaggaaa
     2401 gaacgggatc tcatcataag aaatcattac agcatcttcg atttcatctg ctaattcacc
     2461 taatcctgaa cctagaatca aaccaaaatc aatttctttt actcctttat caaggataaa
     2521 ctgggtcgtt tcttgtaaca tctcgcttaa cttggtcatt tgctttctcc tatttaaaaa
     2581 gattatttta ataatttcaa gaagctttcg ccattttcag tcgaagaaac actaaagttc
     2641 tcagcaatcg ttgctgaaat atctgaataa tatccttgag gcaatgcccc ttgttctttc
     2701 atttttttac tgtaaaccaa taacggtaca tattctcgcg tatgatcggt accagtaaac
     2761 gtcggatcgt ttccgtgatc ggctgtaatc attagcaagt cgtcttcttc catcgcttca
     2821 agcaattctg gaattcgtaa gtcaaattct tcaatcgcat gcgcatagcc gactacatcc
     2881 cgacgatgac cgtataatgc atcaaaatct actaaattgg taaagctgat tcctgaaaaa
     2941 tcgtttttca taactttcag cagctggtca acgccatcca tattgctttt cgtgcggaca
     3001 gattcagtga ttccttgtcc gttgaaaata tcgttgatct ttccaacagc aatcacgtcc
     3061 tttcctgctt ctttcaaaga atcaaggact gtgtgtccga atggattcaa cgcataatca
     3121 tggcgattgc ttgttcgtgt gaagtctcct ggttcaccca cgtacggacg tgcaatgatt
     3181 cggccaatca tatacggatc atcttttgtt atatcacgaa cgaattggca aatccgatat
     3241 aattcttcta aaggaatgat ttcttcatgt gccgcgatct gaaggacagg atcggcagaa
     3301 gtatatacaa ttaaatcgcc tgttttcatt tggtgttcac cgtaatcgtc aataacagct
     3361 gttccgctat aaggtttgtt acatacgact ttacggccag aaaattcttc gatttgtttg
     3421 atcagatcat cagggaatcc attaggaaac acacgaaatg gagttttgat attcaatccc
     3481 atgatttccc agtggcctgt catcgtatct ttccctacag aaacttcttc taatttcgtc
     3541 gcatagcctt gatgatcttt cactgattcc acaccttcaa gaggacggat cgttcctaaa
     3601 ccaagctgct ctaaatgtgg aacgttcaat cctgcttctt tcgcaatgtg tcctaatgta
     3661 tcagaaccaa catcaccgaa cttatcggca tctggtgctt ctccaattcc tactgaatcc
     3721 atgacgatca gatgtacgcg tttaaacatg attattcctt ctttcttttt atttttattg
     3781 cctttaaaac aaaaagggcg agacgaaaaa cacttcccgt aacccaccct ttttattcgc
     3841 taaaagatta ttttgatttg atatagttct tcccgtttgc ttttggacca gaagctttcc
     3901 ctaagaaaac aaccagcaca atgattgtca gcaaatatgg agcaatttgc agcaatactg
     3961 gcggaatcga agaaataact ggtaattgtg cgccaatgat actcaagctt tgagcaaaac
     4021 caaagaacaa tgaggcaccc attgccccta aaggattcca cttaccaaag atcatagcag
     4081 ccatggagat gaacccttgt ccaacgatcg taccagctga aaagttaccg gaaatcgact
     4141 gagcaaaaac agcaccgccc ataccgccta acaagccaga aatcagtacg cctgaataac
     4201 gcatagcata tacattgata cctaatgtgt cggcagcttg aggattttca ccaactgaac
     4261 ggaggcgcaa gccgaatttc gtcttgaaga tgatcaccca agctaggaca gcaatcaaaa
     4321 tagcacaata agcaggtaag aatgtatttt taaagaaaat tggtccaatg accggaatat
     4381 ctgctaatcc tgggaaagaa aaatacccca gattctgcgt gatgttatca gtttgacctt
     4441 taccatacca tgctttgatc aagaaaacac ctaaagcagg tgccatcaga ttaattactg
     4501 tcccgctgat aatatggtca gcacgcaaac tgactgttgc tactgcgtga atcagagaaa
     4561 ataggatacc tacgacgcca cccactaaaa tagcgatcca tggtgtcata gaaccaagtg
     4621 atggactcat cgtcaagttg aaaacgatgg aactaaaagc acccatgacc atgattccct
     4681 ctagccctac gtttacgatc ccgcttcgtt cagaaaaagt tccgcctagt gacgttagaa
     4741 ttagcggtgt tgaataaacc agtgtagaag taaggatcaa ggcaattaat tcgatagtcg
     4801 acattatagt tcgccccctt catggttttg attcgttggt ttcgattcta ttgtcgcaac
     4861 gatctcttct tgttttttcc cttggaagac tttagccatg acgaaacgaa tcaggaagtt
     4921 gatcccgaca aagaagataa tcaaagcaat cgatacattg acgatctcaa acggaacacc
     4981 tgctcctgtc tgcatgccaa gtccaccgat cttcaaaata ctgaacaaga tcgctgataa
     5041 aagaataccg attgctgatc cagaaccaag caaggaaaca gccattccgt caaatccaat
     5101 agatagcgaa gtcgtttgaa caaagaagtt ttgatacgtt cctaatcctt caaccacacc
     5161 gccaagccct gccaaagcgc cagaaatgac catcgacaaa acgatcgtac gtttactact
     5221 cattcctgca tactcagaag caaaaggatt caatccaaca gaacggattt caaatcctaa
     5281 agtcgttttc ttcatcagca gccaaacgaa aataagcgcg atgattgcta aaaataatcc
     5341 tgagtttacg cgagagccgc caaaagcttg tgttaaccat ggtaaccgca gtgaggcatt
     5401 ttgactgatc atattggaag agtccaaact tgagcgatag ctttcaggca tcacttcatg
     5461 taaagcaaat gtgcttacgt ataataagat atagttcatc atgattgtta cgatcacttc
     5521 acttgttcca aaaaatgctc gaagtaaacc tggaacagcc gctgccatcg caccagctaa
     5581 ggcacctaag atcacgcata acggaagcaa aacaactttt ggcagatctg gaaacgcaag
     5641 tgccacccag acactgacga tccacccgca aagtgcttgt ccagaaagcc caatattgaa
     5701 aaacccagct gagttagcta cagaaaaacc taatgctgta aaaatcaacg gcgttgcttg
     5761 ccttaacgtt tcacctatcc ctctttgcga accaagggct gcatctagca tagaagaata
     5821 accttggatg ggctgaaaac caaaagcagc catcaatatc gcgcctaaga tcaatcctaa
     5881 aacaactgaa agtacaggca ctaataaatt acggaatttt tcgctgcgat cattcatgtg
     5941 cgccagcccc cttttctaac tcttttcgtg cttcttctaa ggtatagccc gccatcaata
     6001 gacctaattc attttcagat gtttctttcg gatcaacgat accgacaatt tctccttcat
     6061 ggataacagc aatacgatcc gaaacattta aaatttcttc taattcgaaa ctgatcagta
     6121 acactgcttt gtatttgtcc ctttgttcga tcagacgttt atgaataaat tcgatcgcac
     6181 ctacatccag tccacgagtt ggatttgcaa caatcaacag atcaggatct cggtcgactt
     6241 cacgagcgat aattgctttt tgctgatttc cgcctgataa agcttttgcg ggaacaagtt
     6301 cattcgttgt tcgaacatca tattcttcga tcaattctct ggcatgttca ttcattactg
     6361 aataattcag catgccgttt ttactgtaag gtttttgatg ataggtttgt agtgcgatat
     6421 tttcagataa tgtcatatct aggactaacc cgtatttatg acggtcttct ggcacgtgtc
     6481 ctacaccatg ttcagtgatt tttcggggtt ttttattagt gatgtcttcc ccttttagct
     6541 tgatatgtcc gctttctgcc tttcgcaaac cagtcaaagc ttggatcaac tccgactgcc
     6601 cgtttccatc gattccagcg ataccaagta cttcgccagc acgaacctct aagttcaagt
     6661 ttttcacggc ttctaatcca cgattttctt tcaccactag attttcaata gacaaaacga
     6721 cttcttgagg cttcgcttct tttttcatcg ttttgaatga aaccgttctt ccaaccatca
     6781 tatcggctaa ttgttgtgag gttacgtctt taacgttgac tgtaccgatt ccttttccac
     6841 ggcggataac tgtacagcgg tcagctactg ctttgatttc atctaactta tgcgtaatca
     6901 aaatgatcga cttgccttct ttgactaatt ccttcatgat cacgattaat tcatcaattt
     6961 cttgaggggt caatacagct gtcggctcat caaaaatcag tacatctgct cctcggtaaa
     7021 gtgtttttaa aatttctacc cgttgttcca ttccaactga aatatcacga acgtaagcat
     7081 ctgggttgac tgacaatcca tactgttcag aaactttttg gatctctttt cgtgcttttt
     7141 tatggtcaag catccctgcg cgactaggct cgcttcccaa cacgatgttt tctgttacag
     7201 taaaggcatc aacaagcata aagtgctgat ggaccatccc aatccctaat tggttggctt
     7261 tggtcggact tgtgatcttt acttctttcc cacgcatcaa aattttccct gatgtcggct
     7321 ctaataatcc ggaaagcacg ttcatcaatg tagatttgcc agcaccgttt tctccaagca
     7381 acgcatgaat ttctcctgcc tttacttgta aattgatgtt gtcatttgcc ttgaatgttc
     7441 caaattcttt ggtaatattc tgcatttcta tgacaatatt ttcttgaacc accttttttt
     7501 cacatcctaa cgatttctat ctagggcaac gattgactaa gattcacgct tagtgagcgt
     7561 tcttcttttg aaacaaacat tacttcaggg agttgttccc ttatccaaac tgtcacaagg
     7621 cagcttggat aaatgaacaa ttcttgttct aatcaaacca tagccttgtc aaacatgtgt
     7681 ctaacaaggc tatagaaatg gttttagttt tcttcaggag tttctggaac ttttacgtca
     7741 cctgaaatga ttttttcttt tgcttcatca actgcttttt tcgcttcgtc agaaagttgt
     7801 ccttctgtta gaccaacacc gtcttctttc aatccataaa cagtgtgttc gccgccaggg
     7861 aatttaccgt ccgctgattt ttgtgccaaa tcttctacta ctgttccaac tgctttcagt
     7921 gtagatgtca atgtgaagtt tttcttttcg ccgtttaatg tgtattcgcc ttcgtctgat
     7981 tggtcgcggt caacaccgat aacccaaact tttttgtcgc ctgattcgtt caatgatttt
     8041 gcttcttgga atacaccgtt acctgtaccg cctgaagcat ggaagatgat atctgcgttt
     8101 tgtgcataca taccttgagc aattgaacgt cctttatctg gagcagagaa gtcaccagca
     8161 tattgattca atactttgat ttcttttcct aattctttcg cacctgcatc aacaccggct
     8221 ttgaatccag catcaaaacg atcgattact tcacctttta caccgccgat aaagccgact
     8281 acgtttgttt cagtcgtata agctgctgca acacctgcaa gataagaagc ttcgttgtct
     8341 ttgaaagttg cagatacaac gttatctaat ccatcgatga catcatcgat aataacaaag
     8401 ttcgtacctg tattgtttgt tgcttgttct tggatcgctg gttttagttt ataaccaata
     8461 ccaaaaatcg ttttgaatcc agcattcaat gcttggtcaa tatttgggat gtaatctgat
     8521 tcatttgaag attggaaata ttggaagcca tcatttcctc gtgaaagtcc ttgatctttc
     8581 ccccattttt ctagtccttc ccaagctgac tggttgaatg aacggtcgtc tacaccacct
     8641 gtgtctgtga tcaatgctgc agttgttgtg tctgatgagc ttttgccacc ggatgaatca
     8701 gtagagccgt tgttgccgca tgcacctaaa aataaccctg ctgctagtgc gacagcacct
     8761 aaaccaaata attttgcttt tttcatttct gtaaagcctc ctaaaataat tgttttattt
     8821 ttcaacagtc agctgactga atgaacatga gttatagatc tttttcagta aatgcataag
     8881 gcagcagtgc acgaacggtc gttgccttcg tcacaccatt gtctcctact agcgtaaccg
     8941 gcatatcagg ggaacagaat tctgccatta cttgtcgaca tgcaccacaa ggagagatag
     9001 gatcgggggt gtgccccgct acgactagat gcgtaaatgt tttttctcct tcagatactg
     9061 ctttgaaaaa agctgtgcgc tcggcacaat tcgttaatcc aaatgatgca ttctcaatgt
     9121 tgatgccttg ataaatcttg cctgattccg ttacgagaca agcgcctacc ggaaaatggg
     9181 aataaggaac ataggcttta tctaacgctt caatagctgt gttgatccat tcttgtttga
     9241 gttctgccat gagcttctcc tttgaaaaaa ttaatagcct tcaccagttg aaccagatac
     9301 gattgctact cctgcgcttg ttccgattct tgtagcacca gcttcgatca ttgccattgc
     9361 ttcttcttcg ttatggatac cgccggaagc tttgacaccc atttctggtc ctacagtttc
     9421 acgcatcaaa cggacatcct ctacctttgc tccgcctgtt gagaaaccag ttgatgtttt
     9481 gacaaaatca gcgcctgcag cttttgctat ttcgcaagct cggatgattt cttctctatc
     9541 taataacgat gtttcaataa taactttgac aagcgcacga tcttttgctg cttcaacaac
     9601 tgcttcgata tctttttgga cttgttgatc atcctttgat ttcaaagcac cgatattgat
     9661 gaccatatcg acttcatctg caccgttatt gatggcatct gttgtttcaa atgcttttgt
     9721 ctcgggtgtg tttgctccta gaggaaaccc gattactgta catacggcta ctggctcacc
     9781 ttgtaattgt tctttcgcta gcgcaaccca tgctggattg atgcagacag aataaaaatg
     9841 gtattttttt gcttcttcga taatttgcat cacagcttct ttagatgcgt ctgctttcaa
     9901 aatagtatgg tcgatcattc ggtttagttc catttctttt ccccttttct ttaaaacggt
     9961 aaagtccgct tttttccttt attctgtgat gatgtcatga atcaacgttg gcttcattgc
    10021 cgattcacca atttcaatat ttttatataa taattcaatg acagaagtga tttcttcgtc
    10081 gttactatag atcgtcaata gtgactcacc ttttgtcaca gtgtcaccaa tttttttatg
    10141 cagtttcaat ccaacagcgt gatcaatatc ttcttctttg gttttacgcc cagcacctag
    10201 catcattgca gcaattccta attcatttgc cacgattttt gacacaactc cgctttgttt
    10261 agcaggaagc tccattgtgt agcgtgcagt caaaattcgt tctggatgtt ccacgatcgc
    10321 tgggtctccc ccttgatttt ggatcatttc tttgaatttt gctaacgctt tacctgattc
    10381 cagagcttct tgaagcaacg ttcttgcttc atcggttgtc ttagctttgc cagcaagaac
    10441 aaccatttgg cttcctaagg cgtagcacat ttctactaga tcttctggac catttccttg
    10501 taatgtttcg attgcctcga caacttctag actatttccg atagcttcgc caagaggttc
    10561 tgacatatct gagataaccg ccactgtttc tcggttagcc agtttcccaa tgctcgtcat
    10621 ggtctttgcc agacgtcttg catcttcgat gttcttcatg aaagcaccgt ctccagtggt
    10681 cacatccaaa acgattgcat ctgctcctgc cgcgattttt ttactcataa tcgaactggc
    10741 aatcagcgga agagaatcaa cggtcgctgt gacgtcacgc aaggcataca attttttgtc
    10801 cgccggtgcc aagtttcctg attgaccaat taccgctact ttactttcat taacaatgcg
    10861 tacaaattct tcatccgaaa gctcgatctg aaatccggga attgcttcta gcttgtctag
    10921 tgtaccacct gtgtaaccta gaccgcgtcc agacatttta gccactgtca ctccgacact
    10981 agctactagg ggggctaaaa tcaatgtggt cgtgtcacca acgccacctg tcgaatgctt
    11041 atctaccttt attccttcaa gagaactcaa atcgatgaca tcaccagaat tagcaatcgc
    11101 caatgttaag ttcgtgattt cttcatttgt catgtcttga taaaaaattg ccatcaataa
    11161 tgcactgact tgataatctg gaatctttcc attcgtatag ttcgtgacga tgtactcgat
    11221 ttcctctttc gacagctcgt ttccatccct ctttttttca atcagatcca ccattcgcat
    11281 tgcctgatcc tccttgtctt cattcggtat tatacattat ttttaggaaa ggtaaaccac
    11341 tttagtaaac tggtttacta aaaataaaaa aagcactaac tagcctttgt aaatgcttac
    11401 ataagcaata taccgccgag ttagcccttt gtcaatactt ttacgctctc acgaataatt
    11461 aattttgttt cgaacacttt gttcggaact tttctttgag gaaattcgat cgtatcgatc
    11521 aggaaacgtg cagcataaaa tccaatatca aaacttggct gctgcactgt cgtcaattgc
    11581 ggaatcatgt attctgacag ctttaatcca tcgaatccta tgactgagat atcttctggg
    11641 atttttttgc ccaattcatc gatcgcctga tagcatccga tcgccatggc atcattcccg
    11701 caaaaaatag ccgtgatatc tttatgtttg agtaattttt ttgatgcagt atatccacct
    11761 tggacagtca actctccatg ttcaacaaat ttcccattaa aaggcaggcc atgatctttc
    11821 agcgcatgac gatatccgtt gaaacgttct tctaattgat agtatccagt ggtttccttc
    11881 agcatgccga tcttcgtatg gccattttca ataaacagcg aaacagcttg ataggcgccc
    11941 tcatattcct ttacgataag ccgtccgtta tcccgacgat tcaatccgcg atcgattaaa
    12001 atgattggca ttttttgata aaaaccgctg tctaatgcat atttctcagg taaaatattt
    12061 ggtgtggcca gcaagattcc gtccactgag cggtgggaaa gctcttgtaa atacttgatc
    12121 tcattttctt ggctatgacg agaattgcat aacatgatca catagcccaa aggattcaaa
    12181 tagctctcta caccttcgat cagtttggag aagaagaaat cagtgacatc agggacgatc
    12241 attccgatgg tctttgaatg tcgattgatc acatttgaag cgaaataatc gggcttataa
    12301 tgatgttcat tcacgatcga caacactttt tttcttgtct tatcactaaa tctgcttcct
    12361 ttgttattca aaatctgtga gacagttgtt actgatacgc ctgccatttc tgcgatatct
    12421 ctgatggtca gcttcatcct attggctccc ttctatctaa attccctatc agtgtaccaa
    12481 aacccccaag aaaagaatag agaaaaaagg aaagtatgtt ttttgataaa aacctccttt
    12541 atctaccgac ttggcagcct tctatacaag ttctgtatct tatttatttt atcgtaaaaa
    12601 acaacgactc tgttcctact atttcttttt gaagaaaaaa tagttctttg tttttaaaaa
    12661 cttctcaaca aaaaaagaac tgatacgtac ccgtacatgc acgcccagtt ccactctttc
    12721 gtcgctccac tatttttcag ctagttattc tttcacactt ttgatgatat aatatccttt
    12781 atcttttgtg acgatttctg cattaccaaa tgtttcttcc attttctttt gcgcactcgg
    12841 cgctccttgt ttcttctgga tcacaattgt cagtgtaccg cccttcttca accgaggata
    12901 tgcgcctgtc aaaatttcat gtacaacttt ttttccagca cggatcggcg gattgcttac
    12961 gatagctgca taggtttctt catgaactgc ttcatagata ttcgattgat ggatatcaac
    13021 atgttcgatt tggttacgct ttgcgtttcc ttgcgctaga cttaccgctc gttcattaat
    13081 gtcgaccatt tcaacaaacc gctgtgtcgc aaatgccaaa gctaacccaa tcggtccata
    13141 cccgcatccc acatctaaga tctttccttc atcaggtaac tcttcccaat cgaaagcatc
    13201 aatcaggaca cgtgaaccat aatccactgt ttcacgtgaa aatacaccag aatctgttac
    13261 aaattgaaag gttctgcctt ttaactcaaa agcccattgt tcaaaatcgt gcgccgtatc
    13321 tgggcgttca gaatagtaat gatttgtcat gtcttccatc cttctcattt ctttcatgac
    13381 aacagtctat ccttttatgt atccgtgcgc aagtcaagaa taagactccg tttctttcta
    13441 ttataaagat tatacaaaaa gatcggcaat ctctcttaag tggtggggtt tgcgtttatt
    13501 ttgtgttaaa atagttccat tgatgggaga acgcaggagg ctaatatgaa tgatccaatg
    13561 aactactacc gtgtggcaag agaagaatgg cgagaatttt atcgtaatgg gcaagcacct
    13621 ttgacacaag atgaactaga taatatcaaa agtttgaatg accgcatttc catgcaggat
    13681 gttcgagata tctatgtacc tcttgctcat ctgatttatc tttatatgaa agaatttgaa
    13741 tctttgttct tgagtaaagg tttatttctt cataaatacg tagaagtacc gccttttatt
    13801 atcgggatcg ccggaagtgt agccgttgga aaaagtacaa cagccagact gctgcagacg
    13861 attttgtctc gtaccttcaa acgaagaaac gttcagctga tcacgactga cggatttctt
    13921 tatccaaatc gtatattaaa agagcgtgga atcatggacc gtaaaggatt tcctgaaagt
    13981 tatgatatgg agatgctgat cgatttcttg aaccgtgtaa aatctggtca ggaagagata
    14041 aaagtacctg tttactcaca tgatgtgtat gatatcattc cagacaagta cgaattgatc
    14101 cagcagcctg atatcttgat cgttgagggg atcaatacgc ttcaattacc tgcgaatcag
    14161 caaatctacg tcagcgactt ttttgatttt tctgtctatg tcgatgccga tcccgtgctg
    14221 atcgaaaaat ggtatttgga tcgttttggt gcgttactgg atacggcttt ccaaaatcca
    14281 gagaattatt attatcgttt tgctatcgga gatcgtgaag aagcctttaa aatggcaaaa
    14341 gatgtatgga aaaatgtgaa tttgaaaaat ttacaggaat atatcttgcc tacacgagga
    14401 agagccgata taattctcca taaaactgaa aatcatatca tcgattcgat tttcatgcga
    14461 aaatattagc attttaaaat agtttgacta ttttgagata aaatttaaag ggataggggt
    14521 aataaatttg acaaacgttg ccgatatgac gaacgttgaa aagattatcg tattagacta
    14581 cggtagtcag tataaccaat tgatcacaag acgtattcgt gatcttggag tcttttccga
    14641 attattgagc caccgcatta ctgcagaaga agtaaaagcg atgaatccaa agggaatcat
    14701 tctttctggc ggaccaaaca gtgtatatga tgagaatgca tttgggatcg atcctgagat
    14761 cttcaattta ggtattcctg tattagggat ttgctatggt atgcaattga tcacgtacaa
    14821 acttggcggt tccgtagagc ctgctaaaaa ccgcgaatat gggaaagcct tgttagaagt
    14881 aacaagcgat caagctgtct tgtttaaaga aacaccaaaa caacaaacag tatggatgag
    14941 ccatggtgac ttggtaacta aagttcctga aggctttgaa acagtcggaa caagcaaaga
    15001 ttgtccgatt gcttctatcc aagacacaac acgtaatttt tatggtatcc aattccatgc
    15061 agaagtacgt cattccgaat acggaatcga attgctgcgc cactttgcct ttgatgtttg
    15121 tcactgtgaa ggtaactgga caatggataa ctttatcgat atgcaagtag aaaaaatccg
    15181 tgaacaagtt ggtgacaaaa aagtcttgct tggtctttca ggtggcgttg attctagtgt
    15241 cgttggggtt cttttacaaa aagcgatcgg tgaccaattg acatctatct ttgttgacca
    15301 cggtctgcta cgcaaaggcg aagcggaaca agtaatggaa agtctaggcg gaaaattcgg
    15361 cttgaacatc atcaaagtca atgcaagaga tcgcttctta gacaagttga aaggtgtatc
    15421 tgacccagaa caaaaacgta aaatcattgg gaatgaattc gtatatgttt ttgatgacga
    15481 agcaacaaaa cttgctggtg aagaaggcgt ctctttccta gcacaaggaa cgttgtatac
    15541 tgacgttatc gaatctggta cagaaacagc acagacgatc aaatctcacc ataatgtagg
    15601 tggactgcca gaagatatgc aatttgaatt gatcgaacca ttaaacacat tattcaaaga
    15661 cgaagtccgt gaattaggga ctcagttagg catgcctgat tcaatcgttt ggcgtcagcc
    15721 attccctggt cctggtcttg gtattcgtgt tcttggcgaa atcactgaag acaagctaga
    15781 aatcgtacgc gaatctgatg ctatcttgcg tgaagaaatc gcagctgctg gacttgaccg
    15841 tgatatctgg caatatttca ctgttcttcc agggatccgc tctgtcggtg ttatgggtga
    15901 cggacgtact tacgactaca cagtcggtat ccgtgctgta acttcaatcg acggaatgac
    15961 cgctgacttt gcccgtatcc catgggaagt cttgcaaaaa atctctgtac gtatcgtaaa
    16021 cgaagtagcc cacgtcaacc gtatcgtgta tgacatcact tctaaaccac cagcaacagt
    16081 ggagtgggaa taaagagcca acataatatg gagggggaat ccctataaat caacattctt
    16141 caatagctaa aatgcttgat tttcattaaa aataagatgg ttccctacca aaagagacta
    16201 taatataagt taatttgttt cattcttatc ataaaaagaa gttctcgaaa aacgaactgt
    16261 tttccgagaa cttttttcta tccatctaag tgctcaaatc atattcacga cggaattcta
    16321 aggttaccct ttcttaaaca aactttatgt tatctgcaca actttttgca attttttcat
    16381 tgcttcttac gaaatcgttg aactctcttt agcataatac aaagtgtaga tgaagtctgg
    16441 tgctttactc gttttggtaa aaatgactgg aaatcgtttc tggttatttt tggcaaaggc
    16501 gaggtgcagg ttcgattcct gccttctacg ctaacgatgt ggctgaaggg aacctccgat
    16561 cttgaatcga aacgatccgc agtctattgt ttcaccaatg aaacaaaatc aacgttcgtc
    16621 caagcatttc ctgaagcctt gctataaacc ccaatttcac aaacacctgt tgtaattgga
    16681 atgtctttga ttgtaatttt tgtccattca cttgttttaa caaccgcagt atttagttcc
    16741 gctccaccat aatttttgat atataactga ttagtatcaa actctccccc agacattaca
    16801 taggcactca atgtgtaagt tcctttgctt atgccactaa cattttgata agctgatgcc
    16861 tcgtatgctt gatcctgcca aaaagttagt ttgtagtccc caaaaaaagt gcccgcttcc
    16921 gttttgatcg tacccttagc ggttccttgt tgaaaccagg tttgccaatt ggaaggactg
    16981 gtcgttggtc catcgctttc gaaacttgca tttttaacta gatttatttg cgcccctgct
    17041 tgtttaaacg cacgcaagct tggcaaggcg tttccagcaa aatcaaacat cgcttggttg
    17101 tcccattcgt ttcccatagt ggattgaaca ctgagataat tcatacctgc ttgtgttgcc
    17161 caactcacat ttccgttgta ccaaagaggt tcccaataga agaagcccgc cccgcgatta
    17221 ttcggtactg cctgaatccg atcggcaaga tccagcaaat actctgcttg tcctgtcggt
    17281 gttggttcat atccccccgc agctgcttcg gcttcaccaa aagcatttgg catagtatcg
    17341 gcattcgctg ttgtatgccc ataagctgtt tcaactacga tcacttcttt accataccgt
    17401 tggctgatcg cattcatatt ggtttgaagt tcattcattg aaccgtgcca atacggataa
    17461 tacgacactc caattaaatc aaatgacaca cctcgactcg tgatttcatc aaaccaccaa
    17521 gtaaatgctc ctgtatcgcc accatcagct aagtggagca ttactggagt tgttttaggt
    17581 tgagtgtctc gaacagcttg gataccagac ttcaaaaaag cagccaatcg atcaaattct
    17641 cccccgcctt ctccccaaga cttgccataa ggccagagca ttccgctatt cagttcattg
    17701 ccgatctgaa ccatatcagg ataaacgtcc agctgcttca tttccgacag aacgttcttt
    17761 gtatagtcgt acaatgccgt attcatttct tcaaaggtca gaccttgcca tgctttagga
    17821 agattctgtt tcccaggatc aacccaaaaa tcactgtagt gaaaatccag cagcacctta
    17881 agtcccagat tttttgctcg ttttgcaagg tcaatcgtcg tttccagatc atttgtccct
    17941 gctccataac tttctccatt actattataa ggatcctgcc acaaacgcag tcgaacatag
    18001 ttgacaccat tattttttaa aatctctaag gcatcttttg aaatatcgtc ttcataatat
    18061 tttgcaccag atttttccat atctgacagg atcgaaatat ctgctccgcg aataaattcg
    18121 ctggcagatg cttcaacagt ttttgaaaca gcccccaaag cccctaacac agctgcaaat
    18181 aaaacagctt tacgcaacca aattcccatt ttcattatgg ctcctccttt attgaaattg
    18241 gttcagaata aactgaacag ctttttcagg atctctcgtt aatccgatat attctgctcc
    18301 ttttgtcgca accagcttga ttcctaagcg atcggtgtct tctttgatat catcatagta
    18361 agaattgact tgcggtgcta agaccaccat atcaaaatcc tgcagcattt cgtaatgaga
    18421 accataagca cccgccattg cactgatcgg tgctttcgat tcagatgcac ctttttttaa
    18481 agcattagct aacatcgcac ttgtccctgc gccagcacaa agtaccaaca cattgatttc
    18541 tttatttgca tcgctgataa cttgactcgc tgccacaggt acttcctcac gtacatcggc
    18601 agaagcacct tcctcggttg cgatcacctc tgctttttgt gcttctttct ctactaagac
    18661 agcatcgtat gctttgcaaa atggtagata gatcaggaag tcaacgacga ttagaaccgc
    18721 tgctagaaca aaagctaaga tacccatgcc cgttcctaga accaaaccga tcggtgctgg
    18781 agttgcccaa ggcaaaacgt acataaagct gttcatgccc aatacatcga caaaaaactt
    18841 gaacaggcaa acgtttacaa caggtgttaa caaaaatgga ataaaaaaat aagggttcaa
    18901 aatcatgggt gccgcaaata gcaatggttc gttgacagcg aaacttactg gaataaagga
    18961 agctttccca actgccttca actgcttaga cttagcaaag agtaggaaga taaaaggaac
    19021 aaccaatgtt gccccagttc ctcccatagt tcctacaaaa ttccctaaac ccaccgtcag
    19081 tactttagaa gcttgttcac cggaattgaa caattgcaga ttggtttcca cattggcata
    19141 gataattgcc gcaattgccg gctccacgat cgacggtcca tgaacaccga caaaccaaaa
    19201 cagcgccatc gctccccaga taatagcgat tcctaagtac ccgtcagctg ctgtaaatag
    19261 cggctgcagc accgtaatga ttgcttctgc aaatgagatt tggaagattt gtcgaatgcc
    19321 taaatcaatt aaaacggccg caaatactga aaaagagaaa ggaaaaatat ctcggaaggt
    19381 ttgcgaaatc gttccaggaa cttctttagg cattttaatg gtaatatctt tcagcacaca
    19441 aaacttatat aaattaactg ttaaaaatgc agcgacaaag gaagccagta gtccctttgt
    19501 ccccatatat tccgttgaaa atccaccttc aatcggagag acgcttaaca acaggaaact
    19561 taccacagag gccagcatca cagaggtggt gttgattact ttgtttttcg gtaagcgacg
    19621 attcatcgaa tcagccaagc agcgagcagt ggttgctgaa acgagtaacc ctacaacacc
    19681 catcgaataa ttatagactt tccatagcca tgtggagaca gattcaggcc attggtaacc
    19741 aaaaatctca ggaaccgcag caatcaaaat gaaaatgctt gaaaataaaa tagctggcat
    19801 cgctgttaaa aatccgtcac gaacggctcc taaataacta ttacgtgaga ttttttcaaa
    19861 aaatggtttc cctttttcga tttgtttaat gattccatcc atagcgttct tctcctatcc
    19921 ttgattctgt ctgtacagta caataaagtc ttgaatcaga tctctcagca ataaagtggt
    19981 cattaaatga tcctgcccat ggataaaaat gaatcccatc tccatctctt tcccagatgc
    20041 ttcttccgct aaaatttttg tttgcgaatt atgtgcaagg gttaaatttt cgtcggcttc
    20101 tttcaacgcc gaatcaacat gttcaaagtt tccttgtctt acttctttca ataatttcaa
    20161 taatgtactg cgagcgtcgc ctgaatatgc aacgatctca aaaccaagca tttgtaattc
    20221 ctctttattc atcttttctt cctccattat cacttattaa ataatcgttt tggtttcact
    20281 gaccaacttg taccagtaag ctgattcttt tgggtaacgc ttctgggttt caaaatcaac
    20341 ataaaacaag ccataccttt tgttataccc atttgtccat gagaaaagat ccataagcga
    20401 ccacaaaaaa taccccttca catttacccc ggctgttatc gccttgctta aggattccaa
    20461 atagactctc aaatagtcga ttctcggctg atccataata atgccatctt caaactgatc
    20521 tttatacccc atcccgttct cagtgatata gattttgttg taatgtgggt aatcgctttt
    20581 aattcgcagc aacaagtcat atagaccttc tggataaatc agccagtccc aatcagttcg
    20641 aggaattcct tctttataaa tacgttctcc gattcccttc actttataaa cagaggtccc
    20701 tttttcaccc gtaccgttat gatggattgc attttctcca tcgtaagctt tgacaaaatg
    20761 gcattggtag tgattgatcc ctaagtagtc attacgcgtg gatgcttttt tcaattcaac
    20821 gaaatcttct tcaggaaaat gatagctggc ctgatttgct tcacatatct catcaagagc
    20881 agtcaaggtt tcagttgaat aatagcctaa ataggtagca tctaataaga aacggatcga
    20941 caatgcatcg tctaaaaagg cagcatgctt atcttccggc gcgtctgttg ccgcatattt
    21001 cgtctctaat gagtgaacta caccaatttc acccggaagt tcgttttctt tgaaatagtt
    21061 cactacacgg gcatgagcaa ccatcatatt atgaagacag gcgacgatct ttgtaaaatc
    21121 atatttgatt cctggtggga aaacacctaa caaatattga ttggttgcaa ctggatagat
    21181 ttcattaaag gtactccaca ccttgacttc tttgaattca tgaaaacaaa aaatggcata
    21241 ggaaacaaat gcttctattg tttctcgatt caaaaaatct ccatggtcaa acaaacgttt
    21301 aggggtatca aaatgatgca acgtcacaaa cggcgttacg tttcttttct tacattcttc
    21361 gaacacacga tgataaaaag cgattccttc tggatttggt ttcccagctc catcaggaaa
    21421 aatgcggctc caagcaatgg ataatcttag accatttaca ccaaaacgtt cacacaattc
    21481 aatgtccttt gcatattgat gataaaaatc acttgccgga tcgggactga atcgcccttg
    21541 ttcctctaaa aagtcatccc aagcaacagc gcctttgcca ccttccttag tggctccttc
    21601 aacctgatag gctgctgtgg ctccgccaaa aataaaatct tctggtaatt taagcatgta
    21661 catctctcct caaatctata atcagttttc gctctttcta actgattgca ttgtaaacgc
    21721 atccaaaaca tttgtcaaca aaaacaaaca caaataatca taaaaaccag aaaacagcaa
    21781 taaaatctat tgtaacaaac ataaaaacca ccctaaatgt ttacttttgt tcgaattaga
    21841 ttgactgttg tccgtttata tgatagatta tccttaaatt agaggtgaag acaatgttga
    21901 aaaaagaaag attgcttact atcgtagaaa tggtcaataa aaaaggaatc cttaccgtta
    21961 atgaaatcat caatcaatta gatgtttctg atatgaccgt tcgcagagat ttagatgaat
    22021 tagaaaaatc gggcaagctg ctgcgtgtcc atggaggggc tcaaagtctt tcctacactt
    22081 tagatcaaga actttctcac actgaaaaag ccactctgca gattcaagaa aaaaaagaaa
    22141 ttgctgccac agcagcctcc ttgattcacg agggagaaac gattttctta ggaccaggta
    22201 caacaatcga actattggcg acgcatttac tgaacaagaa aattcggatc attacaaata
    22261 attatccagt ttttgatatt cttgctcaag cagatggacc agaaattcta ctgattggcg
    22321 gtgatttccg gaaaaatacc ggcgcatttg tcggaccgat caccaacgac aatttaagaa
    22381 gactaaagtt tacaaaagca tttatcagtt caaatggcgt tcacaacgaa gcgatctcta
    22441 cttacagtac cgaagaagga gaagcacagc agatcgctct aaataattcc cgtaagaaat
    22501 atctactcgt tgataataaa aaattcaatc gagatgattt ttatgtgttt tataatttgc
    22561 atgatttcga tctgctcatt acggatcgcc agatctcgcg ggaggttcaa gagcactatg
    22621 cacaatatgt cgatctgcaa attgctgatt caaccgaaaa taccactata ggagcgtaac
    22681 tatgaaaggg attatatttg atttcaacgg aacaatgttt ttggactctc atctgcatga
    22741 agctgcttgg ctgcatatga tacatgatca tacaaaagat ggtttatcag acgaggatat
    22801 tttaaaaaat attcatggtc ggacgaatac ggagattttg aaacatttca tttcagagca
    22861 tttgaccgcc tctgagatcg ccgtattgtc agaagaaaaa gaaagctatt atcgtatgct
    22921 ttgcttgcaa aatgaaaacg agcttcagtt gacaaaaggg cttgaagcga ctcttgacca
    22981 gctgactgac caacagattc cattaacgat tgccacggct actgtcaaag agaatgttgc
    23041 tttttatttt gatattttcg atttagcccg ctggtttgac tgggacaaga ttgtctatga
    23101 cgacggctct ttccctggga aaccgcaacc ggatattttt cttaaagcgg cagacaaact
    23161 gagcttagct ccggctgatt gtcttgttat cgaagatgct tactcaggat tacttgctgc
    23221 caaacgtgcg gggatcggaa caattattgc aattgatcca ttcggtaaaa acaaaacagt
    23281 atttgaacag gcacagctaa ataaagatgg aaccatcaaa gattttactg gtttttggac
    23341 agagcacctg tctgccacat ccgttaattc ttaaacgatt caactaacta ccaaagaaaa
    23401 gcaactgaaa tgcacacctc cttttataaa aaactgccag tgaaacgaca tgaaagatac
    23461 actgctatat ccttagtgaa atatagcagt gtatctttga ctttttcatt cttttaacca
    23521 tatgacgcgt tcatctctat ctataattaa cattaaaaat agtgttttca cattatttct
    23581 caaaccaatc ttaaaaatca tcaggagtac ttttttgata tacttttatt aatgctgggc
    23641 gtaatggtta aaagatttcc actagttcct ctccaccaag ctgagttgaa aaatgcttat
    23701 ctgtaaacaa catagacaaa gaggaaataa acgtttccgt atctttctct gtcccaagga
    23761 tattaattaa ttttcctact aggtaagtat cattaaactt aagatctgcc ttcacatcaa
    23821 aagttttgat aaaagctttt atcatgcgta caattttgtc tttatcgcca taattgattt
    23881 caccactcat atcaatatag tcactaatcc ctgaagaaat aatggcaact gcttcttctt
    23941 tcgccttatc agataattca tttaatgact gggattgatt gatgacttct tgagttttat
    24001 ctgttacgta atctctcatt atcctatcgg catactccgc aaagtaatct tgaaaaaatt
    24061 cttctacagg gtaaccaact tcgttttccc aaccaggaga aatttcatta tcagtactta
    24121 ttaaatctat taaaaaccaa cccgttctct gagtgttgat tctataatat ttttaatttc
    24181 ctatataaat caaaaatatt atagaatcaa taagctaatt aagaaacaaa ctttagaaa
//
LOCUS       DAFGFZ010000036        23843 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000036 DAFGFZ010000000
VERSION     DAFGFZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.036"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(56..790)
                     /locus_tag="LR726_001222"
     CDS             complement(56..790)
                     /locus_tag="LR726_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL6216324.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="LR726_001223"
     CDS             1117..1527
                     /locus_tag="LR726_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL6216325.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="LR726_001224"
     CDS             1520..2221
                     /locus_tag="LR726_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL6216326.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="LR726_001225"
     CDS             2221..3807
                     /locus_tag="LR726_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6216327.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="LR726_001226"
     CDS             3826..4047
                     /locus_tag="LR726_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL6216328.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="LR726_001227"
     CDS             4044..4805
                     /locus_tag="LR726_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL6216329.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="LR726_001228"
     CDS             5185..5694
                     /locus_tag="LR726_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL6216330.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="LR726_001229"
     CDS             complement(5769..6308)
                     /locus_tag="LR726_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL6216331.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="LR726_001230"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="LR726_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL6216332.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="LR726_001231"
     CDS             7321..8172
                     /locus_tag="LR726_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL6216333.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="LR726_001232"
     CDS             8200..8835
                     /locus_tag="LR726_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL6216334.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="LR726_001233"
     CDS             8855..9628
                     /locus_tag="LR726_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL6216335.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="LR726_001234"
     CDS             10036..10833
                     /locus_tag="LR726_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL6216336.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="LR726_001235"
     CDS             10811..11224
                     /locus_tag="LR726_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL6216337.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="LR726_001236"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="LR726_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL6216338.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="LR726_001237"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="LR726_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL6216339.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="LR726_001238"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="LR726_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL6216340.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="LR726_001239"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="LR726_001239"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL6216341.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="LR726_001240"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="LR726_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL6216342.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="LR726_001241"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="LR726_001241"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL6216343.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="LR726_001242"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="LR726_001242"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL6216344.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="LR726_001243"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="LR726_001243"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL6216345.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="LR726_001244"
     CDS             21908..22486
                     /locus_tag="LR726_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6216346.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="LR726_001245"
     CDS             22538..23020
                     /locus_tag="LR726_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL6216347.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="LR726_001246"
     CDS             23137..23511
                     /locus_tag="LR726_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216348.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7788 a         4288 c         5119 g         6648 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaatagcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttttgcgaa aac
//
LOCUS       DAFGFZ010000037        23832 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000037 DAFGFZ010000000
VERSION     DAFGFZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.037"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            281..694
                     /locus_tag="LR726_001247"
     CDS             281..694
                     /locus_tag="LR726_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL6216349.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1314..1478
                     /locus_tag="LR726_001248"
     CDS             1314..1478
                     /locus_tag="LR726_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216350.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            1890..2435
                     /locus_tag="LR726_001249"
     CDS             1890..2435
                     /locus_tag="LR726_001249"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6216351.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2436..2753
                     /locus_tag="LR726_001250"
     CDS             2436..2753
                     /locus_tag="LR726_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL6216352.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2910..3074)
                     /locus_tag="LR726_001251"
     CDS             complement(2910..3074)
                     /locus_tag="LR726_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBL6216353.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            3176..3697
                     /locus_tag="LR726_001252"
     CDS             3176..3697
                     /locus_tag="LR726_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBL6216354.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            3684..4157
                     /locus_tag="LR726_001253"
     CDS             3684..4157
                     /locus_tag="LR726_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL6216355.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(4164..4322)
                     /locus_tag="LR726_001254"
     CDS             complement(4164..4322)
                     /locus_tag="LR726_001254"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBL6216356.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4415..5176
                     /locus_tag="LR726_001255"
     CDS             4415..5176
                     /locus_tag="LR726_001255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL6216357.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5396..6715
                     /locus_tag="LR726_001256"
     CDS             5396..6715
                     /locus_tag="LR726_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL6216358.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            6731..7951
                     /locus_tag="LR726_001257"
     CDS             6731..7951
                     /locus_tag="LR726_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL6216359.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7986..8663
                     /locus_tag="LR726_001258"
     CDS             7986..8663
                     /locus_tag="LR726_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL6216360.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            8660..9853
                     /locus_tag="LR726_001259"
     CDS             8660..9853
                     /locus_tag="LR726_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL6216361.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            9868..10194
                     /locus_tag="LR726_001260"
     CDS             9868..10194
                     /locus_tag="LR726_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL6216362.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            10198..10533
                     /locus_tag="LR726_001261"
     CDS             10198..10533
                     /locus_tag="LR726_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL6216363.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            10530..10925
                     /locus_tag="LR726_001262"
     CDS             10530..10925
                     /locus_tag="LR726_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216364.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10922..11293
                     /locus_tag="LR726_001263"
     CDS             10922..11293
                     /locus_tag="LR726_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216365.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11309..11869
                     /locus_tag="LR726_001264"
     CDS             11309..11869
                     /locus_tag="LR726_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL6216366.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11994..12305
                     /locus_tag="LR726_001265"
     CDS             11994..12305
                     /locus_tag="LR726_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216367.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            12329..12520
                     /locus_tag="LR726_001266"
     CDS             12329..12520
                     /locus_tag="LR726_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216368.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            12520..16368
                     /locus_tag="LR726_001267"
     CDS             12520..16368
                     /locus_tag="LR726_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL6216369.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            16369..17241
                     /locus_tag="LR726_001268"
     CDS             16369..17241
                     /locus_tag="LR726_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBL6216370.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            17238..18287
                     /locus_tag="LR726_001269"
     CDS             17238..18287
                     /locus_tag="LR726_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216371.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            18291..19115
                     /locus_tag="LR726_001270"
     CDS             18291..19115
                     /locus_tag="LR726_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216372.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            19133..21193
                     /locus_tag="LR726_001271"
     CDS             19133..21193
                     /locus_tag="LR726_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBL6216373.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            21210..21359
                     /locus_tag="LR726_001272"
     CDS             21210..21359
                     /locus_tag="LR726_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216374.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            21356..21802
                     /locus_tag="LR726_001273"
     CDS             21356..21802
                     /locus_tag="LR726_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216375.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21804..21941
                     /locus_tag="LR726_001274"
     CDS             21804..21941
                     /locus_tag="LR726_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL6216376.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21976..22218
                     /locus_tag="LR726_001275"
     CDS             21976..22218
                     /locus_tag="LR726_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBL6216377.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22234..22431
                     /locus_tag="LR726_001276"
     CDS             22234..22431
                     /locus_tag="LR726_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL6216378.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22442..23458
                     /locus_tag="LR726_001277"
     CDS             22442..23458
                     /locus_tag="LR726_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL6216379.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            23573..>23832
                     /locus_tag="LR726_001278"
     CDS             23573..>23832
                     /locus_tag="LR726_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216380.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFI"
BASE COUNT         8277 a         4037 c         5275 g         6243 t
ORIGIN      
        1 gtgtttgaca aagtaacaaa atgcgacgat accaatacaa acgggaatcc aaaataacag
       61 catgctccac atagtcactc aaccatctcc tatacgcttt attatcaata gataatatca
      121 tttttcgtaa taaagataaa aaaataagta aggcttctca ttaaaatata aaaaagacag
      181 ccgaccactg actgcctata taagagtatt gaaataaaaa gctgctgata taataaattc
      241 cacaagttta ttatatcaca taaaggagcg gtttgacttg atgcaattgt tacgagaggt
      301 agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc ggcgtttgga
      361 gcggatggca ggtcgctctt tgatagatat taagtcgccg attattacgg atatgccgaa
      421 ggcgccgaag cacggcaata aggcagaaga cgcaatcatt cagatgatgg atatagaagc
      481 ggagagagac gcgattctag cagccttgat ggctcttagt ctgattagtc gtcagatact
      541 ctactatagc ttttgtgtgc cagatagctt ctcaaactac agaattagcc gtgaagtggg
      601 ttattcagaa agaagtatac aacggatgaa gtcggaagct ctaatagagt ttgcagaagc
      661 atataaacac ggaagaataa ttgcttataa ataatttggc ggctttttgg cggaatgatg
      721 gcggttttta gctatttacc agtgatatta tggtagtgtc gaaagattag tgataggtct
      781 aagacaaaat aataataaaa ggaacatcgt tttattattg tttcacaatt aagcttcgat
      841 agacagcaac ggaaatatta agaataagga tgtgaatttt aactccttct aaattgttct
      901 tattatctat catccgttgc tgtctattgt ttttttaatt attcacacga taactaaagg
      961 tgagcgaaga gaaatgattc cattaataat ttcaattttt gcgctttgtc ttaatgtcta
     1021 tatgattgga tttaaaaatg ggcaaaataa aagatagtag taactaagaa agaaattttt
     1081 atattgtcac tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg
     1141 caactatgca atgttcgatt cattgccagt gactttggca accgaggcat cggcggttta
     1201 aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat cgtgtaggtt gctattacat
     1261 attagatcac tcgttgagtg gtctttttat tttacataaa ggaggtaaca acgatgtata
     1321 gaccacaata cttagaacag aagtatgaag taatcactgt gcaaaacggt aacggtgaga
     1381 tagtacgaaa gtatagaaga ccaataaaga gcgatacata taaacgaaag gaaaacaatg
     1441 aagttatttc ttttcgcaga aggagaaaag ccaaatgaga aactactggt atgtatcgct
     1501 aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc gttctgtaca
     1561 aatcaaaggg aagtactcta ttgtcgaaat gctaagagaa gctacaccaa acgaagtgga
     1621 taaatgcaag ctgatatatt gcggtcatgg ctattggaaa gacgagtata tccaacaaaa
     1681 cattgagagg tggatagata gatgagttac ctcgaacatt tgaaacgttg ctacatgcat
     1741 tctaagaata aacttcctga cagctacact gtagatgatg ttgctataca tgtattgaag
     1801 actgagagcc atagcagtcc tgatggtagc agtaaggagc agacgctcgc gtggttcaag
     1861 ttctttaagt ggattaagga ggaagagtaa tgcacatgca cataggagaa aagatatata
     1921 aattgagacg gcttaataac gaaacaatga acgacttagt taaacaaact ggagtgtcaa
     1981 aatcatggtt atctgatatt gagtctggaa aaaagcaacg cgttgatttt aataagatat
     2041 gtgcaatagc caaacattat aaagttagct tagaatactt tcgggatgat tatttggata
     2101 gtgatgaaca cgaggatatt attcctttga caggcgaaac aagagttaaa tcagtgaaat
     2161 cgcttgaaca aagcctaaag tatctgaata acaaacaaga acctaacctt ctagagatca
     2221 aactaaaaga tactgactca gtaccagagg tttggtacaa gggtgagagg ttggatgaat
     2281 tgcctaaagg attagtagat gtctcgtatc attggaagac tgatgatttt actaatgatg
     2341 atagaggagc gaacgacatc acgattcaat acttttctag ctttaatgat aagtatccag
     2401 ataagaaaac aatcggacac aagagagata tgtaaatgaa agaagttaga cctagagacg
     2461 aaatagacaa actatacaag accaaacgat ggcgagacct aaggcaagta gtaatagcta
     2521 gggacttcgg catgtgccaa gagtgcaagc gtcgagggcg gaacacaagg ggcacgatca
     2581 tccatcacat agtcgaggcg agggaagacc tgtcactgtt ctggtccgta gataaccttg
     2641 aatgtatctg tgtagcttgt cacaacagag agcatccaga gagatcaggc gggaagaaga
     2701 aaccaaaacc taaatcacat atcgttaaaa tgtattcaac tcctgaaaga taagtttgca
     2761 gcgaaatgaa ggtagccccc ctactctaaa agattaaaga gtaaggcttg agaagaacgg
     2821 tgctgtcctt ccttcgtaaa aagaccgctt ttcaagtttt ttggagaaaa aggaaaaagc
     2881 cgaccaattt aagccggctt tggacgaagc tatttcttag tccatttgtt tcctttttga
     2941 gaagtagggg gtaatcggtc gcctggatca atttttactt cttgtccgcc ttggacattt
     3001 ccaccacgag gacccacttc tttgtaggtt ccttttggtt tgttgtcttc gccgggttta
     3061 tagagttctc ccataggaat ccctccttaa caaatttcgg cacagcactg ccgataacct
     3121 aattataagg attgtgataa cgatttcaat ctatcttttg aaagaaggtg atattatgcc
     3181 gcaaccagcg aagagtgcaa aattacaatt attaaacgga aacccaaata agaagaatac
     3241 cgaagaactc cgcaagcgag cggccgcaga agacaaatta aaaatggcta ctgacaaaat
     3301 caaaccgccg ttatggctag attcgctagg aaaggatacc tttgagttta tcgccgatga
     3361 attgctgtct gtggatttaa tcagtaatcc ggacgtccat acaatggctc tatactccaa
     3421 ttggtattcg caatacgttt ctttagaaaa acagcttcga aaactacaac gagagtacaa
     3481 gttgaactat gcgcttgcga aaaaggaggc agaggcgaga ggtgagccgt ttaatgaacc
     3541 taatgaatta attggtaatc cgctctctcg gcagatggat acagcgtctc ggaatctccg
     3601 ttcttttggc gctgatttag gactatcacc agcagccaga gctaagttag ctattaagat
     3661 ggctgatgat ggtggtgatg acgatgacga cttctaatat tttggatatg tcttacacag
     3721 aacgtgtgga ctattggcaa agctatcttg aggagcaagc ttcttggggt ggctttttaa
     3781 aatgtccata tccggaattg ttaactactt ggtatgcgga acgattaatc gatggaagca
     3841 taccagccag caaagaaaat attcaagctg ctaaacggca tatgcgtgat ttgcagcgcc
     3901 aaggaacaga tgattttcct tggatctttg acgaagaaaa aggtcaccgg cctattagat
     3961 atatcgaaaa aaaatgtaaa ccaactgaag gcgactttgg ttcgtttgtt ttgcaacctt
     4021 ggcagcattt cataattgga tccatgtacg gatgggtaca tcgtgataca ggagagcgtc
     4081 gcttccgcga ggctcttatt tttgttggac gtaaaaacgg taagttttgc cgtttaaaaa
     4141 tcgggcaaaa tcggtgaaaa cctttatttt tggctttctt ttagtaataa ttcgatagct
     4201 ttatcaagca atttagacat aggaaccata gtttcttccg acattttttt taatccttcg
     4261 tataaatctt tatcaattgc gttagaaatt ctaattctgt tttttagtcc gtatttgtcc
     4321 atgtgtgaca ctccttttta ttttactata acatccgatt ccaccgcttg caacttccat
     4381 caattgatgg tagtatataa aagaggtgag tagattgaaa aaatacatta tttacaaaca
     4441 cttaaataaa ataaatggca aaatttatat cggtgtcaca aatgatattg gcagaaggtg
     4501 gaggagcggt gggattgaat ataaaccacc aaagaacgaa acccaacacc acagaagttt
     4561 ttggaatgca atacaaaaat acgggtggga aaattttgat catttgatta ttgaagaaga
     4621 cttaacgatg aaagaagctt ttgagaaaga aaagttttat attgaacttt atgactcaac
     4681 taataagaaa aaaggttata acatcgcaaa aggcggaaat ggcggaataa tatataaagt
     4741 tcaccccaaa ggaatgctag ggaaaaaaca atctaaagaa ttttcttcaa atcacagtaa
     4801 atgggcaaag aaccataaga ataattgtat gacaaacggt gatgttgtat ggggtgttac
     4861 tcacgaacac ccaaaaggca tgttgggaaa gcaccacagc aaagaatcaa ttatgaaaaa
     4921 gaaagcatat agtggtgaaa atgctgtaac ctcaaaacca attgtcgcaa ttgaacggga
     4981 tggaactaaa agagaatttc acagcgctaa gctttgcatg gcttactatt cgattagtac
     5041 atgtgttttt taccgacttt taaaagatgg cgccccttat gtgataaatc ctaaagcgaa
     5101 ctacagaaat aaagaaaaaa tattagctat cgaaggaatt atgttcaaac ataaagaaga
     5161 taccgaggta agttagtaga ttgcgaaagg ttactaacta ccgtaacgcg taggagttga
     5221 ataaatataa tgctcccaag agtgtccgac agcagataat ttgctgaaaa tgtacgctaa
     5281 actgggccag aactgactga ccgatgaaaa tgagggtgac ctccagagtg cgagataaaa
     5341 agctcgcaga taataacaat cgaaaacatc actaatttca ggcctttcca catacatggt
     5401 cgcttatgat gatgaacaag gcgccaacgt ttacgtattg gcaaatgctc gtgatcaagc
     5461 aagcttgttg tttgataagg ccgcagaaat ggtcaaacaa tcgccggcgc tctttaagaa
     5521 atttggtaag cctaaacgat caagtattaa ttatgctccc gccttttcta aaatggaacc
     5581 acgcgcctca gatagccgga aattggatgg gctaaacact cactttggta tttttgacga
     5641 gatccacgag tttacgaatt acaatctgat caacgttatc aagaaatcaa gaggaaccag
     5701 aaaacagcct ctgatagttt atatcacaac tgctggatat gtattagatg gtccgttgat
     5761 gtcttatttt gagcaaggtg tggactgttt ggaacatttg gaagatgaca tcgatgaacg
     5821 gactttctat tatctggcaa aacttgacag tgcggaagag gctgatgacc caagattatg
     5881 gatcaaagcc aatccgaata tttgtctaat gaattttgtt ggcatgctag atgactatgt
     5941 taaggataaa aaagatccaa aagaatatgc tgactggatt accaagcaat ttaacttgtt
     6001 ttccgatatc gatgagctgt catttgtcga tatgcctacc attaaacgaa acaataaaac
     6061 catcgatatt gaaacgctca aaggtaagaa gtgtgtcggt ggttttgact tgtccgaaac
     6121 ggaagacttt accgcagccg ttttagaatt tccgcttgaa acaggcgagg tattcatttt
     6181 gcaacacaca tggatcccac aagctagatt tgatcgagat aacaatcaag agcgtatcaa
     6241 agcgtgggag aaggtgggag atctaacgat tattcctggt gattacgtca attatgaata
     6301 cgtcttaaat tggtttgtag aaaattcgaa aatctatgac attgtaaaaa tcaattatga
     6361 caaggccaag gcgctacgat taaataaaga actagaaaat gcaggatttg aaaccaacga
     6421 gattcggcaa gggtttctat cattaggtgg gccaatgcaa aacttcaagg aaatgctatt
     6481 ggacggtaag gtgattttca acaattccaa gctttaccga tggtatctat ccaacgtcaa
     6541 gctggtgatg gatcgcaact caaactggat gccgtctaag cagtccaaga gtagaaaaat
     6601 agatggtttt gcagcaagtt tgaacagcca cgccgaagtg ttgaatatgt tggttaatcc
     6661 tgtcggaacc gggaaagtaa cctattactc gatttccgat ttaatgaata tgtaagaaag
     6721 gtgtggagga atgagtattt tagatcgttt gcgttctttt ggccgagcga agccgaaagc
     6781 gagcaaacaa gagtattttt tgaatgaccc gggattgata ccgtatttag tcggaaaaga
     6841 tgaaatatca gaagggattt tttccgtaat tagccgtgta tcgaacgttt ttgcgtctct
     6901 ccctctcaaa atgatagatg tggagtttgg ccaaccggac gactgtcctg catacaactt
     6961 gttgagcgaa ggccctcgat attttacaaa gtttgatttt ttccgggacg tggaagtttt
     7021 gagaaactac caagggaatg cgtatgtgca gattttccga aatatcaatg gagaagtagc
     7081 agatatggcg ttagtaaaac ctggtgcttg ccatccagtg attgatatgg atagcgggga
     7141 gctttactac caagtaactg cgactgacaa aggcagttac aagcaagtta tctatgtaca
     7201 ttacatggaa atgcttcact ttaaacaacc gaggtttggc ggcttggaag gtacagaccc
     7261 cacaaaagta ttaacgaata ccctcggata tgatcgagaa gtccgaaaaa tctctttaag
     7321 tcagcttaaa ggaagtaatg aagggctaaa agttaagttt gctagcaata tggatgaaga
     7381 agctaaaaaa gctacagtta aaaacattgc tgatttttat cgacaaaacg gtggactact
     7441 tgtggaagaa aacggtgtag aaatcgaacg tttacaacga gagctggtag acagcaagct
     7501 tttagatact gataaagtat ctcgctccag aatcgctatg gtctacaatg tgccggagca
     7561 tttcatcggg aataaccagt cgagttactc ctctcaggaa cagctcaata tggaattttt
     7621 gacgtacaat ctagtaccga ccgttaatca atatgaagcg gaactaaata agaaaacact
     7681 atcgagaacc gaaaaagcaa aaggttatcg atataagttt aatgtcgcaa gtttgctaag
     7741 agctgacact caggcaaggg gacaattcta tcaaatcatg cgacgaggcg gagcatactc
     7801 agcgaatgat gtccgaagat ttgaggactt gcaaccgata aataaaaccg gtatggatga
     7861 ttaccatatt tccggagacc tatatccaat cgatatggat ccaacattaa gaaaaacaac
     7921 ctcgtctaaa agcgtagccg aaaacggtta ggttttttta gtttgcaccg aagggaggtg
     7981 gaaaaatgaa aaaagtgacg ttaagcggcg atgtcgtgga taacgatacc gcgtggcttt
     8041 atgactggtt tgggatcaat tgtatctcac cagggaaaat ttctgccgct cttacagaag
     8101 cagcggggga tgaagtagaa cttgatatct catcgaacgg tggggatgtc ctagcggcaa
     8161 gcgaaatata taccgctatc cgtgcctatc cgggaaaggt atccggaaat gttgtgagta
     8221 ttgcagcaag cgctgcaagt gtaatcgctt gcgcttgcga accgcttaga atctcaccta
     8281 cggcacacat catgattcat aacgcatggg tgaccactag tggcaacgct gaggaattaa
     8341 aagccaatgc agaaatgtta agcagtgtgg atgagtctat tgttaatgct tacgagatca
     8401 aaacaggact cgatcggaaa aaacttgctg atttaatggc gaaagatact tggttaaatg
     8461 ctcaaacagc agtagcagaa gggtttgcag atgaaattat gtttgcagaa gcaccagtaa
     8521 cggtactcaa tgcctctcaa ccggttattc caaaaaacgc agtaactaag ttgaaaaatt
     8581 taatactcaa agcggaagca ccgcaagaag aaacactctt acagaaaaaa ctaaaagcct
     8641 taaatggagg gaaaaacgaa tgaatttaga acaattaaaa aatgcgtggg tcgaggcggg
     8701 aagtaaagtc tctgacttaa atgcacaact caatgcagca ttggttgacg atgaaaaaac
     8761 agaagaagat gtagtaagtt tgcaagcaca agtaaaagca gcacgggcta aacgggacgg
     8821 attgaaagag caagtggcaa atatggaagc cgaacaagtc ttaaacgtca aaaaagaacc
     8881 attagataaa aaagatgaaa acttgaaaaa caagtttatc aaagacttta aagcgatggt
     8941 caatggtgat cctgctatta tggctacttt gacatctaat acggatgaat ctggtaatgc
     9001 tatcggattg actattcctg tagatgtgca aacgactatt catactttgg ttcgtcggtt
     9061 tgactctttg caagaatacg taaacgttga aaaagtgacc actaccagcg gttctcgcgt
     9121 ttatgaaaaa tggtctgata ttacaccgtt gactgctttg gatactgaag acggtgaaat
     9181 cccagcaaat gatgatcctg cactttactt gatcaaatac ttgatcaaac gctatgcagg
     9241 tatttccaca gtaactaaca gcttgctaaa agatactgcc gaaaacattt tggcatggtt
     9301 gtctaaatgg atcgcgaaaa aagtagttgt tactcgcaat acaaaaatct tggcagctat
     9361 tgatggaatc aaagcggcac aaaagaaaga tgttacagat gttgatggaa ttaaagatat
     9421 cgtaaacgtc caacttgatc cagctatcga agctacatct atgtttatta caaaccaaga
     9481 tggcttcaat gttttagata aagtgaaacg tgctgatgga tcttacttgt tacaaaaaga
     9541 cgtagcttct gcaactggat ataccttctt aggtaaacca atcaagaaaa ttgcttctcg
     9601 tttcttgcca aacaaaggta cgcaagctac tcctaaatat ccactgtaca ttggtgatct
     9661 gaaagaagcc gttacattgt atgatcgcga aaacatgagc ttgctgacaa cgaatattgg
     9721 tggtggagct tttgaaacag acaccactaa agtacgcgtc atcgatcgct tcgatgtgca
     9781 actagttgat gatgaagcgg ttgttttggc tacttttaca actattgcga acgagacacc
     9841 ggcgaaagtt taaggagctg attccttatg attcttgatc ctaaaatgga tttaggcgaa
     9901 atcaaaaacg cactaaagat tgataccgat gatgacgatg tggaagtaag ccgtgcggca
     9961 caagctgcaa ttgcatacat tagaggggct atcgggaatg ataagccctc tttttataag
    10021 caagaaaatg aaacggttga tctgattaat ttagctatcc tgcaattagc agatcattat
    10081 tacaacgctc gatcagcaac cgtgagtggg aacttgcgag agtacgattt aggttttaca
    10141 agcctaatct tgcaactcaa agcaagttat ttgctttttg tggaggagga gtagcgtatg
    10201 ccccttatcc aaacaggaaa tttaaatcaa cgcatcaagt ttgtccgaga tacgacggtt
    10261 aaggatgagg acgggcaagt tgtcccgact tctacaacca ttcttacttg ctgggcaagt
    10321 gtgcagacac aacgcctgaa cgatattaag acgtcgattg gtacggcttt ggaaggaaca
    10381 ctgacgttca ttatccgcta ccaacaaaaa tcagagctga ccaatgatat gaaagtgcgt
    10441 tggaatggaa aaacgtttga aatcattacg attacgaaag gcgagtttgc gaaggacttc
    10501 acgacaatca ttgcaaaaga ggtttcaaaa tgagtgtaga agtcgatgca accgaagtgt
    10561 acaaagcgct tagggaagta aaagcaaacg ttcaacgagt ggaaagccca gcacttagaa
    10621 aagctgggga gtacgctcaa gaagagttac gacaaaacac accttactgg gatggaacga
    10681 agtcaaacgg taaacgtggt tcgtatatgc aagaacatgc taagaaccat gtggttacaa
    10741 gctcgataaa aaacggattg gtagaagtcg gctatgacaa agatgtttct tggcggatgc
    10801 actttatcga gttcggaaca atcaaacaac gtccaaaagg tttcgtacaa aaaacacaaa
    10861 agcaaatcga aaaacaagta acacaaatca ttgctgacga agtaaaaagg aggctaggac
    10921 tttgaaaacg gcagtatcac aagtctattc aattctgaat agcaatgaaa aaacaaagaa
    10981 cattgatttt tacaccaata gtgttccgga atcagctcaa acagtaccta gccttccagt
    11041 tggcagaatt acagagatat ccggcaacta tgaagatttt gcaagcaaca atcctttgac
    11101 cattcaattt aacgtacagg tagatgtatg ggtgtcaacc atgaaagagg ttgatgcctt
    11161 ttattttgcc cttgatgagg ttatgagggg gaatggttgg caatgcgcat acacggaaca
    11221 aacagatgac gaggacttgg aaggtgcaaa gcggattatc aaacgatatg tagcaaatat
    11281 ttcactaaat taaaaggaga gaaaatagat ggcaacagta ggattcgaga gcgtcatttt
    11341 tggcgtaaaa acaggtgcag gcggcactct aaaagaatta gtagcagata agtcgaaagg
    11401 cggagcgatc gaagctaaaa ttactggatt aggcgcaact tctaacacaa catacgcttc
    11461 aaacgtaccg ttcttcattg caagtaaagg ggtttcgtcg ccaaaagtta cgcttgacgt
    11521 ggcagactta atggataacg gcatttacag cgaaatcatt ggtgctaaaa ccgtggatgg
    11581 tgcaaatgta attggttcag aaactgaagc gccttacgtg tcggtagtca tggttacagc
    11641 gaacaaagaa ggaaaacgct tgttcatggg attgacaaaa ggaaaattca gtcatccaga
    11701 tatcgacatg aaaacagctg aagacaaagg ggtagaattg caaaccgatt ccatcgaagg
    11761 ggaattcatt tctgatgaac gtggctatgt atacttaaca gccgtagaat cagaaggaat
    11821 gaccttacaa aaattcaagg acttggtaaa taacaaagag ggggagtagt taaccctgca
    11881 tctacaccaa cgacagatac agggacacca aaagaaccag aaccaaaaat tgatacacaa
    11941 ggttagccat ttttggctag ccttattttt tgtaaaaaca aggaggaaaa caaatgattg
    12001 aattgcaatt gaaacttgac ggaaagaaaa aaacattcaa acaacaagat atttccgcac
    12061 gtgcaatgcg tgagtgtatc aaattttacg agaaagcgga aaaagcagac ctaactgatt
    12121 tagaagcgat tgattcaatg attgcaatta cagcagatat tttccaagat ccagcagtta
    12181 catttgatgc tattttagac ggtttgactg cgagcgagtt agtaccggca ttagaaagtg
    12241 tttttgaaca aataaatgaa ctgggaaaca atgaaaaaaa gcagacggcg agcaaaaaga
    12301 aataagtttt tctgaagcta ggaaagcaat ggatcaaatc tacaaagatt taatcgaagc
    12361 aggttggacg atgagagatg tggacgaagc cgactatcat tatttgttac acctttttgg
    12421 agaagtggag agtggcgaag aatatgtaga tggtgctgat ttcatcaaac aatttttatc
    12481 ggctgaagac ttagtaaaac ttgaggaagg aggtaaataa tggcaggaaa aggacaaccg
    12541 gcaggaaata tcaagctagg gattagttta gatagcacta gttttggtaa cacgctggac
    12601 gaaatcaatg cgaaagtcaa acaagctgag tcgaatatgc gtgccaatct aaaggcttat
    12661 gattcagcag gacgttcata cgaagcactt agtcaaaaga cgaaagactt gtctacggtt
    12721 atggaagggc aaaacgccaa agtaagagaa ttaacaaagc gccgtgatga agcgattagc
    12781 aagtatggcg aggaatcgaa acaagttgct aaccttaaca cacagataaa caatgctacc
    12841 gcaaaatata atgcttacag tcgccagttg aacgacacaa aaaaagaatt ggtgtattcc
    12901 aaaacagccg tcaatgattt atctaatgaa atcaaagaaa atgaacgaca aatgaacgcc
    12961 gaagtaaaag cgttgaaagc cgctggtgat gaatctggtg cttttgaagc aaaacaaaaa
    13021 gggctagcca aacaaacgga attatccgag aaagctatcg aagaacagcg caaagttgtg
    13081 aaactgatgg ctgatgagtt tggcgattca gcaaatgaaa ccgaagatgc aaaaagggca
    13141 ttagaaaagt tagaacgaca aagccaaata tctagcaggc aattagaagc actcaaaagc
    13201 tccagcgatc aatcaggaaa agaaatagaa gattttggcg acaagtccac aaggtcagct
    13261 aggaaactgg acggactaaa agacaaatta agctcgctaa aaagcgcatt ttcgtttggt
    13321 gcagttgctg gattagcgca taacgctatt agcagtgtag taagtggcgt gcaaggtttg
    13381 gttggcgaag cagtaaacgc atcggattca ttgatgaagt tttccaaaac catggagttt
    13441 gctaactttg ggaagtcaca gatagaaagc tcgaaaaaag aaatgaaaga ctacgccgat
    13501 aagacggttt atggtttaga agaaattctg aacacaaccg cacaattggc atctaatggg
    13561 attcctaact atacagaact aaccaaggcg gcaggtaact tgaatgccgt tgcaggcggt
    13621 tctagtgata cattcaaatc cgttgccatg atgctaacgc agacggcagg agctgggaaa
    13681 ctaacaactg aaaactggaa tcaattagca gatgcgatac cgggtgcttc aggattgtta
    13741 caagacgcta tgttgaaaaa cggagcttat acaggaaact tccgtgatgc aatggcgcaa
    13801 ggtcaaatca cttccgacga gttcaaccaa gcaattgtac agttaggtat gaatgacgga
    13861 gcagttaagg cagccacttc tacagacaca ttgagcggtt cttgggagca gatgaaatcc
    13921 actgtaataa atgggctaca aagtattata gaaaaaatag gcgttgaaaa tatcactggt
    13981 ttcatcaaca gagtaacaaa agggattgaa aattctattc ctaaaattac tcaatttata
    14041 ggttggttga gagatattgg aacgtggatc gttgaaaata gagagccact aacatggatt
    14101 gtcggaatca taggcggaat tacattagca gtaaaagcat tgaacgtagc aagtatgttg
    14161 ctggcaatta ctggcggaac attggcagcc ccttttgtgg cgattggtgt agcattaggc
    14221 gcactagcag gtgctttggt gatagcttat acaaaatctg aaacatttag aaatatagtc
    14281 aatgcggctt ttacagctgt gaaaaacgta gttatgagcg ttatcaataa tttggtggaa
    14341 tactacaaaa tgttgtggag cgtgttgcag tggctttggg aaaaaataaa agaatgggct
    14401 tcatggattg gcaataaatt cattgaaatg aagaacagcg ttgtgaatac ggttcagaac
    14461 ctatggaatg gcgtgaaaaa cttcttcagc aatggcgttg gagatacgtg gaataaggta
    14521 gtcggttggg taaaaaacat tttcaacaaa gcaactgaat tgaagaacaa cgtttctgat
    14581 gtaatcggta acctgtggaa tggtatcaaa gacacattcc gtagaggtat cgatacggta
    14641 ttcaattggt tttcagaact accaacgaag atgaagaatg ccattattgg cggtaaaaac
    14701 gccattgttg atgcgttcaa aagtattttc aacgcagcac ttaaagcgat aggtaaacca
    14761 gttaacgcaa tcatccatgg agcttcatgg gtactagaaa aattgggcgc agaacctcta
    14821 caagaatggg atgtaccaca atacgctaca ggtacaccag caggtgggca cccaatcaat
    14881 ggtccaatga tggttaatga tggacgtgga gcagaaacag ttatcacacc agatggtaga
    14941 gccttcattc ctaaaggacg caatgttgtt ctaaacgcac caaaaggaac gcatgtcttg
    15001 acagcagaag agaccgcgca acttcaaggt tctaaagctc ctaagtatcg ttacaaaaaa
    15061 ggtactaact tctttggtaa catgtgggat agtgtgaaaa aggttgctgg taatgtaggt
    15121 cacacaatta aaaacgtagt aggtgacgtg tgggacttta tttcagaccc gggagcgtta
    15181 gctagaaaag tacttggggg gttagatgta ttaggtggat tgacaaagta tccattagaa
    15241 gtaggtaaag gcatcctatc taaagcaaca agtgcactga ctgaaaaaat cactgggttg
    15301 ttctcatctg gtaacttaga tacctccgta ggaacaaatg gcgtctataa atatttggct
    15361 gatgttgcta agtctgtgat gaagaaattc ccaggatttg tggcaactag tgggtataga
    15421 ccaggtgacc cttattcaca tggtaaacgt aatgccattg atattgcatt accaggcgtc
    15481 acaggaggct cacctagata cacagaagca gcaaactacg cttttgacaa attcgcttcc
    15541 aaaattggtt acgtaatcac taatgggaaa gttcgtgacc gttcaggaca atcaggtcaa
    15601 ccagcaactg gtgcatggga gccatggccc gatggagatc actatgatca tgtgcattta
    15661 aacggtgtga aagacccaca aaacactcaa atttcaggag atagcgtggg aggcagtggg
    15721 gtagaaagat ggcgcaatgt agcaattaga gcgttgaaaa tgaccggtca atacagtact
    15781 gcaaacttaa atgcattact aaatcaaatg cgtacagagt caaatggtaa tcctaatgca
    15841 gttaacaatt gggatattaa cgccaaaaat ggaacaccat caaaagggtt gctccaagtg
    15901 attgacccaa cattcagaca gtatgcaatg ccaggattca acagcaatat ttttgaccca
    15961 ctatctaaca tcttagcttc aatcagatac gcactatcaa gatatggctc actaacaaat
    16021 gcctatcgtg gagttggtta cgcaaacggt ggaattgtaa accaacatca aattgcggaa
    16081 atcgcagaag gaaacaagcc agaaattatt attccgttag ataaggctaa acgatcaaga
    16141 gcgatgcaat tattgtctat cgcaatggac aaactaggag tttctcctaa aacttatgga
    16201 aatgcgacaa ctgtttccag tgattacgaa atcctagaag ccattgaaaa acaggcggca
    16261 acaacgaatc agctgttatc gttattgctg gcatttttca aaggaaatag ccgaactgat
    16321 agagatttag ctttagatat tcagaagatc ttggttagga ggatgtagat gcgaactgta
    16381 ttactgagaa atagaataaa cgaagaaatt gatttgtcca cagaagacta ttttgctact
    16441 gggttgagta atatggggtt tgaagtaaaa aaagaacatg tgggacagtg ggggaatttc
    16501 agagaaagca gtgagagcgt tgaaatatct gaatttcagt catctgtaat catttctgtg
    16561 catgggtttc gagaaaaaga actgtacaat tcacttgtgc agttcctatc ggaaggtccg
    16621 tttgaactgg aatttgcttt tgacggcgaa acaatggtaa gaagatgcag tctaaaatct
    16681 ttaagtaaga ctgaaatcga tccgaaaaca tcgttactaa cagacacttt agaactatat
    16741 ttcacatcaa actggtattc cgtaaaacgc gaaaaattaa ttcagcgacc gaatgtagtg
    16801 aaaacacgtg gtaaggtttt tccgtataaa agatcttata tctacactca aaacttgtgg
    16861 gagaaaaaag gtgtattcaa attcaataat aattctgtat acctaacgaa tagcaaggaa
    16921 cggatgtccc cgctaaaaat tcgtgtgatt gggaagtgtt caaatccgta ttgggaagta
    16981 atccaaaact cacaaatcat cgcaacggat ggatacttca tagatatgac tgaaacacag
    17041 actctagaag tatcaagcct ttttgaagat acaacggcga ttttaaaaga tattgcaggg
    17101 gtagaatctt ctgtctacca acaacaggat tatacaaaaa ctaattttgt tcaggcccca
    17161 actggagaat tcagcattgt gtttcatgtt gggggggcag acgtagagat tgagttatac
    17221 gaggagcgtg atctgttttg attttagcag tcacgctttt tcatcgtgat ttaatgcttt
    17281 ataacgaaca tttattctct aacagttttg aatttggtgt ggacgagatc aacgaagagg
    17341 ctagtagctt tacgatggat aagtacgttc ctgtaaaaac aggcgatttt ctactagcaa
    17401 aatatattcc tagtggaaaa tttgcatatt ttggtgtaat tacgtcgcag gaagacgaaa
    17461 aaatcagttg taaaagctta cttagtttag ctgatagtga gataccgact gcacgtgtgt
    17521 caggagataa ctacgaagag catatccggc ggttgattga atactatttg ctgaatgatc
    17581 caacgaaaca actaaaagat attttagacg tcaaagcaga aagtgcgacc tctcattcgt
    17641 atcaagctac cgatacgaat aaacacaagt taagtgcgta tattctcaat ggtttcaaaa
    17701 aatacaacgt aaaatggtat ttcaagggaa ttcaaaacag aaaaatttat acaggcatac
    17761 gtgctgtaaa tgaatcaatt tacattaaag acaattcttc tgaatttagc gattgggatg
    17821 tgtttgttca ggcgccgggc gctggaaacg agaacaagct attaatcgtt gataaagcaa
    17881 tgaaagatat agagaaaccg ataatactgt caacatggta tttggacgaa gaaaacaatt
    17941 tgacacaaga tggatcgaaa gaaaatatca cgaaaccaac tgtcaatttg gttaatatct
    18001 acgatcaaac cgcagaagat aaagcatcct acgaagatgt ggcaaaatca gagttgaaag
    18061 gcaatacgta ttcgcatgaa atcaaagtga acgttgtaag aaatgcaaaa aatttgaatg
    18121 tcgaaacgat tgaaacaggg atgtttgcca cgatttctta taaaggaaag atatacaagt
    18181 cggttttgac agcttggcga atatcaagtg ataaggagtt tgtggaattg actttcggaa
    18241 acataagaag tcgttttatg gattattttg aagataatgg gggataaaaa atggttagca
    18301 atgtggatgg atatcaattt gaaaacgtga aagtaagcgc agaaaatgat gctagacttt
    18361 atcacgtttt atataatcgg aaaaatcagg ttattgatgg ttacgatcag tctatgaatt
    18421 tatcttcaag cggattaaca gtaaaagttg ctgcaggagc agcgattatt caaggtcgta
    18481 tggtcgttgt tcgacaagaa gaaagtataa cagttccagc aaactcaagt ggttatatag
    18541 cattaacagt ggatttgaca caagaagtta tacctggatc tattcttcca gaatcggaag
    18601 aatatgaatg gactaataat caagtcaagc tagaatttat aacaaaagtt ataaaaggta
    18661 atttaaacaa tggtgataag gtctataatt taccactatg ctcagttaat tctactggat
    18721 caactgtttc aatctcaaag atatcggata gttacgagct gactctctct aaaggagaaa
    18781 ttttgtggag ggggactgcg ttaatgcatg atactcaaac tgtccaacct tcaaaaaaaa
    18841 tttggcagac agtttcaggt tttttgttag tatggctccc atacgaaaac gggcaagcaa
    18901 ttgaggatag atatgtaact acgccttttt ataaggagcg tgtaactttt actaatgttt
    18961 taggagaaat tgtttcagga tttgatgact atcacaaaaa gtggtttagt aaacgaataa
    19021 actataattc gaatactaat atatttacag gtgcagcaag caatgcaagt ggagataacg
    19081 caaatatggt gcttaggtat atagtttctt tttagttagg aggtatggaa gagtggcaaa
    19141 tttagaaatt aaattatctg caaataagag acagccttat ctacgtcacc gtgttgttgg
    19201 tagagttggt gatggggggc tcacaacaat caatgtacaa cttcttgagg aagatgaaat
    19261 tacacctttt gtaattaatc taaacggtac tttgaaattt gtgggcgaag tttcaaacgg
    19321 taactatacc gagggagaac cagaaataat cgattcgact aatgggttaa ttagttacac
    19381 gttcactaag tcaaatttca gcacgagtca tcaattcaaa caagcatatt ttgaatatgt
    19441 agatcctaac ggcaaaaaag taacttttca gaacttcatc atagacgtgt tagaacgagt
    19501 ggatattaat tcggagcaag cgaaatacta tatttcttca ttggaaaaat tacagagtga
    19561 aatgcaaacc actttcaatc agttcattag tgataaacag gtccaatacg atcaaatcta
    19621 ctcgaaatat aacgaattag taagattgat aaatgaatca gataagcaag taaatgatcg
    19681 tattgatcaa accaatcagc aaatcggcga tctcggcaag ctgaaaaaga tgtacagtaa
    19741 cagcatcgac tttgggggct atgattattc ggggaatccg aatttgttaa gtaagctatc
    19801 atacgactta attgaaaatc aaaatacttc agctggaaca ctttctaagg gtgaaaactc
    19861 gtttaaatat aataagatat cagctgaagt ggaaggtgga gtagagttat attataaacg
    19921 aagaggtata gctaactggt taccctctaa taaaacgctt gtaatgactg ttaagcttag
    19981 agctggagcg gactatagtc cagttgacgg aaaacttata ctgattagat ataggtatgt
    20041 tgacagtgga actggcaaga ttgttttaga cttacctatt aacagtaatt cgataactca
    20101 ggaatggaaa gagtttagta ttactggaac tactccaaca tttagcccac aagcatacca
    20161 tccttggata caatttaggg ctcaagatgg ggtacttggg gaaatagaaa tgagctatga
    20221 catcaaaatc gaagaaggct ctacagctac accatatcag cctaacttac ttgatgactc
    20281 ttactggcta ggtaaaacac cgttgggtga gaatttgata acgaatgcca agttcccaat
    20341 tataacacga agcaatccta tttctggttt tgatatttca gaagaattaa taataggtga
    20401 aacttatacg gtatctttaa aagggactaa gccagctact aaagaattcc acttatacta
    20461 cggagaagca aattaccagc agttgcagta tcaggcaact ttacttcctg tggaaggatt
    20521 aacagatgtg tggagtgcca cttttacagc cagaaatact agtgaaacga caaatttact
    20581 tagagtagcg ttatggcaaa aacctaattc tgcaacatat gatactgttc aaattgactg
    20641 gctcaagatt gaaaaaggcg acacacgcac accaaatatc agtcagttta aatactttgg
    20701 tgaaggcttg aaagacagta acaatccgaa cgactacagc tgggatgtca caccagagta
    20761 tactgaaaaa agcttgaata atacggttag tctgactgaa ccgcaatcca tcgaaggatt
    20821 gaaaaatttc gaagatggta ttcaatcaaa aggaaaatct gtattgacat cagacgacaa
    20881 caaatatgaa gtcgtaacct taacagttac aaacgggaat accggatcag caaagcttta
    20941 tcgtgaagga aaaaccgtca ctatttattt ttttgcgttg aatgggaaaa gcagtggtgg
    21001 aaatgattca acgatactaa caattccaga aggctatcgg ccaccaatta gttttgagca
    21061 actggttggc tcgatagacc gttctacttt gaacagtgct cagttatcta ttggtgcaga
    21121 tggagccatt aaatggcgaa gaaactcaag ttatggatcg gattatacct ttgcaattac
    21181 ttacacgatt tagaaaggcg tgaatcgata tgaaggcagc atatagacca attgaacctt
    21241 acggattcga gcaaatcatt gtgaatgatg aagaacattt accggaagaa tgcacagaag
    21301 tcgaaccacc gattccaaat tggaaaccga aattcaatta ctgggaggga aataaatgaa
    21361 aaacatttgg aaatatggcc gtactggcgg agagtatgca ggaaaagtat tggacgacat
    21421 gcttgtatcc gttccttaca cagatcagcc accgctcgaa ggggttcgtt cagatggcga
    21481 accgctaacg attgctgatc agatgtttga tcctaaattg aaccaatgga ttattttagc
    21541 gaacgcgtta gatcacaacg atttaaacaa tctcaaagcg atgtacgagg ctcttgaaca
    21601 tgaaaacggc aacctaaaac agctaaatgc caaactcatg ctaagcgatg tagcgattaa
    21661 acaggaaaat actgcattga aagaaaaagc tgacagttta gcacaaatca attcaaaaat
    21721 gatgcttgct tcgttacaaa atagcaaaga catttcagaa attaaagagc aactaaatcc
    21781 agcttcaaag ggaggtgagt agtatgttta gttttagcga tgtgaaaatg atgtatgatt
    21841 ggggctgttt tactgacgat caagttcgtc tattcgttcc actatgtatc acagatgaag
    21901 aagcagatag aattattagc aaagaagaga gcgcatctta agtgatgcgt ttttttgttg
    21961 gaaagttggt ggaacatgaa agaagaagcg ctccaagacg ttgtggagag attagtaaga
    22021 attgaaacaa aattagacaa ctacgaatca cttagagaaa aagctgatag tgcaaaagat
    22081 ttggcagaca aagcctactc agtagcgcta aacaacgcag aagatatcaa agaaatgaaa
    22141 gccaataata aatggtcgtg gggttacatg atcggtttag gcattacgat cattggctat
    22201 ttcttgacta aattgtaaag gagttaagaa gaaatgattt tacccgataa gtattatcaa
    22261 gtcattaaat ggacggtttt aacagtatta ccagctgcat ctgttttagt agccacgtta
    22321 ggaaaagcgt atggatggaa tggaacagat atgacagtac tcactatcaa tgcagtagca
    22381 acatttttag gcgttatcac tggtgtgtct gcatataact taaaaaaata ggaggaaaaa
    22441 aatgaagaag aaaatcattt tatcattgag cctaataatg gctctttttt tattgccaat
    22501 taacgggttc gcctatacga tcaacaatga atttaattta ggtgtaaatg aaggtagctc
    22561 acaagtagca aataaccaat acatcctatt gcatgaaaca gcaaacgaaa ctgcgacagg
    22621 acgaaatgaa gcgcagtaca tgaaacgttc ttggtacaat gcctatacag cgtatattgt
    22681 tggcgatggt gggatcgttt accaagttgg acaacctggt tatgtacaat atggcgctgg
    22741 ttcatatgct aatgcaaata gtcctgtgca aatcgaactg caacatacgc acgataaagt
    22801 aacgtttgaa aagaactaca aggcatatgt tgaattagcg agagattccg ctaataaata
    22861 cggtattcca cttacattag acacaccgta taatcaacca ggaatcaaat cacatttatg
    22921 ggtaactcaa aacatttggg gcgatcacac tgatccgtat ggatacttat cagaaatggg
    22981 tgtaagtaag gaaaaactgg cttacgattt agctcatgga tttacagacg aaaacccaac
    23041 gacttctgag aacaagcctg tcattgatcc aacccgagca ggtgcagcaa atccaacttt
    23101 atcagatgga acgaaccatt ctcacattga tcagtttgga gaaatcgaaa atgcgaactt
    23161 acacgtcgct ggatggcaca tcgctaacta taaatacgag tatattttca ttatggacta
    23221 caatactggg aaagaattag ctcgagtaag agctgatgga atttatagac cggatgtaaa
    23281 tcaagcttat aatacttctg gaaacgttgg ctatcatgta tcttttaaca tgcgcaattt
    23341 ccctaataag aaagtatacg tcatgatgcg ggcaacgaat gatccagaag gaaacactaa
    23401 aggcggtgcg caagatttcc atgacaaacg ttggtattta aatattccta aacgataaaa
    23461 atagctcctc gttgaggagc agtacataga attgaaaatt aacgttaaat caaaaaaata
    23521 tttacaagat gtttatttaa gtagataata aaagaaaaaa gcaggtgaaa ttgtgacagc
    23581 agaaattggt attatgaata aaagtggaat cgtgttagct tcagattcag catctacgat
    23641 tggagatagc aaagtatata atactgcaaa aaaattattt actttggatt ccatgcattc
    23701 tgtaggtatc atgatatatg gtaatgctga atttaatggt attccttggg aaataatcat
    23761 tactcaatat aagaaaagca ttggtagttc tgtttttaat actttggaag aatacgcgga
    23821 taattttata ga
//
LOCUS       DAFGFZ010000038        23611 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000038 DAFGFZ010000000
VERSION     DAFGFZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.038"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            26..670
                     /locus_tag="LR726_001279"
     CDS             26..670
                     /locus_tag="LR726_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL6216381.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(726..1229)
                     /locus_tag="LR726_001280"
     CDS             complement(726..1229)
                     /locus_tag="LR726_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL6216382.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(1242..1288)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1826..2791
                     /locus_tag="LR726_001281"
     CDS             1826..2791
                     /locus_tag="LR726_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL6216383.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(2890..4062)
                     /locus_tag="LR726_001282"
     CDS             complement(2890..4062)
                     /locus_tag="LR726_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216384.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(4049..5194)
                     /locus_tag="LR726_001283"
     CDS             complement(4049..5194)
                     /locus_tag="LR726_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216385.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(5208..6132)
                     /locus_tag="LR726_001284"
                     /pseudo
     CDS             complement(5208..6132)
                     /locus_tag="LR726_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6249..7328
                     /locus_tag="LR726_001285"
     CDS             6249..7328
                     /locus_tag="LR726_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL6216386.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            7360..7953
                     /locus_tag="LR726_001286"
     CDS             7360..7953
                     /locus_tag="LR726_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6216387.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(8063..8290)
                     /locus_tag="LR726_001287"
     CDS             complement(8063..8290)
                     /locus_tag="LR726_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL6216388.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(8393..10981)
                     /locus_tag="LR726_001288"
     CDS             complement(8393..10981)
                     /locus_tag="LR726_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL6216389.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(11001..11660)
                     /locus_tag="LR726_001289"
     CDS             complement(11001..11660)
                     /locus_tag="LR726_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL6216390.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(11709..12212)
                     /gene="trmL"
                     /locus_tag="LR726_001290"
     CDS             complement(11709..12212)
                     /gene="trmL"
                     /locus_tag="LR726_001290"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL6216391.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(12433..13299)
                     /locus_tag="LR726_001291"
     CDS             complement(12433..13299)
                     /locus_tag="LR726_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL6216392.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            13408..14049
                     /locus_tag="LR726_001292"
     CDS             13408..14049
                     /locus_tag="LR726_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL6216393.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(14111..15472)
                     /gene="mgtE"
                     /locus_tag="LR726_001293"
     CDS             complement(14111..15472)
                     /gene="mgtE"
                     /locus_tag="LR726_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL6216394.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(15490..16389)
                     /locus_tag="LR726_001294"
     CDS             complement(15490..16389)
                     /locus_tag="LR726_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL6216395.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(16391..17188)
                     /locus_tag="LR726_001295"
     CDS             complement(16391..17188)
                     /locus_tag="LR726_001295"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL6216396.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(17166..17870)
                     /locus_tag="LR726_001296"
     CDS             complement(17166..17870)
                     /locus_tag="LR726_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL6216397.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            18001..18576
                     /locus_tag="LR726_001297"
     CDS             18001..18576
                     /locus_tag="LR726_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL6216398.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            18694..19371
                     /locus_tag="LR726_001298"
     CDS             18694..19371
                     /locus_tag="LR726_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL6216399.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(19450..21258)
                     /gene="pepF"
                     /locus_tag="LR726_001299"
     CDS             complement(19450..21258)
                     /gene="pepF"
                     /locus_tag="LR726_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL6216400.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(21434..22432)
                     /locus_tag="LR726_001300"
     CDS             complement(21434..22432)
                     /locus_tag="LR726_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL6216401.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(22658..23311)
                     /locus_tag="LR726_001301"
     CDS             complement(22658..23311)
                     /locus_tag="LR726_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL6216402.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         7052 a         5006 c         4112 g         7441 t
ORIGIN      
        1 aaatgagaca attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga
       61 tctgacaacc gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc
      121 cggataccgt tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta
      181 caaaatgcat atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca
      241 tcttttgaaa aacgctacaa agttcattta cctggatact tctttcttca aagtattctt
      301 tacgatcaaa gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat
      361 tttgacacat agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag
      421 ctatgtactg acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat
      481 catttcaaaa cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata
      541 cattcgacgt acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat
      601 cgtcaaccac ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca
      661 agaagcataa tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt
      721 tataattaga cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct
      781 gaaacagcca tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct
      841 tggccacctg ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc
      901 gtgttcaata ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg
      961 cctaaagaaa gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag
     1021 aaggcattat agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca
     1081 actaaatgat tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca
     1141 actaaaatag ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat
     1201 aaccgttgtc tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa
     1261 cgcgggatgg ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc
     1321 atttatttca atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt
     1381 ttgctaaaaa tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt
     1441 aaagcagacg aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa
     1501 aggatgtggg aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc
     1561 taagtggaca tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc
     1621 taggcttttt tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata
     1681 ataaccaaga attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa
     1741 aaatagaaaa agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg
     1801 ttttattgaa tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc
     1861 ggcaggcagc ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa
     1921 gcatattgac tatacacttc attatagtga atacaaagga cacgatgctg agattgctga
     1981 aactttggca aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac
     2041 tttccccctt ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt
     2101 ctatcagatg gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt
     2161 tgctcgaggg gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc
     2221 tcaatcacca gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg
     2281 aatagctgtt aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca
     2341 ttcttccaca aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc
     2401 tattctccgt gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa
     2461 acaattaagt ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg
     2521 aggaatcgcg attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt
     2581 cgaacgaatc aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca
     2641 attgaaatcg aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac
     2701 accgcaacat ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat
     2761 aaccacaact aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct
     2821 ggaacaccgt ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt
     2881 actatgcagt taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa
     2941 aataaattgg atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca
     3001 aaaagattct gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg
     3061 aacaaaccaa tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc
     3121 tgctggtaca aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc
     3181 accgctggtt tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg
     3241 caataaaaac aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg
     3301 gatcactaac agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat
     3361 caaactaaag gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg
     3421 tttactgatc tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga
     3481 tagaaacaac ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg
     3541 ctgactatat cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg
     3601 ttccatagct gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa
     3661 ggtgttcact tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt
     3721 gatcgtcggc atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat
     3781 aatgtttaca cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa
     3841 tgtaacgact gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc
     3901 ttcttcacta atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt
     3961 ttgcgcaaaa aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt
     4021 atttgcttta agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca
     4081 tccaagcatt tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat
     4141 aaaaactagc caaatcagtg tagaaataaa gtttatacaa actttcgctg atcaagttga
     4201 cgagatttaa ttgaccaaca atcggaatat gaagatcaat ccaatacttc acttgttccg
     4261 accccatcat accagcaaaa aagctcatcg caatcgtcac tgaaatcaag atgctatcct
     4321 tttgcaccag attcattttt ggtatcaaat taccaaccaa tgtacctaac aaaatcgcac
     4381 acaacgaccc cagagcggta acaatcatca accattgcca acgttcacca aagtctactt
     4441 gatagataaa acgaaagaca gctaacataa tataaatctc tgtcaaaaca atcgtaaagc
     4501 ttgctgccaa attagcgatt gaaactaacg attgctttct aggaatcaag gacaagcgaa
     4561 taccatttga cgattgattt gcttgctgat cctgtacatt gcggaccccc cacatcgtcc
     4621 cgtagagaat cgccattcca atcagagtga agaaataaaa acttttgaaa ctcatattac
     4681 gacctgcatg aacttcttga aaagatactt cttgttcaaa aacagtcatg atgggcgcga
     4741 gatcaacgtt ctccacttct gccactttac tcgaaattat accaatacgc tgtaaaaatt
     4801 gatccatgac gtttttcatc aacgtctgac tcattcctgc ttgacccaca tggagcgaaa
     4861 cagatgaacc atcaatgaga taaaaaccag ccaaatcatc tgcatttagc ttttcattcg
     4921 cttctacttc cgatagttcc tgtacttcaa ataatggttt atcattgact tcgacttgtt
     4981 ctaagactgt ggtaaaattt tccgaaagcg ctgtttgatc cgctttgatt acaattccag
     5041 tattaatcgt ttcgagattg ccaccttgat caatctcgcc aaaggccgcc ataaacatca
     5101 atcctaaaag aattggaaag gccaatgtcc aaaacaacat ggatttattt cgcagcagta
     5161 tttttgtgcg atatttataa aaatgccaaa acattatttc ccctcctcta gtctctcaaa
     5221 tccttgccag taatctctaa aaagacatca ttcaaggacg cttctttggt tgctattttt
     5281 ccgtaactga tcttttcttc ttgtaagatt gtaagaatcg gcaacaatac tgtatttagt
     5341 tcagtagttg ttacttggag ctgacctttt ttataagcga ttccatcaga agctattgtt
     5401 cgctgacaac acgttttctg ggagtaatgg gatgtcgatc gtcaaacgat ccgccgtccg
     5461 taccatatct tttagagatt ctttggttcc tttagcaatc acctgtcctt gatccatgat
     5521 cacgatctgg tcgcaaagaa tttctacttc ttccatgtaa tgtgttgtat agacaatggt
     5581 tgctccttgt cgattcagct ccttgatact ttctaaaatt ttattccgac tttgggggtc
     5641 tacagctact gtcggctcat ctaaaaaaat taaccgcggc ttgtgggcaa tcccacatgc
     5701 gatgttcaac cgacgtttca agccaccact aagttctcct ggacgaaacg aaacatattt
     5761 ttctaatccc actaattgaa tcacctcttc aatcaaggct ttccttttct ctttttcgga
     5821 aacatataaa ccgcaaaaat aatcgatatt ttcttgcaca cttagttcgt caaagaccgc
     5881 aacgtcttga ggaacgaccc caatcattgc cttgacagca taattttcag gagtcattgt
     5941 tgtcccgaac aatttaatgg ttcctttgtc ataatttaac aaagaaagca tacagttgat
     6001 ggcagtcgat ttcccactgc cgtttggtcc taacagacct aagacttctc cttcttgtac
     6061 ttgcagatca aaatgattca atgctacaaa atctccatag cgtttgacta atttatcaat
     6121 ttcgataatc atcatgaaaa actccttcca aatttcttgt tgatctgact ataataaagt
     6181 gcaagaaaag aaactagtga cgattggcta aaccacacat gacatttgtc acaatgaagg
     6241 agtctgctat gcataaatct cggctactca acgaaactag ctttctactg gttatctcat
     6301 tgcttttgct ggtaagtcaa ggattcgttg ctatgcaagt tatctattta ttgatcgccg
     6361 ttttattcac tggtgtcagt tatttactgc ctacaaagcc aaattggatt ttccaagtta
     6421 ttctatttct tttatgtgcc tttttacctg atcttgtcct gtttctgcca gcgacgatgc
     6481 gagtgatcgc ccctgaagaa agccaccttg attacttgtt tttgttaggt tttttgttga
     6541 tttcgacggt tggtaatcat ccccccatca aggtcgcctt tgtcgtaatt ttgctgtttt
     6601 tttcctttta cttacgaaaa aaagatcaac gaacgaccga tcttgagaaa aagtacattg
     6661 tctccaaaga tactcattgg gaaaaacaac aagagttatc ggaacaaaat aaagcattgg
     6721 ccctgtcaca agaaacacaa ttagccttgc aaattgtgga agaacggaac cgaattgccc
     6781 gagacatcca tgacaatgtc ggtcatctgc tgtcaagtgc gattttacaa attggtgcgc
     6841 ttgaaatgat caatcaagaa ccaaaactaa aagaaccatt agaaaattta agtgccaccg
     6901 ttcacactgg gatggatcgg attcgtcaaa gtgtccatga cctccatgag acatcgatct
     6961 cttttcaaca ctccttagag tttctgatcg ctgattttcc ttcttctgtt acgattgaag
     7021 gaaacttgtt tgaaggattg aatgaaacgc agcaaaactg cttcttaatg gtcatcaaag
     7081 aagccttaac caacagtatc aaacatagtc atgccaaaac cgttgtttta cattttcgaa
     7141 cgttgccggc cttttatcgc ttgcaaatca cgaatgatgg caccagccca caaaaaaatt
     7201 ctgctcccgg tattggtttg aaaacgatgc gtcaacgtgt tcaagaacaa aagggacaag
     7261 tccatatttt ccaagaaaac cagcaatttc agttaacgat cattttacca agggagggac
     7321 accaatgata acagtagtca ttattgagat gatccatttg tgacgaaatc gttacaaacc
     7381 attttagaaa gcacgaaaga gatccgtgtg ttaggcattg gtcattgtgc caaggatgcg
     7441 ctagtcttat acgaaaccca tcgtcccgat gttttgctga cggacatccg catgcctaaa
     7501 caaacgggca tcgatgctgc caaagaaatt ttggctaaat ttcccaaagc catccttttg
     7561 cttttgacga cattcaaaga tgaagaatac atccgcgaag cattttccat cggtgtcaaa
     7621 gggtatttaa tcaaacagaa cttgcaagcg attattcctt ctgtcaaatc cgcttataat
     7681 ggacaggccg tatttggtaa tgaaatcatc gagactttca ctcaaatgat gaagaataaa
     7741 ccgacgattg atagtcatgc ttatgcttct tttagtgaac gtgagcttgc aatcatcaaa
     7801 gaagtagctg ccgggaaaaa caataaagaa attgcagatg ccttgtactt gagtgacggc
     7861 actgttcgca attacatcag ccaattacta gaaaagctag atttacggga tcgcacgcaa
     7921 ttggctatct atttttatca gcatttgcaa taagtcttat ctaacacaaa aaaacctgaa
     7981 tgatgctcct tcgcatcatt caggtttttt tcacatcttc tattcaattg tcccgcaaac
     8041 catttcctca cgattgaaag aatcaaggat tcgcagggct gccaggattc gttggattgt
     8101 tcggcgtgtt aggcggaaaa ataggcgcct tgtctaacgt tttttctagt tttgattctt
     8161 cacgatcgtc tcgttctaca tcaggacttt cattcaattt gaattctttt tccatctcat
     8221 ctaacttttt aaagtcattg ccagattctt ttgctaattt cgctaattta tcttcggttt
     8281 ctttagccat aaagtaacgc cccccttttg atttcagctt agcatatcac actgaaaaaa
     8341 caaaagaaaa cgctaaccaa cgatcatttt tagctatttc tcatctttaa aatcaagcat
     8401 tttttgccgg tataaaatcg tagggagaac tgttttccat accaatcatc ggatctacta
     8461 actggttatc gatcacttct gctgtaagaa cgaaatcttt agaaacagca ggttctacct
     8521 cttctttctt ttcttctcca acaaatgaag aaaacagatc tggttccact gtttccatta
     8581 cttggttttt cgtttcattc gtcccactgt catcttctac aggtagcttt tcttttacaa
     8641 cccgtggctc ttttgtatag ttcccatctt caaatggatc atttccgacc aaagaatctt
     8701 cataagctgc tagtttggtc cggagagtga tattcatttt atcattatcc atgatccttg
     8761 atttcaacgt agtcatccgg cttgctgatt cattcgctac acaagtgaca taggcttgct
     8821 cttcccctaa caaaatcaac agttccttac tgcgagtcac tgccgtgtag agcaaattac
     8881 gctgaagcat ccgctgatat tgatggacca tgggcaaaat aaccatcttg aattcactgc
     8941 cttgtgattt atggattgag caacaataag aaagagtgat tttattccat tcgtttcttt
     9001 tataagtgac ctcatttgca tcaaactgga taactaattc atccacttta tcttcagaat
     9061 cttttgccaa gatgataccc gtgatttctc ccatatctcc attgaataca ttcaattcgg
     9121 gagtattgac taattggagg actttgtctc ctatccgata aacggtgtcg ttccaatgta
     9181 cttccttttt tccaggtttc cctggattga atatctcctg catcatttta tttaacgcat
     9241 cgatccctgc aggtccccga tacattggtg ctaatacttg gatatcttgt gcacggaatc
     9301 ccttattttt cgctttttcc acaattttag agatcaaggg ttcaatttga taagcatcac
     9361 ttcggaaata ggaacgatct ttttgattct ttgtgaaatc ttttggcaga cgtccttctt
     9421 tgatctcatg ggcaagagga ataatactag aaccatcacc ttgccgataa atctgattga
     9481 gttccatttt gggaatttgt tcgatttcca aaagatcatg gagtacttga ccaggaccga
     9541 cagagggaag ctgatctttg tctcctacta agataacttg catattcgtc ggaatcgctt
     9601 tgaataatgt gttagccagc catgtatcga ccatagacat ctcatcaaca atcaaaagtc
     9661 cgccttctaa ttctttcgta ctgactgaag gtgttttttc ccgtccattc agccccagaa
     9721 gacgatggat cgtactggca ggcagacctg tcgtttcgtt cattcgttta gctgctcggc
     9781 cagttggtgc tgcaagtaga ataggaaaaa tttcttgggt atattttgaa ggatccaatt
     9841 ccagtccgtt caactctgca aaaagattga cgattccgtt gatcacagta gttttccccg
     9901 tacctggtcc tcctgttaaa ataaatagag gggaacggat tgcttcagcg atagcttgtt
     9961 cttgcgaagg tccatactgt atgccgaata ttttttcgat acgccgaata ttcttctgca
    10021 cttctttttc aggataatgg atttcttttt tacgactgag aaggcgctgg atcgaattac
    10081 cgatccccca ttcagaaaag tacaaactgt tttcgtaaag attagtctct tcttgttgta
    10141 tctttccttc ttcaacaagt tggataatta cagtagcaac ctgatcaggc tggatttcaa
    10201 ttgggcgact tgtttctaaa atacggatcg tttgctgaag caatgtctcc gccggtacgt
    10261 atgtatctcc tgtttgaact gcatgctgaa aaatctgatg caagatagct gctcgaattc
    10321 ttctcggtga atctgctgca ataccgattt gttcggctag attatctgca cgtttgaacc
    10381 cgattccttc tatgtcttcg actagctggt acggattttc ttcgatgata tccaaagact
    10441 cgttcttata ggcctggtag atactgaaag ctaactgact accgaacccg taacgattca
    10501 gtcccatgat cacttgatcc atcccgtggt ttagccgaat cgtctcagca agcatttctc
    10561 tttttggctt cgtcaaaccg gagatttgtt ttaataccga aggatcagca atgatttttt
    10621 cgatagcatc ttctcccagc aattctacaa ttttttctgc tgttttcttc ccgattcctg
    10681 ggaatttttc gctggaaaga tacgctacga tcccatttgc agttgttggc ttttcttgct
    10741 catatctttc gagtttaagc tgccttccgt atttaggatg atccactaat tctccataaa
    10801 aacgataagt ctcattttct tgaatatcgc cgaaacttcc ggtgactaca atctcttttt
    10861 cacggtaatc agctgtggtt tcactgatgt gtaccaaaag cactttgtaa aaattactag
    10921 gattttgaaa aaaaatcgcg gcaactgtac caacgaaata aggcatttct tctggctgca
    10981 tcggaagccg cctccttctt ctatagtaaa ggttctggtg ctgtttcagt tcctaaaaaa
    11041 tctgtttggt tccatacttt cagttcatat ggcaataatc tgcaaggctc ttcagtctct
    11101 aaaatcgtca aactactatt gaacaatcca cccatttcac gcaactcact tagctctttt
    11161 cctgtcatcc attgaatggc tgcagttaaa gaagctccat gtcctacgaa taaaagtggg
    11221 ccatctttat gttgggcaac cgcatctgct acaacagttt cgatacgtgt gattgccgat
    11281 tcaatcgttt ctccgtgaaa aggagaagga tcatacagat ctaaatgatt acgcaaattc
    11341 ggcagattat ccggatattt ctggtacatt tcatcgatcg actgaccttc taaagtaccc
    11401 agccctagtt ctcttagctt atctgtataa aaaatctcta ccggacgagc aagctctttg
    11461 ttgatacctt ctgctgtatc acgtgctcgc tttgctgtac tggaataaat tgctttaaaa
    11521 ggaacatatt ttaccttttt cccaaatgct ttgatttctt cgtaactttg tggcaaaagc
    11581 ggagaatcgc cattgctccc ttgaaaacgt ctttcctgat tccattgtgt cttcccatgt
    11641 cgggtaaaat atagttccat acgcagcaaa ctcctgtctt tcttttattt tactctataa
    11701 taaactgtct aatccagctt gtcattttca tagtgatgcg tcaattctaa attagggaat
    11761 ccttgctggc gaagtgcttc atacactatc attgcagctg tattcgataa attcaatgag
    11821 cggacatgct catcattcat agggatgcgt aaacactttt cttcgttttc acgcataaat
    11881 gtttctggca aaccggttgt ttcttttcca aataaaaaga agtgctcttc tccatcagca
    11941 aaattttcgt cactatatac tttatgggca aattttgaga tcaaatgtag tttacgatta
    12001 cctaaaaatg ttaaaaaagc ttctaaatta ctgtggtaag taatatttac atcattccaa
    12061 taatccaacc ctgcccgttt tagatgtttg tcatcagttg aaaaacctag tggctcgatt
    12121 aaatgaagcg gagtattcgt ggctgcacac gtacgggcaa tatttccagt gtttgcaggg
    12181 attaatggtt caaataaaac gatatgattc atcaggatca ttcctctcat atttatgttc
    12241 gtttctattt tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac tcggtgtcag
    12301 tcactgcgag tcgttacgtt agtgaagctc ttctcactaa cttttatcag aacaggaacc
    12361 tgataaaaac caatgaaaaa acaacttgtt gctaatataa caccctcaac ttttttttgc
    12421 aacctctact cattaaaaac tgctagaaaa acgttttctc ttagttcctt ttaataattc
    12481 tatgtcaatt gtaatcttct tcaaaggatt cttatataag tctgctttga ttcgctcttc
    12541 tacctgccat tctaaaggtg acatggccaa aagatctttt tgattttcct ctggtatctc
    12601 aaattcatag gttacacgct gacgttcagt taatttcatt gcttcagcaa acttggctac
    12661 tacacgttca ttggaataac tctgtttttc aggttgattc ggataaaaag cctgacgtag
    12721 ttcctttaaa taattttctt gtggcaccac cttcaacact tctccatctt ctgagagtac
    12781 acgttggaat tcttggtaat gagaaggcga aaagatattt aaaaccgcgt tgaatgtttg
    12841 atcagcaaac ggcaaattgg tcaaatcagc tacacaccaa aatgcttcgg cagattgttc
    12901 tgtagctgcg tagactcctt cttttgaaat atcaaatcca acagcatttt gaatcttttt
    12961 caattttgaa agttcattca agaagctacc ttctccgcag ccaacatcta atagcgagac
    13021 ttcttcaggt aaatgctgcg caatagtctc aataacgggc tgatacatgc cagattggat
    13081 cattctacgc cttggaacga acattccttg atcgtattct gttttaaccg catgggataa
    13141 aaaataaagt gttccttttt tggaaaggtc aaaacggtga ttatttttgc atatcaagcc
    13201 attatcagac ttttttactt cttcttggca aaccggacag cggaacagct gatgattttc
    13261 tgccaaaaac tggcgtcctc ggtcaatttt ttttaacata gttccccctc atttctgttt
    13321 tatcttaccg ttagatgctc actttggcaa ctgctaaaaa tatgatacaa tgcctagaag
    13381 aaagtgaaag ataaaggaga agattagatg atcaaagaat tttgcgctga aaactacaca
    13441 tctattcctc tagcaattgc tagaggagcc gatcggatcg aattgtgtga caacttagcg
    13501 gtcggtggca caaccccgag tactggtgtg atcgaagaaa cgttaagtta tgctggggaa
    13561 aaagatatcc ccgtaatgac gattattcgt cctagagggg gcgacttcgt ctataacgat
    13621 atcgaattga aaatcatgca tactgatttg attgaagcaa agaaattagg gacggatggc
    13681 gtagtatttg gttgtcttac tccttcaggc tggctagacg aagaggcctt agaactattg
    13741 atcgaaacag cagaagggtt gcagatcacc ttccatatgg cttttgacag tattcctgaa
    13801 gatcggcaat tcgaagcaat cgattggctg gtagaacatg gtgttgaccg tatcttgact
    13861 catggcggtc ctgctgacaa aactattgta gaaaatttcc cacggcttaa agaattgatt
    13921 gcctatacag atggccgctt aatcattttg cctggtggtg gtgtcactgc agagaatgca
    13981 gcacatgtag ctaaagaatt gaacgtaagc gaagtacacg gaaccaaaat cgtggatctt
    14041 cagttataat cttataaaca aaaaaacatt tgcaacaggc ataatgctta ttgcaaatgt
    14101 ttttgttttt ttacataaaa tagcccataa ataaagaagc aatattgaaa taaatcagta
    14161 cactcgtcaa atcgctcaaa gtcgtgataa agggaccgct tgctactgct ggatcaaatc
    14221 ctagacgatc cattagcatc gggattaaac tacctgcaag attcgccact gtgattgcaa
    14281 atagcatagc catccctatg acaaacccca agataaaatt atgtttccaa aaacctacga
    14341 ttagaaaaat cgtgatcccc gtaacagctc ctgtgacaac gccggtcaag acttcagcca
    14401 aaacaagtcg aacaaatcca tgatctttct catcagaaat cgcaagtcga cgaacagcca
    14461 cagctagcga ctgggttcca gcattaccgg cagttcctgt aatcagcgaa atgaagactg
    14521 ctaaaatact cgcttcactt accaagtctt catagtgact gattaaagaa gcggtcgaca
    14581 tacctaaaaa caacagagtg atcagccaag gcaaacgtcg agacgctgcc tttactggat
    14641 tttcattaat ttcttccaca ttgaccccgg caagtcctga ataatcgcta gcagcttcat
    14701 catcaataac gtcgatgata tcatcgactg tgacgattcc taacaaatga tcatcataat
    14761 cggtaacagg aagagctaag aagtcataat ctctaaatgt ttgtgccaca tcttcttggt
    14821 cgtcgcctac atgaactgaa agcactcgtt cgctcatcag atcagaaatc attgcgtcgt
    14881 catcattcac gattaggtcg cgcagagaaa taacccctac tagctgacct tcttgattaa
    14941 cgacatagat gtaatagatt gtttcagcta catctgcttc gttcttcagc acgtacatcg
    15001 cagagcgaac agtttgattg gctacgatcg aaacaaattc agttgtcatg atcgcgccag
    15061 ccgtttcatc ctcgtaatgg agaagttcct tgatttcact ggcatcgtct gccgacatca
    15121 aacttaaata tttagcaatt tgctttttat ccagcgtatt aagcaagtca acagcattat
    15181 ctgtatacat ctcagaaagc atatcagctg catagctagg acgcatttca tctaggtatt
    15241 ctttcatatg ttcgttgtct tcttcaatca catcgaacat atccgctaat tctttaggcg
    15301 acagatagga atagatcagc tgcctgttct cttctgtaag agattggtag aattgcccct
    15361 gctcatagat atgcatctct aaaaaactgt ttctgaatgc ctgaatatta ttttccctta
    15421 aatcttgagt caactgggca aatctttcct ctaattcttg attttcatcc aaaccaatct
    15481 cacctcgcct tattcttcaa accgtctcac aactgcctcc atgtctgcgg ctaaaggctg
    15541 cttgaactcc agcaactctt ctgtaaatgg atggtaaaaa cgcagatcat aacaatgaag
    15601 ggcttgtctt tttattccca gatccattcg cccgccatac atatcatcac cgagcagggc
    15661 acagccaatt gcggaaaaat gaactcttat ttgatgtgtc cttcctgtat gcagctggat
    15721 atcaactaac gcgatttctg ttagtcgctt ctctatccag tactcagtct gtgccgaacg
    15781 tccttcatca gagaccattc gcttcaatag cgaagaataa tctcgggcga tcggcaaatc
    15841 gatatgtcca tgtgccgata aatctgaaag ccttccactg actaaggcct gatatttttt
    15901 cacaaacttc ttttcacgaa gttgcatatc taattttgcg tgcgcaaatc catgctttgc
    15961 aaaaagcatc aaaccagatg tatcccgatc tagtcgcgtc accacatgga ttacttggtt
    16021 ttcgtaacct tgttttttat aataagcctt tacacgatta gccattgtcc catttggatg
    16081 gtattgggca ggaatagacg aaacgcctgc tggtttattt actaccagaa cgtgttcatc
    16141 ttcatacacg atatcgatcg gtgtttcatc caaaagtacg gtttcatgtt ctccttcagc
    16201 aggaatcaca atggttacct tatcatcctt agctagagaa aacaagacat tttctacttg
    16261 atcattaact ttgatttgac cgccttgaaa ttttattttc gctaacagac cctttgagat
    16321 ccctttttct tttaaaaaat attttacttg ctgagaatga tctttctcat aatcccatgt
    16381 aaactgcata tcaatcctca ctaataaagg catctttcac acgatgccaa aaatgcatat
    16441 gtcgataaga agcaaaatgg atccgttctt ctgctattcg ataataaatt gagcggatat
    16501 ttgcttttgc gacattcaat tgatcaaccg ttacaagata atcttctgta ttttccaatt
    16561 ttatttctag ccattcatca tgggcaatca cgatcggaga acccaaagta cgaaacactc
    16621 gattattcag tgaagcaatc tctgccaact gaaaggcatt gatactagga tggatcacag
    16681 caccgccgac acttttatta tatgctgtcg aaccagtagg cgtagaaatc gtcaatccat
    16741 ctcctctaaa actttcaaac agctcgtttt tgatgtatac atcggctacc atcgtacgat
    16801 tggcccgttt aatcgttgat tcattcagtg ctaaaaaatg ctggtccggt gtttcatcca
    16861 aataactgat ccgaacatcc agcaagggat aactaacact ttgttcccgg ttcgttcgta
    16921 agctgtctac tagttcttcc agctcgtaat cccgccaatc cgtgtaaaac cctaaatgac
    16981 ctgtatgcac gcccaagaaa cgcacatcat tcagacaatg actgtagtga tggaacgccg
    17041 acaataatgt gccgtctccg ccgactgaga tgaccaattc tggctcatga tcatctcgtt
    17101 gaatccctgc ttgttctaaa agtgcgttca aacgttctgt cacttctata gtctttgctt
    17161 ctttattatg aacgatcgct actttcatcc cctgcctcct ctctttcgct ttctttcttc
    17221 aaaagcgttc gatagtagac atcatctgat tttcctcttc catgggagaa taaccgctgt
    17281 gcttcttgga tttcttcacg gatactggac atctcttcat ccaactgata ggcagcttct
    17341 gccgcacgct gaagtcgttc actcatttct tctggaaaaa cgccttgata tttataattc
    17401 agcgaatgct caatcgttgc ccagaaattc atcgcaagag tccgaatctg gatttccgcc
    17461 aaaattttct tctcaccgct gatcagctgt acaggatact cgatcaccag atgataagaa
    17521 cggtagccac ttgctttttt atttgtgatg taatctcgtt cttggagaac tttcatgtct
    17581 tttcttttac ggatgatctc aacaacttga tggatatctt ctacaaactg acacatgatc
    17641 cttaacccgg caatatcaga catttcttct tctaaacgtc ccattggaat atgccgtaat
    17701 tgtgattttg ttacaatact atcgactggc ttgactctcc cagtgacaaa ttcaatcggg
    17761 acatgtcgat tttgttcacg gaactgtttg cggatccccc gcagtttcac tttcaattct
    17821 gatacagttt gttcatatgg tgctaaaaat gcttcccaat ctcgttccat agctgatacc
    17881 tctacttcat taacattcat tgtttattct accatatatg caaagttttt tcgtactctt
    17941 gatttatgca ttgaccaaaa aaagaaaatt aggcaaaata gacaaaaagg agtcgatcaa
    18001 atgacacaaa tgcttgaaat cgaatataaa tctatgctga caaagcaaga gtacgagata
    18061 ttcatcgctc actatcaatt aacagataaa gatttccgtg tacagactaa tatttatttc
    18121 gataccgttg atgaacagct gaagaaacaa aattgtggcc tacggatccg tttcgttggt
    18181 catcaagctg aatatacatt aaaaacacct gctgaaaaag ggcgtctcga aacaacggat
    18241 acctttataa cagaagaagc agaagcattt atcttcgaaa aacgtctgcc tcgttcaggc
    18301 gcagtttttc aaaaactttc tgatttgaat atcgatcctg cttcattaat ccaaaccgga
    18361 aagcttacaa caaaacgggc tgaattccct attgaagaag gacttttagc tattgatgaa
    18421 agctggggag acaatctaca cgattttgaa ctggaactag aggtagggga tgcatctgtg
    18481 ggaaaaattg cttttataaa ttttttaaaa cgtttttctt taccttatcg tcctgctaag
    18541 aacaagatcc aacgtatgct tgaagctaaa aattagaaac ctttcatttt cacaaggcac
    18601 ttgagttcat ttttttactt ttttctgtta agttatagga tgtgaaagaa tctagtcact
    18661 agttgtatag aatctatttt gggggagatt catatgatcg aaatctattt atttgttaat
    18721 cctttagggg aatactgttt cgaaatggaa caacagttat tacaatttat cgaagaagag
    18781 tacgggacac catcaaaaga aaagatgcaa tttcgctttc ttcctttggt caatttgcag
    18841 acaattgggg atgtcatgca acaaaaagga atttctcaga acaatcttga ggaacgtaat
    18901 cacttattct caatgactta ttctgcagca ttggactgca aagcggcgca gtttcaagga
    18961 aagaaaaaag gccgccgttt tttgatcaaa ttgcaagaag cagtcggctg taatgacata
    19021 ccatattctc aagaacttgc tgaatccata tttgaagaaa tcggcggcga tttgaatatg
    19081 tttaaagaag atcgtcagtc tgacttcgtt aaagaagtat tcttttctga tcaaaaaatt
    19141 gctcgggaaa tgggcatcac aaaacaccct tctgcagtcg tttacaatta tgcctgcgac
    19201 cgtgattttg gtgttttagt agaagtaaaa ggtacaagta gcctttggag aatcacagag
    19261 ctgtgtaaaa caacagataa ctcatatcaa gtttttaatg gtgaacattt acatacaaat
    19321 gattctcagt tccattcaag aactagccat ttacacttgc tttctaatta aaaaaatacg
    19381 tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga aaatcaaaaa
    19441 tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt gttcaaacat
    19501 cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt ttttcattac
    19561 ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct ctagtgcctc
    19621 tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc cagtcgagta
    19681 ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga atttaatttc
    19741 tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat aactatcact
    19801 taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga aatgctcgaa
    19861 ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt aatggttgag
    19921 tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg ttaggatatt
    19981 ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt acacataagg
    20041 ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga caagcgtaaa
    20101 tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt catagcttcc
    20161 cgaagaataa gcatcgcttc gtttcccttt attttcgaca acatcgatcc aacgttcaga
    20221 gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg cttctaaagc
    20281 tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga ccggtgtata
    20341 aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt ccatgtatcg
    20401 atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata cactttcagg
    20461 aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga ctttcgcttt
    20521 aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc taaattgttc
    20581 gtaaacactg tacaatccct taaatgctgc ttcgcgcacc cttcgatccg tgctttctag
    20641 caactgacca tacacgccat gagataattg gactatttca ccattttctc cttcaatcgt
    20701 tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag catcaaagat
    20761 ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg ctcgattatc
    20821 tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt tgaaatattg
    20881 ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa cggcttcact
    20941 aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg tatttccagt
    21001 gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc gataaaccct
    21061 caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg ttcctttgtg
    21121 tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa actcttggtc
    21181 gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt ctcgttttgg
    21241 caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat ctatcaaaat
    21301 agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat tcatgtgtct
    21361 gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga ttcctagcct
    21421 atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta aaaaactgct
    21481 gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt tcttttattt
    21541 ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct tcgtacaaca
    21601 ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt tgatagatcc
    21661 acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt atggatactg
    21721 tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct tcactcactt
    21781 tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt tctttaaggt
    21841 ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat acagtctctg
    21901 taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag caatgcactc
    21961 ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc gattgtccaa
    22021 gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa ttttgggttc
    22081 tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct agattcgcca
    22141 acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat actggttcta
    22201 ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct tttgattctc
    22261 tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca cattttttct
    22321 ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc tttccttttt
    22381 ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc atttttctcc
    22441 ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg tgaaaaagct
    22501 attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt tttcgtttgg
    22561 ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa aagactgcga
    22621 cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa aatttcgttc
    22681 gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt tctggtgtac
    22741 gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca tccgcttggt
    22801 tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat aaatacaaat
    22861 cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt tcaaaatctt
    22921 caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat aattcttcct
    22981 ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct gaatcaacag
    23041 tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata aacagttcta
    23101 atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg aactcctctt
    23161 cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg tgattgccaa
    23221 gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt agtacacgga
    23281 tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg ccacaatgtt
    23341 taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat ctcgctgtta
    23401 agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat ttaactataa
    23461 catttttggc agacgaatac tatatagccg acttttcagc taattttccc tctctatttt
    23521 tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata ctttttgtaa
    23581 aaacaaaaaa acttggaaaa actatctttg t
//
LOCUS       DAFGFZ010000039        23593 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000039 DAFGFZ010000000
VERSION     DAFGFZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.039"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            37..1377
                     /locus_tag="LR726_001302"
     CDS             37..1377
                     /locus_tag="LR726_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6216403.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1595..2038
                     /locus_tag="LR726_001303"
     CDS             1595..2038
                     /locus_tag="LR726_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL6216404.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2542..3960)
                     /gene="nhaC"
                     /locus_tag="LR726_001304"
     CDS             complement(2542..3960)
                     /gene="nhaC"
                     /locus_tag="LR726_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL6216405.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4363..5586)
                     /locus_tag="LR726_001305"
     CDS             complement(4363..5586)
                     /locus_tag="LR726_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL6216406.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5617..6456)
                     /locus_tag="LR726_001306"
     CDS             complement(5617..6456)
                     /locus_tag="LR726_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL6216407.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6473..7153)
                     /locus_tag="LR726_001307"
     CDS             complement(6473..7153)
                     /locus_tag="LR726_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216408.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7155..8228)
                     /locus_tag="LR726_001308"
     CDS             complement(7155..8228)
                     /locus_tag="LR726_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6216409.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8284..8522)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8565..8798)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8875..9546)
                     /locus_tag="LR726_001309"
     CDS             complement(8875..9546)
                     /locus_tag="LR726_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL6216410.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9633..12311)
                     /gene="mgtA"
                     /locus_tag="LR726_001310"
     CDS             complement(9633..12311)
                     /gene="mgtA"
                     /locus_tag="LR726_001310"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL6216411.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12970..13407
                     /gene="ndk"
                     /locus_tag="LR726_001311"
     CDS             12970..13407
                     /gene="ndk"
                     /locus_tag="LR726_001311"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL6216412.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13429..14295)
                     /locus_tag="LR726_001312"
     CDS             complement(13429..14295)
                     /locus_tag="LR726_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL6216413.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKETSNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14455..16548)
                     /locus_tag="LR726_001313"
     CDS             complement(14455..16548)
                     /locus_tag="LR726_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL6216414.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17297..17920)
                     /locus_tag="LR726_001314"
     CDS             complement(17297..17920)
                     /locus_tag="LR726_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216415.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18079..19476)
                     /locus_tag="LR726_001315"
     CDS             complement(18079..19476)
                     /locus_tag="LR726_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL6216416.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19508..20479)
                     /locus_tag="LR726_001316"
     CDS             complement(19508..20479)
                     /locus_tag="LR726_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6216417.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20853..21278
                     /locus_tag="LR726_001317"
     CDS             20853..21278
                     /locus_tag="LR726_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216418.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21766..22467)
                     /locus_tag="LR726_001318"
     CDS             complement(21766..22467)
                     /locus_tag="LR726_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL6216419.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22483..22923)
                     /locus_tag="LR726_001319"
     CDS             complement(22483..22923)
                     /locus_tag="LR726_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL6216420.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22923..23498)
                     /locus_tag="LR726_001320"
     CDS             complement(22923..23498)
                     /locus_tag="LR726_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL6216421.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6786 a         4894 c         4062 g         7851 t
ORIGIN      
        1 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
       61 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
      121 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
      181 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
      241 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
      301 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
      361 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
      421 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
      481 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
      541 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
      601 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
      661 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
      721 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
      781 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
      841 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
      901 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
      961 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
     1021 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
     1081 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
     1141 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
     1201 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
     1261 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
     1321 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
     1381 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
     1441 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
     1501 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
     1561 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
     1621 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
     1681 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
     1741 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
     1801 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
     1861 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
     1921 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
     1981 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
     2041 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
     2101 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
     2161 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
     2221 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
     2281 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
     2341 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
     2401 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
     2461 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
     2521 tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg
     2581 cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac
     2641 aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt
     2701 acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc
     2761 atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt
     2821 aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc
     2881 gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac
     2941 tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt
     3001 tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga
     3061 atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt
     3121 cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa
     3181 caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg
     3241 aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat
     3301 agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac
     3361 aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc
     3421 aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac
     3481 ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat
     3541 accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc
     3601 tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa
     3661 cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc
     3721 gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc
     3781 gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc
     3841 tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa
     3901 aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat
     3961 gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact
     4021 ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa
     4081 ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac
     4141 ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag
     4201 cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac
     4261 gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct
     4321 gaaaattatt tttataactt tcacacacaa gtttattttt tactagaaag tagcgccctc
     4381 ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg
     4441 ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc
     4501 aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt
     4561 ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc
     4621 agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc
     4681 ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc
     4741 attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat
     4801 attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa
     4861 acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc
     4921 atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg
     4981 gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac
     5041 accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataacg
     5101 aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa
     5161 gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat
     5221 ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt
     5281 accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa
     5341 aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg
     5401 tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc
     5461 tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc
     5521 ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt
     5581 tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca
     5641 tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg
     5701 ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat
     5761 gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc
     5821 aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga
     5881 caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc
     5941 ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc
     6001 agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt
     6061 tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc
     6121 attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa
     6181 aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg
     6241 ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc
     6301 tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc
     6361 ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac
     6421 aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg
     6481 taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca
     6541 ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa
     6601 gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga
     6661 tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta
     6721 aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa
     6781 cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg
     6841 gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta
     6901 aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta
     6961 tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac
     7021 ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt
     7081 gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa
     7141 agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt
     7201 ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc
     7261 tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac
     7321 tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt
     7381 agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc
     7441 tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct
     7501 gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta
     7561 cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca
     7621 acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt
     7681 aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat
     7741 aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc
     7801 cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt
     7861 tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca
     7921 acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg
     7981 gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca
     8041 atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc
     8101 cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca
     8161 gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat
     8221 tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat
     8281 tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
     8341 ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac
     8401 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
     8461 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
     8521 aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
     8581 cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
     8641 attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt
     8701 ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga gcctctctgc
     8761 aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc
     8821 tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt
     8881 ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
     8941 tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa
     9001 aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc
     9061 aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga
     9121 aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt
     9181 aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt
     9241 catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc
     9301 tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt
     9361 ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttccgcc
     9421 ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca
     9481 gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc
     9541 tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa
     9601 aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata
     9661 tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat
     9721 aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact tgtaaaagga
     9781 aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg
     9841 ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat
     9901 agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta
     9961 tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag
    10021 taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata
    10081 ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc
    10141 aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt
    10201 ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa
    10261 cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg
    10321 cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca
    10381 atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca
    10441 ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa
    10501 cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct
    10561 aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga
    10621 cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca
    10681 gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca
    10741 aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca
    10801 acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt
    10861 gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg
    10921 ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt
    10981 tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa
    11041 ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca
    11101 tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac
    11161 gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg
    11221 attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag
    11281 ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct
    11341 ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca
    11401 gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata
    11461 actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct
    11521 aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt
    11581 gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc
    11641 ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca
    11701 tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt
    11761 gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct
    11821 ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc
    11881 gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc
    11941 aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct
    12001 ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc
    12061 aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt
    12121 ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct
    12181 gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca
    12241 aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt
    12301 ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa
    12361 aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg
    12421 tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat tttaattcat
    12481 gacttccaat catttttcga atagttattt ttccaattag accacctcct ttttattttt
    12541 caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg
    12601 aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg
    12661 taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt
    12721 tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc
    12781 acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca
    12841 aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca
    12901 cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga
    12961 ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta
    13021 gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt
    13081 gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt
    13141 tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc
    13201 gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt
    13261 ccaggtacga tccgaggaga ctacgcgctg ccgggaactg aaaacatcat tcacgcttct
    13321 gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac
    13381 aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg
    13441 ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata
    13501 ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga
    13561 aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt
    13621 atgtgataag ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt
    13681 ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt
    13741 caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg
    13801 atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg
    13861 tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt
    13921 ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc
    13981 tgttttctta aaatgcctac ggttattgct ggtctcttta taaggatcaa agctgtcttc
    14041 ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt
    14101 tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt
    14161 tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca
    14221 gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa
    14281 ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata
    14341 gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat
    14401 accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg
    14461 attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt
    14521 attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc
    14581 attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt
    14641 tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt
    14701 cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat
    14761 gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac
    14821 aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc
    14881 aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc
    14941 gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat
    15001 caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc
    15061 aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat
    15121 cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa
    15181 tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt
    15241 caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga
    15301 aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa
    15361 taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg
    15421 atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc
    15481 tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc
    15541 ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa
    15601 tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa
    15661 gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac
    15721 ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac
    15781 gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt
    15841 ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct
    15901 agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac
    15961 aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc
    16021 tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc
    16081 gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa
    16141 cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc
    16201 tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt
    16261 gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat
    16321 caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa
    16381 taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc
    16441 taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt
    16501 caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc
    16561 tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg
    16621 ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc acagcataaa
    16681 accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc
    16741 actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa
    16801 aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt
    16861 aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa
    16921 gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg
    16981 atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac
    17041 aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    17101 aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct
    17161 caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg
    17221 caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt
    17281 tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg
    17341 taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg
    17401 aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt
    17461 catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag
    17521 aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca acatgagtca
    17581 aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa
    17641 gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca
    17701 attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt
    17761 cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag
    17821 caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac
    17881 cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt
    17941 aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag
    18001 atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt
    18061 gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca
    18121 atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc
    18181 atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata
    18241 gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac
    18301 aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt
    18361 ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac
    18421 attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat
    18481 tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt
    18541 atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag
    18601 atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc
    18661 aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag
    18721 gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga
    18781 ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc
    18841 catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg
    18901 gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc
    18961 gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc
    19021 aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc
    19081 tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc
    19141 gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt
    19201 tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc
    19261 agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc
    19321 aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt
    19381 ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc
    19441 ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta
    19501 gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt
    19561 cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg
    19621 ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat
    19681 agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg
    19741 caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg
    19801 cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga
    19861 tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt
    19921 ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga
    19981 tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt
    20041 ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc
    20101 actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa
    20161 ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag ctatacccat
    20221 tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc
    20281 cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt
    20341 tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca
    20401 gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca
    20461 catcacggat cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt
    20521 gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat
    20581 aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt
    20641 gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc
    20701 tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt
    20761 gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata
    20821 atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt
    20881 tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct
    20941 cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg
    21001 tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag
    21061 ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat
    21121 ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac gaatatatta
    21181 tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc
    21241 gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt
    21301 tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa
    21361 tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa
    21421 tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca
    21481 tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca
    21541 aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt
    21601 gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa
    21661 agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct
    21721 acatcacgta gtaatttttc tttcccgtcg tttggttttt tatttttagc ctaattcttg
    21781 accttttgca tgctggttat ccgtaaaata ttttccagca ggctttcctt ccaattcagc
    21841 taatttttct tggatctgac ggatcacctt ttttgcatct tctggatttt ctacaaaatc
    21901 gaatttatcc ccatccactt gtattttagg acatacatca aaatcttcat accattgatt
    21961 atagcgggtg ttcaacgttt cataataggt atataacgtc tcatcgcttt ctagctgttc
    22021 atagtcacgc ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg
    22081 gaccatcaaa tcaggccgtt tttttggagc aacatcatct aactcgttta gcatcgtatc
    22141 taataagcta tcgtactgct gtacttcgat atccgttacc cgacctaaat cagcgtttag
    22201 atggaacaac aggctatctt catagatgga tcggtctagc acattgtcat cgttcactaa
    22261 agcatttttc attgctaaaa atcgcttgtt caaaaagaag atttgcaata aaaaagcgta
    22321 ctgctcagga tttgaataaa agagcggcaa tacttcgtta tcttcgacat tttcataaaa
    22381 tggtttagaa tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat
    22441 cgttcctgaa atcaaaatca ctgacataca acccttcctc ttctataagt actcgcctac
    22501 gtaagtttca acaggcaatg tttcaaaatc atattcacgt acagcttggt ctgattttaa
    22561 atacgtatgg agactttgtg taatcatcaa gttcgtaggt accgtctctt cttgtacaat
    22621 gatcatcggt tttttcaaca tagttgccac acctaattcg taagccgttc ctggatcgat
    22681 tgtttgatgc tcaaagtcaa ggatagcaac aacaaattct gcatttgtca gttcttccat
    22741 atctttttcg tatacttcct gcgcccatcc agcgctgaat agttcataat tgctttcttg
    22801 gtgatgacgt ggagagtaga aacttgtgac tgttttgttt tcctccaacg ctttttcgat
    22861 acgacttact cgatcgattt gctcctctga aaaaaatggt cctgctaaat atatttgact
    22921 cattattttg cctccttcaa taataactga tttttaatag atactgctcg taattcattg
    22981 accagttcca atggattgtt ggaaaagatt acttgatcca accatttttt atccataaaa
    23041 tcctcttttg caaaagtttc taataattgt tgcaagccgc tgtaataacc gccaatattc
    23101 aatattccga ttggcttgtc gggcgtcaga ccgattgcta tccaagaaag aagttgtgag
    23161 aattcctcca atgtacctag acctccaggg aaaacgagga cagcatctcc cttttcaaat
    23221 aataatacct ttctttcatc gatcgtttct gtaggaataa atgtagtaac atcttttcca
    23281 ggcaattcag tttcaaaaag cccattcgga tagatacctg tgacttttcc gttattttcc
    23341 agtactgttt tactaatcac tcccataatc ccggagtatg aaccaccgta aaccagttcg
    23401 atttcttctt gtgccatgta ttttcctaac gtttgcgcaa taaatttgta actttcttta
    23461 ttaccaaaac gggaaccgca aaaaacagta agtttcataa aaaaacactc cctaactcaa
    23521 aagtctatct tcattctaca cgtgtttttt taaaataaaa gactagattt tgtagttttt
    23581 tagtggtata tag
//
LOCUS       DAFGFZ010000040        23585 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000040 DAFGFZ010000000
VERSION     DAFGFZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.040"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LR726_001321"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LR726_001321"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL6216422.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LR726_001322"
     CDS             complement(1172..1867)
                     /locus_tag="LR726_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL6216423.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LR726_001323"
     CDS             complement(1880..2596)
                     /locus_tag="LR726_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL6216424.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="LR726_001324"
     CDS             2926..3534
                     /locus_tag="LR726_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL6216425.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="LR726_001325"
     CDS             3859..4182
                     /locus_tag="LR726_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL6216426.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="LR726_001326"
     CDS             4364..5734
                     /locus_tag="LR726_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL6216427.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="LR726_001327"
     CDS             complement(5864..7165)
                     /locus_tag="LR726_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL6216428.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="LR726_001328"
     CDS             complement(7519..9477)
                     /locus_tag="LR726_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL6216429.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="LR726_001329"
     CDS             complement(9654..9920)
                     /locus_tag="LR726_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL6216430.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="LR726_001330"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="LR726_001330"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL6216431.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="LR726_001331"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="LR726_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL6216432.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="LR726_001332"
     CDS             13264..13827
                     /locus_tag="LR726_001332"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL6216433.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="LR726_001333"
     CDS             complement(14080..14601)
                     /locus_tag="LR726_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL6216434.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="LR726_001334"
     CDS             complement(14604..15446)
                     /locus_tag="LR726_001334"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL6216435.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="LR726_001335"
     CDS             complement(15439..16164)
                     /locus_tag="LR726_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL6216436.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="LR726_001336"
     CDS             16340..16636
                     /locus_tag="LR726_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216437.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="LR726_001337"
     CDS             17204..18796
                     /locus_tag="LR726_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL6216438.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="LR726_001338"
     CDS             18809..19549
                     /locus_tag="LR726_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL6216439.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="LR726_001339"
     CDS             19717..21066
                     /locus_tag="LR726_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6216440.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="LR726_001340"
     CDS             complement(21194..21784)
                     /locus_tag="LR726_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL6216441.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21931..23283
                     /gene="brnQ"
                     /locus_tag="LR726_001341"
     CDS             21931..23283
                     /gene="brnQ"
                     /locus_tag="LR726_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL6216442.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6744 a         4757 c         4497 g         7587 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattgag aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtatctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 atcaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acaaacttcc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgccatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 aacaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa aacagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagattact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct tttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagttaa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccgtcgtt ttatatagga aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
    21961 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
    22021 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
    22081 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
    22141 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
    22201 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
    22261 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
    22321 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
    22381 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
    22441 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
    22501 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
    22561 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
    22621 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
    22681 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
    22741 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
    22801 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
    22861 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
    22921 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
    22981 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
    23041 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
    23101 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
    23161 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
    23221 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
    23281 tgataaaaga ggttgtgaaa gagacgttca gccttattgc cgataggctg tctcttgaac
    23341 actgtttatt gagaggtcgt gaaaaagccg ctcagtttcg agtattaaga aatatttttt
    23401 gaaaatggct tctcacattt ttgcataaat ttgacttaat accgagaaac tggcttttga
    23461 acaccgttta ttgagcgtct ataaactgac ctgagtatta gagaaacaaa acggcaaagt
    23521 agctccatat actttgccgt tttgttttga actaataccg aagagaagct gatcaaacaa
    23581 cattt
//
LOCUS       DAFGFZ010000041        23069 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000041 DAFGFZ010000000
VERSION     DAFGFZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.041"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(193..648)
                     /locus_tag="LR726_001342"
     CDS             complement(193..648)
                     /locus_tag="LR726_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216443.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(802..2010)
                     /locus_tag="LR726_001343"
     CDS             complement(802..2010)
                     /locus_tag="LR726_001343"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL6216444.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2122..2454)
                     /locus_tag="LR726_001344"
     CDS             complement(2122..2454)
                     /locus_tag="LR726_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL6216445.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2602..3477
                     /locus_tag="LR726_001345"
     CDS             2602..3477
                     /locus_tag="LR726_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL6216446.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3559..4140)
                     /locus_tag="LR726_001346"
     CDS             complement(3559..4140)
                     /locus_tag="LR726_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL6216447.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4146..5405)
                     /locus_tag="LR726_001347"
     CDS             complement(4146..5405)
                     /locus_tag="LR726_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL6216448.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5407..6453)
                     /locus_tag="LR726_001348"
     CDS             complement(5407..6453)
                     /locus_tag="LR726_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216449.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6688..6963)
                     /locus_tag="LR726_001349"
     CDS             complement(6688..6963)
                     /locus_tag="LR726_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL6216450.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7206..8516)
                     /gene="der"
                     /locus_tag="LR726_001350"
     CDS             complement(7206..8516)
                     /gene="der"
                     /locus_tag="LR726_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL6216451.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8698..9927)
                     /gene="rpsA"
                     /locus_tag="LR726_001351"
     CDS             complement(8698..9927)
                     /gene="rpsA"
                     /locus_tag="LR726_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL6216452.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(10062..10742)
                     /locus_tag="LR726_001352"
     CDS             complement(10062..10742)
                     /locus_tag="LR726_001352"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL6216453.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10791..11426)
                     /locus_tag="LR726_001353"
     CDS             complement(10791..11426)
                     /locus_tag="LR726_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL6216454.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11492..12895)
                     /locus_tag="LR726_001354"
     CDS             complement(11492..12895)
                     /locus_tag="LR726_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL6216455.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12882..13913)
                     /locus_tag="LR726_001355"
     CDS             complement(12882..13913)
                     /locus_tag="LR726_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6216456.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13944..14165
                     /locus_tag="LR726_001356"
     CDS             13944..14165
                     /locus_tag="LR726_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL6216457.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14330..15067)
                     /locus_tag="LR726_001357"
     CDS             complement(14330..15067)
                     /locus_tag="LR726_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL6216458.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15220..16560)
                     /locus_tag="LR726_001358"
     CDS             complement(15220..16560)
                     /locus_tag="LR726_001358"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL6216459.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16797..18320)
                     /locus_tag="LR726_001359"
     CDS             complement(16797..18320)
                     /locus_tag="LR726_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL6216460.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18698..19309)
                     /locus_tag="LR726_001360"
     CDS             complement(18698..19309)
                     /locus_tag="LR726_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL6216461.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19432..19557)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19639..20355)
                     /locus_tag="LR726_001361"
     CDS             complement(19639..20355)
                     /locus_tag="LR726_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL6216462.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20361..20930)
                     /gene="scpB"
                     /locus_tag="LR726_001362"
     CDS             complement(20361..20930)
                     /gene="scpB"
                     /locus_tag="LR726_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL6216463.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20914..21711)
                     /locus_tag="LR726_001363"
     CDS             complement(20914..21711)
                     /locus_tag="LR726_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL6216464.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21681..22052)
                     /locus_tag="LR726_001364"
     CDS             complement(21681..22052)
                     /locus_tag="LR726_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL6216465.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(22071..22958)
                     /gene="xerD"
                     /locus_tag="LR726_001365"
     CDS             complement(22071..22958)
                     /gene="xerD"
                     /locus_tag="LR726_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL6216466.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6474 a         4931 c         3914 g         7750 t
ORIGIN      
        1 aaaaaatctg agccagaatg aggttcattc tgacccagat ttgattttgt acaacccatc
       61 agtccttatt gacaaagagc ccaaaaatct tgtcacagtc ccttattctg aataaacggt
      121 gtggaagaga cagcctatca gtagtaataa gacacatctc tttcacagcc tcatttttgc
      181 gtaaaaatcg atttattcga aaaattcccc gtatacatct tctacacgtt gatctggatt
      241 gatcacgata tacaagttac ctgttttttc gctgattttt gttgattgat attgattgtc
      301 taatccttct gttcctttat ctagatatgg gaagtacaca agatcaggtg tgccatttcg
      361 gtaaaggatt tccattcgct cgatatcttc gtatttcaat gcgcgattaa acatgcccat
      421 ttccagtccg cctaggttga tatcatgtgt cgcaactgcg tccccttcat ggaagatttc
      481 gattttaaat ccagcacacg gatggatttc aacaaattca ctgccattga ttcgtccaaa
      541 acttgttgtg atttgtttga tccaaagatc gccgatatga cggcggttga tcgtccaggt
      601 ttctccattt ctaaaataaa attttagcgc tgtcatttct ttactcatta aaaagactcc
      661 tttatgatgt aatttattct tgtactacct cagtttagca caaatttatg taagtggtca
      721 catcttatgt tttttcttct aatgacgtat ctgcccactt taagtagcca acaaagctga
      781 gcagatacag tctagtgttt tttaaatcaa tttttctttc gataaaaaat ccaaaacctg
      841 ttttttatca ttttttattc tttgttctac gacggctttt tcagctaatt gcaaagcttc
      901 actaacccac ttaccaggtt tttttccagt ttcttctagc aagattttac catctactgc
      961 taattcgctt aatgacttga taggtaaacg atcataggat tctcctacgg catccatctg
     1021 aggtgtttcg tcaaaataga tcatcaattc ttctacttgc agtgcatgtt ttttgcctaa
     1081 gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc cttggacaag
     1141 tgcttgaacg tctttaatca tttggttaga acacttccac gatttcaaaa aggaacgaat
     1201 ctcattttct tttagatcca gttggctgat caataatgcc catgcttggc tctcttcttt
     1261 caatgggacg gatggtaatt cagcaaaacg taaaagtgct tcgccttctt ctttcaaacc
     1321 gggacagcaa atgtagcact ctgtttcaat aaatgcatgg attcccgcgg cccgttcatt
     1381 tcccaagagc atcttgatga attccacatt tatacgttct acagatattt tacctaatag
     1441 ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct aatttgaacc ctagttgact
     1501 gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca tggaaccgct catgaggatt
     1561 gcctacagct cgcaagatct gttcttctag atcttgcaga ccatcgaaca aatcgatgat
     1621 ttctccatct tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt tcaaatcttc
     1681 tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg tagtcctgat aagttgattc
     1741 cgtccgaaat gtggtgatct catattgttc atcattttct aataccaaca cagttccatg
     1801 ttcgatccca atatctatcg tttttggaaa aagctgtttg atttcttctg gaaatgcgct
     1861 tgttgcgata tccacatcat ggattggctg gttcagcaag atatcacgaa cacttccacc
     1921 tacaaaataa gcttcatatc ctgcattctc taattttttc agaactggaa ttgccttttg
     1981 gtattccaga ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt gtttcttgtc
     2041 ctgagataaa gactcagttg catggtgact gtttgacaag tccgtttcag agtcttcaag
     2101 gccaggacca cttcatcttg tttactcgtc cttacgctca aagcgattat ggtctctttg
     2161 attgaatttt tccatgtttt tttcaaaaac ttcagttaag tcgatattca atgaattagc
     2221 catgatcatc gtaacgaata aaacatcacc aagttcttct gctaccgagt taactggttc
     2281 agaagccttt ttgctttttt ctccataagt gtggttgact tctcttgcca attcacctac
     2341 ttcttcagtt aatcgagcca tctgagctaa tggagagaaa taccccacct tgaattgctg
     2401 gatatattca tccacttcct gctgcatact gtataaagaa cgctttttat ccatcttttt
     2461 ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct agataagagc aattgagaaa
     2521 atcgaatttc catgctacat ttaggaagga atttctaaat ggaaattctg tgaaaataca
     2581 taattgaaag ctggaattca tatggtcaaa cgaaaaacag taaaagaagt tctttttatc
     2641 attataggaa caagtattta tgcatttggt cttgtttact tgaacatcgc taatcattta
     2701 gcagaagggg gagtctcagg gatcaccttg atcttaagag ctttatttgg tattgatcca
     2761 gcttatacga ctttattaat caacatccca ttgattttac taggaggaaa gatcctcggt
     2821 aaacgttcct tggcctatac tgttctaggt acactttctt tgtcattctt tttatggatc
     2881 tggcaaaggg ttccactaga aatcaattta cagcatgatc tactgatcgt ttctctactt
     2941 gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg aacaacaggc
     3001 ggcagtgata ttatcgctcg gattttagaa caaaattacg gcatcactat ggggcgttcc
     3061 ctattggcat ttgatatcat cgtcttactt gcttcattaa cctatatcga cttgaaacgt
     3121 atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc gatcttagat
     3181 ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga gattgcacca
     3241 ctactgatga ccggtctgca aagaggtgtg actttccttt ctggagaagg cgcattctct
     3301 ggtaattcaa aaaaaatcat ctatatggtc gttagctcta gtgaattaac tgaagctaag
     3361 aggatcgtcc acgagattga cgaaaaagct ttcctttcaa tcatcaatgt gcatgaagtg
     3421 gaagggcaag gctttactta tttgaagcca cagacaagat tactgaaaaa actataagaa
     3481 aaaagacaaa gataggctat gaggaaagat tccacatcca gctgaccttt gtctttttta
     3541 gtactatact atttttaact aattgttatg ttcacttacc cggttacttt tcttttgtgt
     3601 ctgcaaaagg atctttcggt taacttcatc agataatttc aaaaaagcat ccctatctcc
     3661 atcttctagt gcttgatcga tttgagcata aagcattttt agttttgcct cttcttcttt
     3721 ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca ctgactgaag aatcccaaga
     3781 agaaaatgga ttgtcttcta agatagatag atattctctt gtctgccaag cattttcgaa
     3841 tatacattcc acatataaag gctttttcca attcaaacgg atttcatgaa aaatctggtc
     3901 tgtgtccgta aattcttttc cctgcaaaaa caatttcatg ggttcccctt caacagatag
     3961 atcactgatc tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt ggacattgct
     4021 taaaatcgct tcatgattgg caagataatt caagatccaa aaaacttctc ttcggctaaa
     4081 agatacatga ttgaccatcc acgttaaaaa attcttcttt tcccgtacat caattagcat
     4141 ttttctcacc tctctgttag gtcatctaat aatgacagtg cctccatatc tcctggttca
     4201 agtgttaaat aataagtcaa taattcttgc gctcttttca gctgcccttc ttctcttaag
     4261 aaaaaggcat actctttcaa gaaatctggt tcatgtgata gttcatgata agcctgttca
     4321 tagtgtacag ctgcaacggt aaagtcttct aattcattat aagcatgggc taggttccat
     4381 tctgcataag gattagcagt ttcttccatc tgattgatca cttgtatcac atcttcatat
     4441 ctttcatcat ccaagtaaag attacttaaa gtaagtaatg tttcatcttg tttgtctcct
     4501 acttctaaag ctttcagtaa aagttcttct gctcgctgtc tatcatgcag acggaaagca
     4561 ttttccgagg ccaggtgata aagttcgact tggtaaggat tttctttgat tccttcttct
     4621 aacactgttt gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc tagatataga
     4681 tataacgatt gataatgcgg attaacttct cgaagttctt ccagatagtt gattgcctgc
     4741 tgattttctt tcaattgaag attcgtaaaa gctagctgga acagtcgatc atctgtcctt
     4801 tcttctttca gtgcttcttc taaaggtacg atagctgctt cgaattctcc ttgcatactt
     4861 aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa tttcatttat cccgcttgcc
     4921 aacaatgttt cataaaccac tgctgcctct ttgaaacgat caactgaaaa atacaattca
     4981 gctagcgcaa actggatcaa tggttcatca ggaagcaacc cagctgcttc tttcaatttt
     5041 gcttcactca cttcagggat tccgataact tggtacaaat cagctatcgc caataagctt
     5101 tgcggatagt aatcactttg cttatccact ttttccaggt attcaaaagc taagtcgatc
     5161 tcattatctt cgattgcaat ttctgctaac gggatgttca gtccgtcatt ttctgggtat
     5221 tggttgatca gttgttcaaa aatctgtttt gcttcgatca aaaagccaat agccagcaat
     5281 tcttcgccta gttcagctaa tatatctgct gggtcttctt ttaatgcttt ttctaaaaag
     5341 atttgtgctt gtgacaagtc ttcatctgat aaagcttgca acattttttc gctatttgta
     5401 ttcatttcaa ttccctctta tctcactgac gatttgttga aataccgtca ttgttctcaa
     5461 taattctttt tcgtttggat gttggtatcc gtctattata cctatttttt ctaaaacaag
     5521 tatttttttg cttttttctg ccaaaaaaag aactttttcc aaatactcca tctttgaaat
     5581 ctgttccagc cagtggattg gatagccaag caagtccagc cacacaaaaa agttttttag
     5641 ttggaaatca aaaggatatg cttggagatt ccacacgaaa tgaaacatca ttcctaaaaa
     5701 ttttttcata ctagcagaaa gtagatggcc attttcggtc agataaaaag cttgttcaaa
     5761 cagtttacca taatttgaca ggttttctgc ctctaattga taaaaataga tcatttcttc
     5821 gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta tttggatagt ttttatacag
     5881 attttgcaaa aaacgatgat cacaaatcat tccgcaacag ataaaagtca aaaagtcaat
     5941 ctgctttccg tccacctgcc gctcgctgat tgtttgatca taaacgatgc gttctggcag
     6001 attgatggtt tgcagcacta cacgatttgg tagctgcaaa atcgtccgct gcggtgttaa
     6061 tgctcggctt agagaacgga tcgatacagg gatcatccat agtgatgaat caagggttgt
     6121 atgtttttga aaaaaacctg ccagatccat gatcccttca tttccaaatc cgatgataaa
     6181 catggattga ttttcgggat agcgtttgac gaaatcaagc aggttcttta attctgttaa
     6241 atgattgcat tgagtgttgg cacaaatata ccagtcattg tttgcttgat ttgcaaataa
     6301 cgggttgatc ttatctgcaa acaaatcata atagcgctga ttcgttaaaa ataagattgg
     6361 ctgatcaatg gaaagttctc ttagctgact gactagcgtt tctccgtaaa acactttcgt
     6421 caacgcatct tctacttgat aatcaagttc catacgcatc cctcctcttc ttatttttat
     6481 tttaccatta gtaagcaaat tctagaaaag ataagagcaa agctcacctc ataacttgga
     6541 aataaataaa aattaaataa aatacatgtg ttttctatgt atgacatacg gattcggaat
     6601 ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac ttgtagccct
     6661 agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg ctttacctgg
     6721 tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt gtgggttacg
     6781 acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta attgaacttt
     6841 ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg ctgcagttgc
     6901 atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg ctttgtttgc
     6961 catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta gttaaaactt
     7021 tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc tgttatcacg
     7081 atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct ttttttagtc
     7141 atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa
     7201 aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa agtaaatgct
     7261 tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc ctcttcattg
     7321 acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa aatcttcagg
     7381 cgttttcctt tatctgttgg tgttggattg attgctacag catccataat gatatcattc
     7441 aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat catttcaggc
     7501 agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc gtaatcaaga
     7561 taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt ttctttcttt
     7621 acaagatccc atttattcac gacgataata attccacgac cagcttcatg ggcgtaaccc
     7681 gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat caaaacgata
     7741 tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt attttcgtat
     7801 acttttccgc gtttacgcat accagctgta tctatcatta agaatttctg accactttca
     7861 gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga aacaattaca
     7921 cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg gcgaccaatc
     7981 aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa atacttcact
     8041 gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg ataaggatcg
     8101 cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt atctacttta
     8161 ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac catctcatct
     8221 gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc ttcttcaatc
     8281 gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag atcaattccg
     8341 cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata aatacgatca
     8401 cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat gcggttaaaa
     8461 atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt tgccatatta
     8521 tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc aactttcaag
     8581 catagattgt caattaaacg aagtattttt attccttatt gaaatactaa tcgcttttca
     8641 aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca aaatcaatta
     8701 tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat cacctaaaat
     8761 gtctcccata gtaaatccag tattttcttc tggtaattca taagactcaa cattttttgg
     8821 ttcttcttct gaagctggtt tttcttctaa agctttgata ctcaatgcaa tacgatgttc
     8881 ttctggatgg atctctaata ctttcacttt cacttcgtca ccttcatgaa gcacttcatg
     8941 tggtgtcgca atatgtttat gtgaaatttg agagatatgg actagccctt ctactccagg
     9001 gaacacttca acaaaggcac cgaagcttgt caaacgttta actgttccgt ctaatactgt
     9061 gccaactgga gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt ctttgattga
     9121 caaggaaaca cgttcttgtt caggattgat agacaacact ttgacattta catcatcatt
     9181 gacagccaat acatcacttg gttttgctac atgactatgt gaaatctctg agacatggac
     9241 tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg
     9301 tccactgaga acatctccct catggatagt tgaaagaatt tcttttttct tttcttctct
     9361 ttcgttttcc actaccgctt tatgcgataa gatcaaacga ttttctgatg gttcgatttc
     9421 gatgatttta aagctcaatg ttttgccttt atagtctgaa aagtctgcaa caaaatgatc
     9481 ttccaccatt gatgcaggga caaaaccacg tacaccgacg tcaacgacta aaccgccttt
     9541 gacaacattt gtaacaggtg cttcgatgac cttgccctct tggaaatctc tttcgatatc
     9601 ttcccaaact tttttagcat ctaaacggcg ttttgaaagt aggtagctgc cgttttcttt
     9661 atctttgccg atcgtactga taacgactaa atctaataca tcaccgactt ttactacttc
     9721 gctgatatct tctaccggca aagttgataa ttcttttgcc ggaacgactc cttcaactcc
     9781 agccccttcg attccgacaa tcacttgttt gtcttcaatg actaaaactt cgcccttaac
     9841 aacatcgcca acttttactt cttgtacgct tttcattgcg tcttccattg actggttgtt
     9901 ctcttgattt tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca ttctgccgta
     9961 agtacggtac tgtttttagt ttaattgatt attcaaaaaa aataaagcga tttcgttcgt
    10021 tttccttgaa aaaacaattg tttttcattt tttttcttaa tttaaaaaag aagataacct
    10081 tttttcaaaa caacagcttt tattgctgca acgacttctg gaatcgattt gccagtcgta
    10141 tcgatacgaa cagcatcctc tgcttgcttc agcggagaga cgtcccttgt tgaatcataa
    10201 tggtctcttt cctgtatctc tttcgtcagt gtttcaagat cagtcggaat tcctttttct
    10261 tggttctctt tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa aaagattttc
    10321 acttctgcat tcggtaatac agctgtcccg atgtctcttc cgtccatgac aacgccaccg
    10381 cgttccccta tttttcgttg gacagcaact aattcttctc tgacttttcc atgtgcagag
    10441 acttcagaaa cagcttttgt gacatctggt tgacggattt ctaaagtcac atcttttgat
    10501 gctatgaata catgctgtcc atcttcttgt tgcgaaaaag agataggata ctttttgatc
    10561 agcgaaacta gcccttcttc atctgaaaaa gatacattgt tcgtaatagc taaataagtt
    10621 actgcgcgat acatggcacc ggtatctgta taaatataat tcaattcttt tgctaatatc
    10681 ttggcaacgg tacttttccc agatgaagca ggcccgtcga ttgcaatact gatttgtgtc
    10741 atgatataac tcctttttct cagaaaaaaa gccgcaaagc gcggctatta ttatttaatt
    10801 cttaattctt gaccagggta aagcataaag ttgtttggat ctaagccatt caattcaaac
    10861 aattgatcag tggtgatccc tgcacgctca gccacttgtt ttggaccttc gcctgcttga
    10921 acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc tgcattgctg
    10981 cttgaatccc ctgcttgata agttgcctca cttgaggaag tcggtgttgt ctctgctgca
    11041 atactgcttt ctgacgttac acctgtatct tgagacacag tagattcttc cgcgattgta
    11101 ctagattcta ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt agaactttct
    11161 actactgatg tcgaagtttc agaagaagtt gctgctgttg cattgttgct tgatccattt
    11221 ctgatccaga aataagcacc tgctggaata gcgatacata agaccagtaa aatcaaaaat
    11281 agcgaaagaa ataatgtatt tcctttcttt tgttgtctct gagaacttct tgatgaaact
    11341 tcatcatctg tatcgtagat tggttgttcc catggttctt gtgtttcgtt attttctgta
    11401 gattgatgtc tatcttttct gctcactgtt ttacctccta aactctatcg tgtgtattat
    11461 accataggaa aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt ttaaataaat
    11521 ttagtaaaat cgtttcccaa tgaagttgtt cagtcttaga aaaaagcttc ctctttggca
    11581 gaagttctgc ttttgctcca tcaatatcac aacaattctg cggcttttcg gagagttttt
    11641 cgccaaaata agctaagatg aattttctgc ggcagttttc ttcattaata tagcgaagca
    11701 tctgctgcag gcggaattct ttttcttttt cctgacgttt gagccgttcc acccaatttt
    11761 ctggtttttc agactgctgg atcagctcca tccatttttt ttgcagctca tcaaagggag
    11821 cttgctttgt ggcatattcc aaataaagct caaaactttg tcgctgttct ctagaaagtt
    11881 gattaaagaa ataatggata cgttcatccc cactttgata aaaaagaatt gctgcacttt
    11941 cttcctgatc tctgcctgct cgaccgattt cttgtacata gttttctaga ctgtctggca
    12001 aatcataatg gaccacataa cggatatcag atttatcgat ccccatacca aatgcattcg
    12061 tagcaatgag aaactgcagc tgatttttta caaactgctg ttgaagttgt cttctttgag
    12121 ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg ttccctgaat aaatgataca
    12181 atcgttcgac ttcttttttt gttgcacaat aaatgattgc cgctccttgt gctctttcca
    12241 tgaagtgttc aagttcttgt tctttttgct gcgttttccg aacaaaaagc gagatatttt
    12301 gccgattgac tgattgtctg atctcttttg gtggtcgatc aaatagtaca tgacgtatat
    12361 ctcttgctac taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa
    12421 gctttcggat ttttcctaac tgttgatatt ctggacggaa atcaactccc cattgagaca
    12481 cgcaatgagc ctcatctact acatataacg caatattttg ttttttcaga tgctctaaaa
    12541 gagaaggatt agtcaacatt tcgggactca aaaacaaaaa tttatattgc gatagatgac
    12601 ggaggacgta tcttctttca gattgcgaaa gtaaactatt gaaagcagct actcgtttct
    12661 cccctcgttt ttgtaattgc gtcacttgat cttccattag agaaagcagc ggtgacacga
    12721 tgatgaccag accttccatt aaataccccg tcaattgata gcaaaggctt tttcccgttc
    12781 ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa cgcctgaata atttcttctt
    12841 ggccgggacg aaatgaatca aatccaaaat atttttttaa ttcatttatt aacattctgt
    12901 atcgcttcct ttctcaatag atagatttga tacaaacgaa attctccata atctaattgt
    12961 ccatctacat tccattcgct aaagcgccac tctcgcacgg aatccttatt tggttccaaa
    13021 cgttcgattg tttctgcaga gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc
    13081 cactcaataa gatgatctgt tactgttcct ctttttaaat gacggattgc catgatctgg
    13141 tcaattgtct gaccattcat aaacatctgc ttggtgatca tcatgctttg attgaaattt
    13201 tgctgtaata atgaatcgat caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt
    13261 gctaacaaaa ggtggataca tctgctttgt tgcagataaa cctctgactc atcattgttt
    13321 tttgccagct gataaggaag cagccctgtt cgctcatttc cagataattg gttagctaaa
    13381 aaatctgctg cttcttctgg tatctccgaa aacagctgca ccaaactctc atacgcagta
    13441 tcaattaaaa tttcacgtgg caattggctt tcactaagcc atttttttag ttggaaagta
    13501 taaaaaggac ttgtctctgc tggaagatag tcttgaatac ccgttgccag attagaaatt
    13561 acttgaacag caaatttaat gagccgccaa ctagtttccc ctgttctgcc atagcggtca
    13621 aaacgcaaac cagtatgttc tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt
    13681 agctttgctt cattttcatt tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt
    13741 tgataaaact tatcctgctt caattctgga aaactgttat ggataaacaa aagttcgtgg
    13801 gtaaatccat aaataagtac tgaagtcgtt cgttttccca caaggagttg gtaaagagaa
    13861 ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat catattattc
    13921 tccttaagtt aggatggtta gaaatgtcac tactatgtaa acttgtacct gaacgctgta
    13981 ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt cgattatgat gaagaaggaa
    14041 tcgttttatt tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa
    14101 catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc gattttatta gaaaataaaa
    14161 tataaatata aacgaaactt taaaaacgta cactaagttc tctaaacttg aaaataagcc
    14221 gggatctgtt tctcttgttc cgtgaaatcc cgacttattt tgaaagattc atttgctatc
    14281 tatagttaat tgactagttt tttcacggac cctataggac ccatttatat tatttcaact
    14341 ttttcagcca gttttccaat gtattgacga tcacagtttg ctggtctgcg ttagaaattt
    14401 ctgcctctcc atctcctttt tgctcaccat agctgccaaa acctccatga tttccgccat
    14461 tgattgatac atactctgtt ctttccggta aatactgctt agcttcttga taagcttcct
    14521 gattcaatac tttatctttt gtcgccgtga tcgataaaac aggaacagct gattctgtca
    14581 atgttccttt tttgtcggga taacttgcca tgaagaatac cccttttaaa tgtttgtctg
    14641 aatgattctt attgacataa cgacttgcca ttacgccacc taatgaatgg ccaccgatca
    14701 cataatcgga atttggaaaa tcttctttga ccttatccgc tttgtttccg cccatcactg
    14761 ctagatcaag tggcattttg acaatagcga ctgaaaagcc gttttttgct aaatcttgcg
    14821 cccaaatact ataactttct ggctctacga gagcaccagg ataaaaaata agtaatggag
    14881 actttccatg attctttttc tctccttcaa aaaataagta atcttctttg ttttttcctt
    14941 cttttaccaa cacttgcgct tctgacgatg gttcgtacgt attttgctgt atatagtaat
    15001 aaccggctcc accgagtaat cctaaaatga tgactaagcc aattagtatt cgtctaaata
    15061 ttttcacttt ctcccacctt ttctatctga gtgacccctt tttcttaatc aagtatgatt
    15121 ataccttaaa aaaataagag agaaatgcaa actttccagc atcaaataaa aaaagacgga
    15181 atatccattt gaatatccgc cctttttgta tgtttatttt tatttagcac ataaacgata
    15241 cacagcttct gcataaattt ccattgcttt atacatacta cttaattccc atcgttcatt
    15301 cgcctggtgc ataaagtcag gagtatcagg gaacatcgca ccgaatgcca cacactggtt
    15361 catcgtacga gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt ctccagtaat
    15421 atcgcgatat gtccccaata gatttttcac taactcactg tctagtggca cgtataatga
    15481 atcaaggaag tcaaattctt cataagtcaa cccatatttt tctgctgttt tagttaattt
    15541 agctactaaa tcatctttct tgaatgtaac agggattctc atatctacac cgatttttgt
    15601 ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg attgttcatc
    15661 ttctgttttg cccacgacag cttcaccagt tgcattttct tgaactaatt ttcctaagaa
    15721 attgattggg ccaaaatcaa atacttctga aagagcaatt gccagacgag aaatagcatt
    15781 cactccttgt gctgcatctt tcgcgtggat acttttacca agaacgacga tgccttcacc
    15841 ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct tttacttcag aaagcttttc
    15901 tccagagtaa ggagctgcat caggaacaac attcaacgct cctccagctt ttacagaaaa
    15961 ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag cctttttctg cataaattaa
    16021 aggaaattca gcatctggcg caaatccttg cgtaattcct tcttcttttt cgttatattt
    16081 ttctaaacaa cgccataaat tttcttcatc tgtaccaaag ataaaacgaa tacgtgtctt
    16141 gaattccacg ccagcatcaa tcaaagcttt gacggcatac attgccgcaa tagaaggacc
    16201 tttgtcatct tgggatccgc gaccaactaa ccagccgtct tttatagttg gatcgaatgg
    16261 ttttgtttcc caattatttt cgtcccctgc tggaacaacg tccatatgac acaagatacc
    16321 gaataattca tctccagagc caatctctga ataaccgtaa tagccttctg gatctttaaa
    16381 tgttctaaat cctagctttt cactgatttc caatacttta tccaaagctt caatcacttt
    16441 tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct agtacggaag gaattcggat
    16501 caattcgctc aatgattcta atgcttgttc atgatgttgt tctgtcacaa attttttcat
    16561 gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac acaacctctt
    16621 cttctcaaat aatgacgttt taagcctgct ccatcagcaa gcagaatgtt cttaacaaat
    16681 gatgaaagaa aaaacactca ctgaatggag ctattgactt gtccacgtgc ttatcgatag
    16741 ccgtttatcg gtttcttcat tcttatgata gaccttgtac ctgtatttgt ctaggtttag
    16801 aacattgctg ctacacctag gatcacacag atggctacaa ataatatcgc aattaattta
    16861 ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa ttgctacagc acccattacg
    16921 atagcagatg ttggtgtaat taagtttact aaaccagatg cagattgata agcagtgatt
    16981 actaaatcct taccgacgcc ggcaaattca cccataggtc ccatgattcc cattgttgca
    17041 gcagctaagc cggaagttga aggaatcaag aatgacatag ggatatagaa aataaatgta
    17101 ataatgatga atacacttga agataatgaa cttaatcctt gttcgcccca atgtaagatg
    17161 gtgtctgtga ttaatccatt gttcataacc acttggatac cacgagcaac agctactacg
    17221 attgctacac cgatcaaatc agaagcacca gctaaaaatt cagagacaaa ttcagactct
    17281 ttcatgccat aagtgattgc tactacgatg gacattacta agaataacat agtaatttct
    17341 gggaagtacc agtcacctaa tggaagcatg cttttaccta gaaccgtacc tagtaccgga
    17401 aggtttgtta accagtttgt caatgattca aaaatcgtga aattcgcatt caaggttgtc
    17461 caaggaatca aactgatgat catgatacca aaagtaataa agaacaatac attaacatgt
    17521 ttttgttttt tcgtgattac ttcttgacga ccgattgctt caatatcgaa atgcttcata
    17581 tcttctttac gttggttgta tactaatgat tttgttggat ctttttcgat ctttgatgca
    17641 taacgataca catatacaat agaaatagct aacaaaataa cgaacatcaa gaatcgaaga
    17701 ccaatacctt cacccatcga aatcccaaca gcttgtgaag ctacgcctgt tgcaaaagga
    17761 tttaccgttg aagctagaca gcctacttga gaccctacta atacgattgc tacagctact
    17821 actgtatcaa agcctacagc catcatgact gggatcagta aagcatagaa tggaatcgtt
    17881 tcttctgcca ttccgtaagt agagccgcct aatgcaaata acagcatcaa tactggaata
    17941 agcatttttt cttttccttt gaatttcttc acaactgaag caatcccggc atctagtgcc
    18001 cctgttttgt tgataatacc taaaaatcca ccaacaacca aaatgaagaa agagattggg
    18061 attgcagcag gtgtttcttt agttagctcc cccgctggta cacctagcat cccattgatt
    18121 ggcgccatga aaatatccca cagcccttga ggattttgtt gtactcgttc atatgatcca
    18181 gcgacgatgt tgcctgcatc gtctacacta tagtgcccag ctggtataaa ccaagtaagt
    18241 acagcgatta ctgcaataat aataaataag accgtatacg ctgaaggcat ttgaaatttt
    18301 ttcttttttg tttgttccat cttcttcttc ctttcttttc cgttagagac aacaattttg
    18361 gctctaatgt tattttatca aaaagagtat cagaacaaaa ataataacac gcattttgta
    18421 aaacttttgt tcaaaaagtt cgtttttccc tattccctcc tcttttttca ggtttttaaa
    18481 gctttcttct ttttcattgt agcgtttcct tttttttata aacaaacatt ttcgtctttt
    18541 aaaaggagaa gaatttcaca tttgcaaaga attgattgat ttttatgcat aaatcaaaaa
    18601 aatactcaaa acaaacaaat aaaacgttta cattattgca tgtcttgtat ttttttccaa
    18661 aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct gtatgacgtt
    18721 gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc agcactaaaa
    18781 agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca atcagtacat
    18841 agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac attaagtaaa
    18901 gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg atggttcctg
    18961 taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta aagaaataat
    19021 aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct accaaattat
    19081 ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct gttgcaactc
    19141 cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta aaatcgatct
    19201 tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact aatgcgatcg
    19261 cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt tgaaatcctc
    19321 ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa aaaactctat
    19381 tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa gctctatctt
    19441 ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt tttttgcaaa
    19501 ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa gacgaacctt
    19561 atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca acatctaaca
    19621 cttataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt cttttttgtt
    19681 cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt atttttcacg
    19741 ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt gatgattacg
    19801 tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca ctgataaaat
    19861 ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg tcaaaggtgc
    19921 aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat ccacttcatg
    19981 ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca agatccccga
    20041 agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg gcaaaagatc
    20101 cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt taggtttgta
    20161 caataaatag taaccgtatt cttcttgata aattgggact tcatccactt ccacagtgtc
    20221 atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt ttacacgtcc
    20281 tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg caattacttt
    20341 ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa tagatctggt
    20401 gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc ttctaatgat
    20461 ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg ccgacctggt
    20521 ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg taccgctcct
    20581 gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg tttataagcg
    20641 atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc ttgcgcatat
    20701 cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct ttccgtttca
    20761 aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc tttcactaaa
    20821 cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt actgatccct
    20881 tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat gttcggtatc
    20941 tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg caatgatctg
    21001 cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac ttttagaagg
    21061 tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac gctcttcgat
    21121 aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt ccactttttg
    21181 agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga tttttttgac
    21241 ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca taggttcttt
    21301 tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg catatttata
    21361 tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt cccataattc
    21421 ttcatcgtct gtgccgaatt cttggacagg aagcaatgtc ttgcttttga ttgccatcaa
    21481 tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt tcattgtatg
    21541 gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata tatcgatttc
    21601 catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat ctaatttaat
    21661 attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca ttttccaatg
    21721 taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc ctgtaattca
    21781 tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga aatgtcatgg
    21841 atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat gttggttgaa
    21901 ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt atcaatctcc
    21961 ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc catagcaatt
    22021 ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga ctaagccctt
    22081 ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt ataaatctga
    22141 gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc agctccattt
    22201 tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc tttgtaaatc
    22261 ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga aagacctttc
    22321 ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa tggacgtgct
    22381 tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat gatccgctct
    22441 ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc tcccaatttc
    22501 aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa gattgcacga
    22561 tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc tacttctgtc
    22621 agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg ttgcatcgga
    22681 tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag acttgagatc
    22741 atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag aaaacggatg
    22801 actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag aaaatgcaag
    22861 tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc ccgttcaata
    22921 ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct catgcctctc
    22981 tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag caaagaaaaa
    23041 gccgtctaga cactcagacg gactggttt
//
LOCUS       DAFGFZ010000042        22742 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000042 DAFGFZ010000000
VERSION     DAFGFZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.042"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            2..370
                     /locus_tag="LR726_001366"
     CDS             2..370
                     /locus_tag="LR726_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL6216467.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="LR726_001367"
     CDS             550..1491
                     /locus_tag="LR726_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL6216468.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="LR726_001368"
     CDS             1560..2291
                     /locus_tag="LR726_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL6216469.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="LR726_001369"
     CDS             2435..4615
                     /locus_tag="LR726_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL6216470.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="LR726_001370"
     CDS             4635..5117
                     /locus_tag="LR726_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL6216471.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="LR726_001371"
     CDS             5133..5894
                     /locus_tag="LR726_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL6216472.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /locus_tag="LR726_001372"
     CDS             6025..7359
                     /locus_tag="LR726_001372"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL6216473.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="LR726_001373"
     CDS             7666..8151
                     /locus_tag="LR726_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL6216474.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="LR726_001374"
     CDS             8144..8827
                     /locus_tag="LR726_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL6216475.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="LR726_001375"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="LR726_001375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL6216476.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="LR726_001376"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="LR726_001376"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL6216477.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="LR726_001377"
     CDS             12443..13072
                     /locus_tag="LR726_001377"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL6216478.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="LR726_001378"
     CDS             13124..13456
                     /locus_tag="LR726_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL6216479.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="LR726_001379"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="LR726_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL6216480.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="LR726_001380"
     CDS             14282..15655
                     /locus_tag="LR726_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL6216481.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="LR726_001381"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="LR726_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL6216482.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="LR726_001382"
     CDS             16710..17150
                     /locus_tag="LR726_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL6216483.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17294..17995
                     /locus_tag="LR726_001383"
     CDS             17294..17995
                     /locus_tag="LR726_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL6216484.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17992..19113
                     /locus_tag="LR726_001384"
     CDS             17992..19113
                     /locus_tag="LR726_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL6216485.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19128..20360
                     /gene="pepT"
                     /locus_tag="LR726_001385"
     CDS             19128..20360
                     /gene="pepT"
                     /locus_tag="LR726_001385"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL6216486.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20492..21400
                     /locus_tag="LR726_001386"
     CDS             20492..21400
                     /locus_tag="LR726_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL6216487.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21435..21707
                     /locus_tag="LR726_001387"
     CDS             21435..21707
                     /locus_tag="LR726_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL6216488.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21718..>22742
                     /locus_tag="LR726_001388"
     CDS             21718..>22742
                     /locus_tag="LR726_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL6216489.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKK"
BASE COUNT         7661 a         3972 c         4998 g         6111 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag
    17281 aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt
    17341 tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc
    17401 attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta
    17461 tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct
    17521 tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg
    17581 tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag
    17641 cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct
    17701 gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata
    17761 tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat
    17821 gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga
    17881 gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg
    17941 tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc
    18001 agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa
    18061 ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg
    18121 acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg
    18181 attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa
    18241 gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac
    18301 atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg
    18361 gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct
    18421 gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt
    18481 gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt
    18541 gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag
    18601 gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct
    18661 tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta
    18721 ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa
    18781 gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg
    18841 aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa
    18901 agagcagatg tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc
    18961 gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt
    19021 atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct
    19081 gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc
    19141 ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa
    19201 cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag
    19261 agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac
    19321 caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag
    19381 attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga
    19441 tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa
    19501 attatgaagg ccacacattg atcacaacag atggcagtac attactggga gcagatgaca
    19561 aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc
    19621 ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata
    19681 aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag
    19741 gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa
    19801 atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt
    19861 ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct
    19921 tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg
    19981 atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt
    20041 tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca
    20101 tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg
    20161 agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct
    20221 cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat
    20281 tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag
    20341 aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa
    20401 tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc
    20461 tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa
    20521 aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga
    20581 aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga
    20641 attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg
    20701 cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt
    20761 acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga
    20821 agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa
    20881 tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag cagtttggat
    20941 cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt
    21001 attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg
    21061 aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga
    21121 cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat
    21181 tttccgattt attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg
    21241 cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac
    21301 tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg
    21361 attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag
    21421 ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg
    21481 tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct
    21541 ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat
    21601 tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg
    21661 ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg
    21721 tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt
    21781 gccattattt attatttaat caatcaatca tttgcagata tctttgccga attgctgtct
    21841 accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa
    21901 gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg
    21961 ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg
    22021 atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg
    22081 acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg
    22141 gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct
    22201 ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat
    22261 gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa
    22321 tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc
    22381 caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg
    22441 tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc
    22501 atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt
    22561 ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca
    22621 gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt
    22681 gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa
    22741 ga
//
LOCUS       DAFGFZ010000043        22639 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000043 DAFGFZ010000000
VERSION     DAFGFZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.043"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            133..3660
                     /locus_tag="LR726_001389"
     CDS             133..3660
                     /locus_tag="LR726_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL6216490.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="LR726_001390"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="LR726_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL6216491.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="LR726_001391"
     CDS             7448..7789
                     /locus_tag="LR726_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6216492.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="LR726_001392"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="LR726_001392"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL6216493.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="LR726_001393"
     CDS             9146..11566
                     /locus_tag="LR726_001393"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL6216494.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LR726_001394"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LR726_001394"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL6216495.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="LR726_001395"
     CDS             13000..13737
                     /locus_tag="LR726_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL6216496.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="LR726_001396"
     CDS             13751..14566
                     /locus_tag="LR726_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL6216497.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="LR726_001397"
     CDS             14579..15226
                     /locus_tag="LR726_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL6216498.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="LR726_001398"
     CDS             15242..15898
                     /locus_tag="LR726_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL6216499.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="LR726_001399"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="LR726_001399"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL6216500.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="LR726_001400"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="LR726_001400"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL6216501.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="LR726_001401"
     CDS             18240..18758
                     /locus_tag="LR726_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL6216502.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="LR726_001402"
     CDS             18865..19365
                     /locus_tag="LR726_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL6216503.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="LR726_001403"
     CDS             complement(19645..20565)
                     /locus_tag="LR726_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL6216504.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="LR726_001404"
     CDS             20730..21743
                     /locus_tag="LR726_001404"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL6216505.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="LR726_001405"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="LR726_001405"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL6216506.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7536 a         3949 c         4821 g         6333 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaattt
//
LOCUS       DAFGFZ010000044        22497 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000044 DAFGFZ010000000
VERSION     DAFGFZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.044"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(219..2111)
                     /locus_tag="LR726_001406"
     CDS             complement(219..2111)
                     /locus_tag="LR726_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216507.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2108..3835)
                     /locus_tag="LR726_001407"
     CDS             complement(2108..3835)
                     /locus_tag="LR726_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216508.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3851..4501)
                     /locus_tag="LR726_001408"
     CDS             complement(3851..4501)
                     /locus_tag="LR726_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL6216509.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4705..5001
                     /locus_tag="LR726_001409"
     CDS             4705..5001
                     /locus_tag="LR726_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL6216510.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSLTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5173..5793
                     /locus_tag="LR726_001410"
     CDS             5173..5793
                     /locus_tag="LR726_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL6216511.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5885..7051)
                     /locus_tag="LR726_001411"
     CDS             complement(5885..7051)
                     /locus_tag="LR726_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL6216512.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7063..7416)
                     /locus_tag="LR726_001412"
     CDS             complement(7063..7416)
                     /locus_tag="LR726_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL6216513.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7544..8566
                     /locus_tag="LR726_001413"
     CDS             7544..8566
                     /locus_tag="LR726_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL6216514.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8585..8746
                     /locus_tag="LR726_001414"
     CDS             8585..8746
                     /locus_tag="LR726_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216515.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9005..9439)
                     /locus_tag="LR726_001415"
     CDS             complement(9005..9439)
                     /locus_tag="LR726_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216516.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9549..11408)
                     /locus_tag="LR726_001416"
     CDS             complement(9549..11408)
                     /locus_tag="LR726_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL6216517.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11526..12686)
                     /locus_tag="LR726_001417"
     CDS             complement(11526..12686)
                     /locus_tag="LR726_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBL6216518.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12993..14417)
                     /gene="araA"
                     /locus_tag="LR726_001418"
     CDS             complement(12993..14417)
                     /gene="araA"
                     /locus_tag="LR726_001418"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL6216519.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14433..15131)
                     /locus_tag="LR726_001419"
     CDS             complement(14433..15131)
                     /locus_tag="LR726_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL6216520.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15088..16749)
                     /locus_tag="LR726_001420"
     CDS             complement(15088..16749)
                     /locus_tag="LR726_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL6216521.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVSGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16967..18367
                     /locus_tag="LR726_001421"
     CDS             16967..18367
                     /locus_tag="LR726_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL6216522.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18619..19698)
                     /locus_tag="LR726_001422"
     CDS             complement(18619..19698)
                     /locus_tag="LR726_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6216523.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19784..20743)
                     /locus_tag="LR726_001423"
     CDS             complement(19784..20743)
                     /locus_tag="LR726_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL6216524.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20755..22266)
                     /locus_tag="LR726_001424"
     CDS             complement(20755..22266)
                     /locus_tag="LR726_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL6216525.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22288..>22497)
                     /locus_tag="LR726_001425"
     CDS             complement(22288..>22497)
                     /locus_tag="LR726_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216526.1"
                     /translation="ISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIFLF
                     PPFIFIFAGGVWKLVSIILTIGNER"
BASE COUNT         6408 a         4765 c         3978 g         7346 t
ORIGIN      
        1 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg
       61 agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt ttgagctaat
      121 accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga cacgactttt
      181 gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc ttcttcagaa
      241 aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt atcgtggttt
      301 cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc gcgtattgta
      361 gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt catcgctttt
      421 tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag gatcaggaca
      481 tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc ttgcgaaata
      541 ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg aacaaaatcg
      601 tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc gtcgttccca
      661 tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag taccattgaa
      721 aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc gtaacgaata
      781 ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt cttccctgca
      841 ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt aaaatcagtc
      901 attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac tttgtatggt
      961 gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc atccaacact
     1021 tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa acttgcaagt
     1081 tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac atcccctaat
     1141 gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac atagcctaga
     1201 tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc tttccaacca
     1261 gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc agtatgattg
     1321 aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt caataaacct
     1381 aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat catcacgatg
     1441 atcaagctta aagggatcat caataacgcg actaacgtca attgccagct gattgaaagc
     1501 atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt caaattttgc
     1561 tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat atccccattt
     1621 gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa atcatgacgt
     1681 aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa aaagttgaat
     1741 acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat tttatcaaag
     1801 tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc agccattaca
     1861 cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat ttggaagatc
     1921 acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt agacatatag
     1981 ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt tacaccgaga
     2041 tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg acgtgtgttt
     2101 gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct tgataagttt
     2161 cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct tcatctagca
     2221 caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa acggtgatac
     2281 tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg aaatccaacg
     2341 ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc cgtgcaattg
     2401 ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca tggctatcta
     2461 agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat gctttccaaa
     2521 tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc gttccagaga
     2581 acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat tgagtcatgt
     2641 cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat aatcgaggga
     2701 tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg acgatctcgc
     2761 ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca tgatatcgat
     2821 aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact gttttaggat
     2881 tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg tcagctgaag
     2941 cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg atcaagatct
     3001 gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata tctgctatgt
     3061 agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat gtcatcattg
     3121 gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa tctttattcg
     3181 ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc actctgaccc
     3241 ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc atacttttga
     3301 ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact gggatgacaa
     3361 ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc gcgccaatca
     3421 acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt tggatttgag
     3481 tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca aacgcatctt
     3541 ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc agtttctgtg
     3601 attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc aaagaaaagc
     3661 cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct acccctttat
     3721 caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg atcaccatga
     3781 aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt agcatctagt
     3841 tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa ttgaattcac
     3901 tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac gaatgcgcaa
     3961 ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca tgatcatttt
     4021 ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct tgtttgtgtt
     4081 ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact tttcttgagg
     4141 cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg ccttccaaaa
     4201 tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca ccatcggatt
     4261 ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca aagtaataaa
     4321 aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg atatccgcta
     4381 atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac tcgattctcg
     4441 cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc tgtgtcggca
     4501 tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt cattataaca
     4561 cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc gatacattaa
     4621 aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt aaaatagcca
     4681 catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga cttcaataaa
     4741 ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga ttacacaaaa
     4801 aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca attaaaaaat
     4861 ctagatccaa agaaacttta ttatattcta agccttacag gcaggcgatc agaagcatta
     4921 gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg gatggaagct
     4981 cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg tgacaaaaaa
     5041 tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt caaccttatc
     5101 aatatgactt gatagccttt tctttttctc atagttataa caatcatagc aataaaagga
     5161 gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac tgcagaagaa
     5221 tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa tcatccagat
     5281 gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga tgaagaatta
     5341 ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc agaaagtcaa
     5401 caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc tgataaaatc
     5461 gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc ttggttcgat
     5521 accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga aggattagtg
     5581 acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg acaagacttt
     5641 gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt agatcagcta
     5701 ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca aaatgcatta
     5761 gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca tacaaaagag
     5821 acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca tctgtctctt
     5881 tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag attgttgtgt
     5941 accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga atccccaaga
     6001 tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa atcgttactg
     6061 gatggattgc taactgattc cctagtacta atggagagat caagcgtcct tctattgtct
     6121 gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct acaatggcta
     6181 aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg ttcaaaaaac
     6241 ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa ccaatcataa
     6301 acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa gaaacttgac
     6361 tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt aaaaatttga
     6421 caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata aacggcatcg
     6481 tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga ctctgaactg
     6541 tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta tcacctgctg
     6601 tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg gttaatttat
     6661 tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt tgttcctgaa
     6721 tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa acaaacagcc
     6781 caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact accggattca
     6841 tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct aaaaattgcc
     6901 agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc aatattaaca
     6961 atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa aaccacgata
     7021 ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat aaaaaatttg
     7081 gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat ttgcaatttg
     7141 tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct ctaaattccc
     7201 attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag caattgttcc
     7261 gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg aatattttcg
     7321 taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct tgtttattgc
     7381 ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt taattgcttt
     7441 caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg tgatagaata
     7501 aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg aaaagataat
     7561 tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc ttgatacaga
     7621 aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta cctatgtagc
     7681 aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag gtggtgccgg
     7741 tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg ctcctttgtg
     7801 ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc ataaaggtga
     7861 agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt caatattcca
     7921 tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact atacggatct
     7981 gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt atacttatga
     8041 tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc caggaacagg
     8101 ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg gtgaattaga
     8161 cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag aattacagaa
     8221 aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta tccgcatttc
     8281 aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg ctgtattcgc
     8341 agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag aaggggattt
     8401 tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca atcagaacac
     8461 cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat tactgaaaaa
     8521 agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag aaaaggagaa
     8581 tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata ttcctttctt
     8641 tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac tcaaagccga
     8701 tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag ctttccatac
     8761 tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct tttctacaaa
     8821 taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg attttgagta
     8881 agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt gatcactcta
     8941 tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt atcaaagatt
     9001 tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc aattggtctg
     9061 tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc cctgattcaa
     9121 gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa gctttttcaa
     9181 aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact ttcttaagca
     9241 ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca agagtcgatt
     9301 tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca tatttcggat
     9361 gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac tgcacataat
     9421 agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta ttatttattc
     9481 tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt ctatacagct
     9541 cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt ccctgcgaca
     9601 atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg attgaattgc
     9661 gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc cggaagttcc
     9721 tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt ctgttccatt
     9781 agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg cttttctaat
     9841 tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt gtcatttttt
     9901 gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac aactaaagtc
     9961 cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct gctataggaa
    10021 atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt ttttgcagga
    10081 tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt gactaaatga
    10141 atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta agctgactca
    10201 tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg ctcgctgatg
    10261 aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga agtattgtat
    10321 ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat agaaggaagc
    10381 tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt aaataacgcc
    10441 atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc atagttttga
    10501 gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc caatggctca
    10561 ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact catcacgaac
    10621 acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag cttctggtat
    10681 ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc cattggttct
    10741 accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt cattttctgg
    10801 ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt atcgggattg
    10861 ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt taatcggatc
    10921 aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa caatcctatc
    10981 acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat atctttcagc
    11041 aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg ccgataactc
    11101 attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt aagggaacct
    11161 aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa gacgagacag
    11221 gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag gtctacggat
    11281 agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa aaagatactg
    11341 atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct gcttatatat
    11401 cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc tcttatttta
    11461 gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc aaatattctc
    11521 tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc agaaattttt
    11581 tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat gtcaatcaat
    11641 atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag ctcgttctca
    11701 ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa tatagtgaat
    11761 cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc tctaaccgta
    11821 ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc tgccaaagct
    11881 tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc taagaactct
    11941 tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc ttgcataaca
    12001 gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc ttttttcatt
    12061 ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg cagcatgact
    12121 acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac ataatccaac
    12181 tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca acttagcgct
    12241 acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg caattctcgt
    12301 cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc tgttagtgtt
    12361 tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca gcacaaaaca
    12421 agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt cccgttttca
    12481 gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa
    12541 tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc ttcggctggc
    12601 aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg ttctggaata
    12661 gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt atctcgaaat
    12721 ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga gcagttagag
    12781 cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa gccttatttt
    12841 cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta gctgacattg
    12901 ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc
    12961 ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt ttccaactca
    13021 actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac aacggtatga
    13081 tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg tttagttttc
    13141 cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa ggcttctact
    13201 tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac taccccttct
    13261 cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat tgataaagga
    13321 gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat catatgggat
    13381 tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat gaatcctgta
    13441 tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct
    13501 tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc aggtagttgt
    13561 ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag
    13621 aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc ccatgtcttt
    13681 tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa taattgatcg
    13741 acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc aaaataatca
    13801 acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac acccacgttt
    13861 ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata ggctactgca
    13921 acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta gccaacgacg
    13981 attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc accatgagcg
    14041 gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct ttcgttatat
    14101 tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat ccacattttt
    14161 gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg ataattgacc
    14221 tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag gcggattgga
    14281 taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg tttctttact
    14341 tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca aaattctttt
    14401 tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta atacgcattt
    14461 tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc ttctgaaaat
    14521 gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac gactgcgttc
    14581 attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc
    14641 cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat aacttttcct
    14701 gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc
    14761 gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt
    14821 gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg aggaaatgaa
    14881 cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc ttcaatgata
    14941 tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata actgactccg
    15001 ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt tccccatgta
    15061 tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc ttctttcagt
    15121 tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg ttttttcaat
    15181 gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt cttctgctga
    15241 aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga agttttctaa
    15301 agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc acgctccacc
    15361 ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta gaatccgctg
    15421 tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa tttggacatt
    15481 ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg ctaaatggat
    15541 ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata atggccttcc
    15601 ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta atcccccgca
    15661 atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat ctctgcttat
    15721 ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat tgatatccga
    15781 agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag tgaccatgtc
    15841 gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga aggcggaagt
    15901 accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca taccggttcc
    15961 tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg tagggtcgat
    16021 cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgatacta gaactttagg
    16081 caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac caaaaatatt
    16141 tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc ctgatgcatc
    16201 tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg caagcgttgt
    16261 caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat acaaatgagc
    16321 gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc gttctgcttc
    16381 ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct tgctatcaaa
    16441 tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg taagattctc
    16501 gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca
    16561 aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc actcataact
    16621 accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc tagttgatcc
    16681 aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt gcgcatttac
    16741 ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc gtctttttag
    16801 tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt tatttacaag
    16861 aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa ttgcttacaa
    16921 aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga aaaaagaaaa
    16981 aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc tatttggata
    17041 tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg gtttggcagg
    17101 taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg
    17161 ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc ttctttctgc
    17221 acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg gttcttattt
    17281 tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg ccctagttcc
    17341 tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag ggatcaatca
    17401 agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc taaaagtgtt
    17461 gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg cgctgatttt
    17521 atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat ctggccgctt
    17581 ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg aacaggaatt
    17641 cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt ggcatacttt
    17701 gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag tattccagca
    17761 atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa aagcgactgg
    17821 gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt tagttgctgg
    17881 ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg
    17941 cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg tactggatgc
    18001 tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat tttattcttg
    18061 cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag ctgtccgcgg
    18121 gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag ttggcttgct
    18181 ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt ttgggatcat
    18241 ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt
    18301 agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt ctatccatca
    18361 ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga accgcttagt
    18421 ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa tattgtgact
    18481 tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa acgttcagcc
    18541 ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga gcaataagag
    18601 gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt caactagtac
    18661 aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga cgatccaccg
    18721 tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt ttttattccc
    18781 taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg gaatatgata
    18841 gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat agcaaatgat
    18901 gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg
    18961 tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat actgttcaca
    19021 cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa
    19081 caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac cgactttcac
    19141 atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga tcatcaccat
    19201 cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat actgattact
    19261 tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct tcaaacactc
    19321 tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc cagattctcg
    19381 taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga gatatgtggt
    19441 gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat agctgtcatc
    19501 tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca acacagcgat
    19561 tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga attctgaggg
    19621 aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct cttctgcaat
    19681 atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata tatcataacg
    19741 agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag gcatacggaa
    19801 caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc gcgttcgcgt
    19861 gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga gcatgataga
    19921 tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt
    19981 gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat gattctttta
    20041 ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt tcatctgttg
    20101 cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct ggaccttcat
    20161 ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg atctgcttcc
    20221 cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc ccagtaattt
    20281 caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa gttgttccgt
    20341 ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa tctgtattca
    20401 ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat tcatcacgta
    20461 tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata taatggactt
    20521 caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat acaatcaggc
    20581 ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc acttggtagc
    20641 ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc caaggatctg
    20701 cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat ttcctcacat
    20761 tttctttatt ctaattacat tccaagagta tttttctatc cctacattta attcgtcatg
    20821 tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt cacgtgtatt
    20881 tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta gcaaattgaa
    20941 atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga aaatagtcag
    21001 ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta tcttttcaca
    21061 atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat atttcgataa
    21121 gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat ctgtcagtat
    21181 taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat ctgcgtgtcg
    21241 taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa agtaacacca
    21301 atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg catgtacatt
    21361 ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt ttacataatc
    21421 acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg caaggtatgt
    21481 cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat catacttatc
    21541 aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta atgcgatacg
    21601 atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat
    21661 tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac catattcata
    21721 agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac accataattt
    21781 aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga aggttccttt
    21841 tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg ttcctaaatt
    21901 caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt cgtggagtcc
    21961 aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc gttgttcttt
    22021 cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag gatagcgaat
    22081 gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc cgtcaccatc
    22141 agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca tatgctcaac
    22201 aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc tgatttttac
    22261 tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca agataataga
    22321 tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag
    22381 cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat caaaaaatac
    22441 tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt cagaaat
//
LOCUS       DAFGFZ010000045        22410 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000045 DAFGFZ010000000
VERSION     DAFGFZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.045"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            6..305
                     /locus_tag="LR726_001426"
     CDS             6..305
                     /locus_tag="LR726_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6216527.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LR726_001427"
     CDS             378..1739
                     /locus_tag="LR726_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL6216528.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LR726_001428"
     CDS             1761..2021
                     /locus_tag="LR726_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216529.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LR726_001429"
     CDS             2035..2787
                     /locus_tag="LR726_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL6216530.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LR726_001430"
     CDS             complement(2881..3486)
                     /locus_tag="LR726_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6216531.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LR726_001431"
     CDS             complement(3539..4513)
                     /locus_tag="LR726_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL6216532.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LR726_001432"
     CDS             complement(4891..5610)
                     /locus_tag="LR726_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6216533.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LR726_001433"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LR726_001433"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL6216534.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LR726_001434"
     CDS             7161..8558
                     /locus_tag="LR726_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL6216535.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LR726_001435"
     CDS             8639..9079
                     /locus_tag="LR726_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL6216536.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LR726_001436"
     CDS             9227..9553
                     /locus_tag="LR726_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL6216537.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LR726_001437"
     CDS             9554..10303
                     /locus_tag="LR726_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216538.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LR726_001438"
     CDS             complement(10383..10838)
                     /locus_tag="LR726_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL6216539.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LR726_001439"
     CDS             complement(11265..11540)
                     /locus_tag="LR726_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216540.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LR726_001440"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LR726_001440"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL6216541.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LR726_001441"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LR726_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL6216542.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LR726_001442"
     CDS             14770..17436
                     /locus_tag="LR726_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL6216543.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LR726_001443"
     CDS             17800..18552
                     /locus_tag="LR726_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL6216544.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LR726_001444"
     CDS             complement(18574..19728)
                     /locus_tag="LR726_001444"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL6216545.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LR726_001445"
     CDS             19913..22354
                     /locus_tag="LR726_001445"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL6216546.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHIGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7199 a         3932 c         4669 g         6610 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatagga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca
//
LOCUS       DAFGFZ010000046        22031 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000046 DAFGFZ010000000
VERSION     DAFGFZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.046"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            287..1087
                     /locus_tag="LR726_001446"
     CDS             287..1087
                     /locus_tag="LR726_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216547.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            1175..1981
                     /locus_tag="LR726_001447"
     CDS             1175..1981
                     /locus_tag="LR726_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216548.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            2212..3081
                     /locus_tag="LR726_001448"
     CDS             2212..3081
                     /locus_tag="LR726_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL6216549.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            3101..3427
                     /locus_tag="LR726_001449"
     CDS             3101..3427
                     /locus_tag="LR726_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216550.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            3461..4723
                     /locus_tag="LR726_001450"
     CDS             3461..4723
                     /locus_tag="LR726_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216551.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            4803..6179
                     /locus_tag="LR726_001451"
     CDS             4803..6179
                     /locus_tag="LR726_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL6216552.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            6176..6805
                     /locus_tag="LR726_001452"
     CDS             6176..6805
                     /locus_tag="LR726_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216553.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            6817..7275
                     /locus_tag="LR726_001453"
     CDS             6817..7275
                     /locus_tag="LR726_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216554.1"
                     /translation="MLTILKLYREGFNEKVSIGESIEYRGDKYIIIRILKINKHYFSK
                     SISLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYEV
                     VSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVLEGEKK"
     gene            7272..7751
                     /locus_tag="LR726_001454"
     CDS             7272..7751
                     /locus_tag="LR726_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216555.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
     gene            7890..8429
                     /locus_tag="LR726_001455"
     CDS             7890..8429
                     /locus_tag="LR726_001455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216556.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFV"
     gene            9080..9133
                     /locus_tag="LR726_001456"
                     /pseudo
     CDS             9080..9133
                     /locus_tag="LR726_001456"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9137..9736
                     /locus_tag="LR726_001457"
     CDS             9137..9736
                     /locus_tag="LR726_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL6216557.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            9752..10228
                     /locus_tag="LR726_001458"
     CDS             9752..10228
                     /locus_tag="LR726_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216558.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            10245..11147
                     /locus_tag="LR726_001459"
     CDS             10245..11147
                     /locus_tag="LR726_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216559.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            11220..11411
                     /locus_tag="LR726_001460"
     CDS             11220..11411
                     /locus_tag="LR726_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216560.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            11576..12163
                     /locus_tag="LR726_001461"
     CDS             11576..12163
                     /locus_tag="LR726_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216561.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            12163..13062
                     /locus_tag="LR726_001462"
     CDS             12163..13062
                     /locus_tag="LR726_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBL6216562.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            13062..13976
                     /locus_tag="LR726_001463"
     CDS             13062..13976
                     /locus_tag="LR726_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216563.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            14028..14243
                     /locus_tag="LR726_001464"
     CDS             14028..14243
                     /locus_tag="LR726_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216564.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            14433..14687
                     /locus_tag="LR726_001465"
     CDS             14433..14687
                     /locus_tag="LR726_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216565.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            14866..15909
                     /locus_tag="LR726_001466"
     CDS             14866..15909
                     /locus_tag="LR726_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL6216566.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            16082..16306
                     /locus_tag="LR726_001467"
     CDS             16082..16306
                     /locus_tag="LR726_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216567.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            16309..16566
                     /locus_tag="LR726_001468"
     CDS             16309..16566
                     /locus_tag="LR726_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216568.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            16668..17021
                     /locus_tag="LR726_001469"
     CDS             16668..17021
                     /locus_tag="LR726_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216569.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            17074..17868
                     /locus_tag="LR726_001470"
     CDS             17074..17868
                     /locus_tag="LR726_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216570.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            17897..19015
                     /locus_tag="LR726_001471"
     CDS             17897..19015
                     /locus_tag="LR726_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216571.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            19048..19368
                     /locus_tag="LR726_001472"
     CDS             19048..19368
                     /locus_tag="LR726_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216572.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            19623..20516
                     /locus_tag="LR726_001473"
     CDS             19623..20516
                     /locus_tag="LR726_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216573.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            20536..>22031
                     /locus_tag="LR726_001474"
     CDS             20536..>22031
                     /locus_tag="LR726_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216574.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYT"
BASE COUNT         8499 a         2815 c         4523 g         6194 t
ORIGIN      
        1 tgatctcttt tttattttga atagagctag attcactttt aaataaaatc aaaactgatc
       61 ttaattctaa agaaaaagtc aagaaaaaaa tacaacaaat ttttttatca gaatatttat
      121 cagaatatta ttagaaaaga taaactcaaa agcttaattt aatagggtta ttggttgtat
      181 ttagaaataa tgtatggaaa agctataaag aaatagataa gatgtagaaa atcttatttg
      241 tgaatgaaaa acaagtcatc gtaaaatatt aataggaggt gtagtaatgg tgggaatatc
      301 aaaaacagct ttagactcat tagctaaact atttagtagc aggtttgata gtgcagaatt
      361 tgttgaatat ctaagccaaa acgaaagtgg aaaaactctg atcgaaacaa tgaaaaaaga
      421 ctatttgaat gaaaatgact ttccgacatg gacgaatatt gaagatgcta catgggatgt
      481 agtgtgtacg atatattctc cttgggtaac gattgctgat tttgttttag ataaaattgg
      541 aggggataca taccctgatg ccaaatctct tgctacaagt gactttaaaa cgttctatgg
      601 ttatctaata ggtctaatag gcaatggaat agatttgaaa gttacgccaa aacaaacacc
      661 tgaagaaatt gaaaaagaaa aaggagaggt tcaaatggct tatgcaaatg acagagaaaa
      721 agtaaaatta ttggaaggta aattatctga agaagaactt ggaaaaatgg tgcaagaaat
      781 caatgaagac agcaactatt ttcctgaatt gaaggtatat caaaatgacg aagatttctt
      841 taatgaaatg tttagtaata gtccatggga agcagtgagt aaaacaaatt taggagaatg
      901 gaactttcaa gatgatctag ttgcatttag caatgatggg tatttacgat catattcaga
      961 aagtgagtat agagctgaat taaaaacgta tgaaacggat attctgatga gctatttaaa
     1021 tctattagag agtggtcagg gagagttaag agataaagtt gaagaactat taaaagaaga
     1081 gctataggga ttatttcaac ataaacacaa gcagaaaact accaaacaaa acgtaccatg
     1141 caaaaagtcg gttaagccgg ctttttgctc tattatgaga ggagatcaaa ccatgagtgc
     1201 aacaaccgat atggctttag aagcagtaat aaaagataat acaggaaatg tcgaaaatgt
     1261 agaagacttt agcgatattg caatcaatct ttcagcatgg atttcagctg taggagaaga
     1321 aaatatcgaa tttgagatca agaatatgga tgacttaaaa gcaaaatttc cttttatcgc
     1381 ttcgaaacta agtcaatttg ccgaggataa atcgtggaat gaattagtta gtttttccga
     1441 aaaaatttat gatattacgc aagaagatga gtgtgtttca tgggaaatga ataatcaata
     1501 caagaaaatt gatgctttcg tcaaatcacg tatagaagga acagcttttg agtttgaagg
     1561 tatagatacc gcagaaaatt ggttaaataa agaatccttt attaaaagta tagcggatga
     1621 tcttgaaaaa caatggtcta aggaagacta tgcagagtac ctaaaaacag aaagagatac
     1681 agaggtgaaa gaaaaactca ctgaggtatg tttggatgaa catattgatc ccaatatcgt
     1741 aaaacaagaa gactatgaag tggttattga taaagttcct actgattttg aggaagttgt
     1801 aaagtatata gttgaagaag cattatcgca atactcttca gatgaggaag tggacgaaga
     1861 aactgttcgt accagagttt ggcaactggt ttttgatgac aaagattata aatatttgag
     1921 tgatcaaaac tcggttaatt ggagtgtacg attattaatt gatcaaaatg atattgaata
     1981 aaatagagct gaaaagctct atttttttga taaaaaaact agctatttta ttaaaaataa
     2041 tttggttcaa aatttatttt tttaacttga taattttttg tcatcctttt ttaaattggc
     2101 tatatatcaa ggtttatagt gggaacaaat aaaaatacat ttgacaaaat ttacaatata
     2161 gtttattata tatttatctt attatgataa cacaataaaa ggaatgatgg tatgaaaaag
     2221 aagaagttta gtcgatcgtt gttaggaatt ttggttttag ttgcggttgg tcttagtggc
     2281 aagggttact ttcagaataa cagcactcag gaacatgatt ttcagatcaa aagtagtgtt
     2341 caggtaggta ataaagcaga cagtgaatca aaagaatttt tagggatacg ttatgatcca
     2401 aacaaatatc caaataatat cgtaaacatt aatgatggga aatatacact gacaaaagaa
     2461 gaccatgaag cattatcaga aaaaggcagt caaacctttt gggtaaatta tgcagattta
     2521 gatcaacttg gaagagcagg aaatgttgaa gcgttgatca cctatgatgc tgtaaattct
     2581 cactcatcta aaaatgtgga acgtccatct ttcaaaagtt ctgtccatat tgcaggagaa
     2641 tacaaggatg gagtatttga tcaagagaga gatacttggg tcggcaaaga ttcaaacaat
     2701 gaaatcactc agctagaagg ctataaaggt tatctctata acaaaagtca ttcattagca
     2761 tggtcgctag gaggagatat ggaggtacac aatttaacac ttggaacgag agcgcaaaat
     2821 gttgggaaaa atgatcagaa tggagggatg gcttactcag aaacgattgt acgtgatgca
     2881 atctataaaa ataagaatga aaaagtattg tataaagttg atccaattta taaagggaat
     2941 gaattggttc ctcgtggttc tcatgtcgaa gctttttcag ttaacgataa tggaaaaacg
     3001 gtaaacctta atgtttatgt atttaacaat caaaaaggaa tcaacatcaa ctatcaaaat
     3061 ggtacttggg taagagatta gaaattgaga aggtgaagaa ttggaaacaa taagcatcaa
     3121 tggattagat aaatcagttg caaatcgaat aaaaaaagga aatggctact atcttctgaa
     3181 gaagggtaaa tattcagttg ctagagagtt agaaacaatg agtatcgtgt atgtagctaa
     3241 gaatcatttt gacgtagaac aggctatttt tgatccagat tctgaaatag ggaaatgtgt
     3301 tgaattatca gagaaaactg ttagtacaaa aggggcgatg atcattgaga gagaaatgtt
     3361 agacgaaatc agagtaatca ttcctaaaga tacgcagatg tgtgaggaca gcataagtga
     3421 cgattaacca aaatcagaaa gaaaataagg agatattcag atgagcgaaa aatccattga
     3481 attaaaggaa tataagcgaa gcgatggaga aaaatattat cctgtagcca atttttctaa
     3541 atcagtatat ctggttggac gtgcattggt agttagtgca attggcatat atattttgca
     3601 ttgggtaaca aaggttgtac aactgcttgt agatacctta gtaagtattc tcgtctcgtt
     3661 aaatgatgtt aacggaacag atgatttagg attaaaagag atgtttaaag agttactcaa
     3721 tgcgtttttt ccaaattatt taagttattc atataatgta caaagctttt ttgcaaagct
     3781 acacttggta gatatctcta ttttagtatt agaagcagga acggtattct ttgtcggata
     3841 tttggttact ttgatgttca ggcatcacta tggagaacaa agaccattct ttgatgatat
     3901 ggaaagtaga aggttgaaaa gagaagctct agaagctatg gatgtgaagt atgatccaaa
     3961 caagattata ggagatgaaa cgaacaacaa aaaaagaaaa cgaaacaaaa cgcaattaga
     4021 atccgatgtg ctatcaacgc ttaaaaatat caaaatttct gttcatacaa gacgtgatct
     4081 aagtgtcaat gaagatattc gagaatatat cgtaaaaata ccgcaaccaa aagggaagaa
     4141 agttagagaa acagttttac gacaaattca agatttaggg tcgatcctga caagagcaac
     4201 aagagggcga gctaaatttg gggaaaaaga agacgaagtt tcaggtgatt acttcatctt
     4261 cagagcatca gtaaacattg aaagagaacg taaggaaaga gtaagtacca aaaacaagga
     4321 aacggaaaaa tcaacagcat ccattgcagt ttcaaattca caatttgctt ttccaatcaa
     4381 tctctttgca gataactcca aaaatatcca aaaccaaacc gaaaaagcta gaaaatatgc
     4441 cgaaactcaa aagcaatctt tagaacttta tctatcttct gctgacgtac ctgcaagtct
     4501 aacagatatg cacgttggga atacatctat tgaatatttg tttactttat ctcgtaccag
     4561 aaaaattcaa ctaagctcaa tgaaagaaga aattgaaact tacttgcata tatcaggtgt
     4621 aatcgtgcgg atcaacgcag ggcgtattca aattacttta ccattaggag aaaatgatcg
     4681 tattccaatt gatgtaccat caatgatcaa tagcgtgttt tagaagacat cacaaataga
     4741 aacagaaaat acaaactacc agttcacaaa cagaaaagta ccgagaaaag agaggaatag
     4801 aaatggctat gaaattgtta gagaaagtca aaaatagagc cgaaaaagct gaatctattt
     4861 tgctagataa agaacctaat ataaagctta atggaaaatt cagtgtcaat atgttgaaca
     4921 caaaggctaa agacccgaca gatgctattt ttggaattaa gcctgatggc acaattgctc
     4981 atttttgcat cagcgatacg ccacacatgt tagttgccgg aacaacagga tcagggaaat
     5041 cggtattact gaatgaaatt cttgttacag ctatctgtca tgcaatgcct gatgaattaa
     5101 agctagggat agtagaccca aaacgagtag agtttgggcg ttataaaaaa ttaccctata
     5161 tgcttgctga tccaattact gatatggatg aagcttatga attttttgaa taccttgtga
     5221 tcttaatgaa tgaacgctac aaattgatgg aaaaagcagg cgttcaaaat atcacacaat
     5281 ataacaaata cgctgaaaaa catggaatag agaaatttcc ctatgtgatc ttattggttg
     5341 atgaatattc gcaattagtt ggaacacaca aagaagttga agggctgatc gttcaattag
     5401 gacaaatggc aagggcggca ggaattcacg ttattcttgc aacacaatca cctcgatcta
     5461 cagtcgtgac aggtattatt aaagcaaatt tcccatctag agtctgcttg atggtagcaa
     5521 gtgatttaga aagtaggatc gtattagatg agggtggtgg agaaagccta agtccaaagg
     5581 gagatatgtt tatcaagttg aatggaggaa acaaaatcag agcgcaagga gcctatatca
     5641 gcaacgagga aattgaagcg atctttgatc accttagaaa tactatgcca gaaccagaat
     5701 atgttgatta ccgagcaatc gtagctgaat ctcgtggtga gggcggtgaa gacgaggatt
     5761 ttggtggaat tggtaaagct cctgtaagaa aatctgctaa aagaaatttg aacacacctt
     5821 tagcaaattc atcatctaaa gagccatcaa ttgaaaagaa gagtacgttt gctctaaaac
     5881 ttgaaaaaaa caaagaagaa aaagaaaaac aaggagctaa aacactagaa aaacttcaag
     5941 ccgaaaaatt agaaaaagtt ttaaaagcat tagataaagt tgatcctgaa ccgcaaacct
     6001 caaacaacac gaataatgaa cagccgattt catctaaaac taaaccgaaa actactgatc
     6061 cgaaagtttc agtaaaagtg accatcacaa ccgataattc tgagaagaaa caagtaaacg
     6121 gaactacttc acctatcatg caaaaaatgt taaaagccaa aaaacaggtg cataaatgat
     6181 cattgtcagt tcttttttga ggtggcttac gccatataaa tatacagatg aagtagttgt
     6241 gaaacgagct aagaaaaaag ataaaaaagc ggtgttaact cctaaacaga tgcttgatca
     6301 tggaattttg ggtgtgaagg ttttagaagt gaataaaccg tttgaatcaa atagtgcaag
     6361 agaaaagttg cttggcgctg aagcagatgt ggcatatttt tatcatgaca aaagcaagca
     6421 tcaaatagct attaaattca atccaaaatc ggcatggcgg tatcaagcaa gagatgactt
     6481 gaaacgtagg aaacttagac caatgctcta tccagaagga aaagaagcat ttgatagaac
     6541 ccacatcgtt cccatcggtt ttcatggtac agaatcagat aatcgcctat tagtaggttg
     6601 gaacagcaat caaaatagag ttcagatcaa taaatttgag aagaaagtaa gaaaattcaa
     6661 cgaacgaaaa acaatcctat ggtttgcaga tatttgtaaa cagccggacg gaacagcaaa
     6721 gtgggaagcg gtgatatttg acgaaaatgg taaaaaagta atgtctgaaa catggcatga
     6781 taaagctaaa tttgtttggc agtagaggtg aaaattgtgt taactattct aaaactatat
     6841 agagagggat ttaacgaaaa agtaagtatt ggtgaatcca ttgaatatag aggtgataag
     6901 tatattatta ttcgcattct taaaattaac aaacattatt ttagcaaatc tattagttta
     6961 acagttagag ttctagttca gaaagtcgga gacttgagtg attattataa ctgcaaaaga
     7021 gaagttattg tggataatcg atacgaccaa tcaaaagatg ataagcgaat aataaaaata
     7081 ggagatattg tctttcctga gaaagggata gcatatgaag tagtgtctat caatagtttt
     7141 cgctatgaat ttgttgattt ggttgtcgaa tattctgttc gattgatcgt tccgtggagt
     7201 aaagaagaaa ttaatgaagc attaaaagat gaacgaaaaa gtacattcaa agttttagaa
     7261 ggtgaaaaaa agtgaaaaca gaccttacta gacaagctga gaagtgttta tggcatgaaa
     7321 caaatcgcat gggtgtattt ggttgttttg aagtaactat cggttgggat ggcagagaaa
     7381 gagtagatta tataacttat tcagtagata acacagttcg atgttatgag atcaaagtta
     7441 ctttatccga tttgaaaagc aaggcgaaac agacatttat cggcgattat aactatttag
     7501 tcgttactag tgatttgtgg gagaaaatgc tacaagacga cacactcatg cgtaaatatt
     7561 ataaacaagg cgtatacgtg tttaatgaaa aatatcctag catgataagt gttaagaaag
     7621 caaggaaaca aaaagtttcg ctaggtacaa gatcaactgt attagaaagc atggttagat
     7681 cgatgaatcg tgaagttaag aaattatata aagtaaaacc attttgggag atagtagaga
     7741 taccggaatg atgagtaagg aatattatat ctattcatat atagtattaa ctgattatga
     7801 ggaggataca gcaaatgaaa aattatgatc cgaatatgcg atggggtaca catactctta
     7861 aagtgtcttt tcaacggagg ataagctaaa tgaataaaag aagaatactg tggcttattg
     7921 caggaatgta tctttgcata gcagtatctg ggtttactat ttgtcggata ataaatcagt
     7981 taaaaaacgc gattgtttat ctaggagaaa ttggttatct agaagattta aaggcaggac
     8041 tacaaataat gagttggatt ttattcgttc tgacagttgc gttagttata ttaacttgtg
     8101 aaaatttttc aacaataaaa aaacaaataa tatctaacca taatcaagag actgttaaat
     8161 caggtctaga agtagaccca gaagaggaag taactgaaca agcgatcaaa gagcaggaag
     8221 aaatagacaa aaaagaaata atgagtattt atttatctag tggagagtca ttttttcatg
     8281 aaatagattc gctgaaactt agtagtcaag aaataattga tagatggcaa aatatgtctt
     8341 tagatgggga gttaatcgtt gaggatagtg aaggttggtc agcttataag aaggacgaga
     8401 ttgtagctat cacgattttg tttgtatagt aatatatacc actagataat ctgaatatgt
     8461 tgatatgcta gtgacgtacc aaatgtgttc aatagagccg tggtcacagg cagaaattga
     8521 taaaatagta aaatttaacc gattatcaaa atttaacgtt ttaggaaata ccttttaaaa
     8581 atcacaatac acaaacagaa aaataccaaa aaattaacaa tgaacgtagg taagaacatg
     8641 acaataaatg ataataacct caaaaaatat aatgctcttg tccaacgcta tatcgaaaac
     8701 aaaataacga tgcaagaggt cgctgatgaa ttaggcaaat caaaaagcct agtatcacac
     8761 gactttaagc acaatgtttt tccgaacccg attgagctga ggaaaaaatt tcaaaagaga
     8821 gtttatcaag aaatagggaa gtggattgat gaagggaatg caattgaccc tttaattgaa
     8881 aaatatcctt ttttaaaaag caaaaataaa attcaaaggt taattcgtca aggcgatatt
     8941 caagctaaag aacctgtttt gacaaccaat gatttggcta aaataaatct tcataaacaa
     9001 attattgaag actttgaaaa aaatctttca acaactgaaa ttttaaaaaa atacaatatt
     9061 ccgagaggaa agctaacgtc gactcttttt agagaatatg gagaatcggc agttactaaa
     9121 gcaaaggaag tagcgaatga gagaaaaaaa caaaaatagt agtcgatact tagtagcgac
     9181 tgtagttttt gtttgtgtat tgattttcct gtttgatttt ttacagattg gaaaaagtat
     9241 aattggaaat acattgagtg caaaggaaat ggttgcttta ggtggtttgt atgaaggaca
     9301 tttgttcccg aatatattca tttctatcgt ttctcatatt aacttgtacc atttaatttc
     9361 taatttattg ttcttagtag ttcttgaaga agcactgaat gatcagtatg agtggttaac
     9421 attattgatc gttttaatca tatcaggatt tctaggaagc ttagttcctt ttctgctaca
     9481 aagcaacatc gtaagtatgg ggcttagtgg aggtattttt ggatggattg ctttatttgc
     9541 gattgcatac atccattttg atagcaagga acatttatta ctaagtgttt ttgtattatt
     9601 gataatcggc attttattgg tatctacttt tgaatcaggc gaggcaaatg tcttgtcaca
     9661 tgtaatcgga tttgtctgtg gaatgatctt gggatttatt ctaaagaacg tttcattgat
     9721 caaacaaaag aattaaaagg gagtgctaaa aatgccaaaa agaaaagaaa agaaaattcc
     9781 agtgtttttg tctgtctcag aaatgttatc acttcaaata ctgttaagaa aaaagattga
     9841 aacaatagac gaagaagtta gaaataaaag ggctatgcac gaacaacgtg aagcgctaag
     9901 agaacgaatc cactattttg atgatataca cactaaaatc tatgaagcac gtacaaatca
     9961 cgaagacgaa agtaaattag gattagctca acaacggata cttgaatggc ttaatacgaa
    10021 tactgatcaa gaaccgttaa gagtgattag taatcttttt gaatttggaa cgaagcattg
    10081 gtttaatgct gtagagaagg atgctgactt acaagaagcg tttgatacgc taagtaagga
    10141 tagcaaatcc atgcgagatt tattgattac ttatctaaat gagcatgagt ttgaacaaac
    10201 gaaaaccaaa aagaaaaata aagaatagaa agttgtgatg aatcatgcta ttaaccctgt
    10261 taggttattt atgtttaatt cttgcttttt ttggggcaat cgctataatt attggtcaat
    10321 ttcaaacaaa taatccgatt atgcgtaaca taggcattgg aattgtgatt gtaaccttta
    10381 ttgtgtttaa tgccactaag atttttggaa gtaacgataa acaatttggt atatcgggag
    10441 ttaataatag ttatgctcca gattctttgg gaagggtagt aatagatgga acatttgaaa
    10501 atgtaacgag ttataaaaat gtttcgctga cagtgaagga agatggcaag aaagcatcag
    10561 acgacgttct cgctactatt aagccgtctc aatcacaatt tgaagtgttt tatacagtta
    10621 aagagaatag ccctgtacac aagctgacat tggtttttaa cgttgatgga gaagaatact
    10681 ataaagatgt aaaaataaat aaaacatcaa cgaaaaagat tatcgaatca gcgaaaaaag
    10741 aagtagctac agaagaaagt acaactgaat caagttctac tgtcgaagcg gaactaccag
    10801 ataaaattga ttttgatcct gaaaaagtca aatcgtttga atctgtttct ggttctatgg
    10861 ctgatcggtt taacactgaa gtcttcaaag ctatcaaaac tgttaaattg gatactgaaa
    10921 agcaaatgat taatgttacc ttattcaatt cagttgaatc tatggatcag gataaaaaag
    10981 ttacggttgc aagagaacta ggaaaaagag taattggttt gtatcgtggt actgctaaac
    11041 cagattcaga cggtgcagat ttgccagttc atgtagttta tgaagacggt aaagtttttg
    11101 gagattctac agcaaacgaa gcattaacga ttaaattggc taaatagcca gaaagttata
    11161 ataaacaaat ggtgtcacac caataatata taggtgtgac accttggaga tgatagctga
    11221 tggtagtaaa taaagaaaaa gttagaaagt atgtgacttt aaaaatagct tctgttaaat
    11281 atttagaaaa gcgagctaaa gaagaagaaa gagcaagagg taagaaagta acagtttcag
    11341 agttgttaga agaattgatc gagaatgatc aaacaataag acgtgcattt actaataggt
    11401 ctatcagata acaatataat taataaaaaa agaaagagtg atttggtctt agactaaatc
    11461 actcttttta tataaagttt ctttacactc attccgaata atgttaaaat tctttatacg
    11521 atatatagtt ccttgtcaga ggaagagata accagcaata ttggagggat aataagtgga
    11581 aaacaataat gatattagac attttagcat tggggaaaga gttacaacag taagtgaatt
    11641 atcaaaggat tatagagata aagagggaac gattatttct cttaataaga atcgaagagg
    11701 cgtttggcaa tatgttgttg atttaggtga tggcgttaca gttgagtgta aagatagtca
    11761 actgatcaga aataatggca gtcattccat tgaaaaagca attcttgtat acccaaaagt
    11821 atcaagtaag atcatgaatg actggttgct tggaaaattt ggtgagtaca ataaaaaaga
    11881 aatcgcagaa gtgatcggca aatcttatcc aacagtatta gggtatctaa aaaagataac
    11941 gagtaaagaa gcaaaaattg aggatgtttt aacagtaaga gagcttcaat cattaatcga
    12001 gtatcggaat aaacaaataa caaatcctga gtaccttaga aaagaagatg gtctattgaa
    12061 acaattggaa caagtaaata aaatccggaa gatgcaaaag gtagctcctt taagccttga
    12121 tacctttaaa aaaatctatt tgaaagagat caaaaaagac taatgtggac tgtaacttta
    12181 ccgttccaac ctgtatctgc accaagacca agtgcaagaa ggaatgctgc tgatgaaact
    12241 aatgacgtag cagagaaaag gatttcaacc tactatgata aaaagtatct tgattatttg
    12301 aaaaaaataa agagctttat cgaagatcaa gatttattag atgatgaatt ttactcgatt
    12361 gtaaatgatc ctatgggcgc aattatggaa gtggactttt actaccagat accaaaaagc
    12421 tataaaaaag tctataacat tatgaagaca acagcgccag atttagataa tttagtaaaa
    12481 agtgctatgg atggtatttt taacatctca gctatacgag atagccatat cacaggctta
    12541 attgctttta aatataatgt ggcgaacgaa ggaaaaacag ttgtgaggat caaacgatat
    12601 tcagaatttg agtgggatac ttctgaagga ttgaacggag atatatggga agctacattt
    12661 gattttaaac cagtagcaac gccaagacca aagtctaaat ttagaggaaa tgggattatc
    12721 acttactatc caaaagagta caacgactat ttggaaaata tcaagaatac gcttaaagag
    12781 aaagatttgg taaatgatca actgaaaaag gtaatgaatg ctcctatggg ggttatagct
    12841 cagatagatt atttttacca agtgagaaaa gataagaaaa agcttgattc actgatgcgt
    12901 acttcacagc cggatattga taatttagtc aaaggaacat tggacggaat ttttaacaag
    12961 gaaataggca tcaaagatag cagggttgta gggttaattg cttttaaata caatactttt
    13021 gaaaagtcga aaacaaacgt gagactacag gagatgggct gatgaataat aatgaaaaaa
    13081 ttcttcatgt acttgatagc tttgaaataa ttcaagaaga actaaagaaa tatcgggatg
    13141 ttttggaaca aagatatgat tttgtcaatc agcaaaagag taatcacatg gatttcattc
    13201 tcaatatgaa tgacttgaag aagaaattag ttgaacgaaa agaacaagaa aagttgatca
    13261 aagcttactt tgaactcggt gaaaaagaag ttaaaaatgc aatggaactt aatgaagagc
    13321 gcagggtttt agatcagctg ttagaacaat tattggtgat gtttcaaaaa ggacgaatag
    13381 atgaggattt aattgaagaa ggattgagaa aatatccttc aaactcaggt atcggaatag
    13441 tgctgaaagc gattgatgaa gaagaaattg aagctttcat accgccagag gattttgaat
    13501 cggcaatgga atatataaaa tattatagcc aagggatcac tgcttttaga gagtttgatc
    13561 ctgaagatgt tattcatgac ttgaacaacc taaaagaatg gtgtgaaagt tatggagtcg
    13621 atgattccgg attggactac ttgatcagta ttatggaaat cgaagaagag atgccggata
    13681 agcctgatcc aacagatatt ttagaattga tccatgaagc tagaaatcca attgcctata
    13741 tttctagagg atatactgta ttggaatact ataagcctta tatatcagcg atgaaccatt
    13801 taagacgtgt attaagggaa aaacgtgaat accgctcagt gttgaatgca acgaatagat
    13861 tagaaaaagc tgtttcagag ctagatgctt attatcgtga acattacctg caagcaggtg
    13921 gtatgccaag aaatacgaaa gcaaatatca gtaggtatat aaaaaaagct gagtaggcgg
    13981 agttagccca taaaatggca aaaggtaacc tgaaaggggc attttgaatg aaaagggatg
    14041 tttttgaatt gtacgtatta gaacgtctag aagacggcat gatggcacaa gagataattg
    14101 atgaaattga tgatatgatc agcagatttg gtcaattgga gttgaatgat ggaaacaaaa
    14161 attcggtagt gctaaaaaaa ttttggttga acaatttttc gtataaggat tttatcaaag
    14221 aaaaggcaaa tacattgtac tgactaaatt cacgaggagc agtgatgaga gcttattgct
    14281 aagagcagtt aggctctttt ttgtttgtgt aaaattattt gttttataat acacatttat
    14341 acacaaaaaa atattgacgg atagtacaca aatgtggtag gatatatttg taagcaagtt
    14401 agattaacaa gttaaaggaa ggaagagcag ttatgaaaaa agaagaacta atagatatat
    14461 ttcaaattgt tgaacgagca aataatatgg gaattatgtt ttttgatcgt atttctttaa
    14521 agatggattt aagcgttgcc caccaagaat ttaatctgcg attaaaagcc ttgttgaatt
    14581 ctgatgatgt taatttcgct catgatgttg taggtataca gaaccatata gatagagaaa
    14641 acaaacgtat gggtgatgaa ttcttaccaa gatattcaag tttatgattt ggcgaaagat
    14701 tcaagagtat gtgtcattat tgataggtac tcttgaattg gaaaggagga atgaatttaa
    14761 gacaacaacg attatgatct taaaacaatc gtttatgtaa ttggcaatct tacgaataat
    14821 tttcatgcaa taatgacaat aaaaaatgaa aaggggtaaa atcaaatgaa aaaattttta
    14881 gtaggaattc tattcacaat ggggctagta ttattatcac cttcagtttc agcagaaagc
    14941 tctatttcca tgtatcgtat gtataatccc aatagcggag agcatctata cactagaagc
    15001 gttggagaaa gagacaatct gaaacgttat ggatggcatt acgaaggtgt cgcatggcaa
    15061 gctccaacaa gtggtactcc tgtttatcgt ttgtataatc ggtataacgg tgaacatttt
    15121 tacacagttg atgccggaga aagagataag atttcacaca caggatggac gtacgaaggt
    15181 gttgcgttct attcctacaa cggagcaaat agagttcctg taacacgttt atataacaaa
    15241 tacgtgaatt ggcatcacta cacgttagat caaaatgaaa agaacgtaat tagtaaacaa
    15301 ggttggaaga ttgaaggtac aggctggtat gcaatgccaa acagtgtacc aaatcctaca
    15361 cctgctccaa gcactgaaaa agtgttgaac gttcctgttg tatggcaagg aaatacaatg
    15421 ttatgcgaag gagcttcttt attagaaggt ttacactaca aaggtgtagc cacaaatcaa
    15481 agcttaacca gttttgtaaa ctatatgcca aaagctaatg acaataatcc ttatcatggt
    15541 tactcaggag agtggcgtca taacgttaat ggaacgtatc aaggcatgat ggctgaccca
    15601 gtagttaaat gggcaaaaaa cgttggcggt aatgccgtca atatcactgg ttcaggaacg
    15661 aatggaatca agaatgaaat tcgcaatggt aatccggttg taacttggat tacttataac
    15721 tatgctacgc cagagttcaa acaaatgcct tggggtcgtg cagtatggaa tggacatgta
    15781 gttctcgtgg atggcttcag aaatggtgca taccatgttg ttgaccctgt atttggcgca
    15841 aaatggatca atgcaagcac gtatgagaga gcatttaatg tgactggaat ggcggttgct
    15901 gtacgttaat tgatagaatt atcaactaaa tgaaataaga gcttaattac tgaaatggta
    15961 aataggctct tattttttta gaggaaattt ttgtaatacg aatctataac aaaaaatatt
    16021 gaatgtagta cacaaaagtg gtatattaaa atcgtagaga ttatgcgaag aggtgatttt
    16081 gatgaaccaa agaagaagaa aaaggttaga agaaataagc gagcgacttt ccgagctaaa
    16141 ggatgagtta gaagtaattc aagaagaaga gtacaaagaa aatataccta aaaatctaca
    16201 gggatcagaa agatatgaaa aagcagaaga atccgttgat caacttgaag aagctgttga
    16261 tggtttatcc gacatattag aattgattga agatatagct agctagagat gattaatgaa
    16321 gatttcagga gtgagaataa aatgattgat atttcactga tagagaagtt acttttctca
    16381 aataagtaca ccgcaaaaga gctggctaca agatggcaaa tatccgaatc agctattaaa
    16441 gcctatcgtc aaaataaggg aagcgtagga tggcgtgatt ggaaagggat cagcattgga
    16501 aaagcagaag acataatgaa acgtgaagga cttcgatcct ataccacttc tgaaaaagac
    16561 aaataactta atgtttgaaa agataaaata tctgtcttaa agctatgtca aattcaaatg
    16621 gtgaatgttt gctaaatata atgcaaaaaa aggaagggaa atattaaatg ataagcgaaa
    16681 atttgtctta tgagaataat aaacatctag ttaaagaaaa taagaaagta gaagattata
    16741 agaacatgta tatagaaatg aaaaaatcac cactgtgtat tcaatataaa aatgataatt
    16801 ggaagcaagt agtcgaattt gtaaaacctc atagtatacg aagagtacga agggggaaga
    16861 aaaaaagtga acttgtaaat agcggaagag cggtaattag agttataacc aaagattatc
    16921 cagttgaata tggagattat attttaaggt taagttatca tgcttttgtt gtgttgaaag
    16981 aagagcattt caacgagttt ttcaatttaa aaagtaagta atcatatttt tttgtagacg
    17041 tgtataaagt taactaaatt gcgaggtgat gacatggtag caaaaaagaa tactctaaaa
    17101 gaagcttctg aaaaatatca agatatgaaa agacgaggat taataccgaa agacaaaaaa
    17161 ggtatatgga gagaatttgt taaggaagtc agtagtgatc gaagtggcgg tgactttgtt
    17221 ggacgatata cagcaaaagg ctatagcaaa cgattaagga tcactgttga atatggaacg
    17281 aatgaatcag ctacagagct gaagcaaagg tacttagata cacgaaggga agcttcaaaa
    17341 aaatataatg atttcaagag cagaggtctg attcctaaaa agaaaaaggg aatttggcgt
    17401 gagtttttca aggaagaaga tagcaatcgg aataagggag acttcattgg acgatacaca
    17461 gcaaaaggct acactaagaa tttagggatc aaggttgaat acggaactgg tgaaagctca
    17521 tgggaattga gaaatcgcta tttaaataca ttgttagaga aatataaagc agaattattg
    17581 gagttgttcg atgttaatga ttttatcgag atggctgaaa agtatggtca tgaagaaggt
    17641 tttatgatca atgacattat ggttattgat aattttacgg atttattagc acttgcgaaa
    17701 aaagcaaggg atttaagaga aacgcagatg tatctattca gaaataaaaa cggaagaaac
    17761 cggagaactg aatcgagtga gattgaagat tactacgatg cctttacaca cgatctggga
    17821 gcctttgtag acgagataag tggtgtaatg aacaaatata gggtctagaa taaaaaagcc
    17881 ggagatgagc agataaatgg gaaacaatag catatacaca cctgaaatgc agaataaaat
    17941 taaaagactt gtcggattag ggtggatgac aagaggaaaa ttagattctt ttagtacgat
    18001 caaatatctt ttgatggaca tttaccgtca gaacaagcaa acaggaatac attttttggg
    18061 ttatgataag ccggaagatg cacctttctt tgttccaagt tcagcctata gagccagtac
    18121 ctccggtagc atgtataact tggatttgtt tgaggaagga gcaaagatga tcgaatatga
    18181 tttcaacgaa gcctattcga ggatcatgag aaattatcct ttgccgtcaa acacttattt
    18241 gaaagcaaaa tataatacag atcaagctat tttgagagcg ataaatagac caaaagtaag
    18301 accgtatgaa aatttaaaaa cgtatatgtt ctttttggtt aattttgaag gaacagccaa
    18361 agaaggaaca tatgctaatc caaaatcaca gtcgatgttt gcggattatg gcgaaaatcc
    18421 caaaacagct aatgagccta tttggttatc ggagattgag ctaacactta tccttgatta
    18481 ttatgatatt aaagagatgg aagtcctaga tagccacaca tttttgacga aaaaaggatt
    18541 actcaaagaa tattttgaaa gaatcgagcc attaaaaact gatcctgata cgttgaagct
    18601 ttataaaaat cttagaacga aagtatttgg tgctatcgga caaaaatcat taagagaagg
    18661 agaaacaagc accttcgata aatacagtat gtataatcga gcgtattcct ccatggtagc
    18721 aggcgtattt agagatcgta tggttagata tgaacagaaa tacgttcata gcgcatatgg
    18781 gctattaaaa attcggactg atggattgta tttcagatcc gaagtaccag agtttgaaga
    18841 gctgtataaa cttggagtag ttaagaaaaa agttcataca atatccaaag aagaagtcga
    18901 aaaagcttta gaatttaaaa acaaaggatt tgaaaataag ggctcctttg ggaactctaa
    18961 agatgaagtt aagggtgttg tgatagacga tgatttcgag tttgattttg actaaatact
    19021 ctttggaaaa gatggtagga ggtaaatatg aagaagaaaa ttgtggaagg aaatgtaggt
    19081 gttattattc taaaagattc aactgactat gctaaatcaa tggcactaaa agacgaaaaa
    19141 gttgcacgca tggataaaga tttagttgaa tttctgatgg atgatacaga aagattaaaa
    19201 ggggatcagt ttttgagttt aatgaaagac aaaggcttaa acacatatgg tttatttgat
    19261 tataagaaat tagctataga gtgggtaaaa gaaggagaaa tattttttat tgaagaatgg
    19321 gaaggtactg agaacttagt tcttcatcag tatcttttga aagcttaatc taagtaaaac
    19381 agttctgact agttatgctt gtcagaattt caaaggatgt tttccataac catcatcatt
    19441 caaaaataaa ggatttgaaa ataagggttc ctttgggaac tctaaagatg aagttaaggg
    19501 tgttgtgata gacgatgatt tcaagtttga ttttgactag atttttttga aaggtttaag
    19561 agtaaacaag gtaagaacta agatcatgaa gattgagagt aaagaagata gaggacgaat
    19621 gtatgagaac acactatatt tatttagaag ataaaattgt agatgaaatt ccggcaaaaa
    19681 gtcgagaaca agctagaaaa ttatttattg aaaaatacaa agagatcaat ggagatagtg
    19741 aagttccaaa agtggtcgta acagttagaa aaaggcaaac aaaaaagaga agtacgtttc
    19801 atgtcagccc tgatatatat aatcttatag agcgacatag aagtgaagat aatatttcta
    19861 tctctcaata tattgctaca gttattgatg aattgatttc aggaaagtat gactatgctt
    19921 ttgattttga ggatgaaaag gattacaaaa gtgttgctgt ctttcaatct gattttgaga
    19981 agctaaaaaa ctatgctggc aggagaaatg ttaattttgc aagactacta gaagagttct
    20041 actccgaaga gttgggagta ggaaatgaat acaagagaga aacttatgcg aaattgtctc
    20101 ataagttatc ccaagcgctt aaagaagtga cagaagttaa agcggaacta gatgctaaac
    20161 ttgaagaagt gaaaaaatat caaacagaga ggaagaacaa catgaagaat caagtggaat
    20221 ttgacgaaca gaaagaattt gaaaaagcaa gaaaacgcta taacaaaatt atgaatgaac
    20281 ttagctatga acacttaaca gttcatgaag aattgagtga gggtacagaa aaatatacga
    20341 tcgcagatat ggttaatgaa gtggaaactc ttttagcttc tttctttgaa aatggtcacg
    20401 taaacgatga atggcgaaaa gaagaaccta aaagggttaa cgcttatatt gctcgattaa
    20461 aaaggtttat tgataaatat aaggagaata gtgacaaatt ttctaaagat aaataaggag
    20521 agagtgtgat tacatttgcc agaaatcgaa ggaatatttt cgccaaaaat aggtaaaaaa
    20581 cagttagatt tcagcagttt tccgagcgta aatagagatt ataatctagg tgatgaggta
    20641 cagatactaa atattgattt caaagaacta gacttcgctg ttgctgtttc caacaaaccc
    20701 aaggcattgg aacgagtgaa aaaaatatac caagagaatg atcaaaatat caaaaatatg
    20761 atcaaaaagt atagacgaga taattcaaaa gttgaagacg ttttattcaa cggtacggca
    20821 aaacatgatt tgaatgttta tatggcatta ggagtggctt tgtactgtaa ttcaattaat
    20881 cagacagata ttttggttaa tatcgtgagg aatagctatc aaaaaatatt aaaaataagt
    20941 agcgaaacaa acccttatta tgcaatgaaa tatcttgggg ataatttatt aaatgataaa
    21001 aaattgatgc acaacactga agagctgtta tctaatttgg ctgcgattaa ctttctattc
    21061 aaaacaggtt ctgaagatta ttttatagat aacgtactaa aaggtgctta cggaacagca
    21121 ttttatccgc tttacaatat gaaacagcat aaagccgaac aaaaggataa caaacttgaa
    21181 attaaaaaga tgaaagaact attaggtata ggatttgtaa agcccaattc aaaagtcact
    21241 attgcttcgt tagcaactac taacaatgat gggaaaatag taaatgggtt tgacgattca
    21301 gatatagata tattgtcaaa atcatatttt tctttgtatg gagattttgc agacttgatg
    21361 ggtattgata gttacttagt caataatttt gagatcaagg gtagcgagct gaatgatatt
    21421 gtgtatttcg ttttaatctc tctatatgca attgatcaga ataccgagta ccgacaaatt
    21481 gactatttgc tagtttttta tagtttgtta tatcagttcg ttttaactcg gttgtttaaa
    21541 aatgctaagg aagaactgat cctgaattct gattatgaaa tagcaaaaca aattgcaagt
    21601 attagaaaag aaaacaaatc tgaagtaatc aaattaagaa aagaattaag tattgcgagt
    21661 aaagaaaacg gtcggttaga atctttagtc aaatcggcaa aaaaagaaga ggaacttttg
    21721 aagcaagaga ttaaagaatt aaaaggaatc agaaaagaat ttgaacagct gaggtttgaa
    21781 aataaagtat taaagcaatt gcaggaacaa gaacatagac aatcattaaa tggtaaaact
    21841 gaagagcctt cactgtctga aatgatagct tatctaaaac ataaaaacat tcttttagtg
    21901 ggaggacatg taaattttca aaataaatta actgaactat taccaaatat taaaatgttt
    21961 gatgtaagcg aaaataatgg aaagatactt aaaaatttga atacgtgtga tgcgattttt
    22021 ctatatacag a
//
LOCUS       DAFGFZ010000047        21937 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000047 DAFGFZ010000000
VERSION     DAFGFZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.047"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(40..483)
                     /locus_tag="LR726_001475"
     CDS             complement(40..483)
                     /locus_tag="LR726_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6216575.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(499..1659)
                     /locus_tag="LR726_001476"
     CDS             complement(499..1659)
                     /locus_tag="LR726_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL6216576.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2166..3866
                     /locus_tag="LR726_001477"
     CDS             2166..3866
                     /locus_tag="LR726_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216577.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(3968..4801)
                     /locus_tag="LR726_001478"
     CDS             complement(3968..4801)
                     /locus_tag="LR726_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL6216578.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5105..7270)
                     /locus_tag="LR726_001479"
     CDS             complement(5105..7270)
                     /locus_tag="LR726_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6216579.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKNPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7530..9824
                     /locus_tag="LR726_001480"
     CDS             7530..9824
                     /locus_tag="LR726_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL6216580.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9817..10482
                     /gene="pgmB"
                     /locus_tag="LR726_001481"
     CDS             9817..10482
                     /gene="pgmB"
                     /locus_tag="LR726_001481"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL6216581.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10475..11482
                     /locus_tag="LR726_001482"
     CDS             10475..11482
                     /locus_tag="LR726_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL6216582.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11504..12523
                     /locus_tag="LR726_001483"
     CDS             11504..12523
                     /locus_tag="LR726_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6216583.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="LR726_001484"
     CDS             complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="LR726_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL6216584.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14280..15701)
                     /locus_tag="LR726_001485"
     CDS             complement(14280..15701)
                     /locus_tag="LR726_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6216585.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="LR726_001486"
     CDS             complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="LR726_001486"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL6216586.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18112..19368)
                     /locus_tag="LR726_001487"
     CDS             complement(18112..19368)
                     /locus_tag="LR726_001487"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL6216587.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(19480..19685)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19948..21249
                     /locus_tag="LR726_001488"
     CDS             19948..21249
                     /locus_tag="LR726_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL6216588.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21318..21917)
                     /locus_tag="LR726_001489"
     CDS             complement(21318..21917)
                     /locus_tag="LR726_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6216589.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         6610 a         4588 c         3922 g         6817 t
ORIGIN      
        1 aaaaattagg ctgggagcat tatttcgtta ttctgttcat caaaaatcag cgttttgatt
       61 ccatttcatc acaagttcct cctcatttac tgcttcatct ttttgctgtg tttgaattgt
      121 aaaaccttgt gaaagataga aatgaatagc tgattgattt ttttgataga ctgtcaaagt
      181 cagtgtttta ttggtttctt ttattttttt cattagttgc gtaccgattc tttgttgccg
      241 gtattccctt ttcacaaaga ttcctgcgat aaactgatca atcaccccag caaaaccaat
      301 aatttcttga tctatttctg ctaagtaaat cactgcatct ggtaaagctt tcctaacaga
      361 ttctacttga ttctcccaat aagttgatgg aatgaattca tgagaatcca agttagcatg
      421 cagccaaatc gatagaacag attccagttc tttggcagac aattttgttg ttgtgcggat
      481 cattttttct ctccttactt attcattgat tttaaaataa aaaccatttt tttgaagctc
      541 ttttacaagt tttcgcccct caaaaaagta tatcttctct tgttttactt ttctaatacc
      601 aaaagaaaca gcaactttag gatcatcagc tgaaacgatt cgataagttc caccaggcaa
      661 ttgatccagc ttgactttcc catgatggtc gctttgtact gaaaacggca caccatttct
      721 agtaagtgtt tttttcttat gtttcaaaat gaatgaacta ccatttaccg gtttccctga
      781 cgaatcacta ataataaatg aaaaatcaag cactttttta cttaatctat actgatagat
      841 tagtcgtccg ataaacacta acagtatgac tacagctatt atcgttcctg caagaataaa
      901 ccattgccgc atggttcttt cctgatttcc ttttccttct tcttttttta ccgattcgtt
      961 ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg ttgatcatat acggcgtaca
     1021 tgtaagcaag gtaactaagt cttctccttc tgaaatcgtt agaacagacg tgtcatctgg
     1081 cagtaccact tgtatatttt taacttgata agccaatttc tcccccaaaa cagtcaaaat
     1141 aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat aaccgtcgat taggtaaacc
     1201 gctgtgagca gagatcactg aatgcgtcga atttccacca agcgggaaag aggttccttg
     1261 caagacgcct gctccatttt caaggatcct accattgagc cgatcataaa gtgaaatatt
     1321 taattggatc ttaggtatat tgatacttcc gatcagccat tcctctaatt ggctggatgt
     1381 tgtactttca tttctgttct tttcattgaa aggatcagta ccaggattga atccttggtc
     1441 agttaatcgt tggtttttct cctccatttt ctttcgctgc acttcaattt cagctgcggt
     1501 ccgtttttgt acttgttcga tacgtttttg atcaataaaa ctatttaaag aatccacata
     1561 aaatggatat aaagcgatca gtaaccctac gacgatacat attaataaaa acaaacgaaa
     1621 caaaagtcga cgagactgtt tttgcttact ctgcttcaca tgtgtcctcc cttgcaagtt
     1681 tttttgttct ttcttttatt atatgggttc aggaaaaaaa tagaaaatga aagccgatga
     1741 ggttgtgaaa aagctgtcct gcatcaagta ataagtacag cccaaaaaaa tagctactca
     1801 tattttttgt tgggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
     1861 agaggttgtg aaaactgcgt tatgctttga gtaataagag cgctagtcag aaaatagctt
     1921 ctcatatttt ctaggctagt gagcttatta ccgagaagca gctgattgaa caccgtttac
     1981 cgagaggtca tgaaaaagct actttgtttc gagtattaag aaaaattctg tttcaaaaca
     2041 aggagatgca acaaaactta tgtcgcatcc tctctcctta gaactttcac ttatgttttt
     2101 cttgtaaaat tcataaatat tttcatgaat tttctaattt cttgcctacc tctcgcatag
     2161 gatatatgat gaatttatct aaagtttgca aaggagttcg acaattgaaa aaatatggcc
     2221 catttttagc aatttttttc attgctttgg catttctcat gcctcattta ttcacacata
     2281 aactaatttt gggtgctgat gccccattcc attacaatcg gttctatgat gccgcgcaac
     2341 aaattaaata tggggatttc cattattttc cttcccttta tggattccag caaagtggca
     2401 ggatcgtgaa tgccgtttac ggcccatttt tcgcttattt acaaggattg atcgtgttgc
     2461 ttgcaggtag ctggtttcat tatcagattc tttcaaattt cattctttat cttatttcag
     2521 gattttctat gctgatttta ctgagatatg tcaatgttcg aaattgggta agtgtaccag
     2581 tagccgtttt attcatgagt acgtattcta ttcaatactg gtctatcaac caaggattca
     2641 ctagctgggg gacttgtttt tatcctttat gtatgatccc tattgctgat ttcgtccttc
     2701 ataaaaagtt ccctgttatc aaagtcgctg tctcagtggg acttatgaca cagatccatt
     2761 tactgtcttc gatcatgtta gctttgatgt atatcccttt ttatctttat tactttttta
     2821 accagactga aaaaggaaaa gcactgcttc aactgattaa gtcggtcttg ctttattttg
     2881 ttctgacagc aaatatttgg gcaggattag ctattgttta cagtgggaat aagattcttc
     2941 ctccctttgt taacagagaa atgagtcaaa aggcgataga tctggatggc agttattggc
     3001 tccattaccc taaggccttt cctatattga tattatcagg ttttttgctt tttattttct
     3061 ttcaacaaaa gaacacctta ttccaaaaaa tcgtgttgta cacaagtctt gttttctttt
     3121 ttctttctac tagcttgatt ccatggacga cattgatcga aaaaaatgtg ccttttatta
     3181 gtttgatcca gtttccattt cgatttttcg tgccatttac gctattgctt ctactttacc
     3241 ttgccctttc actgaaccag tggtctgaac aaaagtggtt ccgtctttta agtatatttg
     3301 gcattcttat tttttcagca cagacgatcc agaacttata ccagcatctg gaaaagtggg
     3361 aaaatgaaac atttgtcagt cgacatactt atctattcga tacgccggaa gaagcaagga
     3421 aaacattctt cagcaaagat atgtctacca gcttgttcgt tttccaaaaa accacacctg
     3481 actatctgcc aatctataag gagacaaaag acaataaata cgaccggtat aacgaagaaa
     3541 tactagaaaa cgaaacaact tttataaaaa cacatccagg cggtcttttg acaatcaagt
     3601 ggaaaaacac tggggacaaa actgttcata tccctgttat cgtctacgat cggactgtgc
     3661 tacagcaaaa tggaaaaaca ttgactgatt atgaagtaac agatatcggg accccaatcg
     3721 tgaagcagca aaaaggaatc aacgagctca cgcttcatta tcaaacgcca atctatttct
     3781 atttcattct ttctcttact ttgattggct ggtttacctt gctatgttta tttatttatc
     3841 atagatacaa gcttttacgt gcttaggcaa aagggataaa tacatccgat caaaaaactc
     3901 tccaaacggt ataatcgttt ggagagtttt attttaatca gtttttttat gttatatgtt
     3961 atatttttta ttcgttcaca tctaattcga tttccacccc gtagtgatca ctgatgattg
     4021 gactatcgat cccatcaaaa atccgttgat attttttaac ctgcattcct tcagggacga
     4081 aaataaaatc tatacgcaat ttttctgtat tttgttccca accatctatt ttcttttcca
     4141 cagtagctgc ttttcctttt tcattcgcga caacatacga atcttgaaga agaaggcaac
     4201 tttctcttac agtataataa ccttgagagt ctactggatt attgaaatca cctaggaaaa
     4261 aaagtggtac gcgagtttcc aaaaagtagt tttccaagtt tttccactca aaataaaagc
     4321 cacttttttt atctgtccac caagaaaagt ggcagctcga gacaaaaatg tcctgatcat
     4381 ctatctttgt tttcccaatc aagatcttgc gtgtctgata atcttctttc ttttgaaatt
     4441 gggagacggt aaggacctga ctttccaatg tttttttcgt aagcagtgca tttccttctt
     4501 catatttatc ataaccgatg tggctcattt cccaactcca aaaatactct tttccgtgtt
     4561 cctttaaaag ctgaactaaa cggtaggcaa aattatcttt acggataggt acttgattct
     4621 ttaccggaca gaacttcatt agcttcgcag gttctacttc ttcagattcc agtaattggt
     4681 tgatttcttg taaagcaatc acttcataat cttctaacag gatcttatct gctaattctt
     4741 gcaatttttt ctctggttct ggctcaagcc agctatgcgt gttgagtgtc atgaatttca
     4801 ttggttttct ccttgtcttc atttcgaaac aaaagggttg tgacaaaatt tttgtcacaa
     4861 cctctccagc aaatagtgtt ctcctgctaa ctcctcagta ataagctcaa tggaccagga
     4921 gctatttttt gtttggctgt ttttatcact tggtgcaggg cagctttttc acgacctctc
     4981 cactaaacag tattcaaact gctgcttctc ggtaataagc ccaaccaaac gaaaaatagg
     5041 aggagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct
     5101 ctccttacat tttaatggag ccgatcactt taccagctgt cactgtacct aattgcttga
     5161 tcactacttc ttctacttta tcgtttgtaa atacgatcat gatcgttgga tctttttctt
     5221 cgttttggat cacatcaaga tccatctcag ctaatttcgt tcctgttcct acttcttggc
     5281 cttctttgac agcaatctca aaagctggct gattcatttg gactgtatcg attcccatat
     5341 ggatcaaaac ttcgatacca tctttcgttt ccagcccaat tgcatgtttg ctagggaaaa
     5401 tacttgtcac ttttccagcg atcggcgcaa caacttctct tgatgttgga ataacagcaa
     5461 agccgtcacc catcattttt tgtgagaata ccggattttt tacttcagat aaaggaatca
     5521 cttctccaga agctgcggaa actagttctt gttctatacc taagtattgc ttactacctg
     5581 cttgtccatt tcctgtagaa accatttctg gctctggaat ctctgctcct gattgcagca
     5641 gatcttcgat atctgatttc aagacatctg cttttggtcc ataaactgct tggactccat
     5701 tgtctttgac gatcaatccc atcgcccctg ctcgtttcca ggcttcttca gtccctactt
     5761 tttctcgatc atttacactt acgcgtaacc gagtcataca agcatcgaca tctttgatat
     5821 tttcttttcc tcccagcaaa tgaatgatct ggaccacttg ctgatctgct gttcctgttc
     5881 cgctgttaga tacaacggtt tcttcactgt cattgtcgta atttccgtta cgtcccggtg
     5941 tagcaaagtt gaattttttg atcaagaaat ttgctaagaa atacgtaaca acaccaaaag
     6001 cgatcacaca caacacgaag ttgataagat ctccacctag acccgctttg atagctaacg
     6061 gtgtccgagt cagcaattcg atattcccaa atgaatggac ccgcaatggc aagatatctg
     6121 ccatcgcaaa tgcagcccct tggatcactg aataaatgac atataatggc acagcagcaa
     6181 acataaacat gaactccaat ggttctgtta cacctgttag aaagacagcc aacgctgctg
     6241 aaaaatacat tgatttatat cttgctttct tatcaggatc gacattacga tacatcgcaa
     6301 aagccattcc catcaagata cctgaagaac caatcatttg tcctactttg aaacgagccg
     6361 gcgtccagtt atgcaacaca aactcatatt gcgacatatc ccctgcacct ttaagattca
     6421 ctaaatcagt ggcccaagcc aaccataacg gatcttgccc aaatacttgt gtccccgcct
     6481 gtgcgcctga taaaatctca tacgatccgc caagctgtgt atagttgata ggaatcgtca
     6541 gcatatgatg caatccaaat ggcaatagta aacgttctaa tgttccaaat aaaaacggtg
     6601 ctagaatcgg tgcgctttct tgtgattctg caatccataa accaaaatta ttgattccag
     6661 cctgtacgtt tggccacacc acagcaagaa tcaatgccac gattgttgac cataagataa
     6721 caacaaacgg aacaaagcgc tttccattga aaaatgacaa agcatccggc aattttctat
     6781 aattatagta cttattataa gccattgccc cgacaaatcc agcgatgatc cctacgaata
     6841 ctcccatatt caaagctggt gcttccaata cacttgtaaa aaatccttta accatgatct
     6901 tcgtaccgaa cagagtgtgg gtaaatgcat cttcattcgc aagcatgtca cttgtgacac
     6961 caaagatagc cccagtgatc cgattgatta aaataaatga gatcccagct gcaaatgctc
     7021 cgcctgctct ttcttttgcc caacttcctc ctattgctaa agcaaataaa atatgaagat
     7081 taccaataat tgcccaacca atattttcaa ttactccgcc cgttgtgaca agcggcgtcc
     7141 aatcaggatt gatcaaggga attgatttcc ctatactgat catcaaccca gctgccggca
     7201 taactgctac cacaaccatt aaagcctttc caaatttttg ccaaaactca aaactaaata
     7261 tctttttcat tccgtttagc tcctttacgc aatcggtttc gttaaacgta ttatagcgtg
     7321 cgttttcttt tttgtaaatg ctttattttt tatttttctg tctataccat tctataaaac
     7381 ctataatacg tgtgttccct tctataattt tctcctaaaa atttattttt ttaagctttt
     7441 ttctatgaaa tattttcttt tgtctattga cttgtgcaat cggttgcgtt tattatgaac
     7501 gtataaaaca gctcattagg aggagaaaaa tgaaacagat caaacgacta tttgctattg
     7561 atccatggaa aatcagcacg acacaattag ataaaaaaaa tcttcgactg caagaatctt
     7621 taacaagtat tggaaacggt tatatgggga tgcgcgggaa ctttgaagaa acttactcag
     7681 gggaccacca tcaaggtact tatttggcgg gtgtctggta tcccgataag acacgtgtcg
     7741 gctggtggaa aaatggttat ccagaatact ttggaaaagt gatcaacgcc ttgaacttta
     7801 tcgcgataga tatttcgatc aacgatcagc tgatcgactt ggcttctttg gaaccagaag
     7861 acttttactg ggaattagat atgaaaaatg gtgtattatc tcgtacattc acaatcacta
     7921 cttctactaa taaagtacgc atttccttcg aacgttttct gagcattgtc aaaaaagaat
     7981 cagcttatat ctatttaact atagaaatgc ttgaaggtag cggaacagtc aaactcgtat
     8041 caaaattgga tggaaatgtt caaaacgaag acagcaacta tgatgaacat ttttggaatg
     8101 agattgatcg tggaactata gaagaaatcg gctatttgac gactaaaact attccaaaca
     8161 actttgggat tgaacaattt actgttactg cagcaatgcg ccatttcgtt aatggaaaat
     8221 cagtcatccc tgactatcac gaagatactt tagcattaca tggttgcttg tctgcagaat
     8281 tatctcaagg tgagacatgg tcagtggaaa aagaaattat cgttcttact agtagagata
     8341 ttccagaagc gcagcaagca gaaaaagctg tctcagaatt aacgaaccag acaaacaatt
     8401 atatccaagc aaaagaagag caaacagcgg cttgggcaga acgatggaag cttgccgatg
     8461 tggtcatcga aggagacgaa gaagcacaac aaggtattcg attcaatttg ttccaactat
     8521 tctctactta ctacggcgaa gatgaacgat tgaatatcgg ccccaaagga ttcacaggag
     8581 aaaaatacgg cggagccact tactgggata cagaagcgta tgctgtccct ctttatcttg
     8641 ctttagccaa acctgaagta actaagaatc tgctgaaata tcggcataat caactgccgc
     8701 aagcaatcca caacgctcag caacaaggat taaaaggcgc gctttatcct atggtcacct
     8761 tcactggtgt cgaatgccat aacgagtggg aaatcacatt cgaggagatt caccgaaatg
     8821 gcgcgattgc ctatgcaatc tacaattata cgaattacac cggcgatacg gaatatttga
     8881 aaaatgaagg actggaagtt ttagcagaaa tcgcacgctt ctgggcagac cgtgtccatt
     8941 tttcaaaacg agcaaataaa tacatgatcc acggtgtgac cggcccaaac gaatatgaga
     9001 acaatatcaa taacaattgg tatacaaata cgatcgcagc ttgggtcttg cgttacacgc
     9061 gagaaaatta tttaacttat caaaaagaag ctgctatcac tatcagcgaa gaagaactag
     9121 ccaaatggaa agatatcgca gagaacatgt acttcccagt tgacgaagaa ttaggaatct
     9181 ttgtccagca tgacacattc ttagataaag atctcatgcc tgtatccgat attcccacaa
     9241 gcgatcttcc tctgaatcag cattggtctt gggacaaaat ccttagatct tgcttcatca
     9301 agcaggcaga tgtactacag gggatttatt tcttcaatga tacttttaca aaagaagaaa
     9361 agcaaaaaaa ctttgatttc tatgaaccaa tgactgtcca tgagtcttcc ctttcgccaa
     9421 gtatccattc gattttagca gcagaattag gaatggaaag caaagctgta gaaatgtacc
     9481 agcgaacagc tcgcttggat ttggataatt acaataacga cacagaagac ggcttgcaca
     9541 tcacttccat gactggtagc tggttgtcga ttgtccaagg atttgctcaa atgaaaactg
     9601 cccatgatca gttgagattc gcaccatttc tgccaaaaac ttggaaagct tattctttcc
     9661 atatcaatta tcgcggacgt ttattgttca tctcagtcac agatgaacaa gttactttcg
     9721 agttattaga aggttctcct ctagaaattt acgtctatga acagtcttat tctttagcgg
     9781 atcgtctaga aataacgcta agaaaggagg aagcaaatgt ttaaaggggt attgtttgat
     9841 ttagacggtg tcattaccga tactgctgaa ttccattatc gtgcttggcg aaagcttggt
     9901 caggaaatcg gcattacgat cgaccacaca ttcaacgaac agctaaaagg cgtcagccga
     9961 gaagactcac tctctttgct tttagcttat ggcggaaaag aacactcttt ttcaaaggaa
    10021 gaatttgcag aattagcgaa aagaaaaaac gattattatt tagagatgat ccagacgatc
    10081 gaaccaaaag atgtcttccc cggggtcgtg cctttactgg attctttaaa agaagaaaaa
    10141 atcaagattg ctcttgcttc tgcaagtaaa aacgggccat tcctacttga aaaaatgggt
    10201 cttacacctt actttgatgc tattgccgac ccggcagaag caacaaacgg aaaacctgcg
    10261 ccagatatct tcctccttgc agcaaaagct gtcggactaa aagcagaaga atgtatcggg
    10321 atcgaagacg cgcaagccgg tatccaagcc atcctctcaa gtggcgctca accaattggc
    10381 gtgggacgtg ctgaagacct tggagaggag atcccgcttg tccctgatac gacttttctt
    10441 acgatcgatt acttgaaaga aaagtggcat gaccatggat aatccagaga tctccgtcgt
    10501 accagttcca gagactgaaa taaagaaaat cacattgaag aacgactact tagaagtcgt
    10561 tcttctcaat tacggtgccc gcctgcacca aatcttcgca ccagacaaag aaggaaaatc
    10621 agaaaatgtt ttattgtctt acgatcgttt cgaagatgtt ctaacagaca aaagcttttt
    10681 tggagcaact gtcggacctg tagccggaag gatccgggat gcttcttggg gtgagcatag
    10741 attggagaaa aactgcggat cacaccatat ccacggtggc acgaaaggct ggtctttcca
    10801 attttgggaa gtagaagttt tcaaaactcc atcatcaata ggtgtggtct tttacttaaa
    10861 agatgaattt tctacctatc caggaccaat cactgctacg atcacttacc gtttaacaaa
    10921 aaacgaatta gagatgacaa cctctgctag tagccttgtt gaaaccatct gcaatccgac
    10981 caaccatgcc tacttcaacc tttcaggaaa tggaacaaga gacatctatg aacatcagtt
    11041 aacggtcttt ttagatggtg tgcttgaatt agatcaagaa aaactgccta ctggaaattg
    11101 gacaaaaaaa gaaaaactgc caatcgactt tagaaaatcc ccaacactgc aagagatcct
    11161 tgcatgttat ccagatggac tagatgatgt atttcttttg catcatccta ggctttccaa
    11221 aacttcctta caattattcg agaagcattc tggtcgccaa atgactatag caacttctaa
    11281 taaaagtatg gtattatttt ctacgactgg attcgaagct gatttctctg tcaatggaaa
    11341 acagatgcat tcaaactacg gattagcaat cgaaccgcaa gaaatccctg atatcgttca
    11401 tttccccaaa tttggatcga tcaacctcca tcctggtcaa gaacggatca gtcagacgat
    11461 ttatcggttc tctgcacaat aacaagagaa aggtgttttt atcatggcta ttaccgtaaa
    11521 agatgtggcg aaaaaagccg gtgttgccac ctcgaccgtc tctcgtgtca tcaacgatca
    11581 tcctagtatt tccgagacaa caaagaagaa agtccgaaaa attatggatg aactaggtta
    11641 tgttcctaac atgactgccc gcaatctagg aaaacgcatt tccagtgcca tcggcgtaat
    11701 cttaccccct cttgattcaa aagagcggat cggtaaccca ttttatcttg agatcatgga
    11761 agcaatcaac gaagaagcgc ggaattatga tatgactaca gcaatcgcta ctgctaaaaa
    11821 ttttgaattg ctgttagaga acgtccagcg tatgcattta caaaaacaag tagatggttt
    11881 tattctagtg tattcagata gtcaagatac tgtcattgat tatttatacc aaaaccacat
    11941 ccctttcact ttgatcggac agccttatca tcatgagaac gaaatcgttt atgtagataa
    12001 tgataaccag cttctaggta aacaagcaac agaatttttg atccagaatg gacataagaa
    12061 tatcctgttt gtgacaaaca cgactcatga aagtttgtat ttcgaacgat atttcggtta
    12121 tcaaaaagca atgatgatgg ctggtcttcc tgcgttatcc agtgtagtac tagagcatcc
    12181 ggaggattat ctgtcctttg agaaaacgtt gaaagaaaac gaagccacag cattggtggt
    12241 cattgacgat atcttcgccc ttcgcacgat gcagttggcg caaatgtatg gctatgtcat
    12301 tccagagaca ctctccgtca ttagcttcaa caactcgatc ttctcaacat taacacaccc
    12361 ttaccttaca agtatcgata tcgatatctc cgaattagga cgtgtcgcta tgcaaaagct
    12421 gcgcgaattg atccatgaag aattgagcag tggcgtacgt ttggtcatcc ctcatcgctt
    12481 gatcaaaaga gagacagtcg ttcctttaga aaacccatac taaatagctg actcactaaa
    12541 tacgctgacc atttatccta tggtcagctt tttatgtaaa agaagatact acttctaaca
    12601 cattgtttat ctgtaaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    12661 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac
    12721 aaaaatttaa aaagcaattt tcttgagttt cttcttattt cttggagtta aacgcttctg
    12781 tcccagcctc ttttttcctt ccttcaattt tttacacgtc gacgattcaa taaaaaaccg
    12841 cctaatattg taaagacagg tgcaagaatc gagaaaaagg cgaatggcaa gtaatgcaac
    12901 gcgctaactt tcatcgttcc cataatgaat gtgccgctga ctccccacgg tacgagagaa
    12961 ttgacggtcg cacctgaatc cgctagtgtt cgtgtcaaat aggtttttga taagccaacg
    13021 tcatcaaaag aacgcttgaa tgtttctcct ggaagaatga tggacagata ctgttctcca
    13081 acgatcagat tgattccgat acagctcaag gcagtaaacc cgactaaacg agaaggtcgg
    13141 tccatctttt cttttaattc ttgaacgatg gtatcaacga tcagatattt gatcagtaac
    13201 ccacctaatg ccaatgcaag taaaatcaaa ctagcagaac ctaacatact catgatcccg
    13261 cctcgagaaa gcagttcatc tagctttgga tcaggtgtat ttgcgacata accattcatc
    13321 aagatgtctg ctgcttttcc gaccgtcaaa cttttctcat ggataaaaga aagaacgaga
    13381 gcagctgtcg atcccgctag taatgtaggg atagccggaa tttttttgat tgccccccca
    13441 aataaaataa ctagtggaat caatgtccaa ggagagatcc aaaagctcgc tttcatgttt
    13501 tggatcattt cattgatact agctagatcg gctgaatgac tgttatgtcc taatatcgta
    13561 taaaaaatca acgaaaggac gaatgctggg aaatccgtat aacgcatacc agcgatatgt
    13621 tcgaatagat taactcctcc aatacccgct gccaagttgg tcgtatctga taaaggcgag
    13681 cagttattcc ctaaaaaggc accggaaacg actgcgcctg tagtcattgc atcattgaat
    13741 ccaagtaaat gaccgatccc caaaaaagca attccaatcg tcgaaatcgt tgtaaatgaa
    13801 ctgccgacag tcactccgac aattgcacaa acaagaaatg ctgtcggtaa aaagaaacgc
    13861 acagaaacaa gtttaaatcc ataaaccata atcgtaggga tggttccaga taaaatccat
    13921 gaagcaacta agacaccaat caaaagaaaa atcaggattg gaatgatccc tggagtgatt
    13981 cctttgatga tcccgtcatg gatctcatcc catgtgaact ttttaaatct gccataaaac
    14041 aacaaacaca tgaaagctgc aaggatcggg atatgtggcg agagctggaa tccgatgatc
    14101 agtactccta ggagtgccaa caacacgatc aatacagcga tactttcttt taaagaaata
    14161 tttttattca tcgtcgatct cctatttcaa atgaaaaggc cctgccatga aaaaactatt
    14221 cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga cttatttttt
    14281 tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg
    14341 atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc
    14401 aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag
    14461 ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct
    14521 ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa
    14581 ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca
    14641 taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg
    14701 ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact
    14761 tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata
    14821 aataccatga agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca
    14881 atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct
    14941 acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct
    15001 tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc
    15061 ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca
    15121 gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg
    15181 aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat
    15241 acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc
    15301 cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta
    15361 ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac
    15421 atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt
    15481 acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt
    15541 gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg
    15601 actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata
    15661 aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac tctccttgat
    15721 tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc
    15781 gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa gacaacctca
    15841 aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag
    15901 aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg
    15961 ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt
    16021 cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca
    16081 atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa
    16141 attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact
    16201 cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt
    16261 tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga
    16321 aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt
    16381 cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat
    16441 atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc
    16501 ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt
    16561 caaatacata tgtcgcaaag taagagataa cgtttctcat gcggatatct ttgatgacga
    16621 taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg
    16681 cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg
    16741 taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt
    16801 cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga
    16861 agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga
    16921 ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag
    16981 ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa
    17041 ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg
    17101 aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac
    17161 gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga
    17221 tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg
    17281 tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta
    17341 agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt
    17401 ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg
    17461 caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg
    17521 tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac
    17581 gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac
    17641 tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac
    17701 gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct
    17761 cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca
    17821 tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct
    17881 tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta
    17941 tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac
    18001 tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat
    18061 tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat
    18121 tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa
    18181 ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat
    18241 agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac
    18301 aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa
    18361 tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt
    18421 tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc
    18481 caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc
    18541 gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc
    18601 ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac
    18661 ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt
    18721 taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta atccagctgt
    18781 gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag
    18841 atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg agattcctac
    18901 ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc
    18961 gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa
    19021 tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt
    19081 atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc
    19141 gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct
    19201 tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac
    19261 gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt
    19321 ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc
    19381 cgtgtagttt ttttatgaaa gcaggaacct gctagcatat gtaattaatt atttcttcag
    19441 gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg
    19501 taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc
    19561 gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg
    19621 cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc
    19681 ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag
    19741 gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt
    19801 tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag
    19861 aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa
    19921 aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc
    19981 acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct
    20041 acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca
    20101 gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac
    20161 caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt
    20221 cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc
    20281 tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag
    20341 gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa
    20401 ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa agacgaacaa
    20461 ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca
    20521 caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc
    20581 gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt
    20641 gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat
    20701 cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca
    20761 tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa
    20821 gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg
    20881 aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa
    20941 gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc
    21001 gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc
    21061 gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc
    21121 tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa
    21181 aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa
    21241 tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt
    21301 ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc
    21361 cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc
    21421 attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct
    21481 ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc
    21541 atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa
    21601 aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt
    21661 tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata
    21721 aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc
    21781 gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg
    21841 tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc
    21901 ttttcaaaac cgttcatttg tagagcctcc cgtaatt
//
LOCUS       DAFGFZ010000048        20644 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000048 DAFGFZ010000000
VERSION     DAFGFZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.048"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            34..402
                     /locus_tag="LR726_001490"
     CDS             34..402
                     /locus_tag="LR726_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL6216590.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            572..1003
                     /gene="mraZ"
                     /locus_tag="LR726_001491"
     CDS             572..1003
                     /gene="mraZ"
                     /locus_tag="LR726_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL6216591.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1024..1983
                     /gene="rsmH"
                     /locus_tag="LR726_001492"
     CDS             1024..1983
                     /gene="rsmH"
                     /locus_tag="LR726_001492"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL6216592.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1988..2389
                     /gene="ftsL"
                     /locus_tag="LR726_001493"
     CDS             1988..2389
                     /gene="ftsL"
                     /locus_tag="LR726_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL6216593.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2390..4582
                     /locus_tag="LR726_001494"
     CDS             2390..4582
                     /locus_tag="LR726_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL6216594.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4608..5570
                     /locus_tag="LR726_001495"
     CDS             4608..5570
                     /locus_tag="LR726_001495"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL6216595.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5584..6945
                     /locus_tag="LR726_001496"
     CDS             5584..6945
                     /locus_tag="LR726_001496"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL6216596.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6971..8059
                     /gene="murG"
                     /locus_tag="LR726_001497"
     CDS             6971..8059
                     /gene="murG"
                     /locus_tag="LR726_001497"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL6216597.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8074..9294
                     /locus_tag="LR726_001498"
     CDS             8074..9294
                     /locus_tag="LR726_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL6216598.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9450..10775
                     /gene="ftsA"
                     /locus_tag="LR726_001499"
     CDS             9450..10775
                     /gene="ftsA"
                     /locus_tag="LR726_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL6216599.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10798..12039
                     /gene="ftsZ"
                     /locus_tag="LR726_001500"
     CDS             10798..12039
                     /gene="ftsZ"
                     /locus_tag="LR726_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL6216600.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12056..12733
                     /locus_tag="LR726_001501"
     CDS             12056..12733
                     /locus_tag="LR726_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL6216601.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12747..13346
                     /locus_tag="LR726_001502"
     CDS             12747..13346
                     /locus_tag="LR726_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL6216602.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13361..13648
                     /locus_tag="LR726_001503"
     CDS             13361..13648
                     /locus_tag="LR726_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL6216603.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13697..14479
                     /locus_tag="LR726_001504"
     CDS             13697..14479
                     /locus_tag="LR726_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL6216604.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14500..15207
                     /locus_tag="LR726_001505"
     CDS             14500..15207
                     /locus_tag="LR726_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL6216605.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15469..18267
                     /gene="ileS"
                     /locus_tag="LR726_001506"
     CDS             15469..18267
                     /gene="ileS"
                     /locus_tag="LR726_001506"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL6216606.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEERNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18316..19842)
                     /locus_tag="LR726_001507"
     CDS             complement(18316..19842)
                     /locus_tag="LR726_001507"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL6216607.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19857..20504)
                     /locus_tag="LR726_001508"
     CDS             complement(19857..20504)
                     /locus_tag="LR726_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL6216608.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6981 a         3572 c         4416 g         5675 t
ORIGIN      
        1 cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg
       61 ttttggtata tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt
      121 tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc
      181 ggtatgcatg cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt
      241 tctgttttgc taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt
      301 atttatccgc gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg
      361 tacgctgtct tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt
      421 aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa
      481 aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa
      541 ttttagttag tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat
      601 tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt
      661 tgttctgaca cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa
      721 tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg
      781 attcttctat tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc
      841 cagcacgtta agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa
      901 ccgaattgaa atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa
      961 ctttgacgaa attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga
     1021 ctaatgactg aaacatttca acactataca gtcatgttga aagaaacggt agatggattg
     1081 aacatcaaac cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa
     1141 tatttattaa gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa
     1201 gcaatcgatc acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt
     1261 atcaaagcga acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac
     1321 ggcattttgt atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt
     1381 agttatcatc aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct
     1441 tatcacgtaa ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc
     1501 gaggagaaat tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca
     1561 atccaaacaa ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga
     1621 agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac
     1681 gaactcggtg ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga
     1741 agaatcagcg taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa
     1801 gaatatagta cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa
     1861 ccagaactga aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa
     1921 aacaatcgat cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag
     1981 tagaatgatg gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt
     2041 ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca
     2101 atcaccggca aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt
     2161 ggcgattgct attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg
     2221 agctgaaaaa gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac
     2281 gagtttagaa caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga
     2341 agaaaaagga ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa
     2401 aaataaaatt cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa
     2461 agtaggtatc attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt
     2521 ctcgtatatc gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca
     2581 gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg
     2641 tgtggcgctc gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata
     2701 tacctctggg gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat
     2761 cctgcataag aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc
     2821 aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac
     2881 caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga
     2941 ccatcaggct cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt
     3001 tccagacaaa gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga
     3061 tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct
     3121 gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt
     3181 agacagcaga ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca
     3241 gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg
     3301 acaacgacct actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg
     3361 gcgtaatttc ttggtacaag atagttatga accaggatca accatgaaag ttttcactac
     3421 tgcagctgct atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat
     3481 ccaagtagaa gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat
     3541 gcgtcaagct ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg
     3601 cggacgatgg tataactatt tacaaaaact gggatttgga caaagtactc attctggatt
     3661 agatgatgaa gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc
     3721 ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc
     3781 gaataacggt acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg
     3841 agaagagcga acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga
     3901 aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg
     3961 tgtatatagt gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc
     4021 tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt
     4081 tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga
     4141 tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga
     4201 aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg
     4261 taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt
     4321 gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc
     4381 tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg
     4441 gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg
     4501 agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact
     4561 tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac
     4621 aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta
     4681 ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc
     4741 acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt
     4801 tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc
     4861 tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca
     4921 agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa
     4981 tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac
     5041 tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt
     5101 caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct
     5161 cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt
     5221 gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga
     5281 tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa
     5341 tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa
     5401 gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc
     5461 caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt
     5521 ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg
     5581 gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa
     5641 agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat
     5701 ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta
     5761 ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat
     5821 ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta
     5881 attacggaag ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga
     5941 acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat
     6001 caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa
     6061 gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca
     6121 gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat
     6181 ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa
     6241 gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa
     6301 gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat
     6361 tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt
     6421 aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca
     6481 aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat
     6541 gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg
     6601 actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta
     6661 gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc
     6721 gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca
     6781 atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat
     6841 ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa
     6901 actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga
     6961 gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc
     7021 tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg
     7081 agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt
     7141 ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt
     7201 tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat
     7261 cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc
     7321 aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata
     7381 tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc
     7441 agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt
     7501 gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg
     7561 agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta
     7621 ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc
     7681 tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc
     7741 cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg
     7801 gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc
     7861 aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga
     7921 atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg
     7981 ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa
     8041 acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa
     8101 ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa
     8161 tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat
     8221 acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc
     8281 cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca
     8341 tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta
     8401 tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat
     8461 tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac
     8521 agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat
     8581 tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca
     8641 aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt
     8701 gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat
     8761 gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca
     8821 aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt
     8881 tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat
     8941 gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg
     9001 caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg
     9061 catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa
     9121 agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca
     9181 caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca
     9241 agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac
     9301 aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc
     9361 agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat
     9421 ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg
     9481 atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata
     9541 ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg
     9601 ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc
     9661 agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg
     9721 gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg
     9781 cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac
     9841 aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa
     9901 gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta
     9961 aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga
    10021 ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg
    10081 gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc
    10141 aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca
    10201 atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg
    10261 aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact
    10321 tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg
    10381 accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc
    10441 ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc
    10501 caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt
    10561 cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag
    10621 cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac
    10681 ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata
    10741 aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga ataagatatg
    10801 gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt
    10861 ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc
    10921 attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa
    10981 ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa
    11041 gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc
    11101 atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc
    11161 gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga
    11221 ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca
    11281 ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt
    11341 gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg
    11401 atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac
    11461 caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca
    11521 acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta
    11581 cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat
    11641 atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa
    11701 gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag
    11761 gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact
    11821 gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat
    11881 tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat
    11941 atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat
    12001 gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat
    12061 tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg
    12121 tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga
    12181 agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga
    12241 aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca
    12301 gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt
    12361 aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt
    12421 aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt
    12481 ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa
    12541 agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct
    12601 tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca
    12661 agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt
    12721 caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt
    12781 ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa
    12841 aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg
    12901 ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta
    12961 ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag
    13021 cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa
    13081 tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg
    13141 ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg
    13201 ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg
    13261 agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg
    13321 cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt
    13381 acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt
    13441 gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg
    13501 cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac
    13561 aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat
    13621 atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag
    13681 ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac
    13741 atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct
    13801 gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag
    13861 tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat
    13921 tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca
    13981 ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac
    14041 ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt
    14101 tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc
    14161 tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca
    14221 agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat
    14281 ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga
    14341 aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg
    14401 cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt
    14461 cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat
    14521 atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat
    14581 ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa
    14641 aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg
    14701 aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag
    14761 gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct
    14821 gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca
    14881 gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca
    14941 cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt
    15001 ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg
    15061 atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac
    15121 acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc
    15181 gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag
    15241 ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct
    15301 gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt
    15361 gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg
    15421 aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa
    15481 gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa
    15541 ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac
    15601 gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat
    15661 ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct
    15721 ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta
    15781 ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt
    15841 cgtgaatatg cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt
    15901 gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta
    15961 cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac
    16021 tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa
    16081 tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat
    16141 acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct
    16201 gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg
    16261 aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa
    16321 gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa
    16381 tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact
    16441 gctccaggac atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg
    16501 tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt
    16561 tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag
    16621 ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat
    16681 cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa
    16741 gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt
    16801 gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc
    16861 tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta
    16921 tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa
    16981 ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat
    17041 gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca
    17101 ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt
    17161 atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga
    17221 tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca
    17281 gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat
    17341 tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt
    17401 aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa
    17461 gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac
    17521 caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac
    17581 cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa
    17641 gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc
    17701 tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa
    17761 gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt
    17821 tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc
    17881 cgtgataatg tcttaaaagc attggaagaa gaaagaaaca gcaaattgat tggtaaatct
    17941 ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg
    18001 gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca
    18061 gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa
    18121 gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa
    18181 aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct
    18241 gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca
    18301 cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg
    18361 ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga
    18421 tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc
    18481 cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt
    18541 caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact
    18601 atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt
    18661 caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag
    18721 tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt
    18781 gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa
    18841 aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt
    18901 ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa
    18961 ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat
    19021 gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac
    19081 tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt
    19141 aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa
    19201 agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc
    19261 aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa
    19321 aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta
    19381 atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt
    19441 ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata
    19501 gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt
    19561 ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt
    19621 agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta
    19681 gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt
    19741 tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt
    19801 accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat
    19861 attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca
    19921 ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg
    19981 atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg
    20041 atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca
    20101 acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag
    20161 ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca
    20221 tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt
    20281 ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa
    20341 ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca
    20401 gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct
    20461 agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc
    20521 atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta
    20581 aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag
    20641 actc
//
LOCUS       DAFGFZ010000049        20364 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000049 DAFGFZ010000000
VERSION     DAFGFZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.049"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            293..1432
                     /locus_tag="LR726_001509"
     CDS             293..1432
                     /locus_tag="LR726_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL6216609.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1499..2074)
                     /locus_tag="LR726_001510"
     CDS             complement(1499..2074)
                     /locus_tag="LR726_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL6216610.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2079..3053)
                     /locus_tag="LR726_001511"
     CDS             complement(2079..3053)
                     /locus_tag="LR726_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL6216611.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3077..3121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3336..4304
                     /locus_tag="LR726_001512"
     CDS             3336..4304
                     /locus_tag="LR726_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6216612.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4417..4695
                     /locus_tag="LR726_001513"
     CDS             4417..4695
                     /locus_tag="LR726_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL6216613.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4813..5049
                     /gene="secG"
                     /locus_tag="LR726_001514"
     CDS             4813..5049
                     /gene="secG"
                     /locus_tag="LR726_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL6216614.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5152..5907
                     /locus_tag="LR726_001515"
     CDS             5152..5907
                     /locus_tag="LR726_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6216615.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5910..8267
                     /gene="rnr"
                     /locus_tag="LR726_001516"
     CDS             5910..8267
                     /gene="rnr"
                     /locus_tag="LR726_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL6216616.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8289..8753
                     /gene="smpB"
                     /locus_tag="LR726_001517"
     CDS             8289..8753
                     /gene="smpB"
                     /locus_tag="LR726_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL6216617.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9055..9774
                     /gene="atpB"
                     /locus_tag="LR726_001518"
     CDS             9055..9774
                     /gene="atpB"
                     /locus_tag="LR726_001518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL6216618.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9827..10042
                     /gene="atpE"
                     /locus_tag="LR726_001519"
     CDS             9827..10042
                     /gene="atpE"
                     /locus_tag="LR726_001519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL6216619.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10133..10657
                     /gene="atpF"
                     /locus_tag="LR726_001520"
     CDS             10133..10657
                     /gene="atpF"
                     /locus_tag="LR726_001520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL6216620.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10644..11186
                     /locus_tag="LR726_001521"
     CDS             10644..11186
                     /locus_tag="LR726_001521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL6216621.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11209..12765
                     /locus_tag="LR726_001522"
     CDS             11209..12765
                     /locus_tag="LR726_001522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL6216622.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12779..13681
                     /locus_tag="LR726_001523"
     CDS             12779..13681
                     /locus_tag="LR726_001523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL6216623.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13704..15110
                     /gene="atpD"
                     /locus_tag="LR726_001524"
     CDS             13704..15110
                     /gene="atpD"
                     /locus_tag="LR726_001524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL6216624.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15124..15546
                     /locus_tag="LR726_001525"
     CDS             15124..15546
                     /locus_tag="LR726_001525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL6216625.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16015..16245
                     /locus_tag="LR726_001526"
     CDS             16015..16245
                     /locus_tag="LR726_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL6216626.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16342..17643
                     /gene="murA"
                     /locus_tag="LR726_001527"
     CDS             16342..17643
                     /gene="murA"
                     /locus_tag="LR726_001527"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL6216627.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17656..17832
                     /locus_tag="LR726_001528"
     CDS             17656..17832
                     /locus_tag="LR726_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL6216628.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17967..18263
                     /gene="yidD"
                     /locus_tag="LR726_001529"
     CDS             17967..18263
                     /gene="yidD"
                     /locus_tag="LR726_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL6216629.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18327..18710
                     /locus_tag="LR726_001530"
     CDS             18327..18710
                     /locus_tag="LR726_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6216630.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18879..19763
                     /locus_tag="LR726_001531"
     CDS             18879..19763
                     /locus_tag="LR726_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL6216631.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19877..20113
                     /locus_tag="LR726_001532"
     CDS             19877..20113
                     /locus_tag="LR726_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL6216632.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20201..20281
                     /locus_tag="LR726_001533"
     tRNA            20201..20281
                     /locus_tag="LR726_001533"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20235..20237,aa:Tyr,seq:gta)
     gene            20288..20359
                     /locus_tag="LR726_001534"
     tRNA            20288..20359
                     /locus_tag="LR726_001534"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20320..20322,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4426 g         5611 t
ORIGIN      
        1 gtttttttgc aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca
       61 tccttgaaaa aggaaagcta acaataacta gcatcatata agagctttta aggatagaac
      121 agtagaatag gaatctatgg gcaagtcctc tcgtataaat aaaataggag ggaagtagca
      181 gcatattttt cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa
      241 aattcgagca cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa
      301 agaagaaaaa atcgcgattt tacaagagat tatccgaatt aaatcagtga atggaaacga
      361 aggtgaagta gcagcctatt tgaataagct tttggcaagg catgacataa caggagagat
      421 tgtttcttac agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg
      481 aaaagtgtta ggcctatctg gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg
      541 gacctatgca ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac
      601 agatatgaag agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg
      661 aaaaccattt aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact
      721 aggtggggaa cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg
      781 agagccaacc aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac
      841 ctcgcacgga aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca
      901 cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga
      961 gaatcctgtc ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt
     1021 caacagcatc ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc
     1081 aaatgataaa atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga
     1141 ctatcatttg gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc
     1201 tcctttgatt cattccattc agcaacaatt cagccagcca ttacctttag ttggagcggc
     1261 agcaacaacg gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt
     1321 tgggcctggt gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta
     1381 tttggatatg atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt
     1441 taataaatga aaaaacagag ccagattagc attattttgc taatccagct ctgtttattc
     1501 agcctatttt tctttttgaa tatatcaagc gttggggcaa catagtgatg actaatgaaa
     1561 caacgacgat agacaacaag cgatcaaggt agtctgtacc cacctgtacc aagatcacac
     1621 ttgttgtctg acttaaacct aacccatgta atagctgaac gatcatactg gatcctgagg
     1681 aggtgatccc gccaaataac tgaacggtaa tgatcgaaga aacgatcgtt ccaggtaccg
     1741 acaaaatcaa agcgagtaat aacagtttca agctgttttt atctttgccg agatatcttg
     1801 gcaaaatcaa tccagcaagc aagcctgtga gaatttggat tggcgaataa tacagagaaa
     1861 aaatatctgt agtcactcca cttaaaatac cgctggacaa tcctgtcagc attccaggta
     1921 caggcccaag caatgcacca gcaaaaatcg ttccgataga atcgagatag attggcaaac
     1981 gaagccataa agcaatcgtt cctccaatat aattgatccc aatacacaaa gccatcactg
     2041 taagtacgcg agttgccatt ttgtttttat tcattttgtt agcccatctt taacctttcc
     2101 aaatctttca taatcgtttc ttcttgtgca tttaaaagaa cggttaaaaa ctttcggaaa
     2161 aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg ccaaaattca
     2221 taacgatcaa ccaatgtttg tccgcgactt attccttgag tttctacagc agtgaagctt
     2281 ttaaagcctt cacagatgga ctcatcgata aaataagcaa cagctaatgg atcattgatg
     2341 acacatccta agattctttc ttgtttccag tgaaagtcaa aataaaaacg agtgatcgct
     2401 tttagatact ctccctcttc aggattcatt tggcagcaat attcaaggat agtcggtgtc
     2461 aagacgattt ctcttgtcac atctaaccca accatttcta tcggttgttt gagcttttcg
     2521 aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac tggtgaacag
     2581 ttaccatggc ttttgtaagt tccgcccatt gagacaaaac gatccatatg tttcccaaca
     2641 gatgggctgt ttttcaaagc agaagcgata ttggtcaaag gccccaaagc aatgactgat
     2701 gtatctgttt tttctttgaa ataatcagtc agaaaatcaa ctgcatggag cgattctgcc
     2761 tgtttggcta agaccatcgg gaaatttgtg tcccctaatc catccaaccc atgagtgtct
     2821 tgcgcactaa tgaaaggttg cttcaaaggt ttttccattc cttgataaac agggatatct
     2881 aaacgatcac agcgctccaa acatttcagc acattctgcg taccgatatc tacagggaca
     2941 ttcccgcaaa cgacggtaat agcaactact tcgatatcag gggatttgac agctagtgat
     3001 aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttactttacg cattatttcc
     3061 aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga accagtccaa
     3121 gacgagtgtc ttgtttataa tgcacgaaat tagtatactg gcttttattg tgaatgtaaa
     3181 tgctctctta caagtaaatc tattgagaag aactttaagt aaatgataca atgaaaaaga
     3241 aatggaattt acgtgtgccg aagaaaaaga atcctattga tcggtatatt gtataataaa
     3301 tagattatag tttggcagaa agcggcgggt gtacgatggt tattttttat ttcattctta
     3361 ttctgatatt tataggaatc agttaccatt ttctttctta tttttatgca aagaaattag
     3421 taagagcggg ggtacagaga ggctatgact ggtatgaaga aactgggcac cgcttgatgg
     3481 atcctgctgc agtcacgtca gtagaaaaaa agcgacaaaa attagaagaa gagtgtgaaa
     3541 gcttctggga acaaggaata ccgaaaacga tcaaaagtta tgacgggttg aaactagctg
     3601 gacagatgtt cctccaacca actcagcaga ataaatgggt catttgtgta catgattatc
     3661 gaagtacagg gaaaagagat atgtctcata taggtaaaag atatgcagag aaaggattca
     3721 acgttcttat acccgattta cgtgcgcatg gagaaagtga aggagaaatc atcggaatgg
     3781 gatggctaga ccggctagat ttaattgcat ggatccaact catcttagat gaacaaccag
     3841 atgcatccat tattttgcac ggcagttcga tgggcgcttc tactattatg atggcaagtg
     3901 gtgaaaagct tccatctgca gtcaaaggat ttatcttaga cagcggatat gtttctgttt
     3961 atgcggaatt tcggtatatg ttaagtaaaa tcacggtctt tcctaaaaaa atgatcatga
     4021 gatatgcgaa tcactatgct cagaaatacg caggttattc tttaaagcaa gcatcagcta
     4081 caagacagct tggtagtaac catttgcctt tgttagtcat ccatggtgaa agagatcatt
     4141 ttgttcctac agaagcagca tacacgattc aaaacgctac ggcaggggat aaagcattac
     4201 tgcttgttcc agaagcagaa catttggaag catcgatgaa agatccgaaa acctattgga
     4261 cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttgattttct cttccttttt
     4321 gtaagcaaca cgaggataaa taattgttta tagaccatga gactgttaaa ataaggtagg
     4381 aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga ggaaaaaaat
     4441 cgtcttgctt tattcaatga tgagcaacaa gaaatcggag aaatgacatg gtcggatgcg
     4501 gggccagaca tcatgatcat tgaccataca tttgtcgatc cagcatatcg tggtcaaaaa
     4561 ttagctgaaa agctagtgta tactggagta gaactagcaa gacgagaggg gaaaaagatt
     4621 atcccttcat gcccttacgc aaaaaaagag tttgagaaga aaccagagta tcatgacgtc
     4681 ttacgccaat cataataata acagctgaaa gatttgcact ttcagctgtt tttccattta
     4741 tatagagttg tcacggagtt ttatctatga taaagttaaa gagacgatat cttttgggaa
     4801 ggaggctgct atatgtataa tttaattttg ggaatcgtga tcgttttatc gattgttatg
     4861 gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc atttaccggc
     4921 ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt catgcagcga
     4981 tcaactgcag tcatgggagc tgtctggatg atcttattat ttgtcctagc atttttatct
     5041 tcgaagtagt cgaataagcc tctcgcacaa cggcgggcgg ctttttttat tgcaaagaga
     5101 acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga ggtgaaacga
     5161 gtgaaacaat taccaaaacc cctttatgca aaacatggaa aacgagcagt tttgctgctt
     5221 catgcgtact caggaagtcc aaatgatgtc cggatgctcg cacggttttt agaaaaagca
     5281 gattatacag tctatgcgcc gctatttaaa ggacatggaa cgatggagcc atatgatatc
     5341 ttgcaagaaa aagcagaaag ctggtgggca gatactaaaa aagctatcca ttttttacag
     5401 tctgaacagt tttctgatat tgccgtattg ggattatcga tgggtggcat ctttgctgta
     5461 cgggcactag aagaagaatc agtaattggc ggcggttttt tctgttcgcc cctgtctcct
     5521 gtagaaacaa acgtgcctga aaattttgaa aaatatgtac gtcaagtgct gaagatagca
     5581 ggcaaatcag aaaaagaaat caatgaaaaa gctgtgtctt accgctcgtt agctgaacat
     5641 caattaatgg atatacaaga ccaagcagca atcgcagaaa gtagactatc agatatccaa
     5701 cagccaatat ttttagctca agctgggaaa gatgaaatga tcgacccaaa tggtgtattt
     5761 gaaacggcac gcaaattgag ccgccaacga gtgacacttc aatggtatcc tgaaagcggt
     5821 catgtgatta cagttggcac agcaagacgc gaattagaaa aagacgtgtt ggaattttta
     5881 gaaaaattac cttggaatga ggaataaata tgacaaaaga aacaataaaa gagaaaattc
     5941 tctttttcat ggaaagcagt cgcaaacgta gtttttctat ggaagaaatc gctgaaggtc
     6001 taggctttca aaaaagcgat gattttaaat tactagtaca aacgattgct caaatggaaa
     6061 gagaacagag tatcgttttc aataaaaaag gaaaagtcaa acttccttta aaaccgatct
     6121 tggtcgaagg gacatttcgg gcaaatgaac gaggatttgg atttgtaacg attgatcccg
     6181 aagaagatga tatctatatc ccgaaagaag cgactggctt tgcaatggat ggggataccg
     6241 tagcaattga tatcatgaag cgagcaaata cagatatgga tcgcggagca gagggaaaag
     6301 tagtggagat tcgagaccga gcgactactc agttagccgg agaatttgtt gcttattcag
     6361 acgaagagat acaggaaacg gatctttatg gtgtggtcat cccgaaagat agaaagctta
     6421 accaattcaa agtgtatgca gcggcagagg gagttcgtcc agttgacgga agtattgtat
     6481 tgatcgaact gacccattac cctgaaaaag ggtatgcaac tagtttagaa gggataatca
     6541 agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcgatt gtcgtggcta
     6601 acggtattcc tacaaaattt cctgatgatg tgttatctga ggcagatgaa gttccagacc
     6661 agatcagcga aaaggatatt gttggacgca gagatttaag agatcagctc atcgttacga
     6721 ttgatggaga agatgcaaaa gatctagatg atgcagtgac ggtcaaaaaa ttagaaaatg
     6781 gtaattattt tcttggagta cacattgcag atgtatctta ctatgtgacg caaggcagtc
     6841 aactggacag agaagcatac gaaagaggaa caagtgtcta tttgaccgat cgagtgattc
     6901 cgatgatccc acaacgttta tccaatggta tctgttcatt gaatccccat gttccacgac
     6961 tcaccatgag ttgtgaaatg gagatcgatc caaatggaca agttgtctct catgagattt
     7021 ttccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat caaatcttag
     7081 aagatcaaga tgaagaaacg atgcaacgtt atgctcagct cgtgcctatg ttccaagaaa
     7141 tgggtgaatt gcatcgaatc cttgaaatga tgcgaatcaa acgtggagcg atttcttttg
     7201 aggatcgtga agcgaagatc cttgttgatg ctgaaggaca cccaaatgat atccagctaa
     7261 gaagcagagg gataggtgaa cgcttgatcg aatcctttat gcttgctgcc aacgaaacgg
     7321 tagctgaaca ttataataaa ttaaatcttc cgtttattta tcgaatccat gaacagccaa
     7381 aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt ctggtccgag
     7441 gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga gtagaagaca
     7501 aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa gcacgctatt
     7561 cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac tttacatctc
     7621 ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac agtgaagata
     7681 aaagtgaaag caatcaaagt tcgtgggctg cggaacttcc tgaaattgca gatcatagtt
     7741 ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg aagaaagcag
     7801 aattcatgat ggacaaagtg ggagaagaat tcgatgggat catcagctct gttgtgaaat
     7861 tcggcctgtt tatcgaactg ccgaatacag tagaaggatt gatccatatc aatgaattga
     7921 aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa agaactggtc
     7981 tgacttttaa aatcggacaa aaagtgcgcg taaaagtagt caaagctgat ccagatgaac
     8041 gagcagtgga ttttgaactc gtttccgcag aagaggtcac tgcgttggaa cgtccaaaag
     8101 cacgcaatgg aaaatctgga aaacgagaag gaaaacaagc aggcaaacgc tcagaaaaaa
     8161 atggcaagaa gaacaaacga tatagtgata gtaagcaaac gttcggtaag aaaaaaggga
     8221 aaaagccatt ttacaaaggt gtcaaaaaga agaaaaagaa aaaataaaat gagttggagg
     8281 aaacagctat gccaaaaggc gagggaaaac taattgcaca aaacaagaaa gctcgccatg
     8341 attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacg gagatcaagt
     8401 cgatacgaaa cagccggatc aatctgaaag atggatttat tcgcgtccgc aacggggaag
     8461 ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt aatcatgatc
     8521 cgttgcgcac gagaaagtta ttattacaca agaaacaaat cgttcgtctt gaaaacgaat
     8581 tgaaaaacac tggaatcact gttgttcctt taaaagtcta tattcgtaac ggctatgcca
     8641 aggtattgat tggtctggca aaagggaaaa aatcgtatga taaacgggaa gatttgaaac
     8701 gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga taatttcatt
     8761 atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt gttagcattt
     8821 aatctgataa aacatgatta tcacaggttt tttcttagat caggagcacc gtgcatgggg
     8881 aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg aaaatgtttt
     8941 gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg gaaaaaacca
     9001 gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa tggattggat
     9061 gaacgctcgc taacgtttca cattggacca atttggtttg atggtacagt ttgtataatg
     9121 gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag aaatcttaag
     9181 ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag tcgaggaatc
     9241 gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc atttacattg
     9301 tttttatttg tttttgttgc aaacaacata ggtttagtaa caaaaatcgt cttacctaac
     9361 gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac tttggcattt
     9421 ataatgatct tacttactca cttattcggg gtgaaaaagt taggcttcaa aggatatttc
     9481 gtaaattcgt tcttgaaacc ctatagtttt atgtttccga tgaaactgat tgaagaattt
     9541 acaaacttat taacacttgc tttacgtctt tatgggaata tttatgcggg ggaagttttg
     9601 ctaaccttga ttgcaaaaat gatgacaagc ttaggctggt tctcattacc attagcaatt
     9661 ccattagaga tggtctggat cgcgttctcc ttgtttattg ggagcatcca agcatttgtc
     9721 tttgtcactt tgtcaatggt ttatatgagc cataagattg aagtagaaga ataatactcg
     9781 gttaaaaaac aaactactat ttatctattt taggaggaaa acaattatga attatatcgc
     9841 agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg gtcaagttat
     9901 ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat tccgtacaac
     9961 aatgtttatc ggggtagctc tagttgaagc tgttcctatc ttaggggtag ttatcgcctt
    10021 gattctagtt tttgccgttt aattgaatca gtattaaggc agaaacaaca tcgtttgtta
    10081 ctgctttttt acatggaaac tagaaatact ccaaagaaag gaaggctcgc ctatgctgaa
    10141 tcaattggca attgcagaag ttggcaatgc gatgttaggt aatatcatcg ttgtcagcgg
    10201 ctcatttttg atactactgg ctctcttgaa gcactttgct tggggaccaa tcagcgatat
    10261 tttgaaaaaa cgtgaagaca agatcgccaa tgatttagat tctgcagaac aatctcgcat
    10321 caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc gttctgatgc
    10381 agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa atattttgaa
    10441 ggatgctcaa gaagaagcag ctcgtctaaa aagcaaagcc caagctgata tcactgtaga
    10501 acgtgattca gcgctgaact ctgtaaaaga cgacgttgca gaactctctc ttcaaatcgc
    10561 ggaaaaaatc ttgaacaaag aattatctcc agaaatgcat gaatcattaa tcaatcaata
    10621 cattgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga agacgttacg
    10681 ggaaagcttt atttgaactg gcgatcgatt caaatcaagc agaagaaatc tatcaagatc
    10741 tcgtaagttt acgtcaaatc tatgaacaag tacctggatt agggaatatg ttaagtgatg
    10801 ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgtcagc ggttatgatg
    10861 gaatcgttaa aaacttttta gaagttgttt atgagtataa ccgaatggat gaccttttgt
    10921 tcatgattga agaatatgaa cgtcgatatg atgaacatca agggttacta ttgggaagtg
    10981 tcacaacagc agtcccactt tcagacgaac aacgaataca attagaaaag aatgttgcaa
    11041 aaacaatgga ctatcaatca gttgaattaa aacaaatcgt tgattcttct attattggtg
    11101 gcgcgattgt tgaagccaac catcgagtaa tcgacggaag cattcgcacg caattagaaa
    11161 aaatgcgtaa tcaattaaac agatagtaca aagaggtagg tgaatcgaat ggccatcaaa
    11221 gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa cgtgctttca
    11281 gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc acatggttta
    11341 gaaaacgcga tgagtggtga gttgcttgaa ttttcaaacg gctcatacgg aatggcgcaa
    11401 aacttagagt caaatgatgt aggtatcatt atccttggcg atttcgagtc cattcgtgaa
    11461 ggagataaag taaaacgaac aggtaagatc atggaagttc cagttggaga ggccttgatc
    11521 ggtcgggtag tcaatccgct aggtcaacca atcgacggac taggtgaaat cgttactgac
    11581 aaagctcgtc cagtagaagc gatggcacca ggtgttatgc aacgtaaatc tgttaacgaa
    11641 ccaatgcaaa cagggctaaa agcgatcgac gcccttgtgc caatcggacg cggacaacgt
    11701 gaattagtca tcggtgaccg taaaacaggg aaaacttcta ttgcgattga tacgatcatc
    11761 aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa agattctaca
    11821 gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac aatcgttgtg
    11881 aatgccggtg cgtctcaacc agcaccatta ctttatatcg caccatatgc tggtactgca
    11941 atgggtgaag aattcatgta caacggtaaa catgtattga tcatttttga tgatctttca
    12001 aaacaagctg ttgcttatcg tgaactttcc ttgctacttc gccgtccacc aggtcgtgaa
    12061 gcttatcctg gggatgtttt ctacttgcac tcacgtttat tagaacgtgc agcaaaatta
    12121 agtgatgatc taggcggcgg ttcaatgact gctttgccat ttgttgaaac acaagctgga
    12181 gatatctctg cttatatccc aacaaacgtt atctcaatca ctgacggaca aatcttctta
    12241 gaaagcgact tgttctacgc gggtactcgt ccagcggttg atgccggact atctgtctct
    12301 cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac tttacgtcta
    12361 gacttagcaa gttatcgtga attagaagca tttacacaat tcggttctga tttggatgcg
    12421 gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa acaaaaactg
    12481 catgcaccgc tagctgttga gaaacaagta gtgattttgt atgccttgac acatggattc
    12541 ttggacagta tccctgtaga cagtatttta gacttcgaac atgaattatt cgaatatctg
    12601 gataccaatc attcagatat ttttgaaaca atccgtacaa caaaagatct tccagaagaa
    12661 gaaacgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac aaaaggaaat
    12721 aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg tgagatgaat
    12781 gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa cgagccaaat
    12841 cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag cttcttcaca
    12901 gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga ctgctactca
    12961 gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca gcatgctgct
    13021 cagccgtcct gtgaagaaaa ccggttatat cgtcatcact gcagatggcg gtctggtcgg
    13081 tggatataac agttcgatat tgaaacaaac gatgtctatt ttagaagaag accaccaatc
    13141 acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct ttaaagcacg
    13201 cggtatcaac ctagcatatg agctgcgcaa tttatctgat cagccaagct ttgatgaagt
    13261 ccgcaaaatc gtaaatatgg caacaactat gtaccaaaac gaagtattcg atgaattata
    13321 cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag aaaaaatgct
    13381 gccaatctca gacttagatc cagaagaagc aacgactttc gaacaagaat atatttttga
    13441 accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa gtttgatcta
    13501 cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag caatgaaaac
    13561 agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttata accgtgcaag
    13621 acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg cactagaata
    13681 gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta atcggtcccg
    13741 ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac gcattagttg
    13801 tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta gaattagggg
    13861 acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga ggaatggaag
    13921 tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta ggtcgcgtat
    13981 tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat gcggaaagaa
    14041 gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca gaaattttgg
    14101 aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg aaagtcggac
    14161 tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc cataatatcg
    14221 cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact cgtgaaggta
    14281 atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc atggtgttcg
    14341 gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt ttgacgattg
    14401 cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac aacattttcc
    14461 gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct tctgcggttg
    14521 gttatcaacc aacattggca acagaaatgg gtcaattgca agaacgtatc acatctacga
    14581 aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac tacactgacc
    14641 cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa cgtaaattaa
    14701 cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt gctttagcac
    14761 ctgaaatcgt gggagaagaa cattataaag tagcaacaga agtccaacac gtattgcaac
    14821 gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta tccgatcaag
    14881 aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa aactttaacg
    14941 tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa acagtgaaag
    15001 gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct ttccgaagtg
    15061 ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag aaaggggtgc
    15121 cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt ctatgatcac
    15181 catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt gcctaaacat
    15241 gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac agattcagat
    15301 acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga taatgttgtt
    15361 tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg tgcggaacgt
    15421 gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga tacgaacgaa
    15481 ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt atctaagcac
    15541 tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga ggttatgaca
    15601 gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc aaatttttgt
    15661 gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc agaggttgtg
    15721 aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc tcatattttg
    15781 ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt ggaataaggg
    15841 actgcgacaa gacttttgtc tcagtccctt tttttgcata tttttagcat ttatttccta
    15901 aaaaatgtaa ggggattcct atatttttaa gtatacttgt ttttcctttt atacatttta
    15961 tggtattatc attttattag ttattataaa acaatcgtta gaaaggacgt cattatgcaa
    16021 gtatacggaa ttgatgcggt gcttcgaatc gtgagtcatt tggcgtttat ctacttagcc
    16081 ttctggtctt tacgttcgtt acgcattgaa acttttttca gaccaatgca taatacgcaa
    16141 attagaatgg caatcgtact tttttcaatt tttttagggt acactgcaag taattttttc
    16201 ttagaagtta tcgctttatg ccgaaatctt ttcgttagcc tgtaaatttt aaaaaaaggt
    16261 actattttaa aagcaaggat gttataatag aaaagatttt gagtaagtgt atgctcattg
    16321 gaattttttg gagggaacaa catggaagag atcatcgtaa gaggtggcaa tcaattaaat
    16381 ggaacagtaa gaattgaagg agctaaaaat gctgttttgc ctattttagc tgctagtttg
    16441 ttagcagaag aaggtattac gacattagac aacgttccga ttctttctga tgtcttcact
    16501 atgaatcaag tgattcgtca cttaaacgta gatgtagatt ttgatgaaca aaagaatcaa
    16561 gtaacaatcg acgcttcaag acaattagaa atcgaagcgc cttatgagta tgttagtcaa
    16621 atgcgtgctt caatcgttgt gatgggtccg ctacttgcca gaaatggaca tgcaaaagta
    16681 gctatgcctg gtggatgtgc gattggtaaa cgaccaatcg atttgcattt aaaaggattt
    16741 caagctttag gagcaaaaat tattcaaaaa aacggctata tcgaagcaat tgcggatgaa
    16801 ttgatcggta acacgattta tttagatttc cctagtgtgg gtgctactca aaatatcatg
    16861 atggctgccg taaaagcaaa aggaacaacc atcattgaaa acgtagctcg cgaaccagaa
    16921 atcgttgatt tagcaaacat tttgaataaa atgggcgctc aagtctatgg agctggtact
    16981 gaaacaatgc gtattgaagg cgtggatcat ttacatgcag tcaaccactc aatcgtacaa
    17041 gatcgtatcg aagcaggtac ctttatggtt gctgctgcaa tgacacaagg caatgtgctg
    17101 attgcagatg ctatctctga acataactgc ccattgattt ctaaattgat cgaaatgggt
    17161 gcagaaatca ttgaagaaga aggcggagtc cgtgtcatcg gaccaaaaca tatcttacct
    17221 acagatgtaa aaacgatgcc tcatcctggt ttccctacag atatgcaagc tcaaatgaca
    17281 gctatccaat tagttgcaga aggtacaagt gtggtgactg aaacagtgtt tgaaaatcgt
    17341 ttccaacatt tagaagagat gcgccgtatg aacgctcatg tcaaaatcga cggaaatgta
    17401 gcaatcatgg atggaaacca tgaattgcaa ggtgcagaag tatatgcaac agacttgcgg
    17461 gcagcagcag cattagtttt agctggcttg aaagcaaatg gaatcacacg tgtgcgtaac
    17521 ttgaattatc tagaccgtgg atattataat ttccatatca aactacagca attaggtgca
    17581 gacgtcgaac gtgtagacat ggatcaaact tctgctgaaa aaacagcaca aacgattgct
    17641 taaggaagtg gctgaatgag taaccaacga tatatcttat taactttgat aaagatttta
    17701 gtggtgatct tattgcttat ccttcttttt gtggccggaa cgatgatcgg atatggtgtc
    17761 atcggtggcg gcaatccatt taaagtattc cagccaagtt tgtggattca tattcgagac
    17821 ttttttcatt gataaataag aatcacagtg gttctagtcg atatgaagtg agccatgaag
    17881 cagaacatga taaaagaaaa cagaagggaa cgttgacgtt agtcgcgatc ccttttttta
    17941 gtaaaagaaa agaaggaatc taattgatga agaagatatt gatttattta gttagaggat
    18001 atcagcgctt tatttcgcct gcatttccac ctagctgccg ttattaccca acctgttcca
    18061 attatatgat aaaagcaatt caaacccatg gtgcgctaaa agggacggta atggggactg
    18121 cacggatctt acgctgtcat ccttttgtca aaggcggtat cgattatgta ccattgacat
    18181 tccgattaac gcgaaataag aaagataaaa aagaaccaga ttataaaaag gttgtgaaaa
    18241 atacgggtag cactgagcga taaagaaaaa gaggaaattt attgtcaaag taacaactcc
    18301 gcaactactt atatggggga caaaaaatga tcgaagaact aaacaagaaa gatttaccat
    18361 ggtccttgtt attagatgca gatcctgata aagaaaaagt tcaagtatat gtttccagag
    18421 ggagtggctg gatatggaaa gaaaaagaaa aaacaatagg tgtactgatc tatgtggcaa
    18481 gggaaaaaga atacgaaatc gtcaatgtag cagtaacccc aacgtatcaa ggaaagggga
    18541 ttggcggaaa attacttgag acagcttttc agaagctttc acagctagct tcaaaccaaa
    18601 ctcggatcat tatacgtact ggcactacta gtagcgctgc gttacacctc tatcaaaaaa
    18661 tgggctatgt ggagattggt cgagtcaaag actattttat ccatcattaa tctgagccca
    18721 tttttgaaaa tggagatcaa ttaagggatc aagtgatttt agcaattcac ttacctaaaa
    18781 atagctttaa ctaataaact ttcaattcga gaaaatgctt tcaaatgcta aatagataca
    18841 ttacaatagc tttagcaaac aaaaaggaga atatttttat gggaatatta gtttttgatt
    18901 ttggtggatc ggctgtcaaa tatggttgtt gggatggcaa agagattaaa ggtaagggac
    18961 aatttgcaac gcctgaatct tgggaagaaa tgaaaacgca attatttcaa gtttataaaa
    19021 aaatactcgt ttcttttgaa gtcgaaggaa ttggaatcag ctctcctgga gtagtgaata
    19081 atgagaaaca ggtgattgag ggaatcagtg cgattcctta tattcatgga tttaatattt
    19141 tccgagattt ggaaactctg tttcaactcc cagtaacgat tgaaaatgac gccaactgtg
    19201 ccggaatggc tgaattttat gaaggtgctg cccgtgatta tcaagatgtt gcttttgttg
    19261 tggcaggaac tgggattggc ggggcgttgt tcacgaaagg acaaataaat aaaggggcac
    19321 atctttacgg tggagaattt gggttgatgt tcttagatgg cgataagaca ttcagtaaac
    19381 ttggtacagc ggtacagatg gcttggcgtt attgtgaacg caaaggtctc gataaaaatg
    19441 cttataccgg aaaagatgtc tttgaattag cagaaactgg agatgcaata gcaaaagaag
    19501 aagttgagag tttttatacg tatcttacaa agggattatt cagtattcaa ttttcgtttg
    19561 atccagaagt gatcgtatta ggcggcgggg tttctgcaaa agaagggctc attgatgaga
    19621 tcaaaagccg aatgaagaag ctgacagaac aattcgatct gcatgatttc gaaccgcaga
    19681 ttttattatg tgagtacaga aacgatgcta atcttgtagg tgcagcagca aactatctcg
    19741 ctgtacgtca aggagagaat taagagcttt agagaaggtg cagcttgcat aaaagaagag
    19801 aatgaaatga ataggagatt tttacctaag gaatgtttta tttgctgcag tatgataaaa
    19861 tatctgagag gtgaaaatga tgtcaaaaaa aaataaagat attgaagtgc gtatagaaga
    19921 agtgaaaaaa gatatcaaag gtaagaatta tgaagtcact cagttattta tagggaaaaa
    19981 aatgattggt gagatcttag cctatgggcc aaaagaatat gaaattttct ttggtgaaga
    20041 agattttgga aaagaaaaaa gtcttgaaaa tgcgatagaa acagtgattc gacactggaa
    20101 tctgcacgaa taaaaaaata tcaaaaaaac tttcaataaa gggttgcaaa aatctggaaa
    20161 attaggtatt ataactaagt caggttgata caaacctttc ggaggagtag cgaagtggct
    20221 aaacgcgacg gactgtaaat ccgttcctta gggttcagtg gttcgaatcc actctcctcc
    20281 atttcattgg ggtatagcca agcggtaagg caagggactt tgactccctc atgcgttggt
    20341 tcgaatccag ctaccccagt atac
//
LOCUS       DAFGFZ010000050        19962 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000050 DAFGFZ010000000
VERSION     DAFGFZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.050"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..352)
                     /locus_tag="LR726_001535"
     CDS             complement(<1..352)
                     /locus_tag="LR726_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBL6216633.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTT"
     gene            complement(565..951)
                     /locus_tag="LR726_001536"
     CDS             complement(565..951)
                     /locus_tag="LR726_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL6216634.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(1007..1630)
                     /locus_tag="LR726_001537"
     CDS             complement(1007..1630)
                     /locus_tag="LR726_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL6216635.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(1694..2362)
                     /locus_tag="LR726_001538"
     CDS             complement(1694..2362)
                     /locus_tag="LR726_001538"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL6216636.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(2374..2814)
                     /locus_tag="LR726_001539"
     CDS             complement(2374..2814)
                     /locus_tag="LR726_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216637.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(2873..3544)
                     /locus_tag="LR726_001540"
     CDS             complement(2873..3544)
                     /locus_tag="LR726_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL6216638.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(3729..4127)
                     /locus_tag="LR726_001541"
     CDS             complement(3729..4127)
                     /locus_tag="LR726_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL6216639.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(4124..4897)
                     /locus_tag="LR726_001542"
     CDS             complement(4124..4897)
                     /locus_tag="LR726_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL6216640.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(4894..5577)
                     /locus_tag="LR726_001543"
     CDS             complement(4894..5577)
                     /locus_tag="LR726_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL6216641.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(5744..6631)
                     /locus_tag="LR726_001544"
     CDS             complement(5744..6631)
                     /locus_tag="LR726_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6216642.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(6628..7497)
                     /locus_tag="LR726_001545"
     CDS             complement(6628..7497)
                     /locus_tag="LR726_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6216643.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(7634..8920)
                     /locus_tag="LR726_001546"
     CDS             complement(7634..8920)
                     /locus_tag="LR726_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6216644.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            9193..9771
                     /locus_tag="LR726_001547"
     CDS             9193..9771
                     /locus_tag="LR726_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL6216645.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            9771..10700
                     /locus_tag="LR726_001548"
     CDS             9771..10700
                     /locus_tag="LR726_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6216646.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            10697..12433
                     /locus_tag="LR726_001549"
     CDS             10697..12433
                     /locus_tag="LR726_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL6216647.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(12496..13332)
                     /gene="pheA"
                     /locus_tag="LR726_001550"
     CDS             complement(12496..13332)
                     /gene="pheA"
                     /locus_tag="LR726_001550"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL6216648.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(13329..13838)
                     /locus_tag="LR726_001551"
     CDS             complement(13329..13838)
                     /locus_tag="LR726_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL6216649.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(13831..15126)
                     /gene="aroA"
                     /locus_tag="LR726_001552"
     CDS             complement(13831..15126)
                     /gene="aroA"
                     /locus_tag="LR726_001552"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL6216650.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(15139..16230)
                     /locus_tag="LR726_001553"
     CDS             complement(15139..16230)
                     /locus_tag="LR726_001553"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL6216651.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(16247..17413)
                     /gene="aroC"
                     /locus_tag="LR726_001554"
     CDS             complement(16247..17413)
                     /gene="aroC"
                     /locus_tag="LR726_001554"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL6216652.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(17415..18479)
                     /gene="aroB"
                     /locus_tag="LR726_001555"
     CDS             complement(17415..18479)
                     /gene="aroB"
                     /locus_tag="LR726_001555"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL6216653.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(18512..19534)
                     /gene="aroF"
                     /locus_tag="LR726_001556"
     CDS             complement(18512..19534)
                     /gene="aroF"
                     /locus_tag="LR726_001556"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL6216654.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         5737 a         4361 c         3484 g         6380 t
ORIGIN      
        1 ctgttgttcc aagtaaagaa gtcaatacag tcgcactaat tttccctagc ttttcagaaa
       61 ctttcatttt tgtgaatgca ccaatgatcg aaataaacac taaaggcata actagcattt
      121 gcaagaatga tacatatcct tgacctacaa tcccgatcca ttccattgat tgagtaacga
      181 ctttgtcgtc tacaccaaag atcaattgaa gcagaccgcc aaagacgata ccaactccta
      241 atgctgtaaa gacgcgtgtt gaaaattttg tatggcgctt ttgcatacga tagaacacat
      301 ataataatgc cgcaaaaact agaaccacta aaactgtgat gagtgttgtc atttttctcc
      361 tcctcatatt attaatgaca agtccatcga aggaagtaaa ggttcccgag aatgattctt
      421 caaccatcct cctgaaaaaa tttttttcag tgacttttat attttagacg atttttatcg
      481 aaatgacatt ctttttgctt acaattagta ataaaaaaac atacctgtga tagctttaat
      541 ctatcgcagg tatgttttta ctgtctattc ttgtttttct gattgaaaat tcaattttac
      601 cccaggctga tctaaggcga tctctgtcac ctcataagac attcggcgta cgatatcgaa
      661 taacggtgct actagctcgt ccacttcttc ttgtgtgaca tcattttgac cttcaatttt
      721 ccgattgagg atatgattga tctgactcac acttccagat aaaacatatg tatcaaacac
      781 taaacggaac tctaagcgtg ctcctacgat gctatgttct ttcggatagt tttcgatcgt
      841 ttcttttaaa ggcgagaagc ctactttcaa atctgtttct acttcttggt tcggcaatct
      901 catatcataa tgaaatgctt ctacaacttc tttttgtctt ctgatttcca tataatttta
      961 tcactccttt atgaaaatta taacatatct cctttgaaag gggatactac tttttaatga
     1021 tgctctgcaa ggtaaggatt tcattcagat ttcctaccac ataatctgcc gggatctcac
     1081 ccagaaaatg atcgatgtcg taaagacaag tagcgactcc tgcatttttc cctgcttcga
     1141 tatctaaacg acgatcgccg atcatcatcg tgtctgttct ttctaaatga aaagtatcga
     1201 tcaaataatt taaagatgct ggatcaggtt tacgaggaaa gtcctgatcg atccctacta
     1261 cttcctcaat cagatgtgcc aatcgatgtt cttttaacag gccccatgtt gattctgtca
     1321 aacggtgggt caggatgaaa tgacgaccgc cgttgtcttt tagcatttcc agtacttgtt
     1381 tcgtttctgg aaatggacgg gaaacttccg tactttcttt ttcatatcgg tgaaataatt
     1441 cattaaactc tctggcattc aagtttgatt cattgatcaa ataactagta gaatactttt
     1501 tcattttgaa gtagatttct tttttgtcca tactgatacc aaaatctttt agtgcttgcc
     1561 atgcaccatc taccattgcg ggatacgtat caaacaatgt accatcaaag tcccatatat
     1621 aatttttcat gttccttttg ttccattcct ttgcttcttt tttctatctc tcacttttag
     1681 ataaaggtgt tctttaaagt atttttttgt ccagaattgt caaatcatct gctaatgtat
     1741 actggattgg ttgtgcattt gggatatcga tcgtatccat ttgatcttca ggaacatctt
     1801 ctaaatattt tgtcagtgct cggatgctat taccatgacc cacaactagt aaatcttgcc
     1861 gatcttttag ttttggtgct agatgatctt gccataaggg aatgatccga ttcactgtca
     1921 tttccagact ttctccgtat gggatcaaat gcggatccaa gtgatcatat ctgcggtcaa
     1981 atctattttc tgcggttaac ggcggaactt ccgtatatcc tctgcgccat gtttttactt
     2041 gatcttttcc gtatatttct ttcatcttat ctttgttttt acccacaaga tccccataat
     2101 gccgttcatt tagacgccat gtttttatta taggaagcca taactgaccc atttcttcta
     2161 gtgcgacttg tccggtaaga atcgcacgtt tcaaaacaga ggtataaacg gcatcgaatt
     2221 gaagtccggc ttcaagcatt ttttgaccgg caactgctgc ttgttctttt cctttttctg
     2281 ttaaggcgac atctagccat cctgtccagt aattttcaaa atttgcttcg ctttcgccat
     2341 gacgtaataa tactaatttc attcttttct cccttaaaca ttcgtttcga attttatacc
     2401 acatgttttg catgtcacac gtattcgtcc ttttccttta ggtgcacgct gcttttgatg
     2461 acaattcggg cactcaaaaa aagtataact ttttcgatcc attttgactt gctttttgtt
     2521 ttttatctga tgatattttt ctttgactct atcaagatat ttcaaataac gttgattctc
     2581 atttagtctg gggtagattt tttttgatag gaaacgaaat atcatccatc cgaaaaagac
     2641 gatgaacaac aattgtccga ttctataggg caaccaacga cttagcaaac taaagacaag
     2701 cgcagcgatc aatagaccgc gattcaactg atcaaatttc gcatatcttc cctgcataag
     2761 tttgtgatag ttctgctgaa atcgtattag cctttctaac catgtttgca tcatattatc
     2821 tcctccatca tctaatattt attatgacaa aaaaatccaa aaagaaaaag ccttaattgg
     2881 ccttttccat tgcaacttgg tgaaggacat tccgatttct ggtaaagtca atcacaatca
     2941 ctttcttttt atcttctctg ttgactactc gaaatgtgtc cgtcaatgga agaaacagct
     3001 tcttagatgg tagctgttgc atacgtataa aacgatggat catcgtaatc aattgaggaa
     3061 attgaataga cgtgtgaagc agacctggat attttttcaa cagctgtgtc gttttgatca
     3121 gattccgttt tttactgatc acttttaaaa agctttttag aatcttttgc tgacgtctaa
     3181 aaatagaaaa agaattttcg tcatctattt gataacgcat ttgtttttgc agttctgctg
     3241 cagtcagatg gatcttccca tcgtttgtat cgatcattaa accttctgga aacagttcaa
     3301 ccactgctcg atcccaagat tcaagagaaa gctcgatata atcttttact ggtaattcca
     3361 attctacctt aaaaaagtgc aaaacagccg acagcccttc tgcttgatag atttctccta
     3421 atgttagccc ctctagatgg aactctggtg aaaaatgcag caatgtttta ttttcctgca
     3481 attgaacata tagaaatgta gatcgctcaa tcagagtatc ttttaaaaca actaaaaaat
     3541 tcatttttct cccccttttt tcatcatttt taaaatagca tattttgaaa agaaagtctg
     3601 atgcttagag tagaatttca taaaaattta ctatttcata agtattcacg agaaaaatac
     3661 aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt tgtgtttatt tcctcttcaa
     3721 acgcttgtct atgattcaga cttttctcta tcgttatttt catctaaaag ttcttgcatt
     3781 ccgataattt ctcgatattg atgaatctta gtgataccaa tgactttgtc tacatcaata
     3841 ctaaaacaaa taccattacc tggaatatcg atgttgatgc cttgaccaat cgcatccatt
     3901 actttattcg tcaaactatc gggtactaat gtcaacacaa tatctttttc tggcgaaatg
     3961 tccaaaccta aaaatttccc tgaatcatgt acaccagaac ctcttccatg taagatcgtt
     4021 ccgcctgctg cacctgcttc ttttgaaaag tcgataacat cttcgcccat tcctccgtca
     4081 acgatcgtca caatcatgtg aagattcaat gctacaagat ctttcatttg ctttccccca
     4141 atttttgcaa tctttctttt tcgattctgt ctttttcatc ttttcgttta taaatatatc
     4201 ctagtactaa agtggataat attggtgtca atgcaatcat tgcaaccaca ccaaacccgt
     4261 caatgatcgg atctcttcct tcaatttgtg cagcgattgc cacgctcatt gataaaataa
     4321 atgtcgaaca catcgggccc gttgctactc caccgttatc aaaagccatt gctaaaaaga
     4381 tcttgtccac ttttcgtcct aaaataaatg ctagcccgta tcctgggatc aagaaataat
     4441 aaagagagaa tccgacgagc aaacgccaca tagacaatcc tactgctgcg ccgatcccaa
     4501 ttgaaattac catcagcatg attttcgatc ggattctccc ctcgctcaat tcttcaacct
     4561 gtttgatcat tacatgaatc gccggctcag caaaccctac taagaacccc attaaaaagc
     4621 caaggggaac cagtatataa ttgtaaggca atcccgcaat agcactgcct aaatgctgtc
     4681 ctaccggtac ataagccata ttgactccgt gcaggaaaag gatcaaccca atagtcgtta
     4741 tcaagaaacc tttcataatg tcaaagaaac gctttttgcg catacgaaaa ctaatgatat
     4801 tcattaaaac aaataggatt acgataggtg ccactgccaa aatgacttcc atggtaacga
     4861 tttcgattcc atctatcact tgctgccaga aactcattgg aagatcaccc ccaaaatcat
     4921 cacggcaaga atcggcccta acgaagcaat accgaccatt ccaaaactgt cattttcatt
     4981 tttcttttgt ttgatcatac tagtcatccc tccggcaagg gccaagataa atggaacagt
     5041 tactggacca gtagtcactc cccccgaatc aaaagcaatc ggcataaatt cggaactggt
     5101 gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc atcaattgat aataggatag
     5161 cttgaatacg acccgaagca gcgcaaaggc tagaaatact ccagtgccga cgctgacgat
     5221 cccaatcagc aacactctgc caatctctcc gtcagatata tcatatactt gttgtcccaa
     5281 aacttgcacg gaaggttcag caatcgtgat cacgatcccg atagcaaatc ccaaactgac
     5341 caaaacggtc aaacttttct ttttgatcat atatttccct actagatctc cgacttccat
     5401 catagaataa tctgctccaa acaaaaagag cgtcattccg atcatcatga ttgccgcacc
     5461 aaccagaaat gaagcgaggt cttctccttc taacggtgca aaaacaaacg ttaaaatgac
     5521 aatcaggaca gtcatcggca atatagcagc aatcacttct ttaaaatttt ttagcatgta
     5581 tcctcctcct ttactcttta agtttacctt aaaatgaaag agttttcgag cacaaactta
     5641 aaacgttatc atttaatttt tctcaacata aaataacaca cgtcccaaaa gtatcttttt
     5701 acttggaaaa atgactttga aggaacgcgc ttattgtgtt tttttatttt acagagccga
     5761 tcattccttg aacgaattgc ttttgcaaaa caaaaaatac gatggcagtt ggtaaagtag
     5821 cgatgactaa tccagccatg atcattccat agtctggtgt ataggaagca cccatagcag
     5881 ataaaacaag cggaaccgtc cgtttatcag gagattgcaa agctactaag ggccataaat
     5941 aattgttcca gctactcatg aatgtgatga ttgcagccgc ggcataggta tttttagccg
     6001 tcggcatata cactctaaaa aaaatactta gttcttttag accgtctaat cgagcggctt
     6061 ctactagatc ttttggaaaa gatttaacat tttgtcgaaa gaagaagatc aaaaaagcag
     6121 tgctaacaga tggcaggatg acaaccaaga aactgttgat tcccaaaacc gttccgttca
     6181 attgtgaaaa cattcgatat aaagggatca tcaatgccgc aaaaggaacc atcatcgata
     6241 gcagtagaat attgaaaatt aggtcttttc gtttactttg ataaatctca aatccatatc
     6301 ctgccattga cgaaatcagc aatgcaaaca cagtggtcac caaagcgatc atcgctgaat
     6361 tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa attttgaaaa ttgatgatca
     6421 agttactgcc aatctttatt tttcccgatg tgatatccac gggtgtattc gtcattccca
     6481 aaaccatcca gacaaaaggg aaaatggaaa taaatgatag gatgcttaat gctgtatact
     6541 ttaatactga cccaattgtc gaacgaagtt ctaatgcttg ttttttgacg ttggggcgtt
     6601 cgattgtttc tttttctacc atatttttca tcgactttct cctcctactt tcgtttggat
     6661 tatcgacaat atcacaataa aggaaacaat aacaaaggaa actgctgccg catatccaaa
     6721 attaggcgtg aatttataac tcaaattata gatgtattga gatagggtgg tcgttgcatt
     6781 tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa agctgcaatg tcccgatcgt
     6841 cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt gggatcgtca ctttgataaa
     6901 ctgctgccat ttagaagcac cgtcaataga tgctgcttca taaatctccg aatcaatatt
     6961 ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc caacgccaag tgatcgaaag
     7021 aatgatcaga actctcgccc aaattggatg agtcaaccag ccaatcggtg tgtcaatcat
     7081 accaatgccc aacaacaagt tgtttacaag gccagattga gaaaatagac ttttcatgat
     7141 cagagaataa ctgaccatcg aagtgataca tggaagaaag atagcggttc gaaacaaccc
     7201 tttatatttc aactttttat catttaaaag gttggaaata aaaagtgcta gaaacaacat
     7261 gatcggtact tgtacaagta aatacaggaa tgtattaaaa aaagcttttt taaaagttgc
     7321 atctgtcaac atccgctgat aattacctaa cccgttaaat gtcatattga cagggggacc
     7381 actttgaaaa gacgtgatca aagccaaaac catcggaata aaaaccatga ttgatatcaa
     7441 aatgactggc aagattaaaa aagcatgccc gtttttgaat aaatctttct gcttcattct
     7501 tttccctcca attctatcat tgattatcta catgaaaaga caagaaagac aatatctgta
     7561 aggcagacgt gatctttttt gtcttttcgt ttgtctgatg aaaaagcgaa gaaattcact
     7621 tttttaccac cgatcaatta gccaattgtg cttctacttg tttttgtgta tctgctagta
     7681 ccttgtctgt ctcttctcca ttgatgattc gatgaagagc ttcagctacg actgcttcaa
     7741 ttgcataggt ctcatgtccg taattcactt ctggtatttc agatgtccat gttgaaaaat
     7801 cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc ttgataagct tcttgttcat
     7861 tcgcgctaag catcgtagat actagtccga tttctttcgc aagagtgccc atcagttctt
     7921 cattcgaagc aaacgttgct tctaggaaat ctttcgctga atcttctcca gctactcctt
     7981 tgatcacata ccagccagcg ccgccaaggt tagaagcttg cgctttttgc agattttctg
     8041 gaagtgcagg tattggtgca atcttccatt ttccagattg gttttctgct ccttggatcg
     8101 ttgaagaata ccaagcgcca gttgggctag aagctactgc tcctgattgt atcgcagtta
     8161 ctccagcatt ccagtcagag gtttgttcga ctagatcttc tttcaaaaga gtggcaaaca
     8221 attcaagacc atactttaaa gatgcattgt cttggatcgt gactgtctcc ccatcttcag
     8281 atgtatacca ttctcctgcc tgttgcataa tcatccttac tcgaccaagg tcagaaggat
     8341 tgacttcagc gattttcttt cccgtctttt ctttgacatc tcgcgctact tgaatatact
     8401 cgtcccactt cagattctcc atatcctctt ctgtataacc tgcttctgcc atcagatccg
     8461 tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac accgtatatt ttatctccta
     8521 ctttgtttac cgcaaattta tacggtgcaa aatgatcttc ctttacaata gcagacaaat
     8581 tagcgaatgc atctggataa gaagtcaaat agccttgtat cctataatct tcaatcaaga
     8641 cgatattggg caatccttct gtattcccgc tcgctaaagc ggtgttcagt ttctgaacga
     8701 tatcatcctg agacatcgta acaacttcta tctctgtttc ttcgttctca taaacttttt
     8761 ttgcttcatt cactgctttg atattgaatg tctcatccca cgcccagaca gtgacttttt
     8821 cttctttgtc tgctgtacta ctagcacttc cgcatcctgt taagaaacct gatgctgccg
     8881 ctaaacaaac gactccagca attaactttc tgatgttcat ttttatccgc tccttttcca
     8941 tccattagtt gtttacgttt gctacattca tcataccaaa atttgaaaac ggtttcttta
     9001 ttattcagat acttcaaaaa tttctcctaa aataataaaa aaatacaaat caactatgac
     9061 aaaaggaata atcttagcat ctagaccaaa gatcgaaatg gtgaggtttc tttaaaataa
     9121 aaatatcctt gcattttctc ctagaaataa gtatttttaa attacaagac tattcttaag
     9181 gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg atgaacagac gattttagat
     9241 ggaatgaaac agttgatcaa ttggtctgag cacgggtttc aattaatagg gactttttcc
     9301 agtccgctga aagcgcttga tttttctttg gtacatgcaa tcgatatcgt ggtcacagat
     9361 gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc tattaaaaca gcaacaacct
     9421 gaaacacaga tccttatcct ctctagctat gatgaatttg atatggttaa agattctttt
     9481 aaagaaggcg tcgctgatta tcttttaaag cctcgattga gtcctgattt ttttctaaat
     9541 tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca acaaaagccc aatcaccaat
     9601 cgttatgagc agatctgcga gaatctttca cagcagattg ctggacaaac tcgtccattg
     9661 cttgaccctc ttctttttga acacaccttg tttttctgtt atataccgaa aaacggccat
     9721 tcagttcaaa acaaggctct aaacaaaaac agtcgatcac ccattcattg atggatagcg
     9781 acgattcgtt tactatttat cctgtttcta ccttttcgga agaatccggc tatgtaatca
     9841 atactgattc caaagataca cttaggaagt ttgttcaaaa gtttcgtcaa gaagtttttc
     9901 caggtgattt ttttgttgtt tctgattctt ttgcaatcca agatatcaat gaaatattcc
     9961 aagagatcag aaattgcacc aaaggacaac gtttctatca taagggaaag aaccttgctt
    10021 atcagcatga acttcttccg ttattaacca accacgagca gcaagcaagt agttatttaa
    10081 caaaaatcgt tcataaagac tacgctttag ctgtcaaaga acttgagaat ttcttcacga
    10141 cgatattgga acagcctatt gatccagctt atttgaaaca tgaggcaatc aatcgtttct
    10201 atgcattact caacgctttg gctgacgagc atgttgggaa tgaagaactt tctttgctca
    10261 agatcgccct tccttcttta ttagccgatg ctgagtattt cgaagatttt tctattcttg
    10321 tcaaagaaac acttgagaaa ctcacccact tttctgcaaa tgcaaaaaaa gacaatttcg
    10381 atatcttgac tttgatctac gagttcgtcc aggaaaatta tgagaaagaa atcaatttac
    10441 aacttttatc cgaaaaatat catttttcat atagctatct ctcagccatc tttactgaaa
    10501 aatttggtat caacttttca aaatatctaa aaaaagtccg tatcagtaaa gccaagctct
    10561 ttttgacgga aacacggctt actttgacgg aaatctgtga gaaagtcggc tataccgaac
    10621 tgggttattt ctccagagtt tttaaagaag agacaggaat gacaccttct caataccgta
    10681 aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat gaactctttg ccaaacttct
    10741 gttcattttg ttcatcggtc tttttctgca agccatcgtc atttctttgt ttatttatca
    10801 tcgatcaagg gatgcctata tccagctgtt caatcaatcg aatgatgtgg tactaaaaaa
    10861 gattcaatct gaatttgaaa gtttgaatga tacaatcgag aatacattag ccgcttttga
    10921 ttctaatcca gctgtcaaaa gctatttttc caatgatcca gcccaacata tggaacagct
    10981 tcagcaacta agaacaatcc aaaaaatgaa tgactctctt tcaaaaatcc atccaatgat
    11041 cgattacgat gtattgatct ttggagaaaa tggaagaact tttgtcggaa acgacatgct
    11101 cactgccgta tctgccgatt ctttttttca atctgcgatt gctcagagag tgaatgaacg
    11161 tgctgctgat acacaaatgc tttttgcaca ccacggattg actcttcgcg ataagaaagc
    11221 acccagcgtc ttctttgtca aaaaactgaa aaatacattg aatcacgttt acggatatgc
    11281 tgttttatcg atctcatcca agcaactagc gaatcttttt caatctgtag taaatcctga
    11341 gatatcgaag atctccttca tgaacgaaga acagacgatc atcgcatcaa atgaagaaaa
    11401 tacaattggg agccgatcaa tgattttcaa aaaactaaca gtaggtgaaa caacgatttc
    11461 aaatggacag cagttgaccc gtcttcctct gtaccgccag gattgtacat tactcagtca
    11521 agtcgatctt cattctttat ctaaccaaat gggcgtgatt tttccgatta ttttgtataa
    11581 tatcgtgaca cttgcttttg ctggctcatt ggtatttatg tatttggatc gacacacaaa
    11641 atcgatctac cgcttgattg attcgctaaa acggctagag gaagatggct taaaagctac
    11701 tgttcctgta caaggaagcc atgaagtcca attgctaggg aatacgatca atcaattgct
    11761 aagtaatgct cagcatcatt atgaacagac gatccaagac gaaaagaaga aacgttcgct
    11821 tgaaatccaa actatgcaag cacagataca gccccatttt atttataata cgctgactag
    11881 cttgaaattt ttgatttggc agaaaaaaac agatgaagca gttcaaggaa tcgacagttt
    11941 cattacttta ctaagaagta cgattgggaa aaaagaagaa gtgattccag tcaaggaaga
    12001 aatcaaaagc gtacaaagtt acatcgatct tttgagtatg cgatatggcg aaagaatctc
    12061 ggctaaaata atgattcctg atgaactgca gtcattatct atgccgaata tgatccttca
    12121 accaattatc gaaaacgcct atcttcatgc ttttcacaat aaaacgcaag gattcatcac
    12181 ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa gtgattgata acggcgatgg
    12241 cttcgatact gaaaatacca aacaaaaaaa tgatcatttt tcaggaatcg gcatcagcca
    12301 tgtagatgag cggatacgcc taatgtatgg cgatgcatat ggcttaacga tccaatcagt
    12361 gattgggaca ggaacgactg taacgatttg tctaccgctt cttcctaaaa atcaatcaga
    12421 atatcaaaaa taaagaatag ccaagcgctg atttttttat tttaagttaa ataaatcaat
    12481 cagtattctt gactttcatc tacgcgtctc ttctactgtc agcactggat aacagcccaa
    12541 tatttgactt tctcctccga gcatcttgat ttcttggagc gcattctcta ctaatgtcat
    12601 tggacggtcc aacaacaaat cgatcacgaa aaaatattct cccaagctag ttttcaatgg
    12661 tcgggattca atcttactta agttgatttc ccgccagcca aaagctgcca gcattttatg
    12721 aagcatccct ggccgattag caggtagtgt caagatgaca ctcattttta ctggagctga
    12781 ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa cgtgtctggt tcaactcatt
    12841 atcttggata ttctcagcta agatctccaa tccaacatgc tgtgctgttt ctaatgaagc
    12901 aatcgctgct gcgtcttctt tcggatgttc tgctacatac attgctgcag cagttgttga
    12961 ttctgttgct actaaaggaa cattaggata atgcgtttct aaaaactgtt gtgattgtgc
    13021 tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt gctggattgc ctaaaagctg
    13081 ctgcttgata ggaagaacga tttcactttt aacttcgact tctggatgtt tggaaagaag
    13141 atcgattgtg tgatgaacag ctccttccaa tgaattctct acaggaacga cagccagatc
    13201 gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg gaagcatacg cagtcaattg
    13261 ttctgtagga aatagctgac aagccgcctg ataagtgaat gaactttctg gccctagata
    13321 gctgacgttc atatgctttt gatcctttct ataatggtat cgatgatctt ttctggtgtt
    13381 tgattggtcg tgttgattcg atgtgtcgca ctttcttcat accaagaaag tcgttgtgcc
    13441 aataatgctt ttatttcttc tcttgttttt ccaagagcta aaggtcgaat agacgtttca
    13501 tcttgttgga tacgatcaac tagacagtcc gcttctgcct ctaagtaaag aacaatcggc
    13561 tgtgttttta aaaaacgccg attttctgca ctttggacaa tccctccacc agtcgcgata
    13621 attttgtctt catttgacag ctttcttaaa atatctgact ccacttttcg aaagcttttt
    13681 tcaccgtaat agtgaaaata ttcggcaatc gacaattgga tatgttcctc aatctttgta
    13741 tctaaatcta catactcttt ttgtaaacat tgagctaacc ctttaccgat tgttgttttt
    13801 ccagcgccca tgaaaccaat cagcaaaatg ctagccatga gcttctcctc ctgacacaag
    13861 ttctgctaaa tcgtcaaaaa atgccggata agagattgaa acagcttcag ctccttctag
    13921 ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc atcataccga ttcgatgatc
    13981 gcctctgctg gacacttttg cgccatgcag aggtgtcggc ccatggatga tcagaccgtc
    14041 tgctgtcgct tttatctttg ctcccatttt ctgtaattct tctgccaccg catcaattct
    14101 gttggtttct tttactttta attcttctgc gtctttgatg atcgtgattc cttctgcttg
    14161 agtagccact aaagctagaa tgggcaattc atcaatcaga cgcggaatga tttctccacc
    14221 aatcgttgct ttcttcaaat gactcgtctt gacacttaaa tcagccgatt ggttctgttc
    14281 attgtgattc gtctgggtaa tcgctgcgcc catctcctcc aacacatcta agataccagt
    14341 tcttgttgga ttgaccccga cattttttaa caacagttgg ctttccggaa caagaagtcc
    14401 tgctgctaga aagaaagcag ctgatgaaat atcacctgga acagttatct gttgaccagt
    14461 taatttttgc ggtccggtca ctatgatcgt tttatcttcc actgtgatcc ttccgccaaa
    14521 ttgacgaatc atttcttccg tatggttacg ggaacgttct ttttcaacga ttacggatgt
    14581 gccttctgct tgtaatgccg caaacagcaa agcagatttg acttgcgcac tggctacagg
    14641 catattgtat cggataggtg ataaagagcg ggttcctgtc acagtgattg gtagatactg
    14701 gtcattttct tctccttgca agtcagcgcc catctcgcgt aaaggttcca tcactcgccc
    14761 cattggacgt ttagacaacg aagcatcccc gaacagagta gtcgtaaaag gctgtccagc
    14821 caaaataccc atcaataaac gtatggtcgt accggagttt cccatgtcaa ttgattcggt
    14881 tggttggaca aatgagtggc taccgcgacc atgaatcttg atcacctctt cctcttcttc
    14941 gatctcgaca cctaattgtt ggaaggcatg taaagtactc agacaatctt ctgcgcgcaa
    15001 aaaattttgt atagtcgtcg ttccttcagc aattgctcca aacatgatgc tgcgatggga
    15061 aattgattta tctgctggta tcctaacagt ccctcttaat ccatgtattt gttgtaataa
    15121 ttgcatttat ccagcctcct atttgatcca gcaatgataa ctggtattag acataatgat
    15181 tctttttgct tgtaacaaat cctgttggtt tttaaaactg atttgcagta ttcctaaaat
    15241 atcttccctg gtctcttgga tcttcaaatt aatcaaagaa actcccgctt ttccgatcag
    15301 tccagttact tctgcaataa cgccaggttc atctggcaca tcgataaata aatcaaaaaa
    15361 ggcaggaatc gcgccttgct tatggacagg caactggtct cgactttctt ttgcccgatt
    15421 gaaaaaagaa aagatttccg attggttctc agtcatgatc cattctgcta tttgtgtcat
    15481 ttcttgttgc caatctgcca atcgttccaa taatatctgc ttattgctca acaaaatatc
    15541 tgtccacatg acaggatctg aggaagcgat cctagtgata tctcgaaagc ctcctgctgc
    15601 cagttgtttg gctcgcggat actcttgatt gaattgttct gcctgattga ctaagccagc
    15661 tgccagaata tgtggaaaat gacttagcat tcctgttata cggtcatgtt ctcttgctgt
    15721 aagagttaca tattttgctt tggttccgca aaatagttct tgcagttttt caacatcttt
    15781 tttcgtcttt tccaaaggtg taaagatgta ataggcattt tcaaatagtt tagcatctgc
    15841 tgcctgtata cctgatttat gagaaccagc cattggatgt ccaccgacaa accgaagatt
    15901 tttctgctca gcggatgtca cgacttgctg cttcgtactg ctgacatccg taatgatgac
    15961 gtgatcagac aagttgattc tttcaaggac atcaagataa gctaaggttg tacggacggg
    16021 aacggctaat aagataacgt ctgcttgttc tgcacctgtt tcaaaatcac ttgcaacgta
    16081 atcaacgatc tgctgttcta ctgcttgctc acgggttttt gactgactat cccaaccaac
    16141 tactatcagt tctggattgg tctgacgaat acaaagtgcc atagaagcac caatcagtcc
    16201 aagccccacg ataaaaacat gttgtgccat cttatttgcc ccctttttag taatgtttca
    16261 agtagtcacg gtactccttc actgcagctt tcaactcaac aagcgaatca cttgggaatt
    16321 ttgacaatag ctctgtcgct accacagtgg ctacaacatt ttccgcaacg acgctagctg
    16381 caggaactgc tgtactatcc gaacgttcta cacttgcttt gtaaggttct tttgtatcga
    16441 tatcaacact ttgaagtggc ttgtacaatg taggaatggg tttcatgacc ccgcgaatga
    16501 caagtggttc gccattggtc attccgcctt cgaagccgcc tagattattg gtacgtcgga
    16561 aatagcctct ttcttcttcc cataggatct catccatcac ttctgagcct ggtaatgaac
    16621 cagaagtgaa tccgacccca aattctacgc ctttaaaggc attgatacta accacagctt
    16681 gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta gcttcctaat ccagctggaa
    16741 cgcctccaac gagcacttct acaattcctc cgatcgtatc tccatttttc tttgtctgat
    16801 cgatcaaatc ttttattctt tgctcaactg ctggatcaac aactcgtact tctgacgctt
    16861 cagaaatttc tttgatttct tgaatcgaga tatttccagg tatttctgct tggattccac
    16921 caagacaaat cacgtgtcct gctacgatga tgtcaagttc ttccagaatt ttcttggcaa
    16981 ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga ggaccgttca agcacattcc
    17041 gaagatctcg atgatggtat ttcataccgc ctaccaaatc cgcgtgtcct ggacgcggac
    17101 gtgcaactcg ccgcagtgat tggtcttttt caggcacttc tgctacagac atgacagttg
    17161 tccaattttt ccaatcttta ttttctacca ctaaagtgac aggtgaacca attgttttgc
    17221 cgtgacggat gccagacgtg atccgaacac gatctttttc gatcttcatc cttcctcctc
    17281 tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat ttcttcttct gacagaggta
    17341 atcctgctgg tagtccttca ataatggctg ttaattctgg tccatgggat tctcccgcag
    17401 tcatataacg catcttacag tcctcctaag taatctttga atgtctctgt gggaacagga
    17461 acgatttttg cttgcccaat cttttctagc aagatgatat tcaattggct tcctcttgct
    17521 ttcttatcat gggttatcgc gtggaataat tcatctggat gacttagatc gtacgtaatc
    17581 ggcagatgga atttttgaag tatcctttct aaagcagctg tcgatccagt tggagacaag
    17641 ccgatttttt ctgcatgaga agtgatggct accattccaa tagctactcc ttctccatga
    17701 ctgactaccc catatcctgc tgtattttca attgcatggc caattgtatg accaaaattt
    17761 aaaataagtc gatttccttg gtcaaagaca tcttcttcca ctacttttcg tttgatttcc
    17821 aacgtagcag taatgattgg aacagcatgt tccagaagat ctttttcgtc tttcagctcg
    17881 ttcagctgtt tccaaagcgt tgcatctgct atagctgctg atttgatgat ttcagcgatt
    17941 ccttctcgta aacgacgtac cggcaaagtt tttagaacct caggatcaat caatacacca
    18001 gctggctggc taaatgttcc gacaaggttt ttcgctttgt ttgtattgac tgctgtcttc
    18061 ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg gaatttgaac gaaatggatc
    18121 ccacgcatga acgtcgaggc cacgaatgct gctagatcac ccaccattcc tccaccaagt
    18181 gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta aaaaatcgta taactcagct
    18241 gcttgttcta aagatttact ggtctcaccc gccggtacga ttgaaaggac tggttcaaaa
    18301 ttagcttgtc tcaattggaa catgattttt tctgcatata gtggagcaac gtttgtatca
    18361 gtgatcacag ctactttttg aggtctccaa atcgtttgaa gccatgatcc agcctgttct
    18421 agtgcgcctt tttcaatatg aatatcgtat ttttggttat caacatgtac agtaagcatt
    18481 cgtttcactt ctttctttta aaaagctgga attaatcgag tgtctgaatc ttttccattg
    18541 cttctttcat gatcgcaact tctttcatca tgcgtgagta agtcttttca ttcaacgatt
    18601 ggggaccgtc tgaccaagca tttgcaggat caggatggat ctctacgatc atgccatctg
    18661 ctccgcttgc cacacccgca cgagccattg gtggtacaag ttcccaaatt cctgtaccat
    18721 gacttggatc aacaatcaca gggaaatgag ttaatttttt catcaatggg acagcactta
    18781 aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat cccacgttcg ataaaaatga
    18841 caggagattt gtcttcgact gcaatgtatt ctgcagcatt aagccattca ttgatcgtac
    18901 cggaaattcc acgtttcaat ccaacaggtt tacctgtttt accgacagca gatagcagtt
    18961 taaaattttg catattacgt gcgccaatct gtaggatatc gctgtactca gccaccatat
    19021 cgatatgccc ttcatccatc acttcagtaa tcactttcat tccaaattca tcagccgctt
    19081 gacggatgta tttcaatcct tcttcttcta aaccttggaa agcatatgga gaagtacgtg
    19141 gtttgaaggc accacctcgc agaatttttg cgcccccggc tttggccatt cgcgcacatt
    19201 ctctgatttg gtctaatcct tcgatagaac aaggaccagc catcgtcacg aaactgccgt
    19261 caccaatttt tactccgtct acatccacga ctgtattgcc aggatggaat tctcgagaag
    19321 tcaatttgta tgtgtttaaa atacgcacag cattttctac tccttcatag cgtgtaaatg
    19381 ctccttcttg taaacttcgc gtatcacctt ttagtccaat cactactcgt tctgttcctt
    19441 gatcgattga gactttgaat ccttcttttt cgatatgatc gacgactttt tttatttgtt
    19501 cttccgttgc ttcttttttc ataattacta tcattgtttt tcccgctttc ttttgtttac
    19561 ttatctatct tggctgattt tctctacttt ttcaaggtgg ttttacatta attcctacta
    19621 aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt cctacttatg acccagataa
    19681 tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca gtgaacatct tcaacgagcc
    19741 ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat gaatttctct acgtggctgt
    19801 aagtcgtgtt taaaaatctt tagccatcat agatacaaac ctggtttgtt tgtacccacg
    19861 aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat acgtattcag ttttgtaagt
    19921 tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag aa
//
LOCUS       DAFGFZ010000051        19937 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000051 DAFGFZ010000000
VERSION     DAFGFZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.051"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            182..1114
                     /locus_tag="LR726_001557"
     CDS             182..1114
                     /locus_tag="LR726_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216655.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1335..3098
                     /locus_tag="LR726_001558"
     CDS             1335..3098
                     /locus_tag="LR726_001558"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL6216656.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3182..3640
                     /locus_tag="LR726_001559"
     CDS             3182..3640
                     /locus_tag="LR726_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL6216657.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3749..4432
                     /locus_tag="LR726_001560"
     CDS             3749..4432
                     /locus_tag="LR726_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL6216658.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4579..5193
                     /locus_tag="LR726_001561"
     CDS             4579..5193
                     /locus_tag="LR726_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL6216659.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5209..5415
                     /locus_tag="LR726_001562"
     CDS             5209..5415
                     /locus_tag="LR726_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216660.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5956..7086
                     /locus_tag="LR726_001563"
     CDS             5956..7086
                     /locus_tag="LR726_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL6216661.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            7178..7933
                     /locus_tag="LR726_001564"
     CDS             7178..7933
                     /locus_tag="LR726_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL6216662.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            7991..10030
                     /locus_tag="LR726_001565"
     CDS             7991..10030
                     /locus_tag="LR726_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL6216663.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(10139..11290)
                     /locus_tag="LR726_001566"
     CDS             complement(10139..11290)
                     /locus_tag="LR726_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL6216664.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(11444..11974)
                     /locus_tag="LR726_001567"
     CDS             complement(11444..11974)
                     /locus_tag="LR726_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL6216665.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(12073..12423)
                     /locus_tag="LR726_001568"
     CDS             complement(12073..12423)
                     /locus_tag="LR726_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL6216666.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(12580..14109)
                     /gene="ahpF"
                     /locus_tag="LR726_001569"
     CDS             complement(12580..14109)
                     /gene="ahpF"
                     /locus_tag="LR726_001569"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL6216667.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(14119..14682)
                     /gene="ahpC"
                     /locus_tag="LR726_001570"
     CDS             complement(14119..14682)
                     /gene="ahpC"
                     /locus_tag="LR726_001570"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL6216668.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(14826..15488)
                     /locus_tag="LR726_001571"
     CDS             complement(14826..15488)
                     /locus_tag="LR726_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6216669.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            15672..17318
                     /locus_tag="LR726_001572"
     CDS             15672..17318
                     /locus_tag="LR726_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL6216670.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            17309..18391
                     /gene="uxuA"
                     /locus_tag="LR726_001573"
     CDS             17309..18391
                     /gene="uxuA"
                     /locus_tag="LR726_001573"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL6216671.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(18499..19937)
                     /locus_tag="LR726_001574"
                     /pseudo
     CDS             complement(18499..19937)
                     /locus_tag="LR726_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         6726 a         3482 c         3837 g         5892 t
ORIGIN      
        1 taaagtatat acaatcaaaa aaatcgtatt ggattttttt aaagaaaatg attatttgaa
       61 aaataaagaa atcattatcc cagaatttga ctcgcggaac atcttgctag ctttagtacg
      121 ttatgtcaat tgggaaggct atgaaaataa aaatccgtat gtagaagaag cttgtaacga
      181 gttgattgat tatgtagaga ctcatttttt taaaagaaga tattcggaag atgaaaagtc
      241 ctttatcata cgagaaatag agatcgcaat cggaagaaag gcatttcccc ttggactgaa
      301 agaagaagat aagaaaagcg cagaaacgaa gccgttattt caattagtct gtcaaggatt
      361 ggaagagcaa aaaggaattc tagacttaca agaagaagat gtctattata ttttttcttt
      421 gttcaacagt agaaattata ccaatgagaa catggagctg ttaagaaaag atttcgaagt
      481 tgtttataaa aattttgttc tcaatgacct atcactgaat gaattgattt tacagattat
      541 tagtatcgct gaaatcgacg atgcagaaaa ttttattttc aagcaatcct ttttacaatt
      601 tgtccgtacg ttgtgggcag atggacaagt atttttaccg gaaaggatct atcttctatc
      661 agaaaaagaa caacttctgt acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa
      721 aaatcaaatc caactaagat ggaatgaaaa tttcattcga aaattcgtca aaagtctttc
      781 tttgatcaac agtgaacaag cggggagaca ggaaatagaa atttttgttg tttcagaaag
      841 ttcagtcaaa caattttttt atcgaaccca atttcatttg tatgcggaag aaggcatcgc
      901 tgaaataaat cctattttat ttcgttcatt agaagaattg cctgatgaat gtctatgtgc
      961 gaaaaaaaga gtggttttgt gcgatgctgc tagctatcaa aatggattag aaactgaaaa
     1021 gacaaagatc tatccaatct cattaaaagg tgcaaacatc catttgtatc atattgctca
     1081 ggaacttcac ctattaaaaa aacatatttc ttgatgagac cttaattagg agaccttcta
     1141 caaagctttt taattgtctg tagaaggttt ttatttgagt cctgattcgt gcgaagatct
     1201 acttatataa caagctttct ctgaaatttt tttaaaatgc tattgactta gagtcaactc
     1261 taagtagtag aatagataaa gtgtaacaaa tctattttca ttaaaagaag atagtacgga
     1321 ggagagaatg tattatgagt acagtattac atgtattgga tgcaatcaat agccccgcag
     1381 atgtcaaaaa attatctgtt gaggaaatga aacagttggc agaagagatg cgccaaaaaa
     1441 tcttgcaaaa ggacagcgat gtaggtgggc acgttggtcc gaatctaggt gtagtagaat
     1501 taacgattgc gtttcattat gtatttgatt caccaaagga taaaatcgtt tgggatgttt
     1561 cccatcagtc ttatggtcat aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac
     1621 attaccgtga agtgagcgga tattccaacc aacatgaaag taaacatgac tattttacag
     1681 tggggcacac atctacatct atcagcttag caacaggttt agctaaagcg agagatctga
     1741 agaaagaatc aggaaatgtg gtagcattta tcggtgacgg ctccctaagt ggtggcttag
     1801 cttttgaagg attgaacaat ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg
     1861 ataatgagat gtctattgat gagaatcatg gcagtttata taaaaactta gctgagcttc
     1921 gtcaaaccaa cggtaaaagc agcaataacc tatttaaagc aatgggattt gattatcgtt
     1981 acgtggataa tggaaacgat ctggaaacgg tgatccgttt atttgaagaa gtaaaagata
     2041 ttgatcatcc gattgtactt cacgtccata cggaaaaagg tcgcggttat cagccggcaa
     2101 tcgataataa aatgaaatat cattggcatg taccatttga tctgtccacc ggggaggcaa
     2161 agcaagtaac ttctggagaa acatttaata tgatcatcat ggattatttg gataagaaaa
     2221 tgcaaacaag tacagctcct attgccgcag tcaatgcagc gataccaggc gcctttggac
     2281 tgaaaacatt ccaagaaaaa catccagatc attattttga cgtggggatc gctgaacagc
     2341 actctatcac tttcgcttct gctttggctt ctcagggagt acgtccagtc gtcttccaat
     2401 caagtacttt cttgcaacgg gcatatgatc aattatcaca tgatttagcg atcaacgaga
     2461 atccagtgat tattttagtc ggtggcggaa cgatctcagc tagtgaccga acacaccaag
     2521 gaacatttga cattccttac ttatcaagta tcccgaacat caaatatctt gctccagcaa
     2581 caaaagagga tatgatctct atgctagatt gggcgttcga acaaacagcg cagcctgtag
     2641 caatccgaat tcctacacat ggtgtagaac atggtacagc tatcgcaaat gattatagta
     2701 ttccagccta tcaaacagta aataatggag gaaaagtagc aattttagct ttaggcggtt
     2761 ttcttgaatt aggaaaagaa acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt
     2821 tgatcaatcc aggaacgatc acagaaaatg acgtagaaac attagataaa ttaaaagaaa
     2881 cacacgaatt agtcatcaca ttagaagatg gtagtttgtc aggaggattt ggcgaaaata
     2941 tcagtcgtta ttatggtgat tcttcaatga acgtattaaa ttttggtgca gagaaagaat
     3001 tcacggatag cgttcctgta gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa
     3061 tcgttgaaga tagtctgcgt gtcttgaata ttcaataatt cattatgctc accatccaga
     3121 taattgttta tactagtatc tggatggttt tttcattata aaaagcaaag gggtggatgt
     3181 tatgtatcag caagtagaag aatatgtcaa aaagttccaa gaaaaatatc ctagttatca
     3241 atcagaaaaa ttaggtttct tttcatttgg ggatacagaa aaaatggcag acgagttgag
     3301 cgaactagtt gtaaaaaaga taaagactgg tacatcaagt ggttacgatc tgtataaaca
     3361 agagaatgag cctttaccaa aagttggtca attagacgtg gtcctgaatg gaaaaaatga
     3421 accagtttgt atcatcaaaa atataaatgt gaccatcttg ccttttagtg aagtcacgaa
     3481 agagcatgct tggaaagaag gagaagggga tcggacatta gcttattggc ggaaagtcca
     3541 tgctgagttt tttaaacatg cttataaaga agcgttgaac atagaattta cagaagagaa
     3601 gctggtcgta tacgaagaat ttgaagtaat tttcttttag taagaaaggt ctgctgaaag
     3661 aaatagctca ttattgtctc ctgaatagaa aagggttaca tttttatatg aagctgtatt
     3721 tttatataac attactggag gagatattgt ggatacactg gaacaaatca aaggcggagt
     3781 cgtcgtttct tgtcaagctt tggaaaacga gccgctacac agttctttta ttatggggag
     3841 aatggctttg gctgcaaaag aaggcggagc agttggtatt cgagcaaata cgacagaaga
     3901 tatcaatgaa atcaagcgaa tcgttgattt accggtaata gggattatca aaagagatta
     3961 cgatgggtca gaagtgttta ttactgcgac agaaaaagaa gtggaagaac ttttggagac
     4021 gcaagcagaa atcattgcac tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac
     4081 tgctgaactt gtccaaatga ttcacgaaca cggtcggtta gctatggcag atatttcgac
     4141 ttttgaggaa gcgcttcaag cgcaagaaga tggttttgat cttttgtcta cgactttagc
     4201 aggctataca gaatattcac gaaagacgca gacacctgat tttgatttat tgaatcaaat
     4261 catagaacaa gtcgatatcc cagtaattat ggaaggacat actgactctc ctgaacaagt
     4321 agagaaagca tatgaattag gtgctttttc agtcgtagtt ggatctatta tcacccgtcc
     4381 gcaattgatc actaaaagat atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg
     4441 cacggcacag attcttgaca aaaaagaaaa attgatagat agtttaaagc agattctaag
     4501 aagatgaaaa aagcattact tttcattttg tcttcaagat ctgcttttta acgaatttta
     4561 tagaaagaag gcgaaaccat gtttgaacgg agtccttcag aattgacaga acgtgaaaat
     4621 tacaagttgc tgattggttc agtcatccct agaccagtag cagtagtaac gactaaatca
     4681 gcaaaagata tagtgaatat cgcacccttc agttacttca atatcgtcag ttcgaatcga
     4741 cccatcttat ccttggcgat ccagagaaaa caagatgcag taaaagacac agcaaagaat
     4801 attcttgaga caagagaagc tgttatccat atccttgatg aggacaacgt agaagaagta
     4861 aataaaacag cagccaatct gcctaatgag gaaagcgaac tgtacctgac tgacttgact
     4921 ttaactgatt cagtaatcat ttcagtacca gcattgacag aagcaaaagt tcgttttgaa
     4981 acatccttat atgaacatgt cgagatacgg aataaaaata aagtaacagc tgatttattt
     5041 ttgctagaag tacagaaata tcatttaaat gaaacagtct atgatgataa gacaggaagg
     5101 atcgatccaa gaaaattgaa tgctgtaagc cgtttggcag gaaatgatta tgcagcaata
     5161 ggagaaattt tcacgattgc acgtcccgaa taaaaaggag cactagttat gttaaaaata
     5221 agctataaac catcgacaga ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat
     5281 tttctgcaag ggcagtactt agaagttccg ccattgcaag accattttgt cgttactact
     5341 gttactttag atggtaagga aatagaaatg ccagatcaaa cgattagcgg attgtttaat
     5401 tactttaaca aataaatatt tattccgaaa agtgaaaaaa catataaaaa aacacataaa
     5461 actaaaaaat tatcaaagaa tcctggtgca actgaaagaa tttgcaacgg gattctttca
     5521 acttatttgt tatttttttc taaaaaatat taaagattgt taaatttcac tatttcattt
     5581 caaattccat tgcctaatca ttaggaaaag tctataatga actcgtaaat agaatatagt
     5641 aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga aacgtcgaaa
     5701 gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag ctaagaatgt
     5761 tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg tcagaattct
     5821 tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat ttgatgagac
     5881 aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag
     5941 atcgattggt ggattttggg tccttatttg actttgtcca tgatcggtct tttagaagta
     6001 tacagtgcca gttcttatcg tttattgcaa gccgatgaaa atacaaaaag tctattattg
     6061 cgtcaactaa ttttcatatt tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa
     6121 ctacactatt tacttcaccc taaaatagca ggatacggtt tagccttatc gattttcttt
     6181 ttagtattag taagaatagg gatattcggt gtcactgtca acggcgcaca acgttggatc
     6241 tctctgtttg gcattcaatt ccagccttct gaactggcaa atcttttttt gattttttat
     6301 ttaagctggt tttttcgtga cggaaatagt agcccaaaag atctaaaaaa accattcctg
     6361 attacagtag gtataacttt tctgatttta tttcagccaa agattgctgg agcattgatg
     6421 atcctttcga ttgcgtgggt catattttgg gcagcggcgg ttccatttaa aaaagggatc
     6481 tatctaatcg ttactttttc tgcattgctg attggagcag caggcggggt attatattta
     6541 ggaaataaag gttggcttcc acagatgttt aatcatgctt atgaaaggat cgcgacttta
     6601 agagattctt ttatcgatag tcatggtgcg ggctatcaaa tgacccattc gttttatgca
     6661 ctatataatg gtggcatatt tgggcgggga ttaggaaata gtatcacaaa aaaagggtat
     6721 cttccagaga cagaaacaga ttttattttt tcgattatta cagaagagct tgggctaatc
     6781 ggtgcattgt gtgtgctttt cttattgttc tcattatgca tgagaatctt ttgtttaagc
     6841 agtcgctgta aaaatcagca agctggattg tttcttttag gattcggaac tttgctattc
     6901 gttcagacga taatgaacgt gggaagtatc gcggggctga tgccaatgac tggtgtgcca
     6961 ttgccatttg ttagctacgg aggaacaagt tatctgatcc tttctttagg aatcggaatc
     7021 acgttgaata tttcttcaaa gatacaggcc gaagagttgg cgctctatcg acctgaaaaa
     7081 caataaagag aaactgagaa actttcagtt tgaattatca taaagtacaa tcttttattt
     7141 ttcaagcgag aggttctttt agaaagagga attcgcaatg aaagtatctc aaaaaatcat
     7201 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
     7261 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
     7321 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
     7381 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
     7441 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
     7501 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
     7561 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
     7621 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
     7681 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
     7741 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
     7801 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
     7861 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
     7921 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
     7981 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
     8041 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
     8101 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
     8161 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     8221 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     8281 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     8341 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     8401 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     8461 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     8521 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     8581 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     8641 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     8701 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     8761 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     8821 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     8881 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     8941 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     9001 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     9061 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     9121 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     9181 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     9241 ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg acaacggcac
     9301 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     9361 gggatcttat caagcaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     9421 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     9481 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     9541 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     9601 tggatatgga cagggcgaac ttctaattac tcctatccag caagcagcaa tgtattctgt
     9661 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     9721 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     9781 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     9841 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     9901 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     9961 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
    10021 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
    10081 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
    10141 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
    10201 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
    10261 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
    10321 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
    10381 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
    10441 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
    10501 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
    10561 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
    10621 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
    10681 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
    10741 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
    10801 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
    10861 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
    10921 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
    10981 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
    11041 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
    11101 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
    11161 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
    11221 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
    11281 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
    11341 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
    11401 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
    11461 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
    11521 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
    11581 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
    11641 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
    11701 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
    11761 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
    11821 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
    11881 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
    11941 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
    12001 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
    12061 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
    12121 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
    12181 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
    12241 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
    12301 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
    12361 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
    12421 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
    12481 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
    12541 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
    12601 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
    12661 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
    12721 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
    12781 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
    12841 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
    12901 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
    12961 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
    13021 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
    13081 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
    13141 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
    13201 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
    13261 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
    13321 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
    13381 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
    13441 agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag gttctttttc
    13501 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
    13561 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
    13621 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
    13681 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
    13741 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
    13801 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
    13861 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
    13921 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
    13981 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
    14041 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
    14101 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
    14161 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
    14221 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
    14281 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
    14341 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
    14401 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
    14461 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
    14521 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
    14581 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
    14641 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
    14701 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
    14761 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
    14821 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
    14881 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
    14941 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
    15001 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
    15061 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
    15121 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
    15181 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
    15241 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
    15301 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
    15361 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
    15421 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
    15481 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
    15541 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
    15601 gacatagcga tttttattgc ataaaattca cttgaataca aaaactaata tgttagttta
    15661 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
    15721 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
    15781 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
    15841 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
    15901 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
    15961 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
    16021 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
    16081 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
    16141 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
    16201 gtattcttgc tgtgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
    16261 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
    16321 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
    16381 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
    16441 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
    16501 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
    16561 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
    16621 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
    16681 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
    16741 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
    16801 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
    16861 atccaaatat tcctaatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
    16921 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
    16981 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
    17041 aaggaaaacc attcatcccg agtccagacc cactattgac agaccttcag cataagcttt
    17101 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
    17161 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    17221 aagaaatgtt catcaagatg gtagcaggac caaatgccgt acgaaaaaca ttacaggatt
    17281 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    17341 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    17401 cacattgatg gatataccag taggagaggt atggcctagg gagaggatca aagaactaaa
    17461 agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga
    17521 tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat
    17581 ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga
    17641 ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat
    17701 atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa
    17761 tgaatttgga ttgccgggat gggaacctga gcgcttagct cctatcaaat ccttgcttga
    17821 agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc
    17881 aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc
    17941 atactcgatt tttggattag ctcgaatcgt aaagaatcga gaagatttag attggttgtg
    18001 tcgcatagta gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga
    18061 tccagaaaat gatgtatatg ggattttagc agaattttgc caaagagatc gaatcccatt
    18121 tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg caccacataa
    18181 atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt
    18241 taacggatac atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg
    18301 atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat
    18361 cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa
    18421 taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat
    18481 ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag
    18541 tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc
    18601 ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt
    18661 caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat
    18721 cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc
    18781 acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg
    18841 tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg
    18901 ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa
    18961 tacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt
    19021 aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt
    19081 ttcaggtata aacaaatcaa atacagtaca aagggactcc accatttctc ttgaataaag
    19141 aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga
    19201 ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca
    19261 aattggcaga ttaggtcgca aaatatctac agagagatca ataaaaaagt caatatattc
    19321 atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat tctctttaca
    19381 tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt caagtacttc
    19441 ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat cataaaattc
    19501 tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt gaatttgtac
    19561 ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca aaccgtacta
    19621 tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac tatagtttgt
    19681 ttgctgactg aaactgataa tcgaatttgt ctgacagctg aacaaagaag tcaagatctg
    19741 ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt aagccggatc
    19801 aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca gttctgttag
    19861 attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat ttgattcaga
    19921 catatggaaa tgatctg
//
LOCUS       DAFGFZ010000052        18966 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000052 DAFGFZ010000000
VERSION     DAFGFZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.052"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            32..2185
                     /locus_tag="LR726_001575"
     CDS             32..2185
                     /locus_tag="LR726_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBL6216672.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2154..3569
                     /locus_tag="LR726_001576"
     CDS             2154..3569
                     /locus_tag="LR726_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBL6216673.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3602..4636
                     /gene="sppA"
                     /locus_tag="LR726_001577"
     CDS             3602..4636
                     /gene="sppA"
                     /locus_tag="LR726_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL6216674.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4638..5159
                     /locus_tag="LR726_001578"
     CDS             4638..5159
                     /locus_tag="LR726_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL6216675.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5156..5542
                     /locus_tag="LR726_001579"
     CDS             5156..5542
                     /locus_tag="LR726_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL6216676.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6038..6110)
                     /locus_tag="LR726_001580"
     tRNA            complement(6038..6110)
                     /locus_tag="LR726_001580"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6075..6077),aa:Lys,seq:ctt)
     gene            6491..7060
                     /locus_tag="LR726_001581"
     CDS             6491..7060
                     /locus_tag="LR726_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL6216677.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7047..7907
                     /locus_tag="LR726_001582"
     CDS             7047..7907
                     /locus_tag="LR726_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL6216678.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8097..8801
                     /locus_tag="LR726_001583"
     CDS             8097..8801
                     /locus_tag="LR726_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6216679.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8806..10641
                     /gene="walK"
                     /locus_tag="LR726_001584"
     CDS             8806..10641
                     /gene="walK"
                     /locus_tag="LR726_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL6216680.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10638..11954
                     /locus_tag="LR726_001585"
     CDS             10638..11954
                     /locus_tag="LR726_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216681.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11955..12824
                     /locus_tag="LR726_001586"
     CDS             11955..12824
                     /locus_tag="LR726_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL6216682.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12879..13688
                     /locus_tag="LR726_001587"
     CDS             12879..13688
                     /locus_tag="LR726_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL6216683.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13791..14420)
                     /locus_tag="LR726_001588"
     CDS             complement(13791..14420)
                     /locus_tag="LR726_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL6216684.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14590..15882
                     /locus_tag="LR726_001589"
     CDS             14590..15882
                     /locus_tag="LR726_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6216685.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15958..16620)
                     /locus_tag="LR726_001590"
     CDS             complement(15958..16620)
                     /locus_tag="LR726_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL6216686.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16845..17129
                     /gene="groES"
                     /locus_tag="LR726_001591"
     CDS             16845..17129
                     /gene="groES"
                     /locus_tag="LR726_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL6216687.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17180..18805
                     /gene="groL"
                     /locus_tag="LR726_001592"
     CDS             17180..18805
                     /gene="groL"
                     /locus_tag="LR726_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL6216688.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6459 a         3057 c         3929 g         5521 t
ORIGIN      
        1 aaaatttttt agaaggagtt gtgttagata agtgataaaa tcatttcctt ttgtgcagca
       61 acaagatgaa aaagattgtg gtgttgcttg cctttcgatg attttaaaat attttaaaac
      121 tgatatcccg attcataaat tacgggacct atctgggact gatttagagg gtacttcagc
      181 atttggattg aaaaagacat ttgaaaaatt gtcttttgat tgtatggcta ttcaagcaga
      241 tagtgagatc tggaaagaaa gagatcttat cttaccatta attgcacata tattggttga
      301 taacagctat atgcactacg ttgtaattta taaaacgaag ggaaacgtac tatggattgc
      361 agatcccgct aatggaaaaa atagaaaaac gattgaagcg tttcaaaaag aatggacagg
      421 tattctttta ttacctatgc ctagagaaac ttatattcct attaaagaaa aagtagctgg
      481 attaagttct tttttgccta taatttggaa acaaaaagga ttagtattcc acatcgtttt
      541 agcatcgatt ttcattacgt tttttggcat aggaagctca tattattttc aaggtattct
      601 tgattttttc atccctaatc aagcacgttc aactttaaat ataatttcca ttggcttgat
      661 cgctgtctat ttatttcggg tattgtttga atatagtcgt agttatcttc ttattatttt
      721 aggacagcga atgagtatgg caattatgct tcagtatttc aaacatgtgt taactttacc
      781 aatgaatttt tttgctacga gaaaatcagg ggaaattatt tctcgttttc ttgatgccag
      841 taagattatt gatgctttag caagtgctac gttatcaata tttttagata ttggtatggt
      901 gttgcttgtt ggggttacgt tagctattca gaatagtacg ttgtttttaa tcactctatg
      961 ctctttgcct ttttatgtag ttgcaatatt cgctttcgtg aaaagctatg aaaaatcgaa
     1021 cagggaagaa atggcagccg gtgcagctgt aaattctagt atcatagaaa gtcttaaagg
     1081 aatagaaaca attaaagcat atagcggtga agagaaagta tataaccgta tagaccatga
     1141 atttgttaaa ttaatgaaaa aatcatttcg tacggttacg cttgataata ttcaacaagg
     1201 aattaaacat ttgattcaat taattagcag tgcattaatt ttatggctag gttcttattt
     1261 tgttatggat aatagaatta gtctaggtca acttatcaca tacaatgctt tactagtatt
     1321 tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa cggctcaagt
     1381 tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga accagtcttt
     1441 tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa acgtttcatt
     1501 ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc cttcgcacag
     1561 taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa agttatttgt
     1621 tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc acttagacat
     1681 tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat ttttctttag
     1741 tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt tcgaacaaat
     1801 aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc cattacgtta
     1861 cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac aacgattagc
     1921 cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag caactagtgg
     1981 attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa aagaaaaaac
     2041 gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa ttttagttct
     2101 ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg ccgatggtat
     2161 ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat aataattggt
     2221 tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg gtgttatacc
     2281 ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa aaagaggttg
     2341 ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa gtgccattag
     2401 aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag gacgatgtgt
     2461 tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa caagaaaacc
     2521 aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata agtaaaggaa
     2581 aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag agcatgttgt
     2641 cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat caaaagcaat
     2701 tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt tcagatagta
     2761 aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca ttaaaagatt
     2821 tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat aatgtttctg
     2881 atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa caaattgagc
     2941 aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt aaaccaacta
     3001 cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta gaacaagcaa
     3061 taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag aaccagtcag
     3121 ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct gtatctggaa
     3181 cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga acagtgcttg
     3241 cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt ttacctgcga
     3301 atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat caaaaaggtg
     3361 gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct actgccactg
     3421 agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg tttccgtttc
     3481 gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat tggcaacaaa
     3541 ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag gaggagttta
     3601 tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt tactatccat
     3661 tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa ccctaacagg
     3721 aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag aagaaggcgc
     3781 atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata caggtgaaag
     3841 caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc taaagaaaat
     3901 tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg ggggaggaat
     3961 atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag atattcctat
     4021 ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg ctggttcaga
     4081 taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta tttctggttt
     4141 aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata aaagtggtgc
     4201 attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata aagttataca
     4261 ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag gaagaaatat
     4321 ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta accaagcagt
     4381 agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt ccctaaaaaa
     4441 agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa ctggctttgc
     4501 ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa ctacatcaga
     4561 tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac caatgtatta
     4621 ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct aattatgtat
     4681 ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt attagcatca
     4741 tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct gacaaatcca
     4801 ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt gtacttttga
     4861 ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga gtagtgagct
     4921 ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata tgtaggttta
     4981 tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg cccaagaaac
     5041 aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca ttagaagcta
     5101 ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga attaaatgaa
     5161 taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga ttttatgtag
     5221 tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta ctttaaatac
     5281 tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga atgttgtttt
     5341 atttggagga attaccctag ttttttcgat aatgattttc ataataggtt ttttagtaag
     5401 aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta ttattccatt
     5461 ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg cgggattgat
     5521 gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg attaatcaat
     5581 taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac atgtagataa
     5641 aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact ctctgattgt
     5701 tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta aatattcttc
     5761 actttttaac cttttatagt acttagattt aatcctatat gtttagagag ttcattggca
     5821 gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact gttcataatt
     5881 atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt aaaaataaaa
     5941 agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga aatgctttcg
     6001 caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc
     6061 gcgacccgct gattaagagt cagctgctct accaactgag ctaacggccc gtacaaaaag
     6121 tacttaataa tagtagcact aagaatttta aaatgcaaga ctacatttaa agaaaatcag
     6181 aaaaatcttt acttccttat aggaaatcat tcatagatta ttcacatttg ctcgctatta
     6241 tataagcata ccaacaaaca aagttcttgc aagagaacaa accctaacac ttttttcatt
     6301 ttttactcct ttcccgccgt ctccccggcg ggtctcctgt tcttaacgat aaaagacaag
     6361 aaggcttagc cttcttgttt tttgttttca tggaatcccg ctaatcaaat cacctagtta
     6421 aagcaactag attgaatgtt tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg
     6481 agcggttatg atggaaaaaa tgcgtcagca attaatggag tgggggaagg aactagaaac
     6541 attccgtctg cctcactggg aagaactccc tgacttagaa ctttatatgg accaagtcag
     6601 aacgttagtt gatcgttatc tttcacctgt gatacaagga gaaaagcatc cattgcttac
     6661 ctcttctatg gtaaacaatt acgttaaact aggtttgata ccagcaccag ttaaaaaaag
     6721 atataacaaa gaacacgtag catttttact tgcaatcact acgttgaaac aagtattgac
     6781 tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc
     6841 ctataatcta ttttgcgatg aacaagaagc agctgtgtgg atggtcagcc aattagcaca
     6901 aggtaaatct catccgcaaa aattcgacca ggcaacccct gtagaatata ttgctgtaag
     6961 agcggcaact ttatcgtttg caatgaaact tcttgcggaa aaaacgattg ttctagaaac
     7021 cgaatattta aaagaggaga aaacaaatga aaaacaataa aatcgcaatt ttagtagatt
     7081 caggtacgga tgttccagaa gaattgataa aaaaatacga gatgtatatg atcccattga
     7141 aaattattta taaagatcag gtatatacag ataaaattga cattacccct gaagagatct
     7201 atcaacgttt gccagaggaa atccctggta catctttgcc ggatggtgag tcgatcacag
     7261 aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt acttgcggta acaatttcca
     7321 gtggattgag cgggacatat aatgtggttc gtttaattgc acaacagcaa gaagaattgg
     7381 aagcatatgt actagatacg aaaaatattg gtattggtgc aggtttttct gcgatccaag
     7441 cagcaaaatg gctggaagaa ggagtagaat gggatcaact gatcagtaat ctgaatgagt
     7501 tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg
     7561 gacggatcgg tttagttgct tctattgtag gaaccgcttt aaagcttaac ccaatcatct
     7621 catgtaatga agaagggatt tattacacag taggaaaagc taggggacgt aaaaaaagtt
     7681 tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag
     7741 ctgttgccca tggggatgcg aaagaagaag caattgaaat ggaagcgaga cttaaaagag
     7801 agttcccaca agcaaaccaa atctactttg gccagatttc tccggcatta gtagtccaca
     7861 ctggtccggg attattgggt gtcggtgtac aagttttaga aggataaatc aaaaaaagga
     7921 aacactgagg tgaaaatcag tgttttcctt ttttattgaa gttagaacaa aaaataataa
     7981 agtattttgc actttcattt taattttctc ttttgtttta taggcaaaga tgaaacaaaa
     8041 gcagaaatac ggtataatga gaaggtatga caataatata taagaggtgg ctctttatga
     8101 aaaaagtttt agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag
     8161 ccaaagaagg ctatgacgtt tatactgcat atgatgggga agaagccttg gaaaaagttg
     8221 ctgaggtaga acctgatttg attttacttg atttgatgct acctaaaatg gacggcttag
     8281 aagttgctcg tgaggtacgc aaaacttatg atatgccgat cattatggta acagccaaag
     8341 attctgaaat tgataaagtc ctaggcttag agttaggcgc cgatgattac gtgaccaagc
     8401 cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg
     8461 ctgcgaagga accagaagag gcagcacctg cggaattgac gattggtgat ttgacgatcc
     8521 atccagaagc ttacatggtg acgaaacgag gagaaacaat cgagttaaca catcgtgaat
     8581 tcgaactact cttttacttg gcgaagcacc ttggacaagt gatgacaaga gaacatttat
     8641 tacaaacggt ttggggttat gattattttg gagatgtacg aacagtggat gtaactgtac
     8701 gtcgtttgcg tgaaaagatc gaagataatc caagccatcc aaattattta gtgacacgtc
     8761 gtggtgtcgg ttactatttg cgtaacccag aacaggagta attggatgaa aagaaaagtc
     8821 cgcttttttc gatcagtcaa ttttaagatc gctattacct ttattctgat tttattgatc
     8881 tcgatcgaaa ttattggtgc ctactttata cgtggtttag aacgttccac gatcaataca
     8941 tttattaaag atatgaatca aacggtggaa tcattagcaa caacaatcag cccggaattg
     9001 aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga atatcaaaag atttattgaa
     9061 aacagtgcga ctagcgatat catcgaaata cgggtcgttg acgaaaaagg gatcatccga
     9121 ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga aaaatgatta tgttgatatc
     9181 aatgatttca cgactaaacg ttatgttgct ttagataacg acgataaacg ggtgaatatc
     9241 aatgttcaac cgatcctgtc cccgaccggc gatgccgtta tcggggctct ttacgtcaaa
     9301 agtaatatcg aacaaaaata ttcggaaatc aataacacag cagtcatttt ctttactgcc
     9361 tcactaattg cggcatttat ttcaatgatc gtatctgttt tagttgctcg ttcgattaca
     9421 cagccgatcg gtgaaatgag agaacaagcc attcgaatcg ctagaggaga ctacagtcgg
     9481 aaagtcaaag tgcatggaca agatgaactt ggacagctgg ctgatacctt caatcagtta
     9541 gctgaacgta ttgaagaagc tcaagataca atggaagctg aacgcaatcg tttggatagc
     9601 gtgctatccc atatgacaga cggcgtcatt gcgacagatc gcaggggtaa agtcattacg
     9661 atcaatgata tggctgtttc cttgctagat atcaaaaacg aggaagcaat cggacaatcg
     9721 attttgacac ttttagatat cgaagaagaa tatacgttgc ggaaattatt agaagatcca
     9781 aacgaaatgg tacttgaccg ttcaaccagt acattggaaa gtgatcagat gatcttgcga
     9841 gtagactttt ctatgatccg tcgcgaatca ggttttatca gcggattagt agctgtcttg
     9901 catgatgtga cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt
     9961 tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc attgagcgaa
    10021 ggtgcgtgga aagacgagga agtagcacca aattttttga aggtaacgct agatgaaaca
    10081 gaccgaatga ttcgaatgat caatgacctc ttaaatctat ctcgcatgga tacaggtaat
    10141 acccagcttc aattagaata tgtcaatttc aacgagttag tgaattttgt tcttgaccgg
    10201 tttgatatga tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga atttacgcaa
    10261 agagatcttt gggtcgaagt agatacagat aaaatcatcc aagtagtcga taatatcatg
    10321 aataacgcga tcaagtattc accagatggg ggaacgatta cttgtcgctt attagaaacg
    10381 cataataacg tgattttaag tatcaccgat caaggattgg ggattcctaa aaaagatctg
    10441 aaccgagtgt tcgagcggtt ctatcgagta gataaagcac gtgcaagagc acaaggtggg
    10501 acaggacttg gattagctat ttctagagaa gtgattaagg cgcatcgagg cgctatttgg
    10561 gcagagagca aggaaggaaa agggtcgact ttttatatct cgctgccata tgaaccttat
    10621 gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga ctcattttga
    10681 tggttgcgtt aagcatatac ttctcttatg caatctggct gagcccagca ggaaaaagtt
    10741 caatcaacgt agatgagaca aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt
    10801 ccgaagtctt tcttccgctt catgttacat ggcaccattc gggaatgatc caagaaacca
    10861 acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga taaagcaaga tttggaaagc
    10921 tttctgaagt agtttcagga gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg
    10981 gttttgagtt ggcctataat gcgccttttt tgctaagaga atacaaggaa gcatttgatt
    11041 tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt actagaatac
    11101 aattcgatca aggaaataaa aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg
    11161 aagcaaatat ttcgttgaat tggacagata tcaataaaga attaacagca aaagacacaa
    11221 aatggacaga aatgacaaca gaaccatcga cgatcgacac gcaatatgct acgaaacatt
    11281 cgatcaaact gaagaagtat agttatatcc tgtcacagca accctataca ctttttagaa
    11341 atgctttttt ccagaaacca gatagcgtga aaaataatga tgaaagcaat gaattgattt
    11401 tttacggtgg gaatgaaaca atgtctatcc attcggaaac gcaagtagtt gattttcgtg
    11461 gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc ccctatatta
    11521 gcaaattagg cagttcttta gggaacctca gatattttga ccgcagtgac aatacgatcg
    11581 attatcggat attcgtggaa ggcttcccgg tttttgggac agacagcaga ggacagattg
    11641 gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa accagtttga
    11701 atacgattca agtgccgata ccttcagacg aagaagtaga acttgcaagt acgaatgaaa
    11761 tcatccagca gttaaaagct aagggagcag atagcaacaa gattggttca gtgatcgttg
    11821 gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta cctgaatggt
    11881 atgtgaaata taaagataac tggtattcag ctaacgacct tctggaaaaa ttacctggat
    11941 tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag
    12001 ggttgaatgt gtttttattc agcagttatc atgacgcggt ataccaagaa aacaatgtca
    12061 ttcgctctaa tcagactgaa tcaatccaac aacgtctggc tagcgacgat atcacttaca
    12121 cggggaagat ttcgtctgag aacaagcaag gctattatct tagcgcggag cagaccaatc
    12181 ttagcacttc attagcgaat tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg
    12241 tgtttttaga cggtgatgtt ttgacgaaat ctttatccag ttcagatgct gaaaagaacg
    12301 tgatcgatcc agataaaatt agtagttcct tggaagattt tttatctaac aaagacaata
    12361 ttttattcgg taatgattat gtgtacacga aaaacttttc tcatttagaa gaagaaccac
    12421 tagaaatcca agcaagccaa gagtatgaag gcatcccatt caatgatgat acagcaaaaa
    12481 tcgttctttc tttagaaaaa aacgataatg attatagcat cacgaaatac atgcagacgc
    12541 atttatctga tatcgagcag ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa
    12601 tcaatacatt gtacatcaat aacaaaatac caagaggatc aaaaatttta tggcgtcagc
    12661 tagcttattc ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg
    12721 tagcgatcga aacaagcgat aaagtggtcc aaattgagag cgtcaacgct ttcagcaata
    12781 cgatcgtgac aaacaatact gtaccaaaag tggaagatca ataaaaaagc gagtataatg
    12841 aaaaaagttt acaaagtaag aagggaagta ggagtcaaat ggctgaagaa aaaccagctt
    12901 ttaaaatcag tatcctagca agtggaagtt cgggtaattc actttatatc gaaagtgaaa
    12961 aaaaacggct tttggtcgat gcaggactta gcggtaaaaa aatcacttcc ttgatggctc
    13021 aaattgatcg gacaccggac caattagatg gaatacttgt gacgcatgaa catcgagatc
    13081 atatacatgg cgtgggtgtc ttggctcgta agtatcattt ggatgttttt gccaatcagc
    13141 agacttggga agcgatggaa ccactgattg gaaatgtacc agtggaacaa aaacatctat
    13201 ttgagatggg gaaagtcaga acattcggcg atatcgatat tgagagtttc ggcgtctccc
    13261 atgacgcagc ggctccgcaa ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt
    13321 tgacggacac cggatattgc agtgattata tgcgaggtgt cattgaaaat gccgatggtt
    13381 atttgatgga aagtaatcat gatcttgaga tgttacgcat gggaccttat ccgtggaatt
    13441 taaaacagcg tattttagga gatagaggac atctttccaa cgaagacggt gcacttgtca
    13501 tgactgatgt gatcggtaat cggaccaaac gaatctatct tggccatctt agcaaggaaa
    13561 acaatatgaa agaacttgct catttaacaa tggaaaatgt tttgaaagaa aaagattttg
    13621 gcgttggaca tgattttgaa atctatgata cagatccaga tgaagcaact gagctatttt
    13681 cgatttaaac agttcaatca ataaaaatat aaaaagcaga agataaaatc aatcagggtt
    13741 ggttttatct tctattttta tttttttagc atattaaaaa aataaccgta ttatcgactg
    13801 actacactga tgtgctgctt ttcatgtttc tcatccagta tctcatctac taatgcaatc
    13861 gcgtaatctg ccatgctgat cgtactgtcg ctagcagcgt ttctttgtaa ataatcatca
    13921 cttaacaaat aagtacctgt acgtttgcca tgtggatcaa ataaagaagc tggactgata
    13981 taagtccaat gtagattttt agattgtttc aattttaaca atccttcgta catattgaaa
    14041 gctgttggat agaagggtgc atttttaggt acttggtcaa tcatacgagt cgtcttttct
    14101 gtatctaaga ataacgaaga ggcaccccca acgacaatta agcgtgtatc ggttccttca
    14161 agcatctcaa tcaaatggga caaagaagtt tgatgcattt cttcttttcc tttgggagcg
    14221 ttgaaagcat caacgacaac atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa
    14281 tccttttgta taaacggaac atctgatggc aatttttctg gatgacgcac gattgccgtc
    14341 acgtctaaat ctctggacaa tgcttcgtct aaaataaatg aacctgcatg gccagtagct
    14401 ccaatgagtg caattttcat aaaaaacctc cttttttatt cctgtccatt gtaagcagtt
    14461 gctgaatatt cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc ctttcatggt
    14521 tcgtgatcat tgggtatagt taaattaaga aaactgattg ataaaaatag aagttgaagg
    14581 agataagaaa tgaaagagtg gttagatcgt gcctttttac caatgataac atctgttgtg
    14641 aatcttagac ccttaactgc tttgcgtaat ggaatgactt acacgatccc aataattatt
    14701 gttggctcgc tgtttttctt atttgctcat tttcctgtgg attacatcgc tcagtggata
    14761 gacaaaacag gattttctat gtattttaca caagtatacc aggctacatt tgatgtgata
    14821 gccttttgga tcgtttttgc cattgcatac gcttatgcaa aagaagaaga catcgatcgc
    14881 ttaccagtcg gcataacagc cttgtctgct tatctactgc ttattcagcc actaaatact
    14941 cagatagata ctgtctggac aggtgctgaa gggatactag gagcagttac aactgggctc
    15001 atcatcggat ggggctatgc acgtttagta agacttgcga aagggtggaa gcaaagagag
    15061 aagatacctg aaaatgtttg gcagtctttt acttcattga ttccaatcat tgttatagtc
    15121 acttttacgc ttatcggttc agcggttttt caacatttgt tcagaatgac tgttgttcaa
    15181 ggagtatgga catatgtcca gcttcccatt caatcgttca tagacacgtt gtttggtgtg
    15241 cttttattag gtttttttgt tccgttttta tggttatttg gtcttcatgg atctgcgatg
    15301 gtcaatgggt tggttagtcc cgtcttgcaa gcgaattcat tagcaaatgc agaaatctta
    15361 gcctcaggac aagagttgac ggtggcaaat ggcggacata tcgttactca gcaatttctc
    15421 gatcagttca tgacggttac gggtgctgga ttgacgttag gggctgtatt ttttatgacg
    15481 gtttttgcga aatcgaagaa atacagagaa ttaggaaatc ttgctctttt accagccttt
    15541 ttcaacatca atgaatcaat tattttttcc acaccgatcg tgatgaaccc gatgatggct
    15601 gttcctttta ttttcgcacc ggtcgtttca ggattgatta cctattccgc tttatatttt
    15661 ggattcgttc ctttatttac agcagtccaa gtcccttgga caacaccgcc gatcctctcg
    15721 ggtttcttag taggcggagt gcctgcagcg attttacaag gtatcgtgtt gtcaattagt
    15781 tttttcattt atttcccatt tttgaaaaaa atagattctg caaactgcaa aaaggaaaga
    15841 cagacaaaaa atgatggagc agcagaaaag atgaacgact aaaaaacaaa aatactcctg
    15901 tcgctttaga ttgggaaaat caccaatcta ggatgacagg agtatttata gtcatgttta
    15961 tactagccct ttttggacag ctaattgaac aagaacaact aatgtgttca ttcctacgtg
    16021 tgtgatcatc gatgtccaga tccgaccagt tgctttgtac tctaggctga agaagaaacc
    16081 taggaaaaag tagattagca gatgaccatc attatgagca aacgcaaaca gcaaagagct
    16141 gatcactgct ccgacccaaa aatttgaata tcgactgatg atccccagta tactgcggcg
    16201 gaatacgaac tcttccatga tgggtccagc aacagttgtc gccaaaataa aagcgggtgc
    16261 ttccatgatc atctggatga tattttgtgt attctcggaa ggggttgctt tgccaaacaa
    16321 tacagattct aacatgacag cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa
    16381 tccataaata acaatctttg gagcagaagc tttttttacc gctgattcga tcgctaaggg
    16441 ttcacgttgc ttaaaataaa aaatagctag gacaacggcg cctagtatat acatgactgc
    16501 agttgctgtg atcaattcag acgtagattt tacaagtcct atcgaagcta ggatcaaggg
    16561 tgagataaaa attagaccat agcaaaaaat gctgaggaag ctatattttt ttaaagacat
    16621 gtaattcctc cttttaaggg ataacaagca tgcaaggcaa gcttatatcg attgatttca
    16681 attatatcat aatttcaata agcagctgtc agaataaaat ttcttttctt tgatttaaaa
    16741 gcttgcaaaa aagagggaga atgagtatta ttaaaaatgt aggttagcac tcgaaggtta
    16801 agagtgctaa cagattaaaa aaagagttat cggagggatt tatcgtgtta aaaccattag
    16861 gtgatcgcgt cattatcgaa gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt
    16921 tagcttctgc tgctaaagag aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc
    16981 gtttgttaga aaacggtgaa aaagttcctg ccgctgtaaa agcaggcgac caagttatgt
    17041 tcgaaaaata cgccggtaca gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag
    17101 gaaaagatat catggcgatt gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa
    17161 tcaatgaggt gaatcaatca tggcaaaaga attgaaattt gcagaagacg cacgcgcagc
    17221 tatgttacgc ggggtagata aattagcaga tacagtgaaa gtaacattag gacctaaagg
    17281 ccgaaatgtc gtactagaaa aatcttacgg ctctccattg atcacaaatg atggtgtgac
    17341 gatcgcaaaa gaaatcgaat tagaagatca ttttgaaaac atgggtgcaa aacttgtatc
    17401 agaagtagca tcaaaaacaa atgatattgc aggagacggt acaacgacag caactgttct
    17461 gacacaagca atcgttcgcg aaggtttaaa aaacgtaact gcaggtgcta atcctctagg
    17521 cattcgtcgc gggatcgaat tggcaacaaa agcagcagtc gaagaattgc ataatatctc
    17581 aactgttgtt gattcaaaag aagccattgc acaagtagca gctgtttctt cgggttctga
    17641 taaagttgga catttgattg ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac
    17701 aatcgaagaa tcaaaaggga tcgaaacaga actagatgtt gtagaaggta tgcagtttga
    17761 tcgtggttac ttatcacaat atatggtaac agataacgac aaaatggaag ctgttctaga
    17821 aaacccatat atcttgatca cagacaaaaa aatctctaac atccaagata ttctgccatt
    17881 gttagaacaa atcttgcaac aatcacgtcc attgttgatt attgctgacg atgtagatgg
    17941 tgaagcattg ccaactcttg tattgaacaa aatccgtgga acattcaacg tagtagctgt
    18001 aaaagctcct ggtttcggcg atcgtcgtaa agcaatgctt gaagatatcg ctattttgac
    18061 aggtggtaca gtaattactg acgatctagg tttagaattg aaagatgcaa caatcgaaaa
    18121 cttaggaaat gcttctaaag ttgttgtaga taaagacaat acaacgatcg tagaaggttc
    18181 tggagaaaaa gaagcaatcg aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac
    18241 aacttctgat tttgaccgcg aaaaattaca agaacgctta gctaaattag caggtggtgt
    18301 agctgttgtt aaagttggtg cagctactga aacagaattg aaagaattaa aattgcgcat
    18361 tgaagatgca ttgaatgcta cacgtgccgc agtagaagaa ggaatggtct caggtggtgg
    18421 tacagctcta gtgaacgtta tcagcaaagt ttctgctgta gaagcagaag gagacgtggc
    18481 aacaggtatc aagatcgttg ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa
    18541 cgctggttat gaaggatcag tgattgttga taaattgaag aacgtggaac taggcactgg
    18601 tttcaatgct gctactggag aatgggtaaa catggttgaa gcaggtatcg tagacccaac
    18661 taaagtaaca cgttctgcct tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac
    18721 agaagcagtc gttgcagata aaccagaacc agcagcgcca gcagctcctg caatggatcc
    18781 atcaatgatg ggcggtatga tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta
    18841 gtgaaagaca gataaaaaga ggctggaaca gaagcgttta actccaagaa ataagaagaa
    18901 attcacggaa attgcttttc aaatttttgt gaatttcagc ttatttccga aggagttgct
    18961 tctgct
//
LOCUS       DAFGFZ010000053        18551 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000053 DAFGFZ010000000
VERSION     DAFGFZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.053"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            62..1018
                     /locus_tag="LR726_001593"
     CDS             62..1018
                     /locus_tag="LR726_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL6216689.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1091..1207
                     /locus_tag="LR726_001594"
     CDS             1091..1207
                     /locus_tag="LR726_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL6216690.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1262..2944)
                     /gene="recN"
                     /locus_tag="LR726_001595"
     CDS             complement(1262..2944)
                     /gene="recN"
                     /locus_tag="LR726_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL6216691.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2962..3411)
                     /locus_tag="LR726_001596"
     CDS             complement(2962..3411)
                     /locus_tag="LR726_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL6216692.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3518..4336)
                     /locus_tag="LR726_001597"
     CDS             complement(3518..4336)
                     /locus_tag="LR726_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL6216693.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4345..5235)
                     /locus_tag="LR726_001598"
     CDS             complement(4345..5235)
                     /locus_tag="LR726_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL6216694.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5237..5461)
                     /locus_tag="LR726_001599"
     CDS             complement(5237..5461)
                     /locus_tag="LR726_001599"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL6216695.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5466..6803)
                     /locus_tag="LR726_001600"
     CDS             complement(5466..6803)
                     /locus_tag="LR726_001600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL6216696.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6804..7658)
                     /locus_tag="LR726_001601"
     CDS             complement(6804..7658)
                     /locus_tag="LR726_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL6216697.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7755..8204)
                     /gene="nusB"
                     /locus_tag="LR726_001602"
     CDS             complement(7755..8204)
                     /gene="nusB"
                     /locus_tag="LR726_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL6216698.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8197..8622)
                     /locus_tag="LR726_001603"
     CDS             complement(8197..8622)
                     /locus_tag="LR726_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL6216699.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8868..9932)
                     /locus_tag="LR726_001604"
     CDS             complement(8868..9932)
                     /locus_tag="LR726_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL6216700.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10104..10349)
                     /locus_tag="LR726_001605"
     CDS             complement(10104..10349)
                     /locus_tag="LR726_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL6216701.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10488..10862)
                     /locus_tag="LR726_001606"
     CDS             complement(10488..10862)
                     /locus_tag="LR726_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL6216702.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10945..11859)
                     /locus_tag="LR726_001607"
     CDS             complement(10945..11859)
                     /locus_tag="LR726_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL6216703.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11880..12683)
                     /locus_tag="LR726_001608"
     CDS             complement(11880..12683)
                     /locus_tag="LR726_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL6216704.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12716..13705)
                     /gene="manX"
                     /locus_tag="LR726_001609"
     CDS             complement(12716..13705)
                     /gene="manX"
                     /locus_tag="LR726_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL6216705.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13808..14299)
                     /locus_tag="LR726_001610"
     CDS             complement(13808..14299)
                     /locus_tag="LR726_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6216706.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14490..17297)
                     /locus_tag="LR726_001611"
     CDS             complement(14490..17297)
                     /locus_tag="LR726_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL6216707.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            17943..>18551
                     /locus_tag="LR726_001612"
     CDS             17943..>18551
                     /locus_tag="LR726_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBL6216708.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVD"
BASE COUNT         5362 a         4037 c         3065 g         6087 t
ORIGIN      
        1 ttccatgtat tccaattccc tatccgttat aataaaagaa acaggcaaca ggaggaaaat
       61 catgatcaaa tattttacat tagataatga gaaactgcgt aataccggaa aggaaactga
      121 ccaaacgatc tggtattgtg tagaacgtcc taatcaagaa gagattcatc agctgacaca
      181 tcaatttgaa attccgtcag attacatcac ttccatattg gacgatgcag aaaattcccg
      241 tacagaagaa ttcaatcaat caaagttgac aaagccagcg ttattattgt tacaatatcc
      301 ttttgctaaa acaagtccta gcggttattt tcaagtggat acatacccgc tctctattat
      361 tttgacgccc aaaaagaagc ttatcacaat tacgaatcat gaaccactgt ttttaaagaa
      421 agtctttagc cagacctttc caaaaagtga tatttcgcct aatctcaata tcttctttca
      481 attcatttgg caggtcacac tctcttacaa cagttattta gctacgcttc aatctcaatg
      541 cgatactttg gaagccaaac ttcaagtatc cactgaaaac aaacaacttt atcaaatcat
      601 ggatatccaa aaaagcctcg tttatttcag agaagcgact tctgctaatt tagatgcatt
      661 gaaagaacta gcgcaaaata aacttttcca agaaaatcat gctttgaaaa atcatttacg
      721 tgatgtattg gtggaaacag agcaggcaat gactacttct cgcattcaat taaaactagt
      781 agaacaaatg aatcagactt tttcagcaat cgtatcgaat aatctgaata atattatgaa
      841 aattttaact tctttaacga ttattttaac tataccgaca atcgtcggca gtatctatgg
      901 aatgaacatt aagttaccta ttgctgaaag ggatgatgcc tttatatggc tagttctatt
      961 aacattattt atttgtatcg taactaccta ttatttaaaa aaagggaagt ttttataaaa
     1021 aagccctctt gtggaaatgg aaaaaagatt gtacacttga atcaactaat taatgacgag
     1081 gttgaaaaca atgcgtgaaa aaaaacaaac aagtactttt gctaaagtaa caaaatttgt
     1141 catttggaca atgctgattt taacgattgg atctgttgta ttgactgctg taatgagtgt
     1201 catgtaaaaa taaaatatag ctatcagctg ctgaaaagga cctgaaccac tgcgaggtgg
     1261 ctcaggtcct ttttgcgagt ttcaatagtt cttttgcatg ttcgagtgtg agttttgtaa
     1321 tctcacttcc agccagcata cgtgcaattt cttcttctct ttcttttgtt ttcaactctg
     1381 aaacagaagt ttgcgtacgt ccatcaatca ctgctttcac aatataatat tgatagtcgg
     1441 cgactgcagc aacttgaggc agatgagtga tgcacaaaac ttgagagtat ttcgatattt
     1501 gagaaatttt atctgcgatg gcctgtgcaa ctcttccgct cacacctgta tccacttcat
     1561 caaaaataat acttgtcaca ccttgctcag aagaaaaaat agttttcaaa gcaagcaaca
     1621 tccgagacag ttcgccacca gaagcgactt tcaccaaagg cttcagtggc tctcctggat
     1681 tggtcgtaat gtaaaattcg atttggtcaa atccatcatg attcaattgt ctattttctt
     1741 cttttaagaa acgtacctca aactcagtgt tttccatata cagacttttc aattcatgca
     1801 gaatggattt ttccaattct tttgcgattt ttttacgcgc ttcatgaaga tctgcggctt
     1861 gttgctgaag tagaagctct ttttgctcta gttcctcttt cattttgtcc aattgacctt
     1921 ctgaaaaatc agaagaagct agctcttcgg tgatttcgtc atagtaagcc aatattgact
     1981 caatggattc gccgtattta cgtttcaact gtcggattaa ttctaaacgt tgcgtaactt
     2041 cttctaaacg actctcatct aactccaata aatcaatctg acgactcata tcgccgattg
     2101 catcttgcag caaatagtaa gcactttgga tattttcata aattgcttca tactccacat
     2161 ctagatgggc gatcccttgc agttctgaaa cggccaagcc aacaccatct acactgtttt
     2221 gttcattatt cgtcagtgct ccgtaacctg cagctaatcc atcagcaatt ttttgataat
     2281 tgctaagttt ttctctttct tcttttaatt tctcttcttc tccgacttgc aaatcagcag
     2341 cagctatttc ttcttgctgg aaatggagca tatctattct ctgaacatag gatttttcat
     2401 tttgctgcac ttttcgtacc cttttttcca gctcacgata agagatatat tcttgctgat
     2461 aactcttttt cttctgttgg aaagctacat ctccaaaacg atccaataaa gcaagatgtg
     2521 attcaggctg gagcagttcc tgatgttcgt tttgtccttg gatatctact aaataactgc
     2581 cgattcttct cagattagct aatgtgataa tatgaccatt cactcgacaa acattttttc
     2641 cagttaagga catatcccgt cgaacaatca gattgtcctc atctgtttcg atccctaatt
     2701 caaccatcag ttcagagaat ccttcttgtt ttggtagttc aaacaaccct tcaagcacac
     2761 atttttctgc tccttgacgt atataatcgc ttgaacctct tcctcctgct aacagaccta
     2821 aagcatcgat aataatggat ttacccgcac cagtctctcc ggtcagcgcg gtcattcctt
     2881 cgtgaaaaga aagccgcaat tccggaataa tcgcaaagtt agtaatactg atttcttgta
     2941 gcatcacatt cgctcctcaa cttaaaggta atgcaggaag tctttcttca ataaaactgc
     3001 ctggtcttca gttcgtgtaa tcatcaaaac attatcgtca tcattgataa tcgaaaataa
     3061 ttctttcttg taacgtttat caatcaaatt tgctgctgcc gaagcatttc ccggcaatgt
     3121 tttcaaaata acaaattttt ccatttggtc aactgccacg aaagcgtctt ttaatagttt
     3181 ttccaattta gtatttacat cttcgtttgt ttcagctggt atgctataac ggtagccgcc
     3241 atctgaagcc ggtaccttga tcagtttcag ttcctttata tctctggaaa tcgtagcttg
     3301 agtaacagat attcctttgc ttttcaggat ttcaacaaat tcttcctgct ttcggatatc
     3361 ttgctcactc aacaatcgag taatcaaacg atgccgatct ttttttctca ttacgtcttc
     3421 gctccttagc atattcttct taatcaatga tagcttattt ccttggtata ataaagaaga
     3481 atccgcatac ttctcacttg attttcacag ttacaattta tttatggtca aactgttcgt
     3541 gtgcatttga aaccacttct gccatattaa tagctgaatt gattgttccg ctttcgggta
     3601 ctttcctcaa gtgagccaag aactcgatat ttccttctcc ccccgtgatc ggagaataac
     3661 ttaattccag cacatcatat ccaccttgtg ccgcaaatga caaaatgtct tctaaaactt
     3721 tttgatgggt ctgagggtca cgaacaattc cgtttttccc tacagcttct ctgcctgctt
     3781 cgaattgggg tttgattaaa gcaatcactt ctccctcttc ttttaaaata gcatgtaatg
     3841 gaggcagcat cagttttagt gaaataaaag aaacatcgat aacagcaact tctggaattc
     3901 ctaaggtaaa gtcttcgggt tttgcataac gaaaattcac tctttccatt acgacgactc
     3961 taggatcttg tcttattttc caagctagct gattgtagcc aacgtctaaa gcataactca
     4021 tttttgcgcc attttgcaaa gctgcatcag taaatccgcc agtggatgca ccaatatcta
     4081 acaatgtttt tccttcaaca gatagatcaa atgctttgag tgctttttct aattttaatc
     4141 cccctcgaga aacataagga agttttttac ctttgactct taattcacta ctcactggaa
     4201 ttttttctcc cggtttatca aaccgctcgt ttttttcgtt gtaaatcaaa ccagccatca
     4261 ctgaacgttt ggcttgttct cttgtttcaa ataatccttg tttgaccgct aatacatcta
     4321 cgcgttcttt ttccattctt tccctcattt ctccacatca aattgcttga tcatctggcg
     4381 catcagcccg ccatcaaaag aaaaaacgtc ctcttctaat ttgtctatga tcttgttggc
     4441 ggataacagc tcaatctcca acgcctcacg tgtcttttct aggcctaata aacgcgggta
     4501 agtatttttt tccatacgtt catctttacc agctgttttc cctaatgttt tcgttgtacc
     4561 aatcacatcg agtagatcat ctcttatctg aaaagctaaa cctaaatgtt cggctaatcg
     4621 ttgaagtagc aataagattt cttcaggttg cttcgctaat atgccaccag ccagcaaggc
     4681 ataacgaatc aaatgccctg tctttctttc atgaataaat gctaattctt ctaaagaaag
     4741 ctttttcgat tcgccctcga tatctgcagc ttgcccagca accatacctt gtgatcccgc
     4801 acaaacagct aattgctgaa gcagcagaag cttaggagaa ttacctaagt gtgccatact
     4861 gattagctgg aatgctcctg taagcaaacc atctccggcc aatatagcta atgcttcgcc
     4921 gaatacttta tgattggtag gttttcctct acgaagatca tcattgtcca tcgcaggcaa
     4981 atcatcgtgg atcaaggaat acgtatgcac catttctaaa gcagctgcta cttgatagac
     5041 gggaacatca ataggctcat caaaggaagc aacggccgtt aaaagtaata agggacgtat
     5101 tcttttcccg cctgctttca ctgaataact catcgcatca tagagtcctc tatccgttgt
     5161 atgctcatct aaaaaagaca agatttcttt ttctacagca ggtagatgaa tagctgaaaa
     5221 gtcagttaat ctcatctcag ctttcctcgt ttccgtcaaa atcaacttct tcatttgatt
     5281 ctgtcatcat tttcgtcaag gtctcttctg ctttagctaa tgtatcttgg caatgtttgc
     5341 ttaattccat tcctcgttta aacgaatcta gagctgattc taatggaacg tctccttgtt
     5401 ctaactgcat gacaattttt tctaattctt gcagtgactc ttcaaaagtt ggattttcca
     5461 tatttttatt ctcctttgtc tatctcttgt acttgtcctt taacgattcc atcttgataa
     5521 tggatcgtca gcaaatcccc ttgatgaatc tcggtgacac tttttactac ttgatcattt
     5581 aacgtcgtat agctgtagcc tcttcccatg attttcaatg gactcaacaa atcaagagat
     5641 tgaacagctt gagcaaaacg ctgctgctgt tgtttcatat attgttggat tttctcttct
     5701 aagcgttttt ctagataagc agtttcttgg cgagctgctt taactcgata aagcggagcc
     5761 acttgttcca attggtggct taatgcgatc gtttgttttt ctttcgcatg ataaatttgc
     5821 tgtgtagctt gatataatcg ctgtttcaac tggtctaatt tgatggtctg accttcatac
     5881 aatctttcag gctgccgaaa tacataggaa cgtcgagcac gctcgaaccg ttcttgtttg
     5941 cgctggatct ggcgtacaaa cgcctgctgc aatctagctt gccgttcttg aatgcgcatc
     6001 aactcttctg acaaaacagg aacagctaat tctgcagctg ctgttggagt ggcagcccga
     6061 acatctgcta ctaaatcagc gatcgtggta tctgtttcat gtcctacaga tgaaatcacc
     6121 ggcgtattag cttgataaat cgcccgagca acccgctctt cattaaacgg ccataaatcc
     6181 tcaatcgaac cacccccacg accgatgatc attgtatcga aattgcccat ctcttctact
     6241 ctctggatat tcctgacaac attgtccgct gcttggtttc cttgtacaac tgttgggaac
     6301 aaaaccaatt ggacaattgg atatcttctt ttcaccgtcg tgatgatatc gcggataacc
     6361 gctccgcttg gactggtaag gaccgcgatt cgctttggga aacgtggcag cattttttta
     6421 ggcccttgaa aaaggccttc cttcatcagc ttttcttttc gttcttccaa ttcttgatat
     6481 aaagcaccta ttccatcagg ctccatgtgc tctacataga tttgataaga acctcccgct
     6541 tcatataatg aaatacgccc aacgacaagg actttcattc cttcttttgg ctggaatcgt
     6601 aatttttgga atgctccttt gaacataata gcagatattt ttgcatggtc atcttttaag
     6661 ctgaaatatt gatgggcatt ggcacgtaaa cggaaattcg atatttcgcc tgttaaatat
     6721 acgcgttcta aataaggatc ggcatcaaat tttcttttca aatacttcgt taatgtagtg
     6781 acagttaaat attcttgtgc catctattca cctgctccaa aagcttcggc agattttacg
     6841 gtttgataca tcagcatcgt gatagtcatt ggccctacac cctttggcac aggagtgatc
     6901 cagcctgcaa taggctccac ttcatcaaat ttcacatcac cgatcagctt accgttagca
     6961 tcacgattca tccctacatc tatcactaca gcacctggtt ttacgaattc ttttgtgaca
     7021 aaatgtccac gtcctattgc tacgactaaa atatctgctt cttgtgccag ttcagccaga
     7081 ttttcggttt tagaatgagc aacagtaaca gttgcatctg ccatcaagag tagctgagcc
     7141 atcggtttac cgacgatatt gcttcgtccg ataactactg ctcgtttacc cgccggatcg
     7201 atagaataag cttcaaacat tttcatgatg ccgtatggag tacaaggaat cttgtccggt
     7261 ttacccgcaa ggagtctgcc taaattcatc ggatgaaaac cgtctacgtc tttttcaggg
     7321 cggatagcta acaggatctt ttcttcatct atatgctttg gcaatggtaa ttggaccaat
     7381 ataccatgaa aactaggatc ttgattgtat ttttcaattt ctgcaagtag ttcgtcttca
     7441 gaaatcgtct caggataacg ctctaccaat gaatggattc cgatttcttt tgctacacgt
     7501 tctttatttc ttacatagat ttggctggct ggattttccc ctaccaataa aacgactaat
     7561 ccgggatgga taccattatc tttcaatttt tggatttttt cagcaatctc tgcctgcatt
     7621 ttttcagcta attcttttcc gttaatcaat tctgccatct aaccactccc agatattttt
     7681 tgtgttttca ttctaccata gaattttccc gatttataac aggtttatga tgattcatag
     7741 aaaaaagcta ggacttacgc cccagcttct aattctttca atacattgga taaaacaccg
     7801 ttgacgaatt tacgtgaacg gtcatcacta aatgtttttg ccaactcgat tgcttcattt
     7861 aaagcaacag ccgctggtac atctgtcaca taagtcattt caaaaatcgc gatacgcaag
     7921 atcaccaaat ccatttttga gatccgattg atgtgccaat tgttttttaa atgtttttca
     7981 atcactttat ccaattcatc tttttttgca cagacaccgc caactagcgt atcaagataa
     8041 acaggaacaa actctgattc atcttcgttg atcatgtcgc ggtggtctaa ttcgattgcg
     8101 ttgaaaatcg catcttcttt tgttaggtct gcattgaagt ctaatggaaa taaagcctgc
     8161 aatgccattt cccgaatctc gtgtcttgat aattctttat tcattctctt cctcttcgtc
     8221 ttcaaaaagt tcttcgattg ttggttgttc taatttttca ggaacaacag ctactacatg
     8281 gatattgact tcagataaat cgatatccgt catgaaaagg acttgctggc tgacccgttc
     8341 ctgcatttcc attgctactt taggaactga aacaccatat tctagataac agtaaagatc
     8401 tactttcaag ccatcttcct cgtttgctaa gtaaacacct tttccgtgag ctgcacgtcc
     8461 taataattca gtgacattgc tggcaaaagt tccgcgcatt ccgtatacgc cttctacttt
     8521 agaagctgca atcccgataa ttacttcgat gacttcagga gcgatcacga tctctcctaa
     8581 atcttggttt gcgtttaaaa tcaaattttt ttcatctgcc atttatttat tcctcccttt
     8641 gcctaagcac gagatacatc cgaattctct atgatgcggc atagtgatca cgtggtaaga
     8701 aaactgccgc cgatcagctg caaactgcta tgaattggag aaaacaccca actttattat
     8761 ctcttacagc ttaaaagttc ttctatagtg tatcattaaa tcgagtgatt ttctataaca
     8821 aagtctgtga acactgattt ttacgcttac ttcttctatt gatgaaacta taagatgatc
     8881 aattcttttg gtgaacgagt caacacacga tttccatctt ctgtgatcaa caagtcatcc
     8941 tcaatacgta ctccaccgat tcctggtaag tagatccctg gttcatcagt gatcacattt
     9001 cctggtacaa attgcttttc tgcacgagaa gaaacgtttg gtccttcgtg gatctctaaa
     9061 ccgatcccat gtcctgtaga atgtccaaat gcttccccgt aaccatgttt tgaaatataa
     9121 tctctagcaa ctgcatctaa ttggacacca gtcattcctg gttttgcagc atcaataact
     9181 gctaattgag catctaagac tatttgataa atttctttta atttttcccc aggatcacca
     9241 atagcgaatg tacgtgtcat atcggaaacg tacccttcgt aatagcaacc aaagtccaat
     9301 gtgatcaagt caccttgttc gatgattttt ttgcttgcta caccatgcgg catagccgaa
     9361 cgcaatccgc tagcaacgat cgtatcaaaa gaaacacctg aagcgccaag agaacgcata
     9421 taaaagtcta gttgatttgc gacttcaatc tcagtcatgc ccggttggat cattttcagg
     9481 atgtgatcat atgccatatc cgcaatatga catgcttttt cgataatagc gatttcttct
     9541 tcatctttca cttctctgag ttcttcgatc atctgtgaaa caggtactaa ttccgcatcg
     9601 atgatttctt ctaaaaccga atattctaaa aatgagactg ttgtttcctc aaaagctaat
     9661 gagtgcagtt cttctttgcg tactaagttt gctacctcat caaaaatagg tccgacattt
     9721 ttaataattt caaacccttg agcctgcgct gccgcctgct cggtataacg aaaatcagta
     9781 ataaaaaatg ctttttccaa agtaataaca gctaatcctg tcgtacctgt aaagttcgtt
     9841 aagtaacgaa gattgtacgg actggtaacc aaaaatgaat ccaaattttc ttcttgcatt
     9901 tttttgcgca atttttctac acgtagcatc ataatgaaac tccctctttc tttgcacgct
     9961 tttttccatt atagcaaact tttcttcgta cacgacagtt aaataaaaat ttccggaaaa
    10021 cttcttttta ttttaaataa ataaagccaa aaaggagtca cagtctcgct gcaactcctt
    10081 ttagtttact attttgtaaa aacttatgat cgttttccaa agaaaaatga gacaactgct
    10141 accaaaatca ctgcgcctaa aatagatgga ataagtgcca tacctgcagc ttttggtccc
    10201 cattcgccga aaatcgcttg tccaatactt gaaccgacta gtccagcaat gatattcgtt
    10261 acacagccca tcgaatttcc tttactggtg atcgcacctg caattgctcc gatcacggct
    10321 ccaacaatca atgtccataa ccaactcata atataacctc cttctttgct aatcaatttt
    10381 agcatatggg atttaaaggg gacaaataat tggttttgct atttcatatt ttcttctgca
    10441 aagtaaaaaa ataaagaaaa cctcctctta gaagttttct ttatttttta cttgcgttta
    10501 aacatactac gaataccacg tttcactaca tcaaagaaac tcaatgattg taccattcta
    10561 tcttcatcta catattctct gatcgcacga agcacttttt taggttcttt ggaagaaaat
    10621 gtgtatgtac catttttttt cgtttgaatg gcatatcgag gaatccattt tccctttagc
    10681 atgactgatg ctagtacata atccacttct tcccatggaa tttggatata tttgcgtcta
    10741 tctcgcgcat ggtaaaactc aaaaccatta tcacctatca tgatttgccc gtaatctgtc
    10801 agacccgtaa acgaagtagc atcaattacc aagtctactt ttgtattgat tgattgaacc
    10861 attttctccc tccagatatt tgtttatgaa cagtatacta tataaaaaaa gctcgggaca
    10921 acgcccgagc tatattttta tggattataa aagtccgatg acgtgtccaa caacaccgac
    10981 tacgaataaa ccaaggatga taacgattgg agaaaccttt ttcttaagca accacataca
    11041 taacaatgtt aatcctaatg cagctaaacc aggaatcaat tggtctaagt tgtcttgtaa
    11101 tgttgttacc ttttcaggag aaagggctaa gccattatta acttgttcaa atgcactctt
    11161 gattccttca ccacctgaag gcaatttatc ccattcgatg taagcacctt tatctaattt
    11221 tacagttgat acaactggta agaatttgat tgatacccat ctttctacaa gggcagctaa
    11281 tacgaacatc cctaaaattg aagcaccttt tgttacgtct tgtaacaatc cgccagaaag
    11341 gtcatcagta attttagaac cagctttgta gccaaattct tgtgtatacc acatgaatgc
    11401 ccaacgaata atgttccaac ctaagaagaa gatgattggt ccaaggatat ttcctcccat
    11461 agctaatgaa gcacctagtg cacctagcat tggacgtact gtaaaccaga acactgggtc
    11521 accaacacca gctaaaggac ccatcatacc tactttaacc ccttgaatcg ctacatcatc
    11581 aacaggcgcg ccattcgcac gttcttcttc cagagccaat gtaaccccta aaattggaga
    11641 agcaatgtat gggtgtgtat taaagaattc caagtgacgt tttaatgctg cagaacgttc
    11701 ttcttttgtt ttgtataatt ttttgattgc tggaatcata gaaaatgccc agccaccgtt
    11761 ttgcatacgt tcgtagttcc atgaaccttg aatgaaggta gagcgccacg caacagctaa
    11821 acgatctttt tttgataatt tgattttttc ttctgccatt ttattttcct ccttcaagat
    11881 taatagtcat tcaaaatgtc gcctaaaggg tcaccagagt tgcttccgcc tccattattt
    11941 gatgaaccac ccattttaga aaggttcaag taaataagag ctaatgcaac acctaaagca
    12001 cctagagcaa tcaatgttaa ttgagaaatt gcagctacaa cgaaaccaat aacgaagaat
    12061 ggccatactt cttttgtagc catcatgttg attaccaaag cgtaacctac agctacgacc
    12121 attcccccac cgatagccat accatctgtt aaccatgctg gcattgattc aaggaatgat
    12181 tgcacagttt gtgctgggat aaatagcaac gctgctgctg ggaccgcgat acgaacccct
    12241 tgtaaacata cagcaatgat gtgccacatt tcaaccttac ggatgtttcc ttcttcagca
    12301 gccgcatcca tcaaatgaac gattggaaca gcgattgtac gtacgatcat tgttaagaac
    12361 aaccctgcaa cagccaaagg aacggcaatc gcgatcgcag aaggaacacc tttaacacct
    12421 tgtccaccta aaactaaaat aattgctgat gctactgatg ctaatgcagc atctggtgct
    12481 acagcggcac cgatatttgc ccaacctaaa gcgatcatct gaagcgtacc gccaagtacg
    12541 ataccagctt cgaggttacc ggtaactaaa ccgatcaatg tacatgccac tagtggttga
    12601 tgaaattgaa attcatcaag aataccttcc ataccagcta gaaaggcaac taatacgact
    12661 aaaattattg aaataataga catgataagc ctcctataaa ttttaatatt atttattacg
    12721 cttttgctaa ttcagctttt gcttttttca aaatatcatc catgttatcg cgtgaatcat
    12781 taggtacttt acgtacatcg aatttcacac ctttttgttc aagcttctca aacgcttcaa
    12841 cgtcttcttg tcccatagac aatactttac ttacaacgac tttaccgacc gaatgcgcca
    12901 ttgacccaac atttaattct ttgatgtcta cgccaccttc aacagctgtc aatacgtctt
    12961 caggattttc aaataaaagc aatgctttgg tatttccaaa acgagggtct tttgctactt
    13021 cgatcatttt gctaatagga ataacatttg cttttacacc tggaggagca gcttgttcga
    13081 ttagtctctt acgcagatca tcctttgata ctgcatctga aacaacgata atacggtttg
    13141 gttgcactgc ttttgtccaa gctgtcgcaa cttgtccatg taacagacgt gagtcaacac
    13201 gtgctaatac atatttgatt tttccgtctc cgaccacagt tccttctgga agtgctcctt
    13261 taggttgtgc gtcttcaact gctgctttcg gtgcttctgc cggttctaat tcttccggtt
    13321 ttacttttac accgtctttt gctgtttcaa tgatgtgtgc tgcaatttca tgagcagatt
    13381 ccattgaaaa gcgtgatgcg taggcttcaa tcaacattgg taaattcaaa ccgctgacga
    13441 ttgcccattt atctttgtgc tcttcgaata atgtgtttga ttgattgaac ggtgttccgc
    13501 cccataaatc aactaagaat aacacttcgt cttgattatc aaaagaagca accgcttctt
    13561 ccaatttggc tctcaagtca tctggacctt cacttggttt caaaatcacg gctttgacgt
    13621 tttcttgttc gccaaagatc attgaaccgg attgtaagat tccttcagca aattgtccat
    13681 gacttgctag gataatccct accatattta tacctcctat ttacttttgt tgtaacaaat
    13741 tcaaaaatat taataattgc taaccattac aacagaatta tttgaccttt tccaacaacc
    13801 attcttgcta cacctttttg attttcgcta gtagttccat caaaaggatt ttcctgtcag
    13861 caggtacttt tcgaatctct aactccactt cttgactgtt caagtaacga aaggaatcaa
    13921 tatcttgttg atttactgaa acaaaattgg taatcattga tttgccttca gtaaaactca
    13981 taccgccgat attcaacgac ttcaactgta caccgcagcg gacaaccgct tcaacatctt
    14041 ggggattggt gaataaaagc atcacttttg ctcgtgataa taatccacta tgatagattt
    14101 gaatcatttt ctgcacggtt attacatttg ctgtgatccc cggcggtgca gcttctttta
    14161 gtagaaactt tctcaattta tcttgagcga ctacatcgct gatcacaatg atacgttcaa
    14221 cacctgtttg cttcgaccaa actgttgcga cttgcccgtg gattaaacga tcatctatac
    14281 gtgccaatcg aatatccatc tgcaaaactc cttcttcctc actacttcac agaaacccct
    14341 atcatttctg tgtgaacaac cacctcctag cacacactac tataagcaaa aagcgtgcca
    14401 acttttttac gatacactaa ttgaatttaa aggattctta accaatacac aaaaacgata
    14461 cactataaaa gtgtatcgtt tttgtgtatt catggcacag tgtattcttt tttatctaac
    14521 tgattatgga ctagacgtag cagataatac atttcttcta tcggcacttt tacttttatc
    14581 tgatcttcca acagaacaac tgaggctgcc aattggctat aaagaggatc attcgttgta
    14641 tttgtcaatt cgttttcttc cacagtcaat ggttcattcc gaataatacg ttcgatcatt
    14701 cctgctgtgt gcatcactag gttgattttg aatccatatt cttcatcgcc gattcccgtc
    14761 gtctgagaga tttgtgtaga aaattgccac attggatcga ttaacttact accattaata
    14821 aagatgaagt tatcagagat aaactcaaca cacgtatttt tagctgactc ttcatctagg
    14881 gagatgaatt cagtttcctc caattctgat tccatcagca actgatccaa aatgacttcg
    14941 atattttgat caataaaacg ttctaaagga ataaatgggg cttggatatt caaatcagta
    15001 atcccagtag tagctatgat ctgatattct ttttggataa ctggcagttc ttctttcact
    15061 tccacaatcg acaaagctag aacttctaat tctgtttctt gacgcttgtt taccgctcgt
    15121 tcgatcaatt ctttgattct ttgtgctgta ccttcgccag atgcgcagat agccaaaaca
    15181 gcttttttct tatgaatagg ttttgcttcc tgaacagatt ctacagaaga tatttctgcg
    15241 taacctcgaa aatttttcaa tgattcatgc aacatatcta gatctgtccc taatactgag
    15301 acctttcgta cagtttccaa aactagagaa gttgttacca tatcaacagt acgtactgga
    15361 attccagtgt ctctttgaat ttgcgaatta aacgacgcaa gggaccccat atctactaag
    15421 agtatgaccc cgcttccttc atctacttct tgtacattgc gttcaattcg gtccaatgct
    15481 gttttcggat ccatatccaa aggcatgtct actgctcgta cgttctctgc atctaacaac
    15541 tgttggacaa cttggaccat actgcttgct gtgctgtttc cgtgagctgc aacgactaca
    15601 ccgattctgc cgcttgcttg atttgcccgt aacgagacga gtagaacggt caagtaatag
    15661 tcttcacttt ctggaatctc cacttgaaaa tacgtttcga tataactacg cacttctttc
    15721 gcaacagcat attcatctgg aaaatcaaaa gccatctcac ggatgttatc attcgtatgc
    15781 cgttcttctc ctaagttgat ttttttcaaa aaagaactga tatgcaagct catagcataa
    15841 ataaagtttt gctgaaactc atatggtaaa gatcttttga ccattacata aatctgattg
    15901 gtcacttcta tgattttcgg atctacaaat tctgctaatt tgctgtctgc attaaaagaa
    15961 aaaccatggt ctttatagaa agacttcaga tggatgttga tatctgtaga aatgaaatga
    16021 ttgattgctt cttgatccaa accatccgac ttcagtaaag ctgctttatc tccgatgata
    16081 tcatatagat tataaggtaa ctcataggag tccgattgga ttttcatgat cgtatcattt
    16141 ggtgctacag ttatctttgg ctccaatatt ttagaaagct ctgccattgc actgcggttg
    16201 cttgctaatt gaatcaatcc gctacgaatc ccttctgtca aatcatcgat tgtgatcgag
    16261 atttcgggtg attgcatatg atttagaaat cctcgtgcac atactaactg gatatttgac
    16321 ttcaattgtc ctatatttcc ataggtcaca ctgccgatca atgcttttgc tacatcttct
    16381 gtcaaagaaa tctttcgctg cgtgcggttt gcttcgtggg caatcatgat ttttactaaa
    16441 tcgactttct catttgcagg acgtttttca aaagccggaa gtttgatatt gattgggata
    16501 cgccgaacaa aagtttctaa tagagaagaa cctgggtctt ctgtggtcgc acctacgatc
    16561 cgtacatttg cttcatggga tttcgttgtt tccccaagac gactgtaagt tccatgatcc
    16621 ataaagtaaa agatcatttc ttgaccttca ggcggcaacc ggtgaatctc atccaaaaac
    16681 agcataccgc catccgcttg atcgataatc cccatttttt cttcatctgc gcctgtaaaa
    16741 gcaccttttg cgtacccaaa taaatgactc atcaaaagct cagggttatt ggcataatca
    16801 gcacaattga aaacaatcag ttccgtttcc tcatctatta catgatgcgc ttttgcaaaa
    16861 tgatacatcg cgtgtgcaaa ataagtcttt cctgatccgg taggtcctgt aatcaaacaa
    16921 tttaatcctc ttggcggata caaaatcgca gcttttgcct gttccactga atttttcatg
    16981 ctgccgttag ctccaatgat ttttgcaaag atatccttag tatctatttg gagtgtattt
    17041 ttttcttcag agaccggcgt ttctttgtaa ctgactacat gttttgtaac tggctttacg
    17101 tgcgtaacga gtgtttgata tatgtaacgt actggtcgtc cgtctgtctt tatcagtttt
    17161 ccttcgcgca ctagctgatt cagatcttta ctgctatttg ttcgctgaat ccctaacact
    17221 tcagcaattt cctgtgttgt tactccttga tcatattctg cctttgttaa atgagcggtt
    17281 ttttctttga catacatata aattcggtca atgcgttttg ccaatggtat cgctcctttt
    17341 ctatagacta ttgtacttag gcttcaaaac ataatactat gtatttattt ctgtttcgtc
    17401 aactatttac ctgattttgt tttgttgata acacgcggat agatagaaat gttcaattta
    17461 aaagaacgaa aaatcccgat acgattttta taatggaaac aagaaaaaga acactgttta
    17521 ccatataatt atttcactga tagcaaacaa tatagcttcc atcaattgaa aacgatagtt
    17581 atcaaaacat cctgattcca aattggcaaa aaaatattaa gtgtaaaaag acaaagtaaa
    17641 cttcgtagcc tccactacaa cttttatttt tataaacaaa aaaggctgtg acaaaagtct
    17701 tgtcacaacc tctttaatca taagattcaa ttaagcttct tgagctactg gataaacaga
    17761 cacttcaatt atactgtttc ctgataagct ctcaattctt ctgaacccat gatgttagtg
    17821 cttttcttct attattaaat atcttggaat tttctattct ttctaaaaag tatggtcaaa
    17881 gtatggtcac tattgataga atgttaatag tagtagaaag ctgatatcta ggaggaacta
    17941 atgtgagtaa acaatgtgca aagtgcggag gtaaaattgg cttaacttca tataaaatca
    18001 aagataaaca aattgtttgc ggcaattgta tgaaaaaagc tggttacggt atgactactc
    18061 cttataaaat aataagatct ttgaatttag aagatttaga taaggatcca tcagaaaaaa
    18121 gaaaattaaa catatctgat catactgcac aaaacaatca tgatataggt aatgatagaa
    18181 tgagaaccac tgaagagatg gtagaatttt gtcttaaata cggctacggt aaaggaatga
    18241 ctaaaaaatg gacaactcat cattttaact taatatcaga tcaacttaac agtgacgaat
    18301 acgtaatctt ctgctttgta ggattgcata attatatctc atcaactaag catgacaata
    18361 attatgcata cgcattaact aataaacgtt taattgtagc tcaacaaaaa atgataggca
    18421 ataatgtaca aagtattata ttagacaatt taaataatat tagtaaaaaa agaggagtgc
    18481 tgttgggtac attaactatt gatactcttg gtaaagtatt gaatgttgca gttgataaag
    18541 atactgttga t
//
LOCUS       DAFGFZ010000054        18418 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000054 DAFGFZ010000000
VERSION     DAFGFZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.054"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..795)
                     /locus_tag="LR726_001613"
     CDS             complement(<1..795)
                     /locus_tag="LR726_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL6216709.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRI"
     gene            complement(768..1448)
                     /locus_tag="LR726_001614"
     CDS             complement(768..1448)
                     /locus_tag="LR726_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL6216710.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1471..2409)
                     /locus_tag="LR726_001615"
     CDS             complement(1471..2409)
                     /locus_tag="LR726_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL6216711.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2654..3517)
                     /locus_tag="LR726_001616"
     CDS             complement(2654..3517)
                     /locus_tag="LR726_001616"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL6216712.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3659..6232)
                     /gene="mprF"
                     /locus_tag="LR726_001617"
     CDS             complement(3659..6232)
                     /gene="mprF"
                     /locus_tag="LR726_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL6216713.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6373..7179
                     /gene="proC"
                     /locus_tag="LR726_001618"
     CDS             6373..7179
                     /gene="proC"
                     /locus_tag="LR726_001618"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL6216714.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7258..8814)
                     /gene="rny"
                     /locus_tag="LR726_001619"
     CDS             complement(7258..8814)
                     /gene="rny"
                     /locus_tag="LR726_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL6216715.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9225..10274)
                     /gene="recA"
                     /locus_tag="LR726_001620"
     CDS             complement(9225..10274)
                     /gene="recA"
                     /locus_tag="LR726_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL6216716.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10383..11624)
                     /locus_tag="LR726_001621"
     CDS             complement(10383..11624)
                     /locus_tag="LR726_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL6216717.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11864..12442)
                     /gene="pgsA"
                     /locus_tag="LR726_001622"
     CDS             complement(11864..12442)
                     /gene="pgsA"
                     /locus_tag="LR726_001622"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL6216718.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12651..13574)
                     /locus_tag="LR726_001623"
     CDS             complement(12651..13574)
                     /locus_tag="LR726_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6216719.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14313..15599)
                     /locus_tag="LR726_001624"
     CDS             complement(14313..15599)
                     /locus_tag="LR726_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL6216720.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15592..16860)
                     /locus_tag="LR726_001625"
     CDS             complement(15592..16860)
                     /locus_tag="LR726_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL6216721.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17112..17573
                     /gene="mscL"
                     /locus_tag="LR726_001626"
     CDS             17112..17573
                     /gene="mscL"
                     /locus_tag="LR726_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL6216722.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17632..18078)
                     /locus_tag="LR726_001627"
     CDS             complement(17632..18078)
                     /locus_tag="LR726_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6216723.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18171..18416)
                     /locus_tag="LR726_001628"
     CDS             complement(18171..18416)
                     /locus_tag="LR726_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL6216724.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5254 a         4031 c         3226 g         5907 t
ORIGIN      
        1 tattcgtttg atgatattta caatcaggaa caaagcaata aagactaatg tgattgttgc
       61 tcctggaggt gtatctagat aaaacgagct agttagtcct gacagcatcc ctagaaatcc
      121 tacacaaaca ctgatagcaa tcacgacatt gaagcttttg cctaatcgca taccgattgc
      181 tgcgggaaga accatgattg cggatatcaa aagtgcaccg gcaatcggga tcatcacagc
      241 aatagccaca ccagtaatca cattgaacat catcgacatc aaatgaacgg gtagcccatc
      301 cacgtgggcg gtatcttcat caaaagtcag aacatacatc ggccgtttga ataaaaagaa
      361 caatataccg atggcaacga ataagattcc taaaaagatg acttgctccc atgtaatggt
      421 cacgatcgaa ccaaacagat aggattggat acttgttgct gaattccctc cgctcaaatt
      481 catcaatact aacgctagcg ccaaaccacc agccattaaa atggcaatgg agatttctga
      541 ataggttcgg tagatcgttc gcagatactc caatatcaca gctgctaata caacgactag
      601 catggtcgta agtgtaggat taaggttcaa aaagaatcct aacgctaccc ctgctaaaga
      661 gacatgagaa agcgtatcag ccatcagcga ttgtcttctg atcactaaaa acacacctag
      721 catcggggcg attccggcaa tgaagatagc ggcaagaaac gctcttctca tgaattcata
      781 tgaaagcaac gccattgaga atcctccttt ctcaccagtc ggatctgtct gtccgcatac
      841 tgtttgatat cttcatgatc atgggtgatc attagaactg atttcccatg gacatgcgca
      901 ctatgtcgga gaagacgata aaattcattt ctggattctt catccatacc tgttgttggt
      961 tcatcaagaa caaataagtc agggtcagta gcaaaaatac gcgctaaact gattcgctgc
     1021 ttttgtccgc cagacaattc acctattcgc cgatttcgca tatcccacat ccctacagct
     1081 tctaaagcac gctgtacatg ttgatgatct ttttcagaga gacgcttgaa ccagcggcct
     1141 cttggatatc ttcctgaacg gactaattcg atcaccgtac taggaaaacc tgcattgaaa
     1201 gatgcaactt gttgaggaat ataaccgata cttaattttt ttccttcttg atttttctca
     1261 gcaatcttta tcattccttt tgaaggtttc agtaatccta acgatgcttt caccaaggtt
     1321 gacttggctg ctccattttc tccagttaaa atgacaaatt cacccgcatc tacatgataa
     1381 gagacatctt ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga tatttcgata
     1441 taacgcacct tacaacctcc attctaaaac ttaattgata ctttctttta aagctttcag
     1501 atttttttcc atgacagaaa tataattttc gcctgctttg atttgttcat tagtcaagct
     1561 ttctaaagga ttcaacactt ctagcttcac tcctgtttca ttcgacaatg tctcagctac
     1621 cttcgaagag gcattttctt caaaataaat cactttgatg ttgtttttct tcacaaattc
     1681 ttttagctca gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc
     1741 tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa
     1801 tgtgcggttt gttgcatctt ttagctcatc ggagtatttt tgatccagtg cttcaagctt
     1861 tttgatataa gcatcggcat tcttcgtaaa agtttccttt ttatctggat atttttcgcc
     1921 taattcttca gcaatcgttc tgacttcttt tatagctaat accggatcga gccaaacatg
     1981 tggatcgtat tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc
     2041 ttgcgattct aaacggtcga ttcctttagc tgcttcgatc atcaaggtct gtttgctatc
     2101 cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct gaactattat acacgaaaac
     2161 atctgcatcg gaaatttctg ctctttcttt tgcacttggt tcgaaatcat gcggctccgt
     2221 tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc
     2281 atacatcgga taaaaagtcg taacgatcgt caaatcctct tttttgtctg cttccttgtt
     2341 cgtatttcca catgctgcaa acagtaaagc acctatcatt accgtagctg tcaaaagata
     2401 ttttttcatt tatatgctcc tttcaatgaa caaatcggaa ttttacgatt tattaacgtg
     2461 aactaatgta tcaaaataaa aaaacacagt caatagaaat ccatcaattt ctgatttaag
     2521 catgtatatt cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc tagccatttc
     2581 actacaataa aaaactgaag aataccattt taggattcct cagtttttca tcacctattt
     2641 tacttcttat attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta
     2701 acccattata tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc
     2761 cgcttccact catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat
     2821 ggatgactgg atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca
     2881 tctgctgata atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga
     2941 tggcttgcgt atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga
     3001 ccaacacaac ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac
     3061 cgacaaatgc cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga
     3121 tttctgccag ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag
     3181 cagctgcaca gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa
     3241 caaagatctt gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat
     3301 aaacattgtt ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt
     3361 cttctgttat ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt
     3421 ccaactcatg atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg
     3481 ctggcgcttt ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta
     3541 ttctatcata gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat
     3601 cgtttcaaca gctttcacag aattttctct tcataatcca aacaagttct agtgattctt
     3661 ataattctgt gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca
     3721 gactgagtag ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac
     3781 gtccttccca atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga
     3841 aaaaattacc ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg
     3901 tttctccaac attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct
     3961 cgtatacaat aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta
     4021 agacccgcaa caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc
     4081 caacgacttt ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat
     4141 caaatcgtcc cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt
     4201 catttacagc ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt
     4261 ctttcatcaa acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta
     4321 gatcaacaat ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca
     4381 ctacgtagct ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt
     4441 tcataaactg attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa
     4501 caaagcattt gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt
     4561 ggtaataaaa ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt
     4621 atgtttccaa taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat
     4681 cttcagctaa atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa
     4741 gccctgagaa ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac
     4801 cgactaaaaa gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga
     4861 acaagctagc atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt
     4921 cttttctgga taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag
     4981 ataacgtacg tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa
     5041 atgccttttt gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca
     5101 atagattcac tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc
     5161 gactgattgc agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat
     5221 agacagcact tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat
     5281 ctaaaaatcg attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa
     5341 aaggtagaac ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc
     5401 gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg
     5461 ggaccatcgt cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca
     5521 tcgaaatatc tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc
     5581 attgtccaaa ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca
     5641 atgtctttct ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga
     5701 aaagccagat ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa
     5761 atgtaagaaa cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag
     5821 tggctaaaac catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc
     5881 ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa
     5941 gaaactcttt tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc
     6001 aatcatacaa caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg
     6061 tcatgcggct ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa
     6121 tatttgcgac ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa
     6181 tcagactatg ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc
     6241 attcctaact tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt
     6301 ccactttaga cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa
     6361 ggagaggata acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt
     6421 gatggtttat taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat
     6481 tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag
     6541 gaagttgcat cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa
     6601 gtattggaag aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga
     6661 aaatcattaa cagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg
     6721 atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct
     6781 gccacggagg aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat
     6841 cctttagcag aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc
     6901 gtttacatgc tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag
     6961 acagccactg agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt
     7021 gataaaaatc catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca
     7081 ggtgtagttg ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg
     7141 acgatcgaga aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg
     7201 ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta
     7261 tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata
     7321 atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc
     7381 cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact
     7441 tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta
     7501 gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc
     7561 tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt
     7621 gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga
     7681 tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc
     7741 taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac
     7801 cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt
     7861 gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc
     7921 gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg
     7981 atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat
     8041 tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc
     8101 agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat
     8161 ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca
     8221 gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg
     8281 ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt
     8341 catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg
     8401 ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga
     8461 actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt
     8521 acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt
     8581 actttctctc agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc
     8641 taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc
     8701 agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc
     8761 taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc
     8821 cacctccata ctatcttttt catttttatc atctatacag cttctatgat aaattattct
     8881 aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct
     8941 aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc
     9001 attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg
     9061 agaaactttt ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc
     9121 actcgtataa ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga
     9181 gagtacctac tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa
     9241 ggcaattctt cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa
     9301 gctgctctaa ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt
     9361 ttagcgtttt cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt
     9421 ttatcaacaa tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct
     9481 aaaccataca tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg
     9541 actttgatct ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga
     9601 cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct
     9661 ggattcccaa acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt
     9721 tttgttttgt tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt
     9781 aacccgacat gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca
     9841 actgaatcga ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct
     9901 agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat
     9961 ttttgtgcat attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca
    10021 tttttttgta cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt
    10081 ccatatactt cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac
    10141 gcaagagagc cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata
    10201 atagagcctt taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta
    10261 cgatcatctg ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta
    10321 tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt
    10381 tatcattttc ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat
    10441 ctgatagcag cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat
    10501 gaaatagcga tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact
    10561 ccggtgaaag ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc
    10621 tccacacatt ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga
    10681 aaaccagctt ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca
    10741 ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt
    10801 acagtctgct tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg
    10861 ccgtatccat aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga
    10921 gcttgattcc ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt
    10981 ggtttggcat atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca
    11041 gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct
    11101 tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac
    11161 tcattaggcg gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct
    11221 aaagcaagcc ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc
    11281 agattgtttt tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat
    11341 ccttccgtat tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg
    11401 tcatcttctg tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct
    11461 agagcaatca actcttctaa acgctgctga ttatctccta caactgtttg ataataaact
    11521 tcgataccta gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt
    11581 cctagtaaaa gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt
    11641 atatagtata cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac
    11701 aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt
    11761 cataagtcac aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact
    11821 cagagttaga cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga
    11881 acacaccgct gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag
    11941 cataaagcat gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa
    12001 acagcaagat aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag
    12061 ccatgacttc tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac
    12121 agacaatgat cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag
    12181 cagtcataac tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa
    12241 ggcctcgcgc acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa
    12301 taactgcacc tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc
    12361 aatctaaagg taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag
    12421 ttaatttatt tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc
    12481 agcgaagttt ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt
    12541 tattgaaatt gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc
    12601 cttctttttt atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg
    12661 tattgaagag taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt
    12721 acggattctc cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca
    12781 tttgttgctg tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga
    12841 taaacatagg cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca
    12901 agggttacag gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct
    12961 cctgtagcta ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa
    13021 gactctttgg tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact
    13081 ttaagcgaag aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata
    13141 tagcccacga caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt
    13201 gtccaaaagc gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact
    13261 gtttcaggct gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca
    13321 tcgactaaat ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc
    13381 acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt
    13441 ttttgaattt ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct
    13501 tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg
    13561 attctccgaa gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag
    13621 tcatgtcaca gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca
    13681 agtcacaata tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga
    13741 gttaaacggc aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta
    13801 ttagctcaaa aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca
    13861 ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    13921 gtaataagtc caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt
    13981 acttgatgca gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc
    14041 ttcggcaata agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt
    14101 atttcttgaa gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt
    14161 taatataagt tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac
    14221 acaactaaga ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg
    14281 aaatttaaga cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg
    14341 aaggcctctt cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa
    14401 tcgatgattt caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga
    14461 ttggcaatat attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc
    14521 agcaaatcga gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct
    14581 ttgacttttt ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga
    14641 aattcacgat ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac
    14701 atcgctagat agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct
    14761 gttttatagc gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca
    14821 agtacaaatt tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt
    14881 gtgttttcag ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg
    14941 ttttcccgaa taagtttcat caatttctca ggctccatct taccaactac aaataaaacc
    15001 atattgcttg gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg
    15061 tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta
    15121 tttagtatgc caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga
    15181 ccgatgattc ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa
    15241 tcgatcagag tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt
    15301 ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca
    15361 tcaccatctt ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact
    15421 ttcacctttt ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta
    15481 ctgaacaaac catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg
    15541 tttggcagta cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt
    15601 tgcatttcct cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg
    15661 tatctctttg atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt
    15721 ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga
    15781 aagcagatat tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc
    15841 accatttcgc aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt
    15901 ttcatcgata ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc
    15961 ataataagct agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc
    16021 gccaaataaa ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt
    16081 tgtctgataa gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt
    16141 cacgatattc acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc
    16201 aaatggtaat tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa
    16261 aatgtcgatt tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt
    16321 gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc
    16381 aaaataaagt tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc
    16441 caaatataga cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt
    16501 tttaatattt ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt
    16561 tggatcattg acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt
    16621 tccttttttt ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact
    16681 taactttgtc tgatccgaat aattcaaact atttgtttcc aacatgctgc tgagaagtga
    16741 acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt
    16801 tttgtattgt tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat
    16861 atttatatgc tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc
    16921 ttttttactt attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa
    16981 atgatgttac actttatagt atagcataga tgttttttat caaaagaaac agacggctaa
    17041 aagcgtatat tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg
    17101 gagggtcatt catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc
    17161 ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag
    17221 gcttgatcac accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag
    17281 aaggagcact ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta
    17341 acgttattag tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca
    17401 aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa
    17461 ctcctactgc agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag
    17521 aagacaatac acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa
    17581 tctaaaaaaa agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc
    17641 tacttgttcc tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat
    17701 tgccttgaat ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat
    17761 tggtttttct ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc
    17821 taaaccatct ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc
    17881 ttcttcatat actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg
    17941 ccaagcttct ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa
    18001 aactgcgatt cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt
    18061 ctttacctga tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt
    18121 cgcacacaaa aaagcaaacg aaccttacga atctcaggct cgtttgcttt ttaaactgct
    18181 tcagttaaaa attcgatctc aactgtccga gtaagtacat ctgaatagct gtaagatact
    18241 cgttcaaaag aattttcttc aggatccaaa tcaacaacga atactgaagg ataggtttct
    18301 gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac caacatgatt
    18361 gggctgccga tacgaccttc taaatctttc ttgattgaag ctaaagttgt tggcatac
//
LOCUS       DAFGFZ010000055        18399 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000055 DAFGFZ010000000
VERSION     DAFGFZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.055"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            171..515
                     /locus_tag="LR726_001629"
     CDS             171..515
                     /locus_tag="LR726_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL6216725.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="LR726_001630"
                     /pseudo
     CDS             516..935
                     /locus_tag="LR726_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="LR726_001631"
     CDS             complement(1027..1377)
                     /locus_tag="LR726_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216726.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="LR726_001632"
     CDS             complement(1343..2674)
                     /locus_tag="LR726_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL6216727.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="LR726_001633"
     CDS             2930..3496
                     /locus_tag="LR726_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6216728.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="LR726_001634"
     tRNA            3641..3728
                     /locus_tag="LR726_001634"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /gene="bsh"
                     /locus_tag="LR726_001635"
     CDS             4062..5036
                     /gene="bsh"
                     /locus_tag="LR726_001635"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL6216729.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="LR726_001636"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="LR726_001636"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL6216730.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="LR726_001637"
     CDS             complement(7451..7897)
                     /locus_tag="LR726_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL6216731.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="LR726_001638"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="LR726_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL6216732.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="LR726_001639"
     CDS             8362..8880
                     /locus_tag="LR726_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL6216733.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="LR726_001640"
     CDS             complement(8957..9913)
                     /locus_tag="LR726_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6216734.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="LR726_001641"
     CDS             10079..10885
                     /locus_tag="LR726_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216735.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="LR726_001642"
     CDS             10882..11871
                     /locus_tag="LR726_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL6216736.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="LR726_001643"
     CDS             11868..12872
                     /locus_tag="LR726_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL6216737.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="LR726_001644"
     CDS             13004..13546
                     /locus_tag="LR726_001644"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL6216738.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="LR726_001645"
     CDS             13566..14264
                     /locus_tag="LR726_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL6216739.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="LR726_001646"
     CDS             14285..14497
                     /locus_tag="LR726_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL6216740.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="LR726_001647"
     CDS             14497..15459
                     /locus_tag="LR726_001647"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL6216741.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="LR726_001648"
     CDS             15477..15872
                     /locus_tag="LR726_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL6216742.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="LR726_001649"
     CDS             16035..16400
                     /locus_tag="LR726_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL6216743.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="LR726_001650"
     CDS             16397..18208
                     /locus_tag="LR726_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL6216744.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..>18399)
                     /locus_tag="LR726_001651"
     CDS             complement(18270..>18399)
                     /locus_tag="LR726_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL6216745.1"
                     /translation="ATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
BASE COUNT         5910 a         3113 c         3809 g         5567 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata gtagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaaagat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gaggggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaatcga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaatga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcc
//
LOCUS       DAFGFZ010000056        18304 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000056 DAFGFZ010000000
VERSION     DAFGFZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.056"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            11..1672
                     /locus_tag="LR726_001652"
     CDS             11..1672
                     /locus_tag="LR726_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6216746.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1742..2089)
                     /locus_tag="LR726_001653"
     CDS             complement(1742..2089)
                     /locus_tag="LR726_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL6216747.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2173..3114
                     /locus_tag="LR726_001654"
     CDS             2173..3114
                     /locus_tag="LR726_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216748.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3115..4158
                     /locus_tag="LR726_001655"
     CDS             3115..4158
                     /locus_tag="LR726_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6216749.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4164..5234
                     /locus_tag="LR726_001656"
     CDS             4164..5234
                     /locus_tag="LR726_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216750.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5224..6177
                     /locus_tag="LR726_001657"
     CDS             5224..6177
                     /locus_tag="LR726_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6216751.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6189..7199
                     /locus_tag="LR726_001658"
     CDS             6189..7199
                     /locus_tag="LR726_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6216752.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7484..8305)
                     /locus_tag="LR726_001659"
     CDS             complement(7484..8305)
                     /locus_tag="LR726_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL6216753.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8402..8680)
                     /locus_tag="LR726_001660"
     CDS             complement(8402..8680)
                     /locus_tag="LR726_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216754.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8948..10957
                     /gene="metG"
                     /locus_tag="LR726_001661"
     CDS             8948..10957
                     /gene="metG"
                     /locus_tag="LR726_001661"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL6216755.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11436..12023)
                     /locus_tag="LR726_001662"
     CDS             complement(11436..12023)
                     /locus_tag="LR726_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216756.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12198..13520
                     /locus_tag="LR726_001663"
     CDS             12198..13520
                     /locus_tag="LR726_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6216757.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13675..14451
                     /locus_tag="LR726_001664"
     CDS             13675..14451
                     /locus_tag="LR726_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL6216758.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14444..15013
                     /gene="rnmV"
                     /locus_tag="LR726_001665"
     CDS             14444..15013
                     /gene="rnmV"
                     /locus_tag="LR726_001665"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL6216759.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15006..15890
                     /gene="rsmA"
                     /locus_tag="LR726_001666"
     CDS             15006..15890
                     /gene="rsmA"
                     /locus_tag="LR726_001666"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL6216760.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16341..16970
                     /locus_tag="LR726_001667"
     CDS             16341..16970
                     /locus_tag="LR726_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216761.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17094..18203)
                     /gene="ugpC"
                     /locus_tag="LR726_001668"
     CDS             complement(17094..18203)
                     /gene="ugpC"
                     /locus_tag="LR726_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL6216762.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5967 a         3307 c         3745 g         5285 t
ORIGIN      
        1 ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact
       61 tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc
      121 agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc
      181 agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg
      241 tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga
      301 agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc
      361 tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc
      421 tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa
      481 agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac
      541 gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca
      601 atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa
      661 tggaccattc acattagaag attttgatgg accaggaaca gatacagaat ggacttataa
      721 gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt
      781 agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat
      841 cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga
      901 agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt
      961 gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact
     1021 aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac
     1081 aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc
     1141 aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt
     1201 gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca
     1261 agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga
     1321 ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga
     1381 cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag
     1441 caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca
     1501 agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt
     1561 tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca
     1621 ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat
     1681 accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca
     1741 cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa
     1801 tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt
     1861 ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac
     1921 ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata
     1981 gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa
     2041 ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc
     2101 tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag
     2161 ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc
     2221 atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg
     2281 ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc
     2341 ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga
     2401 gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga
     2461 atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg
     2521 attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc
     2581 gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc
     2641 tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc
     2701 ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg
     2761 gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc
     2821 cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta
     2881 ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct
     2941 attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc
     3001 atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta
     3061 ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa
     3121 atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta
     3181 caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa
     3241 gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg
     3301 gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa
     3361 aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat
     3421 ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca
     3481 aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt
     3541 atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt
     3601 ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa
     3661 cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc
     3721 gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca
     3781 atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct
     3841 ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc
     3901 tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc
     3961 tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg
     4021 agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact
     4081 ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat
     4141 cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct
     4201 cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata
     4261 aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa
     4321 gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag
     4381 gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag
     4441 cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag
     4501 tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat
     4561 tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc
     4621 atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc
     4681 cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga
     4741 ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc
     4801 atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa
     4861 tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg
     4921 gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg
     4981 gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat
     5041 tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca
     5101 aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc
     5161 aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg
     5221 ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa
     5281 aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc
     5341 ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg
     5401 aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat
     5461 aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct
     5521 tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga
     5581 ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga
     5641 ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt
     5701 attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt
     5761 tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa
     5821 gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa acatattagt
     5881 gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc
     5941 tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat
     6001 ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa
     6061 gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc
     6121 ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag
     6181 ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc
     6241 aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc
     6301 agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa
     6361 aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt
     6421 tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga
     6481 aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa
     6541 atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag
     6601 ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg
     6661 cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg
     6721 cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat
     6781 ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct
     6841 ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa
     6901 aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat
     6961 ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg
     7021 cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt
     7081 cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt
     7141 ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa
     7201 aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac
     7261 ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa
     7321 acaatagcag aaaaagttcg tacgagcttt ttctgctatt gttaattttt tattcagaaa
     7381 gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc
     7441 gttttttctt gaatgattag gtctgaatct tttgctcaca accttattca tggtgccccg
     7501 gcttgagctg atagattttt gaaggacggc cgattcctac aggacgttca ccgatctcat
     7561 cgaaatattg gagcatagct ttcttaaaat tggaatgatc gatcgtttta taatcgatcc
     7621 ctaagaattt agcaaatact tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt
     7681 gtagaacttg cggctcgtgt tccattttat tgactacacg gttgaatgct ttcaagatga
     7741 tttgactatg gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg actaaatcaa
     7801 gcgagatagc cacttctccg ctagataaat ggatcttgtt actatgacgt tctagtgtga
     7861 accagcgtac ttccttggca tcatctccgg cgattagcgg ttcttctcca ataaaggcta
     7921 agtagctgac tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg aatgtatgca
     7981 gttgctcaat attttctttt gaaataacca caccagtttc ttcctttgtt tctcttaaga
     8041 cgctttctcc agttgactca tccttttgga caaagccacc aggcaatgcc catgaatttc
     8101 gataaggatg cccttttctt tggatcaaca aaactttcag ctgatcttct tctttgttat
     8161 agcacatcag tacgatatcg acagtcaaag agggcttgtc atattctgga cgttcctgtt
     8221 tttgatacca gttaagaaat tcttcttcac ttgctttatg ttcataatat aatttttctt
     8281 cttctttaga agcaaaacgg ttcatctgac cacctccatt gacagtaaaa gtacttttta
     8341 ccttattgta gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc taggttatca
     8401 tttaaatatg aaaaataaat ggaagtatga gattgaataa aaagaaaagc agataaaacg
     8461 gcaatacttt ataccgctct tcacgaatgg caaagaagcc acaaataacg atatatacgt
     8521 caatgataaa acgcaaaatc caagctgctg tcataaaact gttcaaatgc ccatggaggt
     8581 taaaataacc atacatacga atataaaaat agctgaatat taaatgtgct aatgaaagaa
     8641 aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca gtcccttact
     8701 aagatagata gtctttatta taccacgtgt gaggggagaa ataaaaagcc gttttttagt
     8761 agatatgaaa caaaacaaaa acccgcattt attcaaccta ttcatccttt taaaggatag
     8821 attgaacgta gatagaagtc gtttatccag tctttttcat tttgaaaaaa agctaacagc
     8881 atgctttttt tatggtatac tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa
     8941 gagccccgtg gcagaaaagg aaacttttta tatcactacg ccaatctatt atccaagcgg
     9001 caaacttcat atcggcaact cgtatactac gattgcatgt gacgcaattg ctcgttataa
     9061 acggttgatg ggctttgatg tgttttatct cactggagtg gatgaacatg gtcaaaaaat
     9121 cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa tatgtagata aaatggcggc
     9181 tgatgtcaag aaattatgga aaacgcttga tatcagttat gataaattca ttcgcacgac
     9241 agatgattac cataaagcag ctgttcaaaa aatatttgat cgtttattag aacagggaga
     9301 tatttactta ggtgaatatg aaggctggta ctctgtttct gacgaagaat tctttacaga
     9361 aacacaactt gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc
     9421 aagtggacac gaagtagaat tggtaaaaga agaatcatac ttcttccgca tgagcaaata
     9481 cgcagaccgt ctattggaat actatgaaga acatcccgaa ttcatccagc cagaatctcg
     9541 taaaaacgaa atgatcaaca actttatcaa accaggatta gaggatcttg cagtttcacg
     9601 aacaactttt acttggggag taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg
     9661 gatcgatgca ttatccaatt atattactgc actaggatat ggctccgaag atgaaagtct
     9721 attcgaaaaa tactggcctg cagatgttca gatggtcggt aaagaaatcg ttcgctttca
     9781 tacgatctat tggccaatca tgctgatggc ccttgatttg cctctaccta aaaaagtatt
     9841 tgggcatggc tggctattga tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt
     9901 ttatcctgaa atgcttgtgg aacgttatgg tttagatgca ctccgttatt atttgcttcg
     9961 tgctgttccg tttgggtcag atggtgtctt tacgccagaa gattttgttt cccgtgtcaa
    10021 ttatgatctg gcaaatgatc tagggaactt attgaatcga acgattgcta tgatcaacaa
    10081 atattgtgat ggactcgttc ctgactatgc ctcacttgtc actccatttg atagtgaact
    10141 gtctacgacg gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt
    10201 caatacagca ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat acattgatga
    10261 aacagaacca tgggtattag caaaagacga agaaaagaaa gctgaattga atagtgtcat
    10321 gatccattta gctgaatctt tgcgtatcgc agcgatatta cttcagccaa tcatgacaga
    10381 aacgccagag aagatcttca atcaactagg attggactct gaaacaatga atcttgaagg
    10441 attacacttt ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta caccaatctt
    10501 cccacgttta gatatggaag aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac
    10561 tcaaacaaac gaagatacag tcaaatggga tccagaagaa acagaacttg tttcaacgaa
    10621 agaaaaacaa atcaaatttg atgtatttga aaaagtagaa ctaaaagtag cagaagtcat
    10681 caactgtcaa aaagtagaag gggcagataa gttgctgcaa ttccgactag atgctggaga
    10741 cagccaagac cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc caagcgaatt
    10801 gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat
    10861 cagtcaagga atgatccttt ctgcagaagc accagatggc tctttacaag taatcgaagc
    10921 accaaaatcc atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg gtatgacact
    10981 agttgtgtca tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta
    11041 ttacttgaag ccaatcatat aatacagaaa cagagaacta tccaaaataa atcctagaaa
    11101 aagaagtacg agcttcgctg atttttgtta atttagaata atactaatag gaatcgtaaa
    11161 aacagaaaaa aagcataatg ataaagggtc gggcagattt taattgattc atctggtact
    11221 cttttttatt gtccttacat tcataaagtt tgaatgcaat gactttatta catctatatt
    11281 tatatctaac agtagcgttg atgtgttaat tataagttct atcactttat gttcaattag
    11341 tttgtgctct tatatgtgtg attgaaaata tagataaaaa aacaatcatc ggctttgact
    11401 tcgatgattg ttttctttat tttgtaaaaa tcctatcact tgattttatg gtaacctttt
    11461 atttttgtct tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag
    11521 acataccaga aaatcgtacg tccgatacca taatgttgat agagctttgt caacatcacg
    11581 tgtaacaaga tcaaccctaa aagcaaggag ccagctataa accaatgatt atcccaatgc
    11641 atgacttcac taagtgccat cactccaaaa ggatagaaat aaaaatagat cccgactaag
    11701 gtcatccgcc agttctttgt aaatgttccg atattcaata aaaagaaaat cgctgaaagc
    11761 aaaataccga taggcggaaa aagcccaacc aaaaaggaat acaacatccc ccacaagatc
    11821 atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt
    11881 tgtgataacg ttgcaacagt cattaatcca aataaaatta atgtgaacca atgccaatgg
    11941 gactgctgtt gttctttacg ggaaaaatcg acccggttgg ataaataatc tgctaatttc
    12001 ggataagaac gcttaaaatc cataaactgt tcctccttcg tggaaaaggt aggtttagta
    12061 tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga gtttttcact gaaagtggag
    12121 aaaaaagaat ggtaaattaa aatattctca gttatactga aaattatacc aaatttcaaa
    12181 gaagaaagag gatgggaatg aaaggcaaac taaaacgaga aatcaatctg tttggcgcat
    12241 tagctaccgt tatgggaacc gtgatcggtg caggcgtctt cttcaaaact gctgctgtta
    12301 ctgctagtac tcagtctaca agtttgacat tgctagcttg gcttttgggt ggtttcttaa
    12361 cgatctgtgc tggattgaca gtggctgaat tagctacagc aatcccggaa actggtggag
    12421 ctgtaaaata tattgaagct gcgtatggaa aattgcccag ctttttatta ggatgggcgc
    12481 aaagcttgat ttactttcct gctaatattg ctgcattatc gattatcttt gcgacacaat
    12541 tgacgaactt attacaatta tctaccgatt atcttttgtt gattgcagtg atcaccgctg
    12601 tttctgtgac agggttgaat ttgttaggga caaaagtcgg tgcttctgta caatcagtga
    12661 cgttgattgt taaattaata ccaattgctg tgattgtcat atggggatta ttgaccccag
    12721 gacaaggaac tgtccagtta tttccaatcg aagctggaaa agatgttact tttgtcgaag
    12781 gattgagcag tgcgttgtta gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg
    12841 caatggcagg ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt attcttggtt
    12901 tgagttttgt aacagtcgtt tatctattga tcaattttgt ttttttgaaa actttgccga
    12961 ttgatcatct tgccggtaat ttgaatgcag cttctgaagc atcagacgtc atttttggag
    13021 gaatcggtgg aaagctggtc actattggga tcttgatctc ggtatacggt gcattgaacg
    13081 gttatacact aactgggatc cgcgtgcctt atgcgatggc tttagaagac gagctacctt
    13141 tcagtaaaca gctgacaaag ctttcaaaga agtttacggt tccttatgtt cctgcggttt
    13201 tccagttagc agtagcttgt attatgatga gccttggttc tttcgacttt ttaacagaca
    13261 tgttgatttt tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc tttattttaa
    13321 gaaaaaaagc gccggaattg ccacgtccat ataaagtacc actttatcca atcgttccga
    13381 ttattgcgat tctcggtgct attttcattt taggtatgac gatgatgacg caaacgaaat
    13441 tagcattgat cggtataggt gttaccttga taggtatccc agtctattat caaaaaaaga
    13501 aaaaacgctc agaagagtaa aatatttcaa caacagacag aaaaagtacg aactaggtcc
    13561 tctagatctt cgtgcttttt ctatttcaat aaaacaagtt cttctatttt gatttttgct
    13621 acttctgcaa gtagtgctta tgtgctaaag taagaatgaa aaggagagat aaacatgata
    13681 tttgattctc atacccattt aaatgctgaa cagttcaatg aagatattcc agaaacgatt
    13741 cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg
    13801 attgaaaagt ctttgcaatt aagccatgac tatccaaata tatacagtat tatcggctgg
    13861 catccgacag aggctggcag ttatacgaaa gaaatcgaaa ataaattaca agaacaattg
    13921 actttaccaa aggttgtagc attaggggaa attggcttgg attatcattg gatggaagac
    13981 ccgaaagaag tccaagataa agtatttcgc agacagattg ctatcgcaaa agaaatgaac
    14041 ctaccaatca gtattcatac tagagaagca cttgaagaca cgtatcaaat cctaaaagaa
    14101 gaagatgttc gagatatcgg tggaatcatg catagtttca gtggtgatta tgaatgggca
    14161 aaacgatttc ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa
    14221 gcattagacg tccaagaggc tgcagcaaat gtaccaatcg aacgattact tgtggaaaca
    14281 gatgcccctt atctggcacc agttcctttt agaggaaagc gaaatgaacc aggctatact
    14341 cgttatacag tagaaaagat tgctgaactt cggaatcttc cgacagaaga agtggctgcc
    14401 caaacatgga caaatgcgca ccgattattt ggtctaagcg aacatggata agatgaagat
    14461 ccaagagatt atagtcgttg aaggaaaaga cgatacaaga aggctgcggg aagtagttga
    14521 tgcagatacg atagaaacaa tagggtctgc aatcaatgaa gagatcctga tgcaaattga
    14581 acatgcccaa gagactcgtg gagtgatcgt ttttaccgat cctgattttt caggagaaaa
    14641 aataagaaaa atcattatgg aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac
    14701 acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg atgctgctat
    14761 tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg tttctgaaat
    14821 cactagacaa gaccttttag actatggttt aatagctggg gcacaagcaa aagaaaaacg
    14881 agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg
    14941 tttgaagatg tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga
    15001 acaacatgag tgaatatcgt gaaattgcga caccctctag aacaaaagag attttattga
    15061 aacatggttt ttcatttaaa aaaagtttag gacaaaattt tctgactgaa ccaaatattt
    15121 taaggaaaat cgtagaaact gcgcaaatag atgaacatac gaacgtgatc gaagttggac
    15181 caggaatcgg tgcattgacg gaacaattag ccaagcatgc aaaacaagtc gtagcatttg
    15241 agatcgatga tcgtttgatc cctgtattgg ctgatacgat gcaaccctat gacaatgtga
    15301 ctattgtaca tcaagatatt ttaaagaccg atttgtcaac agctgttaga gaaacatttc
    15361 atgaggagct tccgttaaaa gtcgtagcga atttacctta ctatattacg acaccgatca
    15421 tgatgcattt tcttgaatca gatttggttg tggatgaact agtcgtcatg atgcaaaaag
    15481 aagtagctga tcgtatctct gctgaaccag gaacgaaagc ttatggttca ttatcgattg
    15541 ctgttcaata ttatatggaa gccagtttag cattcatcgt accaaaaaca gtatttgtcc
    15601 ctcagccaaa tgtagattca gcgattttga aactgacaag aagagatata ccggcagtcg
    15661 aagtgacaga cgaaaaagca tttttccgat tgacaaaagc ggcatttcag caaagaagaa
    15721 aaacgctttg gaataacttg cagcattcgt atggcaaaga tgaccagaca aaagcatggt
    15781 tagcaaaaag cttggaaaca gctggaatcg atccaaaacg tcgtggggag actttgtcat
    15841 tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt
    15901 tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa
    15961 tagttcctca tgtttttcgt ttgtttgggc ttattaccgc agatgcaagc ttttgaacac
    16021 cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa gtcacaagga acaatttcaa
    16081 aaaacagctt ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa gcttttgacc
    16141 actattatgc cgaacaagga gatgtgacac aaattttgtc acatctccta tttttgtttc
    16201 ttgatcaaaa aattcttata cttgatactc acactcattt tgtatggatt tagaaaagaa
    16261 tatgtgtctt gacagttatt agtaagtgag ttatactaag tcgacattta tgtgtcaaga
    16321 catattagga gagagaaaaa atgagaaaaa attcagttcg aaaactaacg atttctgctt
    16381 tattgattgg gatgggggtc attatcccaa tggtcatgcc taaaatcatg attccaccag
    16441 cttcctttac tttggcgtct catgtgccat tatttattgc gatgtttttt acaccaggag
    16501 ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt tttaacaacg ccggttatca
    16561 tcgcattgcg agcattgtca catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa
    16621 agcatccgag tatcgtttta aaaaatggac agtttacttt tgctaattgg cgtttccaag
    16681 ggttcaattt tgtgatcggt gtgattcatt ctttagcaga aatgctagta gtcagcatct
    16741 tttactttat tggtaatatg cctgaaacat attattctca aggatatttt tatacagtat
    16801 tcatcttgat ggggattggt gggcttatcc acagtttagt tgattataat attgcttatt
    16861 ttatagcagg aacactcagc aaatattttg atatccccgt atttacggca gcaaaaaaag
    16921 aaaatcggaa agagtcgatt caagggtacg cgaagaaaac gacatattag acatgatctc
    16981 tcaaaaaaaa aactctcatc tagtaatgga taataaatcc tctaagatga gagttttttg
    17041 tcctaaaaag agctgcacca acatcttctg gcacagctct aacagggggc aatttatgga
    17101 agtttgatga cttgttctgt ttctttatca aagaagtggc ctttgctgat gttgaaggca
    17161 aggtcgacta tttcgcctgg tttatggaaa tctcttgcat ctacttttga gatgaactca
    17221 gtagaaccag tttttgtgta aagcatcgtt tcagctccaa gtaattcgga aacgacaact
    17281 tctgatttca ctacggcttc aggtgaagct tcaagggcta cttgttcact gtggatgtct
    17341 tctggacgaa taccgaagat aacttctttt ccttcataac ctttttcaac taatactttg
    17401 ttttttcctt caggaatacg aagcttaagc ccatgtccat ctgtgataac accatttttc
    17461 aatgtcacat taaagaagtt catggcagga gatccaataa agccggcaac gaacatattg
    17521 acgggtgtat cataaacttc tttcggtgta ccgatttgtt ggataaatcc gtctttcatg
    17581 ataacgattc ggtcagccat agtcattgct tctgtctgat cgtgagtaac atagatggtc
    17641 gttgtttcaa gacgctggtg aagtttggca atctcagcac gcatggctac acgtaattta
    17701 gcatctaaat ttgataatgg ctcatccatt aagaagactt ttgcatcccg aacgattgcc
    17761 cgtcccaatg ccacacgttg gcgttgtcca ccagataaag cagctggttt tcgtttcaag
    17821 tattctgtca gacctaagat ctctgctgca ttgtccacac gttttttgat gtctgcttta
    17881 tcatatttac gtaatttcaa gccaaaagcc atattatcaa aaacagtcat atgaggatac
    17941 aatgcgtagt tttggaaaac catggcaata tcacggtctt taggtgccac gtcattcatg
    18001 acttttccgc cgatatttag ttctccttca gagatatctt ccagtcccgc gatcattcgc
    18061 aaagtagttg actttccaca accggaagga ccaacaaaaa cgataaattc acggtctttg
    18121 attgatagat taaaatcagt tacagaataa ttttctgcat tgtcatattt tttatatacg
    18181 tttttcagag ccatttctac cataatacgt tccacacttt cttttttagt tttattaact
    18241 atttaaagta taaatgaaag cgttaactaa cttcaatgtc atgttgaaca agaatttttt
    18301 cttt
//
LOCUS       DAFGFZ010000057        18108 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000057 DAFGFZ010000000
VERSION     DAFGFZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.057"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(164..388)
                     /locus_tag="LR726_001669"
     CDS             complement(164..388)
                     /locus_tag="LR726_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBL6216763.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="LR726_001670"
     CDS             complement(586..2166)
                     /locus_tag="LR726_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL6216764.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="LR726_001671"
     CDS             complement(2567..3943)
                     /locus_tag="LR726_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL6216765.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="LR726_001672"
     CDS             complement(4071..5189)
                     /locus_tag="LR726_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL6216766.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="LR726_001673"
     CDS             5331..5765
                     /locus_tag="LR726_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6216767.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="LR726_001674"
     CDS             complement(5843..6376)
                     /locus_tag="LR726_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL6216768.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LR726_001675"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LR726_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL6216769.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LR726_001676"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LR726_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL6216770.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="LR726_001677"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="LR726_001677"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL6216771.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="LR726_001678"
     CDS             complement(10101..10679)
                     /locus_tag="LR726_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL6216772.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="LR726_001679"
     CDS             11001..11471
                     /locus_tag="LR726_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL6216773.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="LR726_001680"
     CDS             complement(11579..12805)
                     /locus_tag="LR726_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL6216774.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="LR726_001681"
     CDS             complement(13559..13759)
                     /locus_tag="LR726_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL6216775.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="LR726_001682"
     CDS             complement(13893..14501)
                     /locus_tag="LR726_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL6216776.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="LR726_001683"
     CDS             complement(14586..15056)
                     /locus_tag="LR726_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL6216777.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="LR726_001684"
     CDS             complement(15104..16018)
                     /locus_tag="LR726_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL6216778.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="LR726_001685"
     CDS             16164..16967
                     /locus_tag="LR726_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL6216779.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="LR726_001686"
     CDS             17145..18092
                     /locus_tag="LR726_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL6216780.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5438 a         3631 c         2964 g         6075 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatat
//
LOCUS       DAFGFZ010000058        18035 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000058 DAFGFZ010000000
VERSION     DAFGFZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.058"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(24..722)
                     /locus_tag="LR726_001687"
     CDS             complement(24..722)
                     /locus_tag="LR726_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL6216781.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="LR726_001688"
     CDS             complement(715..2067)
                     /locus_tag="LR726_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL6216782.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="LR726_001689"
     CDS             complement(2290..3690)
                     /locus_tag="LR726_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL6216783.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="LR726_001690"
     CDS             3851..4588
                     /locus_tag="LR726_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL6216784.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="LR726_001691"
     CDS             4713..5192
                     /locus_tag="LR726_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL6216785.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="LR726_001692"
     CDS             5189..5956
                     /locus_tag="LR726_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216786.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="LR726_001693"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="LR726_001693"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL6216787.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="LR726_001694"
     CDS             complement(6859..7461)
                     /locus_tag="LR726_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL6216788.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="LR726_001695"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="LR726_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="LR726_001696"
     CDS             8142..8726
                     /locus_tag="LR726_001696"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL6216789.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="LR726_001697"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="LR726_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL6216790.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="LR726_001698"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="LR726_001698"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL6216791.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="LR726_001699"
     CDS             10627..10806
                     /locus_tag="LR726_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216792.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="LR726_001700"
     CDS             10828..11853
                     /locus_tag="LR726_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL6216793.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="LR726_001701"
     CDS             12028..13272
                     /locus_tag="LR726_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL6216794.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="LR726_001702"
     CDS             13425..14372
                     /locus_tag="LR726_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6216795.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="LR726_001703"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="LR726_001703"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL6216796.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="LR726_001704"
     CDS             complement(15274..15516)
                     /locus_tag="LR726_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216797.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="LR726_001705"
     tRNA            15637..15709
                     /locus_tag="LR726_001705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="LR726_001706"
     CDS             complement(15828..16466)
                     /locus_tag="LR726_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL6216798.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="LR726_001707"
     CDS             complement(16589..17221)
                     /locus_tag="LR726_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL6216799.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="LR726_001708"
     CDS             17572..17871
                     /locus_tag="LR726_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL6216800.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6179 a         3158 c         3559 g         5139 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtat
//
LOCUS       DAFGFZ010000059        17871 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000059 DAFGFZ010000000
VERSION     DAFGFZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.059"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1942
                     /gene="nrdE"
                     /locus_tag="LR726_001709"
     CDS             <1..1942
                     /gene="nrdE"
                     /locus_tag="LR726_001709"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL6216801.1"
                     /translation="YSPSFIEKLYQFLDEQHFSFKSFMAAYKFYSQYALKTNDGSAYL
                     ETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLNAGRKRRGELVSCFLT
                     QVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFE
                     DSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIRVKTLSLGVIVPDKFY
                     ELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNIRKQKIKARDLENEIS
                     KLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPSVINGKQEYEVLGTDI
                     SCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPPIQNGNKLNHTIGLGA
                     MGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIARERKQVFHNFEKSAYA
                     DGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVMKDGLYHQNRLAVAPN
                     GSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETIPYYTSAYDMDMRKVI
                     DVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLNILRHYAFHKGIKSIY
                     YVRTFTDDAEEIGSNQCESCVI"
     gene            1992..2957
                     /gene="nrdF"
                     /locus_tag="LR726_001710"
     CDS             1992..2957
                     /gene="nrdF"
                     /locus_tag="LR726_001710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL6216802.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3046..4185)
                     /locus_tag="LR726_001711"
     CDS             complement(3046..4185)
                     /locus_tag="LR726_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL6216803.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(4494..5207)
                     /locus_tag="LR726_001712"
     CDS             complement(4494..5207)
                     /locus_tag="LR726_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL6216804.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(5461..6162)
                     /gene="nagB"
                     /locus_tag="LR726_001713"
     CDS             complement(5461..6162)
                     /gene="nagB"
                     /locus_tag="LR726_001713"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL6216805.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            6396..7928
                     /locus_tag="LR726_001714"
     CDS             6396..7928
                     /locus_tag="LR726_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL6216806.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8119..9321)
                     /locus_tag="LR726_001715"
     CDS             complement(8119..9321)
                     /locus_tag="LR726_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6216807.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(9403..10092)
                     /locus_tag="LR726_001716"
     CDS             complement(9403..10092)
                     /locus_tag="LR726_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL6216808.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10198..10566)
                     /gene="rplL"
                     /locus_tag="LR726_001717"
     CDS             complement(10198..10566)
                     /gene="rplL"
                     /locus_tag="LR726_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL6216809.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(10619..11122)
                     /locus_tag="LR726_001718"
     CDS             complement(10619..11122)
                     /locus_tag="LR726_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL6216810.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11326..11453)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(11532..12221)
                     /gene="rplA"
                     /locus_tag="LR726_001719"
     CDS             complement(11532..12221)
                     /gene="rplA"
                     /locus_tag="LR726_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL6216811.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12325..12747)
                     /gene="rplK"
                     /locus_tag="LR726_001720"
     CDS             complement(12325..12747)
                     /gene="rplK"
                     /locus_tag="LR726_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL6216812.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13090..13758
                     /gene="sdaAB"
                     /locus_tag="LR726_001721"
     CDS             13090..13758
                     /gene="sdaAB"
                     /locus_tag="LR726_001721"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL6216813.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            13771..14643
                     /gene="sdaAA"
                     /locus_tag="LR726_001722"
     CDS             13771..14643
                     /gene="sdaAA"
                     /locus_tag="LR726_001722"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL6216814.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(14734..15753)
                     /locus_tag="LR726_001723"
     CDS             complement(14734..15753)
                     /locus_tag="LR726_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL6216815.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(15926..16831)
                     /locus_tag="LR726_001724"
     CDS             complement(15926..16831)
                     /locus_tag="LR726_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL6216816.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(16890..17435)
                     /gene="nusG"
                     /locus_tag="LR726_001725"
     CDS             complement(16890..17435)
                     /gene="nusG"
                     /locus_tag="LR726_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL6216817.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(17536..17706)
                     /gene="secE"
                     /locus_tag="LR726_001726"
     CDS             complement(17536..17706)
                     /gene="secE"
                     /locus_tag="LR726_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL6216818.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(17730..>17871)
                     /gene="rpmG"
                     /locus_tag="LR726_001727"
     CDS             complement(17730..>17871)
                     /gene="rpmG"
                     /locus_tag="LR726_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL6216819.1"
                     /translation="KAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYTLHKE
                     TK"
BASE COUNT         5448 a         3666 c         3222 g         5535 t
ORIGIN      
        1 gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac atttttcttt
       61 caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa ccaacgacgg
      121 ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt actttgcgaa
      181 cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac gatatcagcc
      241 agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg tgtcttgttt
      301 cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact ctgctttgca
      361 attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg aagccggcgc
      421 tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga aattattcga
      481 agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg tggtttattt
      541 gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa atgcagatga
      601 aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat tttatgaatt
      661 gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa aagaatatgg
      721 tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg caaatccaaa
      781 cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa aattacaaca
      841 agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga atccaatcga
      901 tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa cgccttctgt
      961 gattaacggc aaacaggaat atgaagtatt aggaacggat atctcatgta acttaggctc
     1021 gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc gtacgatgac
     1081 tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac cgatccaaaa
     1141 cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca cttatcttgc
     1201 aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata tttacttcat
     1261 gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa gaaaacaagt
     1321 tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa aatacgtaac
     1381 tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca tttttatccc
     1441 aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac tgtatcatca
     1501 aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata cgagtgcaag
     1561 tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg gtaaaattta
     1621 ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg cttatgatat
     1681 ggacatgcgt aaagtaatcg atgtttatgc aatggcacaa caacacgtcg atcaaggcat
     1741 gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat ggaagacaac
     1801 aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc acaaaggaat
     1861 caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg gaagcaatca
     1921 atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat tttaattaaa
     1981 ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa attgaagata
     2041 tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat acacgtatcc
     2101 cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa gatacagtag
     2161 gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt ggcatggatc
     2221 agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat attcaattca
     2281 tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat acgaaaaaag
     2341 aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa aaagctgagc
     2401 gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc agcgtctttt
     2461 tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta ggaaacaaca
     2521 aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct gtacatggaa
     2581 cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca gaacaagaaa
     2641 cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa gaacgttata
     2701 cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc ctacgctata
     2761 atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat actgccaatg
     2821 atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat gacttcttct
     2881 cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac gacgattatt
     2941 tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa taaaaagagt
     3001 ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt cacttgtttc
     3061 ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc tcaataaata
     3121 gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta tgcaaatcag
     3181 catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga tcgtcaaagg
     3241 atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat ctaattgttg
     3301 gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa acaaaccgac
     3361 aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat tcgtgattcc
     3421 gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg ccaaaaagct
     3481 cattacacca ataatcaaag catctaagat cgtactgctg atataggcag ataatgtttc
     3541 gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa agaagtgaga
     3601 caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa atggaacggt
     3661 aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac ttgcagataa
     3721 actgactaat atgatattga acaaattact aagagataaa tttgctgatg ctaattgttc
     3781 ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc atttgcttgt
     3841 ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca ttaactgagg
     3901 cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg tcaataaaaa
     3961 accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat cgaacatata
     4021 atagataaaa ccagcaacaa gcaatggtaa aaaaactgcc gaaagcattt tcaatattgg
     4081 actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga gtactagctc
     4141 cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc tcctccaagg
     4201 tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat tttcatttgc
     4261 ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt aaacaaaaac
     4321 acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata aacggtgttc
     4381 aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa ctatttttat
     4441 ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat catttattgc
     4501 gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc ctcgatcaca
     4561 tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta cactcggtag
     4621 tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt ctcttcaaaa
     4681 taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc taacttttta
     4741 gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat cccgataaaa
     4801 ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc agcaattgcc
     4861 cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat gatcgttccc
     4921 gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc atctgaacga
     4981 atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact agcgatctca
     5041 acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt acgcatacct
     5101 gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag catagcgatc
     5161 cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta aacaccctcc
     5221 agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt acctcttacg
     5281 aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac ggtgttcaat
     5341 cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt tttcctttgt
     5401 cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta ctattgaaaa
     5461 ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt gattttgcaa
     5521 tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga ttgcatcagc
     5581 tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg aaccgattcc
     5641 cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt tgtttgcatt
     5701 gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag caccaggttc
     5761 attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga ttggatgtgc
     5821 atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt ttccatctgg
     5881 tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga aatgacggta
     5941 gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag aagtcatttc
     6001 actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg tgcttcctgt
     6061 tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt caaatgcttt
     6121 ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg ctcctttata
     6181 ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta gtatagacca
     6241 aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt ttcataagat
     6301 ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact gtggacgctt
     6361 tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca ttaaacaaca
     6421 ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa ttaaatgctg
     6481 gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg attaaaatca
     6541 tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat attcacaaag
     6601 gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa tttctaaaaa
     6661 ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac caaacatata
     6721 gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg gaattagacc
     6781 aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg ttggggatcg
     6841 atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt tcaaaggtga
     6901 ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa ccaaccaatc
     6961 acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt gacggtgcgt
     7021 atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat atcgcagaca
     7081 tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg aaacaaaagg
     7141 aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa atcgaaaaga
     7201 ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca aaaagccgac
     7261 aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt aaagctgtct
     7321 ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact gatttagtca
     7381 tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt ggtgaaaata
     7441 ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca ctgatcggtg
     7501 agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc aattactttt
     7561 cacaagatct gtactgggaa aatccattag aaacaccact acaatattta agcagtcaat
     7621 ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg ttagttaatc
     7681 aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag gtgaaacttg
     7741 cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg aaccatatcg
     7801 atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga acagtaatca
     7861 tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt gagattgaac
     7921 caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag ttttgtcaca
     7981 actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag ctcaaaaaac
     8041 ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc agaacagctt
     8101 ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt taaaaacaac
     8161 tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta cacctaacca
     8221 gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc caataaagta
     8281 cataaacata aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg
     8341 tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag
     8401 caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac
     8461 aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg
     8521 tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca
     8581 attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc
     8641 aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg
     8701 cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg
     8761 cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata
     8821 gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat
     8881 ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc
     8941 tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa
     9001 agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc
     9061 agataacgct gccattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac
     9121 taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc
     9181 acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat
     9241 attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg
     9301 ttttttttca ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg
     9361 aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt
     9421 tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac
     9481 acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca
     9541 cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag
     9601 aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat
     9661 atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg
     9721 attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat
     9781 gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa
     9841 accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt
     9901 caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc
     9961 tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa
    10021 ccgatagccc actaccagta tcagaatgat tcccaacgag atcaatatca atcggattat
    10081 tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata
    10141 aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta
    10201 tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc
    10261 tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt
    10321 caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc
    10381 tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc
    10441 agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag
    10501 gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa
    10561 tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt
    10621 aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga
    10681 ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg
    10741 ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt
    10801 ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt
    10861 cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag
    10921 cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat
    10981 cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga
    11041 ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt
    11101 ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt
    11161 tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca
    11221 cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct
    11281 ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa
    11341 actctatgcc accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta
    11401 ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact
    11461 agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt
    11521 ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg
    11581 atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca
    11641 tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt
    11701 ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg
    11761 tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct
    11821 tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca
    11881 actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg
    11941 aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca
    12001 gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct
    12061 tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg
    12121 tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact
    12181 ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg
    12241 tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga
    12301 agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc
    12361 ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc
    12421 agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc
    12481 tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt
    12541 aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc
    12601 ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg
    12661 acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt
    12721 aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta
    12781 attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata
    12841 atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt
    12901 tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa
    12961 tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa
    13021 agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag
    13081 gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg
    13141 attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt
    13201 atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc
    13261 tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat
    13321 gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt
    13381 gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga
    13441 acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa
    13501 ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa
    13561 gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc
    13621 ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta
    13681 gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc
    13741 gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat
    13801 caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt
    13861 ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa
    13921 tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca
    13981 aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa
    14041 gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct
    14101 acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg
    14161 cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta
    14221 gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt
    14281 tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa
    14341 gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt
    14401 gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt
    14461 atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt
    14521 gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt
    14581 ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg
    14641 tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa
    14701 gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac
    14761 ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg
    14821 ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg
    14881 gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg
    14941 gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa
    15001 tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat
    15061 accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac
    15121 gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg
    15181 ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg
    15241 ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc
    15301 ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc
    15361 ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg
    15421 gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata
    15481 agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc
    15541 tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc
    15601 aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat
    15661 gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc
    15721 accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta
    15781 ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga
    15841 tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa
    15901 catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat
    15961 ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt
    16021 cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg
    16081 gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag
    16141 tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag
    16201 caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa
    16261 aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg
    16321 ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat
    16381 ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc
    16441 actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag
    16501 acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta
    16561 ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc
    16621 tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg
    16681 ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta
    16741 acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga
    16801 ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa
    16861 tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca
    16921 agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct
    16981 ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg
    17041 acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag
    17101 atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca
    17161 acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc
    17221 atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt
    17281 ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc
    17341 atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta
    17401 tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa
    17461 ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac
    17521 gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat
    17581 tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg tatctttacg
    17641 gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa
    17701 tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga
    17761 ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact
    17821 gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt t
//
LOCUS       DAFGFZ010000060        17241 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000060 DAFGFZ010000000
VERSION     DAFGFZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.060"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            29..466
                     /locus_tag="LR726_001728"
     CDS             29..466
                     /locus_tag="LR726_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216820.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            561..1445
                     /locus_tag="LR726_001729"
     CDS             561..1445
                     /locus_tag="LR726_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL6216821.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1669..4836
                     /locus_tag="LR726_001730"
     CDS             1669..4836
                     /locus_tag="LR726_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216822.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4856..5401
                     /locus_tag="LR726_001731"
     CDS             4856..5401
                     /locus_tag="LR726_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBL6216823.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5429..5884
                     /locus_tag="LR726_001732"
     CDS             5429..5884
                     /locus_tag="LR726_001732"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBL6216824.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5881..6639
                     /locus_tag="LR726_001733"
     CDS             5881..6639
                     /locus_tag="LR726_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL6216825.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6725..7465)
                     /locus_tag="LR726_001734"
     CDS             complement(6725..7465)
                     /locus_tag="LR726_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6216826.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7526..8779
                     /locus_tag="LR726_001735"
     CDS             7526..8779
                     /locus_tag="LR726_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL6216827.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9101..9328
                     /locus_tag="LR726_001736"
     CDS             9101..9328
                     /locus_tag="LR726_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL6216828.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9451..9777
                     /locus_tag="LR726_001737"
     CDS             9451..9777
                     /locus_tag="LR726_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL6216829.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9845..10558
                     /locus_tag="LR726_001738"
     CDS             9845..10558
                     /locus_tag="LR726_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL6216830.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10868..11719
                     /locus_tag="LR726_001739"
     CDS             10868..11719
                     /locus_tag="LR726_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL6216831.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11840..13705
                     /locus_tag="LR726_001740"
     CDS             11840..13705
                     /locus_tag="LR726_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL6216832.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13753..15162
                     /locus_tag="LR726_001741"
     CDS             13753..15162
                     /locus_tag="LR726_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL6216833.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15270..15971
                     /locus_tag="LR726_001742"
     CDS             15270..15971
                     /locus_tag="LR726_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6216834.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16130..16609
                     /locus_tag="LR726_001743"
                     /pseudo
     CDS             16130..16609
                     /locus_tag="LR726_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5754 a         2898 c         3714 g         4875 t
ORIGIN      
        1 atcaaaaaat gcacgtagaa gtagataaat ggagtggttt tgcaaatttc ttttatttct
       61 agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag gaagtacaat
      121 ggatcatttg tatataaaat atattcatca attttccgtt ttggtgttag gcacactaat
      181 tatagcgggg attgtcgcat ttattattca aggccgtgac atattaacag tacatcaaga
      241 gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata acaaaaagtg
      301 gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac ctaagatttt
      361 tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca ttgttttaat
      421 tttcctcagt ttcatagtat ggatgtttta tcttgtcaaa aaataaatga atgtttcatg
      481 aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt aaagaaatca
      541 atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc agttcaggcg
      601 gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg gtgtttgatg
      661 aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg
      721 ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa
      781 tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca ttgggtacgg
      841 tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg atcaatcatt
      901 tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac tattttatga
      961 atgtcgtcgc aattggctct atccctgaat caatcaatga tgtagatgcg gagaagaaga
     1021 cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct tccacacaat
     1081 cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc agtaccttgc
     1141 tgataggact aactaactca gtcggcggtt ttgaaacatt acttccaaat gcaaaagtgg
     1201 atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat acgcttaaaa
     1261 cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc taccttactt
     1321 gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat gttgatggag
     1381 atgaagggga taaattacct gtgacaatca aagtattgcc ctcacattta caggtttatt
     1441 gttaaaatgg aggttgtgac cgaggagttg gcgggagaac accgcttgtt cgggataagg
     1501 gactgtgaca agacttttgt cacagccttt tttttgtaca aaaaagaaat ttacacctaa
     1561 tagaaaaatt tttccataaa aatacatatt ttaaatatga aagtacaatt ggaaatagat
     1621 taaaacgata aaataaaatt gtcaaaacgt cagagaggat ggtggagaat gaatcaatgt
     1681 gtaaaaaagc tgattgcgtc gattttgttt gtcttccctc tcttgatggt gacatccgtt
     1741 tttgccgatg tagagaaaac aaacgaatca acgcaatcag agaatcaaag ttttgacaag
     1801 actacttttt cagaagaatc gattacttct gaaactacag aatctcaaag tgaggcttct
     1861 actaataaaa ggcaagaaga aaataagaca agtgagacct cagaagtagc tgaacaaacg
     1921 tctgatagca gtgaagcggc actggcaaga gctgtttcag cggatgggta cagcgaagct
     1981 gccacaacag aagaactggc gcagcttctc gcagacgaat cagtaactaa gatccgactg
     2041 attcagcctt tgactttaga cagggaatta gagatcaaac gagatatcgt tattgatttt
     2101 ggtggattcg ctcacaattt cggcacacac catatttata tcaatgagat acctcagtcg
     2161 atcgagttcc agaattttaa aggaacagct gcacatccag gtggattgac accaaatatc
     2221 gatggaaatg ccattatcat tgcttatatg accgattttt ggggaagcag atatcatttt
     2281 acgggagaaa taaaatttac cgggacactt gatttgtatg atgggtccaa attaggactg
     2341 atttacgctc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtgtt ggatgtccaa
     2401 ccagtgaagg aaggactgaa tgccgctccg ggtaaagcct atttttgtag aagctatcaa
     2461 ttgaatgtga tcaatggtag tcaactttat ggaccgtatc tagggaaatt ctacggtttt
     2521 ctagattcag gagatgagac tggtagtgga aaaacagcgc caggaatcca tatcttaagc
     2581 ggttcaaagg tctctcttga ttatgatcgg gcagatggcg ttagcgatga aggagaggca
     2641 gtcgatacgc tacctggtaa tgtcatgttt gaagtcagtg gttcgggaag cgaattttcg
     2701 gtaaatacaa agatcaatat taccaatgac aacaatcgcg gaatcatcca aatgagaggt
     2761 tcaggatcga aagtcactgt atcgaatggt gggaaaatca ccatcaatac agcaactacg
     2821 ggcggatttc gcttgcaagg agattctagt caaatccatg tatcaagtgg tggacaaatc
     2881 tcggtcaaaa tggctggaga tgacgatcaa cccgggaaca atgggatgcg ttttgtagga
     2941 aaaggccttt ctttgatcgt agaaggagcc ggatcagtta tcgacatcga taagcagagt
     3001 gggcgttcca gcgcagttcg ttttgagaat ggtacgcaaa agctgacagt aaccaatgga
     3061 ggctcattga gggtaagaaa tacaggtgat ggcaaatctt acgtgaacag actcaatcaa
     3121 ggaaatcaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc aggttcagct
     3181 gattttacag tcactggaga aaaatcaaat atcgatatac gcgcagattc cggagcaact
     3241 gtagatacaa ctggagatat tgatttaaat tttacggcag gtgaaaaaac ttatttagtc
     3301 atggtcggga aaacagggga agataagacc ggcatcatca gtggggatgt gttgaatgtg
     3361 gacttagaat ctcctaccta ttttgatttc caaaacaaac ggattggaag cgagaccaat
     3421 ggcataggcg gctgggtatt ccaaggaaat ggagattctt ctttaaagat cgcccattca
     3481 gaaatcgctt tatggcgaaa aacaggcgca acattcgata actttgaaaa agacccggat
     3541 tatttttctg atatgacaga cttatcgatc aatgggaccg acttaggcaa tttcgtatca
     3601 agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat gaaaaattat
     3661 aatcgaatca gtgccaataa tcagctcccg atgattgaat ccttaagagt gccaacgaat
     3721 gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga
     3781 gatgcttgga caggcgaagt agaagtgact ttacgtatta cttacgcaga tacgacgaaa
     3841 tcatctgtag aaattactgc tctcacgcaa ggacaaacag atgaaaatga aggggcagga
     3901 tataacccat ggggagaagg cgaacaaggc ggcttgttcg aagttcaagt acccaatgat
     3961 gagagattaa aaacggagga tcaagtgcaa gttgttgctg ctaaaaaagt aagaggtggc
     4021 caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc tcctgaacct
     4081 gctaatttaa gtaccacatt gatcaatgca gcttctcgca cgatcacagg tacaggtgaa
     4141 gcaggagcct tggtttcatt gaaaaaaaat caagctgttt tgtcagaaac aactattgat
     4201 gatacagggg aatttgttct ttctatccca gaaaatcaac tttttgcagg agatgtatta
     4261 gaaattgtat taaacgacca agcaggagaa gctactgcta agggtgtgca gaacaagcca
     4321 agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat
     4381 gccccgcctt ttgcacctgc tacgaagatc gtagtagaag gtggtctctc atttcttgcc
     4441 ccttccaaac tcgattttgg tcagatcaaa gcaactggtt tgacacaaga agcatttggc
     4501 caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacgaa aaatccgtgg
     4561 atcttaacga taaaattatc tcagcctttc acaaatgatg caggattttc tatgcaggat
     4621 gtcctatatt tcaagaaagg agaggagtat caattagtga atgaagaaga actggaaatt
     4681 gtcagaggac aaaattcaac gaatgtgcaa gcagattatt cagaatattt aaataacggt
     4741 cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag
     4801 ataacatgga cattagcaga tgcgccgaca gaataaagat caaggaggat gattgatgaa
     4861 tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttatgatcc ttctccttgg
     4921 attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga
     4981 attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg
     5041 gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga
     5101 gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa
     5161 aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga
     5221 aataactgtt cctcctcaaa gtgcggtcac tgagacactt tcactgaaag cgccagaaaa
     5281 agagctggct ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag agaacgtttc
     5341 taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgatttg
     5401 aaatagcgaa aaagagtctt ccccagattt gaaattaaaa aaagcttatg cggatcaaag
     5461 cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg
     5521 cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc tttcaataaa
     5581 aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg
     5641 aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg
     5701 ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa
     5761 ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg
     5821 agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa
     5881 atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa
     5941 agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc tgtgaccaaa
     6001 ccagatgctg caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatcct
     6061 gatactcctt ttacacctga cgaaccaaat cagggaacat ccggattact gacgattgac
     6121 caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct
     6181 gcattacaaa gcggaacgga cagtactgga gcacaaacac aagtacagaa tttcgtacaa
     6241 gtaacggata aaagcggaaa ttatgccggt tggaaattat cagtcaaacg aacagaattt
     6301 attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc
     6361 gtgattcgaa atagcgcaac taatagcagt ggaatgccat ctaccgtttc tggaactggc
     6421 gctgaaggaa tcgaaatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat
     6481 gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca
     6541 gtggaattat ttgttcctgt atccaattat gctgtagaca actacgtttc aacattcaca
     6601 tggtcgttaa cagatgctcc tacagaagag gaagcgtaaa atcatatcaa cgatttcagg
     6661 gagtggagcc gattcaaggc ccactccctg aaatctgcta tgcttacgtc tcagctctta
     6721 ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga
     6781 tctttcgacc aacgagtaag gagtcaccgt tgaaaaattc taacacataa tgagcagaat
     6841 caattttctg caattgcgat aagttgatac agtaagattt taaaagtaaa aagtaagcat
     6901 acttttctaa ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag
     6961 ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc
     7021 gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga
     7081 tctgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc
     7141 tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat
     7201 tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg
     7261 cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat
     7321 tatcaatagt gcccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga
     7381 tattctcgat atgatgattg accaattctc tgatgatcca ttcgctgtga aactggttat
     7441 cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt
     7501 tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac
     7561 tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag
     7621 ctttggttgt atagctttga tggtggtgac acttttactt cctatcccta aagtgagctt
     7681 tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt
     7741 tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt
     7801 ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact
     7861 acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct
     7921 caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag
     7981 ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct
     8041 tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat
     8101 cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga
     8161 agaatattac catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt
     8221 aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tattagaaga
     8281 tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc
     8341 agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat
     8401 ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg
     8461 ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat
     8521 tacgatcaat ctgctggaag aaaaagaggg atcacgtttt agtatagcga acaaggcaaa
     8581 tccaacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg
     8641 cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa
     8701 aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa
     8761 gagcaaatca gcctactgac ttactgtcaa aggataagtt tgaggaaagt ctacacggag
     8821 atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct
     8881 tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag
     8941 tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg
     9001 tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta
     9061 ggcgtggaga agcaagcaaa tccctacgat cacaaaaaaa atgctgaccc agcagttaag
     9121 agagttagaa caagatgaaa gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt
     9181 agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag cgttgtctgt
     9241 ttgggaagaa gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca cgctactgca
     9301 tacacatggt ggctacctaa attattgatt ttctatgaca tttattttaa ataaaagaaa
     9361 acaggagttt ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca
     9421 agtaagaagt gattggaaga aggcaagcaa atgaatagta tggaaaaagt caaccagttt
     9481 agagatgaac gtaattggcg acaatttcat aacgaaaaag atttggctat ctctatttct
     9541 ttggaagcat ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa
     9601 acacaattag aacgaatcaa agaagaactg gcagatgtat tgatctattc atacatgatg
     9661 gcagacaacc ttcacctgga tctagatgag atcattgaag aaaaattgat caaaaataat
     9721 ttgaaatatc ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa
     9781 tgtgtcattc ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga
     9841 gaaaatgaaa aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt
     9901 gttattagta ggactagctt tgattttcaa tgaacagatc aaggattatt ttgtcagaga
     9961 gacaggagat aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga
    10021 taaagatgtc agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag
    10081 gtcgcaaatg agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc
    10141 aatcaattta ccggttttca aaggcttgga taatacgtca ctactttacg gagcaggaac
    10201 attatctcca gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac
    10261 gaaccctgaa ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt
    10321 gacagattta gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac
    10381 agatacccaa ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg
    10441 cgacatggac gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacatcgat
    10501 aaaagatgcg acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga
    10561 tactgggaca taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa
    10621 aaccaatttg ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt ggttgttttc
    10681 tagatagact tgtgttccag tttttttatt ttttatatga aaaggcttgc ataaataaaa
    10741 attcgatggt atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc
    10801 agaattcagc taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg
    10861 aagcaaaatg aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt
    10921 cggggaagaa gtcgtttgta tgggaaaagg actagctttt caaaaaaaga ttggggacga
    10981 gatttcgcca gaagcggtcc aaaaagaatt tgttttaaga gattcttttg ctaaaaacca
    11041 attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt
    11101 cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac
    11161 cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt
    11221 attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt
    11281 gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt
    11341 tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac
    11401 aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga
    11461 atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa
    11521 aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc
    11581 aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga
    11641 aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa
    11701 aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa
    11761 taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa
    11821 aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt
    11881 aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt
    11941 aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt
    12001 cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca
    12061 atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa
    12121 tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat
    12181 ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac
    12241 agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt
    12301 acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt
    12361 agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc
    12421 tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat
    12481 accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat
    12541 tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt
    12601 aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt
    12661 caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca
    12721 agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga
    12781 acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg
    12841 ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc
    12901 ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc
    12961 tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg
    13021 attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt
    13081 cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat
    13141 tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga
    13201 caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac
    13261 cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt
    13321 ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc
    13381 acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac
    13441 gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa
    13501 tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg
    13561 ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac
    13621 gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga
    13681 agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa
    13741 aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac
    13801 ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc
    13861 ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga
    13921 ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc
    13981 aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact
    14041 cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat
    14101 attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac
    14161 ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta
    14221 gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt
    14281 cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat
    14341 attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac
    14401 gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc
    14461 gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc
    14521 tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa
    14581 tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa
    14641 tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat
    14701 ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag
    14761 gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta
    14821 tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat
    14881 atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc
    14941 gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga
    15001 tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat
    15061 gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat
    15121 tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa
    15181 aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt
    15241 ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag
    15301 tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc
    15361 cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa
    15421 tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc
    15481 taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga
    15541 actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg
    15601 aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt
    15661 tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca
    15721 gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta
    15781 ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc
    15841 tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg
    15901 aaatgattga atattcggag gcggtctacc atacggagct gcatccttac catattgatt
    15961 atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
    16021 agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca
    16081 cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg
    16141 aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac
    16201 gatcgcttat ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga
    16261 tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat
    16321 cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg
    16381 cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt
    16441 cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac
    16501 aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga
    16561 agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa
    16621 tggattacaa gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc
    16681 agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag
    16741 catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag
    16801 ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa
    16861 aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt
    16921 acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca
    16981 ctgaattttc ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc
    17041 gttaacaaaa agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt
    17101 agttcgtcgg cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac
    17161 aaaatgattt tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta
    17221 ttggcatata ggacgaaatc c
//
LOCUS       DAFGFZ010000061        16820 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000061 DAFGFZ010000000
VERSION     DAFGFZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.061"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..456)
                     /locus_tag="LR726_001744"
     CDS             complement(<1..456)
                     /locus_tag="LR726_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL6216835.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="LR726_001745"
     CDS             complement(561..1424)
                     /locus_tag="LR726_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL6216836.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="LR726_001746"
     CDS             complement(1620..3719)
                     /locus_tag="LR726_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL6216837.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="LR726_001747"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="LR726_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL6216838.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="LR726_001748"
     CDS             complement(5072..6913)
                     /locus_tag="LR726_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL6216839.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="LR726_001749"
     CDS             complement(7095..9410)
                     /locus_tag="LR726_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL6216840.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="LR726_001750"
     CDS             complement(9595..10455)
                     /locus_tag="LR726_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL6216841.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="LR726_001751"
     CDS             complement(10514..11035)
                     /locus_tag="LR726_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL6216842.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="LR726_001752"
     CDS             11313..12074
                     /locus_tag="LR726_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6216843.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="LR726_001753"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="LR726_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL6216844.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="LR726_001754"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="LR726_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL6216845.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="LR726_001755"
     CDS             16434..16790
                     /locus_tag="LR726_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL6216846.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3491 c         2946 g         5766 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gcataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa
//
LOCUS       DAFGFZ010000062        16177 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000062 DAFGFZ010000000
VERSION     DAFGFZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.062"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            226..894
                     /locus_tag="LR726_001756"
     CDS             226..894
                     /locus_tag="LR726_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL6216847.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="LR726_001757"
     CDS             complement(1094..1324)
                     /locus_tag="LR726_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216848.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="LR726_001758"
     CDS             complement(1451..2833)
                     /locus_tag="LR726_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL6216849.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="LR726_001759"
     CDS             complement(2989..4176)
                     /locus_tag="LR726_001759"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL6216850.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="LR726_001760"
     CDS             complement(4183..5214)
                     /locus_tag="LR726_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL6216851.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="LR726_001761"
     CDS             complement(5230..6684)
                     /locus_tag="LR726_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6216852.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="LR726_001762"
     CDS             6859..7764
                     /locus_tag="LR726_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL6216853.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="LR726_001763"
     CDS             7855..8532
                     /locus_tag="LR726_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL6216854.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="LR726_001764"
     CDS             8529..8699
                     /locus_tag="LR726_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216855.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="LR726_001765"
     CDS             8813..9709
                     /locus_tag="LR726_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL6216856.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="LR726_001766"
     CDS             complement(9764..11134)
                     /locus_tag="LR726_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6216857.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEILYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="LR726_001767"
     CDS             11372..12118
                     /locus_tag="LR726_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL6216858.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="LR726_001768"
     CDS             12115..12954
                     /locus_tag="LR726_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL6216859.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="LR726_001769"
     CDS             12955..13905
                     /locus_tag="LR726_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL6216860.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="LR726_001770"
     CDS             14046..14798
                     /locus_tag="LR726_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216861.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="LR726_001771"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="LR726_001771"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL6216862.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5252 a         3013 c         3245 g         4667 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cgcaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agtatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa catagaaaaa cgattacttt gcttata
//
LOCUS       DAFGFZ010000063        16160 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000063 DAFGFZ010000000
VERSION     DAFGFZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.063"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..210)
                     /locus_tag="LR726_001772"
     CDS             complement(<1..210)
                     /locus_tag="LR726_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216863.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="LR726_001773"
     CDS             complement(216..446)
                     /locus_tag="LR726_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216864.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="LR726_001774"
     CDS             1063..1725
                     /locus_tag="LR726_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216865.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="LR726_001775"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="LR726_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL6216866.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="LR726_001776"
     CDS             5145..5459
                     /locus_tag="LR726_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL6216867.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="LR726_001777"
     CDS             5472..5846
                     /locus_tag="LR726_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL6216868.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="LR726_001778"
     CDS             5856..6200
                     /locus_tag="LR726_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL6216869.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="LR726_001779"
     CDS             6271..7623
                     /locus_tag="LR726_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL6216870.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="LR726_001780"
     CDS             7683..8825
                     /locus_tag="LR726_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBL6216871.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="LR726_001781"
     CDS             8850..9104
                     /locus_tag="LR726_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216872.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="LR726_001782"
     CDS             9360..10544
                     /locus_tag="LR726_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL6216873.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="LR726_001783"
     CDS             10541..10678
                     /locus_tag="LR726_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL6216874.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="LR726_001784"
     CDS             10683..10904
                     /locus_tag="LR726_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216875.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="LR726_001785"
     CDS             10917..11417
                     /locus_tag="LR726_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL6216876.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="LR726_001786"
     CDS             11514..13361
                     /locus_tag="LR726_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBL6216877.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="LR726_001787"
     CDS             13364..14260
                     /locus_tag="LR726_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6216878.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="LR726_001788"
     CDS             14310..14699
                     /locus_tag="LR726_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL6216879.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..>16160
                     /locus_tag="LR726_001789"
     CDS             14686..>16160
                     /locus_tag="LR726_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6216880.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         5433 a         2602 c         3521 g         4604 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg
//
LOCUS       DAFGFZ010000064        16102 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000064 DAFGFZ010000000
VERSION     DAFGFZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.064"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..359
                     /locus_tag="LR726_001790"
     CDS             <1..359
                     /locus_tag="LR726_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL6216881.1"
                     /translation="DSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTT
                     SDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILL
                     VIGLRITFMSYKKSKR"
     gene            complement(514..1500)
                     /locus_tag="LR726_001791"
     CDS             complement(514..1500)
                     /locus_tag="LR726_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL6216882.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1523..2089)
                     /locus_tag="LR726_001792"
     CDS             complement(1523..2089)
                     /locus_tag="LR726_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL6216883.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2103..2519)
                     /locus_tag="LR726_001793"
     CDS             complement(2103..2519)
                     /locus_tag="LR726_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL6216884.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2976..4910)
                     /locus_tag="LR726_001794"
     CDS             complement(2976..4910)
                     /locus_tag="LR726_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL6216885.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5191..6147
                     /locus_tag="LR726_001795"
     CDS             5191..6147
                     /locus_tag="LR726_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL6216886.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6237..7340
                     /locus_tag="LR726_001796"
     CDS             6237..7340
                     /locus_tag="LR726_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL6216887.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7353..8306
                     /gene="menA"
                     /locus_tag="LR726_001797"
     CDS             7353..8306
                     /gene="menA"
                     /locus_tag="LR726_001797"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL6216888.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9120..10466)
                     /gene="gor"
                     /locus_tag="LR726_001798"
     CDS             complement(9120..10466)
                     /gene="gor"
                     /locus_tag="LR726_001798"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL6216889.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10600..11853)
                     /locus_tag="LR726_001799"
     CDS             complement(10600..11853)
                     /locus_tag="LR726_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL6216890.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11859..12782)
                     /locus_tag="LR726_001800"
     CDS             complement(11859..12782)
                     /locus_tag="LR726_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL6216891.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13003..15495)
                     /locus_tag="LR726_001801"
     CDS             complement(13003..15495)
                     /locus_tag="LR726_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL6216892.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15502..15969)
                     /locus_tag="LR726_001802"
     CDS             complement(15502..15969)
                     /locus_tag="LR726_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL6216893.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4726 a         3361 c         2862 g         5153 t
ORIGIN      
        1 cagattcatc taccactaca agtgatagca cggacacgtc agcttcaagt gacagtacag
       61 atacatcctc taccacaagt gatagctcag attcatcaac ttcaagcgat agtacagaca
      121 cgtcgtctac cacaagcgat acacgtcatg caagtgtctc cttctcaaaa acaagttcgg
      181 ataacaggac gaactttaac ttaactaata aaacaagcaa tgacaatgga aacaaatcaa
      241 aaagagcact tcctaaaact ggaagtcaat cgaataattg gataacactc gcaggcgtta
      301 tattacttgt aatcggttta agaatcactt ttatgagtta taagaaatcg aaaaggtaaa
      361 aaaatattca agataaaaaa gagcaacgtc gctaacggac gttgctcttt atttttgctt
      421 tttatcataa atacaagaac tacaattcaa tttgtcaaaa aacacgtaga tttccttctg
      481 agaaatttat ttacgtatcg aacctttccc tattcagtct ttgcgatcca aaatcagttg
      541 ggtaagggaa aagagattct cttttgttcg aagagaggtg gcaggtaatt tctttatttc
      601 cttcaaagcc ttttctgtgt aagaggccgc taacttccta gcttcttcca cgcctcctgt
      661 atgttcaacg attttgcgta cggcgtcgac atcttcttct gtcattgccg ctttcttctc
      721 taaataaggt agtagctttg gtttgttttt ttgcaaagag tagatcaacg gtaaagaata
      781 gaccccttga cgcatgtctt ctagtacagg tttgccgatt tcctggctct cttttgtata
      841 atctaaaata tcatcgataa tctgaaaggc catcccgata gaaagtccga tatctcctgc
      901 tttttttgcg aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac aacttaattg
      961 gaacagttca gctgtcttac ctgaaatatt ttccaaatat tcttttacag aaagttcata
     1021 attatagcga tcatccatct gccctaattc gccattcaat actttttcca tactgcgaga
     1081 attcaattgg agacttttca aggaagtgga gtaatcagac aatagtttaa aacagcagac
     1141 aaataaatag tctcctgcat agacagaaac gacattacca aactgactac ggatattagg
     1201 ctgaccacgt cgcagatccg cttcatcaac gatatcatca tggatcagtg tggccgtatg
     1261 gagcatttcg atagaagctg ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa
     1321 ttgtgagaac aatagctgat aggcaggacg caacagcttt ccgccggcat ggatcgtttc
     1381 tttgattgct tgttcgacag gtttgttttt caggcggaca gtttcttcca tccgttttag
     1441 tgtttctgtc aattctttac ttaaagcagg atagttttcc cacattggat gtagcttcat
     1501 aaaaaaactc cttgtgtgac gattattttt tggtaaacat tgggctccat ttttgtttgg
     1561 ttccctgctc ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat
     1621 tcgctgcgat cccgattgca atccctgata aaatccctaa aactgaaagg actggcaagt
     1681 acaacatgac tgtccatgat tgtgcaatga aagaagccat caaaagctgt ccgacattat
     1741 gcatgaaacc gccggccgca ctaattccga tgatactgac acgttttggt cctaattgct
     1801 tgaccaataa cattccaaaa taactaagca aagcaccact catactataa aggaatgtgg
     1861 aaatcgttcc gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt aagaagctgt
     1921 ctcgtttttt catggtaaaa atggcaacga tcgtgattag attggctaag cctagttttg
     1981 caccaggcgc aaaagaaaaa ggataaggga tcatattttc caataaacca atgatcactc
     2041 cttgtgcaac aagtagcgaa atataaatca ttttttgtaa tttactcatg gtgtgtgaag
     2101 gtctaataaa tcagactgtc ttcgtcacct ccatctgttg aacggacttc gatcactaat
     2161 ttatgtggca aacagacgat ggtttcgcca tttttcgttg cccagcctcg acgtacgcag
     2221 atctggtcat cgcaatctgc ttcttttatc cgtactcgat cgccatctat ctcgatcaag
     2281 ttataatctc cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc agccatcaaa
     2341 gggaatgttt tgatttctgt gccatccaca cgcagtaccg cttctttttc tgctgttgcg
     2401 gtgttttgct gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa
     2461 ataataaaaa catcccaagg tttgagcaga cttttttcag caaattcttt aaggctcatt
     2521 tttgttctcc tttttccaga ttaactcacc caattttagc ataaaaacaa gaggttgtga
     2581 cacctgtgtc ttgctttgag taataagaac gaagaataga aaacagcttt ccatgttttc
     2641 taattctttg ggcttattac cgagaagcag caggtggaac accgtttata acaagaggtt
     2701 gtgacagaag tgggcaactt caagaaataa gaaggaattt acgaaaatta cttttcaagt
     2761 ttttgtgaaa tttcggctta tttttcgaag aagctacttc tggaacaccg tttataacaa
     2821 gaggttgtaa cagaggttgt gacacctgtg tcttgctcat ggaggcttat ctcgggcatc
     2881 ttattccttt aattttacat aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat
     2941 tgaaatcttg cattcaaata acgttcttta tctatttatt tcttgccgta ctgggaactt
     3001 ggtctaccgt accaccaatt acctagtgta cgttgtagcc atggaattac ccagcgatcc
     3061 aacccaatcg cacgtccgga accgttcatc aatgcgaaag caacaaaaat gaaccaaatg
     3121 tttacccagt aaaacatgcc tgataagcag aacgtgatca ccaatacaat cgttgtcgca
     3181 ctgcatagcc atgtgaataa tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat
     3241 accatgaatt tttggaagaa cattgctaca tcattgtttg gcatgatcac ttgcatgatc
     3301 tttgtgaacc agtctggtga cttctcaatc acagccattg gttcttcacc gtatgcataa
     3361 ctcaaaccaa aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc tgctccgctg
     3421 gcagctgtgg ttgcatcacc agcagcagaa gcaccactgg caacatctgt tacattgtat
     3481 tcccatgtcc aagggaacac actgtttcca gtgaaccatg atccttcacc aaaccaagta
     3541 cttggtttga acacttcacc attcccaatg atttttttgg aagcttcaac gaaccaaact
     3601 aaaccataga aaatacgaag tggcacactc cataagacat tgccgtaacg agaagaatgt
     3661 cctcgagtca cgttgcgatc atctttgata tggaaaaact catgcattaa atattggaac
     3721 atataataac cagaacgaat atcaaagaag tatttcaaat tcacgatatg tttcattacg
     3781 atagctaaaa atccgcttaa atggatctta tcaaataggt tagctacccc ccatttggca
     3841 ccaatagaaa ccataaatcc ttgatagttt cctttaaatt tatgcttttc tccgcctttg
     3901 atcgaagcca cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc
     3961 tgcggtgtcg gtgttcctgg gaattcttca taaaagacta aatcaccaat gatatagatg
     4021 tttttatctt cgtagccttt tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt
     4081 gctgattcta atccaaagtc agcagcgtca gaagttcctt tgacaccagc tgtccaaatc
     4141 agtgtgtgag tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc agccacttct
     4201 acgattggtg cattcaataa taattccacg ttgtgcttac gcaaatagcg ttcagctttt
     4261 gcagcatcat ttcgatccaa catattcaag atcgtcggca ttgcctctac gaccatcagt
     4321 gtgatttcgt ttggatctaa tttataatct ttggctaggc gatttttcca gtcgatcagt
     4381 tccccaatca tttcaatacc ggtaaatcca gaaccacaga caacgaaagt cagcatggct
     4441 ttacgcagtt catcatctgg ttccaatgca gcttttgcta ctgtcttctc aatatgttcc
     4501 cgaatcctta cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt
     4561 gtaccaaagt cattcggctc tccgcccatt ccaaggatca gctgatcaaa aggataggaa
     4621 cctaatttgg tcttgacgat ttttcgttct ttatcgatac cgattacagt gtctgtgaca
     4681 agttgtacat tcttttgacg agcaaacaaa tgctgcagat cgtattggat tgcttctggt
     4741 tctacacgtc cgccagctac ttcatgcaac tcggtcatca tagtatggta cgaatgacga
     4801 tcgatcagag taattgctac atcttctttt ttcagtttct ttgccaagta ttttgtagct
     4861 gaaacaccgg catatcctgc tcctacaacg acaatatttt gctttgccat tgatcatccg
     4921 ctccttatag actatctata taatgaatat atgataattc ttttagtttg atacttatta
     4981 cacaatctcc atatattata tagatgttct gttcttttgt ctaactcctt ttggattttt
     5041 ttcaaatttt aaaataaagc gttttttcac gaaatttgct gaaagaaaac gtttgaaata
     5101 ttacatgggt tatttatcaa aaatagttga cttataggct aaaaagatta gaatgtataa
     5161 tgaatcacaa agaaaaattg tggactagaa atggaatggt ggggtattaa aatgaagaaa
     5221 aaacgttttg taactggatt tgccgtatta gcattttcag ctctagtgtt aggcgcttgc
     5281 ggcgcagatg acaacggaag ctcaaactct tcttctgaat catctacagc acaatcttcg
     5341 actgcaaaaa gcacaacaga atcatctgca aaagttgtag ctggcggaga tttgcaagac
     5401 ggcacatata aattagaaga aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg
     5461 gttgtaaaag acggcaaaat cactgaatca aaatatgata acgtcaatga aaacggcgaa
     5521 tcaaaaacaa aagatgcaga atacaacaaa aacatggaag caaaatctgg cacaaaccca
     5581 gaaaaataca ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg
     5641 gaagtcgtaa ctggagctac tcactcatct gaaagcttcc aaaactacgc acaacaattg
     5701 atccaagctg ctcaagctgg taacacagat acaatcgaaa tcgacaatgg tgctgatttg
     5761 aaagacggca cttacaagct tgaagaaaag aactactcta acggctaccg tgtgcaattc
     5821 gaaatgacag ttgctggcgg caaagtaact gaatcaaact ttgattatat cgacaaagac
     5881 ggtaaatcaa aacaagatga tacagaatat aatgaaaata tgaaagcaaa aagcggtaca
     5941 gaaccaaaaa cttacatccc tactctgaac gacgaattcg tcaaagcaat gggcgaagaa
     6001 gatggctcac ctgctaatgt agaagttgtt acaggtgcta cacacagctc acattcattc
     6061 atcatgtacg cacaacaatt agtgaatgca gctgaaaaag gcgacactca aacaatcgaa
     6121 gtagataata tcgtaacaga aaaataatca cataatcata ctaataaaag ttggagggtg
     6181 ggacgatagc cagattaggc agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc
     6241 ggaaaaaact actattttcc ttaatcacac tgggcatctc ttttatggtg ctaactggct
     6301 gcagcaactc agcaaataaa acaaccactg aaagttcagc agctaccaag atattgaaag
     6361 atccatattc tgatgaacag ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg
     6421 aaggcaaaga atcagcattg aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa
     6481 tcacgatcaa tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct gggatcaaac
     6541 cagtaaaagt ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa tatagccaag
     6601 attctcaagg cggattcgat atggcgattg gtgcaatcac acaattatgg cgaatcggtt
     6661 ttgatgacgc cagaaaacct tcccaagaag aaatcgatca ggcattgaaa ttagtggatt
     6721 atcataagat cgaactaaat gacaaagaaa aaactgttta tctaaaagaa aaaggaatga
     6781 tcatcgattt aggtgcaatc gccaaaggtt acattacgga cgaagtagtg aaagtcttga
     6841 aaaaacaagg agtgaccact gccatcgttg acttgggcgg taatgtctat gtcttaggcc
     6901 acagcccaag aggcgaaaac caagactgga cagtcggcat ccaagatccg aacatggctc
     6961 gcggatctgt tttaggttct attaaagaac ggaacaaaac attagttacc tctggaattt
     7021 acgagcgtta tttagaagta gatggcaaaa aataccatca tttgtttgat ccaaaaacgg
     7081 gttatccgtt cgacaatgat atcgcaagtg tcacgatcat aacagataaa tcaattgatg
     7141 gtgacgggtt gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg
     7201 aaagcgaatt gaataacggg acggaagcga tcttcgtgac aaaagacgat aaagtatatg
     7261 tgaccgatcc aatcaaagac accttcaaat tatcggagga ctcccattac acgatgggtg
     7321 accgttctga actaaaataa ggagtacgaa ccatgacact aaaaatcttt ttagaagtag
     7381 ttgaaatacg aacaaaggtg gctagtgtat ttccttttgc ggtaggggta ttattctcta
     7441 tcgctttttt tcatgagata cattggctaa acactttttt attttttatt ggaatgctag
     7501 tttttgattt agcgactacc gctatcaata attacatgga ttatgaaaag gctcattctg
     7561 aagtatataa gtatgaagaa aatgtcattg gccgcagaaa tctatctcct gtattgattc
     7621 gaaacatgat tttagctatg attgcatttg tttttgttat cggcgttatt ctaacgatca
     7681 atacgggttg gcttttactt ttgatgggat ttatctgttg ttttattgga gtattctata
     7741 cattcggacc gattccttta tctcgaatgc ctttagggga aattttcagt ggggtgacta
     7801 tgggccttgg aatttttgcc atggtcgttt atgtcaatac attcggttca gatgtctatg
     7861 atctgacagt caacttttca tctggacaat ttgctttggt aggaaatctt tgggctatag
     7921 cagcaatcgt attagcttcc ttacccttgg tgtttacgat tgccaatatc atgttggcta
     7981 ataatttgcg ggatcttgac cgtgatattg aaaatcatcg ctatacacta gttttttata
     8041 tcggacgtcc tgcgggaaca gtcttattcc agctattgat gtatgcttgc tatttcgtga
     8101 ttttaatggg gttgttgagc ggtatctatg aatggccgat cttggtcacc ttcttgacct
     8161 tgccaattgt gaagaagcat ttggatcaat tcaagcaaac gttaccccag cctatcagtt
     8221 tcagctattc catcaaaaat atggtcttat tcaatgccag ttatgtattg gggctattac
     8281 taacaatcat tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca
     8341 tcaagtaata agaacagcca aataaaaata gctccttata tttttcgttt ggttgggctt
     8401 attaccgcag atgcaagctt ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta
     8461 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
     8521 cttaataccg agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt
     8581 tgtcctgagt attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga
     8641 gctaataccg aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga
     8701 cacaattttt ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg
     8761 agaactttct tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct
     8821 aatttattta tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc
     8881 ttttaaacat cgataccaat attttgaact taagagtgca taccaaatgg attcatttac
     8941 tcttcgtcag ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga
     9001 attatcgaag caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact
     9061 gattttctgg ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat
     9121 tatcgcatcg ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag
     9181 tcttctttag ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca
     9241 ccaacaccaa tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc
     9301 atttcgcatt tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga
     9361 taaactttca attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt
     9421 gttgcaattg gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa
     9481 tctttctttc cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga
     9541 gtcagatcta atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt
     9601 tggtatttat ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga
     9661 cctaattttt cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag
     9721 gtttcaccat tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca
     9781 atatgatgtg cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa
     9841 agcattttat caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta
     9901 cctaaaccac aaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt
     9961 gctctcttag gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca
    10021 ccaggaatgg tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga
    10081 taggtttcac cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata
    10141 gcctcgacat gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat
    10201 ttttctcgtc tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg
    10261 taacttgagg catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt
    10321 ttaggtacac aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa
    10381 acttttgcgc catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca
    10441 attacgatgt aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac
    10501 ctttatttaa cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta
    10561 tgcgaagatg cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat
    10621 atctccaggt ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc
    10681 tcggtcaatg gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact
    10741 gaatcctgga ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat
    10801 cgtagcaatc tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg
    10861 gattttacga cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc
    10921 tgtagctaat tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac
    10981 atagttttca ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt
    11041 agatacataa tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc
    11101 aatcaattct ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc
    11161 ttccacttcg cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa
    11221 tccatacttc cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat
    11281 atggttggac aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc
    11341 ttcgatttcg gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg
    11401 agctaaaacg acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt
    11461 tgctgaagct tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc
    11521 gatcaaagct ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa
    11581 aaattcccct gctccatctt catttccttc atgggtgacc atattagcag caacgtatac
    11641 cttcgcatca tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt
    11701 acctgcacgg cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata
    11761 atggatcgcg gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt
    11821 caattttctt tttactgtca tttctttcat catactcctt atttgatagc agaaggatca
    11881 atataaaaga atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta
    11941 gtcatttttt ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg
    12001 gcgaatagtc gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc
    12061 catgtacagt aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg
    12121 ctggcaaaaa tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt
    12181 ttaggttccg agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag
    12241 ttatagtaat tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca
    12301 agggtttcta caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact
    12361 tcacgagcaa gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg
    12421 ctcgttacta atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc
    12481 atcgtataaa taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa
    12541 aaagccaaat attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca
    12601 atattcgctt ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt
    12661 gtaaatgaag caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca
    12721 cctgcagcaa gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc
    12781 ataattttct cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact
    12841 tcttctattt aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga
    12901 tatattctgt ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc
    12961 aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt
    13021 ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac
    13081 gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc
    13141 tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc
    13201 gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt
    13261 gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg
    13321 gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc
    13381 ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc
    13441 gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt
    13501 atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg
    13561 cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga
    13621 atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc
    13681 tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac
    13741 acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt
    13801 ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt
    13861 caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc
    13921 aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc
    13981 caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt
    14041 tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt
    14101 tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg
    14161 gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt
    14221 tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat
    14281 tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg
    14341 tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg
    14401 aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg
    14461 ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat
    14521 cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc
    14581 ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc
    14641 atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt
    14701 cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc
    14761 tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac
    14821 accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag
    14881 ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt
    14941 cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc
    15001 ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc
    15061 cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat
    15121 tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac
    15181 tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct
    15241 tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag
    15301 atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc
    15361 agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg
    15421 atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt
    15481 aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc
    15541 tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc
    15601 aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt
    15661 ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg
    15721 ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc
    15781 accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata
    15841 gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat
    15901 catttcacta ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg
    15961 atgtgccatc ttattcattc cctttgacta atattgacct taattataac gaatttgata
    16021 atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat
    16081 acggaatata ctattccgta tc
//
LOCUS       DAFGFZ010000065        15692 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000065 DAFGFZ010000000
VERSION     DAFGFZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.065"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            32..2050
                     /locus_tag="LR726_001803"
     CDS             32..2050
                     /locus_tag="LR726_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL6216894.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2120..2326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2477..4891
                     /locus_tag="LR726_001804"
     CDS             2477..4891
                     /locus_tag="LR726_001804"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL6216895.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5239..6792
                     /locus_tag="LR726_001805"
     CDS             5239..6792
                     /locus_tag="LR726_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL6216896.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7173..8957)
                     /locus_tag="LR726_001806"
     CDS             complement(7173..8957)
                     /locus_tag="LR726_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216897.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8972..10693)
                     /locus_tag="LR726_001807"
     CDS             complement(8972..10693)
                     /locus_tag="LR726_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6216898.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10793..11554
                     /locus_tag="LR726_001808"
     CDS             10793..11554
                     /locus_tag="LR726_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL6216899.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            12521..12789
                     /locus_tag="LR726_001809"
                     /pseudo
     CDS             12521..12789
                     /locus_tag="LR726_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            13461..>15692
                     /locus_tag="LR726_001810"
     CDS             13461..>15692
                     /locus_tag="LR726_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216900.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         5189 a         2739 c         3226 g         4538 t
ORIGIN      
        1 taatagataa attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag
       61 acgtttagaa cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac
      121 tgcgatggta ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga
      181 tgaaacacct gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc
      241 tacttatgct caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca
      301 agcagttgta gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct
      361 ttttgggata aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt
      421 aaataatcaa tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc
      481 tttcaatgac aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc
      541 cggtacttgg aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg
      601 ttatgcgact gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt
      661 gacccaatat gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa
      721 taatactgga agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg
      781 ttcagcaact tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa
      841 taaattcggt atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat
      901 ttatccagga caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa
      961 ttccggtaac aataccaact cttcaggaaa tgctggtaca tcaaacggag gacaaacaac
     1021 tggtgcgaaa tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg
     1081 aattacgatg gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg
     1141 acaacagcta atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa
     1201 tactgggaat acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg
     1261 aacgaaatat acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat
     1321 tacgatggct cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca
     1381 acaactgact gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc
     1441 aaccaataca aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta
     1501 taccgtgaaa gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga
     1561 tcagttaatc gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat
     1621 cgttaaaggt ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc
     1681 aaatacaccg aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac
     1741 cgtgaaagcg ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca
     1801 gttaatcgaa tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt
     1861 gaagaaaggt tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac
     1921 tgtgaaatct ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca
     1981 actaatcgaa tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt
     2041 agctaaataa aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac
     2101 tataaggcta cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc
     2161 agagagcgca tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc
     2221 cttgctgaat acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt
     2281 tcactaaggt aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt
     2341 ttataggatt tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa
     2401 ataaacagat tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag
     2461 aacaggagga cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata
     2521 ctgggcaaaa acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc
     2581 actggatatg tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta
     2641 tacggcaacg gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc
     2701 aatggggtgg gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga
     2761 tccagctgag tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg
     2821 atttagttat gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac
     2881 acaatggatc tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt
     2941 aaactgggta cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag
     3001 tgaacgtggt ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac
     3061 tgcttatgct gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa
     3121 agaaatgcaa agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc
     3181 tggtacggat aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac
     3241 ttactcggtt cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa
     3301 agctgctgta gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga
     3361 tcttgcaaaa gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg
     3421 caaggaaatc ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat
     3481 catggctgtt ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga
     3541 aattcttcca gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac
     3601 gcacatcaac tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa
     3661 tgaatggcta gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg
     3721 gctattctcc cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg
     3781 aacagtcaca gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat
     3841 caagccaagc ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt
     3901 tgatcctgta acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg
     3961 aagctcatgg tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta
     4021 tgaaaagttg aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt
     4081 acttcattta ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc
     4141 tacaaaagaa ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga
     4201 aaaaatgtca aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg
     4261 tgcagataca ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg
     4321 gagcgaaaat ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt
     4381 tgatgagaac aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa
     4441 agtgtatcat caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac
     4501 agcaatctcc caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta
     4561 tgaatatatc gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga
     4621 actatgggca atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga
     4681 tgaagcagca cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg
     4741 tgcgaaaagc aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc
     4801 caatgaaata gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc
     4861 aaacaagtta gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag
     4921 ttgtgcaaaa tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt
     4981 tcaaattgat taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca
     5041 atgtcttttt cttctgaatg gtcttgtaag agatctctgt aatcatcaaa tttttgtcta
     5101 ttaatcttag tattttttga tgtataaact acctgaaatt gagagtacat accaaattca
     5161 taaatttttt attagtggga ctagtgattg tatggatttt gacgcataat gaatgaagta
     5221 tgagaaaagg agagacaaat ggatcaagaa ctatttatta tcgaagtatt atcagctaaa
     5281 caaaaaatta tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc
     5341 ttagtatttt tctctggttt gctttttttc ggagctgtat cgttcctata tcgccatatg
     5401 agtgcagtag tccgttcagc tgaagggatg caaaaattaa ataatttgta tcacttttcg
     5461 tggggagttg gcggaataac aattgttgtg cttattgttt ttcttctact tatttggctc
     5521 cgttaccatc aattgtcgcg tcatttcgat caatggctta gtcaaagagc acaacagttt
     5581 ttacataata aaccaacttc tcaagatgat gactatcttt attttgagaa gacttcgacg
     5641 agtcaagctt ttcggataca aaaaagtgat tgccagttat tgctgagatc acttgatgat
     5701 ttacctatct acttaggcga aagcaaatca aaattcggaa tgaacacatt ccatattttt
     5761 ttactagaaa acgaacccag atgggaaaaa agccagtctt cgcctggtgg atacctgaca
     5821 actaagatga gtacattagt cactggtttt ttcctcattc ttttagctgg aacaatctat
     5881 tatgggagat acactcagcc aaaaatgtct tctcatataa atgctgcttc tcacatgaca
     5941 atgaactcaa ctaacgatac gcatatacaa aagacaaatg aagggttgtt agctgatcct
     6001 aagcaaaagg taaatttgac aacacaaaag ataaatgatc tcaaacttga tacagagacg
     6061 cactctctct atatgacaac agatagtggt gctaattggg tatttgtgcc gattgattcg
     6121 caatggttgc gttttggcga atacaccctg acaacggggc tgattcctga aggttattgg
     6181 atggacaaca catatgatat ttccgccgat ttctcatggt ttatctattc ttctggggat
     6241 gatgtacgtc tgctgacttc aagagataat ggtaaaacat ggcaaacgtc tattatcacc
     6301 caatcagttc agccgatacg ttatcgaaaa gcggtatttt atggcaacga tggtctattt
     6361 atctgtacat cagccagtgg gatgtctgca gaaactttgt acatttacac tactatggat
     6421 ggcggaatca cttggataaa aagtggaagt acaatgatca atcagccagt acaaaatgtc
     6481 agcttcgtta caacttcttt aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac
     6541 aacggaggac gctcattcaa agaatcagtt ctgtcgattc cggaaaacta tcagacaggg
     6601 gggatagatc tttttcaatc accaaatgaa gttactcaaa taagcgcgaa tacgttagag
     6661 acaaaatttt atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc
     6721 ttccgttcaa ctgataacgg ggaaacatgg caattcgttc agcaactatc gcaagtagaa
     6781 caaggagatt aaagaagaga gattgtgagc ctgacgttca gcaccgagtc acaagaaaca
     6841 aattcaaaaa acagcttctc atatttgttg aatagtgtaa ctcgtgattc ggagttgaca
     6901 agagcaacaa gaagcgattt tgaagtatag cttctcgtat tttacaagat cgtgtcttat
     6961 tgccgatagg ttgtctcttg aacaccgttt attcgtaaaa agggattgtg acaagtcttt
     7021 tgtcactact taccgaacga aactgaagga gctatccaaa aagtatcatt agtattattc
     7081 tttgtgtttc tagtggcacc actagtacat ttaaaaaagc tatcgatcat ggactaatca
     7141 tacatgatcg atagcttttt agataatgtt tgttacgcta tctgaaattg actattataa
     7201 agactggcat aaagcgttgg tgattggagt agttcagaat gtgttccttt ttcgacgata
     7261 tcgccattct tcattacaag gatcatgtct gcattttcaa tcgttgacaa acggtgtgca
     7321 atcacaaaac ttgtcctatc ttttgtcaca gcttccattg cttgctgaat caatgcttct
     7381 gttcgtgtat ctacgctaga agttgcttca tctaaaataa cgacaggagg attcgctaga
     7441 atgactcgag caattgtcaa taattgttgc tgtccttgag ataacgtgcc gttttcgcta
     7501 gaaatgatcg tatcatatcc gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt
     7561 tcagccgctt cgataatttg ttctcttgtg gcatccattt tcccgtaggc gatattatca
     7621 gctaccgttc cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt
     7681 tctccacgtg aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc tttattcagt
     7741 tcataaaaac gcatcagcag attgactaaa gtcgtttttc ctgcaccagt tggaccaacg
     7801 atcgctaccg tttgttttgg ctttactgaa aaatcgactt gattcatcaa tattttatct
     7861 gatgtgtaac caaactgtac atttttgaaa gctatttttc ctttcggttg ttctaaaagt
     7921 cttgcatctg gaaggtctgg aatttcatct gtttcatcta atatctcaaa tatcctatcg
     7981 attgctgcca ttgcggattg gatcgagttg atcacataag aagcggtaga aattggttca
     8041 gaaatctgat tgatgtactg aagataagct tgtaagaagc cgatcgtcac accacctgat
     8101 agaacaagca tagcaccgaa taccgcactt atgatgaagg cgagttgatt aatgaaacga
     8161 atggccggat agatagcaca gttcataaat tgagcacgtt taaaagcgtt gtattgcttt
     8221 ttatttactt cgtgtatagc agcttctgca tgagcttgct gattgaatgt tttgaccact
     8281 agattaccag ctaagatttc ttccattcga ttatttaaaa cacccagttc atactgattc
     8341 tgttcagaaa tctgtttgtt tttgttcgca atcttagttg taacaattga actgccaacg
     8401 atcagcaata agacgagcaa agtaagcttg ctttcgatat aaaacagcat cagaccagca
     8461 gcagctattg ttacgacaga agtaaaaaat tgattgatac ctgtcaataa cacttgagaa
     8521 agttgattga gaccggtagt tgtccgacta atgatgtcac ctacttgatg attgtcaaaa
     8581 aacgacatcg gtaatgtttt gaattttttt gtgatttctt ttcgcaccct caatgtgatc
     8641 ttttcgctta acgctgccat tgtccgttcc tgaataaatg aagtgatgct gcttaagact
     8701 gaacagccga tcagcaataa gacagggaac aacaatgctt gtttgatatc actgaaagaa
     8761 agctgtccaa tacctgtttt tcttatttct gataagagat catcgatccc gattcccatg
     8821 atcaagggca tggctacgac taataggttc ccgaataaac tgcatagaaa caatccgtaa
     8881 aaatatttat gttcaggttt gatcattttc caaaaacgta tcaaagcacg cgtgttcaac
     8941 gtttttttct tttccatcgt atcccttctt tctatattcc ttgtgatttt gcaaactcct
     9001 gataagtttg gcaattagac agtagttctt catgagtacc ttgtccaacg atttttcctt
     9061 catctaaaac aatgatgtta tctgcttgtt ggattgtact gaggcgttga gcaacaatca
     9121 cgagagtttt ctccgacatg tgtttatgaa gggctgctct taatgctgca tctgttttat
     9181 agtctaaagc ggagaaacta tcatcgaata catagatctc tgctggtttg atcaacgctc
     9241 gagcaataca tatccgctgt ttctggcccc ctgaataatt agaaccgcct tgagcaacaa
     9301 agctgtccaa tccgtcagga agggtgtcca ctagttcttc taattgagcg atcttgatcg
     9361 ctttagacaa ctcttctgtt gaggcatatt catttcccat ctgcaagttg cttaaaattg
     9421 tcccgctgaa taagaaagcc ttctgaggga cataactgat gtgggagcgg atctgttctt
     9481 gagtcatttg agtaagagga ataccaccaa gggttacttt tcctatcgta gggtcactaa
     9541 gtctcaagag aagtttcgcc aaactacttt ttccagatcc ggtcccgcca acgatcgctg
     9601 ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag aactggatca gagatttcag
     9661 tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag
     9721 ctactgtttt gatcgtctct tcctcaattt cgatctctgc ttgcagtatt tcttctatcc
     9781 gtcgtaatga ggcaaacgat ttagggataa tgaccaatac catagctgcg atcatcaagt
     9841 aagcaagtgt cagcatagag tattcgacta cagcagtcac gacaccgatt tgtaatgctc
     9901 cttctgctac aagatttccg ccaaaccaca aaatagaagc ataaacgatt cccatgagca
     9961 aaaaagcaat tggagtgacc caagcaaata ttcggttcac tttgatcatt tgattagcat
    10021 agtcagcaaa gctcttattc gttcgctttt cttcatatga ttgattatca aaggcacgga
    10081 tcatattaat cccagtaaag aattctctca atgtgatggt gatccgatcc atttttggct
    10141 ggatactcaa agataaaggt gcccctcgtt tcatcagtcg ataaacaatc catacataaa
    10201 aaacaacaga gatcaatgta acccatgtta gtaatagaga ataacgaaac atcatgagaa
    10261 tggtgaaaac gcaaataacc ggtgctggta agatcatttg gcaaaataaa acaatcattt
    10321 gctggacatt atcgacatca ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac
    10381 tgatatcctt cattgacagc tgttggaatt tacggaaata tttctcccgc gtttggaatc
    10441 caaacctagc ggaagccaat gctgaaaagt aactagaggc aactgcagcg attgttccta
    10501 gaaacgctac aagcagcatt tcaactccaa ttttttttat aactgaggct tcgccacttg
    10561 ctatacctgt gtcgatcagc tttgctacta attttggtac acctaatgta ccggcgattt
    10621 ctaaacagat catcacaaag gtaaggagag cgagtccttt gtttttctgt aagaatgttt
    10681 ttaagataga catattattc ctcctttttt cctgatactt ttcgattcta taccttcaag
    10741 taacttgaag gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa
    10801 aatgtatacg attggccaag tatcggaact atttgatttg ccaaaatcaa cgatccgcta
    10861 ttgggatgaa caaggattaa tccgttcaag tagacaagaa gagaatgatt atcgattgtt
    10921 tgatatcgat gatattttta tgctttatga tatcgttttt tatagaaaat tagatatacc
    10981 gatcaaacag atgaagaatt tatatggaaa aacattgacg gagttatatg agacacttga
    11041 tgaaacagaa agaagaatcc ataaagaact ggtggtgatg aaacaaaaac aaaaagagat
    11101 aagggagcaa aaaaaacagt tgaaattgat gatcgataca aacgaggaag aatttcctgt
    11161 agaagagatc ccttttgact gtatgatctc aacggagttt gaagatattg tggaaatcaa
    11221 aaaatttcta cctaactatt caagctttgg gatgatgagt atgccagctt ccagttcaca
    11281 gacgatgtat ggatttttta tcgaccctag tgaagtacat ttatttactc aagacgtgat
    11341 ttgggagaaa aaagatacgg ctgtttatcg tcgattctta ttgaaatccg agatgaacca
    11401 tgcggaacgt aataatattt tagaaatcag agaaagaatg tatgaaaaag gatggaaaac
    11461 aggagaagtg atcggccagt atctattgac gaatacggat gaaaacaata tcagaactga
    11521 atattatcat gcatggatag aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa
    11581 aatgatggtt gttttagtta taaagaaaaa ttttctggtt cgattacggg tttaatgtgg
    11641 gggattatta gtcgtggata gatagaattt tagtttttta gaggaaatat gtctttaaaa
    11701 atggaatggt tgcaattctt agcgatatat acgatgtaat tgtcctctta aaaatgtatg
    11761 gaaaaataat tgtattgggt gttatgtata attcatggtg aactgagact aatgagaaga
    11821 aaaattcata ttgttatgag tgagattgta tcagatgaga atgataaatt aaataggtgc
    11881 ataaaagcat tacacttaaa atacattgtt gcttacatta ttacaatatg ttaataaggc
    11941 aaaaataaat agatggattg aataaacata ttttttaatc aaatattccc tactgtgact
    12001 ttcctcagct atcggtgata ctaccgatag catttttttg cttataaaat aattataagt
    12061 atttagagat ttttcttcta acattcttca aacaagaaaa atcacaaagg ttattgttat
    12121 ttttatatat tctttttaag aatgattgtt tttttataga aattttaaaa aataaaacac
    12181 gttattgatg gatagttagt ttgttatttg gtaatattat agaagttgac tggtagtgat
    12241 ttatttttcg tcgttttata aaaatgttat tttttggaaa aattcacaaa tatgtaacga
    12301 atgtttttct ggaatttgct gtgttaaaaa gcagtatgac taatataaat actattgtca
    12361 tttatgcaat tttttatgga tagtaatttt agaggggaat caatgaagga agaatagaag
    12421 ggaaaaatag ttgcaaatac gccagttagc aaaaatagca atacatcttc aagctttaaa
    12481 attcgagaga atcatcttgg tagtggaatc aactacaaca aaaggattaa accaattaat
    12541 agatagaatt accaatggaa aaatagaaaa ggtagttatt ctatacaaaa gttgtttaat
    12601 tcgcttcggt tatgaactta gtgaaaccct ttgcaatata tggtacagaa tatagaaatt
    12661 attgacatta cagaaaatga tgaagattta atacagattg tcactatttg tagatacctt
    12721 ttacaaggaa accgagccaa taaagctaag aagatgatta aggagttgat ggaagacgat
    12781 acaagttaag aaagtgaaac tacgcccaaa taaagagcaa aaagagtaat tggacgtgga
    12841 aaatgcaaga aatgaaccat cgattcggtg gacagtttat tagtaatgac ggtttgagaa
    12901 catccatgac gagaatgaag gaacatccta aatgcgttta gttgaaccaa gtgccaaata
    12961 atatggccaa ataagcgatc agttatcgat gaatcccaag tattctaaac tatatattca
    13021 ttttgatgct aagcattgat atattttttt gggattgatg aaggttaacc aatacaggga
    13081 ctcactgatg aaattctaga gtctgacctt ggtatcaacg atttagtgat tgtttctaat
    13141 ggaaaagtat aaaagtatat aagtacatta acaaaataac tattagggtt acgctccagc
    13201 ttgtgcaagg cactggcagg aggaatcgtg aaatcataat tatggttaag ccacacggac
    13261 aactaaaagt catacaaagc tatcgaatca atcaacagaa gacaatcggc aatcattagc
    13321 tttgtttaca gcttacattg caacgcttga gttgaaccga aaatacgagg gaggtaacgc
    13381 agccattttg agttggggaa tctttttaga aaattatcgg gtaccttgtt gtttagtcaa
    13441 aattataagg gggagtaata gtgaggaaca aatttcataa actgttaagt atgtttctag
    13501 ttatgcttat tgtgatcaat caatttggga acataacggt tctagctgag tctttagcag
    13561 aacagccaga gcgttcgctg acgatcgaag ttgatcaaga ggaggctgct ttgggtgagc
    13621 agttccagtt gcgagtcaga ggaacaccag acctgctaaa tgcattggtc attgataaga
    13681 gtgaggattt gattgaaggg gaagtgaagc aactcaatga ttacgaacgt ctgattactt
    13741 attcttccca gcattcaggt aaatacgaat tgaccgcaag aagcggtgat attcaatcaa
    13801 atacggtctc aatagaaatc agtgtaccag aagaaagcac aagtgagtct tttaatgtac
    13861 cagaagaaag cacaagtgaa tcttttaata taccagaaga aagcttaagt gaatctttta
    13921 atgaagccag atcatctgag ccatctataa cgcaggaatc tcagattgaa agacaagagg
    13981 agctcttgaa ttatgacatc ggcatctctg ctgagcgccc cactttgccg agcaggcata
    14041 cacaaacgtt aaagggatac attcaaggag gcgctggcga tattcttgaa gaatatgggg
    14101 agatggtcgt acgagttcct agagcatcga ttggcttaat tgagttcatt agagtggagg
    14161 tcgctgatga ttcaccttta aagccactgg cattttacca agaaggcaca gacgttgttt
    14221 ttccatttac ctttgattat gaaggattaa atgaagccgc tcagtcgtat acattcagta
    14281 tcacgtttga agttgctttc tttcaatcag gcggtactga gccaaataat gactttcaag
    14341 tgagtcttac ttatgctgag cgagcagcaa acacggaatt ttcaattacg cctattcctt
    14401 catcaagacc cattttttcg aaattatact atggctctag agaaatcgac agccaaggaa
    14461 atcttgggaa tctgctgatt gacgggcaag acgtgaccaa cagtaataaa tttattttgc
    14521 aggtaaatta tgaaggatca cgaaaagacg gtgtagtggt taccgatgta ctaccgcaag
    14581 gaacaacttt ggcaccgaat ccgactacat ggagttccac tggaaataga gaacctattt
    14641 caggtggggt tcggatcgca gaagtaacga atccaggtga agcaaacgtt agaactcatt
    14701 atgtttccga ggaatttgtg gacaaagtat cttttaataa ggaaacgaat acacttgttg
    14761 tggactttgg taatctagca gagggtcaat cgtattttat tgaatatgct cttgaagtga
    14821 ccgatgtcga cttgttcaat gaaggaagac aagaggatgg gttttctcgg aatcgagcga
    14881 gtatgaccta caataatcag gtaatgagcc gagactaccg cttgagaatc cctgaaagta
    14941 atcttccggc aatctcttta agaaaaagtg tggacaaaaa tgttatcaat ctcaatgaaa
    15001 atgaactaac gtatactctg catgtctcga tgttttctgg aacaagaaat acgcttgtta
    15061 ttgaagatgt tttggaagaa ggcatggaat ttgtgtcatt tctatcagat attccctctt
    15121 tcattgatgc cgaagtcaat aatggaatag ttcgtttcag tgcgaataat acgattggtg
    15181 gtccgacaag ttttaccctc tcttttaagg tcaatgtaga ggcgtatgcg gtcggcgatc
    15241 aaattttaaa ctttgcaacg cttgtgaata gtgacaatcg attgaatacg agtactgtaa
    15301 gaacaaggaa aatcgatggg cgtattaaag taattaaaac tgatgagaat ggccaaaggc
    15361 tggcaggagc aaaatttgaa attttatcaa gcgattcaca agtgatacaa gaaggtataa
    15421 cggcagaaaa cggagaattt ttaagtgaac cgcttcctgt aggtagctat caagtccgag
    15481 aaacagaagc accggaaggg tatcttttag atgcgacggt acatgatgtt tttataaatg
    15541 aatactccat agaagaagtg acgttaacca ttgaaaacat ccttgatacg ggtggtgttg
    15601 aactaattaa acatgctgaa ggcagtggtg aagtccttca aggagctgtt tttgagttac
    15661 agaaccgaga aggagaaacg ctacaaaccg gt
//
LOCUS       DAFGFZ010000066        15674 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000066 DAFGFZ010000000
VERSION     DAFGFZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.066"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            31..261
                     /locus_tag="LR726_001811"
     CDS             31..261
                     /locus_tag="LR726_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL6216901.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(352..717)
                     /locus_tag="LR726_001812"
     CDS             complement(352..717)
                     /locus_tag="LR726_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216902.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            925..1407
                     /locus_tag="LR726_001813"
     CDS             925..1407
                     /locus_tag="LR726_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216903.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1604..2494
                     /locus_tag="LR726_001814"
     CDS             1604..2494
                     /locus_tag="LR726_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL6216904.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2819..5293
                     /locus_tag="LR726_001815"
     CDS             2819..5293
                     /locus_tag="LR726_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL6216905.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5396..7132)
                     /locus_tag="LR726_001816"
     CDS             complement(5396..7132)
                     /locus_tag="LR726_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL6216906.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7129..7833)
                     /locus_tag="LR726_001817"
     CDS             complement(7129..7833)
                     /locus_tag="LR726_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6216907.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7964..8466
                     /locus_tag="LR726_001818"
                     /pseudo
     CDS             7964..8466
                     /locus_tag="LR726_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            8426..8764
                     /locus_tag="LR726_001819"
     CDS             8426..8764
                     /locus_tag="LR726_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL6216908.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8785..10203
                     /gene="ffh"
                     /locus_tag="LR726_001820"
     CDS             8785..10203
                     /gene="ffh"
                     /locus_tag="LR726_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL6216909.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10315..10893
                     /locus_tag="LR726_001821"
     CDS             10315..10893
                     /locus_tag="LR726_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL6216910.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10897..11418
                     /locus_tag="LR726_001822"
     CDS             10897..11418
                     /locus_tag="LR726_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL6216911.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11497..12051)
                     /locus_tag="LR726_001823"
     CDS             complement(11497..12051)
                     /locus_tag="LR726_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL6216912.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12304..12579
                     /gene="rpsP"
                     /locus_tag="LR726_001824"
     CDS             12304..12579
                     /gene="rpsP"
                     /locus_tag="LR726_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL6216913.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12591..12842
                     /locus_tag="LR726_001825"
     CDS             12591..12842
                     /locus_tag="LR726_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL6216914.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12967..13758
                     /locus_tag="LR726_001826"
     CDS             12967..13758
                     /locus_tag="LR726_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216915.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13928..14449
                     /gene="rimM"
                     /locus_tag="LR726_001827"
     CDS             13928..14449
                     /gene="rimM"
                     /locus_tag="LR726_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL6216916.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14439..15200
                     /gene="trmD"
                     /locus_tag="LR726_001828"
     CDS             14439..15200
                     /gene="trmD"
                     /locus_tag="LR726_001828"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL6216917.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15336..>15674
                     /gene="rplS"
                     /locus_tag="LR726_001829"
     CDS             15336..>15674
                     /gene="rplS"
                     /locus_tag="LR726_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL6216918.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEI"
BASE COUNT         5222 a         2812 c         3220 g         4420 t
ORIGIN      
        1 tggatttttg ataggagtag aaatgcaagt ataggtttta gaactaaacg aagtctgtct
       61 tctaaaaaaa attgggtata ctcacaaact attttttatg gaggaattgt tttaattagt
      121 ttactttctt caacacttta ttctttgaat attatagatg tttccacctc aaattcaata
      181 tctattattg gaatcattat tgctgcaatt attacacaac tgtttttagt gtttggagag
      241 aagaaaagaa gtaaaaaata gaaaaactag tgatagtttt ggtgatataa gaaaaacata
      301 tatgtataaa aagaagaaag tagcttctct ttgtacatat atggtttgtt tttatggatg
      361 ccgagctttt ttataagata aggctaccgc ccataacgac ccaaggattt gtggaactaa
      421 gtaaatacct gcaagaagca agatcagctg ggtcggtttt aaaggatgat agaagtaacg
      481 ccattcagta aaattggcgc aaaataaata ggaagtgaca gtagatataa tattggcaat
      541 gaataaagat ataatacgcc tgttcctttg aaaataatat ccagcaaaaa gagataaaaa
      601 tatcaatgtg atgaacccgc ttacggaatg ataacgaaaa tcaagcagta aggcgaaatg
      661 cacataagga acaaaagata aaagcaacgg aaggtttttc cagcaagttt ttatcatctt
      721 tcatcaccac ctaacaagtg ttattatttt gttagtttta ttgtaaattg aatcctgcaa
      781 taatcaatta ctttttcatt atttctagat taaatgaata aactctcatg ccattatctc
      841 taaaaaaaaa gtttttcccc ctaatttttt tagatagaaa attgtatagt atgatgagag
      901 aaaaagtagt ttgggggaac gttaatgaga agaaaagcag taatcgatcg gctttattta
      961 tttttttggt tttttcctat cgttttgcta ccttttacat gggtaaatgc tgttgtacaa
     1021 ggtgcggaga tattaggcct gatccaagga agcggattag tggtttctag gaattttcca
     1081 gctttgactg ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc
     1141 aatcactaca attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt
     1201 tctttgctgc ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat
     1261 tcagcttatt cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc
     1321 tcatttattc ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga
     1381 tctgtatcta taaaaaataa ctattgatgg tggatcatca gtaaaaaagc atatgagctg
     1441 cgtaaaatgc atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt
     1501 ttacatgatt tttacataac ttaactatat ttttacagca accatacgag ggatttacag
     1561 tcgactgata aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt
     1621 tactctaggt atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga
     1681 tggctctgcc acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt
     1741 aggttcgaca gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa
     1801 tccaaattat caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga
     1861 agctggagca gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga
     1921 tgcttcaaaa ttagtggatc ataaagtagc agtggtcggg atggcaccaa tcgtcaataa
     1981 agatacagat gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat
     2041 cactaactgg aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa
     2101 cggcagcggt acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca
     2161 gtcccaagaa caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc
     2221 aatcagctat ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg
     2281 tgtagaacca aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca
     2341 tatgtacacg aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac
     2401 agaagaaatc caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga
     2461 agtagaacgt gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt
     2521 cgttactatt aaggaaaaat caactatgtt tccccacata tacatttttc ctttaattgt
     2581 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
     2641 acaccgttta ttcgaaaaaa agagctacga catagaactg tcgtagcttt tttttgctat
     2701 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
     2761 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
     2821 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
     2881 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     2941 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     3001 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     3061 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     3121 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     3181 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     3241 tgtaatagat gacattgtta ttttagagac tggacatcaa attccagcag atgcagaggt
     3301 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     3361 caaagaaata ggcgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     3421 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     3481 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     3541 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     3601 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     3661 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     3721 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     3781 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaag tgatcgtctc
     3841 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     3901 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     3961 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     4021 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     4081 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     4141 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     4201 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     4261 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     4321 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     4381 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     4441 aaagaaaatc ttcgttcaat tgctaaagcg tcgtaacaat acagtagcta tgacaggtga
     4501 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     4561 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     4621 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     4681 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     4741 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     4801 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     4861 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     4921 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     4981 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     5041 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     5101 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     5161 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     5221 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa
     5281 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     5341 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     5401 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     5461 taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     5521 aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     5581 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     5641 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt
     5701 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     5761 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     5821 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     5881 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     5941 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     6001 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     6061 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     6121 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     6181 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     6241 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     6301 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     6361 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat
     6421 acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat
     6481 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     6541 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     6601 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     6661 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     6721 tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc
     6781 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     6841 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     6901 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     6961 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     7021 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     7081 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgttttgg
     7141 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttggat gttttggatc
     7201 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     7261 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     7321 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     7381 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     7441 ctcagcttca tcttttgatt gcttacgagg ttcaatccgt ctgaatattg ctttcatacg
     7501 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     7561 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     7621 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     7681 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     7741 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     7801 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     7861 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     7921 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     7981 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     8041 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     8101 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     8161 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     8221 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     8281 cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaatggt
     8341 gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa
     8401 tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct
     8461 ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta
     8521 tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt
     8581 atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct
     8641 tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga
     8701 taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga
     8761 ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag
     8821 gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg
     8881 cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt
     8941 actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca
     9001 caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca
     9061 gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca
     9121 ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt
     9181 ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta
     9241 ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc
     9301 gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg
     9361 gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg
     9421 acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac
     9481 gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat
     9541 ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa
     9601 ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct
     9661 agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac
     9721 gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat
     9781 gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa
     9841 atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat
     9901 gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat
     9961 ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa
    10021 gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa
    10081 ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta
    10141 ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa
    10201 taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat
    10261 cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga
    10321 tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt
    10381 cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga
    10441 ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc
    10501 gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa
    10561 ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg
    10621 aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa
    10681 atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat
    10741 gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat
    10801 gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa
    10861 gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt
    10921 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
    10981 actccaacag gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc
    11041 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
    11101 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
    11161 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
    11221 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
    11281 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
    11341 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
    11401 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
    11461 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
    11521 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
    11581 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
    11641 cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc
    11701 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
    11761 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
    11821 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
    11881 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
    11941 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
    12001 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
    12061 cctccttcaa actatctata ataatcttac aaaagataag taactggagc aaataaaaac
    12121 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
    12181 aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
    12241 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
    12301 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
    12361 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
    12421 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
    12481 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
    12541 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
    12601 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
    12661 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
    12721 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
    12781 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
    12841 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga
    12901 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
    12961 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
    13021 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
    13081 agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca
    13141 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
    13201 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
    13261 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
    13321 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
    13381 acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt
    13441 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
    13501 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
    13561 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
    13621 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
    13681 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
    13741 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
    13801 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
    13861 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
    13921 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
    13981 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
    14041 gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
    14101 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
    14161 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
    14221 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
    14281 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
    14341 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
    14401 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
    14461 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
    14521 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
    14581 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
    14641 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
    14701 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
    14761 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
    14821 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
    14881 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
    14941 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
    15001 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
    15061 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
    15121 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
    15181 cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt
    15241 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag
    15301 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
    15361 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
    15421 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
    15481 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
    15541 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
    15601 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
    15661 gtatcaaaga aatc
//
LOCUS       DAFGFZ010000067        15200 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000067 DAFGFZ010000000
VERSION     DAFGFZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.067"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(366..1484)
                     /locus_tag="LR726_001830"
     CDS             complement(366..1484)
                     /locus_tag="LR726_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216919.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="LR726_001831"
     CDS             1955..3262
                     /locus_tag="LR726_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL6216920.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="LR726_001832"
     CDS             3381..4580
                     /locus_tag="LR726_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL6216921.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="LR726_001833"
     CDS             complement(4607..5875)
                     /locus_tag="LR726_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL6216922.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6038..6684)
                     /locus_tag="LR726_001834"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="LR726_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="LR726_001835"
     CDS             complement(7092..7595)
                     /locus_tag="LR726_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL6216923.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="LR726_001836"
     CDS             complement(7718..8482)
                     /locus_tag="LR726_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL6216924.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="LR726_001837"
     CDS             8590..8865
                     /locus_tag="LR726_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL6216925.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="LR726_001838"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="LR726_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL6216926.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10632)
                     /locus_tag="LR726_001839"
     CDS             complement(10432..10632)
                     /locus_tag="LR726_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216927.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11437..12396
                     /locus_tag="LR726_001840"
     CDS             11437..12396
                     /locus_tag="LR726_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBL6216928.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(12482..13576)
                     /locus_tag="LR726_001841"
     CDS             complement(12482..13576)
                     /locus_tag="LR726_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL6216929.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            13765..14526
                     /locus_tag="LR726_001842"
     CDS             13765..14526
                     /locus_tag="LR726_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL6216930.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(14641..15081)
                     /gene="fabZ"
                     /locus_tag="LR726_001843"
     CDS             complement(14641..15081)
                     /gene="fabZ"
                     /locus_tag="LR726_001843"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL6216931.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         4680 a         3131 c         2658 g         4731 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaataa
    11341 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
    11401 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
    11461 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
    11521 aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggt
    11581 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
    11641 gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg
    11701 acgcctgacg tcattatcgg gcgaaaagaa aggcctgttt catgcggtat gagaacactt
    11761 tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga
    11821 aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat
    11881 cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc
    11941 gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc taaagtcatt
    12001 atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct taatcaatgg
    12061 ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg aaaagaattt
    12121 tctaactgga aagccattag taaccaacat gatattgata tatattttgc ggaccctgga
    12181 acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg taatggactg
    12241 ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt cagcaatcaa
    12301 cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat atttttgagc
    12361 tatgtacaag aagcatttta ttctagctta atttgacaaa tcataaccta taaaaaaaca
    12421 aaataaaaaa gcagaaataa ctggctattt ctagcggatt atttctgcaa taactaatta
    12481 tctatttttt attttcaatt aatttaaagc ttgaccatgg tttcaaaaag tcaaggagga
    12541 ataattcgtc atcggagatt cttcctacaa cattgacacg tccgtcgttt ttcatatcct
    12601 tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc ttcattgtca atcagtacat
    12661 ccccatgatg catatctcgc gtattatgag gcgggaaatc tttatctttg tagtatacac
    12721 gagtcatcgt tgaacgaagg atatattctg agcgatcccc tcgataacta tgaaggttat
    12781 caaacaagca gattcgttcg ttttctgtaa taccttcttc cgtatctact ttcaatgttg
    12841 gataatccgc gttaaatgct tctgccattt ctttcaattc ttcttcactt gcgtacgcat
    12901 ttccaataga aatatcatca atcaatcctg taaggattaa atgcttcact tgtgtcgcaa
    12961 tttcaagatc tcggtggtct tccagcgtgc acaagccatc ttgcgtaggc caagggccaa
    13021 actcagcaga ttgcgaattg acgaatgcca tcgtattcaa attatatttt ctgaattttt
    13081 ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta tcgatgagga taaaaattat
    13141 gagaacctaa taaattatcc gtgtttggcg aataactcat gatgttgtca acgtaattcg
    13201 tgcccgaact catattgatc tcgattttga taccaaaagg attgcgcgtc atttttgctt
    13261 cttctgctcc tgtgaatccg atatctaagc ggaccccgta ggcacccatc ttatggaaaa
    13321 aagataagtc atcgtaagaa atctccaact gttcaaataa cgctggattg atatcaacca
    13381 ttacttccat gcctaggcta ttcgcgtaat caactacttc tttaaattct gaaagaactt
    13441 tttctttatc atcattgatc tgcagcaaac tggtaaaaac ccgtttatac ccatacttat
    13501 gagctaaatc caagtacgct ttgtcctttt caaatgttga ccgttctgga taaatcgaga
    13561 ttcctaattt tcccatatta ccatttcctc ctgcatcttt tcgtttttgt atccacttac
    13621 attatagcaa acacaaagta aatggtctac accttttctt tttcttttaa aataaatgat
    13681 aaattccaat aatttaagta ggtattttcc ctctattttg ttatcattaa agataacaaa
    13741 atagtaaatt ggaaaggaac agttatgaag aaaaataaag ttattttaag tattagttta
    13801 gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca acacgacttc ttctagttcg
    13861 tctacagaaa gttcaaaaag tacatctact gcggaaagca cgactgccag tagcgtcgat
    13921 ttgaacgcat tggaattacc acaattgacg aaagatgtcg ccgaaaatga agatctggtc
    13981 gaaatggtga cgaataaagg cacaatcaaa atcaaattat ttcccgaaca cgcaccaaaa
    14041 gcggttgaaa actttatgac acatgcaaaa gacggttact acaacggact gactttccat
    14101 cgcgtcatca aagatttcat gatccaaggt ggcgatccta aaggcgatgg cactggaggg
    14161 gaaagcattt ggggagaagg atttgaaaca gagatcaaca accaccttta taatataaga
    14221 ggtgcgttat ctatggctcg ctcacaagat cctaacagta acggcagcca gttctttatc
    14281 gttcaaaata cagatgatat gcatgatggt cttttaaaag acgactatcc tcaagcaatc
    14341 atcgacgctt ataaaaacgg cggttatcct tcactagatg gccattatac tgtcttcggc
    14401 caagtgattg aaggaatgga tgtcgtggat gctattgcaa atatcgaaac agatagttca
    14461 gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata tactgcaaga agcaaaacaa
    14521 aaataaaaaa ataacgtctg aatcctgcat agatgacaac gtatgctagg attcaggcgt
    14581 cttcttttta cataaaaagc aagtaagctt ccttttgttt tgctatatca ggttttatag
    14641 tcaagcattt tggttgacga tgaacgtgaa ctcgcactca cagacttttt tatcatctac
    14701 atatgctgca gataacgctg taccgactga tcccttcatt ttggtgattt caaaatgaca
    14761 tttcagcaca tctcctggaa caactttttg acggaatttg gccttattga tcccaccgat
    14821 ataagctctt tttcctagaa actcttctga ttttaggata agtatcgaac ctgcttgtgc
    14881 cagtgtttct agaatcaacg cacccggcat cactggattg tcaggaaagt gaccttggaa
    14941 aaaatcttca ttgattgtta cattttttgt cgcaacgatc ttttttcctg gttctaaagt
    15001 atctacataa tcaatataac aaataggata acgattagga atcaattcca ttacttcagt
    15061 agcagataat acgtaagaca taattaaact ccttctttca ggcaattgcc attttcatta
    15121 ctttgactat caaagtatca tattcctaaa tatttagcaa tcttttttta atttaagaaa
    15181 aaatagtttg acaatcaaaa
//
LOCUS       DAFGFZ010000068        14734 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000068 DAFGFZ010000000
VERSION     DAFGFZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.069"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..1079)
                     /locus_tag="LR726_001844"
     CDS             complement(<1..1079)
                     /locus_tag="LR726_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL6216932.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLILLIKEFRKA"
     gene            complement(1069..1932)
                     /locus_tag="LR726_001845"
     CDS             complement(1069..1932)
                     /locus_tag="LR726_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL6216933.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            complement(2001..3836)
                     /locus_tag="LR726_001846"
     CDS             complement(2001..3836)
                     /locus_tag="LR726_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL6216934.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(3885..6275)
                     /locus_tag="LR726_001847"
     CDS             complement(3885..6275)
                     /locus_tag="LR726_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBL6216935.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(6545..7792)
                     /locus_tag="LR726_001848"
     CDS             complement(6545..7792)
                     /locus_tag="LR726_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL6216936.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            8185..8601
                     /locus_tag="LR726_001849"
     CDS             8185..8601
                     /locus_tag="LR726_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216937.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            complement(8618..9355)
                     /locus_tag="LR726_001850"
     CDS             complement(8618..9355)
                     /locus_tag="LR726_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216938.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(9773..10336)
                     /locus_tag="LR726_001851"
     CDS             complement(9773..10336)
                     /locus_tag="LR726_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216939.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(10323..10850)
                     /locus_tag="LR726_001852"
     CDS             complement(10323..10850)
                     /locus_tag="LR726_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216940.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(10875..12206)
                     /locus_tag="LR726_001853"
     CDS             complement(10875..12206)
                     /locus_tag="LR726_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL6216941.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            12332..12808
                     /locus_tag="LR726_001854"
     CDS             12332..12808
                     /locus_tag="LR726_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216942.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(12923..13155)
                     /locus_tag="LR726_001855"
                     /pseudo
     CDS             complement(12923..13155)
                     /locus_tag="LR726_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13158..13475)
                     /locus_tag="LR726_001856"
     CDS             complement(13158..13475)
                     /locus_tag="LR726_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216943.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(13490..14321)
                     /locus_tag="LR726_001857"
                     /pseudo
     CDS             complement(13490..14321)
                     /locus_tag="LR726_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14374..14703)
                     /locus_tag="LR726_001858"
     CDS             complement(14374..14703)
                     /locus_tag="LR726_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216944.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
BASE COUNT         4181 a         2982 c         2140 g         5431 t
ORIGIN      
        1 tatgcttttc gaaactcttt gattaaaaga ataagaacat ttagacttgc taatgctcct
       61 attacagtcc aaatgactaa aagcattttc tgatgtttat tggtaattcc tgtgaatatt
      121 gaacttgcct tttcagcaaa tgttctgtct tgcatctcta cgctaccttg ttccggaata
      181 ttaagatcat tatttccatc taattccttg ttggcacgat caacatcctc aaataaatca
      241 ttagaagatg taacagtttc tgtcttatct gaaacgtact cgctatcaat ggcatcagcg
      301 tatgcggtat tgccaactgt tacttgtggc gtgatgtatt ttgctttttc atctcttaga
      361 cttctaacta ataaacgatc gtagttctga ggatatggat gacaagtata aagtgttaac
      421 atatctaaat caggaatatc agtcagtgtt gaattctgac cgtaatttct aataacgtct
      481 gttcggaaaa ccgtataaac tgctttttca ccaagataat ctatataaat gtggtctccg
      541 gctactaatt gatcaacata aagaaattgt ggtgacgtat aaccgccacg atgacctgct
      601 attactgttt gatgaccttt acctgataaa ggtagactag tagcactcat ctgtgccaca
      661 cctctatata aatgttcctg tgaagcgcct agaaacaaaa gctctagcac atttattttt
      721 ggaatataga cataaccaac cgcccctgaa taggtagatg gttcaaaatc tttcgtttga
      781 ttttttgaaa aaacatctcc tgtgtaagga ttgacctttc cttcagcttc accaatatca
      841 aatgatccgc cgtctgtttt ctgagtattg tctaatcctt ggttaccgct gttcgatttt
      901 atttgttcga ctttttttgc cacttcctct tttgaaggag tttgtttttt ctcaattttc
      961 tcttggattt ccgccacatt ctcttttgtt ttgaattgat gatagagctg atttgcagga
     1021 taaattgtta aaaccaaagt gcaaataaga gtcaaaaaaa gtagtatttt attcttcatt
     1081 tttcattcct cgtctctttg ctactatttt tttaataatg actatcacag ctattattaa
     1141 taaaacccct acaactacga taggatacca gttcatctgc gcctttacct ttaaatcttc
     1201 tttcttcttt tctttctcaa caatactttt ttggtaaggt atgctaaccc ctctaacgag
     1261 taacctatga gaattatctc caacaggtgt acatgtcata agtgttacat aatcttttcc
     1321 tgccacagga agcaaatcct caaattcggt tggtaggatt atttttatcc gatctacttg
     1381 ataagcaagc tttttccctg aaatatcaat aaaaaattta tcaccaagtt tgagcaactc
     1441 taaatctgta aaatattttt tattaggaat gcccgaatga gctgatataa cagaatggtt
     1501 tccaatccca ccagttggat aatcagttcc ggttaatatt cctgctcctg ctgatagttg
     1561 aaaatcgttt acaccatcat aaattggcaa atccacatta atctttggta taagaatttc
     1621 acccactaat tcacctaatt tatccttctg aagatttcca tttgacggaa tattgttatt
     1681 atcttcagga atcgtgactg aggtttgttc tgtgtctttt cgtttcctat taacttctga
     1741 atatctcttg aagacttctt cattctcctt ttcagaagct tcatcatatt tttccttata
     1801 ggagtttagt gtttgttgag tttggtagta ggtataagcc tgagtaatta caggatatag
     1861 acctactcct aaacatagca acatcccaat ttttaagatg atcaacttga tattcttctt
     1921 tttcatgctc atacctactt ttcacacact ttttaaaaag aaaccctaga gccaaaaaca
     1981 ctctagggaa ttttatttca ttaagctttg tgttgttctc gtttgtagac aacatatagc
     2041 cctgttcctg ccacacctgc aaccactaga aatactactg ttccaattcc acctgtacga
     2101 actgtttcag gtgttttaag gttcttgatg tctgttactt cgctagaagc atcatcattc
     2161 gataccacaa atggaacatc atttctagtt ggtgtatatc ctgaaggagc aacaacttct
     2221 tcaagagcat aatcattgat ttttttatct gtgattgttg gattaccttg tttatcaaaa
     2281 tctacatcat aggtatattg caagccttca atttggaact taccgtctga tccggaaacg
     2341 aattcagtag ctttagattt atctgcaatc caagtaacag ctttcgttgt tggatcttgt
     2401 tttaggtaag taactttatt cggatcaatt ttgttcttca ctacaaattt tgctccactc
     2461 aatgctttgc ttgtatctcc atctgtttta atgaattggt gatcaccaat gattgtataa
     2521 gcgtgatcaa ctggtgtttc gtcttcgggt tcatatccat tgttgtagat cgttgaaaca
     2581 tcattcttaa gtggtgtatc cattttagct ttactattag tatgagcgtt catcaaaatt
     2641 gtaaagaagc gatcccctgc atcaaattgt ttctgaagct tatccatacc tgattcagta
     2701 aagacatagt ttgtcgtttg tccgtcacgt tcgatactgt aatcagtatc taaagtcatt
     2761 ccttcgatca cttctgatga atcataagtt aactctgcac ctagaacatc tttgacaaca
     2821 tatttttcgt aattaccaat attaccagga attgatggtt tcaatgtata ttgaacatct
     2881 tcgccagtat caacggtttc tttatcaatt accttagtaa ttacaggtcg tttgtagttg
     2941 gtaacatcaa gaactacttt agcagtctcc ccacgtttaa cgctaaattt gaatttttta
     3001 ccatctagta aataggttgt tttattagca tctgttccca agtcggtttc taggaaataa
     3061 tagtcaccta attccaaacc atctacagtt aatttacctg tgctgtctgt tttataagtt
     3121 ccaacaacct gatcagatcc atcatctttt cgcaattcaa aaacagttcc ggcaagagcc
     3181 tttccatctt cgtctttttt atcaagctct gctgagttta attggaaata gtttttagga
     3241 tagacatgta catcataatt aaagtattga tcatctgatc cgtttagttc aggtacgtta
     3301 acaatcatag gatacgcttc ttcaactaca cctttgactg ttcctgtttc aacaatcaaa
     3361 tacaagttat ctgttaaatc gtctgtattt acttgtatag gtatgtctat attcccttgt
     3421 gaatcagtct tcattctact tacttcactg tattttttgt ttagtaataa tcgtgcttta
     3481 gcatcatcaa tatgttgacc ttttgtagta tcactatccg tccatcccaa ggatttagct
     3541 tcatctttag taagtttcac ataagagaac tcaacgcctt caagaggttt tacaccattt
     3601 ggtaaagcta tttctttccc tttgttatca atatctgaga cagggttccc atccccatta
     3661 gtatttgtta ctttatggat atgaatcgtt ccagttcctt gataattata gttttgttgt
     3721 gcatatgcta cttgcccccc tagtccaagg ctagaagaga ttgagcttgc gcccaatccc
     3781 actcctagca aagtaccaaa tagcattaaa tttgaaaaaa ttttttttga tttcatacgt
     3841 cagccttctt ttttcatatt tacttctttt ccattaacac ttttttattt tgctttgtgc
     3901 tgttctcgat aatacagtag caccatactc aatcctgcaa ttagaaatac aagaaggaag
     3961 gctgatgtac cgattccgcc tgttcttact gtttcaggta caaggtgatt aattactgta
     4021 tattcaaagg tttgttcatc gtcagcaacg gtgatgcctt ctttagacca tttccctgta
     4081 taaccattag ggttttgtgt ttcttcgatt ttgtacgttt tgttggcaac aatattaaat
     4141 gttgccaagc cattgctgtc cgttttttgt acaactttta cacctttgtc gtctgtcaaa
     4201 gtgaattctg cgtttgctaa tgctttccct tgatcatcca ctttatgaac tttgatcaca
     4261 ccttttttca aagtgttttt agcagtatat tcaaagactt gtccatcatc tttgacggta
     4321 attccttctt gtttgaatgc gcctgtgtaa cctgttggta cttttgtttc ttctaagttg
     4381 taagttttgt tcgcaataat gttgaacgta gccagaccag ttgcatccgt ctttttaact
     4441 tcttttacac ctttttcatc cgttaaggtg aattctgcat ctgctaaagg ttttccatct
     4501 tgatcgactt tatgaacttt tactacacct tttttcaaat tatttgtagc tgtatattca
     4561 aaagtttgcc catcgtcttt aacggtgata ccctcttgct taaatgcacc tgtgtaacct
     4621 actggtactc cagtttcttc taggctatac gtcttgtttg ctacaatatt gaagcttgca
     4681 agtccatttt catctgtttt ttctacttgt gttgcacctt tttcatcggt taaagtgaac
     4741 tctgcatttg ccaaaggttt tccatcttga tcgactttat gaacttttac taaacctttt
     4801 ttcaaagtgt ttgtagctgt atattcaaaa gtttgtccat catctttaac ggtaattcct
     4861 tcttgcttga atacgcctgt ataaccagtt ggtactccgg tctcttcaag gctgtaggtc
     4921 ttgtttgcta caatatcgaa gcttgcaagt ccgttttcat ctgttttttc tgcttgtgtt
     4981 tgggatgaat catccgtcaa ggtaaattca gcattagcta atggttttcc atcttggtca
     5041 acctttttaa cttttacttt acctttttta attgtgttcg tagcagtgta actaaattct
     5101 tgtccatcgg tattaacagt aataccatcc tttttaaatg aaccttgata accttcagga
     5161 acttttgttt cttctaagct gtatgttaag ccttgttgca aatcaaacgt agctaaccca
     5221 tcatctccgg ttgtcttaac agatttagca ttgttattgt cagttagagt aaactccgct
     5281 ccttttaaag catttccatc ttgatcaact ttcttaactg ttacctgacc aacagctctt
     5341 gtccatttag cactcaaaga tttagagtcc tttgtttctg aagtagtgat cataactacg
     5401 gcatcttgat acacacctcg tccatcaaaa gtaaaaggat tggttgtgct aacattagca
     5461 gtacctttaa atgacaaagt accacttggt gtagtcacag gtactttaat agtaaaactt
     5521 gaatttaatt tcacagtgtt gttagtaatt tctttaccat ctgcatcata aatcttcata
     5581 ccagctggtt ttgctgatgt aaaagtgagc ttcgcattac cagtcttatt tgatgctgtt
     5641 ttatatgtga aagtgtgata ttttccttcg tcagctgtct tagaagctgt gatagaaaac
     5701 tcagtgttgg gcgtatcttt tccattctta gcatagtcat aaattttttc aaatgctttt
     5761 tttacacgag ctgtttctgc ggcggtatgg tttggatgtg accatacaat ctgtgaaact
     5821 ttaaagtttc cggctaaaac ccatgaaact aattgtgttg cataccatgc ttctgtatca
     5881 tttcctgcac ccagttcctt tgctgttttt gatggaaaac cacctttaaa catacgatag
     5941 aactcatcac tgccagctcc cttgtacttc aagcttccgg ctccacttcc tgttggggat
     6001 ggcaattctg aatccataca atatccaata tttccatgag aatctttaaa ttgacttgaa
     6061 atcatccatt catttggatg attcggcata ttaaccctaa tataaccgtc tgtttttacg
     6121 ccagtttctg taaattttgc acttgtcggt tttgtgacat ctgcttcagc tcttattgct
     6181 gaaaaaggca ttataagcgt agataacacc attactaaag tcgcaaaaca ataaattatt
     6241 tttgatttta aaattatcgt tttttcatgc ttcatctctc gttactcctt ctttatacca
     6301 gttaggcgaa gaaaatgagc cacgatatgt cggtgtggta atgaccttcg cgcccattcc
     6361 taagttaaaa tcaccacacc tattaatcac ttttttcgtt gaaagcaagc tgtttcaacg
     6421 ctctagtatt catagcatca ccccttttag ttctaccaac catgagcaac aagttttcta
     6481 agtagttaag caaggacaaa taaagactat acttaactac ttagtatata aacgatctct
     6541 tttattaacg taccgaaacc gctaattgag atacgttata tgctgtttca aatgatcctg
     6601 cattaatcca acttgcccca aacacaggat cgacaacatg gtaaaaaccg tctttaaatc
     6661 catcaacggc tactacatgt cgattccaaa tagcatcgcc ccaaaacatt gatttatgtt
     6721 gagcaggttc aaaggcataa gttacccacg ctactacagg atttccattt acaatctcat
     6781 cttttaatcc ttgtaccgat gaaccagaaa tatctacaac atttccgcca ttagcaacac
     6841 cccatgaaac tacaggagcc ggattcatac cttgataagt tccatccaca ttgtgtctcc
     6901 attcaccaga aaaaccatta tttggattat tatcaggaga tagtggcatt gatttaacaa
     6961 aagtcattaa atcttgattt gttgcaacac ctttataatg taacgcttct agcaaagatg
     7021 ttccttcgca aagcattgtg ttaccttgac taataaatgg aacgcctagc acaacattag
     7081 ttggttgttc gggtttactt ggtgaagtag aactgtcagt ttcatcactt gaacctgttt
     7141 gtgattgatc actagaagat gtactggttg attcatttgc atcatttgat gttgattgtg
     7201 agttatttgt tgatgttcca gtatcagctt ccgtagtgct actttcactt gttccctgtg
     7261 atgcttcttt ctcacttgta gaattatttg tattagatga acttgctcca tcaacacttg
     7321 aagcacttgt ttctgtctta tcagaagtgt ttgctgttgg ttccgattct tttgtagtct
     7381 cggatttttt gttctcttgc tccgtatttt caacaggatc aggtttatct ttttgaacag
     7441 tttgactaac tacttcgcca tctgcttcct taactgttaa tgtatcttta ttgatgttga
     7501 aagttcgtcc aatcgggata gtccgttgtt ctccttttgc caaatcaaca ttgttcaatt
     7561 cggcaaactc agcaacagta gtcttgtaac ctaagctttt aagcttctct acagttgacc
     7621 aaatagtatc tccttttttg aacgcataac ttgattgtcc tgttgtaatt tggatttgat
     7681 ccggtgtgtt tgcagtccat tcatcagcat gtgaggttac tggtactgat aaccccgcaa
     7741 gtacagctga tgcaaataaa accatagcaa gcgaattttt cttcatattc attccttctt
     7801 tttgttttta ttttctgttt gtgatcgcaa tgcttctatt ccttcttccg ttataatata
     7861 ggttttgtca cccactttct tgaacgaacc tttaggcaaa cgatttggat aatagttgat
     7921 tatgtttgta acataatttc tacctttacc caaaattaga cttgcttccc ttgcggtata
     7981 caagttgtat ttgtctaggt tcaatttctt tttatttgcg atttagacct acccctttcg
     8041 caaaatttat tttataataa tagtaccatt gcactatcat aattgcaata attttttggg
     8101 gtttatcttc atttaatagc ctaaaaaggc ttattagata aggaatatct aactaatcaa
     8161 aacaaaaaag gaagttaaga agaaatgaac aaaaaaagga aattatcagt attaattaca
     8221 cttgtttcta tgttggctat tcttagtggg atcttgtcga ttaaaatttt caatcaaata
     8281 aatacaagcg caaataccaa aaacgaaata gtcatagaaa atacatctaa ttctaaagaa
     8341 attagtacta ctaaaaacaa agacaaatct gatgaaacca ctactaaaaa ttatctaata
     8401 aagactgttt attttgatga acggaaaaca gcaattctca aagatgagga aggatcagaa
     8461 ttaaagctac ctataacggt atccgataag ttcaatggaa cgttctcctt acaaaccgct
     8521 gaaggatcac ctgataatct caaagttggg caaacaatca ttcttggtaa agacgaagaa
     8581 ctgtattcat ttgtaaaata aaaaaatgga gcaaaggcta gtcaagccct tgctccattt
     8641 tccattcttc aagaggtgta gctatttcaa caccaaagtt taaatactgt ggatgctgta
     8701 gatacatgat attcattcta ttttcgataa actcttttaa cgttgagtaa atatattttt
     8761 ctaatgctat atctgcccaa ctatcataat caagtctatt ggtgatattg acgtttattt
     8821 caatttgaga aacatcaata ttttgaacat caggaaactt attcagtctt agctcttctc
     8881 taaccgcttc tactagcggt tcatattgtc cgtcttgttc gctaattgtt gaaactattt
     8941 catctgaatt ccaacgatct tccaatacct ttgctacatt ttctttaggc ggttgtcttt
     9001 ctataaattc ttcaggtgtt tcatcaaact ctactctaaa gttcaattta ttttgattgt
     9061 tagcgtacat ataatcatct ctgtttgatt caaaaaagtt acgaattcct gtatctgatt
     9121 tatcacttaa ctccttattt gctaagttag cccaaaaatc ccaagttcct ctattttcag
     9181 tatacaaagt aaaattaatg tttatataat ttgcagtttc aggataccct tgctctctaa
     9241 gagcatcttt aacaatcacc gctaagtcag tttcaaaatt ctccataaaa aattcactaa
     9301 attcttttgg atcccacgtt tcttctaata acttttctaa tttacgatct gccattatta
     9361 ttctccccca ctttactttc ttatatctaa aactgcttaa aattaagagc tataacttat
     9421 catagaaggt tttttatgat ttatttaagt ctacaattct atattctaat tatcatctag
     9481 accattttct tatgtcaaat aaaccatata tattgtcttt aatagtttta tttgactttt
     9541 taagaaagta tttacttttg acttttttta cgttttacaa catatacgta ttgtaatgtt
     9601 cagctcaatt tattataatc aaaatgaatt gattaataaa aattctgagg ttcgggtttt
     9661 ttatcaattc ttttctgttt gtgtttgtga tgaaacaatc ctttttcggt aggttttgtt
     9721 atcatcgtca gcacaaagag ctaacgttgg taccgttagc tcttttttta ttttactctg
     9781 ctcttacata gctatctaaa tcctccaagt aaaacaattg attttctcct atgcttgtta
     9841 ctccacgcca aattgttgtt gttgctgttg ttggagtatt tcttgtctgt gtaatcaatt
     9901 caaccatttt acctttttga tttgttgtat agtaatcagc agtatagcta gatattggac
     9961 tattaccagt acctaccatt gtgccagaga tatcaacagg actagacttt actatctgag
    10021 gaaattcttt ttgtatttcc ttcatattct tcgtatattt ttcccatgaa ttccagttgt
    10081 aaaataaatt aaagaatttc ctactttgtt ccttgatttc agcatttccc aattctttgt
    10141 attgggcttc ttgcatagct ttcactttct ggttattctt gactacttcg atttgtttac
    10201 ttttaaggtc ttccaattct ttgacattat tatcataagc atttttcttt tgaaatagta
    10261 ccacacaaca aaggatcaaa attatggaaa gtattccgat cgccttttta gcagtagtaa
    10321 tttcatgatt ttgcataaaa agcaccccca gtcactttct tatcattatt tagtgtgatg
    10381 gttaatgtgg tatcatacac tttttttccg ttgtattgaa cttcgaaatc agtcaatgct
    10441 tcatacaacc cactactaga tgtcttaatt ccaaagtttg ttagcttaaa agtaaaaggt
    10501 ttatagtatt ctattttttc ttgctcgcta ctctcgttag taactggtgg cttacctgtc
    10561 acaataaata actttcgcaa aaattcttca cctgactgat tcgcaaattt gcttcggtct
    10621 gatcctttaa acatctcttg aatatcattt tctaaatctc tttgcaatcc atccacttca
    10681 tttggtttaa cctctttaaa gaaggtcaat tcattttcaa tttgcacact gtgcttttca
    10741 gcttctatgt cttgattctg cttattgaaa tatagaattc caacaagcat tgctaatagc
    10801 ataccagaaa gcaaagaaat gataattgca tctttcgttt tactattcat ctctgtcgcc
    10861 ctccattcaa atttttattt ttcaatttca aatttagctt tgcctacacc gctaaagaaa
    10921 tgcacattat ctgacccatc cacaccatca atcaaagtta ctgaaacacc tcttgttggt
    10981 gcttgggtag gtggaacatt gaaattagcg cacaaaaatg atccatcgtc aaacaccgct
    11041 ataactacac ctgtatgtcc tgctgatcca acagcccata accatccatc taaagcacta
    11101 aacgcataac ctacaattgg tttgttcgtc acctttgctc ctgccgattt gtagctttcc
    11161 caaacgtaac cgccatttcc caaagttggt tgatttccat catataattg actcatataa
    11221 gcccatgtta attcagtaca ttgtccttgt aattcacctt gaaaaggtga tgttgaccag
    11281 tctggttctt taaattttgg tagcgtgatt gctttcccat atttttttaa tgcttcttca
    11341 taagtcatat ttgctattac ttgacctttt ttaccgttaa tttttccttc tttcccaatt
    11401 gatccagaac cagaattagc atcatccgta gttgcacagg catcttctac tgttcctgat
    11461 gctgaacttg atgaattatt cgatccatct ccggtacttg aacctccgga tattttattg
    11521 atcttacttt catcttttgc accgtccata cccttgcttt tcatgtactt ccagatttct
    11581 tttgcatttt caccacgtac agctattaca gctgattcgg aatcagctga aatttcccag
    11641 tttttatgaa aagttactgc attttctact acatcatcag aagcatttaa agcataatct
    11701 tttaatattc ctacgaatcc cccatcacct ttgaacataa agtccaattg ggcttctgta
    11761 gtccaccaag aaccgccatt atttttctta gaccaatcaa ctaatgctgt atgtcgcccg
    11821 gctgtccatt gaccatagcc aatacccatt agaccatcag taaattgttt gatgaagtca
    11881 acttctctct gcccatcgcc gtaataccct tctactgttc gaggatcaat acctgattct
    11941 tgtttccagt tacctaaaat acctgcaata ctagcacctg ataagccata ttctccattc
    12001 atgtatttgt aaatatcttc aatatttttg gctacatcac ctgttacagc gccacttcca
    12061 gtatcgtttc catcgctatc tgttcctgac ccactacctt cagtagtaca gttaaattgt
    12121 gcatcataag tagctatccc atcagcatgt aacacttgcg gatttattgt gattacactc
    12181 attgcaatcc ctgtaaactg caccattaac gtaagcaacg tggcaatctt tctcttagat
    12241 tttttcaata actacgcccc caaaaagtgt acttttttat gttattatga taatataata
    12301 acataaatta atagaaagga aaagctaata aatgactgat aaaacaagtg atttacctaa
    12361 actagatcca aaaatatctc aagttaagag caaaaaagat ggaaaaacta aaaatgctgt
    12421 aaaggaaaca tcctcgttac tacttgatat tccaatgagt tcttttgatg aaatacttga
    12481 tgccaatacg gatgaaactt ctgttaaaat tgcatctcac gttggaatga acagtcaaaa
    12541 tgattatttt tacaacttgt taaacagcct aagaaagttg gaagataaaa aaaatcaggt
    12601 aatcgcttca aaagctcctg acctcacaac cgatttggat gctgaaatta tccaaatcaa
    12661 acaagatgag cgatcagtta aatcgctact gaaaacctac tttgatggtt ttgataaaga
    12721 actagctcct tatagccaag caatcctaac taaaccaata caagctccac atatttcaat
    12781 ttccctacaa aaaaagagtg atgactaaac agtcattact cttttttaga tttctggtaa
    12841 gcgctgatcg caaaaaccat caacaatatt gactcaaaaa atatgttcat tcttttctct
    12901 tttttccatt atagatttcc ctctattccg ttaaattgaa agtcacaccg attactacaa
    12961 agattaacat gaccacattc aaaaacatta aaagatatgc tgataatctc atcatttttt
    13021 tagttcactt actggtaatc taaacctttg agtgattgca ctggaaatac catattcacc
    13081 aattacaagc aaaaaatacg caataatttc agttccacct agatagatca gtaaaataag
    13141 taagtcactt aacattgtta gtttcctccc agtatatgat tttcattttt gggaagtctt
    13201 ttataatcca attcctcaaa aaaaggaacc atagtgtcac taatataggt tttagtttca
    13261 ggtactgcca aaattcccca atcaagaatt tcttcccatg atacatcatc aaattctaaa
    13321 atattatgat caactgacct attaccaagt tcatatccac caaaaattac catattatca
    13381 atcatgtata gaatttcatc cggcgaacca tgagttaatg tttcgtcctc ttcaagcagt
    13441 tcgtttaatc tttgtttgta ttcttctctt ttcatacttt tatcccttct tactcatttt
    13501 tttgaatttt aacattttta atccaagtct gcttggtatc tctgtagtag tcatgcgctt
    13561 ttattgtcgc cttaactttt cttatttctc caacatccca ttcatcataa taaagaaatt
    13621 tatttgtctt ccaaatcaat gaattatggt tttgatcttc aaataaatgc aggtaaacat
    13681 tgccccattg tgtatctatg gtgacggtat tggtaagttt caaatccaat tccaatcgtt
    13741 gagcgattgt accaataaac tcaccctctt ctctacgctt cgcacttaaa atctcctgtt
    13801 gtcttttgct gtattcttct agcttcaact caataattcc ttcatcattg attacagaaa
    13861 attcttctac aaaaactttt tgagttttgt attgttcttg tttttcctta aaaaaaccaa
    13921 cctaataaag gatcataagt agctccttga tgatgcagtt catctttgat cgtatagcta
    13981 tttttctcta taaccacgta aatataaccc ttttcaaagc cataacgatc aaaatttttc
    14041 tgagattctt tctctcttgc gatccttatt tttcgatcag ctcttttttt atttaaaaca
    14101 tttagcttag ttctatattc tggcgtaaag gatttttcag ttacaacaag aaatccatca
    14161 ccatcgcact taaaacatgt gccataggta attttcccca tagacataaa cttgtactga
    14221 cctgatccgc tacaacgata gcaagggatt tttttcttca tgattatagt tccattttta
    14281 tccggttgcg atttgctgat gatttcttct tgactgaaca tcctccatac tcctttctga
    14341 gcgaggataa aaccttgctc atcttcaggt tgtttaaaaa ctaggatcgt agtatttttc
    14401 tctatcacct atataaaaat gaatatcttc tttaagcact ttccatccat tttttgtctt
    14461 aactaactcc aatatttgac cttcaggatt tctgctatat tcccactctt gatctggaaa
    14521 agtagcttta ttgattgctc catccgcttg aaccaaaatt ctgtttttgc tttttacttc
    14581 aattactgta taaggatttc tgtcggtata tagaagctcg gttgctccca ttccaactac
    14641 aatctccttg tttgttgtac tgccttccat cattctattt tgtaagctac cttctaattt
    14701 catttaagat caccctttct caacttatct acaa
//
LOCUS       DAFGFZ010000069        14629 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000069 DAFGFZ010000000
VERSION     DAFGFZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.070"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            2..814
                     /locus_tag="LR726_001859"
     CDS             2..814
                     /locus_tag="LR726_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL6216945.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            811..1611
                     /locus_tag="LR726_001860"
     CDS             811..1611
                     /locus_tag="LR726_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL6216946.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1772..3040
                     /gene="rseP"
                     /locus_tag="LR726_001861"
     CDS             1772..3040
                     /gene="rseP"
                     /locus_tag="LR726_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL6216947.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3108..4817
                     /locus_tag="LR726_001862"
     CDS             3108..4817
                     /locus_tag="LR726_001862"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL6216948.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5029..9387
                     /locus_tag="LR726_001863"
     CDS             5029..9387
                     /locus_tag="LR726_001863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL6216949.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9532..10005
                     /gene="rimP"
                     /locus_tag="LR726_001864"
     CDS             9532..10005
                     /gene="rimP"
                     /locus_tag="LR726_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL6216950.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10028..11203
                     /gene="nusA"
                     /locus_tag="LR726_001865"
     CDS             10028..11203
                     /gene="nusA"
                     /locus_tag="LR726_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL6216951.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11225..11518
                     /locus_tag="LR726_001866"
     CDS             11225..11518
                     /locus_tag="LR726_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL6216952.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11515..11826
                     /locus_tag="LR726_001867"
     CDS             11515..11826
                     /locus_tag="LR726_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL6216953.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11839..14145
                     /gene="infB"
                     /locus_tag="LR726_001868"
     CDS             11839..14145
                     /gene="infB"
                     /locus_tag="LR726_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL6216954.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14172..14519
                     /gene="rbfA"
                     /locus_tag="LR726_001869"
     CDS             14172..14519
                     /gene="rbfA"
                     /locus_tag="LR726_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL6216955.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3254 g         3794 t
ORIGIN      
        1 aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt
       61 taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg
      121 ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa
      181 gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc
      241 gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga
      301 cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg
      361 ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac
      421 aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagta gagcagagat
      481 cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga
      541 aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca
      601 agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg
      661 gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc
      721 gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa
      781 aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtgatcac agcagctgtg
      841 gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca
      901 gcaatcttag cggtagtagg agtttacgag ctgttccgga tgaaaggatt gacactctta
      961 agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga
     1021 tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg
     1081 attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt
     1141 cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca
     1201 gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca
     1261 tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa
     1321 acgattgaag gtgcgctcgg tggtatcggt agtgctgttg tagtcgcatt tctttattta
     1381 ttgctatacc cagcaaaaga actattcaat catggtatgt ttgtcatgct tctgatgacg
     1441 attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat
     1501 tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc
     1561 gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac
     1621 ctgcgtttct ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac
     1681 tattggtgtc tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa
     1741 acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt
     1801 ttttggtata ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg
     1861 aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa
     1921 agacgggaca acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg
     1981 caatggggat gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc
     2041 tgacggcatc gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc
     2101 gatggaattg agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg
     2161 agacgaaaca gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga
     2221 cggaacagaa atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca
     2281 gcgtatgctg accaactttg ctggacccat gaataacttt attttggcaa tcgtgttatt
     2341 catcattctg gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga
     2401 gatcatgcct aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag
     2461 tgtggacgga aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc
     2521 aggtaaaaca ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac
     2581 accgaaatct gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat
     2641 gaatacaggt tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt
     2701 ggaaattttc aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg
     2761 acctgtcatg atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat
     2821 tagtttgatg gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc
     2881 cttagatggc gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag
     2941 tcaagaaaaa gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt
     3001 gctagttaca tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt
     3061 ataataacaa acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa
     3121 aaatgttgat tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa
     3181 tcttgctaag agctggatat atccgccaag tatcagcggg tatttattca tatctgccat
     3241 tagcaaatcg tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg
     3301 gagcggtaga aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc
     3361 gttacgaaac atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga
     3421 tccttggacc aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat
     3481 ataagaaatt acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac
     3541 ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa agatgcatat tctttccatg
     3601 caacggaaga aagtttggac gagacatata aagattacga aaaagcctat accgaagtat
     3661 tcaagcgttg tggcttagaa tttcgctcga tcatcggaga cggcggcgca atgggcggga
     3721 aagactcaaa ggaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga
     3781 ctgaaagtga ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat
     3841 cgcatgaaac acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg
     3901 aagtagcaga tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg
     3961 cggatgaaca acctgtcctt gttttggtac gtggagacca tgaagtcaat gacgtaaaac
     4021 tgaaaaactt tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat
     4081 atctaggtgc tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat
     4141 atgcagaccg ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat
     4201 tccactataa aaatgtgaat ccagaacgcg atttcactgt gctttcttat gaagatcttc
     4261 gttttgtaca agaaggagat ccttcaccag acaacaacgg tgttcttgcc tttactcgag
     4321 gaatcgaaat cggacatata ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa
     4381 ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg
     4441 taagtcgttt attatcagca atcgtagaac aacattcaga tgaacaagga atcaattggc
     4501 caaaaggaat tgcgccattt gatctgcata tcgtccaaat gaatctaaaa gacgaatacc
     4561 aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg
     4621 ttgatgacag aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc
     4681 caatcagaat cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga
     4741 aaactggcga aatggttgaa gtacgtaaag aagaattggc gaatacttta ccaatcctct
     4801 tgaaccaagg cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt
     4861 ttttgacaat ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa
     4921 ttttttaatg taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga
     4981 taagatgcga tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa
     5041 cgagatttat ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat
     5101 ccactgctta ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg
     5161 gaatttacgc tacatttttc caaaattttg cctattatgc tatatcggtc attgactcag
     5221 catctgacta tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac
     5281 cagacattcg atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa
     5341 tgcgatacac cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt
     5401 aaagtcatct tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg
     5461 ccattagtag aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa
     5521 gtggatgagc agcaagcgga acgagtattg aaactttttg aagaacgcaa acaagaacag
     5581 gcagaagcct ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag
     5641 gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc acatccagct aggacgaaat
     5701 attccagccg atgaaccaac gacaccaatg atcagtatcg ttgaagaaga acgaagagtc
     5761 acacttgagg ggtacgtatt cgacaaagaa gtcagagaac tacgttcaaa acgcaagatc
     5821 ttgactttga aaatcacaga ttatacttca tcatttatcg tcaaaaaatt ctcgaacggc
     5881 gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta gctggttaaa ggtacgagga
     5941 agtattcaag aagatacgtt tgtccgtgat ttggtcatga atgcgcaaga tatcatagaa
     6001 gtcaaacata cacctcgcaa agattatgct cctgaagggg aaaaacgagt agagcttcat
     6061 gttcatagca atatgagtac gatggatgca acaaacagca tctctgatct tgtggcacaa
     6121 gcagggaaat ggggacaccg agctatcgcc attacagacc acggtggtgc gcaagccttt
     6181 ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa tcttatacgg cgtggaagcg
     6241 aatgtcgtag atgatggtgt cccaattgct tataatgatg cacatgaagc attgagtgaa
     6301 gccacatatg tcgtatttga cgtggaaacg acaggattgt ctgcagtata tgacacgatc
     6361 atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga tcgaaagctt tgatgaattt
     6421 atcgatccag gacatccttt gtcacgaaca acggtagatc tgactggtat cacggatgga
     6481 atggttcgag gatcaaagtc ggaagaagaa gtattgcgga tgttccttga attttcaaaa
     6541 gatacaattt tggtagccca caacgcggct tttgatatgg ggttcttgaa tactagctac
     6601 gcaaggtatg gtattcctga agcggccaat ccagtcattg atacgttgga attagctaga
     6661 tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt tatctaaaaa atttggtgtc
     6721 agcttggaac agcatcaccg agcaatctat gatgctgaag caactggtca cttagcatgg
     6781 atctttgtga aagaggcaat ggacaatcat aacatgcttt atcatgatca gctaaatgag
     6841 catatcggag aaggagattc ttacaaaagg gctcgacctt tccatgtaac gattttagca
     6901 aaaaatcagg caggactgaa agatttgttc aaactgattt caatgtcgaa tgtcgaatac
     6961 ttcgaacgcg tcccacgaat accacgctcc caattgaaaa agatgcgtga aaatctattg
     7021 atcggctcag cttgtgataa gggagaaata ttcgaagcca tgatgcaaaa aggggtagaa
     7081 gaggcaagaa accgagcaaa attctacgat tatattgaag ttatgccaaa agcggtctat
     7141 gcgcctttga tcgagcaaga attggtaaaa aatgaacatg atttggaaga aattatccaa
     7201 aatttggtag aaatcggaaa aagtttggat aaaatcgttg ttgcaacagg taatgtacat
     7261 tatctgaacg aagaagatgc catttaccga aaaattttga tcaattctat gggcggagcg
     7321 aatccgttga atcgtcatag tttgccggat gtccatttcc gaacaaccga tgagatgcta
     7381 actgcatttc actttttagg ggaagaaaca gcgaaagaga tcgtagtaga aaatacgaat
     7441 aaaatcgcag atatctgcga agaagtgatt ccagtcaaag atgaacttta tacaccgaag
     7501 attccaggtt cagaagatga aatatcagaa ttgagttaca caaaagcaaa acaaatgtac
     7561 ggagatccgt tacctgagat cattcaaaaa cgattaaaaa aagagttgaa ttctattaat
     7621 ggtaatggct tctcggttat ttatctgatc gcccaaaaac tagtgcataa aagtaatgaa
     7681 gatggctatc tagtaggttc acgtggctct gttggctcta gctttgttgc gacgatgaca
     7741 ggaatcactg aagttaatcc gttagcccct cattattatt gtccagagtg ccaatattcc
     7801 gagttttttg aagatggtac gtatggttct ggatttgaca tgccagaaaa acaatgtcca
     7861 aaatgtggtg cacgcttaaa caaagacggt catgatattc catttgagac atttttagga
     7921 ttccatggag acaaagtgcc cgatattgat ttgaacttct caggtgatta tcaagcagaa
     7981 gcccataact atacgaaagt attgtttggc gaagactatg tttaccgtgc cggaacgatt
     8041 ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag gatacgaaag ggacaataat
     8101 ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag gcgctactgg agtcaaacgg
     8161 acaaccggcc agcatccagg ggggatcatc gttattcccg attatatgga tgtctatgat
     8221 tttacgccga tccaatatcc agcagacgat caaaattcgg aatggaaaac gactcatttt
     8281 gattttcact cgattcacga taatgtattg aaattagata ttctagggca tgatgaccca
     8341 actgttattc ggatgcttca agatttatca gggatcgatc cacaaacgat ccctacggat
     8401 gacccggaag tgatgcggat atttgccggt cctgaagtac ttggagtaag tcaggaacaa
     8461 atttattcca aaacagggac attaggtatt ccagaattcg gcactcgttt tgttcgaggg
     8521 atgctagaag aaacgcatcc tacaactttt gcagaactac tgcaaatttc cggactatct
     8581 catggtacag acgtatggtt aggaaatgcg gaagaattga ttcgacgagg agatgcgaca
     8641 ctggcggaag taatcggctg tcgtgatgat attatggttt atttgatcca tgctggatta
     8701 gatagcggga tggcatttaa aatcatggaa actgttcgta aaggacaatg gaacaagatc
     8761 cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag aaaataatgt gcccgactgg
     8821 tatatcgatt cttgttcaaa aatcaaatat atgtttccga aagcccatgc tgcagcttac
     8881 gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt tccctatttt gtattactgt
     8941 gcatactttt ctgtacgtgc agacgacttt gatctagtct ccatgtgcaa aggaaaagat
     9001 gctgtgaaac aagcaatgaa agaaatcacg gacaaaggat tagatgcttc cgtaaaagaa
     9061 aagaatcaac tgacagtgct ggagttagcc aatgaaatgc tggaacgagg attcaaattt
     9121 ggaatgattg atttgtataa atcggatgcc gttaactttg ttattgaagg tgatacgttg
     9181 atcgcaccat ttagagctgt cccaagtttg ggaaccaacg tggcaaaaca aatcgttgaa
     9241 gcaagaaaag acggtccatt tttatccaaa gaagatcttg ccacccgcgg taaagtttca
     9301 aaaacattga tcgaatatat gaacgacaat ggcgtattga aagatctacc agatgaaaat
     9361 caattatcgt tattcgatat gctataagca tctgttttcc tgtctgcttg taattttaag
     9421 cagagtatgg tatagttctt ataggtaatg atactaactg tgagtgagcg gatttttcgc
     9481 tcactctttt taatggaatt acgcacttaa ttttttaagg aggcgaaact tttgagtagc
     9541 gtcgttgaaa cagtcacaga aatggtgaca ccgattttag atgaacaaaa ttttgaactt
     9601 gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct tacgagtatt tattgacaaa
     9661 gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg agaaactgag tgaaaaactt
     9721 gatgcagcag atccagatcc gattcctcaa gcttatttcc tggaggtatc ctccccggga
     9781 gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg cacttggtga ttatgtccat
     9841 gtctccttat accaaccagt agatggcgaa aaacaatatg aaggatttct tcaatcattt
     9901 gatgctgaac agcttacatt gaaaatacgc ataaaaacac gagaaaaaga attgactttt
     9961 gaccgaaaaa atattgcaaa agctcgctta gctatccaat tttaatcacg gagaggaatc
    10021 agaaaaaatg agtaaagaaa tgttaaatgc gttagacgct ttagaagctg aaaaagggat
    10081 ctcaaaagaa atcgtgatcg atgctttaga agcagcattg gtttctgcat acaaacgtca
    10141 ctatggccaa gcacaaaacg tcgaagtgga atttgatcag aaaaaaggaa agatccatgt
    10201 ctatgcggta aaagaagtaa cggaagaagt gatggattca cagttggaag tctcattgaa
    10261 agatgcactt ttaatcaatc cagcatatga gatcggtgat acgatccgtt ttgaagtaac
    10321 accaaaagat tttgggcgga tcgctgcgca aacggctaaa caagttattt tacagcgtgt
    10381 acgtgaagca gaaagaacga ttatttataa cgaattcagt gcttatgaaa aagacatcat
    10441 gcaaggaatc gtcgaacgcc aggataaacg ttatatttat gtcaatcttg gtaaaattga
    10501 ggcagttctt tctaaacaag atcaaatgcc aaatgaattt tatcaaccac atgatcgaat
    10561 caaagtttat gtttcccgag tagaaaatac gtctaaaggt ccgcaagtat ttgttagccg
    10621 cagccatcca gacctattgc gccgcttatt tgagcaagaa gtaccagaag tctatgatgg
    10681 aatcgttgaa attgtcagca tcgcccgtga agctggcgat cgttcaaaag tggcagttcg
    10741 ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc gggccaaagg gacaacgagt
    10801 tcaagcaatc gtcaatgaat taaaaggcga aaacatggat atcgtcgaat ggaatgaaga
    10861 tccagctgta ttcattgcaa atgcattgaa tcctgcacaa gtcatggatg tgatcttcga
    10921 tgagacaaat ccaaaggcct gcacagttgt cgttccagac taccaattat cgctagcaat
    10981 cggtaaacgc ggacaaaacg cacgtttagc tgctaaattg actaaccata aaatcgatat
    11041 caaatcagaa tctgacatga cggaatttta tgaaaatcaa gaacaacaaa aagaaacaga
    11101 agaactgcat gatgaagcaa tcgttcagtc agatttaaca gacgatgaat atgaaacaat
    11161 tgcattcaac aatgaaacgg tcgaagaaaa accagaagct taactttagt aaggaggcaa
    11221 agagatgaaa caaaggaaaa ttcctttacg caaatccgtg gtttcaggtg aaatgaaacc
    11281 taaaaaagag atggtccgaa tcacccgttc aaaagaaggg gaagtatcga tcgatccaac
    11341 tggaaaaatg cctggacgag gagcgtatgt ctcacttgag ccggaagaag tacaacaagc
    11401 ttgggataaa aaaattctcg atcgagtact cgaaacaaca ttaacagatg agttttatca
    11461 agaacttctt gactatgtca ctcaccaaaa agcacgtaaa gagttatttg gaaaatgaat
    11521 acagttaatc ggcaaaaagc catgaatctc atcggtctag cgatgcgcgc gggaaaacta
    11581 atcacgggtg aagaactgac cattgctgaa atccgcaaac aaaaagcaaa attggtgttt
    11641 gtggcaaccg atgcaagcga gaatacaaga aaaaagataa aagataagag ttcatattat
    11701 gaagttcctt gctttgagct attttctgaa gcagaaatca cgcagatgat cggaaaacct
    11761 cgcaaggtat tcggaataat ggatgccgga tttgcgaaga agaccaagga gctaattgaa
    11821 ggttaggaag gtgattgcat gggcaacaaa agaatctatg aattagcaaa agaactcaaa
    11881 aaatctagta aagacgtcgt ggaaaaagca caacaacttg gcatcgatgt gaaaaaccat
    11941 atgggtgcga ttactagcaa tgatgagaag aaattgcaac aagcgttcaa aaacccaagc
    12001 acgaaacaac aagctgcaca acaagcaagc agaaaaccag ccaatcaaca gccgaacgat
    12061 caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata aaaataaccg cagttatcaa
    12121 gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa aaccaacaac aaaccaaaat
    12181 aatacgggaa ataccagcag ccaaaatcgt caaggaacga gccaaggcgg caatcaaaac
    12241 cgtactaaca ataacaacaa accaaacaat aacaatcgcg gcaaatttaa taataataac
    12301 cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc aacaatcaac taaaccagct
    12361 gtaccaccac gcaaattccg tgaattgcca gatgtattag agtatactga aggtatgaac
    12421 gtagcagata ttgcgaaaaa aattcatcgt gagccagcag aaatcattaa aaaattattt
    12481 atgcttggtg tcatggtcaa tcaaaaccaa gcactggata aagatacgat tgaactgtta
    12541 gcgacggact atggcatgga accacaagaa aaagtccaag ttgacattgc tgatatcgat
    12601 aaattcttcg aggcagaaga agtcgatcca gataaattag tctcacgtcc gccagtcgta
    12661 acgattatgg gacacgtaga ccatgggaaa acaactttat tggatacatt acgccattca
    12721 cgtgtaacaa gcggcgaagc cggcggaatc acacagcata tcggtgccta ccagatcgat
    12781 attgatggta aaccaatcac tttcttggat acaccaggac atgcggcctt tacaagtatg
    12841 cgtgcacgtg gagcaagtat cacagatatc acgatcttag ttgttgctgc agatgatggc
    12901 gtcatgccac aaacagttga agcaatcaac catgccaaag ctgcaggcgt tccaatcatc
    12961 gtcgctgtaa ataaaattga taaaccaggt gcaaaccctc aacatgttat gcaagaatta
    13021 agtgagtatg agttaattcc tgaagcatgg ggtggagaaa cgatctttgt tgagatctca
    13081 gcgaaattcg gccaaaacat cgaagaattg ctggaaatga tcctgttagt cgcagaagta
    13141 gaagatttaa aagcagatcc aacacaacgc gcaatcggaa cagtgatcga agcacgtctt
    13201 gataaaggta aaggtcctgt gtcaacctta cttgtccaag aaggaacact tcatgttggt
    13261 gacccaatcg ttgtcggtaa tacttacgga cgtgtccgtg taatgacaaa cgatcttggc
    13321 cgtcgcgata aagaagcagg acctgcaacg ccagtagaaa tcactggctt gaatgatgta
    13381 cctcaagcag gtgaccgttt cgtagtcttc gaagacgaaa agacagcacg tgcagcgggt
    13441 gaagaacgcg cgaaacgtgc attattagaa aaccgtgctg ccacaagccg tgtgactttg
    13501 gataacttat ttgaaagcct gaaagaaggc gaattgaaag aagttaacgt aatcatcaaa
    13561 gcagatgtac aaggttcagc agaagcactg gctgcatcat tgaagaagat tgatgtggaa
    13621 ggtgtccgtg taaaaatcgt ccattcagct gtcggagcaa tcaatgaaag tgatgtaacc
    13681 ttagcagcag ccagcaacgc tattatcatc ggtttcaacg ttcgtcctac accgcaggca
    13741 aaagtgcaag ctgatactga agaagtcgat attcgtcttc accgtatcat ctataaagcc
    13801 atcgaagaaa tcgaaactgc gatgaaaggg atgctagacc ctgaatttga agaaaaaatc
    13861 accggtcaaa tgatcgttcg tgaaaccttc aaagtttcta aagttggtac gattgcaggt
    13921 gcctatgtaa cagaaggata tattcgtaga gatagcggtg tccgtgtaat ccgtgacgga
    13981 atcgtaatct atgaaggcca acttgcaagt ctgaaacgat tcaaagatga cgtgaaagaa
    14041 gtcaaaatgg gttatgaatg tggtgcaatg attgaaaaat tcaatgacat caaagtagat
    14101 gacgtaattg aaggatttat catggaagaa gtcaaaaatg actaaaaact gataaaagga
    14161 ggccaatcat tatggctaac taccgtgacc gccgagtcgg tcaagaaatc ttaaaagaag
    14221 tcaatgatat tttgcgcaaa aaggtccgtg accctcgtgt ggagaacgta acgatcacag
    14281 atgtgaaagt tacgggggac ttacagcaag caacgatcta ttacagcatt ttatctgact
    14341 tagcatcaga taagaaaaca gcacaagatg gattgaataa agcatcagga ttgattcgtc
    14401 gagagttagg gcacaatcta agtatttata agacacctga attgttcttt gaacttgatg
    14461 aatcagttgt ctatggcaac catatcgatg aattgcttcg cgacttgaac aaagactaat
    14521 caaaaactcc cgcaaatcct gtgaaatcag gctctttgtc aataaggact gatgagttgt
    14581 gcaaaatcaa atctgagtca gaatgaacca cattctggct cagattttt
//
LOCUS       DAFGFZ010000070        14625 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000070 DAFGFZ010000000
VERSION     DAFGFZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.071"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(186..2261)
                     /locus_tag="LR726_001870"
     CDS             complement(186..2261)
                     /locus_tag="LR726_001870"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL6216956.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2263..3180)
                     /gene="glyQ"
                     /locus_tag="LR726_001871"
     CDS             complement(2263..3180)
                     /gene="glyQ"
                     /locus_tag="LR726_001871"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL6216957.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3566..4384)
                     /gene="recO"
                     /locus_tag="LR726_001872"
     CDS             complement(3566..4384)
                     /gene="recO"
                     /locus_tag="LR726_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL6216958.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4527..5426)
                     /gene="era"
                     /locus_tag="LR726_001873"
     CDS             complement(4527..5426)
                     /gene="era"
                     /locus_tag="LR726_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL6216959.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5441..5836)
                     /locus_tag="LR726_001874"
     CDS             complement(5441..5836)
                     /locus_tag="LR726_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL6216960.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5820..6296)
                     /gene="ybeY"
                     /locus_tag="LR726_001875"
     CDS             complement(5820..6296)
                     /gene="ybeY"
                     /locus_tag="LR726_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL6216961.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6325..8517)
                     /locus_tag="LR726_001876"
     CDS             complement(6325..8517)
                     /locus_tag="LR726_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL6216962.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8541..9512)
                     /locus_tag="LR726_001877"
     CDS             complement(8541..9512)
                     /locus_tag="LR726_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL6216963.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10217..11596
                     /gene="brnQ"
                     /locus_tag="LR726_001878"
     CDS             10217..11596
                     /gene="brnQ"
                     /locus_tag="LR726_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL6216964.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11878..13218
                     /locus_tag="LR726_001879"
     CDS             11878..13218
                     /locus_tag="LR726_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL6216965.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            13375..>14625
                     /locus_tag="LR726_001880"
     CDS             13375..>14625
                     /locus_tag="LR726_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL6216966.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         4098 a         3104 c         2699 g         4724 t
ORIGIN      
        1 cagtaacaag attataatta atcgttttca tttttatttc ctcacttttc atgagcgttt
       61 tgaaaattcg aacaaagaat aaaataacaa aaattattaa aatagaatta ataacagcta
      121 tttataacaa aaaaaacaga gacacagcac gtttcgtgca tgctctctgt ttttcaaaca
      181 aaagactatt ttgtgatgat ttgatccaag cttgctaatg acaaagccat ggcagctatt
      241 tgacttagtt gtgttagtcg gttcttacgt actgctggtt catcagccat gaccatcgtt
      301 tgatcaaaat aattttcgat cagtggacga agagcgacaa gctggtcgta gttttcgtgg
      361 atcgtttggt cagcaaaact tgctttcatt gcttctactg ctttgtgtaa ttctttctct
      421 gcgtcgtttt cgaacagctc agggtctact tctgattgtt tttcaagctg cgcttttttc
      481 gctaagttca cgacacgtgt caaagcttcc attgaaggtt tgaagtcttc atctgcaaga
      541 tgttgtttga acaattgtgc tgctgcaaat acttttgtca gatctttttg ttccgcattc
      601 aaaacagcat cgatgacatc atgacgaata ttcttcgttg tcaataattg gcgcagacgg
      661 cctttgataa agtcgatcac ttgttgttgc ccttctgata aaccaatgcc gaaacgatcc
      721 gtatctttac tgataacatc gctaatcgtt tcttgcagaa cagacaaagg aaaggcccat
      781 tttttcgatt cgatgatccg aacgacaccg tatgcttggc gacgtaaggc ataaggatcg
      841 tttgacccgc ttggaatcat cccaactgca aagaaagaaa agatgctatc taatttatct
      901 gcaatcgcta aaacagaacc tactgtagac tgaggtagct ctccttcact ggatataggc
      961 atgtaatgct cacgaatcgc tgtcgctaca gcaggtttct cgcctttcag aagagcatat
     1021 ttttcgccca tgatcccttg tagttcagga aattccccta ccatgttggt tactagatca
     1081 aatttataga tttcagcagc tcgttgcaaa tccatcaatt cttcctcaga tagtcctact
     1141 tgttcgccaa tgatacgggc aatgacttgt acccgttgca ttttctcata gatactaccg
     1201 attttttcat ggaaagtaac agtttttagt ttttcaacgt attggtcgat tgaagctttc
     1261 ttgtcttcat tataaaagaa ttccgcatct tctaaacgag ccgtcaatac tttttgattg
     1321 cctcgaacca catcttccaa ttttacccga tcaccattac gagcagaaat aaaatgagga
     1381 agcagcatac cttgatcatt ccgtacctcg aaataacgtt gatgttcttt catagaagtg
     1441 actagtactt cttcaggaac agaaagatat ttttcttcga agtcgccaga aaagacagtt
     1501 ggatattcga ctaaattgtt gacttcttct agtaaatctt cgtctagatc aataacccag
     1561 tgattttttt ctgctaattc gtttgcttgt gtaacgatca tttcttgtct tttttgcgga
     1621 gacgcaatga cgaattgttc attcaatttt tcttcatatt cttttgcatg cgcaaatgtc
     1681 acatcttcgc ctaagaaacg atgacctcga gaggctctgc ctgttttgac atccaagatt
     1741 tcgaatggga ttacttcgtc atctaacaat gccacgatcc agtgaatagg gcggatatat
     1801 tcaaaatcgt attctgccca atgcatcgtt acagggaaag tcaaactttt gatcacccct
     1861 gctaagcctg tcaatacttc ttttgcttcc ttaccatgag tgtatttcgt tacatagaca
     1921 tattccacac cttttaattc tctaaaagtg atagcgtctg tcgtcagacc ttgtccgcgg
     1981 acaaatcctt ccgctgcttt cgtccagttt ccatctgcgt ccaaagcaat ttttttggct
     2041 ggtcctttga cttcttcttc actgtccgct tgtcgttctg gaatatttgt tactttgatt
     2101 gctaaacggc gcggtgtgga aaatgtttcg atcgtatcat aagtcaattg gttttcatct
     2161 aaaaattttg ctgttttttc ttctaattgt ttcatgcttg gtgtgacaac atgggcaggc
     2221 atttcttcca gcccgatttc aagtaataag tcttttgcca tcttattcag cctccttcac
     2281 ggtttctgga tttgctgtgt ttttattcaa caaagggaat cctaattttt cgcgttctgc
     2341 cacaaagatt ttggctacag cacgagccat gttgcggatg cgagaaagat aaccggcacg
     2401 ctctgttacg gatactgcac cgcgggcgtc taataagttg aatgtatggc tacatttcaa
     2461 gatataatca tacgccggat gaacaagatt ttcatcgata cagcgtttag cttctttttc
     2521 aaaacgatca aagttgctta gtagcaagtc ttggtcgctt acttcaaaag aatatttgga
     2581 atgttcatat tctggttgtt tgaagatctc gccatatttc acgccatcag cccattctaa
     2641 atcatagaca ctttcaactt cttggatata agaagcaaga cgttctaatc cataagtgat
     2701 ctcagaagtt actggtttac aaggcagtcc accaacttgc tggaagtacg tgaattgtgt
     2761 gatttccatt ccgtcaagcc atacttccca acccacaccg gcacagccca tggatgggtt
     2821 ctcccagttg tcttcaacaa aacggatgtc gtgttctaat ggatcgattc ctaaaaggcg
     2881 caagctttct aaataaagct cttgaatatt ctcaggtgaa ggtttcatca ccacttggaa
     2941 ttgatgatgc tgatacaaac ggtttgggtt ttcaccataa cgtccatcag ctggtcggcg
     3001 agatggctct acgtaagcag cattccatgg ttctggtccg attgcccgta aaaaggtata
     3061 tgggctcatc gtacctgctc ctttttctgt gtcataagct tgcatcagca tgcagccgtt
     3121 tgatgaccaa aatttttgta atgttaaaat catatcttgg acggtcaatt ttttactcat
     3181 ctgttttgcc cctttcaatt ttaaagcgtt gctgtcaaat aagaatacag gaaagaaaac
     3241 gacaaaaaat ccctatgtct gattacagac atagggacga ataattcgcg gttccaccct
     3301 acttccgaat aaagattcgg cagcttcatt tatcgtactc gcaagtgcct cttcaaaaat
     3361 cgtttgggtc cggctcccac tctcccgaac tcgctaaacc acgtgttttt tactgatctt
     3421 ggtcaatgta cgtattcatt ttgcagtttt cttgtttcta taatagatcc tatcactgaa
     3481 cgatctattt aatctatggt taataataga cggaactccc tgtgtttgtc aaatggtttt
     3541 gattttttgt tgtatgtttt atttactatt cttctttttc aggtgtctct ttttcttttt
     3601 cagacatgtc tttctctgac gtctttaata cgtgtgccca agacttcatt tgattgatga
     3661 actttttgct tttcaaatgg ataccgacat attcatcata cagctggtcg atgagttcac
     3721 ggattgcttg tttggtttct tctttcagat ggattccact aattttatca taagaaatag
     3781 ctgaaaacat ccgaacgaaa taaacagctc gaggatctgc atgataacgg tgatcgtcca
     3841 tcgaccaatg tctttcgcat atcacaccac catatttgga agaatagtca aattttcctt
     3901 ttgtttcccc gcatacgacg cagtgtgtcc agattggtgc aattccgaag cgttgcagta
     3961 tttggacttc aaaaatattc gtgatgattt ctgcatcgtc tccttggtcc atccgttgca
     4021 gtgcttgttg cgtaaactgg aacaaatgcg ggtcgtactg atgatcttcg attgccgcat
     4081 ctactagatt caataaatat gtgccatagc cgctcaaaaa gatgtcttct tggatgtgtc
     4141 gaaacgcatg gacatctttg acactgctta aaaatgacaa accttcactt cgaaaattcc
     4201 cgatataaac tgcttcagta aaagggagca aagccggagc taagggctga ttttgccgat
     4261 ggatctcttt gatataaaac atcattttgc cagaagattc tgtaaagatt ttgactaatt
     4321 tatctttttc tttgtaatta cggctagata aaatcaagcc ttttgattct gcaagctggc
     4381 tcatattctc cctcctaaaa aataaatctg gaaaagcaga ttctgccgat ccagatcatt
     4441 tatgttgcga tcttccgcag cttttctcaa cacgcggatt tttattcagt ttatttctat
     4501 acctaattta caagaagctc agaatatcag tattcgtctt ttcggtagcc gaagtcttgc
     4561 agatagactt ttttgtctcg ccagtctttt tgaactttga cccaaagttc tagaaagact
     4621 ttgtctccta aaagattttc gatatctttt cttgctttcg ttcctacttg cttcagcatt
     4681 tttccgcctt tgccgataat gatccctttt tggctatctc tttcgacaat aatcgtagcc
     4741 tggatatgga ttttatcatt ctcgtttcgc ttcatcgaat caacaacgac tgcgacagaa
     4801 tgaggcactt cgtctcgtgt taaaaacaag actttctcac ggatcaattc agaaacgatg
     4861 aagtattcag gatgatcagt cacttgatca tctggaaaat attgtgggcc ctctggcatt
     4921 tcactgacta atacatccat caaacgttcg acgttgtttc cttctgtagc agagattgga
     4981 atcacttgtg caaaaggcat ctgcgttgaa taatcttcga tgattcccaa caactcgtct
     5041 ggatggattt tgtcgatttt attgataatc agataaacag gagaatgcac gttttttaga
     5101 cgttcaataa taaaatcgtc tccccgaccg cgtttttgat cagcactaat cataaataag
     5161 accgcgtcca cttctttcaa cgcactgtaa gctgcttcta ccataaaatc tcctaaacga
     5221 tgctttggtt tatggattcc tggtgtatca ataaaaatca actgagcttc tggtgtagtg
     5281 tatactcctt ggattttatt tctagttgtc tgtgccttgt cactcatgat ggcaattttt
     5341 tgacccacga ttcgattcaa taatgtggat ttccctacat ttgggcggcc gacgattgcg
     5401 acaaaccctg atttatgttc tggcatatat gttctccttt ttaattaaac aacaaatgat
     5461 atattttagg gacaaataaa atcaacccaa cgatgacggt aaaacaagcg gtcactaaaa
     5521 ctgctccagc tgccatatct ttgatttttt tcccaatcgg atgaaaatga aaatccgtaa
     5581 acatgtccac gacattttca aaaacagtat tgatgatctc tactatccat acgagaaaaa
     5641 cagctaaaag cagccataac cattctatcc gatcaagttg gaaaatcacg cccataaaca
     5701 aagcgaggat tccgaacgca acatgtttcc tcatgttgcg ttcttcctca aaaacagttt
     5761 tgactccttg gacggcaaat tccacggaca tcaggaaatg tttgtttttc tctacgtttt
     5821 tatcttttaa gtccataggc atctaagatc tctttctgta aaccaaacat ttctttctca
     5881 tcctctggtg tcatatggtc atacccgttg atatgcaaga atccatgaac cgctaaaaag
     5941 ccaagttccc gatcatacga atggccgtaa gcttctgctt gctctgcagc acgttcagta
     6001 gagatcatga tgtctcccag tacacgaggc aatggattct ctccatcttc aaaaatcact
     6061 gggatttcgt cttcgccctc ttcttccaag gcaaaactga tgacgtctgt cggtgcatct
     6121 tttccacggt aatcacgatt gattacttgg attgctgcat tgtccatgaa agtaacagac
     6181 atttctgttt cttctggcaa ctctaaatat gatgctgcaa attgcagcaa gtcgtcgatt
     6241 tctttcattt tctcttctga gacttggttt gtttcatcca tgaaggtaat atccattata
     6301 catcgcttct ttctattctt cttgctattc ttcttgttct tcttttatct tctcaacttc
     6361 cgatttcatt ttaggatatt tgatccgaga atggaaggta ctgcttaaac cgctgatcaa
     6421 tgatttttct actttacgga tttcttttaa cgtcaatcca ctgtcgtcca attgtccatc
     6481 ggaaatacgc tcttcaataa gattatggac aaaactttcg attttttcgc tcgttggatg
     6541 gtccattgcc cgtactgccg cttcacaact gtcagctata ttgacgattc ctgcttcacg
     6601 gctttgaggt tttggtccag gataacggaa ttgctcttcc gtaacttccg ggttgcgttc
     6661 ctttgcctta acgtagaaat aacgcatcag cgtggttcca tgatgctgtc tacaaatatc
     6721 gatcaccatt tgcggcatat tataatcttc caatattttg gcaccatctg tcacatgtcc
     6781 aaagataata tgtttactgt cttctggcaa taagaaattg tgcggatttt ccgctcctga
     6841 aggcaagttc tcaacgaaaa agctggcgtg tttgatcttg ccgatatcat gataatagca
     6901 agctactcga gttagtaaag aacgtccacc gatttctgct acagcattag cacttagatt
     6961 ggctaccatc atgctatggt gatacgtacc tggtgcttct tccagcaact gtttcaataa
     7021 aggatgattg ggattgctga gttcatttaa tacgatcaca ctatcatcat tgaccatcaa
     7081 ttcaatatag gggtgcagac ctatcgtcag caagaatgaa aacagtgcac ctgtgaatcc
     7141 gcagactagc gttaaccaag tcgttgcatc cccaaagctc attccttggt agataatcaa
     7201 aatgacatcc atcatcacag gaaaaataat gacccacata gcagcggata acccttgttc
     7261 tgagatccgt ttgcgtctga cgatcgttgc catgagacca gaaaatacat aggacatcaa
     7321 aataactgtc agaaagttcg tgccaatcga atcataaaaa atgaaaagtg cggatactgc
     7381 ttgaaaaata ccagccatga tccctgctct tcgattcaag aaaaagctta aaacaagtgg
     7441 agcaaaagcc gcggggtaaa acagcggaat ataagcagct tgttctgttt ggaagagttg
     7501 gaaaaatttc attagtacga cgcttactga cattgccgtc acgtagaaaa gtacgtattt
     7561 tgttctttct cctgcatttt ggaattgaag tgaattataa atcagtacac cgatttgaag
     7621 caaaacagct aaaaccatgg caatgagtgg gaaaatagaa gtcgtttgat tcgtcaatcc
     7681 tagtaattcc aattttttca tcgctgaagc atcaatttga tttccttcgc gaacaataat
     7741 ttctccttgg aagatcatca ctggttgtac agcttctcgt gcagattgtt ttaattcttc
     7801 tgtctttttc tcattcaata catcattaac tacgatgcct tgatccacta agtagcgaat
     7861 catttgttgc tgctctgttg tgacattcaa atattgaatc tgatcttctg cttcttgttt
     7921 aaaagcttcc aattcgcttt cacgaacttg tttactcatc tgttcgtcaa tgagcttcaa
     7981 actttcatcc ctaactgtgt gaatttccgt ttcagtcatt tcaaatatcg tcgaataaaa
     8041 attgttcggc agtttttggt aaaacgcaac gttttctgca ttcacgtttt caaaattttt
     8101 cttcagtgta gcaatccgct cttctgtagt aggtttcggg atattttcat cttccttcgc
     8161 cttatctacc ttgccattat aagatttgtt gatggtatca ttcgtcttat cgatcaattc
     8221 gaataattgc ttgatccgat tgttctggtc atctgccaga tctttttgat aagtatattc
     8281 tggagtcact gcttctgctg ccagttttct tctttgttcg gtttcttccg tgttctcaat
     8341 cgttttattc gcacggatac tctcttctgc tacttggcct tctctgaaat caatggcctt
     8401 ttgtttgaca ctactaaatg taacgccgca gacaatgata aaaaacaaga acagaaggaa
     8461 aggcaatgct gcctttccga gtttttcttg tagtttctgc agtgttttat tcaacatgtg
     8521 cttatcctcc taagcaaagc ttattcttga tgaagatccg ccttttcata agctttgatg
     8581 atttcggcaa ctacagggtg ccgaacaacg tcacttgctt caaaattaac aaaatcgatt
     8641 tttttgattt gctttaatgt tcgttctgca tgaacaagac cacttgtcgt cccacgaggc
     8701 aagtctatct gacttgtatc cccattgaca atcatttttg attgataacc gagacgggtc
     8761 aagaacattt tcatctgagc gactgtcgtg ttttgtgctt cgtccaagat aacgaatgca
     8821 tcatccaatg tccgcccccg catgtaagct aaaggtgcga tttcgatcac gcctcgttcc
     8881 atcaatcggt tcgtatgatc caatccaaaa atttggtata aagcatcata aacaggccgc
     8941 agataaggat ccactttttc tttcagatcc ccgggtagaa agcctaggtt ttcccctgcc
     9001 tccacagcgg gccgcgtcaa aatgattttt tgcacttcgc ctttttttag ggcagcaatt
     9061 gctaatacta cggcaagaaa cgttttccct gttccggctg gccctacacc aaatacgatg
     9121 tcatgtttgg ctaccgattc aacgtatttc ttttgaccgc tgttttttac ccgtataggt
     9181 tttccattac ggtctttgac gatctcttcc tcatacatct caacaaaata atctaatgta
     9241 cctttttgcc ccattttcaa tgccgtcaca acatctggcg tacttacgtg gattcctcgt
     9301 ttgatcaatt cctgtaatgt ccgaatgact gaggcagcca aggaaatctg ggattgctca
     9361 ccaatgatct gaatgatttc tccacgtgta tggatggttg tttccgttgt ttcctcgatc
     9421 agttttatat gcttgtcgtg tgcacctaaa agcatgctga catcatcagc agctgtcaat
     9481 ctaatctcta aagaactaga ctcttccgtc aaaaatagac atcccctttt tttcgattca
     9541 ttctttccta ttttaccata ctcagaagaa actatatagc aaataagaaa aggttgtaaa
     9601 agagtcgctt aaccatacgt accaagaaac gttaaaaaat agccgtttcc attcgctaaa
     9661 ggtctcgttt tgataaaaag aagctgagaa tgtaagttat atgaaagtgg agaacaaaat
     9721 atttcgtttc attcaacaag tattctctag gtatttttcc tgtttcaaaa aaagaaaaca
     9781 aaatagttaa aatattcttg catttcaaaa aaaacaggtg tattattacg tcaacaaata
     9841 aacatacagc aaagcgtcga ggaaaacagt aggctattca acgatttatt cccagtgagt
     9901 catgtaagtg agaaatgaca ataaatcgaa tagacgaaca tcatttccga gtcaatgtcg
     9961 aaaacgtaga tcattgtggc tgcctccatt atcaggctag cggataactt tatcaaaaag
    10021 cgtccgtgaa tgaagaagct tttttaagcg aaaaaaggtg gtaccgttct agaatctaga
    10081 gcccttttcc caagtggaaa agggcttttt tttattttaa ggtggtggtt ttcatggaat
    10141 ctgatattag tggcattact aattgctgca acgtttatat cgtcaatcaa aatgataatt
    10201 attggaggaa aaatttatgg aagaaacaaa aacttattca tggaaacaac tattgatggt
    10261 cagttcactt atcttcggga tgttcttcgg gtcaggtaat ctaatctttc ctgtccactt
    10321 aggtcaaatg gctggcgcaa actgggtcag tgctggcgta ggatttgcta tctcaggtgc
    10381 tcttttccca ttactagcta tcttagctgt agtagtgaca aaaagtgatg gtctgtatga
    10441 cttagctaaa cctgtcggaa aatggtatgc tgccttgttc ttaatccttg ttcatttgac
    10501 gatcggacca tttttcggta caccacggac agctgctact gcttttgaaa tggctgctaa
    10561 accatttttg cctgaaaaat ataattcatt aggtatgttt ttattctctg ctcttttctt
    10621 tttacttgct tatttcctta ctgtccatcc aacacggttg gtcaaatacg tcggaaagta
    10681 tctaaactcc ttgtttcttg cattattagc aatcattttt gtcgtttctt tattccatcc
    10741 aatgggaaat ctaaatcaat ctgctgcaga tacttatcaa acaaacgctt taacaaatgg
    10801 tgtcctagaa ggttataaca cggttgatgc agtagctttg cttgccttaa gtgtcacttt
    10861 tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa gtcacagaat taacggcaaa
    10921 agctggtagc ctgagcgttg cgctagaagt catgatctat tttggacttg ttcttctcgg
    10981 tgcgatgagt ttgaataaga tgtctttatc agaaaatggc ggaactgcac tttctcagat
    11041 cactaatttt tatttaggta acttcggcgc tagcttctta ggagtgatgg tcacattagg
    11101 tgtcttcact accgctatgg gactcgttgt ttcttttgcg caagatttcc ataaactgtt
    11161 ccctaaagtc agttatatga catggctgcg gttaacgact ttcgtctctt ttgtcgttgc
    11221 aaatgctggt ttagacaata tcatccaatg gtctttacca gtattgatgc ttttgtatcc
    11281 attgtcgctt gcgttgatct tgctttcctt aacagcaaaa tttttccaaa agacaccatt
    11341 tgtctatcaa gtaacgatgt tgtttgctgc tgttcctgcc gtattggata tgctggcaaa
    11401 ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc atgctcgaat tttatcatca
    11461 tcacgttcct tttgctgcat taggacttgg ctggatggtc ccaacgttac ttggttacgc
    11521 tggcagccta ctgttttatt acgcctatcg cctatcaggg tataaacaag aagcaaatga
    11581 actaccagaa gaataaccaa atcaaacaca atttaacata agaaatatta aacaggaaaa
    11641 aaccagctag atgaaagaat cctattcttt cgtctagcta ttttttagtt ctctttttta
    11701 cgcgcacaaa aaaactgcgg acaatgattg tccgcagttt aaatgggctc tataatatct
    11761 agtgttggtg acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga
    11821 caccttgaaa tcctttagaa taaagtcgac gaaaaccatt caaaggagtg tttcaagatg
    11881 tcctattcag agtttaccaa agagttacta gatattctag acctaaatct tatctttcat
    11941 gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat gttttgtttt tgatggcaca
    12001 ttgacctatc aaccagagga atgcttccat tgtcactatc aaaataagca aacgatcatt
    12061 aaatggggct ggaaaaaagt atccatctta ttgaatgatg tcagtaacta caagaccatt
    12121 ctgcggatta acaaacagcg cttcaaatgt aaacactgtg gaaagacttt tttagcagaa
    12181 gattcagtta gtgatcgtcg ctgctccatt gctcgcagag tcaaacaagc cattcttgag
    12241 ctattaagcg aacctctttc tatgtctttg atcgcacgta tgaagcacat ttctccgacc
    12301 actgtcattc gaatacttcg cagccttcgg cctaaaaccg tttctttgaa ccagccgtta
    12361 ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa acgtctcggg agccatgagc
    12421 tttgtcatga tggacggcaa gacccatcaa ctgattgata ttgtggaaaa tcggcaacta
    12481 aatcctttac gcgactattt cttgcgctac cctagaaaag tccgtgaaca agtacgtcta
    12541 gtggtttcag atttctatac accctatcgc acattggtga aagagtgctt tccgaatgcc
    12601 cgaatcgtcg ccgatcgctt tcatattagc caacacatcg gtcgggcctt taccaaccac
    12661 cgaattcaag taatgaagac ctttaaaaaa agggatcgtc ggtcgaagca cttaaagaaa
    12721 tactggagac tacttcaaaa gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt
    12781 ccgtcatttc gagatcatct gacagaagca gagatagtcg atcggctatt atcttatgat
    12841 gactccttaa agcgaggcta cgaagtctac caggattttc tttccgctat cagaagacaa
    12901 gatgttcctg aattcgtcgc gctcctaaaa gaagactaca aggagttgcc agagcactac
    12961 caaccagtgt tcacgacctt caagaaatat cgaacagaaa tcaaacgagc gttgcgagtt
    13021 ccctattcca acggccctat agaatgtttg aacaaccata tcaaagtact aaaacgaatt
    13081 gcctatggct ttagaaactt tcaaaactac caagaacgaa tctttttata tcgaggaaaa
    13141 tattttaaga aaactaagaa cactacccaa ctgactaaag ctagaacaac aacacggcta
    13201 gacaagatag ctgtctagcc gtgtgagtat ctcgattatt ctattgaatt tcggctctat
    13261 actttttgat gttggtgaac ttcaccaaca tcttttttgt ataaaaaaat atagaaaaga
    13321 caccctgaaa tcctttagaa ttaatttggc taccaattca taggaggatt caagatgtct
    13381 catactcagc ttatcaaaga taccctaaat attctagacc taaatatcta tttcgaggaa
    13441 aattgtttaa ctaaagaaaa atacaaaggg caaatttgct tgatttacaa aggaactctt
    13501 ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg tcccctctag gatcattcgt
    13561 tgggggacga caaccgttcg gttgttactg aatgatgtgt ctgagtatcg cacttatctg
    13621 gagctgaaga aacaacgatt taaatgtaag tcttgccaaa gaacattcgt agcggatacc
    13681 tctgttgctg aaaaacactg tttcattagt caaaaagttc gttggtctgt cattgctcgc
    13741 ttaaaagaga atacttccat gacggaaatc gctcgtcaga aaaatatttc cacttcttcg
    13801 gtctatcgtg tcatgaagcg attctaccgc ccattgaatc ctttcaaaca gactttgcca
    13861 aaggtcttat gctttgatga attcaaatcg gttcgaagtg ttgccagtgc aatgagtttt
    13921 atcatgatgg acggacaaag ccatgcatta ttggatattg tcgaaaatcg acgcttgcct
    13981 tatttagaac gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaattgatt
    14041 gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa agcttttccc gaatgctcag
    14101 ttgatcatcg atcgtttcca catcgtccaa catattggca gaactttctt gaatcatcga
    14161 gtgaaagaaa ccacggtacg acttaaagga aattcccact ctcaacgagg gttagggaaa
    14221 aaattgaagc gctattggaa actcctacaa aaagaagaag caaagttaaa ctatgaaaaa
    14281 cgggtttggc gtgccagttt taaagattat ttgactgaaa gtgagattgc cgatcgactg
    14341 ttagctgctt gtcccaactt acgccaaggc tatcagttat accaagacat tctttatgcg
    14401 gtaaagaaaa gagatcagtc actatttgag gattgtttaa ctagagacat tagcggtctg
    14461 ccagaaacct atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat
    14521 gcgcttcact acagctattc aaacggacca ttggaatgct taaacaacca catcaaagta
    14581 ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt tcaaa
//
LOCUS       DAFGFZ010000071        14589 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000071 DAFGFZ010000000
VERSION     DAFGFZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.072"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(71..649)
                     /locus_tag="LR726_001881"
     CDS             complement(71..649)
                     /locus_tag="LR726_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6216967.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(726..1256)
                     /locus_tag="LR726_001882"
     CDS             complement(726..1256)
                     /locus_tag="LR726_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL6216968.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1260..2120)
                     /gene="rlmB"
                     /locus_tag="LR726_001883"
     CDS             complement(1260..2120)
                     /gene="rlmB"
                     /locus_tag="LR726_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL6216969.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2113..2508)
                     /locus_tag="LR726_001884"
     CDS             complement(2113..2508)
                     /locus_tag="LR726_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL6216970.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2505..3917)
                     /locus_tag="LR726_001885"
     CDS             complement(2505..3917)
                     /locus_tag="LR726_001885"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL6216971.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3914..4450)
                     /gene="cysE"
                     /locus_tag="LR726_001886"
     CDS             complement(3914..4450)
                     /gene="cysE"
                     /locus_tag="LR726_001886"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL6216972.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4536..4775)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4772..6232)
                     /locus_tag="LR726_001887"
     CDS             complement(4772..6232)
                     /locus_tag="LR726_001887"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL6216973.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6402..7559)
                     /locus_tag="LR726_001888"
     CDS             complement(6402..7559)
                     /locus_tag="LR726_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL6216974.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7593..8963)
                     /gene="radA"
                     /locus_tag="LR726_001889"
     CDS             complement(7593..8963)
                     /gene="radA"
                     /locus_tag="LR726_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL6216975.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9026..9553)
                     /locus_tag="LR726_001890"
     CDS             complement(9026..9553)
                     /locus_tag="LR726_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL6216976.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9783..10589
                     /gene="proB"
                     /locus_tag="LR726_001891"
     CDS             9783..10589
                     /gene="proB"
                     /locus_tag="LR726_001891"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL6216977.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10586..11833
                     /locus_tag="LR726_001892"
     CDS             10586..11833
                     /locus_tag="LR726_001892"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL6216978.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11909..12253
                     /locus_tag="LR726_001893"
     CDS             11909..12253
                     /locus_tag="LR726_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216979.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12370..12696)
                     /locus_tag="LR726_001894"
     CDS             complement(12370..12696)
                     /locus_tag="LR726_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216980.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13311..14513)
                     /locus_tag="LR726_001895"
     CDS             complement(13311..14513)
                     /locus_tag="LR726_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6216981.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4019 a         3199 c         2670 g         4701 t
ORIGIN      
        1 ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga
       61 tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc
      121 tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa
      181 aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt
      241 cgcaattttc gattgcttct tttaataaaa gatgctgaat tgctaaaggt tcttgcaatg
      301 tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat
      361 ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc
      421 ttataacgct tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca
      481 atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta
      541 aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa
      601 atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc
      661 tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg
      721 atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc
      781 tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg
      841 gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat
      901 gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt
      961 gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct
     1021 tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact
     1081 agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa
     1141 cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt
     1201 tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc
     1261 tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca
     1321 tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc
     1381 aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca
     1441 ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct
     1501 ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact
     1561 gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg
     1621 atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga
     1681 tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca
     1741 atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc
     1801 acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct
     1861 tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga
     1921 aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg
     1981 aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc
     2041 tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg
     2101 tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc
     2161 ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt
     2221 tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct
     2281 tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg
     2341 aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt
     2401 gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc
     2461 tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc
     2521 catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga
     2581 atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat
     2641 tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc
     2701 cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct
     2761 agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta
     2821 aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta
     2881 tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac
     2941 gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg
     3001 atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt
     3061 ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct
     3121 tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc
     3181 gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca
     3241 tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa
     3301 ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc
     3361 cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt
     3421 gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta
     3481 cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact
     3541 tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggt
     3601 tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct
     3661 ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa
     3721 ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa
     3781 gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca
     3841 tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta
     3901 ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg
     3961 ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac
     4021 cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca
     4081 tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg
     4141 ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc
     4201 ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac
     4261 cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat
     4321 ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga
     4381 tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa
     4441 accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat
     4501 aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc
     4561 cacttttctt cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc
     4621 tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca
     4681 gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga
     4741 tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta
     4801 atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc
     4861 ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt
     4921 gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct
     4981 gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg
     5041 cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag
     5101 catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt
     5161 catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa
     5221 atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat
     5281 cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt
     5341 gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca
     5401 ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt
     5461 cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg
     5521 ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat
     5581 ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa
     5641 aattataagt tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg atattatcgg
     5701 attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca
     5761 cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg
     5821 tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc
     5881 gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca
     5941 gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt
     6001 ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact
     6061 ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa
     6121 agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa
     6181 tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc
     6241 tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa
     6301 tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc
     6361 caactggttg aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc
     6421 tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag
     6481 atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc
     6541 atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct
     6601 tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg
     6661 actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca
     6721 gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat
     6781 ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca
     6841 gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca
     6901 cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg
     6961 atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg
     7021 atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac
     7081 agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa
     7141 actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg
     7201 atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg
     7261 gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga
     7321 taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt
     7381 ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat
     7441 gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat
     7501 gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc
     7561 gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca
     7621 gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg
     7681 tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca
     7741 atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt
     7801 ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc
     7861 gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga
     7921 agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga
     7981 cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct
     8041 accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct
     8101 aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc
     8161 atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc
     8221 cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac
     8281 attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc
     8341 ccatttgttt tagcggtttt gagtagttct gcagtggttt ctcttacttg gctgacactt
     8401 cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa
     8461 tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga
     8521 taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt
     8581 tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt
     8641 aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc
     8701 accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct
     8761 tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc
     8821 cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg
     8881 caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat
     8941 tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa
     9001 atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt
     9061 gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac
     9121 gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat
     9181 ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca
     9241 acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt
     9301 ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt
     9361 ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt
     9421 tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat
     9481 gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc
     9541 ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac atatccgagg
     9601 ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa gtaaaccatt
     9661 aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt tacatgactt
     9721 tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga ttgaggtgtc
     9781 acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga acaagtacat
     9841 tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct tttgtccttt
     9901 ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct gtaggcgttg
     9961 gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag caagctgcgg
    10021 cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt gtatataatc
    10081 aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa agtcgtaaaa
    10141 atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc gtgaacgaaa
    10201 acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat aatgatcagc
    10261 tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt tctgatatcg
    10321 acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat cgctatatcg
    10381 ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca tttggaacag
    10441 gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac agcgcaatga
    10501 ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga aaagaagtag
    10561 gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac aacaggcaaa
    10621 agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc tacttgttca
    10681 aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc ataaagactt
    10741 agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc agctgacacc
    10801 tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag acgatcctat
    10861 tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca aaaagcgcgt
    10921 gccactaggc gtgatcggca tcatttacga atctcgtcca aatgtcacga cagatgcagc
    10981 tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag aagcattctt
    11041 ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg gcgaatctcc
    11101 ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt tgatgcgatt
    11161 gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc aacgagtcaa
    11221 agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg tatatatcga
    11281 taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag cttcccgtcc
    11341 atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac cttttttctt
    11401 gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg atgctcgtgc
    11461 gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata ctgaatatct
    11521 agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg cacatatcaa
    11581 tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg ctagtcaacg
    11641 tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc gttttacaga
    11701 cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt tgcatgcccg
    11761 tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg gcgatggaca
    11821 aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt tttctgtaaa
    11881 ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt tttttattta
    11941 ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc ttttctttca
    12001 attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt ttgacaaaat
    12061 cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat tatgcttatt
    12121 agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga ttacgctact
    12181 gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc acttgaatcg
    12241 gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc ggcaaaaaac
    12301 tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt ccatataaac
    12361 ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa gcgaaaatcc
    12421 gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt tcatcgtttt
    12481 ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct gatacatacc
    12541 agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta ttggcaattc
    12601 tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta attcaacgag
    12661 tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca ttctttttct
    12721 taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg agaaaaaaag
    12781 ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt tcttctgcca
    12841 actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt tgaaattgtt
    12901 ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc gttcaatcag
    12961 ctgaccctcg gtattagctc aaaaaacggt aaaatatgag aagctatttt tccgttttgt
    13021 ttctctaata cttaggataa aacgctgatt ttacaacctc tgttaaacgg tgttcaatca
    13081 gctgtccttc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgtttgt
    13141 ttctctaata ctcaggacaa aacgctgatt ttacaacctc ctgataaacg gtgttcaaaa
    13201 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta tttttgtttg
    13261 gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc ttattcttct
    13321 tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat aagaacaatg
    13381 gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac acctgcaata
    13441 aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga agcaacgttt
    13501 cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat aattccttgg
    13561 aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt tccagctcct
    13621 aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg ataagtcgta
    13681 aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag cacaccccag
    13741 ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa tggcgcatac
    13801 gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac tgaacgaagt
    13861 ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt tggtttctca
    13921 gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa tgctacccca
    13981 aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg gccaacagcc
    14041 attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt accaactaaa
    14101 atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac accccagccg
    14161 gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc agcgaataca
    14221 acgatgataa acaatcctgt catcaatgtt tttttcaaac cgatccgact agataagaaa
    14281 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    14341 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    14401 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    14461 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    14521 tctctattag catcatacat gatttatttt acatgtcaat atgcacatgt aaaacgtgga
    14581 aactttact
//
LOCUS       DAFGFZ010000072        14526 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000072 DAFGFZ010000000
VERSION     DAFGFZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.073"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..623)
                     /locus_tag="LR726_001896"
     CDS             complement(<1..623)
                     /locus_tag="LR726_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL6216982.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDL"
     gene            complement(871..1707)
                     /locus_tag="LR726_001897"
     CDS             complement(871..1707)
                     /locus_tag="LR726_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL6216983.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1718..2647)
                     /locus_tag="LR726_001898"
     CDS             complement(1718..2647)
                     /locus_tag="LR726_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL6216984.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2839..3192
                     /locus_tag="LR726_001899"
     CDS             2839..3192
                     /locus_tag="LR726_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL6216985.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3266..3922)
                     /locus_tag="LR726_001900"
     CDS             complement(3266..3922)
                     /locus_tag="LR726_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL6216986.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4148..6082
                     /locus_tag="LR726_001901"
     CDS             4148..6082
                     /locus_tag="LR726_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL6216987.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6168..7391)
                     /locus_tag="LR726_001902"
     CDS             complement(6168..7391)
                     /locus_tag="LR726_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL6216988.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7385..9283)
                     /locus_tag="LR726_001903"
     CDS             complement(7385..9283)
                     /locus_tag="LR726_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL6216989.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9277..10395)
                     /locus_tag="LR726_001904"
     CDS             complement(9277..10395)
                     /locus_tag="LR726_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL6216990.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10478..11311)
                     /locus_tag="LR726_001905"
     CDS             complement(10478..11311)
                     /locus_tag="LR726_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6216991.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11304..12221)
                     /locus_tag="LR726_001906"
     CDS             complement(11304..12221)
                     /locus_tag="LR726_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6216992.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12244..12423)
                     /locus_tag="LR726_001907"
     CDS             complement(12244..12423)
                     /locus_tag="LR726_001907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6216993.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12448..13572)
                     /locus_tag="LR726_001908"
     CDS             complement(12448..13572)
                     /locus_tag="LR726_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL6216994.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13755..>14526
                     /locus_tag="LR726_001909"
     CDS             13755..>14526
                     /locus_tag="LR726_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6216995.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAK"
BASE COUNT         4585 a         2872 c         2340 g         4729 t
ORIGIN      
        1 tttaaatctt tacataaata ctcatattcg agtgattttt tatatttttt aattgtgccg
       61 tttacgagaa tctcttcatc tttcaatact aggcctattt tatttttata acctggcatg
      121 ctcattagat aaaggatttc atcgtaatgg atatagtgat cgctattacc ataacggaca
      181 gttgtccacc tttttttcgt agcatgttga acaggatagt tttctttttc tttcaataag
      241 taactttgga tgcttttttt atttttttca taagtgaatt cttttatcaa ataaccacta
      301 ggcgtaatct ttcgattaat aacagttatt gctttattcg gatgagacgt tagataaaca
      361 attttacact tttcattctt ttctctaagc atttcgccta gttcgatacc gctataatag
      421 gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat agatttcatt atttttaatg
      481 aattcaacaa attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat
      541 aaaattctgg attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc
      601 aaaataaata tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc
      661 atttcataca acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt
      721 ttccaacacc tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat
      781 caaacaaatg aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct
      841 gtctgagcta tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt
      901 cggagcagag actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg
      961 ttgataatag tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt
     1021 tactccttcc ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat
     1081 aatgccgaat cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa
     1141 tccttcatag ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat
     1201 aacttctaaa tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt
     1261 caactcattc atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt
     1321 taagtattgt gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa
     1381 tcctttggct tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat
     1441 tactggcaaa gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg
     1501 gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag
     1561 ctgaactaaa gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg
     1621 gacattgata tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata
     1681 cccagataaa atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa
     1741 taacgctaag ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt
     1801 tcatcatcag tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag
     1861 gtgctgtgcc gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa
     1921 gagacatcgt aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga
     1981 gcgttagcat aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa
     2041 tgatcaaata acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag
     2101 tttgatggat cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat
     2161 gatgatggtg attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt
     2221 gaaaataggc aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc
     2281 agcttgccat ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat
     2341 tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa
     2401 caaattgatg aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga
     2461 tcgaaccagt aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg
     2521 gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg
     2581 cttcaataac gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag
     2641 aaaacatgag tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta
     2701 ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact cttatttttt accaaagcac
     2761 ttcaattgac tttttctgtt attcccatta aattaaggtc tcatacatat cacttctatc
     2821 ataaaaggga gaataaaaat gaaacaaacc actaacttga ctgaagttca agacatactg
     2881 caacagtcat ccgttgccgt catttatctt tcgatgccta attgcagtgt ttgtcacgct
     2941 gttcgtccac gactagagga acttctcaca cattatgata ttccggcact tcatttggat
     3001 gcatttgaaa caccagaagt agctagtaaa tttgaagtgc tgactgcgcc agttgtcctg
     3061 atttttcatc aaggaaaaga agtctttcgg caagcacggt tcatcgactt taaaaaaatc
     3121 cgttatctac tagatgaact gacagcagaa gatgattccc tttcttatga agaaatcttc
     3181 cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa agttttgcct
     3241 tcatttgttc aatcgctttt tatttttatt ctgctgtatt ttcggaatgc gcatttgcgt
     3301 cagaatgcaa gaaatagatc aaggtttgca attcattcgt caaatcaaca ttttggatca
     3361 atacatctga cggagcattc aatcgagcag cggtaaagtt caagattcct ttgattcctg
     3421 cttccgttaa ctgattgact acttcttgtg ctctttcagc gggtatcgtt aaaattgcta
     3481 cttcgatttg ttggatcttg atttgttctg acatatcttc cattggataa acggggatac
     3541 cgtctacgat gcgcccaacg atttcctcat tcacatcaaa agcacaactg atacgtatgc
     3601 tattactttg atggaatttg tattttagta aagcacttcc caagttccct acaccgatca
     3661 gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc ataagatctt
     3721 ctacatcata accataccca cgttttccta attcgccaaa ataagaaaaa tctcgacgga
     3781 tcgttgcact atctacttga acggcttcac ttaattcagt tgaagacact tttttctttc
     3841 cagcattatt taaaatcctt aaatagcgat aatacaaagg cagtctttta gctgtcgcct
     3901 ttggaataat atgatctttc acgaaatgac ctccacgtat tgtctaatta catctttgat
     3961 ttctccatca tagcagtatt tataggaaaa aatcaattcc attgctcaga tattgtgaaa
     4021 ttttattctt tattcattta tttatcattt ctgcaaatct cgcagtataa tggaatttat
     4081 ggtagactac taattgaaaa atagtttttt atttgaggag aattcacaaa gtagaaaaga
     4141 ggattccatg atattattac aagccaatca gattgctcgc cattttggtg cagaagtctt
     4201 atttgaaaac atacatttag aaatcagtac aggcgctcgt gtcgctttag ttggcagaaa
     4261 tggcgctgga aaatcgactt tattaaagat cattgctgga attgaagcac ctgatgaagg
     4321 aaagatcgct aaaaataaaa cagcgactct cggatacctc gctcaggaca caggacttga
     4381 ttccaatgaa acagtttgga acgagatgct gaaagctttt gaacaagtta gacaaatgga
     4441 agagcggatg cgcgagctag agattttcat tagcgaaggt acgccagaca cacctgctta
     4501 tagttcttat ttaaaagaat acgaccggct gcaacacgat ttttcagatc gaaacggcta
     4561 tggttatgaa aatgaaattc gttccgtatt gcatgggttt aaattcgatt cttcatttta
     4621 tgatcagaaa atcgatactt tatcaggcgg acagcgcaca cgtttagctt tagctcgcat
     4681 gcttttgcaa caccctgaca ttttgattct agacgaacca acgaaccatt tagatatcga
     4741 gacactttcc tggcttgaga attatctgca aagctatact ggtgcattat tgattgtttc
     4801 ccatgaccgt tattttttag ataaagtagt caatgaagtc tatgaattaa gcagaagaaa
     4861 aatgcgccat tacaaaggta actattcgaa atatctagat ttgaaagcag aacagttagc
     4921 aagtgaatgg aaggcttatg aaaagcagca aacagaaatc cataaattag aagacttcgt
     4981 tgctcggaac cttgttcgcg cctctaccac aaaacgagcg caaagccgca gaaagacatt
     5041 agaaaaaatg gaccggctgg atcgtcctca aggggatgaa aaacaagcaa actttctatt
     5101 cagtatagaa aagccttctg gaaatgtagt cttgcaaatc gaagatgccg ctatcggata
     5161 tgataatgtc accttatctg aaccaatcaa tctagatatc cgtcgtcaag aagcgattgc
     5221 tctagtaggt ccgaatggta tcgggaaatc gacgttgctg aaatcaatca ttcaccagat
     5281 cccttttatc cgtggaaaag aaacgctagg gacaaatgtc accatcggct attacgatca
     5341 aggtcaagct gatctgcata caaacaagac gatcctagca gaattgtggg atgagcatcc
     5401 taccactcca gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt ctggcgaaga
     5461 tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg ctttagccaa
     5521 gttagcgatg aacaaagaaa attttttgat tttggatgaa ccaacaaacc atttagatat
     5581 tgacaacaaa gaagtattag aaaatgcatt gatcgattat gacggcacac tgctttttgt
     5641 ttcccatgac cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac tctctgaaaa
     5701 tggcagcaaa gtctatctag gagactatga ttattattta gagaaaaagc aggaagaaga
     5761 agaaatcgcc gaattgttgg cacaagatga gccagaatct gctcctgctc ccacgaacaa
     5821 atctgattat taccaaagca aagaacagca aaagcttatc cgtagccttc aacgaaaaat
     5881 tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt tggaacaaga
     5941 aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc aatgagctga accaagattt
     6001 agaagctgct agacaagatc aagaaacaaa attgacggaa tgggaagaac taagcttgga
     6061 attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat gctgttttgg
     6121 tttttctttt tttattttat caaacaaaaa ttccatattt acctttttca ccaataagaa
     6181 gaccaattac tagtcattct cactaaagct tctaaataat agtaatcacc ccaaatacaa
     6241 ggtgtattta ccccattatt tttgggctta ttataaacag actgtgttaa aaagccatct
     6301 tcttcttccg ttgtagcata tctttctttc aattgtcgca taattttcca aacagcagac
     6361 tggtataagt tcttttctgt tttgtctaat agacctgatt ctattaattc taataatcca
     6421 caaaccatga tcaccatact agatgtatct ttttcttctc tgctagaatc atcaaagatc
     6481 aaatcccaat aacaaatggt atcttctggc aaccgattca taaaataatt ggctaatatt
     6541 ttgcttaaat caaaatattt ttgttctttc aaataacgat aacttaatgc aaaaccgtaa
     6601 atcccccatg cttggcctct agcccaacag gaattatcac tataaccttg atgcgtactt
     6661 ccatatcttg gttttccatc tttgacatcc atataaaatg tatgataagt tgaaccatct
     6721 tctcttacta aataatctgc tgcattttga atatgagcct gagcagcttt tttatatttt
     6781 tgatttccag tttcttcact cgcccaatac aataatggaa gattcatgca acaatccatg
     6841 atcattctac cttgttgtga aggatcacta ggatctcccc aagcttgtat aactcctgcc
     6901 ttctcccaaa aacgctgaag taatgtttct gcagcatcaa ttgaagcttc cttcattttt
     6961 cgatttcccg ttattttata atctgctacg gttgataaag aatacaaaaa accaagatcg
     7021 tgatgattag tgataatttg acagtcaatc ctgtggataa aatctatatc tgctttttca
     7081 gctgtttgtt gaaataaagg atcacccgtc ttctcaaata acaaccaatt aattcctgtc
     7141 caaaatcctt ctgtccaacc aatattgtct cttaatggat aacgtccttt tataggacac
     7201 tcgcttggga attgatactg actgttactg atacgtttga ttaacctctc cgtgtgtctg
     7261 cgacaacgct cgatctcatt attccattct ttttccatta attctttata acattcattt
     7321 atccattttt ctggatgtat tatctcttca aacatcactt tatcaagcat ttccccacca
     7381 gcctttagca taattttagt ctttttttat tgtttttatt ttggatagtt agcccggttg
     7441 tcccatcaaa atcaattttg ggacgattgt tccatacttc taaactataa tcccgatctt
     7501 gatgagcaac cactcctgtt ccgagccaat gggttccttt tccaaatgta ccctctaatc
     7561 caggaataaa agtgttttcc catgtcatca agtttgtatt gggcaaaccc ataatagctg
     7621 tggattttcg tttaatagta tcgtcactta aacagataat acctgaaaag ccagcttcac
     7681 tcaccctata gttttccttg tcttcttcta tgattattcc ttctttctca ggagaacatt
     7741 caatagcaaa ccctgtctca tatagaacat attctgtatc taattctact ttgtgtacgc
     7801 gaatctgcca gccttcaaaa ggaataagcc aagttctgat ttttgcctgt aaagtaggtt
     7861 cccatgtaga ggcaagtacc tctttcttac atacaatgtc tcgtacatta tttcgaacga
     7921 tgaactgtcc ataggttttg atgctctcat tcttaaaact ggtcgtgata tatgtgtccg
     7981 ctcgctgaat ccctagcatt gaatccattg ccatttctgc tttcaattga ttcgctcgcg
     8041 gtatcgaaaa accaaattga ctagaataag caaattttaa atatttttca ttggtcaatg
     8101 tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca ttatcatgtt
     8161 gtatgagcaa attggcttct gaaagaagtt tagttccttc acgaataggc ataggcattt
     8221 ctttagccgt ccaatactca tggtcttcag gtagcgccaa caataaaaaa atcttattca
     8281 gccataatgg agataacgta ctattatagg gttcagtcaa tgtcaattga gggtaggtat
     8341 aaccaatatt caggatacct ttttcatcaa atatttcttt ttgcatccac catcttatat
     8401 tccgattgac taaccccttg atttcaccta atttaaaagg caaaagatcg gcccacaaca
     8461 tagctgacca aaagcttgaa actgcaaaac gataaatcat gcttcgacca aaaggcacat
     8521 ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga aaaatcaata gctcggttct
     8581 tatatatctg gctaatgggc ttttctggtt ctagtacgct ataaatcaaa ccataataat
     8641 gaaatgcaaa aggattataa taatcttggc gacctcttga actgtctcga taccagccat
     8701 tttgaagata acaattttct atattatcaa atgcttcttt cagtgctgat tctttaactg
     8761 gataaccaaa tttttggata attgttccga caattatctt aaaaaattgc caattaccat
     8821 ctgcacattc atattcaaaa atttgcgaga accaatgaat aatctggctt ttttctcctg
     8881 tatctaattt tttccataat aattgttcat gatgatataa cgctaataac attgcgggca
     8941 tttctaccat acgctggtct ctttcatgta tttctcccca ataatcggga tgggaaggat
     9001 ctgttccagc ttttattcct gatagaatta atttcaacca tcgttcatct aattggtcaa
     9061 ttgcgggccc cgctccccat aaaacccgag agaatccttc cattcctgca attttatttc
     9121 catatcctac cccttgatgt atataatcaa tttgtgcgcc acttgctcga taaaacggct
     9181 ggagtggttg atttatcttt ttaaataagt caaagtaatc ctgtctggtc cttaatagtg
     9241 gtactggatg tttcattgta ttttctcctc tttttactac caaatagcta attccgactg
     9301 ttctaatcga agtaagcctt caataaaaaa ataatcagaa tagatcagtg gcacattata
     9361 agtcagttct ctatggtatg cttctgctcc gcctccaatg atcccatctt catccatatt
     9421 ccaatttgca tagtgctttt ctgtcttttg gagtatattg atagcagctt catgatacat
     9481 ttttgatcgt acagaggaaa catatcctgc taattccaga agaccgcatg cagcgcatac
     9541 tccagcactg gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag gaatataacc
     9601 agttttttcg attttctgaa taaaatttac tgctatcttt tctgctatag ctagatagta
     9661 cggatttttt gtgtaacgat aacttaacgc gaatccatat aaagcccatg catttcctct
     9721 gctccaacaa gaattctcat tgtatccttg cccgccaagt aaccgaatat atttccctga
     9781 ttctgcatca aattctgcga tatgcccggt tgatccatct ttcctcacta gatacttagc
     9841 tgttgtatct gcatgctgcc gagcaatttt agagaacctc ggatcaccag tttccctcga
     9901 agcccaatac agtaatggta aattcatcat actatcaata atcatccaac caggtttatg
     9961 gtcattccat gctactaaat agttccctga aatattatag cgtccagcaa gtaccgtagc
    10021 tgcatgtaac cctagttgtc gactatgttt gttttgagac tgttgataat gtgcaatagc
    10081 cgatggtaag aataaaaaac ctacatcatg atgaacgtct gaaaaatggt acagggcaga
    10141 ttctaataat tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt ttgtcttgtg
    10201 aaagagctgc cataaaatac cactccaaaa accattggtc caccagtcga ttcctctgtc
    10261 ctttattacg tctgtatatt ttccgttgta agaaatatat ggaattttat tccccattct
    10321 gtctaattcc gcatctatct tttcagaagc tacattcaaa atttttcttg accattcctt
    10381 cgtctgttca ttcatggata tcccgccatt cttttaattt ttatgaatgt ggagttgctc
    10441 ctagaacgaa ctagatggtt aacattcgtt atttttttta cttatttgca gctccaatgt
    10501 cattctcctt aaatttagac tgcgcccaca cataaatcaa gaccattggg atcactgaag
    10561 ttgctaaaat tagcataatt gcgccataat taattggctc acctgtacta gaaaccgtca
    10621 tatttttgat aattaaggga accgtatatt tatctttatc tgaaagcatg actaatggaa
    10681 atagatattg attccaactt cccataaaga taagtaaacc actagtaata atcgtaggtg
    10741 ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc attggcacca tcgattctgg
    10801 cagcctccat cagtgctgta ggaaattgac tgctatattg tcgcatcata aacactgaag
    10861 ttgtactggc aatcgaaggg atgatcacac ctgcataagt attcgataat cctaaacgac
    10921 tcataatgta aaataaagga atcaatgtgg taaaaaaagg caccatccta gatagcataa
    10981 ctgtattgaa caacagttta ttccccataa aacgatactt tgctaatgca taacccgcca
    11041 ttgtagaact ggctgtagct gaaactgtac tgattacagc aataacaatc gaattccaaa
    11101 taatcaaggg ataattatat ctcgccatga tggcttgcca gttttttaat agcatatccc
    11161 caaacttgaa attgattccg ccacttaaaa tttcttgatt actttgcgtt gaagctacaa
    11221 tcatatataa atatggcatc aaaaatatta aggcaccaaa cacaataatg acgtacgtaa
    11281 aaattctgct ttgtatcttt tgtttagtca tttttatcac ctccgaattt aaattgaata
    11341 gccgataata taacagatac aaacaccatc gtccaggcaa tagcagaggc ttttccaaaa
    11401 tcaaccattt caaaacttgt tttatataaa ttgactccta cgaccaaagc tgaattttct
    11461 ggtccattgg aagtcccaaa gaatatattt gggatattga aattacccaa gccattgata
    11521 gtggctaaaa ctgtggccat taaaatgatt ggctttagca tggggatcgt taataaaaag
    11581 aattgccgaa atttcgatgc tccatctatt tcagcacttt cgtatatctc tttagaaatc
    11641 ccttgaagac cagctaaaaa taaaatagtg ttatatccta cactaagcca aatcatcgtg
    11701 atgatcattg atattcgtat ccaaaaagga tttgttaacc actgtacacc gttaattcct
    11761 agcgcattca aaaatccatt gaataaccca gtcggattga aaaaagtcat gaatacggaa
    11821 gctacagcca ccgttgatgt aatggttgga atataataga cagttctaaa aaatcctttt
    11881 ccttttaact gtgctgaatt caataaaaca gcaaagtaaa tagctaatag catcgttatt
    11941 ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa caatggatct
    12001 cggaagatag ctagataatt gtcaaatcca acaaattcaa atgagtagcc aattttcata
    12061 aaactaaaag caaacgagat aatgatggga atcaaaatcg tcacaataaa gaaaataaca
    12121 aaaggtgtga ccaatccata agcacttttt ttatgagacg tatctgttgc ttttttcttg
    12181 ctgttcttta ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc ctcctaatat
    12241 tttttaatca ccagttaatt ttacgccgta cttatgagca aaattttctg cttcttttag
    12301 atatgaattt tcaaaatcac ctgtttgcca atagttatat gcagcggtag tcaaataaga
    12361 cgtagcatct gaataagtgg ctggtaaaac agtatttgat gtattttcat tgattttatt
    12421 caatagttca tagtattttt gtcctcctta gtagtcaaac tttttctcag ctgcttctgt
    12481 ttgataggcc aatgagttag atgtagccac gcccagctcc agtgcgttgg ctaagcattc
    12541 ttcatctgtc agcataaatg taagaaattg ttgtgccact tgtggatttt tggcatcttt
    12601 agggacataa aaacttgatc ctcctgatac tgcacctttt cctggatttt cttttgaaaa
    12661 aggaatcagt ggagccatgc gccatttgtc agatgcatca ggaaaattca aagacatatt
    12721 cgtagcatac cagccgccag caacaaacat tgcagattct tgaaatgctt tctcagcatc
    12781 ttgtgcaccg tagaaatgta caatatctga gtcgatcatt tctttgatta actttgcagc
    12841 atgtttcgct tctttagtat tcagacggat atttccttct tcatcaagca aagattgatt
    12901 ctgctgggct agcatgttga taaacaattc ttggtcccca gattctggca aggcaattcc
    12961 aaaactacca gttttttctt ttaatgcttt tgccacctgg atatattctt cccaagaact
    13021 gatttcttca aaatctacac ctatttcagc aaattttttt ggttgataat aacaaagtgc
    13081 tggggtaaaa tcactagccc aaggaataat cgtttcatta ctcgtttgtt gacgcaaaat
    13141 attcaatttt tgatcataaa actgatcgcc atatttgtca taaaatccat attcatcagc
    13201 actataaaaa gcatcttgaa atttttccag cacacctgct gctgaagtgt ctgccatgga
    13261 gataatatcc ggcatatctt cgtgtgttac caattttggg gtgatcattt gtactgttgc
    13321 tgttcccgaa gctacattca caatttttaa gtcaatattt gctccagttt tgcggttata
    13381 gatttttgca gcattttcaa catagtttgt cagtttgccg tttgcgacat atacttctaa
    13441 tgtagtggct ttttttatca ttggatctgc gatgtttgct gttacttctt cgttagtttt
    13501 tccacatgca cttaagctga ataatagggt aatgtttagc aaagctatca ccaatttgta
    13561 tctttttttc atgtctatcc cttctttctt ttcaagttct tatataaaac gctttcactt
    13621 atgtatatta acggaattag taacacaaat atttatataa aaaaatattt tacttgtcgt
    13681 tttggctttt catttttgtt ataatagtca ttaattataa atagaaagct ttttatataa
    13741 aaagagaaag gaaagtggtt cttttgttaa caaacaacca atcctccaat cttgtcaaaa
    13801 aaacactaac ctattccata gatacccatc ttcatttatc tgctgctccc tattttcgac
    13861 tactaataaa tgaaagcgct aatttatatg ctgaatttgc acataaatct atagaactag
    13921 ataatccttc ccttttattc attgataaac agcaagaagt ccaaatcttt tctactcaaa
    13981 aggaaacaca aacttttatc attatccagt tttattttga tgagcaatta ttagcacata
    14041 ttacgggaca atctttttct atagatcaag aaataacatc tatcattact tttctgaaaa
    14101 atgaacagtt caaaattgat tatctaaaac atagtaagcc agctatttcc tatgatcagg
    14161 aaatcaattc ttttttcata ctatgccaac agatgagcat tcatttgctt tccttattag
    14221 ttacttattg tctgcaaaaa attcaattgt cttccttacc aaggcattat caacaggaac
    14281 ttgctttatt gacaatacct tcaaatgaga aagtcattcc taaaaaaaag catattcttt
    14341 attcagatga attagttgat ccaatcatac gatatttaga agaacatctt tcagaaaatt
    14401 ttttgcttga acaattagcc caaatttttt ttatcagtcc agccagctta aaaaaacatt
    14461 ttagaaaagt gaatggaaaa agtgttatta gctattttaa agaactaaaa atggaaaaag
    14521 caaaac
//
LOCUS       DAFGFZ010000073        14305 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000073 DAFGFZ010000000
VERSION     DAFGFZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.074"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..717)
                     /locus_tag="LR726_001910"
     CDS             complement(<1..717)
                     /locus_tag="LR726_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL6216996.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELL"
     gene            complement(767..2263)
                     /locus_tag="LR726_001911"
     CDS             complement(767..2263)
                     /locus_tag="LR726_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL6216997.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2457..4079)
                     /locus_tag="LR726_001912"
     CDS             complement(2457..4079)
                     /locus_tag="LR726_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL6216998.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4407..5720)
                     /locus_tag="LR726_001913"
     CDS             complement(4407..5720)
                     /locus_tag="LR726_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL6216999.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(5965..6189)
                     /locus_tag="LR726_001914"
     CDS             complement(5965..6189)
                     /locus_tag="LR726_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217000.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(6209..6631)
                     /locus_tag="LR726_001915"
     CDS             complement(6209..6631)
                     /locus_tag="LR726_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL6217001.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(6773..7483)
                     /locus_tag="LR726_001916"
     CDS             complement(6773..7483)
                     /locus_tag="LR726_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217002.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(7501..7998)
                     /locus_tag="LR726_001917"
     CDS             complement(7501..7998)
                     /locus_tag="LR726_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217003.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(8035..8487)
                     /locus_tag="LR726_001918"
     CDS             complement(8035..8487)
                     /locus_tag="LR726_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL6217004.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(8622..9227)
                     /locus_tag="LR726_001919"
     CDS             complement(8622..9227)
                     /locus_tag="LR726_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL6217005.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(9677..10297)
                     /locus_tag="LR726_001920"
     CDS             complement(9677..10297)
                     /locus_tag="LR726_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL6217006.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(10299..11168)
                     /locus_tag="LR726_001921"
     CDS             complement(10299..11168)
                     /locus_tag="LR726_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL6217007.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(11191..11598)
                     /locus_tag="LR726_001922"
     CDS             complement(11191..11598)
                     /locus_tag="LR726_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL6217008.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(11617..12906)
                     /locus_tag="LR726_001923"
     CDS             complement(11617..12906)
                     /locus_tag="LR726_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217009.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            12964..14303
                     /locus_tag="LR726_001924"
     CDS             join(12964..13405,13405..14303)
                     /locus_tag="LR726_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL6217010.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         4295 a         2931 c         2362 g         4717 t
ORIGIN      
        1 taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc
       61 ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct tttggacagt
      121 cggcatgata ttggaaaaaa ttgtactgat ttgttgagga aaccttgcgt gttggatcac
      181 cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag caatgatttt
      241 tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga tccaacgcaa
      301 aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt ctgctatctt
      361 ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg aagctcggaa
      421 atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct gtttagtttc
      481 agattcaatt gattctaaaa aataacctga acgtttattt gactggataa tgattttctt
      541 ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt tggtacctaa
      601 agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata ctacaagaat
      661 ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta taaacataca
      721 ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat ttgaagaaag
      781 atctatattc aaggttttta ccattagact ggataatttg tttataatat tcaaaactat
      841 cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca taaataaagc
      901 catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt ccccaccata
      961 agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga agatgtattt
     1021 ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta
     1081 gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc aattcattca
     1141 tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga gatttttcct
     1201 tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt tctgcttctt
     1261 ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg ttagcttgca
     1321 agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc catgtcctgt
     1381 aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt
     1441 taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa gtcgctaatg
     1501 cagaaaagct acacataata cccatcttag catttggatc gattttcttt aataattgag
     1561 tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact ttttttgtta
     1621 gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca gcagctgcat
     1681 aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc actttccctt
     1741 ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt
     1801 gccacccccc atactctcct accaatgcta agggggtttc ataatgacta agagtaatta
     1861 ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat acgagaccag
     1921 cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag atgcttgttc
     1981 gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat cgatgataaa
     2041 aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc tcccaaatct
     2101 ttttttcttc attccactga atactaggat gctggttcat gatcccgata agcgtatctg
     2161 tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta gcagctactg
     2221 ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc tctcctcgct
     2281 atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat tctaactatt
     2341 tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt tatatataac
     2401 aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca aacgatttat
     2461 tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt
     2521 ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt gaacgtctgc
     2581 ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat gcgagaatag
     2641 gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac gatgatgtca
     2701 tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc ttgatctgct
     2761 aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca tggaattcca
     2821 gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc attgtctctt
     2881 cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga aatcctttcg
     2941 ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat tgggcaattc
     3001 gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat atggagcaag
     3061 atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca acgagaaaca
     3121 attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc acctaaagat
     3181 gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt caaatcccat
     3241 ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac catggataat
     3301 tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc ccatgtttcg
     3361 ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg aagatactca
     3421 tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat catatcgatc
     3481 acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat ttgataaatc
     3541 tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct atacaagtgc
     3601 agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa tattgagcgc
     3661 aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc ccgataagga
     3721 ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt atgattcacc
     3781 accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag ttcttccatt
     3841 tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc atacccattg
     3901 tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc taaatacgcc
     3961 agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg atttgaatct
     4021 tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt ttgttccatg
     4081 attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt tagtctctct
     4141 cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta acagaataaa
     4201 acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga tttatgcagg
     4261 gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt cccttggtat
     4321 tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc taatactcag
     4381 gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat gaatcccaca
     4441 aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt agctgctaac
     4501 atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact ataagtcaaa
     4561 ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt
     4621 tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc agttacttgt
     4681 gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa aatccctgta
     4741 gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga ttctacaaat
     4801 gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga atcagaagct
     4861 aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg gtttgctaat
     4921 ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag cactacgacc
     4981 cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa tgttggtttc
     5041 aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt gaccagacct
     5101 aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg aacaagcaag
     5161 atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct
     5221 aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag accaaggaaa
     5281 gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg gatcgcatca
     5341 atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga agcaacgaat
     5401 acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga ataagcaaaa
     5461 aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa tacccccatt
     5521 aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc ttcatccatt
     5581 ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga gataaatgtt
     5641 gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat ttcgaagtag
     5701 gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa ataaagtccg
     5761 ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa caaaacataa
     5821 gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt
     5881 atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa
     5941 gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg
     6001 ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc
     6061 tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc
     6121 tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat
     6181 tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt cctataacaa
     6241 aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta tctgtgattt
     6301 ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa aatactaaag
     6361 aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt gggaatacgc
     6421 ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga
     6481 ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt
     6541 cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc tttctatttg
     6601 attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt
     6661 ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaactt
     6721 tttttacaaa aaaaaaacgg caatgaatgc cgctcttctt atttcgattc ttttataatt
     6781 ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat
     6841 cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga
     6901 tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt
     6961 cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg
     7021 gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt
     7081 ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa
     7141 aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt
     7201 cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga
     7261 atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg
     7321 taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac
     7381 tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag
     7441 gaaatacttc ccagaaattg gtatccacta ttactttttt catctgttca cacctcttga
     7501 ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac
     7561 atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg
     7621 ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt
     7681 atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc
     7741 gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag
     7801 gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag
     7861 ctcttctgta agtttttcta gtgcataagc ttgtgtcaag taatcagcta aatgctccgg
     7921 tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc
     7981 tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata
     8041 gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt
     8101 atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc
     8161 attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg
     8221 atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga
     8281 ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag
     8341 tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat actgtcctct
     8401 tgttaaatcg acttctttga attcgatatt gctaatagaa tctagcgctc gggcaatcat
     8461 tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt gtaagactag
     8521 ttcacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt tcttttttaa
     8581 taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca atagatctgg
     8641 tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga tagctttggc
     8701 aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat aaacttggcc
     8761 tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag ctgtggaata
     8821 tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac tgttaatcct
     8881 gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat ttcagcaaat
     8941 ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac cttgccatct
     9001 tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc aatcgttgtg
     9061 agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc caatgaaatc
     9121 gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata tagaccagct
     9181 tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct cttcccttct
     9241 tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctggtttaat tacggcaaat
     9301 aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa caaaagacat
     9361 tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc ggtaataagc
     9421 cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt aattgaggca
     9481 ggacagcttt ttcacaacct ccccactaaa cggtgttctc ctaccaactc ctcggtcaca
     9541 agtcacaata ttcatcaaac atgagaagct gtttttttga aattgctcct tgtgactcgg
     9601 agctgaacga caggctcacg acctctcggt aaacagcgtt caaaagccaa tttttcgata
     9661 ttaagccatt tctttgttac ttattgtcat tgatttcttt ttctaattta tccaatagtg
     9721 gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt cctaactgga
     9781 atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta atatgattgg
     9841 tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg atatagctca
     9901 aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact tcaaagtctg
     9961 ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct tggagtcgtg
    10021 ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct gcttccatat
    10081 acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt tccgcccgtt
    10141 cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca tcgagcgtga
    10201 tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt tttcctgtac
    10261 caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc taagttaatt
    10321 ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt tactgcttca
    10381 tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact aaaggtttct
    10441 ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt gcggatcccg
    10501 ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg gataactagg
    10561 gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc tgtttttgat
    10621 ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc tgtagcttct
    10681 acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc gacatggatt
    10741 ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc gtgcgcatgg
    10801 gcatattcta ctactgcttt tgtcttcagg atattttctt tgaaaggcag atgggaccca
    10861 tcatacatga cagaagtata tccgctgtcg attgcttctt tgatatcatc aaagtctcgg
    10921 gcgtggtcta agtgcaagac tgcatcaacc atttcttctt ctgcaatagc tgtcattaca
    10981 ttgactaaca catgcatgcc gatatattta gcggtatcga cacttgtttg aataatgatc
    11041 ggcgaaccca tttcttttgc tgcgcctagc atagcaggaa gcatttctag gttatgcgta
    11101 ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc tttcaatgtt
    11161 gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg ttacttgttt
    11221 tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt gatcatcacc
    11281 agcacgagca gcatctgcac gcttctggtt atagaggctc atcagttttt tatacccatc
    11341 tccgatcgtc tgtttatttt ccaaactctt ttctaaatat tggatggctt gatcgttcac
    11401 atacaattca gaaaatttaa gtccaacttg ttcataaagt ttcgctaatt tatgcttatc
    11461 ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct tttcggtcgc
    11521 ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt tttctttttt
    11581 cttaaaaaat ccaaacatca ttttccctac tttcaattaa aatgctttca aaactgggct
    11641 taagatccag gcatataaga gtgctgcggc tactgtatct acatcggttg ctgtagcatt
    11701 gttgaatccc atcgaattaa agatcgttac tagcaaagcg ggcaataatg taataaagaa
    11761 accatgagca ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat
    11821 ccctgctgtc cctccggcaa agaaattggt tagcatacca ggtaaaatca tcgctaagcc
    11881 gaacatgggc aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa
    11941 aatgacagca tttgggctat aaggaaagaa aactggacaa tctaaggcag ggatcgcatc
    12001 aggaactaat ttcatagcta tccctcgaaa tgccggcacg atttcaccta acagtagacg
    12061 tacaccagct agcaatacat acacacccac tacgaattgg atggcttgta aaaaggcaaa
    12121 catgatatag ttttgatctc ctgataattc agaagcgaat ggttcgcctg caaataaaac
    12181 agtgatgata tatagcggga ccatcacgac catgacagac aaataagtat cttgcagaaa
    12241 ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt ttattttcgc ctttctcacc
    12301 aaaaagctta gcaacaccgg cttcaaataa gtacccgatc gtacaaaaat gacctaaagc
    12361 aatatcattt gatccagtga ttttacgaat gatcggttgc gctactgccg gcatagcaat
    12421 ggcgaaacaa gctccgataa aaccaccgac caagatcaat acgatccctc gcagaccggc
    12481 aaaatagcca aagactgtcg tcatcgttgc catccataaa attgcttgac cagttaaaaa
    12541 aatgtacttc cattttgtga aacgagccaa aataatattg aatacaaaaa tggctaagaa
    12601 cgtcagtgca atatctctac ctaaacccaa atcattcatt gcttgaccat tgatagattc
    12661 aatagaaggg atgatccctt gcatatgaaa tccttcagta aagattttcc cgaaataagt
    12721 taaacttcca acgatgatgc tcgaaccggc actcaacact tgaaatccta ggagtgtctt
    12781 taacgtgcca gagatgactt gtcctgctga tttcttctgc agtagtagac ccaacatagc
    12841 aatcaatgca atggtgatcg atgcctgagt caaaatattt tcgatgataa aattgataac
    12901 gcccatgttt tgatgtgcac cccaaaagtt agactagaaa tctaattaaa aggggtgctt
    12961 tttttgcgga aatatacatt tgtttttaag aaaaaagtag tttcagacta tttaaataac
    13021 gaaggcggct acaaatatct tgcacataaa tatcaaataa atcgtacact ggttagacat
    13081 tgggtaagga tttataacta tcatggttgg gaaggcttgg ttggaggtgg caaaagctac
    13141 actacaaaat ttaaacttga tgttatagaa tatatggaaa caaatggtct ttctatccaa
    13201 gaaactgcta aaaaatttaa tatcggttca aatagaactc taagtaaatg gatagagcaa
    13261 tatgaagaag gcggtgcttc ttcacttgag agccaaaaaa ggggcagaaa aattagtatg
    13321 aattccaagc taaacattcc taaaaaactt aaagatgagt ctcttgaaga agaagttatt
    13381 cgtttaagag cagagaacgc atatttaaaa aagttagaaa ccttgattca agaacaagat
    13441 ttgagcaaga agaaatcaag gttaaaacaa tccttcagct caaaaaagaa tttaaattga
    13501 atctattact ttcaattgca caattagcga agtcaactta ttattactgg gtaaaaaaat
    13561 tagataagcc agataaatat agcaagataa agcaagaaat tacagcgatt gtaaaagaat
    13621 caagaaactc ttatggttat cgtagagtca ctttagcgtt aaagatgaaa ggatatacaa
    13681 tcaatcataa aacagttaga aaattaatgt cccaaatggg acttacctgt caaatccgaa
    13741 taaagcgata taaatcttat aaaggaacag taggaaaaat tgccaagaat gtgttaaaac
    13801 gaaatttttc agtagataca cccaataaaa aatgggtgac agacgtcact gaattcaaga
    13861 taaaaggaag aaaaatctat ctatctccta tacttgattt atttaacgga gaaataatta
    13921 gttatagtat atcaactagt ccaacatata aactgattga agaaatgctt caacaagcaa
    13981 ttaaaaaaaa gggaactgag ggttcactga ttctacattc agatcaagga tggcagtatc
    14041 agatgccaca atatcaaaaa aaattaaaag agaataacat tattcaaagc atgtctagaa
    14101 aaggaaattg cttagataat tctgtaatag aaaatttttt tggtgtgctt aaatcagagt
    14161 ttttttatcg agaaaaattc cgatcaattg agatatttca aagtaaatta aatgagtata
    14221 ttaggtggta taataacaaa aggataaaac taaagttaaa tggactatct ccagtagaat
    14281 atcggaaaca gtccattaaa tagtt
//
LOCUS       DAFGFZ010000074        14090 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000074 DAFGFZ010000000
VERSION     DAFGFZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.075"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            220..2385
                     /gene="acm"
                     /locus_tag="LR726_001925"
     CDS             220..2385
                     /gene="acm"
                     /locus_tag="LR726_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL6217011.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2657..3142)
                     /locus_tag="LR726_001926"
     CDS             complement(2657..3142)
                     /locus_tag="LR726_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL6217012.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3232..3577
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3622..4263
                     /locus_tag="LR726_001927"
     CDS             3622..4263
                     /locus_tag="LR726_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL6217013.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4282..4917
                     /locus_tag="LR726_001928"
     CDS             4282..4917
                     /locus_tag="LR726_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL6217014.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4935..5750
                     /locus_tag="LR726_001929"
     CDS             4935..5750
                     /locus_tag="LR726_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL6217015.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6069..7505
                     /locus_tag="LR726_001930"
     CDS             6069..7505
                     /locus_tag="LR726_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL6217016.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7694..10141
                     /locus_tag="LR726_001931"
     CDS             7694..10141
                     /locus_tag="LR726_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL6217017.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGESNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10371..11438
                     /gene="rlmN"
                     /locus_tag="LR726_001932"
     CDS             10371..11438
                     /gene="rlmN"
                     /locus_tag="LR726_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL6217018.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11631..12734)
                     /locus_tag="LR726_001933"
     CDS             complement(11631..12734)
                     /locus_tag="LR726_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL6217019.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13287..13835
                     /gene="aac(6')"
                     /locus_tag="LR726_001934"
     CDS             13287..13835
                     /gene="aac(6')"
                     /locus_tag="LR726_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL6217020.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4837 a         2322 c         2975 g         3956 t
ORIGIN      
        1 agtaaaataa aatgaatata tgcgaaaaaa taatttacat taggaaaaac cttgtaaaaa
       61 cagttttttg gcatatttat tttttataaa tatttgtaaa ataactatca attttttagt
      121 taaaagattg gaaatgagtg ggggaggaat tgaagaaagg atttttgaga gatgatatag
      181 taggttttat ttttagttaa aataggggag atagaaaaaa tgaaaaagtg tagaaatatt
      241 attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt tcatgtctat
      301 gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat
      361 attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc
      421 caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga
      481 ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga
      541 aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt
      601 tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga
      661 ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct
      721 ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag
      781 cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa
      841 atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa
      901 atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa
      961 gatttcccca gcgctacatt taatttttca gtaactgata attacattga aataactata
     1021 ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat catagaaaac
     1081 ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct
     1141 gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc
     1201 aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt
     1261 atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca aggacagtct
     1321 agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga
     1381 gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca
     1441 aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat
     1501 aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca
     1561 agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga accagttcct
     1621 gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa
     1681 cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga
     1741 aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag
     1801 aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac
     1861 ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac
     1921 aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga
     1981 gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg
     2041 attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca
     2101 ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga
     2161 aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga
     2221 agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt
     2281 cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt attgatagta
     2341 actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga atataatatt
     2401 ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc gaaaaaagaa
     2461 gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg gctaactatg
     2521 gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt tgattggcca
     2581 aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa
     2641 gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag taaacaagta
     2701 tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt ggatgaaagc
     2761 ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt ttctctttgt
     2821 cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg tcaggaagct
     2881 gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt tgttttaaat
     2941 cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt cttgatttca
     3001 ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg actgacgtaa
     3061 tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt tcataagctt
     3121 cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt gtatcataaa
     3181 aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa acaaatgaca
     3241 tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg gaaagaggca
     3301 gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt aggaaaatcc
     3361 ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg cttttccgtg
     3421 aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt cggatcaaag
     3481 gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa
     3541 taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga ggattttttt
     3601 attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca attattagta
     3661 ggagcactca caacattgaa attattttta tttaccttga ttggctcatt accattaggg
     3721 attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt gcttcaaaca
     3781 tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat tttttatggg
     3841 ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc ctttatctta
     3901 aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat tcctcaaggt
     3961 caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac gaaaatcgtc
     4021 atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat caacttggtc
     4081 aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc
     4141 gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat ctatcttaca
     4201 ttgactggta ttctaacaat cataatgaaa caagttgaag gaaaattaaa ttattaccgc
     4261 taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa
     4321 aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt agctatcgta
     4381 ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt agaaagttct
     4441 gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct taaaatgaaa
     4501 gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct gaatattttt
     4561 gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga atacaccaaa
     4621 aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat ctatccttat
     4681 cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc tatgaatcca
     4741 cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg ccagcaagtc
     4801 gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt cacccatgat
     4861 ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat tcattaagaa
     4921 aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc
     4981 tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact atcaacgaag
     5041 agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt agagataaag
     5101 acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa caatatggga
     5161 ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg aacaatggaa
     5221 cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag aaagtgcaat
     5281 ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa tcaggaatca
     5341 caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca tctggttatg
     5401 atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa gatgctgttt
     5461 tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc gatggtctgc
     5521 tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta gaagacttca
     5581 acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc aagtcagata
     5641 gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca ggtgaattcc
     5701 aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa caaatagggg
     5761 actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc tcctgccaac
     5821 tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg tgttcaaaag
     5881 ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt ttcacaaaat
     5941 ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata attttatttt
     6001 gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta taggaaagga
     6061 agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg tggggctctg
     6121 cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga gaatcagtct
     6181 gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt gatttggcag
     6241 tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa ggtctgaaag
     6301 cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga gaagtcaatc
     6361 aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac gatattgaac
     6421 ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa tataatggtt
     6481 gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta tttgaggcat
     6541 tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc acttcattag
     6601 gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta
     6661 accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag ttagaaatgc
     6721 ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac agtaatcctg
     6781 aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg atggatgtct
     6841 atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa ggatgggcac
     6901 caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat tttcttggga
     6961 tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt ggtgctggaa
     7021 aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct ggaatgtata
     7081 aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt gacccagaag
     7141 gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta ctgattacgg
     7201 aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat gacgactatc
     7261 gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg gatggagcag
     7321 aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg aacggttatc
     7381 aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga tctttggaac
     7441 gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat ggagagaacc
     7501 tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc ttttcttgat
     7561 aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca
     7621 gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa cttgatgaaa
     7681 tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa
     7741 aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg ggtttgcctt
     7801 gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat tgattgcaaa
     7861 cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat tagccgtatt
     7921 aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc gacgctggtt
     7981 tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt tatttggcaa
     8041 actccatact ggggaatcaa atatcatggg gacgacatgg gatttgttag ccagtgatat
     8101 aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa tcctttatca
     8161 ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc tattactggc
     8221 aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg gtatccaatc
     8281 aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag ctcgaaagca
     8341 agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc
     8401 tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag cggtgagcca
     8461 gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc cggagcagct
     8521 aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc tagagaaacc
     8581 agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg agttgccaga
     8641 ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc aagactatga
     8701 attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg atgagtataa
     8761 aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg gtgtcgatgc
     8821 caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt
     8881 aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg ctttagcggc
     8941 gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta ttgaagtacc
     9001 aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac caaaccaccc
     9061 agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac aaacggctga
     9121 tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga agtcagtagc
     9181 aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag taaaactgat
     9241 gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc atctgttgac
     9301 gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag tacaagaaat
     9361 ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg gttacaatga
     9421 atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac cattcatcgt
     9481 agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag ttgaagatgc
     9541 gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc tagctactca
     9601 acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt ccagaatggc
     9661 atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg gggcagaaaa
     9721 gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac cgatccgtgt
     9781 gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg ttacagatca
     9841 gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa cgacaggtgc
     9901 tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca
     9961 aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc gagcagcacg
    10021 tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg
    10081 gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata atgacatgta
    10141 aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag gcatgatgat
    10201 accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt cttgtctttt
    10261 tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat
    10321 gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac
    10381 agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga
    10441 aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca
    10501 cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca
    10561 atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg
    10621 aactccctga taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag
    10681 tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc
    10741 tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac
    10801 attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg
    10861 gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca
    10921 aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa
    10981 tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga
    11041 ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga
    11101 tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca
    11161 tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag
    11221 ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc
    11281 aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg
    11341 gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat
    11401 tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca
    11461 attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa
    11521 gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga
    11581 aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt
    11641 ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc
    11701 tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg
    11761 gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat
    11821 actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga
    11881 tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc
    11941 attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta
    12001 ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca
    12061 attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag
    12121 agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca
    12181 tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc
    12241 cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt
    12301 ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc
    12361 gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt
    12421 ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg
    12481 tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta
    12541 cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg
    12601 ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct
    12661 ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa
    12721 acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga
    12781 gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga
    12841 tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta
    12901 caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc
    12961 ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg
    13021 ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg
    13081 aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa
    13141 gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac
    13201 tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga
    13261 ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag
    13321 tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca
    13381 gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag
    13441 accaagatga gttagtagga tttattggtg caatccctca atacggtatc acaggttggg
    13501 aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag
    13561 tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg
    13621 atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata
    13681 aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt
    13741 ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg
    13801 caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa
    13861 tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt tgtgaaattc
    13921 caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg tgaaaaagtc
    13981 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa
    14041 tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa
//
LOCUS       DAFGFZ010000075        13807 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000075 DAFGFZ010000000
VERSION     DAFGFZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.076"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LR726_001935"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LR726_001935"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL6217021.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="LR726_001936"
     CDS             complement(2260..2511)
                     /locus_tag="LR726_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL6217022.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="LR726_001937"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="LR726_001937"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL6217023.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="LR726_001938"
     CDS             3461..3679
                     /locus_tag="LR726_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217024.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="LR726_001939"
     CDS             complement(3968..4480)
                     /locus_tag="LR726_001939"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL6217025.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LR726_001940"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LR726_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL6217026.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="LR726_001941"
     CDS             complement(6933..7376)
                     /locus_tag="LR726_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL6217027.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="LR726_001942"
     CDS             complement(7377..8165)
                     /locus_tag="LR726_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL6217028.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="LR726_001943"
     CDS             complement(8162..9103)
                     /locus_tag="LR726_001943"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL6217029.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="LR726_001944"
     CDS             complement(9280..9867)
                     /locus_tag="LR726_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL6217030.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LR726_001945"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LR726_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL6217031.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="LR726_001946"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="LR726_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL6217032.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="LR726_001947"
     CDS             complement(12784..13719)
                     /locus_tag="LR726_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6217033.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DAFGFZ010000076        13704 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000076 DAFGFZ010000000
VERSION     DAFGFZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.077"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            207..545
                     /locus_tag="LR726_001948"
     CDS             207..545
                     /locus_tag="LR726_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217034.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(750..989)
                     /locus_tag="LR726_001949"
     CDS             complement(750..989)
                     /locus_tag="LR726_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL6217035.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(965..1312)
                     /locus_tag="LR726_001950"
     CDS             complement(965..1312)
                     /locus_tag="LR726_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL6217036.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2044..2625)
                     /locus_tag="LR726_001951"
     CDS             complement(2044..2625)
                     /locus_tag="LR726_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6217037.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2808..3434)
                     /locus_tag="LR726_001952"
     CDS             complement(2808..3434)
                     /locus_tag="LR726_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL6217038.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3456..4787)
                     /locus_tag="LR726_001953"
     CDS             complement(3456..4787)
                     /locus_tag="LR726_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL6217039.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4942..5343)
                     /locus_tag="LR726_001954"
     CDS             complement(4942..5343)
                     /locus_tag="LR726_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL6217040.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5429..5635)
                     /locus_tag="LR726_001955"
     CDS             complement(5429..5635)
                     /locus_tag="LR726_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217041.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5730..7232)
                     /locus_tag="LR726_001956"
     CDS             complement(5730..7232)
                     /locus_tag="LR726_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL6217042.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7250..7705)
                     /gene="argR"
                     /locus_tag="LR726_001957"
     CDS             complement(7250..7705)
                     /gene="argR"
                     /locus_tag="LR726_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL6217043.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7803..8420)
                     /locus_tag="LR726_001958"
     CDS             complement(7803..8420)
                     /locus_tag="LR726_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL6217044.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8641..10014
                     /locus_tag="LR726_001959"
     CDS             8641..10014
                     /locus_tag="LR726_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217045.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10089..11201
                     /locus_tag="LR726_001960"
     CDS             10089..11201
                     /locus_tag="LR726_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL6217046.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11273..12910)
                     /locus_tag="LR726_001961"
     CDS             complement(11273..12910)
                     /locus_tag="LR726_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL6217047.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13078..13686)
                     /locus_tag="LR726_001962"
     CDS             complement(13078..13686)
                     /locus_tag="LR726_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL6217048.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3699 a         2880 c         2453 g         4672 t
ORIGIN      
        1 gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc cagttcattt
       61 tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca
      121 aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa
      181 tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct
      241 tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa
      301 ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta
      361 gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt
      421 tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag
      481 aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt
      541 cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa
      601 atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta
      661 tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt
      721 gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc
      781 tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct
      841 tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta
      901 gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata
      961 tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcattg
     1021 ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt ctttttttct tctactgtct
     1081 ctgcacgggc attaatcatc aattgtcctt ttttaaatcc ggtaaatccc cacttcatga
     1141 tttccggagt gaggatgttt tcttcctctt ttcttaacgt cacaacaaga tttgacggaa
     1201 aaacttctcc agctgctaat tctgctgcct gcttcggttg tttcgtctgg gcttctcgat
     1261 aataattctg aaggagctct gactctagat caaaaaaata acgaccacac atcctaatcc
     1321 ctcccaatct ttttttctat catacactgc aacaagaagt tcgcaccaac aaaacgcttt
     1381 caatttttgt agaactaaaa aagttgtgac aagtgacaaa ctctccagtg aatggtattc
     1441 tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgctttttg
     1501 aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac gacctctggg taaacggtgt
     1561 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa agctattttc
     1621 acaaaatttt tcttaatact cgaaactaag cgactttttc acaacctctt ccttaaacgg
     1681 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagcagctat
     1741 ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tctccactaa
     1801 acggtgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     1861 attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa cctctaatac
     1921 acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa aatttgagaa
     1981 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctctaa
     2041 ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa aattctgcct
     2101 aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga tttcagcaat
     2161 caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag aaagtgccag
     2221 gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca acagacggta
     2281 ttcttcatcc actgtactca actcaataat caaattacct acaggacagc catgataagt
     2341 gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg cttcttctgg
     2401 ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt ccatatgcgc
     2461 atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa attgaccttt
     2521 tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc cttttttata
     2581 aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata ttctcctttt
     2641 tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct taatcgttta
     2701 aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc tcatatagct
     2761 agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta tgatagttgc
     2821 tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt cgtcaacgct
     2881 gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac aacattgaat
     2941 ccactctcta ccaattcacg catatgggaa tcgctacaca agtttccgtt cattccacca
     3001 agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt gctttctggt
     3061 ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg tttcaattct
     3121 tcgataaaat cggctcctga accttcttcc actgattcat attgactttt tctagcaaac
     3181 atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt atggtcatgt
     3241 gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc ttgttttacc
     3301 atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt caaaccaaag
     3361 ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac tgctgtattt
     3421 ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc atttagagct
     3481 tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg aagttttcca
     3541 ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata gatacttgcc
     3601 cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt aggatcttct
     3661 aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta attgacgcct
     3721 tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt gataaataca
     3781 ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt caactgatcc
     3841 ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc tttgacgtct
     3901 ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc gattgctccg
     3961 cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact tggttttctt
     4021 ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc ttctgtaaag
     4081 gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc tagatggaat
     4141 tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc atcttcacgt
     4201 ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc attgaacatt
     4261 gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc cggtaattgt
     4321 tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa tcctttgatt
     4381 ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc aacaatcact
     4441 ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc tgtaccgtcc
     4501 atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg aagcatgccc
     4561 gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc tccattgaaa
     4621 atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata catacgtgtt
     4681 gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc taaagtctta
     4741 ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt tccctcgctt
     4801 cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta ttctccaaat
     4861 catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg tcctttcacg tttttagtct
     4921 ggttcgattt cctttattct tctaaacaat cgttacttct acatcgatat ttcctctagt
     4981 tgctttggag taagggcaga attcatgcgc atcttctgca attttttggg cttcttcttc
     5041 gctgatgcca tcgatatgca cttctaatac tacaccgatc tgatagccgc catcatctaa
     5101 attataaaga gagacacgtg cttttactgt ggactctgct gtcattcctg cttctttgat
     5161 catatgttgt accgccccat tgaaacaaga agcataacct gctgcaaata attgttctgg
     5221 atttgttcct tctgcatgga ttcctggagg agtgattttc atatgcatcg agccatttgg
     5281 tgcctcgact tctccattgc gcccgccatg gttgatcatt gctgtttcgt atgctttttt
     5341 cattgtactg ctcctttcga ttgaccattt gctacttggc tataattata gtgtatgaca
     5401 agtaaggcgc tttctacaaa aattctgctt attcgaatac cagaatgccg tcttctacta
     5461 tttttccaaa aaacaaaaaa atactactcg tgaacaacag cgtcatattt gttccaaaat
     5521 aaatagcaat cttttctaca taatctttta tatgattgat tttaagatac atatatacca
     5581 gaaagttgac cccataaacc aatactgtta aaaaaataac catattcgtt accatttttt
     5641 cctccttttg ctggtctccc taccagcact cgatttaacc ataccacttt ttttgccgtc
     5701 caagcaacta gcctttcaaa aagactggtt tattctgctg ttaatgattc aagatcagaa
     5761 aaatgtccga ataattttgc taattttact aacgccaact tgttctttcc gaattcatca
     5821 ttttctatct gtaatactag taacggctca tgtaagctta aacggatcaa aaaccagccg
     5881 tttccgtatg gctcttctag atcatatcga ataccttctt cattttccgg gtcgaagttc
     5941 atcccttttt cttcttccaa cagtcgcttc atttcttcta atatcttgtt tccttgttcg
     6001 cgtggttcat cgctgaacaa ttttaatcgg atttcttgta cttctacggg ctgtttcaac
     6061 tctttgattt gttcatctaa tgtgatacct tttcttttta attctggcaa aagcatcagg
     6121 atctttgcga tgacatatgc accatcatcc agaaaatagt tttccttgaa tgcagcgtgt
     6181 ccgcttgttt cgatggctag ctgtgtatct ttcccaagac ggttaagttc taatgcttta
     6241 ttgattacat ttcgatagcc ggaaatataa cgaacttgga tgcctccttt ttcttcaatg
     6301 aatctttgta gatgagaaga agtaggggaa ttcgtcacga ttcttgttcc tggatgctct
     6361 tttaatacga ttgtacttaa tactgcgatc aaattatttc gattgatcac tgttccagaa
     6421 gcactgacaa ctgcagaacg atccacatct gtatcgaaaa tgatcccaag atctgcttca
     6481 tttgctaaaa cagcctgctt gatactttcc atcgcttctt tattatcagg atttgggata
     6541 tgattaggaa atcggccatc tggttccaaa aattgggaac ctgttgaatc cgcacctaag
     6601 ctattcaata ctttttcagc aaagaatcca ccagcaccat ttccggcatc aacaatgatt
     6661 ttccaaccat ttagtggttt gctttctagt gttccaatcc cctttttaat ttttgtgact
     6721 aaatcttgcg cataaggtgt caaaaggtct gctttttcta cgcttccagt ttgttcgctt
     6781 tttatttttt gctgctgtga taggataaaa gcaatatctt ctttttctgc tcctccttct
     6841 gaggtaaaga attttattcc attataataa tagggaagat ggctagcagt cagcataacc
     6901 gcagcatcgc acgagaactg ttcaaattga gtggacataa acatagcagg cgtagtagct
     6961 aattcgaaat cgatgacatg tatcccttgt tctttcagac tttggatcaa cgcctctttc
     7021 agtgctggtc cgcttatcct actgtcatga ccaatcccaa tcgtccattt tttatttgga
     7081 tcggttgatt tcttcttttt ctttatccag tttgctagtc cccagccgat cttttttacc
     7141 atgtctgtcg tcaagtttac ttcatatcct tcaactggta atgcgattcc tcgaatatcc
     7201 gatccatttt gcagcgcttt catatctttc atataaaagt cctcctcatt tattccctca
     7261 ttttttccag cttttctgca aatcgctgtg cttcttcttc tgagcggcaa ataataatga
     7321 atgtatctac accggccatc gttccgacaa cttcagggta agccacttca tctagccaat
     7381 tcgttaccac atcggcacca tttcgttcag tcaatacgat aaccatgaac tgaacagact
     7441 ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg ttcttcattt aaagaaacag
     7501 atggctggtt aaataatgta tagcgtacta ttttgttttc attttgactt tttactaact
     7561 tcatttctcg aatgtctcta gaaatcgttg cttgagtcac atcggcgcct gcttcatgca
     7621 gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc tgtaatgatc tggcggatca
     7681 gtgcttgtcg ctgtttcttt ttcatattag acacctctca atgttgatta tagcaaaaga
     7741 taagtaatat ttcttcatat tttggtattt tttactttta ttttttgttt tcattcaggt
     7801 tgctaacctt tttctatttt aacacgcaaa atcgtcttca ttttttcagg tgctgatttt
     7861 cttggatcgc tgatgtaaat ctctttgtgt tctttagaag ctctttcata gccttctttt
     7921 gcgatgaatg cttctaattt ctcaaacgtt gctggttctt cggcataggg cccgacgtgt
     7981 aagatttggg cacaaagacc ttcttccatc tttttgaata ctacttgttc tttcaagtct
     8041 tccggaagtt tgctagaacg ctgaatggct tcttgtgcta tcgcttctgt aacaaagtca
     8101 ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt tcatcactgg ttcatgcaaa
     8161 tacttttctt gcagtcccca ttgtccttcc agtggataga cagtatacat ctgataatca
     8221 ggaatcagcc aatttttttt aggcgacatg cgtattgcat agctgactgc atatagacag
     8281 gctactcttc gtttaaagtc ttcctgattc gggtcgccga tccctctgat gctaaagaat
     8341 gattttttag gaacctctag tatttgcggg ctttttttca tactatagct cttgtcttta
     8401 cgccattcaa ttttttccat ttttcttcct ccgcgaaatt ccatttagtt atttattatc
     8461 atacctcttt ttgagtgact tggctaatcg tgttgacact atttttcagt ttgtgctaga
     8521 ataagtgaag taaataaatc gtacagataa cacgtttcgg cgtgtcgaga gagaggcggt
     8581 ttctattttt ataatagaaa tcactctctt tttttacgaa attttttaga ggtgatgtaa
     8641 atgacaaaac aaaaacagct gcttttagta cctgcagtaa ttgcaacagg gatgatgtct
     8701 tttgcgggcg tattgatcga gacggccatg aatgtaactt tcccaactct tatcagacag
     8761 tttgatttaa cgactgcaca agtccaatgg gtaacaacga tttatctttt gatgatttcg
     8821 atcattgttc cgctgtctac ttatttgaat cgcaatttta gtctcaaaaa actcttttta
     8881 gcttcaaatc tgcttttctt ggcaggtata atgatcgatt tcttctctcc taccttcatg
     8941 gtcctattgt ttggtcgatt gctccaaggt gcagcgacag gtatcgctct tccgttgatg
     9001 ttccatatta tcttgacatt tgcccccctc aatcgacgag gcgcgatgat gggtgtcggt
     9061 actttgacaa cggccattgc tccagcaatt ggccccactt atggaggcat tatgacttca
     9121 cattttacat ggaatcatat cttcctgttt cttgtgcccg ttttgtttct ttctttgatt
     9181 atcggctggt ttgctattcc cgagatggca gtacaaaaaa gtggttattt ggattggata
     9241 agcgttttag gaatttgtct tttgtttagt ggaagtctaa catttttgag tacgttggga
     9301 acagtttcca gctggattgc tttaatcatc ggcggtattg gctggttcct tttttactat
     9361 cgaagtaaaa aagtggagca tccactgatc cgactgtcta ttctttcaaa taaaacattc
     9421 cgcttgtttt tgattggctt cttagtttat cagtttttat tacttggtgt atcttttgtc
     9481 ttgccgaatt tccttcagat cgttcaagga agtagtcctt ttgtcgctgg attagcgatg
     9541 cttcccggcg cagcggttgg tgctttgctt tctccttttt ccgggaaaat gctggatcaa
     9601 tatggtccga agaagccaat ttttgctggg ctgattcttt ctttgattgg ttggctagca
     9661 ttattcctac tcataggaca cgcttcagtg ggtgtattga ttgcttgtca tgtgttttat
     9721 atgatcggta tcggattatc ttacagcaat atgatgacca ccggaatgaa tgcactgaat
     9781 gaagaactac aaggagacgg gaacgcaatg ttcaatacct tgcagcagtt ttctggcgct
     9841 gtggctactt cattagtagc ggtcattatc aattacgtcc agcatcatac ttcccataac
     9901 tatgaagtgt ctactacatt aggatcaaaa gcagcattag gcgtcttgtt gctgttgctt
     9961 ctcgtaagct ttgctcgatt cgcctattat ctgttctttg ctaaaaaagc gtagattcca
    10021 tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa atggttgttt gtaaaaggag
    10081 gcttttttat ggaaaaaatt gccattatcg gtggcgggat cattggtatg accctagcca
    10141 gttacttaga tacatcaaaa ttcaatgttg ctttatttga tgaagggaca ggacaggcaa
    10201 ctaaagctag cgctgggatt atctcccctt ggttgtcgaa acgcagaaac aaacaatggt
    10261 atcgtctggc taaagacggc gctgcttttt tccctaaact tataaaagat ttccaacttg
    10321 atcaaacgat ttacgcacaa tcagggacac ttttgcttcg acctgaaaaa gatttagctg
    10381 aattggctgt tcttgccgag aaacgaaaag aagaagcgcc agaaattgga aacatcgaat
    10441 ggctttcacc tgaacagaca tctcaagcac ttccgttatt gaaaccagca gtttcactgc
    10501 gtatcagtgg tggtggtcgt ctggatggaa aagcatattt agaacatctt ggtcagcgtt
    10561 tagcacaaaa acaaatggag cagatccagg caaaagtcac tttgcataag aagagcgatt
    10621 cttggctcat caagggaccg tttggagaat acacagctga tcgtgtaatt ttgacacctg
    10681 gcccggcact taagccttta ttacaagaat taggctatgc tgcagatata cgtccgcaaa
    10741 aagggcagct cttagcattt cagacatctt ataagatgag tcaagactgg cctgttgcta
    10801 tgcttgacgg agaagctgat ttcattcctt tcatggatgg aaagatcctt ttaggtgcta
    10861 cccatgaaaa cgatcaaggc tgggatttgg ctcctactag cgaagcttat aagggattga
    10921 ctaagagtac agaacagttc ttgactgatc cagatcagat aaaaaactgg ccatacgagt
    10981 tccgtgtagg tacccgtgct tatacgtcag atttttcccc atttttcggc gtactgccag
    11041 atgatccaag cttactggct gctagcggtc taggctcttc tggcttgacc acaggtcctt
    11101 atatcggcta tctattagcc aacttcttaa atactggtca ttggcctacg gaagattatc
    11161 aaaaggacat ctccacctat attacgaaaa atccatcata gaaaaacagc tttttcaagt
    11221 aaggatcccg agcgatattg cgcatggatt acttcttgaa aaagctgtta ttttagttca
    11281 atttctgaat caaattctcc aaactttctt ttgcttcatc tggcaagaga tcttttccta
    11341 tctgagcttc aatgaatcgt aaacgaatat caagtcgttg tttcaccaga tttttatagg
    11401 acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa atagtggatg tcttcaaatg
    11461 gaccaaacga cttgaattct gccgtttgtt gcacgattct ttcaacgatt gacaatgtcg
    11521 tttcaggttt gattttttta tcatcgaaat atccagtatt caggacatag caggctgttt
    11581 gctgtttcaa aaacaactct cgaaaagctt gatagtcttc cctaagagga tagacacgga
    11641 aaggattagc aaatggttca atgactagct catctagctg tctttttgta gtgatatttt
    11701 ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga cgctgctaaa actgggtcat
    11761 tgatcttgat aattggcgga agcgaatcat ctttcatgat ccaaaaaaca gcatcgatct
    11821 tttcgctaag atgatcgact cggtcgggtg tcgcataacg tgatttgact gtccgcccat
    11881 ttccgtttcg tatatctcct gttactagga ctttttctcc tttacttgta catgtcacgc
    11941 ccacattctg acaagtaagg aaagaagaaa tggccggatc atcagacgga tagtcttgtg
    12001 ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc attttttcga gaaataacga
    12061 aagcatcgtc atgcaagatc gaaacagagt gaccttccgt atgttttgct aaggtaatcg
    12121 ttgattttcc agaaccggac aacccaaata cagccatcgt ataggtctgg tcattactag
    12181 catattgttt catcccaccg tggcaggcaa taaagccatt gcgatttccc aatgcccaag
    12241 caagcgtcaa agtcgctttt tttaattctc caaagtaacg taatcctagc acagccgctg
    12301 tattatggag agcatcaaac aaaatgagtc catttggaaa tgtttcatgt gtccattcgg
    12361 gatcacaaaa gatataaaga tctccctcat caaaggaaac agacttttga taagtctcta
    12421 agatcttcga attcgctggc tgaaaattca gcaaataaga atacaaattg ttctcaaatc
    12481 cttctggaat cagcaaatga ctttgcacag aaaattcttc agataggcca acgattactt
    12541 ttgtatggta aaactggcgc ttgcgacttt gatacacagc ttctcttaaa attttctcgt
    12601 atttgctttg ttccttgtct aagtcctcaa agatccgccg agcacttgct gttcttccca
    12661 caatgcttcc atcattgaat actagtgtcc taacatcagc tggtaaatct agttcttcag
    12721 catgtgagat tgttaaatct gtgatgatcg ttcctggact cccttttgct agttgataag
    12781 ctgtcttcct atcaaaaact tcatgaacat tattaccgta aaatgtcgtt tctacaatcg
    12841 atcgaatact ggataacagt ggatttccac ttgtgatttc gtttaattcg agactctctt
    12901 ttgtactcat ggctcctcct cattccctaa acaaaaaaac gttttcttta ttatacatta
    12961 ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa cgagttattt ttcacacaaa
    13021 aaaaccgaga acttgaataa acaagtttct cggtttcatc attttccttt tatcggctta
    13081 gtaccagccg tttgctaacc agaatgtttt agcagcatcc catgaaccat aacgagatgt
    13141 tacatattgt tctgctacac gttcttgatt ggcagctgaa tagtcgccgt ttaagtatga
    13201 agcatctaat tgataacggc cgatgtaacg tccgttagta gctgtgtatg aaccgcctga
    13261 ttctttttgt gcgatccatt cttttgcaga gcttgaagtt ggcgctgttg cagcagctgg
    13321 ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg tatgtatgtt cttgcgttgc
    13381 tgtttcagtt gtagatgctg tattttctgt tgttttgttt tctgttgttg ctgaagaatc
    13441 atttgaagtt gggattgtta attgttcacc aacataaatg cggttgatgt ttgcgatcga
    13501 atttttttca gcaattgcat cgatcattga gttgtctcct gcaaattttt gtgagatctt
    13561 agataatgaa tcacctgatt ttactgtata aacttcatca gcgtgagcat ttgtagttcc
    13621 tacaaacatt gcagtagcac cagcagcaaa agttgtacct aaaacaattt tcttaaatga
    13681 attcatatta aactcctttt tttc
//
LOCUS       DAFGFZ010000077        13516 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000077 DAFGFZ010000000
VERSION     DAFGFZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.079"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..702)
                     /gene="msr(C)"
                     /locus_tag="LR726_001963"
     CDS             complement(<1..702)
                     /gene="msr(C)"
                     /locus_tag="LR726_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL6217049.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKK"
     gene            complement(1056..1685)
                     /locus_tag="LR726_001964"
     CDS             complement(1056..1685)
                     /locus_tag="LR726_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL6217050.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(1669..2133)
                     /locus_tag="LR726_001965"
     CDS             complement(1669..2133)
                     /locus_tag="LR726_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL6217051.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(2620..3888)
                     /locus_tag="LR726_001966"
     CDS             complement(2620..3888)
                     /locus_tag="LR726_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL6217052.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(4119..4856)
                     /locus_tag="LR726_001967"
     CDS             complement(4119..4856)
                     /locus_tag="LR726_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL6217053.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(4923..5492)
                     /locus_tag="LR726_001968"
     CDS             complement(4923..5492)
                     /locus_tag="LR726_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL6217054.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5620..6492
                     /locus_tag="LR726_001969"
     CDS             5620..6492
                     /locus_tag="LR726_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL6217055.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            6834..7706
                     /locus_tag="LR726_001970"
     CDS             6834..7706
                     /locus_tag="LR726_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL6217056.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            7703..9661
                     /locus_tag="LR726_001971"
     CDS             7703..9661
                     /locus_tag="LR726_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6217057.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9993..10844)
                     /locus_tag="LR726_001972"
     CDS             complement(9993..10844)
                     /locus_tag="LR726_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL6217058.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            10967..11434
                     /locus_tag="LR726_001973"
     CDS             10967..11434
                     /locus_tag="LR726_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL6217059.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(11697..12179)
                     /locus_tag="LR726_001974"
     CDS             complement(11697..12179)
                     /locus_tag="LR726_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL6217060.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(12279..13142)
                     /locus_tag="LR726_001975"
     CDS             complement(12279..13142)
                     /locus_tag="LR726_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL6217061.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         3937 a         2763 c         2427 g         4389 t
ORIGIN      
        1 tttcttttta ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga
       61 tttttccaat cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt
      121 ctccaattcc ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc
      181 gtcttttact tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac
      241 aaaaaccagc gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct
      301 atctagatgg gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg
      361 tatcaactcg atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag
      421 tcttaactgg aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt
      481 taattgaggc aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag
      541 ttcaccggct attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc
      601 tatacgatct tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa
      661 gctgacttgt agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt
      721 tcatgtaaaa caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa
      781 ctgcatcaca tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc
      841 atagctttca cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg
      901 ttgtttatac atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt
      961 catcgatgca gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa
     1021 aaagcaatag ctactgaata gtaactgaaa accttttatt cgaacatagt gatcttttct
     1081 ggtgctaaac gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata
     1141 actggaacat aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat
     1201 ccaccaattg gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc
     1261 gctaagctag aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa
     1321 taaggaatga tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg
     1381 taagcttggt tgtagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg
     1441 accattgcag aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt
     1501 tcttttgctt cctcgctttc agcgaccaca aagcgccatg gtttcatatt gacagaagaa
     1561 ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt
     1621 tcatcatata cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt
     1681 tccattatta ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt
     1741 atctaaaagt gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga
     1801 acctgtatct ttatggtcca tttcacaatg cttgattttc ttctgggctt tttctgttag
     1861 cgctacatac acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc
     1921 ttccaatacc ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa
     1981 actgctttgt agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag
     2041 acttataccg atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg
     2101 atataaaagg tgactaatct cttctaaaga cataatttcc tcctttctaa taattgactc
     2161 gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag aatcagctgt
     2221 gtaataaaaa aaacgctctc aatagaaaag tatagagaaa agaaattttt tgtagaatga
     2281 acggaaaaaa tgataaagaa agcacaacgc taaaaccaag attattgatt cttatcaaac
     2341 tatagaagat agctcttctg cagtagataa ttttacaccg ccaaaaattt ccgaaatcgc
     2401 cttttgcaag aatcccgtcg aatattagaa attggatcag tatgcttaaa cagtaataaa
     2461 aaagttatga caagatgttg tcacaacctt ttttgagtaa acggtgttca aaaacttgca
     2521 tctgcggtaa taagttcaac caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc
     2581 ttactacttg atgcagggca gctttttcac tacctctttt taataaccat aaaatcctat
     2641 tccttcatat ctccagccca tcggtgttag ccaatctctt tccttagcac tcaaggtata
     2701 gtgatgggta ccagtttttt catttggatt aaatagtcga taaattggct tggaaccacc
     2761 agaataaaaa cttagacctt cataacgcca accgattttt gttaaagagt tgatctcaga
     2821 ttgtgccatt gtgtaatgat ggtctccact gtaaggattg tataaacggt agacaggttg
     2881 tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc cagcctacag ttggcagata
     2941 atttttttct ccggcattca atgtatataa atgttctcct gtatgacggt tatacagacg
     3001 atacatcgga gaagttgaag ttggtgctgt atcatactgt gtcagattat aggtttcgat
     3061 tacattgttc aatgtagaac cataattagg cgcagtggca taacgacctg ttagccaagc
     3121 cgtagcatct ttgtaagagt tcgtattgct tttccatgca ccggaatagt agtaaacgcc
     3181 tggctgaaaa gaagtcgtct tcaataccgt tacgttatct tgcaatgatt ctttgtacga
     3241 aggatatttt ctaaaagcgg cattcaccgt aatccattgc ccattcacat attccgaagt
     3301 aggcatcgta acagattggc cattatagct tcctttaatc ccaaatagat tgtaattagg
     3361 ggcagaagcc aaagtactgt tcccccagcc actttcaacg atggcttgag caatcatgac
     3421 tgatgcatat aagtcattgg cacttgccaa actttgtgca gttgaggcaa tactatcaat
     3481 gaacgcttgt tgagaattag cagatgagcg agcataaaaa gcatagccag tatagttttc
     3541 gaattcagac aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt
     3601 cgtgatttct tctgcattct ctggattgtt ctgagcttct gctgatgtac tgtcttcggt
     3661 ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt gaaattgtat tttctgagac
     3721 agtagctgac tgcaaagtac tttctgtatc gttttcaatt cctgtttctg aagaactcga
     3781 tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc actgttgtgt tggcaactgc
     3841 tagatttcct aaaagaataa cgctaaataa taaattcttt gctttcatct tttttctcct
     3901 ttgaattatg aaacttttcc taaataacag tgtaactaaa aaagtaaaag caagtcaatg
     3961 tccatattta aataacattt gtttcttttt aataaatttg ctttttctgc ataaaaagac
     4021 gtaaatgaag aatattcttc tccattcacg ccttaaattg atggtcgttt gaataactat
     4081 ttaacaagta attctctctg gatattatcc gttatggatt atacttcctg tgtcgtcgga
     4141 cggataatca tgttgctgac agacatccgg tctggcgttt ctatcgcaaa tgctacagca
     4201 gaagctatat cttcagatgt taatccccat tctttttgcg cttcatgaag ttcaagagca
     4261 gcctttttgt cgctgatggt ttgatataat tcagtttgaa cagctcctgg agagatgatc
     4321 gtagatttga tgttattttg ccgttgctct tgacgtaatc cttccatgat tgcacggaca
     4381 gcaaattttg ttccgcaata aacggcggaa tcaggataaa caacatgtcc cgccacagaa
     4441 tcagttgaaa tgatatgccc agatttttgt ttttccatga ttggaagtac ggcggaaatt
     4501 ccatttaaca cacccatgat gttgatatca agcatatttt gccattcatc acggtgacct
     4561 tcaactaatg gagcggttgg catgattcct gcattattgt acaatacatc gattcgaccg
     4621 aacttttcca tcgctaaatc gataactgct tggacttgtg caaaatctct tacatctgct
     4681 gtttgaatat atagctcagc ttctggtaaa gattctttta ttgcttttag gcgatcttct
     4741 cggcgtgcag ctataactag tttagcccct ttttctgcta atagcttcgt tgttgcttca
     4801 ccgattccgc ttgaagcgcc catgatgaca atcacttttt ctgataaatc attcattcta
     4861 ttctcctcct gttttgaaaa atctcttgct tatactcact atctcttatt tcatcttact
     4921 gttcaaataa tccgttcgac aggtttgacg attttggcag ggattcctgc aacgatggta
     4981 ttgtctggga catctttcgt aacagttgca ttcgctgcaa caatggcatt ctcgccaatc
     5041 gttacgcctg gcagaatagt tgctcctgct ccgatccaag catttttctt gatgtggatt
     5101 ggctttactg acaagcctct tcgttctttt ggatcggtta gatggttcac cgtgatgagg
     5161 ttgacacgag gaccaatcaa tactgaatct tctaaacaga tgccacctaa gtcaacgaac
     5221 atcacttgac gattgataaa gacatttttc cctatactaa tatgtttgcc gaaatctgta
     5281 taaaaaggca gcagaacagt caccgtatca tctactttgt cactaatgat attttctagc
     5341 cgtttgcgga tatctgctgg ttcatggtac ccagtattca gcgctgctaa aggctcttcg
     5401 ttttccgctt ggatctgatg gatcacttca aagagcggat ccgttactcg aatctcttta
     5461 ttgacgattt gtttttcgat gaggcttgtc attttgctcc ctccttttat ccttacttta
     5521 taacggacgg cttatgatgt ctaatactta taaaacatac ttaactattc cttttaacta
     5581 tacttagtgg tatgcttttt taaaaacagg agggaaaaaa tggatattcg tatactgacg
     5641 tattttttag cagtagctag agaaaaaaat atcagccgag cagcaaaatc tctgcatatc
     5701 acgcaaccta ctctctcaaa acaactgaaa gatttagaag aagaacttgg tgtgacactt
     5761 ttcactcgag gctcccgaga gatacagttg acggattctg gtcgatatct ttattcaaaa
     5821 ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc
     5881 atctctggag aaatctacat tggcggaggc gaaactaaag caatctcttt catttctgat
     5941 agccttcatt cgttgatcaa ggcgcatcct gatatccagt ttcatttata tagcgggaat
     6001 gcagatgata tcatagacaa actggatagc ggattacttg attttggact cgtaatcgaa
     6061 ccgacaaaca aacaaaaata ccagtcattt cgactgccag cccaagatcg ttggggactt
     6121 ttgatacatc ggaatcatcc tttagcaaag caaacagtca tccatccgca ggaactgaaa
     6181 actgttcctt tgatcatttc ccggcaaacg tcagtggaca gtcaactagc tgaatggcta
     6241 ggtttcagca tagatcagtt atcgatcgtc ggaacatata acttattgta caatgcttct
     6301 ttgatggtcg ataacgggac ttttggtgca ttagctctag atgggatcat caatacttat
     6361 gggacggaaa aagtattcat cccactcgat ccgccattac aagcttcact gaatctcatt
     6421 tggaaaaaag accagcctct ttctcctgct gctcgtaaat ttttagatgt cctaacggag
     6481 aaatacagtt aagtagagtt tttttccttc gctttcagct ttttaacaac gaataagtta
     6541 agcaaaagat ccaatgatta aataacataa gtttcttaat ttgttccttc cttttatttc
     6601 tgataattcc agtaaattac tataattata aaaaaagaat tgacaaaaca taactgtcta
     6661 tataaaattg attacgttaa caataattcg tttcatgtga ctagatcata ctaggcatga
     6721 aaaatctctg aggaacatgg tttccctagg aggtttttcg tgtctatttt tgtttggccg
     6781 caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa
     6841 tcaaaaagat ctttaatcaa aatgcagtat tggtagatga cggtggacag gaaaaagtag
     6901 ctattggaaa agggattgga tttgacagaa aaagaaatga tttgattttt gatcgtgatg
     6961 tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc
     7021 agattgatga acgcttttta tttgcagcag aaacgatcat tgatcatgcg gaaactgttt
     7081 tgatggaaaa gttgaatgaa catgtattga tcgctttgac cgaccacatt gctttttctg
     7141 cggagaacat taataacggt atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc
     7201 tttacagtga tgaattcagt attgctcaat gggcagtaga gtatctgaca aaagagcttg
     7261 ggattccata tacttatgac gaagcaggat atatcgcaat ccatattcac agcggacgaa
     7321 gcggtcggaa caataaccat cgcagtatcc gtgaagtgac gattgtatct gacatcatcc
     7381 atttagtgga atcagaattg gaaacagata tccattcaga accattttca ctgaattatt
     7441 caagactggt gaatcattta cgactgcttc tacaacgaac ccatgcgcaa caatatgctg
     7501 ttttagacac ggaaatcgta gaaatggtca aacgaaaata cccagaaagc tataagattt
     7561 ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg aaagaagaac
     7621 tgggttactt ggccatccat atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta
     7681 acaatcatga ggaggaaaaa aattgaaagc atatctacaa cgaatgggac gttcccttca
     7741 acttccagtt gcggtcttac ctgctgctgc tttactagta gggatcggaa actggtgggc
     7801 atcttatagt aacgacattg tcgctcattt tttacaagct ggcggtaacg cagttttagg
     7861 tcaattacct ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg acaaatcagg
     7921 tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt taaaaactga
     7981 ttcagtagct actttattga atatcaaacc agaatcagtt gatccatctt ttgctcagat
     8041 cagtaacgta tttattggta ttattgccgg attgattgcg gcagctcttt ataatcgttt
     8101 ccacgaagca aaattaccga tggctttatc tttctttagc ggcaagcgtc tagtgccaat
     8161 cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt
     8221 ttactctgct ttagtttctt ttgctaaatt cattttgagt ttaggctggg gtggtgcagg
     8281 tctttatggc ttcttcaacc gtttactgat ccctacagga ttgcatcaag caatgaactc
     8341 cgtgttctgg tttgacgtag caggaatcaa tgatattggt aaatttttgg cttctgaagg
     8401 aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc ccaatcatga tgttcgggct
     8461 tccagcagga gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc
     8521 ttccttgatg ttagcagcag cttttgcttc attctttact ggtgtaacag aaccacttga
     8581 attctcattt atgttcgttg cttggccatt atatgtcttg catgcaattt tcactgggat
     8641 ttctttagca gtgagtgcat tcttccattg gacagcagga tttgccttta gtgccggatt
     8701 tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat cagccattga tgttgatcgt
     8761 ccaaggatta gtaacagcag tgatttacta ctttggcttt aactttgcta tcaaaaaatt
     8821 ccatctgatg actcctggcc gcgaggaagc agatctttca gatgacgata ctgcaacgac
     8881 taattcagac aataaatatg cggcacaagc aagcaaaatc tacgctgcat taggcggagc
     8941 cgacaatgta acttcgatcg ataactgtac tacacgcctt cgtttgcaag tcaaagatac
     9001 aggcctgatc gatcaaaata aaatcaaagc cactggcgta cctggtatga agatcatcga
     9061 cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag atgagatggc
     9121 aaaactacat ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg ttaaaacaga
     9181 aacatttgcc ggtggaacga tcgaacaaga tatttacgct atcgcaaatg ggaaactgat
     9241 tccaatcact gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg acggctatgc
     9301 tgtattacca gaaaatggag aaatcttctc tccaatcgct ggtacgatca caaacatttt
     9361 cccaacgaaa catgctgtcg gaatccaaac agatgctggt atcgaagtct tgcttcatat
     9421 gggcatcaac actgttgact taaaaggcga accattcact ttatatgtaa aagaaggtca
     9481 aaaagtcgca agaggtcaat tgattgcttt agttgatctt gccgctattc aatctgcagg
     9541 caaaaacacg gatatggtcg ttgtattcac aaatggcgac aaagtccaaa gcttagaaat
     9601 cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca acaaagcctg
     9661 agccatcgtc ttccccagac ttttttatcg ataaacaaac aggagctgag acaaaaatct
     9721 tgtctcagct cctttttccg aataaaaacg gcgttctcct gccaactccg cggtaataag
     9781 tccagccaaa cgtaacatat gaagagctat tttttatttg actcttctta ttacttgatg
     9841 cagaacagct tttttacaac ctctgataaa cggtgttcaa aaaccagttt atcggtatga
     9901 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt catactcaaa
     9961 actaatcggc tttttcacaa cctctttata cattattcct gatccaatat ttcttttaat
    10021 gctgcttcaa gagaagttaa tggttttccc aatacttttt ctaaatcatc agaggaaata
    10081 gctaactgat cctttttaat atcatgttgg atcgctaaaa acatgtcagc tgcttcttga
    10141 gggaatccgg cttcttttag attttcgaca aacgctgcat cacttgcttc aataatttct
    10201 aactttttac ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc agttagattg
    10261 ccggataatt ctaagatttc tggagagtca ctttctacta gtacttttgc tgcagcttct
    10321 gcatattcac gtttcaatgc ccaacctgtt tttccatttc ctgcagcata aacgaattga
    10381 ccagttttta aagcctgccc aatgataggt aattcattct ctacatacca attattccgt
    10441 aaaaatgtat gggaaatacc tgaatcctta atcatttttt cagtgaattg gtgatctggc
    10501 gctaatacac tgatcgagtg atcggcatct gcaaaacttg tataagcgat ataagatacc
    10561 ccagctgctt gtgctgcctt cactacattc ttatgttcgg cttgacgatt tcctggaacg
    10621 ccagagacaa aaagcaatcg atctattcct ttcaacgctt ctttcattga gtctaaatca
    10681 tcataatcac caatacgtat attaaatcca gcttctttca gtgcttctcc tttttcttca
    10741 ctacgtacta gtacatagat ttcattttgt ggaaccatct tttttaagaa ttccagcgca
    10801 taaccaccaa aacctccagt tgctcctgtt actaaataat gcatatttgc cactccctat
    10861 tctttttatt tgttaagatt tacagctgta catttataaa ttacaccttt atcataaaga
    10921 gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga caactgatga agtattctct
    10981 gcaatttagt gatgcaatcc atgtacttgc ctatattgaa atgtttcaag atacccgatc
    11041 actttctagt gagatgattg ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat
    11101 catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac cgcagccttc
    11161 ccttgccaaa agtccagatc agatttcttt actagatgtc tatcgcagca tcgaaggtaa
    11221 tacacagctt atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat
    11281 ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag aagaaatggc
    11341 taagatcaca cttgatagtc tgatacataa aatagccgtt ttggaaaaag aaaaaagacc
    11401 agaaaacaca ggcctcgtag agagattttt ataattctct ctactgtgcc tgcatttttc
    11461 tgatcggaat cgtctacata aattctttta ttctccgggg gacaaaagcc ttgtcatgcc
    11521 cctttttcct ctctaatgct caggtcaaag cgctgaagtc atgacgtctt gataaacggt
    11581 gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga aagctatttc
    11641 ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt cacgacctct atattatcat
    11701 ggtttattga ctaccggtat ccagatttct cccatatatt tcccgttggt aactcccatt
    11761 tcgactacag catttggacc cccaacatag gcgtaatctt ctacttctct taaaacatgc
    11821 ccaaaggccg tattcgtcaa atcatttctt aattcttcag gcgattctgc taaagcgatt
    11881 acaacaagat acgttccttt tgggaatggg atgattcggg tagtgtttgg aacatcctga
    11941 ctcgtttgaa caccgacata gtacatcatt ttcccgttat agggttcgtt gactgtaaag
    12001 aaataatgat tttcagcagc ttgtttcaat gcatctattg ttccattacc gataactttt
    12061 tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct actcttcaat
    12121 tccacaccaa tacccgtcac cgtaaaatct ggtttttctt cgatcctata ctttgccatt
    12181 tctcccaccc tttctattat attaagttca tcttacataa aattaaaggg aaatgacacg
    12241 attacgctct tttttctagg gatattttta ttttttaact atttttttgc tttcactgca
    12301 atccaaatct cactgtaaac attactgaaa tcctctcccc attttgtaaa agagatctcg
    12361 ggagcttcga ctaactcata atctgaagaa ggaagccatt ctgcatagat tttcccccaa
    12421 gtatcttgta aagtttgggg aaatggccct ttattaggaa aaatggccca agtgtgttct
    12481 tctatagcaa gcatttctaa atcagcgtac ggattttctt ttgtcgtagc aaagccaatc
    12541 atgtgcatca aactgccctt ttcttccaag cgggctccat caaatccata agaaacgttg
    12601 attacttgat ttggatataa atctcctaac ttatgcatct cttccctttg agtctccgtg
    12661 atcgtctgcg ctaacttttg tatttcttga ttgactcctt caaatttgac aggaactttt
    12721 ttcgccacac ctgccacata aaactgttct ttcttttcga tacgaaattc catcgatttt
    12781 cctcctctga catcaataaa aaatgtcaag cgtggaaaag atttttgtat gccgtatctg
    12841 ctgatttctg aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact
    12901 gtttcatatc catatctaaa cgccgtttct gtcactcttg atccatctag gagatcgcga
    12961 ttagccaaag ccaatctacg gtttttgata tagtccatta gcgacatccc tgcaataaac
    13021 gtaaagatgc gttttaattg atattcagag attcctgtat tttcggctat tattttatag
    13081 gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca attggttcag atctttaagc
    13141 atatttatct tcctccttgc ttgccttttt atcatattat caaagaaagt caattacttg
    13201 atgaatcagg aaccaattta gtcagatgta gaaaatgttg tatggatcta aagaaaattc
    13261 tcaagcagcg tttcgtaacg gataattggt attatctgcc tatctatgtt ttacctttag
    13321 aaaaataaat tttggggaag acacattcta agtaaacaaa gcggtaaaaa tatcttttat
    13381 tcccttagtt aatccgctta ctacctactg tacaaagcaa attgttgttc aaactgctga
    13441 tcagaacgct gcttggattt ttctttgcga tacatcatac ctcttttatc aattggaaca
    13501 attatttatt tctaac
//
LOCUS       DAFGFZ010000078        13433 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000078 DAFGFZ010000000
VERSION     DAFGFZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.080"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(93..635)
                     /locus_tag="LR726_001976"
     CDS             complement(93..635)
                     /locus_tag="LR726_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL6217062.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="LR726_001977"
     CDS             770..1159
                     /locus_tag="LR726_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217063.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="LR726_001978"
     CDS             1163..1927
                     /locus_tag="LR726_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL6217064.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2056..3003
                     /locus_tag="LR726_001979"
     CDS             2056..3003
                     /locus_tag="LR726_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217065.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3133..3873
                     /locus_tag="LR726_001980"
     CDS             3133..3873
                     /locus_tag="LR726_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217066.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4164..4832)
                     /locus_tag="LR726_001981"
     CDS             complement(4164..4832)
                     /locus_tag="LR726_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL6217067.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4966..5253
                     /locus_tag="LR726_001982"
     CDS             4966..5253
                     /locus_tag="LR726_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217068.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5669..7207
                     /locus_tag="LR726_001983"
     CDS             5669..7207
                     /locus_tag="LR726_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL6217069.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNAPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7204..8169
                     /locus_tag="LR726_001984"
     CDS             7204..8169
                     /locus_tag="LR726_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217070.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8156..9478
                     /locus_tag="LR726_001985"
     CDS             8156..9478
                     /locus_tag="LR726_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL6217071.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9541..9645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9719..10462
                     /locus_tag="LR726_001986"
     CDS             9719..10462
                     /locus_tag="LR726_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6217072.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10477..11469
                     /locus_tag="LR726_001987"
     CDS             10477..11469
                     /locus_tag="LR726_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6217073.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11481..12218
                     /locus_tag="LR726_001988"
     CDS             11481..12218
                     /locus_tag="LR726_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217074.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12208..12930
                     /locus_tag="LR726_001989"
     CDS             12208..12930
                     /locus_tag="LR726_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL6217075.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13014..>13433)
                     /locus_tag="LR726_001990"
     CDS             complement(13014..>13433)
                     /locus_tag="LR726_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL6217076.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4426 a         2296 c         2790 g         3921 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     3001 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3061 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3121 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3181 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3241 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3301 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3361 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3421 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3481 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3541 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3601 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3661 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3721 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3781 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3841 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3901 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa agtttatatc
     3961 gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta agaaaataat
     4021 ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta
     4081 taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt ctgtaaatta
     4141 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
     4201 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
     4261 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
     4321 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
     4381 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
     4441 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
     4501 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
     4561 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
     4621 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
     4681 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
     4741 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
     4801 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
     4861 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
     4921 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
     4981 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
     5041 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
     5101 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     5161 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     5221 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     5281 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     5341 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     5401 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     5461 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     5521 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     5581 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     5641 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     5701 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     5761 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     5821 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     5881 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     5941 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     6001 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     6061 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     6121 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     6181 tattgaccga aatgcacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     6241 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     6301 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     6361 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     6421 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     6481 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     6541 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     6601 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     6661 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     6721 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     6781 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     6841 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     6901 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     6961 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     7021 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     7081 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     7141 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     7201 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     7261 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     7321 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     7381 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     7441 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     7501 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     7561 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     7621 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     7681 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     7741 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     7801 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     7861 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     7921 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     7981 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     8041 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     8101 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     8161 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     8221 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     8281 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     8341 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     8401 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     8461 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     8521 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     8581 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     8641 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     8701 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     8761 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     8821 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     8881 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     8941 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     9001 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     9061 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     9121 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     9181 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     9241 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     9301 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     9361 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     9421 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     9481 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     9541 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     9601 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     9661 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     9721 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     9781 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     9841 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     9901 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     9961 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
    10021 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
    10081 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
    10141 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
    10201 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
    10261 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
    10321 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
    10381 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
    10441 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
    10501 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
    10561 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
    10621 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
    10681 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
    10741 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
    10801 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
    10861 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
    10921 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
    10981 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
    11041 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
    11101 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
    11161 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
    11221 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
    11281 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
    11341 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
    11401 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
    11461 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
    11521 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
    11581 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
    11641 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
    11701 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
    11761 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
    11821 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
    11881 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
    11941 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
    12001 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
    12061 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
    12121 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
    12181 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
    12241 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
    12301 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
    12361 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
    12421 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
    12481 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
    12541 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
    12601 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
    12661 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
    12721 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
    12781 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
    12841 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
    12901 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
    12961 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
    13021 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
    13081 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
    13141 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
    13201 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
    13261 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
    13321 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
    13381 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAFGFZ010000079        13408 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000079 DAFGFZ010000000
VERSION     DAFGFZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.081"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1166
                     /locus_tag="LR726_001991"
     CDS             <1..1166
                     /locus_tag="LR726_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBL6217077.1"
                     /translation="LKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPST
                     VIDFDIPKKYGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPM
                     AMLTMGVLKATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLL
                     EITKNGEDFYPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSK
                     YPELIDELGIPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTG
                     TPSVIAGNVLNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQ
                     ELTVEAALTLDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            1314..2573
                     /locus_tag="LR726_001992"
     CDS             1314..2573
                     /locus_tag="LR726_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL6217078.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            2583..3455
                     /locus_tag="LR726_001993"
     CDS             2583..3455
                     /locus_tag="LR726_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6217079.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            3467..4297
                     /locus_tag="LR726_001994"
     CDS             3467..4297
                     /locus_tag="LR726_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6217080.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            4337..6520
                     /locus_tag="LR726_001995"
     CDS             4337..6520
                     /locus_tag="LR726_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL6217081.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            6581..7801
                     /locus_tag="LR726_001996"
     CDS             6581..7801
                     /locus_tag="LR726_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBL6217082.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            7798..9258
                     /gene="gtfA"
                     /locus_tag="LR726_001997"
     CDS             7798..9258
                     /gene="gtfA"
                     /locus_tag="LR726_001997"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBL6217083.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            9335..10441
                     /locus_tag="LR726_001998"
                     /pseudo
     CDS             9335..10441
                     /locus_tag="LR726_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            10763..10972
                     /locus_tag="LR726_001999"
     CDS             10763..10972
                     /locus_tag="LR726_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL6217084.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            10965..11114
                     /locus_tag="LR726_002000"
     CDS             10965..11114
                     /locus_tag="LR726_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBL6217085.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            11268..11933
                     /locus_tag="LR726_002001"
     CDS             11268..11933
                     /locus_tag="LR726_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6217086.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(12217..13302)
                     /gene="xerS"
                     /locus_tag="LR726_002002"
     CDS             complement(12217..13302)
                     /gene="xerS"
                     /locus_tag="LR726_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL6217087.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         4547 a         2039 c         2575 g         4247 t
ORIGIN      
        1 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
       61 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
      121 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
      181 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
      241 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
      301 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
      361 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
      421 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
      481 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
      541 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
      601 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
      661 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
      721 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
      781 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
      841 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
      901 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
      961 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     1021 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     1081 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     1141 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
     1201 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
     1261 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
     1321 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
     1381 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
     1441 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
     1501 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
     1561 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
     1621 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
     1681 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
     1741 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
     1801 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
     1861 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
     1921 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
     1981 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
     2041 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
     2101 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
     2161 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
     2221 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
     2281 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
     2341 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
     2401 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
     2461 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
     2521 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
     2581 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
     2641 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
     2701 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
     2761 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
     2821 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
     2881 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
     2941 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
     3001 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
     3061 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
     3121 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
     3181 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
     3241 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
     3301 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
     3361 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
     3421 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
     3481 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
     3541 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
     3601 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
     3661 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
     3721 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
     3781 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
     3841 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
     3901 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
     3961 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
     4021 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
     4081 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
     4141 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
     4201 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
     4261 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
     4321 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
     4381 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
     4441 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
     4501 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
     4561 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
     4621 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
     4681 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
     4741 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
     4801 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
     4861 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
     4921 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
     4981 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
     5041 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
     5101 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
     5161 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
     5221 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
     5281 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
     5341 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
     5401 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
     5461 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
     5521 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
     5581 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
     5641 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
     5701 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
     5761 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
     5821 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
     5881 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
     5941 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
     6001 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
     6061 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
     6121 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
     6181 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
     6241 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
     6301 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
     6361 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
     6421 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
     6481 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
     6541 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
     6601 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
     6661 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
     6721 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
     6781 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
     6841 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
     6901 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
     6961 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
     7021 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
     7081 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
     7141 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
     7201 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
     7261 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
     7321 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
     7381 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
     7441 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
     7501 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
     7561 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
     7621 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
     7681 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
     7741 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
     7801 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
     7861 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
     7921 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
     7981 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
     8041 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
     8101 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
     8161 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
     8221 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
     8281 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
     8341 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
     8401 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
     8461 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
     8521 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
     8581 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
     8641 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
     8701 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
     8761 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
     8821 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
     8881 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
     8941 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
     9001 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
     9061 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
     9121 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
     9181 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
     9241 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
     9301 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
     9361 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
     9421 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
     9481 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
     9541 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
     9601 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
     9661 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
     9721 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
     9781 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
     9841 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
     9901 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
     9961 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    10021 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    10081 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    10141 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    10201 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    10261 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    10321 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    10381 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    10441 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    10501 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    10561 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    10621 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    10681 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    10741 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    10801 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    10861 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    10921 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    10981 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    11041 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    11101 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    11161 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    11221 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    11281 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    11341 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    11401 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    11461 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    11521 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    11581 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    11641 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    11701 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    11761 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    11821 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    11881 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    11941 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    12001 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    12061 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    12121 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    12181 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    12241 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    12301 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    12361 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    12421 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    12481 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    12541 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    12601 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    12661 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    12721 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    12781 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    12841 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    12901 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    12961 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    13021 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    13081 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    13141 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    13201 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    13261 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    13321 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    13381 ttggtatgct ctaaaattta ttaatttg
//
LOCUS       DAFGFZ010000080        13272 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000080 DAFGFZ010000000
VERSION     DAFGFZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.082"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            379..711
                     /locus_tag="LR726_002003"
     CDS             379..711
                     /locus_tag="LR726_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6217088.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1007..1162
                     /locus_tag="LR726_002004"
     CDS             1007..1162
                     /locus_tag="LR726_002004"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL6217089.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1438..2778
                     /locus_tag="LR726_002005"
                     /pseudo
     CDS             1438..2778
                     /locus_tag="LR726_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3019..4635)
                     /locus_tag="LR726_002006"
     CDS             complement(3019..4635)
                     /locus_tag="LR726_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL6217090.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEVCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4753..4971
                     /locus_tag="LR726_002007"
     CDS             4753..4971
                     /locus_tag="LR726_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217091.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5166..5627
                     /locus_tag="LR726_002008"
     CDS             5166..5627
                     /locus_tag="LR726_002008"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL6217092.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5820..6059)
                     /locus_tag="LR726_002009"
     CDS             complement(5820..6059)
                     /locus_tag="LR726_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217093.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6083..7606)
                     /locus_tag="LR726_002010"
     CDS             complement(6083..7606)
                     /locus_tag="LR726_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL6217094.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7624..7746)
                     /locus_tag="LR726_002011"
     CDS             complement(7624..7746)
                     /locus_tag="LR726_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL6217095.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7776..8759)
                     /locus_tag="LR726_002012"
     CDS             complement(7776..8759)
                     /locus_tag="LR726_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL6217096.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8783..8932)
                     /gene="rpmG"
                     /locus_tag="LR726_002013"
     CDS             complement(8783..8932)
                     /gene="rpmG"
                     /locus_tag="LR726_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL6217097.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8953..9330)
                     /locus_tag="LR726_002014"
     CDS             complement(8953..9330)
                     /locus_tag="LR726_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217098.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9362..9541)
                     /gene="rpmF"
                     /locus_tag="LR726_002015"
     CDS             complement(9362..9541)
                     /gene="rpmF"
                     /locus_tag="LR726_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL6217099.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9556..9825)
                     /gene="rpsN"
                     /locus_tag="LR726_002016"
     CDS             complement(9556..9825)
                     /gene="rpsN"
                     /locus_tag="LR726_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL6217100.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10348..12114)
                     /locus_tag="LR726_002017"
     CDS             complement(10348..12114)
                     /locus_tag="LR726_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217101.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12074..>13272)
                     /locus_tag="LR726_002018"
     CDS             complement(12074..>13272)
                     /locus_tag="LR726_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217102.1"
                     /translation="QESLDVLSSETVEYIRGIQVIKIFGAKVTSFKALNKAINDYAKF
                     AYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIF
                     VSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYK
                     EECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSK
                     DTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREH
                     TLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLIKE
                     KTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRL
                     DNEKFL"
BASE COUNT         4177 a         2583 c         2046 g         4466 t
ORIGIN      
        1 atgattttaa ttatcaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca
       61 gtctcactcc taaaattact ttatttacgt atgaagtggg caggaggtcg aatccagaac
      121 tgggctatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct
      181 tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa
      241 aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt
      301 gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac
      361 cggaaggaat gaactctatt gagaaaaaca tatgatcgta aatttaaact aaagatttca
      421 caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct
      481 cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct
      541 ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa
      601 cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga
      661 tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta
      721 tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag
      781 aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga taatttgaac agaaccaaat
      841 tgatgctgtt tgggtgatgg atattactta cattccatgt aatgacggtc gcctctattt
      901 gtccacctat attgacttag caactcgtat tctacgttac tatatggtag attcacacat
      961 gaaaaaagag attgtcgtcc gtccattaca aatttattaa ggagaattgc ctacggtaat
     1021 tcattccgat agaggtagcc agcatcggtc ttttatttat caagagctgt tgaacgacca
     1081 ccacatcaca cattcagtga gccaaactgg gactcctgtt gacaatgcag taattgaagc
     1141 gtttcatcga tcaatcaaat gagaattaat cgactccaac aaatataaaa gtaaaataga
     1201 gatgaaggtg ttaatccaag attatttaga agactactac ccaaacaaaa gaatccatac
     1261 aaaatttatg atgactcctt gacaatttga agcgaagaaa ataagggatc tataatatct
     1321 agtgttggtg acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga
     1381 caccttaaaa tcctttagaa taaagtcgac gaaaaccatt caaaggagtg tttcaagatg
     1441 tcctattcag agtttaccaa agagttacta gatattctag acctaaatct tatctttcat
     1501 gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat gttttgtttt tgatggcaca
     1561 ttgacctatc aaccggagga atgctttcat cgccactatc aaaatgaaca aacgatcatt
     1621 aaatagggct ggaaaaaagt atccatctta ttgaatgatg tcagtaacta caagaccatt
     1681 ctgcggatta acaaacagcg cttcaaatgt aaacactgtg gaaagacttt tttagcagaa
     1741 gattcagtta gtgatcgtcg ctgctccatt gctcgcagag tcaaacaagc cattcttgag
     1801 ctattaagcg aacctctttc tatgtctttg atcgcacgta tgaagcacat ttctccgacc
     1861 actgtcattc gaatacttcg cagccttcgg cctaaaacca tttctttgag ccagctgtta
     1921 ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc
     1981 tttgtcatga tggacggcaa gacccatcaa ctgattgata ttgtggaaaa tcggcaactc
     2041 aatcctttac gcgactattt cttgcgctac cctataaaag tccgtgaaca agtgcgtcta
     2101 gtggtttcag atttctatac accctatcgc acattggtga aagagtgttt tccgaatgcc
     2161 cgaatcgtcg ccgatcgctt tcatattagc caacacattg gtcgggcttt taccaaccac
     2221 cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa
     2281 tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt
     2341 ccgtcatttc gagatcatct gacagaagca gagatagtcg atcggctatt atcttatgat
     2401 gattccttaa agcgaggcta cgaagtctac caggattttc tttccgttat cagaagacaa
     2461 gatgttcctg aattcgtcgc gctcctaaaa gaagactaca aggagttgcc agagcactac
     2521 caaccagtgt tcacgacctt caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt
     2581 ccctattcca acggccctat agaatgtttg aataaccata tcaaagtact aaaacgaatt
     2641 gcctatggct atagaaactt tcaaaactac cgagaacgaa tctttttata tcgaggaaaa
     2701 tattttaaga aaactaagaa tactacccaa ctgactaaag ccagaacaac aacacggcta
     2761 gacaagatag ctgtctagcc gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa
     2821 cactcattga cgaagagcca aaataagtaa tctaaccgac tgttaactaa accattgcag
     2881 gtcccgttaa agatacttgt gcctgttgtc accgcttctt tttcttgtcc acaagcgctt
     2941 aacacactta ataaagcccc tgctattatt aataataata tctttttctt attaaaaacc
     3001 tcctcatcta tttccagatt agtaaatgta gtaatctgtg ctttcttttt tctcaatact
     3061 aataactaac ttatggggta aacaaatact cgtttgaccg ggttttgaga tccatccagt
     3121 tctaaccgcg atttgatcag gactattatc ttctttaact cgaattcttc catctttaat
     3181 ctcaacaata ttatattgtc ccttcgcagg atgataattc acgagtttat gattgatctt
     3241 atccaaatcg aagcgatcca tttccttgcc atcaattcga acgatggcat atttgatagc
     3301 atcttcagtt gtttcattct ccttttctat agagaataca aataagggaa taaaagaggt
     3361 aataatcaaa atgccaataa ttatgacatc gcctatcttg acaccagctt ttcttaaaaa
     3421 tttcattaac gatatctggc taaacatatt agactttttc catggaaatg gtgaaatttt
     3481 tcggaaatta tctgcattaa gagaatcagt taaacaaacc tcaagttgtt ccttaataca
     3541 ttgttgatcc atcttcatac caataaaaac tagtttatta atgcgttcac caaacaacgg
     3601 atgccaggct tccttcaatt cgggattttt ttgtataatc atttgacact caattttagg
     3661 taaactagct acccaataat ttaatgattc aatactttta tagctacccg atgtttcaaa
     3721 gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt
     3781 gccaggaaaa tttttatccg taagatgggc aaatttttgt gcgtcaagga gtaaccgatt
     3841 tcgatagata aaacttgaga taccatattc ttctgtttca ggaatatgat tttgatagcc
     3901 aagctctaac tctttgaacc agcccactga ataggaagtc tcatctatat taaaaagatt
     3961 tgtatttaga attttcgata agtctacttc acctttaatt gtcctaatta ttttggcatt
     4021 ggactgtaac ttagcaatta gaccgttaat tttgtaaatc gtcttggttg acactaagtc
     4081 actattattt aaaagtaata tgttacaaca ctcgaattga tcaattaaca agccacgaat
     4141 atcctgttct tcttcaacta cattaccttc agcttttctt tcctgaatct tttcacccgt
     4201 ttcgaaatta tcccaaaaac gttttacatc cactactata ataatggcac caatctctaa
     4261 tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat ataattgctt gaataattgg
     4321 aattggtttg ctgattcctg aagcttcaat aacaatttga ttcaagtcag aaaattgcac
     4381 taattgatct agttcaacaa tcaaatcttc gcgtaatgta caacaaatgc atccattcgg
     4441 catcgaaaca agtttttctc cagttctttt aaaacctttc ttgtcgacta ataccccatc
     4501 aacatttact tcccctatgt catttacaat gacagcaatt tcctttccgt gatcaccgta
     4561 cagaatatga tttagaagta tcgtttttcc tgaaccaaga tagccagtta agattgtaat
     4621 aggtattttt ttcataggga acctctttct ataccttgaa tttacacttt aagtgcaaca
     4681 cataaaaatg agtcataatc gatatccaac tagtataagt ttgtcgagaa cactcctaga
     4741 aggaaaataa ttatgagcca gtatcgtcat cttactatac tcgaacgaga aagaatcttt
     4801 ctgcttcacg aagaaggatt ttctattagt ataattggca acgacattca gcatagcccg
     4861 tttactattt tacatgaact gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg
     4921 aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta tcacattcta aatgatccgt
     4981 gagtttggtc aattgttcgt catttatttg tcgattttca gtggtcgcca gaggaaatct
     5041 cgaatcgttt gaagcatgaa catatggact tttcaattag ttttatcacg atctaacgtg
     5101 caatttgcaa tgatcttttc gatacagaac ccctctcaca cggcaataaa ggcctgatcc
     5161 gttcattgca ccatcgagga aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa
     5221 agatttgcct tactcattcg atccatggcc gtccaaaatc cgcagatgat agaagtgatt
     5281 ttggtcattg gaaagccgat attgtagctg gcaaaactgg tggtacttgt ttagtgactt
     5341 tgacagacag aaaatcatgc ttccttttag caggaaagaa ggtccccaga aagaaagccg
     5401 cttatgtgag cgagaaaatg attgaacttt taagcagtat tccaaatgag gcggtcaaaa
     5461 cgatcactcc agatagagga aaagaattct ccaatcattt cgcagttaca gaagcattga
     5521 ataatatgcc cttttatttt ccagatcctc atgcccttgg caacaatgaa caaattaaaa
     5581 tacgaataga ctcattcgag aatacttgcc aaaatcgaaa gagatagcta cggtagataa
     5641 ctcaacgata aatcagatga ttgaaaaact gaataacaga cctagaaagt gtttagattg
     5701 gagaacacct tatgaagtct tctttgataa agtgttgcgc ttgatttgac aattcaagac
     5761 ataataaaaa gcagatcaaa tccaaataag ggaaaaatga tctactctgg tattatcaat
     5821 taattggcca agctattatg ccgttttgct aaacgtctac ttcttccagt attgaccttg
     5881 ataaaacaac aatcaggatc ccgttttaat actctatgaa ctgtgtcaat actaacatgt
     5941 aaagttgcag caatatcagc taatgtactc ccatttaatc tcatttgctt tgcaaatcga
     6001 taacgccgat aagcatcaat attcttggaa tccatagaca ataatacttt tcttaccatg
     6061 tatttcctcc aaaaattgaa ttttaatgag ccatcatttc ttgagcaatt tcatgtttag
     6121 acaatgaagc tggatagaaa gtaggccaat tatgcaattc attatagagt ttttcttgac
     6181 tttctccacc aaagaaaata tggaaatgct ctgccttact tggtgcaatt ccatgatcac
     6241 taaattgaat atatttaaaa cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa
     6301 caccacggtt tcctttttca taattcaaaa ttttgtatcc aacatatttg tatgtgtatt
     6361 gatgatgttc accgttgact acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa
     6421 tatctgtctt atagccagta gtataataat ctttgtactc ggcagctgtc atgtctttat
     6481 tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc atcttttaac aacggataaa
     6541 cagattgcca ttcgcctgta taatcactca attcgcgatc cttcacatcg gcatctttaa
     6601 agtacccatt agcaactgtc ttttcatcct tcgcttcttt ttctggaact acctcttcac
     6661 cagcaaccat agttgttttt tctaacgctt ttagattgtc ttccattact gaaacataat
     6721 cttcaccatt atccatttgt tcttttgtaa gactttcaag aggattcagt acttccaatt
     6781 ttatcccagc ttcgttggcc aaagtttttg caattttatc attagcattc ttttcaaagt
     6841 aaatataatt gatcttattt tttttaacat actcttttaa ttcagctaat cgaccagaag
     6901 atggctcttc ttcaggactt agtccagcaa taggaacttg tttcaatcca taatccaaag
     6961 ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt ttgtttcgct tctttaagac
     7021 tagtggtata gtcagcatcc aaccgtttca gtttagtcaa atacttttca gcatttgtct
     7081 caaatacttt tgcttttttc ggatacaatt taactaactg atccttgata tttgaaactt
     7141 ccttaattgc cattttgggt gaaacccacg tatgaggatc tagttcatga tggtgatctt
     7201 cttctccgtg atcatgatct tcttcttcac tacctggcaa taaaatcatg tcttttgttc
     7261 cttctactac attaggccca gctttcttcc aactttcttt agcttttggt acccatgatt
     7321 ccatgttttc gttatgatat acaaaaacat cagcatcatg agcttttgcc atatccttag
     7381 ctgagggctc atagtcatga ggttctgatc ccgctggaac cattaaatcc acgttcgctt
     7441 catcgccgac gatgtttttg gtgaaatcat acattggata gaatgttgtt acaatttcta
     7501 attttccatc tttatcattc gatacttttg catcattagt gctggagcaa gctcccaata
     7561 atcctagagc tgctatccca ataagtccta cactgatttt cttcattctt tttccttcct
     7621 taattatgct aatttcttca ttttatttcg tttatgttct ttgataattt ttaatgccct
     7681 ttttctagtt ttaatattcg ggcttttaag tcgtcgatat gcactagata tatccgtttt
     7741 ctccattaga aattaccccc aatcactact tatcattatt tgctttccgc cagtaattta
     7801 tcaaaactct gtttgatcct ttcttcatcc aacatttttc cgattagaat aatagtggtt
     7861 tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca
     7921 actataaaat cctgctcttg taaagcaatc aatcctttaa aacgatacag gctcccttgg
     7981 ttggttaaaa tcagccagtc aatccattgg agtaaaaaat ctttttttat gtcttgatct
     8041 gaagttaaga gaattgattt aaactcatgt acttgatggt gttgatgata gtatttgcca
     8101 ttatagtgat tttctgatga atcactttca aaaactaagg gatgattaaa ttttttcaaa
     8161 ttataaaaat cctttttttg aatcgtttca ctatgtgtga acattcgaat atcggataaa
     8221 gggttaatac ttttgatttc tctctgtaat ttttttagtg cagataaatt caagttttgt
     8281 tttctagaca caaaaatgcg atctgccatg acaagttgct taagggcttg tggttctctt
     8341 tcaataagtt caaaactgtc actatcaata acagtcaata aactatcgat atatactctt
     8401 cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg gttggggatc tgctatacct
     8461 gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt cttctattgt ttccagtaaa
     8521 gaaatcaata ttgctaataa atcactgcga agactgcaac acatacatcc attatttagt
     8581 tgtaacaccc gttcctcgct atgaactaat aatttgtgat caatcccaac ttcaccaaac
     8641 tcattttcaa ttataataat ttcttccctg gggtagggtg agacttgcaa ggcttgattg
     8701 attagcgttg tttttccagc ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt
     8761 ttgtcacctt tttaccactt agctatttca cttctttaaa aattgcccgt ctgcgcaatt
     8821 ttggcgaata cttttttaat tctaaccgtt cagggttatt ccgtttattt ttgctggtga
     8881 gatacagacg ttctccagtt tcgacacatt ccaaaataat attttgacgc ataaatcttc
     8941 ctccttcaat acttatcgat cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt
     9001 taactcgcca gattccaatt gtgaaactaa attttctttc atatcaacag gtatctccca
     9061 ataaccatcg atacattgaa ataactcctc gtcatactct tcaaaaattg gatagattct
     9121 taaaaaatca tcaaatgata agccttggtc ctcatagtaa tctttgacta atccattttc
     9181 aattgcctgt tgggtcggta aacccgtttc agtaataatt cctctctcta ccatcgaacg
     9241 ataaaaagtg catttatcta gcgattgctg ggtcgtttca agataatact ttaatccttc
     9301 ttcaactaaa ggtgggtaaa taatttccat tttgattccc tcctaaaagt tatatattaa
     9361 actattttga agctccttta attactttct ttccattata gtaccctttt aaagatacat
     9421 gatggctacg tctataatca ccattcgctt cgtcaaatga aatttctggt tttactacag
     9481 tttgatgagt tcttcttaac cgtttcttcg ccttagatgt ttttcttgct ggtactgcca
     9541 tgatatttcc tccttttacc aacttgcttt ttttactcca ggaatcaacc cttgatgggc
     9601 aagttctcta aacttaatac gggacatacc gaactttcgc atataccctc ttggacgacc
     9661 atccgtctga tcacgaagcc tcagacgatt cggatttgaa tctcttggca atttagcgag
     9721 agcggtgcga tctcctgcgg cttttaattc ctgacgttta tctgcatact tttcaatcat
     9781 cgccatttgt ttctttgctt tagcaatttt cgactttttt gccatatacc tttatactcc
     9841 ttttattttg atttagacac ctcaaatcgt aatcgttacg ttttgacact gatactttac
     9901 caacaagagt tgctcttgtc aacaataaat aacatgaaat aaaactcata tgaatagata
     9961 actatttgaa aacgaattta tcggtacata gagataatca aatcatttaa aagaatatgc
    10021 agtttaggct ctgaaaaata agaattcact tttattagtc caatcaaaca caaataccgt
    10081 tttaagtagc ttttcctcta cttaaaacgg caatatgaaa gaaactataa ctttttattc
    10141 aatcttttat gtttaactgg tttatcgcca attttttaaa actttgatta ttccttatat
    10201 tataagaata gacattaagt gaactaattc aatcatactg attctttcgt gtattcaaaa
    10261 ttagatgctg tttaataaaa ttattctttt tgaaaaagaa ttataactat atcccaaaaa
    10321 ttgtaggtta atttaaagcc tatttcatca tcgtgattga gcttcttttt catagacgaa
    10381 tttctcagat agtttagttt tctttatgag atttttgtat atttctgacc ttcgatacaa
    10441 ctcattatga gtaccttgat caactagttt tccttgatcc atcactacaa ttttatttac
    10501 cttttcaata gacttcaagc gatgcgatat aattagtact gtcttatctt gtattaaacg
    10561 atttaaactt tcctgaatac gtttttcatt ttccgcatct aaactggcag taatttcatc
    10621 taaaattaaa attggcgcat ttttcaaaaa agcccgtgct atggataggc gttgtcgctc
    10681 tcctccagaa agtgaacttc cgttttctcc gaccaatgta tgataaccgt caggtagttt
    10741 ttcaataaag tcttcgcagt tagctaaacg agctgctttt ttcacttcct catctgacgc
    10801 ttgactattt ccaatacgta tattttccaa aatacttgta ttaaacaaag ttacctcttg
    10861 aaaaacaata gcgattttgc taaacaaaga agctggagat acacgtttaa tatcataccc
    10921 atcaattaaa atacagcctt catcataatc atacagtcta gacactaatt tcaagatact
    10981 agttttcccg gaaccactag cacctactaa cgcagtcact tctccttgtt tagccgtaaa
    11041 agagatatga tctaaaattg gggtattgtt atcatatgag aaagagacat ctcgcaactc
    11101 aatgtcaaaa gatttcagag gactatctga tccctcttgg atactggttt ccttcatagc
    11161 tctaattcgc tgtattttag gagctaaata aaatatttca agcactgctt ctttcataga
    11221 atcgaaggaa tcctttattt ttattgcggc tagtaagtaa ccaacgacgt agagtatagt
    11281 cacttcaccc gttaacagta agtgaacacc tactagaatg actactgcta aactaacata
    11341 tgagaaaata gaggataagg ccatgactga aaaagtactt aattctacct ttaaatgaat
    11401 cctttcactc tcttccattt ttttgaaaag tctgtcttga acttttttag atagattaaa
    11461 gctattaatc tcttgctgca attctatagt ttcttgaaat tcttccgagt tttctctcaa
    11521 agtatcgtaa tatttagtat tggctttcgt ttgggatttc tttgataaca agattaagac
    11581 aaaactaaat aacgttggca aaataacagc taatcccatt ttgacattac caaccaaaag
    11641 catcactgaa ataaaaggga aaaacagagc cataccgccg gctttaggta ttgcatgact
    11701 catcgcatgc tcaatacctt caacgtcaga catgatagtt tgagataaat ctgataaatt
    11761 atgttttgaa aaataggata atggtaaatt tgacaaatcg tctgcaattt gcactcttaa
    11821 atgcgcactt tctttataag tactattata taagttttca tactccctat ctaacaacca
    11881 atacatgaaa agcaaagtaa caaacgatac ggcaaaataa agccaacggg gctttgtatt
    11941 ttctaaaact aattcgtcta tcagcatcat tataatgaat gctggtacca tattaatgca
    12001 gtatacaaaa aaactagaaa tagaagcttt agttaaagct tctgcgccct gatcacttaa
    12061 agcaaatttg tttttataaa aacttttcat tgtcaagcct ccaatcattt gcattctcat
    12121 aattagtgac caattgttta tacacttgat gtttagccat taattcatta tgatttccac
    12181 gttctactat tttcccttct gacaagacaa gaatttcatc tacagattta atgctggata
    12241 atcgatgagc tatcataata actgtttttt cttttatcag atgttgaaaa gccttttgta
    12301 gttcatactc attatctgga tcaatagatg cactggcttc atccataata acaatctttg
    12361 catcttttaa aattgctctg gcaatagcta tacgttgttt ttctcctcct gacagataaa
    12421 ctcctttact accaattaag gtatgctctc tttcgggaaa tttttctaaa atcgattcgc
    12481 aaccggctaa tcttagcgcg tcaaatactt tacttgctgg cgcttgagga tttgctatag
    12541 aaacgttatc gtaaatactc tgattgaata gtttcgtatc ttgaaagaca aaagcaattg
    12601 tgttaataat tgtgtctttt gaatagtgat ttaaaggctt accaccaatt ttaatctgtc
    12661 ccttatccac attgtagaaa cctgaaatta gtttagctat cgtactcttt ccgcttcctg
    12721 attttcctac taatgcataa attctttttt gttctaaaac taagttgaaa ttttctaaaa
    12781 cacactcttc tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa tttttaatct
    12841 gtgtctcgtt cccatatttt agttggtctt gcttcatttc taaataaagg gagtctagtg
    12901 tttcgactgc atatcttgct tcaaacaagt gcatacttac atacattatt ctcatgaaag
    12961 aaacaaaaat aattccactt aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg
    13021 gagtggtaag agaagttata aaaaaggtta taggaactat caaaatgcaa actatgccaa
    13081 aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact caaagaatac ttatatgcaa
    13141 acttagcata atcgttaatt gctttattca aggctttaaa tgatgttact ttagcaccaa
    13201 atatctttat aacttgaatt ccgcggatat attctaccgt ttcagagctt aacacatcta
    13261 acgattcttg at
//
LOCUS       DAFGFZ010000081        13210 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000081 DAFGFZ010000000
VERSION     DAFGFZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.083"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            175..1914
                     /gene="yfcC"
                     /locus_tag="LR726_002019"
     CDS             175..1914
                     /gene="yfcC"
                     /locus_tag="LR726_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL6217103.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2071..3255
                     /gene="iadA"
                     /locus_tag="LR726_002020"
     CDS             2071..3255
                     /gene="iadA"
                     /locus_tag="LR726_002020"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL6217104.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3366..3569
                     /locus_tag="LR726_002021"
     CDS             3366..3569
                     /locus_tag="LR726_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL6217105.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3670..4332)
                     /locus_tag="LR726_002022"
     CDS             complement(3670..4332)
                     /locus_tag="LR726_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217106.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4345..5847)
                     /locus_tag="LR726_002023"
     CDS             complement(4345..5847)
                     /locus_tag="LR726_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6217107.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6395..8074
                     /locus_tag="LR726_002024"
     CDS             6395..8074
                     /locus_tag="LR726_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL6217108.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8201..8680
                     /locus_tag="LR726_002025"
     CDS             8201..8680
                     /locus_tag="LR726_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL6217109.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8899..9579
                     /locus_tag="LR726_002026"
     CDS             8899..9579
                     /locus_tag="LR726_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL6217110.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9587..10918
                     /locus_tag="LR726_002027"
     CDS             9587..10918
                     /locus_tag="LR726_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL6217111.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10933..11895
                     /locus_tag="LR726_002028"
     CDS             10933..11895
                     /locus_tag="LR726_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL6217112.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12006..12344
                     /locus_tag="LR726_002029"
     CDS             12006..12344
                     /locus_tag="LR726_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL6217113.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12423..13130
                     /locus_tag="LR726_002030"
     CDS             12423..13130
                     /locus_tag="LR726_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL6217114.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4218 a         2323 c         2892 g         3777 t
ORIGIN      
        1 aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa
       61 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
      121 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      181 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      241 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      301 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      361 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      421 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      481 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      541 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      601 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      661 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      721 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      781 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      841 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      901 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      961 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
     1021 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1081 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1141 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1201 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1261 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1321 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1381 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1441 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1501 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1561 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1621 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1681 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1741 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1801 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1861 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1921 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1981 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     2041 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2101 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2161 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2221 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2281 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2341 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2401 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2461 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2521 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2581 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2641 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2701 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2761 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2821 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2881 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2941 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     3001 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3061 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3121 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3181 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3241 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     3301 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     3361 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     3421 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     3481 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     3541 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     3601 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     3661 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     3721 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     3781 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     3841 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     3901 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     3961 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     4021 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     4081 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     4141 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     4201 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     4261 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     4321 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     4381 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     4441 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     4501 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     4561 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     4621 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     4681 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     4741 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     4801 aatgatcaga gtaagtatga taataccggc tacagtcact agtaaaacaa catttttaga
     4861 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     4921 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     4981 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     5041 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     5101 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     5161 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     5221 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     5281 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     5341 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     5401 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     5461 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     5521 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     5581 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     5641 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     5701 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     5761 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     5821 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     5881 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     5941 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     6001 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     6061 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     6121 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     6181 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     6241 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     6301 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     6361 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     6421 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     6481 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     6541 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     6601 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     6661 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     6721 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     6781 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     6841 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     6901 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     6961 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     7021 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     7081 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     7141 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     7201 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     7261 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     7321 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     7381 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     7441 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     7501 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     7561 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     7621 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     7681 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     7741 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     7801 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     7861 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     7921 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     7981 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     8041 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     8101 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     8161 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     8221 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     8281 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     8341 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     8401 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     8461 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     8521 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     8581 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     8641 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     8701 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     8761 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     8821 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     8881 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     8941 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     9001 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     9061 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     9121 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     9181 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     9241 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     9301 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     9361 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     9421 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     9481 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     9541 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     9601 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     9661 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     9721 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     9781 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     9841 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     9901 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     9961 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
    10021 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    10081 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    10141 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    10201 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    10261 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    10321 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    10381 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    10441 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    10501 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    10561 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    10621 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    10681 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    10741 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    10801 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    10861 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    10921 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    10981 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    11041 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    11101 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    11161 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    11221 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    11281 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    11341 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    11401 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    11461 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    11521 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    11581 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    11641 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    11701 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    11761 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    11821 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    11881 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    11941 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    12001 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    12061 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    12121 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    12181 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    12241 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    12301 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    12361 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    12421 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    12481 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    12541 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    12601 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    12661 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    12721 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    12781 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    12841 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    12901 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    12961 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    13021 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    13081 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    13141 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    13201 atttggaata
//
LOCUS       DAFGFZ010000082        13209 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000082 DAFGFZ010000000
VERSION     DAFGFZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.084"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            144..1013
                     /locus_tag="LR726_002031"
     CDS             144..1013
                     /locus_tag="LR726_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL6217115.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1222..2247
                     /locus_tag="LR726_002032"
     CDS             1222..2247
                     /locus_tag="LR726_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL6217116.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2286..3236
                     /gene="manA"
                     /locus_tag="LR726_002033"
     CDS             2286..3236
                     /gene="manA"
                     /locus_tag="LR726_002033"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL6217117.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3330..5033
                     /locus_tag="LR726_002034"
     CDS             3330..5033
                     /locus_tag="LR726_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6217118.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5035..7068
                     /locus_tag="LR726_002035"
     CDS             5035..7068
                     /locus_tag="LR726_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL6217119.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7079..7519
                     /locus_tag="LR726_002036"
     CDS             7079..7519
                     /locus_tag="LR726_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6217120.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7719..8846)
                     /locus_tag="LR726_002037"
     CDS             complement(7719..8846)
                     /locus_tag="LR726_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6217121.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8849..9526)
                     /locus_tag="LR726_002038"
     CDS             complement(8849..9526)
                     /locus_tag="LR726_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217122.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9790..11556
                     /locus_tag="LR726_002039"
     CDS             9790..11556
                     /locus_tag="LR726_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL6217123.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11571..12008
                     /locus_tag="LR726_002040"
     CDS             11571..12008
                     /locus_tag="LR726_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6217124.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12021..13172
                     /locus_tag="LR726_002041"
     CDS             12021..13172
                     /locus_tag="LR726_002041"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL6217125.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3697 t
ORIGIN      
        1 ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt
       61 tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct
      121 tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa
      181 aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg
      241 ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg
      301 gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca
      361 aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt
      421 gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg
      481 ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga
      541 acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag
      601 aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta aatgttacta
      661 acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag
      721 aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg
      781 gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga
      841 tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct
      901 tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt
      961 tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc
     1021 tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct
     1081 agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt
     1141 ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg
     1201 atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg
     1261 gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca
     1321 tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg
     1381 tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta
     1441 gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat
     1501 cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca
     1561 aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt
     1621 gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg
     1681 ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca
     1741 ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt tcccacagaa
     1801 caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg
     1861 gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc
     1921 caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt
     1981 gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac
     2041 ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg
     2101 tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat
     2161 cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg
     2221 aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt
     2281 gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa
     2341 gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg
     2401 cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa
     2461 gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt
     2521 ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag
     2581 atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca
     2641 tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac
     2701 tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa
     2761 aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga
     2821 ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag
     2881 atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc
     2941 ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca
     3001 cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt
     3061 tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg
     3121 ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt
     3181 ctgagtgggc ggtccaaggt gaactagcaa tcattgcgac tgaaccaggc aaatagttcc
     3241 cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc
     3301 catcaataaa tagaagtatg gtgaaaaaga tggaatacaa atggaaagca aacaacagca
     3361 gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc
     3421 caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg
     3481 gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg cttttaccca
     3541 tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg
     3601 caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga
     3661 ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc cacgtgaaat
     3721 tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta
     3781 tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg
     3841 cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag
     3901 aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag
     3961 gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg
     4021 gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa
     4081 atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat
     4141 ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt
     4201 tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg
     4261 tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca
     4321 gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag
     4381 agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca
     4441 tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga
     4501 aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata
     4561 accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag
     4621 tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac
     4681 gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca
     4741 cagaacaaaa aaagtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct
     4801 cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg
     4861 ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac
     4921 aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg
     4981 aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat
     5041 ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct
     5101 atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat
     5161 aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca
     5221 tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa
     5281 agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg
     5341 acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt
     5401 tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg
     5461 aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta
     5521 ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg
     5581 aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg
     5641 gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg
     5701 atgctgacat tgacgatgga gcgtgtgact aaaaatcatt tactgacaaa tgagacatat
     5761 aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc acggatcgct
     5821 tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat
     5881 gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct
     5941 tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa
     6001 gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt
     6061 ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa
     6121 gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa
     6181 gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa
     6241 agcaatatca gtattgcagg attttcgcca agtggattag cttctactag catgttggaa
     6301 ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat
     6361 ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca
     6421 ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag
     6481 ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc
     6541 aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc
     6601 aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa
     6661 acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt
     6721 catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg
     6781 gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg
     6841 aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta
     6901 gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt
     6961 ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc
     7021 gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat
     7081 gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc
     7141 gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca
     7201 atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc
     7261 acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc
     7321 tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt
     7381 ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga
     7441 catcgaccaa gtaatggcgc tcagtgatgc gaaaaccatt gaagaagtcc aaatgatttt
     7501 tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat
     7561 aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg
     7621 gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt
     7681 gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag
     7741 atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt caagatacca
     7801 ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca
     7861 ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat ccatccaatc
     7921 gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact
     7981 agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac
     8041 aacaacagga acagaaagct ctggaaaaga tactcataca tcggaaggat tcgtatctcc
     8101 tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt gattattact
     8161 agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg
     8221 gtaaaacgga tgctatgttt ctttaccata ctatccggta atagattgac tagagagatt
     8281 acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa
     8341 atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagggaga
     8401 tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc
     8461 aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt
     8521 cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct
     8581 gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca
     8641 aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag
     8701 ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat
     8761 gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga
     8821 ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta
     8881 aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt
     8941 tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct
     9001 ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt
     9061 tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga
     9121 catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct
     9181 tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag
     9241 tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga
     9301 aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat
     9361 aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg
     9421 gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac
     9481 gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa
     9541 tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta
     9601 ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga agttttctga
     9661 taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa
     9721 agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg
     9781 agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa
     9841 aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg
     9901 ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc tttggctacc
     9961 atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa
    10021 gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc
    10081 ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc
    10141 atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc
    10201 aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt
    10261 ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct
    10321 gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat
    10381 aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga
    10441 aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct
    10501 tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac
    10561 tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc
    10621 ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga
    10681 ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat
    10741 tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct
    10801 gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa
    10861 gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca
    10921 ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg
    10981 ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat
    11041 caagcaatca gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta
    11101 caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg
    11161 aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt
    11221 tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa
    11281 gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc
    11341 aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat
    11401 ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag
    11461 atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac
    11521 gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt
    11581 tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct
    11641 attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc gatgccatgg
    11701 tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa
    11761 cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg
    11821 gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag
    11881 gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg
    11941 acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa
    12001 ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag
    12061 gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc
    12121 acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat
    12181 caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa
    12241 aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct
    12301 taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca
    12361 caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa
    12421 aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa
    12481 atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag
    12541 tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat
    12601 taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg
    12661 tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg
    12721 atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag
    12781 gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa
    12841 aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga
    12901 caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag
    12961 aacataactt gacttgccca catttgacgg caacaatggg gattattttt aattactatg
    13021 atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac
    13081 aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa
    13141 acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac
    13201 gaaattttt
//
LOCUS       DAFGFZ010000083        12926 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000083 DAFGFZ010000000
VERSION     DAFGFZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.085"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(287..649)
                     /locus_tag="LR726_002042"
     CDS             complement(287..649)
                     /locus_tag="LR726_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217126.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="LR726_002043"
     CDS             complement(723..1103)
                     /locus_tag="LR726_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL6217127.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="LR726_002044"
     CDS             1167..2360
                     /locus_tag="LR726_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL6217128.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2436..3020)
                     /locus_tag="LR726_002045"
     CDS             complement(2436..3020)
                     /locus_tag="LR726_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL6217129.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3221..4219
                     /locus_tag="LR726_002046"
     CDS             3221..4219
                     /locus_tag="LR726_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL6217130.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4331..4840
                     /locus_tag="LR726_002047"
     CDS             4331..4840
                     /locus_tag="LR726_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL6217131.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4870..5529)
                     /locus_tag="LR726_002048"
     CDS             complement(4870..5529)
                     /locus_tag="LR726_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL6217132.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5531..6295)
                     /locus_tag="LR726_002049"
     CDS             complement(5531..6295)
                     /locus_tag="LR726_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL6217133.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6303..6980)
                     /locus_tag="LR726_002050"
     CDS             complement(6303..6980)
                     /locus_tag="LR726_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL6217134.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7017..8402)
                     /locus_tag="LR726_002051"
     CDS             complement(7017..8402)
                     /locus_tag="LR726_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL6217135.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8612..8696)
                     /gene="ffs"
                     /locus_tag="LR726_002052"
     ncRNA           complement(8612..8696)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LR726_002052"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="LR726_002053"
     CDS             complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="LR726_002053"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL6217136.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="LR726_002054"
     CDS             9341..10198
                     /locus_tag="LR726_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217137.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="LR726_002055"
     CDS             complement(10279..10614)
                     /locus_tag="LR726_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217138.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="LR726_002056"
     CDS             complement(10681..12051)
                     /locus_tag="LR726_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL6217139.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="LR726_002057"
     CDS             complement(12129..12665)
                     /locus_tag="LR726_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6217140.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3884 a         2611 c         2141 g         4290 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca taataataat
     3061 aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact
     3121 agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct
     3181 caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac
     3241 aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc
     3301 taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc
     3361 cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga
     3421 tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct
     3481 ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc
     3541 caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga
     3601 taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta
     3661 tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat
     3721 gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca
     3781 cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat
     3841 cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag
     3901 tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa
     3961 tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc
     4021 agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg
     4081 aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt
     4141 acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa
     4201 aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca
     4261 gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg
     4321 gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa
     4381 ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta
     4441 tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg
     4501 tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa
     4561 attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga
     4621 gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta
     4681 tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt
     4741 ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc
     4801 acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa
     4861 aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt ttcctaagaa
     4921 aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc tatgcatgaa
     4981 aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata accagtcatc
     5041 gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa
     5101 aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca accgccaaag
     5161 ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag tgatgaaaaa
     5221 cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg ctccgctagc
     5281 agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga gataattcat
     5341 taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg
     5401 caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac caactgccag
     5461 actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact gccatctcga
     5521 atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta gctgggatag
     5581 gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa gtatctatat
     5641 tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg attacttctt
     5701 ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct ggttttccga
     5761 tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac cctgctccag
     5821 gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg aaagtagcac
     5881 ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa ttatctaagc
     5941 ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct aaaatcaagt
     6001 caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct gctttcataa
     6061 aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga atgtcaaact
     6121 catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg gtaacaaaca
     6181 agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt
     6241 gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag tccattcatc
     6301 cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac ttgtttaggt
     6361 tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg atttttaata
     6421 accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata cgcacgatta
     6481 tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg
     6541 atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg
     6601 attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt
     6661 tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt
     6721 tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt
     6781 tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct
     6841 gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt
     6901 tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt
     6961 tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat
     7021 ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac
     7081 aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga
     7141 tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact
     7201 tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg
     7261 cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct
     7321 aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc
     7381 ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac
     7441 agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct
     7501 acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct
     7561 acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca
     7621 tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt
     7681 tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg
     7741 gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat
     7801 cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg
     7861 agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc
     7921 aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga
     7981 gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt
     8041 ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca
     8101 aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg
     8161 tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca
     8221 ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc
     8281 gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc
     8341 cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc
     8401 atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga
     8461 aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg
     8521 ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa
     8581 aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct
     8641 tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc
     8701 ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt
     8761 tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat
     8821 gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt
     8881 accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa
     8941 aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc
     9001 caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt
     9061 agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa
     9121 tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa
     9181 tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc
     9241 ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac
     9301 ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatcg
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg gtactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcac
//
LOCUS       DAFGFZ010000084        12744 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000084 DAFGFZ010000000
VERSION     DAFGFZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.086"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LR726_002058"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LR726_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL6217141.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="LR726_002059"
     CDS             complement(322..891)
                     /locus_tag="LR726_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL6217142.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="LR726_002060"
     CDS             1077..1979
                     /locus_tag="LR726_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL6217143.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LR726_002061"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LR726_002061"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL6217144.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LR726_002062"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LR726_002062"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL6217145.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="LR726_002063"
     CDS             complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="LR726_002063"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL6217146.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8553..8738
                     /locus_tag="LR726_002064"
     CDS             8553..8738
                     /locus_tag="LR726_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL6217147.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8800..9996)
                     /locus_tag="LR726_002065"
     CDS             complement(8800..9996)
                     /locus_tag="LR726_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL6217148.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10166..11602)
                     /locus_tag="LR726_002066"
     CDS             complement(10166..11602)
                     /locus_tag="LR726_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL6217149.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11895..12704
                     /locus_tag="LR726_002067"
     CDS             11895..12704
                     /locus_tag="LR726_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL6217150.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3805 a         2675 c         2249 g         4015 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttagcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tactcttgct gtcatccacc caataagcat ctgacagaac
     5101 tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg ggtgtttctg
     5161 aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg taatacgcaa
     5221 atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa agctttctcc
     5281 aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa caaagatcac
     5341 tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa ctgtgacaga
     5401 aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt tttttgtccg
     5461 aatttctcta acttccttga tcatcactaa taaattgact ggttgattag ggacgatatc
     5521 tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg caggatgacc
     5581 tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta gtgtatagtc
     5641 agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc caccactgta
     5701 aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt taggagataa
     5761 ttcatcaaaa actcctactg ctgtcagtga ttgcagtaac tctaatttca gccaacgctt
     5821 atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt ttctttcgtt
     5881 taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc cgaatctgat
     5941 ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg gcgaggaaag
     6001 tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg tgttatgccg
     6061 aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta gatacgccat
     6121 ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat agtttgcaaa
     6181 tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct gttgtaatgc
     6241 tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt tactcactgc
     6301 ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg cgatctgcat
     6361 gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga tcagtgaatc
     6421 atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat ctatattttg
     6481 catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt tttctggatc
     6541 taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc ctgaggtttc
     6601 gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt ttaatcggat
     6661 ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg ataagttgcg
     6721 tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca tcgtatactg
     6781 ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta gattcttatc
     6841 gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt ctaaaatcac
     6901 tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta ctaattgatg
     6961 cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg cttttgacca
     7021 gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa gaaccatttt
     7081 tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat acttttctcg
     7141 aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga tatctggcat
     7201 cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta atggatccac
     7261 atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac gacctgcacc
     7321 ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca agaaataatc
     7381 atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt ggtattcctt
     7441 tgtatagtca gtgattcggc gttccacttg cgaatagcat aactctctta aatattctcc
     7501 agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga actggaattc
     7561 aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa gacccgcttt
     7621 ttcgaatcgt ttgatcattt cttgttctga aagaagatag acatttgctg ttagtggtaa
     7681 agatgcagat aaattttcta aaggtatttg gcttccctct ttgatatgtt tcatcacttc
     7741 aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac aaagcgaata
     7801 attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt tttctgtcaa
     7861 gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat gaccgaaaaa
     7921 gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa caaacgctag
     7981 atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt ttcggctgga
     8041 aagtttcatc agctgctgat aacctatttc atcttttgcg tacgcaagca agccataatt
     8101 tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc cttcttctac
     8161 acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta tggctaatga
     8221 agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg tactttgtaa
     8281 aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt ccgccttttt
     8341 tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt aagatagcag
     8401 gcgctgaagt tgattattcc acttcatttg cttgcttgta atctgtagga atgagttctt
     8461 tcgcaagaac tcaaatttta ttgagatttg gtctcgtttc tttacattca aagaaaatct
     8521 ggtaaaatta agatagaaaa gaggtgttct ttatgcgata tatcgttaca ttattctggg
     8581 ctgtcgttct aggtcaagta gtcggttata ttggtgctgc attaacaagc ggaacatatg
     8641 attttacttt gacaacaatc atttcattca ttgcgggtgt cattatcctt ttgattggtg
     8701 cagttgcgcc aagaaaagaa acctctgccc attcgtaaaa aaacagccaa gagcttgaga
     8761 tttctcaagc tcttttttat tttctactcc tcaccaccat tataatctgc tgcgaagaac
     8821 tttacgaaag ttgaataaag aaagaagaat atttgacaga acaaacaaac ttgtcacaat
     8881 aaaaacagca ctgtaattat aagcatgcgc gactgtcgat cctagcatgg gacctaaaac
     8941 ttgtccaaag ttgctaaaca tctgattgta gctatagatt cggctgactc cttcttgagg
     9001 cgtaatctta ctgatcagtg tattgacaga aggcatcaaa gcgccagttg aaaagcctaa
     9061 aataaagcga agtataccta attggagcgg tgtagtgacg aatgccatag gtaaataaca
     9121 gcaaaatgat aaaatgagtc ctccaattag aactttttga ttcccaatct tatcccctag
     9181 ttttcctagt ttcggtgaag aaaagacagc ggaaacacca gctatcgaaa caatcaagcc
     9241 gctgacaaaa aggatatttt ctgtacttcc gctcaattca cgaatatata aggtcaaaat
     9301 agggctgatc gtagtgattc ctatctgtaa aatcaaggtc gtaatgaaga gaccgatcaa
     9361 aatagagaga tgatccattt ttgaaaaaat ttctttggtg cttatcagat cttttttctc
     9421 gattggatgg aaatcttctt tgactaagaa aatggtcaat accgttgtga tcatcaacaa
     9481 agcccctgtg atcaagaata cattttccat accaaaccac tgagccagtg caccgcccat
     9541 tgaaggtccg atcagatttc cagcaatcgc tccggtagac aatgttccta acgcccatcc
     9601 gctcttttct ctaggtgcct gtgaagcaat catcgctgtg gcatttggaa tatatccaga
     9661 caaaatccca ttccaaaacc gcatgatcaa aagccaatac gcgtttggta caaaagccaa
     9721 agatcccatt gttaatgtca tccctgctgc tgcacggatc atcatgagtt ttcggccttt
     9781 tcgatcagct agatttcccc agattggtgc aaccacggca gatgcaaaag cagtcacaga
     9841 gattgctagt cccgaaaaca attcgatttg atcttttggt gttcctagtt gctcaacata
     9901 aactggtatg aatggcataa ctaaactaat acttgcacca gtgaaaaaac agccaatcca
     9961 cgcaatcact aaattttttc tccaattaat ctccatctat catccctctt tcgtctcttt
    10021 tatcatacct attttcgaat aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc
    10081 gcaaaggtca ttttatatgt aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa
    10141 tactgttcca atcccttctc tttagttaaa ttgttgctag aatgatgaag ttgataatga
    10201 ataaaatatc aacgatccac aaagcaacag aaacttgatt gtattttcct ttgattactt
    10261 tcacaattgt atagaaaata aatcctgctg caatcccata ggaaatgcta tagcacaatc
    10321 ccataaagat cgatgcaaag aaggccggta aagcttcttc catatctagc catttgatgt
    10381 ctgcaaaaga agccatcatc attactccaa ctaaaatcaa tgctggcgca gttgcttgat
    10441 ttggtacaat ggcgatcagt ggagagaata aactacttaa tgcgaatagc actgccacta
    10501 ctactgaagt caaaccagta cgtccaccgg ccccgatccc agctgcagat tcgacataag
    10561 ttgttgtatt cgatgtacca aagatagccc ccacagaagt agcgatcgca tcggcaaata
    10621 gtgctttatc catttttgtt ttgaacccac gaccatcttc caacgcgatc tcgtcttctt
    10681 ttgagaaaat acctgtgcgg cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat
    10741 cagataaact gaatgcgatg atcgtcatca atacttgagg aatcttggaa gaatcactga
    10801 ataaagattg catgccttca gaaccaaatg ctgcaccgaa agttgttcct aattctttaa
    10861 atgaattacc taacgaattc gtttgccaat caatggaact taaatcaaca acacccataa
    10921 aaataccaag gactgtggtc gctacgatac cgataagaat agccccacgg acatttttta
    10981 caactaaaat ggctgtcagt accaaaccaa tcacagccaa taagatagga gcgttgtcaa
    11041 agttggctaa cgcagggacg attccaccat ttatcgtaac atttgtcgct tttccgcctt
    11101 caacgacaga gctcgaaata gctgactggt ctgctgaaaa agataagaat cctgcatttt
    11161 tgattccgac atacgcaacg aaaataccga tcccgccacc aatcgcatgt tgaagacttt
    11221 cgggaatcgc ccgaataatc atttttcgaa tatttgtgac agtaataaaa atattgatta
    11281 atccgcaaat aaacaccatt gccaaggctt gttgccaaga ataaccaagc ccaaaaacga
    11341 ccgtaaaagt gaaaaatgcg ttcaatccca taccaggtgc ctgagcatat ggtacatttg
    11401 cgaacaatcc cattaccagc gtaccaataa tcgatgcaat gattgtcgct agaaatactg
    11461 cttggaaggg catacctgat gctgacaaaa tcgtcggatt tacgaataag atataactca
    11521 tggcaaagaa tgtcgttact cccgccatta tttcagttga tacatttgta ttgtttttct
    11581 ttaaaccgaa aaacttttcc ataaaaagtt ccactccctg aatttttttc tgatggaaaa
    11641 ctataataat aaaaaataag taaaataggg caaatccatc gaacaatttc aaattatata
    11701 gacgtttcat tcgcttttca acgttttttt aacaaaaaaa gcgaaaataa tacttaacgt
    11761 tcgtgtttag cttatgttta gctatttata tagccataac tgtagttttt gttaggaata
    11821 gctgattgct cttgttaatt acctaaaata tgctagacta aaacaaatgc tagttgagga
    11881 aggaaggacc cttattgaat aaaaaattaa ttgccatcga tctggacgga actaccctca
    11941 atcaagattc gatgattaca tctaaaacaa aagagactct aaaaaaagca atcaatgccg
    12001 gtcatcatgt cagtattgct acaggacgcc cattccgaat gagccatcag ttttaccaac
    12061 aattagaatt aacaacaccg atggttaatt ttaacggcgc attagtacac atccctaatc
    12121 agcactggga tggtgaaaga gaaacgttga tcaatcgtga aatcgctttt gaaatcttat
    12181 cgcaaaaaaa gcagctaaat ttagatttta tcgctgctga aaatcgtgat acttttttta
    12241 ttgacagttt tgatttcttt gatgaaaaaa tttttgcttc ttctcgtcca ggagagaaga
    12301 atttgctttc tcctaaaaat ttgacgacaa atcctacatc tttgcttgtc cgaacagaca
    12361 aacgttttgc aggagctgta tctgctgaat taacacgtca atttggcagt tatgtcgatg
    12421 ttcggacatg gggtggacct actgccatct tagagattgt ttcaaaagga atccaaaaag
    12481 caaaaggcgt gcaagagatt gctaactacc tgtctatcga tcaaaaagac gtcattgcct
    12541 ttggtgacga gcataatgat ttggaattgt tggattatgc aggttgggga gtagctatgg
    12601 ctaacggtac tgaccaactc aaaggcattg caaatgatgt cactcctttg agcaatcagg
    12661 aagacggttt agctgtttat ctggaaaaac tacttaaact ataatagaga attagattcc
    12721 acaaaaaaga ggttgtgaca attt
//
LOCUS       DAFGFZ010000085        12334 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000085 DAFGFZ010000000
VERSION     DAFGFZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.087"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            32..1660
                     /gene="treC"
                     /locus_tag="LR726_002068"
     CDS             32..1660
                     /gene="treC"
                     /locus_tag="LR726_002068"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL6217151.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(1720..2901)
                     /locus_tag="LR726_002069"
     CDS             complement(1720..2901)
                     /locus_tag="LR726_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL6217152.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3153..4676
                     /locus_tag="LR726_002070"
     CDS             3153..4676
                     /locus_tag="LR726_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL6217153.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(4745..5473)
                     /locus_tag="LR726_002071"
     CDS             complement(4745..5473)
                     /locus_tag="LR726_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6217154.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(5486..6766)
                     /locus_tag="LR726_002072"
     CDS             complement(5486..6766)
                     /locus_tag="LR726_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL6217155.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(6907..7509)
                     /locus_tag="LR726_002073"
     CDS             complement(6907..7509)
                     /locus_tag="LR726_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL6217156.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7754..7960
                     /locus_tag="LR726_002074"
     CDS             7754..7960
                     /locus_tag="LR726_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217157.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            8272..8991
                     /locus_tag="LR726_002075"
     CDS             8272..8991
                     /locus_tag="LR726_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL6217158.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(9072..9755)
                     /locus_tag="LR726_002076"
     CDS             complement(9072..9755)
                     /locus_tag="LR726_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL6217159.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            9973..12159
                     /locus_tag="LR726_002077"
     CDS             9973..12159
                     /locus_tag="LR726_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL6217160.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         4453 a         2115 c         2379 g         3387 t
ORIGIN      
        1 taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag tcatttacca
       61 aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt taaaaggaat
      121 caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc taaaccctat
      181 ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag ccatcgatcc
      241 tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga aataccggat
      301 agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa
      361 agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag agccaacgga
      421 ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg ggaaatatta
      481 tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag cagtaagaga
      541 ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga
      601 tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa atgatggcaa
      661 acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga accaagcaac
      721 gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga cgatcgaaaa
      781 ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta atttccatca
      841 tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg attttgcaga
      901 attaaagcga ctatttcata catggggaga aaagatgagt gagggtaacg gctggaatgc
      961 tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag ctatcgatca
     1021 gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc gtgggacgcc
     1081 ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat cgatttccga
     1141 ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag gattcacaga
     1201 aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa cacccatgca
     1261 atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa aagttggagg
     1321 caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta ttttgtccta
     1381 ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag gagattatca
     1441 tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg aaggacagca
     1501 gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc cagaaggatt
     1561 cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc aaagtctgga
     1621 gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg
     1681 ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat aatcttcttc
     1741 agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt agcgttgttt
     1801 gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag gaataccaaa
     1861 aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta agatcgtaat
     1921 cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac cgtggagaag
     1981 ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt cttggaatgc
     2041 aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga tacataacaa
     2101 cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga aacaaaccca
     2161 gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg aacccaattg
     2221 ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg ggacaatcgc
     2281 taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga caattggacc
     2341 agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga tccgttgtgc
     2401 gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt cgtcaataga
     2461 agcgaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt gtcggagact
     2521 ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg cctgttcttc
     2581 aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa tcacataaac
     2641 gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa
     2701 aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga taatgattcc
     2761 gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg caagtaagat
     2821 aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca aacgattcgt
     2881 tgtatctgtt tcttttttca ctctttttcc tcctttatct ataaattcat tatagacaaa
     2941 agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa tcattcatat
     3001 aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc atatgataaa
     3061 ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc ggaacattca
     3121 aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt atattggcca
     3181 tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc gaggacagta
     3241 aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca aaagcaatcg
     3301 tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc cacgattatg
     3361 aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt tataatgatg
     3421 aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc aatgtccata
     3481 cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga catgatcatg
     3541 accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta tggctagctc
     3601 caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa gcttacccag
     3661 aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt caagcactcc
     3721 atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc acgcaacatg
     3781 ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt gctggtcttt
     3841 cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt gttaaagaac
     3901 atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc agaacgttag
     3961 caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg acgaatgaac
     4021 aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa
     4081 aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa gaaaagagcg
     4141 tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca gattgggcag
     4201 gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct gtttttgaat
     4261 ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat tatacgaagg
     4321 gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca tttacgttta
     4381 agaatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc ttgacctacg
     4441 aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa agtggcgctt
     4501 ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa cattttcatt
     4561 tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat
     4621 tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg
     4681 caaaaaagaa ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt
     4741 ctttttatga aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg
     4801 ccaacgtgac ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat
     4861 aggatttatg acaacggata aaattccgat gcattttctc gatttctttg atcttctcat
     4921 agaattgtaa ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag
     4981 tagaaaaata ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt
     5041 tgaagatatc tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat
     5101 gctcgacatt ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga
     5161 tgttgctttt taagattttc atcgacattt ccgtatggga agtcacatag attatttttc
     5221 ctaaaggatc taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt
     5281 ctagttcgat atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga
     5341 gaagttcttc cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga
     5401 ttctattttt gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat
     5461 aaatgggaat catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac
     5521 gctggttttc aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca
     5581 agataaacaa gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt
     5641 tcaactggtt taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat
     5701 gattcttctt gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa
     5761 tcgcattgtc caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca
     5821 gttcgattgg ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga
     5881 gttttaatga cagtaaacta cggatctcag gaactttcat attatccaac atacttagat
     5941 tggccggaaa aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc
     6001 gtaatccttc taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt
     6061 aatcatgttt gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct
     6121 gttgcatcct gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc
     6181 aataataaac caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca
     6241 aagctaagac atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg
     6301 taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag
     6361 tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa
     6421 tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat
     6481 cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt
     6541 aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag
     6601 caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa
     6661 tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac
     6721 aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc
     6781 attgattttt accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt
     6841 ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt
     6901 aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt
     6961 aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta
     7021 agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt
     7081 taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata
     7141 gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt
     7201 caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag
     7261 acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga
     7321 aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac
     7381 cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac
     7441 catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt
     7501 atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc
     7561 ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt
     7621 cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg
     7681 acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga
     7741 tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa
     7801 atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt
     7861 gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg
     7921 gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga
     7981 gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa
     8041 cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt
     8101 ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga
     8161 gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt
     8221 atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata
     8281 attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt
     8341 gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg
     8401 ctattttatt ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa
     8461 cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc
     8521 tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa
     8581 gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca
     8641 caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga
     8701 atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac
     8761 tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa
     8821 atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc
     8881 gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc
     8941 cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac
     9001 taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg
     9061 attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc
     9121 tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac
     9181 agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga
     9241 tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc
     9301 gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt
     9361 ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg
     9421 ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga
     9481 acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt
     9541 agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat
     9601 tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa
     9661 gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt
     9721 cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat
     9781 ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta
     9841 aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag
     9901 acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag
     9961 gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg
    10021 gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc
    10081 tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa
    10141 agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat
    10201 caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga
    10261 gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa
    10321 caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta
    10381 aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat
    10441 agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa
    10501 ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag
    10561 ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct
    10621 cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa
    10681 gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg actgtatgga
    10741 ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta
    10801 ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa
    10861 tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg
    10921 aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt
    10981 atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct
    11041 accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca
    11101 aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa
    11161 aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg
    11221 ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata
    11281 ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt
    11341 ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa
    11401 catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag
    11461 gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc
    11521 gatcaatact tttctcctaa atatcaaaat atagccgttg cagattatat tttgtcgata
    11581 agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa
    11641 acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata
    11701 gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct
    11761 actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa
    11821 acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac
    11881 aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg
    11941 ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca
    12001 gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat
    12061 cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt
    12121 atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga
    12181 agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa atttttgtga
    12241 aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg aaaagggagg
    12301 tgtgacaaga attttgtcac acctccctta ttta
//
LOCUS       DAFGFZ010000086        12180 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000086 DAFGFZ010000000
VERSION     DAFGFZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.088"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1391
                     /locus_tag="LR726_002078"
     rRNA            <1..1391
                     /locus_tag="LR726_002078"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1486..1601
                     /gene="rrf"
                     /locus_tag="LR726_002079"
     rRNA            1486..1601
                     /gene="rrf"
                     /locus_tag="LR726_002079"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1615..1687
                     /locus_tag="LR726_002080"
     tRNA            1615..1687
                     /locus_tag="LR726_002080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1648..1650,aa:Val,seq:tac)
     gene            1691..1763
                     /locus_tag="LR726_002081"
     tRNA            1691..1763
                     /locus_tag="LR726_002081"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Lys,seq:ttt)
     gene            1771..1852
                     /locus_tag="LR726_002082"
     tRNA            1771..1852
                     /locus_tag="LR726_002082"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1805..1807,aa:Leu,seq:tag)
     gene            1870..1942
                     /locus_tag="LR726_002083"
     tRNA            1870..1942
                     /locus_tag="LR726_002083"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1903..1905,aa:Thr,seq:tgt)
     gene            1954..2025
                     /locus_tag="LR726_002084"
     tRNA            1954..2025
                     /locus_tag="LR726_002084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1986..1988,aa:Gly,seq:gcc)
     gene            2042..2127
                     /locus_tag="LR726_002085"
     tRNA            2042..2127
                     /locus_tag="LR726_002085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2076..2078,aa:Leu,seq:taa)
     gene            2138..2211
                     /locus_tag="LR726_002086"
     tRNA            2138..2211
                     /locus_tag="LR726_002086"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2172..2174,aa:Arg,seq:acg)
     gene            2221..2294
                     /locus_tag="LR726_002087"
     tRNA            2221..2294
                     /locus_tag="LR726_002087"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2255..2257,aa:Pro,seq:tgg)
     gene            2314..2386
                     /locus_tag="LR726_002088"
     tRNA            2314..2386
                     /locus_tag="LR726_002088"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2347..2349,aa:Ala,seq:tgc)
     gene            2414..2487
                     /locus_tag="LR726_002089"
     tRNA            2414..2487
                     /locus_tag="LR726_002089"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2448..2450,aa:Met,seq:cat)
     gene            2501..2574
                     /locus_tag="LR726_002090"
     tRNA            2501..2574
                     /locus_tag="LR726_002090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2535..2537,aa:Ile,seq:cat)
     gene            2598..2687
                     /locus_tag="LR726_002091"
     tRNA            2598..2687
                     /locus_tag="LR726_002091"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2634..2636,aa:Ser,seq:tga)
     gene            2700..2773
                     /locus_tag="LR726_002092"
     tRNA            2700..2773
                     /locus_tag="LR726_002092"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2734..2736,aa:Met,seq:cat)
     gene            2792..2867
                     /locus_tag="LR726_002093"
     tRNA            2792..2867
                     /locus_tag="LR726_002093"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2825..2827,aa:Asp,seq:gtc)
     gene            2869..2939
                     /locus_tag="LR726_002094"
     tRNA            2869..2939
                     /locus_tag="LR726_002094"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2901..2903,aa:Gly,seq:tcc)
     gene            2953..3026
                     /locus_tag="LR726_002095"
     tRNA            2953..3026
                     /locus_tag="LR726_002095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2987..2989,aa:Ile,seq:gat)
     gene            3051..3138
                     /locus_tag="LR726_002096"
     tRNA            3051..3138
                     /locus_tag="LR726_002096"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3085..3087,aa:Ser,seq:gct)
     gene            3160..3231
                     /locus_tag="LR726_002097"
     tRNA            3160..3231
                     /locus_tag="LR726_002097"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3193..3195,aa:Glu,seq:ttc)
     gene            3254..3343
                     /locus_tag="LR726_002098"
     tRNA            3254..3343
                     /locus_tag="LR726_002098"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3290..3292,aa:Ser,seq:tga)
     gene            3356..3429
                     /locus_tag="LR726_002099"
     tRNA            3356..3429
                     /locus_tag="LR726_002099"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3390..3392,aa:Met,seq:cat)
     gene            3448..3520
                     /locus_tag="LR726_002100"
     tRNA            3448..3520
                     /locus_tag="LR726_002100"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3481..3483,aa:Asp,seq:gtc)
     gene            3524..3596
                     /locus_tag="LR726_002101"
     tRNA            3524..3596
                     /locus_tag="LR726_002101"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3557..3559,aa:Phe,seq:gaa)
     gene            3601..3671
                     /locus_tag="LR726_002102"
     tRNA            3601..3671
                     /locus_tag="LR726_002102"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3633..3635,aa:Gly,seq:tcc)
     gene            3684..3757
                     /locus_tag="LR726_002103"
     tRNA            3684..3757
                     /locus_tag="LR726_002103"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3718..3720,aa:Ile,seq:gat)
     gene            3769..3840
                     /locus_tag="LR726_002104"
     tRNA            3769..3840
                     /locus_tag="LR726_002104"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3802..3804,aa:Glu,seq:ttc)
     regulatory      4029..4108
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            4106..5299
                     /locus_tag="LR726_002105"
     CDS             4106..5299
                     /locus_tag="LR726_002105"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL6217161.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            5429..6901
                     /locus_tag="LR726_002106"
     CDS             5429..6901
                     /locus_tag="LR726_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217162.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            6984..7772
                     /locus_tag="LR726_002107"
     CDS             6984..7772
                     /locus_tag="LR726_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL6217163.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7839..8507)
                     /locus_tag="LR726_002108"
     CDS             complement(7839..8507)
                     /locus_tag="LR726_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL6217164.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            8622..10334
                     /locus_tag="LR726_002109"
     CDS             8622..10334
                     /locus_tag="LR726_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217165.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            10337..12109
                     /locus_tag="LR726_002110"
     CDS             10337..12109
                     /locus_tag="LR726_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217166.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3482 a         2266 c         2927 g         3505 t
ORIGIN      
        1 aagaggagtt aaatgcttct tttcttaagg acaagctgtg atggggaggg aaataatagt
       61 accgaagttc ctgatgtcac actgccgaga aaagcttcta gtgagaaaac agcggcccgt
      121 accgcaaacc gacacaggta gtcgaggaga gaatcctaag gtgagcgaga gaactctcgt
      181 taaggaactc ggcaaaatga ccccgtaact tcgggagaag gggtgctgat catacgatca
      241 gccgcagtga ataggcccaa gcgactgttt atcaaaaaca caggtctctg caaaatcgta
      301 agatgaagta taggggctga cgcctgcccg gtgctggaag gttaagagga gtgcttagcg
      361 caagcgaagg tacgaattga agccccagta aacggcggcc gtaactataa cggtcctaag
      421 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatttgggca
      481 ctgtctcaac gagagactcg gtgaaatttt agtacctgtg aagatgcagg ttacccgcga
      541 caggacggaa agaccccatg gagctttact gtagtttgat attgagtgtc tgtaccgcat
      601 gtacaggata ggtaggagcc gtagaaatcg gaacgctagt ttcgatggag gcgctggtgg
      661 gatactaccc ctgcgttatg gccactctaa cccgcaccac taatcgtggt gggagacagt
      721 gtcagatggg cagtttgact ggggcggtcg cctcctaaaa ggtaacggag gcgcccaaag
      781 gttccctcag aatggttgga aatcattcga agagtgtaaa ggcagaaggg agcttgactg
      841 cgagaccaac aagtcgagca gggacgaaag tcgggcttag tgatccggtg gttccgcatg
      901 gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta tctcccccaa
      961 gagtccacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg
     1021 tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga
     1081 acgtcgtgag acagttcggt ccctatccgt cgcgggcgtt ggaaatttga gaggagctgt
     1141 ccttagtacg agaggaccgg gatggactta ccgctggtgt accagttgtt ctgccaaggg
     1201 cattgctggg tagctatgta gggaagggat aaacgctgaa agcatctaag tgtgaagccc
     1261 acctcaagat gagatttccc atttctttaa gaaagtaaga tccctgagag atgatcaggt
     1321 agataggtca ggagtggaag tacagtgatg tatggagcgg actgatacta atcgatcgag
     1381 gacttaacca ataggttaaa agaaaagaac tcggaaaaaa acttatgtga cttcaaatcc
     1441 agttttgagt gaacaaaatt tactcaataa aagataacac cacagtgtgg tggcgatagc
     1501 gagaaggata cacctgtaac catgccgaac acagaagtta agcttcttag cgccgattgt
     1561 agtgaggggt tgccccttgt gagagtagga cgtcgccacg cttaagttat ccatggaggt
     1621 ttagctcagc tgggagagca tctgccttac aagcagaggg tcagcggttc gatcccgtta
     1681 acctccatat gagccgttag ctcagttggt agagcatctg acttttaatc agagggtcac
     1741 aggttcgagc cctgtacggc tcatattttt gcgggtgtgg cggaattggc agacgcacta
     1801 gatttaggat ctagcgcctt acggcgtggg ggttcaagtc ccttcacccg cataatttaa
     1861 gttttacatg ccggcttagc tcagttggta gagcatctga tttgtaatca gagggtcgag
     1921 ggttcaagtc ctttagccgg catctttaaa cacgcggaaa tagctcagtg gtagagcacc
     1981 accttgccaa ggtgggggtc gcgggttcga accccgtttt ccgctttgcc aaagcaatcg
     2041 cgccggggtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggtg agtgatcacc
     2101 gtaccggttc gattccggtc ctcggcattt caaatatgcg cccatagctc aactggatag
     2161 agtgtttgac tacggatcaa aaggttaggg gttcgactcc tcttgggcgc gttaattaaa
     2221 cgggaagtag ctcagcttgg tagagcactt ggtttgggac caaggggtcg caggttcgaa
     2281 tcctgtcttc ccgataatta taaatttaaa cacggggcct tagctcagct gggagagcgc
     2341 ctgctttgca cgcaggaggt cagcggttcg atcccgctag gctccatttc atttaaataa
     2401 tgaatatctt tttcgcggtg tagctcagct ggctagagcg tccggttcat acccgggagg
     2461 tcgggggttc gatcccctcc gccgcgattt ttaagagcct ggacctttag ctcagttggt
     2521 tagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaagg tccattgtgg
     2581 cgttgtaagt ttcatatgga ggattaccca agtccggctg aagggaacgg tcttgaaaac
     2641 cgtcaggcgg gtaaaaccgt gcaagggttc gaatccctta tcctcctttt tcatattatc
     2701 gcgggatgga gcagtctggt agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat
     2761 cctgctcccg caattaaaaa ctaaaaacac tggttccgtg gtgtaggggt taacatgcct
     2821 gcctgtcacg caggagatcg cgggttcgat tcccgtcgga accgccatgc gggtgtagtt
     2881 tagtggtaaa accacagcct tccaagctgt tgtcgcgagt tcgattctcg tcacccgctt
     2941 ttagtttttt atgggcctat agctcagctg gttagagcgc acgcctgata agcgtgaggt
     3001 cgatggttcg agtccattta ggcccattag tacaaaatct tattggtttt ggggaagtac
     3061 tcaagtggct gaagaggcgc ccctgctaag ggtgtaggtc gggaaaccgg cgcgagggtt
     3121 caaatccctc cttctccgtt tcaatatttt gttttacctg gcccgttggt caagcggtta
     3181 agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc cgtacgggtc attaatttaa
     3241 tatagtttta catggaggat tacccaagtc cggctgaagg gaacggtctt gaaaaccgtc
     3301 aggcgggtaa aaccgtgcaa gggttcgaat cccttatcct cctttttcat attatcgcgg
     3361 gatggagcag tctggtagct cgtcgggctc ataacccgaa ggtcgtaggt tcaaatcctg
     3421 ctcccgcaat taaaaactaa aaacattggt tccgtggtgt aggggttaac atgcctgcct
     3481 gtcacgcagg agatcgcggg ttcgattccc gtcggaaccg tttggctcgg tagctcagtt
     3541 ggtagagcaa tggattgaag ctccatgtgt cggcagttcg attctgtccc gcgccattat
     3601 gcgggtgtag tttagtggta aaaccacagc cttccaagct gttgtcgcga gttcgattct
     3661 cgtcacccgc ttttagtttt tatgggccta tagctcagct ggttagagcg cacgcctgat
     3721 aagcgtgagg tcgatggttc gagtccattt aggcccattt attttattgg cccgttggtc
     3781 aagcggttaa gacaccgccc tttcacggcg gtaacacggg ttcgaatccc gtacgggtca
     3841 tggaagatac tttttgagta tctttttctt tttatctgaa agaatgattt taaaaagctt
     3901 agcagagtta tctttatgga tgattgtgct aagctttttt ggacagttct aatgtaaaat
     3961 atttttatat tttttaagca aaaaaatgaa aaaatacgag cagaaaactg ttttatctgc
     4021 tatacttggt tcgttgcaag ttcccgaaag gatccgcagg acggagatgc cttgtaaccg
     4081 aatttaacta gggggaatca ttataatggt agaaagacac ttatttacat cagaatctgt
     4141 ttctgaagga catccagata aaattgctga ccaaatcagt gatgcaatct tggatgcaat
     4201 tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt
     4261 tttagtattt ggagaaatat ctacgacggc atatgtagat attcaaaaag tcgtacggga
     4321 aacaataaaa gaaattggtt atacacgtgc aaaattcgga tttgatggag atacagcagc
     4381 tgtattggtt gcgattgatg aacaatctcc tgatattgct caaggagttg atgaagcact
     4441 tgagatccgg gatgaagata aaaaagacgt attagatgag attggtgctg gtgaccaagg
     4501 tttaatgttt gggtttgctg tagatgaaac accagaattg atgcctttac caatcgcttt
     4561 gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt
     4621 acgtccagat gcaaaatctc aagtaacggt tgaatatgat gatcaaggac aaccggaacg
     4681 cgtagataca atcgttattt caacacagca tgatgatgca gtagataatg aaacaattcg
     4741 tcatgatgtc atcgaaaaag tagtgaagga agttattcca gctgaattat tagatgatca
     4801 aacgaaatat tatatcaatc cgactggccg atttgtcatt ggtggtcctc aaggggatgc
     4861 cggattaaca ggaagaaaaa tcattgttga tacgtatggc ggttatgctc gtcatggtgg
     4921 cggtgctttt tctggtaaag atgcgacaaa agttgaccgt tctgccagct atgctgcacg
     4981 ttatattgct aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag tacagctagc
     5041 ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc aatactttcg gtactggaac
     5101 ggttccggaa gaagaactga ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg
     5161 aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg cttacggtca
     5221 ttttggtcgt acagatgtag atttgccttg ggaacatacg gacaaagtag atgcgttgaa
     5281 agcaagttta gcaaaataag ttatattcag ctggaacgat cgccaagtgg tggttgttcc
     5341 agcttttttt atggcagtta acctcttgaa aaagatagta attatttatc tatttttaga
     5401 tcctgtcata atgaaaggga ggaataagat gaaaaaagaa acaaatgtta ctttggtcac
     5461 agtgagtatt ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg taacaaccgc
     5521 tatgccaacg attgtcggtt cattacatgg aatggaaatc atgaactggg tattttcgat
     5581 ttatttgctg acaaacgcta tgcttactcc gatttacgga aaacttgcgg ataaagtggg
     5641 acggaaacca atctttattt ttgggacatt attgtttatt atcggttcgt tattttgcgg
     5701 tttatctcaa agcatgattg ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg
     5761 ggcaatgatg ccagtagctt tgacgatcat cggcgatctt tattctattg aaaagcgagc
     5821 aaaagtttta ggtttgaata gttcagcctg ggggattgcc agtgtttttg gaccgcttgc
     5881 gggaggattc atcgttgaaa cgatcagctg gcactggatc ttttttatca atgtgccgat
     5941 cggaattcta ttaattattt tgatttggct atatttaaat gaaccaaaaa tcatacatga
     6001 gtctaaacca atggatattt ttggtagttt tagtttgatg gccatgcttg tctcattgct
     6061 gttagggttc caattattag gagacgaagg aatgagtccg cttgtttctg ctttgctgct
     6121 aggtagtatc gtaatgttag gtatttttgt ttttgccgaa aaaagagcta aagatcctgt
     6181 catcatgatg catttattca aaaatccact atttgttgtg gtcaacttgg ctgcagcatt
     6241 ggctagtggt tttttgatgg gaatcgatgt ttatatccct atgtggatgc aaggtgtatt
     6301 agggctgaat gcagcaattg gtgggctggt attagcacca ttatcactag tctggatggt
     6361 cggttctttc cttgcaagca agtggatgac tcaaatgaaa actcattggg tcttgagaat
     6421 tggactacta atcgcagcta ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa
     6481 ctatttatgg ttttttgcta tatcaacggt catcggtata ggcttaggtg tgaccatgac
     6541 tacctcgact gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg tagcaacttc
     6601 ttttaatacg ttagttcgaa caattggtca aacagtcatg gtttctattt ttggtttatt
     6661 gataaatggg gtaactaaac gagaacttgc agcagcaaat ttaacaaatg atgcagatat
     6721 catgaataaa ctggtgaatc cacaaacggc taaatatatt gatgcggata ttttgttacc
     6781 attaagaaaa attctttatg atgggttaca ttccgtttat ctagttggct tgttactcgt
     6841 gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta
     6901 gtttcggtta agatatgtac gtagccgaat cattcaaatt taaagttatt caaaaataac
     6961 ttcttactaa ggaaggcgaa aatatggcat ttattcaagc aaatatttat tcgaatgtgt
     7021 tagaaatgga agtcaatcta aatgtgatac taccgcaaac aacaaaaaag gtgattggca
     7081 ccagttcaga gcaagagaca acggatatac ctgttttata tttacttcat ggaatgggtg
     7141 gcaatcatag tgtatgggaa agacgtacgt cgattgaacg ttatgtagca aactacggat
     7201 tagctgtcat catgccatct actgatcttg gctggtacac agatacagtt tatgacatga
     7261 agtattggac attcattgca gaagagctac ctgagatttg tcatgaatta ttcccgcaat
     7321 taacaagaaa aagagaaaaa acatatgccg ctggattatc gatgggcgga tatggcgcgt
     7381 taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg
     7441 tttctgtagg aaatcgaatg gatgatcttt tgatggttcg taaagcatct ttttgggaag
     7501 ggatctttgg accattggat caaatcgaag gttcaaaaaa tgatccgcaa tacttattga
     7561 aagaactagt aaaaagcgga aaaccagttc caagatttta tatggcttgt ggggaatctg
     7621 attttcttta tcaggcaaat caggaaatgg tatctgctat gcaggaacaa ggcttggcgg
     7681 tcacctttga acaaggacct ggcgatcata actggatctt ctgggaccaa tggattcaac
     7741 gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag atagctccta
     7801 tagagatcac aaacatagtg atctctatag gagctttttt actgctcgtt ttctcttctt
     7861 ttattttgtt ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt
     7921 tcattcaatt ctgcatggga ctgcatactt tctttaaatc cctctaattg attttcaact
     7981 acataattac gcataaatag aactgtttct ttcaaaaatt catttccgtc cataattttt
     8041 tgtcgaagct gctgttcatg ctgctgatcc attaactgtc caatcaataa attgtggaga
     8101 gaggcaatgg cctgatgact ttgggtgact gaaaatcctt cacgaacaag taattggatc
     8161 atttgctcca aatgttctaa tcgactcgga aaagcgggca cagtattgat ttctaattct
     8221 gccccacaag ggaaacgtgt ataaaggtca tagtaatttt ccatatactg cagcaactgt
     8281 tcattccaag gcaaagttgg atctggaagc gctaattctt cttccattgt ttcagccatt
     8341 ttttgcaaga gctgttgttt gtttttgaaa taccaatata aagcaggtgc ttgaacatga
     8401 attctgtttg cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc agccaggata
     8461 tcaaaggcgg cctgaatgat gattgcttga gaaagcttta tttccatttt atcaacctcg
     8521 tgtattgcat tatcttttta aacatggtaa attgttagtc ggtctaattt tacagtgtta
     8581 aattagtcct gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta atcttacgac
     8641 atgcgaaaaa atataaagta gctgttttta tttccttgct gtccgtagca gtcatggttg
     8701 cagcagcatt atggcaacct aagttactac agcaagtatt ggaagctatt attacagaag
     8761 ataatgatga aatgaaaaca attggtattt acctgatcag tctggctttg ctaggattag
     8821 ccgcaggtgt aacgaacacg attttctctg caaaagtagc tcaaggcgta agtgcagata
     8881 tccgagaaga agcatttcga aagatccaga cattttcatt tggaaatatc gaacagttct
     8941 ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa aatttagtga
     9001 tgatttcttt gcaatcactt tttcgaattc cattcttatt tattggtgcc ttcattttag
     9061 ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt acggtatttc
     9121 tgattacggc actttcattt acaaggatgg gcaagcactt catgatcatt caaaaactga
     9181 ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg gattcgtgta gtgaaatctt
     9241 ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac
     9301 acaacatcat agtcggtaca ttattttctg tgatgatccc gtccttcatg ctggctgcca
     9361 atttggctgt ggttggggcg atcttttttg tgagcgatct tgttaaagat gatcctacgt
     9421 taattggtgg aattgcttca ttcatgaact acttgatgca gattatgatg gcaatcatta
     9481 ttggcggaat gatgatgatg atgacttcaa gagcagccgt atcgctgaag cggattgcgg
     9541 aaattttaga tgctgaacca gatcttactt atttagacgt tccagaacaa gagttgacgg
     9601 gttctgtcac ttttgaccat gtttcttttc gttatcctgg tgaggataca gatacgctta
     9661 aagatatcag cttttctatc aaacctggag aaatggttgg tatcgtcggg gcgactggtg
     9721 ccgggaagtc aacactagca caattgatcc ctcgtctatt tgatccgaca gaaggcaaag
     9781 ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga caaacggttt
     9841 cctttgtttt gcaaaaagca attttattct caggaacgat tgctcaaaac ttgcgccaag
     9901 ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc ttctattgct caagcaaaag
     9961 aattcatcga aaagttatct gatcgttacg aagcaccaat agaagaacgt agcagtaact
    10021 tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt aatccaaaga
    10081 tcttgatcct agatgacagt acgagtgcac tggatgctcg ctctgaaaag ctggtccgag
    10141 aagcactcga tcgagatttg aagggaacaa cgacgattgt catcgcacaa aaaattgctt
    10201 ctgttgtcaa agcagaccgc atcttagtat tggatgaagg cagattagtc ggcgaaggaa
    10261 cccatgagga attggtagct ggaaaccgtg tctatcagga aatctttgaa actcaaaaag
    10321 gaacggagga gtaagtatga ctgatctaat aaaagcaagt aaatttttct atcactattt
    10381 gaagcggtac aaactctcct ttttctttat ctttatcaca gtgatcattg ctacctatct
    10441 acaagtaaag gcacctcaat acatcgggga agcttttcaa gaattagcta attacgtagg
    10501 aggcttgatg caaggcgtag acgataaaag caaatttgtt tctgtaatat ggaaactctt
    10561 gctgttttat gtactagcta gtgcagccaa ctttatctat agtattttgt tcacacaagt
    10621 agtagggaag tcaacgaacc gaatgcgtat cggtctattc aacaaattgg aaaaattgac
    10681 gatccgtttc tttgattctc atcaagacgg tgaaatttta agtcgtttta ccagtgactt
    10741 agataatatt caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt
    10801 acttgtcggg atcttgatca tgatgttccg ccaaaatgta gagttagctt gggctacaat
    10861 tgcttccact ccagtagccg ttttgatagc tgtcttgatt atccgcaaag cacggaaata
    10921 tgtggatctt cagcaagatg aagtggggaa actaaatggt tatatggatg aaaaaatcag
    10981 cggacaacgt gtcatcatca caaatggctt acaagaagaa acgattgaag gattcttaga
    11041 gcataatcaa agtgttcgcg aagcaacctt caaaggacaa gcttattctg gtctactatt
    11101 tccgatgatg caaggaatgt cactggtcaa tacagcgata gttatctttt tcggcggctg
    11161 gttagcatta aatggtgatt tggaacgttc ggtggcatta ggcttagttg tgacattcgt
    11221 ccagtattca caacaatatt accaaccact gatgcagatt tcatctggat acagcatgat
    11281 ccaattagcg attacagggg caagacgatt gaatgaaata tttgatgaaa aagatgaagt
    11341 caaaccagaa aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt
    11401 agattttggt tattctccag aaaaactagt cttgaaagat gtctcgatca atgtggacaa
    11461 aggagaaatg gttgctcttg ttggtccaac aggatcgggg aaaacgacga tcatgaatct
    11521 gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa tagatatccg
    11581 agattatgat cttgatgcat tacgtagaca tgtaggaatc gttttgcaag aatctgtatt
    11641 gttctccgga acgatcaaag acaatatcac attcggcaaa ccggacgctt cagatgaaga
    11701 agtcatcaat gcagcaaaac aagcgaatat tcatgacttc atcatgacgt tagaaaaagg
    11761 ttatgataca gagatatccg aagaaaataa cttattcagt acaggtcaaa agcagttgat
    11821 cagtattgct cgaacgatca tcacaaatcc atctttattg attttggatg aagcaacaag
    11881 taatgtagac acggtaacag aagcaaggat ccaaaaagca atggatgaag caatcaaagg
    11941 aagaaccagc tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt
    12001 tgtcttgcgt gatggggaag tcattgaaga aggcagtcat catgagttat tagcagaaaa
    12061 tggattttat gcggaacttt atcataatca atttgtattt gaaagttaaa ttaaaagaac
    12121 ctcgttataa cggggttctt tttatatatt ttatgttgtt tgtttttata aggggcagaa
//
LOCUS       DAFGFZ010000087        12074 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000087 DAFGFZ010000000
VERSION     DAFGFZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.089"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(21..578)
                     /gene="frr"
                     /locus_tag="LR726_002111"
     CDS             complement(21..578)
                     /gene="frr"
                     /locus_tag="LR726_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL6217167.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(581..1303)
                     /locus_tag="LR726_002112"
     CDS             complement(581..1303)
                     /locus_tag="LR726_002112"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL6217168.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1439..2320)
                     /locus_tag="LR726_002113"
     CDS             complement(1439..2320)
                     /locus_tag="LR726_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL6217169.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="LR726_002114"
     CDS             complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="LR726_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL6217170.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3560..4039
                     /locus_tag="LR726_002115"
     CDS             3560..4039
                     /locus_tag="LR726_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL6217171.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4256..5347
                     /gene="phnW"
                     /locus_tag="LR726_002116"
     CDS             4256..5347
                     /gene="phnW"
                     /locus_tag="LR726_002116"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL6217172.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5340..5468
                     /locus_tag="LR726_002117"
     CDS             5340..5468
                     /locus_tag="LR726_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL6217173.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5472..6017
                     /locus_tag="LR726_002118"
     CDS             5472..6017
                     /locus_tag="LR726_002118"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL6217174.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6471..8162)
                     /locus_tag="LR726_002119"
     CDS             complement(6471..8162)
                     /locus_tag="LR726_002119"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL6217175.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8582..9529)
                     /gene="arcC"
                     /locus_tag="LR726_002120"
     CDS             complement(8582..9529)
                     /gene="arcC"
                     /locus_tag="LR726_002120"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL6217176.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9644..10663)
                     /gene="argF"
                     /locus_tag="LR726_002121"
     CDS             complement(9644..10663)
                     /gene="argF"
                     /locus_tag="LR726_002121"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL6217177.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10754..11983)
                     /gene="arcA"
                     /locus_tag="LR726_002122"
     CDS             complement(10754..11983)
                     /gene="arcA"
                     /locus_tag="LR726_002122"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL6217178.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3292 a         2514 c         2190 g         4078 t
ORIGIN      
        1 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
       61 ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      121 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      181 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      241 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      301 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      361 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      421 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      481 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      541 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      601 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      661 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      721 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      781 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      841 cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      901 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
      961 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1021 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1081 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1141 cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1201 tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc
     1261 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1321 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1381 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1441 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1501 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1561 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1621 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1681 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1741 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1801 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1861 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1921 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     1981 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2041 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2101 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2161 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2221 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2281 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2341 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2401 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2461 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2521 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2581 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2641 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2701 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2761 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2821 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2881 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     2941 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3001 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3061 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3121 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3181 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3241 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3301 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3361 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3421 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3481 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3541 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3601 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3661 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3721 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3781 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3841 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3901 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     3961 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4021 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4081 tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt
     4141 attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4201 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4261 taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4321 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4381 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag
     4441 ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4501 ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4561 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4621 gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4681 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat
     4741 agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga
     4801 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4861 tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct
     4921 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     4981 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5041 aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat
     5101 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5161 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt
     5221 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5281 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5341 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5401 ctgtccacgc ttttagaaat gcttttctag aaaaaggaac ccaactgact gacaaagaaa
     5461 tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5521 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5581 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5641 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5701 cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt
     5761 attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt
     5821 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5881 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     5941 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6001 ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat
     6061 ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga
     6121 atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac
     6181 agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca
     6241 agcttttgaa caccgtttat tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6301 gtccccttat ttgcaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6361 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6421 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6481 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6541 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6601 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6661 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6721 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6781 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6841 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6901 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     6961 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7021 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7081 ggaatatggt gcatgtcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7141 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7201 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7261 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7321 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7381 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7441 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctctttatcg
     7501 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7561 gctacttcat ggaattggac gtataaacga agcaattcat tgattggttc tgctttaaca
     7621 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7681 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7741 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7801 gaagacatgt cgattggtac tgttccttga ttcccgatgt tgctatcccc ataatgttct
     7861 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7921 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     7981 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8041 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc
     8101 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8161 atctttattc ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8221 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8281 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8341 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8401 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8461 tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa aaaaatccag atggcctaaa
     8521 ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa
     8581 attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta
     8641 aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta
     8701 ctttcggaag catgcttcct ggagcgaatt gcttttcatc aatgtattgt ttcatttctg
     8761 aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt
     8821 ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag
     8881 caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag
     8941 gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga
     9001 cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc
     9061 cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt
     9121 ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca
     9181 caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt
     9241 cgcccatggc gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat
     9301 ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac
     9361 cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca
     9421 ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag
     9481 acaagatggc attgccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc
     9541 tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc
     9601 ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa
     9661 acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt
     9721 ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga
     9781 tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca
     9841 agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga
     9901 tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata
     9961 cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa
    10021 tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt
    10081 caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga
    10141 ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt
    10201 ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc
    10261 attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca
    10321 ttttttgact gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg
    10381 tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat
    10441 gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt
    10501 caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca
    10561 agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt
    10621 tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac attctccttt
    10681 agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag taagactgcc
    10741 gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac
    10801 caccacgacc tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac
    10861 gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg
    10921 ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac
    10981 cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt
    11041 tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct
    11101 taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa
    11161 atacggtatc caaatgcatc attgcacggt tgttcggaat tttgattgcc aacacttttt
    11221 caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag
    11281 ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt
    11341 cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg
    11401 gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca
    11461 tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt
    11521 ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatca tatagactgc
    11581 tagaacgtac ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt
    11641 gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat
    11701 gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt
    11761 ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat gtcttcgcaa
    11821 agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat
    11881 ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt aaaactgtct
    11941 ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc
    12001 tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat
    12061 tcaaaatgag acat
//
LOCUS       DAFGFZ010000088        11967 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000088 DAFGFZ010000000
VERSION     DAFGFZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.090"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            187..2022
                     /gene="tdc"
                     /locus_tag="LR726_002123"
     CDS             187..2022
                     /gene="tdc"
                     /locus_tag="LR726_002123"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL6217179.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2327..3805
                     /locus_tag="LR726_002124"
     CDS             2327..3805
                     /locus_tag="LR726_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6217180.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3771..5102
                     /locus_tag="LR726_002125"
     CDS             3771..5102
                     /locus_tag="LR726_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6217181.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5216..7768
                     /locus_tag="LR726_002126"
     CDS             5216..7768
                     /locus_tag="LR726_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL6217182.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7775..9214)
                     /locus_tag="LR726_002127"
     CDS             complement(7775..9214)
                     /locus_tag="LR726_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217183.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9560..9922
                     /locus_tag="LR726_002128"
     CDS             9560..9922
                     /locus_tag="LR726_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL6217184.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10109..10966)
                     /locus_tag="LR726_002129"
     CDS             complement(10109..10966)
                     /locus_tag="LR726_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL6217185.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11086..>11967
                     /locus_tag="LR726_002130"
     CDS             11086..>11967
                     /locus_tag="LR726_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL6217186.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDD"
BASE COUNT         3776 a         1991 c         2556 g         3644 t
ORIGIN      
        1 attgttcgct tttttgcgtt gaattatcag aaaaatccga ctttatttat ttttgtttgt
       61 aaaaatctac gtggatttga tgttataaaa aaaaaattct catttttggc gctatagtga
      121 agaggtgtta cagataaaca catgtatctg caagacattg accaacaaaa tatggaggga
      181 agaaaaatga aagatatgga tatcaaggcc gtctttatag gcgacaaagc tgaaaatgga
      241 ccagtttata aaatgttgtt gaacaagatg gtagatgaac atcttggatg gagagaaaat
      301 tatattcctt cagatatgcc tgcaattagc gaaggagata agctcactcc agattatcta
      361 gccacccgcg accatatgat agaggtcttg gatgaagtca gtcagcgatt acgcgcagga
      421 tcgatcccat ggcattcagc aggccgttac tggggacaaa tgaatgctga aacattgatg
      481 cctgctttat tagcttacaa ctatgcgatg ctgtggaacc caaataatgt tgcattggaa
      541 tcttctatgg caacttcgca aatggaagca gaagtaggac aggattttgc ttcattattc
      601 aatatgacag acggatgggg acacattgca gcagatggtt ctattgctaa tcttgaagga
      661 ttatggtacg cccgttgcat caaatcgatt cctttagctg tcaaggaagt gctgcctgaa
      721 aaagtgaaaa aaatgtctga gtgggaactt ttgaatttat ctgttgaaga aattttggaa
      781 atgaccgaaa gctttacgga cgaagaaatg gatgaggtca aagcagcttc ttcccgtagc
      841 ggaaaaaata tccaaagatt agggaaatgg ctggtccctc aaacaaaaca ctattcttgg
      901 atgaaggcgt tagatatttg cggagtcggc ttagatcaga tggttgctat cccagttcaa
      961 gaggattatc gaatggatat taatgctttg gaaaaaacaa ttcgtgaatt agctggacaa
     1021 aaaatcccaa ttctaggtgt ggttgctgtc gtaggaacga cagaagaagg tcaagtagac
     1081 agtgtagata aaatcgtcca attaagagaa aggttaaaag atgaagggat ctatttctat
     1141 ttacatgttg atgccgcata tggcggctat gcgcgttcac tgtttctaaa cgaagcagga
     1201 gaatttgtgc cgtatgcttc cttagctgaa ttctttgaag aacatcatgt tttccaccac
     1261 tgcgtaacga ttgacaaaga ggtatatgaa gggttcagag ctatttcaga agcggattct
     1321 gtcacgatag atcctcataa gatgggctat gtcccttatg ctgctggagg aatcgtaatc
     1381 aaacataaaa atatgagaaa tatcatttct tatttcgcgc catatgtgtt tgaaaaatca
     1441 gtaaaggctc cagatatgtt aggagcttat attttggaag gatcaaaagc tggagcaacc
     1501 gccgccgcag tctggacagc acatcgtgta ttgccattga atgtgaccgg atatggtcaa
     1561 ttgattgggg cttcaatcga ggcagctcaa agattcagag aatttctcga tcatctgact
     1621 tttactgtta aaggaaaaac aatcgaagtc tatccattga atcatcctga ttttaatatg
     1681 gtcaattggg tattcaaaga acaaggctgt acagatttga acgctatcaa tgaattaaat
     1741 gaaaaaatgt tcgatcggtc ttcttatatg gatggcgatg tttatggtga acggtttatt
     1801 acttctcata ctacatttac acaagaagat tacggtgatt ctccgattcg ttttgttgaa
     1861 agaatgggtc taacgaaaga agaatggaaa aaagaacaga aaatcactct gctacgtgcg
     1921 gctatcatga ctccttattt gaatgatgat cgaattttca atttttatac aaaaaaaatc
     1981 gccaaagcaa tggaaaagaa actaaacgaa atcatccaat agaactgatt tttgtgtgaa
     2041 agaataacaa gagacatatg gagaacagac aattatttgc ttcatcaaaa caattgtatt
     2101 tccccctgtt tttcatatat tcctcgccaa gatattcttt tttataaaac gttctataag
     2161 ttggatcgtt cgtttcatgt atcaaaccca gttcactaag atcgttcaat ggatgtttcg
     2221 gtgatactgg gttttgtact gaatcgatga aaccttgtca tttctgttct tgaacaaaaa
     2281 taaaagaaaa caaaaacata tgtcgctatt gaaagaagga ataaagatga aagatactgg
     2341 ttcatcaaag caaatcacga ccatgacctt tatcggaatg acctgcgccc ttgtagcaag
     2401 tgtacgaaat attccagatg tagcagcaac gagctggacg atgatttttt acatgctggt
     2461 tgcagtatta ttgtacgctt ttccaatctc attgatctct ggtgaatttg ctggaatgtt
     2521 tccccaaaaa ggtggtccag aactttgggt atcgaatgct ctaggaaaaa aatgggggtt
     2581 cgttgtctca tggctgttat gggtccaaat gtttcctggg atggtgatgg tcgcttcagc
     2641 actcgctccg ttgtttggta atattatcga taatgtgccg cttggtttga acagcaaatt
     2701 tacattagtt gtcattttag tcgtttattg gatcatcact tttttgaatt tgaaatttga
     2761 tatggcaaaa atcggcggaa aagtcggtgt ttggttagga ttgtacgtgc ctttgacgat
     2821 gatgctctta ttagggtttg ctgcgtggat caaagttggg atcgtaccaa ccggaacatt
     2881 aggcagtttt tcatgggaca aattaatacc agatactacg actgcatcct catttgttta
     2941 ttttgcgccg atcatgttta tttttactgg tatcgaaatg tcctctgtgt atatcactcg
     3001 attggaaaat ccagttaaaa cgtatattcg cggagtattt gccgcgttaa tttttatatt
     3061 tttcgtcaat attttgaatg ctttagttgt agcaaatgtc gtaccaaaag gacaaatgga
     3121 attgaataat atcgcgcaat ctatttcgat ttattgccaa attcttggtt tgccacatct
     3181 tattgtgaat ctgtttagtt tacttgtctt cattggtgta gcagtccaat tatctgcttg
     3241 ggcctcagga ccagcaaaga cagtgacaga aagtgctaga aaaggtgcgt atccaccaaa
     3301 attcaatttt tggaagacga atcaatttga cgtatccaaa tccgttattt tgactcagtc
     3361 tgtcattatc tcaatctttg cactttttta tttgctgatt cctggcgtca atcaagcatt
     3421 tttgatgctc gtaaattcaa ctactgttat ctactgtatc gtctatgtca tcatggggat
     3481 cgcagttttg agattgcgtt atacacatgc aaagttgaat cgaccattta gaattggaaa
     3541 aaaagagaaa aagagcaatc ttggagtttg gactgtcgtt attgttttat ttgcggcaat
     3601 cggattttct gtcggactga cgatgaaagc tggaacatgg atcaatctaa ttgcagtcac
     3661 agccatcagt gtcattttgt ttgtagtacc actctgtatt gaaaaaataa agaaaccttc
     3721 ttgggaacag gaagtcgcca tgctgcttga aaaagaaaaa ggtgaacttg atggaaaaac
     3781 cggcaatgaa acggactcta actaatcgaa atatccagtt aatcgcatta ggtggcgcaa
     3841 ttggaacagg attattttta ggttcagcaa aggcaattgc atcagcaggg ccagctgtta
     3901 ttctcgttta tttgatatgc ggtttggcta tttatggttt gatgcgggcc atgggtgatt
     3961 tatttttagc aaatagtaca tttaactcta tcagtgattt cgttgactat tatttaggca
     4021 aaaagtgggc attttttgtc aattggactt attggctttg ctggcttgga gctggacttg
     4081 cggaattaac agcaatagga ttgtatatcc atttctggtt tgtacatgta ccagctttga
     4141 taagcggagg tagcgcacta atgatcttaa tggcagtcaa ccttgttgca gtcaagtggt
     4201 ttggtgaatt ggaatctttt ttctcaatca tcaagatctt ggggattttc ctttttatct
     4261 tattgggtat aggattgagt gtctcagatt ttcataccca gtctatcgga atcaaagaaa
     4321 attttggaca gatcgatgaa ttactaccgt ttggctttgc tggaatggta gtctcatttc
     4381 ctatggtttt attcagtttt gctggaattg aaatgatcgg cttaaccgtt ggcgagacaa
     4441 gtgatccgca aaagaatttg aaaaaagcaa tcaactgtct gccttggcgt attctctttt
     4501 tctatatagg aacgattttg tcgattttat ttgttgttcc atggcattgt ttagatttaa
     4561 aagaaagtcc tttagtgact atgctgcaag tgatccattg ccaaggcgga gcagcaattg
     4621 tcaatctaat gattttggtt gcttctttgt cctcagcaaa tagtgcagtc tacagtacta
     4681 gcaggatttt atatcaaaca gcaaaagaaa aaagtttgcc ccattggttt tgtgcgcttt
     4741 ctaagaaagg cgtaccttta cacggtattt taattacagg aatgttattt tttgccggtc
     4801 tttgtctaca tttttttgta gctgatagtc gattgttttt tcagcttttg gcaggcatga
     4861 caacgagttg ttttctattt gtgtggtctt cgatcttgtg tgcacatgtt caatttatta
     4921 aagaaaagaa acggaacact acacaaagat ataaagatct tgctttgttg gtgtttttta
     4981 cttcaattgc tttcagcttg ttattcgaaa gcacgacacg ctttattcct tttttattgg
     5041 ttatctggtt tgtattctta aattttgtat ataaaagaat aagcaataga aatatcagtt
     5101 aggcccagat acattttgca aaacgaatta tttcagaccc ccctgaaata atcgatacgc
     5161 ctgaaaaaac ttccctgcgt ataataacag tatcaattaa acaggagggg ataggatgaa
     5221 ctggtacaga ttgacggtag aagatgtatt gaaaaactat caaacatcaa cagaaggctt
     5281 gtcgcaagaa gaacgacaga aacgcctgac tgatcaagga tataatgaaa taaaagcaag
     5341 gcagcagaca aaacgatgga aaaaaatagc aaagcatttt actgatctgc tgatgatcgt
     5401 gttgatcatt gcctcttttt tgaaatttgc ttctagtgaa tatattgaag gaagtattat
     5461 tttattcgta gtgatcgtca atggactcgt tagttactgg caagaaagaa aagcggaaga
     5521 atcattgaat ggtttgaagc agctgatggg acaagaagcg atagtactat caaacgggat
     5581 gcaagaaaaa atccctgcga aagaactggt aaaaggagat gtcattgtct tacgtgccgg
     5641 agatgtcgtt ccagcagata tccgattaat cgaagcacat gatttattag tggaagaatc
     5701 gattttgacg ggagaatcag aagcatctga aaaaagtcat gccgttttga cagaagaaga
     5761 atcgatcggc gaccagaaaa atatgggttt ttcaggaacg cttgtacaat ctggttctgc
     5821 agtcggtgtc gttgtagaaa caggaaacac gacagaaatc ggtaaaatca atcaagcatt
     5881 gcaatcggtc gaacaacaaa cgacgcctct gattaaaaaa atcaaccgat taaacaaaca
     5941 aatttttcaa ggcattcttt gtttgagcct attcttagtt atttttacga catttcgata
     6001 tggtatggat tggcatatcc ttctttcggc tacgattgct ttggtggtgt caatggtacc
     6061 cgaaggattg ccagctgttt tgaccatgat tctatccgtt ggagtacatg aaatggcaaa
     6121 agaaaaggcg atcatcaaag gacttccttc ggtagaaaca ttaggttcta tgacagtgat
     6181 ctgttcagat aagacaggaa cattgacaaa aaatgaaatg acagttgtgg atgtagctgc
     6241 aaaagaagaa gcctgcgtgt tgtccattat gaaaaactgt caggaattaa aaacaaaaga
     6301 agaacagaaa acagaaaatt taagtggcaa tccgacagaa gttgcgctga ttcgacacac
     6361 agaaacagcc aatctacctt tgaaaaaaat agaagcaaag attccgttca gttctgaata
     6421 caaatatatg gcaacgatgc atgcggagga aaaaggagcg gtgatttacg tcaaaggtgc
     6481 accagaagta ttatttgcta aatcaactct ttcttctgca gaacaagaag catggagtca
     6541 gacagctgca gaatttgcga gaaaaggtca gcgtgtttta ggatttgctt ataaaaaagt
     6601 agattcgaaa caggaactca cgcatgagac tttaacccaa ttaacttttg cagggatcgc
     6661 cggattgatc gatccgccaa aagaaagtgc agtcaaagct gtaaaggaat gccaacaagc
     6721 agggatctca gtgaaaatga ttacagggga tcacaaagac acagcaaaag cgattgcgga
     6781 acaaattggc ttaaaacata ctgccaaagt tttagaaggg atcgatttgg atcttatgtc
     6841 tgatgaagag ttgattcagc aagtgccaat agtcgatgtc tttgctcgaa cgacaccaga
     6901 acataaactg cgaattgtta aagctttgca gaaaaatgga gaaatcgtcg gaatgaccgg
     6961 agacggcgta aatgatgcac ctgctttgaa aagatccgat gtaggaattg ctatgggaat
     7021 caaaggtagt gaagtaagca aacaggcagc tgatatggtt ttaggagatg ataatttcca
     7081 tacgattgca aaagccgtaa aagagggccg gcggattttg gataatttgc aaaaaaccat
     7141 caatttcttc ttgccaactg cattagctca aggattgatc ttgatttggg cacttatgct
     7201 gaatcgcccg ctacctttgt ccccagtaca gattttatgg gtcaatatgg taacgacaat
     7261 cactttatct tatgcactag ggttcgagtc gccagatcca gaaatcatga agcgcccacc
     7321 aagagatcca aagcaaggaa tcctatctgg ttatcatttg tttcggatca tctatgtctc
     7381 cctactgatc atggttcctg cctatttatt ggctatgcag tttgaagggc aaagcttaca
     7441 gcaaaccatt cttctgcaaa atatcgtctt agctcaagct gtttacatga tcaattgcag
     7501 agagttgtcc aagccgtcat tgaaccaagg tcttttgcaa aacaaagcgc tttttctttc
     7561 attaggtatc cttgctttat tgcaagggat ggttattttt ctaccagtcg gccagcagct
     7621 gatcggtaca gtaagtttaa cgatgacaca acagttattg attggagcaa atgtgattct
     7681 tctattctta gttgtagaaa tagaaaaagg gatcatgaac aaactgtttc gcaaaaaaga
     7741 aaaaacagct agagaataca gtcgctagcc gttactataa aaaagttttg aaagtgagtt
     7801 cagtcaaacg attgagctca tttttcgttg gaatagaaga aatgtataca atagggatat
     7861 ctagagaatc tagagggaaa ttggaaatca agatatctgt tttttcaaaa gaaatagtgg
     7921 aaagatcggt tagttcgata ggtaagaagc agacacgttt tcctaagtaa tcttttaatt
     7981 cctgctgcaa gaacgccttc catgaaggtt ctccttggaa aataaaatag acattgaaca
     8041 aattcggttt gatagaaaaa atcgtttgtt ggatcagtag agacaagagt tggagaaagt
     8101 aatttggttc attgatttct atttcttttt ttagatcgtt cagtaaattc tctttctgtt
     8161 gatacaagtg tgcatattgc tgatcatatg tttctaaaaa acgatggtat tgacggtcca
     8221 atgcttcaat caatatcggc gattcatgat attttaatat gaggagagcc aagttatcag
     8281 tcagagatgt tttttttagt tgatcaagag acagctctga agctagttcc tctacaaacg
     8341 tttcgaggac ctctaaaaat ggagaatgat gcaggcagcg agcggtttct tgtccatagt
     8401 tattgttgta attccaactt tccagaaagc agaaagaagc aaaagaaagc tcagtgtctt
     8461 taagataaat accttccgtt aaatacaatt ttttcaatgc agagtcatac agggaggaga
     8521 gtgtgctgtt ggtatggata gaagaatcta atcggcaatt cgcttttata cggatcgtat
     8581 tgataaagaa ccaacatgtt ccgaaaatct cttctgtttc aaccgctagt tcagtttgac
     8641 tgaggtttct taaaaattga aaataatggg attcatggat cgaataatcc tcgaaaaaat
     8701 agttgttatg agtgaaagga atcagcaatc gatgataaaa agtacgaatc gcacgctctt
     8761 ttccaagaat acaagctgta tatttcgtca aatcgatctg caagtcgtaa cttgctaaac
     8821 gctgattgat tttagtcaca tgtcttttca atgtcccata agaaagccca tggtcggcta
     8881 gaaaatcagg gacagcaact ttttttgtaa aaagcagatt tttcagcagt tcgacactga
     8941 ctgattgttt gaaaaaaatc tcccaaaggt catttgttaa taaattttgc tggttttgca
     9001 aactgattcc tgcttgatcg ctgatgatgt cccagccttc gggcagctga gagcgaatag
     9061 attgaaggtc agaaaaaatc gttcgttcgg ttgtccctat gatttcagct aattcattta
     9121 cggttatctt tgtatgattc agcaattgtt ctactaaaga aacatgacgt cgaatatctt
     9181 tttcggtaat gatttttttc attagtgaat acatcaatca gactcccttc tattctaaga
     9241 atacggagaa aataaacggt ggtcaagttt caactgattt ttcaatcatt gtttattttt
     9301 ctggagcatt tactgatgat aagaatttca tcatgaactt gaaaaatgat accactaaaa
     9361 tcaaaaaata ggtgggcaaa caataataaa cattttaata gtagatggta gcaagaccaa
     9421 cacaggcgga ttgaacattt aagagttgat aaaaaagaaa aatatcgaag tatttgatta
     9481 gcaactactt gtattcattt ttctattctt acgtggtatc tttgaataaa gcgaatcaat
     9541 aagaaagaga tggaagcaga tgctaacgaa aaaagaacta atcgatcaca tcaatgaaac
     9601 atacaatttg tctccagaat atatttttaa aaaatttcct gattattgcg tattcagaca
     9661 tcaaaaaagc aaaaaatggt ttggggtcct gatgtctgtt ccagaagaaa aaatctatga
     9721 tgatgcaagt gaccatagga tcatcaatat tcttgatttg aaggtcgatc ctgaacttgg
     9781 cggaattttg cgaggaaaag aggggtattt tccagcctat cacatgaata aagaacattg
     9841 gatctctatt gatctacgta aaatagaaaa gctagatgaa ctagcagatt tgattgatgg
     9901 aagttttaaa tcaacgcaat gaatgtctag gacaatagag aagaggttgt gacagaaatg
     9961 tttagcttca agaaataaga aggaatttac gaaaattgct tctcaaattt ttgggaaatt
    10021 tcggcttatt ttctaagaaa ttacttctgg aacaccgttt gttcgaaaaa agagctgcga
    10081 caagaatttt gtcccagctc tttttttttt attttcctaa ataaaattcc cgcatcgttt
    10141 ttgcaatcat tttgatcaaa ggatgatctt ctgtgaagct agaagataaa taaattgttc
    10201 gtgtcaaggg caaatctttg atttcccgca agacgaaagg atcattttta tcgtaagacc
    10261 aagaaacttt tgggaaaaag ctaacagcta atccttgctt gacaatagaa cgtaatgtcg
    10321 caggatcatc tgtcgtcgaa ctgatcgtca attcgatact tttcgcttca agtgctgcat
    10381 ccagtgtatg acggaaggcg ttgctctttg acagcatcaa gatgggggtg cgttggatat
    10441 cttctaattg cagatgttct tttttagcaa gaggatgttt cttaggaatg gcgacataaa
    10501 caggttcttt catgatagga atattggtca tgccaggctt catctccgaa gtgatgtaca
    10561 gtacaggatc ggtaatatca tctggtaaat gatgttgcat gatcgttaat tggatatcag
    10621 gatgcttttc cataaaaatt cggaccaagt tcggaataga aacagaggct acctctaaat
    10681 acaatatgac ggaagtaacg atcttctcat tgaccgaaga caataatttg attccttctt
    10741 ctaatgtgtc taatgtcttg actgcatatg gataaaattc ttctgcataa acggtcgtct
    10801 caatgtttct gccgctatgg taaaacaaag ggacacctaa ttcgttttcc aaatgtttga
    10861 tcgcacggct caaggcaggt tgagaaataa ataattcttt ggctgcttgg gtaacatttt
    10921 ttgtttcgaa tactttttta aaatactgca gatgaagcaa tttcataatt atccatccca
    10981 tccatacctt tttgttatgg ttttttcttt ttttggcatt ttttattata gcagaaattg
    11041 actaagatag cagtggaaaa agtaagtgct gaggaggcga ggctcatgag tacaggtttc
    11101 ttatttcctg gacaaggcag tcagaagtta gggatgttag cggatatccc tgaaatgtac
    11161 atggaacgtt tagaaaatgt gacggggtat cggttagttg ataaagaaga aaactatcaa
    11221 gatacggttt ttatccagct agcattactg acaaaagcag tattttattt agatgaaatg
    11281 gaaaaaaacg gtattcagcc tgatattgca gcaggacatt cgattggtgc tttttgtgca
    11341 gctgttgctt gtcgatcttt gagatttgag aatgcagcgg aactagtcta tcaccgcgca
    11401 gtcttaatga aagaagctta tccaagcggt tatgcaatgg gcgtagtagt aggtgtgacg
    11461 agaagcgaag ctgaggaaat tgtagcaaaa acatttgatc ctgaccatcc agtttatctg
    11521 tcaaatgaaa attgtccgat gcaacatacg ttatctggga gtatcgaagg tttatccgtt
    11581 acgttggcag aagccaaacg gcaccaagcg caaacagcga agctgctgaa agtccctgtg
    11641 ccatctcact gtctcttgat gaatgatacg gtcaagaaat tcaagccgta tatgcagtct
    11701 gtccatctat ctgatgctag atgtttgtat ataaaaaata cggatggaag agccactcag
    11761 gacgcagaag agattcgtaa agatttgttg gaaaacctag ctttccctgt gcagtggaac
    11821 caaatgatgg acttggcaaa agaattagga atggatctga cgattgaatt tccccccggc
    11881 aatactttga caaagctgat tcatacaaaa tttggtgtag ataaagggat ccgtacgatc
    11941 aatctagacc aacatggtat tgatgac
//
LOCUS       DAFGFZ010000089        11925 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000089 DAFGFZ010000000
VERSION     DAFGFZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.091"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(1..330)
                     /locus_tag="LR726_002131"
     CDS             complement(1..330)
                     /locus_tag="LR726_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL6217187.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="LR726_002132"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="LR726_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL6217188.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="LR726_002133"
     CDS             1251..2264
                     /locus_tag="LR726_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL6217189.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="LR726_002134"
     CDS             2264..3541
                     /locus_tag="LR726_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL6217190.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..6306
                     /locus_tag="LR726_002135"
     CDS             3604..6306
                     /locus_tag="LR726_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL6217191.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6458..6775
                     /locus_tag="LR726_002136"
     CDS             6458..6775
                     /locus_tag="LR726_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217192.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6805..7125
                     /locus_tag="LR726_002137"
     CDS             6805..7125
                     /locus_tag="LR726_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL6217193.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7233..8693
                     /locus_tag="LR726_002138"
     CDS             7233..8693
                     /locus_tag="LR726_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217194.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8761..8982
                     /locus_tag="LR726_002139"
     CDS             8761..8982
                     /locus_tag="LR726_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217195.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9013..9195
                     /locus_tag="LR726_002140"
     CDS             9013..9195
                     /locus_tag="LR726_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL6217196.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9195..9608
                     /locus_tag="LR726_002141"
     CDS             9195..9608
                     /locus_tag="LR726_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL6217197.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9731..10912
                     /locus_tag="LR726_002142"
     CDS             9731..10912
                     /locus_tag="LR726_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217198.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10983..11147)
                     /locus_tag="LR726_002143"
     CDS             complement(10983..11147)
                     /locus_tag="LR726_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217199.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11301..11374
                     /locus_tag="LR726_002144"
     tRNA            11301..11374
                     /locus_tag="LR726_002144"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11335..11337,aa:Arg,seq:cct)
     gene            complement(11443..11718)
                     /locus_tag="LR726_002145"
     CDS             complement(11443..11718)
                     /locus_tag="LR726_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6217200.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         3927 a         2012 c         2497 g         3489 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
     5341 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
     5401 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
     5461 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
     5521 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
     5581 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
     5641 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
     5701 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
     5761 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
     5821 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
     5881 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
     5941 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
     6001 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
     6061 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
     6121 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
     6181 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
     6241 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
     6301 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
     6361 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
     6421 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
     6481 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
     6541 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
     6601 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
     6661 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
     6721 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
     6781 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
     6841 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
     6901 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
     6961 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
     7021 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
     7081 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
     7141 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
     7201 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
     7261 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
     7321 atgcttttat cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
     7381 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
     7441 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
     7501 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
     7561 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
     7621 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
     7681 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
     7741 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
     7801 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
     7861 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
     7921 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
     7981 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
     8041 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
     8101 gaccaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
     8161 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
     8221 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
     8281 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
     8341 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
     8401 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
     8461 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
     8521 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
     8581 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
     8641 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
     8701 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttctagg aggaactgaa
     8761 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
     8821 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
     8881 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
     8941 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
     9001 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
     9061 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
     9121 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
     9181 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
     9241 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
     9301 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
     9361 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
     9421 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
     9481 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
     9541 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
     9601 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
     9661 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
     9721 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
     9781 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
     9841 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
     9901 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
     9961 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    10021 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    10081 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    10141 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    10201 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    10261 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    10321 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    10381 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    10441 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    10501 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    10561 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    10621 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    10681 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    10741 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    10801 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    10861 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    10921 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    10981 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    11041 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    11101 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    11161 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    11221 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    11281 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    11341 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    11401 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    11461 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    11521 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    11581 aagtatattt actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat
    11641 aaacagtcga agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac
    11701 ggtgtagttg attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac
    11761 cagtcctatc caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta
    11821 cgtaaagcca atgggaatga actggagatg actttttatt atttttagta atagctgatg
    11881 ggttaatact tacaactttc cttttcaaag taccatataa aaaaa
//
LOCUS       DAFGFZ010000090        11851 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000090 DAFGFZ010000000
VERSION     DAFGFZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.092"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            215..415
                     /locus_tag="LR726_002146"
                     /pseudo
     CDS             215..415
                     /locus_tag="LR726_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1127..1732)
                     /locus_tag="LR726_002147"
     CDS             complement(1127..1732)
                     /locus_tag="LR726_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217201.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            1914..2249
                     /locus_tag="LR726_002148"
                     /pseudo
     CDS             1914..2249
                     /locus_tag="LR726_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2414..2641
                     /locus_tag="LR726_002149"
                     /pseudo
     CDS             2414..2641
                     /locus_tag="LR726_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2767..3738
                     /locus_tag="LR726_002150"
     CDS             2767..3738
                     /locus_tag="LR726_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217202.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            3878..5191
                     /locus_tag="LR726_002151"
     CDS             3878..5191
                     /locus_tag="LR726_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL6217203.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5234..6100
                     /locus_tag="LR726_002152"
     CDS             5234..6100
                     /locus_tag="LR726_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6217204.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6097..6945
                     /locus_tag="LR726_002153"
     CDS             6097..6945
                     /locus_tag="LR726_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6217205.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            6959..8431
                     /locus_tag="LR726_002154"
     CDS             6959..8431
                     /locus_tag="LR726_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBL6217206.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8448..9320
                     /locus_tag="LR726_002155"
     CDS             8448..9320
                     /locus_tag="LR726_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL6217207.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9556..10938)
                     /locus_tag="LR726_002156"
                     /pseudo
     CDS             complement(9556..10938)
                     /locus_tag="LR726_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11400..11681
                     /locus_tag="LR726_002157"
                     /pseudo
     CDS             11400..11681
                     /locus_tag="LR726_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            11755..>11851
                     /locus_tag="LR726_002158"
     CDS             11755..>11851
                     /locus_tag="LR726_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL6217208.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQ"
BASE COUNT         3674 a         2036 c         2390 g         3751 t
ORIGIN      
        1 gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag aatgaaacac
       61 attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta agttttttca
      121 tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc ccgttattga
      181 agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa tatttgagaa
      241 acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga tcacctccta
      301 ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt gccagttaga
      361 tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga attgatcaga
      421 aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg ctcagggcaa
      481 ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc actttagaat
      541 acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat ttaaacaagt
      601 tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca aggcaataga
      661 tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga ataaccttct
      721 gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta ttttattggt
      781 tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa cgattagttt
      841 aactgtggga agctccagac atacgattcg agaagttact tctcgagcag acaggaaggg
      901 ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc tttttccgga
      961 aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc ataaagaact
     1021 aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac atggaacata
     1081 gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac aaatcattcg
     1141 ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca atttctgcaa
     1201 ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt ttttgcggat
     1261 ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct tttttggcca
     1321 ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt gcaatataac
     1381 ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt ccagtattaa
     1441 aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc ataaaggtaa
     1501 ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca acaatgacac
     1561 aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg atatctttac
     1621 cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt gccaattgtc
     1681 gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc ataaaaactc
     1741 cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact gttatcatag
     1801 catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt tgaatgaatg
     1861 aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc tcggaacagt
     1921 taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt agaaaaggca
     1981 aactttatca aacttattac gacggtatgg aggaacagct caacgcatta ggattagtaa
     2041 ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa caaattaaaa
     2101 cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta attagtgatc
     2161 acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg aatggaatac
     2221 tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc taccgtgcat
     2281 tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac acaagaatac
     2341 taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt cgtttctatg
     2401 ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca ttgacgactt
     2461 agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg gtcctgttta
     2521 ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg aaataaaatt
     2581 gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg aatacgaaaa
     2641 tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat tcctgctaat
     2701 ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag aggagttgtt
     2761 cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg tgtgggtacg
     2821 gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa agtaatggct
     2881 gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa aacgaatagt
     2941 agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc cgagtttgcc
     3001 tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg taatgcgaac
     3061 ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa agtagacgga
     3121 attataggta ttacctattc agacattgat aaatatgtat catcaaattt accatttgtc
     3181 agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa ttatttaggt
     3241 gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg ctatgttggg
     3301 ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta caatatttgt
     3361 gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag caatttagag
     3421 gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt taccatcaat
     3481 gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga agcacctaag
     3541 gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga ctatttacta
     3601 tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac actcatggcg
     3661 atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt tcacgctgga
     3721 gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag gttgacagaa
     3781 agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt aattgaaaaa
     3841 taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa tgttagcaat
     3901 tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta aggagaaaag
     3961 ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg atgaaacgcc
     4021 tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg agggtgaata
     4081 taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg aggataaagt
     4141 aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg gaccgaatac
     4201 tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga aagatgcgga
     4261 catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca aaatgtatgc
     4321 gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt tcaaagatgc
     4381 tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga cggaatttca
     4441 agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata tgcaaatggc
     4501 gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga atggcggcga
     4561 tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa aatccgttga
     4621 agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca gtcctattga
     4681 aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga caatggcaga
     4741 tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg tttcagatgc
     4801 aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg agaaaacaga
     4861 gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat ctagtcgtat
     4921 tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt taattaaaga
     4981 tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg gacatgcccg
     5041 cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag ttcaagatat
     5101 tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg ctgctgaaat
     5161 gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca atcaagtgtt
     5221 aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc cagctttgct
     5281 gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg cctttactga
     5341 ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt ttaagcgact
     5401 gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg ttgtttggat
     5461 tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca aaaatcgtaa
     5521 aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct tagtcgttat
     5581 ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata cgctatttgc
     5641 aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa tgtatacgct
     5701 tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt tagggggaat
     5761 gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt ctaagtttca
     5821 gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta ttttacttac
     5881 cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc aaggtggacc
     5941 aatgaattct actatgacaa tggtttatta catttaccaa actggattta cagaccgtat
     6001 ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt taattacaat
     6061 tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt tggaccgcta
     6121 atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt cccgatgctg
     6181 tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt agcttccttc
     6241 aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca agaggttatt
     6301 tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt aacctttgcc
     6361 tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt taatttttca
     6421 ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat ggagacggtt
     6481 ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg cttggcagtg
     6541 attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt ctttatcgcg
     6601 attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt taaaattttc
     6661 tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt cttatccttt
     6721 atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag cagcaaattt
     6781 tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat caatcaagtg
     6841 atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt tgcacaaaaa
     6901 tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg aatagtgaat
     6961 gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa cagtcaatca
     7021 agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg atccaaatgg
     7081 ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt acgacagcat
     7141 ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact gggagcattt
     7201 gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt ccggcagtgc
     7261 catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg tggaagggca
     7321 agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg aaaaatatgc
     7381 cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg aagatttccg
     7441 cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta ctaaaactgc
     7501 agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt gggagtttta
     7561 ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc cagacttgtt
     7621 tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc cacaaggcaa
     7681 tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt ggcaaacagg
     7741 caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt atgcacccca
     7801 gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa tgtggcagcg
     7861 taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct tgcctagaga
     7921 gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt atcaagtaat
     7981 tgatccgcag gaaaggattg aatgggcgaa gactgaagag aatcgcttct tactgaagga
     8041 cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg caacgaaact
     8101 agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg atgttgaacg
     8161 ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag ctgaaaagga
     8221 agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa ccatcgaaat
     8281 tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta tgagtacaac
     8341 tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg aagtaacctt
     8401 ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg atttatggtt
     8461 caatcgaagc aggcggcaca aaatttgtgt gtgccattag cgatgaagaa atgacgatca
     8521 aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta gtcattgact
     8581 tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt gggcctatcg
     8641 atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa gtggcatggc
     8701 aaaattttga tttcatcggt accatgaaaa aagaatttcc gatcccgatt tcttggacaa
     8761 cagatgtcaa tgcggctgcc tatggtgagt atgtcttcgg cagtggcaaa ggattgagca
     8821 gtgtcgttta ctatacgatc ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct
     8881 ttatcgaagg gtttagtcat ccagaaatgg ggcatatgct cgtcgtccct catccaaaag
     8941 atgactttgc tggcagctgt ccatttcatg gcaactgttt agaagggatg gccgctggac
     9001 ccgccatcga aaaacgctta gggaaaaaag gacaagaggt tgcagaagat gatccttatt
     9061 gggaaatcga agcctcctat attgctcaat gtgcctacaa tacaaccttg atgttttctc
     9121 cagatgtgat catttttggc ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc
     9181 acgacgcatt tgcaaaattg gtcaatgggt atgtcgaaac acctgagtta agcaaatata
     9241 tcgtgacccc agcattagaa gacaacgctg gaacgctcgg ttgtttggca ttggctcgag
     9301 aagcggtctt acattcataa acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa
     9361 gatacaactg aaaatttagt cataaccagt taggcagagt gcttaactgg tttttgtttt
     9421 acttggaaga tcaagccttg aatgagataa gttcttgctc gaaagttatg gaaatttcga
     9481 tagccgtagg ctacttgtat tgtgcaatag ttttttacaa aaaaaagggg gtgcggatat
     9541 tttttttgga cctttttaac aaatatatct ttgcttcttc cgatattgaa gagggcttag
     9601 ccttcctaaa gataacttaa ttcgttgatt attataccaa tgaatataat catccaattt
     9661 gtttataaac tgattgattg ttgtattctt ccagtcacgt tgataaaaca tctcgttttt
     9721 taatcgtcca aagaaacctt cgcacgctga attatctggt gaataactct ttttagacat
     9781 agagtgaagc aagttggctt tgttccaata gtccaactaa caatagcacc atcgtaacaa
     9841 tcgacaagtg gtgataagta gattttccct gcgagaattc taaattcagt aatatctgtt
     9901 aaccattttt catttggctt ctttgcttta aaattccgat ttaataaatt ggggtgtgca
     9961 ggagaaattt ccccagcata cgaagaatac ttttttcgtc tagaaaattt aacaaataga
    10021 ttttcctcag tcatcaatct acgtacaact ttttcagaaa gctttttacc catatttttt
    10081 aaagcttggt gaattctacg gtaaccatac gcacagaagt tatgattgaa aatcgtgata
    10141 attaatttga cgttctttgt aatacttatc tggtttctca agtgcttgtt tttgataaaa
    10201 atagctgctt ttaggaaact ggagctgttg aagaagctct ttcaaggtga actgatttcg
    10261 taaggcatca atcaatatcg ttttttcttg atttgataga caaagaagat tgacgccttt
    10321 ttcttttttt agcagttcag ccgctccttc gagtaatgct ttttcgagct gtaattggtg
    10381 tacttgtttt tgtaagtctt ctacttcctt taaaagtagc tctttttgtt tttcctgatt
    10441 ttttaaagtc atacacaatg gaaaatcctt tccaagaacc gtgtttttcc attgatatag
    10501 agtaactcta ctaattcctc cagattcggc taaatttttc ctattagttt gcggtttata
    10561 gaattttttc aacacagctt ctttttgatc ttgtgttaac tttaccgcac tttttcggag
    10621 ttttctagct cctggtgcaa gttcttcaca ccgctgccgc aaggactctc tattaggata
    10681 accaagcatg cgaatggttc gtgcataaca ttgaccatat tcaaaataat gattcaccgc
    10741 tattcgtttt tgttcttcag aatactttga tttttttcgg tgctcctgat ggagctctcc
    10801 ggattccaaa tactctttat accatttccg tagtgcacca atcgacggat accctaattc
    10861 tcgaatggtt gcagcataag agcaatcgta ttttaaaaat aactgaattg cccttacacg
    10921 ctctttgtgg gaaaacacac ggacattcct cctaagttta ttttttcgtc caggattatg
    10981 tccgcacccc cttgctttct gatgacttat tctggttctt tgagtgtcaa tgcatagatg
    11041 tgatgtagct agttagaaaa aaattttgaa tactcttttt ttctttcaga ctccttttta
    11101 agtaagattt tgaagtgtga gttacggggt cagaaactta aaagtttttg atcctttatt
    11161 ttcaagtctg atttggcagc ttttcggcag ctcatttatt tatggtcaga aatagatgct
    11221 gaaattttaa tctattagca aatagtccag aacgtatgaa gaggagtcgg atatcatttg
    11281 tcattaacac cataaatatt tataaaattc gtgttcacct acaattaaat ttaatatata
    11341 atattcgttg taagggctta taattttttg ttaatgaaca agataggaag aaacatttaa
    11401 tggacagttt tcagagattg tcactagtaa atcaatttga gttattggct aaatttgagg
    11461 accaaaacag atcttattat gaaagaaaaa tcgaaatatt gagagaaggt tacgagtatc
    11521 attatgatga tgaaatttgg agtgatcttt ctgagccatt tccgaaagaa gattctcgat
    11581 ttgtattaga tgtattgaac atgtatcgag atatcaattt tagtaaatca aagctaaact
    11641 tagatgaaaa ccaaattact aattcatact atactcattt cggttctgtt gcaaagtttt
    11701 ccaaaaaatc tattttagtg taaaattgag aaaaaagaca gagaggacag agtaatgaat
    11761 cattttaaag gcaaacaatt caaaaaagac gtcattattg tcgctgttgg ttactacctg
    11821 cgttacaatc taagctatcg tgaagttcag g
//
LOCUS       DAFGFZ010000091        11836 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000091 DAFGFZ010000000
VERSION     DAFGFZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.093"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(81..821)
                     /gene="truA"
                     /locus_tag="LR726_002159"
     CDS             complement(81..821)
                     /gene="truA"
                     /locus_tag="LR726_002159"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL6217209.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1012..1647)
                     /locus_tag="LR726_002160"
     CDS             complement(1012..1647)
                     /locus_tag="LR726_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL6217210.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1794..3035)
                     /gene="sstT"
                     /locus_tag="LR726_002161"
     CDS             complement(1794..3035)
                     /gene="sstT"
                     /locus_tag="LR726_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL6217211.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3233..4231
                     /locus_tag="LR726_002162"
     CDS             3233..4231
                     /locus_tag="LR726_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217212.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4244..6262
                     /locus_tag="LR726_002163"
     CDS             4244..6262
                     /locus_tag="LR726_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBL6217213.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6322..7038)
                     /locus_tag="LR726_002164"
     CDS             complement(6322..7038)
                     /locus_tag="LR726_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL6217214.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7265..7807)
                     /locus_tag="LR726_002165"
     CDS             complement(7265..7807)
                     /locus_tag="LR726_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL6217215.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7812..8039)
                     /locus_tag="LR726_002166"
     CDS             complement(7812..8039)
                     /locus_tag="LR726_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL6217216.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8118..9998)
                     /locus_tag="LR726_002167"
     CDS             complement(8118..9998)
                     /locus_tag="LR726_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL6217217.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10187..10801)
                     /locus_tag="LR726_002168"
     CDS             complement(10187..10801)
                     /locus_tag="LR726_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217218.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10943..11503
                     /locus_tag="LR726_002169"
     CDS             10943..11503
                     /locus_tag="LR726_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6217219.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3696 a         2362 c         1902 g         3876 t
ORIGIN      
        1 ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa acatgcgttt
       61 catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag accttgtgca
      121 gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc gattgtttct
      181 atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg caccatttta
      241 tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat
      301 aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga aaaaccaatg
      361 aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat atccaactct
      421 tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt gttccaaaca
      481 taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc aggcatttct
      541 tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc gatcatttct
      601 gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca
      661 gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg atgcaatact
      721 ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca tcctgaatat
      781 ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg gctcctctgt
      841 cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata gctccttgct
      901 tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga aaaacaaaaa
      961 acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt tttactgttt
     1021 gagatgcaag atactcaacc aggcaatggg cacataatta aagaaaaaac tgattctttg
     1081 gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc ttgccagtcc
     1141 ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt ggcttacgtc
     1201 catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata aaaaaccact
     1261 gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg aagattcatg
     1321 ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga acgcgatggc
     1381 tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat acaatgcagg
     1441 caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg cttttcttac
     1501 aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta attcaggata
     1561 atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata ataaaaagaa
     1621 aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac ctatgctttt
     1681 aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta cacgaacatc
     1741 tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa cgttcaacgt
     1801 tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct tttacgcgcg
     1861 cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc agtttccatt
     1921 gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc gacatcatta
     1981 gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc cgcaacacct
     2041 gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa aacaaaaggt
     2101 gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt aatcgcggca
     2161 ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc ttttgtcaat
     2221 ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact tcgggtaaag
     2281 aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg attttcttta
     2341 ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat cgtaccgatc
     2401 aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt agcaattgat
     2461 tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg tacgacttgt
     2521 gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg tgctcgaaaa
     2581 gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc aataagagat
     2641 tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc caaactttgc
     2701 ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca
     2761 ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa aataggcttc
     2821 acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc
     2881 agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat tgaaataaat
     2941 gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac aacaatacgt
     3001 tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca caattttaac
     3061 aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa attctaacaa
     3121 tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg ttttttgaga
     3181 tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc ttatgttttt
     3241 gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga gttatggctt
     3301 cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga tttcagcagt
     3361 cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat ttttacaaac
     3421 ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat atcaaaaaat
     3481 cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc taatagccac
     3541 actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc gtgattattg
     3601 gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt gtactttgct
     3661 tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa caacaattaa
     3721 atatctacta ttatttttaa caattggatt agttggctgc tctactctat ttaataaaga
     3781 cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat ttttaatggg
     3841 ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc ctcaagtatt
     3901 tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt cactcatcat
     3961 cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct tcgcaatgta
     4021 ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag gtataaaagg
     4081 aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta atttcccttt
     4141 agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag ggaaggcatg
     4201 gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc tcctagttat
     4261 ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat acttcgcttt
     4321 tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc tttttgttca
     4381 taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc agattttcgt
     4441 tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa tatttactca
     4501 gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct tcttactatt
     4561 gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta acttactgct
     4621 cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc cttcagattt
     4681 aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct tattaatcgt
     4741 gggcggattg atccttctcc tattattgat tactagtatt attgtacaac gtagactaca
     4801 aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg ctgtattatt
     4861 aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt
     4921 taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa aagataacgg
     4981 accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac ctgctggcta
     5041 ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag atgaaatcaa
     5101 ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt
     5161 cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc cttacatcaa
     5221 aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg gtggcggaac
     5281 agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt ctgcttcgtt
     5341 agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata ttaccgatct
     5401 attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca gaaaacaagt
     5461 gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata aattaactta
     5521 tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca aagaaacctt
     5581 gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca ctatgcaaaa
     5641 ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag gaactgctgt
     5701 cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact ataccgatac
     5761 agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta cctttgtttt
     5821 ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat atggattaga
     5881 acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac aagcagctac
     5941 gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg agcatgcgaa
     6001 tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc tacctgcatt
     6061 aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg tctttgttac
     6121 agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag aaattctaga
     6181 agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg caacatgggc
     6241 gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata aaagacatgt
     6301 cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat taaagccatt
     6361 tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat tcccctgatt
     6421 ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat agacgtgctt
     6481 tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat agtctgcttg
     6541 acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag ccatcttcca
     6601 ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac tcatttgatt
     6661 cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa atgcctctaa
     6721 ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc tttctgaaga
     6781 ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt cagtcgtttt
     6841 ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag ttgacttgct
     6901 ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt ttgttgattc
     6961 ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac caattgttac
     7021 taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct ttattctatc
     7081 aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt caaatcttct
     7141 aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt
     7201 ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga agaaacatag
     7261 acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt ccttggagtc
     7321 tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc aattgttgca
     7381 agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag acattttgga
     7441 aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat ttgttttgac
     7501 catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga acgatttcat
     7561 tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga tcagcatatt
     7621 ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat tgtgcttgga
     7681 tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg gcgccagcag
     7741 ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt tcttccacta
     7801 tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc taatttttcc
     7861 acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc tttgattttg
     7921 ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac cgcatgttcg
     7981 attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat ttttttcatc
     8041 gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc cataaaaaaa
     8101 ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa atacaataaa
     8161 cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat cccactagcc
     8221 atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc aatggcaatc
     8281 actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc atgaaccaac
     8341 gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac atccgttcct
     8401 gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt gattccatct
     8461 ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg aaccttttcg
     8521 tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac ataatcagca
     8581 gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg tttcaactta
     8641 gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat ttgagtgatt
     8701 tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg atgtaaatct
     8761 tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt gttgcccgca
     8821 caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt ctgattatct
     8881 ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat cacggcgcgc
     8941 cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct gtccccttca
     9001 tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa tgtaccagtt
     9061 ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc gcctttgact
     9121 aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg cgcaccaatg
     9181 actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa atcacgacta
     9241 atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg agaaaatcga
     9301 tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa ttcgataatt
     9361 ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta gccatctgct
     9421 aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg tgattcacca
     9481 gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc cacaggaata
     9541 gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat ttccacttct
     9601 tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc ggtcaaagcc
     9661 ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac
     9721 aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc aagcacagca
     9781 atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata cgcatggatc
     9841 ataataggta tgaccgcaat aatagaagct atggctagag caatgttata gaacaagaca
     9901 aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa ggcggtgata
     9961 tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct tttcctttca
    10021 ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac tatcaagttt
    10081 agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta gcaagaagac
    10141 tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat tgaatgattt
    10201 tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc ttgctaattt
    10261 tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag ccactatctg
    10321 taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc aacttggtac
    10381 cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct ccatctggcg
    10441 aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa gctcctttac
    10501 tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa
    10561 aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct gccaactcgt
    10621 cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac aaaaaatatc
    10681 ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc attccttcaa
    10741 cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa gttagtttca
    10801 tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata gacacagtgt
    10861 ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt tgctttgata
    10921 tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa
    10981 agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag caatcactat
    11041 acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac attataagga
    11101 tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa atgaaattct
    11161 ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg actatgttta
    11221 cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt cgtttcagaa
    11281 gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg aagacaagaa
    11341 aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag gttgtctatc
    11401 cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat tattagaact
    11461 cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa catactaagc
    11521 ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta gccaaattgt
    11581 ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa gtagtttgta
    11641 gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt cacctcatca
    11701 ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg taaccaattt
    11761 atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta cctcaattca
    11821 ttttcaaggg gaatca
//
LOCUS       DAFGFZ010000092        11481 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000092 DAFGFZ010000000
VERSION     DAFGFZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.094"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            151..1410
                     /gene="lysA"
                     /locus_tag="LR726_002170"
     CDS             151..1410
                     /gene="lysA"
                     /locus_tag="LR726_002170"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL6217220.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1397..2197
                     /gene="dapF"
                     /locus_tag="LR726_002171"
     CDS             1397..2197
                     /gene="dapF"
                     /locus_tag="LR726_002171"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL6217221.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILGE"
     gene            2200..3081
                     /locus_tag="LR726_002172"
     CDS             2200..3081
                     /locus_tag="LR726_002172"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL6217222.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3078..3794
                     /locus_tag="LR726_002173"
     CDS             3078..3794
                     /locus_tag="LR726_002173"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL6217223.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3800..4495
                     /gene="dapD"
                     /locus_tag="LR726_002174"
     CDS             3800..4495
                     /gene="dapD"
                     /locus_tag="LR726_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL6217224.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4511..5512
                     /locus_tag="LR726_002175"
     CDS             4511..5512
                     /locus_tag="LR726_002175"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL6217225.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5509..6870
                     /locus_tag="LR726_002176"
     CDS             5509..6870
                     /locus_tag="LR726_002176"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL6217226.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6830..7981
                     /locus_tag="LR726_002177"
     CDS             6830..7981
                     /locus_tag="LR726_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL6217227.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7956..9125
                     /locus_tag="LR726_002178"
     CDS             7956..9125
                     /locus_tag="LR726_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL6217228.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9300..10679
                     /locus_tag="LR726_002179"
     CDS             9300..10679
                     /locus_tag="LR726_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL6217229.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10960..11481
                     /locus_tag="LR726_002180"
                     /pseudo
     CDS             10960..11481
                     /locus_tag="LR726_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3819 a         2012 c         2558 g         3092 t
ORIGIN      
        1 aaccattttt attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa
       61 ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa
      121 cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca
      181 gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg
      241 agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg
      301 ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga
      361 ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct
      421 agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt
      481 gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa
      541 ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa
      601 gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat
      661 gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta
      721 gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa
      781 atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc
      841 ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta
      901 aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa
      961 ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt
     1021 ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat
     1081 aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg
     1141 gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta
     1201 attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg
     1261 ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt
     1321 tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta
     1381 aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt
     1441 attagattac gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga
     1501 aaaatatgat atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta
     1561 caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata
     1621 cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac
     1681 aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc
     1741 agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat
     1801 agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt
     1861 tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca
     1921 tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga
     1981 attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg
     2041 ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt
     2101 tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc
     2161 agcagttcat gaatggacaa gaatattggg ggaatgaaga tgataaaagg ttcgatcgtg
     2221 gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg
     2281 atccaattcc atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa
     2341 tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg
     2401 gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa
     2461 aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac
     2521 aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg
     2581 attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta
     2641 ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg
     2701 gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat
     2761 gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg
     2821 aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag
     2881 caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc
     2941 aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc
     3001 ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa
     3061 gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag
     3121 tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag
     3181 aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag
     3241 ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca
     3301 cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg
     3361 tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag
     3421 cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa
     3481 aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc
     3541 tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca
     3601 gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa
     3661 tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag
     3721 cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg
     3781 acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc
     3841 taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag
     3901 taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc
     3961 ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata
     4021 cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat
     4081 tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa
     4141 tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg
     4201 agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga
     4261 accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt
     4321 gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt
     4381 agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag
     4441 tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag
     4501 atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca
     4561 aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc
     4621 ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga
     4681 attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt
     4741 atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc
     4801 agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta
     4861 tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct
     4921 taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt
     4981 ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc
     5041 agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga
     5101 aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca
     5161 gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt
     5221 ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag
     5281 ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat
     5341 tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga
     5401 gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc
     5461 cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg
     5521 ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc
     5581 cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa
     5641 cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag
     5701 catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga
     5761 gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg
     5821 gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct gactgctcaa
     5881 ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac
     5941 tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga
     6001 tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta
     6061 ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa
     6121 agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat
     6181 catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg
     6241 gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa
     6301 ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa
     6361 aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc
     6421 aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa
     6481 catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca
     6541 gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc
     6601 gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat
     6661 aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc
     6721 acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat
     6781 caatatgaaa cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga
     6841 atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa
     6901 atatttatat gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg
     6961 actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga
     7021 tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat
     7081 gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac
     7141 taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat
     7201 ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc
     7261 aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg
     7321 acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat
     7381 tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt
     7441 ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat
     7501 ccaaggtgga acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg
     7561 ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca
     7621 gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa
     7681 ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc
     7741 agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt
     7801 tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt
     7861 tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa
     7921 aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta
     7981 gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt
     8041 cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg
     8101 tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag
     8161 gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt
     8221 atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt
     8281 tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat
     8341 atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag
     8401 ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca
     8461 aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat
     8521 tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat
     8581 atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa
     8641 caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta
     8701 tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg
     8761 ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata
     8821 gcgagaatat gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc
     8881 ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag
     8941 ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg
     9001 cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg
     9061 ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac
     9121 agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga
     9181 gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt
     9241 aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa
     9301 tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg
     9361 aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata
     9421 tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg
     9481 atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc
     9541 acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata
     9601 acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca
     9661 atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg
     9721 caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg
     9781 cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat
     9841 tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt
     9901 tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt
     9961 ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc
    10021 tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact
    10081 tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt
    10141 atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg
    10201 ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag
    10261 taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc
    10321 attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag
    10381 tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa
    10441 aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat
    10501 tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac
    10561 tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat
    10621 ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag
    10681 aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca
    10741 gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    10801 gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc
    10861 caaaagttag accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac
    10921 ttttgaactt aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt
    10981 aaaaatccag atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat
    11041 tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag
    11101 aaagttcagc gcttgatcaa gaagctagga ataaaagtaa cggcatacac gcgtaaatca
    11161 cgtcgctaca attcttatcg aggaaaagtt ggagcagtag ccaaaaatcg gatccatcgt
    11221 cgtttctaca ccagtatctg ccatcagaaa atcacgacgg atacaactga atttaaatac
    11281 catgaagtgg atgccaaagg cattatccgt caaaagaaat tgtatctaga tccttttatg
    11341 gatatgttca atagtgagat tttatcctat cgtatctctg aaaagcccaa tgcattagcg
    11401 gtaatggaag gtttggagga agctatccag accactaacg attgtcccta tcgtcgtacg
    11461 tttcactcag atcaaggttg g
//
LOCUS       DAFGFZ010000093        11382 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000093 DAFGFZ010000000
VERSION     DAFGFZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.095"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            40..1680
                     /locus_tag="LR726_002181"
     CDS             40..1680
                     /locus_tag="LR726_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL6217230.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1834..3156
                     /locus_tag="LR726_002182"
     CDS             1834..3156
                     /locus_tag="LR726_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL6217231.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3218..3649)
                     /locus_tag="LR726_002183"
     CDS             complement(3218..3649)
                     /locus_tag="LR726_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217232.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3713..4549)
                     /locus_tag="LR726_002184"
     CDS             complement(3713..4549)
                     /locus_tag="LR726_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL6217233.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4739..5041)
                     /locus_tag="LR726_002185"
     CDS             complement(4739..5041)
                     /locus_tag="LR726_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL6217234.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5067..5498)
                     /gene="ruvX"
                     /locus_tag="LR726_002186"
     CDS             complement(5067..5498)
                     /gene="ruvX"
                     /locus_tag="LR726_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL6217235.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5495..5761)
                     /locus_tag="LR726_002187"
     CDS             complement(5495..5761)
                     /locus_tag="LR726_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL6217236.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6015..6740)
                     /locus_tag="LR726_002188"
     CDS             complement(6015..6740)
                     /locus_tag="LR726_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL6217237.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7106..7176)
                     /locus_tag="LR726_002189"
     tRNA            complement(7106..7176)
                     /locus_tag="LR726_002189"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7142..7144),aa:Gly,seq:ccc)
     gene            7293..8045
                     /locus_tag="LR726_002190"
     CDS             7293..8045
                     /locus_tag="LR726_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL6217238.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8061..8540
                     /locus_tag="LR726_002191"
     CDS             8061..8540
                     /locus_tag="LR726_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL6217239.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8791..10371)
                     /locus_tag="LR726_002192"
     CDS             complement(8791..10371)
                     /locus_tag="LR726_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL6217240.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10364..11107)
                     /locus_tag="LR726_002193"
     CDS             complement(10364..11107)
                     /locus_tag="LR726_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217241.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11283..>11382)
                     /locus_tag="LR726_002194"
     CDS             complement(11283..>11382)
                     /locus_tag="LR726_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBL6217242.1"
                     /translation="VTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3458 a         2276 c         2039 g         3609 t
ORIGIN      
        1 ctatacttcg tttgtattaa aacttaggag gaatcattca tgcttacagg gctaaactta
       61 ttaaataatc cttttctaaa taaaggaaca gcttttacaa aagaagaacg ggcaaaatac
      121 ggaatcactg gtatgcttcc aagtactgtc caaacattgg aacaacaatc tgtccaagct
      181 tacggtcaat acttaagcaa acagaccgat ttggaaaaac gtatcttctt aatgaatcta
      241 ttcaatacga accggacatt gttttataaa ttgatgggcg aacatttagt tgaattcatg
      301 cctgtcgttt atgatcctgt cgttgcggat tcaatcgaac aatacaacga aattttcttg
      361 aaaccacaag atgctgtatt tctttcaatc gacgaaccag aaaatatcaa agcaagtttg
      421 aaaaacgcag cagatggacg cgacattcgt ttgatcgttg tgacagatgc agaaggaatc
      481 ttaggtatgg gtgactgggg tgtgaacggt gttgacatcg ctatcggtaa attgatggtc
      541 tacactgccg cggctggtat caatcctgca caagtcttgc ctgtatcaat cgacgctggt
      601 actaataacg aagaattatt gaataatcca ttatacttag ggaatcgtca tgctcgtgta
      661 gaaggcgaaa cttactacga attcattgat caattcgttc aatcagctac tgaattattc
      721 ccagaattgt tattacattg ggaagatttt ggacgaggaa atgctgcaac gatcttagaa
      781 aaatacgaag ataaaatcac tactttcaat gatgatatcc aaggcacagg aatcgttgta
      841 ctagctggtg tattaggtgg attgaatata tcaggtgagc aattgaaaga tcaaacgatc
      901 ttgacatttg gtgctggtac tgctggtgta ggtatcgcaa acatcctttt agatgaaatg
      961 atccgtcaag gtgtacctga agaagaagca cgccaacatt tctatcaagt agataaacag
     1021 ggtttattgt ttgaagatac aaaaggattg actcctggtc aaatcccatt cgctcgtaaa
     1081 cgctcagaat tcactaatgc tgatgaattg acaaacttag aagcagttgt aaaagcaatc
     1141 catccaacga tcatgatcgg tacttctacc caacctggag cattcactga agaaatcgtg
     1201 aaagaaatgg cggctcatac cccacgtcca atcatcatgc ctttgtctaa tccaacaaaa
     1261 ttggcagaag caaaagcaaa agatttgatt gaatggacgg acggaaaagc tttagttgga
     1321 acaggtatcc ctgctgctga tgtggaatat aacggtgtaa cttaccaaat cggtcaagca
     1381 aataatgcgt tgatgtaccc aggtttaggg cttggtttga ttgcttcaac agctacacga
     1441 gtaaatgctg aaatcatttc tcaagctagt cgagcattag gcggaatcgt tgatgtaaca
     1501 aaaccaggag cagctatctt gcctccagta gcaaaaatca ctgaattctc tcaaacgatt
     1561 gctgaaactg tcgcaaaatc agttgttgca caaaaattga atcgtgaaga aatcacggat
     1621 atcaaagaag ctgttgaatc tgctaaatgg gttcctgaat ataaatcttt agaagattaa
     1681 gattgttata gtaaaagaac atacagaaac tttgtatgac gtctaaaaag aaaaaagcga
     1741 ctgcggcaaa acagcagcca gccaactact gctcttttgc cgcttctctt tggctttttt
     1801 catacgatac agaataagaa aggatctgta aaaatggcac aaaaaacaac aaccgcaact
     1861 gcagttgagg aaaagaaaaa agaagggttt tggtcaacaa aaattaccgg cgtaagtttg
     1921 cccctttatt tgatcatggt ccttgtcctg atcattgcaa tggctttagg aaaattaccg
     1981 acgaatatga ttggcccaat cgctgttttg gtcattttcg gtaatttatt tcattttatc
     2041 ggaacaaaaa tccctatagt caaaagttat ttaggtggtg gctccgtttt tgcgatcttt
     2101 gcctctgccg cgattgctac atttgggatc ttacctgagt atgtagtaaa atccacagaa
     2161 aactttgtca gcaatatggg ctttttagac ttttatattg ctgccttgat tactggtagc
     2221 atccttggta tgaaccgtaa tctattgatg aaagcttctg ttcgttttat tcccgttgca
     2281 cttatttcaa tggtcgtctc cttttttgcc gtaggactcg taggtatgct tatcggaaat
     2341 ggttttgcaa attccgtttt atatatctca ttgcccgcaa tggctggtgg tgttggagcc
     2401 ggagctgtac ctttatcaac gatttatgca aatgttttag gtacggacgc ttcttctatc
     2461 atttcccgcc tgattcctgc gacagcaatg acaaatgtcc tagctatcat tggtgcagcg
     2521 ttagttgctc gtgcgggaca atcaatgcca aaattcaacg gtaatggtaa attaatggaa
     2581 aaaggcgacc ttggcgatgg aaaacctaga aatgtaaaac cttccattcc acaattaggt
     2641 gtcggtttga tggtatcatt gactttcttc atcttaggac aaatctgtaa ctattttgtt
     2701 cctaaagtcc atgcttacgc attcatgatc attatcgttg ttatctgtaa aatcgcaaac
     2761 ctattaccag aatattacga agatgcagct atcatgttca acaacttgat tgtcaaaaac
     2821 ttgacagcag ctgttcttgc cggtatcggt attgcgttat tgaatctaaa tgtattggct
     2881 tctgctttga catggcaatt tgttgtctta tgtttgacaa gtgttatcgc gatttcagta
     2941 gtttcaggat ttgtcggtcg tttgtttggt ttatatccaa tcgaatcaac tattactgct
     3001 ggcttatgca acaactcgat gggtggtaca ggaaatgtag ctgtcttatc tgcagctaat
     3061 cggatggaac ttattgcttt tgctcaaatg ggcaatcggc ttggtggtgc catcgttttg
     3121 atcatctcag gtttcttgat gcaattactt tcttagtcaa aaaaaatacc tgatcatcaa
     3181 gtatcggctt gatgaccagg tattttttga taaaaattta caggattttc aaaaatcttt
     3241 tttgcatttt atcggaatga taaccgatgc gttcataaaa tcggtgtgct tctactctgt
     3301 attctgcaga attaagtctt attgctgata ttcctcgtct tttcgcttct tctacgccag
     3361 tcatcaaggc ctttccgatt cctctttttt cagcatgttt agacacagca agtgccataa
     3421 tattgaacat cggatcgctg aaaatcgatt cataggactc cgcttgtaca tagccagctg
     3481 cttttttaga agtaggatct tcatagacca agaaaaaatt tttttctggc tccttcaaca
     3541 gtttcttcat ttgtctcacc gtaacggtta atggtacatc atatcctaat tgttccgcat
     3601 tgatttctcg tagtgcttct gcatctccta aacgcatttc ccgcaacatc caaatcacca
     3661 gccaattttt atttttttaa atgaattttt ttatcacagt atactccttt tttcacttaa
     3721 aaaaagactt ttcagtgatt ttgacttgct tgaatccaga tccttctaat gtttgcaaga
     3781 tgacgaaagc attgtaaaga ttgatcaaaa gaaccacgat gacaatcgct tgaaaaataa
     3841 aggtcacttt ttttggagac aaaaccctgc taaatcttgc tccccacaaa cctccaataa
     3901 cagcagcggg gataacataa aacaacatag ataaatcaaa gcgcataaag ctacttgtta
     3961 aagcaatcgt taccaatttt gccagttgag aaaagaaaat cgtagataga gaatatagtg
     4021 ttgcttcttt tattggcaat gcaaacatca gcatcaacaa agaaacattg actggtccac
     4081 caccaatacc taaaaaacta gctaaaaagc ccagaacccc cccacatata aggtaccaag
     4141 tccatgacgt caaatgaaat tttggctgat gatgcttggt gtagaagaac gcaaaaacca
     4201 aggtcaatac cgtcaaaaaa atttggatca gctgcacatg cttctcgttt tcaaataact
     4261 gtaaaaaaac ttcgaataca atgttgccga ctactcctcc aacaacagct ccactagata
     4321 cccaaccgac aatctgccaa ttcagtgatt tcccgcttga tacttgtcgc acagtcgaaa
     4381 caatggacat cgtgaatacc gcaactgttg agtaaaaaga aatccctgca acagaatggg
     4441 cgccgataag atcaagaatc ggtttgatca acacgcctcc acccatcccg gaaatagcgc
     4501 caactgtgtt cgccagaacg atcaccacaa aataaatcag accaatcatc ttgtatttcc
     4561 tttctcttgt ttatatcttt tagcgattgt tactcttttc ttttattata cagtaaaaga
     4621 aacaaacgag gcttcgaaag cttttctact ttttaaggaa atttagtgct aaacgaaaaa
     4681 accgggctct acttttcaga gagtccagct tttcttacac cttatcgatt cttttgttct
     4741 attcttcttc cgccatgaat gtattgaata cttcttcgat catatcccat tctgcgtctg
     4801 tttcgatttg ttctaactcg ccttctgttc catcttcgtt ttcgatgtat gcataagctt
     4861 gtaattctac atcttcatct tctgaaacac cagctggata taaaagaaca tagttacgtc
     4921 caaattcttc ttgtccgtca actgttagca agatttcgta taatgtttca tttccttggt
     4981 catccaccaa tgtgatgtgt tcgtgtcctt cgtgttcgtg ggaatggtta tgttctgtca
     5041 taagattacc tcttttcttt ttattattaa aataattgct tacctgagcc gtctaaataa
     5101 ttttgtaaaa tcatcacagc tgctaattta tcgatgactt tttttcgttt cgctcttgaa
     5161 gtatttgcct gctctactag catacgttct gcctgaacag tggttagtcg ttcatcttga
     5221 taatcaaccg gcaaggagaa tagttctttg atcttctctc catatgacat ggaagcttct
     5281 gcccttggtc cgatggaatt gttcatattc ttaggtaatc caactacaaa acggtctact
     5341 tcgtactgcg ccactaattc cttcaaccga tcaaaaccaa actcttcttc tgcctcattg
     5401 attcgaatga tttctactcc ttgagctgtc cagccaaagg gatcgctcac agcaacgcct
     5461 actgtcttag aaccgacatc caagcccatt actctcataa attcactcca tgatttgaga
     5521 ggtagtcttt gacaattact tccatgattt catcgcgttc gtgacggcga atcaaatttc
     5581 gtgcatcacg ataacgagga atgtaggccg ggtctccaga aagcaagtag ccgacaatct
     5641 gattgattgg attgtagcct ttttcttcca aagcccgata aacaatcgct aatgtttcgc
     5701 tcacttcttt tttgcgacta tcgtcaaaac caaaacgtac ggtttcatct gtaaaaccca
     5761 tgattctaca cctctttccc gcaaggtaat tccagcttac cttctacacc cttattttac
     5821 tcgaagaaag ggaaaactac aaacaaatat tccaaacttc tgaataaatt aagaaacacg
     5881 atttgtctga tttttccttt tttctgtggc tttttcaaaa gccctctttc cataaagtat
     5941 acgctatatt tctttgaaag tatgttttta cacaattttt tcataaagtt cataaaaaat
     6001 tcgaattgct gctactaatt tttcgttttc gttgcttgcc ctgtctcata attgatggtg
     6061 tagccatctt gaacattgta gataaagaca ttgaattcaa tttttttgtc ttcaatgctt
     6121 tctgcttcca actgcacacc tcttgctacc agttcatttc cctcgaaatg aggagtgaca
     6181 cgatagcgta catgatgatt tgtttccttg atatatgccg caatatcatt ttcgtgggca
     6241 agcatgtacg gagcattcaa cgaacgtgtc cctgtcatta agtttttaat gttattattt
     6301 tgaccagtca actggaaacc aatcaaatgg ctgcggttgt ataaccattg tccgtcactt
     6361 aatttctttt gtttccaacc tgtcggatca atatacaaag gttctctctt ttcagtagga
     6421 aacaagtctt ttcccaacat cgcattggct tgtcctactc tgttcaattt gtctatattt
     6481 gaatagcttt cccatgtccc tttggaaaga ctaagatcct cttttgtaaa ctccggctga
     6541 ttcccattga tttctatttc ttgatgtttt ccatcatatt ctggcacatc cgctacttga
     6601 gaagcttgcg gtgtggaatc aaaaatggtt gtaatttgtt ggaagatatc tgaatctttt
     6661 tgtgtggtgg aaataccgag tgcccctgca atcacagcag cgacgatccc cacgatcgcc
     6721 ttggttgttt ttttgctcat ttctttactc ctttactaac agttccctaa atcttaacat
     6781 atgttcccct ataaataaat actggaaaga aatagaaaca gtaaaaaaat aaaaccagtt
     6841 tttagacggc ttaagaatac aaaaaaagag acggattcat cgtctctact caatcacaaa
     6901 acactattta caaggtattt attatcattc aaaaaatttt tcttaatact caaaactaaa
     6961 cggcttctgt acgacctctt ggtaaacagt gttcaaaagc ttgcatctgc ggtaataagt
     7021 ccaaccaagt caaaaacata agaagctttt ttgcttgact attcttatta cttgatgcag
     7081 ggcagctttt tcacagcctc ttttaagcgg gtgacgggaa tcgaacccgc gacatcagct
     7141 tgggaagctg ggattttacc actaaactac acccgcgata caaaaacagt atacgcctct
     7201 ttcactcctt cctcaagttt ttttattctt tacgcttccg atatgttttg tagtaaaata
     7261 aaactaatca atcaattatt ggagaagatg ctatgacggg aaaagaatta gatctgttgc
     7321 tggacacttg tttactcgct gggaaaatca tgatggaaag caatgcggag atgtatcggg
     7381 tagaagatac gatgagtcga attgctttag cttctggaaa tttccgtttg gtcagctatg
     7441 tcactcaaac cggtctcttt attggtcttg atcgtacctc gaccatccga atggaacaaa
     7501 ttaccaaccg ttcgattaat ttagaaaaag tagtaaaagt aaatgatctt tcacgtaaat
     7561 atgttgcttc agagctaaca ttagaagaac tttactgcga actgcaagca atcgaccgag
     7621 accgttcttt ctttcctgtc tggttgcaaa tcgttagcgc ggcagtgatc agtggaacaa
     7681 ttatggtgtt atttggcgga aagatgaccg atttacctgt gactttagtc attggaggat
     7741 tgggatacgc agcttatcta tatagtttga aattttttag aatcaaattt ttatcggaat
     7801 ttttatcttc tttattgatt ggcatagccg ctatctacag cgtacgtttt ggcttaggaa
     7861 gcaatcagga tctaatcatt atcggatgtg tgatgccttt agttcctgga gttcaaatta
     7921 cgaacgctat ccgtgatttg ctagctggac attatgtctc aggagtttct cgcggaacag
     7981 aagcgatgat gactgctacg atgatcggtt ttgcgattgc ttttattttc cagctttttt
     8041 attaggaaag gaacacaagt atggggacga tatttattca gttttctttt agttttttag
     8101 cgactgcggc atttgccgtt attacaaatg tacctaggcg ttcattgatt tgttgtggtc
     8161 tttctggtgc ttttggctgg atgatctact ggatctgtgt tcaaataggc ggatctgctg
     8221 cttttggttc attgcttggt tctttaggag tagcattcat cagcgatctt ttctctaaac
     8281 gtttgaaaat gcccgtaacg attttcaata ttcccgggat ggttcctcta gtccctggtg
     8341 gtttagctta tcaagcggtt cgaaatctcg ttactgaatc ctatcgagaa gcggctcatt
     8401 atgcggtcca agcaattatg attgctggag caatcgcttt aggtcttgta ttatcagaag
     8461 tctttaacca taacatccgt aatttcaaag taaagcgtga cgcaatcatg atgaaacgaa
     8521 aaaaacggct ttttaaataa acgaagtgta acaaagaaaa ggttgtgaaa gagacgttca
     8581 gccttgagcg atgacaaaat atctcacaaa cattgcgaca agtaaccgca ggagcaataa
     8641 gaagtaatta tgagtattaa aaaaagtttt gtaaaaatag cttctcacat ttttacataa
     8701 aattgactta atactgagaa actgactttt gaacaccctc tatctgtaaa aaaggggctg
     8761 cgacatgatt tttgtcacgg cccctcttct ttattcaaat tttttccaaa acgttcgttg
     8821 ataataaacg atccatagag cggaaagtat tagaactaaa ccgatcacag gaatattcac
     8881 taaccaaaat ggataaaaca aagccaacat tccgtataag acgataagaa taatactgat
     8941 gatcaatgaa cccatcatag ttgcgactaa acccaaactt cctgaacctc tgttaaataa
     9001 ttggttaata ttggtccagt tcagcagcag caaccgataa tctctagcga aataacgtag
     9061 tgacaataag tagcagccga ataaagaacc aaatagcaag ctagggataa aaaacaaagg
     9121 cagctgaaaa gacagtccgc ttaataatgc gatcccacca gttaaaaaac tctgcaatat
     9181 ccatccgaat ctgaactttt cttttaaata ttggttcata gagattggca aagaacggat
     9241 aaacagaaaa ttttcttgat ccaaagaaat aagattgctg ataaaagaag tctgattaac
     9301 tgttaagaaa gccaatgcga tccctaccag gaaaacaaca ccgattaact tattatctaa
     9361 atcagttaat tttatttcgc ctcctagggc aaaagcaaga ataaaaatga ttggagttaa
     9421 caaggaagaa gaaagaactt gcataatcaa attaggttct tttactaatt gagcattgta
     9481 actaaataaa agctgatgca cattttggtt ctttttatgg gttcttttta ttttgaactc
     9541 actgtttgaa tcagtcagtt gctctgtaag ctttggtact atcagctgtt ttacagcaag
     9601 gaaaaatccc agagccgtga tagctagtat tccccaaccc aataaacctt gttgagaaag
     9661 tggctgactg gcagcatcat agaatggcaa taatatcgaa atcggatgcc gatctgttaa
     9721 ctcattttca atagtccccg tttgatgatt catccacatg attccagcaa atgcgatcag
     9781 catcgagctg ccaagcaaga tactagaaac tgtttttcta tgttttttaa acactgctgt
     9841 tcgagccaaa ccaaaaacaa tcaaactagc cagacaaaaa agaataatca aaaatagaga
     9901 aaacaaaagg attgagataa tgatagtaag caaaatccct agatcagctc gccatccggt
     9961 taaaataaac atgactagaa gcggaaaaac aaaaggaatg atggtcaaag caactaccaa
    10021 aatctttgct gaaaaaacct gttcttgtcc aaaaggcaga ggcaaaaata cttgcaggtc
    10081 atttccttca aaaaagatat tataaatgac tgatatactt tgagttaaac ctaacatacc
    10141 aaaaagtgcc acgtaataag taaagaaacc tggcaattta acaaagttca tagcaaacat
    10201 agcaaatccg taaatcagta aaaaaatcag ccctgaaaaa agattttgag acaaaagata
    10261 gatagttaat cgctttccct tttttccttt ctctcttgct ttattcgtga gctgtggatt
    10321 aacatataaa agattaactc gaattaattc taataattgt cgcttataca tacggtccat
    10381 ctcctgtcgc ttgccttcca gccattttca aataaatctt ttctagcgtt tcatcaggtg
    10441 agccattcaa taattcacta actgccccct gataaatcaa ttcacctttc ttcaaaatag
    10501 ccagtttatc gcataactgt tgtgcggtat ccaatacatg tgtagaaaaa atgactgttt
    10561 ttccttttgc ggcatgagcc ttcatcattt ctttcaaatc aaatgctgct tgaggatcaa
    10621 gtccttgcaa aggttcatcc aatacccaaa tatctggatc tggcagcaac gtgccgatca
    10681 aaatcgtttt ttgacgcatt ccatgtgaaa aacttgcgat tgtttcattt tgatgactat
    10741 acatatcaaa aagcgctgtc aattctgcta aacgcttttc ttttttttgt gtgtctaatt
    10801 cataggcagc tgaaatcaag tcccaatatt ctccagccgt caattgtaaa aaaatatctg
    10861 gggaatcagg cacataaccg attttttgtt tgatactcaa tcgattttcc gtcaatttca
    10921 ttccatctac taaaatcgtt cccgatgatg gttcaataat actaactaaa cttttaatcg
    10981 tagttgattt tccggcacca ttatgtccta aaaatccaaa aatttcccct tgtcttatcg
    11041 agagagacaa gtcttttaat gcttgttttt tcccgtaact tttacttaca ttgatgaatt
    11101 caatcatttc tgtactcctt ttgtttgtta ttacttttta ttgtaccctt cgttattggt
    11161 ggagtaaatg tcaaaaagag tgcataacaa aaaaggatag aaacgactag gctgctactt
    11221 gggttgttct cttctacagg aaaacactct tctaacaggt aagatatgag tctttgtttt
    11281 ctttaaaatg attcattttc tatcttttct tgcataaaat tctttagttt atcaatactt
    11341 ctctttccca tctctagttg ttctccattt gcaaatgtta ct
//
LOCUS       DAFGFZ010000094        11078 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000094 DAFGFZ010000000
VERSION     DAFGFZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.096"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1305
                     /locus_tag="LR726_002195"
     CDS             <1..1305
                     /locus_tag="LR726_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217243.1"
                     /translation="YTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKV
                     IALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTC
                     ALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVA
                     LIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIF
                     FSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEE
                     TLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASN
                     KKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMA
                     LWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1292..2926
                     /locus_tag="LR726_002196"
     CDS             1292..2926
                     /locus_tag="LR726_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217244.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3101..3934
                     /gene="msrA"
                     /locus_tag="LR726_002197"
     CDS             3101..3934
                     /gene="msrA"
                     /locus_tag="LR726_002197"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL6217245.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4047..5003
                     /locus_tag="LR726_002198"
     CDS             4047..5003
                     /locus_tag="LR726_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL6217246.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5292..5990
                     /locus_tag="LR726_002199"
     CDS             5292..5990
                     /locus_tag="LR726_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL6217247.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6140..7456
                     /locus_tag="LR726_002200"
     CDS             6140..7456
                     /locus_tag="LR726_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217248.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7473..8888
                     /locus_tag="LR726_002201"
     CDS             7473..8888
                     /locus_tag="LR726_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL6217249.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            9352..9672
                     /locus_tag="LR726_002202"
     CDS             9352..9672
                     /locus_tag="LR726_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217250.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            9698..10015
                     /locus_tag="LR726_002203"
     CDS             9698..10015
                     /locus_tag="LR726_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL6217251.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            10044..>11078
                     /locus_tag="LR726_002204"
     CDS             10044..>11078
                     /locus_tag="LR726_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217252.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         3687 a         1763 c         2222 g         3406 t
ORIGIN      
        1 tatacttttg caacttatcg aacagttgat atctattatc gatccgcaat tgcagaagca
       61 atcgctctta cgattatccc agctgtttta tattatgctt atggattgat attcaataga
      121 gaaaaaaaag tcatcgcttt atctgtcagt ataagtctac ttgtgtatac tcatgtctta
      181 actacactat tgtgtagtgc attgattgct tgtatgatta tagttcgttt gtttttaagc
      241 aagcaaataa aaaacgatat aaaatttgtg gtcgtgaatt tagtaaaggc tgtttttttg
      301 acttgtgcat taactagcta tttttggtat cctatgttgc agcagatgaa ttatcaagaa
      361 atacacagtc ccttcaaaac agatttgcag ctagaagcac tttctatttc agatagtatc
      421 attcaagcgt taaataacga tattacaaca tacactatgg ggttattagg attggttgcg
      481 ttgatttttc ctttattcat actgaaagaa atggagaaaa aagaaaaaat catctatgtt
      541 ggcgtagttt ctacatgggt atgcgtaact aatttatttc catggttttt gtttcaaaaa
      601 acaccgataa gcttactcca gtttccttgg cgtatcttag gtatccaaat ctttttttct
      661 tcaatgatca taacgcttgt ttttatgaaa aagactatga ataaaaaata ttcatggtta
      721 atcattggcg ctactgtggt gtttctactc gtcacgtcta tagcagcaaa agaaaattac
      781 gcaaaagtta tccaatcaaa acacggtcat attgttatca atgaagaaac actcccacgt
      841 tatacaacat cagatatggg aggattatac gattacgccc caacagaagc gataaaagaa
      901 aggcctcact tagaaaaaca tgaagtaaaa gtaggagaat cgtgggaaaa agggaagtat
      961 aaagctgcag ataataacat tacttacaca gtggcaagca acaaaaagca aaaagtgaca
     1021 gtaccagtat atgcttattt aggtacacag gtaaaagtaa acggtaaagt cgtaaatgac
     1081 tcttataaaa aacatggctt agtgaatgtg gatgtaaatg ctggagaaaa taagatagaa
     1141 attacaacaa aatatactcc aactgctctc atggctttat ggatcagtat tctgacttat
     1201 tgtttagtgg tgtattcaag ttttaaacga tttgcaccta ataagttgaa agattctcga
     1261 tatgtgacta aaaaggagat aaacaagaag aatgaaaata aataaaaaag tagtaatatt
     1321 gagtggatta attatagtta gtagtatata ttctttcatc tttttcaaaa atacacagag
     1381 tatttatacc actggagatc tttcttttca cttatctcga atcaaggggt tatcgactat
     1441 ttttaccagt ccgattaatt atgagacatt caattatact ggatatggtg taaattattt
     1501 ttatcccttt ctgacttttt ttccagctgt aatgttatat tggatgacaa aaaatttgat
     1561 tgtaagctat attatttacg tttggttatt gaacttgtgt acaacactcg tagcttatca
     1621 ttatggcgag cggtttttaa aacaaaaaaa agcggcattt ttatttagtt gtctctatat
     1681 tttctctgct tatcggacag tagacatcta ctatcgttcc gcaattgccg aagctatcgc
     1741 aataacactt gttatccctg tattatttta tgcttatcaa atcatctctg gaaaagaaga
     1801 aaagtatccg tctgtaaagc tagcattgag tatgagtttg ctagtgtaca gccatgtact
     1861 atcaacatta atgagtacag cattaattat catttttatt tttattcgtt tggtttctaa
     1921 aggtttcaag aatgcagatt ttattgctat atttaaaaaa ttatttagtg cggcaggaat
     1981 gacattggtt ctgacgagtg ctttttggta tccgatgttt gaacaaatgc tctaccaaaa
     2041 aatcaacaaa cctagcgtga caaatttata tgctcatgca tctaatgtgt tcgatagtct
     2101 gactgaagct atgaacaacg acttaactac ttactcaatg ggactagttg gtctgttatc
     2161 tttatgtatt cccctgattt tatttaaaaa attaacaaac atagaaaaaa agatttatta
     2221 tggtacgtgt ttgacttggc tagctacaac tagtttggtg ccatggtatc ttctgcaaaa
     2281 tacgccagcc aaattgttac aatttccttg gcggatctta agcttgcaaa taatattctc
     2341 ttctctgata ttaacgatga tatttttcaa aaatagaaga tacaataaga caagagaact
     2401 attttattta tttatgactg ttttgttatt aatcacttta actgtttcta gtaaaaaaaa
     2461 tttcaatcaa aaaataattt ctcagccggg acatattgtt gtaaacaaag aaacagtaga
     2521 ggcgtatacc actaaaggaa tggattattt tttatatgat tattctccta tcgcttcatt
     2581 ggagaataag gagcatataa taaaacacga agcaaagatt ggcagtcaat gggtaaacct
     2641 tccttataaa acaactggta gttctatcat atatagagta actaatgaga agagccaagt
     2701 aatcacactt cctgtatatg cttatttagg aacgaatgta gaaataaatc atcaacctgt
     2761 aaaggagcag gtgaatgcta atggattggt acaaataagc ggagtcaaag gagttgctga
     2821 tattaaagta agcacaagtt atacagtaac gactattatt tcttatcttg ttacattaac
     2881 tgcatattta tgtttatttt ttaaaatagt tttccataaa aggtaaatta acttttcatt
     2941 tataaaataa attgattaaa aaggagaatc tgtcgataag gattctctct tttttatacg
     3001 aaaaatctgt agggggatga agtaatcgct tatttcctca ctttatgata aattacagct
     3061 aaagtcacta gcttgtcttc ttcggaaagg aggagaatct atggatgagg aacaaatatt
     3121 cattcaaaca ctttataatc tgattttgaa tccgaataca cgagattggg aacgaaaagt
     3181 attgattcag acaaagaatg acataagaga aaatatatcc gttaaagaac agttatcaaa
     3241 actagaagca acactacgtc cattagctat ccgaatgaat ctgacgcctg atgtaatgga
     3301 cttttattta ttgattacgg aaggatttga taaggagcaa aaatatgact tttccaagca
     3361 tgccatgcaa gatgctgact atcaagaacg tgcagtattt gcagggggat gtttctggtg
     3421 tatggtcgag ccgtttgaat cgaaaaaagg gatcctatct gttttgtcag gatacacggg
     3481 cggacatgtt gaaaaaccaa attatgatca agtaagtggt ggttacacag gtcatgtgga
     3541 agctgtggag atcatttatg acacaagaga aataagttat agcgagttgc tgacaatcta
     3601 ttggcaaatt actgatccca cagatacatt tggacaattt caagatagag ggaagcaata
     3661 tcgtccagtg attttttatc aagtcgaacg acaaaaagaa ttagcggaac aatcaaaaca
     3721 gaaactagac agttcaggaa ctttccacca accaatcgtc acaaaaatcg aaccagccgg
     3781 tacgttttgg ccagcagaaa actatcatca gcaattttac aaaaaacaac cgaaacgtta
     3841 taaaaaaatc cagcaagcac gtaatcaatt tctgatctat caacgagtaa aaaacaaatg
     3901 gcaaaaaaat atacgaaaaa atcattttga ttaactcctt ttctttaaaa tgtagtaaac
     3961 taaaaataga aaaaagaaag cgttttaaaa agatttgatg aggaggagaa aaattgtata
     4021 ttgcagatag tgcacgttat gaaaaaatgg aatatcgtaa atccggtcat tcaggtttga
     4081 aattaccgat tttatctctc ggattatggc aaaattttgg tgattatgat cctatccata
     4141 atcaaagaga gattttaaga ggcgcattcg acatgggaat tacccatttc gaccttgcga
     4201 ataattatgg aggccctgct ggagctgcag agaaaaactt tgggcgtatt tttagagaag
     4261 attttcaagc ctaccgtgat gaactgatta tttctagcaa agcaggttat catatgtggg
     4321 aagggccata tggggaatgg ggctctcgta aatccatcat ttcaagttgt gaccaaagtc
     4381 tccaaagaat ggggctagat tatgttgaca tcttctatca tcatcgccca gaccctgata
     4441 cacctttgga agaaacagca gaagctttga tgcaacttgt aagacaagga aaagcattat
     4501 atattggtat atccaactat aatggagaag atacaaaaaa aatgactgaa attttaaaaa
     4561 gaaaggaagc accgttcatt attcatcaga tgcgctacaa tatgttttct cgtgcactac
     4621 tagaagacga tctatctcca gtattagaag aagaagggct aggggcaatc acgttcagtc
     4681 cgcttgctca agggttgctg acaaatcgtt atctacatgg tattcctgaa gattctagag
     4741 cacatcgtaa ggaaatacca ttcctttcag aagaacaagt tggatcaact ctggaaaaaa
     4801 tcaaagcact gcaaacaatc gccgtatcac gaggacaatc attagcgcaa atggcccttg
     4861 catggaattt gcgacaaaaa tctgttacaa gtgtactggt cggagcaagt cggttaagtc
     4921 agttacaaga aagtgttcga atgatggata atttagattt ctcaccagaa gaagaattga
     4981 gaatcgacca aattctagaa tagaaacgat gtaatcaaac tttttatcca tatattaaaa
     5041 aaaacaaaag atgacatcaa gaatcgaatg aagatcgtct gttattgaac atcatatttt
     5101 ttgtcgttca aaataaaaat gatcgaaggc taggagtgaa ttttatccta gtctttttta
     5161 tggtaaatat cttcttcgtt tgtatgtcta tgaacggatt aatcaatttt gtatacaaaa
     5221 aatcatcaga tgattcaaac gtgttataat aattacgaaa aaatacatca gatgaaaagg
     5281 aggtatgtgg tttggaagaa aataaaaccc tagtagaagt aactactgac aagatcctta
     5341 gatatttaaa ggaaatgcag ttgcaagtag gagataaact gccgaatgaa tatgaattgt
     5401 ccgattattt agaggtgggg cgaagcacgg tgcgagaagc tgtgcgggct ctagcttcca
     5461 ggaatatcct tgaagttcgg caaggagcgg ggacatttgt cagtgataag cgagggattg
     5521 ccaatgaccc actcggtttt tctttgattg aagatcagga tcggatgata cgagacttat
     5581 ttgaactgag atatttattg gagcctaaga tggcaggttt ggcagcggaa tatgcaacga
     5641 agaatcaaat cgtccaaatg aaaaaattaa cgcaaaagat tgaaacttct tttcgagaag
     5701 gcacacaaga acatgttcaa ttagacatcg attttcatac gatgattgca gaagctagcg
     5761 gaaacgtagc cttgctgcat attatcccaa tcattaatga atcaattgct ttgtttaata
     5821 aaaattatga tatccagcat ctgaaaatcg agacgatcga tattcataca gagatcgttc
     5881 aggccatcga aaggcggaat gcacttgctg cgtttgatgc aatgactgtc catatggcaa
     5941 taaatcggag agaattacag aaaataatag gagaaaaaga aactaattga cagttcatct
     6001 gatgtatttt atgataaata catcagatga atttttgttt tatatttggt aaaaaagagg
     6061 agtaaatagt ttctgttcat cgaaatgtaa gcgctgacta atagacaaaa agagatgacg
     6121 tcaaggagaa gaagcaggaa tgaggggatc tggaatgagt tttatggata aattagccaa
     6181 tgttttaggg aacgtggcaa cgagaatcaa taatttaagg tatatcatgg tgataaaaaa
     6241 cgcatttgct gcattgattc cggtaattat tacgggtgct tttggcacat tgttttcagc
     6301 aatggtcttt gactcggaaa atggcttagc acagataagt ttcttgcgtt ttttagaaga
     6361 attaaagcct atcgcatctg ccatcagcta tgtcacacta agtttcttga ccatctatgc
     6421 tgtttttctg atagggatcg aattagccaa gctgaataaa gtggatggtg tattccctgg
     6481 tatcatcgca gtgatgtctt atttatctgt taatccaact gtttatgaat tagtgaatga
     6541 aaatcagact gttatagtag aaaacgtatt ggctaaacaa tatacagata cgaaagggtt
     6601 atttttggga atgttggtct ccatcctttc tattgaactt tattgctggt tgaggaaaca
     6661 gaagaaacta cagctgagaa tgcctgatac cgtgccaacg aatgtttctg ctagtttttc
     6721 tgctttattc ccgacgattc tgactgtatc gggtatcgcg gctgcgggat ttatcatcaa
     6781 agaacttaca ggaatgtacc tttatgatat catttacaat gtcgtacaac gcccattaga
     6841 aggtgtcgta caagggttac cgggtatttt gctattgatg tttattgcgc aagtcttttg
     6901 ggtgattggt atccatggaa atcaaatggt gaagccgata cgggaaccgt tgcttttagc
     6961 ggcaattgct gtaaatacgg aagcttttga agctggaaaa gaagtaccga acattattac
     7021 tatgccattt tgggatatgt atatgagtat gggcgggtct ggagtcacga tcggtttact
     7081 gattgcagta ttgttagttg gaaaacggga agatatgaaa caaatcacca agttatccct
     7141 tgctcctggg atcttcaaca tcaatgaacc agttattttt ggtatgccga tcatgctgaa
     7201 tccaatttta gctattccgt ttatcttaac gcctcttatc actggaacga ttggttattt
     7261 tgcaaccgct ttaggatttg cggcgaaagc agttgtaatg gtcccttggc caatgccgcc
     7321 gatcgtcaat gcttatctgg caacagcagg aaatatcggt gcagttatta cgcaacttgt
     7381 ttgtattatt gtttctattt tgatctatct gccatttgta aaagtagcaa atcgtgtgga
     7441 aaatgatggg atctaaaaaa caggaggaaa tcatgaaaat tactattcca aaaaatttta
     7501 ttcttggtgc tgcatcttct gcttggcaaa cggaaggttg gaaaggaaaa aaagaaggac
     7561 aggattcgtt tatagatagc tggtataaaa atgaacattt tgtttggcat aatgggtatg
     7621 gcccagccgt agcaactaat tttatggaac aataccagca ggatgtggat ctgatgaaat
     7681 ccatccatct gacacattat cggacttcta tcaattgggc tcgttttttt actgattatg
     7741 agaactgtat agtagatgaa gaatatgctc agcatataag tgaaatgatc gactctttga
     7801 tcgaagcaga cgtagaaccg atgatttgtt tggaacatta tgaagtccca gcttttcttt
     7861 tggaaaaata tgatggctgg tcttcaagaa aagtagttga tttttatgct aagtatgctc
     7921 aaattgcctt tgatcgttac ggtgataggg taaaaaactg gtttacattc aacgaaccaa
     7981 tcgttcccca aacgcgttgc tatttggatg ctgttcgctg gccgcatgaa caaaacacaa
     8041 aaaagtggat gatgtggaat tatcataaag ttttagcaac ggctaaagca gtagagattt
     8101 accgcagcgg aaattatggt ggaaggattg gctgtatttt aaatcctgaa atggtctatg
     8161 ctcgatccaa ttctccagaa gaccaaaaag ctgcttatat gtatgatttg ttttataatc
     8221 gagtattttt tgatccgatg gtaaaaggat actacacaga agagttcctg gagttgtgtc
     8281 aaaaacaatc gatttatttc gatcctgcaa cagaggattt agaaacgatt cagcatcata
     8341 cagtagattt ccttggaatc aatcaatatt atcctaaacg cgttcgcgcc cctcgttatc
     8401 aatggaatga agagacacct ttccatcctg aacgatatta tgaagtcttt gatttacctg
     8461 ggaagaaaat gaataattcc aggggatggg aaatctatcc gaaaatcatg tatgatatag
     8521 ccatgtatct aaaagaaaat tatcataata tcccttggct gataacagaa aacggtatgg
     8581 gaagagaata tgaagaacag tatatggatg agaaaggaat cgtgcaagat gattaccgta
     8641 tcgaatttat cggacagcat ttgcattact tgatcaaagc aacagaagaa ggcgcaaatt
     8701 gcgaaggcta tatgttatgg gcattcactg actgtgtgtc accaatgaat gcatttaaga
     8761 atcgatatgg attagttcga atcgaattga acgatgaacg aagcagaaca ttgaaaaaat
     8821 ctgcttcgtg gtatcagact ttgattgata agagagaatt ggatttgcct gacgaaatct
     8881 ataaataaat agaagttttt tgtagatgtt cattgtattt actggttatt tgaaataaaa
     8941 gaggttgtga atccagcgtt ttgagctaat acccagggtc agctgattga acaccgttta
     9001 ttgagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaatagcaaa gtaaaaacaa
     9061 catcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaataccgt
     9121 ttatttgaaa aaagttgtga caagttttgt gtcacaactt tttttgtgca cgttaaaaga
     9181 aaaaaggtaa taaaaattaa tttgtgtgtt tagtgtgcgc tttctttttt agtgtgtatg
     9241 caacttggta tagaccttaa atgtgatgat atcaactttt taaagcgctt ttaaagttgg
     9301 cacgctactt gcttatatac aagtgtaagt atagaaagga acgtgtttaa aatggaaaag
     9361 aaaacaatca tgcttgtatg ttcagctgga atgagtacaa gtctcctagt cacaaaaatg
     9421 caaaaggcag cagaagcaaa aggaatggaa gcagacatct ttgctgtatc tgcttcagat
     9481 gcggataatc aactagaatc taaaaatgta gacgttttac ttttaggacc gcaagtacgc
     9541 tttatgcaaa atcaatttgc tgaaaagcta gcacctaaag gcattccttt agatgtaatc
     9601 aatatggctg actatggcat gatgaatggt gaaaaagtac tcgaccaagc agaaaaacta
     9661 ataaacaaat aataaaaatt atcggaggat acaataaatg gaagatcaaa aaaatctaga
     9721 agccatcatg ggtctgatca tgtttggcgg aaatgcaaaa agcgatgcaa tggaagcgat
     9781 tgctgctgcg aaaaaaggtg atttcacttt agcagatgaa aaaatcaaag atgcagagga
     9841 atctttagtc caagcacatc actctcaaac agagatgctg acacaagaag cacaaggaaa
     9901 tcacgttgaa gtcacgcttt tgacggtcca cagtcaagat catctgatga catctatagc
     9961 ttttacagat ttagcaaaag agatcattga tctgtatcgt cgattgaacg cgtaatctac
    10021 agtaaagaaa agagggaaga tttatggata aatttgttgc atttatggag aaacatttta
    10081 tacctgtagc atcaaaaatt ggtgcacaac ggcatttagt tgcgattcgg gactcattta
    10141 tggtgagtat gccgttaatg atcttaggag cattagcagt aatgatcaat aacttaccga
    10201 ttcccgggtt ccaagaattg atgaactcaa tttttggtgg tgaatcatgg aaaggattcg
    10261 gggctgctgc ttggaacggt acatttgcca ttctttctgt cttgattgct ttcttgcttg
    10321 cttatcattt agctaacggc taccgaaaag acggtgtttc cgcaggtgtg atttctttag
    10381 gctctttctt cgctttaggc ggagcattag gtatgagctc aaacggtttg tttattgcta
    10441 ttattgttgg gattatttca acagaaatct ttgtacgttt aagcggaaat gaaaaattga
    10501 tcatcaaaat gcctgatggt gtgccaccgg cagttgcaaa agcatttgct tcattactac
    10561 cagcaatgat cactatctct gcgtttgctt tagttgctgc aatttttgct gcattcggtg
    10621 ttgatgatat cgtaggttct ttctatacag tggtccaaga accatttatg ggacttgcaa
    10681 actcttatcc atctgcctta ctgcttgcat ttatcacacc attcttatgg ttctttggtt
    10741 tacacggagc gaatatggtc gatcctttga tgcaaacaat caatgcgcca gccattgaag
    10801 caaacgttaa tgcaatcgca gctggacata gcgcaccgtt tatcgtgaac aaacctttct
    10861 ttgacagctt tgtcaactta ggaggaacag gtgcaacttt aggtttactg ttagctattt
    10921 atttagtagg acgtaaaaac aaaccatata tggttgtaac aaacttgtcg attgcgccag
    10981 gtgtcttcaa tattaatgaa ccaacgatgt ttgggttacc tatcgtgttg aacccgatca
    11041 tgttcattcc atttattttg acaccaatgg tcttagtt
//
LOCUS       DAFGFZ010000095        10760 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000095 DAFGFZ010000000
VERSION     DAFGFZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.097"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="LR726_002205"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="LR726_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="LR726_002206"
     CDS             complement(1437..2279)
                     /locus_tag="LR726_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL6217253.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="LR726_002207"
     CDS             2604..3020
                     /locus_tag="LR726_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL6217254.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="LR726_002208"
     CDS             3017..3487
                     /locus_tag="LR726_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217255.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="LR726_002209"
     CDS             3501..4274
                     /locus_tag="LR726_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217256.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="LR726_002210"
     CDS             4288..5100
                     /locus_tag="LR726_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL6217257.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="LR726_002211"
     CDS             5154..6119
                     /locus_tag="LR726_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL6217258.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="LR726_002212"
     CDS             6256..8979
                     /locus_tag="LR726_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL6217259.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="LR726_002213"
     CDS             8992..9624
                     /locus_tag="LR726_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL6217260.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="LR726_002214"
     CDS             9829..10056
                     /locus_tag="LR726_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBL6217261.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="LR726_002215"
     CDS             10125..10391
                     /locus_tag="LR726_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL6217262.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="LR726_002216"
     CDS             10381..10650
                     /locus_tag="LR726_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL6217263.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt
//
LOCUS       DAFGFZ010000096        10661 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000096 DAFGFZ010000000
VERSION     DAFGFZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.098"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..944
                     /locus_tag="LR726_002217"
     CDS             <1..944
                     /locus_tag="LR726_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL6217264.1"
                     /translation="GSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQIEKGFGQTMT
                     AEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGINTVLETSFCG
                     AYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIEYPNCEVRIRV
                     PVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLEYPLADTPAPS
                     PKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAASSRIELLLKS
                     VSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1022..1258)
                     /locus_tag="LR726_002218"
     CDS             complement(1022..1258)
                     /locus_tag="LR726_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL6217265.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1227..1358)
                     /locus_tag="LR726_002219"
     CDS             complement(1227..1358)
                     /locus_tag="LR726_002219"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL6217266.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1434..1817)
                     /locus_tag="LR726_002220"
     CDS             complement(1434..1817)
                     /locus_tag="LR726_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL6217267.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(1850..2635)
                     /locus_tag="LR726_002221"
     CDS             complement(1850..2635)
                     /locus_tag="LR726_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL6217268.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            2813..3913
                     /locus_tag="LR726_002222"
     CDS             2813..3913
                     /locus_tag="LR726_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL6217269.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            3903..4727
                     /locus_tag="LR726_002223"
     CDS             3903..4727
                     /locus_tag="LR726_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL6217270.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            4724..5539
                     /locus_tag="LR726_002224"
     CDS             4724..5539
                     /locus_tag="LR726_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL6217271.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5536..6366
                     /locus_tag="LR726_002225"
     CDS             5536..6366
                     /locus_tag="LR726_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6217272.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6373..7404
                     /locus_tag="LR726_002226"
     CDS             6373..7404
                     /locus_tag="LR726_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217273.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7511..8784)
                     /locus_tag="LR726_002227"
                     /pseudo
     CDS             complement(7511..8784)
                     /locus_tag="LR726_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9061..9270
                     /locus_tag="LR726_002228"
     CDS             9061..9270
                     /locus_tag="LR726_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217274.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9320..10036
                     /locus_tag="LR726_002229"
     CDS             9320..10036
                     /locus_tag="LR726_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL6217275.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(9938..10458)
                     /locus_tag="LR726_002230"
                     /pseudo
     CDS             complement(9938..10458)
                     /locus_tag="LR726_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2954 a         2066 c         2493 g         3148 t
ORIGIN      
        1 agggttctct acatgatggt gctggtttac ggaccgttgt ttatttaaag ggctgcccat
       61 tacgctgtgc ctggtgttcg attcccgaat cacaaagcaa gcagattgaa aaaggatttg
      121 gccagaccat gaccgctgaa gaggtaatgg atgagattga gaaggatgct gttttttact
      181 ttcactcgga tggcggagtg accattagcg gaggcgaggc actggttcag gcggattttg
      241 ctaaagaaat tttgcaaaaa tcgaaataca ttggaatcaa taccgtactg gaaaccagct
      301 tttgtggtgc ttataatgaa atccaaaagg tagctcccta tgtggataca ttgtttgtgg
      361 atgtaaaaat gtttaccagc aagctccaca aacaatggac cggcttagat aaccaacaaa
      421 ttttgaagaa tattcgtcgc tttttaattg agtatcctaa ctgcgaagtg cgaattcgag
      481 ttcctgtggt tccaggtatc aatatgaacc tgaccgagct gctgacgatt gcttgttttg
      541 tggcagatct tgatcggttc gtaccgttag aattactacc gtaccactgc tatggtatgc
      601 acggttacca agcattggga ctggagtatc cgttggcaga cacgccagcg ccttcaccta
      661 aagaaatgtt tgcattggca gatcaactgg ctcgcacggt tcctcatttg ctggtcacag
      721 ctttaaccaa aacatttatt tatttattga atccaagctt tatcttatgg ataaatgaaa
      781 gacactcact agctgcagcc agctcaagaa tagagctttt gctgaagtca gtgagtgtct
      841 ttttacgatg ttttttcgaa gttaatttgt cgaatgaaca ctggctcaat cggtacatct
      901 tcactgtttg tcggctgagc tgcgatcgct gctacgattt ctaacccttg gtatacctgt
      961 ccaaaaacag tataacgacc atccagctcc ggtagtcctc caagctccaa gtatgcttgc
     1021 tttacattag caggtcagcc tgcgcctttg tgttttgcac aataaaaaac tggttgccgt
     1081 tggtgtgggg gccgtggtcc cctaaggcca aggcgccatt aaaatgataa agtccgcgat
     1141 gcacttcatc agcgaaaaag ccctcatagc tggaaacagc ttcatcacca gaaccatcca
     1201 aagcgccacc ttgaataaca aattccttaa tgacacgagc cgaaaggtgt gttgtcataa
     1261 acgccttgct cagctaggga gcgccagttg gctacagcca ggggagcttg gtcaggaaat
     1321 aagcggagac gaatttcacc agcatcggtg accagcacac aaacttcttc ctgctgcggt
     1381 ttttctaatt gaaagagagg aaggtttgca actgaaacgc cttgatcctg ccatcaaata
     1441 tcgaaaatcc gccactcgac cttcgcctga tcaccaaggc gagccattcc caataaaacg
     1501 gtataggcgg tttcatccgc ttgaaaggtc aattgcagct gtcctcgtaa atcgttgtag
     1561 tcatctgggg cggctacttg ataaaaaaga tgaaactgcg catgcaaata ttccaaaggc
     1621 tgatctttaa aggctgcaaa ataagcctga actttttccc gctgcaagct gtcagccacc
     1681 aaaaaatttt ccagttgttg ttttggaacg gctgctaaca aggatcggca aaccgcctca
     1741 attttcaacg catcccaatg tcgcgcaatt aaataatgga tgggtgcctc ttgattgtag
     1801 tcccacaggt tgctcatttt tttcaccact ttcttctatt atataggaat cacaaaatcg
     1861 gcagcggcgt caggcggcgt tcttttcctt gaatgactag caacttttct acctgacagt
     1921 ctgccaacag ctgactcatt tttttaaagc ccagctgata atcttcggca acatgagcat
     1981 ccgagcctaa ggtaaagagc tttccgccga cactttgata caaaggaacg gcataacggt
     2041 aaaggtcttc gttgccgtat ttcaagaagc tcttggcatt taactccatc gccaattctc
     2101 gttcaacaac ctttttgaaa atcgttagca gctgagcctc aaaatgctcc gcgaattctt
     2161 ccgctgtgaa ggtgaagcgg cgtaatccat aatcaaagtg agtcaaaata tctccttctg
     2221 gaaaatccgt caagacctgc aacatctgat caaaatattc cttggtagtt tgaatgggat
     2281 ctttggttaa aaccacatcg tccatataat caaagcggcc attttgatga atacttacca
     2341 gctttaaatc ataaggatgc tgagcgaggt aatccacgat ccgctcttct tgtcctggaa
     2401 caacgccaat ctcgatacct cgcagaaatt tggtggaata ggtttctttt aaagagtcca
     2461 gtttgtttat ataggctgga taatccggaa tgtcatcttt tccggtagat ggattgttta
     2521 aatcaaagtg atccgtagaa acgaaaaact ccggctgata ggctaaatag ttttcaaatt
     2581 tttcctctga atcaaaggaa aaatacgtgt ggagatgctg atcataatac tgcataggga
     2641 gcctcctttt ttctactatc ataccgcaaa agctttagaa ggttaacggg aattcgtcga
     2701 aatttacaaa ccatatacaa cctttacatt gttcatgaaa ccttttcaag aaactgattc
     2761 tctcattaca aagctttgtt agaatggaat tatcgaaagg ggagaagaat gaatgaaaat
     2821 gaatgaaaaa attcgtaaca tgactatcgg tttattttgt gcagcaacag taagtatcct
     2881 gctgacaggc tgcggctcag gtgaagcaac gaagcaatcg gctgcggcag acgactcttt
     2941 agatacgatt atagaaaaag ccaaggaaga aggagagatt gccagcttgg gaatgccgga
     3001 tacttgggca aattgggtag gcacttggaa cgaccttgaa aaagaatacg gcttaagtca
     3061 cgtggatacg gatatgtcca gtgcagaaga attggctaag tttgaatcag aaggaaaaaa
     3121 tggaaccgcc gatattggtg atgtcggtat cagcttcggt ccgttggctg aaagcaaaga
     3181 gttaacctta ccttataaga ccaactattg ggaggaaatt ccggattggg caaaggatga
     3241 tgatggggat tggttgttaa gctatacggg aaccatttcc tttatcaccg ataaagagaa
     3301 tgtgaagcaa gcaccgacta gctggaagga gctgcaagag ggtgactatc aagttagcgt
     3361 gggggatgtg acgattgcca accaagcaca atttgctgta ttggctgcgg cgattgccaa
     3421 cggaggtgac gaaaaggata ttcagccagg tctgaattac tacaaaaaaa tggcagaaaa
     3481 aggccgtttg tccaccgttg atgccagcgt agcaaatctt gaaaaaggtg aaatcgatgt
     3541 ggcagttgtt tgggacttca atggactaaa ttatcgtgac caaattgact ccgagcgttt
     3601 tgaggtaact gtgccaacag acggctccat cattagcggg tatacaacct taatcaataa
     3661 aaatgcccct catcccaatg cggcaaagct agcacgggaa tatattttat ctgatgaagg
     3721 gcaaatcaat ttggcaaagg gctatgcgcg acctattcgc aaggtagagc tgcctaaaga
     3781 agtagctgaa aaattactgc cggaggatgc ttatgaagcg gcggtgccag ttgaagacaa
     3841 tgaagcttgg aacaagacct ctgtagagct tccagagctt tggcaggcgg aggtactgac
     3901 ctatgcaaag tagacttgac aaactgattg tagttatact agacggctgt cgctacgaca
     3961 cagccgtcgc ccagcttggg ttcatgaatc atttagttga gcaccagcaa gcaagcttat
     4021 tgaaggtaaa aagtgagctg cccagcaatt cacgtccgtt atacgaagta ctgatgaccg
     4081 gcgttccagc ctatgaaaat ggcatctata gtaattacca tgctcagcga tccaaagagt
     4141 ggtcgctgtt tgaattagtt aaggaagctg gcggaaaaac aggagccgcc gcttaccact
     4201 ggtacagcga gttgtacaat caagccccct ataatcgttt ggttgatcgc atccagcatc
     4261 aggaggagcg actgattcag catggcatct tttatagtga ggacacttac cctgattcgc
     4321 atttatttgc ggatgcccat tatttagtga atcagtatca gccagacttt ttggtcgtac
     4381 attcgatgaa tattgatgat gtcggtcaca aatttaccgc cgattctcat gaatatgcag
     4441 cagcggttaa tcaggcggac agattattgg ctatttacct tcctgaatgg ctgcaaaaag
     4501 gttatcaagt cgtggtgact gcggatcacg ggatggatgc gcacggactg catggcggca
     4561 gcttggctgc acatcgtgaa gttccttttt atttgatttc aaataaacgc caaaagctga
     4621 aggaacaaac tgtaccacag ctgctggcgg ctccattgtt ttgctggtta ttaggaattg
     4681 caccgtcaga gaaaatgcag gacattcaag aattgatgag gggatgaaag agatgaaaaa
     4741 acgcttggtt ctggttcctg cctcctttat cttctgtgca tttttactgc tgccgctgct
     4801 ttttatggtt ctgtgcagct tgcacagtga tttaactaac cagtggacat tggccaatta
     4861 cagtgatatt ttcagcaaca aatattacac acaggccttt gttaacagcg tactgattgc
     4921 tgtcagctcc agtgtgattg ggttaatcgg gacgtttatt ttatgtttat gcttaatgaa
     4981 tctatcgact aaattgcagg agaaaatcac ctttgtttcc aatttggcag ctaattttgc
     5041 tgggattccg ctggcattct cttttattat tttgttgggg aatgtaggtg tgctgaaaat
     5101 gattttgcca tttctagcgg acttcaattt gtattcttgg tggggattga ccatcacgta
     5161 tacctatttc caaattccgt taggggtctt gtttttgtat cccagcatga aggaagtgaa
     5221 aaaggaatgg ctggagtccg cacaattgct gggcgcttca tcagcgtatg cttggttcaa
     5281 ggtggctttg ccttttttgc gaccatcgat tgccagcacc tttgtcatct tatttgctaa
     5341 tggcatgggg acctatgaaa cagcgtatgc cctgaccagc agtaatgtaa atcttttaac
     5401 gattcgaatt gcggctttag tgtcgggaga tgtgttcgcc aagcctaatt taggcagtgc
     5461 gttggcagtc gctttaggtg tcctgctggt aacggcaatg ttgattattc aaaagaaagg
     5521 caaggtggtg cagacgtgaa aaagttaata aaaccgattt tgttctgctt tgtcctgtat
     5581 tacttgctgc cgattattgg aacgattttg tatgccagct ccactaaatg gtcaaaatcg
     5641 cttttaccta gtgattttac cttgcaatgg ttccaacagc tgctgacgga cagggaattt
     5701 attgctgcgg tgggtcgctc acttttatta gcaggtgtgg tcttggtgac gattctgttg
     5761 ttgatgattc ccaccattat ttggattcat ttgtatttcc ctcgtttgaa tcgttggctg
     5821 gaaaagctgc tcctgctgcc gtatgcttta ccaggagtga ttttggtaac ggctttgctt
     5881 cgaacgtatg ctgaaactgg tatcccaatg tttgtagtgt tggtaggtgc attgtttatt
     5941 acggcgttgc cgattgtcta tcttagtctt aacaaccaga tgcgcttaat taatctgaag
     6001 gaattagtgg atgcagcaga aaccttgggc gccccgatgt ctacgattat tatccaggtg
     6061 ctgtttccaa acatccgtat cggtgtgacc ctagtttctc tgatgatttt ttcttcggtt
     6121 tttggggagt acatgctgac gaacctgttg attggcggtc gatttcagac ggtacgcatc
     6181 tatatgctgc ggcggatgaa cgaaaacggc catttggcta gcgctgtgat ggttttgtat
     6241 ttgctgttta tggtgggaat tgctttaact acctttatgc tgacttatcg acaaaagcaa
     6301 cagctgtccc ttaagcagac gagggaagag aagcagtcga aaaagaagcc cttaaaggag
     6361 ttgtgacgga aaatgttttt agaaatacat gatctcaatc tgttttttga tgaaaaacag
     6421 attttagcta ttgatgaaat tcagattgaa aagggtgagc tggttacttt actaggtccc
     6481 agcgggtgcg gaaagtctac gttgctgcgc tgtatcacgg gattggaaca accaaagagc
     6541 ggtgagattg tgctggacaa tgagaatatt gccgataagc ctacgaagga tcgcaatatc
     6601 ggctttgttt ttcaacagta tgcattgttt ccaactatga ctgtttttga aaatgtagcc
     6661 tttggtttga aggtaaagaa gctgagtcct gaggtgattc aggaaaaggt ttttgagatg
     6721 ctatcattgg tggatatgac ggagcaggca gataaaaatg tccagtttct ttctggcggt
     6781 caacgacaac gggtggcact ggctcgctca ttggtcactg aaccgaaagt cctgctgctg
     6841 gatgagccat taagtgcttt ggatgcccga attagaaaac agctgcagcg ggatttgcgc
     6901 gctatccagc aatcgctagg gatgacgatg atttttgtga ctcatgacca agaagaggcg
     6961 atgcgaatca gtgatcggat ttttgtgatg gaagctgggc gggtggctca ggtttccacc
     7021 tcgaaggaat tgtatcagaa tcctcagagc cgatttgttg cggaatttat cggcaactat
     7081 aatcgtttcg aatggtatga gctgaatcag tatacccacg agttttccgc taagggcaat
     7141 cgctgcattt attatttaag accggagctg attcaatttg aaccggttcc tcagggggtt
     7201 aagattccgg ttctatggaa ggaatcattt attttaggca acattcaacg gtatgttttt
     7261 caaacgctgg agggcaaaga aattttagtg gatcggttga acgatcggga agagaaaatt
     7321 agcgatgcct tatacattca gctgaaagat attctagcaa tccctgtgga aattgatact
     7381 cagcattttg cacaacaaag ctagtgtata gtcggcaaac atttctggct ctttgtcaaa
     7441 taggactgat agagtgatat aagaacatct aaggtagaat tgattttctg ctttagatgt
     7501 ttttttatgt ttaataagtg atcagatgat tgattaagaa aattctggtt ctcatcctaa
     7561 cccgtaagct attcgtttca atgctttgat gtgggtatgc agattttcta ttttgccatt
     7621 tgagtaaggc aactctaagg ctaaacgaat gccggattca tagtttataa acgctttttt
     7681 tatatgatgc agttctcttg gaaccgactt gggcattgtt tttacaagct gaaagaagat
     7741 gtccgcttct ttgtcataaa aagcttgaag aatttcttga tagtaagcat aagctattcg
     7801 tagttcatca gaagtagaaa gtagacgttc tacgacttca ctttcggtta aataggccca
     7861 accaaaatta cgccattttg tccatttatc ataaacaagc gtgtcctgtc gtttgatcag
     7921 aagtcgagat aaagccttca cttgtcgata gtgtttggct tgtattggat cgtttcggtc
     7981 gaagcttttc atgactcata gtcaaatatc cttaaaactg cgaatgaggt gcttgacgat
     8041 gtgaaagcga tccacaacga gtttggcatg aggaaagcac tcttttatca gcaaagcata
     8101 agaggcgttc atatctgtta cgagtatttt tacgtttaaa cgtgcacgtc gttcaaattg
     8161 cataaaataa gaaatgagat ctttcttctt acgtgaagga agaatatcca gtaatttccc
     8221 agtatcgcca tccatacaag aaaaagaata gggaccgact tgatttttca atgaacgaaa
     8281 ttcatctacc aatgaacagc gaggcaacca atttattttc acgtttgtca tggggagcag
     8341 agaagcgagt gttcgcgaaa cggttgacca agaaacgccg caatgtttag aaatcgtcgt
     8401 cattgcttgt ttttcagtca attcctccaa tattttataa tgtacttgtt tggcgataaa
     8461 acagtttttt tcaacaatgt aggtattcgc cgtaaaatag gtgtgacagc cacgacagag
     8521 gtaccgttgc tttgccaaag caagataagt ccgttgattc gcacacttat tgagaaggac
     8581 cagcgatttt ttctttccgt tcttaacgat cagcccatgt tgattcacct gtttacactg
     8641 aggacagggc tgaatcgttt taatgagcgt ggcagaaaca acgatactgt tgattgaatg
     8701 tatttttttc ttctcgacat gagtgatttg aatgtttcca tctttgattc ctagtaatag
     8761 tttgataaga tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa
     8821 ttctaacgga gcttctgtat gttttgtttt tttatgctca aaaggactga tagaatgatt
     8881 tactatcagt cctagaaatt atagcgccac atttctttca cctttgactt ttcagacggc
     8941 agtttttatg ctgatactgt agagagagca ggacacagct tttgcttgtt ttgggtaagg
     9001 gggaaagaag atgaccaaag cacttatttt tgattttgat gggacgatga ttgacagtga
     9061 atggatgttt tacgagagta ttcatcacta tttactgaag cctatttact ggagcagttc
     9121 ggctataaaa ttccttttgc agactatcaa caaggtgttg ggacggcagc agaactgtcc
     9181 ttttatgaag ggataagtca agcggttggg agaccagtgg atgctgaaca ttttgagcag
     9241 cggatcagca gcactggaaa atcggagtag tctcaattct gaactgcggg agctagacta
     9301 tttttttgaa gcacatccga tgattaagga aaaaattgat tcctttgtga cgatcgatga
     9361 tcttcaggtc ggcaaaccag atccagaagg ttatttgttg tgcttgaaac ggctgagggt
     9421 tgaacaggct gttgtagttg aggatagtcc agtcggtgcc caagcagcac cgcatatgac
     9481 agttgatgta caagcgttgg atgcagattt tttcgccttt agcgggcata aaatgtgcgg
     9541 gccgacagga atcggtgtat tatatggcaa acgtcaattg ttagaacaaa tggagccagt
     9601 cgaattcggc ggtgaaatga tagattttgt caatctttac gatagttcat ggaaagagct
     9661 gccatggaaa tttgaagctg gtactccgaa cattgcagga gcaatcgatt acttgaatga
     9721 gattggtatg gagaatattc atcgttatga gcaagaactg gttgcctacg ttttacctaa
     9781 attacaagcg atcggcggta tcaccattta cggaccgcaa gatcctgaac atcatactgg
     9841 cgtggttgct tttaatttgg atggcatcca tccccgtgat gtggcgactg ctctggatat
     9901 ggaaggtgtc gctgtacgtt caggacacca ttgtgcacaa cggttcatcg acgatttctg
     9961 tttgactaat tggtcgttta aacagtcgat gataatgccg tttgagcggc tccaatgtcg
    10021 tggaatcttt gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt
    10081 cagattacgt gattgatttt cgtacagttc tatgagaatc gcctgcttca gttcctttgt
    10141 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtctgcc cacaatcata
    10201 gcatagaaaa cgggtacgtt ttaattctaa aatagtcaac ttttctttga ctttcaacat
    10261 ctgagtacgt gtcgtatagg aaccgttgcg gataatacga atcgatccac agtgttcaca
    10321 atgggaaggt gagtcatcca atctcccttt aataaactgc gcttgaacgc ctttatattc
    10381 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
    10441 tctagtatga ttatccatag agcgactcct ttggtagggt gatgttttgg tcgactaaat
    10501 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
    10561 ccttcgattt tccaacaaca caaaatatta tagaagcctt ttttctaaag gatttcaagg
    10621 tgtctgtttg catttattaa aaaaaagaaa aagtattggc a
//
LOCUS       DAFGFZ010000097        10588 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000097 DAFGFZ010000000
VERSION     DAFGFZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.099"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            33..1094
                     /locus_tag="LR726_002231"
     CDS             33..1094
                     /locus_tag="LR726_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL6217276.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="LR726_002232"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="LR726_002232"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL6217277.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="LR726_002233"
     CDS             2007..3989
                     /locus_tag="LR726_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL6217278.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="LR726_002234"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="LR726_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL6217279.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4711..6135
                     /locus_tag="LR726_002235"
     CDS             4711..6135
                     /locus_tag="LR726_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL6217280.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6316..7089)
                     /locus_tag="LR726_002236"
     CDS             complement(6316..7089)
                     /locus_tag="LR726_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL6217281.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7086..7991)
                     /locus_tag="LR726_002237"
     CDS             complement(7086..7991)
                     /locus_tag="LR726_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6217282.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8145..8465)
                     /locus_tag="LR726_002238"
     CDS             complement(8145..8465)
                     /locus_tag="LR726_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL6217283.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            8939..9625
                     /locus_tag="LR726_002239"
     CDS             8939..9625
                     /locus_tag="LR726_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL6217284.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            9627..10571
                     /locus_tag="LR726_002240"
     CDS             9627..10571
                     /locus_tag="LR726_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL6217285.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
BASE COUNT         3273 a         1862 c         2302 g         3151 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     4621 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     4681 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     4741 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     4801 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     4861 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     4921 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     4981 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     5041 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     5101 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     5161 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     5221 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     5281 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     5341 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     5401 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     5461 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     5521 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     5581 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     5641 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     5701 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     5761 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     5821 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     5881 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     5941 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     6001 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     6061 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     6121 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     6181 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     6241 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     6301 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     6361 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     6421 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     6481 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     6541 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     6601 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
     6661 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     6721 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     6781 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     6841 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     6901 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     6961 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     7021 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     7081 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     7141 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     7201 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     7261 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     7321 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     7381 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     7441 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     7501 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     7561 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     7621 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     7681 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     7741 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     7801 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     7861 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     7921 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     7981 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     8041 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     8101 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     8161 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     8221 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     8281 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     8341 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     8401 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     8461 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     8521 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     8581 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     8641 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     8701 gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata
     8761 tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat aatttttgtg
     8821 ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa acaactatat
     8881 tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag gaaaatatat
     8941 ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt
     9001 agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg
     9061 cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat
     9121 ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc
     9181 ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg
     9241 gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt
     9301 tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca
     9361 tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt
     9421 ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa
     9481 agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat
     9541 cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa
     9601 atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag
     9661 gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag
     9721 taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg
     9781 tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga
     9841 tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc
     9901 catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt tttattccag
     9961 ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta ggtgctttag
    10021 tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt tctcgagata
    10081 atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta gggccgttaa
    10141 gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct ggagttggga
    10201 cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac atcggcatca
    10261 tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca tttgcggcca
    10321 tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga gcgaatccag
    10381 cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg
    10441 ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa tacggagtga
    10501 tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct tatatgattc
    10561 tatttaaata atagaagaga ggggttgt
//
LOCUS       DAFGFZ010000098        10346 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000098 DAFGFZ010000000
VERSION     DAFGFZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.100"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(72..650)
                     /locus_tag="LR726_002241"
     CDS             complement(72..650)
                     /locus_tag="LR726_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6217286.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(670..1338)
                     /locus_tag="LR726_002242"
     CDS             complement(670..1338)
                     /locus_tag="LR726_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217287.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1353..2423)
                     /locus_tag="LR726_002243"
     CDS             complement(1353..2423)
                     /locus_tag="LR726_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6217288.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2594..3958)
                     /locus_tag="LR726_002244"
     CDS             complement(2594..3958)
                     /locus_tag="LR726_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL6217289.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3955..5514)
                     /locus_tag="LR726_002245"
     CDS             complement(3955..5514)
                     /locus_tag="LR726_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217290.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5570..8050)
                     /locus_tag="LR726_002246"
     CDS             complement(5570..8050)
                     /locus_tag="LR726_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217291.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8034..8369)
                     /locus_tag="LR726_002247"
     CDS             complement(8034..8369)
                     /locus_tag="LR726_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217292.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8383..9417)
                     /locus_tag="LR726_002248"
     CDS             complement(8383..9417)
                     /locus_tag="LR726_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL6217293.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9507..10187)
                     /locus_tag="LR726_002249"
     CDS             complement(9507..10187)
                     /locus_tag="LR726_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL6217294.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2963 a         2112 c         1811 g         3460 t
ORIGIN      
        1 agaagcaaga tataacgatc ttcgtcatat cttgcttctt cacctaacta ttttctccct
       61 atataacagc tttacgcttc tcctaataca ccattcaaat ataaatcgat caattgcgcc
      121 acatttagcg gttcttccac aggtcttttc gaccgagtga tgtaaggtgc taacactaag
      181 aaaaaatgcc ttgctgcgat ttcttttgaa atcccatttc ttagcacaga ttggtttgct
      241 gaaaagattt cttcaaaagg ttcacgataa tttttcttcc atagaaagaa aagatgatct
      301 cttgtttcct ttgaaagatt gctttcaaga tcgtgaagca tcagaaacaa atcgaccaga
      361 tgtgtctcaa tcaaaaactg cgccattttt gtcaaggtta cttgaaatcc cgtttcgctt
      421 gtttgaacga tttctctcaa agcttttcca acatctttta agtggttttc taaaactgcc
      481 gtatatagtt tttctttatc accgaaataa tgatagagat tgggctgggt gatcccaact
      541 ttttgtgcga tttctcttgt tgaagtgccg agatatccct tggacataaa caagtcagtc
      601 gcttctttca aaattttttg ctgtgtatct ttgttgtatc gatggaccat ttttccacct
      661 gctttttggt tattcttttt ctttcaatac accatcttcc atcataaata ttctatcgca
      721 tttgtcaatc agccgtgtat cgtgagtaac catgatcgtt gctttatttt tgtcttttga
      781 ttcttttgcc aagatatcga ctacttctcg tgccttttct gaatccaaac tagcagttgg
      841 ttcatctgct agaataatgg acggatcatt gtacaatgca cgagcgattg cgaccctttg
      901 ccgttctcca ccagatagtt cttctggata cttgttgatc aacttatcca cgccaagctg
      961 ctcaaataat tcgtttgctt ctttttgacg atgttcatgt ttaatcttat ctactaataa
     1021 taactgcttt tttactgtca gaaaaggaac cagattggaa gcttgcaaaa taaagccaat
     1081 ttctttaaaa cgtagtttaa cgcgttcgct ttctttttga ttactgaatt cttgatcatt
     1141 gataaataca gagccactgc taggcgtctg caatccgcct gccaaagtca aaaaagtact
     1201 tttacctgaa ccagaaggac cgatgattgc aacaaattcc cctttatcga tactcatggt
     1261 tgtttctttc aatgctttaa ctacttggtc gccatcttgg aaactttttt ctacttcttt
     1321 aaattctatt gctttcatgt tcttcctcct tcttatccaa ttgccttcaa cggatcgatt
     1381 tttacgattg tccgaacaga aaatagtgcg cctaacacag ctactactaa catcaacaag
     1441 ctaatgacgc caaaaaacag ccaattgctt tggaatggaa ccgcatcagg tagaacaagg
     1501 gatgttccca ctgttcctaa tagaccaaga agaataccaa caaatgataa aatgaatgtt
     1561 tgtgccacta cagaacgcgc aataaatcct cctgtgatcc cttgtgcttt cattacacca
     1621 aagatattaa tcttttgcat cgtcagtaca taaatgaaaa tcccgatcac aatagccgca
     1681 ataacgatca aaaatccgat cataaatcca aaagtcaata cttgcgcatt atatccaggt
     1741 aattcattga tgaatttaga aattttgatc tgttctaaat cttcaggtaa attactgatt
     1801 ttacctctga caactattgc gttaatccgt gcattttcac ttgtatcttc tttttcgaag
     1861 cggatttctt ggtaagcatt gatggttgta tagagaactg gtgagacatt gaatttggca
     1921 tgatccgtaa agccagttag tttaaacgtc ttgtcactac cagataactt aactgtatct
     1981 cctatggcta gaccatattc ttctttcaaa ctaatatcgc ctactgcctc atcatcgtta
     2041 tcaaaagctt ttccttcaac aagattgggc atgatgaatt gatttttatc gataccgaag
     2101 aagctaacat tgatttttcc ttcttctgta ctgtctttac tagtcacgac tccaggtgtt
     2161 tgggcaagat acgccacttt cccaccttcg acttcttctg ctatcttctt agtaatcatg
     2221 gacatcccta gatttgaatt tgcatctttc gaaagcacaa tcgaatctgc ctcccacttg
     2281 tcgactgctg ttcgattatc ttgagctaag ccataagcta acccagtcaa aaaaaatacc
     2341 aggtaggcga tcaaaaacat cacccctgca actaacgcat aacgtaactt tgaatgcata
     2401 atttcattta atgctaaaaa catttaactc ctcctcctga aataatcatt ttatacaaca
     2461 attttatcac ttgataaatt tttggcgaat attatgctca aaaaattctt ccgctatttc
     2521 attatatgaa ttttttattc tcgattgatt aatatttatt atatccccac acaataaaag
     2581 accgtgatat ttctcacagt cttttatcta ctctattgct atttgctctc ttagctagta
     2641 ctttaaaagc taactcattg aggtagtgaa aacgagaggt gtattcagac agaaatccaa
     2701 aatcctcaac tgctagctgt ggaatcgtag ttttgtgttt cacgatatcc gcaagcaggt
     2761 tcaaatctga taataagaat tcttggctta tcagctgtcc gccaattatt tctaatgtat
     2821 ccggatggta gatcagcttt gcatttactg ttttgttttc ttcgtcgtat tgtggaaata
     2881 gatcaaattc tcctgaacac gaaccggtat tttgttccaa gaatggcgct tcatcttctg
     2941 tcagccctac agttcctaaa aagtagtcta taatgactgc tgctgttatc ttttgtctga
     3001 ggtcgtaagc ggttggctga ccactcaaag ttagcgctac tgctcttgcc atatgtacag
     3061 ctcgagtaac taaaggtaaa aagagatgcc gtttattttt gtcaaaactg actgggacga
     3121 tatctccaat cgcatagatg tccggatcac ttgtctgcag atattcattg actatcacag
     3181 tctcatcctc atacaaagcc actttttctt ttaacaatgt cgtattagga cgagtttggg
     3241 cagaaaaaat gaccatatct gcctttattg tttgagacag aagttgtacg gatactactt
     3301 tctcttctga ttttttccaa tcgatcaaga attcatttgg ataaaaattt atttgcttag
     3361 tttgcagctg attttgaaaa ctgttgatca tatccttatc gaaatatctt gataaaacat
     3421 tcggcatata atcaactaga tgaataatgt atcctttatt tctcaaagca tctgctaatt
     3481 ccaatccaat gtaaccagca ccgaagatca ctacttcttt tgcttctttt aaagcggcaa
     3541 gtacttgctt agattgcact agatttttat aggtgtagac attttcagct tcaatttgtt
     3601 tatacaaggt gacaggagag gaacctgtcg ctacgattaa tttatcatag gttatggtct
     3661 cgttttttcc ttctttggat gcgaggatga gctgatgttt gtctggatca agttcagtta
     3721 ctctccattc ggtgatcagt tttgccccac tttcttgaag agtccgcatg ttatgggcaa
     3781 cttcagctaa actacgtatc tttcctttta aataaaggtt gattccattt gaaacatagc
     3841 ctagtccatc tttatgatct tcatcgatca gtaaaacttc tgctttcggt tgaagttttc
     3901 ttagatttag ggcagctgtg atgccttcat gggaagcacc aatgatcacg attttcatgt
     3961 tttattcatc cttaaattca atttttctaa ataacattct tttaaaaatt ggttcaaata
     4021 ggggaaatca taattatatt cgttgctagt gaatacaaaa actttagcgg acttctgtga
     4081 cgggtactct ttcaattggc tagtcaaaat gatatcatac tgcttttctt tctcgtattc
     4141 gactaaatca tatttgatcg tattcagcaa ttgttcctgg atgatttctt tcacgatatc
     4201 cgccattagc ctttcataag gaagatcata agcaatttgc agacgtaatt ctaactgctt
     4261 gatcagcgtc cgtaaaatag atgcatagcg agcaaataat tcaaagcggg cattttcctc
     4321 gtctacttca gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt cacataaaac
     4381 caggaattgt ttatcttcta cttcagttaa ccgctgtttt aatccttctt ggccaaaaat
     4441 attcatccat cctctaaaat aggcaaaatg agcgtgattt tgcatcactg tcaacgcaat
     4501 cttttgcgaa atctcttctg gaaaaacata aaaagagaaa tattcttcta tcgattgttt
     4561 aaacgttcga ttcaattgag ctactacgtc gtctttgtat ttttcgctta tcaaaaactc
     4621 ctggacatgc gagttatgtg tatctaatgt aaagttactg atcatgaaaa gataaaaaac
     4681 caacgtttca ttttgattcc aaggaaaggc atattgaaag aagtaacggg tcaaaaatgt
     4741 atggaggtcc aataaaaatt gtgtcgtttg aaacaatcca acaggaagat tgctataatc
     4801 tggtttggat tttttcgtac agcgcattcg cttcttcata atggaaagcc atatttcaaa
     4861 tttcaatcgt ccttcttctg taatagcaaa actaaactct ttttgcagca gatctaaaaa
     4921 agtgtttcct gataaatcac tgtattcttt tttgttttca agatagggac gtccaaacca
     4981 aaataattgg aaataaaaat aacgaatttg actttctgga ccggtcagcc gcccgctttt
     5041 tattttcaga tgaaactcat ttaaagaatc atttaattcc ttcactctgc gagacaaagt
     5101 agccgaactg atattaagtt ccatgacaag tttctgccga tcaaatgagc cattttccaa
     5161 cagataaaga agtatctgat attttgtaga atagcgcaag taacaatgat aataaacatc
     5221 gactgctgct atatttccat gtctgattaa taaaatattc ccttcttgga taacaatttc
     5281 caattgtttg atattttcca agttagcttg taaataaaac agatcttctt tcaaggtagc
     5341 ccgatcaact gataagcggg taagtacgtt attaacggaa acctctcctt tttcctctaa
     5401 taaaagttgg aaaagtttga tttgccgttg ttcatttttt tcaagcaaac tgaaaagttc
     5461 caagtccact ccttcaagca tttctgattt taacagcaga agtttccttc tcatccttga
     5521 aaaagaggat ctgtgacagt tatcacagat ccatacaaat tctaatcact tagctggttg
     5581 gcccagctgt cagttcccat gtgaccatgc cttgatacgt tgcgccaggt tgagcattcg
     5641 tactgctatt tacctctaag agtaaaccat cggtatcggg aaagtcaacg agaacctctc
     5701 ctttagctgt tccatcactc tcataaatca acgtgttttg accttgctga atcaattgct
     5761 ttgatccaga atgattgaag tataattttg ttccgcttaa ttcttgacct tgatcattct
     5821 taaataaaga agtcaatgta cctgtcagtt gccaaggaga cgtgcttgga agccgagtat
     5881 cccgcacatc aaaagaaaaa tcgccttttg attcataata acttgtcttc aacggtttgg
     5941 ggagatcgtt accaaatgta aaatcatctg gtacattaac taatgttaat tcttgtttgg
     6001 ggacaagaat tgttttagca gatgtttgga caactgggtt aggaagctta gcaccatctg
     6061 ctaatgtaat attgctggaa agtacagctg cctttgtcag tatgctgacg gattgtaaag
     6121 aagtatctac agtatttaat tgatagttgt aagtaagatg aatggtatct cctattttgc
     6181 tcaatagatc tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg atagttgtac
     6241 catttaattt taaggaacca ttgatataag tagtattttc aggaagtgag atcgtgattt
     6301 ttttgtcggt tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt tcttgaatcg
     6361 tatctcctga tagagcagac aggggatctc ctgtctcagg ttgtctgcta aggtttttta
     6421 gctgactatc tgcactcaca gatggaaaat tagccactgt tatttgagga agagtggtga
     6481 ttccagcttg ttcagactgc catttgtacg caaaagacgg atacatgtta gtaagtacat
     6541 cgtccaccaa gcgataatca aaataatatt gccctgaagc caatgcaggt agtgtagaag
     6601 tagctgtatt tacacccccg ttatttgatt ggatcgtttg gaaaagttga tctgtccatc
     6661 ctgtcgctaa tttttgggtt cctttcgaat aaagcctgta atggatcgaa ccctttttta
     6721 gttcagttgc tgaccaattg gccgtgactt ggtacgatgc agaagaaagg agctttcccc
     6781 actgactatc ggctgttaag tttgttggta aaaccgttcc gtctagcttg tactgctcca
     6841 atacctttaa agcaacagga gaaagcaatc cgccttgcgc atctttccca ctgtacacga
     6901 tattggctgt ctgattcaat ccacttgtgc ttgctggagt atgagtcagt attgctcctt
     6961 gtgtaacgtt ttttcctgta aaatagttga ctacgcgatc aaagcgacta aatggcaact
     7021 gatcaaaacg tccttcagtc agccccatcg tcaatgtccg actattggta gagcctggaa
     7081 ggttctggtt atttccatcc acgtattctg tggtatcagc aaatgtatta tattttcctg
     7141 ttgaatcact tggtgagatg taactattcc acgtatttat ctctgcactt gcagaaaatg
     7201 catatgctcc tggattcaag tttccgccag ttaatttagg ataaaatcta cctgcagctg
     7261 gagctaaaat cgaattgaac atacgatctt ggataatatt attcgaatca taaaaatcaa
     7321 cagaaaaaac ggccttttta gacatagaag gttgtaattt acccttggtc aaaattttgt
     7381 ttggactcgt atcatattta tctccgtttt ctgtcatctt cgcgacatta gctgtaacat
     7441 caaattgaag cgggatatcg tccacgttga attcgcctcg cgtaataata gcaataatat
     7501 ctgtcaatag agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg
     7561 ctgataataa acttccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt
     7621 tgttccagtc aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga
     7681 tcgccacaaa tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt
     7741 tcacatggct ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat
     7801 cgttcaaata agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct
     7861 tcccgccatt gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg
     7921 gatcataaga aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa
     7981 atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt
     8041 gtagcaacat gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc
     8101 cataattcta taccaatgat caaataaaga aaacggatct tttgttcatt cgtctccggg
     8161 aatacgccat gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg
     8221 ccattgactg aaccacttgg atgcggaatt ggacttagat cataaaatgt aatcgtacct
     8281 gaacttttgg tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata
     8341 atccctccca ataagatcga tgttttcaac gaaatcacct catcatgttg tttctccttt
     8401 tttctttttc ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc
     8461 aaccaatcct gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc
     8521 atttactttt tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt
     8581 actgtcgcct gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc
     8641 agctaaatca agattcaagt gaaaattcga atttggtgca actaaaaatg cattagacgt
     8701 attttcaata taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat
     8761 aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc
     8821 caaatctcct gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac
     8881 gactgcgaga ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt
     8941 ttcagcttct tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt
     9001 tgctgggatt ttcacttcta taggtacagt tgtttctgca tgtgctgata cattgatttc
     9061 ctttttcgca atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg
     9121 gtttttttga ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac
     9181 ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg
     9241 ttccggtgcc actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa
     9301 taccggtttg attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat
     9361 gaaaaataga cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt
     9421 ccaccacatc ctttcaacaa caactgcggt aaaaaatcct tcacctatag tattttcacc
     9481 gcagtttgtt gaatttattt catactctat gctactgggc cagccacgag gttccaagtc
     9541 aaatcagccg tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga
     9601 tcataaacag aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc
     9661 gctgcacttg ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta
     9721 tctgacgtac ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt
     9781 gaaccgttta caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct
     9841 gcacctacac ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct
     9901 acttgatacg tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcgggaact
     9961 acatccagtg ttaaaggacc tgtctggcca gttgctggat tttccggatc tgctggctct
    10021 actggttttg aaggatcagt agggttaaga ggctccacgg tatttgttgg ttcagtaaag
    10081 gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct
    10141 agaagaagtg ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc
    10201 tttttcttag tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc
    10261 cgcttacttg aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta
    10321 catgaatatt cttccttttt tattaa
//
LOCUS       DAFGFZ010000099        10285 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000099 DAFGFZ010000000
VERSION     DAFGFZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.101"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            397..1620
                     /locus_tag="LR726_002250"
     CDS             397..1620
                     /locus_tag="LR726_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBL6217295.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGRTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGTEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1655..2422
                     /locus_tag="LR726_002251"
     CDS             1655..2422
                     /locus_tag="LR726_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL6217296.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2614..3012
                     /locus_tag="LR726_002252"
     CDS             2614..3012
                     /locus_tag="LR726_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL6217297.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3318..3692)
                     /locus_tag="LR726_002253"
     CDS             complement(3318..3692)
                     /locus_tag="LR726_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217298.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3714..4091)
                     /locus_tag="LR726_002254"
     CDS             complement(3714..4091)
                     /locus_tag="LR726_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217299.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(4081..4476)
                     /locus_tag="LR726_002255"
     CDS             complement(4081..4476)
                     /locus_tag="LR726_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL6217300.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4543..4728)
                     /locus_tag="LR726_002256"
     CDS             complement(4543..4728)
                     /locus_tag="LR726_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBL6217301.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4810..5064)
                     /locus_tag="LR726_002257"
     CDS             complement(4810..5064)
                     /locus_tag="LR726_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217302.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISTKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(5086..5331)
                     /locus_tag="LR726_002258"
     CDS             complement(5086..5331)
                     /locus_tag="LR726_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217303.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(5338..5622)
                     /locus_tag="LR726_002259"
     CDS             complement(5338..5622)
                     /locus_tag="LR726_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217304.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(6113..6652)
                     /locus_tag="LR726_002260"
     CDS             complement(6113..6652)
                     /locus_tag="LR726_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217305.1"
                     /translation="MYNKSVNKLINLQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(6765..7031)
                     /locus_tag="LR726_002261"
     CDS             complement(6765..7031)
                     /locus_tag="LR726_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL6217306.1"
                     /translation="MSYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(7033..7254)
                     /locus_tag="LR726_002262"
     CDS             complement(7033..7254)
                     /locus_tag="LR726_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBL6217307.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            7474..8292
                     /locus_tag="LR726_002263"
     CDS             7474..8292
                     /locus_tag="LR726_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6217308.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            8296..8592
                     /locus_tag="LR726_002264"
     CDS             8296..8592
                     /locus_tag="LR726_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217309.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETRSSLVDK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(8773..9117)
                     /locus_tag="LR726_002265"
     CDS             complement(8773..9117)
                     /locus_tag="LR726_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217310.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDSKVAEVKNKPTDHNPIQQEHPSTVTSGHSAKKEKPML
                     KGFNAPSNKDLL"
     gene            complement(9137..10192)
                     /locus_tag="LR726_002266"
     CDS             complement(9137..10192)
                     /locus_tag="LR726_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBL6217311.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
BASE COUNT         3380 a         1635 c         1758 g         3512 t
ORIGIN      
        1 tatttataat atatatttta atctttaaaa ctttagcaac ctccaaaaag gctttatatc
       61 aatcttaaat agattttagg gtggacataa agcgatgtga atgtggacaa aaagcgatgt
      121 ggtattccga atatatccaa aaaaagaaaa agtatgtgaa tttatttcta aaaactagaa
      181 aaatatttgt tttttaattt tggtaatttt tataatttcg tatttttttg aaaaggattt
      241 ttattttgaa aatgttctaa aatagcgatt tctgctgtat tttcaatgaa atttgattgg
      301 aaaaaataaa atgtgtttta ctgacattga gttatgttat aaatttacga tacatttctt
      361 attgtaacgt atcgaaaaca ggtctatact tattcaatgg aatcgtacca gttccaagtt
      421 tttaagctcg taccaagctt agaaagaaga ggcaatcgta tgaaaataac agaaaagaat
      481 gtaaacccga aatttgaaac taccgaaagc aatctaaaaa agtttgattt aactgttact
      541 ttatcgaatc aacttgtaga tagcattgtt gaaatggatg ctatgccaca aaaaatttat
      601 ctttacgcta taggacatct tgaccctaaa aatccgcctt ttaataatac cgtcaaagta
      661 tctaaaaagg ctttatttgc gttgatgtct tctagttcat cttcttcaaa acattcaaga
      721 ttaaaacaag ttattgaaaa attgcatcag caatctgtca ttaaaattca aagaactaaa
      781 cctaacggta agactgaata caaagtcatc tcaccgctag aaattactga attcaatgat
      841 ttttctgatg cagtatctat taaatttagt agttcaatta tgcctttatt aacgaactta
      901 gaggatcatt ttacgcaata tacatttgat caagtagtta acttgacaag caagtatgca
      961 atcagttttt ttcaattatt cactaaagat tttaatctgt atgaagcaaa tagagataac
     1021 ttaacatgga gtacaaaatc acttgataga ttaaaaaatc ctgttttctc cattgcagat
     1081 ttacgaaaga aattcagtat tgagaaaaaa tataaagctt ttcgagactt tgaaagacgt
     1141 gtaattaaag aaccgattgc ccaactgaat aaatacactg attttaaaat tgactatgag
     1201 cttatcaaga agggtcgtac ttatacaaat ataaagttct tgcttacacg taccatgtca
     1261 gttccggtac cttataagca agaacaaaga gaccctgctt atttaaaagg cacagaagat
     1321 aaaacagaga aaatgaatca atcgtacttg aaagctatga atagcaaata cactaaatta
     1381 ctaatcaaaa actttttgat cagttctgcc gacttatgcg atcttaaact tatgagccga
     1441 cttcagaata aagtttatcc gctttatgat cgtttatcca aagatagagg atttgaatca
     1501 gttgtcaatc acctatctta tgttgctact cataaggagg attacacaat aaaaaatact
     1561 gttaaatatc tcgaaaaatc aattacgagt tatttgcctt atgtgaacct ttatgattaa
     1621 caactgaaaa aatgcgtgaa ggaaggaaaa acttttgaaa aaagatgaac tgacaaagta
     1681 caatgaggag ttcttagcaa caattgacat gaaaatggat ttaaagtcgt atgaacttta
     1741 tcaattgctg ttcagttata tctcacccac aaagctaaag aaaacaggcg tagtagattt
     1801 acctgttaaa gcgattatta attacatgga tgttgatcaa aatcttgaaa cggaactaaa
     1861 aaccatagtt aacactttgc aaagacaagc agtttatgaa atttcgaatc tagacaaaaa
     1921 atataagacg tatactactg tatctgctat tgatcggatt cattgggatt tagaagagca
     1981 tagtgtatct ttcagttttt cagaagatgt aattccctta attgtctcaa tcaaaaagaa
     2041 gtatccgttc tttgtttttg aagacttagc atcttttcaa aaaaaatata gcatcgtgtt
     2101 atacaaatat ttggcgatgt attataaaaa atatcttatc gccaaaaata aaggagattt
     2161 tgagaaagct gaaatatata agaatcccct gattgaaata gttaaaattc gtgagattac
     2221 gaaaacagaa aatgagtata aaacaatatc gaattttgat agacgtgttt taagggaatc
     2281 agttgaggaa attaatgaaa aatcaatgct gaatattgat tttgctaaag ttagaaaagg
     2341 cagaaattac aattttgctg tccagtttaa tatttctagt gagtttgatg aaaaaactta
     2401 tagagaaaaa aaattaggtt aatgcaaaat atatcttact cgtttgaaga tgcttcagac
     2461 gtatgctaag atatatagtg ccttgggagc aaggtattaa tctcctttag aggttaatta
     2521 gcttgtgatc gcctatcaat ttttttggta ggcgattctt ttttttgaaa caaaaaagca
     2581 aaaattattt tttaattaga gaggtgcaat gcgatgaaat actttttttg tgcagtattc
     2641 gaatggaaag gaaatgaata tgcagttaaa ttcccagatt ttccagactt atatacacat
     2701 ggtgaaactt tagaagatgc ttttgagatg gcgcaagaag tattggaagg atacttggtg
     2761 cttttagagt tgcaagaaaa agaaattcca tcgcctacgc ctgttcaaga gttaaatgca
     2821 agaatcaagc gatctgagaa tgttaattta tttgtcgtaa aagctaatac gaaagttgct
     2881 aaagctcgaa acaagcaaaa ttatgatcga aaatcagtga ctgtggaaca ctatttaaat
     2941 gttttagcgg ctgaagagaa tattaatctg tcgaaagctt tgaatgattt gttgagagaa
     3001 aagttgattt gatgtctctc agatgatcat attaagcaaa aacagcttgt tttaggacgt
     3061 atggatttaa gaaagacaaa ttatcaaaga atatattaaa agctctcaga agccttctga
     3121 gagcttttaa attgctatgt attgtactgc atctgctcaa attgattttc ttttcatgct
     3181 taccatgtat ctagttttcc atcaagtaat ttttagatat gtattaagta ataaatagca
     3241 aatttgcttg atataagctc gaattaggtg ctttataatg tgttaggata aattatacaa
     3301 gaaaatgtca aaagctctca gaacgcttct gagagctttt tatcggtagg cttgtttaac
     3361 gtgatcttga aagataccct tttgatgttt ctctcgatcc ctgcatcgct aattactaag
     3421 tgttcttcaa gtgtgatgta ggtatataaa ttaagcatca aaatggtgta gataagcaaa
     3481 aaactcccta aaactaagtg ttgattataa aaatctgact tataaaaaag aactgagaat
     3541 gttagtgttg attcccaaaa aagaaacaaa attttgtatt tccaaagata gtggatcaag
     3601 cgctgtgctt tacccatctc aaataattta taataacctg ctaattgatc agcaagcgta
     3661 tcgttaacgg tttctgtttg tgtttgtttc atctttctct tctcctgttt caattaatct
     3721 aaaatttcag attgagcaag caactcattg tggtagtcgg tttgaaattc aaataacaat
     3781 tttgccttct taacgaggtt taaagcgttc gttagtgatt cacttaatgc ttcaacatct
     3841 ttaactgatt ttgcatgttt cattttttca gtgtgcgtat agcagaattc aggtattgag
     3901 ttaggggttg cctgttcaat aaacatctca attcgcaagt tattatcctc aaaaataatt
     3961 agatcattgg caaagtgttt ggtactaacg ctaatgccag ttgtatcttt taatagatta
     4021 ttgattaacg ttcgagagaa atagtttaag tgtgtagatt tcataggttc ggtctccttt
     4081 ttacttttca agcaactctt cttctagtgc tttttgaagt agttgagaga agtttatgtt
     4141 tttctccttc cctaaaagaa ctaaccattc cggaacacta acattttttc taattgtttt
     4201 tgtatttaag cttcttcgga aaagtttaat gtttagttgg atgattacta ccacttggtt
     4261 ggctttcaaa gttaattctt ccactgctga tggtttagga aattctgcat ctggtacact
     4321 atatagcgct aatcctaaag catccttagc gtttaaatat gcttcttcaa gactatttcc
     4381 aaaggtagat atttccggta agtcagggaa aacgatgtta taagcttctc cttctttctc
     4441 gaagattgcc ggataaaaaa tattttttgt cttcatttcc tcgctccttt tctagtgtaa
     4501 gagaggatag tatgcctaat accaaatagg aaggcaaggc tgttaaagcc ctgcttgctt
     4561 ccagatatta gcttctgttc ctagtggtac atcaccattg tggatcggaa caacgaccgt
     4621 tcttttagtc ttggaattat aaaattttgc atgactgcca gtttgtccag tctttttgaa
     4681 gccgttagat ttgagaattt ttacaatttc tcttgctgtc ttaggcatag ctaccctccc
     4741 tttacaaaat ctattataca cattgttgtg tatgtaatca atagaaaaag aggatgtttt
     4801 attgcaaaac taataaaatg atttattggt tttttcagca aattgaatgt tattagcgtt
     4861 gcaaatattc ataatttccc aagtatcatt agaagtatag gtatctaaga attcaatgac
     4921 agttacggtt gattcgtatg gttcataagc caaagccttc tccaagtctt ttgttgaaat
     4981 gatcaagact ttttcaattt cctccccttc ttcatttgtg aaaacgtatt ccaaagtgat
     5041 ttgttcttca tcggtataat tcatattgtg ttctcctttc ttgtattaag aaaaatggat
     5101 atttccaatt gcatcccact cggcaccatt gtggtataag acgtgaccga gcgttacgcc
     5161 tttgaaatgc tcttctaaat acatccaaac gcattcaagg ctagtacctg ctttgagatt
     5221 gtagaagtct gcatccaata ccttttcaga agttatcgga tcaatgcgag ttggtacctc
     5281 aataaattca gtccaaattt ctgcaaaagt cttaccttcc aaagtccaca ctccctatca
     5341 attaattcca aaagcaaata gagaaatcaa gcgaccgtca atagtcactt caacaactga
     5401 aaatgccgtt gaaacagttt taacatccac actttccaat ttcccgatga aggaattttt
     5461 gtgctttaga atagctttct ttaactgtct gttatacgct tgacgactgt taaacttttt
     5521 gaattctcga tccttgatca gctgtttaac gatccctgca ttaattgtat agctttctgc
     5581 aatagcaacg tgagtaggtt gctgtaatgt agctgttagc atattgtcac tccttcctaa
     5641 aagttaatta gcttgtttac aattacatta tacacactag tgtgtataat gtaaagagag
     5701 gttttttgct gatggatcag ctgtgctaaa caaaagaaaa cgatagagta acaaaaaaag
     5761 tcagcaaagt taaaaaggat gaaacggcta gtccggttgc tttgcttgtg taagtttcgg
     5821 ctttcttata gatcaaatag gaaattataa gtgatattcc agtaaagaat aatgtactag
     5881 ctgtcaccaa aagaaatagg ttaatcatac gatacactcc ttctataata ttggtatcag
     5941 atacgtttaa atgcgtttct gagctgtttt ggatcatatg gtgatgtttt agctctaccc
     6001 taaaatcaaa gcccagaaaa gcatttaaaa gcgttcgtac ctaatacgtt tatattcata
     6061 tacatataca taaacataaa cgtatacacg tttatgttta tgtatttaag ttttaggcta
     6121 cttccttaac atctcctgct gatagtcgag tgaaccgagt gagtgatcca tcttgtagtc
     6181 gaaaaataaa gctacgtttg agtttatact gataaacagc gacatacgat ccatcccaga
     6241 ttgagtaagt tttacctgca actaataagt catcaagttt tggtttattg ctttcttctt
     6301 taattctatc tagttgtcgc ttaaccattt tttcttgatc accgacaaag aatttcaacc
     6361 attttttcgg aattttctta aagtaagcag gactgtcatt taagaagtag attggtttgt
     6421 acatttcatc tcgaaagatg aagctgtcaa tatcgtaggt tccttctgca tctttgaaaa
     6481 ggataattcc ataggtcttt gttactttgg tatctacgat ttccaaacca tttttggcgt
     6541 accctttctt ttcttcgatc acttcatttg ctgatgaata agcaccatac catcccataa
     6601 aaaatcgctc cttttgtaag ttaattagct tgtttacact tttattatac acaccaatgt
     6661 gtataataac aagaggatat tgttcttttt taggtaaagt gggatcgtaa acagtgattt
     6721 tgttgggtta acaaaaaaac agacaagcct tatcagcttg tctgttactt ataaacttca
     6781 cttctgtgac caatagacaa tataagaatt gtcacttcat gctcgctaat atcagctatt
     6841 atacggtatg cacctaccct atatcgccag tagcctgatt tattagctgt tagaccttta
     6901 ccatgctttc ttggatcatc agtgcctaca agatttttct cgatccagtt taggataacc
     6961 tttgcctgaa acttatccat tttttttagc gctttttgag cttcacgttc atatcttact
     7021 tggtaactca tcttataagc ctaactcttt tagcatatct tcgtgggaaa tactttgatc
     7081 gttttcctca tgctctttca ttagttcctt gtaggtggca aaatctacta acgtttctgc
     7141 tgtttgcagg gaattactac gcataaattc agaaatgtta tctccgttga attcagcaac
     7201 tttttcaaga aaatcttttt cttctttaga tacacgaata cttattactg gcatagttta
     7261 tcaactcctt ttcaattaca ttgtactacg attgtatgac aaatgcaata gttatatata
     7321 tgaacataca cataaacata tacatataca tgtttatgtt gtagaatata aaagcaaata
     7381 taaatataaa cacgtttatg tatataaaca tatacataaa cgtgtttata gtgtataatg
     7441 gagttacaaa aggttaataa gggaggttta atcatggaaa agaagttgtc aatcattact
     7501 ggaaacattg aaaaaggtgg aacaggtaaa acaacaacaa tctacaatct aggtggatat
     7561 gctgtcgaaa atttaggtat gaaggtgctg tatattgatg aagataagtc gcaaaaccta
     7621 tcaaaacgct ttcctgatgc tttcagtaaa gcgaaaagag agaatacaat caatcattta
     7681 tatcttcatg gatcagctga acctttagct ataaatgaga atatggatat tttagtagca
     7741 ggtgaagatt taaaacaagt tgagatgcaa atgcgtgata tgcctaacaa cagaactcta
     7801 ctgtttagat ggatcgttgt taactatgaa cagcttaaag agaagtatga tctaattctg
     7861 attgatactc ataatgatgc aggtctacta gctcaaaatg cttgggctgt atctgatctt
     7921 gtcgtaggca tttctgatcc atctaaagat ggttttgaag ctttgctgaa gcttggtaaa
     7981 gatattgatg cactagcaaa acagttgatt gatattaaga ctggtgaaac actgatggaa
     8041 actaaatacg ctacgatcgg tagcaggatc aaacataata ctaattcaag tcgtgagttc
     8101 ctagaattgg taccaacgtt atctaattat cttggatatg tacaagagaa ggaattgctt
     8161 aatgcaacta gcttagaagg taagaatatt gctgagttcg ctaaggataa gaatatctat
     8221 cgtaagaata aggatttctt tgaaagcacg tttacgctat acaacaaaat aattgatacg
     8281 gcacaagaat aaggagtgat tgaagtgaaa gaaaatagat ttaaaaaatt cgctgaagct
     8341 gaagtagaag aagttcctga agtggttgaa gcagttgaag aacctaaaca aacacctaaa
     8401 gaaacaagaa gttctttggt agataaacta aaaagtgata gttctgaaaa gaagaaaaat
     8461 tatcaattca cgcttcaacc atcgcaaaga aagaaactat ctaaaatcag cgaaaagtta
     8521 ggaattcgat cagattcagc tttacttggc gaaatgatag ataacttgta tgatgaagtt
     8581 tttggtgagt gatcagttta aagctgatca tttttttatt ggaataagcg gttaggtata
     8641 gcttaactaa atttcatata cgtgtttatg gttatatacg tatacatgtt tatatacatg
     8701 tatacgtata tgtttatgtt tatatttgta cataataaaa aggctaggta ttgtaacctc
     8761 gcctttttat ttttaaagta agtctttgtt acttggtgca ttgaaccctt ttagcattgg
     8821 cttttctttc tttgctgaat gaccggatgt aaccgttgaa ggatgctctt gttgaatagg
     8881 gttatgatca gttggtttat tctttacttc tgctactttg ctatcgtttc ctgcttcaat
     8941 aattgtaact tcattgttct ttacacgccg atataggttc aaagcttttt tgatcgtctg
     9001 agagtattta acgcctagtg tttcatattc ggattcaaga aactctgcta aaggtaactc
     9061 gctaaccttt tctctattga ttataactac ataacgctct gattttccct tattcatgtt
     9121 gatctccttc gctgttttat tgagttaaac caaacttgta aaagcctttt acattagaaa
     9181 attcactgtc ttcaacaaac aaggtatgcg gaattttatc ttttacaagt tgtttgtcga
     9241 taatagaagc tccaccacca gtaaagacaa ctttatctaa ggttgtgaag tttttgaatg
     9301 tagctaaaac ttctgcaaca accatattgg tgtatgtttt tatttctctt tcaactactt
     9361 ctgaaatatc aaaactttca tttccgcttg gcttaaatag atatacgcct ttttctttgc
     9421 cttctcggat aatcttttca agttggaaag cattaggact gatcggaagg tcaagcatat
     9481 ttacgatacg attatataaa gtaaaaatgc cggtatcctt ttgttcacga ttgttttcgt
     9541 caatttcaaa attttggaca acatcaaaaa gtttcgtgcc accgccaata tcaacaaaac
     9601 cgatcttctg actgatcaat gtttcatctt gtacactacc atcatcagca agtaggaggt
     9661 tatatagtgt tccaatcggc tgtggcagta cccaaacggt atctacgttt atattataag
     9721 ccttaccgtc aacttcaact gaatgttgtc gtgtggcttg ttgcatcacg tatttgacga
     9781 ttgtttcggt atagtcggat gaaggcagac ctaaaactag attaacggtt aaaacgtcat
     9841 cagattgatc ttctaaacgt gctaggttag ctaaagagaa agataggata ttttggtagg
     9901 tttcctgctt gtatctgctg atcccaaaac ctagtgattc tatcacgtag tcatcttttc
     9961 ctagctcgtg tatatccttc ccaaagtaat agctttcagt agtgtttact aggctatatt
    10021 cagaaacgtt tagtgatcgt tttaatgagg aacctatatt agaaaaacgt cctgaattaa
    10081 tgatattttt gttcaaaata cgagagggaa gtgagagtgg agaaacgtgt gtactcattt
    10141 cttttactga tttattccct aagtcaacgg aaaacaataa tggttctttc atggtatgac
    10201 ctctttctgt ttgtgtttat ggtatttcgg tacgaaaata gtttacacct atttactatt
    10261 agttgcaact gtttataaga aaaaa
//
LOCUS       DAFGFZ010000100        10272 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000100 DAFGFZ010000000
VERSION     DAFGFZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.102"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(253..1101)
                     /locus_tag="LR726_002267"
     CDS             complement(253..1101)
                     /locus_tag="LR726_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL6217312.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1100..2167
                     /gene="gnd"
                     /locus_tag="LR726_002268"
     CDS             1100..2167
                     /gene="gnd"
                     /locus_tag="LR726_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL6217313.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2169..3170
                     /locus_tag="LR726_002269"
     CDS             2169..3170
                     /locus_tag="LR726_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL6217314.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3167..3451
                     /locus_tag="LR726_002270"
     CDS             3167..3451
                     /locus_tag="LR726_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL6217315.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3484..4281
                     /locus_tag="LR726_002271"
     CDS             3484..4281
                     /locus_tag="LR726_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL6217316.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4295..5128
                     /locus_tag="LR726_002272"
     CDS             4295..5128
                     /locus_tag="LR726_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217317.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5146..6003
                     /locus_tag="LR726_002273"
     CDS             5146..6003
                     /locus_tag="LR726_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL6217318.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6025..6534
                     /locus_tag="LR726_002274"
     CDS             6025..6534
                     /locus_tag="LR726_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217319.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6571..6972
                     /locus_tag="LR726_002275"
     CDS             6571..6972
                     /locus_tag="LR726_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217320.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7084..7437
                     /locus_tag="LR726_002276"
     CDS             7084..7437
                     /locus_tag="LR726_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217321.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7471..7914
                     /locus_tag="LR726_002277"
     CDS             7471..7914
                     /locus_tag="LR726_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL6217322.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7931..9016
                     /gene="uxuA"
                     /locus_tag="LR726_002278"
     CDS             7931..9016
                     /gene="uxuA"
                     /locus_tag="LR726_002278"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL6217323.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9009..9722
                     /locus_tag="LR726_002279"
     CDS             9009..9722
                     /locus_tag="LR726_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL6217324.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9929..10078)
                     /locus_tag="LR726_002280"
     CDS             complement(9929..10078)
                     /locus_tag="LR726_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217325.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3431 a         1628 c         2236 g         2977 t
ORIGIN      
        1 aaaataagaa acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta
       61 actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat
      121 gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca
      181 aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt
      241 tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg
      301 acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag
      361 ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat
      421 aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt
      481 ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt
      541 gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat
      601 atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc
      661 agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat
      721 aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt
      781 tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat
      841 attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa
      901 atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat
      961 gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa
     1021 aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg
     1081 tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc
     1141 cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt
     1201 tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc
     1261 tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt
     1321 aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc
     1381 aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca
     1441 gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa
     1501 actttttcca aaactcggac caggagacat tattattgaa ggcggaaact cttattataa
     1561 agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg
     1621 gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa
     1681 agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct
     1741 gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata
     1801 tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt
     1861 tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat
     1921 ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt
     1981 ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt
     2041 tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc
     2101 agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg
     2161 tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct
     2221 ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc
     2281 aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa
     2341 gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt
     2401 cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa
     2461 tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta
     2521 atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat
     2581 gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg
     2641 gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg
     2701 ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag
     2761 tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag
     2821 aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc
     2881 tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa
     2941 gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc
     3001 atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg
     3061 aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc
     3121 ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat
     3181 ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat
     3241 ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg
     3301 aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca
     3361 atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac
     3421 aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat
     3481 ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct
     3541 agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa
     3601 gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg
     3661 attgattccc ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat
     3721 gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca
     3781 caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa
     3841 gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct
     3901 gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct
     3961 atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa
     4021 gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag
     4081 ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt
     4141 ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg
     4201 catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa
     4261 ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag
     4321 tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac
     4381 gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga
     4441 agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc
     4501 tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc
     4561 attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct
     4621 cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga
     4681 caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac
     4741 ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc
     4801 attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt
     4861 agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa
     4921 agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga
     4981 tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc
     5041 gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga
     5101 cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg
     5161 gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg
     5221 agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta
     5281 ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca
     5341 gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt
     5401 actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag
     5461 atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca
     5521 ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta
     5581 ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg
     5641 tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct
     5701 acaggcctaa agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg
     5761 ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt
     5821 gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata
     5881 ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc
     5941 acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta
     6001 taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt
     6061 ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt
     6121 gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga
     6181 agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat
     6241 gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac
     6301 gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa
     6361 aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa
     6421 aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca
     6481 tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat
     6541 gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg
     6601 gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt
     6661 ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac
     6721 gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa
     6781 gttatctgta cttattacga tacacccgtt catgcattag gaagttattt catgtttgat
     6841 tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc
     6901 agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg
     6961 ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga
     7021 aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta
     7081 acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat
     7141 gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct
     7201 attctttttt atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat
     7261 ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat
     7321 tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta
     7381 cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta
     7441 aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt
     7501 aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt
     7561 gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa
     7621 aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca
     7681 agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc
     7741 acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg
     7801 cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta
     7861 actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa
     7921 ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt
     7981 aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat
     8041 gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca
     8101 aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac
     8161 agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg
     8221 tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt
     8281 gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca
     8341 gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc
     8401 gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat
     8461 gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg
     8521 cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg
     8581 ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc
     8641 tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt
     8701 agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga
     8761 gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga
     8821 tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga
     8881 tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc
     8941 aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg
     9001 agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa
     9061 ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc
     9121 gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg
     9181 gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata
     9241 aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt
     9301 tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa
     9361 aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag
     9421 gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa
     9481 tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag
     9541 caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc
     9601 ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact
     9661 atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct
     9721 aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag
     9781 atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag
     9841 aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga
     9901 gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt
     9961 gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata
    10021 gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag
    10081 ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc
    10141 atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag
    10201 ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact
    10261 ttttcttcga tt
//
LOCUS       DAFGFZ010000101        10158 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000101 DAFGFZ010000000
VERSION     DAFGFZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.103"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            40..1686
                     /gene="mdcA"
                     /locus_tag="LR726_002281"
     CDS             40..1686
                     /gene="mdcA"
                     /locus_tag="LR726_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL6217326.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            1788..2087
                     /locus_tag="LR726_002282"
     CDS             1788..2087
                     /locus_tag="LR726_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL6217327.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            2077..3753
                     /gene="mdcD"
                     /locus_tag="LR726_002283"
     CDS             2077..3753
                     /gene="mdcD"
                     /locus_tag="LR726_002283"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL6217328.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            3757..4350
                     /locus_tag="LR726_002284"
     CDS             3757..4350
                     /locus_tag="LR726_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL6217329.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            4374..5450
                     /locus_tag="LR726_002285"
     CDS             4374..5450
                     /locus_tag="LR726_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL6217330.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            5475..6344
                     /gene="mdcB"
                     /locus_tag="LR726_002286"
     CDS             5475..6344
                     /gene="mdcB"
                     /locus_tag="LR726_002286"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL6217331.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            6455..7219
                     /locus_tag="LR726_002287"
     CDS             6455..7219
                     /locus_tag="LR726_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL6217332.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            7708..9801
                     /locus_tag="LR726_002288"
     CDS             7708..9801
                     /locus_tag="LR726_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL6217333.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            9826..10128
                     /locus_tag="LR726_002289"
     CDS             9826..10128
                     /locus_tag="LR726_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217334.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         3391 a         1793 c         2161 g         2813 t
ORIGIN      
        1 gacgccacgt ttctatatca aaaatggaga tgactaatta tggaaaaacc aaaccgcaac
       61 tgggcttcta aacgcgaaaa aaaagcgcag cgattggaaa aaatcagcaa cttgctcaat
      121 ggcaagtatg tagaaactga aaaaatcgta gaagtaatgg aaaaactgat tgctcccggc
      181 gacaaagttg tattagaagg cgacaatcaa aaacaagcaa gttttctttc agaaagtctt
      241 gaaaaagtag atgcagaaaa agtgaatgat ctgcatttga tcatgtctag tatctcacga
      301 ccagaacatt tgaatatttt tgaaaaagga atcgcaagca agatcgattt ctcttatgct
      361 ggtgcacaaa gcttgcgtgt cgcccaaatg attgaagacg gcacaatgaa attaggtgat
      421 atccatactt atttggaact ttatggacgc ttattcatcg atttgattcc aaatgttgtt
      481 ttagtagctg cagataaagc agataaagac ggcaacttgt acacaggttt caatacggaa
      541 gaaacaccaa caatcatcga agctgcagct ttcaaagatg gtatcgtgat cgttcaagtt
      601 aacgaagttg tcgattcttt accacgtgta gatatccctg gtgactgggt agacgttgtc
      661 gtgaaagcag ataaacctta tcaattagaa gctttattca cacgagatcc tcaaaacatc
      721 acagaattgc aaattctaat ggcaatgatg gcaatcaaag gaatctacgc agaacacggc
      781 gtacaatctt tgaatcacgg tattggattc aatacagcag ctatcgaatt gttactgcca
      841 acttatggtg aatcactagg gttaaaaggg aaaatcgcga agaactgggt attgaaccca
      901 catccaacga tgattccagc catcgaatca ggttgggttg aaagcatcca tagttttggt
      961 ggcgaagttg gtatggaaaa atatattgct gctcgcccag atgtcttttt tgtcggtaaa
     1021 gacggcacga tgcgcagtaa ccgaacatta tcacaagctg caggacaata tgccatcgat
     1081 atgtttatcg gttcaacctt gcaattagat tacgaaggaa actcttctac cgtaacaaaa
     1141 ggacgtttat ctggttttgg cggtgcgcct aacatgggac ataatcctgg cggtcgtcgt
     1201 cattctacac cagcatggca atcacttcgc gacaatgatg atccattagg gaaaggccaa
     1261 aaattagttg ttcaaatggt tgaaacatat ggttcaaata aaaaaccagt ctttgtcgaa
     1321 gaattagatg cactagctgt tcaaaaacaa gcaggcttag ccaatgcacc agtgatgatt
     1381 tatagcgaag atactacaca tatcgtgaca gaagaaggaa ttgcttattt gtacaaagca
     1441 aaaacaaaag aagaacgaca agcagcaatt gcagcaattg gcggtgttac acctcttgga
     1501 atgactagca cgaaagaatc actagatagc ttgcgtgcag ccggcatcgt gaaattacca
     1561 gaagatctag gcatcaaacg tagtgaagcg aaacgttcat tattagctgc acaaaccatc
     1621 gatgatttga tcgaatggtc agatggcctg tatgaaccac caatgaaatt cagaacttgg
     1681 tcataaaatt ggcgagaaaa accttttgtc gccatttacc cagatggaag aagaaagacg
     1741 aataagcaga acgtgtatgc tctgttttac aaaaagggga gagatccatg gaacaattag
     1801 cttataaata tccaacgaaa cagccgatca ataagaaaat ccatgtagga gtggtcggtt
     1861 caggagattt ggaaatcttg atgtttccaa cagaaaaaac agaatctact gtaaaaattt
     1921 gtacaggaag cgatggcttc ggcgaagtat ggaacaatgt attaacgaga ttttttgatc
     1981 gttatccaat ttcagcagat atcgtgatca atgacttcgg tgctacacca ggagttgtgt
     2041 acctacgatt gacgcaagca atggaggaac tttcagatga aaaatagttt tgtagaatta
     2101 aacgccagag aacgagtgaa cgctctactt gacaatggac gtgaattgat tgatccattc
     2161 gatcaaatca tcgcgccaag tttaccagca caaggaatcg tccctgaaag tgatgacggg
     2221 atcgttgtca gccgtggcac gatcaatggc aaacaagcag tagtaatcgc tatggaagga
     2281 aaattccaag gtggcggtat cggtgaagtt tccggtgcaa aaatcgttgc tgcacttgaa
     2341 catgtgttag aagaaaataa aaaaggaaat gagatctttc cagtcattgt actagataca
     2401 ggtggtgttc gtttgcaaga agcaaactac ggactgttat ctatttcaga aatcggaaat
     2461 gtaatcattg ctctaaaaca atatgtacca gtcatcggtt tgattccagg acgtgtagga
     2521 tcatttggcg gtatgtcgat tacttcagca ttaatgagtt atttaatcgc aacaaaaaaa
     2581 gcgagagtcg gtttgaatgg accagaagtt attgaacaag aagcaggcgt tcgagaattt
     2641 gattctagtg acaaagactt gatttggaat acattaggat ctcgtcaacg ggtagaatca
     2701 aaaatcattg atgaattagt gactgatagt gtagaagcaa tcaaagctgc tgtcatcgca
     2761 gcaatggacg aacgaaaaga cacgcatcgc agtgaaagct cagcatttta tttatcatta
     2821 cttgattctt tagatctttc aaaaccaatg gccattgacg attacaatga agcaattgct
     2881 aaccatcaag caaaatcaat tgacttacca gaagtagcag aaggaaatga accagctgca
     2941 gcaagtgttg gttacgattg gtttgcggca ctaactggca tgaaaaatcc aacttcttct
     3001 attagtactg tgtattatgg taaatcaagt aaattcatag aagaagccat cgtgacatca
     3061 atcgtacctg atcctaaaaa cccgatgtac cgtgtacgaa acggtgaagt tggtttagtt
     3121 gaaggtttcc gcatgggaca aattttggat cacgtttacg aagaagataa agacaaaaaa
     3181 gtgaaacggc caatcatcgt tgtgatcgat gtaccaagcc aagcatacgg ctataacgaa
     3241 gaattgatcg gtatccatgt ggctttggct aatagtgctg ctgcttatgc aaaactccgt
     3301 caagctggac atccagttat tggattgatt gcaggaaatg ctatttcagg tgcattttta
     3361 gcacatggat tacaatcaag ccgcttgatt gctttaaaca ataaagaaat taccgtccaa
     3421 gcgatgtcta aagaaagtgc tgcacgtgtg acaaaacgtt cggttgctga aatcgaagca
     3481 gctgctgaaa aagttccagc tatcgcttac gatatcaata attatacaaa actaggagcg
     3541 atctatcatt tcctagaaga tatcaccgac caagctgctt cagaacaaaa tgtcagcaaa
     3601 gtcatcgaag caatcaatga agccattcaa gatgtccgca atacaaatga cacgatgctg
     3661 acaaatcgat acacaaacga aacagccaaa acattcggac gtgtcgaaac aaataaagtc
     3721 cgtgccaaaa tggacgaaga atgggatgca taaaaaatga ttcaagcaca tgatattgtc
     3781 ctttttgata aacatgaaat agaagggttc accttgcctg aatgggtcgt tctaactgaa
     3841 aagatgttcg gaacagttcg tcgtgcgtcg acggatcacg cagaaatact accgattggt
     3901 ttacgtggac ctgcccgcaa tcagagatgg tcaacagaaa tctcaaaaca tgccgtcaaa
     3961 caagtcattc atccgtggga catcatcgaa caagaatcgt atagacaaga gaaaatcatg
     4021 gattatcctg tttatcagca atttcatgct gctagaaaca tattatcagg atgtaaatgg
     4081 ggaatcggcg gcagcttagg atttgaatta tcgactggga ttccggcagt aaaagaaacc
     4141 agtgattttg atttgctgct gtacgcaaat tcaccgattg aattgccaat tcaggtaatt
     4201 caatcgcatc ctgctttttt tgagcaattc gatacccaag tagtgactcg gcaaggcggt
     4261 ttttcactaa aagagtattt gagagagcca gagaaaaaaa ttctattgaa aacagtcgaa
     4321 ggacctaaat taaccaaaga actttggtaa taaagaaaaa gagagagaaa gagatgtttg
     4381 aaaacaaaaa aatcaaaatc aacgtaagtg acttccagaa agtatcagct gtaacggctg
     4441 ttatgatgca aacgatattg agctttgctt tggcaaacag ccacagcgca acaaatacag
     4501 cttttcttgg taccttttac gtcatcgtta aatacacagc accaatgttt atttttgcaa
     4561 ttgtttataa tatggtaaaa acaagtcagc atttgtctta ttgggaattt ttgaaagaaa
     4621 agttttttga actagtcgtt ccgtatcttc tatggacgac ggcttatctt tggctatttc
     4681 cagcagtcca acaacaaact ccgtatacgg atactgcaag ctttttactg aaatatgtca
     4741 caggtgatgg agcgcctcat ttatggtata ccgttatgat gctgcagatc caattgttca
     4801 tgccgttttt tgtttggctc ggttacaaag tatttgctaa taaaaaatat gtatggccag
     4861 tattgattgg ttcaacgatt ttatatgtcg catggtatct gttctatcaa acacaagtat
     4921 tgactgggcc ttatcatgac agctggtact tattagatcg gtttgttgct agtttcatga
     4981 tttatggggt agccgcattt gtttatcacg aaaaagtcta tcaatatttg gacagagtac
     5041 gttatctttt cttacctgtt gccttagtta tcgcgttctt ttctgtccgt tcattgctcg
     5101 cacatccagg agacttgagc tttgcgaatg caccttactt gaatacgatc caaagtttat
     5161 acagtttagt catcatcttt gctgtcttca taggcgcttc caaaatgatt gtaaacgatt
     5221 cgccaaaatt gccattgttc aaatggttgt ctgtttatgc ttatcgtacg tatttagcca
     5281 atgtatttgt tttccaagta ctgttattat tgtttaaaga tagttggctg cagttacctt
     5341 caggagtcat gattcttgta gcttatctaa tgacggcaag ttgtgcattt gctttaagct
     5401 ggttacttca tatcatttgg gtagctatca aaaaaggttt cagtaagtaa tagtaggaag
     5461 aaggagtgag tgtcatgata aatgacgaac gaaaaaaagc agtgactcag caactcgctg
     5521 cgttggtaga ggaagcattg atcgcagaag taacattaag tccaaaacct ggattagtgg
     5581 atgcactgga tgcgggtgca cattccgata tggactatgc tttatttctt aagtctgcta
     5641 agacgctaac tccttttttt gaagaaatgg ctcaaatagc atggcatcat gctattgatc
     5701 aggagttaag agaaagaatt gctgagattg ggcgaaaagc ggaaaaggca atgctgattg
     5761 ctacaaatgg catcaatacc cataaaggag cgatttgggc aatggggcta ttgatcagtg
     5821 tcattgcaca aaaaatcagt atggaacagc ctttttcttt atcgaaaata cttgatgatg
     5881 tcagcaagct tgctgctttt cctgatacaa aatatcgtca taaaaaaacc actcatggtc
     5941 atagggtgaa gcaaaaatat ggcgtaaatg gtgcatatga agaagcgata ttagggtatc
     6001 cgcatatcag gcttgcaatt caagcagcta aggagctaga taatgattca tatgtacaga
     6061 aacaaatgca tatgttgtta gttttgatga gttcgctaga tgacacatgc gtattgcatc
     6121 gcagtgatca gaaaacgtta gtggaaatgc aaaaacttgc tcatgctgct agtcaaagtg
     6181 ctttaccaaa tcgtccattt acagcactgt tgcacttttg tcaggaaaac caaatctctc
     6241 ctggaggaag cgcagatctc ttgtctgcaa gcttgttcat gctgaagata gaggaattat
     6301 ggactgccga cacatacatt tgtccgatgt tactcaatga ctaaataaga caaaattgct
     6361 aaaaaaacag tttatgcatc tggttattga ctcttccttt tagcaaggct atcataaaaa
     6421 tagttttttg taataaaaga taacgaggaa gacaatgaaa aaaatcaatt atttagcaat
     6481 cattttgtta gtgggcctat gtttgtttgc tctgccgacc caaggattcg ctgtcgcggg
     6541 tggtcatggt ggaggtacat ctagcggtcg aagttcgggc agaggttcat ttggtggtcg
     6601 ttcatatggg ggcagttatt ttggcagtcg ttcttacggc agagattctt atgggtatta
     6661 tggtccaggt acaggtcctt ctagttatga gcaaacggga tttcttgcat tagctgtttt
     6721 gtcagtagga ggggtttctg ctttttctat cgtcaagaat aaaagacaaa agaaaaggct
     6781 gaaagcggag atattgtccc gcatgtcagg aacggctaaa gaaaagaaag cacagtttct
     6841 ggaaatccaa aagatattca tgaagatcca atccgcttgg gaagaagaaa ctcctgaaaa
     6901 agccagaagc tgttatacag acaagctgtt tgccgaacat cagaaagttc tgcaaaaaaa
     6961 caaagcagac ggtgtaagaa atcatactaa aaaaataact attcaggaag taggaaatta
     7021 tcgacagatc cacgaaaata gttttgccat ccgaattgat ttcagatgct gtgattattc
     7081 agtcgatcga aaagatgggc gattgatctc aggaagtaag acacaacgac agtatttttc
     7141 tcagatatgg tattttgatt tttctgagaa agaaaaaaga tggcaagccg attttattca
     7201 gccgattatg ctggattgac aggaaatatt tatgaacgga gttgtgacaa aagccttgtc
     7261 gcagctcctt tttctgaata aacggtgtgc cagagatagc ctatcggcaa taaggcacga
     7321 ttttgtaaaa tatgggaagc gatttttcaa aatcgcttct tatcgctcaa ggctgaacat
     7381 ctctgtcaca acctctccac taaatggtgt ttaaaagctt gcatctgcga taataagaac
     7441 aaccaaacga aaaataggaa gcactatttt agtttagttg ttcttattac ttgatgcagg
     7501 acagcttttt cacaacctct tttttgtata gatgggcaaa atttttgtaa taataggcgt
     7561 atgtcttttt cgttgaggag atgaaaaagc atatggctta ttttttgttt gctttgctta
     7621 ctttctttcc acttcaagtg gcagaaatat ctacttttta ttatgtaagc ggaaccatat
     7681 acttgaaatc agaaggagat catttctatg aaaacaagaa caattgaaat acttcacaga
     7741 atcatgacag cccaatatcc agtaaaagca aaacatctag caagtgagtt caatgtcagt
     7801 cagcggacga tccgtcagga agttcttgaa gcgaatatct ggttgagcag taaaaagctt
     7861 ccggagatcc gtacaattcg caacaaaggt tttttgttga agctggaaaa ggaaaagcaa
     7921 gctcgattag aaactgaaat aaaagatgtg aaagacggct ttttgaatcg gcgcgaacgt
     7981 atttttgatc tagttttagc cgttgcttac agccgaatcc cgatccatct aaacaaaaaa
     8041 gaagaagaat ttcaaatctc taaaagtaca ttagatgaag atatgagacg gttacgagca
     8101 acactggtca gttatgggat agagatcgtc agttatggaa agcaaggact ggtttataaa
     8161 ggttcagaac gatctatccg tacgatgatc tatgatttta tcaatcagaa tctgggaatg
     8221 accgacttct ttatgaatca agatgcacaa ttaacaccgg ctcaaaaaat ctttctccat
     8281 tattttcctt tggtggaaat caaaaaaata caggagatct attccaataa gttgttgaaa
     8341 caagaagatg atatttataa aaaacaaatc ctgcttttta cattgatctg gatccagcga
     8401 atcaggaaac atgaatttat ctctgccatt aattggaaaa atacagaaat aaaagacagt
     8461 agcttttctg catttattga ccacatgatc gaggaatttc aattggacaa tgtcccgcaa
     8521 gtagaaagaa attatcttcg tttcactatt gagacgttca atgctagaga tatctccaat
     8581 tcaatcgagt gggtacaagc tcaattacta acgatccaat tgatccaatt tgtagagaac
     8641 gagacacacg tacctttcca tctaaaggaa gaagctttat tcgaaaatct ctataagcac
     8701 atggctgcac tgattgtaag aatcaagaat catatccaag tcatgaatcc attgaaagaa
     8761 aacatccgaa caaattattt cccaatctac cgcgcagtca ctcattttgt tcctacgatc
     8821 gaagaagtaa ttggtggcaa gatactggag gacgagatag cttttcttgt catccacttt
     8881 tcgacgatcg ccagtgtaat caagcgagac ttgaattatg tttataaatc agtcgttgtc
     8941 tgtaaccatg ggatggctac tggaaatctg ttagcagaga atctgaaaga aaagtttcct
     9001 cagatcgaga ttactgcagt tttaagttca aaagaaatcg aattagtaga taaattggat
     9061 gtcaatctgg ttttcagcac ctttcaattg aattatcgaa ataagccgct tttagttgtc
     9121 gatccgattt taactgagac gaatatcttg gttatagaag actttttgaa aagaaatgaa
     9181 gaactacagc ggttagagcc taacagtcat gaatcgacgg agttattcac tcaaataata
     9241 gatgtcatga aagaaagtgg tggccgagta gatcgaatca tctatggcaa gttagaaaaa
     9301 ttattcgaaa caaacaattt agaaatcaac aaaagggaga tccagcctat gttaaaagat
     9361 attttgacgg acagcaatat catgatcaaa gaaaaagcca ctacttggga ggaatcgatt
     9421 gaactagctg ctagaccatt ggttaaagaa aacattgtcg aagagcgcta tgtcgaagcg
     9481 atgattcgtg cagtgaatga ttacggaccg tatatcgtga ttggcaaaca catggcacta
     9541 gcccatgccc gaccagaaga tggggtcaat aaattaggag tcagcgtagc tacgattgag
     9601 caaccaatcg atttcggtaa tcctgaaatg gatccagtaa agattatttt ttgtttggca
     9661 gcagtagact cctactcgca tctgaacatc atgaaagaac taattgaatt gatcaacaac
     9721 gaagagaagt tggatcgatt gattgaatgc acaaatatcg aagatttcaa acaactgctt
     9781 ttttcagtag ttgagaaata ataaatgata cgggggagaa caaagatgaa caaattgaac
     9841 attttattcg tatgcggtgc aggtttagga agtagttttg cagcacagat gtctgctgag
     9901 gatgttttga acaaattagg agtagaagcc aaactggacc attgtgatat ttcttccgca
     9961 gtttccatga aaccagatgt gatcatcaca gcagaaaatt ttcgttcgca gttcgaaaag
    10021 ttcgcagttg atcctaaaac ggcgatgatc tatttgaaaa atattgtttc aaaaaatgaa
    10081 attgaagaaa aaatcacccc agttctgcaa gataagggag ttatctaaat acaaaaagaa
    10141 aagagggtaa aaactgaa
//
LOCUS       DAFGFZ010000102        10036 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000102 DAFGFZ010000000
VERSION     DAFGFZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.104"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            21..527
                     /locus_tag="LR726_002290"
     CDS             21..527
                     /locus_tag="LR726_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL6217335.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            544..1443
                     /locus_tag="LR726_002291"
     CDS             544..1443
                     /locus_tag="LR726_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL6217336.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1530..2414
                     /locus_tag="LR726_002292"
     CDS             1530..2414
                     /locus_tag="LR726_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL6217337.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2606..2677)
                     /locus_tag="LR726_002293"
     tRNA            complement(2606..2677)
                     /locus_tag="LR726_002293"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2642..2644),aa:Arg,seq:ccg)
     gene            2737..4071
                     /gene="rpoN"
                     /locus_tag="LR726_002294"
     CDS             2737..4071
                     /gene="rpoN"
                     /locus_tag="LR726_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL6217338.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4220..5311
                     /locus_tag="LR726_002295"
     CDS             4220..5311
                     /locus_tag="LR726_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL6217339.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5352..6353
                     /gene="gap"
                     /locus_tag="LR726_002296"
     CDS             5352..6353
                     /gene="gap"
                     /locus_tag="LR726_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL6217340.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6463..7653
                     /locus_tag="LR726_002297"
     CDS             6463..7653
                     /locus_tag="LR726_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL6217341.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7757..8512
                     /locus_tag="LR726_002298"
     CDS             7757..8512
                     /locus_tag="LR726_002298"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL6217342.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8628..9926
                     /gene="eno"
                     /locus_tag="LR726_002299"
     CDS             8628..9926
                     /gene="eno"
                     /locus_tag="LR726_002299"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL6217343.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3509 a         1853 c         2091 g         2583 t
ORIGIN      
        1 gaggcagaaa ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa
       61 aataccgtca cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca
      121 cgatgaatta cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata
      181 aagccctgaa ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc
      241 ggatattgat ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact
      301 tatttgaaat tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc
      361 atatgatttt agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg
      421 aaggatttcg tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa
      481 cgaaaatgta ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt
      541 gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca
      601 attcccaaag ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt
      661 ataaaagcaa agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta
      721 atgattacaa caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta
      781 cccatcttag gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa
      841 aaaatcgata ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt
      901 catggattaa atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa
      961 gaatatggaa attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta
     1021 gacgataacc ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat
     1081 gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt
     1141 ttattcaaag atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc
     1201 gaacaagtca aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg
     1261 acgatagaaa aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat
     1321 caattagttt tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa
     1381 tttgtcattc ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat
     1441 tagacgagaa gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata
     1501 agaagagaaa aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta
     1561 ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag
     1621 taaaaggaca aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat
     1681 caaccattaa aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag
     1741 cggaaaaggt acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag
     1801 atccggctgt caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac
     1861 cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag
     1921 aaaatacggc gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg
     1981 gcaatcacac atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg
     2041 aagtgacgaa agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc
     2101 gtccgcccta tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg
     2161 aatggtctgt tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga
     2221 tccaagcaac agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga
     2281 ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag
     2341 ttggggattt attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc
     2401 ggccagttca gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg
     2461 aacacataac taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac
     2521 tgtccaccaa ttttctttgg cggacagttt tcacttaaaa ataaagaagc aactattcgt
     2581 aaattatgct aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa
     2641 ccggaatctt acgtgatatc cactacacta aggacgcaag cactgggata attgtaacta
     2701 aacagataaa aaattacaag gggattggac aaaaaaatga aatttgagca aaatttttca
     2761 caaaaacagc agcagacaca gaaactagcc atgacgcaac agttgcaaca gtccatccaa
     2821 gttttacaat ataacagtga ggagctgttg gcattcgttg agaatcaagc aatggaaaat
     2881 ccattggtgg aagttgttga gccggaatgg cagccagatt acataaaagc ttcttcatct
     2941 tcttatgaag gagaagagac aaattaccta aatcagattc ctgatacaaa agggtcttta
     3001 tttgattcat tgatcgaaca agtccatttg aattaccgag atactttttt gaggaaaatc
     3061 gttctttatc ttgtagaata catcgatttg aacggatttt tgacgattag tttagaagaa
     3121 gccggaaaag agattggagc tacgcctatc caaatgctcg atgcgttgac tctgattcag
     3181 caattagaac cagctggagt aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca
     3241 gaacgagatg attatgcgcc ggaattagct tatatcgtgt tggaagaatg ttttgaggaa
     3301 ctggtggagc gaaaatggaa agaaatcgca caacgttttt ctgttgatct acatgctgtt
     3361 cagcagatat tcgactattt acaaacgttg agtccaagcc ctggccggat tttcgaccgt
     3421 agcagcgaat tgtacattat tcctgatgtc cgggtattag tggacgacga taaaaatgta
     3481 caagttatct caaaccgaaa aaatcagcca aatatacgct ttcaagagag ttacttcaaa
     3541 caaatgtcac aacaagcaga taaagaaact gaaaattatt taaaagaacg gaaacaggaa
     3601 ttcgaatggt tgaagaaaac gatcctacaa cgtggggata caattttgcg ggttgctcaa
     3661 gtcattgttt cccggcaaaa agctttcttt atcgacaagg aacgtccaat caagcctctt
     3721 acgttaaagg aagttgcaac tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc
     3781 aacgggaaat atttggaaac agacttcggt gtatttgaat taaaaaaatt tttcactaca
     3841 cgaattccta cgaatagtac agaacagaca gaagaccttt cagcagatac agcgaagaaa
     3901 aagctgcagg aattagtcga tcaagaagac aaaaacaagc cgttgtctga ccagaaattg
     3961 gttgaattgc tgaaaaaaga tgagatagcg atctcaagac gaacagtagc aaaatatcgt
     4021 gatttattag gaatcccgag ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa
     4081 agactagaaa aagtatttca ctttttctag tctttttagt tgaaaaacga gagcaaaact
     4141 gttagactta cagtcgtgag gttgagattt ccctttattg atgtgaaatt cgaatagtta
     4201 atttcttttt ttatcgtata tgggtcgttt taagtctaca ttggacgtaa agtgtccaac
     4261 taaggagatc gctatgctgg atgaaataaa aatgattgaa tctgttgcac cagatattat
     4321 cgaagtgttg caagaacgct ataaaatttt acgaaatatc tattggatgc agccgattgg
     4381 acgtcgaagt ttatcggaaa gtatggggat cacagaacga gtattgcgaa cagaaacaga
     4441 tcttttgaaa aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa
     4501 gggcgaagaa gtttatcaaa gccttgaaaa cttcatggat cagcttttag gtacgcatca
     4561 aaccgaacaa cagttggcag aatattttgg taaccaacgc tgtatcgttg tttcaggaaa
     4621 cagcgaagag cagaccaaag tggctgatgc attcggcgaa gcgctgagcg aagccctaga
     4681 ccgactactt ccagaaggcg agaatatcat cgctgtgatg ggaggaacta ctatggctgt
     4741 ggtagccgaa caattgtcta atctggaaaa taagaaacgg cataacctgt ttgtccctgc
     4801 aagaggcggg attggtgaag cgataactgt acaggcaaat tctgtcagtg caagaatggc
     4861 tgctaaagca aacggtaacc accgtgcact ttatgttccg gaacaattaa gtctggcaac
     4921 ttacaattct ttactcaatg agccatctat ccaagaagtc ttaaacttga ttagtgaagc
     4981 gaattgtgtt attcatagta ttgggcgtgc attacacatg gcagcacgtc gtaagatgac
     5041 tgaaaaagaa ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt ttggctactt
     5101 tttcaatgaa aaaggtgaag tcgtttataa ggtccctaga atcggcatag aattgcaaga
     5161 tttggaaaaa gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat
     5221 tcgagcttat atgaaaaatg caccaaaaca aacgtggctc atcactgatg aagctgctgc
     5281 aaatgagatt ttaaaagggg taacccttta aaataaatat ttttttgatt ttcataagga
     5341 ggaaatctta aatgacagtt aaagtaggta ttaatggttt tggacgtatt ggacgtctag
     5401 cattccgtcg tatccaagat gtagatggaa tcgaagtagt agcaatcaac gacttgacag
     5461 atgctaaaat gttggcacac ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa
     5521 cagttgaagt tcatgaaggt tcattcaacg taaacggaaa agaagttaaa gtattagcta
     5581 accgcaaccc agaagaatta ccatggggcg aactaggcgt agatatcgtt ctagaatgta
     5641 ctggtttctt cacttctaaa tcagcagctg aaaaacattt aacagctggt gctaaacgcg
     5701 ttgttatctc tgctcctggc ggaaacgatg ttccaacaat cgtttataac acaaaccacg
     5761 aaacattaac tggtaaagaa acagttatct caggtgcttc atgtactact aactgtttag
     5821 ctcctatggc taaaacattg aacgacaaat ttggtgttgt tgaaggatta atgacaacta
     5881 tccacgctta cacaggtgac caaatgactc tagacggacc tcatcctaaa ggtgacttcc
     5941 gccgtgcacg cgctgctgct gcaaacatcg ttcctaactc aacaggtgct gctaaagcta
     6001 tcggtttagt aattcctgaa ttgaacggta aattagacgg agctgctcaa cgtgttcctg
     6061 taccaacagg ttcattaaca gaattagtaa cagttcttga aaaagaagta actgttgacg
     6121 aaatcaatgc agcaatgaaa gaagcttcaa acgaatctta cggatacaac acagacgaaa
     6181 tcgtttcttc agatatcgtt ggtatgactt acggttcatt atttgatgct acacaaacta
     6241 aagtaatgac agttggcgac aaacaattag ttaaaactgt tgcttggtac gacaacgaaa
     6301 tgtcatacac tgcacaatta gtacgtactt tagaatactt cgctaactta taagatagac
     6361 aaacgtacaa gtgtaaatta tagacatgta catttaaaag cggggaagca atcgcgcttc
     6421 ttccgctttt tttgataata agtttattca ggaggaaata aaatggctaa gaaaacagtg
     6481 aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg
     6541 aaagacggcg tgatcactga cgacactcgt atcaaagcag cattgccaac aatcaactac
     6601 gttctagaac aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa
     6661 gaagataaag aaggaaaatc tttagctcca gttgcaaaac gtctaggcga attattaggt
     6721 aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat tagaagaagc aatccgcaac
     6781 atgaatgacg gcgacgttgt cgtattcgaa aatacacgat ttgaagatat tgacggtaaa
     6841 aaagaaagca aaaatgatac agaactaggt aaatactggg cttcattagg cgatgtattt
     6901 gttaacgatg ctttcggtac agctcaccgt gcacatgctt ctaacgtagg tatcgcttca
     6961 actggtatcc caacagttgc tggattcttg atggaaaaag aaatcaagtt catcggtgaa
     7021 gcagtagaag aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa agtttctgat
     7081 aaaatcggcg taatcgaaaa cttgattcct aaagcagata aaatcttgat cggtggcgga
     7141 atgacttaca cattctacga agcaaaagga atcaagatcg gtaactctct agtagaagct
     7201 gataaagtgg aactagcaaa agaattaatc gaaaaagcag gcgacaaact tgtcttgcca
     7261 atcgacaatg tctgtgcacc agaattttca aatgatgttg aaacacaagt aatcgaagga
     7321 gacattcctg atggtttgat ggctttagac atcggcccag catcagtaaa attatttgct
     7381 gatacattac aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt atttgaaatg
     7441 agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca
     7501 acaacaatca tcggtggcgg agactcagct gcagcagcag aacaattagg atttgctgac
     7561 aaattcacac acatctcaac aggtggtggc gcaagcttgg aattgcttga aggtaaagaa
     7621 ttaccaggac tagcagcaat caacgataaa taaacgacca agtttgctca attctgacga
     7681 gtaatcgatc aaaaggtgaa gggagttttc atgactccct agccattttt caataaatta
     7741 aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac
     7801 tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa
     7861 agttgattca gtcatcggat ctcctgcatt attcttacaa gaattaatga catcagctga
     7921 aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac
     7981 tggtgaaact tcaccagcag ctcttgctga tctaggtgtt cattatgtga tcatcggaca
     8041 ttcagaacgt cgtgaatatt tccatgaaac agacgaagac atcaataaaa aagcaaaagc
     8101 tatctttgca aatggtatga caccaatcct atgctgcggc gaatctttag aaacctatga
     8161 agcaggcaaa actgctgaat ggatcgaagg acaaattaca gctggtttag ctgatctttc
     8221 agctgaacaa gtgtcaaaca tggttattgc ttatgaacca atctgggcaa tcggtactgg
     8281 taaatcagct gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc aaacagttga
     8341 aaaactttat ggaaaagaag tatcagaagc agttcgtatc caatacggcg gttctgtaaa
     8401 accagaaaat attgctgaat acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg
     8461 cgcaagcttg caagcagatt catttttgtc attgttagac gctgtaaaat aattgttgaa
     8521 aatttgcgaa aaatgcaaga aatgtttgca ctaatgagca aaattaacta aaatcgtata
     8581 tgagggaaat cccttaggaa aaaacaaact caaaggagag acaaaacatg tcaattatta
     8641 ctgatgttta cgctcgcgag atcttagact cacgtggtaa cccaacaatc gaagtagaag
     8701 tatacacaga aagcggagct tttggccgcg gaatggttcc ttcaggtgct tcaactggtg
     8761 aatacgaagc agttgaatta cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa
     8821 ctaaagctgt cgacaacgta aacaacatca ttgctgaagc aatcattggc tacgatgttc
     8881 gtgaccaaat ggctatcgac aaagctatga tcgctttaga cggaactcct aacaaaggta
     8941 aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact
     9001 acctagaagt acctttatac cactacctag gcggattcaa cacaaaagta ttgccaacac
     9061 caatgatgaa catcatcaac ggcggatcac atgctgataa ctcaatcgac ttccaagaat
     9121 tcatgatcat gcctgttggc gctccaacat tcaaagaagc tctacgtatg ggtgctgaag
     9181 tattccacgc attagcttca atcttgaaag ctcgcggatt agcaacttct gttggtgacg
     9241 aaggtggatt tgctccaaac cttggttcaa acgaagaagg ttttgaagta atcatcgaag
     9301 caatcgaaaa agctggctat gtacctggta aagacgttgt tcttgctatg gatgctgctt
     9361 cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact agctgactca ggcgaaggcg
     9421 aaaaaacaac tgacgaaatg atcaaattct acgaagaatt agtttctaaa tatccaatca
     9481 tctcaatcga agatggatta gacgaaaacg actgggacgg attcaagaaa ttaactgacg
     9541 tattaggcga caaagttcaa ttagttggtg acgacttgtt cgtaacaaac actcaaaaat
     9601 tatctgaagg tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg aaccaaatcg
     9661 gtacattaac agaaactttc gaagctatcg aaatggctaa agaagctggc tacacagcag
     9721 ttgtatctca ccgttctggt gaaacagaag attcaacaat ctctgatatc gctgttgcaa
     9781 caaatgctgg ccaaatcaaa actggttctc tatcacgtac tgaccgtatt gctaaataca
     9841 accaattact acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat
     9901 ctttctacaa cttaaaaaac aaataattct tttctaatga agatacatga tcgactattg
     9961 aaagatagtc gagtatgatc acttagttaa gtaatcgaac tctctacttg ctgaaatatc
    10021 agcaggtaga gagttt
//
LOCUS       DAFGFZ010000103        10032 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000103 DAFGFZ010000000
VERSION     DAFGFZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.105"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(46..366)
                     /locus_tag="LR726_002300"
     CDS             complement(46..366)
                     /locus_tag="LR726_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL6217344.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="LR726_002301"
     CDS             complement(514..2748)
                     /locus_tag="LR726_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6217345.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="LR726_002302"
     CDS             3227..3493
                     /locus_tag="LR726_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL6217346.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="LR726_002303"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="LR726_002303"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL6217347.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="LR726_002304"
     CDS             5384..6430
                     /locus_tag="LR726_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL6217348.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="LR726_002305"
     CDS             6447..7670
                     /locus_tag="LR726_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL6217349.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="LR726_002306"
     CDS             7759..8646
                     /locus_tag="LR726_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL6217350.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="LR726_002307"
     CDS             8732..8962
                     /locus_tag="LR726_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL6217351.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="LR726_002308"
     CDS             complement(9019..9984)
                     /locus_tag="LR726_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL6217352.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3190 a         1778 c         2028 g         3036 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aa
//
LOCUS       DAFGFZ010000104        10021 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000104 DAFGFZ010000000
VERSION     DAFGFZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.106"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(487..711)
                     /locus_tag="LR726_002309"
     CDS             complement(487..711)
                     /locus_tag="LR726_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217353.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            902..3679
                     /locus_tag="LR726_002310"
     CDS             902..3679
                     /locus_tag="LR726_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL6217354.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3760..4179
                     /locus_tag="LR726_002311"
     CDS             3760..4179
                     /locus_tag="LR726_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL6217355.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4191..4661
                     /locus_tag="LR726_002312"
     CDS             4191..4661
                     /locus_tag="LR726_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217356.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4678..5457
                     /locus_tag="LR726_002313"
     CDS             4678..5457
                     /locus_tag="LR726_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217357.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5444..6265
                     /locus_tag="LR726_002314"
     CDS             5444..6265
                     /locus_tag="LR726_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL6217358.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6282..7331
                     /locus_tag="LR726_002315"
     CDS             6282..7331
                     /locus_tag="LR726_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL6217359.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7344..8348
                     /locus_tag="LR726_002316"
     CDS             7344..8348
                     /locus_tag="LR726_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL6217360.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8981..10000
                     /locus_tag="LR726_002317"
     CDS             8981..10000
                     /locus_tag="LR726_002317"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL6217361.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1651 c         2093 g         2915 t
ORIGIN      
        1 ttaatttgtt cttgcaatct tttttgctac gtaatttttg taaaaaagtg ggttgagaga
       61 aatctaattg attttcaaag aaggagtatg aaaatgagaa aaatatttat tcgtttattg
      121 tgggtactat cttagaggaa taaataatag tggagaaaac tatggtttgg cgcagattct
      181 atcaggtttg atggaacgaa gacaacctcc cgacatcaag tcaaaagcta cttgagattt
      241 tgatctgaaa ataaaaaaag aaaacaaggt aggaacatca atgtttctac cttgttttcg
      301 taatcaatgg gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca
      361 gtacctcttt actctctgaa agcatcataa ctcgtttttc ctcattatgc caataaattg
      421 ttgttatacc acatataagg atttgtaata gctaaaaaca gacaggaaag ttgaaatctg
      481 tgaatcttat tttgtatgga aggatttata gaacgcaaaa atcaagataa agatgaataa
      541 aaatcctgtt tcggctatat attgcgtatc tacgccaata aaacgagttt gttctgtcat
      601 agccatataa agcaaaggca aaaatacaac tacagcgaga taagatccga tgatttctcg
      661 aaaagttttc caggaagtat gtttcctgtt tttatattta tccgatttca ttgaatagtc
      721 atagcctcct tattctttca tatgttgaaa ttccagtata aaaactggaa ggaactgtac
      781 gttttttaga ataaaaatta taagggataa gtaggctgct gtctataata agatatattt
      841 ttcaattctt tattaaagat tttattgaca aaatgaatcc gttttctatg atggaggagg
      901 ggtgaataag ttgttgaaga aggaaatttt atgttactta gagaaccaga cagccttttt
      961 cgatttagaa catatgaacg aggtctttac agcacaaagg ttagctgatc attttggggt
     1021 aaaaagaaac acagtcagtc attatttgaa ccagttaaat gatgagcaac tactagtcaa
     1081 aatcaattct cgaccagtga tttatttcca caaagaagct tttgaaaaac aattttttaa
     1141 gttacgcacg aatttttatt tttctatcga tgatctcaaa gaggaacagc cttttttcgc
     1201 gcaaccaaaa gacttattct cttttatgat cggatacgac gccagtttga aagaaagtat
     1261 cgaacaaatc aaaacagctc tttactatcc agatggcgga ttaccacttt tgattacagg
     1321 cgaaagtggc acaggtaaaa gttatttagt taatttggtc tatcagtatt gtctgcttca
     1381 tgatctgcta gaagattctg caccattcat cacttttaat tgtgcacaat atgcagataa
     1441 tcctgaactt ttgactagca atttgtttgg acatgttaag ggagcgtaca ctggtgcaga
     1501 agaaaataaa aaaggggctt ttgaagcagc agatggtggg atcttgtttt tagacgaggt
     1561 acatcgctta acaccagaag gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat
     1621 ttatcgtatg ggagaaccta atttgtcacg ccggatcaaa acacgtctct tttttgctac
     1681 gactgaagag attactagtc atttcttaac aactttcatt cgaagaatac ctatccgaat
     1741 cgaactacct gcacttaatc agcgtagtcg taatgaacgg ctagaactga tttactcttt
     1801 ttttctcgaa gaacaaagaa aaatggggag ccctttgtca gtcagtggac cagctttgtc
     1861 acttttaaca agtcagaaat tatccggaaa cataggagag ctgaaaaata tcgtcaaagt
     1921 ttcagcagca aaagcatatg ctgaacagcg tgaacagcct gaaatccatg tcacgatcca
     1981 tcatttgcaa aaagaactac ttgcgcagcc tattactaaa aatacagcac agcaaggaat
     2041 ctatattacg caagaccaga ccttagagca attaatggag aagcaacagc ccgaacagca
     2101 gcgaatcgtt aaaagttttg agcaaatcct aattgatttc aagaaaaata actgtctgct
     2161 atattcttgc gaaggaaaat taaagcaaga agtgattcaa ttatttgatt ttttgctgtt
     2221 tgagaccagt agacaagaaa aacacgagct gctggtttat tggacacaat ctatccgaga
     2281 gacctttcga caaatggaaa ctgcctatca aatcaaattc gatggaaaca gtgtctatgc
     2341 cttgagttac tatctctatc agcgaagcac tatcaaatgg ttaccggaag aacatgatat
     2401 tatccaacta atcaaagaat tagaacaaca agtttctcag tcttatccat ctagttatca
     2461 ctatgtccaa cgaattctgg aattaagtaa gatcaaatta gatattgaag acacaagtat
     2521 ggatcggatt ttactgacga tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact
     2581 gccaaaagcg atgattgctg ctcacggtta tgcaactgct agcagtatgg caaatgtagt
     2641 aaatcgttta ttacaagagg atctatttga atcatttgat atgccactag atgtcacacc
     2701 acagcagatt gcttcagaaa tccttgatta ttgcgaaaac agcgatgttt caaatggatt
     2761 gatcatatta gtagacatgg gatctctgaa agaaatccat cagtttttta aaaaacagtt
     2821 atccgtaccg cttttgattt tgaataatgt gacgacacct ttagcaatca cagtagggga
     2881 atgcctgcaa aaaaatgtat cattagaaga gatagcagaa gagactgttc gacagatcca
     2941 gccagaatgg cgactgctct atccagagga aaacaaaccc aaagcactca ttactacttg
     3001 cttcactgga atcggtacgg cggtccactt aagtgaactg ctagaaaaaa gcttacctac
     3061 ggcttgtcag ttgaaaatca tcccatatga atatcagcaa ttaaaagata agaaaaacag
     3121 tgacccatta ttttccatat acgaagtcgt aggaatgatt ggcaccacag atcctgatat
     3181 caccaatatc gactatcttt cattagaaga cctcatctcg ggggaaaaga tgtctgtact
     3241 agctgaatgg ttggacacta ctatgaatgc ttcagagaag gaaatattca atcagcgcat
     3301 cattcgtaat ttttcattgg aaaaagtact ggattccgtg actattctag atacagagaa
     3361 ggtgatggga gaaatcgatc attttatgag agaattagag gtgcagttga atcgaagcat
     3421 cagcaatccc aaaaaattag cgttgtatgt ccatgtgagc tgtctcattg aacgattgat
     3481 ccgacaaatg ccaatcgaaa cctatcaagg attagagaaa tggaagcagt gtcaaaaaga
     3541 agggttatct gctattaaat ctgcgtttag tgtcattgaa gagcattata gtgtcatgat
     3601 ccccaattct gagattgcct atatctatga tattttgagt gaagatacag agtttctctc
     3661 gatagaagaa gaattttgaa cataagcaag ttgttgccac taattttggc atgcaacttg
     3721 cttttttagt atgtgtaagt gaaaagagga gatgaaaaag tggatagaga aattatctta
     3781 gcttcccatg gaaaatttgc ttcagggata ttagattcat tagagctgat ctatgggaaa
     3841 aatcattcta ttactgtatt cgattgctat actgatgaga attttgattt agcggaaaca
     3901 gtaaggcagc tttttatgag gtacaaagat aaagaaatca ttgttctgac ggatcttttt
     3961 ggcggaagtg tcaacaacga attcttacaa tatatcaatc gggatcatgt ctatctcgtt
     4021 gccggattga atttgccact attgattgag ctagtatctc ggttggacgc tgaagtagag
     4081 acaactgttt tgatccagca ggtgctaact gattcaaaaa aaatgatcca gttttgtaac
     4141 gagcggctca atcagaaaat agaagaagac gaattttagg aggaagaaaa atgattgtat
     4201 tgacaagagt cgaccatcgg ttattacacg gacaagtagc tttttcatgg acacaaacaa
     4261 taggcgcaga ctgtattttg attgctaatg atgatgtacc tacgaatgag atacgaaaga
     4321 caacgatcaa attagccaag ccacaaggag tcaaactagt gatcaaatca attgatgact
     4381 cgattgccgc gttgaaaagc ggggtgacag ataaatataa gttattcatc gtagtggaat
     4441 cgatcgagga tgcatataag ctggcagaag cgtatccaga aatcaaaacc atcaacttag
     4501 gagggatcaa ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat attttaccag
     4561 aagaggagga acgcttgaaa cgattaattg acaaacaaat agaagtagaa atccgtcaag
     4621 taccagcaga taaaaaagtg gatgtaagag aagttttgta agaaaggaag aacctcaatg
     4681 attgtacaag cagtcttatt aggaattgtt gcatttgtcg cacaatcaga atatgcacta
     4741 gggacatcgt tgctttctcg tccgattgtg acggggctgt ttacaggaat cgtgttaggt
     4801 gatgtaaaaa ctgggatcat tatgggcgca actttagagt tagcatttat cggatcgttc
     4861 tcagtaggtg cttcgattcc acctgatgtt gtgaccggtg ggattttagg gacggctttt
     4921 gctattacag ctggagccgg tacagaaacc gcgcttttac taggtttgcc gattgctacg
     4981 ttgacattga tattgaaaaa tatttactta gggcttttga ttccaattat gaaccataaa
     5041 gcagacaatt atgcagaaga aggaaattat aaaggagtcg agcatatgca cttgcttgcc
     5101 ggtatcggtc tttccttgat gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc
     5161 aatacgatcg gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta
     5221 gctacagggt tgattccagc tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa
     5281 caagttgcac catatttttt cttaggtttt gctatagctt cttacttaga gattcctgtg
     5341 accggagtag ctatttttgg tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc
     5401 cgtcaactac cacttcaaac aactgcaggg gaggtagaag aagatgaaga attctgagat
     5461 caaaaacagc aaaataacga aaaaagagct aaatcacgta ttttggcgtt cttttcaaat
     5521 ggagttttct tggaattatg aacgtcagat gaatatggct tatgtttatg caatgattcc
     5581 gattttgaaa aagttatatc agtcgaaaga agaaatggca gcagctttga aacggcatct
     5641 agagtttttt aacacgaccc cacatatcgt tacactgatt ttaggaatca cggctgcaat
     5701 ggaggaagaa aataaggatg atcctgaatt tgatgtgact gcgatcgata gtatcaaaac
     5761 atctttgatg ggacctcttg cagggttagg agattctttc ttttggggaa ctttgcgatt
     5821 gattgccaca ggtgtgggta cctctttagc catgcaagga aatattttag gccctatctt
     5881 gttcattttg attttcaata ttccgcacat tctgtttcgc tatctagcaa caggttgggg
     5941 atatcgctta ggaacgggtt ttttgaaaaa gatccaggca aacggtatga tggaaagttt
     6001 gacattaggc gcgtctatta ttggactgat ggtcgttgga gcaatgactg ccacgatgat
     6061 caccatcaat attcctttaa aaatcggttc aggagaaaat gctgtgactg ttcaaagtat
     6121 ttttgatgat atcgttccaa atatcttatg tcttggagca tttggcatag tgttttatct
     6181 attgaagaaa gaagtgaaac cattaacgat cttgatcgga ctagctttct ttggaatttt
     6241 tggttcgtgg atagggattt tttaaaaaag gagcagagaa aatgtttacg atgcaagatt
     6301 atatctatga agaaaaggaa gtactaagta cgatcttgaa aaagaatgat ttttcgacaa
     6361 gagaacatat gaaaaaaaca gtgaatttac tgatcttagc aacgggttca tcttataacg
     6421 cttgtttagc agcgaagcca gcattggaat cttatggtga tcttactgtc gatattcaag
     6481 aaccttttta ttttttcaat tatggaaaac tttctccttc aatcgatact gtgattgctg
     6541 tgtcacaaag cgggaaaagt gcttctactg ttgaagcagt aaaaatgata caaaaacaag
     6601 ggttacctgt tgttgctatc acaaatgacg tgcaaagtcc acttgcttta gaagctgcac
     6661 aaattattga tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa ggctattctg
     6721 cgactgtttt acagttacta ctcttaggga taggaattgg aataagtaaa aataaaatca
     6781 gcaaaaaaat agaacaagac tacatgcaac agttgcgcaa aataatcaac catttaccag
     6841 cgattattca aaaaacggaa gaattttttg atggatatca aagtttattt cgtttagcac
     6901 agcgtttcgt caccattgga tatggtccaa actggggaac agctaaagaa gcagagacga
     6961 aattgactga aacaatccgc gttccgtcac aaggatttga gttagaagct tatatgcacg
     7021 gcccatacct ggaggcagat gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa
     7081 ataaggaacg ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga gagactctca
     7141 ccattaccac aaaaaaagca agaaacgaga aaacacttgg attagcaata gaatgtgatg
     7201 aatatctgtc tgtattagcg cttgtcgttc cgtttcaact attcgcttat aaaaccgcag
     7261 tagctaaagg tattgacttg aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa
     7321 gtaaattata agaggtggaa atcatgctga aattcaatga agaagaacaa atcaaagcaa
     7381 taaaaggcgc actagcttta cgtccgcaag tagaagaaat catcgataaa ctttatgcgg
     7441 aaaaatttga cgctgtctat tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag
     7501 ctgtcactta tatgaatggg aaaagcaact tgcctgtctt tgtacagcat gctgcagaat
     7561 attacactac tggaaacaaa agattaacaa aagattctat cgttattttg tcctctgtta
     7621 ccggtacaac tcaagaagta gtgaaagcag tagaacaaat caagaaagta ggagcgacac
     7681 tgattggatt tatcgataaa gcgaacagta agttgtctca gctatgcgat tttgtggtca
     7741 cttatccagc tcctggaaca gaacaaatca aattttttat ggcagcagat cgattgatgt
     7801 atttgaatgg agaattcgaa gcttattcag aatactatga acagctcgaa cgttatttac
     7861 caacagggtt agtggaagca gagaaaaaag cagatgcatt tggcttatca tttgcagaaa
     7921 aacatcgacg tgatagtatg cattatttta ttggagcagg gaatcaatgg ggagctgttt
     7981 attcatatgc catgtgttac tgggaagaac aaagctggct tccatcgaaa tctatccatg
     8041 ctgccgagtt tcttcacggg acgttagaga tcgtagaaga aacaacccca gtcacattat
     8101 ttttaggcga agatgaacag agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa
     8161 tttgcagcaa ttatacattg atagacacaa aagactatcc agtagaaggt atcagtgaaa
     8221 aatatcgcgg acgtgttttg tctttcttat tgatgcatgt agtgacacaa cgcatcgatg
     8281 ctcatgtgga gcagttgaac tgccatccat tagagatacg ccgctactat cgtcagtttg
     8341 attattagga tagactggat aaaaagaata taaaaaataa agagagcagc gataactgct
     8401 ccctttattt ttttaaaata ttggcatcaa ttacgttata aaacacattt tttgcattat
     8461 ctacagttca acttataatc tgtttaatgg gacatgtggg tgccaaccat tttttgttat
     8521 gcaatagtac tatttagttg ttgcatggat ccgtagtaaa agtccaagta caacgagttg
     8581 cgccagttta catagcgttt tgtatccaga atgttgacta aaacaaaagt tcctatgtca
     8641 accgtcaaca gagacaatct gtccgttgca ggacctgaca gatttagaaa tgttcaatct
     8701 gtctcttatg tattttaaat caatactcac taggagaagt ttcctaacct cctaatagta
     8761 atgctggtag taacattgtc aaaactgctt cctcttagga acaagcaaag cataacgagt
     8821 gtaaaaataa aatgtagact attgtaacat atcattgatt atttcaagta aaaacgataa
     8881 aagaagtaaa caaacactta aaaacaatgg aaattagtcc caactttaag aaatgtatac
     8941 tataatggaa attatcaatt atttatttgg aggaatatcc atgaaacaaa aaatcgcagt
     9001 tcttggtcct ggatcttggg gcactgcttt agcacaaaca ttggcagaaa acgggcatga
     9061 tgtccgtatt tggggaaatg tacctgaaca gattgatgaa atcaataagt atcatacaaa
     9121 tcagcatttt ttacctgatt tgacgatccc agaatcgatt atcggatata aagaattggc
     9181 agaagcagtg gataacgcag atgctatttt atttgttgtt ccaacaaaag cgattcgttc
     9241 agtcgcacag gaacttattg gtaaaatgaa tactaagcca atcattatac atgcaagtaa
     9301 aggactagaa caagatacgc ataaacgaat ctcagaagtt ttggctgaag aaattccaga
     9361 agaaaaaaga caggcaattg ttgtattatc tggtcctagc catgctgagg aagtggcagt
     9421 acatgatatc acaacaatta cagctgcaag tattgatcaa aaagcagcag cctatgtaca
     9481 aaaactgttc atgaatgatt atttcaggat ttatacaaat aatgatgtga ttggcgtaga
     9541 aactggtgca gcactgaaaa atatcattgc aattggtgcc ggcgctatcc atggattagg
     9601 attcggagat gatgcaaaag cagcaatcat gactcgagga ttggcagaaa tcagccgtct
     9661 tggtgtcgcg atgggtgcca atcctctgac atttatcggt ttgagtggtg tgggggactt
     9721 gatcgttact tgtacaagtg ttcattctcg caattggcgt gccggtaact tgttaggaca
     9781 gggacataag ctggaagaag ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac
     9841 gacaaaagca gcagtagagc tagctcaaca gctaggtgtt gagatgccaa ttactcaaac
     9901 aatctataat gttttgtata atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt
     9961 acgtgacgga aaaatggaga atgaattttc tcttcgttaa atagatgatc aatagttaat
    10021 c
//
LOCUS       DAFGFZ010000105         9959 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000105 DAFGFZ010000000
VERSION     DAFGFZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.107"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(1..153)
                     /locus_tag="LR726_002318"
     CDS             complement(1..153)
                     /locus_tag="LR726_002318"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL6217362.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(344..1222)
                     /locus_tag="LR726_002319"
     CDS             complement(344..1222)
                     /locus_tag="LR726_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL6217363.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1261..2379)
                     /locus_tag="LR726_002320"
     CDS             complement(1261..2379)
                     /locus_tag="LR726_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL6217364.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2376..4475)
                     /locus_tag="LR726_002321"
     CDS             complement(2376..4475)
                     /locus_tag="LR726_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL6217365.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4526..5347)
                     /locus_tag="LR726_002322"
     CDS             complement(4526..5347)
                     /locus_tag="LR726_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL6217366.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5344..6153)
                     /locus_tag="LR726_002323"
     CDS             complement(5344..6153)
                     /locus_tag="LR726_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217367.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6174..6668)
                     /locus_tag="LR726_002324"
     CDS             complement(6174..6668)
                     /locus_tag="LR726_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217368.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6680..7168)
                     /locus_tag="LR726_002325"
     CDS             complement(6680..7168)
                     /locus_tag="LR726_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6217369.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7245..9941)
                     /locus_tag="LR726_002326"
     CDS             complement(7245..9941)
                     /locus_tag="LR726_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL6217370.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3021 a         2252 c         1646 g         3040 t
ORIGIN      
        1 tcaccaaaaa aattcaaaca ttaagccaag gattatgaag tcaacatttc gaaaaatgaa
       61 catacattgg taagcaacta taactattac cacagacata aagtaacaca atatattact
      121 atttaagtgt acgccaccag tgaatatcct caaggtatac agcgcacaac aaaaagaaaa
      181 aaggaaacgc agaagcattt cctttcagtg atttaatgaa aacaacacga tttgacttag
      241 agccttttta tttgaattca ttttcgcttt atccaacttc atttgatcat ttgattactc
      301 ggttaaatac ttttgacatg agtatcaatg aaacattcat taattatggt tgcttatttc
      361 ttaccgcttt tctggccaat gctaatccgc cgattattcc ggcattatca cctaattcgg
      421 gcgtaataat gtagctatca attggtggta tttcaacata tccatttata agctcaacaa
      481 atttatcctg aacttttttc ttcatgtgtt cttgtttcat tacaccaccg ccaaaaataa
      541 ttctatctgg cgaaaacatc agtgttgtat tgtatgcaca ttgggcaagg taaaaggctt
      601 caatctgcca aaacgaatcg tcagccaata aattctggcc ttttacaccc aaccgttttt
      661 caatcgctgg tcctgctgcc atcccctcta aacaatcttg gtggaaggga cagtttcctg
      721 aaaaattgtc ctttggatgt cgcttgatca acatatgccc catttccgga tgactgaacc
      781 cttccacgaa agtcccattg aacaaggcac cacctccgat acctgttccc acagtgaagt
      841 atgcaacacc tgctaaccct ttgccagaac cagcaacata ttcaccatag caagccgcat
      901 tgacatctgt tgtccattca atcggaatat tgaaatattg ctttagcgtt cctacaaaat
      961 tgaaattttg ccaagcaagt tttggtgttg atgtgatatg accgaaactc gttgatttgc
     1021 attgaatatc aattggacca aagcagccga ccccgatgcc tgccaattgt tccttaaaag
     1081 gtttaaaaaa atcaatgacc tgcttcattg tttcaactgg agttgttgtg ggaaaactga
     1141 ctctttccaa aatcgttaat tcctcagtac cgactgcaca gacaaattta gtccctccag
     1201 cttcaattgc cccatatatc atcataggtt cctcccttcg aatatgtgaa aaacacttca
     1261 ttaacgcggc aagcgttctc gatcagtgaa atgacgttta gtatttcctg aagcttctgt
     1321 tgaataccag taagcgacac tagcaacatc gtcttgccgc tcaaataagc gaacatcatc
     1381 gttgccgatt tgttgtaaag tgaccgataa atcttcggta aaacagatgg ggtcttgaag
     1441 atgccaacga taaagaccat gtcgtggcat tgcgtcgtca ccaaaggcat gaacggcatt
     1501 caattttccc gtttcaaaac gatctcttgt ggcatctcta tttgattggt aaggataccc
     1561 taaaaaggct gttgaatagt ttttcgcatg tggacgtccg aactcatcgt actcatgaaa
     1621 ggcccacgcc ccgccaaaat aatcttctga acctgttgag gttacggtcg gatactcact
     1681 atccccatca atgtagaatt taaactcgcc ttcgccccac caataacgtt ctaggcaggt
     1741 aagcgcaagg taagtaccca cgtaatagcc ttgacccttt aatcgatcaa ttaacttata
     1801 atcctcctgt aatttggtaa tgcgttcccg atgccaattt gcgtgaaaat ataggctatt
     1861 ttcttccaat tcgtccacca aagcgtaatt tatcgtataa aaaaatgagg acatatcctt
     1921 cggatgtaaa ttctcgatcg taattttagc ggatgtttgg aaaggcattt cgaaatagga
     1981 attaaagccc cctgtaggat taaccactat cggcaacgag ttcacctcgc aacgctctcc
     2041 aaacccatta caaaagaaat cccctaaagg actttcaaca gcagggctat ccgacccatc
     2101 ccaataaatt cttaagatta aatcacgtaa aacaaaactg ccaaattctg ttcgatcacg
     2161 aatcgtcatc cacatatgac gaattattcc cgttccagta atatccgcaa tcacagtttc
     2221 ttcaccagaa gtcagcggaa ttgcgccacg acctttgcgg cttggtccta gctcgcttgg
     2281 caacatcgca gctttacctt tctcgcctgt tggattttcg ggagtgattg ctcgactctg
     2341 gattttttta aattgggtaa tattttttag tatactcatc tttttctcct ttgaatgtga
     2401 atcgtttttc ccttttctac gtgaagaatt tttttcgtat cacttatttt caatggcttt
     2461 tctgacaaat ttgaaatact caactcggat tgcgtacatt caatcgaaat gaaggcttga
     2521 ccgaacttca aattcttcca agaaaacgtt aaattatcaa caggatcaat ttgcaatact
     2581 ccttttttga atttcaatcc gaaatattga ctgatcatta ggctgacaaa aagacccgat
     2641 gcccagccgc atttgccgca gccaaagtct cgcacgactt ctccttgttg agcctctaag
     2701 cgatacggcc accaccacca cgaaccgtct aaatctgcca aacaaattag ttcttctaat
     2761 cgggttacga aagcttgagg gttgtgcagc tcgctcatta aaacagtgat aaagccagga
     2821 aaagtggcgc cggattctaa tccccaagtg atgcctttga tttcctcgct ataggttgga
     2881 ttgaaagaac tagcagaaaa ggccatggtg ttctgatata aatgatcttt attatctaaa
     2941 aattgataaa aaggcatcaa cgtagtgtct gattcttctc catcatgcat caataaatta
     3001 atttttccat ccacaatcac atcacttaaa aagattaacc cttgctcaag aatcggtttt
     3061 tgatagtgct tcactgaata ggttgttttc tcctcatcac cgattccctc caaaaattgc
     3121 tcaccaaaag gaccatcgat agtcatttgt ttacaaattg attcctttag tttacaggca
     3181 atgtttttgt atcgcttgct aagactcagt tgttctaggg cttcaaaaat ttctgcaagt
     3241 ccaatacagg ccttccaaag acaaagattg ctgccggtat gatactttcc aagtgcaaag
     3301 gcgtctgata accaaacaga tggaaaaagc attggttccc ctgaccgacg ttgagctaat
     3361 atgtcctcaa taattcttga agcattcagt agaacctgcg gacaccgctc aaagaatttt
     3421 gtatccccag tatgttcaaa ataaagacta gccagtaacg cactggataa cgaattactc
     3481 agagaatgat tgatacctcc tgtaaatttt gaaccaggaa acttaacgcc atattgatca
     3541 aaccatagaa ccgttttttg acataactcc ggttcaaaca tggtgaatgg caatgatgcg
     3601 taatacatat ccttattcca aattcgatta gtagcaggat agctgcccca attgctgccg
     3661 acgacctgtc cctgtcgatt cataccgaaa ctagaaaagc tttgataaag tgcacgacga
     3721 tagagatgga caactctatc gtttggcatt gacagctgcc caaatagctg agcaaaatag
     3781 ttcaatgttt ccttcagcca tttttcgata tcagattcct gaaaagtcat tacttcttca
     3841 aaagcatttg ggtccacaaa agcaatggcg aagcttgcca cttcctgttg tgcgatcgtg
     3901 tccttatttc ctttttgaga aatcaacaca ttttgctgat ccgaatactt ctcttgatac
     3961 gaaggcacat ctttcactga aacttgaagg ctttcgtgtg tttcattcac aatgtaaacc
     4021 tgttgaacaa gcatacttaa acgcttatta gccagtatgg gagcaaagga tactacaatg
     4081 accttaaacg tcgaataatg atgaatatat ctaggcaaac agtcttgaat cacatcgact
     4141 tcaatacgat gatccgattc tgacaaatgg tagacaatcc cgtttacttc aacctttaag
     4201 gataacgaat tggtttgaga gagctgttta ttgacccaaa cacctggttt ttcctcagtt
     4261 agtaaatttt ctcgataaaa ggtcaacgtt tgaatcgtcc cgcaaacatc aaggtttgct
     4321 aatgtctttt gattacttac atcgaaggtc aatcccttta catcaggaat attccgatag
     4381 tagctgtatt cgtttgaagt agtcacattt ccgtcgttta actcagcatt aaaaaatggg
     4441 tgtttgggtg aataaattac ttcattaata gtcatctttt ccctccttgc taaaaaaact
     4501 gcagcctagt agctgcagct tcctactatg cgattccaag taatgacaat aaaattccca
     4561 agacgaccat tccaaaaata attttgatga cgccgacttt tttatctagt aatttgaaac
     4621 agctcaatgt taccaataac ggtacaattc ctttaaaaat actatcgagg atttcttgga
     4681 cattcattag ttcttcgccc ttcattgaaa agctcaaaac ggtattgaat ttgaccatac
     4741 tgatcgtcat tgcacctacc atgatcagac ccaagatact agcagctttt gtcagaattt
     4801 caattaaccc gttcgcatac agcttttcaa tatatttaga gcctaatgaa tagcctaaaa
     4861 cgccgccata gtatcgcatc aagtaattag gtatattgaa cataactaaa aagataattg
     4921 gtgccaaaat atttccggca ttggctaagt tgatcgctag tcccgcggta acgacacgcc
     4981 aaactcccca gaacaatgaa tcgccaattc cagccaatgg tcccatcaaa ctcgttttga
     5041 tcgcgttgat actttccacg tcaaaaccag ctttttcaga attttccttc tccatcgatg
     5101 ccgcaatccc catgacaaag gacgcgcaag gaatggtcgt cgaaaaatac tgtgtatgac
     5161 gttccagcgc cgcagctcga tcttcttcac ttttgtagaa atgattaatg aagggcatca
     5221 tagaataggc gaaaccaccg ccgcccatcg tcaccggatt caccgaagca tacatagtca
     5281 tcgagcgcca aaacatcttc cgaaccaatt ttttctcatc tttagaaatt ccatcagcta
     5341 atttcattga aagaaatcct cctcttcttg gtcaagcgtt gcatttacag cagcatcgct
     5401 ttgaactgca tgaacagata tattttttag ttgtagatct ctttgggcta taacaattgc
     5461 aataatcgtt ccaactgcgg caattgcaat caatggtaac tcgagatacg ccataacaac
     5521 ggccccaaag aaaaaccaca cagcaatatt tttactccaa agcatccgta acagcatcgc
     5581 catcccaact gccggcagca gatttccagc gactgttaat ccgttcatga tgacctctgg
     5641 aatattatcc aagacgcttt tgacaacatc tgctccaaat aaaacggcaa agaatggaat
     5701 aagagcataa agcgcatagt ggacaaacca cataaagaag tgcagattga tgattccttt
     5761 ttgatcacca gattttgcaa atttgaaaaa tttagcagcc atcggtccac atacaaaaga
     5821 aaaaatcata ttcattaact gtaatcctaa tactgctagt ggaaagacaa tgggaataat
     5881 cgcatccacg tttcctccca ttttgattgc gaacgctgta gccagcccag cagccaaaga
     5941 aggctccgcc gtactagaga ttccgatatt gacgacaccc ataaagatgg cttctagtga
     6001 cgccccgaca atcaaccccg tctgcaaatc ccccatcaac gctcctgcta acggagcaac
     6061 aacaatcgga cgactcagca tggacatccc gaacatttca tgtccagcaa ctccaataaa
     6121 aacggccaaa gcgactacaa atgcatcata cataattttt ctccttcctt tttctaaaaa
     6181 tcactcaaaa tagagatagc tgaacgtttt tccatcgccg gtgtaggctg aaaaacggta
     6241 tctggataaa gttcaactaa ttcttttaag tcttgaattt ctgacgtcgt taacatcact
     6301 tcctttgata aggttttctt atcttccgat ggcggtgtca ttttcccagc attccccacg
     6361 ttcattaatt cgatttccgt tgggaaagct ttcgcaactt ttaccgcatc tttgacatta
     6421 gggactaaaa ccatcaagcg catgtccttc gcccgcggat cttttaacaa atgaatcgcg
     6481 tcatcaactg ttttaataat gactttgaca ttcgatggtg cagccatctt caatgtcatc
     6541 ttttgaatat cgttggtagc cgcctcatcg cttgctacta ctaacccggt taaatttaga
     6601 ctctttgtcc aagtcatcgc tacttgtcca tgaattaatc gttcatcaat tcgcactgct
     6661 ttaatcattt ttcttcctcc taaaaaaaat cttcctcttc atttactaca tgtacctcat
     6721 caagaatggt catctttaat tcctgttggc atttatttac cagttcctga atatcctcct
     6781 ttgttaattc cttggttgcc aatactattt cgagcagaat tggcagatta aagccagaca
     6841 aaatagttac agcaatctct ttctccaaaa aggttttggt aatcttctga ttgacacttc
     6901 cgccaaataa atcggtaaaa acaaaaatct gatcctgctc gttcacctgg tttacaaata
     6961 cattcaactc ctgttcaaaa tcacactcat ccacataagc attaatcacc gtaatctctt
     7021 gaaccttccc cgtcagtaat tgaatactgc taagaaagcc tgaagctaag tgaccgtgag
     7081 tcgctattag aattttcttc atatggttaa ttcctttctt tctgttcact tttatataag
     7141 caattcctgt gccaactttt aaaaacattg gtttaatcac gttaattcac attaaaaaaa
     7201 gtgtctaacc taaaaaaaac agattagaca cttttttaga cactttattt ttgaatatac
     7261 ggtcctaata attcatagag cagcgaaagc tcataagcat tgactttaaa attatatttc
     7321 atctccattg attgaaaaat acttctagaa acataataaa attcatcttc atctggtttc
     7381 aaatcataat tcggttcttc agatttttct gtccgagcca agaataaacg ttccatcatc
     7441 aatgccgtat gcatatagag atttagtttt acacggccat ttaaagtgaa atgatagtaa
     7501 ttttcatatt tagaaatgac tgtttccact tcactcatca cgatcgttgg gttcagaaag
     7561 ttcaaacggt ccgcaattcc ttccaacgaa aagaatttta ctagctcctg aatcaagcgg
     7621 ttaaattctg ccggcttcat ttcaggactc aaaatatgct ccaaattggc tgctccatca
     7681 ccgtctaaga tgtcataaac attaatatta ggaattgaaa aggaacgtgg aagatccgaa
     7741 gtcgtgatga ccaattttgt cttgtcaaaa taagtttcat cattttggtc gattaattca
     7801 cgtagttctc tgtagtcctt cgtgaaaact tcgatggatt catccgataa atgatgacga
     7861 aaaacatctt tcaatttgtc cgaaatcccc aaacccgaca tacaggaaat gataatgttg
     7921 ctactttgag aaaagccttc aaagtattga acattgattg aatacttgtc tgctgcatcc
     7981 tccgcaatct gtttaaatgg cgcattcatc gtcatcttca agccaatatc aagtgcaatg
     8041 gaagtcgtca aattattcag aagaagtaac tcccctttta aatcgttctt gatctgtgtg
     8101 taaatttgat ttaaagagcc catatctacg atcagcacta gactatctgt attcacttgc
     8161 cgtttcacga aattccttgt cgattcgaca atctctgaca cattactctc tactggcata
     8221 tccaacgctt caaaaacata attgccgcat aattgattca ctactgcttg aatgctgcta
     8281 gccgtactat ctccgtgagc aagcaacagg cccttcagct cgatggattc atctacataa
     8341 tcagataaag tcacgacaat aagcagtctt agaaagttct cgtccttttc tgagagcggt
     8401 ggtaaatgct ccaagaactg attacataag taagtcgtcc gaggtagatt tgcagctaat
     8461 gtacgattta tcccggttaa gtctgtctta tagcttgggg aaatgacata ggatctgcta
     8521 ataatttctg caacaggtcc actcgtcgtc aatccgtacg tttttcctaa aacggatacc
     8581 cagacctttc taaacatctg aatcatcaat tttctactca ttgataaaaa agaatcctta
     8641 tcaatgtgat gcaccaaatg ctgtacttca ttctttatcg aagaaaaatt ttcattgaaa
     8701 ttaccttcat ttatcagctg agtaattttt tccaaaatcg aatcataatc aaacggtttg
     8761 attcgatccg tttttatctt ttgatttttc tttatcaata aactagcaat cggcaagctt
     8821 tctacttcgg attccagcat atttaactga atcaagatct catttttccc aatcgttaat
     8881 ggttcttctt gtgttcgact atacacatcc gcacaactga ttttaatgat attcttcagc
     8941 ttaccaatat tcccactaaa gggatgagat aacattaaag acaaggcttc tcttgaaaca
     9001 ataatatctt tttgtaaaac atccgcttca ttttgaaaaa ataattgaat cagctctaat
     9061 cgttctcgaa tggggcgatc ttcaaaagtt ggcaacataa cactaatttg aatacgccga
     9121 tcaaaggtgt ctaataaata attttcacct ttttccgtcg tggcgaaaat aaaacgaacc
     9181 ttagcagaat gccaatttac attttcaccg actggacgat agtttccagt atcaataaat
     9241 agaaagagct tttcttgatt ttctttcgat aggcgatgga cctcatcgag aaaaagatag
     9301 ccgttgttgg ccgttgccaa taaaccatcg gtatcctttt cagcccccgt aaaggccccc
     9361 ttcttgtatc caaacagtgt tgccgaaagc agttcgggat tgtccgcata atcggcacag
     9421 tttaagatag caaatggtgc atctttttca ataatttgct catggatagc gtattcaaaa
     9481 atttttgtgg ctaaaaaact ttttccaaca cccgtggctc catttattaa tacactgagt
     9541 ccgttaggcg gatatttgac agcggctata catttttgaa tgacctctct cagacttccg
     9601 tcatgaccaa ctacagaatg aaaggcttcg tttatttgct ccgtattatc tacaaccgca
     9661 tattcatcta aaggcttcca caaaacaggc tttcccggta atttctcgac ctttttatct
     9721 tccactaatc tcgtcaaata atggctggca ttctgacgag agatacctag attcttcgaa
     9781 attttctcgg cagtgacaga agacatattc cctgttagtc gtatcagctc atcaaaaact
     9841 cgatctttag cgctcttttt cattttttcc tccagtttaa tgtgtctaaa agcataataa
     9901 actatcggct gcaatatgtc tattctatat ttgtattcca atttttactt tgaaacaca
//
LOCUS       DAFGFZ010000106         9949 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000106 DAFGFZ010000000
VERSION     DAFGFZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.108"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(172..2292)
                     /locus_tag="LR726_002327"
     CDS             complement(172..2292)
                     /locus_tag="LR726_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL6217371.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2522..3700
                     /locus_tag="LR726_002328"
     CDS             2522..3700
                     /locus_tag="LR726_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL6217372.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3716..4474
                     /gene="aroD"
                     /locus_tag="LR726_002329"
     CDS             3716..4474
                     /gene="aroD"
                     /locus_tag="LR726_002329"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL6217373.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4497..4982
                     /gene="ybaK"
                     /locus_tag="LR726_002330"
     CDS             4497..4982
                     /gene="ybaK"
                     /locus_tag="LR726_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL6217374.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5053..6306)
                     /locus_tag="LR726_002331"
     CDS             complement(5053..6306)
                     /locus_tag="LR726_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL6217375.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6423..7772
                     /gene="gdhA"
                     /locus_tag="LR726_002332"
     CDS             6423..7772
                     /gene="gdhA"
                     /locus_tag="LR726_002332"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL6217376.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7884..9230
                     /locus_tag="LR726_002333"
     CDS             7884..9230
                     /locus_tag="LR726_002333"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL6217377.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9538..9924)
                     /locus_tag="LR726_002334"
     CDS             complement(9538..9924)
                     /locus_tag="LR726_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL6217378.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3179 a         1804 c         2036 g         2930 t
ORIGIN      
        1 attcctttag tgaaatcacg aattaaaacc agaaaatatt ttgtaaaaag caagaggttg
       61 tgaagaagct gccctattct attaccgtag atgcaagctt ttgaacactg tttattcgaa
      121 aagaggagca gcgacaataa ttttgtcgct gctcctttta aatttatttt atcattcgga
      181 agaacttgtg gtcgttccgt gaagttcttg gtacgtttgc gttttataaa gatcaacagt
      241 tgattgtccg ttgtgttcac taaagacact agttgacttg tcaccaagag atttttcagc
      301 ttcttttaat ttctctaagc cattcttgta gtcataatca ctggtatcaa ttgggtctaa
      361 tccactattc gtatagaacc gaagaagatc accattattg atttggtcag aaacagctaa
      421 ctgtttatca actttttctt tcagtgaatc aatttcattc tgtacttcct cagtaggttc
      481 agtaatagct tcaccagttt cgttactata gatcgtacca ccgtagtaag tatattttgg
      541 tgtaacaaaa tcaccatcac ggaaagaaac aagctgttca tgatctttcg agaatagatc
      601 ttgtcctaac tgaatataat tttttgtatc tacacctagt aagtgaagta atgtaggcaa
      661 ggcatcaact tgtccgccgt aagtgtgatt gattccgccc tgtgtttgtc cagggatatg
      721 gatcatataa gggacacgct gcatttgtgc attgtcgtag tttgtccaag tagaacttgt
      781 ttttccaact aattcagcta gatttttatt tcttgaattc gagacaccgt aatggtcacc
      841 gtaaaggacg atgactgaat tatcatacaa gccgctttct ttcaaataat tgaagaattc
      901 ttgtacggcg gtatctaaat aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt
      961 tgtattggct aacgggaatc ctgcttcgtc attggtgaat tttgaataag gataatggtt
     1021 agatacagca atgaattttg aataaaatgg ttgttgcaga cgttccaagt attgtacgga
     1081 ttgttggaaa aatggtttat catgcaaccc gtattggaac gaattgtcat cattaacatc
     1141 ataataagaa gaatcaaaga aataatcata tcctaaattc ttgtatgtct cgtttcgatt
     1201 ccagaatgta ccagcgttcc catggaatac cgcactaatg taaccttgtg tttgacttaa
     1261 aatatcaggt gcagcttgga aagtattttt tccgcctaat tgtgtgaaca aggagccttg
     1321 attcaagcca aacaatgaat tttccatcag cgtttccgca tcgcttgttt ttcctgcttt
     1381 tacttgatgg aaaaagttgt caaaactgaa cgtgctgttg ctgtggtaaa gactattgat
     1441 gaaaggtgta acttcatgct ggagaccatt ctcatctgtc aatttataat cgatcaggaa
     1501 ttgctggaag ctttctaaat ggatataaat cacatttttt cctctagcga gcccgaacaa
     1561 atcgtcattt ggtgctgcat aatgttcttt tacataattt tctacttctt tcatatcatt
     1621 gggacttgct tctgcccgca cttggtttgt ctggtaagtt tgaacaccgt cataaaccat
     1681 gaaagcattc aagccaagat atttgaccaa ataatcacga gagaatgttc gagtcagcaa
     1741 ttctggacga tcagcttccg ccataaaaag atttcctgag aaaatcataa ctgctaatgt
     1801 agagatcgca aaagccattc ttgctcggat cgggcgaggg tccaacttga ttttttttgc
     1861 aatcagcaga acagtcaaga taataaaatc gatccaatag atcacatcat atgggcgaaa
     1921 cagtcgaatg gcactttctc ccagaccact cgctactttc ccagcaccta aaatcgtgtt
     1981 gatcgtaatg aagtccgtga attctcgata ataagtgaca ttcgaaaaaa caagcaatgt
     2041 caaaagaaaa taaatgacca tcatcgtgat ataagcaact ctagggcgtc tgatataaag
     2101 agcaattcca agtaataaca atgtagttgc aatagggttg ataaacaaga taaagtattg
     2161 aatgccactt tcgattccga gatggaaatc aactaaatag gcaaaaatcg ttttcaacca
     2221 aagtaaaacg actagtaaag aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt
     2281 tatacatttc aagataatct tcctttctgc acttcttaat cacagaagtg tcctgaaacc
     2341 atgttacaca gctcacttat tctcatttat tttactagta tgacaatata gtgtcaattg
     2401 aaaccagaag tttatgctgt ttcattaagg ttttctttaa gcttcatgaa aaaatgagca
     2461 gtaagacaag tcctatgttt tttgttatac ttgggaagag aaagctaaag caggtgaagg
     2521 aatgaaagta aaagtaaaaa aaagagcaag caatcgattg aaaaaaggat atccactgat
     2581 ccaaaaagaa gaccttgctc atccccaaaa agaaaccacg caatgggtgg aattagttga
     2641 ttcgcaaaac caatttatcg caaaaggata tctaggaaag cagaataagg gaatcggttg
     2701 ggtattgagc cagcagaaca tcccatttga tcaatctttt tttgaagaat tttttgtgaa
     2761 gtcaaaagaa aaaagaactt tctttttttc agatgattct actaccgctt ttcgtatatt
     2821 caatggagaa ggagacggga tcggcggtct gattgttgat tattatgcag gttatgctgt
     2881 tttttcttgg tataacgaaa cgttatatgc acatcgtgaa ttattgattc aagcttttcg
     2941 tgaaatctac ccggaaatca taggaggcta tgagaaaatt cgatttgaat cagtaaatct
     3001 gcctgagagc cagttcattt acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa
     3061 cggagtaaaa tttgccgttt atttaaatga aggattaatg actgggatct ttttagacca
     3121 aaaagaagta cggggatcat tagcagaagg tttcgcttct gggaaaacag ttttgaacat
     3181 gtttagttat accggcgcct tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac
     3241 aagtgtcgat ctagcaaaac gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg
     3301 tttggatgtc gatcagcaaa aaatcattgt tatggacgta ttcgagtatt tcaaatacgc
     3361 caaaagaaaa caatttttat atgacatgat tgtattggat ccaccaagtt ttgccagaaa
     3421 taagaaaaaa gtctttcgcg tagcaaaaga ttatggggaa ctggtcaagg atagtctgga
     3481 tattttagca gaagacggga tacttattgc gtctacaaat gctgcgaatg tctcgataga
     3541 acaattccaa acaatgattg aagaggaatt ccatgccaaa aatgtttctt ttgaacaact
     3601 tgctttatat cgtctgccaa aagatttcca gacgacggat acctttttag aaggcaatta
     3661 tctgaaagtg tttgtttatc aaattaaaaa taaccattag aaggagcgat gcagaatggt
     3721 tcgggtaaag ggattgaata taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg
     3781 ggccgtccga gaggaagtat tacgtgaagc agttcttgca cgtgaagcag gggccgattt
     3841 agtcgaatgg cgagtcgatc attatcgtga agttttttct tttgatgaag tagcagaaac
     3901 attggttctt atccatgaga tgcttgatgg actgccgtta ttatttacgt ttcgtacctt
     3961 agctgaaggc ggagaacaag acatatcgat ccgtcagtat cgcgaccttt atgaacatgt
     4021 gatacacaca ggattagtag atatggtcga tatcgagttg ttcaaagtag aaagtctagg
     4081 aaaaagactg atagaggaaa tcaagtcttt aaacattcct ttaattatca gtagtcatga
     4141 ttttaaagaa acaccagcag accctgtctt gctttaccga ttgaatgtta tggaacattt
     4201 tggagccgat atcggaaaac tggcagtaac tcccaataat gaacgagatg ttttgcggtt
     4261 gatggagttg actagacggg caaatgcttt tttctccatg cctattatta cgatgtccat
     4321 gggaaatctt ggtaaaattt ctcgtctggt tggcggaacg acgggttccg ttatgacatt
     4381 tggttcctta agcagcgatt ctacttctgc tccaggacaa ttaccggtca aagcattgaa
     4441 gcaaattatt caattattaa atgaaccgtt gtagaacaag tttgaggtga tattttttgg
     4501 caaagaaaaa aatagtaaaa acgaatgcaa tccgtatggt cgaacaaaaa aagattccct
     4561 atacagagca cgaatatgag tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac
     4621 agcttccaga aagccaatct cgtatattta aaacactggt tgctgtgggc aatgtgacgg
     4681 ggcctcttgt tgctgtgata ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag
     4741 tttcagggaa taaaaaagta gaaatgctcc atttaaaaga tttggaagca acgaccggat
     4801 acatacgtgg tggctgctcg cctataggta tgaaaaaact atttcctact tatttagatc
     4861 aaattgcaga aagctatgaa caaatcattg tctcggctgg cagaagaggg ctacagatgg
     4921 agttagcacc tcaagatatc tgtgcattaa cgagcggaca atttgcagat ataaaacaat
     4981 aaatcatacg tataaaagaa agaaggaggc cgggatgttg tttgtccagt ctccttcttt
     5041 cttttttata cattatgctc tttgcccggc attcattcct gctacatgcc ctgtagcgaa
     5101 agccgctgtg atattaaagc cgccagtata accattgaca tccagtaatt ctcccgcaaa
     5161 atacagacca tcaatgattt tactctccaa tgttttagga taaatttcct ttagagaaac
     5221 ccctccgcca gttacaaatg atttctcaat aggaaaagtt ttgttgattg atagcttaaa
     5281 ctctttgcat agttgaacaa aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag
     5341 atgatccatt tcaagccgct caagaaagaa aactaacaat cgctcaggca atataccatg
     5401 ccaagcattg accaagttct ttttggttgc tttacttttt tctgtcaata tatgaataag
     5461 ctcttgtttg gtttgtgtag ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt
     5521 ctctaattct ttattgacaa aacttgaaca acgcagtgca gccggaccag aaataccgaa
     5581 atgggtgaaa agtaaatcca tcgtatgttc agtcaataca cgaccttttt tatttaaaac
     5641 gcgtaaagtg atgtcttgta aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc
     5701 atccgatatc aagggggact ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt
     5761 cacgatcttg tacccatctc cagtagaacc agtagatgga taagtccgtc caccagttgt
     5821 taagataatg gcaggagcat ggaactcttc gaattccgtg cgtataccgt aaattttatg
     5881 ttctttgtgg atcaatttct ctactcgagt agagaataga agcgtcacat ctagttcttt
     5941 cagacgattg agcaatgctt cgataatcgt ttttgattta tttgttacag gaaacatccg
     6001 tccatgatcc tcttctttta aatgtacgcc ttgtgattca aagaagttca tgatatcgaa
     6061 attattccat tgcgcaaacg tactatataa aaactttcca tttccaggaa tatgtgcaat
     6121 caaatcatca acaggacgat tgttcgtaac attgcaccga ccaccacctg tcatcagtag
     6181 tttttttcct gcttttttgt ttttttcgat caagagtacg cgtgcgcctt gttcagctgc
     6241 actgatcgct gccatcatcc cgcttgttcc agcgccgact acgatcacat catacagttt
     6301 ttccattccc tcacctcaag gaaagtgtag catatctgaa gaaagaaaac agaaaaaaat
     6361 gttttgagcc aaaagagaaa atgagctaaa atgagggaag ataagcaaag gagcgtggaa
     6421 cgatgacaaa cgcaacagaa tatgttcaag caattcaaga aaagctacat caaaaggatc
     6481 aagggcaaga agaattttta caagcaatcg atgaattcat gcccacggtt atgtcttttt
     6541 tggacactca tcccgaatat atcgaaaaga atatcttaga acttttaacg gaaccagaaa
     6601 gagttattca atttcgagtg ccttggcaag atgatgaagg aaactggcga gtaaatagag
     6661 ggtaccgtat ccaatacaac tcagctatcg gcccttataa aggtggattg cgcttccatc
     6721 ctagtgtcaa tctaagtatc ttgaaatttt tggcttttga acagattttc aagaatagtt
     6781 taaccggtct gccaatcggt ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat
     6841 ctgataatga gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg
     6901 gcccatcgat tgatgttcct gcgggagata tcggtgtagg agctagagaa atcggctatt
     6961 tgttcggcga atataaacga ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt
     7021 tggatttttg gggaagtaag atacgtacgg aagctactgg ttatggatta gtgtattatg
     7081 tcaaacatct attgaacgaa gaaaaagatt catttaatca aaaaacagtt tttgtttccg
     7141 gaagcgggaa tgtagccatt tatgcaatcg aaaaagtgca agagctaggc gggaaagttg
     7201 ttacatgttc agattctagc gggtacatct atgatccaga aggaatagat gtgaaactat
     7261 tgaaagaagt caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt
     7321 ctgctattta tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt
     7381 tgccatgtgc tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg
     7441 ggatcaaaat cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc
     7501 ttcatgaagc aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag
     7561 tatctgcttt agaaatgagt cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg
     7621 atgagcagct ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg
     7681 agtacggcga cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg
     7741 ccaaagcaat ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc
     7801 agcattttgt ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag
     7861 taaacctata aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac
     7921 cgtttgtcaa cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg
     7981 aattacggga aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga
     8041 actatgacaa agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt
     8101 ctgaagtatt agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg
     8161 acttcttgaa tcacacattc ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa
     8221 tcttctttgc cggaaattca atcagctcaa catatattgc tgacttgatc gaagtaatcg
     8281 gtgatcgcga cttttcagta aacgttattt caaaatctgg aacaactact gaaccagcga
     8341 ttgcgttccg tgtattcaaa gagcttcttg ttaagaaata tggacaagaa gaagcaaaca
     8401 aacgtatcta cgctacaaca gataaagcaa aaggtgcagt taaagtagaa gcagatgcag
     8461 aaggttggga aactttcgtg attcctgatg acatcggcgg acgtttctct gtattaactg
     8521 ctgttggttt gttaccaatc gctgttagtg gtgcagatat cgatgcattg atgcaaggtg
     8581 ccgctgatgc aagcaaagca tactcaagcg acaaactaga agaaaacgaa gcttaccaat
     8641 acgcagcaat gcgcaacatt ctttaccgta aaggcaaagt aactgagttg ttgatcaact
     8701 atgaaccagg aatgcaatac ttctcagaat ggtggaaaca attgtatggt gaatctgaag
     8761 gaaaagacca aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat ttgcactctt
     8821 taggacaatt catccaagaa ggacgtcgca atatttttga aacagttgtg aaggttgaaa
     8881 aacctcgtaa atcaatcaca attcctgaac aagcagaaga tttagacggt ctaggctact
     8941 tgcaaggaaa agaagtggac ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc
     9001 atacagacgg tgacgtgcct aacttgctag tcaaaattcc agaaatggat gcttatacat
     9061 tgggttatac aatgtacttc tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa
     9121 tcaacccatt cgaccaacca ggtgtagaag cttacaagaa aaacatgttc gcacttctag
     9181 gcaaaccagg ctttgaagat cttgctaaag aattgaacga acgcctgtaa gataagggtt
     9241 tttcgctaga tttaaataat cagtgataga aacatgttcg ctttaaaatg aaaactggta
     9301 cccatttagg tactcaaaca tactgcatcg tttattcaga ataaggggct gtgacaagac
     9361 gtttgtcaca gtcccttgtt tgtatagaaa tcgattgatt gccttgctct ttagtttgtt
     9421 ttaggacttt aagaacagtg aggctgactt cgaaggttaa ttctgttttt ttgttttcta
     9481 gaatcagtaa gcaaaaaagc actcacttcg agtgcttttt tgcgatatat tcttagctta
     9541 agataggcct aatttttctt ttacagatga gggtacctta tcgctggtaa ttactttttt
     9601 ctctatgatc cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga
     9661 accattggca gagtcaccag aagtaatgac atcgaaatct aactgtcttt tttcaccgtt
     9721 ttcagcataa gcatctacgt tgtatttaaa acctcttcca attacttccc cattatctgc
     9781 tttgatatca gtttctgcag gcattggagc tttgactacc gcataataat ctttacctac
     9841 ataagtagac tgatagtagt cccaaccttt ccatcctata aaagcaatca atatgataac
     9901 gatcaaccct aaaatttttt tcatgaaaga tctccttttt tatttttat
//
LOCUS       DAFGFZ010000107         9616 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000107 DAFGFZ010000000
VERSION     DAFGFZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.109"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            152..358
                     /locus_tag="LR726_002335"
     CDS             152..358
                     /locus_tag="LR726_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL6217379.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(560..1561)
                     /locus_tag="LR726_002336"
     CDS             complement(560..1561)
                     /locus_tag="LR726_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL6217380.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1566..3479)
                     /locus_tag="LR726_002337"
     CDS             complement(1566..3479)
                     /locus_tag="LR726_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL6217381.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3647..4153)
                     /locus_tag="LR726_002338"
     CDS             complement(3647..4153)
                     /locus_tag="LR726_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL6217382.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4313..4753
                     /locus_tag="LR726_002339"
     CDS             4313..4753
                     /locus_tag="LR726_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL6217383.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4779..5936)
                     /locus_tag="LR726_002340"
     CDS             complement(4779..5936)
                     /locus_tag="LR726_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217384.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5939..6295)
                     /locus_tag="LR726_002341"
     CDS             complement(5939..6295)
                     /locus_tag="LR726_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217385.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6593..7567)
                     /gene="sgrA"
                     /locus_tag="LR726_002342"
     CDS             complement(6593..7567)
                     /gene="sgrA"
                     /locus_tag="LR726_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL6217386.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7763..8602)
                     /locus_tag="LR726_002343"
     CDS             complement(7763..8602)
                     /locus_tag="LR726_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL6217387.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8787..>9616
                     /locus_tag="LR726_002344"
     CDS             8787..>9616
                     /locus_tag="LR726_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL6217388.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     V"
BASE COUNT         2884 a         1943 c         1445 g         3344 t
ORIGIN      
        1 tatctgattg ctgaatcatt aattgcttat cattagccat ctttgtttca agttcgctag
       61 atgatcattt tattcacatc ctaacaaact tgtcacattt atagattctt aatctattta
      121 tcatacaatt ataaaaaagg aggtgctgct tatgaaagct atccatattt ctttgctttt
      181 tttcgctagt gcattgattt tatttgctat tcatctttgt tcccagcctt atatcaataa
      241 caacggaata ttagtggaac cagcattctt ttgcttacca ctcggctttt tgtcccttct
      301 tgcctccgca ggcttcggat ttgttgcttt ttttaaacga tcaaaacaac aaatctgact
      361 tataaaattt acgtaaaaga gatcgtgaca ataatcgtgt gatattccca aatttcgaat
      421 aaaaggtgcc ccttgccatc tctaactcct cagtcacaag ttacaatatt cataaaccat
      481 gaaaagcttg tttattgaaa ttgctccttg tgacttggag ctaagtgaca gattacaacc
      541 tcttttgcat cataagactt tactccttga aaaacattac taacttttga tatgtttctt
      601 gcgcttgttt attgctgaaa tcaaactgat attcatggtt caaaacagca tttttctccg
      661 cataatctac ttttgtgact ttcacccccg ctttctcaaa tacagaagtt acttcttcca
      721 tttgtttcgt aaacgtattt gtatttccat ccgttaagaa tgtgttttgc gtccaacgat
      781 ccgcatgtct gaccaaggct aaacttttga ccttctctga ttgttcaaag tccttattat
      841 caaagtaagc ccatccacat ttttcataaa gaaagctaga gattttatca cttacttgag
      901 taagtgcaga aggatccaac agaccagaca aagaaataaa cttcgtaatc tgcttgttct
      961 ccaaagtcgg atcaagatga tcagcttttt taatttcttc attttgaagt gctaacaaat
     1021 attctccact aatttgtgca cctgccgaat ttccaccaat cgaaatattc gaccaatctg
     1081 cttgatcttt tatctctttt ttagctgctt gaatggcttc gttcaattgg ataactggtg
     1141 taggatgttt tgcttccggt gatcgttcat aatctatatt tacgatgatt tgctgcgtgt
     1201 ctgaacatag atattccaaa taatcacgac aatcattttt atcccctcct acatatgcgc
     1261 ctccatgcat ccagaaaaga atcggtaatt ttttcttttc cactttcggt gtgtagatat
     1321 ccatattatt atttttgtac tttgaaggat aggatatttc tttttttata tcgatttgtt
     1381 cgattttttg ctggaaatca tctggtcggg catatacttg ttctttttca cttgtgtcaa
     1441 attgttttct aacaagcaag ctaatcggct ttggtgtgat ttgattaatc aaaaatacag
     1501 tgactaaaat aactacgatg ataactggta ctgcaatcac tattcgcttg aaccatttca
     1561 ttcatttacc ctctctttct tcgccaaatc catgtgataa tcgtgctaac agataaaaat
     1621 ccacctgaat aaatgagaag cgaatgtttc ttttctccag ttttcggcaa cattcttttt
     1681 tttgcatctg tctttttttg tactttcact gtcatctgaa tcggttgcgt atcatcactt
     1741 gattgatcct ctcgaggttc attctttccc ttattgtctg cattctctgt cggatcttgc
     1801 ttttttggtg gagctactgg gatttttttg taatagaaaa taatagttgg tatcgtctca
     1861 ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt cgtatccttc gaatgtcttt
     1921 tgctctgtca tataattttc tccataagct ccgtaaagct ggatttcgtc ctggtctttg
     1981 agtagacttt tattgttttc atccacatac tttacgttta tctgatcatg gaacactact
     2041 ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt caacttcctg tgcctttcca
     2101 tgccctgctc cttctactaa taaaagatct ttataccctc ctgataactc atataatttt
     2161 tgcgcgttag acacaggtac tacatcatct gctgttccat ggataatcaa tttaggcaat
     2221 gaatctgaca ttccaatcgt aggtagagca gcttttgtat ccaaatgcat ctcctgatca
     2281 aaatagtatt gtttcaaaag gcccataaat aaatcttcat gctcttgatc cccaatgatg
     2341 tctaactgat tttttatggc atccggtagt gcgggggcta ctgtctggct gtacgtaaaa
     2401 cgtagttgtt catcaattga tgcaaatccg caattttcaa taattccttt gacattgaca
     2461 ggaatatctt tcgctaatac agacatcatc gtagctgctc ccatcgaacc accataaagt
     2521 aaaatctttt gagatggccg ctctcgaact tcttgattta tccagtaaag gacgtcatct
     2581 gactcatatt gtccataggt cacataatcg cctccgctat taccagtcgc tctggaatca
     2641 acaaataata aattataccc ggccttataa aaatcattgt attcttctgt aacgatcccg
     2701 ttattccaat tccctcgaaa tccgccatgg atcatgactg ttttatctga accttgatcg
     2761 acataatatg catatacatc catcattttg ccatcttgtt tgtttagtac agtttgatga
     2821 agttgttttg tatttggata gaagcctgtg ttttctgtag gcgttgtccc atcatctgta
     2881 cctccaagca tcgacatcaa cacactgttt tttgcaaaag cgatatcgta caatagataa
     2941 gtagcaatca gatgtttgat aatctcttta tctacaccat aatcattaac agcaagctca
     3001 gtgactaata actctattaa ttttgacttg atgtcttctg ttggtccatt aagtatttct
     3061 gtcatctgtt taccgaagta attcaaatat tctttaccat tgaaattttc acctttaccg
     3121 atatcatcat tttctgtccc attctctttg tcacttactt tagcagaaga ggattcgtcg
     3181 tttgacattt ttgtagctgt tgcttcttct gtttgttggt tatctgtggt agcggtcgtt
     3241 tcttgatctg tgctagtaac agatgtctca accgttgttt cagtatttgt ttctgtttgt
     3301 gaagtactcg atttcgtttc atttccagca tcagtagttt ctgtggtttc tacctcccga
     3361 gattccgatt cggaagaaat gctctctcca tatactgtcc attgcgggac gcttgtactt
     3421 acagcaaata agatgaccaa cgctcccttt aatacttgtt ttgttctcaa gctattcact
     3481 cctttttatg tttctaggag aattatatgt aaacgcaaac attttttata caaaaaataa
     3541 atggtttatc ccataaaaat agaacaggat tattttctca ttttacgtaa taaagcagaa
     3601 tggataaacc agtatccatt ctgctttttt taaacgaaga aacaacttat tgctccaaaa
     3661 atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc gattgtttcc tttgctttca
     3721 taaaaggatt atcataagtt gtcgttgctc ctctacacat cataagctgg tatcctaatc
     3781 catgagcaaa cttcacagtc gtatctatac aatactctgc ttgtgcccca cataattcga
     3841 tcgaatgaca gtccaattca gttaataaaa cactcaaatt agtcttataa aatgaattcg
     3901 catgggtttt tctcacaaaa taactgtgct caggaataga taattccgac aatagctccc
     3961 atggttttgt ttgtggcact aattgagcat cttcgtgttg aacgaaaata actggtttcc
     4021 caatcgtttc gtatgttttg attcttttat tcactaatgc tgtcactgtt tctaattcgt
     4081 gtatcggcga agaatcaaaa catacccctt tttgtaaatc gatcactact attgcgtcgg
     4141 ctgtgtttga catacttgtg ccttctttct tttttcttga tttaatagta tttcttatct
     4201 agctagttcc attatacgtg acaacatttt cttattttac acctctaaat ggcacttttc
     4261 gactatttct tcatgttatt atttcataaa tacttagcta ttggaggaat gtatgatgat
     4321 taaattgtat acaggttctg gctgctcgtc ttcaagacgc gcaaaacgtt ggcttatcgt
     4381 ccatgatata ccgtttatcg aacagaactt aattcgtcaa ccactaacca aaaatgaatt
     4441 tatccactta ctaaaattga caaacaacgg cgtgaccgaa atgctctcta ctcattctat
     4501 tagttataaa aatcttgttc acagaataga tgaactcagt ttgagtgaac tatatattga
     4561 gattcgtaca tccccttctt tattgaaaaa accgattatc tacgaccaat ctgcaaaact
     4621 tttgactgga tttagcgaag ataaaatcag gatgttcctt tctaaaacta aaagaaagca
     4681 agagctcaac gaactcttgc tttctggtca tcttgtcgat tttaaagaaa ataatctttt
     4741 tgcaatgtct taattttggc ataaacaatc tgcttttctt aattaacgaa atccaatttt
     4801 tccaatatgg atctattttt ctcattcctg atttttatga tttcagcttt cagacgcgcc
     4861 caatcatttt ttgtaggttg tccattccag taaaatatct gtgtattcgt ttgatcatat
     4921 gcctttttgt cgataggata atcgctaatg acaatatcag gtaactgatc agcggatgca
     4981 ctatatttta ttgggcgttc agtcatagat aatatccttt tctttgccca taattcctgt
     5041 gttttaccat actttgattg aatatagaat atgacaggtt gttcgtgttt tataaaaata
     5101 tccctgacta acatacagta ttgaaaaatc aaccgtctgt tcttttcata ataagtatta
     5161 agctctggaa ttctgcgagc taccccacta aaaaactgtt gcatgattgg atacatcact
     5221 ggaaatatat gctgaaaatc tctgtcttct gctgattttc caaaagcatc caccttcttt
     5281 tttgtcccaa aaacccttga atacaaatgg atcattgcca aattgacata tagataattt
     5341 ttttctgaag ttgtaaaaca aatcttgaag tttttttcaa aattttctac aatagacgat
     5401 tcaatccagc cccaactttc ttgtacttta tctggagtat ccaacaaatc ttcttccaaa
     5461 taggtgttca ttacaccaaa cataaaataa aggtaagctt gctctgttgg agtgatgtga
     5521 ttcatcgacc agaagtttgc ttcaaaaaaa ggctctagta tgtaatgcgt aaactcttct
     5581 tttgttatca atggatgcac aaatttgttc atctcttctg ggacatacgt aatcgagtgg
     5641 ccttgtttca ttcgttgcaa cgaaattctt agtaggctga tccatttctt tctatctata
     5701 ctacgcgtat atggcgcatg tttcgcaaat ttttctaata gttgacgttt attttttata
     5761 cttgataata tatcagaatg attttcgtcg aatgactgcc aatacatcaa gtgaaaaaag
     5821 tatcggatac ggtattcttc tccttctatc ggattacctt ttttcaagct gcaagataaa
     5881 tgatagtttt tcaaaatagt attaagttct cttaattttc tttgaatcgt acgcaaactt
     5941 atataatttt ttgactaaga ttttctaagg attgataagt acctttgtaa agtgaatcca
     6001 gtaaaatata accaatcgtt ccctctaaaa gtatagaata aactttactc agtaaaaact
     6061 gagaagaaaa ctcgactttt atcatcttgt tttctgaaag aaaagataat ttatattctg
     6121 gatacctttc gcaaaacgat tctgcttgtt gaatcaccga attcatctta gccagggtgt
     6181 agtttagatc taaagcgata tccttcacca catataattt ctcgtctctt tcataacaat
     6241 agttcaaaaa ttgatatact tgcctttcat tgatatcaat aaattcttca aacaaagtac
     6301 atactccttt ttatctatta tttagactta ttccgacagc tacatgaata acgtacaaat
     6361 agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt caattattct
     6421 tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca
     6481 aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg aatgaaatat
     6541 tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt ctttatttca
     6601 ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt aacacaccca
     6661 tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg ctttttctat
     6721 tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa tcaggctgtt
     6781 ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt ggtatgatag
     6841 gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg
     6901 tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc tccgtactgc
     6961 tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     7021 tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc tgtactgctt tcaccgctcg
     7081 tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt ccaacaactg
     7141 tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa aaaacaatca
     7201 aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa atcattattg
     7261 tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca ataacttttt
     7321 ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca ctagcattca
     7381 cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc
     7441 gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca gaagtactga
     7501 ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg gtcgctgttt
     7561 tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa ttatataaaa
     7621 tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc
     7681 gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc aagaccgata
     7741 tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc atccgctttg
     7801 ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat gtagttgata
     7861 tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat tggatcagag
     7921 tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata gctccttgtg
     7981 tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta ctgtagataa
     8041 gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat gtcatgttag
     8101 gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct attaacggac
     8161 caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct gatgccccca
     8221 ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt cctgaatcta
     8281 accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact ccaattccaa
     8341 caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca ccataatctt
     8401 ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa acagctaaat
     8461 gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac atatccgcaa
     8521 gtgcattatt gacaagaatt cctaaaacaa aaccaactaa gactattaaa acagatccaa
     8581 tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta acaacccttt
     8641 tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa tagattttta
     8701 cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc taattcttct
     8761 gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta
     8821 tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc aatgaaacga
     8881 ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac ccaaaagtta
     8941 aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat
     9001 acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa atcaaaaaac
     9061 tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag acatataaga
     9121 aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta aaattaacag
     9181 ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca cagatcattc
     9241 tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa ggagaaaatt
     9301 tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa
     9361 tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca gcatttaaac
     9421 taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact tatcagcaaa
     9481 gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa gacaaataca
     9541 aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa ttcatggata
     9601 aaacaatccg taaagt
//
LOCUS       DAFGFZ010000108         9508 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000108 DAFGFZ010000000
VERSION     DAFGFZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.110"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..2122
                     /locus_tag="LR726_002345"
     CDS             <1..2122
                     /locus_tag="LR726_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217389.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="LR726_002346"
     CDS             2119..2739
                     /locus_tag="LR726_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217390.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="LR726_002347"
     CDS             2853..2990
                     /locus_tag="LR726_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217391.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="LR726_002348"
     CDS             3432..3812
                     /locus_tag="LR726_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL6217392.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3794..4708
                     /locus_tag="LR726_002349"
     CDS             3794..4708
                     /locus_tag="LR726_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL6217393.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHN
                     HIVINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKI
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="LR726_002350"
     CDS             4948..5637
                     /locus_tag="LR726_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217394.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLL
                     GCIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="LR726_002351"
     CDS             complement(5758..5844)
                     /locus_tag="LR726_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL6217395.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            6481..7425
                     /locus_tag="LR726_002352"
     CDS             6481..7425
                     /locus_tag="LR726_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBL6217396.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            7446..7982
                     /locus_tag="LR726_002353"
     CDS             7446..7982
                     /locus_tag="LR726_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL6217397.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEET
                     ESIEKHTKKKQKWWQLWK"
     gene            7998..8168
                     /locus_tag="LR726_002354"
     CDS             7998..8168
                     /locus_tag="LR726_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217398.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8482..8943)
                     /locus_tag="LR726_002355"
     CDS             complement(8482..8943)
                     /locus_tag="LR726_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217399.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            9076..9450
                     /locus_tag="LR726_002356"
     CDS             9076..9450
                     /locus_tag="LR726_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBL6217400.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3664 a         1493 c         1493 g         2858 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat tcttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatatccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atataaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga aaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgaag actctcctcc
     1441 tcaagctcct tataaaactc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgcccaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 gttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     3301 atacggaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtat aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag cgatccttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcagtc agctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc tcctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa cagttaaagt aagtcgaacc acttcatgta gccgagcaat
     3841 caattacgca gaaccacgag caaccgtcaa aacaggaata aactgtgaca ttgattatgc
     3901 caaaagtgaa atgaaacaaa tacgcatgct ttatggaaaa gacgatcgtg tacaagcaca
     3961 tttattgatt cagtcctttc gaccaggaga aattaccgct gaaaaagcca accaactagg
     4021 gaaagaatac gctgaaaaaa tcgcgccaga ataccaaatc gcgatttaca cgcacacaga
     4081 taaagagcat atccacaatc acattgtcat taattcggtc aatttagaaa caggaaaaaa
     4141 gtttcaagca catggtcaag cattcctaga taagtgttac gatatcaacg acgaaatttg
     4201 tttaactcat ggtttaagta tcactgaacg aggaaaaaaa gaagaaaaac gaaccatggc
     4261 tgaaatcaaa ataaaagaaa aaaatgaacc tgtttggaaa gatgaaattc ggtttgcgat
     4321 tgatcaaacc atgaaaaaca agaaaaccag aacctatgac caattttgtg attccctcaa
     4381 aacattcggc attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttcttgct ccctacacac aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag atcagcgaaa taaagaacaa aaaaaggcgt
     4861 tcattcaacg atcaatggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt tccaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttcttgcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt aggcgtttta gagggcaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcacgta ataaaaagaa aaaagagcaa
     5221 gccttagcac aaaaaataga aaaatttacc gcttgttatg aggacatgaa tgatattttt
     5281 gtgaagcaag aaaagcgttt gacggataaa aatatggaaa cactcactcg actttatcgg
     5341 aatttgccag aagaaccgca aaaagaatac caagaaaaat atgaacaagt aaaaaaggac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctacgaaa acctgtttca acaagcaact aacatacaaa gtccaacgaa aaaagaaacg
     5581 gcattaaatt atctagaaag tgtcaaggaa tggttagatc agcaacagca aaattgaatt
     5641 tgatcggcaa tcatgttaaa ctaaacatgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactt ctataagaag
     5821 ggggcaaatg aacaattcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaacttgtgt ggtgttttta agtatcgctt
     5941 attttagggt tgttagggct tgccctgacc gcctgtaagg cgctttaaag ctagataagg
     6001 atttttatcc aagactgtct taaaacggct tagataagca ttcacaaccg ctcaaaggcc
     6061 tctttaccaa catgttttta gcaaaggagc ctttaggcga ctgcgcagta caatgtgaga
     6121 gcgtagcgat cacacctttt tggcgaccgt aggaagcaag gttttggatt ggcagttttg
     6181 aaaaatgcgc gaacctgttt aaagaaaaaa gaagtggttt ttaggcggga aaattagccc
     6241 ccctcttttt atatctcttt ttaaacctct ttttaaacct ctttaccgta agctgtgtcc
     6301 ttactctacc aatggataca gaatgttatg aaggacattt tgtctgttta tgaaggacat
     6361 tttgtctgtt tatgaaggac attttgtctg tttatgaagg acattttgtc tgtttgaacg
     6421 tcaaatatga atgtcttgac atttaaaaaa tgtcattcat aattggattg aggtgattat
     6481 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
     6541 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
     6601 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
     6661 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
     6721 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
     6781 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
     6841 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
     6901 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
     6961 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
     7021 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
     7081 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
     7141 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     7201 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
     7261 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
     7321 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
     7381 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
     7441 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     7501 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     7561 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctagc aacttaggta
     7621 accaaaggtt actagatagg ttgcatgagt atgaaaaaga caacgataaa aacatttata
     7681 tatcaagcaa aaacgtggtt gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat
     7741 ttttacgaga acaactttcc cttaaagagc aaaataattc taatatgcaa aaattactcg
     7801 accagcaaca acagctcacc ctacaggcaa atcaacagat cgaaaaactg caagaacagt
     7861 tacaattagt ttatacggaa gaaactccag aagatgaatc aactactatt ctttctgaag
     7921 aaacagaatc gatagaaaaa cacacaaaga agaagcaaaa atggtggcaa ctttggaaat
     7981 aactggaggg aatattcatg acaagaaaaa atcttctatt cggttctatt ttactaggaa
     8041 ttctaggctt cagtccgatg ttattgtcag tgatcaataa agggaataaa catggtgaat
     8101 tgaaattgaa tccaaattat tacaacagtc ctaacgaaga attgtttgta aaagaaacgg
     8161 ataaataata aaaaagactg ctctttcgag cagccagtta aaaaaataat gagagtttat
     8221 tcttgaaaaa gcaacgcgct tataaagcgc taccaaaatt ttatcatatt ttataaaaaa
     8281 taacaatcca gtaaaaaatg actggtaaaa attcacaaag aaacggaaag aagcaactgc
     8341 agaaaatcaa gccttaaaaa gagcttttga cctagaccaa ttaaaaatca caaagtgagc
     8401 ccctgtatcg attgatacag gcgctactta gcatactgta cattatgtca actatgcaat
     8461 taaataacta actacttcat attagcttaa taaaataatc aaaatcacta ggggaaaaaa
     8521 tattgtataa aaaaattttt cattttttaa ataagaatag taaatataaa aataatttaa
     8581 gatatctatt actattaatc cagatagagc aaaccaagta aatattccgt gtgtaaagct
     8641 aaaaaaattc atttgaatca attcaaataa acctaatata gtaacaaata tattaaaaat
     8701 aatagctgtc aataccagta aattattttg tttatttttt aaattatctt catttaaaat
     8761 agaatttatg ttagtactat aaaattcaca aatttttttt agtgtctcta tatctggtgt
     8821 tgttttgtta ttttcccact ttgaaatagt ttgacgtgta acattaaatt ttcttgccac
     8881 atattcttga gtataccctt ttttcactct taattctctt agcttagatc ctaaatcctc
     8941 cattttttct cctatgtaaa cgattattta cataaaattt tatcataaaa atactaacgc
     9001 gccaaaaaag ttctttattt tataaaacac actaaaataa aataattcta gtaaaaaaga
     9061 taggagaaat aagttatgaa aaaattttta tgtctttctg ctttaatggg tgtcttatta
     9121 actagtggag gaatagttag tgctacagaa gctttaaatc tagatgtacc cgaagaccat
     9181 accgccattt acggaggagg aatggaagct acagaagaag gattttcttc tagtaaattg
     9241 agatatgctg ctggcggcgg agatttcaac tgtggggtta atggatttaa agtttatgca
     9301 aattactacc atgctagagc taaacatagt gcaacagcta aaaatggacg aggcggtcaa
     9361 gtacgatcag ttcagaaagc tggagtaaga gcttatgcaa catgcaatgc cacacttact
     9421 ggaaatactg gttggtggaa tgtttattaa aaaagagagt ttaaactctc ttttttattt
     9481 acatttgaaa ggaaattcaa aatatgtt
//
LOCUS       DAFGFZ010000109         9348 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000109 DAFGFZ010000000
VERSION     DAFGFZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.111"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..254
                     /locus_tag="LR726_002357"
     CDS             <1..254
                     /locus_tag="LR726_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL6217401.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(549..1682)
                     /locus_tag="LR726_002358"
     CDS             complement(549..1682)
                     /locus_tag="LR726_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6217402.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1673..2341)
                     /locus_tag="LR726_002359"
     CDS             complement(1673..2341)
                     /locus_tag="LR726_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6217403.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2797..3783)
                     /gene="lacD"
                     /locus_tag="LR726_002360"
     CDS             complement(2797..3783)
                     /gene="lacD"
                     /locus_tag="LR726_002360"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL6217404.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3796..4722)
                     /gene="lacC"
                     /locus_tag="LR726_002361"
     CDS             complement(3796..4722)
                     /gene="lacC"
                     /locus_tag="LR726_002361"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBL6217405.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4735..5250)
                     /gene="lacB"
                     /locus_tag="LR726_002362"
     CDS             complement(4735..5250)
                     /gene="lacB"
                     /locus_tag="LR726_002362"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBL6217406.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5269..5697)
                     /gene="lacA"
                     /locus_tag="LR726_002363"
     CDS             complement(5269..5697)
                     /gene="lacA"
                     /locus_tag="LR726_002363"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBL6217407.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5717..6490)
                     /locus_tag="LR726_002364"
     CDS             complement(5717..6490)
                     /locus_tag="LR726_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL6217408.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6655..7134
                     /locus_tag="LR726_002365"
     CDS             6655..7134
                     /locus_tag="LR726_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6217409.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7191..7493
                     /locus_tag="LR726_002366"
     CDS             7191..7493
                     /locus_tag="LR726_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBL6217410.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7515..8972
                     /locus_tag="LR726_002367"
     CDS             7515..8972
                     /locus_tag="LR726_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL6217411.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
BASE COUNT         2671 a         2021 c         1634 g         3022 t
ORIGIN      
        1 gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg aactgcttag
       61 ataattcact tatggagaat ttttttggct tattaaaaca agagattttt cacgggaaag
      121 tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat tactacaata
      181 atgaacggat aaaacagaaa ttaaactggc aaagtcccgt ccaatttcgt aaaacaaccg
      241 ttacggttgt gtaaaaatat cattttagag catgaaaaaa agagtggatt tctccactcc
      301 aaaaaagtct aacttttggg ggtcactaca tttttgatcc tttttatttg attaattata
      361 tccagctgga taataatttt gggggcaaca tttctatctt actattatct tcctaaagta
      421 gtagactttc ttattgggaa aaattacaac aaaaaggagt ggcttagttg acaactcctt
      481 tttgtttaca aatcaattgg atatctataa attgtatttt ctaacgaatt ttatagacat
      541 ctgatttgtc aaattaagct agacaatatg tctttatcta tgcagctcaa aaatacttcc
      601 aatggtgttc gataatttaa tgattttctt ggaatatggt ttcttttcga ggcaacggat
      661 tgaataaagg tttcatccgt gtccctaaag tccattgttt taggtaagcc atcctgcttg
      721 agcccatgca ctcggtactg atgagaaaaa attgatgcct gtttctcctg aggaatgcca
      781 attccctgat cttttacttc aataatcacg ctaccctttt ggtttgaaat ggtgacaatt
      841 accagttttc cttcaccata tttgattgca ttgtcaacta aattcgaaac cgcccgataa
      901 atccaacgat ctcgtccatg aatcagggtt ggttgatctt cagtaaacgc aaacaaaaga
      961 ttcggataat tcattttgta ggcatcacaa acttccgcac agatcagtga aacgtctacg
     1021 acttccgtgc taagatcctc tgagttattg gataatgtca aaatatcgtc aatactgtcg
     1081 gtcatccgat ctaaaagcat taaatttcct tgatcggcat gttgccgttc tatttccaga
     1141 ttcgtacgca aaatagcgat ggcatttttc tgctcgtggg tcagataact atttaatcgt
     1201 cgataatcct ccaaatgacc atcgaactta tcctttagct tattaaaagc cgcatttaat
     1261 gcctcgtcct tcacagtatt tttaggaact tgatcaataa acgttaaatc acgcgtaatc
     1321 gctaagattt gattactgta aattttgcgt aaaaccaacc aaagaataaa cgttaataac
     1381 atgacggcag aacaaataac cagcaccatc gctggtaaat atttaaatag ttcgtcctgg
     1441 taagctttat ctatcatcga aatagtcata aattgattgt tggaagattt cgaagcttcc
     1501 atctgcgtct ctgcttgaac cgtcttaccc tcttccataa aataaacatc ttcacctaag
     1561 gtagatgcga tactgattcc ctccaaatta tttctcagaa agaagaaact cactaaaatc
     1621 acgcctgtca ccaacagtga aaaagccagc atcgttaacg tcattttaaa atttaatagc
     1681 atagctggta cccctttgct cgaatggttt ttaagacctc tttttcagct gcttcagcca
     1741 gtttcttttt tagacgagcc agatgcactc gcagaaccga cgaaaaagga tcaaaatctt
     1801 catcgtagac atgttcagaa atttcctcag acgaaacaac cgctggatga cgctgagcga
     1861 tgcacagtaa gatatcaaat tccttgggtt tcaggaccac ctctgtcttt tgatagctga
     1921 cccttcgacc taatggatca atcttcaaag caccaattga aatctgcggc agcgcacgtc
     1981 catgataacg acgaataacc gcgcgtattc gtgcttttaa ttctagtaac tcaaagggtt
     2041 tgactaaata atcatctgct ccaaaatcca agccttttgc tcgttcttca atctcatcac
     2101 gtgcagtaat aatgataact ggactttcga cttcttcagc acggagactt tgtaaaacag
     2161 ataatccatc ttttttaggc agatttaaat ctagtagtat cccatcgtat gaattgatga
     2221 aggccttctc ttctcctgac tcgccatcat aagctacatc tacagaaata ttctcctgct
     2281 ctaggccctt ttttacggat aaaaccagct cacgattgtc ctcaaccacc agtacacgca
     2341 tgtatattca ctcctcatac ggatttagta attacactta tgctttttag catagtacac
     2401 gctcttcttc aaagtccacg ttctttttgt gaagatttga agaaattcat acattctgtt
     2461 aaaaaacaca taattaagaa tgattttact gtttgaaaat acctagaata gaaaaagact
     2521 ggaacctttc ctccagtctc actactttct atttgcttgg ttttcttgta tatacttttc
     2581 aaagttcgtt ggtttatgac caataagcct cgttagaata ctgaaaattg ccatcaagtc
     2641 attaagtacc acatgtaaga aaatctctct aaagaaaaac cccttcgttt taactcactt
     2701 actattttag aattcgttcc cctaataatg cagcatgatt ttacgcttta tgtataaaaa
     2761 aagactgcaa gcaattttgc ttcacagtct gatttcttat gatagtgagc ttgttaacac
     2821 ttccaccggt gtagcagttt ctttcaatac ggcatttaat tcttcaatat ttttacgccc
     2881 ttgcgtattc atccactcca tcgctgcttc ttttccatca acgatatagc tttctacacc
     2941 atctgcccaa gtcgcacggc cgcaaagtac accattgaag gttgagccgg catttttcgc
     3001 gaagcgcagt gtttcttgga acaactctgc ttttacaccg gcgcttagga aaataaatgg
     3061 caggttagtg gcttcagcct gttccttgaa gaagttagcc gcttgtgcct ttgtgtacac
     3121 gacttcgtct gtcccatagc cttccacata attcatattg acgggtactt ccacctttaa
     3181 cacatctacg ccataacgtg gatcagaaaa ttccttcatc atatcgttga ctttatgagg
     3241 tttcttaatg gcgtattctt tcgaagtcgc atcagagatg ttcgcgtcat aagaaacaag
     3301 ctccaagaag aagggtaagt tttcagcctg acattctgag ccgattcgtt caataaaggc
     3361 atgtttttga ttattgattt cttctgcttc atcgacatca tagtaaagta ggaacttaca
     3421 agcatcggcg cctgcttctt ttaaacgttt aactgaccag atcgataata aatctggtaa
     3481 acggccggga gttgacgcat cgtaaccagt tttttcataa gctaacagca agccggcatt
     3541 ttttgctcta acgtcagctg ctggcaagcc atattcggga tctaataaaa tcgatgaggc
     3601 atatttcgtt aattgtgaag aaacaatttc tttaaattca acgatttctg attcttctgc
     3661 ttcgctgccc ttgaattttg tgatcatctt cttcaaagcc ccgcgttgat caattgctaa
     3721 ggccccaatc actccatctt cattgcatag ttgcttgata tattgacgtt tttcttcgtt
     3781 catttccgta tcctcctaca ctttctccac taagatttga tcatatagtt tttgataatt
     3841 ggctaattgg acatagccag tcgtcgcttc ctgagcattc agcattccta aaacattacc
     3901 ctgttttaac acggcttcat ccgaaagacc ttgttgaatc gcagcggtca atccggcaac
     3961 ggtcgcatca ccagagccaa ctggattgac gacatcaatc tttggaattg tcacgcaata
     4021 aaaagtctcg tgatgtttgg caaaggctcc gtcgcccccc attgaaacca ctatccattc
     4081 aatttcatca aacatcgaat ctgacaaggc ggcttttaaa tctgataatt ctgttgagat
     4141 ttctcgtcct agcagaccag ccagctcttc ggtattgggt tttattagct taggctttac
     4201 actgcctcgc aacacttccc ttaatgctgt acctgaacaa tctaaaacga caggctttgg
     4261 ctgacaaact tgcagcattt gtcgataaaa atcagcaggc aatccttctg gcaagcttcc
     4321 tgaaaaagaa atgatttcgg ctttaacagc taatttttcg aaatgttgca agaacgccct
     4381 tgcttcttgt tcagtaataa caggtccgtt ttctaggatt tccgtctgtt ttccgtcatg
     4441 taaaacagcg atgcaattac gtgtctctcc gctgataggt aaaaagtcat gagcaatctc
     4501 attttgagtc aggtcctttt taatctcttc acctaaaaaa ccgccgatta aaccactagc
     4561 aatcacctcc gtatctaatt gcttcaagat acgtgtcaca tttaggccct taccgccagc
     4621 tgttttgcga acatttctta ctcgattgac tgtgtcgagc ttgaaggttt ctaatggata
     4681 agaaatatca atcgatggat tcatcgttat tgttaaaatc atttgtccac ccgcttaatc
     4741 gtggtattcg ccacgatccc atttctcgat aaattcatcg aagaaatgcg gatcagactg
     4801 cttgtcactg gctttttcga cgtgatcgat tttggcgatc aattttttgt tttcttcagt
     4861 ttcttgataa tcggcattga tgaaggcctc taatatgtca cagatcaaaa attctcccgt
     4921 aatccgagcg ccaaagccga tcacattggc atttagttgt tccttcgcat agatagcagt
     4981 cgtcatatcg cgaaccagcg cactgcggat accaggtact ttattgacag cgttgttaat
     5041 cccaacacct gttccacaca aacaaactcc tagatctgct tctccgctag tcacggcctc
     5101 tccgactttt tttccgtaga tcggataatg ggttctggta aagtcatagg tccccatatc
     5161 caacacttca tgtccttttg attttaagaa atctgatact gccatttttg tatctgtgac
     5221 gatgtggtca cagccaattg cgattttcat ttcattcatc cttcctaatt acgccatttt
     5281 atttaacatg tcgacacgta cttgatgacg accaccatca taatttgctt gtagaaattc
     5341 cttaacaata ttcaacgcca actcatcgcc aacaattttt gatccaagtg tgatcatccg
     5401 agaattatta tgtttacggg tcatatacgc actgcgctca tccgaaactt ctgctgctac
     5461 cattccttta acctttgttg ccgtgataaa gctgccagcg ccgtagccat caaccgcgat
     5521 tcctaaacta ccttcgtttt cattcagatc atttgcgatc gccaaagttg attccacaaa
     5581 atcttctgaa gccgtttcac tcttatctac tactgtaata ccttctttga tcaatttttt
     5641 cttgattaat tcctttaatt ccatgccgtc actgtctgaa ccaattacta cacgcatttt
     5701 tgtttcctcc tattttttac tgaatgattt tagtgaattg ctgataataa ctcttcagct
     5761 cggctgaaag agtagaatcc gtaaataatt tgtcgatctt gcgcaaatca tagaaattat
     5821 aaaaatcgct atggtcgaat ttgtttgcat ctgaaaccaa acatttttct gccgcattat
     5881 ctaaaacaat tttttgcaag gtaccttctt caatactgaa attcgaaacc tccgaaccct
     5941 tcaatccatt cactccgaca aaagccttct taataccgat tttttgaaca gcttcattgg
     6001 caattgaacc gacaaaggct ccagtctttt cccgatatag cccaccgatc aaatacagtt
     6061 caaatctttt tatttcactt aatagattga aaaccggcag actattggta acgattcgga
     6121 gagatgacgg cttgatgtat cgtgtcatta attcgatcgt tgtacccgat ccaagaaaaa
     6181 tcgtgtcgtc ttcttgattg atatgagctg ccgcattttt agcgatgtat tccttctctt
     6241 ttgtatgcag cttctttttt tcagtatgag aaagctcatt tgcttctata gaagtcgaca
     6301 tttccagata atcgattttt tgtgcaccgc catgaacgcg taaaagcttt ttcttctgcg
     6361 cgagttcctc tagatctcgg cggatcgtca tatctgaaac attcagtact tcctgaataa
     6421 atttgactgt tatcgtccca tagcgatcac acatcgctaa gatcgaacgc tgtcgttctg
     6481 ttttcagcat attctgtacc tccacgacct tataataaaa catttacgaa catttttcaa
     6541 cacatattgt tcgtttattt tcgtttatgc gttgacaatg attttgtaag cggttatact
     6601 caacttgtaa acatataaat aaacataaac gaactaataa aggagctgtt ttgtgtgaat
     6661 gaattctttt tcaaagacac tgtctatgtt tctgacaagc agtcgcagga agaagtgttc
     6721 gaggaagtct atcaagatct gttaaagaaa gaattggtaa ccgaagattt tttggagaac
     6781 cttttagaac gggagcgtaa ttatccgacg ggattagatc tctcacctgt ctctaaaata
     6841 ttgccagata tcgctatccc tcataccgaa agtgagtttg tgcgaaccac ccgtatcatt
     6901 ccaatcaaag taaaaaatga aatccctttc tacaacatga tctcgccaag cgatcagatt
     6961 tccgcttcat ttcttttcat gatcttgaac gaaaatggtg aagcacaggc tggcatgttg
     7021 gctaatatca tggatttcat taattctgtt gacgaagaaa aactgttttc gtttttcaaa
     7081 ttagaggaca cagaagcgat ttatcatttt ttagaagaac atttttatat atagaagtct
     7141 ctgcctaacc gcaggataaa aaataaactt attttaaggg gagaattatt atgatcaaaa
     7201 ttttagcagc atgtggagca ggcgttaatt caagccatca aatcaaaagc gctttggaaa
     7261 ctgaattgaa aaatcgtggc tatcaagtca cctgtgacgc cgttatgatc aaagatatca
     7321 atgaagaaat gctgtctcat tatgacattt tcgcacaaat tgccaacacc gacttgggtt
     7381 ttgacattaa catccctgtc gttgatgcag gtgctattct ttatcgtatt cctgctatgg
     7441 ctgagcctgt ttaccaaagt atggaaagcg caatccaatc attaaacaaa taggactaaa
     7501 gaaaggaaaa gcatatgaat acaattattg atattgccaa taaagtattt acccccctaa
     7561 ttaatttagg tgcggcaccg ttaatgacca tcgtattaac tgttattgct ttatttttta
     7621 aagtaaaacc gtcacgcgct cttgaaggcg ggctcaaatt agggatcgcc ctgactggga
     7681 tcggagctat tattggaatt ttaactactg ctttttcaga cgctatgaca gcttttgttg
     7741 aacgcacagg tatttcatta aacattacag atgtcggttg ggccccacta gcaaccatca
     7801 catgggggtc gccatacacg ctttattttt tactaattat ggtgatcgtc aatattgtca
     7861 tgttagcaat gaaaaaaacc aacacattag atgtggatat tttcgatatt tggcatttag
     7921 caattgttgg tttgttcgcg atcttctgcg gtgctaatct gtttattgct tctgcattag
     7981 ttattttcat tggtgtatta aaaatttgga actccgactt aatgaaacca accttcaatg
     8041 acttgttaaa tgcgccagac agcaacccga tgaccacgac tcatatgaac tacatgatga
     8101 atccgattat tatggttttc gataaaattt tcgacaaaat cttcccttgg ttggataaat
     8161 atgatttcga tgctgccaaa ttgaatagta aaattggctt ctggggatca aaattcgcga
     8221 ttggaattta tttggggatt tttgttggat tactagcagg acaaacacca acgcaaattt
     8281 tctcgctagc ctttaccgct gccgtctgtt tggaattgtt ctctttgata ggcgcttggt
     8341 tcatcgcagc cgtagaaccg ttatcacaag ggattaccga ttttgccaat aaacgactaa
     8401 aaggacgtac catcaacata ggtttagact ggcctttcat agcaggccgc gctgaaatct
     8461 gggctgccgc taacgtatta gcaccaatca tgttgctaga agccatcatc ttaccaggaa
     8521 ataaattatt gccactaggc ggaatcatcg caatgggtgt taccccagca ctattggtcg
     8581 taactcgtgg aaaattaatc cgtatgatcg taatcggcgc gatcgaatta ccattattct
     8641 tatggtccgg caccttgatc gccccgttcg ttactcaaac agctaaagca gtcggagcct
     8701 ttccagccgg cttgaacggg tccgccatga tctctcatac aacgatggaa ggcccgattg
     8761 aaaaattcct aggttattta gtcggtaatg cttctcaagg acaaatcgaa tttgtgatat
     8821 acgctgcctt agcattaggt gcataccttt taatcttcat ttggtacgct cgtcaaatga
     8881 aaaaacgcaa cgctgcatac gcagctgaaa aagaagagaa tgcatctccg gcaacggcgc
     8941 ataatatgac ttatgcagcc gaagctaaat aagctaaaaa caccccgaat gtctttagac
     9001 ttcggggtat tattttacct ataaaaaata aatagatgta aaatccattt caaaaagcat
     9061 agcccatctc ttgccacacc caataaagaa attatcaacg tttatgcttt ttttaactcc
     9121 tttaagcttg cctttgttgg tattcctcaa cgattctttt tgtgttcttg tttgttttgt
     9181 gctttttgac tttttgttgt ttaattgcga attccttgat ttcttttttc ctgaatcgct
     9241 tcgcatcccg cggttatgcg tgcgatttca tcattgcttt ttctgatcta actcgtttca
     9301 tgtcaaagag cttcagctga cttagtgtcc atggttgttt tgatatcc
//
LOCUS       DAFGFZ010000110         9087 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000110 DAFGFZ010000000
VERSION     DAFGFZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.112"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            126..944
                     /gene="purR"
                     /locus_tag="LR726_002368"
     CDS             126..944
                     /gene="purR"
                     /locus_tag="LR726_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL6217412.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1447..2820
                     /gene="glmU"
                     /locus_tag="LR726_002369"
     CDS             1447..2820
                     /gene="glmU"
                     /locus_tag="LR726_002369"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL6217413.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2914..3885
                     /locus_tag="LR726_002370"
     CDS             2914..3885
                     /locus_tag="LR726_002370"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL6217414.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3891..3993
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4059..4628
                     /gene="thiT"
                     /locus_tag="LR726_002371"
     CDS             4059..4628
                     /gene="thiT"
                     /locus_tag="LR726_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL6217415.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4709..5482)
                     /locus_tag="LR726_002372"
     CDS             complement(4709..5482)
                     /locus_tag="LR726_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217416.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5749..7449
                     /locus_tag="LR726_002373"
     CDS             5749..7449
                     /locus_tag="LR726_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL6217417.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7419..7637
                     /locus_tag="LR726_002374"
     CDS             7419..7637
                     /locus_tag="LR726_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL6217418.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7657..8547
                     /locus_tag="LR726_002375"
     CDS             7657..8547
                     /locus_tag="LR726_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL6217419.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8565..8849
                     /locus_tag="LR726_002376"
     CDS             8565..8849
                     /locus_tag="LR726_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217420.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3066 a         1557 c         1905 g         2559 t
ORIGIN      
        1 ataggcttct ttttatttta ataaataccg aactattttc ttattttttc ataaaatgct
       61 cgaatttttg aagaaaaact tatataatgt aaaacgataa aatttcttaa aaggagttgg
      121 aataggtgaa agtacgtcgc agtgaacgtc tagttgacat gacacagtat ttattgaatc
      181 acccgcatga attgatttcc ttaacaagct ttgccgaacg ttatgaatct gctaaatctt
      241 ctatcagtga agatttggga atcatcaaga aaacatttaa agaacgaggg tatggaacct
      301 tggaaacaat cccgggtgct gcaggtggtg tattgttcat ccctgaaatt tcatatgaag
      361 aggcgaaaaa atatattgaa tctctagcag aacgtctatc agaacaagac cgtctgcttc
      421 caggaggata tgtttatctc tctgatttgt taggagaacc tgaactgctg cgtcaagttg
      481 gaagaatcat tgcttctaaa taccttggtg aaaaaatcga tgctgtaatg actgtagcca
      541 caaaaggcgt acctattgcc caagctgtgt cctattatct gaatgcgcca ttcgttattg
      601 tacgccggga ctcaaaaata acagaaggat caacagtaag tgtcaactat gtgtctggat
      661 cttctgaaag aattgaaaag atggaactgt ctaagagaag tttgaaacgt ggttcacgtg
      721 tccttgtagt ggatgatttc atgaaaggcg gcggaacagt caacggaatg aagagcttga
      781 ttgaagaatt cgaatcagaa cttgttggca tcactgtatt tgccgaatca aaattcaacg
      841 gtcatcgcgc catcgatgat tatacgtcac tgctttatgt caaagatgta gatactcaaa
      901 cgaaaaatat tacggttgtg ccaggaaatt atttttcaga ataagaggtt gtgaaaaagc
      961 tgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat tttttcataa
     1021 atttggctta ataccgagaa actggctttt gaacaccgtt tatctagagg ttgtgaaatc
     1081 agcgtcctgt cctgagtatt agctcaataa acggaaaaat tgctcctcat attttgccgt
     1141 ttattgagct aataccgaag gtcagctgat tgaaaaacgt ttaatgaaga ggttgtaagc
     1201 ctaccgttta actctaagtc acaagaaacg atttcaaaaa acagcttttc atgttttatg
     1261 agtagtgtga cttgtgaccg aggagttggc aggagagcac cggttaccaa atagataggc
     1321 ccgttatggt ctatctattt ttttatccag aaaacgctgg gataaaagaa aatcacttgt
     1381 tttgttgaaa aaatgggtat actgggataa actgaaatca gcaaactata tttaggagtg
     1441 acgcagttgg acgcacgtta tgcaattatt ttggcggcag ggaaaggtac ccgcatgaag
     1501 tcaaaattat ataaagtttt acatcccgtt tctggtaaac ctatggtaga acatatcatt
     1561 aatcgggtca gtgaaacgaa accagatgaa gtgattacga tcgttggaca cggagcagaa
     1621 caagttaaag cacaactagg agaacgcagt aaatatgcgt tacaagcaga acaattggga
     1681 acaggacatg ctgttttgca agctgcttct ttcttggaag gaaaaaaagg aacaacgtta
     1741 gttatcagtg gcgatacacc attattgact actgaaacgc tgaacaatct ttttgaatat
     1801 caccaaggaa aaaatgcaag tgcaacgatc ttgacggcac aagcagaaaa cccaacagga
     1861 tatggacgaa tcatccgtga ccatattggg atcgtagaaa aaatcgtgga acaaaaagat
     1921 gcaacaccag aagaagctct tgtacaagaa atcaatacag gaacttactg ttttgacaac
     1981 gaagctttgt tcgatgcgtt aagcaaagtt ggaacaaaca atgctcaagg tgaatactat
     2041 ctaacagata tcatcgaaat cttaaaagaa gaaggccata cagttgctgc ttatcaaacg
     2101 gatgattttg aagaatcaat gggagtcaat gatcggatcg cgttagctaa agcaaatgag
     2161 atcatgagaa agcgaattaa ccagatgcat atggtcaatg gcgtaagctt tgtcgattca
     2221 gcaacgacat acatcgatgc aggcgtagaa atcggtccag acacattgat cgaagcaggt
     2281 gtacaaatcc aaggaaatac agtcattggt tctgattgtg tcatcgggtc acattcaaaa
     2341 atcgtagaca gtaggattga ggatcacgtc gtcattgaaa actcagtcat tgaaagcagc
     2401 catgtcaaga aacatgcaga tgtgggacct tatgcacatt tacgaccaaa agctgaaatt
     2461 ggcgaaaacg tccatatcgg aaactttgtg gaagtgaaaa atgctcaaat cggcaaagga
     2521 actaaagtcg gtcacttgac ttatgttggt gatgctacgc tgggagaaga aatcaatgtc
     2581 ggatgtggtg ttgtcttcgt caactatgat ggaaagaaca aacatcatac gactgtcggc
     2641 gaccacagct ttattggttc tagcacgaat atcatcggac ctgtcgaggt agctaaaaac
     2701 agttcgattg ctgccgggtc aacaattaca gacaatattc cagaatatgc gctggcaatt
     2761 gcgagagcaa gacaagtcaa taaagaagga tatgcaaaaa aactccctta tctaaactaa
     2821 gacttttgca aaatcacttg atttatttaa aaaatatgac tactatttat taggtagaat
     2881 tctagagttg tgaaaaagaa agtggaggtc cccatgtcaa aacattactt tgacccaaga
     2941 ttaaaaattt ttgcgttaaa ttccaatcgc ccattagcag agaaaattgc agcagcagtc
     3001 ggcgtcgagt taggaaaatc atctgttact caatttagtg atggagaaat tcaagtaaac
     3061 attgaagaaa gtattcgtgg atcgcatgtc tatgttattc aatcaacgag cagcccagtc
     3121 aatgataact tgatggagtt gctgatcatg attgacgcac tgaaacgagc aagtgcaaaa
     3181 acaatcaacg tagtcatgcc ttattatggc tatgcgcgtc aagaccgtaa agcacgtgca
     3241 cgggaaccaa tcacagcgaa attagtagca aacatgattc aaaaagctgg tgcaacaaga
     3301 atgctgactt tggaccttca tgcagttcaa atccaaggtt tctttgatat tccagtcgat
     3361 catttgatgg gtgcgccact tattgccaat tatttcttag aaaaaggaat caaaggcgat
     3421 gatgttgtcg ttgtttcacc agaccatggt ggtgtcacac gggcacgtaa attagcggaa
     3481 tttttgaaat ctccgattgc gatcatagac aaacgccgtc caaaagcaaa tgttgcagaa
     3541 gtaatgaata tcattggaca agtcaaaggc aaaacttgtg tactgatcga tgatatgatc
     3601 gatacagcag gtacgatcac attggctgcc aatgctttga aagaagctgg tgcaactagt
     3661 gtgtatgctt catgtacaca tccagtatta tccggccctg ctttacaaag aatagaagat
     3721 tcagctatcg agcgtctagt tgttacagac tcgatctatc ttccagaaga tcgtaaaatc
     3781 gacaaaatcg atgaagtaag tgttggcgga ctaatgggag atgcaatcaa acgtatccat
     3841 gaaaacaaac cagttagtcc attgtttgaa acaaaaaata aataataaat aaactcacaa
     3901 aggggagtcc aattgggctg agattgaatc tatttctaaa cccttcgtac ctgtatcggt
     3961 tatgcgagcg taggaattgt gaataaacag ctgtttgact attttcgaaa gctgcttcct
     4021 cctttgtgac ttttttattc aaaggaggaa gtttttttat gggaaaaaat agagtttggg
     4081 tagaaggaac gattgttgca gcgttagcga tggtgttgtc gttgattccg atccagatcg
     4141 gcagcagttt ttctatttca ttagggcaga ttccattgac tctttttgcg ttaagaagag
     4201 ggacaaaggc agggatgctg gcaggtttca tttggggcat cctacatttt ccgttgggac
     4261 aagtttatta tttaagtgtc gttcaagtgt tgattgagta cccgattgcc tatacttttg
     4321 caggcgcagc agggctcatg gcttcaaatg tccaaaaagg ctatgtagac gataatacga
     4381 aacaaattag aaaaagcatc gtgtttggtg cattgattgg cgcactttcc agatattttt
     4441 ggcattttat tgcaggagtg gttttctggg gagcgtatgc actatggggg atgaatcctt
     4501 ggctcttttc tttcgtaatg aatggtgcca gcgggttgag tacagctatt gtaacgatca
     4561 tcgttttatt agggatccaa caaacgactc cttcgatttt tgtgccagat aaacgacagg
     4621 agctgtaaat aaaaaagaag caagaaactt gagacagaat catgtcctag tttcttgttt
     4681 cctttttatt ttttgataag aaatagatct acttcaatgg cagataagaa agatcggcat
     4741 cttctaccaa ttgccagtgt cgatcggtga cagagtagat taaagtagcg atgatttcct
     4801 cttctcgata ccgggtgcct tttaatttcc aaatataatc ttcaatcaca tagattggga
     4861 tacgatattt accatcgatc aaaccaactt gatcaaaaga aaaaatgatc ccttcctgca
     4921 tcaaattacg catttctttc aataattcca cactgatttt ttcaacaggt gtttcttttt
     4981 tgaaaaaaga tttttttaca ttgatcttat ctgacaagat cgaacttaat ttactagcaa
     5041 aaatcctata aaattcatct aaatagcgat aataaatccg attcagctga tcattaagat
     5101 cgaagtatac aaaattattc tgtagtttat aaaaaaacgg cgaatgaaga tgtgttttca
     5161 tatgaccaaa atataataat tctgaaattt ctaaaggtgt caattctttc agcatcgtta
     5221 aatcagtaaa gtcgatccac ttcaattcat cgaatccacg cctgttacga gaagaggaaa
     5281 agaaacgctg aatattttct gtaccacgga ttactttcag ccctgtatgg gattcatatt
     5341 ctccagcaga agcagatgga tcaagcaaga gcaagttttg aggaaaatgc acgatactgt
     5401 ttgcaaagtc agtagcagtc agcccttttg ttaaaacagc attacttgtg gtatcaatat
     5461 gtacataaac tgactcagtc atcatgttcc tcctcttatt tcctcttaat ctcttttatt
     5521 ttacaacatt tgcattaaaa aagcttgttt cttttatttt aaaattccat tacaaaatca
     5581 cgttttcatt gaaaacactc tcattttcaa ttaatttttg tgatttttgt atttttttat
     5641 ttaaaggagg ttttatgaat tgttttctta tggaatctac aaagtcgaat acgagactca
     5701 tgaccttttt atgacgtact ggtatacttg aagaaaaagg agagcgcgat ggagataaaa
     5761 aaagtttact tttcacagaa aatgtcgctg tattcgccta tccagcaagc aaaacaaccc
     5821 cttccatttt ttcaaagtca tcaagaaagt agagacgctt tttggcgttc tgcaaaaaag
     5881 caatgtgctt ttaaagcagt atcgaaaaaa tataccgtct attttactat tgctaaaagt
     5941 agaggagatg ttatttttaa taatctgttg attgaaggtg aaacttttga atggaaaaaa
     6001 ttttttcggt atatggaagc ttgcgctcgt ttttttatta aagaaaactg ttgtttttta
     6061 tttcaatctc ctctatcaga agaatggttg cgtatttttc ataaaaacgg ctatcaagga
     6121 accacacaat taaataaaaa gctggtctat catacagcac ttgttttagg tggaggagga
     6181 gcgcacggtg cttatcagat tggtgtgtgg caggcgctga aggaacatga tatcaacttt
     6241 gagattatca caggtacctc ggtaggtgcg ttgaacggcg cattgatttt acaaggagat
     6301 atgaaaaaag ctgtgaattt atggaaaaag ctgtcgacaa gacaagtgct tgcgttacca
     6361 gaaatggctg cagttgaaga ccttcgtgaa cgcttctatc gagaaagacg ccaaatgacg
     6421 aaaacagcac taattgaagg cggggtttct tctgctccat tggaaaagat ggtcaaagac
     6481 aatttggatt tgtccttact caaacataat cccaaaatac gtttatatac ggtgtctact
     6541 aaactgccag aactagcgga agtagtgact gatatccaac agacaaaaac agaagaaata
     6601 ccagattgga tactcgcctc tgcttcattt tatcccgcta tggcttatcg aaagatcgga
     6661 aaaaataaat atgcagacgg tgggtatcgc aacaaaatac ctattgatat tgcgattaac
     6721 aaaggagcaa cagaggcatt tgtcgtagat gtgcaagggc caggcccagc gaaaaaaatt
     6781 cgtatgccag acatttttat ccattggaaa tgtcaaacat tgtggacact aggcagcttt
     6841 ctgctttttg atagccaaag gaaccaatta aatttacaat taggctatct ggaaatgaaa
     6901 aaacgattag gctattacta tgggaactgg tatacatttc attctgttaa acctgcaaga
     6961 acatactggc gaggattttt gtcttactta acaaaagaaa ttcagttaga attatctttt
     7021 ttcaaaagca taaaattttg gcaaaaactg cgtaatttat ataagaacag ggtcgtacca
     7081 gagacatgcg gattggcgat gctagaactt ttagcaaaaa aacatttttt actgcccaat
     7141 gaaatctatc aagtagacat gatgattcaa atgatctgcc aacaagatat aaaaagtatg
     7201 ccggatgatt tattggcaca aattggtcaa ttaagtactg aagaatggcg aaggtatcga
     7261 aaataccagc aaaaaataca aaatgagcgc acgaaaactg tttattttga ttatctgcta
     7321 caaggaaaac gcaaggatcg tttgcagcat gaattgctta agcaaccagt tgacacatta
     7381 ctgatcctat atttatatta tctcaaggag gaacaaccat ggcacaagaa ttttcatatg
     7441 aaatcgtaga agaaatcgct atattgtcag aaaacaacaa ggggtggcgt aaagaactga
     7501 acctagtcag ctggaatggt cgcccgccaa aattcgatct tcgagattgg gcgccagacc
     7561 atgaaaaaat ggggaaaggc ctgacattga cgaacgaaga gtttgaacaa ctgcaaaaag
     7621 caatagaaaa tatgtaatga ggaaggaccc tgtcgaatga aaagtatgac tggttttggc
     7681 aaagccatcc gcgaaacaaa agcgtatcag ttagatattg aaataaaaag tgtcaaccag
     7741 cgttttctag atgttcaaat acgaagtcct aaaatcctga actttgttga aaacgatttt
     7801 cgtcagctta ttaaacagca gttaagccgc ggtcgaatcg aagtatttgt gaatctagct
     7861 tatattggca aaaacgaaaa acagatcgtc atcaactggg aattgattga ttcacttatc
     7921 actcaattga cagacggaat tcaacaacga tacggtagtc aaaatcaagt gtcaattagt
     7981 cggatggcgg aacgcttcgc gctggacgaa aacttcgtaa cggtagaaga aaaaccggaa
     8041 gagagcatgg aagaattggc acagttagct ctagatacat tgaaaagcgc acttgattct
     8101 attgaggcga gtcgagaaaa agaaggtcaa gctttagctt tagtgttgaa aaagaatact
     8161 gaagaattca aagaagtact aaaaaaactc gatagttttg tagaaatcta tgaaaaggaa
     8221 tttcaaataa ggtatcaaaa aaagctggag gaatttcttg gagcgaaagt cgatgagcaa
     8281 cggttgttga cggagctagc catcttgttg gaacgcggag atattcacga ggaactggat
     8341 cgtctggcga tccatgtaca aaaactagat gaacttatcg aagcacaatg tcctgtaggt
     8401 cgggaattag atttcctgat ccaagaaatg aatcgggaaa tcaacacgat cggttccaaa
     8461 tcaaatgcaa tcgaaattaa aaatcaagtc gtccagtcta agacgatctt agaaaaaatc
     8521 agagaacaag tacaaaatat tgaatgacga aagaggtaga gataatgaaa tattatgacg
     8581 taacgttcca cgaattaagt ggaaaaactg tggttaaacg ggatatccct tctgaaaaaa
     8641 acggctttga cgtatggaaa gatgcctgtg cagactataa tgagaacgaa ctgtttattt
     8701 tgatcaacga cggtgcttac gtgacgatga atcggaaatt tattgttcga attgacacag
     8761 aagaggtaga agatccgaca gaaaaagcac gttctcgaaa agatgagatc atgggtgtag
     8821 tgaatacttt gtccaatatg ggcttttagc aaggatgctg aacaaagaaa tacaataaat
     8881 aatcagaatt ggatttcttc gcttagcttc tttgctctag gaatacattt aaaggttgtg
     8941 acaaaattcg tgtcacaatc tttttttatt ttgaataagt gatgttcaaa aaccattttc
     9001 tcggtattaa gtaagcgccc catagaacag taccttacta acaaatgcac ctcaagatat
     9061 acttgagcta tcaagaagtc tggaggt
//
LOCUS       DAFGFZ010000111         8858 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000111 DAFGFZ010000000
VERSION     DAFGFZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.113"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..286)
                     /locus_tag="LR726_002377"
     CDS             complement(<1..286)
                     /locus_tag="LR726_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL6217421.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTI"
     gene            complement(292..1155)
                     /locus_tag="LR726_002378"
     CDS             complement(292..1155)
                     /locus_tag="LR726_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6217422.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1169..2065)
                     /locus_tag="LR726_002379"
     CDS             complement(1169..2065)
                     /locus_tag="LR726_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6217423.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2240..3529)
                     /locus_tag="LR726_002380"
     CDS             complement(2240..3529)
                     /locus_tag="LR726_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL6217424.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3501..5018)
                     /locus_tag="LR726_002381"
     CDS             complement(3501..5018)
                     /locus_tag="LR726_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL6217425.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5216..6106
                     /locus_tag="LR726_002382"
     CDS             5216..6106
                     /locus_tag="LR726_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL6217426.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6179..7003)
                     /gene="nadE"
                     /locus_tag="LR726_002383"
     CDS             complement(6179..7003)
                     /gene="nadE"
                     /locus_tag="LR726_002383"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL6217427.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(7015..8487)
                     /locus_tag="LR726_002384"
     CDS             complement(7015..8487)
                     /locus_tag="LR726_002384"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL6217428.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         2738 a         1831 c         1449 g         2840 t
ORIGIN      
        1 cgattgtctg ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac
       61 taaggaacaa gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat
      121 ttcctgatcc ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca
      181 cgatagtaat aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag
      241 aaaccacaat caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt
      301 cattccagac atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag
      361 taatggcagc attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg
      421 ccctgtaaat aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat
      481 caatggataa agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag
      541 agtaggtttg ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc
      601 aatgcgtgct gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa
      661 gataccaaat gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc
      721 aagcaaattg acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt
      781 cagcaaaacg aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata
      841 ccccgcaaga gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt
      901 atttttgatg taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt
      961 atagctatct gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga
     1021 agaaataatc agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat
     1081 cgttcgaatg atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga
     1141 gctttctggt gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt
     1201 acacattagt ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg
     1261 atgcatagcc caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg
     1321 tttctgtctt aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca
     1381 tcgaaccaat cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg
     1441 taatgttaaa aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag
     1501 agggagagat tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc
     1561 aaacagtcat gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga
     1621 tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca
     1681 tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac
     1741 gacaaaaacg attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga
     1801 taatttgtgt tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg
     1861 ctcgttcatc actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa
     1921 agaaaatatt caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa
     1981 ataataataa aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat
     2041 tccaatattt ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata
     2101 caatcttggt ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat
     2161 aacacctatc tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta
     2221 tcctgtataa agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt
     2281 tcatgagtgt ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat
     2341 cgttattgat cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt
     2401 gtttcgataa ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat
     2461 ccaagtaggc aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc
     2521 aatatttcaa aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg
     2581 catatcctac gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg
     2641 tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga
     2701 tttcaatggc tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt
     2761 ctggaccagt cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct
     2821 caggagacgc aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta
     2881 acatccagtt atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt
     2941 tactactatc agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt
     3001 gttcagtatc taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt
     3061 gtacctgcat ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt
     3121 ctttaaaatc agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg
     3181 ctccgttttc tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt
     3241 ttttagaaga aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca
     3301 tttcaatttt gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata
     3361 tttcaccatc agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat
     3421 cagattctgc tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca
     3481 aagagccaat taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga
     3541 catctgacca cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct
     3601 tttacatttg ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt
     3661 gtatcaaatc cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag
     3721 tgaacagtta gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg
     3781 tcatttatta cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag
     3841 tttgctgaac gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata
     3901 aatggataat agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca
     3961 ataacgttta ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa
     4021 gtgattaata gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa
     4081 ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata
     4141 ttccattcat caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta
     4201 tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa
     4261 tgagaaagat catttttttg gttgccataa tattgatgca gagacaaata atctatttga
     4321 tcgtacgctt gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc
     4381 gaacttccgc ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt
     4441 tcctctgcta accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc
     4501 atttcattcc ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc
     4561 cttaaatcac tccaataagt tcctttagga aaattacaat attcgactaa attacgagct
     4621 gcatctactc cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga
     4681 caccagttat aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa
     4741 tccaaccttc taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac
     4801 acaaaatttc caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg
     4861 acgtcttggc gaaatccact tttattagct gatggatgat ctggctgata aatcccttca
     4921 tatactgctc gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct
     4981 acacgcttga cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata
     5041 catgaataat acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt
     5101 ttttatcgat tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa
     5161 cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca
     5221 actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat
     5281 caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact
     5341 tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat
     5401 caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat
     5461 tagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg
     5521 tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat
     5581 cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct
     5641 ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac
     5701 attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa
     5761 ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc
     5821 tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa
     5881 tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga
     5941 acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg
     6001 aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt
     6061 cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct
     6121 attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt
     6181 atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc
     6241 accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat
     6301 caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt
     6361 caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca
     6421 attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac
     6481 caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc
     6541 ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc
     6601 ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa
     6661 cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt
     6721 ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt
     6781 tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta
     6841 aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt
     6901 tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg
     6961 gtttgacacc taattcattg ataatttctg tctgcaggct catttattct cctcctatgc
     7021 ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt
     7081 ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc
     7141 ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa
     7201 ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc
     7261 tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc
     7321 tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg
     7381 agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa
     7441 cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc
     7501 accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat
     7561 tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata
     7621 gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata
     7681 ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt
     7741 cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa
     7801 cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga
     7861 ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg
     7921 tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc
     7981 atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga
     8041 tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc
     8101 tgtttctact aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc
     8161 aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa
     8221 atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc
     8281 attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta
     8341 tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg
     8401 acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa
     8461 actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc
     8521 ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag
     8581 cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc
     8641 ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac
     8701 gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca
     8761 atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa
     8821 tcaatggcaa aagtttttga agcaataata cgtctttc
//
LOCUS       DAFGFZ010000112         8835 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000112 DAFGFZ010000000
VERSION     DAFGFZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.114"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(234..2615)
                     /locus_tag="LR726_002385"
     CDS             complement(234..2615)
                     /locus_tag="LR726_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL6217429.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2605..3252)
                     /gene="recU"
                     /locus_tag="LR726_002386"
     CDS             complement(2605..3252)
                     /gene="recU"
                     /locus_tag="LR726_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL6217430.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3316..3855
                     /locus_tag="LR726_002387"
     CDS             3316..3855
                     /locus_tag="LR726_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL6217431.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3945..4364
                     /gene="gpsB"
                     /locus_tag="LR726_002388"
     CDS             3945..4364
                     /gene="gpsB"
                     /locus_tag="LR726_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL6217432.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4386..4753
                     /gene="rnpB"
                     /locus_tag="LR726_002389"
     ncRNA           4386..4753
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LR726_002389"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4962..6128
                     /locus_tag="LR726_002390"
     CDS             4962..6128
                     /locus_tag="LR726_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL6217433.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6174..7670
                     /locus_tag="LR726_002391"
     CDS             6174..7670
                     /locus_tag="LR726_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL6217434.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7751..8434
                     /locus_tag="LR726_002392"
     CDS             7751..8434
                     /locus_tag="LR726_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL6217435.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2790 a         1589 c         1867 g         2589 t
ORIGIN      
        1 tagtgtgtat ttagccgatt taattataaa caagtaaatg gataaagtat atgtaatgat
       61 aaaagaaaaa tggattttct aatttttaag ctaaatgtta gatcacacat gtatatgctg
      121 gatagtctta taaaaaatca tgtaatacag cttggtacat gatttttatt tgtagctgtt
      181 ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt ggtttctttt ttactatctg
      241 tttccagaag ctgaggtgct gctggatggt gtatggtcat ttgcagcatc cgctccgcta
      301 gttgctggct cactggaaga agcaggagga gcagatgaag acggtgtcga gctttcttcg
      361 gatgaagaag aaggaaccgt tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt
      421 tcttttgagg actcagcagt tgattcgctt tgactagtag agctagagct ttgagtcgtt
      481 gtctctgttg ttgagcttcc aggagtctgg acatagctgg aagatggaat agttgttgat
      541 ggtgtaattg cattcgatct agctgtatat tgatctttgt agtacagttc accgcccaca
      601 cgaattaatc cgcttggcat ctcccagtct gtatttgtca cgttagctga aacatactgc
      661 ataagctcgc gataaacatc agaggctacg tgtgacgatt ctgaagtgac aggtgtcatt
      721 tttttattgt aacctgtcca cacggaaatc gaataatttg gcgtatagcc cgcaaatagg
      781 atatctggat acaccccaga agaaatacca agttttgcat actcgtcatc tgtgtaattg
      841 gatgttccgg tcttaccggc ttgatataac ccagcgattt gcgcattcgt accagttcct
      901 tcagtgatcg tatcttttaa aatgtctgtg atcatataag ctgtttctgg agacatggct
      961 gtttttccat ctggctcata agtttcttct gttccgtctt ggaagacgat tttattaaca
     1021 tattgaggtt tgtaataagt ccctccattg gcgaaagccg cataggctgc tgccattttt
     1081 aaggaagaag ctccgtattt tgtcccatct tgttcttccg tattactaga gatcgcattc
     1141 gattgatgga tcgtactgta ttcgatccca agatttttta agaaagaagc cactttatct
     1201 gaaccgactt cattgaataa tttgactgct ggaacattac gggataagta aagtgcttgg
     1261 cgtaaagtaa tggtgcccat atattgatta tcccagtttc ctacaggagt cgaagtccct
     1321 tcatagttat acggtgcgtc agtaatagtt tttccagtag aatatttcaa atattcaaaa
     1381 gctggtccgt aatctgtgac aggtttcatc gtagaaccaa aatcacggga agtattcact
     1441 gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac gtccgccaat ctgggctttt
     1501 actttcccgg tattggtgtc gataagggtg gaggcaactt gcatctcgtc gtctgggtaa
     1561 gagacatact gatccgtgtt gacgatatca tatagtttct tttgggcgtc cagatccaaa
     1621 ttcgtataaa tctctaaacc gtctgtgtag acattcttat ctgttttttc ttgtacttca
     1681 ttgatgactt ctttgacata attatcgacg atcttagtat tgtcatcgga ttgagttagt
     1741 tcttgcagac catccgttac cggtacatta acagcttgat catattctgt ttgagagatt
     1801 ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga
     1861 taaacataag gatcataggc ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc
     1921 ggtaaagaaa gttctgataa tttcttacca aagtacatct cagaagctgt ctccatcccg
     1981 tacaagccgt ttgacatata cactttattt acgtagtagg tcaagatctc ttgtttcgat
     2041 tttttttgtt ccaatcttac agccatccac gcttcttggg ccttgcgttt cagagtctga
     2101 tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg
     2161 ccttgcagtc cgccagaggt aaagttagag agggcagaac cgatgatccg aatagggtca
     2221 accccgatgt gtttataaaa acggcgatct tctaccgaca cgatcgcatc ttctaatgtt
     2281 ttcggtaatt cattcgcaga aattttttca cgcttttctg cgcctaaatc ttcgaatagt
     2341 tcgccatctt gtgtatatag tttggaagat acggtagcgt ctaatttttt atccgtcaat
     2401 tcgggcgcat cttttgcgta ataccagaaa agtcctacac ctgctaaaaa gaggatacag
     2461 aaaaatgaga gtattcctaa aaggatcttg ataaataatc cttttttatg tgttccagag
     2521 gactttccag aacctttgcc agaattcttc ttcacactct ttttaggagt aggctgtttc
     2581 tgtcgtctag agcttctagt ttgttcattt gccattcttg ctttctcctt caatagataa
     2641 tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt
     2701 gatctctatt cctagttctt cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt
     2761 ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa taaaaacagc gcttcaaaga
     2821 cgaaaaccat aacaagacaa agcagacccc attttgttcg aggcatgctt tcatatgttc
     2881 gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc
     2941 ttcaaagtcc aagtaaaagc ctcgatagac gccattatag tctgtagttg aggcttgggt
     3001 aaagtaggct tccttgatca cagcagcact tctcttagga tagtctactt tcacgatttg
     3061 gataggggta ggctttttat ggataaccgc caacccgcgg gaaagataat atttattact
     3121 ttcattgatt gcttcttcaa agcgcatccc ccgattgccg aattcagtga tctgatttct
     3181 tttttcttga attttttcga cgggagaggc attcttataa taaggttggc catttggata
     3241 gcgcaatacc atctttaatc acactcctag gccacattat agcaaaaaag cggatagaaa
     3301 gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc
     3361 gaactcggtg tttttaaaga aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa
     3421 aaagaactcc atcagttagc agaagaaggg ctggaatggg tacttgtcag tgggaatttg
     3481 ggcgtcgaac tatgggctgt gcaaatagta gcggaattga aagaagaata tccggaacta
     3541 tctcttggta ttttgtatcc tttttctgat tttggtaata actggaatga agggaaccaa
     3601 gaaaaaaaac aattagcaga acaattagca gattatgtcg aagctgttag ccatcaaccc
     3661 taccaatcac ctagtcagct aaagaatcac actcgtttta tacttgagca tacagtgggc
     3721 tgtctgctga tctatgatga agaattccct ggaaaaacga agtatttttt tgaagatgca
     3781 cagaattacc aagcagatca tccctacgaa attcggctga tcagtatgga cgatcttcaa
     3841 aattttagtg aataatcgat attgtgtttg atttatcaac ttttttccta tacaatgaaa
     3901 aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt aaatatggcg aatttggttt
     3961 atagtcctaa agacatttta caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg
     4021 ttgaagttga tgaattctta gacaatatca tcaaagatta tgaaacttac agcaaagaac
     4081 tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa
     4141 ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc accaaagagc gcagcagtga
     4201 ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc
     4261 ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa tccaaataac tacacaaatg
     4321 cagacacaag cttagatgat aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat
     4381 ttgcggtttt cgggtaatcg cggtctgact tgatcaggct gaggaaagtc catgctcgca
     4441 caggctgtga tgcctgtagt gttcgtgctt agcgaaacca taagctaagg tactctttgg
     4501 agtaacggca ggaaaacaag ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca
     4561 cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc
     4621 aacccaaaca atggtagggg cgctttttgt ctggaagaag aacaggacaa aaaggcagta
     4681 taactgcaga tagatggtta ccgctttgtt ttttctccct ggaaaagcaa agtacagaac
     4741 atggcttata gaaaaccgca aacatcaggg aattaggtcg tgacaaaagt aatgcgttat
     4801 aagaaacaag aaaaacattt gaaagctatt tttcaatact tcagatgaat cgttcttatt
     4861 acaaaacaaa cttttgtatc accggttatt cggaatttga gtaaaagtgt ggggtaggac
     4921 tgatgtccca cctccttttt ttataaataa ggagattgtg tatggaaaaa aacaggaagt
     4981 tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact ggttggtaaa gaattaagag
     5041 atcttggaat cgaatgccaa gttgagaacg gacgtgcacg ttttgaagga acaatggaaa
     5101 cgatcgctac agctaattta tggttacgta cagcagatcg agtaaaaatc gtggttgggg
     5161 aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag
     5221 attttcttcc actagatgca gcttttcctg tagctggaaa atcgatcaag tcgaaactat
     5281 atagcacacc tgattgccaa gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct
     5341 attatcaccg tccagcttct gtaccactga cggaaactgg cgcacatttt cagttggaag
     5401 tagctttgct gaaagaccat gtgatggtaa ctctggatac gacaggacca agtttattca
     5461 aacgtggtta tcgcttagaa aaaggtggag caccattgaa agaaaacatg gcagcagcac
     5521 ttattatgtt gacaaattgg cgaaaagacc gtccatttta tgatcctgta tgtggttcag
     5581 gtacgctttg tattgaagca gccttaatag gccataatat cgcaccagga ttcaatcgtg
     5641 agtttgcttg cgaatcttgg gattggttca gtcaagaaat catggaaaat gttcgatcag
     5701 cagccgaaga aaaagcagat tatgatattg aattagatat tactggttca gatatcaatg
     5761 gacggatgat tgaaattgcc aaagccaatg cagaagaaat cggattaggc tcttcgatta
     5821 cctttaagca acaagcagtc aaagacttca aaacggataa agaatacggg gtcatcgtgg
     5881 ctaatccgcc ttacggagaa cgtctaggag aagaagaaac tgttcggaaa ttatataaag
     5941 aaatgggcga tgtattccgt ccattaaaaa catggagtaa atatatcttg actagcgact
     6001 tagcgtttga ggaattttac ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg
     6061 cattgcgtac ggatctattc cagtattggg gaactcgtcc gcctagaaaa aaagagaaag
     6121 acgaataatc ttactaattg attcattcaa ttggatgttt ggaggaatag ggaatgaaag
     6181 aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc
     6241 tcgattggga tacacaaaca ggtatgcctg aaaaagctag tgcataccga agtgaagtag
     6301 acagctatct ttacagcatt tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca
     6361 ttcgctattt ttctgaacat ccggaagaat tgtcggaagt tggaactgcc gtttttgcca
     6421 aagtaaaaga agagtatgat ttggaacatc gcgtaccgga agatcttatg agaaaatata
     6481 cagctgccgt ttcttcagca cacggacaat ggcaaaaagc tagagaaaca caacaatttt
     6541 ctgattttca acacgcattg aatcaaaata ttgaattaac aaaacagttg ataccttact
     6601 ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa tcaatacgaa cccgggatga
     6661 ctgtcgaaat ccttgataag gtatttgatc agcttaaaga aggaattctt tcgatccgcc
     6721 gtacgctcca agaaaaaggg actgaacctg aaacagactt tttaaatcgg agaatgacga
     6781 aaaaacaaca gaagagattt gtagtgaaag taatcgaaca acttggttat gattttagtc
     6841 gtggtcgttt agatgacact gtccacccat ttatgactgg aattaatccg aatgatgcac
     6901 gaatcacgac acgatggaac gaaacagatt tcaaaatggc tgtttttgga gtgatccacg
     6961 aggctggtca tggtatgtat gaacaagaca tcgatgataa atatgcgtat acaccggttt
     7021 atgaaggtac atcaatgggt atccatgaat cacaatcgtt gtttaacgag atcattattg
     7081 gaagtagtcg cagcttctgg aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa
     7141 cattcgatga tattcctttt gacaaatttt atcgttcatt gaaccataca aaatcaagtt
     7201 tggttcgtat cgaggcagac agtttgactt accctctcca tatcatcatt cgttatgaaa
     7261 tcgaaaaaat gattttcaat gaaggggtag atgttgctga actacctgaa atatggaatc
     7321 aaaaatatga agagtatctg ggaatacgac caacaaatga tgtggaaggc atcctacaag
     7381 acgttcattg gtcaggtgca agctttggtt attttccctc ttatgcatta gggtacatgt
     7441 atgcggcaca attacttcat tctttgcagc aagaaataga tgtggaaaat gttctagcgt
     7501 cagacgatta ttctccaatc aaaaactgga tgtcagagca tatccatcaa tatggtgcat
     7561 ctagaaaacc aaatcaatta atcatggatg caacacatga accgcttaat ccgcaatacc
     7621 tgatcgacta tatgaaagac ctgtatttta atgtatatca ggtaaaataa aatacaattt
     7681 acaaagatag ctgaaattca tctaatctat agatgttttc agctatttac ttaagaaaga
     7741 agtggaacga atggataaat tacaagtgat cgctgattat agtatggacc aattagcgca
     7801 tgatcaaaca ggacatggga gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat
     7861 tttggagacg gaaccgcagg cagaccgctt tgtgacgctt gcagcagctt acctacatga
     7921 tacgattgat gataaagtcg ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt
     7981 ccttcgctct ttgccaatag tagaggaaca gatctcgatg attttttcca ttatcgaaaa
     8041 tatgtccttt tctaaaaatc tctctgaagc ggccgaacta tctctggaag gtaagatcgt
     8101 tcaagacgct gatcgtatcg aagcattagg agcaatcggc attttgcgta ctgcttattt
     8161 cggcggtgga catgggcacc cgatttttga tcctgaactg tatccgcaaa cattcaagga
     8221 caaagaaaat taccgaaaag gcacgacagt gatcaatcat ttttatgaaa aactgttctt
     8281 gctgtctgat aaaatgaata ctgattacgg atatgaagaa gccaaacgaa gagaaacctt
     8341 catgcgtgaa ttcttagaag aattttttct ggaatggtct attgacgatg aaaattttac
     8401 tccagacaat tggatcaaaa ataacaaaaa atgatggagg ttgtgacaga agtggtcagc
     8461 tccaagatat ttcggatcat tttccgtaaa agttacttct ggaacactgt ttaccaagag
     8521 gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg
     8581 tgtttgatga atattgtaac ttgtgattcg gaattggcag gagaacaccg tttattcgga
     8641 agaataggac tgtgacaaga cttttgtcac agtccttttt tcgaattcaa caagtaaaac
     8701 ttttaagaaa tattcagaga gtaaaattaa aaattgctga taaacgaaga tggaaatttt
     8761 atgtattttt attggtctat gcaataaata aattaaatat tcagaataca attgatttta
     8821 acgaaagggc ttcta
//
LOCUS       DAFGFZ010000113         8785 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000113 DAFGFZ010000000
VERSION     DAFGFZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.115"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            15..314
                     /locus_tag="LR726_002393"
     CDS             15..314
                     /locus_tag="LR726_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217436.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(411..845)
                     /locus_tag="LR726_002394"
     CDS             complement(411..845)
                     /locus_tag="LR726_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217437.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1077..1787)
                     /locus_tag="LR726_002395"
     CDS             complement(1077..1787)
                     /locus_tag="LR726_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL6217438.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1777..2637)
                     /locus_tag="LR726_002396"
     CDS             complement(1777..2637)
                     /locus_tag="LR726_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL6217439.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2642..3280)
                     /locus_tag="LR726_002397"
     CDS             complement(2642..3280)
                     /locus_tag="LR726_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL6217440.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3295..3594)
                     /locus_tag="LR726_002398"
     CDS             complement(3295..3594)
                     /locus_tag="LR726_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217441.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3637..5121)
                     /locus_tag="LR726_002399"
     CDS             complement(3637..5121)
                     /locus_tag="LR726_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL6217442.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5141..5602)
                     /locus_tag="LR726_002400"
     CDS             complement(5141..5602)
                     /locus_tag="LR726_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6217443.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5620..6687)
                     /gene="ulaG"
                     /locus_tag="LR726_002401"
     CDS             complement(5620..6687)
                     /gene="ulaG"
                     /locus_tag="LR726_002401"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL6217444.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6905..7675
                     /locus_tag="LR726_002402"
     CDS             6905..7675
                     /locus_tag="LR726_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL6217445.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7948..8775)
                     /locus_tag="LR726_002403"
     CDS             complement(7948..8775)
                     /locus_tag="LR726_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217446.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2661 a         1850 c         1385 g         2889 t
ORIGIN      
        1 aaaggaaata aaacatggat tatactgttg aatttgacag tgaactactt aagcaatttc
       61 cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca gagtatttgg
      121 agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga atatacgaga
      181 tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg
      241 tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa gaggtgagta
      301 aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc tatcctcttg
      361 tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg ttatttgtca
      421 ttaccaaaaa gcaccttttt tcttctagta tctatgacat aagaaatacc acccatttcc
      481 tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt atcatctgtc
      541 aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc attcaaaagc
      601 actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg aataaatgcg
      661 atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc cccattacgc
      721 tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa
      781 ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag ggaatcaata
      841 ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt ctttgactat
      901 ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa attcctcttt
      961 ccttttagaa tgcatacttt ttgataaata aaaaggttgt gataaatttt cttcacaacc
     1021 tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta gcatctctat
     1081 tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag taaatattgc
     1141 gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag acaaatttca
     1201 tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt gaacggtcca
     1261 tgtccataga caagtactcc agggacttcg tttggatcta attttctttc cttaaacgtc
     1321 tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc tttcgttaat
     1381 tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc ataagctggc
     1441 aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg agtaatagca
     1501 gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga agatggtttt
     1561 aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg atctgcttgc
     1621 atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc acggtccact
     1681 tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc cagatttgct
     1741 tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat agccagcctc
     1801 cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa agtcttggct
     1861 tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt tcaaatgtct
     1921 taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa actggcctga
     1981 atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat aatcgatccc
     2041 tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc ctaggtctgg
     2101 atatacttgt agaaaaggtg aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga
     2161 gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt tagaagcttc
     2221 ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg atttctcttc
     2281 ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat gcgccaaatg
     2341 gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag cagaaccaaa
     2401 cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg agctgaacat
     2461 ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta accgctcatc
     2521 cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa gggaaaaacg
     2581 ttctttccat gaaatatctt tgggtaacgc tttttcataa atcccaattt gagccatttc
     2641 cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc atcggatcat
     2701 ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct tcaaataagt
     2761 gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt tcaatcagtt
     2821 ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc gcatcacggc
     2881 tttgatggta gattacctgc cggataccga tgttatgcca atccatggct tgttcaaaag
     2941 tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct gcctccatag
     3001 ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt gcaacattac
     3061 tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt ttatctggga
     3121 acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga attgtgccca
     3181 cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca tgttctaagt
     3241 cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct tcctttactt
     3301 gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttga ctgacatcac
     3361 atttttgata ccgataactg taactccttt tttctctgcc tgagtaaaca tattcaaaaa
     3421 attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct ctgaaatggc
     3481 acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt ccacacttct
     3541 catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc tcatcttaat
     3601 ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct tttctgcata
     3661 tgtgtcatag tcttctacaa tcaaaaaata accatcagga tttttccgat attgtaactg
     3721 cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta ggactttcat
     3781 gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat aaccaccgta
     3841 tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta tgattcctga
     3901 aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat aaactgctat
     3961 aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg gtgatttcaa
     4021 taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt
     4081 taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg gaacagctcc
     4141 tggtaaaatc gtgtttgaga ttccttggaa agattgagtt agttcatcga cgaatgtccg
     4201 aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg tcataatgta
     4261 gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga atcaaataat ctttgcctag
     4321 tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa ccatattctc
     4381 attgaaaatc gacaagaatc ctggtaattt gatatcttca atttttttgt cttgcttgat
     4441 tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg caaatataaa
     4501 gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca attcctgcgt
     4561 gattcctact gttaagttag aagctactgt ccaataacaa cctaaaatca gtcccataaa
     4621 aatcaatgct tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt
     4681 agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt
     4741 ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa tcatcacatc
     4801 tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt gtccaaaata
     4861 aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca agattggccg
     4921 aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga ccgttgcctt
     4981 taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc ctaataacac
     5041 gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat acgtccaaat
     5101 gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat tctgccatca
     5161 caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat ccatctgtca
     5221 ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct tttgctgcga
     5281 gtgtaaaaaa caaactagcc gttttctcct cattctcccc ttcactttca aaacggattg
     5341 gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt ttttctgaag
     5401 aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt tctacacaat
     5461 ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat acaagacagc
     5521 tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga atcaattgat
     5581 ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg acggaaacgg
     5641 tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa actcaagatc
     5701 atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag gtttaaatcc
     5761 atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg gatcagccat
     5821 aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca atgactccgc
     5881 catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt tttcaccata
     5941 ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata aattggagta
     6001 atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat agtttactgc
     6061 aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat cttcacaagt
     6121 gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt tgattttgac
     6181 tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt tttgccacgt
     6241 gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat gaactgctgc
     6301 ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag cgtctacatt
     6361 ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct gcatattttg
     6421 aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc catgactacg
     6481 ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac ttttcagcca
     6541 aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta cttctgtttc
     6601 gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc acttttcttt
     6661 tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt gttccaactt
     6721 acaagtaaat attaatgtgt atcagcatcc atgaaaaccc ttttatatga ccgtttgttt
     6781 tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga cagatatgaa
     6841 tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc aggaggcaaa
     6901 agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt tggaactgct
     6961 tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg tatctattag
     7021 tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac gcaattacgg
     7081 ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc ctgaaaggat
     7141 taaacaagcg attgccagag aagctagtaa atacattaac gattatgata cgctgtttat
     7201 caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc atttgacagt
     7261 cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta gttatatttt
     7321 aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg cattaaatac
     7381 gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg atgtagtaca
     7441 tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg tcaacaagac
     7501 aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat caaagttcaa
     7561 gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt ctgaaaaagt
     7621 attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat tttaagaacc
     7681 aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta tcgataaatg
     7741 aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtatccatc caatcgatac
     7801 catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg aatcaaacaa
     7861 tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa acggtattca
     7921 aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca cctttaccgc
     7981 gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt ctaaataatc
     8041 atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt ccaatgcata
     8101 agtcgattta tgtttcttat tatactcaat acccttctca cataattgga ttgctttatc
     8161 ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc agaccatcag
     8221 attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa
     8281 atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt gtaagtgatg
     8341 gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg ctgcatctgg
     8401 ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat ttaaaagatc
     8461 agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt cttggtattt
     8521 tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt cttcttctgt
     8581 ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac ttaagatatt
     8641 gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt cacaaatgcc
     8701 ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta gtaatgctcc
     8761 atctacttta atcaaaacaa tttcc
//
LOCUS       DAFGFZ010000114         8559 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000114 DAFGFZ010000000
VERSION     DAFGFZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.116"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(942..1355)
                     /locus_tag="LR726_002404"
     CDS             complement(942..1355)
                     /locus_tag="LR726_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL6217447.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="LR726_002405"
     CDS             complement(1811..2296)
                     /locus_tag="LR726_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217448.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="LR726_002406"
     CDS             complement(2435..2650)
                     /locus_tag="LR726_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL6217449.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="LR726_002407"
     CDS             2874..3674
                     /locus_tag="LR726_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL6217450.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="LR726_002408"
     CDS             3693..5561
                     /locus_tag="LR726_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL6217451.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="LR726_002409"
     CDS             5554..6045
                     /locus_tag="LR726_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL6217452.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="LR726_002410"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="LR726_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL6217453.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="LR726_002411"
     CDS             6605..7600
                     /locus_tag="LR726_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL6217454.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7742..>8559)
                     /locus_tag="LR726_002412"
     CDS             complement(7742..>8559)
                     /locus_tag="LR726_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL6217455.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         2971 a         1378 c         1631 g         2579 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga gtttagtcta atggtgataa
     7621 gtcaagatga aatttaagat tttctttatt gagtaatatg ttttgaagcg cacgaaacta
     7681 gacccgatca aaatcaaatt tttttcgggt tgattcaagg atttgcgtca ataacggatt
     7741 gttatttctc tatcacactc cttggtggcg tatagagttg gtgtttacgt agtagcgtat
     7801 ccaccagacg cgtaaatttt cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg
     7861 gaacctcctt atatttactt tgataaaaag ctttgtattc aggaagataa ttttttacag
     7921 agttggcagc ttcaactaag tagtatctaa aatatcgatt tccttgtttt gtcagaggtg
     7981 tattttgcga ctgatagttt ccagattggt tcactttcca acttaatcca gcgtattttg
     8041 ccaattttgt ctgatcttca aatctttcga tttgaccaat ttcagcgatt aaaccggcag
     8101 catagacttt accaacgcct ggaatactgg ttaagcattg atattctgga atgacaacaa
     8161 ttacttgttc gatggcttta tctaattctt tgatattttt ttcaagtgct cgtatttctc
     8221 gtacgagaac acttaaaaca acattaattg attcttgtgc aagagcacct aggcggtaac
     8281 tcatggtaat tgcacgttga attgctttag cgattttttc tggttgcttg aaacggcctt
     8341 ttcctttttc ttgaagtaaa tcacagaaag cttctaaagg aagctcagca agttcatcca
     8401 atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc gtcgattcgt
     8461 ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga aaatgttgct
     8521 ttgtacgaat caattgctta ataagttgat agcgagtac
//
LOCUS       DAFGFZ010000115         8520 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000115 DAFGFZ010000000
VERSION     DAFGFZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.117"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            56..355
                     /locus_tag="LR726_002413"
     CDS             56..355
                     /locus_tag="LR726_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217456.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            620..1495
                     /locus_tag="LR726_002414"
     CDS             620..1495
                     /locus_tag="LR726_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217457.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1492..1710
                     /locus_tag="LR726_002415"
     CDS             1492..1710
                     /locus_tag="LR726_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217458.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1697..4324
                     /locus_tag="LR726_002416"
     CDS             1697..4324
                     /locus_tag="LR726_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217459.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4339..4557
                     /locus_tag="LR726_002417"
     CDS             4339..4557
                     /locus_tag="LR726_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217460.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6076..6390
                     /locus_tag="LR726_002418"
     CDS             6076..6390
                     /locus_tag="LR726_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217461.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6443..7216)
                     /locus_tag="LR726_002419"
     CDS             complement(6443..7216)
                     /locus_tag="LR726_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217462.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7321..7566
                     /locus_tag="LR726_002420"
     CDS             7321..7566
                     /locus_tag="LR726_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217463.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            7722..>8520
                     /locus_tag="LR726_002421"
     CDS             7722..>8520
                     /locus_tag="LR726_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBL6217464.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTK"
BASE COUNT         3290 a         1095 c         1586 g         2549 t
ORIGIN      
        1 gtacttataa aggtagagga gttatagaat aattaattaa aaaggagagg tttttgtgga
       61 agcaataaaa ttaatgattt cagaagaaca ggaggaaatg tttctaaagc agttttatca
      121 aattgctaca aaagctattg aaaaggctca agaaaatgcg gggatgacaa gggaatatct
      181 caatcaaaaa gaaatggcgg aatacttaaa tgtgagtgaa aactttttaa aaaatgctgt
      241 gagaaatgag ggattgcccg tagttgaatt aggggcaaga aaattttatt cgaaaaaggc
      301 agttaatgag tttatgtttg ctcgaatgaa aagaggggaa aatattggaa gatgatgaag
      361 tagaaattat aaaaaaatgc tttaaaaaaa gaaaaaaagt gttcccaaaa gtttgcagac
      421 tgggggaaca ctcaataata aagtgctgag aaaactttat ttatagctat tatatcaaaa
      481 aagaaggtga aaaggtgttt tcttctggaa atgttgtagt tgacaaactt agagaaataa
      541 attttgacgg aaatgtaatc cacacacatg gtacgatcaa ttaagaaaaa aaacgaaaaa
      601 aggtgttgag aagccctatt tgaatgcaat agttatctta gcagaaatta cctattggta
      661 tagacctaaa aaaattttta acgatgatgg tcagctactt ggttatcaaa aaaagtttct
      721 tgaagatatt ttgcaaaaat catataagca attaagcaaa aaaacaggat tatcagaacg
      781 agctataaaa gatgcaattg tgtacttaga gaaaaagggg attatcaaaa gggtttttag
      841 aaatatttat tatgagaaaa gagcagttac attatcaaat gttatgtaca ttgttttaaa
      901 tgtacataaa ttgatagaaa ttacttttcc tactttggat tgcgaaaaaa aggagtatat
      961 gccgaaaatt cgtcagaggg gtatcgaaaa aaagagatat gtctctttaa aaaaaggtag
     1021 aggtatgtac aaatttgtat atacgtatac aaagactact tcacagaaca tatcaaagac
     1081 tactctcgtt atggaagaag aggatattaa tataaagagg atttacagta agattagtgt
     1141 tctttatgac gcaaataacg aagatggtcg aaaattattt caagtgtatc actatttgag
     1201 aaacgctaat gtagatacag aagatatcag aaaaatcatt gaatatttat ctgaaaatac
     1261 tcagtatctt attgctgagt gtattataaa acaacatgaa gcatgctttt ttcaaagaaa
     1321 aactgaagaa gggttatacg attatgtaac atattttttg aatggacttc agaaaagata
     1381 taaaagtcaa tttttagaaa atgtagagcc tatagatcag ttcaaaaaag ctttcgaaat
     1441 caaagagtta cctgaaatta ctatgcataa ttggttgaaa ggagaagaat catgattaaa
     1501 aatcgcttaa tagatcaaat gtggtgtctt gatcaaaata cagatactct cgaattaatc
     1561 gagagtatct gcttcaacac agtagttctc gatttaagag ctgaaaatga caattgtgta
     1621 actcatatca taattaatca aaaaatggct cagcagctct ctgagagcct aagaaaatgg
     1681 gctgaaggag aaaactatga aagcaattaa agaaaaaatc gttagtagaa aaataaactg
     1741 gttagcactc agtgtacttg ttttaatgat tagcgttgtt tttacaagta atattgttga
     1801 agctagcatt ttcaattggt ttgatgacac aaaaagtcaa acagagtttt tgaataattc
     1861 tctttataga ccttacctaa aaaaatacaa tggagatttc cttagtcagt tcgggatttg
     1921 gcttggatgg gctgtggtaa aaggcatgtt tactgtgact gattcgattc aaaatatgat
     1981 tccagatgtg ttggatttgt tcaatttcat tgaaagtaca gggctaaata atgtttatca
     2041 atcagtcata aatacaattg tcgttggatt aatgatcctt tcattgatgt ttgttggcta
     2101 caagatgatt actggaaaag gaacaataga tttaagatct gttggaatga atatagtgat
     2161 gtctgtagcg ttaattcttt taatgccaac aatgatttct agcggtattc aatttgccaa
     2221 aatattttac aatgatgcta ctacgattac aaattcagat gacggagtcg cttggtcatt
     2281 gataaaacaa ggagtaacag atctggcata tattaataaa acagatcaat actcaagcat
     2341 tgataaaacg gaggatcgaa ataagttaac acgaaaaaat tttcaacaga cagatttaac
     2401 acaagtgttg acggataaag tgattgataa attggaaaaa gaaaatccag ctgcggataa
     2461 tcttcgctat gaattagttg aaaattctaa taatgaattt gttgccacaa agttttctga
     2521 taacttctta tcaacatttt ctgatagctt gaaatcaggt tattatcgat atcaagcaaa
     2581 tctttggggg atttcgattg ggttgacagc gttggcaatc gcctatgtgt tcagcgcgtt
     2641 tgtgatcatt acagcaatat tagaacttgc atttaaacgt gtgttaggtg ttcttgtttt
     2701 tgcgacagat attgaaacag gacaacgttc aaaagttgtt ttatccgata ttttacaatg
     2761 ttacttaaca gttggatttc aagggtttgg actatctatg tttgctatgt ttatcaattt
     2821 tttaaatgct ggacaaggta tctccacaaa tatttttata aaaacaattg cttatatctg
     2881 tgctgtgttt gtgctgataa aaggcagtgg aacagtcatg agatactttg gtgttgatat
     2941 tggactcaaa gaaggctatg ggcaacttgc ttctgctttt ggtatgggcg caatgctgtt
     3001 tagaaaagga tcaactggat ttaatcgtgc taaaggaagc ggaaatggaa atggatcaga
     3061 cagtggagaa ggagaagatc gaaaaccaga aaagaatttc ggcgaaactc tatcaaaaaa
     3121 agctggcaaa actggaagag ctttgggata tgcccatgaa cgtggattat caggtttagc
     3181 ttcagatgga gctacaatgg ctagtgaaag agcaataaaa ccatttaaga gtatgcgtga
     3241 tatggctaat gataccaaga ataagttcaa agaaggttta gatgatggta cagtgtccgc
     3301 tattaataaa aatagcaaac ctatgttagc taaaaataaa gaagatgaaa ctggaaaata
     3361 tgctgattcc atgccaacac gtctttcgga tagaaaagat ggtgctaagg ttgaaaatgc
     3421 cgatagaatt atgtctagtt ctgaacgtat gcgtgaagca atgaaaaata atgctgaatc
     3481 gaataataat cctgtaagtg caattcagca aaaagttcaa caagatattg aagaacgtaa
     3541 aaatgctatg cacggtcaag ctaagtctgc tgaagaatta attaatcaga agcgacaaga
     3601 agctaaatac acgcctgaag caatgaatcg tgaggaaatg ttaaggaaac gtgtagaagg
     3661 gcgtacagga gctaatacgg acgaaaaaga agcgttgacc aaagaaagaa tccaagaagc
     3721 taaaaactca aataccggat tagaaaaatt ggtcaaagaa aatctacaaa attctacaag
     3781 tagtcaaggt ggccgatcag tagatgtccg agaaaatatt caaggttcat tgaatggtca
     3841 tgtcggacgg aatgttgatg taagagagaa tttacaaagc tcttcaaatg gtcaaggtac
     3901 taagacaatt gatgttcgag aaaatgtaca aaattctaca agtagtcaag ggtttaaatc
     3961 aatggatgta agagagaatg tacaaaattc tacaagtagt caagggttta aatcaatgga
     4021 tgtaagagag aatgtacaaa gttctacaag tagtcaagga ttcaaaacag tagatgttcg
     4081 ggagaacgtt caaaaagata gtggagaaat gaaagaaaaa acacaaaaaa tcaatatcgt
     4141 tgaagacaga aagtcagcga aaaaatttga ttcaaatcat gaaacagtga ttattgatag
     4201 tgaaatccgt gaaaatgata agggatcaaa accaagacga agatttacct atgaagacaa
     4261 cgagttattc agagatacac ttaatgatcc taatccgctt ttcgataatc tattaaaaaa
     4321 ataaaagggg gaattgtcat gaaattaatt ctaaagatta gtacgcatga tttcttagaa
     4381 cttgacgaat tatgtgaatc agtaaagaac gcaacaaatg aaaaaatcac ggtcactaat
     4441 tatattgaac tgatgattca agaagaatta aagatgaaac atgagataaa aaacaacttg
     4501 aaatattgtt atctagaaaa aagtgaagag ataatccatg aattaaatgg cttttaaaaa
     4561 ttgtatttga catgaattta cgacaaggaa ttagtgggga ttaagcgatt attctcttcc
     4621 actttagaga aataatgggt taaaaagcca gaatacgtag agcaatattg acaacctata
     4681 aaggagctat tgttgaaaat tgaagtaaca aaaagataaa gagaggttta catctatcag
     4741 attaaaagtg atagtacacc catgaaacct gcaaaagagt tgaacctaat gtatatcggt
     4801 ttaactcttt ttatttgcaa attaaaaaaa tatcaccgaa gtgacgaatg aaactatgac
     4861 caaattaagc aagaaaatat atcttgcaaa taaagtagta taaaaagata ttgataaagc
     4921 caatcaaaac tttgaatttc aaacgttaga taatcaaaca gatacaaaaa atgaaatcag
     4981 ttctacgcgt acgattatta aaaataataa attttcacta gaagaaaatg gaaataaaat
     5041 caaataatta ggaagaaaaa acaaagcaat tagaaaagcc tgcaagcaaa tttgagtaat
     5101 cgagatccaa tttttttaag gaagtttaac aaaaatatgt aattctatta tcactattaa
     5161 agagaaagtt ggagcggaat ataaagaaaa gagaaaagta attaaacgca aaaaaatgta
     5221 gaacttaaaa aatataaaga ggaacaaccg ttaaaattag gaatgatgtt gtgtaaacat
     5281 tataattgct atgatataaa tgaagtgtta aagaaagcaa atttaaacca attttgtgaa
     5341 cagaaaagcc ttagtcacta tgaccaaaac tacggacgaa actatgacca aaagtaggta
     5401 ctaaattatg accgaaaata ggtccaaaat ggattttttt atgatatcaa aaattatgaa
     5461 atgattttga aagaaaacca gttggcacag ccaacttagc caccttcctg taggaaggtg
     5521 ctatcagttt tcccttatgc tcttttcttt cttttttgtt tcatatgtca aatgccattt
     5581 tgaaatgtac catcttaaga aatttatcct taaaaacaat tgcgttattg gataaaaaga
     5641 tgctacatta tcttaaaaaa ggaatcaaat atgatgaatt tagtggctag catatcgtgt
     5701 gattaagata ctttatagtg taagttgaag gagaaaaaat atttaaaata tacttcccat
     5761 ttcagtggga agtaaaatta atcttgttgt ttctactagt aatgtatctt tttggaaact
     5821 atgtaacgat gtaaacaaga tttttttcag aagaaaaaac gataaaagtt gtgataaacg
     5881 atttaaaaaa taggagaaaa tatatcaagc gcatggtatg aaacgcacat ttaaaaagat
     5941 tcgactaact aaaaaaataa gtttacaaag cattgttgtg tattacaagt gatctataca
     6001 ctgaaaaatg atagagaaaa tctgatacgt ccgcaacaat ggacaattga aaatgaaagt
     6061 gaggaaattg gaagaatggc tgatgaaaat aaagttgttc cgcttgaaac actagtgata
     6121 aataaggaag agtttgagca actttttctt gatttttttg ttcaatatga caggatttat
     6181 gattttagtt cgtcactaga tttatttgaa ttagaggcag aaataataga aaacagtcga
     6241 gaggagaatg gtaaaatcat catttcagta gaaaaattca atgaattatt ctcagaaaca
     6301 tttgaaatag atacagatta ttatcaacca caagaagttt tccaatcatt tagaaatcag
     6361 attattcgct caggtaagac cgtaaactga aaaacaataa aagaaacgtt gaaagaataa
     6421 ttattccttc aacgtttctt ttttagaatg ttttaatgac caaagcagtt tcattgtcag
     6481 gcttaggtaa ttcatgaaat cccgtattta tagtgctcaa ttgatcaact aaccatgttt
     6541 tactttttga attgtagata aatctaaaat taatatcgtt attatcgtgt tgaaggggcg
     6601 tctccaaata atctgctgta tattcatagt caacgtaacc tgctaataca aaagagagta
     6661 tccctttatt taatgttaga tttaatggat aatcatacga ctttattttt ttcaacttca
     6721 tattgtacat atcttcagcc ataaaagttg agttcgtgat ttcatcttgg gtctcattaa
     6781 aaaagttatc cgaataatcg cttatttttg aaaagtcttt attggcacca gcctcaaaaa
     6841 attgttcaac tttttcagta taaagttgtg ctaacgctcc tttttcaata ttaactttaa
     6901 tattttcatg tctatcaatt tgtgtgctgt tcagtaaggg aagatcagct tttagtttta
     6961 tatcgtcttg atttctttca atttcaattg gcaaaatctt ttcagtagat tcagtttctg
     7021 ataaaggaac atctagtttt ttatcatgat aataagcttc aaatccatct ggaaccggaa
     7081 aaaaatgtat tgggtaattc ttttctgagg ttgttgtact agtttccgta cttgtagaac
     7141 ttgtgtcaga agtggtacac ccgcttatag ttccgatgag taaagcgcaa gttagaatga
     7201 ttgcaaattt cttcaaaaaa attcctcctt aaaattgaat atggactaat actagtccca
     7261 ttgtagcata ccgaatcttt aagattaata agaaaaagga ctaattttag tccaaaaggt
     7321 gtgctattct tgaaagagga aaaaattatt gataaagaaa atgtaatcgg tagcaatatt
     7381 agacgagtcc gattagaaaa aggaattggt caaaccgagc tgattagaga cctacagctt
     7441 cgtaaaattg ctataactag ggaaacgttg gttaagattg aaggtggccg ccaacacatt
     7501 aagttggatc aattgaaagc catcaaagat attctacagg tgtcttatga agatttgctt
     7561 caatgactac ttgtagtcat ttcgtttgaa attattatgt tacaccctta tgtgtcacga
     7621 agaaatactg gtataataat gaatgggata tgtaacgata gggtatactg ttatgttgcc
     7681 taataaattg tggatcaaaa tacaagaatg gagggatatc cttgtataag gcatatgaat
     7741 tggattttac aaatgctgac ttaaatgcat tctcctcaag ctcgcaatca tatacacatg
     7801 tagtaaatca aatagatcac aaccaaaaat ctattaacaa gagaatagaa aatctatttg
     7861 aaagtgaaaa tgatttagtt gattattatt ctaaggacag caaaattcta gatgcagacg
     7921 aaattattaa agattggttt ccaactataa aagcagatat ttttatatct cactctcatg
     7981 ctgatgaaaa attagctata cgttttgcat catggctatt tgaaaatttt ggtcttactg
     8041 catttattga ttcttcggtt tggggataca gttctgattt attaaaaaaa atagaccaaa
     8101 aatattgcta taaggaacaa acaaaaacat atgattatga taaaagaaat gtaacaacta
     8161 gtcatgttca tatgatgctt tctacagctt taaataatat gattgatagt acagaatgtc
     8221 tgttttttct taatacacct aattcaatta gtttgagcaa tgaaatcact aatgaacaga
     8281 aatttacgta ttcgccatgg ctatatagcg aactgacgac agcttcaata gtggaaaaaa
     8341 agaatccacg attggaaagt aatcctcaaa tgagcaccga ggatgttaga agcattataa
     8401 agcactattc ttcgcaagaa aatctgcaaa taaaatattc tttaaattta agtgaattaa
     8461 cttatttacg aaaagctgat ttgaaaaaat gggctttaga tgctgtggaa tgcactaaag
//
LOCUS       DAFGFZ010000116         8494 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000116 DAFGFZ010000000
VERSION     DAFGFZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.118"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..173)
                     /locus_tag="LR726_002422"
     CDS             complement(<1..173)
                     /locus_tag="LR726_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217465.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LR726_002423"
     CDS             complement(593..1714)
                     /locus_tag="LR726_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBL6217466.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LR726_002424"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LR726_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL6217467.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LR726_002425"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LR726_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL6217468.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LR726_002426"
     CDS             complement(4304..5215)
                     /locus_tag="LR726_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL6217469.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LR726_002427"
     CDS             complement(5232..6239)
                     /locus_tag="LR726_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL6217470.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LR726_002428"
     CDS             complement(6236..8365)
                     /locus_tag="LR726_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL6217471.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="LR726_002429"
     CDS             complement(8370..>8494)
                     /locus_tag="LR726_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6217472.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAFGFZ010000117         8490 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000117 DAFGFZ010000000
VERSION     DAFGFZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.119"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            414..1853
                     /locus_tag="LR726_002430"
     CDS             414..1853
                     /locus_tag="LR726_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217473.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2148..2366
                     /locus_tag="LR726_002431"
                     /pseudo
     CDS             2148..2366
                     /locus_tag="LR726_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2615..2830
                     /locus_tag="LR726_002432"
     CDS             2615..2830
                     /locus_tag="LR726_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217474.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3047..3303)
                     /locus_tag="LR726_002433"
                     /pseudo
     CDS             complement(3047..3303)
                     /locus_tag="LR726_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3490..4401)
                     /locus_tag="LR726_002434"
     CDS             complement(3490..4401)
                     /locus_tag="LR726_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL6217475.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4420..5424)
                     /locus_tag="LR726_002435"
     CDS             complement(4420..5424)
                     /locus_tag="LR726_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL6217476.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5421..7544)
                     /locus_tag="LR726_002436"
     CDS             complement(5421..7544)
                     /locus_tag="LR726_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL6217477.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7549..8469)
                     /locus_tag="LR726_002437"
     CDS             complement(7549..8469)
                     /locus_tag="LR726_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6217478.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2441 a         1725 c         1563 g         2761 t
ORIGIN      
        1 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
       61 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
      121 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
      181 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
      241 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
      301 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
      361 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
      421 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
      481 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
      541 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
      601 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
      661 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
      721 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
      781 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
      841 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
      901 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
      961 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     1021 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     1081 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     1141 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     1201 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     1261 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     1321 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     1381 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     1441 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     1501 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     1561 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     1621 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     1681 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     1741 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     1801 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     1861 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     1921 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     1981 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     2041 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     2101 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     2161 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     2221 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     2281 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     2341 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     2401 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     2461 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     2521 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     2581 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     2641 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     2701 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     2761 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     2821 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     2881 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     2941 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     3001 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     3061 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     3121 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     3181 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     3241 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     3301 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     3361 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     3421 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     3481 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     3541 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     3601 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     3661 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     3721 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     3781 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     3841 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     3901 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     3961 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     4021 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     4081 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     4141 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     4201 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     4261 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     4321 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     4381 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     4441 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     4501 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     4561 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     4621 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     4681 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     4741 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     4801 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     4861 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     4921 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     4981 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     5041 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     5101 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     5161 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     5221 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     5281 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     5341 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     5401 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     5461 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     5521 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     5581 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     5641 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     5701 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     5761 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     5821 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     5881 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     5941 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     6001 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     6061 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     6121 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     6181 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     6241 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     6301 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     6361 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     6421 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     6481 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     6541 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     6601 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     6661 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     6721 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     6781 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     6841 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     6901 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     6961 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     7021 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     7081 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     7141 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     7201 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     7261 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     7321 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     7381 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     7441 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     7501 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     7561 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     7621 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     7681 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
     7741 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
     7801 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
     7861 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
     7921 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
     7981 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
     8041 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
     8101 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
     8161 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
     8221 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
     8281 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
     8341 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
     8401 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
     8461 ttctggcaag gtttctttcc agccaccccg
//
LOCUS       DAFGFZ010000118         8282 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000118 DAFGFZ010000000
VERSION     DAFGFZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.120"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            476..1339
                     /locus_tag="LR726_002438"
     CDS             476..1339
                     /locus_tag="LR726_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL6217479.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LR726_002439"
     CDS             complement(1468..3090)
                     /locus_tag="LR726_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6217480.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LR726_002440"
     CDS             3339..3860
                     /locus_tag="LR726_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL6217481.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LR726_002441"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LR726_002441"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL6217482.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LR726_002442"
     CDS             5941..6759
                     /locus_tag="LR726_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL6217483.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="LR726_002443"
     CDS             complement(6862..7059)
                     /locus_tag="LR726_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217484.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="LR726_002444"
     CDS             7228..7806
                     /locus_tag="LR726_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL6217485.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="LR726_002445"
     CDS             7901..8038
                     /locus_tag="LR726_002445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217486.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2494 a         1486 c         1632 g         2670 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 ac
//
LOCUS       DAFGFZ010000119         8276 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000119 DAFGFZ010000000
VERSION     DAFGFZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.121"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..382
                     /locus_tag="LR726_002446"
     CDS             <1..382
                     /locus_tag="LR726_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HBL6217487.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="LR726_002447"
     CDS             complement(776..1783)
                     /locus_tag="LR726_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217488.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="LR726_002448"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="LR726_002448"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL6217489.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="LR726_002449"
     CDS             3679..4002
                     /locus_tag="LR726_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL6217490.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="LR726_002450"
     CDS             4013..5695
                     /locus_tag="LR726_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL6217491.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6859
                     /locus_tag="LR726_002451"
                     /pseudo
     CDS             5901..6859
                     /locus_tag="LR726_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6869..7105
                     /locus_tag="LR726_002452"
                     /pseudo
     CDS             6869..7105
                     /locus_tag="LR726_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            7320..8072
                     /locus_tag="LR726_002453"
     CDS             7320..8072
                     /locus_tag="LR726_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL6217492.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
BASE COUNT         2686 a         1266 c         1684 g         2640 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga aggcgacacc attgtgggag ttaaacacaa
     6421 gagcgctgtt attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac
     6481 aggcagacga gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa
     6541 acacttgttt aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat
     6601 aagtaactcg aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg
     6661 gctaaatgag cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt
     6721 tagggacatg gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag
     6781 aaaatcatta aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat
     6841 attgtctagc ttaatttgac aaatcagaaa agaaaaaatg tatcaagttt ttgaagaaat
     6901 gggttatcca attaaaacgg tcaccagaaa actagaagta atcaaagcgg atgatacgat
     6961 cagcgatttg ttagatgtgg aagaaggaga tccaatgttc tatttccata cacagggttt
     7021 ttcgctagaa cgtctgcccg ttgagtattc gatttcgaaa tatcgaggag acattaacta
     7081 ctttgttttt gaacttgcac aataacttca gattaaataa aatgatttat taaagataaa
     7141 agtcgaatga acaaggctgt cactatgaca gtcatgctcg ttcggtttta ttttttagaa
     7201 tagcagcttg caaattctga aaaattttgc tttccttcct gcagaaggct tcttaagaaa
     7261 acttgttcta gcacttttct taagaatttg atagtcttaa aaagaaaaga ggcgatgaaa
     7321 tgttaaaaac ggatcgattc caagcgatca tgtcgttatt agaggttaac ggtacagtac
     7381 gagtcagcga tattatggag aagttttcgg tttcggatat gacagtccga cgtgatttgg
     7441 acgaaatgga gaaacaagga ttattagaac gcgtccatgg cggagcgaaa ttgaaggatt
     7501 tttatcggca tgtggagctt tctcatagag aaaaacagat tatgctgcaa gaagaaaagc
     7561 agcggattgt ggaaaaggcg aatgaaatga tcaatgatgg tgatacgata tttcttggac
     7621 caggaactac aatcgagctc atgtcaaaaa tcattcaagg ggattcactg cggattgtta
     7681 caaactgttt gcctgttttt gaagaattaa ataagcgaaa agggaatcga aaaatctatc
     7741 tattaggtgg cgagatgcgg gaaacgactc aatgctttgt tggtgaaatc acgaataaat
     7801 cgctacagga tatgaatttt cataaagcat ttttcagctg taatgcttta aatgaacaga
     7861 aaattatgac ttcgactttt gaagagggcc aaactcaaca gatcgctctg aataattctg
     7921 ttgaacgctt tttattgatt gatcattcga aaattgggaa aagagatttt tcagtatatt
     7981 ataatctgaa tgatgtaaca gtagttataa cggatgatga tgaacaaaag cactatgaaa
     8041 aaatcgaaaa agaagaaaaa tatgttgtgt aaatgagaaa ctgccaaact ctagataatt
     8101 agaacttggt agttttttgg tagccttgaa tttaagcgaa ttatgttatt tttagggtgc
     8161 atcattacta attcctccga atacttgaat acaccatttg aaaaaagata gtcgattttt
     8221 ttgggatgtt atgtttatgt gaaggaaaaa atagcagtat caatcaccgc tttatt
//
LOCUS       DAFGFZ010000120         8265 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000120 DAFGFZ010000000
VERSION     DAFGFZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.122"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            225..650
                     /gene="lacA"
                     /locus_tag="LR726_002454"
     CDS             225..650
                     /gene="lacA"
                     /locus_tag="LR726_002454"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBL6217493.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            667..1182
                     /gene="lacB"
                     /locus_tag="LR726_002455"
     CDS             667..1182
                     /gene="lacB"
                     /locus_tag="LR726_002455"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBL6217494.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1193..2125
                     /gene="lacC"
                     /locus_tag="LR726_002456"
     CDS             1193..2125
                     /gene="lacC"
                     /locus_tag="LR726_002456"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBL6217495.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2128..3108
                     /gene="lacD"
                     /locus_tag="LR726_002457"
     CDS             2128..3108
                     /gene="lacD"
                     /locus_tag="LR726_002457"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL6217496.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3136..3453
                     /locus_tag="LR726_002458"
     CDS             3136..3453
                     /locus_tag="LR726_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL6217497.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3453..5159
                     /locus_tag="LR726_002459"
     CDS             3453..5159
                     /locus_tag="LR726_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6217498.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5301..6707
                     /gene="lacG"
                     /locus_tag="LR726_002460"
     CDS             5301..6707
                     /gene="lacG"
                     /locus_tag="LR726_002460"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL6217499.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6830..7003
                     /locus_tag="LR726_002461"
     CDS             6830..7003
                     /locus_tag="LR726_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HBL6217500.1"
                     /translation="MKLPSLLKCGKWYVLTGKEWICHSDLELSAFEWEFLQSLDVEIR
                     ETIHFEVNYLPFQ"
     gene            7024..7923
                     /locus_tag="LR726_002462"
     CDS             7024..7923
                     /locus_tag="LR726_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL6217501.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2612 a         1406 c         1708 g         2539 t
ORIGIN      
        1 gtgatggatt aaatgaacaa aaatgaacaa taatttaacg gtgttatcta ttttttaaga
       61 aaacaaataa aaaaaacaaa aaataaacaa aaaagtgttg ctttttgttt gaatgattga
      121 tattatataa acaaagaaat gatgaaaacg ttttttcgaa cattttgcaa aatgttttct
      181 acttctacgt agcatttctt tctaaaattt aggaggtggt caaaatggct attgttgttg
      241 gtgcagatct caaaggtacg agattaaaag atgttgtgaa aaattttctt gtagaagaag
      301 gttttgaggt aattgatgtg actaaggacg gacaagattt tgttgatgtt accttggcag
      361 ttgcctctga agtaaataaa gatgagcaaa acttaggtat tgtaattgat gcttatggag
      421 ctggtccatt catggtagca actaaaatta aaggtatggt tgcagcagaa gtttcagatg
      481 aacgctcagc ttatatgaca cgtggacata acaattcacg aatgattacg gttggcgctg
      541 aaatcgtcgg tgatgagctt gctaaaaaca tcgctaaagg tttcgtcaat ggtaaatatg
      601 atggcggacg tcatcaaatc cgagtagata tgcttaacaa aatgtgttaa gaaaaggagt
      661 taaggaatga gaatcgcaat tggatgtgac cacattgtca cagatgaaaa aatggcagta
      721 tcagaattct tgaaatcaaa aggacatgag gtccttgatt tcgggacata tgaccacgca
      781 cgtactcact accctatcta tggtaaaaaa gtcggtgaag cagtagtaag tggtcaagca
      841 gacttgggag tatgtatctg tggtacaggt gtcggcatta acaatgcagt caataaagtt
      901 ccaggtgttc gctcagcgtt ggttcgtgat atgacatcag ctatatatgc aaaagaagag
      961 ttaaatgcga atgttatcgg tttcggtgga aagattactg gtggtcttct tatgaatgac
     1021 atcattgaag ctttcattga agctgagtac aaaccaacag aagaaaataa aaaattgatt
     1081 gcaaaaatcg aacacgttga aacacataat gcacatcaag cagatgaaga tttctttaca
     1141 gaattccttg aaagatggga ccgtggtgac taccacgatt aaggtataac aaatgattct
     1201 gacagtcaca ctaaatcctt cagtagacat ctcttatcct ctagaaacat tgaaaattga
     1261 tacagtgaat agggtgaaag atgttagtaa gacagctggt ggaaaaggct taaatgttac
     1321 tagagtcttg tatgaatctg gtgataaagt tactgctaca ggtttcttag gtggtaaaat
     1381 tggtgaattt attgaaagcg aattagaaca atctcctgtt agtccagctt tttataaaat
     1441 ctcaggaaac actagaaact gtattgctat cttacacgag ggtaaccaaa cagaaatttt
     1501 ggaacaagga ccaacaattt ctcatgaaga agcagaaggc tttcttgacc actatagcaa
     1561 tcttattaaa caatcagaag ttgtgacaat ttcaggaagt ttaccttcag gacttcccaa
     1621 cgattattat gaaaaactta tccaacttgc ttcggatgaa ggagtagcag tcgttttgga
     1681 ttgttcaggt gcacctcttg aaacagttct aaaatcgtcg gctaaaccta ctgccatcaa
     1741 acctaataat gaggaacttt cacagttgct tggaaaagaa gtaacaaaag atattgaaga
     1801 actcaaagac gttcttaaag agtcactttt tagtggtatc gaatggatag tggtctcatt
     1861 aggtcgaaac ggtgcctttg caaaacatgg tgatgttttc tataaggtag atattcctga
     1921 tatcccagtt gttaatccag tcggatcagg ggactcaacg gtagctggta ttgcatcagc
     1981 tttaaatagt aaaaaaagtg acgctgactt attaaaacat gcgatgacat tgggtatgtt
     2041 aaatgctcaa gaaacaatga cagggcatgt gaatatgact aattacgaaa cactaaactc
     2101 acaaattgga gtaaaagagg tataaaaatg gtacttacag aacagaaacg caaatctttg
     2161 gaaaaaattt cagataaaaa cggtgttatc tcagctttgg catttgacca acgtggtgct
     2221 ttgaaacgtt tgatggcaca gtatcaagat acagaaccaa ctgtagctca aatggaagaa
     2281 ctaaaggttt tggttgctga tgaacttaca aaatacgctt cttcaatgtt gcttgaccct
     2341 gaatatggac ttccagcaac aaaagcattg gataaagaag caggtcttct tcttgcctat
     2401 gaaaaaacag gttatgatac atcaagtaca aaacgtttgc ctgactgctt ggatgtttgg
     2461 tctgcaaaac gtatcaaaga gcaaggcgct gatgccgtta agttcttgct ttactatgat
     2521 gtagacagct cagatgaact taaccaacaa aaacaagctt atattgaacg tgttggttct
     2581 gaatgtgtcg ctgaagatat tcctttcttc ttggaaatcc ttgcttatga tgaagaaatc
     2641 tcagatgcag gttctgttga atatgcaaaa gtgaaacctc gcaaagtcat tgaagcgatg
     2701 aaagttttct ctgatccacg ctttaacatt gatgttctta aagtagaagt accagttaac
     2761 gttaaatatg tcgaaggttt tgctgatggt gaagtggttt acagtaaagc tgaagctgct
     2821 gacttcttta aagcacaaga agaagcgact aaccttccat acatctacct aagtgcaggt
     2881 gtatctgcta aattgttcca agaaacattg caatttgctc acgactcagg tgccaagttt
     2941 aatggtgtgc tttgtggacg tgctacttgg gcaggatctg tagaacctta catcaaagaa
     3001 ggtgaaaaag cagcacgtga atggttgcgt actactggat tcgaaaatat tgatgaactt
     3061 aacaaagttc ttgttaaaac agctagtcca tggactgata aagtatagta aaaacataaa
     3121 acggaggata ttgttatgaa cagagaagag atgactctct tagggtttga aattgttgct
     3181 tatgctggag atgctcgctc taaactttta gaagcgctta aagcggctga aaatggtgat
     3241 ttcgctaagg cagatagtct tgtagtagaa gcaggaagct gtattgcaga ggctcacaat
     3301 tctcagacag ctatgttggc tcgagaagct tctggggagg aacttccata cagcgttacc
     3361 atgatgcatg gtcaggacca cttaatgact actgtcttat taaaagatgt gattcatcac
     3421 ctcatcgaac tttataaaag aggagcaaaa taatgcataa actcattgaa cttattgaga
     3481 aagggaaacc gttctttgag aaaatctctc gaaacatcta tcttcgtgct attcgtgatg
     3541 gatttatcgc tggtatgcca gtcatccttt tctcatctat ctttatcctt attgcctatg
     3601 tcccaaatgc ttggggattc cactggtcaa aagacattga gaccttcttg atgactcctt
     3661 atagctattc gatgggtatc ctagcattct ttgttggtgg tactaccgct aaagctttga
     3721 cggactcaat gaaccgtgac ctacctgcga ccaatcagat taacttctta tctacgatgc
     3781 tagcgtctat ggtagggttc cttttgatgg cagctgagcc tgcaaaagaa ggaggcttct
     3841 tgacagcctt cacgggaaca aaaggtcttt tgacagcatt tattgcagcc tttgtgacag
     3901 ttaatgttta caaggtctgt gtgaaaaata atgttactat tcgtatgcct gaagaggttc
     3961 caccaaatat ctctcaagta tttaaagact tgattccatt cactgtagcg gttgttctcc
     4021 tttatggatt tgaactcatt gttaagggaa ctcttggtgt aaccgttgca gaatcaatcg
     4081 gtacccttct tgctcctctc ttctcagctg cagatggtta ccttggaatt acacttatct
     4141 ttggtgctta tgccttcttc tggtttgttg gtatccacgg tccttcaatt gtcgaaccag
     4201 caatcgctgc tatcacttat gccaatatcg atgccaactt gcatcttatc caagctggac
     4261 aacatgcaga taaagttatc acttctggta ctcaaatgtt tattgttacc atgggtggaa
     4321 caggagctac attgattgtt ccattcttgt tcatgtggat ttgtaaatca gaacgtaacc
     4381 gtgccatcgg acgtgcctca gttgttccaa cattctttgg ggttaatgag ccaatcttat
     4441 ttggtgcacc aatcgtattg aatccgattt tctttgtacc gttcattttc gctccgattg
     4501 tcaacgtttg gatctttaaa ttctttgttg acacattgaa catgaactca ttctctgcca
     4561 accttccatg ggttactcct ggtccgttgg gaattgtact cggtactaac ttccaagttc
     4621 tatcatttat cttagccgga ctcttggtag ttgttgatac tatcatttat tacccatttg
     4681 ttaaggtata cgatgaacaa attcttgaag aagaacgttc tggtaaaaca aacgatgctc
     4741 ttaaagaaaa agtagctgca aacttcaata ctgctaaagc agatgccgtt cttggaaaag
     4801 caggcgttgc taaagaagat gtagcagcaa acaataatat cacaaaagaa actaatgtcc
     4861 ttgttctttg tgcaggtgga ggtacaagtg gcttgcttgc caatgccttg aacaaagcag
     4921 cagcagaata caatgttcca gtcaaagcgg cagcgggtag ttacggtgct caccgtgaaa
     4981 tgttgccaga gtttgacttg gttatcttgg ctccgcaggt cgcttcaaac ttcgatgata
     5041 tgaaggctga aacggataaa ttgggcatta aacttgcaaa aactgaagga gctcaatata
     5101 tcaaattgac acgtgatgga caaggtgctc ttgcatttgt tcaacaacag tttgattaat
     5161 aaatttgtaa aagttatttg agacgagatt attctttcag ttccttataa tattgttaac
     5221 catatcattg catttcattt ccttggtttc cggaactcag aatatcgtct caaatgcttt
     5281 ttttgaaagg atttatagtt atgactaaaa cacttcctaa agattttatt tttggtggtg
     5341 ctacagctgc ttatcaagca gaaggtgcga ctcacacgga tggtaaaggc ccagttgcat
     5401 gggataagta tcttgaagac aattattggt acacagcaga gcctgcaagt gatttttacc
     5461 ataaatatcc tgttgattta gaattagctg aaaaatatgg cgttaatggt attcgtattt
     5521 caattgcctg gtctcgtatt ttcccaactg gctatggcga agtaaatgaa aaaggtgttg
     5581 aattctacca taatcttttt gccgagtgcc acaaacgtca tgttgagcca ttcgtgactt
     5641 tgcaccactt tgacacacca gaagttcttc actcagatgg agatttctta aaccgcgaaa
     5701 atatagaaca ctttgtagat tatgccgctt tctgttttga agagttccca gaagtaaact
     5761 actggacaac tttcaatgaa attggcccaa ttggagatgg tcaatacttg gttggtaaat
     5821 tccctccagg cattcaatat gatcttgcca aagtcttcca atcacaccac aatatgatgg
     5881 tttctcatgc acgtgctgtg aaattgtata aagataaagg ttataaaggc gaaattggtg
     5941 tagtacatgc gttgccaacc aaataccctt atgatccaga aaatccagca gatgttcgtg
     6001 ccgctgagtt agaagatatc atccataaca aatttatctt ggatgcaacc taccttggac
     6061 actattcaga taaaacgatg gaaggggtca accacatcct agctgaaaat ggtggagaac
     6121 ttgatcttcg tgatgaagac ttccaagcac ttgaagcagc taaagatttg aacgatttcc
     6181 ttggtatcaa ctactacatg agtgattgga tgcaagcctt tgatggtgag actgaaatca
     6241 ttcacaatgg taagggtgaa aaaggaagtt ctaagtacca gattaaaggt gttggtcgcc
     6301 gaatcgcacc agattatgtg cctagaacgg attgggactg gattatttat cctgaaggct
     6361 tgtatgacca aatcatgcga ttgaaaaatg attatccaaa ctacaagaag atttacatca
     6421 ctgaaaacgg tcttggatac aaagatgagt ttgtagataa taccgtttat gatgatggtc
     6481 gtattgatta cgtgaagaaa cacttggaag ttttatcaga cgcaattgcg gatggtgcaa
     6541 atgttaaagg ttacttcatc tggtcactta tggatgtctt ctcatggtca aacggatacg
     6601 aaaaacgtta tggactattc tatgtagact ttgacacgca agaacgctat cctaagaaat
     6661 cagctcattg gtataagaaa gtagcagaaa ctcaagtgat agagtaatat tacttgaaaa
     6721 tgtaaaaaat tgttatccga tactttagaa aaagtacaac acttatttag accaggcttt
     6781 gaaaaggtat atattgttag tttcgaagat tgtcctatga ttcctgagct tgaagctacc
     6841 ctcgttgcta aaatgtggta aatggtatgt tttaactgga aaagagtgga tttgccattc
     6901 tgatttagag ttatcagcat ttgaatggga atttttacaa tcactcgatg tagaaatacg
     6961 tgagactatt cacttcgagg taaattattt gcctttccaa taataaaaag aaaggaatta
     7021 gttgtgacta tcgaattaaa aaacgagtat cttactgttc agtttaaaac acttggtggt
     7081 caactgactt cgattaaaga cgaggatggg atagagtatc tctggcaagc tgatccaaat
     7141 tattggaatg gacaggcgcc tattctattt cctatctgtg gtagtttacg aaatgattgg
     7201 gctatttata gacctcaaga aagacctttt tttacaggtc ttattcgtag acatggtttt
     7261 gttcgaaaag aagaatttat tctggaagaa gtcaatgata atagcgtgac ctttagtatt
     7321 aaaccaaatg ctgagatgct tgataattac ctatatcagt ttgaactgag agttgtttat
     7381 accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa cgaacttgga aatggaaaaa
     7441 acaatgcctt actttattgg agcccatcca gcattcaata gttctttagt agaaggtgaa
     7501 aactatgagg attattatat tgaatttagt gaggttgagt cttgctcgat accaaagagt
     7561 tttcctgaga caggattatt agatttgcaa gatcggacac cttttttgga aaatcaaaaa
     7621 attctcgatt tggactactc acttttttcc catgatgcca ttacacttga ccagctcaag
     7681 tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag gtcttcgagt agattttgat
     7741 gattttccga acttgattct gtggtctaca actaataaaa gtccattcat tgctttggaa
     7801 ccttggagtg gtctctccac ttcacttgaa gaaggtaatt tcttagaaga taaaagacag
     7861 gtaacgaaaa tttcacctca agaaacttct agaaaaatct atgatattac tatccttaat
     7921 taaaatttgt tattatttga gtcttcaata agatttgaat actcttacct tgtgatgata
     7981 gattggtctt ctccgtgcca gtcttacatc gcaaggtttt taagtttttt gtgggtctat
     8041 gtcaactgta gtgggtgacg aaaagctaat atctaaagac agtctggtag taaaaaataa
     8101 gattatttac tacttcttga tttaaaataa tagtggagcc attaatttgt ggcaaattta
     8161 atttaatatt ttgcctcaaa ttagccacac tttttataga actaagagga aaaatgaaat
     8221 tttcttccta ttaatatagt tgtttgaaat cttaaatata gcgtt
//
LOCUS       DAFGFZ010000121         8060 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000121 DAFGFZ010000000
VERSION     DAFGFZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.123"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            119..820
                     /locus_tag="LR726_002463"
     CDS             119..820
                     /locus_tag="LR726_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL6217502.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="LR726_002464"
     CDS             complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="LR726_002464"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL6217503.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="LR726_002465"
     CDS             complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="LR726_002465"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL6217504.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="LR726_002466"
     CDS             complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="LR726_002466"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL6217505.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="LR726_002467"
     CDS             complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="LR726_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL6217506.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4352..5332)
                     /locus_tag="LR726_002468"
     CDS             complement(4352..5332)
                     /locus_tag="LR726_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217507.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5356..6840)
                     /gene="galT"
                     /locus_tag="LR726_002469"
     CDS             complement(5356..6840)
                     /gene="galT"
                     /locus_tag="LR726_002469"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL6217508.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6852..7841)
                     /gene="galE"
                     /locus_tag="LR726_002470"
     CDS             complement(6852..7841)
                     /gene="galE"
                     /locus_tag="LR726_002470"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL6217509.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2280 a         1717 c         1416 g         2647 t
ORIGIN      
        1 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
       61 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      121 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      181 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      241 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      301 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      361 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      421 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      481 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      541 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      601 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      661 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      721 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      781 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      841 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      901 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
      961 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
     1021 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
     1081 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
     1141 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
     1201 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
     1261 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
     1321 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
     1381 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
     1441 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
     1501 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
     1561 tagttctacc tcagggagtt tagttgccaa agcatcctgc aatccttcac gcaacccttg
     1621 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
     1681 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
     1741 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
     1801 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
     1861 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
     1921 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
     1981 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
     2041 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
     2101 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
     2161 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
     2221 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
     2281 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
     2341 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
     2401 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
     2461 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
     2521 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
     2581 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
     2641 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
     2701 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
     2761 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
     2821 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
     2881 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
     2941 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
     3001 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
     3061 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
     3121 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
     3181 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
     3241 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
     3301 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
     3361 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
     3421 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
     3481 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
     3541 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
     3601 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
     3661 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
     3721 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
     3781 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
     3841 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
     3901 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
     3961 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
     4021 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
     4081 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
     4141 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
     4201 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
     4261 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
     4321 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
     4381 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
     4441 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
     4501 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
     4561 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
     4621 aaagcgcatc aggaacatgc ggattcgttt ttaaatactg cttcactgct tgatatcctg
     4681 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
     4741 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
     4801 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
     4861 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
     4921 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
     4981 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
     5041 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
     5101 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
     5161 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
     5221 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
     5281 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
     5341 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
     5401 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
     5461 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
     5521 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
     5581 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
     5641 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
     5701 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
     5761 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
     5821 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
     5881 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
     5941 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
     6001 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
     6061 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
     6121 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
     6181 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
     6241 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
     6301 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
     6361 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
     6421 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
     6481 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
     6541 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
     6601 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
     6661 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
     6721 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
     6781 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
     6841 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
     6901 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
     6961 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
     7021 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
     7081 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
     7141 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
     7201 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
     7261 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
     7321 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
     7381 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
     7441 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
     7501 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
     7561 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
     7621 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
     7681 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
     7741 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
     7801 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
     7861 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
     7921 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
     7981 aacattttca aggatttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc
     8041 tcttttgttc cataatagat
//
LOCUS       DAFGFZ010000122         8027 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000122 DAFGFZ010000000
VERSION     DAFGFZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.124"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..434)
                     /locus_tag="LR726_002471"
     CDS             complement(<1..434)
                     /locus_tag="LR726_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL6217510.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYP"
     gene            complement(448..1698)
                     /locus_tag="LR726_002472"
     CDS             complement(448..1698)
                     /locus_tag="LR726_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL6217511.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(2076..3527)
                     /locus_tag="LR726_002473"
     CDS             complement(2076..3527)
                     /locus_tag="LR726_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL6217512.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(3848..4612)
                     /locus_tag="LR726_002474"
     CDS             complement(3848..4612)
                     /locus_tag="LR726_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL6217513.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4640..5338)
                     /locus_tag="LR726_002475"
     CDS             complement(4640..5338)
                     /locus_tag="LR726_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL6217514.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5350..6129)
                     /locus_tag="LR726_002476"
     CDS             complement(5350..6129)
                     /locus_tag="LR726_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL6217515.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6145..7089)
                     /locus_tag="LR726_002477"
     CDS             complement(6145..7089)
                     /locus_tag="LR726_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL6217516.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7135..7914)
                     /locus_tag="LR726_002478"
     CDS             complement(7135..7914)
                     /locus_tag="LR726_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217517.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2265 a         1679 c         1308 g         2775 t
ORIGIN      
        1 ttaggataaa attgtagcat agtttcccac tgaacataaa attgatctgc aaaatgatac
       61 atcaattttc ctgtctgagt agggctggtt acttttgcaa acgattcaat ataaatcagt
      121 ttttttccca tcatttttgt tattaagcag atagggatca tagctaaaac acctgtacaa
      181 atcacgagat ccggtttttc ctttatatat ataataattg atttgaataa attaataagt
      241 atagtacaca aaaaaaactt ttcttttcta tttacttgtc tcatgtagta cgttttttcg
      301 ccttttgact cagctgaata ttctgtctgt tctgttataa caaaactctc atacttctcc
      361 atcagtggtt ttaacatcat taactgttca taatgccccc cagaggaagc cgcaaaacat
      421 atctttttgt tcatattaac ctctctatca gtcacgctga aaaatatctc tagtgtaaac
      481 tttttcttta atatcatcca agcaggtatc atatcgattg gcaataatag aatcacacat
      541 cgccttaaac tgctctaaat cgttgataac cttacttccg aaaaaagtct ctccatcttt
      601 cagtgcaggc tcataaatca ccacagtcgc acctttagct tttacgcgct tcataactcc
      661 ctggattgaa ctctgacgaa aattatcact gttgctcttc atggtcagcc ggtatatacc
      721 gattaaaact ggattttccc tgttgacatc atactgatta tattcagcgt agttgtaata
      781 gcccgccatc tgaagaacac gatctgcgat gaaatcctta cgggtacgat tagattcaac
      841 gatagcctca atcaaatttc caggaacatc tgcatagttt gccaacaact gcttggtatc
      901 ttttggcaag caataacctc catatccaaa ggatggattg ttgtagtgag ttccaatacg
      961 aggatcgaga caaacaccat tgattatctg ctgagtattc agccccttca tttcagcata
     1021 agtgtctaat tcattgaaat agctgactcg cagtgctaaa taggtatttg caaacagctt
     1081 tactgcttcg gcttcagtga atcccataaa aagagtatcc acgttctcct taattgcact
     1141 tttctgcaga agtgttgcaa aaacatgagc agcatttaac aagcgctcat cagtcatatc
     1201 agtaccaaca ataatacgag atggatacag attatcatat aaagccttgc tctcgcgcag
     1261 aaactctgga ctaaaaataa tgttcttact ccccgttgtc tcacggactg attcagtgta
     1321 gccaacagga attgtagact tgatgaccat gatggtatca ggattcacgt ccatcacaac
     1381 ctcaataact gactcaacag cagaagtatc gaaatgctga gtacgactgt catagttagt
     1441 cggtgtagca ataacaacga aatctgcgga tttataagcg ctccttgcat ccaaagtagc
     1501 agttaagtca agttcttttt ctgtaagata cttttcaata tattcatcct gaattggaga
     1561 cttccgttta ttaatcagtt caatcttttc agggataatg tctaccgcgg ttacctggtg
     1621 atgctgcgcc aatagtgtgg cgatactcaa accaacataa ccggtgccgg ctactgcgat
     1681 gttgtacttt ttcttcacaa tatctcctct ttcctctctt aagtatggag aatgttactc
     1741 ctacggctat tcatcatgtc aaaataacac agcttagaat gttgataaaa taattttcca
     1801 gaatgtgata aaatctcttt catttataaa ccacacatct aattttatct attctccaca
     1861 agacatgtag agtattcttt aataacaaga tacgacctta ctaaatactt caaaaaatta
     1921 tcatatttaa ttcataaata taaatcttca ttttaaatgt atgcccaaaa atttatctgt
     1981 aatgcgtcat caaaatctag attccaaatc tttatcaatt tttatccact cattctacca
     2041 ctttagtaat attttctatt tataacactt tcgtcctaca ttgatccccc cctctgaagt
     2101 acagttttta ctgtcttaaa taaaatttta aaatctaacc ccatactcca attttttata
     2161 tattccgtgt caagttttgc tacttcttca aaatcagtaa tatcacttct accacttacc
     2221 tgccacattc cagtaattcc tggcttaata gcaattcttg cacgatgatg ataatcatat
     2281 tcttcatact ctgatttaag gcatggtcga aatcctataa ggctcatctt tcctgttaga
     2341 acgttgaaaa attgagggaa ttcatctaaa gacgtggatc taataaattg accgattcct
     2401 gtcttttttg ttccatctgg aagtatttta tttccgataa ttcttggatc aaactccatc
     2461 ttaaacataa gattattgct catcttattt tctttcatta attctacttt tctttcttct
     2521 gcatccatat acatacttct aaacttatac atcttgaacg gtttaccatt ctgacctata
     2581 cgttcttgtg caaagaaaat tgggcctggt gaattaatat aaataagtgg agcaataaag
     2641 ataaaaataa ttcccgttgc aatacatcca acaatacctg caagaatatc aattgttcgt
     2701 tttataaatg cctgttgagt tgtcataaca ttgatacttg ttgtaagtac tgtatattct
     2761 cctatacgac caatagattg ttttcccaaa gcactatctg atacttttgc aagattcaaa
     2821 tgtacagtaa ggcccatttc agagcatcgt tcaataaatt ctttcataca cgaagtactt
     2881 ttattcaaaa taataaagac ttcatctacc catccctgac ataagtaatc cgctgcattt
     2941 ttggaatcag aaacaactgt cactccctca acatcttcac caatcatgct tttatctatg
     3001 ataatgacac catttatttt atacatttta tagttatgct ctttcacatt acgaataact
     3061 acttcagcaa tactactaga tgtaactata ataagtgatt tgttcccctc atttttcatt
     3121 ttatgtttca agtaatgttt ccataagagg cgagttatat atgtaattac aacgtataaa
     3181 cctcccatca agaaaagaac gactctagaa taatcatttc caccctgaac actaatgaga
     3241 tacaaacttc ctaacagcac caacataatc gcttgcttta cagaagaagc aaattctata
     3301 tagtaccctc tttttaatac acctctatat gcttcaaaca tgaaaataac taaaagatct
     3361 gccagttcaa tgaagactgc catgttttga tacattggaa tcacataagg attccaatct
     3421 acctggcgta agacatacga tagataaaaa gcaagctgga tacacaataa atctaaaata
     3481 ataaaatcaa tatgtttaac ccatcccctt gaacttttct tgtacattcc tttttactcc
     3541 cctcttttta ataaaataaa ttgtattact tgttttctct cctaaatatc tttctgaagg
     3601 attattgact cgacaaattg gcttgtggat attcttatat atagaacatc cgtaagccaa
     3661 cttaaaactc cttaacaaaa tttttttact catattgtag atcaaaacaa gtatccaaga
     3721 aatcatattt tatttctttt ttctgcttac tcaacaacag ctaaaagaaa aatcatcata
     3781 aacaaattca actaagccaa cgatcccctt gtgaaatcaa gttttttctt gatttacgaa
     3841 taaagagcta aaataaacca aacttcttct tttttacctc ttcatagact ggataactga
     3901 tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac
     3961 caaagtcctt tgcaatctgc tcatagcact cttttaagta aaaggtgcgc ttagagaccc
     4021 catgggcatc agaagcagcc atctgcacga gattatgttt gaccatcgtt ttggctgttt
     4081 tctggatgtc tttgccaaat accccaacat aacttggcgc agtgagttgg gccaaacatc
     4141 ccatatctag gaacggaagc aaatgatttg ggtcttcgcg aaaatgcacg ttgcgttctg
     4201 gatggacgat tactggtgtc tttcctagtt ctaacaactc aaaaaaaagc cgttctgcat
     4261 ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct aaatctgtaa
     4321 ataatatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt acttcttgtc
     4381 cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac gaagcgacta
     4441 gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt ggtgtgcata
     4501 aaatatgcgt gatgccttgc tgaatcgctt tttcagccat tgcaatactt gcttctaagt
     4561 tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc atttctcttg
     4621 tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg ctatgttctg
     4681 ctccgttgta cacaacacct aagactcgtg cttgtaccat ctccagcagt tcttttgctt
     4741 tagtcaagga ttctttacgt gacatatttt cacgtacgac caataaagtg ccatccgttt
     4801 ttaaagacat gatctgtgca tctgttacgg ctactactgg tggcatatcg aatatcacca
     4861 cgtcaaataa ctgtcttgct tcggatagga tctgatccat tcttggcgag cttaacaatt
     4921 ctgatgggtt gggtggctta ggtccactag gcaaaatcga caagttctcg actggcgttc
     4981 gttggatcac atctgctaca cttcctgatg aactaagtgc tgtacttaat ccactggcat
     5041 tgttcagctg gaatgtctta tagacgacgg gtttacgtaa atctgcatcg actagtagaa
     5101 ctcgttgacc tgatttagca aaaacgactg caatgtttgc tgcagtggtg gatttccctt
     5161 ctccaggacc tgatgaagtc acgacaatcg ttttgatttg ctgaccagct gcggaggcaa
     5221 actggatatt cgtccggatc gtacgatatt gttcggcgat tggtgaagat gggtttgtta
     5281 atgtgatcaa actgactgca tgagtttgtg ctgctttttg tttctgtgtt cgtgccattt
     5341 ttcttcctcc tagactcttg agcggcttct gcgtgatggg gctgttttat cgtttgtagc
     5401 ttttggtcgt gcaggagctg ttcctttttt acggatacca gcgttcaatt ctttcgctga
     5461 catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat atttttcatc
     5521 tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta ccatcattcc
     5581 taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg tcgtattggc
     5641 aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg cgttctcttg
     5701 gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg tacttattgc
     5761 ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat ctttgatttg
     5821 ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt cattgagcac
     5881 taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc
     5941 attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta
     6001 ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga
     6061 gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt
     6121 ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc
     6181 ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat tatcttgcat
     6241 gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc gataatctaa
     6301 agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact ctagagcatt
     6361 caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg ctacgccttc
     6421 tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt cataacgcat
     6481 acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata
     6541 gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag
     6601 ttcctttagt cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt
     6661 ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc
     6721 ttttgtgtca cgacctacga tgtcgacata cgtatctcga ggaatgctga cgatcgttgt
     6781 ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac
     6841 ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt cttgtttgtc
     6901 cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc
     6961 aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa
     7021 tacgataaga actgctaaaa taatcaaaaa tatttttttc cctcttgatt ttcttcttcg
     7081 tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata
     7141 gcttatattg ctatagccca tttgctgagc aacagtttgt gcttttgttg cgtatgcggc
     7201 tcttattttt tcattccgga tctgctctat ttgactgagc gccgtcaaat attgatcgta
     7261 agtagcggta gctgttactt gaccgtcttt cagcatcgta tcgaatagct gatctagttg
     7321 cgtgacttgt gcaatttggg tatcagcgaa tcctagatat tgcataacga ctgttttcca
     7381 agcactatcc ttaaaaaagc tcatattttc acgtacatgg gcgacatctg cacttgccaa
     7441 cttttctttg atgatgacat catccacagc ttgctgccag tttgatacgt ttttagtaaa
     7501 taatttattg acagccgtct ggatcttcag ttttgattct gcatcctcta actgcgttaa
     7561 gactgctttc ttttcttctt ggatcgcagc agcttcttta ggtaattctg attccttgat
     7621 gtcataatca tctgccgaaa gtttgataga agatagttta tgtgacaaat ttgaaactgt
     7681 ttcttctgtt atttcaggat ttaggaaaat tttttctttt gtactgtaca gcttatctac
     7741 ttcttttgat aaagaggtca cggtgtcttt ttgattggtg atggcagatt ctgcgtaatt
     7801 gatccgttga tgatattgcc gctgattatt gagatggtat ccaaacaagc aaatcacaat
     7861 agcgcctgtg cctaccgcca cagtagccaa taacttgacg aacggctttt ccatagattc
     7921 actcctaaat attgatgata gataagtaaa gatgtgattc attcacatca gaatgagcgc
     7981 tttttatttt aaaagcataa ttaaactaat atattcatag aaatatt
//
LOCUS       DAFGFZ010000123         7908 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000123 DAFGFZ010000000
VERSION     DAFGFZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.125"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            234..2000
                     /locus_tag="LR726_002479"
     CDS             234..2000
                     /locus_tag="LR726_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL6217518.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2102..3361
                     /locus_tag="LR726_002480"
     CDS             2102..3361
                     /locus_tag="LR726_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL6217519.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3508..4809
                     /locus_tag="LR726_002481"
     CDS             3508..4809
                     /locus_tag="LR726_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6217520.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4812..5663
                     /locus_tag="LR726_002482"
     CDS             4812..5663
                     /locus_tag="LR726_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL6217521.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5676..6491
                     /locus_tag="LR726_002483"
     CDS             5676..6491
                     /locus_tag="LR726_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL6217522.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6642..7808
                     /locus_tag="LR726_002484"
     CDS             6642..7808
                     /locus_tag="LR726_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL6217523.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2580 a         1366 c         1583 g         2379 t
ORIGIN      
        1 cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag
       61 tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa
      121 aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg
      181 cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata
      241 cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat
      301 ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag
      361 atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc
      421 taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac
      481 agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa
      541 tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt
      601 ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa
      661 tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt
      721 ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg
      781 atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta
      841 aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg
      901 gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca
      961 tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca
     1021 aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt
     1081 cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt
     1141 ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg
     1201 atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta
     1261 atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag
     1321 acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat
     1381 tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga
     1441 aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc
     1501 aggtgaacga aggaacattc aacttattag attctcatga tactgccagg atcttgaccc
     1561 tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag
     1621 ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt
     1681 gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa
     1741 aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt
     1801 ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga
     1861 tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg
     1921 tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg
     1981 gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga
     2041 agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa
     2101 gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg
     2161 cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa
     2221 aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac
     2281 tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt
     2341 ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata
     2401 tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga
     2461 agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga
     2521 acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc
     2581 atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga
     2641 agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt
     2701 aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg
     2761 tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt
     2821 ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat
     2881 gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa
     2941 caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc
     3001 gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca
     3061 aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa
     3121 tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac
     3181 aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg
     3241 cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc
     3301 aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg
     3361 attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg
     3421 caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg
     3481 tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa
     3541 aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct
     3601 tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc
     3661 ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga agatcattca
     3721 ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt
     3781 tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa
     3841 atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta
     3901 ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca
     3961 ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag tttaattgat
     4021 tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt
     4081 tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc
     4141 cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc
     4201 ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg
     4261 aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag
     4321 cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta
     4381 gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga
     4441 atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca
     4501 tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt
     4561 attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc
     4621 ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat
     4681 aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc
     4741 tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg
     4801 ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact
     4861 tttttactta cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca
     4921 cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg
     4981 actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata
     5041 aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg
     5101 cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt
     5161 tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct
     5221 tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc
     5281 caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat
     5341 ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg
     5401 tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca
     5461 tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa
     5521 catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga
     5581 ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga
     5641 catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt
     5701 tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta
     5761 aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt
     5821 atgccaatca gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg
     5881 atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat
     5941 cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac
     6001 aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa
     6061 aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt
     6121 ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc
     6181 gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga
     6241 gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat
     6301 ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca
     6361 tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa
     6421 caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc
     6481 gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata
     6541 aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt
     6601 agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat
     6661 acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta
     6721 caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag
     6781 aaatcccaat gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg
     6841 atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag
     6901 caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata
     6961 cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata
     7021 aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata
     7081 cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag
     7141 aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca
     7201 aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta
     7261 ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc
     7321 gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag
     7381 gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa
     7441 cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata
     7501 aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa
     7561 aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag
     7621 aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt
     7681 ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta
     7741 atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc
     7801 gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg
     7861 atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaa
//
LOCUS       DAFGFZ010000124         7811 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000124 DAFGFZ010000000
VERSION     DAFGFZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.126"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            340..813
                     /locus_tag="LR726_002485"
     CDS             340..813
                     /locus_tag="LR726_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL6217524.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            810..2942
                     /gene="feoB"
                     /locus_tag="LR726_002486"
     CDS             810..2942
                     /gene="feoB"
                     /locus_tag="LR726_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL6217525.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2944..3078
                     /locus_tag="LR726_002487"
     CDS             2944..3078
                     /locus_tag="LR726_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL6217526.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3172..3816
                     /locus_tag="LR726_002488"
     CDS             3172..3816
                     /locus_tag="LR726_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6217527.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4003..5196
                     /locus_tag="LR726_002489"
     CDS             4003..5196
                     /locus_tag="LR726_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL6217528.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5189..6916
                     /locus_tag="LR726_002490"
     CDS             5189..6916
                     /locus_tag="LR726_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL6217529.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7310..7507
                     /locus_tag="LR726_002491"
     CDS             7310..7507
                     /locus_tag="LR726_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL6217530.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7509..>7811
                     /locus_tag="LR726_002492"
     CDS             7509..>7811
                     /locus_tag="LR726_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL6217531.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
BASE COUNT         2580 a         1310 c         1708 g         2213 t
ORIGIN      
        1 aaaaagtcaa acaacaaaca ctacatattg tgttgtggaa actgtggaat actaagtttt
       61 gtgtttttga aaaaagagct gaaaacaagt atcgtgtggg aaaatagcaa atttaataga
      121 gaaaaaattg tggaaaacta tgaagaaata tttttttctc atttttatct atcaatacta
      181 aagattcgct cataatatca caagtataat tgacattctc aattaccgag agtattctat
      241 gtgagaatga ttctcattta tttgcgtaac acgtaagaaa attagaacat ataatgaatg
      301 attttaatac atgatttatt tcacaagaga ggaatcaata tgcagacctt agcgcaggca
      361 aagcagaatt tcgtttatca agtcgaacga atcgaaacag aagaaaaaac aaaacgccat
      421 ttgcaaaata tcgggatcgt tgccggaagt aaaatcgtcg tcgtcaattt atcaggcaac
      481 aatgccattt tactggtaaa aagtcatcga gtggcagtaa gtcgggaaat actaggtcaa
      541 atctttgtca ataaaaagac tgcttcctct gaaagctgga cttctcttga tcaattaaaa
      601 gcaggagaga ctgggatcgt cgtagcaatc cacggacaag gcgcagtgaa gcgacggtta
      661 atggatatgg gattgacgcg aaatgtaaaa atagttatcc gaaaactagc ccctctggga
      721 gatccaatcg aactgacagt gcgagggtat gaactgacgt tacgaaagtc agaggccgaa
      781 ctaatcttga tacaaaggga ggaaaaagaa tgaatatcgc gttagtcgga aatccaaaca
      841 gcgggaaaac tagcttgttc aatctcttga ctggttcgaa tcaatatgta gggaattggc
      901 cgggcgtaac agtagaaaaa aaagaaggat cgttgaaaaa aaatccgaac attcatattc
      961 aggacctacc cggcatctat tctttgtctc cttatacacc agaagaagtg attgcaagag
     1021 attatttggt tcaggaacag cctgaactga tcatcaacat tattgatgct accaatttag
     1081 aacgaaatct ttatttaact acgcagttat tggatttaga cattcctatg atcattggtc
     1141 tgaatatgat ggatgtcatc aaaaaagaag gaaaaaaaat caatctagaa aagctttcct
     1201 atggtcttgg tgtaccagtt ttaccaatca gcgtattgaa aaagcaagga atcgaaaaac
     1261 taatcgaaaa gattcaggag tctgcaaaaa agcggccttc tattcctgtt ccctgtacat
     1321 atgatccacg tttagaagca gctttacatg aaattgaaga tgtactagga gaacaaagcg
     1381 gcaagaaacg ctggtatgca atgaaatatt ttgaacgaga tcagaaggta agagaggatt
     1441 acgagatagc agtttctcag caaaaagaga tcgagcaact gatccagtta acagagaaac
     1501 tattggacga cgatagtgag acgattctgg tcaatgaacg ttatgaattt attacccaac
     1561 tatgtacact ctgtgttgtg tcaaatgatt ctttccaatt atcgatgagt gacaaaatcg
     1621 atcagattgc aacgaatcgt tggttagcct tgccgatctt cgcatttgtg atgtggctga
     1681 tctattatct ggctattcaa acagtaggaa caatggggac agattggatc aatgacacat
     1741 tgtttggtac atggttacca gaacatgttg gcagattgtt agctgaatgg caagtagcgg
     1801 gatggatgca ggagctgatc ttgaacggca tcatcgcagg cgtgggagca gtgttaggat
     1861 ttctaccgca attgattata ttgtttttat gcttgagctt tctggaagac tgcggatata
     1921 tggcgaggat cgcttttgtc atggatcgac ttttcaggaa attcggtctt tcaggaaaat
     1981 ccttcattcc tatgctgatt gctacaggat gtggagtgcc aggcgtcatg gccagtcgca
     2041 cgattgaaaa cgagcaagat cgtcgtttga cgatcatggt cacaaccttc atgccttgtt
     2101 ctgcaaagtt gccgattatt gccttagtag caggggcctt ttttcctcat caatcttggg
     2161 tagctccttc tgcctatttt cttggtatga ctgcaattat tttctctgga atcagcttga
     2221 aaaaaacacg tttgtttgct ggagatacag ctccttttat catggaattg cccgcatatc
     2281 attttcctca atggtcagcg gtcctaagac aaacctatga ccgcagcaag tcttttgtta
     2341 aaaaagcagg aacgattatc tttgtttcta gtatcgtgat ctggttcagc tcttcttata
     2401 atttttatgc tcaacctgtt tctgaagacc aaagtatctt agcctttttc gggcgaatcc
     2461 tggcagttgt ttttcagccg cttggttggg gaaactggca aggaacagtc gcagcgatta
     2521 caggattggt cgctaaagaa aatattattg gcacatttgg tattttgttt ggtcatgcgg
     2581 aagtatccga aaatggcaga gaaatctggc aagtgctaca acacacatat actcccgcag
     2641 cagcttattc attgttagcc ttcaatttac tttgtgcgcc ttgttttgcg gcaattgggg
     2701 ctatccatcg ggaaatgaat gccaagaaat ggacttggat cgccataggc tatcaatgcg
     2761 gtttagctta tttagttagt tttgtgattt atcagttggg acacttattg gagggtggac
     2821 aggtagcgtt agggacgtat cttgctattt tactgatgat aggactcgtt tatgttttgt
     2881 tccgtcagcc aaaatccaag aaccagtttt atactatatt gtcattagaa ggagaggaat
     2941 aggatggcga cattcttatt aagtgtgtta atctttggaa gtgcaggcgc aattgtctat
     3001 actcgtttaa aatccggtaa gaattgtgat gactgtcaaa cagcttgccc agtaaaaaag
     3061 gaacagatca agcgatgatc agtcgttgca agaggctatt ctcccctttt ttgcttattt
     3121 tgctagagca tagtaagatg agggaaagta aaaaaagaaa gaggaaagaa aatgtctgga
     3181 aagaaaaaaa tcgtccgccc gcgctaccag cagattgctg ttgatttagc cgaacgtatc
     3241 gttgaaaacc gatataaagt gggtgagaag atccatgccc gctcaacact tgccagtacg
     3301 tacaatgctt ctcctgagac tacccgcaag gctatcaatg tgctggtaga tcttggaatc
     3361 atggaagtac ggcatggaag tggggcattt gttgcttcga aggaaaaagc aaaagatttc
     3421 ctattgcaat atcaagatgt acaatccatc caagatacaa aagcagaaat catggcaagt
     3481 gtggagaagc aacaagatga gctgaatcat ttttctcaat tattggatca gctggtcaat
     3541 caaacgaagc gaatacataa gatgaatcct ttacttccgt ttgaattatc tttatcagaa
     3601 aaagcaatgc atctggaccg ttcaattagc gagttgaata tctggcaggc gactggcgcc
     3661 acagttatcg caatttctca taatgaagaa ctgcttgttt cacctggccc ctatgctaaa
     3721 tttacggcag gggacatcgt tcattttatt ggaaatgaat atacactaca gcgtatgaca
     3781 aacttttttt acccggagca tcagcttgca gaatgattgt gtgaaatatt aaaaaaatat
     3841 taacttggct aaacctttta tttacaaaga tttagccaag tttttttcag tttggacaag
     3901 caaatttctt tcatttttga caaagtgact acatgatgtt aacatagtgt ggtcactttt
     3961 ttcagaaaga aaaatgaatt tacataaaaa aggagaattt gtttggtaaa agtagaagta
     4021 aaacatttaa caaagatatt tggtaaaaag acacaagccg ctttagatat gatgaatgat
     4081 caccagccga aaacggagat tttaaagaaa accggtgcaa cggttggtgt gtatgacgtg
     4141 aattttgacg taaaagaagg agaaatcttc gtgatcatgg ggctttccgg gagtgggaaa
     4201 tccacattga tccgtctatt gaatcgtctg attgagccaa cttcgggcag tatctatatt
     4261 gatggagaag atgtatcgaa gttaagcaaa gaagaattgc gagaagtccg tcgtcataaa
     4321 atcaatatgg ttttccaaaa cttcggtctg tttccacatc gaacgatctt ggaaaataca
     4381 gaatacgggt tagaagtccg tggtgtacct aaagaagaac gacaagaaaa agcagaaaaa
     4441 gcattagaaa attctagttt gctttcattt aaagaccaat atcctagtca gttatctggt
     4501 gggatgcaac aacgggttgg cctagccaga gctttagcaa atgatcctga gatcttgctg
     4561 atggacgaag cgttctcggc tcttgatccg ctgatccggc gagaaatgca agacgaatta
     4621 ctggacctgc aagccaatgt tcaaaaaacg attattttca ttacccatga tttgaatgaa
     4681 gctttacgga ttggggacag aatcgccttg atgaaagacg gcgaaatcat gcagatcgga
     4741 acaggtgaag aaatattgac caatccagca aatgattatg tacgtgaatt tgtggaagaa
     4801 gtcgatcgtt ccaaagtgct tacggctcaa aacatcatgg tacctgcatt gacgacgaat
     4861 atcgaatctg atggaccaaa cgttgcattg acaaggatgc gtaatgaaga agtcagcatg
     4921 ctgatggctg ttgacagaaa gagacacctg aaagggatca tcactgcaga tcaagcgtta
     4981 gaagctagga agcaaaaacg tccattgatc gacttcctag atgaaaatgt gacggtgatt
     5041 gggaaagaca tggtggtaag tgatattttc aatattattt atgactcacc gactccactt
     5101 gcagttgtag aggatggaaa attaaaaggt gtcgtcatcc gaggtagtgt gattgaagca
     5161 ttagcagaaa caagcgaggt gagtgaacat gaataatcta ttgaactatc aattaccagt
     5221 ggcaaactgg gtagaaaata tcacagaatg gtttacgaca actttttctg gcctattttc
     5281 atttttacaa acgattggac aggcagtgat gtcggggatt acaaatttac tgttagtgat
     5341 tccagctcct ttgtttatct tgttattgac ggtagcagct ttctttattt ctaagaaacg
     5401 tccaggactt acgttgttca cattaatcgg tctatggttt atttataacc aaggtctatg
     5461 gaatgatttg atgaatactg tcacacttgt tcttttatct agtgtgatct cgatcatcat
     5521 tggggtacca ttagggatct tgatggcaaa aagcagtaaa gcccaaagta ttatcaaacc
     5581 gatccttgac tttatgcaga caatgccagg attcgtttat ttgattccag cagttgcatt
     5641 tttcggtatc gggatggttc caggggtatt tgcctcagtc atctttgctc tgccaccaac
     5701 cgttcgtttt acgaatttag ggattcgaca agttcctaaa gaattagtag aagcttctga
     5761 ttcattcggg agcacaagtc gtcaaaaact attcaaacta gaattgcctt tagctaaaag
     5821 tacgatcatg gcagggatca atcaaacaac aatgctgtca ctttcaatgg tcgttattgc
     5881 ctctatgatc ggtgcacccg gcttaggacg cggcgtatta tcagcattgc aaagagcaca
     5941 agtcgggaat ggatttgtca acggggttgc attagttatc ttagcaatca tcattgaccg
     6001 ttttacacag catttgaacc agccaaacaa taaaaaagct gcaggagcaa caacacagaa
     6061 aagtaaaaaa cgtcaaggac tgatcattgg tgctgtagta gtagtgatct taggtgcgat
     6121 tggtataggg agtttttcat ctgccaaaga aacaaaacgt atcaacttat cttatgttga
     6181 atgggataca gaagttgctt caacaaatgt agtcggtgaa gtattgaaac aaatgggcta
     6241 tgatgtaacg atgacgccat tagataattc aatcatgtgg aaatctgttt caaatggcga
     6301 gtcagatgcc atggtttcag cttggctacc taaaactcat ggttcacaat atgcacaata
     6361 taaagatcaa gtcgaagacc taggtgccaa tttgacagga gcaaaagttg gtttagcagt
     6421 gccagcctac atggatgtaa attcgatcga tgagttaacc gatcaggcag gtaagaaaat
     6481 cataggaatc gagccaggag ctggtgtagt tacggctgct gaaaacacga tccaaaaata
     6541 tgacaacttg aaagactgga aagtagagac atcttcttca ggagcaatga ccgttgcact
     6601 aggtcaagcg atcaaaaaac acgaaccaat tgtagttaca ggatggacac ctcattggat
     6661 gtttgccaaa tatgatctga aatacttaga agatcctgaa aacggaatgg gaagcgaaga
     6721 gcagatccat acgatggttc gaaaaggatt gaaagaagac cagccagaag cttacaaagt
     6781 attagacaat ttccattggt cagaaaaaga tatggaaaaa gttatgttgg aaatcaacaa
     6841 tggaaaagat ccacagcaag cagcaaaaga ctggatcaaa gagaaccaag aattagtcga
     6901 aagctggaaa aaataaaaag acagacacga aataaatgga gcttccaaaa ttggatcaaa
     6961 tatccaaatt tggaagctcc atgttcgtct ctatcttttt tgtctataaa aaaagagaat
     7021 gcgtagctca tcttcgaaac gtccaaagac gccgacaaac tattatgaat ttatgggtgc
     7081 gagtcaaaga gatcgatcgt aggtatgaaa gactaatgtg attaaagttt attatgtacg
     7141 aagtgcattc tcaaaaaata ttaaagaata tacatagttt atatacttaa aagtgattaa
     7201 attcttattc aggaattatt ttgctacgtt tcaagaagga attgtttttt gaaatttaat
     7261 gaatgttaga ataatagtct aaaaacatta atttaggggt gattagatta tgaaacattt
     7321 aaaaattttg tctattaaag agacacaact tatctatggg ggtaccactc atagtggaaa
     7381 atattatgga aatggagtgt attgcactaa aaataaatgt acggtcgatt gggccaaggc
     7441 aactacttgt attgcaggaa tgtctatagg tggtttttta ggtggagcaa ttccagggaa
     7501 gtgctaaaat gaaaaaaaat gctaagcaaa ttgttcatga attatataat gatatatcta
     7561 taagtaaaga tcctaaatat tctgatattc ttgaggtttt acaaaaggta tatttaaaat
     7621 tagaaaaaca aaaatatgaa ttagatcccg gtcctttaat aaatagattg gtgaattatc
     7681 tatattttac tgcttatact aataaaataa gattcactga atatcaagag gaattaataa
     7741 gaaatttgag tgaaattgga agaactgctg gaataaatgg tttatatcga gcagattatg
     7801 gagataaatc t
//
LOCUS       DAFGFZ010000125         7690 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000125 DAFGFZ010000000
VERSION     DAFGFZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.127"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            111..2720
                     /gene="clpB"
                     /locus_tag="LR726_002493"
     CDS             111..2720
                     /gene="clpB"
                     /locus_tag="LR726_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL6217532.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2871..3557)
                     /locus_tag="LR726_002494"
     CDS             complement(2871..3557)
                     /locus_tag="LR726_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217533.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3535..4029)
                     /locus_tag="LR726_002495"
     CDS             complement(3535..4029)
                     /locus_tag="LR726_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL6217534.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4049..5269)
                     /locus_tag="LR726_002496"
     CDS             complement(4049..5269)
                     /locus_tag="LR726_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217535.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5416..7251
                     /gene="lepA"
                     /locus_tag="LR726_002497"
     CDS             5416..7251
                     /gene="lepA"
                     /locus_tag="LR726_002497"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL6217536.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7447..>7690
                     /locus_tag="LR726_002498"
     CDS             7447..>7690
                     /locus_tag="LR726_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217537.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYR"
BASE COUNT         2564 a         1439 c         1560 g         2127 t
ORIGIN      
        1 tcaaaaaact atgtgtatat tttgaccaaa attgaccaaa gcgatataat agtttttgta
       61 aagatcaaag aaataagtga tcttaccttt aattaggagg gtatacctgt atgaatattg
      121 agaaaatgac tacgacgctt caagaagcga ttgctgaagc tcaacagatt gcggtcactc
      181 gaaaacatca agatatcgac attgcccatg tatggaaaat ctttttacaa tctaatcatt
      241 ttggtcgaaa tttctatacg gatgccggat tggatgtcga atcttttgaa catgaaattg
      301 atcgtctact ggatgagtat ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc
      361 tcagccagaa tttctttcga ttattgaatg aagcagacca aatacgtgaa agcttcggcg
      421 acgagtttct ttcaacggaa gtcgttattt tagcgttgat gaaattaaaa aattatccgt
      481 taactgttta tttgaataaa aacggattat ctgaaaaaga attgcgtaaa aacatcgaag
      541 agatgagagg aggagaaaga gtgacctcac aaaaccaaga agaacaatac aaagcattag
      601 aaaaatacgg cgttgattta gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg
      661 gtcgtgatga agaaatccgt gatgtcatcc gtattctttc acgaaaaaca aaaaataatc
      721 ctgttctgat aggagaaccc ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac
      781 gtatcgtacg caaagatgtg cctgaaaatt taaaagataa aacgattttt tcattagata
      841 tgggtgcatt gatcgctgga gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag
      901 tcttgaaaga agtcaaaaaa agtgatggac gtatcctctt gttcatcgat gaaatccaca
      961 atatcgtagg ggcaggaaaa acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa
     1021 tgcttgctcg cggtgaatta catttgatcg gtgccacaac tttagatgag taccgtcaat
     1081 atatggaaaa agacaaagcg ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa
     1141 cagtggaaga tacgatcagt attctgcgtg gattaaaaga acgttttgag atccaccatg
     1201 gtgttaacat ccatgacaac gcattagtag cagcagctac tttatctgac cgttatatta
     1261 ctgatcggtt cttaccggac aaagcaatcg atttgatcga tgaagccagt gcaacgattc
     1321 gggtagaaat gaactctatg ccaacggaat tagatcaagt tactagacgt ttgatgcagt
     1381 tggaaatcga agaagctgca ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga
     1441 aaaatttgca agaagaatta gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt
     1501 gggaaacaga aaaagaagaa gtcaattctg tttcagcaaa acgtgctgaa atcgataaag
     1561 caaaacacga attagaagat gcagaaaata actatgattt agaacgcgct gctgtattgc
     1621 gtcatggtac gatacctcaa ttagaaaaag aattgaagga attagaagct aaagcaaaag
     1681 acagcgagat taagatggtt caagagtccg ttaccgaaaa tgagattgct caagtcgttg
     1741 gcagattaac gggtatcccc gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta
     1801 aattgaatga aacacttcat aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca
     1861 gtgacgcggt aattcgttca cgggctggat tgcaagaccc aaatcgtcct ctaggttctt
     1921 tccttttcct aggaccaact ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga
     1981 acttgtttga ctcagaagat catatggtac ggatcgatat gagtgaatat atggaaaaac
     2041 atgctgtgtc tcgtctagtc ggagctcctc caggctatgt aggttacgaa gaaggtggtc
     2101 aattaacgga agctgtcaga cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa
     2161 aagcgcatcc cgatgtcttc aatatcttgt tacaagtcct agatgacgga cgcttgactg
     2221 attcaaaagg gcgagtagta gatttcaaaa atacggttct gatcatgacc agcaatatcg
     2281 gttcacagtt actgctggaa ggcgtgactg ctgatggaac gattccagaa gcagtggctg
     2341 aacaagtcaa cacgctatta cgtggaaact tcaaaccaga attcttgaac cgaatcgacg
     2401 acacgatttt atttacacca ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg
     2461 tggcgcaatt ggcacaacgt ctggaacatc aagaaatcct gctgacaatc agtgatgaag
     2521 ccaaaacgtg gattgctgaa aatgcttatg aaccagcata tggtgcaaga ccattgaaac
     2581 gatttatcac taaggaagta gaaacaccac tagctaaaga aatcgttgca ggacatgtta
     2641 tgcctaaatc aaaagtaacg attactttac ttgatggaca acttcatttc aaaacagaag
     2701 agctagaaga aatcgtctaa tcttccattg agactataaa agaaaaaagg ggtctgacac
     2761 aactagtgtc agacctcctt ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa
     2821 aataagccga aatttcttga agctgcccgc ttctgtcaca acctctttta ttaggtattt
     2881 ttcacttttt ctaatttata actttcaaag taacgctcat cttttttact ttgagaccat
     2941 gcaaacaatc ccattaaggc agcacctatc gtatttgtca gcaagtcgcc catcgtatcc
     3001 atcaatgctg ctcgtccgac aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc
     3061 atgcctaaga actggtcgca taagaattcc cagaactccc aaaacacgcc gcaaagtcca
     3121 gcaaacgcaa agccgaataa gaggaacaac caaggactgg tttttgatat ctcagcatct
     3181 ttcatcaagt aaccaatcaa tccatagcca agagctgtca ggaccattgg actcaccgca
     3241 tgtaagattt tgtcccaaaa agagatgata ctgatcaaat gcatccctgt gcctaaaaag
     3301 acagagatga acaagaaaaa ccagtaaaac aaaacgattg ctgctggtat tttaagtttg
     3361 aaaactttca aaatgacttg tggcatgaaa atcaatgcaa tccctaataa agattccacg
     3421 ataaatgtga ttccttgaga agtcgaatat ttttgtgtga taaattcata aaaattatag
     3481 cagacaacga atacaccaaa agcaagtaaa gctatttgca cctttttgat ttctttaatc
     3541 actgaaagtc cctccatcta aaaatctttt tacttctgaa agcaactcaa tttctgaaga
     3601 aaccactttt ccgttcaatt tgatcaaacc tattgtatat aagttcaggt agtgaaattg
     3661 attttcagct gtttcttgta gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg
     3721 agtatcagta tataagccga tcactgggat gcctttcgca taagccacac cgatttccga
     3781 tgctaccccg gcatcgatcg tcagcccatc tagtaaagca atcattaaat cactagccaa
     3841 cactttttct gtgtcagcaa gagcaatcat tcgactgtct gcataagcag ttttgtcatt
     3901 gattgcttca ttttcttgag gaagatagat cgaaagctca gaagagagct ctctgatttc
     3961 tttaactaac tgttggttat ataacaaatc acttttagca aacattggtg cggcaaaata
     4021 aattttcatc taaacatcat cctttttatc aaaaataatc gctaaagtaa gcttccatgt
     4081 ttggaatgat acgctctagt gacttcagtg tttcttcatc tgtttctaac cgttcgattc
     4141 ttgccaaata agaagcacgg cgataattca tcatcaagca agtcaatgtt tggatatcca
     4201 gtcgaacgga ttttcctata ggttcattta agacacggac ttgtccctgt tcatcccatg
     4261 ttaaagcgaa gacaccatta ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag
     4321 catcagctgt tccaacaaac ggaaaccgtt gaaggaattc ttttacatca acgatccgag
     4381 ccataaaata tggttcgatc tgctctttga tctgactatc ttcaatcaaa aatgctaaag
     4441 gctcattttt ataaatatct ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa
     4501 agttccataa gccatttcga gcttcttgat caagatagaa catttctttt acgtgaaata
     4561 tttcttcagc tacccagtag aacagaacac ctcttggttc atggttgcta tcgtagtaga
     4621 cagcggctgt gcgctcatct tcattttcga aacgccaata ctcttcccaa tgaaaatcac
     4681 tgcggaataa agcaccatgg ttttgttggg caaatttgtc ataaacggaa aatacgtctg
     4741 gatgatcaac tggtaatcgc tcaaccatac cacttactgg gatttgttta ggtagctgtg
     4801 tatctctgat tttaaaggaa agtttatcag acatgatttc ccatccttta cgccgatagt
     4861 aggggatact gtaaggatac aaataagaga tccattgctt attcttgcgc atattatcca
     4921 atgcaacgat aattagatct tgcatcaatc catggttagc atattctgga taagttccaa
     4981 ctccagtcac accacccatt ttataacgtg caccgtgaat attgacttca caaggataga
     5041 tagcgatttg agagatcagt ttattttcgt ggaaccatcc gaacactttc gatacttcca
     5101 aaatcggttt tttggaacgg ataaaagctc gtttattctc aaacccgctt tcctcgatgt
     5161 ctgcttcagt cacttggaac acataggaaa gcaattcatt gaattgatcg atatgttctt
     5221 gagtgaccgg ttttaattca agattttctc taaaatcttt ttgatccatt ttttcatctc
     5281 catttattag cttgataaca gtatagcaaa attctacaaa aaaaacttct atagctgact
     5341 aattaaaaac tctttaaatt actgcttttc gttggtataa tggaaagtgc tgattatatg
     5401 aaaagaggag acgctatgga tataaagaaa atgaaagaac gccaagagca gattcgtaat
     5461 ttttcgatca tcgctcatat cgaccatggg aaatctacat tggccgatcg tattttagaa
     5521 atgacgcaca ctgtgacgtc tcgcgaaatg caagatcagc tgttagactc gatggatttg
     5581 gaacgtgaac gtggaatcac gattaaatta aatgctgtag aactcaatta cacagcgaaa
     5641 aatggagaaa catatatctt tcacttgatt gacaccccag ggcacgtcga cttcacctat
     5701 gaagtctctc gaagccttgc tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa
     5761 ggaatcgaag cacaaacatt ggccaatgtt tatttagcgt tagacaatga tttggagatc
     5821 ctgccagtca tcaataaaat cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa
     5881 atcgaagacg taatcggaat cgatgctagt gaagctgtat tagccagtgc caaatcaggg
     5941 attgggattg aagatattct cgagcagatc gtggaatatg ttcctgcacc aagtggagac
     6001 ttagatgcac cgctaaaagc tttgatcttt gactctattt atgatagtta ccgcggtgtt
     6061 gttctaaatg tccggatcac ggacggtatg gtcaaacccg gagacaaaat caaactgatg
     6121 agtaatggca aaacatttga tgttacagag gtcggggtct tctcgccaaa agcagttgct
     6181 agagactttt tgatggtcgg tgatgtcggt tatatcacag caagtatcaa aactgtccaa
     6241 gatacacgag taggggatac agtaacgtta gcagacaatc ctgccgctga agctttacca
     6301 ggttaccgaa aaatgaatcc gatggtttat tgtggtcttt atccaattga tacttctcgt
     6361 tacaacgatt tgcgggaagc tttagaaaaa ctgcaattga atgatgctgc tttacaattc
     6421 gaaccagaaa cctctcaagc attaggtttt gggttccgct gtggattcct cggacttctt
     6481 catatggatg tcgtccaaga acgcttggaa cgggaattca atctagaatt gatcacgact
     6541 gcaccatccg ttatttacca tgttaataaa acggatggta cgactgctac agtagataac
     6601 ccggcagatt ttcctgaacc tgtaacgatt caggatgtag aagaaccatt cgtcaaagca
     6661 cagatcatgg tcccaaatga ttttgtcggt gcagtaatgg aactttctca acgaaaacgg
     6721 ggagaattca ttacgatgga ttatttagat gattaccgag taaatgtcgt ctataatatt
     6781 ccgctttctg aaattgtctt tgactttttc gacaaattga aatcaagcac gaaaggctat
     6841 gcctcattgg attatgaaat gtctggttat caaaaaagca agttagtgaa gatggacatc
     6901 ttgctgaatg gagaaaaagt cgatgcatta agctttatcg ttcaccgaga ctttgcctat
     6961 gaacgtggaa aagcaatcgt tgaaaaactg aaaaaactaa tcccacgcca acaatttgag
     7021 gtgccaatcc aagcagctat tggacaaaaa atcgttgccc gttcagacat caaagccttg
     7081 cgtaaaaacg tcttggctaa atgttatggt ggagatgttt ctcgtaaacg taaactctta
     7141 gagaaacaaa aagaagggaa gaagcggatg aaacaaatcg gctccgtcga agttccgcaa
     7201 gaagccttca tggccgttct gaaaatggat gagcaagata gtgtgaaata acttataatc
     7261 aatcgaaaaa cctattaaat aaaccttttt gagtagatgc aacactgagg attatctgta
     7321 taaaagattt taaactgtca tttggttatc acggcgatac taaaattaac aaaattggaa
     7381 tatttttctt gaagattttt agctgtcagt atatgatgaa agcatctcat aggaggtgct
     7441 tttttcatgg ttgataattc aagtggaagg gttcttaaat ctctaagaaa agagaaaaaa
     7501 ctatctcaaa aaaagttagc tgatttagca ggtatttcac aaagtacttt agttaaatat
     7561 gaaaaagggt caagaaaaat ccctaaagac gtagataata ctctaagcaa gattctgaat
     7621 atagaaactc taataaaaga tgaggaagat aagattgaga ttctcatcgg taaactaatt
     7681 gcatacagag
//
LOCUS       DAFGFZ010000126         7644 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000126 DAFGFZ010000000
VERSION     DAFGFZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.128"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(75..410)
                     /locus_tag="LR726_002499"
     CDS             complement(75..410)
                     /locus_tag="LR726_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217538.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(585..1079)
                     /locus_tag="LR726_002500"
     CDS             complement(585..1079)
                     /locus_tag="LR726_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217539.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFEL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            complement(1076..1303)
                     /locus_tag="LR726_002501"
     CDS             complement(1076..1303)
                     /locus_tag="LR726_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217540.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(1470..3440)
                     /locus_tag="LR726_002502"
     CDS             complement(1470..3440)
                     /locus_tag="LR726_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217541.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRLEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAEINVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(3455..4321)
                     /locus_tag="LR726_002503"
     CDS             complement(3455..4321)
                     /locus_tag="LR726_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217542.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(4311..4544)
                     /locus_tag="LR726_002504"
     CDS             complement(4311..4544)
                     /locus_tag="LR726_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL6217543.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(4556..6712)
                     /locus_tag="LR726_002505"
     CDS             complement(4556..6712)
                     /locus_tag="LR726_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217544.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAEDVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEDATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(6732..6953)
                     /locus_tag="LR726_002506"
     CDS             complement(6732..6953)
                     /locus_tag="LR726_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217545.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(7063..7401)
                     /locus_tag="LR726_002507"
     CDS             complement(7063..7401)
                     /locus_tag="LR726_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217546.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
BASE COUNT         2189 a         1494 c         1030 g         2931 t
ORIGIN      
        1 tgaaatggcg gaaaacattt cattgaaaaa aggattgaac ataatgttca atcctttttt
       61 atagttataa tcaattaaca ttcggtgtgt gaaattgaat cttcgatgct ttcacatgga
      121 aaattattga ttccttcttc actcatttcc catgtagtat agaaaggagt taactgtcct
      181 ccatactcct gaataataaa atattttcca tcattgatat ggttacaaca gtacactcca
      241 atagttggat actcgactac taaaaattgg tctggatcaa actgttcttt tttcaacatt
      301 tctcgtattt gaaacaaatg atccttctct aagcaatcag gtaattcctt tattctttgc
      361 gttactaatg acccctctat cgctttccat tcatttaaat attgtttcat aacaattcat
      421 tccttttatg ttaactattt ttcctaggaa attgttttaa aacgccactc tcccagaaaa
      481 cgtaagattt ggttagctag aatatacctt ccagctaacc acaattttcc attactatat
      541 tgtaagcttg ctttcacttc ataaaaattg ctgatatact cttttcaaat cgaactcttc
      601 ctatctgaga agggtatttc ttgcgctagc ccctcataag ttccatcttc attttcccaa
      661 atttctacct gaaatactga attattggct aatttttgtt gcaagagata atagggttct
      721 tcttcatact tttgatcgac ttctttaaga cattcaatag catcaatatc aatattttga
      781 aagcccaatt tttctagata acaatcgtac caagtacccc atgtattatc cagaaatgag
      841 acaaatgcat gatccatatc atttgctata aaagattcat cttcccacct gaagaggtaa
      901 tcccactcac cgtttgcaat tttagttctg atttcatttt ctttttttaa ctcaaaagga
      961 ttacaccaac aatgctcatg tcgcatccaa acaaaaaatt gtccattctc atctatttta
     1021 aattggctga gatcaccttt caatctaaat acacggttct tttctggtaa cttattcatg
     1081 cttcaaccac ctcatactgt tctctcatac ctttgacttc attaaaaaaa actctcgctt
     1141 tttcttcatc agtaaagttt ttatacaact gattacaata atgaggtttt tgaagtttct
     1201 gaatacctat actaacttca aatccttctt catgttgaat taggattact tctgtgtctc
     1261 tacctagaaa tattcgatct aaaattttta tgtttggttt cattatttaa cctccgctat
     1321 aattatttca ctcagtaagg tttcgcttta tgaaatttct atagttctct tttcacaaaa
     1381 cgtaccatgt tctctaagac tgtttttagt ctcaaatata catgtttttt cttatccttt
     1441 ccttatacac tcaaaatcaa agataaaact taaagaataa actctccaat aacaaaatta
     1501 ttagtcagat ccactggtct cctgatctga aatttattac ttcttcctgt tgtcaaatat
     1561 tgtctcaaat caacagaaag tttttgatca tcttctcctt ttctagcagg agcaactttt
     1621 ttttcatact tcattaactc tccactggtc atctttccaa tgatagtgta attaaaatca
     1681 ctttcaaaac tttggtaaat ttttccatct gtattgaata tatcagctgt ttcaatatct
     1741 tctttgtatt cattgtcttc tcctgaaccg attgtatcaa atagaaaggc aagacgaatt
     1801 ccatcttctt caagcattgt taatcttttt ttaattagcc caacggtttc aatagataac
     1861 ttatcgaagc cgtgaatcat aacaatatca tctttttttg cttgtgatgt gatgaaatca
     1921 aaggtattta aaaattgagc ttccataaca ttgatttctg ctcttagatc acctaagtta
     1981 tagtaaaact gtaatttgtc acttttaccg atttctttta gtgtcgttct ttgattaaac
     2041 atcctagaga agctgtctaa tgctgttctc aatgtgtctt ttaatacaat aatacgatcg
     2101 acttcttttt cagtaccaga ttttttggct tttgtttcag cattagtcaa atagctaata
     2161 aaatccccaa gaactggcat tgtatcactg ctgttcacca aaagttttga acgttcttca
     2221 taattaatat cccaaagttt ctgacttata taaaagttac ctaattcttt tttcaataaa
     2281 actctagttg cagaatctaa tgttctgcct gctaacaaat aaaaaatctg agttagttta
     2341 tcgagattcc gtctatataa atcaccaatg tccttttgat taccaaaaag ctccaaagag
     2401 ttaagacctt cttcagccaa attaaccttt tctattacac tgtttaacga aggtttggca
     2461 acaaatgttc taccagttgg atcaccaaag taatcaaatt tatttaagac aatatgaaaa
     2521 gttcgtttat gatttgccat tgcattatta gcaatcaatt gcccccaaag tgatgatgct
     2581 gagatttttt catcatagcc tttaattgta gcgtttttgt gtgatgccac taaaacttta
     2641 cttgtaaaag aatcatccag tgccataaaa gctacaccac ttgcgtagct agtagacata
     2701 gtgccgatag cccatccatt cgcatcatct aaacctttac gaattgcatg atcatttcct
     2761 gcaaaatccg aactcataga cgtatactca tagacattcc cgattggttc acttaaaagt
     2821 ctatcaaaca gatcacgttg atctcctcca tatgacataa gctttaatcc atacattttt
     2881 tcatcataca accgttggag tttaattagc tgttcgctga ttttattagg agaatcggat
     2941 actaatagga tttctagctt ccaatcaata attttatttg ccatgctgtc tagtaaagat
     3001 gctgtattca aatccataat ccgcataaca gttttgctaa tttcagcatc tgaatttttt
     3061 ccttctaaat tattttcata actattcgtt gatcccccaa cattctcgtg cattatttct
     3121 tgttgatctt tttgtgacac tggcttatct gcttcaatta gataggcttt taccccttct
     3181 agtctcactt caggaatagc tttaaccatc caaccaaatc cataatttct attcattcca
     3241 taagtattca ctaatttcac aattgttccc caccgcttac ctgtcctaat gtaagatggt
     3301 gtaaattgat aaccaactaa agatgttaaa tcactaacaa aggtattatt cactaccttt
     3361 gaatttctag ttttttgttc ttttttagtt tttctctttt ttttgaaatt taatagttgt
     3421 gatctaccca taatttccat ttaatctctc acctctattt cagtgcataa atatcagata
     3481 atatttcttt tgtttgttta gctgacaact gagtgacact ataataaagt cctctactcg
     3541 tatagttttc taaacgttcc acaccttcat ccaataccat cttagattga gatttcaaga
     3601 taagatattg gattactgtt gttttcacac catcaatata ttctgataca aatcgttctt
     3661 gctgatctat tatgtcaaag actgctttgt tactccttgc atttcgtttt tgtattttca
     3721 tatttcgagt ttgtcgttcc gtattctgag gttgagatga agtgattttg atttcagcta
     3781 aggaacgatc tctggtattt tgatattgta ttgatttcga ttcctgagcc ataacttctt
     3841 ctggaaaagc ggttagtgat gtagccccat ctagattgaa cattaatgct acactcccat
     3901 ctcgatatac gattgttcca tcttgtttca cttttaaaat ctctgtgcct cgaaatggtt
     3961 tctttttacc catagatact cttgctgaat taactactga tttgaacagt ctaatagaac
     4021 cagaaatcaa tgaaccacta gatggatcaa taaacatata ctggctaatt agaataagaa
     4081 atccaattat cattaataac gttggaacaa aaataaattt cagtttaatc gtatcagaaa
     4141 ctaagtaaac aattgatcca agaacaacga ctgcaacaac tatagctgat acaattaatc
     4201 tttttgctgt aacagaatag gtgctattga catgcttttc aagccattca actctgacaa
     4261 ttgtttcaga aggtttttga tgtaagttat gtttaaatcc atagtttgag ttatctgcca
     4321 tcagaaatac ttcctttcac gttcaactta cggtaaacac ctacagttat cactattgca
     4381 acaataagca agccaattaa agatattgac ttgctcattg tctcgttcgt ttgtggtagt
     4441 tcatttacct gatacacgag ctgtgcttcc tgtaagtgct gagaagaggt catgttttct
     4501 tgattgtttt gaattactgg ataaactatt ttttcttctc gcataatagc cctccttagt
     4561 tattatcaga taagtgagct tgaattttgt actgatttcc gtctggagtt ttcacaactg
     4621 ctttttctcc atcaatagaa atcacttcag ccttagttgt tttcccatca atactgatga
     4681 caacatcttt cccaactttg aactctgtag ttggtgttgc ttcatagcta ctatttgttg
     4741 aagcagagtt cgtatttgtc ccaatcgaat tttccactct cgaaatagat gtttgagttt
     4801 gagcctgtgc ttgttgatta ttatgactaa taaagtagga cgatcccaag atcgttccgc
     4861 aagccattgc agcgacagca atgatagtag ccatcttttt acttgtaaaa tcacgttttg
     4921 aacttgattg attttctgta gcaactgcta atgatttcgt tttttctgcc atttgagcta
     4981 gatcacttcg ggaacgagat aagtcttctc ttaaagcttc actttgttct gccaactttt
     5041 cgctatttaa tttataaccc tctgctaaat ctttgacctc tttcattcga tctttatatt
     5101 cttcgatagt tttttgttgc tctacaagtt ttgtttctaa ttctttcgtg ttgttagcta
     5161 cctgcttttc aacttcttct ttgttcgcaa aatatttgac aaccacatct gttgttttct
     5221 cttttgtatc atggaactca gcttcatttt ctgactttgt tttttcgaca atctctaaag
     5281 ttttttcttg cacgtctgtt aatgattttt caatttctgg tttcaatgaa ttgataattc
     5341 gttccaccac aacttctttt gcttcttggc tttgtttcaa tgcatcgttt ttaatagatt
     5401 caattgcttc ttggtactgt tgatcaagac tttcttcagt tgcttttcta tattcatctt
     5461 cttgttgttt aacttctggt aaatgttcag catcataccg ttgctctagt ttctttagtt
     5521 cagcatccaa ataagcctgt ttcgccatat cataatcttt acgaatgata ttggattttt
     5581 ccatcactaa atcatcaatt ttagatgctt ctgcttcgta gctctcttta gcaaccgata
     5641 gtgtatttgt aaactgattt tgaccattta atgaggttgc atcctctatc cgcaaaagct
     5701 catcggctaa catttgcttt cctttgagga tcaattgctc cttcgtctta tttaattcac
     5761 tgttgatttt attattagca atagagatac gagcgttcat actctcttta gctaaaaaca
     5821 gcaatggttt caactcaggt atggatagca tctcctcgtt taaatcgaca gggtcattaa
     5881 tctcatctaa aactacaggt tgaaatttgt cagcaatgat ctgttcaaaa tttttttcta
     5941 aaaaatcagc atctaagttt ttcggtacat cactttcaga tgtgattttt tcagcttcat
     6001 aaacataatt ttctttgtca ctttgggatt cgggtttttc atcaattttt ggttgttggt
     6061 aggtttctgt ttgtatttgt gtttcttcta tgcttacttc ttggttttct gcttcggttg
     6121 gtttgttatc atccacaaag agagcttctt tttcatctgg tacatcttct gctacggctt
     6181 caaattcaac ttcttcagaa gagttgaagg tctcagcaat tcgttctaat ccattagtag
     6241 ctacttctaa ttgctctact gcgctataga agtctgtagc ttcttcaact tctgattcaa
     6301 acacattagg atcatccgga ataactaata attgaccact actatccata aaacggacac
     6361 ctgctaatag cttctcttta aatttttctg gtagatcagc tacataattc aaacttgact
     6421 tcaatagaac gacatacttt tcagaatcga gaatagcatc atcctcatct ataactccct
     6481 cactgttgag cttttgtaca tcttcaatta ccatataatt tttctttggt aaatctttgg
     6541 tcagcaacgc atcgaattct ttccatacct ttgctttagc tttttttgcc ttcgcttctt
     6601 tttcttcttt tttgtacact tcgagtaatt gtggcttcaa agagagtgca actacaggaa
     6661 ctgtttctgc cacataatta atagattcgg taatatcttt gaattttgcc attattgtaa
     6721 aactcctttt ttcagtcaat agttcttttc tgtttgtcta gcatatatac atatgctttt
     6781 tcgaatttaa taagcgtttc aagtctggct ttttcaatct ttttactccc atttcgataa
     6841 tcatagaact ggttaacatt catattcatt atctttgcca gttcggtacc tttcagactg
     6901 ctcattaata cttcgttggc gtgagctaac attttttctc tatcaaaaac catcttacat
     6961 ttactttctt ccctcacttc taaactacgc ttggagcgta gttttttggt aaaaagtaaa
     7021 ttgaacaaat cggcatatta tccaaatttg ttcaattcac ttttaaccta actgttcatt
     7081 ttttggaaat tttgaaacta gatagatacc aacaactgga ataagtgctg atatgatatt
     7141 agctttttgg ttcaaatcat tacctgtttt agtacccatt gtgtaaagca taacggttgt
     7201 aattacagga ataataaaga ttgcaataat cgccccaaca tattttaatc catctcccca
     7261 ttttgcatca ccaaaaaatt tcattgcagt gaatcctggt ttcaacgaaa aaacgaatgc
     7321 caagaaatac aaaacaactt gtactaaagt tttcatatca ggcgtcattc cgctaaccaa
     7381 atttttaata gaatcgaaca tataaaaacc tcctttttaa tatttttaat aagttaatta
     7441 tatcatcgac aaatcttatt tgttcacttg tcaatgatct aactaagaaa aagacgtctc
     7501 gatatttaca tctttatgtt atcataataa cataaatttt cgatgattaa aagagatttt
     7561 ccattaaatt atttcacttt ttttattggc taaaaatgct ggtttatcaa gtttttgttt
     7621 agataataat tctgttcttt acca
//
LOCUS       DAFGFZ010000127         7630 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000127 DAFGFZ010000000
VERSION     DAFGFZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.129"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            18..2345
                     /locus_tag="LR726_002508"
     CDS             18..2345
                     /locus_tag="LR726_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL6217547.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2471..2800
                     /locus_tag="LR726_002509"
     CDS             2471..2800
                     /locus_tag="LR726_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL6217548.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2840..3844
                     /locus_tag="LR726_002510"
     CDS             2840..3844
                     /locus_tag="LR726_002510"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL6217549.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3841..4287
                     /locus_tag="LR726_002511"
     CDS             3841..4287
                     /locus_tag="LR726_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL6217550.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4437..5252
                     /locus_tag="LR726_002512"
     CDS             4437..5252
                     /locus_tag="LR726_002512"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL6217551.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5458..6630
                     /locus_tag="LR726_002513"
     CDS             5458..6630
                     /locus_tag="LR726_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL6217552.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6792..7286)
                     /locus_tag="LR726_002514"
     CDS             complement(6792..7286)
                     /locus_tag="LR726_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217553.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7283..7477)
                     /locus_tag="LR726_002515"
     CDS             complement(7283..7477)
                     /locus_tag="LR726_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217554.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2570 a         1443 c         1520 g         2097 t
ORIGIN      
        1 ttttattgag aggagggatg aaaatgatta agaaaactca aaataaaatc caacgtatat
       61 gtcaaaaaca ttttgtttct agactcaaag ctgtatctaa aagaaaatac ttacatccca
      121 ttatgatttc gatcatcttg atttttactt tcttagggag cttatcaagt atttatgtaa
      181 acgtttatac agaaacgtca aattttgcta accaaacgac agaggagaca aatagtccac
      241 cctattctga aatacagtcc atagaaagca ccaatcagtc tagcaacaac gagacaacct
      301 caaccacatc ctcgactcaa ctgattgatc cgacagaaaa agaaatcaca gcagcaaaag
      361 cagcagggga gaaagcttat tcagaaacag gtagaagcca gacatttagt actgttgcgg
      421 ccactgcgaa tttcaacgga cataccatac cagatggata tacagtgata tatgccaaag
      481 atggagtcat tgttgcggtt aacggatcga tgagtgcgga taaaactgcg ttacaaggcg
      541 taaccaaagc aatcgctttt attgataacc gtccagatat ccataatctg tttattggct
      601 ccacagtaaa tggtactact atcacattcc caaaaagtat ctatatgggg gtggctaccg
      661 ctggaaatac gatcgattca gttacaccca atgcggataa aagttattct tttacaccct
      721 caacatgggg agggcttcct gccggaggag gtatgagtac tttttcaatc ggtatacagt
      781 tagcaaatga tattccattc acggggtact cgatcaaaaa tgggcagatc gttgatggta
      841 agggaaatgt ttcaaatttt ccaacctatc aagcagcaac ggttcaggtc aatgcgaatc
      901 ggacaggaac tttttcttat acagtcaatg gtggagcagc aaaaatcgga acgtacggga
      961 ctacattatc tggaatccat gtgggtgaca aagtggttat cactccaaca gcagtaacgg
     1021 gctattcttt tgcacaaaca aacggttctt tcacagccct agaaagtaac gtcgatacgg
     1081 tgacctatac aggcaaacaa acaccaatca ctattaagta tcaagactat tttggacaga
     1141 tgattgctcc ttctaaaata atgaatctta cttacggaag tgctgcacaa gacctgacga
     1201 ctaatacgcc tactatcaac ggatatacct ttacagctgt ttcagcgata gaaacaaaga
     1261 accaatcagc aatgagtgtt ttagctagtt tagatacaaa tggaaatgta atcgtaaagg
     1321 acgcaaatgg aaaacagatt tctgaagtca tcctatatta taagacaaat gcaacagtat
     1381 caatcaatgc aaatggcagt aagtactatg atggtctttc tgttgtaccg atcgttaaat
     1441 atgcctttaa tgataaaaca gaatcaacat cccttttaaa caatgatctc agtattgatc
     1501 aaatcacatg gaatccaact gatttcaaag cgatgaatga aaagggagtt gaagtaactg
     1561 cgcctactga aattggaacc tatacgtctt ggcaattgac aaaaagcgga ctagctaaac
     1621 tagctgccag aacaaactat ttatttacta tcgtgcagac atctgatgtc tatacgatta
     1681 agcaaatcag cggtgcggta gagctcagtg gcagtaagac atatgatgga aaagcaggtg
     1741 tcccgtcaat ccatgtcaag ctagcagagg gtgtcactag ttcgcttact ccagtacaag
     1801 tggcacttag cagtatggat tacacagcag atgcacaatc agcaaaaaat atggttaatg
     1861 ctggcagcta tacaattaaa ctaacgaata gcggaataga caaagtcaaa gcagcaaatc
     1921 ccaagttgtc atttactgat atagccaaca catcaggaac ttataccatc tataaagcta
     1981 acgcagtgat tactgtcgat gacgctgcct ttaattatga tgcccaaagt cactcgattt
     2041 cggttggaaa tctccacgtt accggtgttg tcttaggcga atcattggat tataccctaa
     2101 cagttaacag ccggacagat attggatcac aaatagtaac gattagttta ggcgaaggaa
     2161 gtgtaaacaa taattatacg attatcgcaa cagataccgc taagttgacg atcaatccga
     2221 ctctcttgct tccttcgaca ggtgggatag gtctactgcc ctttgtcttt ttaggactga
     2281 tctttatttt ttctggcttc ttttatttta tccatcgtaa gaaaaaagca ggtgaacagc
     2341 gatgaaaaaa acaaaaaatg tacgtgtgga tatatttgct gttctctttt ttatcctact
     2401 ggtctttttt ataggttctc cgactgtgtc agctagagaa tcacctatta catttacttt
     2461 gcataatcga atgttcgaaa gcgataagat gccaagtatc agtcaaaacg acggaataaa
     2521 tatcccggat aactttcaag gagtaaaagg gttgaacggc gttactttcg ctatatatga
     2581 tgtctcggat gaattttaca agctgcggtc agaaggaagt agtgtggaag acgcacaaag
     2641 aaaactagca caaaaaagtg atagtgaaaa aatactggca gaaaatgtga caaaaacagt
     2701 agacgaggaa gagggcattg cttcttttag tgcatcggac aaagatgagc aaggtcgtga
     2761 cgctgtttac cgatttacgg aaataaagac ttcagattaa gtaaaagaaa aatcagcacc
     2821 atttgtcgtt gtgcttcctg tgacagattc atctaatcat aaattgacga ccatccactt
     2881 gtatccaaag agcgaacaaa aaatacctgc tgcactcaca ttaacaaaaa cagtagagaa
     2941 taagcagaca gattttgcag atggggataa agtcccttat ctcatcaccg taacaattcc
     3001 tgagaacatc aacgagatag gaacctatac gatcaaagat acagctgatc ctcagttatg
     3061 gctagaacca gaaacgatcg attttttgat tggtggagac aagattcata catttcacac
     3121 acaaaaaata aaacatggtt ttgtcttatc agattctgga aaagatctta gttcgtttgc
     3181 tggaaaaaaa ttaacgatcc gttaccaaat gacattgaaa gaaaacagtg aaaaaacgac
     3241 atttgataac gatgtacgtt tgacgactga caatcaaaca gtagaaacac atcttgccat
     3301 ccaaacagga ggcaaacaat ttgtcaaagt ggatcggcaa acgaaacaaa aactagcaga
     3361 tgctcaattt ctagtcaaaa aaaatgacca atacttagcg caagaaaacg ggataaatca
     3421 ttgggtagcc aaagatgatt caagagcaac gatttttact tccagtaaag atggaacatt
     3481 ttctgtccat ggattgtctt ttggaagtta cgaactagtg gagattaaac cgcctaaagg
     3541 atatatattg agtcaatcaa ccattctctt tgaagttgaa aaaggcagtt atgatccgct
     3601 tcatcctata ccattggaaa tcatcaacga accaaaagca aatccgacga ataatggaaa
     3661 aacgccagtt cagatgcaga caaatggtcc atcaaatgaa aaaaatggag gaagctttcc
     3721 aaaaacaagc gaagagatgc ttggaggttt ctcaatttta gggttattgc tagtcctttc
     3781 aacagggaca gcttggtttt ataaaaaaca acaaaaagga aacaatagga gggaaataaa
     3841 atgaaaaaca tcaaaacaaa aatatggacg tttttaggaa ctgctatcat gctgcttcct
     3901 ttcgttctcg gcctaggaac tgcagaggtt tctgcagccg tatcacctac accagaaaat
     3961 gttacggtta atttgcataa attgaaattc acttcagcac cagaaaatca aataaacaac
     4021 ggaacggaat tgacatttcc gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat
     4081 gacatcactg ctacgtacta tccaagtaaa gacacagcag ttccagcaga cgctacaccc
     4141 ttcgcaagtg taacgacttc tggtgaagga ttagctaacc tgactcttcc aggtaaatct
     4201 gatgggaaag atgctgtata cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg
     4261 ccaaatatcg tgttaagctt accctagacc ctgacaaacc tttagacacg attcacctat
     4321 atgctaaaaa tgtcatcaaa gaaagcaata tcaaaatcca gaaagctggg aacttttcag
     4381 gggtagataa gttagcaggg gcgcaattcc agatcaaaaa ttcagagaat caattcgtga
     4441 ccggttttga tgaaaacact ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat
     4501 tcacaactga tactgaagga tccatcagtg tcaatggatt aatagacggc aattatactt
     4561 tagttgaaac aaaagcacca gatggttatc aattaaccaa tccacaaaaa gaaactactt
     4621 ttatagtaaa aaacggaaaa ttgcaacaaa caaccggtat catccatggc gcagatgaag
     4681 ccaatcttgg gaatccaaac ggtgtagtca atcgggaaaa tacaactacg gataacacga
     4741 ttactgtaga aaatgtccaa aactacggtg atttccactt catcaaacaa gatgtgaaca
     4801 cgcaagaaaa attagccgat gcagaatttt ctattctaag agcaaaagat ccaactgtta
     4861 aattaatgaa aaaaatagca aaccttgcct caggagaata cccatatgct tggtcagatg
     4921 aagtcacagg ttctgactgg gaagaagtga aattgatttc tgattcagaa gggaaatttt
     4981 caatcgaagg gctgaaacaa ggggcatact gccttcaaga aacaaaagca ccagaaggct
     5041 acgcattacc taagaatgaa gctgcgtaca ctgaatttac agtagatgat gatccaacaa
     5101 cttctgatga aacaacgatc gataaccaac ctaaaggaat cttgccttca actggtggta
     5161 tggggatcat cgtattcatc ttggtaggta ctgctttagt tggaggagca gtgatctatt
     5221 tcaaaaaacg ccatcaagaa acagaagctt aaactattgg aaacaaaaat catacgataa
     5281 ataaacgata gtgattcaaa tggggagaaa ggttgtgaca agtggtcggt ctcttggtat
     5341 tcgctcaaca aacggcaaaa taggaggaac tattttgccg tttgcttcgc taatactcag
     5401 gtcaaggcgc tgatttcaca acctcttcat atacatatta gaaaataggt ggacatgatg
     5461 aataaaagca gaataagaat actggacatc ttcatggcaa tcattttagt cgttgggatc
     5521 gggatctttt cttatccttt tgtagaagat tcactgaatg attttcttgc ccagcaaatg
     5581 atcatccatt atcaaaagca agcatcgaat aagaattcag aggaaataaa aaaacagcag
     5641 gaaaaaatga caaagaaaaa ccaacagttg gcagagcaaa acgtatcacc agggataggc
     5701 agtttcaacc aagcagttga tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt
     5761 atggaacata tgcttggagt catcgaaatc ccaaaaatca atgtcagctt acctatattc
     5821 gatcagacaa cggaaatatt tttgcaaaaa ggaacgtcat tattggaaga aagttcttat
     5881 ccgactggcg ggaaaaacac ccatgccgtt ctttctgggc atcgtggact cccagaagca
     5941 aaattattta cagatttgcc taagttgaaa aaaggagacc agtttttcat ccagatcaat
     6001 ggaaagacat tggcgtatca agtagaaaaa atccaagtgg tcttacctga tgaagtagat
     6061 tctttgggta tccaaaaagg cagagatctc gtgactttgc ttacctgtac tccttatatg
     6121 gtcaatacac atcgactgct tgtgacgggg catcgtattc cttatcaagc aaaagaggca
     6181 aaaaaagcga tccaaggaat tgatcaatgg aaaaaatgga aattttttat ttggtttatc
     6241 ggtatactac ttggatcaat cggcttagtt tggctactca tcgcttattt agattctttg
     6301 gccatcgcta agcgaaacta tcctttatct ttttatgtaa agaacacaaa cggacgacca
     6361 atcgaaggaa tggtcttttc tgtaaaaacg ctcaacggca aacactatat tactagagaa
     6421 aaagttccat ttgtgaaagc ttctgatgaa tacggactag tgagattttc tgatttaaaa
     6481 ggcaggaact atcgcttgca acatgaggag ctcctgctta aaatccatgt aaaacataaa
     6541 cattcgaaac agttttcaat gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa
     6601 gaggtgtact atttgattga gaaagaatga atgaagatgt ccaaaaaagc agagggaaca
     6661 gactaaatga aaaaagagag aagctgacga aatggacgtg taaactgtta tttttggttt
     6721 actatctgga acgccatctt ctattttttt atgtttcaga agaattttat tattatagta
     6781 taacttttga attagtttcc ataacatttg gagcaagggg ttaaaccacg agcttgagct
     6841 tcgcttaaag tggcaggggt atacgtacca tttccacatt tatgtgtatg gtattttgaa
     6901 ccggtcggtg tgacatagac agtttgttca ttattttgct caggagctgc ggcttgtgcc
     6961 tgctgagagg cctgttcagc tgctgattgc tgtcgcgcct gttctgcttg ttgctgtgcg
     7021 attgcttctt gcctcgcctg ttctgcttgt tgctgtgcga ttgcttcttg ttgcgcttgt
     7081 tctgcttgtt gtcgtgcaat ttcttcttgc tttttccgtt cttcttctgc ttcttttgct
     7141 ttgattgtgg aatcaattgc agcaagacgg cttgtcaact cttgtttgtt ttcaggtagc
     7201 gcttggatag ctgctgaagc agttgcatag ttttcttttg taggatttgc ttcagcttgt
     7261 gacaaagcag attccgcttc tgtcactttt tttctagtgc agctttttcc gctttttgtt
     7321 tctcttcttt tttcttttca atttgttctt gttctttcga tgaatctgcc gtactttcgg
     7381 agatcgtggt actgctgctg gaagcctgat ctgaacttgt gctttgagta gtagacgtag
     7441 tagcaccaca cccactcaac aaacttcctg cgaccaaaaa caatgcaact aattttttcg
     7501 ctaaatttcc tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt
     7561 acttacttgt tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg
     7621 tgttgataca
//
LOCUS       DAFGFZ010000128         7616 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000128 DAFGFZ010000000
VERSION     DAFGFZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.130"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            69..1496
                     /locus_tag="LR726_002516"
     CDS             69..1496
                     /locus_tag="LR726_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217555.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="LR726_002517"
     CDS             complement(1588..1953)
                     /locus_tag="LR726_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL6217556.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1950..2315)
                     /locus_tag="LR726_002518"
     CDS             complement(1950..2315)
                     /locus_tag="LR726_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL6217557.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2507..2755)
                     /locus_tag="LR726_002519"
     CDS             complement(2507..2755)
                     /locus_tag="LR726_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217558.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2754..2882
                     /locus_tag="LR726_002520"
     CDS             2754..2882
                     /locus_tag="LR726_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL6217559.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2939..4633
                     /locus_tag="LR726_002521"
     CDS             2939..4633
                     /locus_tag="LR726_002521"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL6217560.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4637..4756
                     /locus_tag="LR726_002522"
                     /pseudo
     CDS             4637..4756
                     /locus_tag="LR726_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5284..6417
                     /locus_tag="LR726_002523"
     CDS             5284..6417
                     /locus_tag="LR726_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL6217561.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6448..6768
                     /locus_tag="LR726_002524"
     CDS             6448..6768
                     /locus_tag="LR726_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL6217562.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6797..7261)
                     /locus_tag="LR726_002525"
     CDS             complement(6797..7261)
                     /locus_tag="LR726_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217563.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7393..>7616)
                     /locus_tag="LR726_002526"
     CDS             complement(7393..>7616)
                     /locus_tag="LR726_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217564.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2493 a         1468 c         1371 g         2284 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2341 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2401 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2461 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2521 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2581 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2641 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2701 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2761 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2821 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2881 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2941 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3001 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3061 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3121 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3181 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3241 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3301 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3361 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3421 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3481 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3541 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3601 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3661 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3721 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3781 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3841 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3901 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3961 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4021 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4081 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca
     4141 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4201 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4261 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4321 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4381 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4441 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4501 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4561 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4621 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4681 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4741 ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc
     4801 agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca
     4861 agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag
     4921 aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa
     4981 ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat
     5041 tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat
     5101 gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta
     5161 tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa
     5221 aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct
     5281 ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat
     5341 ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc
     5401 tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga
     5461 cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg
     5521 caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac
     5581 atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt
     5641 ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca
     5701 gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta
     5761 tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat
     5821 acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg
     5881 gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct
     5941 aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg
     6001 tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta
     6061 gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca
     6121 ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat
     6181 atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag
     6241 catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga
     6301 ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca
     6361 tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt
     6421 cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc
     6481 gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa
     6541 aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt
     6601 ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct
     6661 ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac
     6721 gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa
     6781 tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac
     6841 tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat
     6901 ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta
     6961 atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat
     7021 ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga
     7081 tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc
     7141 cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat
     7201 tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca
     7261 atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa
     7321 ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg
     7381 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7441 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7501 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7561 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taacta
//
LOCUS       DAFGFZ010000129         7608 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000129 DAFGFZ010000000
VERSION     DAFGFZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.131"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            180..497
                     /locus_tag="LR726_002527"
     CDS             180..497
                     /locus_tag="LR726_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217565.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            430..909
                     /locus_tag="LR726_002528"
     CDS             430..909
                     /locus_tag="LR726_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBL6217566.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            926..1102
                     /locus_tag="LR726_002529"
     CDS             926..1102
                     /locus_tag="LR726_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217567.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1359..1790
                     /locus_tag="LR726_002530"
     CDS             1359..1790
                     /locus_tag="LR726_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217568.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            1796..2113
                     /locus_tag="LR726_002531"
     CDS             1796..2113
                     /locus_tag="LR726_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBL6217569.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2232..2918
                     /locus_tag="LR726_002532"
     CDS             2232..2918
                     /locus_tag="LR726_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL6217570.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            2893..4242
                     /locus_tag="LR726_002533"
     CDS             2893..4242
                     /locus_tag="LR726_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL6217571.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4233..4706
                     /locus_tag="LR726_002534"
     CDS             4233..4706
                     /locus_tag="LR726_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL6217572.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            4681..5208
                     /locus_tag="LR726_002535"
     CDS             4681..5208
                     /locus_tag="LR726_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBL6217573.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            5223..7535
                     /locus_tag="LR726_002536"
     CDS             5223..7535
                     /locus_tag="LR726_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL6217574.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         2700 a         1298 c         1702 g         1908 t
ORIGIN      
        1 aatttttatc aagtaattca ccaccttttt actcaaatta tatcaaaaag gagagaagaa
       61 ataatgcaag aattagtaat tttgaaaaat aaaaaaagac acgaacgccg gcaagcatga
      121 tcgtgtcagg gaaataaact atctttctat attttaccac agaaaggaat gaaccgtaaa
      181 tgctaattgc aacggataca ctagaccgca tctttttaaa agacgaatac aaactgcgca
      241 atatagatgc gtcaggaatt ttagtatttg atctttatga caatggaaaa attggtatct
      301 atcaagcaag tgatattgaa gaaacaaacc ttgctttcga acaaattgat gattctgtgg
      361 aattggattt agatgaggca atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg
      421 cacaaaagaa tggcaactct ttaccaactc agcgagtcat atatcaaagt cctggaactg
      481 gcagaacaat tggatgaaga aattcttcgc gatactctcg attcgattaa tgaagcgatc
      541 gagtataagg cagaaaactt agcaaaaata gttaaagaag tagaagggaa agctgagtta
      601 atcgatagtg aaattaaacg tctgcaggaa cgtaagacat cgcttttgaa caatgctaag
      661 agtatcaagc actatttaca agaggaaatg gaaaagactg gtaaaacgaa gattaaaggt
      721 gaattattca acattggaat tcaaaataat ccagtatcgg tcaatgtaat caatgagaac
      781 ttaattccaa aaggattctt tacccctgtg cctcccaaat tggataaaaa gcaattgaag
      841 gaggaactga agcacggaga tattcctggt gctgaactcg tccaaacaaa aggtttgagg
      901 ataagatgat gaaggaggag aaataatgga acacgaaaac gtaaaagacg ctttgaaagc
      961 tgctatcgaa atcgcggaag ctaaaggaat aaaggttgac ggcaaaccag caactgtcca
     1021 tgacattcaa aatttgacga aagaacatct ttattttatc gccgacttgc ttggtttgtc
     1081 ggaattgtat ttagacaaat aaccccacac ggaaggagtg gctagggtgg ttgtattaca
     1141 agtaatagtc tgcgtaacat tcatttggtc gtgcataccg gtttgtatat atatcatgaa
     1201 gaagcttcgc cctgagtctt acaaattata cgcaatttac agcataacag tagctcttgc
     1261 atttacattg tttgcaattt ctaggcttcc gttttaatcg tgggaatgaa tttttaatga
     1321 actaaattat ctataaagca aaaaaaggag aaacaacatt gaaaaacaaa ctagcaaaaa
     1381 caacagcaat catcggacta gcactaggta gcggcgttat cggctacgca gcaagcaatg
     1441 cgtttcaaga tttggacacg atcaaagcaa atttcaacac agtcctacaa tacggtcaaa
     1501 cgaaatcgca acgtgtgtca gaacttgaat cacagctatc caacaacact cgcacacagg
     1561 agcagctgaa agccgaaatt gagcaaatca aatcggacaa gcagaaggaa attgaagcca
     1621 agcaacgaga agtcgaacaa aagcaacagg agatcgctac aaagcaacag gaagccgata
     1681 gcttgcgcca acaactatcc acagtgcaaa acgacaagga gcagttagaa cagcgcgtga
     1741 gcgagttacg gcagtatacg gatcaaaagg taggggagtt ggggaaatga aagagttgac
     1801 tatttatgac ccaaatattt catgggctaa acacacaatt aaagtttctt ttatgatttg
     1861 gggttacaaa ggatatgtta cttacaaagt gggcggtaac actaagggtt tatcattaat
     1921 agctattgat tcagatgatc tatatgatgc taatttcgag gataacccag ttaatttccg
     1981 tgaccttgat gaagattggt tctctatgga acttactaac gataaaggcg acagtacact
     2041 tgtagaagat gaattcgaca gactaggtga ttatattgtc ggtgttgaaa ttattgcgca
     2101 cgaaccagag taggagagtg aatacaaaat gattaaaaga atcactatat cagttgaaga
     2161 agatagtgga tcaacaatgc agcataccga caatcgaaaa acttttttaa ccaatagaaa
     2221 ggagaatttt gatgcagatt aagaaggcga cggatatcga acaaaccaag ggaacatatt
     2281 tgatctacgg tgctccagga aaaggtaaaa cttcaacggc taaatatttt ccagggaaaa
     2341 cactgatcct tgatattgat cggacttcaa aagtccttag aggtgagaaa aatgtcgata
     2401 tcgtatatat cgacaacgaa gatacctgga acgactgggg caacacgttg gctgatttaa
     2461 ctaccaatta tgtaggtgtt tatgacaacg tggttgtcga taatgtcagc gaattggaac
     2521 ggtgtattct gtccagttta ggcgcagaag gaaaaaacaa cggcgttcct tcgcaaggcg
     2581 attatcaata tatgcaattt agaatggtga acagcctacg ctatatgaaa aatttagaca
     2641 gcaaccttat ctggaccgca tgggaagaaa tcgatttgtg gacggattcg gatggatctt
     2701 cttacaacat cgctttgccg cagatcaatc gaaagattcg aaacaatatt ttgggattat
     2761 gtgacgttgt tggtcgattg atggtaaagg aagacgatga acgcggattt attttacaag
     2821 ccacagattc aacctatgcc aaaaatcaat tggataatag atctggctgt aagcaaagtg
     2881 agctgatttt agatggaatt gtacgactac cagaatgatt tagtcgatcg tgcaagacaa
     2941 gcctatgttg atggttacaa ggcaccatgt atcgtatcac cgtgcggttc tggaaaatca
     3001 gtcatggttg cagagatcgc taaacgaacc acacgaaaag gcaacagagt gttgttcttg
     3061 gtccatcgtc gtgaattgat tgatcagatc aaagatacgt ttcaaaagat gggcgtagat
     3121 aatcagcttg taaatttcgg catggttcaa acagtcgtac gacatcttaa aaccatcaag
     3181 aaaccacaac tgatcatcac tgacgaaaac catcacgggt tagcagcgtc ctacagaaaa
     3241 atctatgagt attattcgga cgttccgcga ttaggtttta ccgcgacacc catccggtta
     3301 aacggtagtg ggttggggga tgtgaacgac ttgctaattg aaggagtatc ggctaaatgg
     3361 ttgattgaga atcatagatt agctccttat gaatattatg cacctaaatt gatcgataca
     3421 gccgaattaa agaaagcttc gactggagat tttacaaaga aatcaatgga taaggcagtg
     3481 aagaatacta tttatggcga cgtgttgaaa cattataaaa cgttagcaga aggtgaacag
     3541 gcaatagcat actgtcacag tatcgatgca agtaaacaca ctgctgaaat attcaacgat
     3601 gctggttata aagcaacaca tatcgatgca aaaactccaa aagatgaacg tgcagatatt
     3661 atcgaagcgt tcagaaatca tgaaattaaa atcctatgca atgttgattt gatcggagaa
     3721 gggtttgatg ttcctgattg ttcgactgta attatgttga gacctactca atcgttatca
     3781 ttgtacattc agcaatcgat gagggggatg cgttaccgac cagataaaac gtcaatcatc
     3841 attgaccatg tgggtaatgt gaaccgtttt ggattgcctg atatggatcg tgaatggagt
     3901 ttagatacaa aaaagaaaac aaaaactgat agtgatttat ctatcgttca atgcgcgttt
     3961 tgttttggtg cttacgaacg gccaaaaggc gataatattt gtccgtattg cggagagttg
     4021 cagccgattg aagaacgaaa gtcagagtta gaaatcgatg aatcggctga attaatgaaa
     4081 gtcggagaaa cgaaaattac tttgaatttt gagaacaata aatattacaa catgacggaa
     4141 gatgaagcag aaagtataga ggacttatat gccatagcaa gagctaaagg atttaaacct
     4201 ggttgggctt atatggctgc taagaggaag gggtggttgt aggaatgggc ggtatttttg
     4261 gtaaaaaagc agaagatgtt caaggttcaa taataaatgg cgtaacaata ataggtgata
     4321 ctggaaaaag agatagttca ggcaatcaaa ttgtaatcgc tagaaataaa aataacgaag
     4381 tttttgaatc ttctctatct aatataaaaa gaggtgttgt taacgggaca ggcgaagaga
     4441 gtggctacgt taatttaaaa aaagctaatg aaaaatacca tgttgaagga acttacgtta
     4501 aggctttgtc tgtcggtgga acaaaaggcg ttaattggga taaacgaaga aataaatgga
     4561 aagcacgatt atatttcaaa ggagaatatg tcttacaaaa ggatttcaac accaaagaag
     4621 aagcaatcca agcacgactt gcagcagaag aaaaatattt caaacctatc ttagaaaaat
     4681 atgaaaataa aaaggagaat gattaacatg acaggattta acttagactt ttcaaaagcg
     4741 caacaaggga acgaaatcaa agacggtacg tacgaagtgg tcgtaaacaa agctgtagaa
     4801 aatgcaacta aatcaggcgc agaatttatc gatatcgatt tgatcgttcg taacgatgtg
     4861 gatcaaccgt tccaaaataa acacattttc gcaaaaatct ggaaagccaa agctaccggc
     4921 aaatataacg aaggtatgat catggcaatt gcacaagcgc tacagttgga agacggaaaa
     4981 tcatataacg ggtttgatga attgctggcg gactttgttt tgaaaaccgc ctcagtacgc
     5041 gtgaaaactg aagagtcaaa tggatataaa aacgtcaatg tgaagtcttg ggataaaacg
     5101 aacacacggg gcgtaatgaa tcaccaattc aaaaatggtg atgaaccatc cttcggaccg
     5161 gaacgttcaa gagcgacaac ggttagaaat gacaatcttc cattttaatt gaggtgtaat
     5221 caatgtatga aaaaattcca aaagagctta aaaatctcaa acaatggtgt gtttataagc
     5281 tagtttggga tgaaaagcgg cagaagcaca cgaagatccc ttataacgcc aacaacgggc
     5341 ataaagccaa atccaatgac gagagtacct ggtctgattt tcagaccgct ctcgctgcta
     5401 ttgatcacta cggaatgagt ggactggggt tctttttcaa accaccttat ttcggcattg
     5461 atatcgataa tgctgaaggt gaagttgagc gatttaaatc aggtgatata gaagaaaata
     5521 ttatctatga atttatcgaa agcatgagat cctatgctga gtattctcaa tcaggtactg
     5581 gcatccacat tattgctcgc ggagaattgc ccggcggaag acgcaggaaa ggtgacgtag
     5641 aaatgtacca gaacggccga ttcttcgtta tgactggaaa tgccgcatca aaatatttag
     5701 aaataactga gccgaatcca aaggatatca aacgcctata tgatcgctac gttggagatg
     5761 aaaaaatcat ccaattcaaa gaagaaaatc cgctgatgaa taccgttgat ttaccggttg
     5821 aagaaattat caaacgtgct gaatcgtctt cccaaggggc aagattcaaa gtctttatga
     5881 acggcggttg ggaagctttt tattcctcgc aatccgaagc ggatatggcg ttcgccaacg
     5941 acttggcttt ttggaccggt agagactttg aaaaaatgga tgagattttc agagcgtctg
     6001 caatgatccg tagcaaatat gaccaaaaaa ggggatcggc aacttacgga gaatctctac
     6061 taaataaggc aattgctgat acaaattccg tctacaaccc caaacggaaa tctgatttta
     6121 agatttttat caaagaccag gaacagccaa aagaggaaaa atactattcg tacgacgaca
     6181 ccggcaacgc ggatcggttt actgatatct acggaacgtt agtcaaatat tcgtacattg
     6241 ataaatcttg gtattactac gatgggaaag tttggctaca ggacaatacc ggcgaagttc
     6301 ggaaaatgat cgatacgaca gtagacatca tgggaaaaga accgttaacg atccctgaag
     6361 gcgcagacga tgaaaccaaa gaagctttga tgaaagcgaa agaaaaacac gtcaagcgtt
     6421 ctcgcagcaa tgccggaaaa aacgctatga tggacgaatt gaaacatcga ctatccgtat
     6481 tgccggaaga gttcgacaaa gataaaacgc tgttcaacac acaaagcggc tatctgtctt
     6541 tgcatgacgg tcttctacac gagcatgaga tagacaaaat gtttacacgt gtttcaaatg
     6601 tcgagtacac agaatcggtc gattgtccta tgtgggaaga atttattaat cagatttttg
     6661 acaatgatca agaattaatc agatatatcc agaaatgtgt cggatattcc ctgaccggat
     6721 ctaccagaga gcaatgtatg ttcatcctct acggtcatgg gtcaaatgga aagtcggtat
     6781 ttttggaaat tatttctgaa ttgatgggaa actatgcgat gactatgcag gcacaaacga
     6841 tcatggtcaa gcaaagccaa tcatcagcaa acagtgatat tgcacgattg aaaggtgcgc
     6901 ggttggtcac ttcgtcagaa ccgaatgaag gcgtccgact ggacgaaggt ctggtcaaac
     6961 aattgactgg tggtgataaa gtcacggctc ggcaccttta tggaaaagaa ttcgagttcg
     7021 aacctgaatt caagttgtgg ctggcaacga accacaagcc catcatccgt gggaccgatg
     7081 acggcatctg gcgacggtta aatttaattc cattcactgt ccaaatcccg gatcacaaaa
     7141 aggataaaaa tctgaaattt aaattgcaga cggagttgca agggatcctt aaatgggcaa
     7201 tcgatggttg cctgttgtgg cagagagagg gcttggagaa accaaagtcg gttgttgcag
     7261 cgagtcagga ttatcgaaac gaaatggatc aaataggtac atttatcgaa acgtgttgcg
     7321 aaacaggtcc aggactaaaa atatcaggcg gagaattata caaagtttat cgtgaatggg
     7381 catcagataa tggcgagcat actttcacta acacgaagtt tggcagagag atttcaaaga
     7441 aatatagcaa agaaaaagct ggtggattta tggtttataa aggaatcaca ttaaaaccaa
     7501 gaaaatacga taacgttaga gaacttttta aataaatctg gagggttctg gagggttgag
     7561 aacgaaaccc tccaaccctt agaaacgttg atatatcagt atttatat
//
LOCUS       DAFGFZ010000130         7509 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000130 DAFGFZ010000000
VERSION     DAFGFZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.132"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            82..537
                     /locus_tag="LR726_002537"
     CDS             82..537
                     /locus_tag="LR726_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217575.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            656..1714
                     /locus_tag="LR726_002538"
     CDS             656..1714
                     /locus_tag="LR726_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBL6217576.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            1756..3705
                     /locus_tag="LR726_002539"
     CDS             1756..3705
                     /locus_tag="LR726_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL6217577.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            3708..7349
                     /locus_tag="LR726_002540"
     CDS             3708..7349
                     /locus_tag="LR726_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217578.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVVYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMIDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         2687 a         1118 c         1581 g         2123 t
ORIGIN      
        1 ttatttatgt tattatgata ttataataac ataaatgtgt tacgcccgaa cctcatttta
       61 aaaaaatgaa ggggtgagac aatgaatgct attaattggc atgaatttgt taactatatg
      121 gagagcttac tagatgatct aaaaagtttt agtattgctg atgcagtaaa gcatgggtta
      181 ccagttagta caacgatgaa attacgagga ttgaaaattc cagatgaaat tattaagctg
      241 agaactaaaa caattattga aaccgcaaaa gtcgttgaca atatgaacca tgaactaaaa
      301 ggcaagaaaa caacttacga gaaagatttt gaacgttttt taaaatctgt tattggaaaa
      361 tatctaggta aaaaatcttt cagtgaattg agagatgaag agattgtacc tactccaatg
      421 gttagcaaaa tccgaaaaaa caaagccgag gggaattact atcttggtac aatagcaaaa
      481 atcattgatt atttggaaaa taaagagcaa aagaaaggaa cgaagaaagg aagataattt
      541 ttgtgttaaa tattaatggg gaagaatata ccgaaatgat taaaaatccc gaacctagaa
      601 ttaccgaaaa aacgtctgat tgtttgctct tttatgaaag ggggcggaca aactaatgaa
      661 aaaaaacgtc aattcaacat ctaccaaaga tcaatacagt attattggag ggttgcttgg
      721 tgataaggat aagagaacac gtcacggatt acgcattatg cttgcaactt ttctaggagt
      781 gcttttttta ttaacaggct tgggtagcta catttatcaa ttaggaaact ataattatta
      841 tctcgtgaac aatacaacac ctataggctc ttcattggag tttgctcgat caggagcaga
      901 gatcactatt tcagatgtgt ggacagatca aaacagagat gtaacagtag taaaactcgg
      961 ttactcgcaa aacgctagaa agttgttatc tacgcaagga aaaaactaca acttatatat
     1021 ggcaacaaaa gaaaaagatc gtcctaagaa gcttgaaatg agctatggaa tgttaagtac
     1081 tgatggggat gctttcttat tcctaaaagg taaattagat gaaagagctt atcaagtttt
     1141 tattgcaaac caagtatcac taatttctgg tgatgataca gctgatgatg ataccaaagt
     1201 caatgtgaat gaagcagatt cagttacaaa agcactttct caatattcta tgaatgatgt
     1261 taataatgaa ggagtaattt cttttgggaa aaaagcaaaa cctgttaata ctcctgataa
     1321 tatcaatttc agaataaatc catattcaga ttcaactaat gtctatcaag gaagctttct
     1381 taccaaagat ggcgatattg attattcaaa agttgtagca gttacaagta agaataatgt
     1441 tgtaaataag ctagaagatc agttaaatgc ttcaaatact aacatcaaga aaattgaagc
     1501 tacaaaagct gagtatctgg aacgtttaca acaaaatcct gacgataaag attcgcaaga
     1561 aagtattcgt caactagaca gtcgaatgga gaccgaaatt aaagcaatca atgatacaca
     1621 aaaattgatt gatcaataca aaaatgctga ctttaacaaa gattcttttg gagacatgaa
     1681 gacgacattt aagtacctaa ctctgactga ctagaagtaa attattctta ttttagagga
     1741 agtgaacaca aacagatgga aaatagtacc gagtttttta agaatattca aactgaattt
     1801 aatcatgctc tcacaccaat gaaaccaccg aaacataagt tagtgggacg tgatgacgaa
     1861 ctccatgagt tgtcactgat cttgaataga ccggaaacac cgcaagctat cttgttaggt
     1921 gaagccggaa caggtaaaac agcacttgca agtgaatggg ctgaaagaca gcgacaagat
     1981 ggaaagaaag tacaagtata caaattatca atcggacaat tgcgtgttgg gggagatgat
     2041 gtcctccaaa cacgtttaga aacattaatt ccaagattga aagaatatga gcgaatttta
     2101 aaatctgagg acaaagaaaa tgaatttgtc ctcttcattg acgagtttca taaagttgtt
     2161 agctcttttg gtagtggcaa taaaattggg ggtgattcgc taaaagaacc gttagcaaat
     2221 gaatcacttc aattgattac cgctactaca agaaaagaat atgaaaacta tatcaaaaga
     2281 gatgatgcct tagggagacg gttacaaccg ttagaattga atgaactaaa aagtgatgtt
     2341 gtcgtcgata ttttgagaca atggctaaat acatcctctg ttaaagatga tcctcaggct
     2401 tcttttaaat tatcgcaaag gattcctgat gaattacttc aagaaatcgt acaagcaaac
     2461 aagctttatc gtgaggattt gtccgaaccg gctaaatcaa ttgccgtaat ggaggatttg
     2521 attgcaaaag atcgagtaga cggaaatgct tataatcgag aaagtctatt gcaaatattt
     2581 ggtaagtata atattcagct agattctgat tttgatatgg aaaaacttca agaggaatta
     2641 aagtccataa aagggcaacc gttagcagtt tatactataa caatgttgtt taaacgtatg
     2701 aagcttggat ttgatcgttt taaaggtcga aagcaaccaa aagcaactgc tctgtttaca
     2761 ggatcaacag gggtcggtaa aactgcaatg gctaaagccg tggcaagagg actttatggc
     2821 gatgaaaata aagttctctt agttaatatg ccggactaca aaacaaaaga tgatgcaatg
     2881 cgttttagac gactcatagg tactcatgta aggcatgatc cttctgcgat tattgtgtgt
     2941 gatgaatatg agaaagctgg tgtagaaaca ctcgatgcct ttttatcaat tttagatgaa
     3001 ggtaggataa gttttactga aagaggatgg gatgatcgag aagttcacga caccgtttca
     3061 ttgtcaaata caattttatt tgcgacaaca aatgcaggtg cggagatgtt cgattcgtta
     3121 aacagatata gctcactatc tcgtgttaca aacaaggaaa attctaatga atatagcact
     3181 gaactaaaaa gagaataccg aaaagtcgca tcagggatca aacaagcctt gattggtgaa
     3241 ggtctaaagc ctgaagttat taaccgattt ggacgaatag ttccattttg ggctttatcc
     3301 gaacaaactc ttctggaaat tgcttataat gaaatgttga agagtgctga attagtacga
     3361 aaacatttaa atatagattt acgtcttcca cctaggaaag aaagagtata tggtcaaaat
     3421 ttttatgcag acgatctctc catgcactta gtttttgaca tggcttcaca agaggattct
     3481 aaatcagggg gtgcaaggaa tatcttgcat ttagttgaat ctgagtttga agaagcgatt
     3541 actgaagcaa taattactta tccaaacgca aaaatattcg atgttaagtt gagtgaagcc
     3601 ggagcgtttg aaggaaaagg tgattctaat gctgaaatta ttgcaaagcc ttccgttgct
     3661 gaaagtaaag aaaacaagat tgtattttcg attggagggg cgtagctatg aaattcaaga
     3721 aatatttact acaaaacctt agaataaaac gatgggtcgt aagaggtcta gttattggag
     3781 gagtattagc agttacttta ggcgctcctt ttggaatgaa tgatccaact cctgacaatc
     3841 aagatagcaa acaagaaaca gttataaata agaacgttgt atatgcggaa agtaaaccgg
     3901 cagaacaagg aatgtggaca ctatatgctc gtatcctaat gggagataaa gaagttaatg
     3961 atagtgccgc taaaactgga caaatgaatt tgttaggaca aggtggtatc agtgccgatt
     4021 tcccttattc tgacatttct aaacaaggta attcgattgc agggggtaat gcaggtactc
     4081 aactagcatc tactttagca acttatagtt actacggata tattgaaaca gtctcaggaa
     4141 atgctattgc cagtcaagca tcatccattc taggaagtat tggtaggacg attggtagtt
     4201 ttatcggatc aattagttta atcctttcta atattgctga atccataaat gaactccttg
     4261 gaaaattttt catgagtatg aatatatttt ctcttatggg gtggggagaa ggtggatcgg
     4321 atgtatctaa tccggtatct aaagcattaa ctagcttgct taacaatttg ggattaaata
     4381 atgaattatt taaagcgata gcaagcttat ctatgctctt tttcacagca atactaggtt
     4441 atcaaggaat gaaattattg tcgaaagatg gtgttaactc gaagggatta tcaccagtac
     4501 ttgaaaaatg gattgttcgt atttttgtat tgtttatatt cttaccagtg gcaggatcgc
     4561 tattgggagt ttatatcaac ggcatccagt cagcaaaaaa agatggagat ataccaacaa
     4621 atgttgtttc tgactacatc attaacacaa gactttgggc ttctactcaa aatcttgctc
     4681 catcatctga tactggtggt gatgtacctg acggaaagcc tacatccgaa atcaagttcg
     4741 ttgatacaga ttatgaacca tctaaaaaat ctgcaatgat aagagcgatc aatactagca
     4801 gttatgccag acaaaataat gatctagatt ctaattcagt cggtatggct ctattaaaag
     4861 aatggtcatc gaatgatact ttcaatatta atacttatgc cggtgatgtt aatgtcgccg
     4921 aaaatgatgc ttatatggga agagcgcctg ctgtttggac aggctataat ttaggggatg
     4981 gaaaagaagg aagtgcaatt aagaaaccaa ccaatttaaa tggatatatt tggtcagcaa
     5041 atcaaaatgt gacagacgac aatcgcaagc ctgatggaaa aggaacagcg cataactttg
     5101 atgctactca accttggggc gttgctaaca atgcaacttt tagcacacag tcggtagctt
     5161 tattgctcca atcagcactt gatacatcag gagctaagtt ttatgcgtat aatatcgctc
     5221 cttcaggaat tcaaggcagt gctaagaata tgtcaacagt aaaaacagaa tggcgtacag
     5281 catccttagt tggtcatgga gcgatgggta agtttggctc gtggctagcg atgaacgctg
     5341 aatcaatcgt tttagcagtt ctttacttag catgtgcttg ggcgatgttc aatgtgaata
     5401 tcgtgaaagc atttaaacgt gaatttattc ggatttggga agtagttcta acaggatcac
     5461 ttgcatcagg agcaggtgtg atggcaattt cattaggtgt tgttacaaca tctctgatcg
     5521 gactacaatt acctatcttt ctgattactt ttattcgtgc atttggtgat actgctgtgg
     5581 gattcattcc taatacatta gttggcgatg gcgtagcaaa tattgttcga tctgctatga
     5641 ttttaggtgt aggatatctt ttagtcttta aaaaaggcga aaaagtaaat aatttgatta
     5701 atatgcttat tggcttacca cttgaagtag cgtttgggtt tgatgaacgt gctaagttat
     5761 tatttggtaa tagaaatgat gctgtgcgag ttgtggacgg cgtttctaaa gcaatcaaga
     5821 aaagccaagg agctaaaaat ccctattatg atggtggcaa cagctcagta aaagatatgg
     5881 ttgcaggagg tcttggagca gttggagcgc aaaaaatggc tgaacgtttg aacaatatgg
     5941 gagatagcaa tgctgatgct aagtccattg ctgataaagt aattcctgaa acaccagaag
     6001 gaatgtcatt caacggtggc ggagttggtt caacaatcaa taatacctct ttcaaagatt
     6061 tagttcgtca agatcaacta cgtaataact cccaacaata tattgatcat ttaggacgta
     6121 tgattgatga aaacggaaac tttgtcgatg aaaatggtga aatattaaaa gatagtaaat
     6181 acggtaaaga tgctaaaggc aactttattg acgaaaaagg gcgtttggtc gatgaagatg
     6241 gaaaccttat agatactaat ggtaaaccat tggaagatag tgaagatggt aatattggta
     6301 tggatagcga aggacgttta attgatgaaa aaggtcgctt agtcgatgaa gatggaaatc
     6361 ttgttgacga aaatggacgc aggttgctaa atggtgattt tggtcaggat agtaacggac
     6421 gtttagttga cgaaaaaggt cgtttagttg atgaagatgg aaatcttgtt gatgagtttg
     6481 gtaataaact tggtaaacac agcgatggat ataatgtgaa tggagattta gtcgatgagg
     6541 atgggaatat cattgagacg agagatgcgc ttgtggcaga tcgaaataga gaactcgaac
     6601 aacaacggca tacgccaatt gctgttgcaa ttcctgagaa tgaagatgac gagattactt
     6661 tagatcaaac tcatcaagaa ctgaggaatg aaatgagtgg tgaggacgtg tcagacagag
     6721 tgcatcggga attaggagat ggagcatctg gtgaaagtca gtcaagagag cttaacagca
     6781 actttgataa tagtgctatt cggaatgaca cagcacttaa agacagagaa tcgctaacaa
     6841 aagaaacgga taaagaacaa tcagatgtga agaaaaagtt cattcctact cctaatgtta
     6901 atcaaacaga taaatatgaa acaaatattg gatcaggaat gtcgaaagac aatcaatcaa
     6961 ttagagaacc tgaactgaca aataaaaata atagaacaaa taattctaaa tctacttcag
     7021 ataattcagg gaaatctaat caatcaacca agaaaccatc aacagctaaa tcagccgttg
     7081 aatatctatc aaaaactggt aaaaatgttt acacctcagc agataaagct ttagcaaaag
     7141 ctccaaaagg tagtctagca gggataacct atgaagctaa aaacgaagct attaaaatag
     7201 gtaaaggaac ggctaaggtt gccaaggcta ctgctacagg agcatccaaa actggtaaag
     7261 tagtgaagaa atctaccatg tcagtcaagg actatattgt aaaatacggt aatcaaaaat
     7321 caactgaaaa taagtctgaa gacgagtaaa aagaaggagc attgatttgc tccttctttt
     7381 tactttgatt tctaactgaa gatgtcaagg aaaatttcta tttatgcaaa aaaattattt
     7441 taaattgtct ataaatgaat taaatgttta ctgttcaaag ttgtataatt aaaaagggct
     7501 tatgctggt
//
LOCUS       DAFGFZ010000131         7387 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000131 DAFGFZ010000000
VERSION     DAFGFZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.133"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            213..1070
                     /locus_tag="LR726_002541"
     CDS             213..1070
                     /locus_tag="LR726_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL6217579.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1063..1965
                     /locus_tag="LR726_002542"
     CDS             1063..1965
                     /locus_tag="LR726_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL6217580.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1998..2348
                     /locus_tag="LR726_002543"
     CDS             1998..2348
                     /locus_tag="LR726_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL6217581.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2397..3320
                     /locus_tag="LR726_002544"
     CDS             2397..3320
                     /locus_tag="LR726_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL6217582.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3398..4291
                     /locus_tag="LR726_002545"
     CDS             3398..4291
                     /locus_tag="LR726_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL6217583.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4329..4892
                     /gene="amaP"
                     /locus_tag="LR726_002546"
     CDS             4329..4892
                     /gene="amaP"
                     /locus_tag="LR726_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL6217584.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4906..5094
                     /locus_tag="LR726_002547"
     CDS             4906..5094
                     /locus_tag="LR726_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL6217585.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5107..6009
                     /locus_tag="LR726_002548"
     CDS             5107..6009
                     /locus_tag="LR726_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL6217586.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6041..6283
                     /locus_tag="LR726_002549"
     CDS             6041..6283
                     /locus_tag="LR726_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL6217587.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6539..6910)
                     /locus_tag="LR726_002550"
     CDS             complement(6539..6910)
                     /locus_tag="LR726_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217588.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            7134..>7387
                     /locus_tag="LR726_002551"
     CDS             7134..>7387
                     /locus_tag="LR726_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6217589.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT         2669 a         1253 c         1467 g         1998 t
ORIGIN      
        1 gaaaaaggtg gctatatata gtagtttttg gtcaatacct ttgaataaaa agccatgaaa
       61 aacgaatagt tagcgcttac cgatgcgttt tgtgataaaa aaattttaaa atattcaatg
      121 aaaacaaagc atatattttg ctttaaaacc aaatgaggaa tattctagat gtagaacagg
      181 ttagaaaaag attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg
      241 tggtaactgc ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat
      301 tagaaaattg caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag
      361 aatatagtaa agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc
      421 aattcctgac agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa
      481 ctaccgctta cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag
      541 tcctttactt agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc
      601 cgttaaatgg aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa
      661 gattaggaat cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg
      721 atcaatggaa taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg
      781 atcgaatcat ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac
      841 aacttcatcc aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa
      901 atgaaaaata tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta
      961 caggacatga taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa
     1021 aaaaattagc acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata
     1081 gcagtagtcg caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca
     1141 gtattagata attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc
     1201 gattttgaaa gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat
     1261 gtggaattct tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt
     1321 ccagaaacag cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa
     1381 cgtatcttat atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt
     1441 cttgacttcg gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg
     1501 gaaaaaatga aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc
     1561 aatgtgaaaa aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat
     1621 aatccagaaa aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt
     1681 tggttaccac ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa
     1741 catccaacag cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc
     1801 catttttcag gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat
     1861 tacgaaaaat atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca
     1921 aaatatgcaa aagttcaacc aaaaacagat gtagttgtta aataatagaa gacaacaact
     1981 gaagaggggg agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg
     2041 aaaaggttgg ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc
     2101 taagggacaa aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg
     2161 aatttggaga atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat
     2221 tagagcaaaa ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct
     2281 tttcattaga aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct
     2341 tcagctaata gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg
     2401 atgaagaaaa gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata
     2461 gtgcaaagcc cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt
     2521 tcgtaggaac cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac
     2581 tagagcatcc attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa
     2641 aaaaattaac gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata
     2701 gaaaaaccga atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg
     2761 aaaagatcat cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag
     2821 agatgaaaaa gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag
     2881 aactgtataa acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag
     2941 atcaacggga aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg
     3001 atgaacagct actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga
     3061 tctatcgaga tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc
     3121 taaatcatat catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt
     3181 tcgcagggat ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct
     3241 ttatactcgt agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc
     3301 ggaaagatta tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt
     3361 ttggcaatac aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga
     3421 cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt
     3481 acagcagaat atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca
     3541 actagaaaac cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc
     3601 gattgcattt gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa
     3661 agatgcagag gaagtagcaa aatacgtgaa agttgcagga cgtaagattg tcttactacc
     3721 ttttgatctt cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact
     3781 tggcgggcta gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc
     3841 tgagttacca cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc
     3901 tttagtgaaa gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc
     3961 tgttcaagca ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat
     4021 cgctaacttt acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg
     4081 tgtagcacct ggtccaatct ggacaccatt acaactagac gaaggacaac cttctgattc
     4141 gatccctgaa tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc
     4201 accagtttat gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg
     4261 agtaactggt ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag
     4321 gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat
     4381 ctgtgctttt gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga
     4441 agtttcgatt cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg
     4501 ttgcatttgc tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa
     4561 tcggaaactt tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg
     4621 aaggaatggt tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg
     4681 tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag
     4741 cactcattgg taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat
     4801 tgggaacaaa agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta
     4861 aacgaagcaa tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt
     4921 tcgacataca agctccctat catctttggg gtacttggat tgatattagc cgttttatgg
     4981 atcaccgtgg gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct
     5041 atcggttttt atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa
     5101 acatccatga ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg
     5161 tctgcacatc cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac
     5221 aaaacagcac cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta
     5281 gaagagaaga aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct
     5341 aaagttccag aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat
     5401 ccttctgacc atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga
     5461 aaacttggtt ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca
     5521 atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt
     5581 gaacaagtag acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa
     5641 cttgtgaata cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta
     5701 gcagttgata tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa
     5761 atgcaagaag taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc
     5821 aacgctaacg tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg
     5881 caagacaaag taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat
     5941 aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg
     6001 gttcagtaat aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact
     6061 tatcgtcgga ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat
     6121 ggggattatt gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt
     6181 aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat
     6241 cattttagta ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga
     6301 ggtcgtgaaa aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc
     6361 atatttttca tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac
     6421 ggaataagag gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc
     6481 aattagattt ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt
     6541 aataaaacaa tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata
     6601 cgaccacttt gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa
     6661 agcaatggat cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt
     6721 ttactcttgt ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa
     6781 gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga
     6841 gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa
     6901 taaatggcat actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat
     6961 ttgtttgtga agctttttcg tgtcgtaaca aatgttatct aatgtatttt taaataaata
     7021 atcattatta tcaaaataat gtcagactgt agacaaagtc cattttatgg atgatgtcta
     7081 cagtcttttc ttttataata gatttatgaa aacgaatgca aggagtgata gctatgatgt
     7141 ctaaaaacaa tacgaatggt agaaatcaat ttgcgatgct tacgattgat gatctagttc
     7201 ctcaagatca tctcgttcga aagattgatg ccgcgcttga ttttgaattt atttatccaa
     7261 tcgtcgaagc cacttactcc gatttaggtc gcccaagtat cgatccagtg attctgatca
     7321 aattggtgtt tattcagtat ttattcggta tccgttccat gcgacaaacc attaaggaag
     7381 tcgatac
//
LOCUS       DAFGFZ010000132         7287 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000132 DAFGFZ010000000
VERSION     DAFGFZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.134"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(57..350)
                     /locus_tag="LR726_002552"
     CDS             complement(57..350)
                     /locus_tag="LR726_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL6217590.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="LR726_002553"
     CDS             complement(378..1043)
                     /locus_tag="LR726_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL6217591.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="LR726_002554"
     CDS             complement(1183..1815)
                     /locus_tag="LR726_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6217592.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="LR726_002555"
     CDS             complement(1822..2889)
                     /locus_tag="LR726_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL6217593.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="LR726_002556"
     CDS             complement(2886..3617)
                     /locus_tag="LR726_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL6217594.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LR726_002557"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LR726_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL6217595.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LR726_002558"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LR726_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL6217596.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="LR726_002559"
     CDS             5780..6949
                     /locus_tag="LR726_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL6217597.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         2203 a         1479 c         1119 g         2486 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaat
//
LOCUS       DAFGFZ010000133         6990 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000133 DAFGFZ010000000
VERSION     DAFGFZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.135"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..224)
                     /locus_tag="LR726_002560"
     CDS             complement(<1..224)
                     /locus_tag="LR726_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217598.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHG"
     gene            complement(432..1358)
                     /gene="glsA"
                     /locus_tag="LR726_002561"
     CDS             complement(432..1358)
                     /gene="glsA"
                     /locus_tag="LR726_002561"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL6217599.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1381..2592)
                     /locus_tag="LR726_002562"
                     /pseudo
     CDS             complement(1381..2592)
                     /locus_tag="LR726_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2627..4240)
                     /locus_tag="LR726_002563"
     CDS             complement(2627..4240)
                     /locus_tag="LR726_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217600.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(4352..4561)
                     /locus_tag="LR726_002564"
     CDS             complement(4352..4561)
                     /locus_tag="LR726_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL6217601.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(5550..6764)
                     /locus_tag="LR726_002565"
     CDS             complement(5550..6764)
                     /locus_tag="LR726_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL6217602.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6892..>6990)
                     /locus_tag="LR726_002566"
     CDS             complement(6892..>6990)
                     /locus_tag="LR726_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL6217603.1"
                     /translation="IKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
BASE COUNT         1962 a         1507 c         1150 g         2371 t
ORIGIN      
        1 ataccatgtt tgaatacgag tttatactta tgcttctctc ttacttgttc ttttatacaa
       61 tattctagac actcggctag actttctaga tcgtgaagga tcgttggtgt agtcaccctt
      121 agagaaatag ctgtttgttc gatgtctatt tcttggaatt caaataattg ttcacacaga
      181 tacacttttc taaatatatt tcgctcgatg taattttcca gcattcctga tacacgcctc
      241 cagtttttct tatgatttta tttaatcata tctgtaaatc gtatttttgt ttccttttta
      301 tttagaaaag ccctttcaaa aaccaataaa aaaagaaact gggacaaaag tcaaacaagc
      361 tcacaatttc agcctcattg taggtagctt tgagccgttt ggacttttac cccagcctct
      421 ttcaagaatg gttattcaaa gatgtcagcg tctagttctt tgactacatc tttcaacaat
      481 tggataccag ctgcgctgtt accaaatgga tctagtgccg ggctgaagac acctatgccg
      541 taacgatttg gcacagctgc catcaacccg ccgcctacac cgctttttgc aggtacacca
      601 acatggacgg aaaactctcc tgattcatca tacaaaccag cagttgtcat aattgatttg
      661 acgattgtag cactttcttc agtaatgatt tgtttgccat tccaaggagc aattccttta
      721 tttgccagca cagcagctaa ttttgctatg cctgtagcgg tgacattgag ggagcattgt
      781 ctgaaataag catcgagtat ctccggtacg tctccttcga tcatttgatt acctttcata
      841 tagtaagcaa gagagcgatt gatatcacct gttcttgatt cagaatgata gatttcttca
      901 ttcaatgtga tattaggatc atcacagatc tctttcataa atgacaagat tcgctcaaat
      961 ttttcatctg catctttacc ggcaatcaac gaggttgttg caatcgctcc tgcattaaca
     1021 aaaggattca ttggatgttt gcgatgattg atttccatat tcatgataga attgaaggca
     1081 aaaccggttg gttccgtgtt gattgtttga aagacttttt ctattccgtt gtctttgatt
     1141 gctaataaca atacgggaac ttttgacatg ctttcgatgg caaagcgcac atcggcgtct
     1201 cctccctcaa tcagttgttt tgtatccaaa tcataaatcg ccatacctaa ttgtttggga
     1261 tccacttcag ctaatgctgg aatgtaatcc gccacttgtc ctgtttgata atagtggaaa
     1321 ttttttccaa tcaattgttc taattctttt acttccattt gaataaccag ctttcatttt
     1381 ttatttttct gcatgctgga atttatgacg gcatgcgtag atcgtaaatg gaataactaa
     1441 gacgataaga taagaaacaa tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat
     1501 actagcaggt ggaacaaatg agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca
     1561 agcaacgatt acttttacaa cttttccgcc tggaacctga taggctcttt caagactgtc
     1621 atctttaaaa accagcttca aatagcccgc gaaaaacata aagtacaaca tcaggtagat
     1681 tacaacagtc aatgacatcg ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt
     1741 aaagacggct gcccagattg ttgcaacgat cccatttgcg atcatcaaat gagcaggaac
     1801 accgtgtgtg ttttcttttg caaaatattc aggaagtaat ccttcttctg ctgctacgtg
     1861 aagtgctttt gaaggagagg ttacccaaga agcaatttct ccgatgacac ctaaacaaac
     1921 aagaatcgca agaattttcg caatccatgc aagatttgga tcaatgtgtc ttaataggaa
     1981 ttctaacgat tgaatcaccc ctgtattaag agacaaacca tgttgaggta ctgtagtagc
     2041 gacggtcaat ccgccaaaag tatctaaaat gattgccaga ataaccaata caaacattgc
     2101 taatggataa tcctttttag ggttttccat ttcatttgca tgactggcag atgtttctac
     2161 gcccatatat gctaaaataa aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc
     2221 aggaatcaat gttttcatag acagtatcgt ttgtagtgga gcgcctgaag cgacataatg
     2281 gatccctaag ccaaacaaga ctaatgctgg caacaagata ccagctacaa aaccgatccg
     2341 agcaatcaat gaagtgtatt tggttccttt taactgtgag agtaaaatag cccagaaaat
     2401 aatcaatagg attgccaatt tcagatattt attgttttgt atagcaccaa tacccgttgc
     2461 ataggataat gcaccagtta agaaatacag catggtcaag tagccaactg ttatctcaaa
     2521 ccattggaaa aagattgcgg caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt
     2581 atagacaccg ccggtttccc ttagttaaaa atattactct tgttgattac tcaactcttc
     2641 caataaatcg gtaatatatc tcaatttcct gtattctttc gttatcctca ttcgttgttg
     2701 cttcatgtat gaggattttt tctatcatgg catttaataa tggtgctgtc agttcctttg
     2761 gtacggaata ttccttgatt aactcaatcc acttttcagc acctatcata tcctgttcca
     2821 ttttacttag ttcttctctt aggcttgtta tctgctgttc cagttctatc tgttcttttt
     2881 ggtacttgct ggacaacatc acaaaatttc gctctgttat cttctcacag gctctatctt
     2941 catacatttt cgcaaataaa cggtcaatct cattttgtct gttctcggct ttcttcaatg
     3001 tacttgcctt tttcttcttt tcccgtattc tctctgcatt gccagctttc tgtatcttgt
     3061 tcaatacctt ttcttcgtcc tgctgtactg ccttagccca atactgcaag cgttccaata
     3121 cggcttgata cagcacatca taacgaatgt agtgcataga acaattacct ttgccaaact
     3181 gcccgtagta actgcaagca taataactgt aaggcgtttt atttgtctta ttcgttccaa
     3241 atctcataga ccaaccacaa tccgcacact tgacaagccc tgcaaaaatc ggcgttgcct
     3301 tttcctttgt ctgtctgcgt cttgatttta tctgctcctg cacacgatag aacacttcct
     3361 tgtcaataat cggctcgtga gtgttttcta ctcgaaacca ttcactttcg ggcttacgca
     3421 ctttcttttt gctcttgaac gataccgtag actgcatatt gtgaacactg tttccgatat
     3481 aaacctcact tttcaatatc gccttgacat gagcaatcgt ccactcataa cgcttacttt
     3541 cgggttttcc ctcaaagata tgtgcaaaag taccgtatct tgtaaaattt agccatgacg
     3601 ctgtcggaac tttttctgct cttaactgct ttgtgatttt tgcacttccg taaccttgat
     3661 aggctaggga aaagattttt tcaataatcc attttgtttc atcatcaacc aacagttttc
     3721 ctttgatgtc agggtgtttc ttatatccta acggagcata agcaccgtaa tgagccccct
     3781 ccgcaaactt tgtcttaaat gctgatttga ctttacggct tgtatctctt gccacccatt
     3841 cattgatgat gttcttaaat ggtgcaatct cactttcgcc tttttcgctg tctacaccgt
     3901 catcaatcgc tatgtagcgg acattatgac tagggaaata caattctgta tattgtcctg
     3961 tcataatata atttctgcca agacgggaaa ggtctttcgt tacaacacag ttgatttttc
     4021 ctgcctctat atcctcaatc attctttgaa aactcggtct gtcaaaattt gttcccgacc
     4081 agccgtcatc aatatattca tcaatgacat tcaaatgatg ttcttttgca tataaacgca
     4141 acatacttct ctgtgtggta atgctggaac tttcgccctg taattcatca tctcggctta
     4201 acctaagata aagtgcagta ttgtaaatct gttgtttcat tgtcagttat cctcctttat
     4261 aactaacccg tgtttacaat acctgcttgc aagtaaagta taacacgggt actttttgtc
     4321 attcaatcta tttcttacaa ctgcaccgat tttatgcgat tgtctgcttt gctttttcca
     4381 gcatgacatc agttagaagt tcttccattg ttttgccctt ttcggaaaaa tgctctttta
     4441 ctacaatctt tgtctttcct ctgttgccaa tgccacactt gccatttttc ttttgaattg
     4501 tttttacagc attatcaata acacataccc cctttctgta aaatctttct aagcaattca
     4561 acggcttttg tcatagccca caggagttcc acctctgcat agttcttatg tagccgtacc
     4621 cattgcgtgc gacgctctta atgctcaagc tgtggctgta cgggagtatc attatctgac
     4681 agtacaggtc atggcgataa aagaggacaa atcttttaca agaacacaaa taggttttcg
     4741 ctcgctcttg caggggaaag gtcgtggcgt atttgtttca acggctagct gtctgtcaaa
     4801 cgtattgaat agctttattc agttgtcaaa gaacgattga aagaaaaaaa tcgtctttcc
     4861 tatatatcgc gttggaaaag aggagaaacg taaccaaaat ccaaaacttt ttccattatt
     4921 agtacaaatg gaacggttca ttttggcaat caacggttga ttttctcctt tttcactact
     4981 atctgtacag caaacgtcca tttgctaagt ttaaatggaa aaattttaac ttcttttcat
     5041 gcaccatata gggatagaaa atgtcatttt gctaagttga tgatgaaaaa agaatatttt
     5101 ccttttcaca ccctaactac ccaaaccata ccgttactgc caacaataga atgaaatatt
     5161 ttctttgcac catataggga acggaaacct taaattgtta agttgagaac aaagtatttt
     5221 catcttttca catcaacact caactgctaa tgcctgtttg aatacaaaat gatgatgttt
     5281 ttcctctttt cacagaagac tgtccactaa tcacgaattt ccgtcatttt ttcaaaactt
     5341 ttttcttctt tcacttcata tagggacagc aagccccgat ttaatgacct attttcaaaa
     5401 aaagtttaga tttccttttc tcgcaccata tctgtacgca agtagccatt ttgttgcaga
     5461 tttaataaaa aatttatttt tcttctttca cttcacatag ggaatgaaaa cagctctttg
     5521 ctgactaaat ctgaaatttt tcaattacat taaaaaatac cgtcattctc ttgtggaaca
     5581 gcggtatcta tacgttcttc tatggttgat ttctctaatt gtaataaatg tttatgcttg
     5641 tcttttagtt ctgcctgctc aatcatatct ttcagtatcg ttgtacgatt ttctctgtct
     5701 aatgcctgta ccatttttat ggttggtgcg acttgtcttt gtagccaatg caacgctttt
     5761 tgtaaagtgt aaggctctgg ctttgtggtt aagcgtatcg gctctctgtt ttcccctaca
     5821 aaccatgccc aatcatcatt tgttatccat tgactttttg gcttgtcgtc ctctctgtca
     5881 acaaagcgga cataatgatt gatgatagaa aaagcggtgc gttcagcgtc ccgataggtc
     5941 agtaaatcta caaccgcata ataggctcgt tcgttcttca tgcgtatttc aaaacggttc
     6001 ttaatctccg tatcttcaat ttctgtgcca ttcttgacat actgctcata atttttctga
     6061 taagcacaca tatataattc gctacttgtt gaaccgagat ataaggtttc tcctgtattc
     6121 tttggtaaat cgctaccgca tttttctgta ccgctgtaat ctttctgcat acggaagtag
     6181 gacatacatt cgccagcctt gtatttttcc tttaactttg gaatatccaa tattcctgct
     6241 ttatcattga tagccaaatc aagccgtttc atcacaccac cagccgtcat acaatccagc
     6301 atgaagtcat accatgagcg ttcctgtgcc agcagataac attccatttg ccgacagcct
     6361 ttgcctttca gttccaataa aacacctaaa tgctcctgca tggaacacat gatattgatg
     6421 tcgccaagta catatttgct ttcatatccg tattttccat aatcttcata aagcatgtaa
     6481 ttagatttca gttgtaatac atcacggata atcgccaatg cgtccgtagt aggaaagcgg
     6541 acacgaaagt aatcaatcaa taagaatagt ggttcttgcg gattgaaacg ctcaatctgt
     6601 ttttctatga tttctcttaa ttcgtcattc aatggctgtt ttcctttttc aatgcggttg
     6661 taatattcac ggctgatacc acaggcaaca gcaagccttg tttgtgttac tccgtattcc
     6721 tcacgctttt gtcttagttc ttcaataaag tttttatcat tcattcttca attcctccaa
     6781 tcaaaaaagg caactaccat aattgataat tgcctgtcat taaaaatctg tatagaaaaa
     6841 gcagtcaatc ctaagactaa ctgctttgtg tgtatggata taaaattgct tctaatcttg
     6901 aataggtatc caaatttcaa taactgaatt atcactattc caatgaaaac gataatcgta
     6961 tttttcaaag tgtaggataa tatcttttat
//
LOCUS       DAFGFZ010000134         6965 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000134 DAFGFZ010000000
VERSION     DAFGFZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.136"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            1..274
                     /locus_tag="LR726_002567"
                     /pseudo
     CDS             1..274
                     /locus_tag="LR726_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            290..439
                     /locus_tag="LR726_002568"
     CDS             290..439
                     /locus_tag="LR726_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217604.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            520..1116
                     /locus_tag="LR726_002569"
     CDS             520..1116
                     /locus_tag="LR726_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL6217605.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1129..2028
                     /locus_tag="LR726_002570"
     CDS             1129..2028
                     /locus_tag="LR726_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL6217606.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2031..2162
                     /gene="ssb"
                     /locus_tag="LR726_002571"
     CDS             2031..2162
                     /gene="ssb"
                     /locus_tag="LR726_002571"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL6217607.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2184..2516
                     /gene="ssb"
                     /locus_tag="LR726_002572"
     CDS             2184..2516
                     /gene="ssb"
                     /locus_tag="LR726_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL6217608.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2538..2795
                     /locus_tag="LR726_002573"
     CDS             2538..2795
                     /locus_tag="LR726_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217609.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2954..3076)
                     /locus_tag="LR726_002574"
     CDS             complement(2954..3076)
                     /locus_tag="LR726_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL6217610.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3266..3490
                     /locus_tag="LR726_002575"
     CDS             3266..3490
                     /locus_tag="LR726_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217611.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            3938..4144
                     /locus_tag="LR726_002576"
     CDS             3938..4144
                     /locus_tag="LR726_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL6217612.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4144..4395
                     /locus_tag="LR726_002577"
     CDS             4144..4395
                     /locus_tag="LR726_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217613.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4411..4848
                     /locus_tag="LR726_002578"
     CDS             4411..4848
                     /locus_tag="LR726_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217614.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            4841..5536
                     /locus_tag="LR726_002579"
     CDS             4841..5536
                     /locus_tag="LR726_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217615.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            5917..6096
                     /locus_tag="LR726_002580"
                     /pseudo
     CDS             5917..6096
                     /locus_tag="LR726_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6158..6342
                     /locus_tag="LR726_002581"
                     /pseudo
     CDS             6158..6342
                     /locus_tag="LR726_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6376..6807
                     /locus_tag="LR726_002582"
     CDS             6376..6807
                     /locus_tag="LR726_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL6217616.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         2495 a         1161 c         1424 g         1885 t
ORIGIN      
        1 actgattttg gatcgacgtt acaagaagtg aaagcagcca gtgaaaagca agaagccgcg
       61 cgtcatttag gaatttgtcc aaaatgccag gaacaagaag tcttactcta tcaaaaggtg
      121 gcagcttgta cgtctgaagc ctgtgatttt aaaaggtcag aagggttcat ttgaagcaac
      181 gattgtttta aaagaagact tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa
      241 cttcaagaaa agaacacgcc gcactgcaaa ataatggagg gttaatctta tgcgtaatac
      301 cacacacaaa caacaagaaa tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa
      361 aatgcgtgcc agcaaacgaa gactgcagca attgaagaac cagcagagga ttcaaaaaga
      421 aatcgaatta gatcgataaa atcttcctaa ctattgtata cgtataaact tatgttatac
      481 tgtttttata agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtatac
      541 aagctgctag aagaatttaa tggaactttg gctttgaagg atgccaaaaa agcgggcatt
      601 tctcctgtaa cgatcaaacg tatggtagat cgaggggagc tggatcgaga gtatccaggt
      661 tttttcacct tacctggtca atttcctgat gaattgttta tggcgcaaaa aaaatatgaa
      721 cgcggcatta tttcacatat taccgcgtta gatttgtatg atttaacaga tatgatccct
      781 agacagatcg atctgactgt accttacggt tatcacgttt ctgaaaaagg attgaaggaa
      841 ttcgcagttg agctacacta tacaaaagcg gaatggtatg aattaggtaa gattgaaata
      901 aaaagtcgct atgggaatcc tattatagct tatgatccag aaagaacttt atgtgacatt
      961 tggaatccct ggtataccgt tgaggatgaa atcaaagtaa aagcaatcaa aaactatatg
     1021 gagtcggaca gaaaaaatct tagaaaattg aatgagtatc gacgaatttt accaacagac
     1081 aaaacgatgc gttcttatat tatggcttta aattaaagga gaagatacat gacacctacg
     1141 cagttaaaag caaaagtgaa gaatatttct agagaaaaag aagttgatcc gcagcttgtt
     1201 ttaagacatt ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa
     1261 aattttgtgt taaaaggcgg cttcttgatt ggctcaaagt atggcattga aaatcgcaca
     1321 accaaagata ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attaacaacg
     1381 gttctaaacg aaattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga
     1441 atgaaagaaa cgagagaagc cgattactat ccaggattta gcttacgtgt gctggcacat
     1501 ttggaaaaca tgcggccaga ttttaaggta gatgtgacta caggtgactc gatctatcct
     1561 gcaacgatca ctcacagtca taaattgatg tttgaggatc gaacgattcc tttggaatcc
     1621 tatcctacag aacaaattat tgctgaaaaa ttaagtgcaa cctttgattt tttaacagat
     1681 aattcgagag gaaaagattt ttatgatctc tatactattc ctaaaatgga gaagatcgat
     1741 gagaagacct tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg
     1801 ttaaagatat actatgaaaa ggatatggat gtactgaaaa atagtcacga gctaaaagat
     1861 acctggaaga aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgatgagaca
     1921 atggattcga ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa
     1981 ctagcttatc aaagacacca acagatgaat agagaaatgg agagataaag atgattaata
     2041 atgtgacatt agtaggacgc ttaaccaaag atcctgattt acgttatacc gcaagtggta
     2101 ctgcagtagc gacttttacc ctagccgtca accgaaattt cacgaatcaa aacggcaatt
     2161 gagaagccga ttttattaac tgcgtgattt ggcgcaaacc agcagaaaca atggcgacat
     2221 tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg acttacgaca
     2281 accagcaagg acaacgtgta tatgtgactg aagttgtagc agataacttt caactattag
     2341 aaagtaaagc agcgacagag agccgtgcgc acgctgatca atcgtccacc agtccaagca
     2401 ccgccacctt tgaacaacgg aatacagcga cgcctaacaa caatggattg aacgcttcac
     2461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttagtttg
     2521 agaaagaagg agtaagcatg gcaaaaaaaa ttagtctgac gatggatcta ttgatacccg
     2581 taattgcatt tatagacggg atttctttgg gcgtcactga aaatcctttg gtaaatagaa
     2641 ttgcgaaagg atcactattt cttgtattcg tgtatcttgt ctgttcttat cttttaaaaa
     2701 gagcaggaaa ggataattcc ttttggcgat tgactaaagt cacagtagca gcgacagctg
     2761 ttatacttac ggctattcta ccgctttata tgtagagtta acgggaaatc gttgaacttc
     2821 ttgcgaaata ttgctataat gaacatgaaa aagaggagtt gcgctaacaa cccctcatta
     2881 acagcaccga ttaaagaacg gtggcagaat attagttatt tggaaataac cgctgacctg
     2941 ctaaagtaga cggttatttc tttttgtcat ttttaagcat atttacaatc aaactaatca
     3001 aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat gcggacaaaa
     3061 ggcttactcc tttcatagat tttagcactt acattcataa gcaccaccac ctttccagaa
     3121 gtcgccaccg tcttcaaact ttactgtctt gactattata gcatatctaa aatcgaataa
     3181 aaattctcaa ttttagctgt ttttgctata attttagcga ggtgatcgtg atggaacaat
     3241 atattggttt actcgataaa ttagggtgat caagacacag ccattgcttg tgcgatttac
     3301 attacgcacg agtcatgaac caatcaattg tgtagtggcc aatatagaaa tagcggataa
     3361 attgctgatt ttggaagaag gcaagtatac tatttcagtg acaggtcact tcaataaacg
     3421 aaatcaacta gtcattgaat caatgcaaat tagaaaccct gatcatttta cgagattaat
     3481 gggcatataa ataaaagaag agctgcagtc ctaaatgagg attgcagctc tttttgtttt
     3541 acatagaaac aagaaaaatg aaaaggcctt tttaggaagg cgcaagcgcc ttctcaattg
     3601 aaaaacctct ctctcactcc aaccgccacc gccgttcgag aagaaaggaa gtgaacgttg
     3661 cacgttcact aagataaggg gtcttgcaaa gcaagacaaa atagaaaaaa cttcctctca
     3721 ccaaaaggtt cgttcctgtt cgctatctct ttattgattt gccagaccgg caacaagttg
     3781 ccttttatca ccaaaaagcg acaaggacaa gggtaaatgg cacgctgcac gcccttgtcc
     3841 ttgtcgcttt tagttgattc attctggcca acaaatcaat aaagaaagaa ctaacgaaag
     3901 gaagtaaaac ctttcctaag aggaggcaga atcaatcatg cgaaagaaat ttgattattg
     3961 gggtgttccc ttcagcgaat tattcccaaa ctatcatgca ccatatactg tcgaatgcga
     4021 ttgtggagaa agagcgaaat gtatcaaaag ctattgtttg tatcaatgtc caacatgcgg
     4081 gaaaaaatat accttgtctt atggcgatta tattttgatt gatgagaaag gaaagaaacg
     4141 ctgatgaaaa cacaacacaa attattgact gtaaaagaag tcgttcaaat tttggtggac
     4201 aaagaagtca tttctgaaaa agtagcagaa gctttactta ttccgcggta ctggacgata
     4261 gaaacccctt tagtagagga tgcaccgact atgctatttg aagacctagt gaagattgca
     4321 ggatatgaac tgcattatca agaaaacatg gaaacttatg aaaacgaata ttggttaacc
     4381 aaattagaga actaaaaagg agaaaaaaat atggctcaaa attactatga cgaatttgtc
     4441 aaattacctt tggataaaat ggcacaaaaa atggaggata tgacattcct atacaatgag
     4501 acacgtgttc caaaaaaaca ttataaggaa agattgtcgg tagctgtgga agaaatgatt
     4561 gaatcgggtg tcgaaatgaa tctcattgcg acctactatc gaacgcttga agaactgaaa
     4621 aagcaaaatg gacaatggtt tttccaagcc ttactatgct tggaagcagg cgtcaagcca
     4681 aacactatca aaccttctga ataccaagct ttagaactaa cgtatgggaa atttgtcgaa
     4741 acgaaaaaag ccaagactgt ttcaagtgaa tggttggact acttagaaaa tgtcaacaag
     4801 tatggcgcat gttacatcat gaaaaaggag gacaacgaaa atgaataagg tgatttatca
     4861 actaacaacg gaagatgtgt cacacttttt aacaaacaaa gaacatttga atgaagtaat
     4921 cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta ttcaaagagt
     4981 cgaagaacaa ttgtatttgg aaagtgaaac gagtcaaacg aaggttacag tcagcaataa
     5041 ttatttagac gtgtataaac gtcgtcctga cacattaaaa aaaggagcgg agcttctacg
     5101 actagaaaca gaaaaatata tcatgaatgt ttacctagaa aggagtgaac tggtcgaaaa
     5161 ctttgtcagc tacacgattt gcaggaaaca ctattactca gaatcagaat attattcaat
     5221 ttttgagagc atggaattaa tggaagagct aaacagcaaa gatgcgacgg tagacgatta
     5281 ttaccttgta tttactaaat ttttgacgtg tgggacggaa tctatcgtgt taatgggaat
     5341 ggattatgaa ctcgctgtac ccgaagtatt tttgagggaa tatgtagaag aagtagataa
     5401 cccaggcgac gactggtaca cgactttaac tgatttttta gatacctaca cttacgacga
     5461 tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac gatttgaaag
     5521 gaaaaaaggt caataaccat ttataccgaa aagtgagaat gtgtgaaata aaaacaaaaa
     5581 accttgaaat taagggaatg gttcttattt cgcaccaatc gctccaaccg cccccgccgc
     5641 ttaggtggtg cgaaataagc gagcaaagcc cgaaaaccta agagaaagaa ggagatttat
     5701 atgaacaaaa ttattccctt attattagtc ctatttgttg gagaaatact tatgcgactt
     5761 agtgcagctg tacatttaaa cctcgcagta tttttggtaa tactaatgtt ttgtttgatc
     5821 ttttggcgat ttgtattagc cctcgtaagt ctactattca agatgattgc agccatattt
     5881 ttcataggat tatttcgatg aaaggagaga gtaccaatgc caattgcaat aggaaataaa
     5941 cggttacccg tgacgttgga tgaaaagaga caaaaagaat tgcagtaacc aaagcagaag
     6001 tacggcaaaa gtgaatccag gattatgtgt attgcgttag atttattgat tgcgcaagaa
     6061 aaagcaggat ttgacatacc agcactcaaa aagtgatcgc actttgcgca cgtgttaata
     6121 cgtgttataa ttattttgta aggaggtata gtgcacaatg ccacttacgg gaaaagaaat
     6181 gcttaagtta ttgaaaaaaa tggctgggtt gagcgaaggc aagagggatt tcatcatcat
     6241 ctttataagg atggtgttag aatcactgtt cctgttcatg ccaatcaaga tcttggccga
     6301 ggacttgagc gaaaaatttt aaaggatgcg ggattgaaat aatctcgcat tcgattaaag
     6361 aaaggattga ctgaaatgtt aagtgagcct ttagcaaaaa cctatcctgc tatttttagc
     6421 ccagaagaag gtggtgggta ctttattgaa tttcctgatg ttcaaggtgc ttacacagga
     6481 attaacgaag acgatatatc ttatggaatt gctatggcac aagaggtgtt aggtatggtt
     6541 ttagcagatt atattgagca tgaagatcta cttccagaac caacaccaat caataagatt
     6601 tctgttgaag atgattcttt tactacttta attcgtgtag atgttgccaa atatttgaaa
     6661 gacacagaac ttgtcaaaaa aaccttaaca attcctcaat gggcggataa gctaggcaaa
     6721 agggctggca taaatttttc agtccttcta acagaatcaa ttgcagataa agcagataac
     6781 attcttcgtt ctggaagaaa taattagaac tcaaaagggt cagaaactta aaaatctttg
     6841 gcttcttttt ttactacaaa gatggcttct ttaaattatt tgcctgattg atttaaaaaa
     6901 attgcgcaaa gactacgagc taattaagtt gctcgtggtc tttttttatg aaaacttttt
     6961 agatc
//
LOCUS       DAFGFZ010000135         6962 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000135 DAFGFZ010000000
VERSION     DAFGFZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.137"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            274..870
                     /locus_tag="LR726_002583"
     CDS             274..870
                     /locus_tag="LR726_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL6217617.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(928..1410)
                     /locus_tag="LR726_002584"
     CDS             complement(928..1410)
                     /locus_tag="LR726_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217618.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1516..1713)
                     /locus_tag="LR726_002585"
     CDS             complement(1516..1713)
                     /locus_tag="LR726_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217619.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2008..2766
                     /locus_tag="LR726_002586"
     CDS             2008..2766
                     /locus_tag="LR726_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL6217620.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2826..3254
                     /locus_tag="LR726_002587"
     CDS             2826..3254
                     /locus_tag="LR726_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217621.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3300..3372
                     /locus_tag="LR726_002588"
     tRNA            3300..3372
                     /locus_tag="LR726_002588"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3333..3335,aa:Gln,seq:ctg)
     gene            complement(3522..3893)
                     /locus_tag="LR726_002589"
     CDS             complement(3522..3893)
                     /locus_tag="LR726_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL6217622.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4108..4293
                     /locus_tag="LR726_002590"
     CDS             4108..4293
                     /locus_tag="LR726_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217623.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4376..4564)
                     /locus_tag="LR726_002591"
     CDS             complement(4376..4564)
                     /locus_tag="LR726_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217624.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4588..4851)
                     /locus_tag="LR726_002592"
     CDS             complement(4588..4851)
                     /locus_tag="LR726_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217625.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5009..5266
                     /locus_tag="LR726_002593"
     CDS             5009..5266
                     /locus_tag="LR726_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL6217626.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5337..5528)
                     /locus_tag="LR726_002594"
     CDS             complement(5337..5528)
                     /locus_tag="LR726_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217627.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5669..5989)
                     /locus_tag="LR726_002595"
     CDS             complement(5669..5989)
                     /locus_tag="LR726_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217628.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6012..6446)
                     /locus_tag="LR726_002596"
     CDS             complement(6012..6446)
                     /locus_tag="LR726_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL6217629.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6781..>6962
                     /locus_tag="LR726_002597"
     CDS             6781..>6962
                     /locus_tag="LR726_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL6217630.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2176 a         1359 c         1068 g         2359 t
ORIGIN      
        1 tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat atatcagctg
       61 tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa ctaagaaaat
      121 ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat tttttgtgag
      181 catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa
      241 atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga cactttgaaa
      301 aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga agaattaaca
      361 actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca atcaacacgc
      421 gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga agaagcactt
      481 cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc tggtttcaaa
      541 aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg tctacaagaa
      601 caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa cggaatcgca
      661 acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt gcaacattac
      721 aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa ctggaaacta
      781 cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga atacatgaac
      841 gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac agaggttgtg
      901 acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat caatctctgc
      961 ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg cagaaaattt
     1021 cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt tagaaccttt
     1081 tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt tgtcaaagct
     1141 attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac taataaaaat
     1201 cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga tgagtgtata
     1261 ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat ttcgcacagt
     1321 ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa caacgaataa
     1381 aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt cttctccaga
     1441 aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag cacgccgcac
     1501 gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc ttgctttttc
     1561 atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt
     1621 catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata
     1681 ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact gctggaactt
     1741 ttctccacat taccactgct atttctttgg agatttaact cattgatttt tatttacagt
     1801 taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat taaaacagct
     1861 gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa actaattatt
     1921 tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat aataggaaag
     1981 ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa actattgttt
     2041 ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca tatccattct
     2101 ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa aaagacaaaa
     2161 gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc tccttcttta
     2221 gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg ttatgaaaca
     2281 actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc agatgggact
     2341 catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg ggctatggtc
     2401 gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga ttatcacact
     2461 tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa aggaaatcct
     2521 gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt tatgacttgg
     2581 accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt agtcactgga
     2641 tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg agcagtaccg
     2701 atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac tttggagaaa
     2761 agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg aagggagcgt
     2821 agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg attatttttg
     2881 tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg aacgagcgtc
     2941 gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct cttgtttatt
     3001 acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt gctcgaacag
     3061 agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt atgaatgaac
     3121 ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg attgcccttc
     3181 ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa caactatccg
     3241 atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg ttgaataaat
     3301 gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta ggttcgaacc
     3361 ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa gaacaatctt
     3421 tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat aattaaatta
     3481 tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat aattgctaat
     3541 gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg tgctccttta
     3601 ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa ttcaaccgtt
     3661 cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc atattcattt
     3721 acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa tggatttagc
     3781 tgatcaagta taccagggat ttctccataa cttccttcag tataatattt cagtccaaat
     3841 agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt catctttcta
     3901 cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg tcccaagttg
     3961 gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa aatgatcgtt
     4021 atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat gtaaaaaatc
     4081 atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca aaaagtcaag
     4141 gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga acataaagaa
     4201 caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa tatcgacagt
     4261 gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt gtaaaatcaa
     4321 aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact tcatactaaa
     4381 ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct tttcccacca
     4441 catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat tcttctcttt
     4501 ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt tcatatggat
     4561 tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt catatgcttt
     4621 tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt ctactgggat
     4681 actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg ctaaaatacc
     4741 atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat agtaaatcac
     4801 ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca tgaaaatccc
     4861 actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat cgatacagat
     4921 tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt attttgtatc
     4981 atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg ttttttatat
     5041 tccttttttt attatgataa gcattctttt ctattacata aaatggacaa aaaagaagtt
     5101 ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt tttcttttag
     5161 gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc tttcagtaat
     5221 ttttcttatt atttggatct attttttata taagaatcag aattgaatcc ctcgcatgtt
     5281 taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg ttttttctat
     5341 tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt taattctata
     5401 aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc gatcaggctt
     5461 ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa tacttttcct
     5521 gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt aagttatacg
     5581 cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga actaatcagt
     5641 tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc tgctcttctg
     5701 ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag ctgtataatt
     5761 ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct gtgacaggga
     5821 atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt tttcctttcc
     5881 actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca ataattgcta
     5941 aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt ttctattcct
     6001 cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac gacataagaa
     6061 gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc cataacgatc
     6121 aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg atctccagtt
     6181 tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat ttttttcagc
     6241 atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc ataggcacta
     6301 tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc attgtccttt
     6361 gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc aactgctacc
     6421 atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta ttgtaacatc
     6481 tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac aaaagcaatt
     6541 tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa tcccattatt
     6601 tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat tctgttacaa
     6661 aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat tttcacgtgt
     6721 taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag gagatttttc
     6781 atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc tgcattcttc
     6841 atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga tactctatca
     6901 acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc agaatcaaat
     6961 tc
//
LOCUS       DAFGFZ010000136         6735 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000136 DAFGFZ010000000
VERSION     DAFGFZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.138"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            15..548
                     /locus_tag="LR726_002598"
     CDS             15..548
                     /locus_tag="LR726_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217631.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(674..1351)
                     /gene="nth"
                     /locus_tag="LR726_002599"
     CDS             complement(674..1351)
                     /gene="nth"
                     /locus_tag="LR726_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL6217632.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1358..2056)
                     /locus_tag="LR726_002600"
     CDS             complement(1358..2056)
                     /locus_tag="LR726_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL6217633.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2324..3508
                     /locus_tag="LR726_002601"
     CDS             2324..3508
                     /locus_tag="LR726_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL6217634.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3514..3957)
                     /locus_tag="LR726_002602"
     CDS             complement(3514..3957)
                     /locus_tag="LR726_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL6217635.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4183..4470)
                     /locus_tag="LR726_002603"
     CDS             complement(4183..4470)
                     /locus_tag="LR726_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217636.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4641..5993)
                     /locus_tag="LR726_002604"
     CDS             complement(4641..5993)
                     /locus_tag="LR726_002604"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL6217637.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6266..6712)
                     /locus_tag="LR726_002605"
     CDS             complement(6266..6712)
                     /locus_tag="LR726_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL6217638.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1831 a         1321 c         1104 g         2479 t
ORIGIN      
        1 aaaaaataca atatatggtt ttgaataatt ttttgaaaga aggcttcatt ttgagttatc
       61 tgatagtatg cttaattatc ataatttgtc tgcaccatct ttttttatcg aaaacaatac
      121 ctcgttattc caagaataaa aaagctgaaa aattttgttt attattaaat aaatttactt
      181 taattgctcc tatccttgca tttattattt tttctgtact tttaagcact actcttaaag
      241 gaaaatttat ggaaagaagc tctcatgcaa tgattctcac ttttttatgg ttgttgttca
      301 ccagaatcta tatttttctg atgagcctga aaccgcccaa atccattagt ttatgtttag
      361 ttataaatgg tattttttta ttatcactta tcatttttat tacaccttta gatagatacg
      421 tcacttattt atataatcct ttggaatact ggacttattt tataggtata ttggaaggca
      481 ttattttcta catcgggtat tttcccaata aaaataacaa cttttatttt tatcgcaata
      541 aattgtaata agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa
      601 agataaacga aacagctttt ctccataata acaagataat tcatacattt atttgcaatt
      661 aaagaaacca cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc
      721 gatttttccc atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg
      781 ccgtgcaatg atatctacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt
      841 gtggtacttt ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga
      901 tcctcaggcg ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga
      961 atgcatcccc caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt
     1021 cttctcttga cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt
     1081 tgatgttttt tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat
     1141 cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg
     1201 ctttattcac tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa
     1261 atggattatt gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta
     1321 gagcttccat tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca
     1381 gtgagacttt cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct
     1441 gctgttttct tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt
     1501 cccacgatag caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt
     1561 cccgcagtgc tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga
     1621 tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca
     1681 ctgactggat ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat
     1741 actcacctaa tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga
     1801 caatcgtcgt ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa
     1861 tctgatcttg tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc
     1921 cttcaagctg tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga
     1981 tcctatgata atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat
     2041 tttctaaatt caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg
     2101 gaagcaagaa caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt
     2161 ctacttgatt tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa
     2221 attaagtaga tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta
     2281 tttttgtttc caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa
     2341 taaacattct aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat
     2401 cgtcagtcct gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc
     2461 tttctatatc actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat
     2521 tctcggtagc ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg
     2581 atcaagtatc ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg
     2641 tcgtatcatt gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga
     2701 catcacggaa cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat
     2761 aggaacaacg ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc
     2821 tatatttatt ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct
     2881 agaaagcctt ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt
     2941 tcaccaatta caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt
     3001 tgctgtttgg ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc
     3061 ctttcagtgg aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac
     3121 tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac
     3181 aagaataggg atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct
     3241 tctactgccg attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt
     3301 tactcctgta ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt
     3361 aatgggcggt acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg
     3421 acagctttat gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt
     3481 tgcctattat cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct
     3541 gtatcgaccg ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac
     3601 agtagcatct cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg
     3661 tccgattggc gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt
     3721 tatgatccct ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta
     3781 actagcattc gctaattttg tcacgttagg atcagggatt ttcccgatat gttcaatcac
     3841 ttctagttca tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg
     3901 aataatctca ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc
     3961 tacaccttct ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaaa
     4021 tattttgtag gaaaacccaa tctgaaacca agaaaaaaag gggttgacaa agcttgtcat
     4081 aacccctttt tctgattaaa agatagtaaa gagacagtca tcggtaattg tgatgatttt
     4141 ttgtcatcac ttaaggatga gcggcctttt caaaaactgt ctttaaagat catcatcagt
     4201 ttctagcaca tatggctgac cttcatatgt gtactctgta ttcaatgtgc cgtcattttc
     4261 atctgagtag atgcgtctac tgaaaggaaa acgatgagaa acattatctt tggcgtcatt
     4321 tgctggcatc gtgtaatatt cctcttgcgt ttcattcaac tgagagagta cttgatctgc
     4381 caactcatca atattgatcg ttaatttctt cgaagcactt ttataatctt ctaaaaatgc
     4441 atcttgttgg acaaaatatt ctttattcat cttctttctc acctttcttt tcaaaatcaa
     4501 ataaagctgc ttgttctttt atgtaacagc tttatctttg ctgcttattg tgacaaaaaa
     4561 ttcgaagaaa gacaactaat tgtacttttc acaaaaaaag acagagggtc gtgacaaaat
     4621 tgtgtcacaa ccctttttct ttattgtgca gtttcgattt cgcctagcgc tgcttgtgca
     4681 agtaggttca agtaattcca tgggcggtca aaatgtggtt ggaagaagaa atctgaaagt
     4741 gcgagatctt ctacagtcat tttattttga acagctaaag acatggtatt tgctgattgt
     4801 gtaatgtcgt atttggacat gaactgagca cctacgatac gattcgttcc tttttcatag
     4861 actagctcca tcattacttt ttcagtagtt ggcatgaact ctggacgata attatcttca
     4921 aatacagtcg cttggacttc tagatcgttc aaagttgcgc tttctttggt cacacctgtc
     4981 gaaccgatcg tccagccaaa taggtaaagt cctgaagttc cttgagtacc acgatacgcc
     5041 attttatgat ctgtcaagtt atttcccaca agcaaacctt gacgaacggc atttgttgcc
     5101 aaaggaatat agttttgcgt gccacttgga ttgtagttga ctactgcaga gtctccagct
     5161 gcaaaaatgt ctggattact tgtctgcatg tattcattca ctttgattgc cccgtttggc
     5221 agtgtatcta cttttccttc gatcagtttt gtatttggac ggaagcccac acataaaatg
     5281 accatatctg tatcaaatgt atctgttggt gtagctactt gtttcacttt tccttcttca
     5341 tctgtgataa attcagtaac attttcgcct agcgcaagag taacaccgcg gtctgtcaat
     5401 tctttttcta agatatctgt gaatggttta tctagatatt tattcaagat gcgatctaat
     5461 ccatcaatca atgtcacttg ttttccagat tcgacaaaag cttctactaa ttcaataccg
     5521 atatatccac caccaacaat tactacacgt tttgcttctt tcgcttgtgc aataatttca
     5581 tttgcttgat tgtagttttt gcataggagt acatttttcg catcgattcc actaatcggt
     5641 gggataatag gccatgaacc agttgtcatc actaatttat cataagaaac cgtttctgtt
     5701 tctcctgttt tcagatcttt tgccgttact gttttatttt ctgtatcgac gtctgttaca
     5761 tcgtgttcca ttttaactgt tgctcctaat gaagataatt cttcaggatt tgagtagaac
     5821 aagcctgctg ggtcctttac tactccgccg acataaagtg cgataccgca agataagaaa
     5881 gaaatattgt catttctttc atatactgtc acttctgctt ctggatgatt ttttaaaata
     5941 ctttttacag ctgcagtacc tgcatgagta cagccaatta ctactacttt catttatggt
     6001 ccctccgatt aacattttgt ccttcgacaa aactactata acagaaataa aacggataca
     6061 acagtttaat gcttgtgaat tcacaaaggt ttgtttgtag tttcattcct tttcaagcat
     6121 aagaacccaa tatctacaag attcttattt taataatatt cacttttata tctaagattg
     6181 tgaataaaag acaaaaaaat aaaaaagcta gcgttttttt tacacagcta gcttttttta
     6241 ggtataacac ttgtagaaac tgtttttaca tttgcgcctt ttttgctgac tctttacaat
     6301 ccggacagag tccatacaat tccaaccgat gttcattaat ctcaaaatca gtcagtctgc
     6361 ttgcagccat ttctatatct tcaagccctg ggtaatgaaa atcaacaatc ttaccgcagt
     6421 tttcacaaat tacatgataa tgacgctttg aactgaaatc aaatcggcta gaagcatcgc
     6481 cgtacttcat ttcttgaaca aatccaatcg aagtaaataa gcgaaggtta ttgtaaacag
     6541 tcgcaacact catatttgga aaccgatgtt caagtgcttt ataaatctca tcagccgttg
     6601 gatgggaatg atgttcaatc aaatattcta aaaccgcgta ccgttgaggt gtgattcgaa
     6661 tgttctcttt ttttaacttt tcgataacct cttcgactaa cttattttcc atgtaaacac
     6721 cccacgttgt tattc
//
LOCUS       DAFGFZ010000137         6714 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000137 DAFGFZ010000000
VERSION     DAFGFZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.139"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..631)
                     /locus_tag="LR726_002606"
     CDS             complement(<1..631)
                     /locus_tag="LR726_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217639.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYK"
     gene            complement(751..1398)
                     /locus_tag="LR726_002607"
     CDS             complement(751..1398)
                     /locus_tag="LR726_002607"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBL6217640.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1482..2909)
                     /gene="uxaC"
                     /locus_tag="LR726_002608"
     CDS             complement(1482..2909)
                     /gene="uxaC"
                     /locus_tag="LR726_002608"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL6217641.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(2983..4062)
                     /gene="uxuA"
                     /locus_tag="LR726_002609"
     CDS             complement(2983..4062)
                     /gene="uxuA"
                     /locus_tag="LR726_002609"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL6217642.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4065..5696)
                     /locus_tag="LR726_002610"
     CDS             complement(4065..5696)
                     /locus_tag="LR726_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL6217643.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6000..6671
                     /locus_tag="LR726_002611"
     CDS             6000..6671
                     /locus_tag="LR726_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6217644.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2166 a         1348 c          922 g         2278 t
ORIGIN      
        1 gtttataaca tattaaataa caaataagtg cacatatcga aaatattccc gcaacaattg
       61 taaaagttga tccaccacga actatttgag cgcctgaatc atctcttgta taaataaata
      121 atggtgttaa tacaccaatg accaaacttg ctaaagtagc tccaacattt cggaatgtcg
      181 atagcattgt tctttcttct gtaatagaag taatagctga tgccattgat ccataaggaa
      241 tatttataga tgtgtaaaaa atactccccc ataatatata cgtaacatac atgtatacta
      301 ccttaacgct catagaagct gaaatcatag aagtttgata cattaaaaaa cttgctaaag
      361 cgactggacc actcattctt aaaatccacg gtttaaactt accctgctta gttgctttcg
      421 atctatcaac aatacgcccc atagttacat cagtaactgc atcgataaat cttgctacta
      481 aaaataatgt tccaacggca gctccgctta tccccaatac ttttgtataa aagatcatta
      541 aaaaagaact agcaaaaatg aaagtaaaat cgtttccaaa atctccaaac atatatccta
      601 ttttgtctct tataccaaat tttctttcca tacaattctc tcccttctgt tgttaagcgt
      661 tttcattctg atgaaagtac gtactatact ctgaagcgta aagagtcagt atttattttt
      721 tacatttcag agtattattt gttttttctc ctaataattc tttatctcat aaaacttatc
      781 tatatattta ctagctactc gacttacttc ttcaaaatca cctttttcaa cagaagccaa
      841 taaattccca cctacaccaa ctgcaacaac tcctacttca aaccaatctt ctaagttctc
      901 tatacttact ccgccagtag gcattatatt tacgtgtggt aatggagctt gataggcttt
      961 tacaatggat ggaccaaaag cagaacctgg gaatagttta atgatatcta cgccagcagt
     1021 aagtgcctcc tttatctcag taattgtcat acaacctggt aaataaggaa tttgatataa
     1081 attacaaatc tcagctgttt gtcgatcaaa agatggacta acgatatatt ctgccccagc
     1141 cataattgcg atacgagcag taaacgcatc taaaaccgta ccggctccga ttattacatc
     1201 tttttgatca gcatattttt tagcaatttc tttgattatt tcttcagcat taggaactgt
     1261 aaaagttagt tcaatccctt taattccacc cgcaataatt gaatttattg ctttaatggc
     1321 ctcatcctta tttttcccac gtacaaccgc aatgactcca gccgcttcta acttacttaa
     1381 aatttctact tttttcatat ctatctcctt cctttatgga tacatacttt gtctagctgt
     1441 actaacttac aaagaataaa tttaagcttt tatttcaagt attattttct acttccgatt
     1501 tcaaaaaaat taaaataatt atacgcatta cgataacaaa tattctccac tatctgacta
     1561 agatacattt catcatctgg aattcggcct gctttaaccc actcgcttag taattgacat
     1621 aatactcgac gaaaatactc atggcgagga tagcttagaa aacttcgaga atcagtcaac
     1681 atacctgtaa aattcgacaa caaactttgt tgtgccatcg tagtcaactg ttgacgcatt
     1741 ccttcagctg tatcattgaa ccaccaagca catcccaatt ggattttttg aatcataccc
     1801 ccttggaagc tttgcattgc tgttgctagt ggaagccaat cagttggatt taatgaatat
     1861 aaaattgttt ttggtaagca cccactcttt tcagcggtat cgaacaatgc tacaacattt
     1921 tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga catctactcc aatttgttcc
     1981 tttaatatac ttgaattatt tcttaaagca tttaaatgca tttgcatcgt ccaaccatat
     2041 cttgtatact cagacatcaa atgtaattga atacctgtaa caaattgtgc aatttctgat
     2101 aaatggaaat ccgtttttcc tgctaaagct tttattaaca gattgtcgag tattttttca
     2161 tctgctattt cataaaaata agtattcaaa ccgtgatctg ctaatcgaca gccaatttga
     2221 tgaaaatgat ccactcgttg gctcaatgct tgcttcaacc cattaaagtc tttgattggt
     2281 atagccgctg tttgactaag ttttaaaata tagttcgaaa agcctgatga tttgatactc
     2341 cagaccttat ctggacggaa agtaggtaat accttgaaag aattatcctg tttagaaagc
     2401 tcagtatgat accacaagtc atctgtagga tcatccgttg tacagatggc tttcacatcc
     2461 atcatctcca acaattcttt cgggcgatag gaatccgttt gaattttttc gttagcacgt
     2521 cgccaaattt ctggtgcatt cttctcagaa attgtcaaat caatatcaaa gtaacgttgt
     2581 aattccaaat gagaccattc aaaaatagga ttacctggcg ccttctccac tgtttttaca
     2641 aaagctaaaa atttttcata atcatcacct ggaccactga tcaactcttc ttctatacca
     2701 ttagctcgca tcaatcgcca tttataatga tctccagctc ctttatcatt taaccatacc
     2761 tgagtcaaat tcttgtattg cttattttct agcaaatatg ctgggtctaa atgacaatga
     2821 tagtcgataa ttggttggtg ttttgcaaat ttatgaaaaa gtcttttagc tatttcattc
     2881 tctaataaaa agttcttatc taaaaacata tttaaacact cctttccttc cgttagtttt
     2941 tagaagagaa aatttttcct ctaaataaac tcccttatat aattatttac tcgccccctt
     3001 ttcaagcgct tcccaaatac catttaaata aactgcacct agagctcggt catataatcc
     3061 ataaccaggt ctaccggttt ctccccaaat catccgacca tgatctggac gaatatagcc
     3121 attaaatcca ttatcgtaca ttgtttttaa tatcttatac atatctagag aaccatattc
     3181 tgatttatgc ggcgcttcgt aaaaatcgag tccattgtca cttaaatatt tgatattacg
     3241 aacatgtgca aaaggaatcc gatcccgttg acaaaattca gatagaattt catagacatc
     3301 attatctggt cgttctgcaa tacttccagt acataatgta ataccattag aaggagaatc
     3361 cactacatta cacaaccaat ctaaatcgtc acgattttta acaatacgag gtaatccgaa
     3421 aatggaatat ggtggatcat ctgggtgaac tgccatttta atcccaactt cttcacatgt
     3481 cgggataatt gctttgagaa aatatgcaaa gttctctcgt aatttctctt catcaacttc
     3541 tttataggct tctaataatt gcttaatttg tgataaccga tgaggttccc aacccggcaa
     3601 ttcaaaatta ttagtagaat tttctacttc tctaataatt tcctccggat cagtagggat
     3661 attttttgaa atataggcta acgtagaaga cccatcatct aaacgataat ccaaatccga
     3721 ttttacccaa tcaaatactg gcataaaatt ataacagata acctcaatcc caaattctgc
     3781 caaattgcga atcgtctgtt tataattctc aatgtattga tctcgtgttg gtaaaccaat
     3841 tttgatatca tcatgaacat taacactctc aataactttc aaggtaagtc cattggcttc
     3901 gacttcatct tttaaagatt taattcgttc tctcggccaa acctctccgg ctggtatatc
     3961 cattaatgtt ccaacaattc ctttcattgc tgggatctga cgaatttctt gtagtgagat
     4021 tttttcttca tttgccccat accatctaaa agtcatttcc atagttactt tcctccaact
     4081 tctgttatat atttattgat tgttttacga actgcttgag gacctactag catttcgcaa
     4141 aagatttttt cagttttctt tcctacccca acttgatata aatctaaacc aaagatttct
     4201 ttattttcta aaatttcctg taccgcacaa tgaatctttg aaggatcttg ttcacctaat
     4261 ttcaatactg ataatttatc ctgcaactct accaataacg gatcaggact tggcacaaaa
     4321 ttttcccctt gatcatcgac tgccagtaaa taacgtaacc aagcagcaat agcgatagga
     4381 ataaatgtta gattttctgc atttccgtct ttttcgacat actttttaat tgtttcacca
     4441 tagcgaatcg ctactttttg agaagtatct gttgcaattc gttgtggtgt atctggaata
     4501 tagggattag gcaaacgttt attaattact tcatcaataa attgttttgg atcaataatg
     4561 cctggatttt caactactgg taaaccttct aaataaccta ttcctttgat taaaccaacc
     4621 aaatcatcat cctgcatctc tgctgaaatt gattgatatc ctagcagaca acctgttact
     4681 gccaaagcag tatgaagtgg attcaagctt gtcgtaacct tcatgatgtc tgctttatca
     4741 accgtctctc tatctgtcat aatcacacca gccttttcca atttaggccg gccatttgga
     4801 aaactatctt caataactaa ataatgaact acctcagtat tagtgaaagc agcaatattg
     4861 gtatgttttt ttgtgtgaat gatatctaaa tcctcgagcc ccatcatcaa tagatcttcc
     4921 tttacattct ctgaaggatt tggcgtaatt cgatcaatca tactccaagg aaaagtcact
     4981 ttttctttat cctttaaata agcaataaat ttttcagaaa catatccttc tttttgccaa
     5041 gcttctgcaa tcgtaaaaat actatcttga aaacgttgac cattctgtga gaaattatca
     5101 gtagaaacca tcgcaatcgg caactctcca gcattaaatc tgctatataa caaggatgtt
     5161 acaatactca tcgtatgttg aggattgcta ggtccagatt tgatatcagc tttgactacc
     5221 gaaaagaatt gccctttaga atcttttaaa ctgtaacctt tttcagtaat agtaaacgtt
     5281 gtaaactgta atttaggatc ttcaaaatat ttaaccactc tttgatagtc cgctatattc
     5341 ttcggattgc aatataaact atcagccgtt actgccaaaa gtcttttttt gagttcacca
     5401 tctccactca taacaatttc caaaatgtca ttgttatagt catgataagc tttttccact
     5461 acttcttcat caaatgtttc acacacaact actcctgttt ctaattcatt ttgatctgct
     5521 aagcattgag caatttctgc atgaaatcct cgataaagat ttcctccacc aaaatgaagc
     5581 catactggat gttgctggcc ttcttgctta acttctttga catcaaaaca cggcagtaga
     5641 atctgcattt cattgaattc ttcgatagaa ttttgccaat ttttaatcga aaccataatt
     5701 aacctcctca aactgatatg ttagttttga tacattttat attttaaaac ccattgtaac
     5761 cgttgtcaat ataaaaaggg aataaacttg cttttttcta ccatatcaga atgattgtat
     5821 tttttattca gaaaaataaa atactccttc tttttggact gattttatcg aaacttattt
     5881 tttctttttt tcatgtattc ttttattttt ataactagtg tattacttca aaatttgata
     5941 ttatatggca tgaacagaat taaaatatca gttaaaataa aagttaagga gcattgtgta
     6001 tgacaattaa aactagtctc caaaatcaag cttaccaatc tattcgccga caaattatct
     6061 atgctgactt agaacctgga aaaaaagtat cagaaaaaaa attagaagaa acactttcta
     6121 ttggtcgtac accaatacgt gaagcactga ttcaactccg acaacaaggt cttgttgata
     6181 caattcctca aagcggaaat tatatctcat taattgattt agattcggcc aaaaatgctc
     6241 gatttgtcag agaacatttg gaacgacaaa ttatgttaga gtgttgtgcc aaaatgaata
     6301 accaaaaaaa acggatttta gaaactataa tttcagaaca agaaaaagct gttagtcaaa
     6361 aaaatgctcg tagttttttt cataatgata atttattcca taaagcttgt tttgaaattg
     6421 cagagcgaga agaagtatgg caatggttag aaaataacaa tacacactta gaacgatttc
     6481 gatggctacg agtaaaaacg gaaggattaa agtgggaaaa aattatggaa caacattatc
     6541 aactatttga ggcattaaca aaacaagatc cagaagaagc taactttcta acatcgttgc
     6601 atcttcattt aatgcttaat gagcaaaata ttgttttagc tactcatcca aaatatttca
     6661 aaaatatata acttttaact ctttttaaat taaatggttg ttctgatcag aaaa
//
LOCUS       DAFGFZ010000138         6595 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000138 DAFGFZ010000000
VERSION     DAFGFZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.140"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            81..1061
                     /locus_tag="LR726_002612"
     CDS             81..1061
                     /locus_tag="LR726_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217645.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1183..2601
                     /locus_tag="LR726_002613"
     CDS             1183..2601
                     /locus_tag="LR726_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBL6217646.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2672..4141
                     /locus_tag="LR726_002614"
     CDS             2672..4141
                     /locus_tag="LR726_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6217647.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4151..4618
                     /locus_tag="LR726_002615"
     CDS             4151..4618
                     /locus_tag="LR726_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL6217648.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4654..5526
                     /locus_tag="LR726_002616"
     CDS             4654..5526
                     /locus_tag="LR726_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL6217649.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5608..>6595
                     /locus_tag="LR726_002617"
     CDS             5608..>6595
                     /locus_tag="LR726_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL6217650.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLD"
BASE COUNT         2251 a          932 c         1355 g         2057 t
ORIGIN      
        1 gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc gagtaggtag attttctatc
       61 tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag agaagttgcg aaagaggcaa
      121 atgtttctcc tacgactgtg tcgagggtat tgaatgatcg tggatatttg agtgaggaaa
      181 caaaaagaaa agtttttgat gccatgaaaa aattagaata ctatcctaat gaactggcga
      241 gagcactttt taaaaacgaa acatataccg taggtttgat ttttcctaca attactaatc
      301 catttcacgc tgagttaatt caagatatcg agttcgagct ttccagcagg ggatataaag
      361 tgttgctgtg caatagctta aacaatccaa caaaagaaaa agagtacttg acgatgttga
      421 gaaagaatca ggttgatgga attatagtag gaacgcacaa taaagatatt gcggattatg
      481 atattccagg gctacctatt gtagcgattg atagaagtct tggtgaaaat acagttacag
      541 tatcttgtga taattacagg ggtggggaat tagctactca actacttgta aatcatggct
      601 gtcagaatat actgtgtatt cggggagata gtaagataaa gctacccgcg aatagtagga
      661 ctttagcata taaagatgtt ttgaataaat tcatgctgga accgttagtc ctagaagtac
      721 catttgtaaa agaaatcaat gaaaaaaatg aaataattaa gacatatctg gaaactcatc
      781 caaatattga cggggttttt gcaggagatg atttattggc ttctttggcg attaacaact
      841 taggaagttc gggcaaaaga gttccaaaag aggttaaagt gattggattt gatggagctc
      901 aacaaacatt gatttataat ccagaattat caactattca acagcctata gatcaaatat
      961 ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg cgaaaaggag acggatgaac
     1021 tttcacttcc agtaaagctc ataaaaaaaa atacagcata agttaaatcg gcgaaaaatt
     1081 ccttcgtcga tttaaaaata tttttatgaa acgggttgac atatgaaacg ggttgacata
     1141 tattagttgt gaagataaaa accttttcac tggaggaaga aaatggagaa attattaatt
     1201 aaaagcaata atcatcaact ttactaccaa cctaaggatg tttgggtcgg agatgttatg
     1261 ccttacggga aagaaggaaa attctttatt tatcatcaac gtgatactag aaatccagtt
     1321 ccttttggag aaccgtttgg ctgggcttta gcaactacga aagattttgt tcattttgaa
     1381 gactttgggg aatctctttt aagaggggat gacaatgaga gagaccagtt tatctacgct
     1441 ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt atacagggta taatcgtgaa
     1501 ttcttagcgc agggaaagac atctcaagtg ttgcttcatg ctgagagtga tgattttgtt
     1561 cattgggaaa aatcagaaac agcattggag ttagaaccac aagaagggta tgatattcga
     1621 aactggagag atccatttgt cgtttgggat gatgaaaaag aagaatattt gctaatttta
     1681 ggggcgcgaa agggtcagga taaacatcta caaaccggac gtttagtgga atttacatca
     1741 aaagatttaa ctaattggga attcaaaggt gatttttggg ctccgggatt gtataccatg
     1801 tttgaaatgc cagaactttt caagattggt gactggtggt atcttgtcta ttcagaatac
     1861 tcagatggaa ataaaatact ctatcgaatg agtcaaagtt tggaaggtcc ttggattaag
     1921 cctaaagatg aagcttttga tggacgggct tattatgcag gtagaacagc ttatgatgga
     1981 aaacgtcgag tcctatttgg atgggtacca acaaaagatc aagaaaatga taaaaacaat
     2041 tatttatggg gaggtacttt ggttcctcat gaagtctatc aaaaagagga tttttcactt
     2101 ggggtaaaac ccatagacga agtgtggaat gcattcataa atttacatga tattgccgat
     2161 gtagagatag aaacagcaga cacaagagaa gaagtaacgc ttgtcaaaaa tagtggaact
     2221 ttgtttaatt tagaaacaac aatttcattt gatgagaatc ttagtgactt ttcactgaga
     2281 ttatataaaa accttgagac agatgaatct tacgaatatc gcttcaattt agatgagggg
     2341 cgtcttacat ttgataaaaa cccttgttac ccttggtttc aatatatgga taaaggactt
     2401 attagaccta tatctttaaa gcctaaccaa tcttataaac taaaagtgat tgttgatgac
     2461 aatataatga cactttatat tgatggaatc gctcttaacg ctcgtgtaca tggttgtttt
     2521 ggtcaagcaa ttactattac agcaacaaat ggatcaatga ctgcaataaa taccaagttt
     2581 tcaaatgaaa taaagaaata ataaacgtca atcatgtggc aggtctgaaa atcaaacatg
     2641 acctgtcaca tttaaaaggg aggataaatg aatggactac aaaaaaattg ccgaagatgt
     2701 attggaaaac gttggaggaa aagagaatat ctcaagcgct acacactgca ttactcgttt
     2761 acgtttaatt cttaaagaca aaagcaaagc gaatatggaa atgcttgagg aaatagaagg
     2821 tgcaaaaggt gttcttttca atagtggaca gttacaagta attttcggaa caggtataat
     2881 tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct aaggaagtat ccgtttctga
     2941 aatgaaagac gaaggagtca ataattcagg aaatagactt caacgagctt ttaaagtttt
     3001 ctcggatatc tttattccga tcattccagc ttttattggg gcagcaatgg ttttaggaat
     3061 tcgttcgttg attaccactc ctggaatgtt tggaatggat ggctcattcg ctgatcacaa
     3121 tgtattccta aatgatttat ccaaatttct gggtattatt gcaaccacat ttgattattt
     3181 accagtttta gttatgtatt ctgcaacaaa acgttttggc ggaaacccta ttttaggatt
     3241 acttgtagga tttgttatga ttcatccagg actagccaat cggaatgatt tcgtcatggg
     3301 aactataaag cctgattatt ggcatttatt tggattaagc attccagcag ttgcgtttca
     3361 agggggtgtt tttccagcaa ttttgacttc ttggttttta gcaaaagttg agaaaattac
     3421 aaataaatat tctcctcaag tacttgctta cattttagtc ccaactgtga caattgttct
     3481 tgccaacttt gcactatttt tagtgtttgg tccattgggg aattttatcg gtaatgtttt
     3541 aggtggaatc attgatttcc tatatatgaa gatgggtgcc tttggtgctt ttgtttttgc
     3601 aggcgcgtta caaccattag ttgtaacagg aacacaacat gctattcaag gtattgaggc
     3661 aaacttagtc gcaacaactg gattcaatta tatccaacct atttggtcag tttcaattat
     3721 tgcccaaggt ggcggttgta ttggtatgta tttactcttt aagaaaaaat ctaaagatag
     3781 agatattgcg atttcaagct ttattccaac actggtaggt attactgaac cggctatctt
     3841 tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc tcgtttattg gtgctggttt
     3901 tggtggagca tttatgaaaa tttttgatgt aaaagccatt ggacaaggat tgactgtatt
     3961 gccaggtctg actattgcta atccagttct tccttatata tttggtaatc tcatagcatt
     4021 cattttacca atcattttta ttctcgctta tgataaatat aaagtgttta tccctgaagc
     4081 taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa ttgccagttc aagtaaagtg
     4141 agatgagagc atgttaaatt tttttagaaa aaatgaaata agcgttgtag cacctgcaac
     4201 cggtgttttt gaaaaactgg agaaattaag tgatccagtc ttttcaaaag gaatgatggg
     4261 acaaggattt gcaattgatg caaaaaacga aacgattgta tctccaatcg atggttttgt
     4321 ttcaagtatt tttcctacaa aacatgcctt aggaataaaa acaaaatcag gaattgagat
     4381 acttgttcat gtaggaatag atacagttga gctaaatggt gagggatttg atattaaagt
     4441 tcaagaagga caaaaagtta aggctgggga tattcttatg accattaatt ttgtagtttt
     4501 tgaaaagaat cacaagacta aagatgtcat tgttgttttt ccagactacc aagacgaaat
     4561 tttcattccg aaacttgaag aagtaaaaca aggacaagaa ttgtttgttc ttggctaatg
     4621 ataagtaatt aataatagaa tcgaggaaaa tgtatggttt ttggggctat tgaagctggt
     4681 ggaacaaaat ttgtttgtgc cattggagat aaagatttga acatatttga aaaagtcagc
     4741 tttcccacga ctacacccaa agaaacaatg aatttagttt tagatttttt taatcatcat
     4801 aaagatgatt tagaaggtat cggagtggga tcgttcggtc ctattgatat tcattgtgaa
     4861 tctaaaacgt atggcttcat tacatcaacg ccaaaacttg cttggcagga ttttgatttt
     4921 attggaagtt taaaaaagca acttaatatt ccaactgctt ggacgactga tgtaaatgcc
     4981 tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc tttcaagtgt tgtttactat
     5041 accgtaggaa cgggcatagg aggtggcgct atccaaaacg gaaacttcat tgaaggtttt
     5101 agtcatccag aaatgggaca tatgcttgta aaacgacaca ataaagacaa cttttctggg
     5161 acatgtccat tccacggaga ctgtttagag ggcttagctg ctggtccggc aatcgaaaga
     5221 agatcaggga aaaagggaca agaagtgact gaaaatgacg ttacttggga aatagaagct
     5281 tattacattg ctcagtgtgc atataataca acattgatgt tatcacctga tgtcattata
     5341 tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga aagtacaaga taagtttatt
     5401 gagttgatca acggatatgt cgctattccc gatgttgaca attatattat gacaccaaaa
     5461 ttgttagata attctggtat tgtaggttgt ttagctttag caagagacgt taaagaaaaa
     5521 tcttaatttg gaaaaaagat cgtctcgact atttgtttgg ttgatgcgat cttttttttc
     5581 aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa tgaaagatga agtattagag
     5641 tatatcaaaa ataatttaaa gtattacgat ttttctgctc aagatattgc aatgaaattt
     5701 tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt tattttcaga tggaaaatta
     5761 ctaaagaatg attctgttag accagtaatg ttcaagtata atcaacaaaa gccaaaagac
     5821 tgcttttcta agtttattgg agcagacata tctttgaaat ccactataga taaatgcaaa
     5881 gctactgtaa tgtatccccc taatggcctc ccattaataa taaaaggaaa cagtggcgtt
     5941 ggaaaaagtt ttttagcaag tttgatctat cagtatgctc ttgatagaaa agtaattcat
     6001 aatgacgcaa aatttgttgt tgtgaattgt gcagattatg caaataatcc agagctattg
     6061 tcagcagttt tatttggata taaaaaagga gcctttactg gggctgaaaa agatactgca
     6121 gggatgctat cttctgctaa tggaggatac ctttttttag atgaggttca caatctaagt
     6181 gcagaaaacc aagagaaatt gtttcttctt atggattctc aaaagtatcg taaacttggt
     6241 gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct tagccacaac agaagataaa
     6301 aatacctctt tgttagcaac ttttagaaga cgcatcccag ctgaaatcac tctgccagat
     6361 tacaataata gaagtacatc tgaaaaaatt caattattat ttgaatttct taaagatgaa
     6421 gcaattacaa ttgattcaaa aatattttgt tcgattgaac tttttactga tcttttatcg
     6481 aaaacatttg aaggaaatgt tggagaattg aggaatgaaa tcaagcttct ttgtgcagaa
     6541 ggttatttag gaaataagag aaagtcttca atttatttag gggacaaact agaca
//
LOCUS       DAFGFZ010000139         6578 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000139 DAFGFZ010000000
VERSION     DAFGFZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.141"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(142..468)
                     /locus_tag="LR726_002618"
     CDS             complement(142..468)
                     /locus_tag="LR726_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217651.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(505..870)
                     /locus_tag="LR726_002619"
     CDS             complement(505..870)
                     /locus_tag="LR726_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL6217652.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(907..1122)
                     /locus_tag="LR726_002620"
     CDS             complement(907..1122)
                     /locus_tag="LR726_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217653.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            1251..1586
                     /locus_tag="LR726_002621"
     CDS             1251..1586
                     /locus_tag="LR726_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217654.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1662..3659)
                     /locus_tag="LR726_002622"
     CDS             complement(1662..3659)
                     /locus_tag="LR726_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217655.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(3757..4032)
                     /locus_tag="LR726_002623"
     CDS             complement(3757..4032)
                     /locus_tag="LR726_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217656.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4264..4998
                     /locus_tag="LR726_002624"
     CDS             4264..4998
                     /locus_tag="LR726_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217657.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5037..6179
                     /locus_tag="LR726_002625"
     CDS             5037..6179
                     /locus_tag="LR726_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6217658.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2241 a         1198 c          960 g         2179 t
ORIGIN      
        1 acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc agactattat
       61 ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttgaac
      121 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac
      181 atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac ttccattgtc
      241 taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg tcacatcatc
      301 acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg gtacagatga
      361 cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa tagccaatac
      421 aacgatacct ataatgacaa acttcttatt catatttact ttattcaact ttttatctcc
      481 tatctccaaa atgattttct ctcatcagta actcattgac atccccaata gtacaccaaa
      541 gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttctctcg
      601 atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa atgctaatga
      661 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
      721 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc
      781 ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac tttcatttgt
      841 tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct
      901 tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta ttggtacact
      961 taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1021 cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt gggaaagtcc
     1081 tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc ccttctgttt
     1141 tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac ttcaaatgtc
     1201 attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta atgaaacaga
     1261 agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca caaaaagaat
     1321 tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
     1381 aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
     1441 ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat cgctctctta
     1501 gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg caagaaaatg
     1561 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
     1621 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tataattggg
     1681 tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgataaata
     1741 ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat ccctgcccct
     1801 ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt cttaatatcc
     1861 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttctattaaa
     1921 gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt gttaataagt
     1981 tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
     2041 tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg aggaaatact
     2101 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
     2161 gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
     2221 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa gtttcctaac
     2281 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
     2341 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
     2401 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
     2461 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
     2521 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
     2581 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
     2641 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
     2701 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
     2761 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
     2821 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
     2881 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
     2941 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
     3001 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
     3061 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
     3121 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
     3181 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
     3241 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
     3301 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
     3361 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
     3421 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
     3481 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
     3541 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
     3601 ttctctaata atttttcttt atctacataa attggaatat taatcattga tgtttccatt
     3661 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
     3721 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
     3781 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
     3841 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
     3901 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
     3961 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
     4021 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
     4081 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
     4141 atgattacca gttcatcgac atacacataa tacctcgata aaaagggggt gtcaatagtt
     4201 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
     4261 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
     4321 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
     4381 aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac ctatttacca
     4441 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
     4501 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
     4561 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
     4621 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
     4681 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
     4741 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
     4801 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
     4861 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
     4921 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
     4981 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttcatgg
     5041 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
     5101 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
     5161 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
     5221 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
     5281 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
     5341 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
     5401 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
     5461 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
     5521 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
     5581 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
     5641 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
     5701 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
     5761 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
     5821 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
     5881 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
     5941 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
     6001 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
     6061 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
     6121 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
     6181 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
     6241 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccaatgactc
     6301 ttttgggaat cagaggaaag aatcaggctt tatagagata tttcatctaa caaattactt
     6361 tagactgaca gtatatatca gcaggtactg tcatcgggaa cgtagccaat atcgtatcca
     6421 aaacagcagg tagagaagct atttgaggat tcaagttatt ctatttcaac catgaagtta
     6481 agttcattaa aaataaagat agttggtatt tcaataattt ttgtatagag ttaatcactt
     6541 tattgatgct aattcatctt gattggtatt ataatcgt
//
LOCUS       DAFGFZ010000140         6465 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000140 DAFGFZ010000000
VERSION     DAFGFZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.142"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..142)
                     /locus_tag="LR726_002626"
     CDS             complement(<1..142)
                     /locus_tag="LR726_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6217659.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="LR726_002627"
     CDS             complement(183..437)
                     /locus_tag="LR726_002627"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL6217660.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="LR726_002628"
     CDS             complement(548..802)
                     /locus_tag="LR726_002628"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6217661.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="LR726_002629"
     CDS             complement(995..1270)
                     /locus_tag="LR726_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBL6217662.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="LR726_002630"
     CDS             complement(1242..2195)
                     /locus_tag="LR726_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL6217663.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="LR726_002631"
     CDS             2807..4300
                     /locus_tag="LR726_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBL6217664.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="LR726_002632"
     CDS             4435..4731
                     /locus_tag="LR726_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL6217665.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="LR726_002633"
     CDS             complement(4755..5714)
                     /locus_tag="LR726_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL6217666.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..>6465
                     /locus_tag="LR726_002634"
     CDS             5897..>6465
                     /locus_tag="LR726_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217667.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGY"
BASE COUNT         2071 a         1089 c         1135 g         2170 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatga
//
LOCUS       DAFGFZ010000141         5941 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000141 DAFGFZ010000000
VERSION     DAFGFZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.143"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(167..625)
                     /locus_tag="LR726_002635"
     CDS             complement(167..625)
                     /locus_tag="LR726_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL6217668.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(638..1969)
                     /locus_tag="LR726_002636"
     CDS             complement(638..1969)
                     /locus_tag="LR726_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL6217669.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1980..3422)
                     /locus_tag="LR726_002637"
     CDS             complement(1980..3422)
                     /locus_tag="LR726_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL6217670.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3409..4173)
                     /locus_tag="LR726_002638"
     CDS             complement(3409..4173)
                     /locus_tag="LR726_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL6217671.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4277..5152)
                     /locus_tag="LR726_002639"
     CDS             complement(4277..5152)
                     /locus_tag="LR726_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL6217672.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5407..5491)
                     /locus_tag="LR726_002640"
     tRNA            complement(5407..5491)
                     /locus_tag="LR726_002640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5455..5457),aa:Leu,seq:caa)
     gene            complement(5509..5580)
                     /locus_tag="LR726_002641"
     tRNA            complement(5509..5580)
                     /locus_tag="LR726_002641"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5546..5548),aa:Gln,seq:ttg)
     gene            complement(5590..5662)
                     /locus_tag="LR726_002642"
     tRNA            complement(5590..5662)
                     /locus_tag="LR726_002642"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5627..5629),aa:His,seq:gtg)
     gene            complement(5724..5808)
                     /locus_tag="LR726_002643"
     tRNA            complement(5724..5808)
                     /locus_tag="LR726_002643"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5772..5774),aa:Leu,seq:caa)
     gene            complement(5829..5899)
                     /locus_tag="LR726_002644"
     tRNA            complement(5829..5899)
                     /locus_tag="LR726_002644"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5865..5867),aa:Cys,seq:gca)
BASE COUNT         1824 a         1260 c          903 g         1954 t
ORIGIN      
        1 tttaagaatc ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt
       61 aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt
      121 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
      181 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
      241 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
      301 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
      361 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
      421 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
      481 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
      541 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
      601 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
      661 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
      721 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
      781 ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
      841 gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa
      901 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
      961 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
     1021 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
     1081 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
     1141 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
     1201 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat
     1261 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
     1321 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
     1381 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
     1441 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
     1501 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
     1561 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
     1621 cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
     1681 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag
     1741 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca
     1801 taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta
     1861 tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat
     1921 acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc
     1981 tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata
     2041 atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc
     2101 tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag
     2161 acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca
     2221 taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct
     2281 gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta
     2341 acgtaataca aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg
     2401 attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata
     2461 ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac
     2521 ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa
     2581 gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct
     2641 ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat
     2701 aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact
     2761 tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg
     2821 tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct
     2881 cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata
     2941 aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt
     3001 tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc
     3061 catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc
     3121 ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt
     3181 cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag
     3241 tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct
     3301 gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt
     3361 ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc
     3421 atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat
     3481 taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg
     3541 atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag
     3601 taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc
     3661 tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc
     3721 aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt
     3781 ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg
     3841 ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa
     3901 tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt
     3961 tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat
     4021 ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga
     4081 tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc
     4141 aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa
     4201 ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga
     4261 tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat
     4321 tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac
     4381 aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt
     4441 cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag
     4501 tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg
     4561 tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa
     4621 tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat
     4681 tgattcgctc gaccacttct actgctatat catattcatt tggataaagg aatctcattt
     4741 ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa
     4801 tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta
     4861 tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct
     4921 cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa
     4981 catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt
     5041 ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg
     5101 ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt
     5161 ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc
     5221 gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac acataataaa
     5281 agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga
     5341 ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga
     5401 taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt
     5461 ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg
     5521 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     5581 agaataaagg gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg
     5641 ttaaccactt cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac
     5701 taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg
     5761 ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat
     5821 tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt
     5881 accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata
     5941 g
//
LOCUS       DAFGFZ010000142         5902 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000142 DAFGFZ010000000
VERSION     DAFGFZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.144"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            199..1002
                     /gene="lnu(B)"
                     /locus_tag="LR726_002645"
     CDS             199..1002
                     /gene="lnu(B)"
                     /locus_tag="LR726_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HBL6217673.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            1690..2511
                     /locus_tag="LR726_002646"
                     /pseudo
     CDS             1690..2511
                     /locus_tag="LR726_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            2654..2878
                     /locus_tag="LR726_002647"
     CDS             2654..2878
                     /locus_tag="LR726_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217674.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            2893..3762
                     /locus_tag="LR726_002648"
     CDS             2893..3762
                     /locus_tag="LR726_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL6217675.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3743..4477
                     /locus_tag="LR726_002649"
     CDS             3743..4477
                     /locus_tag="LR726_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL6217676.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4510..5418
                     /locus_tag="LR726_002650"
     CDS             4510..5418
                     /locus_tag="LR726_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBL6217677.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5415..5663
                     /locus_tag="LR726_002651"
     CDS             5415..5663
                     /locus_tag="LR726_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBL6217678.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(5666..>5902)
                     /locus_tag="LR726_002652"
     CDS             complement(5666..>5902)
                     /locus_tag="LR726_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL6217679.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         2020 a          875 c         1244 g         1763 t
ORIGIN      
        1 tgagccattg aactatattg atattttttc acgtatccaa attgaaaaaa tgattttgga
       61 atattgtcct acactattgt ttgtggagca tgatgatgct ttttgcaata acatttgtac
      121 gaaaaatatt aatttaggtt tgtagagatt ttgagttgcc acaataacta aaagatattt
      181 acatgaaagg gtgaagaaat gttaaaacaa aaagaattaa ttgcaaacgt taagaatctt
      241 actgagtcag atgaacgaat tacagcttgt atgatgtatg gatcgtttac caaaggagaa
      301 ggtgaccaat actctgatat agagttctat atatttttga aagatagtat aacctcgaac
      361 tttgattcat ccaactggtt gtttgacgta gctccgtact tgatgcttta taaaaatgag
      421 tacggaacag aggtagttat ttttgataat cttatacgtg gggaatttca tttcctttct
      481 gaaaaagata tgaacataat cccctcgttt aaagattcag gttatattcc tgatacgaag
      541 gctatgctta tttacgatga aacagggcaa ttagaaaatt atttatcaga gataagtggt
      601 gcaagaccaa atagacttac tgaagaaaat gctaattttt tgttgtgtaa tttctctaat
      661 ctatggttga tgggaatcaa cgttctaaaa agaggagaat atgctcgttc attagaactc
      721 ttatcacaac ttcaaaaaaa tacactacaa cttatacgta tggcagaaaa aaatgctgat
      781 aattggctaa acatgagtaa aaaccttgaa aaagaaatta gccttgaaaa ttataaaaaa
      841 tttgcaaaga ccactgctcg attagataag gtagaattat ttgaagccta taaaaattct
      901 ttgctattag ttatggattt gcaaagtcac cttattgaac aatacaactt aaaagttaca
      961 catgacattt tagaaagatt gttgaattac attagtgaat aggagagtaa cgttattcac
     1021 agtattgggt actgaaaatt aacagcatac ctcagttttt caaaaaacag cgaagcctat
     1081 ggatatgttc ccgtctaccg atagtgcaaa aagtgtcgtg gatatgtttc cgagaacgga
     1141 catacacaaa tgacattcat tctgtcctct caatccatgt ggaagcagta tgtctgttaa
     1201 caagaaaata attattgttc gtcctgacga gttgttagac tcgaaaagga taaacatcat
     1261 ttttagaacc ggtagccgat ttttggctag cggttttttg ggctctttgg caaatagtgg
     1321 tgattgaatc tcgcgctatc ctatggaaaa ccgccttgtg tgaggggctg cgagaccgct
     1381 tcgatcctag aattgctgaa gcacgaatct agcgtcactc acatttcatc ttttgacgta
     1441 gctgagacaa ggtgtttttc ttcgatccgc tagtggttaa tctctttttg gtgcaaatat
     1501 ggtttagaat ttctttgcat ggcttaaggg agtggagaat cggatattag taggatttac
     1561 atccacagat aaaaataggc ttgattttaa cagcgaaact aaaataccct acttaaatga
     1621 agtgatagat tagatgttgg gttgttagga gatgcttaaa aatcctatgc aatcaatagt
     1681 ttttagtgtt tgttgacttg atccctaaat cgcctgagta ctaaacgttg agtgtgtctg
     1741 cttactgtca ctagccagta agtgagactg tagttaatct ctcattttcc ccagtattat
     1801 gttgaaaata gccatgaagc gattatttct aaagagttat ttttgcaggc tcaagaagaa
     1861 atttatcgga gaagtaatat ctacacagga gaaggcaaga acaaaagaat ttatagtagc
     1921 aagtacgctt taagtgccat caccttctgt ggatattgtg gcgatattta taggagaacc
     1981 tattggaata ttcatggcag aaaagaattt gtctggcgat gcgtgactag aatcgagcag
     2041 ggtcccgagg tatgtaagaa ccgaaccgta aaagaagatg aactctatgg tgcggtaatg
     2101 accgcaatta ataagctact tgctggtggc aacaatatga taaagacact ggaagaaaat
     2161 attcatgctg tgattggaga aaccacagaa taccaaattt cagagattaa taccctacta
     2221 gatgaaaaac aaaaagaact aatcaagctg gccaataaag gtcaagatta tgaatacctg
     2281 gtagatgaga tcgatgagat gcgagataag cgtcagactc ttttagtaga agatgcctcc
     2341 ctcagtggcg agaacgagcg catcaatgag ttgattgaat ttatccgcaa gaacaaattc
     2401 cgcaccttag aatatgatga taagcttgta aggaagataa tccagaacgt taaggtctat
     2461 gaagaccact tcgtcatagc cttcaaacca ggtatcgaaa tggaaatatg aaaaccagaa
     2521 gtaaatagcc catgactctg aagtagagtt gtgggttttc ttttaattgc tgattataat
     2581 aatattttaa tgactattgc atttatcaac caaatggttt atgttattct tagcataaac
     2641 ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc aaatgaatat
     2701 aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta ataaaaaatt
     2761 acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc taggtgtcaa
     2821 gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta acgaataaag
     2881 gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt atcatctttg
     2941 ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca tacagaaaat
     3001 tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac agcgattaat
     3061 caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc tcccggagcg
     3121 tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca tgttgacttg
     3181 atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca aggaattgtt
     3241 actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta tcgtggagaa
     3301 ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt aaaaaagcag
     3361 gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat atttgaagct
     3421 gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata ttatattgca
     3481 ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg taataatgct
     3541 tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata taaacctgaa
     3601 aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct ttttgaatgc
     3661 tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga gataaataac
     3721 tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata tattttttca
     3781 aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag aatgggagac
     3841 tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag gatgcggcta
     3901 tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag gtgttgatat
     3961 ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga ttgaatatga
     4021 atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa tactaagttc
     4081 gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat ataggatgct
     4141 gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg ctcatggaac
     4201 acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg acaattatta
     4261 ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat atcatagaac
     4321 actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc agattgtgga
     4381 gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa tgcgacgccc
     4441 aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa ataaacgagg
     4501 agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt agcagaacag
     4561 gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa tatacctaaa
     4621 gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc gtttatatct
     4681 aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc ggaggatatg
     4741 gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga tgattacaat
     4801 aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa gggcgataaa
     4861 ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt tccgactgat
     4921 atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg caatgaattt
     4981 tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt atttgctatt
     5041 gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg gaaggtcggc
     5101 atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga aaggtatata
     5161 tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta tgaaaacata
     5221 tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga ggtggcggaa
     5281 aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac cagggacatg
     5341 tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc tgatatagaa
     5401 gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat atcgataaac
     5461 ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga
     5521 cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg
     5581 aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc
     5641 ttttaacccg tggtgctagt taattttaat atcctaaaga atatctcaaa gttcctccaa
     5701 aacgtcgttg tgctcgactt aatagaacac tgtaagccag taatatgctt tcaaatctac
     5761 tctctagccc tttaacagaa cgtgagttga aattttgaca acctaatttt tctaaggaag
     5821 aaaaaacagt ctcaactgtt tttctttgtt ttccaagtaa gctgtggtct agtttttctg
     5881 atttatccat gtttttacgt gg
//
LOCUS       DAFGFZ010000143         5888 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000143 DAFGFZ010000000
VERSION     DAFGFZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.145"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..286
                     /locus_tag="LR726_002653"
     CDS             <1..286
                     /locus_tag="LR726_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217680.1"
                     /translation="DEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKS
                     ELVSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            300..989
                     /locus_tag="LR726_002654"
     CDS             300..989
                     /locus_tag="LR726_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217681.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1114..3465
                     /locus_tag="LR726_002655"
     CDS             1114..3465
                     /locus_tag="LR726_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL6217682.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3545..3940
                     /locus_tag="LR726_002656"
     CDS             3545..3940
                     /locus_tag="LR726_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217683.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4265..4882
                     /locus_tag="LR726_002657"
     CDS             4265..4882
                     /locus_tag="LR726_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217684.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4923..5222
                     /locus_tag="LR726_002658"
     CDS             4923..5222
                     /locus_tag="LR726_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBL6217685.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5256..5423
                     /locus_tag="LR726_002659"
     CDS             5256..5423
                     /locus_tag="LR726_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL6217686.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5468..>5888)
                     /locus_tag="LR726_002660"
     CDS             complement(5468..>5888)
                     /locus_tag="LR726_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217687.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2019 a          813 c         1194 g         1862 t
ORIGIN      
        1 agatgaagaa ttgtataaag aatttggtga tttatccggg gaacagttag aagaaagtat
       61 tccaggaatt agtgactcag ataaaattgt tgtcttgtct gctggtgggc aattaatggg
      121 agatggcaaa tccgaattag ttagttataa acaaaatcac agacagttta ttgatgttaa
      181 tactgattcc aacgctattt cgattaagga tctaaaagag cttttaaata gtaaaagaaa
      241 tgaattaaag cagataaaag aaactaattc agtggaatta gagtaaagga agattcatta
      301 tgaaaaagat ttttggtgtt tctgttgggc tacttatgat gttaacattt ggaaatgcag
      361 tatatgctgc tgagaaagaa gttatttcag atccagagca gtacgcctca cgatactttg
      421 ctgattttga agaagaagat aaagtacttg cattgccaga aggtggtttt ttaaaagggg
      481 aagctaaaat tgtatctatg gataatccag atataattca aggttcatat aattcagaaa
      541 ctgatcctgc ttcagtttca gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa
      601 atgatattga aactactcca attatgcctc gaggagcatt tccacccgat gttggtacta
      661 gcggacacct tcttgtacta gcaggaggag gaagctataa atcacagcca tttagtggtg
      721 cgggatggcg ttttggaggt atgggtgtaa aaccagctga tggtacaggc aactttttac
      781 gttggaaatc ctacattgac gatggaagag taggaaatgt aaatcaagcc aacgcaacta
      841 aatctggttc tattcaagga atggcattgt atgctcgtga aggattcaaa tactttgcac
      901 caaacgggcc tggttggaca atgaatatag gtatttatta cacttataat cctgttgcag
      961 gtacgtatta tgttgttcaa aatatttaat aaaacagcac aaagagattt ctttgtgctg
     1021 ttttattatt tttagttgtt gacaaacact aaaatataaa atataatcaa cacataaacg
     1081 aacgtatgtt cggcaagaaa gcaggggatt tttatggatg agatggtttt aagtactcag
     1141 aaatggctaa acaagaaata cagtaatgtt acagggtttg ataaggttcc agaaaatggg
     1201 cgaacagggt ggcctacaat ctatggactt attgaaggac tacaagtaga acttggtatt
     1261 actaatctag tagctaattt tggacctacc actgaaaaaa tgtacgataa tcaggttact
     1321 cctaaatggg gcaaaaatct acctaaaaat attgtatttc ttattcaagg tgccttttgg
     1381 tgtaagggga ttaatccagg tggcttcgat ggggtatata ctccatattt agatactgct
     1441 gtaaaagagt tgcagactga tgcagggttt aatggaagcg ctgtaactgg agttttggat
     1501 gcgaaatggg ccaaagcgtt atttgatatg agtgcttttg ttcttgttcc cggcggagat
     1561 agtaaaatta gaaaaatgca gcaatggtta aatgtaaatt atttagaata cacgggaata
     1621 atgccttgtg atggtattta tcaaagaaat tcaaatcaag cattaatttt tgcattacaa
     1681 gctgaactag gatattctcc cagtgaagca acagggtctt atggtaatgg gacaactagt
     1741 aaaacttatc ctgtgtctga aggtaactcg ggaaactatg taagaataat tcaatatgga
     1801 ttatatgtta atggttattt tgaagacggt acattcgatg gtattttcac taaatatatg
     1861 ggtttagaag tactggcatt ccgaaaattt atggtattgc ctgaatatac tgaaatagca
     1921 gatgttgttg taataaaagg actattgtct agcgctggag atattagaag atctgcagat
     1981 ggcgcagata cttctacgca attaactaga tctcaaatcc aaacattggt tgataatgat
     2041 ataaaaatag ttggtagata tcttactggt actgttggaa ttggaaaaga tgaacgaaat
     2101 aagtatctaa ctacagagga attgaataat attttttcta aaaatttatc tgtgtttcct
     2161 atctatcaag atggaggtgc agcattagct tattttactt atgatcgtgg cttatcagat
     2221 ggtaagaagg ctatagatgc agcaaaaaat ctaaatatac cattaacaac agttatttat
     2281 tttgctgttg atctagacat gttaggggaa gaaattcttg catatgctgg agaatatttt
     2341 aagggggtat ctgctgtaat gtcatatagc gggtatcaaa cgggtgttta cggaacaaga
     2401 aatgtttgtt ctcaaataat caataacggg tatgcttcgt ttgcatttgt ttcagatatg
     2461 tcgactggtt atagtgggaa tttaggtttt cctatgccta gacaatggtc ttttgatcaa
     2521 tttgttgaat ttaccatagg aagtggcaac ggagctgttg gtatcgattt aatcgctaca
     2581 tcgggtagag atagcggatt taatgagtta tcaaatgata ctaataatga taattacatt
     2641 gccaaataca atcagaaggt tataaatcag atggtacgtg cgtatcaata tctatcacaa
     2701 gctggtcttg ataatccttg gaaccctcat ttgacatttt atagatatgt aaattattct
     2761 ggtttgtcat gggacatcat ttcttctcct gtaacagagc atgatagaaa aatttatgat
     2821 gaatatagga ataaattaac taatagcgag ggcctgtata attattttat agatcctaat
     2881 actggttcaa taataggttt gcctcactta attgtgacgt tacaatcaca gatgtttcta
     2941 gccgacacaa tcaatgatag tgtttctgac tttgcaggtt ggttaggaga tttaatgact
     3001 tgctggggtg aagttaagaa attaggaata caaatggagc aaggtgtatt tgcattagta
     3061 ggtacggctg caggtggaac atttagtcta gaagatttat accaagatgt tgacgcggta
     3121 aatgtttata ccttagcaaa aagaaattct gattcaacag ctttatcggc tttaaaaacg
     3181 tactattgtg ataagggtgc tattgataga tttaatcaat ttgttaagaa cagatttggt
     3241 tcttcagata aaatcgcttc agtaacaaat agtttattag aaaatgacga aacagttgat
     3301 atcttaggtg tagggataat ttcatttttc attgataaaa tagttaataa ttcacttggg
     3361 acagatttta cttatcttga ttttattgga gatgagggga aagttgcaaa tggattttct
     3421 gaaaaaatta ttcaatttca agaaaataga agtaaactta attaagaatc gtttactatt
     3481 taaacagtgt ccttttcctt tttaaatata tacgataaaa taagtatatg aattgtaaaa
     3541 ggacatgatg attatggaag aaatagaatg gaataatctg actcaaaata ataaaaataa
     3601 ttctggatgt ggaattatct ttatgtttgt tatagttttg ggaatagtag tacttttaat
     3661 aactgtcaag atagtagatt actttgcgaa tactcctgaa gaacaaatac agagaaaact
     3721 tcctgagttt gaaagagtac caccttatga tccaagagat aattcatttc aagtaaaaaa
     3781 attaaaagag tctccaaatg aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa
     3841 agaaaaaaag aaagaataca attatattgt aaaggcaaaa gtatcaaaaa gatttatttt
     3901 taaccctaaa ttagaaatat atagtgttgt aatagaataa tagtgttaaa aaaattgtaa
     3961 aagttaaaga agtcaaattc tgatgtgaaa aataaaatat accatggaag acataaaaga
     4021 aaaactggat aactagttta tttaatggca gaccgttaag gcctgccatt tttatgtatt
     4081 ttagcggaat aaatataatc atgcgggagt gtaaacttac tgtttcaaaa agtatacttt
     4141 ttgacatgcc ttgattttct ttgtttatgt atggcataat aaccacataa tacaatttga
     4201 aacgtttctg atttaatctg attgttttga aacaatttta gtgaaaaaag ggtagataaa
     4261 aacgatgaga aaattttttt atatccgggt cagtagttat gatcaatcga tcgaacgaca
     4321 actgatttcc gcgaaggaat taggaatccc tgaagaatat atatttgtag aaaaagccag
     4381 cggcaaagat tttaaacgcc ctgaatatca attaatgaaa cggatgtttc gaaaaggaga
     4441 tttactctat ctacaatcat tagatagact aggcagaaat aaacaaatga ttttagaaga
     4501 atggcgagaa ttaactaagg aaaagcagat tgatatttct gtgttagaca tgcctctgtt
     4561 agatactgct agttacaagc aactagatgg gttagagacg cttatctctg acttaattct
     4621 gcaattactc agctatatgg ccgaagacga gcggaaacga attaatagcc ggcaaagaga
     4681 aggcgtagaa gctgcgaaac aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc
     4741 tcctgaattt cctgcgatct attctgactg gaaagcagga aagataactg cagtctatgc
     4801 tatggatcaa ttgggcatga aacgaaatac cttttatcga cgggtgaaag aatatgaaca
     4861 taccttgcac cacttttctt gatagatata acagctatta gacgggaagg gagagtttga
     4921 aaatggactt aacgaatcaa agggtcgttt ttactggaac gctgcagcaa atgacacgaa
     4981 cccaagccat cggtttggtt cactctttaa acggccattg tcaatcaagt gttacttcaa
     5041 aaacaaattt cctggtttgt ggtcagtatt ctaagtcgtt gtttgatgat tctctctata
     5101 caaaaaagga acaaaccgcg cgggacttga ttgcgttagg tcatgagatt accattttat
     5161 cagaagtcga attttatgcg ttaattagtc aacagttgaa agaatggcaa cggtatttat
     5221 aatcgttagt aaaatttaca aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt
     5281 ggggcattga aataactagt tttaatcgaa atattgcttt tcacgtggag tgtgactgtg
     5341 gggaattagc aaaaagtgtt tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc
     5401 gatacggggt acatggaaca tagaaacgaa atagtgcggt agaaaagcga atgaatgtag
     5461 tatatttcta caaatcattc gctttcttta ttttataaat agttggtcga gagatcccgt
     5521 tttcttttgc aatctctgca actggaatgc cttgctttaa catgtccacc acggttttaa
     5581 aaactaagcg tttttgcgga tctgttgagt taatagaata ctcgattggt cgtcccttat
     5641 atcggccggc tttttgagcc aataatactc cttgccgctg acgttcacga attttctcac
     5701 gctcgttttg tgcaatataa cttagcagtg ataaaaacat atcgaacata gcggtatcca
     5761 ggagttcatt tccagtatta aaatctaaaa actgtgcatc taatattttt aacgctactt
     5821 ttttctgttt cataaaagca actgtatttt ttatatcttc ataatctctt cctaaacgat
     5881 ctagtgat
//
LOCUS       DAFGFZ010000144         5820 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000144 DAFGFZ010000000
VERSION     DAFGFZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.146"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..172)
                     /locus_tag="LR726_002661"
     CDS             complement(<1..172)
                     /locus_tag="LR726_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217688.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVT"
     gene            complement(166..567)
                     /locus_tag="LR726_002662"
     CDS             complement(166..567)
                     /locus_tag="LR726_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6217689.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(754..2562)
                     /gene="uidA"
                     /locus_tag="LR726_002663"
     CDS             complement(754..2562)
                     /gene="uidA"
                     /locus_tag="LR726_002663"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBL6217690.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2585..4351)
                     /locus_tag="LR726_002664"
     CDS             complement(2585..4351)
                     /locus_tag="LR726_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBL6217691.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4364..5158)
                     /locus_tag="LR726_002665"
     CDS             complement(4364..5158)
                     /locus_tag="LR726_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL6217692.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5168..>5820)
                     /locus_tag="LR726_002666"
     CDS             complement(5168..>5820)
                     /locus_tag="LR726_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217693.1"
                     /translation="IIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIVLMNFINPILV
                     LIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLG
                     VFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYD
                     SLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         1870 a         1149 c          712 g         2089 t
ORIGIN      
        1 aggtaacgaa ttgacctacc tcgttaaatc catttgtcaa acataactta gccatataag
       61 aatgataatg tttgagtatc atcttgatcg aaattggatc tccttctaga gcagataaga
      121 taatggatag tggaactaat aggtactgcc gctttttaga tttatctacc ataggtcata
      181 ccccattttc aattgttcaa ctgccttctt tcgttgatac cagataccac ttgtgctcat
      241 attgagaatt ttaccgattt cagcatcatt aaaacctgca aaataataaa gtaggataat
      301 ctttcttctt atctccgata actgatcaat tgctgctgat aacttttgat ctgaaattaa
      361 tatttccatt ccaaataaga aatagacatc ttgttcatca aaatgagttt gggaatacct
      421 gccttcattt gaatctgcca atggttcctc attatccttt atgttagcgt ttcgttttcg
      481 aatagactta gcttcgtttc gtaatacttt tttacagtag gcatcaaatt ggtgttgtat
      541 agatatgtca ttgtaatgtt gttgcatgat aatatgctcc tttttccccc tttccactaa
      601 tacaacaatc agaaagcccc tttttttcga aaaaactaga aaaaaaacat aaaaatattt
      661 atcattcttt aaacagaaaa tctttctata aaaaacaaaa aaaaacgatt aggctaggct
      721 aatcgttttt tgaacattct taaaaaatcc aaattatttt ttataactga aattgggtat
      781 tgatgaccat cgtttctgta gatagtaagc aatcatttta ggttttctat ctctagtaaa
      841 gattcctttc ttatttcctt gcactcttaa aatcccttga cttgttgcaa aatctgcaaa
      901 attccacact tgttcaccaa caaaattttc acatgtatct attacttcgt gatttgcttc
      961 gtaataagcc acttgatact cttctgtaaa cattgttggt actgtatcat gaaatccagc
     1021 tactgtatct gctccatatt ctgtaaacat tattggcgta tcaggacatc tttccatcca
     1081 tccatctagc tcttttttca acagatattt tgcatgtgcc agatcccctc ccgaaacata
     1141 ccaaccataa taacgattta aacataaaac atcgacaata tctcctacct tacaaacatc
     1201 tggcgttgat tttagataag taacgattgt tacaggtctt ttctgcggat caagttcttt
     1261 agttaattca actaaaggtt caaaatattc tttagcacct tcggaatccg aatctggttc
     1321 atttgcaata gaccacatta caacacttgg gtggttcttg tcgcgttcta tcaattcctt
     1381 gatcaccgtt ttatgagaat tttctgttcg tatctctttc cacgtattat gtttttccac
     1441 attatcatct aataaagtgg ccatgaaatt taaatgtaac cctactgcag gtacttcatc
     1501 aataactaca ataccttctc gatctgctaa tcgcataatt tcttctgagt aaggataatg
     1561 tgatgttcta aaagaattag cacctatcca tttcattaaa ttaaaatctt taatatttac
     1621 agcctcattc aaaccacgtc cattaacata cgaatcctca tgcttaccaa atcctttaaa
     1681 ataaaaaggt ttattattaa tcaaaaattt gccattggta acctgaacac tgcgaatacc
     1741 aaattcttct gtataggtat cgatcacttt attatcatca attaaactaa ttttcatact
     1801 atataaataa gcgtctagag gttcccaaaa aattggattc ttaagctgaa tcattccttt
     1861 ctttccttca gcttcagcta caagttgagc ctcttcgtca tatatcgcta ttaagatttt
     1921 tccggtccat tctgtatcga tattgaattc tacacttgca tccaaatcag ttattcttgt
     1981 attaatagta atatcactta tatagtcttt cggagttgta tataacttaa caggtctatg
     2041 aatacctgca taattgaaga aatcaaaatt aactaagttt tcctttttta tatccccatt
     2101 ttcctgtact gtttccttca taatccccac aggtaaagta ctatcgtcaa taatattgtt
     2161 tacagcaact gtaattcgat tattcccgtc ttttacaaac cgattaattt cagactcaaa
     2221 tggtgtaaac ccacctttgt gttgagtaat aaactttcca ttcaaaaaaa cttttgcttc
     2281 atgtgttgca gatccaaaac gaagaactaa tctttcttct aataatcttt taggaagagt
     2341 cacttctttt tcataccaca cccatccaac atgatttcta atgttctctg tttcaaataa
     2401 atcattataa gaagagggaa ctgaaatcgg ataactctct ttttcattta ttggttctaa
     2461 agcaagttct tcctgtaagt catcatttgt atctaattta aatctccaga gaccgcttaa
     2521 gtcaattact tgcctcgtct ctgtcataat gggatacaac atataaacca ctcctatcta
     2581 aatattaaat aaatgtatcc tttaaaccac aataagcatc tattggactt actcccttaa
     2641 attcagaaag acccattagt ttgtccacca atatttctaa agtttcttgt tttccatcat
     2701 aagtattaat ataagtcttt acttgaggta catcagccaa atgaaacgga cactgtacag
     2761 atacaaaaat tgttggaatt tcatgaatgt aaaacggtat atccggagtt cctttacttg
     2821 ctggccagac aattcgttga actgtgcctg gattaacatt agctatatta atgatcaagt
     2881 catatttttt agttaaattt ttaattggtc tcttagcagc atatattcca gctatatttt
     2941 taattttttc ttcttgcgat aatcctttca attcttcttc aggtgaaatg tatttttcaa
     3001 cttcaaatcc ttttgctgac aaaatctgtt tcaataaatc aatggcttga ggtctatttc
     3061 cagcaactac ttcaccaaac ccacctttta ccccatgtac atctactagt aaaatccttt
     3121 tatatttttc tggtacaata ggccaaatat cttgtttatc tttaactaat gtaattgctt
     3181 ggtcagccac ctctttaaaa attgatttat tttcaattaa accaattttt tccagagctt
     3241 cttgcttagg taataaaatt tgttcttttg cttttttgtg taatcccaat ttagctttta
     3301 accctaaaat tctttttaag gcatctaata accgatcatc agtgataatt cctttacgat
     3361 agccttccat catataatta aagtcttcat ctggatcatt aaaaaataga aatagatcac
     3421 atccagctgc aatagaagtg ggcaataatt ctgatcgttt cattgcccca gtcattccta
     3481 acatatgact agcatcagta actactaatc cattaaatcc gagttttcct ctaagtaagt
     3541 cagtgattaa ttcttttgat aaagttgcag gaagtaaatc ttcatctttc agatcaggat
     3601 taaaatgttt tgaataagct ggcatatgga tatgacctgc cataattgaa ggtaatccag
     3661 cttctattaa gcctgagtat acttttccaa aagaattatc ccattcttcc gtacctaaac
     3721 tattaatact aaaagataga tgctgatctc gttcgtcaat accgtcacca gggaaatgtt
     3781 tagctgcagg caaaattcca ctctcctgaa tacctttcat ataagctaaa ctaagttcta
     3841 aaactttatc agggttttgt ccaaaagatc gactagaaat gataggattt ctccaatttc
     3901 tattaatatc tacaatagga gcaaaactcc agttacatcc aatagccgat gcttcaatcc
     3961 cagaaactct ccctaattca taagcatatt tcgcatcatt tgttgcacca atttttactt
     4021 ctacaccaac ttcagtacca tctgtacaag ctccgttacc acctgcttcc gtattagcag
     4081 caattaatag aggaatttta ctttctgttt gtaagatata gttttgatca tatacctcat
     4141 cagcagttcc tggattatat cgtacagctc caatatggta ctgattgacc atattactca
     4201 agtaattttt atcacgacta gatcccatat taacaaacaa ttgacctatt ttttcttcca
     4261 aagtcataga atcaatcgtc tcttcaatcc aagaaatagc atcttcatct aaattatatg
     4321 gtttttttcg taaatctact tttatagtca ttttctttct cctctactta ttagtaagat
     4381 ttttccaaaa agtatctata tatttttgtt caatatctac ataatctccc gataaacatt
     4441 taaataaaac atcagttctt aagttttccc atgattcttt ttctttattg accaaaatag
     4501 gataaaagaa aactttattt ttatttgcag cttctagatc accaggagaa tcaccaatca
     4561 ttaatatctt atccaaatca caaccatttt caattacact tcgaataata tcttcttttt
     4621 ttcctttatc ttgacaacac agttcatcaa ctaatgataa taattcatgt ctttcccatt
     4681 cttcttgaac agcttcctta ttagctgagc ttacaataat tatcttccca tattttttca
     4741 gagcttttat agtatctcta gcacttaaaa agggtttatc taatcctttt gcttgactaa
     4801 tctgttgatt gacttcttcc gaccatttta aagcttttaa taaatcctct gactgagaat
     4861 attctatttc cctcttcaaa gatggattag acaattcttt tgttagagat acccagtgct
     4921 ttaatcgatt aatcgatgtt acatcatttt tttctaatgt tttaactaaa cctaaaaatc
     4981 tgtttattcc acgagtctct gaatataaat taatattatt ccaatcctgt aggaattttt
     5041 ctctatgatg aacatcaaaa atctctgcag caattggtcc gaaaaagaga ttgtgtttat
     5101 aagtcatcgt atccataaca cagccatctg aatcaataca aaatatccaa ttattcatat
     5161 ttctctccta tttatgtttt tgttttaaaa gctcgcaatt ttctaagact ttatctttag
     5221 ataatggata aaggaaaaat aaaattaatc ctacaatcag aaaacaaatt gatggaatta
     5281 atgttgaagc gttatataac ttttctaaaa catctcccgt ttgtacattt gctaaagagt
     5341 catagcctat aatagaaaga gtccaacctc cgacgcctcc agctagagct tgacctattt
     5401 ttctagcaaa agaatatacc gcatatacgg tcccatcttc tctttgatca gactttactt
     5461 cttgatcatc aataacatca gtaatatttg cccaaataac agtattaaac acacccaaac
     5521 ccatatagcc aaccgaacta agaattaaga atacatacat attactagct tttatcaaaa
     5581 ataagatcaa atatactatt gctgaaaaga acattcctac tgatgccagt tctttttttc
     5641 catatttttt acttaaaaac aaagttaaag gcgaaacaac tattaaaacc aaaataggat
     5701 tgataaaatt cattaaaact attccctgtg catcattata gtaatcagga aatacataat
     5761 tattcatact cataattaat aattgagcaa ggagtaagaa aatagccgca ccaattatag
//
LOCUS       DAFGFZ010000145         5764 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000145 DAFGFZ010000000
VERSION     DAFGFZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.147"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            153..1049
                     /locus_tag="LR726_002667"
     CDS             153..1049
                     /locus_tag="LR726_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL6217694.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1199..2140)
                     /locus_tag="LR726_002668"
     CDS             complement(1199..2140)
                     /locus_tag="LR726_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL6217695.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2266..3387
                     /locus_tag="LR726_002669"
     CDS             2266..3387
                     /locus_tag="LR726_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL6217696.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3645..5147
                     /gene="eat(A)"
                     /locus_tag="LR726_002670"
     CDS             3645..5147
                     /gene="eat(A)"
                     /locus_tag="LR726_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL6217697.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5175..5741
                     /locus_tag="LR726_002671"
     CDS             5175..5741
                     /locus_tag="LR726_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL6217698.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1890 a         1018 c         1215 g         1641 t
ORIGIN      
        1 ttaggcgaat tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat
       61 tttcatacaa atataggaaa cgttttactg gctttttact atttaaacag gctatcatag
      121 gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg
      181 caaaaaataa atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa
      241 aagatttact acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc
      301 tgatcgtttt tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta
      361 ccaatgcaaa aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc
      421 tgaagcaggc acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag
      481 caaaagaact tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt
      541 ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag
      601 cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc
      661 cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag
      721 gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga
      781 catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt
      841 atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac
      901 cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg
      961 cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat
     1021 atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg
     1081 aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta
     1141 ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt
     1201 attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg
     1261 gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt
     1321 cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt
     1381 tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc
     1441 cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg
     1501 ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg
     1561 ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat
     1621 ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt
     1681 ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta
     1741 gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca
     1801 gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa
     1861 tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca
     1921 agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga
     1981 ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag
     2041 caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc
     2101 ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc
     2161 aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa
     2221 aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg
     2281 gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat
     2341 ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat
     2401 actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat
     2461 ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc
     2521 gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa
     2581 actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa
     2641 gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga
     2701 ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct
     2761 actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc
     2821 gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga
     2881 caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct
     2941 cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca
     3001 ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta
     3061 gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga
     3121 gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt
     3181 gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa
     3241 ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa
     3301 gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc
     3361 agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat
     3421 ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag
     3481 ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc
     3541 ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc
     3601 agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa
     3661 taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc
     3721 taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa
     3781 ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag
     3841 aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa
     3901 atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag
     3961 atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc
     4021 tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc
     4081 atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct
     4141 tcatcgtggt cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa
     4201 tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa
     4261 aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc
     4321 taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa
     4381 agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg
     4441 ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga
     4501 tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata
     4561 gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa
     4621 atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag
     4681 gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca
     4741 aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt
     4801 atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag
     4861 catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg
     4921 agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag
     4981 ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat
     5041 tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct
     5101 tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc
     5161 aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt
     5221 atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag
     5281 atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag
     5341 atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag
     5401 cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa
     5461 tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt
     5521 tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg
     5581 ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata
     5641 atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta
     5701 cattcatggt agataataca gaattggttt cttatgagta attatgtttt tgtgttattt
     5761 aaat
//
LOCUS       DAFGFZ010000146         5706 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000146 DAFGFZ010000000
VERSION     DAFGFZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.148"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(260..1579)
                     /locus_tag="LR726_002672"
     CDS             complement(260..1579)
                     /locus_tag="LR726_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL6217699.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1836..2306
                     /locus_tag="LR726_002673"
     CDS             1836..2306
                     /locus_tag="LR726_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL6217700.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2528..3631
                     /locus_tag="LR726_002674"
     CDS             2528..3631
                     /locus_tag="LR726_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL6217701.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            3852..4811
                     /locus_tag="LR726_002675"
     CDS             3852..4811
                     /locus_tag="LR726_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL6217702.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(4798..>5706)
                     /locus_tag="LR726_002676"
     CDS             complement(4798..>5706)
                     /locus_tag="LR726_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL6217703.1"
                     /translation="KALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVL
                     STYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAI
                     GFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSIS
                     NALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEI
                     EDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDK
                     LKMLDYLFHPSFSQPYNFIFSFVKLS"
BASE COUNT         1890 a          981 c         1110 g         1725 t
ORIGIN      
        1 aatattgttt tattagtctg tacaccaaat gaaatatttg taaaaaagat aggcagcctg
       61 aataattcat agctttaatt ctttggcaca ttttattgaa atatgtatca ctaatatccc
      121 tatcaactga ttttgactaa aaagttactt caaacattca ctgttcaaaa atattgagga
      181 ataaattttg ggtgtaaggc attttgagca tacttctccc tggataactt cccagtaaaa
      241 aaatcgttag attgttggat tagatccatt gactggctcg cctcaggcga gcaaagaccc
      301 tgtagaattc attctgatac acatgataac tagataattt gagatagact cgtctacccg
      361 tttggactaa tttcccagca actttcagaa acttcaatcg aatagaatga atggtcattc
      421 ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt gactaagttg taagctatca
      481 agctgagcat cattcggaca tgattctcca aaaagcgagg actgtctgtt ttgtcaaagt
      541 aaaagccagc tttcgcttct ttaatgaagt tctccattgt gccacgtttg gcatagatag
      601 aaaagatggt atcaggagaa acattttctg atagattcgt cacgataaat tcatgtcgaa
      661 agagtagttc gcccgcttca cgtgttgagc gtatacacac gcgacgactt tgtgaccagg
      721 attgtgcttg ataagtggtc gagaagtact gaacttctcg ttcttcccac ttttgattat
      781 cgccataaag tactgatttc tcagctattt gacctagtct acgattattc ttcagtcgaa
      841 taacataatg actctttttt gattcacacg aatcatacac accaggtgta gcgaacccgc
      901 tgtctccacg aaccaagatg tcagtgtttg gtagagagtg attatagtgc tctaataaag
      961 gtgtgagaaa ctcctttacg ccttttgatg tatattgatt tcctgaacgt agttcagctt
     1021 ttaagaagtc accagtcaat ccgtcaaaag ctaccaatgg atggtaacca taggtttggt
     1081 agtgtgtatt ataattcgtt tgttcttggt gaccaaatgt atctgaatgg gtcgaatcta
     1141 aatcaatgat taattccgta tcattacgga tgaggcgtgc tttatcgata agttcttggt
     1201 tcaaagcttg aagttcatgg atattctcct cagatagtcg atctaaaaat cgggaaagtg
     1261 aagattgaga agcgagttct ttcctatcta aaacagcttt aaacacagga tcttgtctca
     1321 ggagattagc tgcagaatca gctgagtaac cagcaattaa ttgcataatg aactgctcaa
     1381 gtatggataa gttgtcatga gtaaaatacg ctcgttcgtc ttcaatgtga atgtgttgct
     1441 tagccagttc agaaaaacca aagatgttca tcagctcttt cactaggacg agacccgaat
     1501 cactcgataa ttgaccacct gtatgagaaa tagtgatatt tgaattgaat tttacttggt
     1561 ttttgtgtaa gctagtcatg aaaagaactc ctttcttttg gttggtttag tcactttaac
     1621 catagcagaa aggggttctt ttttcatcac ttaacgggtg aagatgaaaa agtaattgta
     1681 agctgccgtg aggcagtttc acatggtttt aattaaaagt atgaattatt caggtagaat
     1741 ctctgaagaa taaatcacgt gatcgtgacg ataaaataca cttacgtcag gtgataaaaa
     1801 atacataagt agaaaactaa gaggaggaac agaaaatgaa agaagttcaa ttgatcagaa
     1861 aaagcgaact atcagaaggt ggttgcaatg catgcggcgt agtagaagca acaagctata
     1921 cattaaagct tggtgccaac aaagcgataa tctctgagtt gacagtaggc ggtcttgtgg
     1981 acagtctggc gcttgctgaa ggttttattg gagaagatat ttatgagatg ttttcagaaa
     2041 tccgtcagtt aaaaaaaggg gaaaactgca tagaggttca tcatgagagc ccgaatgtga
     2101 gattcaaacg tggtgataat gagatgatct tcaataatca tgtgtccgat cacaccgagc
     2161 tatatggaat cgtgaatcaa atactaactg agctttttgg attgggacct tatgcgttca
     2221 aggaagaaaa cggtaatcca aaattaaacg aagaatggca agaaacgatt gaaatacaaa
     2281 gaaacaaccc gcacttattt caataggcat ccacagtatt tcgtaaaagg gactttgaca
     2341 cgacttttgt caaaatccca tcgtttttta tctatttgtg ttctaaacat ttctttcaga
     2401 gtagtaacaa ctggaatgac tgaaatgtaa acagattatt tgcctgtatc aatacggaag
     2461 agtaatataa agtgtgtaaa cgctatcata ttattcattc aattttttat agagggaagg
     2521 cattgaaatg gaaaaaacgt atcaagcagg tacaaatgaa ggaatagtgg attttatcaa
     2581 tatggaagat ttagaaatag ccgcttcaca agtgattcca gctggtggtt atggttacat
     2641 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatca
     2701 gctgattatt cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga
     2761 caaagaaacg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gcttagctca
     2821 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
     2881 aagttcttac gcttcttgta cattagaaga gataagagaa gcaggcgggg agaaagcacc
     2941 gcaatggttt caattttata tgagcaaaga tgatgggatc aatttggata ttcttgaagt
     3001 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
     3061 ccgtgaaaca gaccgcagaa atgggtttac cttcccactg ccaatgccga tcgttcaggc
     3121 ttatcaatca ggagtaggcc aaacgatgga tgctgtgtac aaatcttcca aacagaagct
     3181 tagtccaaaa gatgtagaat ttattgctgc tcattctgat ttgccagtct atgtgaaagg
     3241 tgtccagtca gaagaagatg tctatcgttc attagaatct ggtgctggcg gaatctgggt
     3301 atctaatcat ggtggccgtc aactagatgg tggtccagca gcgtttgatt cgttgcaata
     3361 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgcgg
     3421 acagcatgtt ttcaaagcaa tcgcatcagg tgcagatctt gttgctattg gcagacccgt
     3481 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
     3541 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
     3601 aataaaattg cgtgagaatc gttttattta agaaacaaat gacgaaaaaa ataggagtgc
     3661 ggcagatgat gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg
     3721 aaacgaaaaa ataaaattga aatatttttt catacttcgc cgattgtaaa attaagctag
     3781 acaactaaaa aaagtcattt gtgctacact caaaatagtt ccgacgaaag aattataagg
     3841 agtgagtaca aatgacctat aaccatctta caccaactga actcgtaatg atagaagctt
     3901 atttcaatca atcacaacct gtttcaaaag tagccaatct gttacaacgt tctcgacaaa
     3961 ctatttataa ggtctatcgt tttcttaaat ctggtggaag cgcaatgagc tactataaac
     4021 aatataagaa aaacaaacgt aactgcggaa gacatccgat tgttttacct gatgagcaac
     4081 aggaatacat tcaaaaaaag gttgttcaag ggtgggctcc tgatgtcctt attggtcgag
     4141 cagagtttcc tattgcttgt tcggtacgca cactgtatcg aatgtttaaa gataaaacct
     4201 ttgacactca cgatttacca atgaagggaa aaagaaaacc aaacggtcat aaagaaaaaa
     4261 gaggaaaaca agggtttcgc cggtcaatcc gagacagaaa gacagactat acccagtttg
     4321 atcatgaatt tggtcacctg gaaggtgaca ctatcgtggg tggaaaacat aaaagcgcgg
     4381 tcatcacatt agttgaacgg ctatctaaag tcatcattac cttgaaaccc gaaggcagac
     4441 aggctaaaga cattgaaaat aggctgaatc aatggtttcg ttgtgtccca aaaaacttat
     4501 ttaaatccat cacatttgat tgtggaaaag aattctcaaa ttggaaaaag attagtaata
     4561 ccaacgatat ttcaatttat tttgcggatc caggaacacc ttctcaacga ggattgaatg
     4621 aacattcaaa tggcttatta cgtaaagatg gtttgtataa agctatggat tttaatggtg
     4681 tatctgaaac ctttatccaa tcggtggcat cgaaacgaaa tcatattcct agaaaatcat
     4741 tacactatcg gacaccaatg gaagtatttt tgagttacgt cacaaaagat gatttgtcta
     4801 acttaatttg acaaacgaaa aaataaaatt atagggttgg ctaaaactag gatgaaataa
     4861 ataatctaac attttcaatt tgtctaatgt aatttgttct tctatagcaa gagaaaacat
     4921 tgataatagt ccagataaat cttcttgact acaaagttgt acacctacta ttctctttgt
     4981 atttttttca aagctaattc ttaattttac acgactattt tttctcaaaa aagcaggtct
     5041 aatgaaatct tctatttcta tataatctat agactcactt aataatcgtg ctgtttttaa
     5101 attcaaacct gctgcgtaaa aattgtaatt aaatatagaa aagccatttg taaattggga
     5161 accagaaggt gtaaaatctt gattgttaat atgttgagtt gcaatataag ctgttcttag
     5221 agcattagaa atagaaaaat caacactact tctattaatg gacgtaaaaa aagttgtagc
     5281 acaatcccca atagcatata tatcttttat atttgtttgc tggtattcat tagttagata
     5341 tgctccacta acatctctca agagttcctt ttccccaatt ggactattag atagaaatcc
     5401 tatagcttgt acgaccatat ccatagagta ttgggtacca cttgtagtga ctattttttc
     5461 aatctttttt cttcctaaat attttttagg actttctgat agacaaagat gtattccatt
     5521 ttttgatagt atttcttcaa ctttatctga aaaactacgg tcgtaataag tcgacaacac
     5581 tctatctgca acatcgaaaa gatatacttc tttccctctt tttcttatgg cttcagcaat
     5641 ttcaactcca atatatcctg ctccaataat tcctactttt ttaatagtac tatcatccaa
     5701 agcttt
//
LOCUS       DAFGFZ010000147         5691 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000147 DAFGFZ010000000
VERSION     DAFGFZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.149"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(490..1299)
                     /locus_tag="LR726_002677"
     CDS             complement(490..1299)
                     /locus_tag="LR726_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL6217704.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1299..2045)
                     /locus_tag="LR726_002678"
     CDS             complement(1299..2045)
                     /locus_tag="LR726_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217705.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2063..2530)
                     /locus_tag="LR726_002679"
     CDS             complement(2063..2530)
                     /locus_tag="LR726_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217706.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2530..2940)
                     /locus_tag="LR726_002680"
     CDS             complement(2530..2940)
                     /locus_tag="LR726_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL6217707.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2951..3964)
                     /locus_tag="LR726_002681"
     CDS             complement(2951..3964)
                     /locus_tag="LR726_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL6217708.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4181..4915
                     /locus_tag="LR726_002682"
     CDS             4181..4915
                     /locus_tag="LR726_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6217709.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4899..5609
                     /locus_tag="LR726_002683"
     CDS             4899..5609
                     /locus_tag="LR726_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6217710.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1833 a         1195 c          943 g         1720 t
ORIGIN      
        1 taaagcgaca aaaaccacga gcaggtccat tcatcctcaa acctcggtga tcttcgcggt
       61 ttaacaccat cagaagaaat gcacaaaaat aataaggaga gtctgagaca taaataaatt
      121 cccagacttt tagtgagtct aaatccgaat aaacggtggg tcgaaagcaa ctctagctgc
      181 gcgtcgatcg cagcaatctc taggtgctat gttcggataa atggtggtca aaagtcagat
      241 tttcggccat ttcgtaacag ccaaccagac aaaaagcgtc tgttttgtct tctactccaa
      301 ttgctcaaat cttagcgact tttggcttgg tcttactcta aacagtttgt aagcctaaac
      361 tttatttgct gtttgtttct aagatcaaag caaaactact ttttagttgg ttacttttac
      421 aagttctttt tactgttgaa taaatgtaaa tcttaagaac aaatatttac ttctataatt
      481 ccaagtttct taaaaaattc cgccaagtgt acctaaaaag cataaaacaa aaattcctag
      541 aataattgtg atcgttttat gccctttgga gtaaagccag acgcataaaa aggtaattcc
      601 taatggcagc aagcccggaa aaatttgatc aaaaatactt tgtaatgaag tttcagctcc
      661 accaaccgtg aattttagtg acgtatttaa agtaaccatc gtgcctatca tcgcgccaat
      721 taccgttaaa cctaaaatcg aagcaccatg agataatttg tcaatcatat ggttgtcttc
      781 tgtatcatca ataaatttag ttcctaaaac atagccatat tttgtcgcca accatttaaa
      841 aataaaatga atggcagtat acaaagtaaa ataaaggatc atgcctaaaa tattcccttg
      901 ttgagcaaaa gaaataccta tgccagccgc aataacccga aaagttcccc agaaaaatga
      961 atcgccaata cctgataaag gtcccattaa tcctgttttg attttattaa ttgcgtctgt
     1021 atcaaattca ggatttttag cattttcttc ttccatggtg acggacaagc tcataacaaa
     1081 aggcgcaata tgcggtgtaa tgttaaatgt ttccgtatgg cgctgcaacg ctgtataata
     1141 atcatcatcg ttcggatata ttttacgtaa ggaacgttca ataccattca tttgtccata
     1201 tgccatcatt ttatcgaagg accaagagga ttgcataaaa aaggagcgcc aaaatatact
     1261 tcttaattct tttttcccaa taacactttc ttttaccatt ataaatcttc ctcctcttca
     1321 aaagttagct tttgaacagc cagtaacgct aagcaaagtc cgacgacagc cacaccgaca
     1381 acatccattt tcagatacat tacgccgaca aatcctaaga aaagataagg catcatcgct
     1441 ttattcgcca tcattttaat taataacgct agcccaacac aagaaagcac accggccgca
     1501 acatcaaagc tgtccaatac aacctgagga atcaacttga tcatcgcatt gattgtagac
     1561 acaccaaaat aagtagctac aaaaactaaa atggccgatg gaaccccaac gattaaaggt
     1621 tgagccacat aatgatattt ttgtgctttt ttgaaatcat gattgactaa tgcctcatcc
     1681 attttgttgt taaaatacgt aattggcatt gcataggcga agttacgcca catctgaaga
     1741 aagatggaca aaggtaaagt caacgcaacg gcagttccta ctccttgacc cgattgaatc
     1801 gcaattgctg tacctaaaac actggaagca taaacttcag ggggcgtcgc actccctacc
     1861 ataattgagc ccatgaacat ggattccaag gtggcaccaa taataattcc tgactctaaa
     1921 tctcccaaaa tcaaaccgac aaaaggcgct gtaaatagcg gtctacccat catcgatgca
     1981 ccaaatgtgt attcatcaat agacgccaaa aaagctacca gtgcgattaa tattgcttgt
     2041 atcatattct tcccctcctc ttttagtcat gcaaaggatt gatttttttg tccgttggta
     2101 cagcctgaca ttgaactgtt atcccgtttt cagtcatttt tttcaccaag tccagttctt
     2161 gttcattcaa gtaaacggaa ctagtaatca ttttccgatt ctcgtttttc tttaaatttc
     2221 ctaaattcac ttctttaatc tcaggtacgt gctgagtaat ttcgtaggca tcctccaccg
     2281 tcccgacaat taaaaatact tggtatttct tgattttttc tccatttaaa acagagatgg
     2341 cctctgtcat atccttcacc acaaattttg tccctgtagg aacagccatt tttagtgttt
     2401 ttttgcgcag attattggta gcgatttcat tacttgcaac gaaaatacaa tttgcaccta
     2461 agttatttgt ccaggcaaaa gcaacttgcc catgaacaaa acgttcatct actcgtaata
     2521 atttaatcat taaaaatcat cctcttctgt tttctctagc aactcgttca ccaaaactat
     2581 cccttcattc ccgctattca tagctttttg gattagtttt ttcgtgtcct gttcacctga
     2641 taagcaaagc tctaaaagca gcggcagatt tacaccgctg attaaataaa aattgtcttt
     2701 accgatttgt tcggagaaag cgttagacac gcttcctccc agtaaatcta ccgtcacaat
     2761 taattcgtca tcaggcgcta agtcggaaaa tagctgctct atttcctggt taatggagta
     2821 gtcactatct atatacgcag ttattgctaa aagtgatttt tgaggaccaa taataaattc
     2881 taaagtatcc tttaagcctt tagccatcat ggaatgcgtc gcaattacaa ttttcctcat
     2941 agctcttacc ttaatattcc acagtccgat aataacgacg aatatctagt gaatgatccc
     3001 gcaccttttc aaaatgagca ctcactcgtt ctaaggccgt tgccataacc attggcgcta
     3061 catactgacg catagcgctt ggtacacctg ctaattcgaa atccgcagtg tcaaaaaccg
     3121 tcacaacatc gctatattta tttacaaaac gctctacgcg atcgactagc ggacgcgttt
     3181 gatcttcacc ttttaataac acaaaactca tagatttatc agtcatttca atagtcccat
     3241 ggaaaaattc ggctgcatgg atcgacttgg tggcaatcca ttgcatttct tccagtacgc
     3301 acatagcata ttcataggca gccccataag tattcccggc tccaacaaac atatgaaatt
     3361 ccgtattttt atgtttttct gcaaattcaa gtgctttctg atcattttgt tgccgaacac
     3421 ttagtaatac ttccggcatt ttttgtaatg tttgaataaa ttcatcatat tcaggaaact
     3481 caccgttatt tttcatcagg cgtgcaccca aagcaaaaaa ctgtaactga agttcttcta
     3541 ctaatgtatc gttgtcagca tcattttcaa tcgcataatc cacgatttcc ccgagtggtg
     3601 tagcagcatt tcctacaaac gcaatcgtcg ttgcaccttg actattggca tacttaacaa
     3661 catcaacggt ttcttgagtc gtaccggtta atgaagatgt gattaacact gtatctttat
     3721 taatcttttt cggctgtgaa caaaccacct ctgcggctat ttcatgataa taatcaagct
     3781 ctgaatgagt gcttaacaga taagctagcg ggctgattgt ggcatatgtt ccaccagagc
     3841 ctaccaaata aagattttta aatcccttct cactgattgc atctgccacc tgattgattg
     3901 tctcgatatt ttttactgca ccatttaata ctgatagaaa acgttcttcg ctaaaaccta
     3961 acatagttaa aaacctccaa gtcaataatt tgaaaaatat atttgcaaat gccggcacat
     4021 taacaaacca cttaaccttt tttatatacc acctgttaac gtttttaatg taccacttat
     4081 agaaaacgct gtcaatagtt tttaaccact tttattttta tttgtaccaa aatccttttt
     4141 tcatgtataa taaacaaaaa tagactctgg agtgtaacgt atgaatactg aagaactaac
     4201 gaacaaatta atacaagaca ttgaacaaga aaaatataaa tacgcgcaag ctattccttc
     4261 agaacgtgat tttgttcaaa tttatgaaat gaatcgttca gccgtaaaaa aagccattca
     4321 acaattagta aataaagagt acctactaaa aatacatggc aaaggaactt ttgttaaaaa
     4381 aaatatccat gaacatttgc aaatcaaatt tcatggtttg tctgaattgt tggaacggga
     4441 agggattcag cctagcagct taattttagc ttcagaaata aaaccggctg gacacattgt
     4501 cgggaaagct ttacagctgg aaccaactga agaagtattt catttggttc gtttaagaag
     4561 cggcgacggt gagccaattt ctattgaaga cacttatatt ccattaaatc tcatacctga
     4621 tcttcaagct tttgatttcc aaattttttc attttattcc acattggctg cttatcatta
     4681 tcaaattgaa aatatctatc agacaatttc cagctcaaaa gtgagcaata aagaagcccg
     4741 tttgttaaaa accagcttaa atcgtccggt aatcagcctt gctatcactg cggttacgac
     4801 aaatgaacaa gcggttgaat acactgaagt agtggttttg cctgaattcg ccgccatcta
     4861 tactaaaaat gttcatcaag gaaaggagat gaaaatctat gcccaaattg attaatctgt
     4921 tggcttatga cctgattgac aaaattattg cttatattca tgaacatcat ttaacccccg
     4981 gaacaaaact gccttcggaa cgggacctat cagaaatgtt taatgttaat cgaactacct
     5041 taagaggcgc gttacaaaaa ctggtggaca acggcacctt acgggcgcaa accaatagcg
     5101 gctattattt aactgcgcca aaattcgaaa aaaatgccca tcatttcttt acaccgatag
     5161 aagattctct gcttagtaag cagcggctac agctaaaggc aacggctctt tcaccgaaaa
     5221 aagcagcagc tctttgtccg gaactgaatg atttcaaaca gcagcaacca caagcttatt
     5281 tagaatatat tgatgcagaa gcaattagct tgatgttatt aatccctatg gaaacattaa
     5341 agaaaagtaa tttctcggac aatcctttcc aagattttaa aagtttggca tatacgcgtt
     5401 ctcaagaaat ttctttaata actgacaatg acgttgcggc ggacttgctg ggagttgaca
     5461 aagaagaggt tctgttattt gtacgagagg aaataagagc cggtaaggag ttggcggcga
     5521 ttgctttagg ttatactatt agtcgccgct gcaacataca ggttaatgtt gctacgacct
     5581 tgcaatccgg ttctgttgca aagttttaaa tctactatca gataaggtag aataatagaa
     5641 aaagatagca ggaggaatga cgatgagtca ttttaaagga aagcaatttc a
//
LOCUS       DAFGFZ010000148         5646 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000148 DAFGFZ010000000
VERSION     DAFGFZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.150"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..2142
                     /gene="topB"
                     /locus_tag="LR726_002684"
     CDS             <1..2142
                     /gene="topB"
                     /locus_tag="LR726_002684"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL6217711.1"
                     /translation="KATRKDGYFEIQDRLFTGETVITYGFGHLVELDSPDMYDEKWKQ
                     WALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADTIVIATDSDREGELIAWSIIQQ
                     AGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYPKFEEVQARQIADWLIGMNGSP
                     LYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFD
                     AKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVETEKKSAASPRLFSLSSLQSKM
                     NQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAI
                     PTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFA
                     EKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEM
                     VTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANI
                     IETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGT
                     QTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQE
                     VLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEA
                     TIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            2158..2307
                     /locus_tag="LR726_002685"
     CDS             2158..2307
                     /locus_tag="LR726_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217712.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            2381..2974
                     /locus_tag="LR726_002686"
     CDS             2381..2974
                     /locus_tag="LR726_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL6217713.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            2990..3889
                     /locus_tag="LR726_002687"
     CDS             2990..3889
                     /locus_tag="LR726_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL6217714.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            3892..4377
                     /gene="ssb"
                     /locus_tag="LR726_002688"
     CDS             3892..4377
                     /gene="ssb"
                     /locus_tag="LR726_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL6217715.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            4743..5003
                     /locus_tag="LR726_002689"
     CDS             4743..5003
                     /locus_tag="LR726_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217716.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            5444..>5646
                     /locus_tag="LR726_002690"
     CDS             5444..>5646
                     /locus_tag="LR726_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL6217717.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT         2037 a         1005 c         1158 g         1446 t
ORIGIN      
        1 aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgtttac aggagaaact
       61 gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat gtacgatgaa
      121 aaatggaaac aatgggcatt agaacatttg cctattttcc ccaatcaata ccactatcat
      181 gttcctaaag ataagaaaaa gcagtttagt gtcgtcaaac gacagctgca gtcagctgat
      241 acgattgtga tcgcgactga tagcgatcgt gaaggggagc tgattgcctg gtcaatcatt
      301 caacaagcag gcgccaatca aggaaaaacc tttaaaagac tttggatcaa ctccttagaa
      361 aaagaagcca tttatcaggg attccaacag ttacgagacg cagaagagac ctatccaaaa
      421 tttgaagaag tacaagcacg tcagatcgcc gattggttaa ttgggatgaa cggtagccca
      481 ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcttt aggtagagtc
      541 caaaccccaa cactctatat gatttatcaa ttgcaggaaa agattagaaa cttccaaaaa
      601 gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt tgatgcgaag
      661 cttgatccaa atgaaaccca agcgacacaa gaagcatttg aagcctattt aaaagaaaaa
      721 ggtgttcaat taggaaaaca gccaggtacg atccttcaag tcgaaacaga gaaaaaaagt
      781 gcggctagcc cacgtctttt ttctttatcg agccttcagt ctaaaatgaa tcaactcatg
      841 aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg aaaatatcta
      901 agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta tctgttggat
      961 cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc tattcataca
     1021 ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat cattcctaca
     1081 aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca ggctatttat
     1141 gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta tgaagaaaca
     1201 acgatcttga cgcaagttca gcagcttcag ttaaaggcaa tcgaaaaggt gccgctagat
     1261 ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga agaagagccg
     1321 ctacttccta aagtgacaaa aggagaaatg gtgacggtgg atctacaagt tcttgaaaaa
     1381 gaaaccaagc caccacagcc ttataccgaa ggtacgttga tcacggcgat gaaaacagct
     1441 gggaaaaccg ttgatagtga agaagcgcaa tccatattga aagaagtgga aggaatcggt
     1501 acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata tatcaaggta
     1561 gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc cgtggaaaaa
     1621 gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt gttaaagatc
     1681 ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt tgtctctcac
     1741 ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac gttacaagaa
     1801 gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg tccaaaatgc
     1861 caggaacaag aagtcttact ctatcaaaag gtggcagcat gtacgtctga agcctgtgat
     1921 ttcaaactat ggacaacgat tgccaaaaag aaactcacag ccacacaact gaaagagatc
     1981 attcaaaacg gccgaacaag ccaatcagtc agaggactaa aaggtcagaa gggttcgttt
     2041 gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc tgaaaagaaa
     2101 aaaacgaact tcaagaaaag aacacgacgc actacaaaat aatggagggt taatcttatg
     2161 cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat tcgttcagaa
     2221 caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat tgaagaacca gcagcggatt
     2281 caaaaagaaa tcgaattaga tcgataaatt cctttcttgt taatataaaa ctaacatgtt
     2341 atagttgtgt tagttttata ttaactttag gggaagagta atgaaagaca aaatccaatg
     2401 tatttttgat aaacataatg gcaacctatc cttgaaggat gctagaagtg aaggattatc
     2461 gcctatgacg ttagatcgat tagccgaaaa ggataaaata gaaaaggtag caccaggttt
     2521 ctatacctta aaggatgcgt tcgtggatga attgtatctc gctcagagta tctattccag
     2581 agggattttt tctcatgaga ctgcgctcga tctttttcaa gtaggaacat atatccctaa
     2641 aaagatcaat atgatgtttc ctaaaggata caatgtatca aaggagaaat tagatcagta
     2701 tgccattcaa ccgcattaca ctaataaaga aaattttgac ctgggagtca cagaaacaga
     2761 aagcttttac ggcaataaaa ttttagtcta tgatttagag cgaacgttat gtgacatgtg
     2821 gaatccaaga tacaaagcaa gcgtagaggt aaaacaagaa gcattaaaag aatatatgac
     2881 aagtaccagt agaaatacaa ataagctaag aagatacatg gatattttaa aatcaccaaa
     2941 agaaatgact ttgtatatgt tgccacttta ttgagaggag taaagatgaa tgacaccgac
     3001 acaatttaaa gagaaaacaa gaaatttcgc agcagagaaa catttaaacc cacaaattgt
     3061 ccaaagatat tatatgatgg aaaaattatt agaatgtatt gttgaatcgc cttatcatga
     3121 tgattttgta ttaaaagggg gatttctgat tggatcaaaa tacgggttag aaaaccgttc
     3181 aaccgttgat attgatacga cttatcgaaa ttcgaaacta acaaaagaaa agttagaaac
     3241 tgttttttct gaattaacag ctaatcctac aaaagatgga attcgttttg aattaaaggg
     3301 attaacagag acgagggaag ccgattatta tccaggtttt caagcaaagt ttattgctta
     3361 tcttgaaaat gcacgtgtac cttttaaatt agatattact tcaggtgatt ctattattcc
     3421 agcagcattg atgtatgaac acaaattaat gtttgaagat aaaacgattc atattccagc
     3481 ttatcctacg gaacaaattc tagctgaaaa attacatgcc acgtttagtt ttggaaaaga
     3541 taattcgcgc atgaaagatt tttatgacct gtatatgatt ccgaaattag aacgtatcga
     3601 tgagaaaaat ttgtataaga gtgttcaagg aacattcaag gaaagaaata atcaatcggt
     3661 gtttaagcat ttgtatgatc aggaaatgcc tcaattggtg aaagatgagg gcttgagaga
     3721 aaagtggaaa agataccaac aagaaaacta ttttgccaaa gacatttctt tcgaatctac
     3781 actaacttcc gttgattccc tgatgcaatc aattgtagag caagagaagg cagaacgaaa
     3841 acaaaatttt aagagatcac aacaaatgaa tcaagaaatg gagagataaa gatgatcaat
     3901 aatgtaactt tagtagggcg attaactaaa gatccagatt tacgttatac cgcaagtggc
     3961 acagcagtgg cgactttttc cctagccgtt aatcgtaatt tcacgaatca aaacggcaat
     4021 cgagaagctg attttatcaa ttgcgtgatt tggcgcaaac cagcagaaac gatggcgaca
     4081 ttagctaaaa aaggcagctt gatcggcgtt gtcggacgaa ttcaaacacg gacttatgac
     4141 aaccagcaag gacaacgtgt gtatgtgaca gaagttgtgg ccgataactt ccaactatta
     4201 gaaagtaaag cagcgacaga aagccgtatg cacgctgatc aatcgtccag cagtccaagc
     4261 cctaccacct ttgaacaacg ggatacagcg acgcctaaca acaatggatt gaacgcttca
     4321 cagaatccat ttggtggaca atcaatcgat attagcgatg atgatctacc gttctaagta
     4381 gcattttcta ttggttgcat ttgctacggt ttatgttata atttagacaa caaaaagaat
     4441 tttcaatcta taaccaaaaa ccactagctg gcgggctagt ggctttcttt tacgtttcta
     4501 ttataagaat taacgggtag gcttactgtc tttcttggca aatttacaat caagccattt
     4561 gcagatatag tgcgcggcta cttctgccat gattgcaaaa acaaaatcaa aaagaatttt
     4621 caaacctata acctcctccc cttgccagat taggccgggg tagcagacag caagactatt
     4681 atagcataat tgaaatatcc aaatgattgg atttttgcta taattttagc gaggtgattg
     4741 tgatgaacaa atatgttggt ttgcttgata aaatccgagt aataaaaacg caaccgttat
     4801 tggttcgctt tactttacaa acgattcatg aatcgattaa ttgtgtagtt gcagatattg
     4861 aaattatcga taaattgcta attatggatg atggaaaata caatattgcc gtcacaggtc
     4921 acttcaataa acgaaatcag ctagttattg ctagcatgta tgttagaaat cccgatcatt
     4981 ttacgcgatc gatgggcata taaataaaag aagagttgta gtcctaaatg aggattgcag
     5041 ctctttttgt tttacataga aacaggaaaa gaccttttta ggaagacaca agtgccttct
     5101 caattgaaaa acctttctct cactccaacc gccaccgccg ttcgagaaga aaggaagtga
     5161 acgctgcacg ttcactaaga tatggtgtct tgcaaagcaa gacaaaatag acaaaacttc
     5221 ctctcaccaa aaggttcgtt cctgttcgtt atctctttat tgatttgcca gaccggcaac
     5281 aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc
     5341 ttgtccttgt cgcttttcgt tgatttattc tggccaacaa atcaataaag aaagaactaa
     5401 cgaaaggaag taaaaccttt cctaagagga ggcagaatca aacatgcgaa agaaatttga
     5461 ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac atactgttga
     5521 atgcgattgt ggagaaagag cgaaatgtat caaaagctat cgtttgtatc aatgtccaac
     5581 atgcgggaaa aaatatacct tgtcttatgg cgattacatt ttgattgatg agaaaggaaa
     5641 gaaacg
//
LOCUS       DAFGFZ010000149         5284 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000149 DAFGFZ010000000
VERSION     DAFGFZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.151"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LR726_002691"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LR726_002691"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="LR726_002692"
     CDS             complement(580..1353)
                     /locus_tag="LR726_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL6217718.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="LR726_002693"
     CDS             1497..1847
                     /locus_tag="LR726_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL6217719.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="LR726_002694"
     CDS             1828..2544
                     /locus_tag="LR726_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6217720.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="LR726_002695"
     CDS             2544..3992
                     /locus_tag="LR726_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL6217721.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="LR726_002696"
     CDS             complement(4062..4571)
                     /locus_tag="LR726_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217722.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..>5284)
                     /locus_tag="LR726_002697"
     CDS             complement(4561..>5284)
                     /locus_tag="LR726_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217723.1"
                     /translation="KIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSAL
                     ANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQ
                     ELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLK
                     TTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLIL
                     SLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1785 a          928 c         1062 g         1509 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttc
//
LOCUS       DAFGFZ010000150         5043 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000150 DAFGFZ010000000
VERSION     DAFGFZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.152"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            160..1875
                     /locus_tag="LR726_002698"
     CDS             160..1875
                     /locus_tag="LR726_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217724.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1878..3641
                     /locus_tag="LR726_002699"
     CDS             1878..3641
                     /locus_tag="LR726_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217725.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3681..4217)
                     /locus_tag="LR726_002700"
     CDS             complement(3681..4217)
                     /locus_tag="LR726_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL6217726.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4540..4962)
                     /gene="mgsA"
                     /locus_tag="LR726_002701"
     CDS             complement(4540..4962)
                     /gene="mgsA"
                     /locus_tag="LR726_002701"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL6217727.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1507 a         1014 c          975 g         1547 t
ORIGIN      
        1 tttttaaaaa aaatagtcta ttcaatttct aaagcttctt cataataaaa ctttcattta
       61 tgaaaaggct tgttctcttt tcattctaaa tttcctaagc tacaatgtgg tagtacttgt
      121 tttttcaatt gtggacaaat aaataatagg aggaatcata tgattcagtt attacgttat
      181 gcaaaagact atcgtaagca aatcattttg ggcccgttct tcaaatttct tgaagctgtc
      241 ttcgaacttg tcctgccttt aatgatggct tccttgatcg ataatggact aaaaatgaat
      301 gaccgaggaa aaatcattga aatgggctta tggatggtcg ctatgtctgt gattggtctg
      361 atttgtgcta tcatttgcca atactatgct tcagtagctt cacaaggatt tggtacagag
      421 ttacgtaatc agctaatcaa aaaaatcaat actttctctc acaacgagtt gaatcatttt
      481 ggaacagata ccttgatcac ccgaatgaca aacgacatca atcagcttca gctagcttta
      541 gcaatggtca ttcgtttatt gatcagagca ccgtttttaa gtatcgggtc agtggtcatg
      601 gcttttgtga tcgactggga agtaggtctg tttttcttag cattattgcc tattttctca
      661 atcattttat tttttatcat caaaaaaacc gttcctctct accaaaaagt gcaagaaaaa
      721 ctagatcagc tcaatgaaca cgttagtcag aatcttagcg gtgttcgtgt catccgcgca
      781 tttgcgcaga caaaaaaaga aacagagaag ttcgataaaa gcacaaatga tctagcgaac
      841 aattatttac gtgtttccaa tatctctgcc ctattatcac ctgctacaac cttgatcatg
      901 aatgtcggga tcatctgttt gctgacagtt ggcgggatca aagtaaatat tggtagcttg
      961 caacaaggac aagttcttgc tttgatcaat tatatgaatc aaatgctttt ggcactgatc
     1021 gttgtttcaa acctcgtcgt gatcttcaca cgtgccgaag cttcaggaaa tcgggtgaaa
     1081 gaggtgctag acacagaaaa ttcgattctt gaagctgagg tcagtactac tcccgatttt
     1141 tcttctgaag caattatcgc ttttgaccat gttgacttcc gttatactcc tgattctggt
     1201 ctttctctcc aaaatattac atttcagctg aagcgaaata cagtccttgg aatcattggt
     1261 ccaaccggaa gcgggaaaac gactttgaca caattgattc cgcgtttcta tgatgtcagt
     1321 tccggtacag ttataatgga cggaatggat gttcgttctt ggcagctgga tcatctccgt
     1381 cagcaaattg ctcttgttcc ccaaacttct gtactattta ccgggactat tcgtgaaaac
     1441 cttcaatggg gtaagaaaga cgccacagaa aaagaatgtt gggaagctct gaaaatcgct
     1501 caggcagaag aatttgtccg tcagcttcca aatggtttag atacaaaagt catggaaaat
     1561 gggaaaaatt tctctggcgg acaaaaacag cgactgacga ttgcaagagc tttgattgcc
     1621 aagccagcat tactgatttt agatgactct ttaagtgcgt tggattatca aacagacttg
     1681 gatttgcgta aggcgctaca gacttatctt ggcagtacag tgattattat ctcccaacgt
     1741 gtccgctcta ttcaagaagc acatcatatc ttagtgatgg atcaaggtaa gattgctgct
     1801 cagggtaccc atgaagaatt acttgctcaa tcagcagaat atcaggaaat cgtcgcatca
     1861 caggaggaag aatagttatg aaagtaaaaa aattatcctt gcagacgctc cgccgattca
     1921 ttccttacct tactgcctat gcaaaagaca tttggctagc tgtgatacta ggaatcatca
     1981 atgggattgc aacagttgtg atgacgttac aaattggtca aagtatcgat caaatgattg
     2041 gagaaggaca ggtccgcttc aatcgactgt tccatttact tattctattc ggtggtatcg
     2101 ttttaatcaa cgtagtcagt caatggctca tccaagtgtt aagcaatcgg ttagcttact
     2161 tatccgttgc aaaattaaga aaagatgcat ttgctcatct gaatcgattg ccacttcgtt
     2221 attatgatca aaactcccac ggaaatatcg tcagtcgatt tacaaatgat atggacaata
     2281 tttccattgc aatttctgcg gccttcaatc aattattttc tggagtggca atcatcattc
     2341 tttcgtttat ttttatgatg cgtctaagtc cacttctgac attggtcgtt ctttgttcta
     2401 caccaattat tttccttgtg agttggatcg ttgctcgtgc ttcacaaaca aatttcgata
     2461 gacagcaaag gattattggg gatatctcgg gctttgtgac cgaacgaatc ggtaaccaga
     2521 aaatcgtgaa agcttttcag caagaagaag ctagtcaaca gcaatttgaa ctgctaaatg
     2581 ctgatctata tgaaaaagga caacgggcac aattttcttc ttccttgaca aacccgtctt
     2641 ctcgttttgt agatcacctt gcttatttat cgattggttt agttggcggc ctattggcat
     2701 tgaagagtgg ttcgtcaatc actgttggtg tgatttccag ctttacgatc tactcaagtc
     2761 agttttctaa accatttatc gaactttctg gaatcacgac acaactgcaa accgcttttt
     2821 ctggtctaga acgagcattt gatttgatcg atcagccaga agaaaagcct gatttgccag
     2881 atgcctatgc cttggaaccc aaaaatatca aaggaaaagt ctcttttgat catgtttctt
     2941 tttcttatga ccccaagcag cgtttgatcg aagatttcaa ttttactgct tatccaggtc
     3001 aaaccattgc tatagtagga aaaacagggg ccggaaaatc cacacttgtt aatttgctga
     3061 tgcgttttta tgacacaaca aaaggaacaa ttacagtcga tgaccatgac atccgctcta
     3121 tccaacgtga tacattacgt aaaagttttg gaatggtgtt acaagatact tggctatttg
     3181 acagtacatt gcgagaaaat cttcgttttg ggaatccgga agcaagtgac gcagaaatca
     3241 atgatgccct aaaagcctct tatatgtatg aatttgtaga acgtttgccg gaaaaattag
     3301 atactaagat tggcggtcaa gggttaaaga tatcagatgg acaacgccaa ctgttgacga
     3361 ttgctcgaac gatgatcagt aatcccccta tgttgatctt agatgaagct accagctcag
     3421 tcgatacatt gacagagaaa aaaatccaac aagccttctt agcaatgatg gaaggaaaaa
     3481 ccagttttgt gattgcccat cgcctgtcca caatcaaaaa tgcggaccag atattagtga
     3541 tggatcaggg agctattgtc gaaatgggca cccatgatga gctgcttcaa aaagatggtt
     3601 attaccgacg gctctatcaa gcacaattca cagcacaata aaaacaagca gtcttctggg
     3661 atgggaagac tgcttgtttt ttatttagcg tggatcgcta aacttcttaa aatttttttg
     3721 taaggaatac tacgtcctac gtaataaatg gcaattgatt gaatcggtaa ccatattact
     3781 gtttttaaca ggcggggcgc ccaaacgacc cagctgaaca atggcacatg gtacatcaac
     3841 gcaagccata atggtgtcaa aaataagttg atgatcagcg tgacactaag cgtagccaga
     3901 atggcatttt tccaagtgat ctcttttcga tagaaaaaga atccgtagat tgctccttca
     3961 ataaatgcat tcaatgtaaa tccaataaag aatgcagatt tcgagaagag ggccattcct
     4021 actaaatcag ctaaaactgc gcctatccct gaccaaaaag gaccaaaaag aatgcccatg
     4081 atcacttgtg gaataaatgt gacactgatt ttaataaatg gggtctcgat tgctaccagt
     4141 cgggacaaag tgaccatcaa tgcaatcaat aagcccataa tagcaatcat acgtgcgtct
     4201 aaccggtgtt tcttcatttt tagcatctcc tttaagagct gctacatcgt atcatgcagc
     4261 gaatgcgaaa ataaaaccgc gaacattacg tccttcgtcc aagaggcaac atcccatcct
     4321 cttagcactt aacgctttat tttctactct gtattatttt tgggatcttt tcccacagaa
     4381 actatagcat attttcacat ttactgcaaa tacgaaaaac taatattatt gtttatatta
     4441 tccaatacgt tcgtattata tcgcatatta tcagtttgtt tagcttttat agctacatat
     4501 aggcgaacat taaacgttat ttttccatac gcttatccat caaaatgaaa gtggatgctt
     4561 gtctatttca tggacgattc ttcgaaaatc taggaagccg gcttccagtc ctcttagtag
     4621 cacctcagct gttccgatat tcgtagctaa cggaatctca tagacatcag ataaacggat
     4681 aagtgctgtt acatctggtt catggggctg tgcagccagc gggtctcgaa gaaaaatcac
     4741 caaatctaac tgatcctccg aaatcatggc tccaatttgc tgatccccgc ctaacggtcc
     4801 ggatttaaac cgatggatcg gcaagccggt tgcttccgtg atcctctgcc cagtcgtccc
     4861 ggtagcaaaa agttcatgtt tttccaatat tgttttatac gctgttgcta atttcaccat
     4921 cataggtttt tttcggtcat gagcgatcaa agcgattttc ataagagaca ctccttttga
     4981 tattgttaac atacttattt actccagtgt atcagttttt atttcttatg gatatttatt
     5041 aaa
//
LOCUS       DAFGFZ010000151         5032 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000151 DAFGFZ010000000
VERSION     DAFGFZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.153"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(630..1442)
                     /locus_tag="LR726_002702"
     CDS             complement(630..1442)
                     /locus_tag="LR726_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217728.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1605..1712
                     /locus_tag="LR726_002703"
     CDS             1605..1712
                     /locus_tag="LR726_002703"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217729.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1770..2837
                     /locus_tag="LR726_002704"
     CDS             1770..2837
                     /locus_tag="LR726_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL6217730.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2979..3182
                     /locus_tag="LR726_002705"
     CDS             2979..3182
                     /locus_tag="LR726_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL6217731.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3242..4444
                     /locus_tag="LR726_002706"
     CDS             3242..4444
                     /locus_tag="LR726_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6217732.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     misc_binding    4806..5032
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1681 a          787 c         1048 g         1516 t
ORIGIN      
        1 ttattagaga aaaataattt gagaggtaga tagtttttat tggaggaagt aattatgtat
       61 atttttgaaa caaaaatttt tcattgcaat aattgcaaca aagaaatttc atcgggggaa
      121 gaggtttgga caaaaatgga aatttccgcc aaaagtaact gctcctcaac tgaaatcaat
      181 taaagaacta gaatttgaga atgcactggt aatttgtatg acatgtactg atgaagtatt
      241 tcgaaaacat ttttaaaggt tgagatgtat atagatttaa ccaatcattt atctctctcg
      301 gaaaaggaat attaactaac cagcgggtta tcaaaaatag gtaggaaaac tgtacttaat
      361 ggcttatttc gcgctgatta tgagaatatc agtctgtttg attaattttt agggttcaaa
      421 atacagagac aaactccagg tcgggtttcg ccacaagcga attacccgat ctggagtttt
      481 gtctattgca ggaaggcgta gccggactgc aggaataaag cccccggttc taacaggggg
      541 ctaacaaaga acaggaaact gtgacaacta cccattgggg agtgtcctag cagtattttc
      601 tttgtcacgt gcttatcaat tttgtcacct cattttgatg atggatacgc tgcatttaac
      661 aggcgttctt tctcgacttc catcacgaat ggtagataaa gatattgctg ttctagtaga
      721 tattgattgt cctctgtgta ctgataagaa gcggtttctt ctgaatagac caacggggca
      781 attcgcacat cgctggtcaa ttgattaatt gagtatagaa ggatggcttt ctcacctagt
      841 tccttgggta aatgtttccc aaaataggaa taggattttt gatttgctaa atagacgagt
      901 atgcgatcca cactaaagtt ggagaaggta aagggcatca tgtgcagaat atgattttga
      961 ttatcatgtt gcaaatagag ctgggcttcc ttaccaaaat tagttgaacc aagataaagc
     1021 tgcttttttt catcgataca ttggaaagta aactggtctt gttcagttgg tggaatgaga
     1081 tttgtaataa aaaattcaat cattcgatcc atttcttttt cactaacaag gtcagcgtgt
     1141 ttgatttcgt ctactatgag accttcaatc ttaggatcaa gcttacctgc ctttttttgg
     1201 agcatttcta aaatgtattg attctcttga tcaccataca aaagatattc attggtgaca
     1261 tgacctatct ctgcgatttg gtgaagggtt tcggtttttg gaaggttgat cccacgttcc
     1321 cagttattga ctgtacttcg cggtaaatca ccaattagtt tgccaaattt ctccatactc
     1381 aactttaatt cttggcgaat ctgccgaatt cgattgccga ctgcttgctt gtctatctcc
     1441 atgaaaactc ctcctcagga taagttctta aatttagtat acgtgtgtac ttaaaaaaag
     1501 tcaaaacata ttgacttaaa ataaatacag gcgtacaata aaaatgtact taaaataagt
     1561 tcaataggtt ctttgacaag taaatagcag aaggaggttt gtcgatgaac caagttaatc
     1621 aacatgatct cggtgaagcc attcgagttt ctcgagaaga aagggggtgg acacaacgat
     1681 atttagcaga aaaagtgggg atttcgagat agttattatc gaaagtagaa aaaggaacga
     1741 gacaattaag tgaagaaaaa ttgaatcttg tgttggatag tttgcaagaa gaggtcgttc
     1801 cagttaaccg tgttttgatt gattatctaa ccattcactt tttctcgaat caacatttaa
     1861 agctaattga agagattatt gaaatgccca ttgaacgttt tgaggaactg gattatgcgc
     1921 caaagggcta tattggccaa tacgtgtgga atcaagtaat cacgattcgg tattccattg
     1981 acgatgcggt taaaggaact gtgatggagt tttcaggtca aggctgtaaa cacttagcaa
     2041 gatggctaaa aacacgaaaa acaaattggc aacagttttt tcgtaaggta ttagattatc
     2101 aggggaactt tacacggatt gattttactt tagatgattt tgtggggagt ctcagcattc
     2161 cagaacttaa gcataaggta atactaggcc atgtgtggac cacttttcaa gtgagtgaat
     2221 ctcatggcgg tacggatatc ataaacaatg aatcaaatgg tgagaccttg tatttaggct
     2281 caaagaagag tcagtgtcgt ttttgctttt atcagaagga ccatgaacaa cggaagcgac
     2341 gaggaattcc tttagaagag gcaaaagtaa agaatcgctt tgaactacgg tatcgaaaag
     2401 agaaagcgca aagcttggca aagattattt caaaaaccca tgatttaacc aaactgttct
     2461 ttgagttact taatggagca atttgttttt atgaccgtga tccaaatgat ctaggtgcaa
     2521 aagttgatgc caaatgggca gcctttatta gcaattatgg cgcaatcacc atttctttag
     2581 atacaattcc tcaaagtttt gagaaaacta tgagttggtt aattcatggt gtttcaccat
     2641 cattagtttt tattgcacag gtcgatcaaa cgtttcattc ttccttgttg cctttgatta
     2701 ttgaacaagg agaaatgaat ccaagacaga tcaaacttct ccaaaatatg aaggcagacc
     2761 cggagtatta tcaggaggag gttgagtatt atgaacgaaa attacaaagt atatcaaatg
     2821 agaaaacaag ctactaaaaa agcaaagtac tgcttacaca acaagttgtg caacgtactc
     2881 cgcctcgaca agacgagtat aacacaacca tcgaatgaga aacaatagat tataaagagg
     2941 attattttaa aaggagtaaa gaatcaggag gtgagagaat ggaaacaaaa tgtgatttca
     3001 tggtaaatcg tgcaattttg atagagatgg gctttaaacc tagtcaagct gcacgaatga
     3061 ttaaagagag caaggcatat cttgcaagga ttgaaggtat tgatttttat aataatcgcc
     3121 aagttggtgt ggtgccatcc cgagtaattg aacatctttt tcacattcaa gttgcggaat
     3181 aaacagtaca agcgcggtac tattaaagta gcttttctca agtacaggga ggaatggaac
     3241 gatgccaaaa attaaaaata tttaccaaga taaaaagaca aaaaaatggt tcttccgagc
     3301 gtatctaggt atagatgaaa atggaagaaa ggttcagaag actaaacgag gttatgcttc
     3361 acaaaaagat gccaaaattg cttatgacaa gtacatggaa acacatgatt ttaacaagac
     3421 aattcctaat caactgtgtt ctaccaatca aatgactttc gaagagtttt acaaggatag
     3481 gtttgtaaaa tggtacgaga gacaggtcaa aagtcaaaca tatgagaatg cgcaattcat
     3541 tttcgaaaag cgtatgcagt ttttctatca ttttcgtgtt cgagatatta caagtcacga
     3601 tattgaggat tggttatttg aactaagcca gacagagtca cgtaattcac ggaaaaaagt
     3661 taatggcgaa acgttatcaa aatcttatat caatcggatt cgagggcacc taaaaattgt
     3721 attggatcgg gctgttaaag agggggtgat tgctaaaaat cccgttgaag atgtttcttc
     3781 gcttccttct gagaataaga aagttgagtt ttgggaacac agtgagtttc aaaaagtgat
     3841 gaatgaattg aaggatgata agattcaaac ccaccatcgg aagattgtgt atgaaatgct
     3901 cttttatact ggcattcgga ttggagaact tgaagctttg tcttggtcga atgtagattt
     3961 tgagaaaaac acgataacga ttgagaagac attgatttat aatacaaaag atgattggta
     4021 cttttcgact cccaaaacta aatgtgctta tcgaacgatt gggttgggga agaagctttc
     4081 cttaaaactc agacagtggt atgacttgca aaaacgtatt ggaaattttg aatatgttgc
     4141 tcagttggac ggaacattta cgccacctta ttcttttgcg aactggttaa aagaagctgc
     4201 taaaaaagcg ggtgtcaaac cgattaagct tcacgcgcta cgacattctc atgttgcatt
     4261 tctaattgag cagaatattc aaccgctatc tattcaagaa cgattgggcc atgcgaatat
     4321 tcaaatcact ttaggaactt acggtcactt atatgcaaag tcagatcaac aagtggtaag
     4381 tgcgattgat aatgttcaag agggcgctat tctttcaaaa aatgacaatt tattaccagt
     4441 ctgagttttc cacggttcta ccataagaca aataaatgct tttgcaccag tgttttcagt
     4501 cgaattgctc ctgttcaaga tgtatcggaa atagcgagaa aataagcaaa tttgcataac
     4561 tataataaaa aggacttagt tttgatggga aatttttcaa aattaagtcc ttttttatgt
     4621 ggttgaaacg ggtatgatca ttttaaggaa gtgatctaag atgaaaggac gactgaacaa
     4681 ttatcatatt agaaaagctt tattgtattc cacaaacaaa ttactaagaa taacaaatta
     4741 gtttttgttt gttcttattc cctattgact tttctttcaa acatgctatt ctaattttat
     4801 tctaatagta tccgatgaga aggaaagtag acagaaaatg gttcaagaga ggctttggtt
     4861 ggtgtaaaaa gctagaagtt gactgtcgaa aatggcctta gaggattttt tgtcgaaagg
     4921 taggcaaaaa cgaacagtct ttcgttacca agaccactga ttttattgtc aggctgagag
     4981 aaagaatgtt ttttctgaaa aaaggtggta ccacgagtat aatcgtcctt tt
//
LOCUS       DAFGFZ010000152         5019 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000152 DAFGFZ010000000
VERSION     DAFGFZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.154"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            165..1306
                     /locus_tag="LR726_002707"
                     /pseudo
     CDS             165..1306
                     /locus_tag="LR726_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1432..2511)
                     /locus_tag="LR726_002708"
     CDS             complement(1432..2511)
                     /locus_tag="LR726_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL6217733.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2614..3054)
                     /locus_tag="LR726_002709"
     CDS             complement(2614..3054)
                     /locus_tag="LR726_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL6217734.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3111..3521)
                     /locus_tag="LR726_002710"
     CDS             complement(3111..3521)
                     /locus_tag="LR726_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL6217735.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3701..4135)
                     /locus_tag="LR726_002711"
     CDS             complement(3701..4135)
                     /locus_tag="LR726_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL6217736.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4235..5006)
                     /locus_tag="LR726_002712"
                     /pseudo
     CDS             complement(4235..5006)
                     /locus_tag="LR726_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322975.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
BASE COUNT         1832 a          962 c          848 g         1377 t
ORIGIN      
        1 gcttcttgag tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata
       61 ttactgtttt tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac
      121 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
      181 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      241 cagaggagac agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      301 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
      361 cagcgaccat caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag
      421 ctgttacatt tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga
      481 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
      541 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc
      601 cgtatagtca gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg
      661 ttgaagaacc aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa
      721 atccagacgg agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc
      781 aaccagagcc agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa
      841 ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag
      901 ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga
      961 ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa
     1021 caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa
     1081 atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct
     1141 taaaaagtag caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa
     1201 atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc
     1261 aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt
     1321 ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat
     1381 tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat
     1441 atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa
     1501 ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag
     1561 taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt
     1621 cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc
     1681 tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc
     1741 tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa
     1801 gccaaataca gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg
     1861 agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg
     1921 aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt
     1981 ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc
     2041 tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc
     2101 gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg
     2161 ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt tgatgaataa gcatcgggat
     2221 atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa
     2281 aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata
     2341 agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa
     2401 aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata
     2461 aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg
     2521 gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa atgacgttat cttatgagtt
     2581 ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt cttgatgaaa
     2641 attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct gttcaagtgg
     2701 aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg cagcaccttg
     2761 agcagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac gattaatata
     2821 atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca gacacaaatc
     2881 atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca tcacaagata
     2941 ctgggtatag gtaagattca gcggcttgag gaggggagtg tagtgtctaa tgatttcttt
     3001 agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt ccatttttcc
     3061 acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt ctattcacta
     3121 actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg acacacttta
     3181 tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat agatccttca
     3241 atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac ttgtgcactg
     3301 acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc actgttaaaa
     3361 catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc gacgcgttta
     3421 ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgcacttc tccagagcgt
     3481 ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc acctttaatt
     3541 atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa atggattggc
     3601 ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag caaacatata
     3661 caaaaaatat cagagacaaa aaataatctc atcataaaca ttacatatat ttctcaaaaa
     3721 gttgttttct ttcttcaaaa attacttcac gatggacctg atgaagcatc cataaataac
     3781 caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct gtcatttctt
     3841 gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata tcagagacga
     3901 tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa ccaagagacg
     3961 tgttttcatc cagcatatgg aatctcgtac catacaaggt aaaaatgact tcattattgc
     4021 ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt tgatacagtt
     4081 ctagtgcttt caaactatca tttaatacaa aatcgatttc cgttccttta tgcataaaga
     4141 tctcctctcc ttttcagtcg aacagttact agattctttt attataccag ctaaagaaaa
     4201 cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga taagtaaatc
     4261 cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga tcgatttctt
     4321 ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat aaaacctctt
     4381 ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt ccaatctcag
     4441 cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata acagatttct
     4501 ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca atagataaaa
     4561 tagcataaag aaactcttct attccaaaaa taaacagatt aagagaaaca acgaatgcgt
     4621 ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa atgaggggag
     4681 ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact cctaaagcaa
     4741 aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt gtttctggaa
     4801 ctattgcaat gattaaagga aaaaccaagc ttccgaaaag tactttaaaa aaagggtttt
     4861 ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa aataactata
     4921 gctcggtcaa agcctaaagc agcttccagt aatattccta gaccgctgac acctccagca
     4981 gcaatatgat gaggccctaa aaacatattg atagcgact
//
LOCUS       DAFGFZ010000153         5017 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000153 DAFGFZ010000000
VERSION     DAFGFZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.155"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..577
                     /locus_tag="LR726_002713"
     CDS             <1..577
                     /locus_tag="LR726_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL6217737.1"
                     /translation="CVMAPCIIYMVFFCKKNTLKHNILILIFMLYCWKVFELTGTGGL
                     TDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLLPIIWKSYRKIVP
                     VFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLSRVVSLKDKSSTV
                     DYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            650..1579
                     /locus_tag="LR726_002714"
     CDS             650..1579
                     /locus_tag="LR726_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL6217738.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1648..2474
                     /locus_tag="LR726_002715"
                     /pseudo
     CDS             1648..2474
                     /locus_tag="LR726_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2522..3700)
                     /locus_tag="LR726_002716"
     CDS             complement(2522..3700)
                     /locus_tag="LR726_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL6217739.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3798..3896
                     /locus_tag="LR726_002717"
     CDS             3798..3896
                     /locus_tag="LR726_002717"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6217740.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            3927..4328
                     /locus_tag="LR726_002718"
     CDS             3927..4328
                     /locus_tag="LR726_002718"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6217741.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4535..>5017)
                     /locus_tag="LR726_002719"
     CDS             complement(4535..>5017)
                     /locus_tag="LR726_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL6217742.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1572 a          861 c          978 g         1606 t
ORIGIN      
        1 atgtgtaatg gcaccatgta ttatttatat ggtatttttt tgcaaaaaaa ataccctgaa
       61 acacaatatc ctgattttga tttttatgct gtactgttgg aaagtttttg aattgactgg
      121 tacaggtgga ctaacagata ttttttatag acctgataca ggtaggaaat tttctctagt
      181 acagggcagt gttaatcttg taccattcag tggactaaac attagcttta tattaaatat
      241 agtaatgtgt gtgcccttag gattcttact accaatcata tggaaaagtt ataggaaaat
      301 agttcctgtt ttcttggctg gggctctatt ttcactaatt attgaagtct cacaaattat
      361 aactacaaga gcaacagata ttgatgattt aatagcaaat acctttggtg cggttatagg
      421 ctttatactg tgggctgttt taagtagagt ggtgtccttg aaggacaagt catctacagt
      481 agattattta gaaccttata tttatatatt gattggtttt accggaaaat tcttactgta
      541 ttttcctttc tggtttgctt ttgaagtatt atcctgataa ttccatagaa aataatgagt
      601 ttgagttgag aaagaatatt cttgtacaca actctggaaa ggaggaagta tgaaaagatt
      661 cttaaaatgg gggcttgttc ttttagcgtc tttgattgtg attctaatgt gtacagcggt
      721 tgttatttct tatgtgaatc ggccgtatgc aagagctgtt tatgatagga ttaaacgcat
      781 tcttgacaaa gatttttatg accatctata tctaggttct tcatttgaaa taataaacct
      841 tgatacaatg gatattcata gctatatgaa aaaagaccaa gaagaattac ccgcctcatt
      901 gagcaaatta tttacgattg attatgcgct tagtgtccta agtttagatg aacagattac
      961 aattagtcct actgcacttg acatgcagaa agctgactca tctactgcac atcttcaaga
     1021 aggagatatt ttatctgttt atgacattat ctgtgctatg gtcttaccct caggaaatga
     1081 tgcagcttat agtttggcaa tttatacagg tggaaaactt gatcctgatg caaagaatga
     1141 tgaagaacgc tatcaaagct tcatgaaaaa cttagatcgt tatctaagtg agcaaggcta
     1201 taaacatact cattttactg acgctgctgg ctatgactcc aatgctacaa catcagcgga
     1261 agatttgaca aaagtagctg caaagctact aaagaatgat aaatttgcag aaatagttgc
     1321 caaggaaact tatagagcaa aatcaattaa gggaaatgtt tataactgga atactacgaa
     1381 tcaatttatg caacaagata atagatactt tactgaaggt gtaaaaggtg taaaaactgg
     1441 atcgtttgat gggagataca atctatgtgt gtattataca tctagttctg ataaccacta
     1501 tttacttgta agttttggtt cagagagtga tagtaggcgt tatgtagatg ttcaaaatct
     1561 attgcataat ctgccttgaa atttcgtgcc tttaagcgcg tatctaaagt ttctttgaaa
     1621 agtttgagaa tgaagaaagt gagcagtcga aaaacggttg ctcacttttt tgccctaaaa
     1681 tcgaaaaaat ataaaaaagt cctttacaac acgtctcagg aaaaactgct aagtcaattc
     1741 taatatcttg tggagcaaga cttgcacctt ttgatataag agagcttaga gaactaatga
     1801 gcgaagatga actagaactc gataaaattg gagatagaaa aactgccttg tttgtaataa
     1861 tatcagatac agatgataca tttaattttg tagtttcaat aatgtattct caattattta
     1921 atctactatg tgataaggca gatgatgtgt atggtggaag acttccagtt catgttaggt
     1981 gtctacttga tgagtttgca aatatcggtt tgattcctaa atttgaaaaa ctaatagcga
     2041 caattcgttc aagagaaata tcggcaagta taatactgca agcacaatct caattaaaag
     2101 caatttataa agaccatgca gatacaatag ttggtaactg tgactctaca ctttttctag
     2161 gaggaaaaga aaaaacaaca gtaaaagaat tatcagaaac cttaggaaaa gaaaccatag
     2221 acctatataa cacatcagaa acaagatcaa atcaaaaatc ttttggtcta aattatcaaa
     2281 agactggcaa ggaacttatg agtcaagatg aaataactgt aatggatggt ggtaagtgta
     2341 tttaccagct aagaggagca agaccttttc tatctgataa atttgatatt acaaagcata
     2401 agaattacaa attgttggaa gattatgata agaaaaactt gtttgatgta gaagagtatt
     2461 taaaacaaag atagcgtaaa atattttgtg taaataactc gtccttctgc aaaataatgg
     2521 gttactcagt aaacattgaa gctaatgtat ccgtcacttg ttggaagcct ttatggcttc
     2581 tatttaagaa tttttgattg tatgtatcaa agatggatac taggaaacgc tctagtgatt
     2641 cttcattttg aaactgctct tttctgtggc tgtatttctt gatttgctta ttaaaggatt
     2701 caatcaagtt cgtagagtaa atagtcttgc gtatggctaa tggaaaatca tagaaagtga
     2761 gcaggtaatc gttcgaaagg atcgattcca ccatttttgg ataactggtt ttccattttt
     2821 ccgcaaaagc accacgtgct tccaatgccg cctttttaga tgacgcttga tacaccattt
     2881 taaaatcatc acagacttcc ttacgatcat cgacacgcac tttgtgactg atattacggg
     2941 aaacgtgcac acagcaatgt tgaaaacggg ctttggggta gaaccgctgc acggcccctg
     3001 ccatcccctt taagccatcc gtgatgaaaa ggaggacgtt tttcacgcca cgttcctgga
     3061 ggtctattaa aatctcttcc caaatcgtga tagattcagt tggtgcaatc gcatagctca
     3121 acacttcctt tgaaccgtcc gggcgaatgc caactgcgat atgaatggct tccttggcga
     3181 cggttttccg ctttaaaggg atataggttg cgtccatata aatagcggca taacggtcat
     3241 gaagctcacg ttccttaaat gaagtgactt cttccgtaaa tgatttagtc atattggaca
     3301 tggtttgggg cgtgtagtga tgcccataca ttttctcaat caagtctgcg atttccgaca
     3361 tggtaatacc ttttcggaag aggtgaatga cggtctcctc taaggtatca ttcgtccgtc
     3421 tataagcagg aacagtctgt tgcttgaact cgccgttgcg gtcgcgcgga atctggagat
     3481 gaagttcccc gtactcggtc ttgaccgtac ggtcatagga gccgttacga gaattacccg
     3541 tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa agccgttaac tccgttttga
     3601 gaagcgtatt gacagctttt tccaaatgga cacggaataa ttcattcaaa tcgcctttat
     3661 tcgctagagt ctttagaatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt
     3721 gaatgtgttg tggtaactct attctactga agggacttcc ttttttctat ttacacaaaa
     3781 tattttacac tctcggattg aaagaacgcg gtctttcagg agtaaagatg gtcatctcgg
     3841 atgcgcatat agggctcaca aaagctgtga aagaatacct tctttgtgcg agctagcaga
     3901 gatgccaagc acatctcctt cgcaacattt ccgacaaatt ctccaagaaa atctcaagcg
     3961 atatcaaaga agactttaaa gccatcttca aaacggcaag catcgagctg gctggagaac
     4021 gtaaacaact tattatggat aaatatgggg aagacaagaa agtcgcaact gcctgtaaaa
     4081 tattggatga gggcttcgaa gatgccatgc aagtaatggt tttaccagaa aatatccgtc
     4141 aacgattgag aacaacgaat gttcttgaaa gactaaatga agaagtacgc cgtcgtgaac
     4201 gtgttgtccg aattttccca aattgtgatt ctgttattcg tatcatcggt gctttattga
     4261 tggaaaagga tgacgaatgg acttctgcac ctagaaaata tcttgaattt gatcgtaacg
     4321 agatttagca cattaagccg ccaatagtat aatatactgg tgagaagtat caagagacgc
     4381 ttcgcttttt gctcttgaca cttcttacca gcatattgga ccgtagctag gctcaacaag
     4441 gcaaaacatc gtactttgag atttcctttt tacacaagat tttggacttg acacagcccc
     4501 tatggttctt accttgttta tgcttattta aatcttactt tattacagta ctttcaaact
     4561 tcgttttgta agccgggtat tttgatattt cataattatg tccctctttt tgaatatatt
     4621 ctttgatatc tgcttgttca tctacacttt ctttgcgtaa aatacgttcg atcattgagc
     4681 agcagtgcat gtaaagcaag aacaattgat tgtttgaaaa ttcgatcccc attagttttt
     4741 cgagttcttt gacggccttt tgtaccaatc tcaaaacctt cattgcatca aggatcgtta
     4801 aattttctac gatcttgtta atcgagaaat taaagtattg agcacaatta taggcgataa
     4861 atggagcatt tgattctatc acctgttgtt ccttggcgta ttcataaatt ttttctgcca
     4921 aaaaggtttt tcctgaaccg cttacaccca tcaataaaat cggtaatccg ttatgaggat
     4981 acaagacagc cgtcttgatt tgatcgatcg cttcctt
//
LOCUS       DAFGFZ010000154         4953 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000154 DAFGFZ010000000
VERSION     DAFGFZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.156"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            260..1534
                     /locus_tag="LR726_002720"
     CDS             260..1534
                     /locus_tag="LR726_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217743.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1588..3015
                     /locus_tag="LR726_002721"
     CDS             1588..3015
                     /locus_tag="LR726_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBL6217744.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            3028..3900
                     /locus_tag="LR726_002722"
     CDS             3028..3900
                     /locus_tag="LR726_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL6217745.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3995..>4953
                     /locus_tag="LR726_002723"
     CDS             3995..>4953
                     /locus_tag="LR726_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217746.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTL"
BASE COUNT         1691 a          687 c          975 g         1600 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgttataaa
       61 aaatagtgcc attaaaaaag aaaagcgaca caccaataaa agtcatatcc tgtgacatta
      121 tatggcatta tccaaataga ataaccggtt gacatgtaag cggatttctt ttataatttt
      181 aggagtacaa gtccttttct aaaaatgtta accggttatc atgaaagggg atacaaatat
      241 atcgttggag gataaaaata tgtcaaaagt taacgtattt aaaaatcaat cttatttaca
      301 aagttcagct acattattac tattttttgc ttcttggggt gtttggtggt cattttttca
      361 actttggcta acatctgaat caaatggttt agggttatct ggcagtgctg taggaacagt
      421 attctcggca aattcgttag ttaccttaat tttgatgttt atttatggaa cattacaaga
      481 taaattgtat attaaacgaa atttattaat ttttgcttct gtattagcga cacttgttgg
      541 accatttttt atatggatat atgggccatt gctagataac aattttaatt taggcattat
      601 tatgggagcg ctatttttgt cagctggata tttagcttct gtaggagttt ttgaagctgt
      661 gtcagaaagg tttagtcgtt tatttggctt tgaatatgga caagcaaggg cgtggggatc
      721 atttggttat gccttggtag cgcttttggc aggattttta tttgtaaaaa atcctcattt
      781 aaacttttgg gcgggatctt tctttggttc tttactattg ttaaatttat tattttggaa
      841 ccctaaagtt gaacgggaag caaatcaaaa ttttaatcaa gaacaagctg aatcaaatag
      901 tattccttct ttaaaagaaa tgtttgatct aatgaaactg cctcaattat ggacgataat
      961 catctttatt gtttttacat ggacatttta tacggtattc gatcaacaaa tgtttccggg
     1021 attttatact ggtttgtttt caacatcagc taatggtgaa aaaatatatg ggacattgaa
     1081 tgctattcaa gtattttgtg aagcgttaat gatgggaatt gttccaatca ttatgagaaa
     1141 attaggggtt cgaaatactt tgttattagg tgtaaccatt atgtgtgtac gaattggatt
     1201 gtgcgggttt gcctcgacac cattatctgt ttcatgcata aaaatgttgc atgctttaga
     1261 agtaccatta tttacattac caatgtttcg ctattttaca cttcattttg atacaaagct
     1321 atcagcaacc ctctatatga taggatttca gatagctgct caaattgggc aagtgatttt
     1381 atcaacacca ttgggaatat taagagacaa cgttggctat caaccaacat ttaaaattat
     1441 ttctcttatt gtattactag caggcatata tgcattcttt attcttaaac aagatgatag
     1501 agatgttcaa ggggatccat ttattcgagg ataatggaaa ccaacagaat aaaaaacaat
     1561 tttatctaga aagtgagcga atatattatg acaaaaagag ctatagaatt aacaaataca
     1621 cgttatcgtt tgggatatca tgtgtctgca ccatcaggat ggattaatga tccaaatgga
     1681 ttttgctatt ttgatgggta ttatcatgtg ttttatcaac atcatccata tagtgctgaa
     1741 tggggaccaa tgcactgggc acatgctaga agtaaggatt tagttcattg ggaatcttta
     1801 ccacttgctt tgactcctgg agatcaagaa gatgaaggtg gttgtttttc aggaagcgct
     1861 attgaaaaaa atggtgtgtt gtatctgttc tatacaggac atcactatta tggagacaat
     1921 gatccagatc atttttggca aaatcaaaac atggcgtata gtacggatgg cattcacttt
     1981 actaaatatg aaaaaaatcc tgtaatagca aaagcaccag aagataacac acatcatttc
     2041 agagatccaa aagtatggaa acacgatgaa aaatattaca tggttttggg tagtcaagaa
     2101 aaagatggat taggtcgtgc tattgtttat cagtcgttag atttattaaa ttgggattac
     2161 attggaccaa tttctaaagc gaaggaagta cttacagaag gatttatgtg ggaatgccca
     2221 gacttttttg aattaaatgg aaaatctatt cttttattat cccctcaggg aattgaagca
     2281 aatggaaaag attatttaaa tctatatgaa acaggctact ttatcggtga ttatgattat
     2341 gagacagcaa aattcattag aggaaatttt tatgaattag ataagggaca cgatttctat
     2401 gcaacacaaa caactttatc tccagatggt agacgtattg ttatggcctg gatggatatg
     2461 tgggaaagcc ctatgcctga aaaagtagac ggatgggcag gagcattaac gcttcctcga
     2521 gaattagagt taattggaga tcgtttgtat atgaaacctg tgagagaatt agaacaactt
     2581 cgagttggca gcggcgttga aacgacagtt aatttggcag gagaaataat aatcgctcaa
     2641 gatgcttcat tcaaagaagc tctaattgat gtactattga ctaaatcaaa aaatcaagaa
     2701 gtcaaatttt cattaaaaac aaaagatgaa gagttaatca atatttttta ttctaaggat
     2761 aaaaatgaat gtaccttaac tcgtaacggc aaaaatgatc caaggtatgg aaccattcag
     2821 atgtgtgata gattatcact tcgagtgttt attgataaaa gttcggcaga gatttttatt
     2881 aatgatggcg agcttgtttt tacagagcgt tattatacag aaaatcaacc aagtatgcat
     2941 ttgagtgtta gtgaagaagc ttttgtctcg agtacaattt atcatctaaa aaaagatgca
     3001 atttcttacg agtaaggagg ttttatgatg ttaattggta gcatagaagc aggtgggact
     3061 aaatttgtat gtgccgtggg agacgaggat taccgagtac aagaccaaat aatttgtcca
     3121 acgaccaccc ccagggaaac attgaaaaaa actgttgact attttaaaca gtttgatata
     3181 aatgcattag gtattgcatc atttggtcca atagaaattc gaaaaaattc accaaaatca
     3241 ggatatatta cgtctacacc aaaggtggga tggcgaaatg ttaatgtgtt aggttattta
     3301 caagatgagc tgaatgttcc aatgtcttgg acaacggatg tgaacggatc agcttatgga
     3361 gagtacatca tgtccacttt atcgaatgaa aaaataaatt cattagttta ttacaccgtt
     3421 ggtactggcg ttggtggtgg agctgttgtt gacggaaaac tattaggatc gtttggtcat
     3481 tcagaaatgg gccatacgtt tgtaaaacgc catcctgatg atattgattt taaaggaatt
     3541 tgtccttatc atggtgattg tttagaaggc ttagttgcgg gaccaacgtt tgaagcacgt
     3601 tttggaaaaa agggtgaaga cgtttcttta acggatccta tttgggatgt aatggcctat
     3661 tatctagcac aagctgtgat tcaaacaaca ttgattatac gaccggataa aatagtattt
     3721 ggtggaagcg tagtaaatga aaaattgtta gaaaaaattc gtgttaatgt taataaatta
     3781 ttggatgatt acgtggaaat accaccgtta agtaaatata ttacgcgtcc tttaattaaa
     3841 aataatggat cggcaacact aggaaacttt gctttgggct tgaatcaatt acaagaataa
     3901 aacatagaaa aaatatcgat tatcaggtat agtagttcta tagagttctt tttttcgata
     3961 gttaattatt gcattattta ggagagacaa gagaatgcgc ccaaaattag aagatgttgc
     4021 caaaagagca aatgtatcaa aaacaactgt ttcacgtgta ttaaacaatc gtggctattt
     4081 aagtcaaaaa acgattgata atgtttataa ggctatagaa gaactcaatt atcaaccgaa
     4141 cgttgtagcg cgtcaattat ttcaaaagaa aactaacatt gtcgggttac tatttcctac
     4201 agtagccaat ccttttttta gtgaactcgt tgaggcattg gaaaaaaagt tatatgaaat
     4261 tggttataag gtattaatag gcaattcgat gaataataaa gaaaaggaaa caaattattt
     4321 aaatcagtta ttgtctgatc aagtagatgg tttaattgtt ggtacacata accaaggaat
     4381 tcaggaatat aaatatcaga atttaccgat tgttgcaatt gatcgagtga tgaatgaaga
     4441 tattccagtt gttgaatccg ataactacaa tggtggaaaa cttgcaacca agttattaat
     4501 tgcacaaggt gcaaaaaata ttattcatac aaacggtcca attgacttac agactccggc
     4561 taatcgtcgg agactagcct atgaagatac tatgaaagca tatcagttaa ttccacgaac
     4621 tgtgacatta gatttcaata tttcttatgt aaagaaaaaa cagatttttt tccaaatgtt
     4681 tgaggattat cctaaaatag atggtatttt tgcttcaaat gacatcgatg cggccctaat
     4741 attacaagta gcaaaagaaa aggggttaaa tgtaccagct gatttattag tagtcggata
     4801 tgatggtact ttaatgactc ggagtatatt accagatctg acaacagtaa tacaaccaat
     4861 taatgatata gcagacacag ctgttgctat tttgatgaaa cgaataaata aagaagaaac
     4921 aaaaaaggaa tatattttac ctgtcacatt atg
//
LOCUS       DAFGFZ010000155         4898 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000155 DAFGFZ010000000
VERSION     DAFGFZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.157"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(488..862)
                     /locus_tag="LR726_002724"
     CDS             complement(488..862)
                     /locus_tag="LR726_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL6217747.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(885..2285)
                     /locus_tag="LR726_002725"
     CDS             complement(885..2285)
                     /locus_tag="LR726_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBL6217748.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2289..2699)
                     /locus_tag="LR726_002726"
     CDS             complement(2289..2699)
                     /locus_tag="LR726_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL6217749.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2717..3208)
                     /locus_tag="LR726_002727"
     CDS             complement(2717..3208)
                     /locus_tag="LR726_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL6217750.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3218..4009)
                     /locus_tag="LR726_002728"
     CDS             complement(3218..4009)
                     /locus_tag="LR726_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL6217751.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4002..4775)
                     /locus_tag="LR726_002729"
     CDS             complement(4002..4775)
                     /locus_tag="LR726_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217752.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1551 a         1000 c          658 g         1689 t
ORIGIN      
        1 gaaaacctcc agacttcttg atagctcaag tatatcttga ggtgcatttg ttagtaaggt
       61 actgttctat ggggcgctta ctctaggagt gagcattttt cgaaaaatac taaaaatttt
      121 tcaaaataaa aaaaggcata cagacaataa actgtctata cgcctcacag actctattct
      181 atacatctaa ttttggctgt cctgcaaaaa atatgtgtta gcctccatcg aaagatcaaa
      241 ctttggaaaa catcattata actttgcttg gtaagagctt tgctaaaaca tgatatgact
      301 tgtatacaaa atgtggagtg tattccaata aataggaagg attacaaaat ttgaaaccaa
      361 ataagtaaaa aatactgtca ctttgaatta atttgaaata gtaaattctc attaaactat
      421 tgaaatgctg ataccaaaaa atgaagtact tttcgttcat accaacttag agcacttcgt
      481 tatctattta tttcttcatt tggcttatct ctataatctc atgatttggt ccgtagaaat
      541 taaagtaacg gattccattt tcccagaacg gtaattccat aatattttta tctaataatt
      601 catatcctaa atcttgaatt gaattgaata cctcatctat gtcatccgta tctaatgcta
      661 gatgatcaat ggctcctcta cactcagtag ttttatcttc ccatatttct attatcgttc
      721 cattcaactc gaaaaagccg actttacttc ccacattcat ttcttcatta atcagactaa
      781 accctaagga ttcataaaag atttttgaag ctccaaaatc ttttgttgga attccaatat
      841 gttgtaaacc atttaatttc acttacatca tcccttcaat tgttttaaaa tccacattca
      901 taatttattg aatagtctag tttatctacc ttgattgctc cctccgtaac agtcatactt
      961 aatgctgttc cgactttatc atacattcta gtacttaaag caacattatc aatatataaa
     1021 acagctatcg tatcatcaac aattaacttt agttcatatt ctttgcctat ttccaaaaat
     1081 agtggtcgtt gcagcccctt atcaaacatt tgaggccaag gatagttcgg agtttttccg
     1141 aaagtaagct gattattaat caatgaaata cgaaattcgt atccttcttg agtatcacta
     1201 tctacaaata attttaaagc aaactccttt gttaacgatg taatagtcaa agttgtttca
     1261 aatagaaaat aatcttcgat tttaggaaca attactcttt ctttttttcc aaattctttc
     1321 tctaacttaa aacttgaaaa tggagtgatt tctttaaaat tctcaattat tttttcaggc
     1381 agtttaacac caagagtttt gtctgtcttt tgaataattt gatgtggaac aagtgcaccg
     1441 ccccaatccc agttattttg atcgttctcc ccttcttttg ttgccaccca gccagttaga
     1501 aaacggccag tttcattagt tccgactgtt cttgctgcat aataggcttt tccgtcgaaa
     1561 gcttcgtcta ctggtgattt ccaatctgaa aataatcctg ctccgactcg atatttagtc
     1621 tttttatctt cacaatactc tgaaaaaagt aaataccacg ttccatcaat ttcaaatata
     1681 tcaggcattt caatcatgtt aaattcatta ttagcccagt agtctccttt gaaattccaa
     1741 tcttttaaat cttcggattc aaaatagacc aatctccccg tcggcttttt cttacttgct
     1801 ggttttctag ctcctagtat caaaacgtat tttccatgat cttgatcaaa aattgcaaaa
     1861 ggatcgcgcc aatcattctt atcatatcct tcttgaggaa caagttttgc tgcttcgcca
     1921 tcctttttcc aatgaattaa atctttagaa cttgcaatca ttaacacttc tgaagttttt
     1981 ccatccaact tagctttgcg attatgtcca gtatacaaag ctaaatactc attgattcct
     2041 tggacaatac ttccagcgta aatgtattga tcaatgtcat cgttgccacc acgatgaatt
     2101 acttccccgt attcttcaaa tttcacaaaa tcagtggttg taactagtga ccacccaaaa
     2161 ggatcagtta aaggtttagg atttctcgta tctcgttgat gaaatagata aaaagttcca
     2221 tctttataaa aaggcatgca atccccaaac caaaaatctt taggcttgta aaaaattttt
     2281 tgcatttatc actcatcctc ttcgttagta tgtctttgca tatctgcaag acacgcaatt
     2341 gtattcatat ttcttagatt ttcgatgata tcatcgcttt tttttccttg taatctcaaa
     2401 ttgacagcct caatcaaaat tcctaaattc atgccaccaa gaagttgaaa cccattttga
     2461 aatacttcac ttcttttagc aaattcgttg aaaggtgttc caccaatcaa gtcagcaaat
     2521 accataactt cttgattttt tacttgagaa actatttttt caatgttttc acggaattct
     2581 tcaatgcctg catctccaag tgttaccaca tgacaattta aattatcgcc aaaaaacatt
     2641 tttaatgtat ctacaaatcc aatggcaata tttccatgag atgtaattat aaactccatc
     2701 cactcatcct ctgttcttaa taaataaaat cctttaaaaa ttcttttgaa tctctaggag
     2761 tttgtatcac tgtcacttca actccatcac taattagttc tttaaaagcc tctctctctt
     2821 cttcagtaat atttatcgtc ggacggatgc gttctgttcc attccttctt gataaatttc
     2881 ctacgtttac acttgttatt ggtaaaccgt tatcaatcaa aaatttaaca tcgattggag
     2941 aaactgcaac aataagaact ttttgtcctt catatttacc agaaagaata tttttaactg
     3001 ctacttccct tgtaattatt gaagttctaa cattaggtgg tgcagcgagg cgaactacag
     3061 ctttttttac gtcatcattg gctacttcat tattaataac cataattctg ttagcactca
     3121 attgatttgt ccattgtgtt gctacttgtc catgaatcat tcgatcatca atacgaacat
     3181 gtataatacc tttcattttt tcatccatat tcaacaccta cttcaaaata tttaacgctc
     3241 ttaacgcaat agaaactact aagattaata gaattacata aactggttta acctttcgtc
     3301 ctaacaaata atacacaatt gctactacac atgcaggtaa taaaccaggc ataacttgat
     3361 caaagatttc atttccggtc attgttaagt cgccttgttt aaattcaaag ccaacattca
     3421 cttttacaac tgtggcaatg agtgcaccga ccaccattaa ccctaaaatt gatactgcat
     3481 cagtaataga ctttaatttt gtttcaattt gagttagtag gctcgcaccc gattctttcc
     3541 cagcaaggta taaaggataa gcagtaatcc ttaataaaat tgatgccagt atccacaaaa
     3601 taattccaac aaaactacct tccaatgcca tattagctgc aattgctcca aaaatagtcc
     3661 atggaataac tacaaataca ctatctccaa ttcccgctaa aggacccatt agacctgttt
     3721 tcagagcact tactgcttcc aaagacttat ttttttcttc tttttcaatt ccaacattca
     3781 taccaataat gtaaggcgct gtatacggcg tagtgttaaa aaattgtagg tgagctaaaa
     3841 cggattgttt cattccttca ggatcatctt tatatgtgtc cttgataaaa ggaagcatag
     3901 aatacgcata acccaaccct tccatttttt cataatttaa tgaagttgca ccattgagaa
     3961 tccaccggaa aattacttgt ttgtcgattt ttgtcagttt cttattcatc ttcgtcaaaa
     4021 cctcctgttg cagctggtgc tacttgagta ttatttgatc tatttttata tacgattagt
     4081 gcgattgcag cgccaagtaa cgaaatccct aaaattggta tttggaaata aactgccaaa
     4141 acgaaaccaa ctaacaaata ggcaaagtaa tcttttgtag gtaaatattt aagtagcatt
     4201 cctatcccaa cagcaggtaa tagtccccct gctaccttta aaccaccggt aacccattca
     4261 ggaatataat tgattgcact tcccactaac gttgggccta atatcacagt taacaaaaca
     4321 ggaattccag atacaaagct tgtattaatt gatgccaaat attgcatcca ctctaatttt
     4381 tgataatctt ctttttgtgc gaatttttct gccttttgtt gaaaaaaaat gtttgttgac
     4441 cactttagaa catctagttg tacaattaac attgctaccg gtattgctaa agttattcct
     4501 atagaagcat cctggttagt tgagattgct aaataagtac cgatcaatgc tgctgtttga
     4561 taatccggta ctgatgcacc accaaaacta gtaataccta gtgtcattaa ttgcaaagtc
     4621 ccaccgatat aaagtccagt ttgaatatct cccataataa gtccggtcaa gaaacctgca
     4681 acgacaggtt gggaggttcc gattttgggc ccttccttat cccagttaat gaaaaatcca
     4741 tacaacacaa ctaaaaatgt ttgccaaaac gccattttgt tctctccttt cttcttatac
     4801 ttttatataa gcaattactg tgccaactaa cattatccaa taaagcggga aaatacagta
     4861 aatattaatt aaatgagcgt ctaaatatac tttggaca
//
LOCUS       DAFGFZ010000156         4859 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000156 DAFGFZ010000000
VERSION     DAFGFZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.158"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(510..1013)
                     /locus_tag="LR726_002730"
     CDS             complement(510..1013)
                     /locus_tag="LR726_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL6217753.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1372..2397
                     /locus_tag="LR726_002731"
     CDS             1372..2397
                     /locus_tag="LR726_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL6217754.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2720..3598
                     /locus_tag="LR726_002732"
     CDS             2720..3598
                     /locus_tag="LR726_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL6217755.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4074..4190
                     /locus_tag="LR726_002733"
     CDS             4074..4190
                     /locus_tag="LR726_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL6217756.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1621 a          772 c          876 g         1590 t
ORIGIN      
        1 tttacacttt tgttatttta tacttaaaaa ataacgacac agaaaatacc gtgtctcaga
       61 tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg
      121 atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca
      181 tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga
      241 gtagggtata aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt
      301 taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc
      361 ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa
      421 tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg
      481 tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat
      541 aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct
      601 aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct
      661 cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga
      721 atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt
      781 ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg
      841 gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca
      901 ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac
      961 cataatagaa agaataaata aatccctaat aaaaattttg atacataccg catagacatc
     1021 tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt
     1081 ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt
     1141 ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa
     1201 aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact
     1261 aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg
     1321 catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag
     1381 acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta
     1441 cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt
     1501 tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg
     1561 aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg
     1621 acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc
     1681 gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc
     1741 atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt
     1801 atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct
     1861 gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc
     1921 attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg
     1981 aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac
     2041 ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt
     2101 gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact
     2161 tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt
     2221 cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg
     2281 atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg
     2341 gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag
     2401 gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag
     2461 gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac
     2521 tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg taactgaata
     2581 aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag
     2641 aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg
     2701 ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa
     2761 aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga
     2821 gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga
     2881 actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga
     2941 ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat
     3001 ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag
     3061 atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat
     3121 ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata
     3181 ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat
     3241 tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg
     3301 tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt
     3361 actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc
     3421 cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg
     3481 acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa
     3541 agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt
     3601 ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca
     3661 cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat
     3721 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
     3781 tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact
     3841 agctataaca atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac
     3901 gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt
     3961 taacaagttg actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt
     4021 cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa
     4081 aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac
     4141 gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa
     4201 acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt
     4261 gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag
     4321 aatctttctt ttcaaattga taccattccc taattccagt tatatagtta actacagaac
     4381 cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac
     4441 ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga
     4501 aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc
     4561 atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa
     4621 gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa
     4681 aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc
     4741 cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat
     4801 aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt tagatacag
//
LOCUS       DAFGFZ010000157         4755 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000157 DAFGFZ010000000
VERSION     DAFGFZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.159"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..145
                     /locus_tag="LR726_002734"
     CDS             <1..145
                     /locus_tag="LR726_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HBL6217757.1"
                     /translation="TIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVH
                     KIK"
     gene            390..1415
                     /locus_tag="LR726_002735"
     CDS             390..1415
                     /locus_tag="LR726_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217758.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            1709..2818
                     /locus_tag="LR726_002736"
     CDS             1709..2818
                     /locus_tag="LR726_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL6217759.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            2901..3797
                     /locus_tag="LR726_002737"
                     /pseudo
     CDS             2901..3797
                     /locus_tag="LR726_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            3813..4637
                     /locus_tag="LR726_002738"
     CDS             3813..4637
                     /locus_tag="LR726_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6217760.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            4669..>4755
                     /locus_tag="LR726_002739"
     CDS             4669..>4755
                     /locus_tag="LR726_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217761.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQ"
BASE COUNT         1619 a          697 c          959 g         1480 t
ORIGIN      
        1 aacaatacga ctaaatccag gtaacaaggg ggcgattgct tttcaccaac tgattggact
       61 ggccaaacaa gaagaaataa aaaatcaagt actactatta gagggagaat tgatttctcg
      121 agactctgtt cacaaaatca aataaaaaag ctctgtgaaa taagtatagt taacaataaa
      181 atatccgaac gatagagaga atgaactgtg atctagcagt tcctcgtcgc aaattcacta
      241 gaattagcat tatttactct aaattgttcg gatatttttt cttgttttta attatgatta
      301 cagtgcctat ttttaacgaa atttgcttat tttcaaaatg tatgattata tgttagtaaa
      361 cactttcaaa tatgaaaaga ggagctatta tggtaggaat tagggatgtt gcaaaacggg
      421 cgggtgtgtc cattgcaaca gtatcgcgcg tgctaaatga ggatccgaca ctttcagtaa
      481 ccgatgaaac caaacgaaag attgcagaat cggttaattt ttatcattat gtcaaaaaaa
      541 gtcccgttgc aaaaaattta acaaatattg cattaataac gacagtttct gaagtagatg
      601 agttggaaga tccctatttt cgaacgattc gcagagggat tcaagtcgaa gcagaacaaa
      661 gcaaagtcaa tttgaagaaa attattcgtt tgtcagaaac accgttggag caagacgatt
      721 tgctaaaatg tcaaggtgtg ttgattattg ggcaggtgct atcttctgtg attgaagaag
      781 tatcagcaat taattcgaat gtcgtagtga ttgatgaccc tagtattgaa gctgaagtag
      841 atgcagttta tactgattta acaaaagcaa cgaatcagca tttagatcga ttgtataaaa
      901 aaggacaccg aaacattgcg tttatcggag gaggacgtgt tcgtttagat agccaaggaa
      961 ataaacacgt gtcagatgat gattatcgaa aaatagccta tgagacgtgg atgaaacaaa
     1021 aaggattaga aaaatatagt caaatttatt tagaaggctg gaccacactt gacggaatgc
     1081 aggcagcaga tttacttttg aaaaaacatg ctaaagataa gttgccgact gctatcatgg
     1141 taggaaatga tcctattgct gtgggggttt atcgttcact tcaaaaaaat ggtctacata
     1201 ttccagaaga tatttcgatt gtgagttttg ataatattga agttgctgaa tttttaacac
     1261 ctgcattaaa tacggtgaat gtgaatacag aagaaattgg aaaactagca gttcgcatgg
     1321 ttaatgaacg aataaaagaa gttagaaatg tttcgatcaa agtgattgtt tctaataata
     1381 ttattgttag agaaagtgaa aaaaatatcg attaaaaaag taaacatcca aaaaagggtg
     1441 tttacttttt tgtttactaa tatttttagt aaaaaatctc gacaaaacat taagtaaaca
     1501 gttatggtgt tgttaacaaa aagaaaacgc attcaaaaca ggaggaatta agatgaaaca
     1561 tttcaaaaaa atagctttaa cagggatagc tgttttaact gggacattac tcgcagcatg
     1621 tggtggggga gatagtaatt ctaaagacgg gcaagtaact ttaagctatg cgagttgaga
     1681 tactgaccaa gctccaggac taagaaaaat gttagatgaa tttgaaaaac aaaatcctga
     1741 tatcaaagta gaaatggaga caacgccttg ggatcaatat tgggttaaat tagaagctgc
     1801 aacaacggga ggtaatatgc cagatgtagt aaccatgcac tctagcgaat cctataaata
     1861 tatgtcacag aatgctttga tgggacttga tgatgttatc aaagaaaatg atattgacat
     1921 gggaaacttt actgaaggaa ttgctgattt ctatacgctt gaagacgaac tatacgcaat
     1981 ccctaaagat gcttctgtag tggggctatg gtacaacaaa gaattatttg ataatgcagg
     2041 tgtcgattat ccagatgaaa catggacatg ggaaacactc agacaagctg caattgattt
     2101 aacagatgaa tcaaaaggca tctatggttt tgcatcacat attggaacag agactggcta
     2161 ctggccattt atttatcaaa atgggggaga agtatttgcc gatgacaata caaaatcagg
     2221 tttgaatacc ccagaagcac aagaagcttt agctttttat cgagatattg cacttgttga
     2281 tcatgcttca ccaacaatta cagaattaca agatcctgat aagttaagtc gttttcaatc
     2341 aggtagatta gcaatgatga ttgaaggaaa ctggtttgca ccgggatttt tgaaaaatga
     2401 ctatctagtt gaaaatgttg cttctgccgt gttacctaag ggaaaagtta atgcaacaat
     2461 tacaaatgga cttggttggg ctgccagtgc gagtacaaaa tacccagaag aagtgaaaaa
     2521 actaatggca tttttagcta gtgaggacgc caataaaatc caagcggaaa cgggggcatc
     2581 aatcccagca ctaaaaggct ttgaagacga gtgggctgct tcttcagata caattgattt
     2641 gagtgctttt ggaaaaatgt tggagtatgg agtccctcgt ccatacaata aagatggatt
     2701 acgagctgaa caagcagaag ttcaaattat taaccgtatt ctgtctggtg aagtaactgt
     2761 tgaagatgga accgctcaaa ttgaaaaaga agtcaatgcg atattgagcg gaaattaaat
     2821 tagttaatta tacaatatac aggcgaaagg attctatttc gtctgtatat taatggatga
     2881 ataccaaaaa gggggatatt atgaaaaatc gaatttttgg aaaatatcgt aaagatcaag
     2941 tgatatgggg ctatattttt atttttccaa cggcattagg attaatttta ttaaatatat
     3001 ggccagcaat tcaaacagca attttaagtt ttcaaaaaac ggtagggttc ggtgatactg
     3061 aatgggtcgg attaagaaat taccaaaaaa tgtttgcaga cggtgaagtt tttcagtctt
     3121 tattaaacac attaatttat gcagtggttt cgatacctag tattgttgta ttatccttgt
     3181 tggtggcagt tttaatgaat agaaaagtta aaggattgtc gatttatcgg acaatttatt
     3241 ttctaccagt cgttgctgca ccatccgcag tcgcaatgat ttggcgctgg atgttcaata
     3301 atgattatgg actgattaat aacatgttgg ctaaaatagg tctcgatgga ccagcatggt
     3361 taacggatcc atcgattgcg atttattcta ttatcattgt aggagtttgg agtgctattg
     3421 gttataacat ggtgttatta ctagccggct tataagaaat tcctaaagat tattatgaag
     3481 cagcggaaat tgatggtgct agtccaatcc agcagttttt caacattacg ttaccattag
     3541 ttacaccaac attatttttt gtagttgtaa caacagttat caatgctttc caagtatttg
     3601 atgttatttt tatgatgatt actcctaata gtaccgccat gtttaaatca caatcattag
     3661 cctatttatt ctataaacat tcatttattt taaatgacaa gggatatggt tcggcaatcg
     3721 ttatgttcct tttagtgatt atattaatta ttactgcaat tcaaatgaag ttgcagaaaa
     3781 agtgggtaaa ctattaagga ggctatcaaa ttatgaaaaa taaatttttt tcaaatgcgc
     3841 caatccatat catgcttatt ttaggtggta ttgcaatggc attacctttt atttggatga
     3901 ttttaacttc agggaaaacg ttggttgaat ctacccaaat tccacctaca attttccctg
     3961 agaaatttca gctaagtaac tatcaacaag tacttgattt attaccattc atggcttttt
     4021 atatcaatac atttttaatg gttgttggac gagtgattgg ctcggtttta ttttcagcaa
     4081 tggctgcata tgcttgtgcg cgtctaagtt ttccagggaa aaatatcctt ttcagcttag
     4141 tacttattca aatgatgatt cccggttcca tttttattac tccacagtat ttattagcac
     4201 aaaaattggg tattttaaat acaattccag cattaatatt accaggggta gttagtgcgt
     4261 ttggtacgtt tttattaaga caatttttct taggtttacc tgatgaattg gaggaagcgg
     4321 cctatttaga aggtgccaat atttggacga tctttttcaa aattatgttg ccgcttgcta
     4381 aatcaggatt ggttgcactt ggaatattca cagcgttgtt tgcatttaaa gatttaatgt
     4441 ggccattgat tgtcaatact tcaattgata agatgccttt agctccggga ttagcaaact
     4501 tacaaggaca atatgccacg aacttcccac aattaatggc aggttcgtta ttagcaattt
     4561 ggccaatagt tgtgatattc atcgttttcc aaagaaaatt cattgaagga attgctacaa
     4621 caggtagtaa attataaaac tattaattag tgattggaga gtaagtacat gacaaaaatt
     4681 acatttattg gtgcaggaag tactattttt gcaaaaaacg tattaggcga tgcgatgtta
     4741 actccaagtc tacaa
//
LOCUS       DAFGFZ010000158         4683 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000158 DAFGFZ010000000
VERSION     DAFGFZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.160"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(448..978)
                     /locus_tag="LR726_002740"
     CDS             complement(448..978)
                     /locus_tag="LR726_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL6217762.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1083..1633
                     /locus_tag="LR726_002741"
                     /pseudo
     CDS             1083..1633
                     /locus_tag="LR726_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
     gene            complement(1786..2259)
                     /locus_tag="LR726_002742"
     CDS             complement(1786..2259)
                     /locus_tag="LR726_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL6217763.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2397..3869
                     /locus_tag="LR726_002743"
     CDS             2397..3869
                     /locus_tag="LR726_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL6217764.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3866..4576
                     /locus_tag="LR726_002744"
     CDS             3866..4576
                     /locus_tag="LR726_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL6217765.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1467 a          850 c          900 g         1466 t
ORIGIN      
        1 tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt
       61 tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
      121 ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga
      181 tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag
      241 cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct
      301 gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
      361 aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc
      421 tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta
      481 ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc
      541 attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt
      601 tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa
      661 agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag
      721 tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt
      781 gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg
      841 gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca
      901 ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa
      961 ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac
     1021 atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac
     1081 tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga
     1141 tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa
     1201 aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc
     1261 aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga
     1321 aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg
     1381 aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta
     1441 ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaac
     1501 gatctttcct actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca
     1561 tgcgatcaat cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa
     1621 ccgaaaaaaa taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa
     1681 cgcagatcac cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta
     1741 gcatctactt attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa
     1801 caactgttcg tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt
     1861 ctcgttgtca tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa
     1921 aatcgtatag cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg
     1981 gacatggtgc attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa
     2041 tccgtcatct aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt
     2101 tctaggacaa ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct
     2161 taattgacgt ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg
     2221 atcattttcc aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct
     2281 cttgcttccc ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga
     2341 atttaggact aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga
     2401 gtaacaacca acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg
     2461 tcttattaat tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc
     2521 ctacattgat gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt
     2581 tcttgctagt caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt
     2641 caagaaattt atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta
     2701 cagcacctaa ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag
     2761 tatcgatgcc attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat
     2821 cagcaatggg gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat
     2881 ctggttatat cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg
     2941 ttgttcttgt cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc
     3001 ctagcattga tattttatcc gctattttat caacgattgg attcggtagt ttactttacg
     3061 gattttctag tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg
     3121 ttggtgtgat tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct
     3181 tggaacttcg tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa
     3241 caaatatggc gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg
     3301 gagaatcagc attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta
     3361 tgatgcctgt gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag
     3421 ggatgttcat tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag
     3481 ttttatatat cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc
     3541 cggtaacgac ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg
     3601 taaataatac attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt
     3661 tgacgaatgt gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag
     3721 cttataaaga tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag
     3781 cgatcatctt tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg
     3841 ttctaatgaa agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc
     3901 ttttgcttta acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt
     3961 cggactaatc ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg
     4021 aatggaaaaa gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg
     4081 agcagatatg cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg
     4141 aacaaatgaa aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt
     4201 agaaaagaca gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa
     4261 cgacatgtat aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt
     4321 gaatacattc aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt
     4381 agctgaatta gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca
     4441 agacggtatg gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca
     4501 acgtacgaaa gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga
     4561 agcaaaaggc gaataatatt ttgaacacca tgtaggaggt tgtgaaaaag ctgctctgca
     4621 tcaagcagta agaacaatca gataaaaata gcactccata tttttcatct gattgggctt
     4681 agt
//
LOCUS       DAFGFZ010000159         4587 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000159 DAFGFZ010000000
VERSION     DAFGFZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.161"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(12..482)
                     /locus_tag="LR726_002745"
     CDS             complement(12..482)
                     /locus_tag="LR726_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL6217766.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="LR726_002746"
     CDS             complement(641..1099)
                     /locus_tag="LR726_002746"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL6217767.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2414)
                     /locus_tag="LR726_002747"
                     /pseudo
     CDS             complement(1270..2414)
                     /locus_tag="LR726_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2427..3344)
                     /locus_tag="LR726_002748"
     CDS             complement(2427..3344)
                     /locus_tag="LR726_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL6217768.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3772..4335
                     /gene="efp"
                     /locus_tag="LR726_002749"
     CDS             3772..4335
                     /gene="efp"
                     /locus_tag="LR726_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL6217769.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1432 a          841 c          870 g         1444 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatctttt ggttagaagt cactaaacca
     1801 gctactacat cgctgtgtcc gcctagatat ttggtaccgc tatggacaac gatatcagaa
     1861 ccgagatcta aaggtctttg aaaataagga gtagcaaatg tattgtcagc aataactatc
     1921 aatccatgtt gatgagccag agtggcactt ttttctaaat cagtaatctt tagaagcgga
     1981 ttgctaggtg tttcaagata aagtgctttt gtattgggtt tgatggattg ttctatttta
     2041 tcaagattac tcgtatctat gatcgtgtac tcgagaccat ttttagtaag aactttgtca
     2101 aatagacgga atgttcctcc atagacatca tctcctaata agatatgatc gccagcttga
     2161 aaaagagaaa aaacggcatg aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca
     2221 ccgccttcca aatcagcaat cagttcttcc aatgcaaaac gagtaggatt accagatctt
     2281 gaatattcat attgtttagg ctggccgacg ccatcttgcc gataagtgga tgtttgataa
     2341 ataggtacac tgacagctcc tgttgtggga tcttcactga tgcctccgtg gatcaatttt
     2401 gtttgaatgt gcatgtttct ttcctcctat aagtaaatgt tttttgataa atagcggtct
     2461 gctgcgtctg gaaaaatcgt aacgacacga tgaccaggtg gtaaacgacg aatttctctt
     2521 aatgctgctg caaaagccgc accactcgaa cttccaacta atagtccttg ttctcgtgct
     2581 aactgcctag tatagttaaa cccttctaca tcagagattg tctcgatctg attgattgaa
     2641 aggggagaaa gaaacggcgg aatgaattct acaccgatac cctcaatttc gtgaggggct
     2701 ggatcaccac catttaacac agagccctct ggttctacac cgattattcg aatgtcggga
     2761 tatttttctt tcaaaaaggt agaagttcca gcaaaggttc caccactccc aattcctgct
     2821 acaaaactat ggatattttc tttgatttca tgaaagattt ctggccctaa cgtatgatag
     2881 taagcagcag gattatcttt attttcaaat tgaagaggca gatagctgtt tgatatctcc
     2941 tctgcaagtt ttttactttt gctgatggca ccgatgattc cttgctcact aggagaatgg
     3001 atgatctttg ctcccaatgc tttcatcaat tgttgttttt ctaagctaaa cttctctgga
     3061 acaacaaaaa ccgttcttaa tttgtatgtg agtgcggcca gagcgatccc aattcctgta
     3121 tttccggcag ttggttcgat gatcgtcgtt ttttcattga tttttccttg ggcaaatcct
     3181 tctttgatca aataactgcc caatctatct ttgatacttc ctccaggatt taaatattct
     3241 agttttgcat aaatagcaga acctttaggc atagaatagt gctgattgtc aaagcgataa
     3301 agcggtgtgg aaccaatagc ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa
     3361 aagataataa aaaaaggagc tgactattaa atcagctcct tgaaagacat agagaaaaat
     3421 tgagtagata aaaagataag ttacctggta ctcattctat gaagggcaat taatagacat
     3481 gacaaatagt atgctgcttt tatgcaccat acaacagcaa cgattcaatt gtttttggtt
     3541 gctgactgtc atgtgtatcc ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa
     3601 taaaagtcaa aggcttgaaa gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt
     3661 tttctgacaa ataggcatga gaccttaaaa gatagagaat actctagttt tctggccggt
     3721 tgtctgatac aataaagttg attatgataa agaaaagaag gagcaaaaat aatgatctca
     3781 gcaagtgatt taagagccgg tatgactttc gtacaagacg gcaaactgat taaagttctg
     3841 gatgcaagcc accataaacc tggtaaagga aatacagtta tgcgtatgaa actgaaagat
     3901 gtccgttcag gtgcaacata cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca
     3961 catatcgaca caaaacctgt acaatatttg tacacgatgg atgatacagc tttcttcatg
     4021 gatttggaaa catatgagca atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa
     4081 tatatcctag aaaacatgga agtaaaaatc caatttttcg gtagcgaagt catcggcgtt
     4141 caattaccaa caactgtcgt attacgtgtt gtggaaacac aaccttccat caaaggtgca
     4201 acagtcactg gttcaggtaa gccagcgaca atggaaactg gcttagtagt caatgttcct
     4261 gacttcatcg aaacagatga attgttggaa atcaatacag cagaaggttc ttatgtaaaa
     4321 cgtgcgtcaa aataattcgc taacggattg ataagaattt gaaaacagag cttgacctct
     4381 tgaaaggcag atcatcggat ctgcctttca agaggttttt tgtggaatca gcaaattgaa
     4441 aaataaaaaa gtatttgatt ttatttatat tttgaaagat gaatggcaaa agctagagaa
     4501 agaaaaaact gcacttattt atgaagaaat tgaaatgatt cataaaattg aaaatatccg
     4561 aactattccg aactatattg aagcgaa
//
LOCUS       DAFGFZ010000160         4466 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000160 DAFGFZ010000000
VERSION     DAFGFZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.162"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            107..358
                     /locus_tag="LR726_002750"
                     /pseudo
     CDS             107..358
                     /locus_tag="LR726_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            451..1245
                     /locus_tag="LR726_002751"
                     /note="aph(3')-IIIa"
     CDS             451..1245
                     /locus_tag="LR726_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL6217770.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1655..1738
                     /locus_tag="LR726_002752"
                     /pseudo
     CDS             1655..1738
                     /locus_tag="LR726_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1787..1870
                     /locus_tag="LR726_002753"
     CDS             1787..1870
                     /locus_tag="LR726_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBL6217771.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1995..2732
                     /gene="erm(B)"
                     /locus_tag="LR726_002754"
     CDS             1995..2732
                     /gene="erm(B)"
                     /locus_tag="LR726_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL6217772.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2995..3858)
                     /locus_tag="LR726_002755"
     CDS             complement(2995..3858)
                     /locus_tag="LR726_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBL6217773.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3860..4132)
                     /locus_tag="LR726_002756"
     CDS             complement(3860..4132)
                     /locus_tag="LR726_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL6217774.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4149..4358)
                     /locus_tag="LR726_002757"
     CDS             complement(4149..4358)
                     /locus_tag="LR726_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBL6217775.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT         1376 a          751 c          830 g         1509 t
ORIGIN      
        1 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
       61 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
      121 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      181 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      241 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      301 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      361 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      421 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      481 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      541 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      601 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      661 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      721 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      781 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      841 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      901 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      961 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     1021 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1081 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1141 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1201 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1261 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1321 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1381 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gttattttgt
     1441 acatttaact tgacggtgac atctctctat tgtgagttat tagtggtaca gttttcaacc
     1501 gttttaatta taaaaaagtg gtgcattttt aaattggcac aaacaggtaa cggttattgc
     1561 aggtgtattt cttatctatg ggtttaacat ggattttatc attaaaatca tgagtattgt
     1621 ccgagagtga ttggtcttgc gtatggttaa ccctaaagtt atggaaataa gacttagaag
     1681 caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag
     1741 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1801 aatgcgttat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1861 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1921 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     1981 tcaggagtga taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     2041 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     2101 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     2161 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     2221 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     2281 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     2341 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     2401 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     2461 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     2521 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     2581 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     2641 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     2701 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     2761 aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa
     2821 ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat
     2881 ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta
     2941 taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat
     3001 acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc tttccaattt
     3061 ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca ttttttgctc
     3121 tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca attctttttc
     3181 taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt ttactccttc
     3241 tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt
     3301 cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg ctgtcattgg
     3361 atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt tgattttagg
     3421 tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg ttgcggtttg
     3481 aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc cttgatcgct
     3541 caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt gttttactac
     3601 gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt taaaggtatc
     3661 attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt
     3721 tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctttttt
     3781 tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt tgtcagtaaa
     3841 attgactata tttgccatat taagccactt tctctttatt caaaacttgt tttgagtaac
     3901 gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa taatcaaaaa
     3961 gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat aattgagagt
     4021 catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa acgcttgccg
     4081 ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc atttcatttt
     4141 cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc
     4201 ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag
     4261 tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta
     4321 tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt ctttctattt
     4381 cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt gattaatcac
     4441 ttgtgatttg tgattctatt cttttt
//
LOCUS       DAFGFZ010000161         4433 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000161 DAFGFZ010000000
VERSION     DAFGFZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.163"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(543..2048)
                     /locus_tag="LR726_002758"
     CDS             complement(543..2048)
                     /locus_tag="LR726_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL6217776.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LR726_002759"
     CDS             complement(2045..2731)
                     /locus_tag="LR726_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6217777.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LR726_002760"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LR726_002760"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL6217778.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAFGFZ010000162         4400 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000162 DAFGFZ010000000
VERSION     DAFGFZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.164"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..184
                     /locus_tag="LR726_002761"
     CDS             <1..184
                     /locus_tag="LR726_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217779.1"
                     /translation="SRNQYLKVLIENDFDHALLNYKKDQFDRLLEKFVDIQTYNTKTL
                     EEYIATNNQLIGLLIE"
     gene            226..648
                     /locus_tag="LR726_002762"
     CDS             226..648
                     /locus_tag="LR726_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217780.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            667..2259
                     /locus_tag="LR726_002763"
     CDS             667..2259
                     /locus_tag="LR726_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217781.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2271..2606
                     /locus_tag="LR726_002764"
     CDS             2271..2606
                     /locus_tag="LR726_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217782.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2753..2947
                     /locus_tag="LR726_002765"
     CDS             2753..2947
                     /locus_tag="LR726_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217783.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2979..>4400
                     /locus_tag="LR726_002766"
     CDS             2979..>4400
                     /locus_tag="LR726_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217784.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKK"
BASE COUNT         1804 a          655 c          885 g         1056 t
ORIGIN      
        1 gagtcgaaat caatacctaa aagttttgat cgaaaatgat tttgatcacg ctcttctgaa
       61 ttataaaaaa gaccaatttg atcggttgtt agaaaaattt gtggatattc aaacttataa
      121 tacgaaaact ttagaagaat atattgcaac aaataatcaa ttaattggtt tgttaattga
      181 ataataaaaa tagtattgtg aatttataaa aggaggaaat caattttggc aaaagcaaca
      241 agaaaacatg tatatcttta cgatatgcat atcgaagtac ttgatgaaat aatttctact
      301 catgaagata tatctaatta tagtgaagca gtcagacatt tgtgtatgat gtatgatcca
      361 aaaatcgaag aaaaaataaa tccaattcaa attaaattga atgcaatggg aaaagaaatt
      421 tctattttaa ccgaaatggt ttcagagttt gcggatcttc atttgaaaga tacaggaata
      481 ctaattggta caaattcaac tgtttatcaa gaagcagaag aacgtgtgga aggaaaaatt
      541 aaatccaatc aaaccaaaaa atattcttct aagttaaaga aaccagtgaa tgaatctgtt
      601 cataccagag tagatccatt tgatccaacg aagattctaa aaagataaaa gtagaaggag
      661 aacgtgatga ataaatcgcc tgcagttatt cttacttgtc aatttacctt gcccaataaa
      721 aaatcttttt caacatacat tgattatatg acaagagaaa aagcactgga agaaatggaa
      781 cgacgtacgc ccgaagaaga acaggaactg caacgaatca agaatgcttt agaggatttt
      841 tatattcctg aaggagaaac tttttcggaa agtaagacgg ataaagaatt cagtgaaacg
      901 tcgaatgaag ctcaatcgtt gatggaacat ggaatcaatt ttgacaagtt agaggaacaa
      961 gattttacca aatatctttc ttatatgacc cgtcactatg cgttagaaca aaagaaagaa
     1021 ctgacgcaaa gggaacaaaa agaataccaa cggttgaacc aagcaatgaa aaaatatcaa
     1081 acagagccac ataaaaaagc ttcaggaaat cttcctggag ttttttctat ggcttcagac
     1141 gaagtgaaag gaagtgatct gaaggagatc aaacaaatct tccaaagagg acaacaaaaa
     1201 ggctcaatca tttatcaaga tgttatctct cacgacaatt cgtatcttga aaaaatgggc
     1261 ttgtacgatc ccaaaacaga taccttagat gaagaaggtt tgaaagcagc tggaagaaaa
     1321 atgatggaga cattattcga gaaagaacaa ttgaatgaaa caggttactg gatggcaacg
     1381 attcaccgta acactaagca tatccatatt cattttgcag ttgttgaaaa agaaaatacg
     1441 cgtaaaccgt atactgagat tgaaaatggt gtgagttata tcgtaccgaa ggggaagaga
     1501 agacaagcaa cattagacgc aatgaaaacc aattatgtac atgagttaga acgtttctct
     1561 tatgaaaagg atcgaatgat cggtatggag aaacgaaatt tattgacacg aaaaagtgat
     1621 ttgcgtaaca ccttaacaaa agtagtaaaa gaaccagcac gttacgatcg acacgccatg
     1681 aacttattaa atgaagtata taaaacccta cctgaaaaac gttcggaatg gaattatgga
     1741 gatgaaaatc gaaccaagct ttcaccattt acaaggggaa aacttgatga gttaaccaaa
     1801 tatgttttaa caaaagaacc tggatatcaa gaatatatac agatttcaaa agcattgaaa
     1861 gaagaagcca aaagccttta tggggaaagc aaaagagaaa caaagaatgc agaaaaaaac
     1921 gccctctttg atttaaaaaa gcgcacagga aatgcgattt taacagaact gaagagacag
     1981 gatagtcaaa taaaaccatt aatagaaagt gtcaatcaga tgcaatctat ttacaatgaa
     2041 cgatcctttc gaacactcaa acagttgcca acaaatgaat acaagtatag ttatcaagaa
     2101 ttcgtaaagc gtaggaaaca attccaacaa agagttgtca cgcagcgaag cttgcgtcaa
     2161 ttaaatcgaa caattaatgc acatgaagaa aaataccacg caagtaaagc atatgaagag
     2221 caacagcgaa gaatccaaat ggaacaagaa agacaatagg aggaagagaa atgaaacaat
     2281 atttagatca atggaaagtc atagaaggat cactaaaaga agaacgaatc gaacaacttc
     2341 ctgattgttt agaaaaagag catttgtttc agataagcga aatgttgaga aacgaacagt
     2401 ttgatccaaa tcaattttta gttgttgagt acccagctac tggtgtgtat tgttgtaatc
     2461 atgtgaaggg tgaaaaatat tttattattc aagaatacga aggtaagtta gcgccttatt
     2521 atactacttg ggaaataaat gaagagggaa tcaataattt cccatgtgaa agcattgaag
     2581 agtctatttc tctcacggaa tgttaaataa atagagacgt aaaagagtcc tgaacgtgat
     2641 tgtccaggac tctttttcaa tgaattgttt tccgccaatt cactgagtgg tgcgattcaa
     2701 ttttattata tcaaaagaaa gtgagattcc aaagtgaagg gaggaagaag aaatgactca
     2761 gacaacgatt ggtgttgtgg ttgaaaaaag cagggataat ctcgtttttg tgacagaaat
     2821 tcaaacaggt agagcttttg tagtcacaga caaagctgca aaagcgtatc aaaatggcaa
     2881 tattttaacg ttaaatctga ctacgaagat gttcgttgat gcagtagaaa attatccatt
     2941 tgtttagaca aacgatattt tacaaggagg aaacggacat ggcagaagaa aaagaaccac
     3001 tagataatag tcggttggga aagtccaaaa gaaaattagt acggttgcaa aacgaattaa
     3061 ataatcaatt tcaaaagcta tttgatcacc aaaaattgac aaatgggcaa ccaatgaatg
     3121 acaaacgaaa tggtcgttca tggtttaacc agcaagaaaa aatagaaaat aaaatacaaa
     3181 gtttgagaga agagatcaag caacaagaaa aacaggtaga aaaacttgaa agacaagaag
     3241 agctaaagga aatgggctat aacaagtacg gcggactgga tatgacgatc gataatattc
     3301 caagaatcaa agaagaaatc gaacgctttg aaaaaggtga atcgactttt tcagccgcca
     3361 cgatccgaaa atatcaacgg aagttggaaa cattagaaca gctgaaagaa aaatcagaaa
     3421 aaggaaaaga aaatatcctc cctgaagtac aagcgatcat tgacagcgga cgagtgacgc
     3481 aatggaaaaa aaatccaacc atttattttt tgaaaggcta tcgaaaagtt gctttagaac
     3541 tgaatgaaaa cgggggattt gaggaatcaa aaaaatacca agccacgtcg gaagaggaaa
     3601 aagcagctgt tcgtgaatta cttgaagagg ctgcaccaac tgattctctg aaggagaaag
     3661 aaaaagatca tttctatcga gtagaattca acgaaaatga tccagaaaaa ggattgaaaa
     3721 gttatggtgg ggagatcgta acattagaat tgatcgatca acttaaaacg ttggataacc
     3781 aatacgctgc agaagcaggt tattataaat tctattttga agaagtgaag aacggagaag
     3841 tagtagatca gatccgaatg gatctagggg atggcgtcaa cagtaatcac cccatctatt
     3901 tagagttagc aaaattgtgc gaacaagtac ctgagaagga acaaaaacaa tcggtcaaaa
     3961 atgaacctgt ttcgaaagaa caaatgcagt cgaaagaaac aaaaacaaaa caaaagggct
     4021 atcaaaaacg aacggcagaa gagatcaaac aagaagttaa atccctttcg caacaggcac
     4081 tggatgcggt gaaaaagcat acccattctt ctgaagacgt caaagaactt ttgaacttta
     4141 tgtctcgttt tccagaacga tcctttagaa atcaattatt gattgaggaa caatttccag
     4201 gtgcaacggc ttgtttaggt cgtgccgcgt tgaaaaaaga aggaatctat atcaaaaaag
     4261 gggaacaagg gaacaagatc tttgtcagaa aaacggtcaa aggattttat gagaaaggac
     4321 gtggctttgt tcgagaaaca gaagctacac cagaagataa aaaacgaatc gaaagtggtc
     4381 aaatcgaagt gatcaaaaaa
//
LOCUS       DAFGFZ010000163         4394 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000163 DAFGFZ010000000
VERSION     DAFGFZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.165"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..637
                     /locus_tag="LR726_002767"
     CDS             <1..637
                     /locus_tag="LR726_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6217785.1"
                     /translation="KKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRI
                     GEILALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFW
                     KLEQRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTH
                     ASMLYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(730..1023)
                     /gene="rpmA"
                     /locus_tag="LR726_002768"
     CDS             complement(730..1023)
                     /gene="rpmA"
                     /locus_tag="LR726_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL6217786.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(1036..1380)
                     /locus_tag="LR726_002769"
     CDS             complement(1036..1380)
                     /locus_tag="LR726_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL6217787.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(1407..1715)
                     /gene="rplU"
                     /locus_tag="LR726_002770"
     CDS             complement(1407..1715)
                     /gene="rplU"
                     /locus_tag="LR726_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL6217788.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1735..1811)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1997..2638
                     /locus_tag="LR726_002771"
     CDS             1997..2638
                     /locus_tag="LR726_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL6217789.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2700..4331)
                     /locus_tag="LR726_002772"
     CDS             complement(2700..4331)
                     /locus_tag="LR726_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL6217790.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1431 a          933 c          757 g         1273 t
ORIGIN      
        1 aaagaaatta aaattatata caaaagaaca gttggaatta tttctaaatg aagtatctca
       61 agaacagaat ccatatttta aaaacagaga ctatacgctc tttagactat tagcattcag
      121 cggatgtaga atcggcgaaa tattagcact cacttgggac aatattaatt ttaaaacaaa
      181 tgaaatggcc attaaaaaaa ctgtagctcg ttcagataaa tattatatat ctgaaactcc
      241 taaaaccaaa aaatcaaatc gaataattta tttagatgaa aaaactataa agcaactaaa
      301 attttggaag ctcgaacaaa gaaagtactt atttcaatta ggatttacta aagctaatta
      361 tttgtttacc aatgacgaaa ataatttcac aattaatcag tcagtggcag aaagatacaa
      421 tatatatcgt gagcgtgccg gcttacctta tatcggtctg catggtttta gacatacaca
      481 tgcatcaatg ctatatgagg caggcgcaga tcacaaagaa gtccaagaaa gaatgggcca
      541 cgcaaatata aaaactacta tggacacata tacacacatt actaacagca aaaaagaaga
      601 aacaacacaa aaactaacaa attatattaa cttctaagaa agtatggtca aaagtatggt
      661 caaaaaaaat cccataattt acaaaaaagc tccatccctt gctacacaag agatagagca
      721 gtttatttat taagcttctt gagctactgg ataaacagac acttgttttt tgttacggcc
      781 tttacgttcg aaacgtacta cgccatcaac ttttgcaaat aaagtgtcgt ctccaccgat
      841 acccacgttt acacctggat agatttttgt accgcgttga cggtaaagaa ttgatccacc
      901 tgtaactgtt tgtccatcag cacgtttagc acctagacgt tttgattctg agtcacggcc
      961 gttagatgta gaaccgccac cttttttgtg ggcaaataat tgtagattca tagataacaa
     1021 catggtctgc acctcctatt tatcattaat tgttttaatt tggatatatt ctgagttttc
     1081 agcttctatc gattgtaatc ctaataaaag attttctaat agaatctcag caatattgtt
     1141 ttgttcttgt gtcattcctg aagtgacttc ggtataaaga taaccgcctt cttcttcatt
     1201 catttcaaca atcggctcaa aaccggctaa tgaggcaatc ccgttgacag tgctgatcgc
     1261 taacgcagaa acacccgcac aaacgatatc acttccataa ggaccagcat cagcatgccc
     1321 ggtcaaagtg aatgaaacga tctgacctgc gtcatttcgc ttaaatgttc ctttgatcat
     1381 atataggcct tctttctcaa acgctattaa gcgttgatcg cgtcgataac cacttttgta
     1441 tatggttgac gatgaccttg tttacggtgt gaatgttttt taggtttgta tttgaaagta
     1501 acgactttct tttgtttgcc gtgtttttca acagtacctt caacagttgc acctgcaaca
     1561 gttggagcgc ctacttttgt agattctccg cctactaaga taacttcgtc aaaaacaact
     1621 ttttctcctg cttcaacgtc aagtttttca acatagattg cttgtcccac ttcaactttt
     1681 acttgtttac caccagtttt aataattgcg tacatgctat aaagcacctc cttctgatat
     1741 acttagactc gccatccgaa gtgacagcat atgctgtgct tattacttgt tctgagcggt
     1801 tgtagctgtg gagtccacaa ttacaacagt actagcttac cagaacagca accgttagtc
     1861 aactaaattt aaaatcaaaa agtaaaacaa aaattcaagc aatacttctg cttttctatc
     1921 ctcacagttc taccaaataa aatagtcgca ttgtgtttct tttattctta taataaaaga
     1981 aaaaggagga gcataaatgg aatacccaaa agaactgata caagaagctt ctttaagaat
     2041 gaacggccgt cctgtagaag gatttatcgc tgggcaagga cctcttcatc ctaaactgat
     2101 gctcgtggga gaagccccag ggaaaacaga gattgatacg catgttcctt ttagcggtca
     2161 agctggaaaa gaattgatgc aggcactttc ttcaactggc ttaacacgag aagaggtgta
     2221 tatcaccagt gctgtaagga gccgcccgta tcgcgtgact catcggatca ataaacggac
     2281 acaacagtca gaaactgttt atccaaatcg gacacctact cgttccgaag tatttgcgca
     2341 tgcaccaata ttggattacg aactgatgaa ggtccagcca aaattaattg cgacacttgg
     2401 aaacattggt cttcaacgtt tacttggcaa agaatatacg gttaccaaaa atcatggtca
     2461 actctataca gggcctgtct tacaattaac agaacaaaag gatgcctacg aatggtcaga
     2521 aaaaaactat cgaattcttc cactttttca tccagcagct attttctaca atcgctccct
     2581 tgtacctgtt atccatcaag actggcaagt actagcttca cttttacagc aatattaaaa
     2641 agaacagaca agaccataaa atcttcagtt tacagtcttg tctgttcttt ttcattgttt
     2701 taatggctaa ttgcagtata tttcgcatgt aaagctgtca acgcatgagc aactgtttga
     2761 tgttgatgcg tgaatgtatg ttcccacaag tgagaaagtt gtttttctac ttctggctgc
     2821 aaggaagtat agataatcgt ttgtcctctt tgctctgctt tttcttgaaa ttcatcaaaa
     2881 actgcacgtg ccgtcaaatc aatacttggt acgcctcgca tggagaaaat gacaccatcg
     2941 tgatgattta gttcttccaa tttatttttg agtttttctg aagacataaa aaacagtgga
     3001 ccactaatat acactactgt ccagttattc agcttttccg tttctggcag ccccattctt
     3061 tgccaatcga tcgtttctaa tgaaatcgtg atagcagcgc ttttgacaac aaaaaataca
     3121 cagccgccga tcaccccgat cacgatagca atacttaaat caaagataac tgtacaaatc
     3181 atcgtaagga taaacaatag aactgctgac caatatttat tagtaaatag ctccttgatc
     3241 gtttcccatt cattcatacg ccaagccgtc acaatcaaaa cacctgcaag tgctggcatt
     3301 ggaatctgtg acatcactgg cgcaaaaaca agcatggaga gcagtaagac aactgcatgt
     3361 atcataccag ctaatctagt ttgtgcacca gatttgatgg ccacgcttgt acgggcgatg
     3421 gccgcagtag caggaatccc gccaaagaaa ggaagcaaca gatttccaat tccttgcgcc
     3481 attaattctt gattgctatc taagggctta tttgccattc ggccagccga agcaccacat
     3541 agcaagcttt caatcattcc taataaagca atgctaatag ctggaactgc cacttgttgc
     3601 aaggcagaaa agctaaaatc tcccatattc aaacgattac tactgatcaa tgtttgtgga
     3661 atcttaccca cagttgctac tggtaaatgg aacaatagca taactgctgt tgccaaaatg
     3721 atagccagca gcgaactagg cattcgtttc gcccattttt ttggatagat aaacatccct
     3781 aaaatgacta gccctcccat gatcaaagag gctgtgttta tcggaaatcc tatcgtttga
     3841 taactactta gtttttcaat aacgtttgta ccttccgagt gaacaccaaa taaattatcg
     3901 atctgaccta atgcaataat gatagcaata cctgaagtaa accccgtaat tacgggtgct
     3961 gggataaaag aagtcagtgt tcctaatcgg aaaagtcctg ctgcaagtaa aaatagaccc
     4021 gctaaaaaag tagcaagcaa tacaccttgc attccctttg ttgccgcaat agacatcaag
     4081 atagctgcca tcgcacctgt cggacctgat atttgataaa aaccgcctga taaaccgcca
     4141 ataacgatac cggcaatgat agccgtaatc aaacctgcgg ccgcatcagc tccgcttgat
     4201 accccaaaag ccaaggctaa aggcaatgct acagcagcta ctgtgatccc agccaaaaga
     4261 tctttctgaa atcttgaaag atcataacct gaaaattctt tttttagtga ggaaacataa
     4321 tttttgaaca cctatctaac tcctttttca tgaaattttc taaaatacca ttcattagaa
     4381 tatcatattt catg
//
LOCUS       DAFGFZ010000164         4380 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000164 DAFGFZ010000000
VERSION     DAFGFZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.166"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(735..1028)
                     /locus_tag="LR726_002773"
     CDS             complement(735..1028)
                     /locus_tag="LR726_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL6217791.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1431..2609)
                     /locus_tag="LR726_002774"
     CDS             complement(1431..2609)
                     /locus_tag="LR726_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6217792.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2713..3027)
                     /locus_tag="LR726_002775"
     CDS             complement(2713..3027)
                     /locus_tag="LR726_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217793.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3134..3349
                     /locus_tag="LR726_002776"
     CDS             3134..3349
                     /locus_tag="LR726_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217794.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3649..3891)
                     /locus_tag="LR726_002777"
     CDS             complement(3649..3891)
                     /locus_tag="LR726_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217795.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3878..4306)
                     /locus_tag="LR726_002778"
     CDS             complement(3878..4306)
                     /locus_tag="LR726_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6217796.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1388 a          832 c          654 g         1506 t
ORIGIN      
        1 attctaaggt tattttagtc ttgtccttta atacaaagat tttattttta aataaaaaat
       61 actctttttt tacgattatt gaaaaaaaaa gataaaactt gattaattga agtattgctt
      121 aaatttggaa gctatgaggg aagaataaaa gagttaggac gggattatct actatttttc
      181 tttgttttaa atatttgtta tctttgaaca taaaagagaa atgtgtcgac tgcagtgttg
      241 tgtccctcat cacgaataaa cggtgttcaa ctagctgatc ttgtattcac ttaaaaaatg
      301 gtaaagtacg agttgctatt ccccattttc aattttctaa cgctcaggac aaaatactgt
      361 tttcacaacc tcattggaat aaaagcatat atattgtttc aagaacgaaa tcttgttatc
      421 tttttaaagg gcatctgtcc aaaatctaat tttaggaggt acgttttggt atttattcgt
      481 acaaataaaa gagaattatc tttattcatg taacgcatcc aattgatttt ttcatgtacg
      541 ctcctggaac gattttactg acgattgctt atttaactgc gaatcgctca ttagctttta
      601 tcatagctat ccatattttg aataacgttc ttggctttgt attatagata cagatgacaa
      661 aaaaagttca agtatgcggt gtagaaccaa tatcgcatac ttgaactttt ttcaatgagt
      721 ataaattaaa acttctaatt ttcaattccc atcatctgat atgcccgacc gtctgaactt
      781 cttttgaaaa agccaaatgt gattagataa cgtctttgtt cgacataatc gccactagaa
      841 aatacgttca gcagtatcga attaacctct gtttctgtat aagtcactcc atggtcaaag
      901 cgtttcaaga cttctttcaa taggatgtat ttcttttttg ttgaacttgg tacttgtttt
      961 acttttccat cattcatgaa tgtgtctaaa actttcatct tttcttgctc cgtataagta
     1021 taaaccatct tctttctcct tttgattcta agaatactcc atttatgtta gaatattctg
     1081 gattttttat taaagcggtt actcttaaga aagatattat catagtgctc ttttttttac
     1141 tactacttat aggtagattt attctaatcc attattctcc cctaccaata attaaagaaa
     1201 aaactggatc agggtgttca aattacatgc taagaaaaaa gaacccttga ataccaaggg
     1261 ttctttctca gtctgataac aatgattaac gacggatttc tttgatactt gtgaaaagtc
     1321 gttaaagtat tgatataaaa ggatttttat tacctctgac tacatattga ctaatttgtt
     1381 aaaaaatcgt ttttcaagaa tcctgtaata ccagtgcttt caccgttttt ttaactctct
     1441 aaagaactaa aagcaatcag aggttttacc ttttcatttg acgttaagtg gcgataaatt
     1501 ttcgaagttg tttggtcagt atggcctaag cgatctttca agacttccca gctacagtgt
     1561 tccgctacta agcttgccat tgtatgacga gctaaatgag tatgaaatgg cttattaatt
     1621 cctgagcgat tcacgcaccg tttgacgtga tcatttacat actctcgacg ataaggaaca
     1681 ccacgtttac tagaatcaag atacacaaac aaatatgttt cttctataat aaacctgtaa
     1741 tgttgcctca tctcacccaa agccttaatc aactgttgta gcttttccat aactagagga
     1801 gttactagaa ctcgtctagc agaactaact gtcttagtag tttcaattcg aaaatcctta
     1861 ctcttacctt taactgccgt ttgcttatag aacagaattt catttctttt gaaatctata
     1921 tctccaggct gtagagcaag cgcctcgctg actcggcagc cagtgtaaaa tagaacatgg
     1981 aaaagtacag acatcggtaa ttcctcatga acaatgccat aattcaaaaa agttctcaat
     2041 tcatcgacag taagaaactt gtcctcaata ttatttatgg cttcttttaa atcagttacc
     2101 gttaactgat attttggcat tctcacagac ttcattggat tttcacttat caaattcttt
     2161 ctttgacaat atttaaaaat catatttaga acttgaataa gtgattgtaa actatttttg
     2221 gcatagccaa catttgttcc attaacgcca cattccttta aggtaaaaag caattcttga
     2281 aggcactctg gtgtcaaatc agacaacttt ttatcaccaa tccatcgaac aataattttt
     2341 aattgttcct ctcgcttatc ataggttatt tccttttgtt catttaacga acggtaatat
     2401 tggaaccatt gctcacctac aacactaaac ttgtcattgc ttgcaacaat ttttttctta
     2461 agcctttgtt gtttctccat ttccagataa gcgagttgcg cctcacgttt tgaattaaag
     2521 ccacgtcttg ttgttcgata tgcttgaaag taccatgtga tcctaccagt agaactgatt
     2581 tgcttcttga atcgatctgt tttttccatt tattgattcc tcctattttg aaggaaccca
     2641 acaagcagaa accctttgga aatctttcta tctatgtcat cattgtaccg catcttagaa
     2701 agaaaatgta acttataaag atcgaagtag tcgcaatttt tctcgatagt cattcgaatc
     2761 actctttctc atccattcta aaacatcatc acgaaaatag tgtcctcgaa tcccgtacca
     2821 acagggagga aactcaccaa gttttactaa acggttcagt gtactatcac ttatctgaaa
     2881 tagtcgcttt aaatcagtat ttgttaatac cagaggtaaa tgactgactg caagcaattt
     2941 ttccgtgtaa tcttccagtt ttttttgtaa aattgaatag aactctattt ccagagaatt
     3001 aacatttgtt ttaacttcca actccatcac ccttctctct caaaatcaaa acgaccacca
     3061 cggtacccct taaaattgta aaaaaacact ccagtactcc atcaaaacgg actagaaaag
     3121 aggaattttt aatatgaaac aaggaattgc agacataaaa ataattaaag aaattcttga
     3181 aaaatctacg gctaatgcaa tagcattcgg aacaggaatc aacttgagta cagtaaaaaa
     3241 acttaaaagt ggagagagag ctgaagaaaa attaaatctt gccgatgcta ttaaaatcac
     3301 agaatttggc atgaaaaata tgccaaccaa aatcgaaata tggaaatagt agtttttctg
     3361 atctatcagc tgttttgcca aagcacatag gaatctcacc tccccccagt aatggcgggc
     3421 cgatttggtt acggaagtat cattatcgta gtcgcgattc tcatattaaa atttgtcgct
     3481 taaaagctaa ccttgtcatg gcgtaactag agaatcaaaa tctacatgta ctgatagatt
     3541 tgctattcat ttgtcaaaga gcgctaggct attttcagcc tatggaaatc ctttattttg
     3601 acccgataaa aaacttccat agaatgaaaa ggaaagggaa aaaatacttt aacgagttgt
     3661 atcaaattca agtatcttct caatcaattt tgtttccagt tgtctacgca tatattcatc
     3721 tacatacata accattcctt tttcttcttc ataaactggt ttcatggcta accgtgaaat
     3781 ataattttga tattttgaca gaacgatgga catggcaatt gaatcaccag aagctgcggc
     3841 tttgattgtc gttagtggta tgaatggtat atatcgttta ttttgcttca tatttgacac
     3901 ccaaatactt tcctagctct tccaaagcca tttgtcgttg gtaccaaaca ctgccgagac
     3961 ttttcccaat aatatcgcca atttcacgat cattaagacc gatgaagaag gacataaaaa
     4021 tgatttcttg tctttgacta ggtaatgctg caatcgcatt tgccaactcc tcattggaaa
     4081 taaagatttt cataccaagg acaataaaag taccactact attcaaattt ggttccaaat
     4141 aatagaactc tgtacctaac cctttttgtt ccaatatttc cattgatatt tgccgttggc
     4201 gaaatctagc gtacttcttt tgaatattta acgcctcatt gcagactact ttcttacaaa
     4261 aactgtcaaa cgtatgttga atgtgggttt cctctgagtc gcccataata ttctccccct
     4321 caaaaaattc attcaactcg ttttacttct atccctttcc attattacta caatctagga
//
LOCUS       DAFGFZ010000165         4326 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000165 DAFGFZ010000000
VERSION     DAFGFZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.167"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..213
                     /locus_tag="LR726_002779"
     CDS             <1..213
                     /locus_tag="LR726_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217797.1"
                     /translation="NKWSRNDYLKLLVENDFDRPLMEYKQEKFDQLLEKFSEIQSYNT
                     KILEEYVSQNNRIIEILIEQNRGSEL"
     gene            215..637
                     /locus_tag="LR726_002780"
     CDS             215..637
                     /locus_tag="LR726_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217798.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            656..2248
                     /locus_tag="LR726_002781"
     CDS             656..2248
                     /locus_tag="LR726_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217799.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2269..2589
                     /locus_tag="LR726_002782"
                     /pseudo
     CDS             2269..2589
                     /locus_tag="LR726_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2750..3891)
                     /locus_tag="LR726_002783"
     CDS             complement(join(2750..3663,3663..3891))
                     /locus_tag="LR726_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL6217800.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            4139..>4326
                     /locus_tag="LR726_002784"
     CDS             4139..>4326
                     /locus_tag="LR726_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217801.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYP"
BASE COUNT         1509 a          717 c          874 g         1226 t
ORIGIN      
        1 aataaatgga gtcgtaatga ttatttaaag ctactcgttg aaaatgattt tgatcgtcct
       61 ttgatggaat acaaacaaga aaagtttgac cagttattag aaaagttttc agaaattcaa
      121 agttataaca caaagatatt agaagaatat gttagccaaa ataatcgtat tatagaaatc
      181 ttgattgagc aaaatagagg aagtgaactt tgatatggca aaggttatag acaaacacat
      241 ttactttcac caaacacata tcgatgtatt ggatgaaact atttctactc atgcagaggt
      301 taaaaactat agtgaagctg ttcgcttttt atgtatgaac tatgatccag aagtggagaa
      361 aaaagtaaat tccaatcaag caaagttgaa tggaatgagt aaagaaattt ctattttaac
      421 cgaaatggtt tcagaatttg cggatcttca tttgaaagat acaggaatat taattggtaa
      481 aaattcaact gtttatcaag aagcaaaaga acgagtggaa ggaaaaatta aatccaatca
      541 aacaaaaaaa tattcccctc agttgaagaa acctgtgaat gaatctgttc atgccagagt
      601 agatccattt gatccaacga agattctaaa aagataaaaa tgaaggagga taataatgac
      661 tcaatcgcct tcagttattc tcacttgtca atttactttg ccgaataaaa agtcattttc
      721 tacttatatt gactatatta caagggaaca ggctcttgaa gaaaaggaac gacgtacgcc
      781 cgaagaagaa caggaacttc aacgaatcaa gaatgcttta gaggagtttt atattcctga
      841 aggagaaact ttttcggaga gtaagacggg taaagaattc agtgaaacgt cgaatgaagc
      901 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
      961 ttatctttct tatatgacac gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
     1021 tgaacaaaaa gaataccaac gcttgaacca agcaatgaaa aaatatcaaa cagaacccca
     1081 taaaaaagct tcaggaaatc ttcctggagt tttttctata gcttcagacg aagtgaaagg
     1141 aagtgatctg aaggagatta agcaaatctt ccaaagagga caacaaaaag gctcaatcat
     1201 ttatcaagat gttgtttctc atgataattc gtatcttgaa aaaataggat tgtacgatcc
     1261 caaaacagat actttagatg aagaagggtt aaaagcagct ggaagaaaaa tgatggagac
     1321 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
     1381 cactaaacat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaacctta
     1441 cacggaggtt gaaaatggtg tgagttatat cgtaccgaaa ggtaagagaa gacaagcaac
     1501 attagacgca atgaagacca attatgtaca tgagttagaa cgtttttctt atgaaaagga
     1561 tcgaatgatt ggtatggaga aacgaaattt attgacgcgg aaaagtgatt tgcgtaacac
     1621 cttaacaaaa gtggtaaaag agccagcgcg ttacgatcga cacgcgatga acttattaaa
     1681 tgaagtatat aaaaccctac ctgacaaacg ttcggaatgg aattacgggg atgaaaaacg
     1741 aaccaaactt tcaccataca caagagagaa actcgatgag ttaaccaaat atgttttaac
     1801 aaaagaacct gaatatcaag aatatataca gatttcaaaa gcattgaaag aagaagcaaa
     1861 aagtctttat ggggaaagca agagagaatc aaaggatgca gagaaaaatg ctctctttga
     1921 tttaaaaaag cgcacaggaa atgccatcct aacagagctg aagagacagg atagtcaaat
     1981 aaaaccatta atagaaagtg ttgatcagat gcaatctatt tacaatgaac gatcctttcg
     2041 aacgcttaaa caattgccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg
     2101 caggaaacaa ttccaacaaa gagttgtcac gcagcggagt ttgcgtcaat taaatcgaac
     2161 gattaatgcc catgaagaaa aataccacgc aagtaaagca tacgaagagc aacaacgaag
     2221 aattcaaatg gaacaagaaa gacagtaaag acagtaggag gaatagcaat gaaacaatat
     2281 ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga acaacttcct
     2341 gattgtttag aaaaagagca tttgttccag ataagagaaa tgttgagaaa cgaacagttt
     2401 gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtattg ttgtaatcat
     2461 gtgaatggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc accttattat
     2521 actacttggg aaatgaatga agagggaatc aataactttc catgtaaaag cattggattt
     2581 gagtcaatac ctcggacacg atttgtgaga attttttcga gattccagcg ggtgagtatc
     2641 tcatcgtttc gaccagcggt caaaccaaaa ccgtttgacc gctgatcgaa gcgatgaagt
     2701 tgaaaccctg tggaaatctc gccaaaaaaa tgtaagtggt agctaggaac taagcttgca
     2761 atgccaactc ctccatctga tttggtgtta aatagttgat actactatgg attcgcttgc
     2821 gattgtaaaa tccttcaatg tattgaaata acgctaaata agcagcttca aagttttcat
     2881 acgcttttga tggatagact tcttcttttt tgagtgaggc gtgaaacgat tcgataccgg
     2941 cattatcgta gggacagccc ttacgactga aggagtgccg aatgttcaat tcattcagct
     3001 ttgcctcata ggcttcgcta gtgtactgag aacccaaatc tgagtggatg atcaagttct
     3061 cggagggtgt ttggtttaag agcgcattgt caagggtttc gataaccaat tctttcgtca
     3121 tctgcttgcc aaacttccaa ccgatgactt tctttgtgtg gaggtcttga atagtggaga
     3181 ggtaggtcca gccatctttt agggtatgaa tataggtaat atctgtcacc catttttgat
     3241 tgatcgacgt agtggtaaaa tcttggttta acaggttttt acgtccttca cttttaactt
     3301 tcgcttgttt gccaggcttg tacttcttga gggtgactga acgcaagcct aattttctca
     3361 tcagtatttg tactttcttt aaggacaagg tctcgccacg gccaacaaga atcttatgaa
     3421 ttttaggcgc accatagata cccttgtttt caaagtagat tcctgaaatc aagcggttta
     3481 agcgtttgtt ttctgattgg cgcttggaag gtttcttttt cttccagtca tagtagctgg
     3541 atttcggtgc ttccaaaacg gtacacattt gttttacgtc atgatcatga acatttgatt
     3601 gaataaattc gaagacatca ctcattttta cttttgagcg aatatggtta aagccttttt
     3661 taggatagtg ttttcctcct gcatgcgggc catttccttt ttcatcgcta ggatttctgc
     3721 ctcagtcatg ccggttgcta cattctttgt atgaagcttc ttccaacgat aaatcgtttg
     3781 tgtagcgata ccatattgcg ctgccagttc ttcgaccgat tggccagact ggtttaaatc
     3841 taagatcatc ttcttaaagt cttcatcaaa ttttttcttg gcttttgcca tcgtggacac
     3901 gtccttttct tagtagttag attctaccaa atcgtgtcca tgaaatcatc ctaacatcac
     3961 attgaagagt ccatttcact cacagagtgt tagttgttaa gtgaataaaa gaagtaaaag
     4021 agtcctgaac gtgattgtcc aggactcttt ttttcaatga attgttttcc gccaactcat
     4081 tgagtggtgc gatttgcgac tacattatat caaaagaaag agaagggagg aagcagaaat
     4141 ggttcattca acgattggag ttgttgttga aaaaagcaga gacaatctcg tttttgtgac
     4201 agaaattcaa acaggcagat cttttgtcgt cacagacaaa gcggcaaaag cttatcaaag
     4261 tagcgatatt ttaacgttaa atctgactac gaagacgttc gtcgatgcga cagaagatta
     4321 tccatt
//
LOCUS       DAFGFZ010000166         4237 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000166 DAFGFZ010000000
VERSION     DAFGFZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.168"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..652
                     /locus_tag="LR726_002785"
     CDS             <1..652
                     /locus_tag="LR726_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL6217802.1"
                     /translation="EKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLHS
                     SLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCLL
                     SIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPLY
                     HSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(715..1242)
                     /locus_tag="LR726_002786"
     CDS             complement(715..1242)
                     /locus_tag="LR726_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL6217803.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1262..2209)
                     /locus_tag="LR726_002787"
     CDS             complement(1262..2209)
                     /locus_tag="LR726_002787"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL6217804.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(2221..4107)
                     /locus_tag="LR726_002788"
     CDS             complement(2221..4107)
                     /locus_tag="LR726_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL6217805.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         1090 a          894 c          748 g         1505 t
ORIGIN      
        1 ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta atgcagtcac
       61 ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag taaaaggtgc
      121 ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga tgatgattct
      181 tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa aaaaggtgtt
      241 ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata ctcctttttg
      301 tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct ggttgtttgc
      361 gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag tatcaaaagt
      421 atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg ttcctcttta
      481 tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt cttacacgat
      541 aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt ggcatttgtt
      601 tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt aataaaaaaa
      661 ataaagctat ggatgggaca gctctaaata acagctagtc tatccatagc ttttttatat
      721 cgtcaattcg cctgtcccgc tttttacagg attctcatta cgctgatacg tttcaaattg
      781 atgcggataa cgattttttt catccatttc tccttgacta gtattgatca gtgaccaatc
      841 agaccagtca acaggtggaa aataagcatc tccatcgaat acatgatgga tgactgtacg
      901 gtataatatc gtacaaaatg gcagaaattc ttgataaaca gctgaccctc cagcgatgaa
      961 aaagataccg tctgtttctt tttccagagc aagcacgtct tccacactat gcatgacatg
     1021 cacgccttct gctttatagg aagaatctct cgtcaaaaca atcgtctgac gattcggcaa
     1081 cggacggcta cccatccctt cgaatgtttt tcgtcccatc actagtgtat ttgcttctgt
     1141 catttgtttg aagaatttca gatcatttgg caaacgccaa ggaagacggt cttcttttcc
     1201 aatcaatccg ttttcatcct gtgcccaaat tgcagctaac attttctcat cctttctgat
     1261 tctaaacagc gataggagct ttgatcgccg gatgtgggtc atatccttct aattggatat
     1321 cttccatctc aaaatcaaat actgactgct tttcctgatt caaaactaaa gtcggaaatg
     1381 cgcgtatttc tcggctcaat tgttctttca tttgttcgat atgattgata taaagatgag
     1441 catctcctaa tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt gcaattaggt
     1501 gagtcagcaa cgcataactt gcaatgttga acggaacgcc tagaaacaca tccccactgc
     1561 gttgatacaa ttggcagctt agccgtccct cttgaacata aaattgaaac atcgtatgac
     1621 aaggaggcaa agccatgcta ggaacatctt ccggattcca tgcagaaacg atcaatcttc
     1681 tagaatcagg agtctttttg attgcttcga tcacttgttt gatttgatcg ataaagccac
     1741 cgtcttttgt ttcccagtga cgccattgat agccgtagat gtgtccaaga tcgccgtatg
     1801 tttcagcaaa ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc tgctttttat
     1861 attcttcatt gaattcagga tctgtcaaac atctatggcc aaaatcagtc atatctggtc
     1921 cattatagtc tggacttttg atgtagcgtt caaatgccca ttcatcccaa atatggtttt
     1981 tattttgtaa tagatagcgg atattcgtat ctccttttaa aaaccaaagc aattcgcttt
     2041 tgatcaatcc aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt tgcagatcaa
     2101 accgcatttg gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct tctttacgat
     2161 gcccttcttc caacaatttt cttcctaagt caagatacgc ttgttccatc ctttttcctc
     2221 ctaatcttct acccattcac tcaagtattc ccaccgtgcc attttttctt caagttgtgc
     2281 ttctgtttca gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat ctccttgatg
     2341 attcatctct tcttggagca atgagatttt ttcttcaagt tctgcaatct catcttcaat
     2401 cgtttcccat tctttttgtt ccatgtaaga taatttcttc ttttcagcag gttccttgct
     2461 ctgtattttt ggtttcgctt tttcagcagg ctgctgggtc tctttttgct tcagtaaata
     2521 actggacatg tttccgtaat aaagttcttg ttttccgttt ccttggaata ctaataactt
     2581 atctgctacc ttgtccaaga aataacggtc atgggaaaca gtgatcacag cacctggaaa
     2641 acttctaaaa taatcttcca aaatcgtcaa tgttgctatg tccaaatcat tggtcggttc
     2701 atccaataat aaaacattag gttgctgaat cagcagtttc aacaaaaata accggcgttt
     2761 ttctccgcct gaaagtttgc ctatcagtgt cccatgcata aatcttggaa ataaaaatcg
     2821 ttcaagtaat tctgctactc cgatttgact gccatctgct gtttttgctt cttcagcagc
     2881 ttcttgtaag taagcaatca tccgtttatc tggtgccatt tcttcatttt cttgtgtata
     2941 atacgcaaga tgaaccgttt caccgataga cattgtaccg ctatccaggg gaattctgcc
     3001 agctagaata ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa tacctagtct
     3061 ttcccgagat tgaatcaata aatctaaatg ttctagcagt gtttggtcat ttatacggta
     3121 actgccatct ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta ggttcagctc
     3181 taaatcatct tcttggttta cttggtacaa gttctccttt agatcttcga aacggttgat
     3241 ccgtgcttgc tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag ctaattcctg
     3301 tttataaagc cgcttgcgct tttcttcttg ttctacttct acacgatcac gttctgcttt
     3361 ttctaagacg taagcttcat aatttccttt atactcgtat aattttccaa atgaaagttc
     3421 gaagattcgg tttgctactc tatctagaaa ataacgatcg tgggtaacca tcaatagtgc
     3481 gccacggtat tgtttcaaat agttttccaa ccactcgatc gcttcataat ctaaatggtt
     3541 tgttggttcg tctagtaata gtaaatcggg ttcatcaatc aacacttgcg ccagactaac
     3601 tcgttttttc tgaccacctg ataaagtact gattttttta tctaattcac tgattcctaa
     3661 tttttgcaaa ataatctttg cgttggtatc tgtagtccaa gcatcttctt tattcatttg
     3721 ttcttctgct ttagcataac gtttttgcac atcagggtct tctccgtttt cccctagttc
     3781 gatcaatgct tcctcatatg ctcgaacagt ttggatcaag gggctgtttc cttgaaagac
     3841 tgcttggaga acagttaatt catcggaaaa ttcagctgct tgagaaagat aaccaatacg
     3901 gtaatcagtt ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga taccggcaat
     3961 aatatttaag agacttgttt tacccgtccc gttcgtccca atcaacccga tacggtcttt
     4021 ttcgtggacc aaaaaagaaa tctggtcaaa caaatctttt tcaccataag tttttttgag
     4081 gtcagttact tttaattctt tcaacatgtt ccctccttat cttggatatt gtatcataaa
     4141 aatagtgaac ttcaggaaaa tttttttggt atttgttctt tttgtgttct caaaaaaaga
     4201 acaggaccga ttcgatcctg ttctgatatg ttttgtt
//
LOCUS       DAFGFZ010000167         4231 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000167 DAFGFZ010000000
VERSION     DAFGFZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.169"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            795..1562
                     /locus_tag="LR726_002789"
     CDS             795..1562
                     /locus_tag="LR726_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBL6217806.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            1588..2130
                     /locus_tag="LR726_002790"
     CDS             1588..2130
                     /locus_tag="LR726_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217807.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            2131..2697
                     /locus_tag="LR726_002791"
     CDS             2131..2697
                     /locus_tag="LR726_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217808.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            2712..2960
                     /locus_tag="LR726_002792"
     CDS             2712..2960
                     /locus_tag="LR726_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217809.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTENLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            2980..3219
                     /locus_tag="LR726_002793"
     CDS             2980..3219
                     /locus_tag="LR726_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217810.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            3590..3871
                     /locus_tag="LR726_002794"
     CDS             3590..3871
                     /locus_tag="LR726_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL6217811.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            3861..4202
                     /locus_tag="LR726_002795"
     CDS             3861..4202
                     /locus_tag="LR726_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL6217812.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT         1583 a          567 c          873 g         1208 t
ORIGIN      
        1 taatttttct attcaatcaa taaataatct atatttaaga agaaatatga tgatagaaac
       61 atctcctaaa gccaccgcaa gccgttctaa gcatagctta gcccgacatt gctagatatg
      121 ctcatctcac catgaacgtt tcctaaagcc accgcaagcc gtcctagtca ttgcctagtc
      181 cgacatcgct agattagtgc atctcgccat aaaagttttc taaagccacc gcaaaccgtc
      241 ctagccatcg cctagtccga ttttgcttga tcggttccat ttctgtttat ttagtgggtc
      301 aacaccgtaa tcttaaatag ccatcgccta tttaagatta ctaggtctgc tttgaaggta
      361 aaaagtcttt cattcttcaa gacttttagg ctttcaagct acgcctgaaa gcattgaaat
      421 tcatcgctct ttatttggtt ttgccattta taattcctta cgcccattat ctttttcgcc
      481 tgtcttgcct tgtcagaagt gtttagagct tatctcatct ttggattaat acaagtggcg
      541 taaatttttt tctaaacgca agcgttacga aaataattta cttaaacact tgtattaatc
      601 cggcagataa gaacgcattc taatcactcc cgacaaggca agacaggcga aaaagataaa
      661 taaatgaacg aaaggaagaa acataaaatg caaaaccaaa tagctatgaa gaaaaaaaca
      721 cctccctcta aaacaatcca aacaaaaaag aattttgaaa aaccaaaaat caaaaacaga
      781 aagaagcgtt caacatgaga gatagaattt tagaagttaa tcaagaaatg gtaaacaagt
      841 taacaaatat cagtgggaac tatgaaaagt acgatggtac tttacttgat aactatattt
      901 ttcacgatac acaaaatatc agaattggca gaagtaaacc gagagcgttc attctcatca
      961 gagaaaaata tttgaatgaa tggaatagca cctatgaaat ggttatgact gataactttg
     1021 aaaaggtatt ggatttcaga aatgctttgt tgggttctga tatgggaaat agcagtcttg
     1081 aatctattga agttgaaaac aagaccgaga atgaaggtga aggattgatt aagaatgtct
     1141 tgaaaatcga acaaaaatat atcagtgaag atagaagttt ttatagagtc acaagctcgg
     1201 ttggtctagc aaactttgct actaacttaa ttggaaacaa tgctagtgaa tctttgctag
     1261 tgatgtgtct gaatacaaag aatgaggtca ttgcttatag cgaagttttc aaaggtggaa
     1321 ttaatcaatc agtagcgata ccaagagaga tttttcaact agctttgcta aacaattcag
     1381 caaaaatcgc agtatcacat aatcatccga gcggatatga atttccaagt gaaaatgaca
     1441 aaagttttac tacgaatttg aaaaacgcag gaaaaacaat tggtattgaa ttgttagacc
     1501 atatcatcgt aacaagtaaa aacacaagct attactcatt tcaagaaaat ggttctttat
     1561 aaaaattaga gaaaagagga aaacaagatg ggcgaaagaa cacaagcgtt aattacagtc
     1621 aaatatggta acagcaaaac gaaagcaagc gtgtcgattc attatcagtg ggggtatggt
     1681 cgatatatgc tgatggacgt attgaatcaa ggaatttatt taatcaataa agcatacgac
     1741 ttacgagata aatcaagaga agaattagca aaaggaatct ttgatagaag tacaggggtt
     1801 atcaattttg ccacctatga atcaggtatg agtgcttatg atgaaacagg atttgatggg
     1861 cgctatgaac cttatcaaga aacgattgaa gatgtgatga agttttctga taacaatgat
     1921 ggctatgctc atttagagat ggtgttcgat gaaaatggat atagtctaga tgataaaacc
     1981 agattgcgat tgtttgatat tgagggtaaa cagataggac taaacaagtt ttgtaagaat
     2041 ggaaaagagt ttgctacaaa agattttatt aagggataca gattgttgct agaaaattat
     2101 ggaataaacc tagaagagag cgtgaaataa atgtttcaaa aaagaaaaga caaattccct
     2161 aatttagaag tgaaaagtca aaaagtgatt gatggtgaaa tgattgaact attccaaaaa
     2221 aattatggaa cgatagaaac taccgcaagt tgcaaatact actttcaagg aaagagaaac
     2281 acgtttaaag gctttgccga ttctgatgaa gaattggcaa agaaaatcat tgattggctt
     2341 gtcagcagtg aacacgtagc aagagaagat accgttagaa caacgattaa tggggaagtc
     2401 ctaacaggta ctagatgtgt ttgttgtaat catgaatttg tagaagatga aggttgctac
     2461 cgaatcaatg aatatggtga ttttatttgc cagagttgcc acgagataga atttccaacc
     2521 gaaagaagtt ggtttgaata catgctacaa gattatgaga tggaaaaaga aaatggtgaa
     2581 gtagtttttt gtaccaaaga ggaattgagt aagagaagcg atgatgaaat tgaagattgg
     2641 tgtgtgtggt tagtaggggc agatgattgc gttgcatatt acacaacagc agaataggag
     2701 tgtgaatcag catggaagga atggattttc tagatcatga agatttggtt gattttggtt
     2761 atacatggaa aggaatggta ggtattagca gaagtcttgc taatgccttt tacgaaagaa
     2821 attatgcagt atatgttctt tatgatgacg atacagaaaa ccttgttgac gaggaatata
     2881 aattggattt agagaatgtt ttatatggaa tagaaaagga agatttagca aaatatattt
     2941 tttcatggtt gggtcaataa gtgaaaggaa gatagaaaca tgacaaaatt taaaagtaag
     3001 ggtatgcaat ggtgttctta ttattatgtt ggaaatgatg aagatttatt gagaaaaacc
     3061 caacgaaaag gttatccaga taagatattg aaacaaatag gttattacat gccagaagag
     3121 aggatagaaa aagaattttg tgaggttctt tttgatagtg aacagctatt agttttgtta
     3181 acccaaaata aattgatttg ccaatttgaa aataattaag atgaggtggt gaaccatgaa
     3241 tgaagataag atggtattaa gtgaatatgc tagattgaga actattaatt ctttagcatc
     3301 tcatttaagt tgttctttcc ttacaaaggt gctaaaatta atttacaaat gtgaagattt
     3361 tgtacaagag aagcacaaag acaagcctac tgcgaatagg cttgtctttt tttcattcat
     3421 tacgattccg tatgtgtggg ttagcgacaa tttgttcaat taccgtatga tatagcatct
     3481 taatttgatt agattacttg caatgtagtt tattaagagt ataatataat cataaatgtg
     3541 gataatttga gaaataatga gtgttttata ctcaaaagga gtggatgaaa tgaaggtcaa
     3601 aaatccaact gaaaaaagca gaatccaagt cggaatagac aagaatttaa aagagaacgc
     3661 agaaatgata ttggaagagc taggtttaaa tcctacaaca gcaataacaa ttctttacaa
     3721 acaagtagtc gctagaggtg agtttccagt agagataaag ctaagtgaag aggagaagca
     3781 aggaatcaga ttgcaacagc taactaagga catgccagta gatgttctag atacagatga
     3841 aaagttagaa gagtggttcg atgaagccta atgagatagt aacggtctat gttgcattta
     3901 cagataaaaa tggtggtaaa agaagaccta ttttggtggt tagtgataaa gaagatagag
     3961 tagaattttt tggaatcaca agtcaatatg agaagaaatc agaccaaata aagagagtgt
     4021 attttcctat tggagaatgg gaaaaagcag gtcttaaaaa gcaatcatgg attgacgtag
     4081 ggtctttaaa ggctattccc aaaagccgag agaacatatc atttaaaaag gttggtatgc
     4141 tatcagctag tgatacgaaa aagctaaata agtttatcga gagattacaa agaaaagtct
     4201 aacaaggctt ttcttttttt gcttatatgt a
//
LOCUS       DAFGFZ010000168         4220 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000168 DAFGFZ010000000
VERSION     DAFGFZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.170"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            25..264
                     /locus_tag="LR726_002796"
     CDS             25..264
                     /locus_tag="LR726_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217813.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(626..886)
                     /locus_tag="LR726_002797"
     CDS             complement(626..886)
                     /locus_tag="LR726_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217814.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1071..1568)
                     /locus_tag="LR726_002798"
     CDS             complement(1071..1568)
                     /locus_tag="LR726_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL6217815.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1698..2408)
                     /gene="budA"
                     /locus_tag="LR726_002799"
     CDS             complement(1698..2408)
                     /gene="budA"
                     /locus_tag="LR726_002799"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL6217816.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2421..4085)
                     /gene="alsS"
                     /locus_tag="LR726_002800"
     CDS             complement(2421..4085)
                     /gene="alsS"
                     /locus_tag="LR726_002800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL6217817.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1213 a          899 c          662 g         1446 t
ORIGIN      
        1 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
       61 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      121 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      181 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      241 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
      301 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
      361 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
      421 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
      481 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
      541 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
      601 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
      661 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
      721 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
      781 caacacaatt ttctaattta ctgattactt ctccaaccac tttttgtttc aagccaatag
      841 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
      901 taatgttttt ccattattta aaatgataat accattaaaa tatgattatg aaaatcaata
      961 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1021 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
     1081 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
     1141 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
     1201 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
     1261 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
     1321 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
     1381 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
     1441 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
     1501 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
     1561 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
     1621 tgtaaacgct aggtttcctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
     1681 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
     1741 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
     1801 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
     1861 tgtgtatcat cgataaaatg caagtgaaat cccgctgcag caactccttg gaataattca
     1921 ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc tggttgagac
     1981 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     2041 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     2101 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     2161 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     2221 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     2281 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     2341 gttccatcca tcaagccagc catcaaagct cctaatgtcc cgtgctggta taagattttt
     2401 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     2461 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     2521 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     2581 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     2641 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     2701 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     2761 tcgccggaaa ctgatacgat ttgtgtattt ggacgcacca atgcagcaga aatcgcccat
     2821 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     2881 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     2941 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     3001 tctccttttg attgaacaat gtcccgctcc gttaattctg cttgaagaga tgctaaatat
     3061 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     3121 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     3181 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     3241 tcgattggat catagccaat agcaataact agatcacttc gtttcaaaag catatctcct
     3301 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     3361 actccagctg cttggaatgt ctctacaaca ggcaatgctg tctttccaac taattgcctg
     3421 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     3481 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     3541 ggatcgctca atggcttgat gacattccct tggacaggtg catccactac atcttgcggg
     3601 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     3661 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     3721 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     3781 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     3841 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     3901 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     3961 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     4021 tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt cattttttcg
     4081 tccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa
     4141 ataattgata cagtttctaa acctgtttca ttcaaaagta tagcacgttt tatttcattt
     4201 gtttagccat atgtttcaaa
//
LOCUS       DAFGFZ010000169         4178 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000169 DAFGFZ010000000
VERSION     DAFGFZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.171"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            443..1411
                     /locus_tag="LR726_002801"
     CDS             443..1411
                     /locus_tag="LR726_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL6217818.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1422..2237
                     /locus_tag="LR726_002802"
     CDS             1422..2237
                     /locus_tag="LR726_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBL6217819.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2305..3015
                     /locus_tag="LR726_002803"
     CDS             2305..3015
                     /locus_tag="LR726_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL6217820.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3051..>4178
                     /locus_tag="LR726_002804"
     CDS             3051..>4178
                     /locus_tag="LR726_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBL6217821.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1396 a          628 c          890 g         1264 t
ORIGIN      
        1 tcagttttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
       61 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
      121 ccagtagttt cctaattcaa ctgtgtgcaa aaacgatttc ttctaaacaa atcaagacaa
      181 acttataaac cttcatccca gcaccataat tatttaaatc ataggcattt ttcgaaatta
      241 gtatatgtac tcaagtttca ctgcagatta tttatatttt ttagagaaat aaatttttcc
      301 tattgacaaa cacaaagaaa gcggatacaa tgcaattatg tcacatgacg ttatatgacg
      361 ttatgtgata tctttacatt taatcagtta cttttatttt ggataaaatt aacattttta
      421 aaattttaag gaggaaatgt tgatggatgt agcaaaaacg gttaacgcaa ttgtagaaaa
      481 aaatgatgga gagatttctg gaatttattt ggtggcttgt ggcggttcgc tggtagatat
      541 gtatgtctca gattattttc ttaaagccga agcatcgaag tgtattacag gcttatatac
      601 agcgaacgaa tttaaccatg taaccccaaa gcaattagat gataaatctg ttgtcattct
      661 atgttctcat ggaggaaata cagcagaaac ggtaaaagca ggagaagtcg ctcaagctag
      721 aggagctgtg actgttggtc tgactcataa taaaactgcg gatttattaa aggtttctga
      781 tcatagtttc ctttacgaat ggggagatga tgcaaaaata gtcaataatc ctatggccat
      841 cattcttgat ttaactgtat ctttggtcaa tgttaccgaa acatttgaac attacggtaa
      901 attcagagaa ggcatgaagg ttatcgatac cgtaattgaa aatggcatta agcaggtgca
      961 ggatcgtatt aaagtgtttg ctgataagta caaagatgaa acaatgtttt atatcttagg
     1021 tagtggacca tcatatggac atgcttatgg tttttcaatt tgctctttga tggaaatgca
     1081 atggcttgat tcaacttctg ttcattctgg agaattcttc catggacctt ttgaagtaac
     1141 agacaaagat acgaatttta ttctcttgat gagcattgga cgcactcgtg ctttagatga
     1201 acgggcaaaa cgattccttg ataaatatgc agaaagagta gaaatcgttg atgcaaagga
     1261 actaggattg gatatgatat cagatgaagt ttctgaattc ttcaatccca ttttattcta
     1321 cagtattctt tcagaatatc gttcagcttt agcagatgta cgcaatcatg atttagatgt
     1381 tcgtcgctat atggggaaag tggagtacta ggaggattaa aatgaaggta ttaggcttag
     1441 gtgataacgt cgtcgataaa tatgaaaatc tgggaattat gtatcctgga ggaaattcat
     1501 tgaattttgc agtttttgca aaaaaactgg gacatgaaag tagctttatg ggagtttttg
     1561 gctcagatga ggaggcagct catgttttat cagcaataaa agaaatagga ctggacaaca
     1621 gtcactctcg ctttgaaaaa ggcgagaatg gctgtgccag agtgaagatt gatgagggcg
     1681 accggatttt ccttggcagc aattcgggag gtgttactcg tgaatacccg attcagctta
     1741 ctgaagaaga cagagaatat ttaaaccagt ttgaattgat tcatatggga ttatatagcc
     1801 atgtcaatca tctattagaa gagttacaaa atgtttctgg aaagatttct tatgattttt
     1861 ctgacgactt tacggaagaa gaggttcagc gggcaattga taaagttgat tttggcttct
     1921 tttctataga agacttttcc gatgaagaag taaagcagtt tttgatgaag catttttgtg
     1981 agcgaaatga tgtattgatt gcaacccgag gagaaaaaac ggctattggt tatgatggag
     2041 caaagtttta tgaagtgaac cccgtgctag aagaaccagt tgatacaatg gcagcgggag
     2101 attctttctt aacagctttc ctactttatt ttctcgaagg ggacgatata gtcaccgcga
     2161 tgaaaaaggg gaatgaattt gctgggaagt cttgtctggt tgacggttcg ttcggctttg
     2221 gtattcatta cgattgagct tgttttaaat tcctgcattt gataacctaa agaaagaaat
     2281 cccggtaaag tggaggttaa aaaaatgctg gatacaaatg cacaattacc attgtactta
     2341 caattaaaag aaatattaaa aaataaaatc aatacagggg agtatcccga gaatacgaaa
     2401 attcccgctg aatcagagct gatagaaagt tatgaggtca gccgaatcac agtgagaaaa
     2461 gcagtagaag aactggtaga agaagggtac ctacttaaaa aacaagggaa aggaactttt
     2521 gtcaaccggc ataaagtttt tcgtaaaatt gaatatatta tgggtttcag tgagagttgt
     2581 aaatctaatg gttttacacc gactagcatt gttttggaac gcaagatcat tccagcaact
     2641 gaagggatgg caaaaaaact cgatatcgct caaggtgatg atgttttata tatccagaga
     2701 aagcgaatgg cggataacat ccccattatg ttagaaaaca attatttttc ttatgaaaga
     2761 tatagtttct taatggagga agagctgact gattctttgt atgaagtact taaagtccaa
     2821 ggtaccttag ccatcaatcc tggggaaact gcacttgaaa ttgtcacagc tgatgaccaa
     2881 attgccaaaa taatggaagt agctgttggg acgccctttt tctttatgga tactttaatc
     2941 aacgatcaaa acaacatacc aattcatgta ggtaatcaat attacctggg agaagtatat
     3001 cgtttttact tatgatttcc tgtttggatt tcaaggagta gattttattt atgaagcaaa
     3061 tattgcggtc aaaaatgatt tttgatggag agcagccacc tttcaaaggt tatattgtaa
     3121 tcaatgaagg gatcattgaa aaggtggaaa acaactggaa ttacagtgat ctattaaatg
     3181 aaaacacgga attaattgct tatgatgatc aattcgttat gccagcaatt catgataatc
     3241 atgtattttt cagtggttat ttggctatga atgctggcat agatttaagc gatacatctt
     3301 cagctaacga ggctgtagag cgtattaaag ctgtaatgaa ccataaaaaa gcagatgaac
     3361 caatttatgc tcatggctgg gatgctgccc tgtggaaaac tgacccatca gaagagctat
     3421 tgaatgaaat aaaccacttt ggtccaatca cagcgatcga caaaaatcgc agccattgtt
     3481 ggatgaatgc tattgctaag cagacttatg gttttacaga aaaagaaacc agtgctgaaa
     3541 gtcgagtatt attaattaag gaaatgctag caaacaaaaa gcttttagca gaggtttacg
     3601 ctgaatttga agcattactg ctttcaaaag gtgttatatc aatgaaagaa atcgtttttg
     3661 atgacgcaga atttctttct aatatacctc agaggaaaat acttagtaac ctatatgtgc
     3721 aggcagtcca agaacctcta cagcatgaac ttctatatga gtataaggaa aaagttttcc
     3781 ctgaaaaggt ccgctttggt ggagtgaaga tcatggtaga tggtgttgtt gcggatagaa
     3841 gtggagatat ttacggtgtt tatcagtcag gagtagcttc acccgagatt gactatgagg
     3901 caattaaagc agaagttgct gcattcaatg aagtagagat tccttgctgt ttgacaacgg
     3961 aaggagataa agcgggttat aaagctgcgt caattcttgt cgaatttgga aagcggttac
     4021 ctgaaggcgt ttttaattcg attagtgatt tggaaatggt tactcaaaag acagcagaaa
     4081 aaatgaacga aggacaagta gtagcagagg tatatccaca aattctcggc ttgaatcaaa
     4141 cgttagaaga ggcttacatg tcaactgtta taaaaaat
//
LOCUS       DAFGFZ010000170         4170 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000170 DAFGFZ010000000
VERSION     DAFGFZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.172"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            430..597
                     /locus_tag="LR726_002805"
     CDS             430..597
                     /locus_tag="LR726_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217822.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            671..1897
                     /locus_tag="LR726_002806"
     CDS             671..1897
                     /locus_tag="LR726_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6217823.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2055..2672)
                     /locus_tag="LR726_002807"
     CDS             complement(2055..2672)
                     /locus_tag="LR726_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217824.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            2978..3316
                     /locus_tag="LR726_002808"
     CDS             2978..3316
                     /locus_tag="LR726_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217825.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            3408..4113
                     /locus_tag="LR726_002809"
                     /pseudo
     CDS             3408..4113
                     /locus_tag="LR726_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         1487 a          681 c          693 g         1309 t
ORIGIN      
        1 ttaaattttt acatacctgt ttatgaaaaa ctaaataatc tgtcaaaaaa atatgattgt
       61 tcatttctaa atatgttttc caattttaaa atctttttta gcatttttgt ttccttttca
      121 tatggttttt cgttgttaat agtgactagg atgatattcc tattttgacc attgacaatt
      181 aaatataatt tttgaataac gttccctgct attcgagcta agaattcaag tacgccatga
      241 cctatttagc gataactact tagagatttc tttttgtttt gggaagacaa aatgccatga
      301 caatagcagt agataatgat acttccgtaa ggctcgccgt aaactgggga gaggtgaaat
      361 tcctatgtgc ttacccaagt caattcaaaa attttagttc atttataagg ggatgatttt
      421 attaaatcaa tagtctatct aaacaaaaaa caaacctgtg agtttttagg aattgataac
      481 aatactttag ataaatggat tcgtatatat aattttcccg taataaaaat agacggtata
      541 attcgctttg atgtagaaga tatcaaaggc tttatggata cgttcaaaaa gatttgaatt
      601 ttacttttga ctgcatatac taaaagtagc gaaatatact gccttacttt tttattaagg
      661 agtgaggaag ttgcctacga agctttcaaa tggaaaatat aaaactaatt tgagatatcc
      721 aaaaaaattt aaagaaatta caggtatcag ttcagaaaaa ttccaaaaaa catttccaac
      781 taggcaattg gctattaaag ccgaaaatga aatgaaaaag aaaatcgcaa aagtcttaag
      841 agaggagaat gcaaattcat tagagctcaa aggtcaaata aaattcaagg agttttataa
      901 atctaaatgg cttcctagat atgagttagg gcaaactact agatcccata aagctccaag
      961 cgatataacc atcattaata caaagaattt atttcgattg catattctgc ctatatttgg
     1021 tgagtatgcc atgagctatc ttaatgcaaa taccgaaata atatcagatg aactcacaaa
     1081 aaagtcgaag gaatatgcaa acataaaaat cattaaatgc tatgttcgtt ccatgtttga
     1141 tattgcagag gttctaaatt atattgaatt taatcgtaca acaaaaataa ttcaaagtat
     1201 tactgcacct aagaaagttg ctctggaaga aaaaagaata cgagaaggaa aacaagcttt
     1261 atcttcgaaa gaactcactg attggttaga cgctgtaaga gatgatttca atgatcaatt
     1321 actttccttt cacgattaca ctctttttat gcttacttta tatcttgggg atagaaagag
     1381 tgagacctat gctttgcaat ggaaatacat tgattttgaa aaccaaacag tccgtcttaa
     1441 atatgccttg gataaacaca caaaaaagaa atttacaaaa ggtcgaaaag atactattat
     1501 ccaagttccg gaaattgtaa tgacactatt gcaagaatgg aaatcagtgc aagctgaaca
     1561 actattgaag ttgaaaatta aacagacacc tgaccaatat ctttttagtt actccaaacc
     1621 ttctggtgaa gtaaattgtc cagtccatac tgattactta aattatcgaa ttaattctat
     1681 aaaaaaaaga catccccatt tggttcattt aactcctcat aaacttcgac atacttacgc
     1741 aactttagct agacaaggtg gggctgatat gaatcaaatt tctaacgctt tgacacattc
     1801 aggtatttca actacaaaaa tttatgtgaa tacacctgat ttagttgata aagctgtgtt
     1861 cgaagcgttt cagcgaggat tacaaaaatc taattaatgt gttctagaat gtgttctaaa
     1921 tataaaaaaa agacactttc caaataattg gaaagcgtct tgaacgttga tatataaagg
     1981 atattaacgt tttgagaatt gagatgcttt acgagctttc ttaagacctg gtttcttgca
     2041 aaattatatt agcctcaact gaaagattta tttttttgct ccctaacttc attagtaata
     2101 ccatgtttca ttcgatatct tctaaacgtt ctacgattaa tccctgttag ctcttcaact
     2161 tctttatctg tagatccagt caaaaacaaa ttaaatgcta attgaagtcg cggatcattc
     2221 tcacgatatt ttagcgtacc tcccttgtat ttcccttttg ctttagcgag tgcaattcct
     2281 tgttgttgcg tttcgcgaat ataccgacgt tcttcttcgg cctggtactt gtatagttcg
     2341 ataattaagt tattaagtaa tcgacgtaaa ttatcatcag tgatacctgt taacgctggc
     2401 aaattcaaaa cttccagtgt agctttcttc agctgaatta aattcatcgt ttctgttagc
     2461 tctttattat ttcgtccaag tcgttttaat tctgtcacaa cgacaatatc accttcacgt
     2521 atgaatttca tcatttcttg aaatccttct cgattggtat ctttcccact tgctttatca
     2581 ctaaaaattt tatcacactc tgataataat tctaattgtc ggtctaaatt ttgatctaag
     2641 ctgcttactc gagcataccc aattttcgcc ataataaaac tcctttaaaa ttctgaaatc
     2701 cggatcattt ttaaattctg ttgcccaact ctgttgtcat ttttctagat aaatggcata
     2761 aattttcagc gctaagtgac aaaagcaact aatttttcat gatagatttt taaatatctc
     2821 aatctatctc caaaattagt tgcttttgta gctatagttg actatgaata tgacaaataa
     2881 ttagaaaaaa ctgtgccaat taaatatcaa aatgacaaat tgatgaatca aattcaaatt
     2941 aaggggggca accctggaag tcttaaattt accctcaatg aatggtattg aagatgaaaa
     3001 tttaaggcgt ttgattaata gccttgtcat tgaattgtac aagtatcaag cagaatcaga
     3061 acgaaaaaaa attaaggaac gtcaggcaca aggaatcgaa attgctaaga aaaaaggcaa
     3121 attcaaaggt cggcaactga aatttaaaaa aaatgatcca cgtttaaagc atgctttcga
     3181 tttgtttttg aatggtttat ccgataaaga agttgaagaa caaactggga tcaatcgccg
     3241 aacgtttaga aggtatcgag caagatataa cgtgacagta gatcaaagaa aaaacaatga
     3301 aaagagggat agttaatgag tacggttatt ttagctgaaa aacaagccag gcattagcct
     3361 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     3421 tatttacaga tgaaacgttt atcacctttg attttgggca tttagtggaa ttagcagaac
     3481 caggtcatta tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg
     3541 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac
     3601 ttctcaaaaa ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata
     3661 tcgcctggtc gattatccat aaagcaaatg ccttttcaaa agataaaaca tttaaaagac
     3721 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     3781 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     3841 tcggcatgaa cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta
     3901 cattttcact aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag
     3961 ctagtataaa agtaaaacaa gggtcattta agggcgttct aagccccaca cagcgtttta
     4021 aaacccaaga ggagcttttt tgcttttgtt tcttctaaac aagctaaaat aggcaatcaa
     4081 gaggggataa ttgctgatgt tcaaaccaaa gagatggata ggctacaatt aactagacag
     4141 aaaaattaag gtgtgtagac tagaagaaaa
//
LOCUS       DAFGFZ010000171         4131 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000171 DAFGFZ010000000
VERSION     DAFGFZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.173"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            47..1348
                     /locus_tag="LR726_002810"
     CDS             47..1348
                     /locus_tag="LR726_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL6217826.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="LR726_002811"
     CDS             1375..1635
                     /locus_tag="LR726_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL6217827.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="LR726_002812"
     CDS             complement(2634..3869)
                     /locus_tag="LR726_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL6217828.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1188 a          666 c          862 g         1415 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa t
//
LOCUS       DAFGFZ010000172         4031 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000172 DAFGFZ010000000
VERSION     DAFGFZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.174"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            823..1164
                     /locus_tag="LR726_002813"
     CDS             823..1164
                     /locus_tag="LR726_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217829.1"
                     /translation="MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDR
                     WEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVN
                     KGKRYGIEVRS"
     gene            2089..3414
                     /locus_tag="LR726_002814"
     CDS             2089..3414
                     /locus_tag="LR726_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL6217830.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            3407..3754
                     /locus_tag="LR726_002815"
     CDS             3407..3754
                     /locus_tag="LR726_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217831.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT         1566 a          518 c          779 g         1168 t
ORIGIN      
        1 aaaaatatag aagtatatgt tgatggatca caagaagaaa gggattcctt ttttgatttt
       61 ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc tggtatataa acagctaatg
      121 tttgaagagt tgataaaaga tgcaattaaa gaaatggatc aaggttatgc tgagagagaa
      181 gaaagtattc aaaatcggat tgaaaagtta gcatatactg atccaataga tcaaaaaaaa
      241 cttgataaag caaaagctga attatctaaa ttgagaaatg aaaaaaataa agaagcgaga
      301 gaaatattaa cgcaagcgat agggttgaag agtcaaacga gaacacgaga tgtagcaaaa
      361 aaattagact ttgactttgg agattttgtt gattcagcaa atatgaaagc tagtaaggat
      421 tttggcaatg attttattgg aattgttact ggtcaaagaa cacctagaat ctatagaaat
      481 aaaagtaaat acggattgtt accgttcaga ggtcgggata ttaagattca caaagctgac
      541 aagttttata tctctttacc taaaggattt agacttaaag taaatttagg tagcaagcca
      601 agaaaagcaa gagatgttgc ttcagtttta caacacatta tggatggcga ttacaaatta
      661 aatcaaagta atcttatttt tgatggaaga aaattatttt ttaatctaac aatttctttt
      721 gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg aattgatcgt aaactttgca
      781 gatttgtgta gcggtgaatg tttaattaat gataaatctg taatgtcttt tggtgatagc
      841 caatatataa gaaacttcaa aagtaaaatt ttagcaatga aatcaaaaga acaaagccga
      901 ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat tagatcaaga tcgatgggaa
      961 gcaataaaga aaagagaaag taactttgtt aaaacttata ataatcaatg ttctgcaaaa
     1021 atcgttaaaa ctgctattcg caataaagct caaattattc gtatcattga accagataag
     1081 gcttatggcg aatgggctta ctatcaattg aaagaacaac tagtaaacaa aggaaaaaga
     1141 tatgggatag aagtcagaag ttagtaattt aaacaatcgt tgatttggca acgaaatagt
     1201 gagggaaaat ccactcactg aagggtaaac tccgtcaagt tgtgtgacca gacggaagga
     1261 cgaatgttgt tgaggtttta gaactatata aaacagcatg tttctcaaat tatgagatgt
     1321 taagaaaaat gattcgagag gttttagaac tatataaaac agcatgtttc tcaaacaaag
     1381 tgcagaacga aaatcaattc atcacagttt tagaactata taaaacagca tggttttaaa
     1441 acaagaaata agtcagtttt ccttttgctt tagtaaggaa aaatagagta ttctcaataa
     1501 aacttaaaat aagaaagaat aagcaaacct ttgttttaat agctaatcga agtaaaaccc
     1561 aaagccatat gaactcctga atcagcgaaa gtttatgatg attgggctta ctaataattt
     1621 aggaattaga tggtaaatct aaattaaaca atcgttgatt tggcaacgaa atagtgaggg
     1681 aaaatccact cactgaaggg taaactccgt caagttgtgt gaccagacgg aaggacgaat
     1741 gttgttgagg tttagaacca cataaaacag catgtttctc aaattgcctt agtggttgaa
     1801 ttgagatcta tcacgtttta gaaccatata aaacaacata gttctcaaat tgtttaaaat
     1861 gattgtcaag ttaaaaaaat ctgggatcat ataaaatagt atagttctca aatatgatta
     1921 gaaaccctat taaagagcaa tattaaacca acttacttaa atcgtttaat taagaacagt
     1981 aatctctttt agtcagactt ttagtctgac ttttttgtat ttccaaaatt tattctttac
     2041 aaaagcgaac gtacgtttgt ataattgctt gtgaatgggg gaataaaaat gtttgattac
     2101 agtagagaac cggtgaatga ctatttttta atcgacatga agtctttcta tgctagcgtg
     2161 gaatgtattg aacgaaattt agacccaatg actacaccgt taattgtcat gagtagatcg
     2221 gataacactg gttcaggtct gactctagct tcttcaccta ctgcaaagaa aaaatatcat
     2281 atcactaatg ttagtagagc taatgattta ccttatccat taccaaaaga actttatatt
     2341 gttccacctc gaatgaactt atatataaaa aaaaatatgg aagttaatca gatttttaga
     2401 aagtatgtcg cagatgaaga tttattgatt tattcgattg atgaatcaat tttaaaagtg
     2461 acaaaatcct taaatttatt tactagttca gaaacaagaa gcgagaggag aagagaatta
     2521 gctaaaataa tcaaagatga gattgagaaa gaaacaggac taatagcaac agtcggaata
     2581 ggagacaacc cattattagc taaactcgca ctggacaatg aagcgaagag tagtaaagaa
     2641 tttatagctg agtggactta tgagactgta cctgaaaaaa tatggaaaat aaacgagatg
     2701 actgactttt ggggcattgg atcacgcatg aaaaaaagat tgaatcatat gggaattatg
     2761 agtatagaag atttagcaaa ttggaatccc tacacaatta aaagtcagtt aggtgtaatc
     2821 ggacttcagt tatatttcca tgccaatgga attgatcgta cggatattgc tattccacca
     2881 gaaccaacca aagaaaagtc ttatgggaat tcacaagttc taccaaaaga ttatacgagg
     2941 agagaagaag tagaactagt agtcaaggag atggcagagc aagtagccat tcgaattaga
     3001 caacataatt gcaaaactgg ttgtgtacgc ttagcaattg gtacgtcaat tttggaagaa
     3061 aaatcaggtt tctcccacca gatgaaaata ccaattacag ataatacgaa agaattacaa
     3121 aattattgtc tttttttatt caataagttt tatgaggggc aggaagtaag acatatagga
     3181 gtaacttata gcagattagt atacacagat tccctacaat tagatttatt tagtgatcca
     3241 agaaaaaaga ttgctgaaga aaatttggat aggattattg ataagattag acaaaaatat
     3301 ggatttacag ctattataca tgcttcatct atgttagaag gtgcaagaag tattacgaga
     3361 agcactttaa taggaggaca cgcaggagga ttaggaggtt tgcataatga ttgaatataa
     3421 cgatagagga cgtttaaaat ggtcgggatt ttatttgtct gatcatactg aaagaatcaa
     3481 tgcagacaat gtacaacgtg ctaatcataa tttggcaaaa gaacaaatga cgactgaaga
     3541 gattgcgaaa atactgagaa ctgctgtcat aaaaaataga cccgtctgca ttcaaaaaga
     3601 ggaacgaaac gctgaaggtt actatccaga tgatgtagta ggaaaaatta ttggatatga
     3661 cgagcttggt gtatttgtgg aagaagaaaa agttcactat gatgaaatta ggaatgtttc
     3721 tttcgcggaa ttattaaaat ggagtcagat atgaacatta ttaaaaaata cgaattcggt
     3781 tatattacgt atgaacagct aatagaggaa ttatggggat atggtcaaca attaattaat
     3841 gaagtgggga ttgattgctt ttgtttctat attgaatccg gagcaggata ccataagaac
     3901 agatactata tatttccata tccaccaaaa tagtctctta gagattttta aattttctcc
     3961 aaatactttc aattattgtg aaatatttcc ctttaacaga attaggcaga agtcgcccat
     4021 cctctatagg t
//
LOCUS       DAFGFZ010000173         4008 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000173 DAFGFZ010000000
VERSION     DAFGFZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.175"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..1256)
                     /locus_tag="LR726_002816"
     CDS             complement(<1..1256)
                     /locus_tag="LR726_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL6217832.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="LR726_002817"
     CDS             complement(1270..2487)
                     /locus_tag="LR726_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL6217833.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>4008)
                     /locus_tag="LR726_002818"
     CDS             complement(2477..>4008)
                     /locus_tag="LR726_002818"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL6217834.1"
                     /translation="SEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQTML
                     YRELLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLAS
                     TYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLI
                     SQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYL
                     THPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAV
                     VMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPH
                     GVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFID
                     ASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVD
                     TFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHE
                     Q"
BASE COUNT         1110 a          860 c          674 g         1364 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacg
//
LOCUS       DAFGFZ010000174         3935 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000174 DAFGFZ010000000
VERSION     DAFGFZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.177"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(144..1055)
                     /locus_tag="LR726_002819"
     CDS             complement(144..1055)
                     /locus_tag="LR726_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL6217835.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1512..1940)
                     /locus_tag="LR726_002820"
     CDS             complement(1512..1940)
                     /locus_tag="LR726_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217836.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1968..2606)
                     /locus_tag="LR726_002821"
     CDS             complement(1968..2606)
                     /locus_tag="LR726_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6217837.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2610..3380)
                     /locus_tag="LR726_002822"
     CDS             complement(2610..3380)
                     /locus_tag="LR726_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL6217838.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1205 a          736 c          577 g         1417 t
ORIGIN      
        1 ctggattcta agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt agtaattaaa
       61 ctactgttga tttagttcta ataaaaagat ttagatgaac agttttattg atttaaatag
      121 ttcatatttt ttatttatac cgcttattta ctacttgctg tactagaatc tgaagtgaat
      181 gtactcaaaa cattttcaaa gtctttgtct ttgattttta cattagcgtc tttcaatact
      241 tcgccaatta ctttattctg gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct
      301 ttcaattctt ttttgtattt atccatgtcg ttgcctttgt tttggttttt caccatttta
      361 accacataat attccgtagt ataagttgaa gcattggtag aagtgatcac atcagaaact
      421 tgaccatctt tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg
      481 gaatcaaatt tcacttttcc gccatcttct tttgtcgtag tatcagttga cttgtctttg
      541 gctaatttag agaaatcgtc tcccttatca gcagctttct tcacatcttt tgcatcatct
      601 tcacttgcta cttggataat ttgagcttcc acttctggat ggaaagattc ccaagccgtt
      661 tttaaatctt catcggtcaa tttgatatgt ttctttagac cttcttgaaa ggctagactt
      721 tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca
      781 aatgaatcac ccaattgttt ctttgtttga tcatattgag aatcgatttc tttggttgat
      841 acgtcatctc catatttatc ttcaaatact tgataaacga tcaactgttg cacagcggaa
      901 tctgctcctt gcattttttt caagctatcc attacttctt gttcgctgat tgtaccgcct
      961 ttataactaa cggctgtttt ttcaccggca ttcgtacagc ctgacaataa gccagtgcct
     1021 agtacgatgg caagtgctaa aaatcttttt ttcatttatc aattctccta cttttcgttt
     1081 tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat catcaagaac acctttacta
     1141 atctgctcga tacgtcctga atagatataa ggctacacaa tccagaaagt tcgctcagca
     1201 gaataaatgt caagtagcac atcacacctt aatatactac ttcttttgtt accgtttttc
     1261 aagtctatct tgcgttccgt atttaacgta actaattctt agaacaaatg ttatcgattt
     1321 tgtttttctt cttccgctgc tgggatattc aaatcagaca cttttatgtt cgattttcca
     1381 gacatttttt tgttgttttt attatgatat atttttatac aactaaaaat gaatatatta
     1441 aaactaccaa aaataacttt taacgctgtt tcattatatg ttttttctat tttatataaa
     1501 cttttggata cttaatacca accggttgga ttgtaccaac atgagaatgt gtcagttcta
     1561 tttcctgtcg cacttgcata ggcccatact ccttttgttg catttgctga tcgaatagag
     1621 tttccaactt tcgcttttgc accgtgacga ttatagacac gactatagtg gttagaatga
     1681 cctgtttttc tcgtacaagt tgtatcacta taataaaccc atcttccaga gctatgatta
     1741 attgctgttc tagaaatttc ccggtctact ggtaaatttg acttaataat attaccttta
     1801 gcatcaaaat gaatctctcc tggctttaaa gatttgtaat agatttcacc gtgaactgaa
     1861 tttgcttcca cttcagatga catagcgttt cctccaacaa aacctatccc tagtgcacaa
     1921 ataataactg cttttttcat aaaacgacat cctttccttt ttgattttta aagcatgatc
     1981 ttcttgtttc ctattttggt cagatatgga tcatgcgtga ctacgataac taacgtacct
     2041 tgggaagctt gttgcgataa gatttcaact actttatcct tatttctttt atccaatgac
     2101 ccagtcggtt catcggctaa aatgattttg ctattcttca ataagactct cgcacaggcg
     2161 attctttgct tctcaccacc agataatgtg tagataggcg tacttagctt cttgtttata
     2221 tttacttttt tcaatgcctc atactttgct gttcgcttat ctgaaacatt taaattcgaa
     2281 aacttcaatc caatatctaa attaaaatcg attgtttcat attcaatcaa cccaaaatct
     2341 tgaaaaagaa aagatatggt atttcttttt atcaagcgct tctctctccc agttaattgt
     2401 ttcgtctctt gatcgaacaa atagaattgt cctgaactga actgatcatt caatcctaat
     2461 atattcaata acgtactttt acctgctcca ctttctccaa caacagtcac aagatcccca
     2521 gcatccgcag tgaaacgatc caaatgaaga atcgttttgt ttcgaatgat ttttttcgca
     2581 ccttttatca ctatcaacgg ctccaattgt taacctccat tcatcagcaa taactgactt
     2641 ttcttttctg tagacaaaat aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa
     2701 aaaatattaa aaagatattt aggctctctt tctataagaa aaaggaagat tacacttgtc
     2761 gttccccatt tgatgaggtt catcatgaag aaatctttat acgtttcaaa gagcgaataa
     2821 ccacttgaac gaaagatagc gattttccga caattgtaga caaaatatat gcataacatg
     2881 acatagctca acaagaagaa agaaaagaaa gtgagtaaaa atatctgtat caagtaatga
     2941 aatgatctgc ttaacacttt gacagctatc tcttcagtct gagtatacgg aaatgataac
     3001 tgcaagttat cacgtaactg actttcttca atcagctcaa ataacctttc tttcgacaaa
     3061 gagcctatct tgatctttaa cggtggatag atttctccgc tgagaatatt tctttccaaa
     3121 tacatactgt ttttcaatgt cagtatttca acaaccggat aatgttcgat ccaggggata
     3181 gaaggaataa atgtataaat gggttgattg ttttttataa atagaaattt cggttctttt
     3241 tcactgattt cttgataata ttctgttatt tgcgccaggt cattttttaa atctgtatgt
     3301 ctatatcgtt cagggatcaa tataacaggg tgcgtttctt tttcggttat ttggatcaac
     3361 cttccagaga catcaatcaa aggaaatttc ttaagataat tcagatttat ttttatccct
     3421 ctgccaaact gttcattttc ttcattcaag taatctgata cattgactaa tatacttccg
     3481 tttttattca aagtttcata taattcctga tctgctgctg atgcaaaacg attggaataa
     3541 ataaaatcca cgtggttttt cccgatggtt actggataaa atacatgata gtcctcacca
     3601 gcgatttctt ttggtggttc atgggaaaca gctaactgtg aagaagaaaa aacgatagtt
     3661 gctaattcga cattggcaac ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga
     3721 atgtattgga tatagttgaa cgcttctctt aacgaagcga ttttttcaac taataaaaga
     3781 gaaatcaaag agataccata tattccgctg aacaagaaaa cgtacatctg gaacaaataa
     3841 agaaattgct ccttataata cataaaaata aaaccaccaa tgattcctgc aattatgaat
     3901 gtaataatga ttttcttttt ccccaataaa cacca
//
LOCUS       DAFGFZ010000175         3875 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000175 DAFGFZ010000000
VERSION     DAFGFZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.178"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            164..1144
                     /locus_tag="LR726_002823"
     CDS             164..1144
                     /locus_tag="LR726_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL6217839.1"
                     /translation="MAKKKQDLINGTFREKKRNLKKIMAFGDGRISGKTLENMKACGS
                     FMEFIATVDKEKKKMVHGMFCKNRFCPVCAYRKARKDSYMLSIMMEAIQAEKGYEYIF
                     MTLTTPNVPADKLSEEIDRFNKALSKLFRRKRVMTSIKGYVRKLEITYNKSRNDYNPH
                     FHLIMAVNKSYFTDKRYYIAQAEWLDMWRDVTGLDGINEDGTDEICSLDVRKVNGYRQ
                     EKAVSEIAKYSAKDFEMTYSQDVFDTFYFAMKGRQLMTFNGVFKEYRKKYDAGELDHY
                     RQKDENDYFWFLTASWMQKDANYSVAVRELTDDEKKFFAERYDKEGDETG"
     gene            1116..1379
                     /locus_tag="LR726_002824"
     CDS             1116..1379
                     /locus_tag="LR726_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL6217840.1"
                     /translation="MIRRVMKLDKQRLYEAVKDNCNCGGNRQLFKAMMVTVYRRLDGE
                     NAEAAVENAYFQVFGEHHSPEWDRAILDDMAEMHEKLWRALND"
     gene            1432..1794
                     /locus_tag="LR726_002825"
     CDS             1432..1794
                     /locus_tag="LR726_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217841.1"
                     /translation="MGVTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            2016..3422
                     /locus_tag="LR726_002826"
     CDS             2016..3422
                     /locus_tag="LR726_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL6217842.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELGYISRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSENEIEW"
BASE COUNT         1267 a          584 c          945 g         1079 t
ORIGIN      
        1 aaaaaaaagt tgcatatccg tacctagtgt tgtatgcttt gagtgtctaa gtcaatagtc
       61 ataaaacaag agaggagtgc aacctttttt tcgtgcattt agaaaatgcg ttttttaaat
      121 gtttgggcgg aacgtttgtg ttctgcaagg ttcagtatag catatggcga agaaaaagca
      181 agatttgatc aatggcactt tccgagaaaa aaagagaaat ttaaagaaaa tcatggcatt
      241 tggtgatggt cgtatttctg gcaaaacctt ggaaaacatg aaagcctgtg gatcattcat
      301 ggagttcata gcaacggttg ataaggagaa gaaaaagatg gttcatggca tgttctgtaa
      361 aaaccggttt tgtccggttt gtgcataccg aaaagcccgt aaagacagct atatgttgtc
      421 tatcatgatg gaagctattc aagctgaaaa gggctatgaa tatatcttta tgacactcac
      481 cacgcctaac gtgccggcgg acaaattatc cgaagaaatt gatcgtttca ataaagcact
      541 gtcgaagcta tttcgtcgaa aaagggtgat gacttcaatc aagggctatg tccgaaaatt
      601 ggagataacg tataacaaaa gccgaaatga ttataacccg cattttcact tgattatggc
      661 tgttaataaa tcatatttta ctgataaacg ctattatatt gcccaagctg aatggctgga
      721 tatgtggcgg gatgtcaccg gtttggatgg cataaatgag gacggtaccg acgaaatttg
      781 ttcgttggat gtccgcaagg tcaatgggta ccggcaagaa aaggcggtat cggaaattgc
      841 taaatattct gccaaagatt ttgaaatgac ttattcccaa gacgtttttg acacgttcta
      901 ttttgctatg aaaggtcgtc agctgatgac tttcaatggc gtgttcaagg agtacaggaa
      961 aaaatacgat gccggtgagc tggatcatta ccgccagaaa gatgaaaacg actatttctg
     1021 gtttttgact gctagctgga tgcaaaagga tgccaattac tcggtggccg ttcgtgagtt
     1081 gacggatgat gagaagaaat tctttgcgga acgctatgat aaggagggtg atgaaactgg
     1141 ataagcaaag gctatatgaa gcggtgaaag ataattgtaa ttgtggcgga aatcgtcagc
     1201 tttttaaggc tatgatggtg actgtttatc gtcggttgga tggggaaaat gctgaggcag
     1261 ctgtggaaaa tgcttatttt caagtgttcg gtgaacacca tagccctgaa tgggatcgtg
     1321 ctattcttga tgatatggcg gaaatgcacg agaaattgtg gagggcgcta aatgactaat
     1381 caaattggtg ttatttatag gaatgaatct aagggtaagg agttgtaatt catgggagta
     1441 actttagctg atattaaagc acaagaagaa aaattgaaaa aagagcaggc aaaacttcgc
     1501 caacagaaaa agcgattgat tgagaaggaa aatgctgaaa tgggcaaggc gcttcgcaag
     1561 cattttgggt ttgaatcttt tgctgatttt gagcggtggc tatcgtcgat ttctgacgat
     1621 cagcaacacg aaaaatcagt ggataatgag tggcttgctg atgaaaaatc agcgccaagc
     1681 gaacgagatt atcccgatga atctgacggc ttattcggag tgataccgaa taagaaaagt
     1741 cagatgagtg agagtaaatt tacgtcaata tttaatgacg tgaatttacg ataagcgatt
     1801 attttgcgtg aagcaaaata aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc
     1861 cttttttcgt tgtcacttgt gacagcgaat accctcggag agcatttgga attgtgacag
     1921 ccatgtcaca attaggaaaa tgttactctt tgacaaagag taacagaaag gtttacactc
     1981 tttttattag cgacagcgcg aaggaggggg ttaacttggg gtttagtatt tcgttcaaaa
     2041 agggaacaaa gaatacaagc atttctcata ataatcgtga gttaactgat agccaaaaaa
     2101 tcaatgactg gcataagcat attgatttca caaaatcgga tgaaaatatc tatttggaac
     2161 aaataaatat tcgtgaaaaa tatgaagaat tattcggtga agccgtagaa aaatataatg
     2221 caaagcagaa aagagccgat agaaaaatcg aggactattt agcaaaggtt cgcaaggata
     2281 aaaaaatgga accacagcga gaatttatcg tgcaaattgg tactcttgat gattttcgta
     2341 ctactcgtga tgatggttct tctactggca tatcggaaca acaagcagaa caaaatcgag
     2401 tgattgccaa caaaatttta gttgagtatt tcaaagaatt taaagaacgg aatcctagtt
     2461 tatccgtcta caatgctgtt attcataatg acgaagtaag tccgcacctg catttgaata
     2521 ttgttcctgt tgctgaaggg tataaacggg gtgttcaaaa gcaaccaagt tttaacaaag
     2581 ccttgcaaca gcagggttta cgagcagata aagagaatgg tcgagtgatt tggaataatt
     2641 ttaggaatca ggaagttgag tcaattgaac gtttgatggc agattttggt tgggagcgtg
     2701 agttagtcgg tactaacaaa atcaaagatg ttcacgaata taaagaaatc atgagcgaga
     2761 tttcagaatt gcgtcaaacg gccgtaaaag agcgtgagag cgtttctgat gagttggggt
     2821 atatttctag gcaaagagaa gaaaatgagc gagaaaaggc tctggtggct tctgagagag
     2881 cgaagattga agaagagaag aaaaatatgc tgaatgtaaa aaaagagatg gcggtcattt
     2941 tccaaaaaga tattgttcct ttagaaagtt tcaagaggct tcctgatggt tcgtatcatt
     3001 tatctgggaa tgattttgtt gatttgtatc aacgagctac gagagcaaca aatgccaaga
     3061 gaaatgctga taacatgagt gagcaatatt tacagatgct tgagcaaaat acagaattaa
     3121 atgagtatgt agctgcattg gaagaagagc gtcatttgtc tggtagaaaa caattagcag
     3181 atttacaagt ggaaaataaa aagctgaaag aagaaaatcg ctctttacgc cagcgtttag
     3241 agcgattaaa aaacgcattt caaaaatcga tcactcgttt gtctatccgt tttggtcttg
     3301 cagaaaagga tatggggctt tctgaggaat tagaagtatt agagcaggag aaaaccttat
     3361 tttatcgtca aaacgtctca cagcgtaaat tagagcgttc tgagaatgag attgagtggt
     3421 agccacgatg tgcagtcaac ggtaccggag gcggtttgtg ccgaggataa ggagttgacg
     3481 gctaaaccac tagcctatta agctgacatt tgctaaaaaa tggccagtcc attttttggc
     3541 caacttagcg ttagcgaagt ttgggggcgc cctttttaaa gggtgcggtt ttatgtttgc
     3601 atgactaaga atgagtggga aaaacttaac cgcatttcta acaggcggtt aaagtatcaa
     3661 agtttgaccc ttcccttaaa aatgcagtgg tcaattgacc gaattttatg cagaagtggg
     3721 tttttgagtt ttggggtttg aaacgagaaa aaagaggtcg atttcgacct ctttttttga
     3781 tgtttcttct tatcttgata catagggtaa ctattttcgg cagaaaatcg ggtcctatgt
     3841 atgtgttttt cccttgacct ataagggttt taaac
//
LOCUS       DAFGFZ010000176         3660 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000176 DAFGFZ010000000
VERSION     DAFGFZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.179"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            197..460
                     /locus_tag="LR726_002827"
     CDS             197..460
                     /locus_tag="LR726_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL6217843.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..732
                     /locus_tag="LR726_002828"
     CDS             460..732
                     /locus_tag="LR726_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL6217844.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            809..1138
                     /locus_tag="LR726_002829"
     CDS             809..1138
                     /locus_tag="LR726_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL6217845.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1128..1415
                     /locus_tag="LR726_002830"
     CDS             1128..1415
                     /locus_tag="LR726_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL6217846.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1699..2556
                     /locus_tag="LR726_002831"
     CDS             1699..2556
                     /locus_tag="LR726_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL6217847.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            2557..3105
                     /locus_tag="LR726_002832"
     CDS             2557..3105
                     /locus_tag="LR726_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217848.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            3365..3660
                     /locus_tag="LR726_002833"
                     /pseudo
     CDS             3365..3660
                     /locus_tag="LR726_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1377 a          472 c          739 g         1072 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac
      721 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
      781 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      841 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      901 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      961 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
     1021 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
     1081 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
     1141 tcgttccttc aaaacatgtc attaagtatt taaaaaagct aaaagagaag ccattgaaag
     1201 aaaaattttt gaatttaatt tatgatgaaa ttgcgattga tccatataag ggtgaacaaa
     1261 aaactgggga tttatcaggt atttggtcta gtgggttcaa atatgcgggc acaacatata
     1321 gagtagctta cgagattata gaaaacaagg ttattccagt attgttatgt ggaacgcatg
     1381 aaaactttta tgaacagttg aaaaaattaa tctagtgatt tgttgtcgtt cttaagaagg
     1441 acggcttctt tttatcgaaa atctgattcg tctacatgac taaagtcgcg agtattctct
     1501 gcttctcgat aaattaaata acgtcattac tttgaggaaa gctataaatc aatatatttc
     1561 agcttttcct tatttatgta ctacctttta cttattaatt ataaggtagt acatatatat
     1621 ctttttttgg tagtaactaa aaataaatat gttataatag tatcaaatga atattgctaa
     1681 aagaaaagag gtaggaacgt ggtagcgaag aaatatgtaa tcggtaattt taaaggtggc
     1741 gtaggtaaaa gtacgtgcgc gcaaatgtta gggtttgaat cagcagttaa tgaaggaaaa
     1801 agaaccttga taattgactt ggatatgcaa ggaaatactt ctgatgtgat gaacttaacg
     1861 catatgaact tcactaacga agaaggaggc ggagaaggtg aaccgattgt ttacgaaaat
     1921 actgtgactg acgtgttgat aaacaatctt ccacctaaag aggctgtcta taaagtaatc
     1981 aataacctct atatacttcc agcagacatg tcctttgaaa tgtatgatga ttggatcaaa
     2041 gaccaatata atgattcatt atcccaattc aaatatatga aagaaaagct agaaccatta
     2101 tttgatgagt ttgacgtcat ctatttagat gtcccacctt ctatttctgt atatagtaaa
     2161 tccgcgatgt acattgcgga ctgggcaatc gtcgtcttac agacccaagt aaaatcgatg
     2221 agaaatgcga tgcaatatct tgagtatatg gacttttttg tgaaagaatt tgatacaaat
     2281 ttatatgttg ctggtgttat tcctttcatg ttggaaagcg gtgacgcagt agatcaggaa
     2341 atgtataaac aagcccaaga gatttatggg gaacatttga ttaaaaatgt tgtactcaaa
     2401 aatgcgcgct taaaacgata cgacggatca ggtattacga tggagaagac aaagaaaggt
     2461 ctcttaaaac aatgggatcg caaaagccac gaattattta tcagtatttt gaaagaattg
     2521 gaagaacacg agaattggta tgcggaggtg gagtaattgg ctgacatcaa aggacttgat
     2581 atttttggga acaacaaagg actcgccaaa ataaaaaaag aagcagaagc aaagaaaaag
     2641 cagcaagcaa ttacaccatc tgaaaataag caattagaag tgcaccaagt tcaagaaata
     2701 aaaacttcct ccaatgttga aagacagagt agaggaagaa caaaaaaagt tggcgcacca
     2761 atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc gactttgtta
     2821 aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag agatgaattg
     2881 cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga taagcaagat
     2941 ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc aattgttcca
     3001 gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca agataccgaa
     3061 gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc aaagaagaac
     3121 taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat gaaatagagg
     3181 ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag gaacggttat
     3241 gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta tgataatctg
     3301 ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt aggaggagat
     3361 gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg tgagtatgtt
     3421 tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag aaaggattat
     3481 gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt cagtttaaag
     3541 gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg tgtaaaagtt
     3601 atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa gcgagatcaa
//
LOCUS       DAFGFZ010000177         3617 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000177 DAFGFZ010000000
VERSION     DAFGFZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.180"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..303)
                     /locus_tag="LR726_002834"
     CDS             complement(<1..303)
                     /locus_tag="LR726_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6217849.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTI"
     gene            complement(419..985)
                     /locus_tag="LR726_002835"
     CDS             complement(419..985)
                     /locus_tag="LR726_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HBL6217850.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1121..1774)
                     /locus_tag="LR726_002836"
     CDS             complement(1121..1774)
                     /locus_tag="LR726_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217851.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1824..2252)
                     /locus_tag="LR726_002837"
     CDS             complement(1824..2252)
                     /locus_tag="LR726_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL6217852.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2257..2631)
                     /locus_tag="LR726_002838"
     CDS             complement(2257..2631)
                     /locus_tag="LR726_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217853.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2935..3120
                     /locus_tag="LR726_002839"
     CDS             2935..3120
                     /locus_tag="LR726_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217854.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3133..3381
                     /locus_tag="LR726_002840"
     CDS             3133..3381
                     /locus_tag="LR726_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217855.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3412..3582
                     /locus_tag="LR726_002841"
     CDS             3412..3582
                     /locus_tag="LR726_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217856.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT         1047 a          645 c          630 g         1295 t
ORIGIN      
        1 tatagtgttt aatatattat tcgtaggata atatgtagat tccttaactt gtcttttgta
       61 tataactaac cattcttcaa caagctcatg aaatgttaga tcaggcttat gaagtgtttt
      121 ttcgtttaat tttatatcaa tttttttatt taactctagc aatgccactt tctgagtttc
      181 tcgagattta tttttataag taatgctcac tttttttctt tttctagttt ttggatcaat
      241 atatcgctca ttatatttat atactttttt tccgttttta tcagttttag tttcgatcca
      301 cattgttttc atctcctatt tgttgctata ataggaatag ataagtaagc cgattatagc
      361 aggtttattt ttcatcacgt ccacaaactt tggcgaggga ggggcgtgtt tttgttagtt
      421 attgtggtag gttatctaaa gcgtattgtg cttgctcatt tgtaaaacct tcaaatataa
      481 gttgatcgta caattgcgaa tctgacattg atgcccaatc atcgtaactt tccgctttag
      541 ccaaagcttg ttcgttgtaa tcgacatcaa cattagccag cgcataatcg attgcatcac
      601 ttggatattc ttcaaactct agttgttcac gcaaaccttg ttcagacata ccagcccaac
      661 ctagatagct ttcagctttg ctcaatgcat ttctgtattc acgaggaaca ctttcttctt
      721 gagtaacttc ttctatagta gtttcagtag tttcagtttc ttcttcaacg actgtagatt
      781 ctgctaccga ttctgcagaa gacacttctg tcacgctttc tttagttgtt tcagcgacgg
      841 tatttttagc atcatcactg cctccttgcg aacctatagc aataaacacc acaacaaccg
      901 ctaacaacca aaaccatacc cgtttataaa acggtttctt aactttatac attttcccat
      961 cttgacccat aacttttttt gccatttaaa tatccctcgc ttcttgttat aatatatttg
     1021 tgatctcaga aatgaggtat gagtccgtgt tgcagcacgg gcttttttct ttataacttt
     1081 tttagagatt ataggcaaaa tagtagggca taaaaatgta ttattgaatt ccgtatttag
     1141 aaaatcctaa ttgaacttca ccggaagtct tttgctgtgt tgtacgcaat gcttcttctt
     1201 cagacattcc attctgtact ttccatgcaa caggcgacat cccgtatttg ttaacaaaat
     1261 cagtaagtga taaagtgtca gcgtcttgct gagcgcttgt ttgttggtct tctggattct
     1321 gttgagatgc tgcttgttgt tctttctgat cttgactgat aatattgcca gcatcatctg
     1381 tagtcaatcc attttcataa agggccacgc cgaaagcttc ccactctttg ttggaccaat
     1441 ttgcacgatc agctggagtt gactgtaaag tgcgttgttt catctgttca tatgtttctt
     1501 cttgaggtgc ggtttggatt gtactctgac tggagcttat aactgttggg ctaggttccg
     1561 ccgtagcttg gttggagctt gtaactgttg aactagaatc tgttttagat gtagatttgc
     1621 tagtagatga actggtttca gttgtttctt ttgttttact tatttttgtt tcttggttag
     1681 aagtggtatc tgttgattca gcttttttat tatttgaaca agctgaaagt agcagagcag
     1741 tacttaacaa caacataacg ctaacttttt tcattttata attcctcttt ctcgttgtaa
     1801 tatgtgtgct aacacgggct tttttatata agaaaacgat aagcgctttc tggaagcccg
     1861 taaagattct ttaattcctc gattttttta ggatattgat cattatcttc tttataaaga
     1921 gaaacaatga gattagcagc aaagcaatta gcttcgcttt cagatttgct tctagatgtt
     1981 cttgttgata cataatagct ggataagcca cgatgaaaaa tagcgtgacc taattcgtga
     2041 gcgcaaatgt agaatctttc ctcagagtct cgcagttcat catttaaaaa gattatcgca
     2101 cgatctctaa tttcttgaaa ctgccccttg ggattctcga taaaaggaac gtattgaatt
     2161 ttaatgccca tcttttcaca aatataaaaa ggattagcgg actggtattt ccgcttcaac
     2221 tcctcgacta aattaatcgt atccatctcc ataagctcac atctttttgc ctttttcttt
     2281 gtcttctttc acaatatccc agaaagtcgc tatcaggata tcttttacgc gctgtatttg
     2341 ttcgggtgtc aatgtttccc caccataaga catattaaca tttgagtcta gtagtttatc
     2401 aagttcaacc acttcctctt ttgtagccca cttgggaaca ttattatttc ccaataaata
     2461 atcagttgtg acaccaaaat aatcagcaac tttctttaag ttctcagatt ttggcgaggc
     2521 tttatcccat cttcttattt gtccattaga gatgcccacc tgtctttcta cttctgctat
     2581 agtcacatgc ttttcgtctg ctaattcttt aatcttagta actaaactca ttattatcaa
     2641 cctttcaaag ctgaaaagaa aataaatagc ttaaaagtta tatttttggt tgacaattag
     2701 cttttaagct agtatattta attcgtaagc taaattgtta gctaaataag agcaacaaaa
     2761 aactctacta atttaaaaca ttctctcggt cgccaaactt agaaatgtta ttttagaggc
     2821 tttttataag tcttatttaa ctatgtattc ataatagctt aaaagctaat aggtgtcaac
     2881 gatttagcta attttttagc ttacaaatta tttgtttaga aaggagctat ttttatgtct
     2941 gagaatttag acttaaaaat tcgagcggag atgagaaaaa gaagaatgac tttcaaagaa
     3001 ctagctgcgc ttatcggtat ttcaggagct tatttatcag atattctaaa cggcaatcgt
     3061 gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa aaatattgga catccgatag
     3121 ggggggtagg cgatgacaaa actaaaaaaa caagattttg taaaaaaata taattattct
     3181 ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata cagcaatttt ctcagcggcg
     3241 tatgaacggg tcacaggaca agaagtttgg atcaatacag aattatacga taaatttttg
     3301 tctttcaaat cctataacag gttacgcaca agaaaggtaa cacctaaaga atttatcgag
     3361 aagcatttag ttgatttata aaaaacagaa cattttgaga ggtgaaggtt aatgggtaaa
     3421 ttcaacagag cattagtatt cagcgcaccg ctaatcgtct atgctttagg gctttgggga
     3481 agtagacaag cgttgatagg aacgattgtg tacatggttt ggatttttat agggctggat
     3541 gaagctgagt acaaaacaaa aaagccagtc gggagggact gactaatgag taaggacgat
     3601 attgtatttc tgttaat
//
LOCUS       DAFGFZ010000178         3557 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000178 DAFGFZ010000000
VERSION     DAFGFZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.181"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..1119)
                     /locus_tag="LR726_002842"
     CDS             complement(<1..1119)
                     /locus_tag="LR726_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL6217857.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVA"
     gene            complement(1341..1703)
                     /locus_tag="LR726_002843"
     CDS             complement(1341..1703)
                     /locus_tag="LR726_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217858.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1707..2045)
                     /locus_tag="LR726_002844"
     CDS             complement(1707..2045)
                     /locus_tag="LR726_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217859.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(2024..2443)
                     /locus_tag="LR726_002845"
     CDS             complement(2024..2443)
                     /locus_tag="LR726_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217860.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2436..2669)
                     /locus_tag="LR726_002846"
     CDS             complement(2436..2669)
                     /locus_tag="LR726_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217861.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2672..>3557)
                     /locus_tag="LR726_002847"
     CDS             complement(2672..>3557)
                     /locus_tag="LR726_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL6217862.1"
                     /translation="QTIKLNWSIDRITIVGKLNQNIFYHTQHDVLILDFEQLMRVNEG
                     NGYLKAVGNNGWQLLDQHDENIAYIEISKWQEGKGRIDFNPNKISRFLADSMKNFIHD
                     LFLEPHFSRADVACDLIDVPDEFITQYRIIDPVSFKLIYGRSGKLETVYWGSRASERQ
                     IRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRGKATEWYAMVKESLDSFVSPHFLPT
                     DIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLLKRESQNDELTNHLRETFEESADD
                     LKKELDTWLQGLDVTEEE"
BASE COUNT         1012 a          795 c          471 g         1279 t
ORIGIN      
        1 agctacatac tcatttaatt ctgtattttg ctcaagcatc tgtaaatatt gctcactcat
       61 gttatcagca tttctcttgg catttgttgc tctcgtagct cgttgataca aatcaacaaa
      121 atcattccca gataaatgat acgaaccatc aggaagcctc ttgaaacttt ctaaaggaac
      181 aatatctttt tggaaaatga ccgccatctc tttttttaca ttcagcatat ttttcttctc
      241 ttcttcaatc ttcgctctct cagaagccat cagagcctta tcacgctcgt tttcttctct
      301 ttgcctataa atatacctca actcatcaga aacgctctca cgctctttta cagccgtttg
      361 gcgcaattca gaaatttcac tcatgatttc tttgtattcg tgaatatctt taattttgtt
      421 ggtaccgaca atatcccttt tccaaccgta atcattcatt aatcgttcta tagagtcaac
      481 ttcttgattt ctaaaattat tgaaaagcac tcgaccatta tctttatcag cgtgtatccc
      541 ttgttgaacc agtgctttat taaagctcgg ctgcttttga acaccccgtt tataaccttc
      601 agcaaccggc acaatattca agtgcaggtg cggacttatt tcgtcattat gaataacagc
      661 attataaaca gccaaattag ggtttctctc ttgaaactcc ttgtaatact gaaccaaaat
      721 tttattggca atcactcgat tttgttcagc ttgttgtttc gatatgccag tagaagaacc
      781 atctgctcga tttgtctgga aatcatctaa aatgcctatt tgcacaataa attctcgttg
      841 aggttccaac tttttatcct tgcgcacttt cgctaaatag tcctcgattt ttctatcggc
      901 tcttttttgt ttcgcattat atttttctac cacttcgcca aacaattcct catatttatc
      961 tgctagattc gtctgctcta aataaatatt ttcatccgat ttcgtgaagt caatgtgctt
     1021 atgccaatca ttggttttct ggcgatcagt taactcacga ttattatgag aaatacttgt
     1081 attcttcgtt ccttttttaa atgaaatact aaaccccaag ttaaaaccct ccttcgcgct
     1141 gtcgctaata aaaagattgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     1201 taattgtgac atggatgtca caattccaaa cgctctccga gggttttcgc tgtcacaagt
     1261 gacaacgaaa aaaggagccg aaagcgtatc acgctatcaa ctccttttat gctttatttt
     1321 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     1381 ctctcactca tctgactttt cttattcggt atcactccga ataagtcgtc agattcatcg
     1441 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgtt
     1501 ttttcgtgtt gctgatcgtc agaaattgac gataaccacc gctcaaaatc agcaaaagtt
     1561 tcaaacccaa aacgcttgcg aagagccttg cccattgcag cattttcttt ctcaatcaag
     1621 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     1681 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     1741 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     1801 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     1861 agatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     1921 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     1981 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     2041 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     2101 gaagcaaagt caatttttgt tggtcacgaa tcatttccgt taattcagaa atatgcctat
     2161 ctttttccgc taattgttca cgcaaaaatt cattttcttt ttctaacggg aaatcattgc
     2221 ctgcatcatt gaccggtaaa tcaccagtca attgaccacc ttttgaccga taaattgacc
     2281 gcacaacacc aatcaattcc ttgtctaaca tgataacccc attatcttta taccgagcaa
     2341 ggtcataacc tgcattgacc agttgattgc gcactgtatt tttagatata cccaactcat
     2401 cagcaaggtt ttttatggtc attttttctg gcaaatcagt cattatcttc tccgttactt
     2461 tcagctaccc cataaacata tttaacagca tgcttaggga taatgattgg ggaatcaatt
     2521 ttattatttt tcaaaatcaa ctcatactgc gttacatcaa gtaacttacc ggacattttt
     2581 ttgctatcca aaaaaacaac gacaacgtta tcacctgtta aattcaccaa ccaatttttc
     2641 ttaccaaaat gcttttgttt ttctgccatt actattcctc ctctgtcaca tcaagtccct
     2701 gtaaccaagt atccaattct ttttttaaat catctgctga ttcttcaaat gtttctcgta
     2761 aatgattcgt caactcatca ttttgacttt ctcgctttaa taaatttcga tatttatatt
     2821 tcgagtttct tgacaaaatc gcccaattag aatgattagc taacaatcca tcaatcataa
     2881 ctttatccgt aggttttata tcagttggta aaaaatgtgg actcacaaag ctatccaaac
     2941 tttctttcac catagcatac cattccgtag ctttaccacg tcttaactgc atttctaacc
     3001 gccaccaagt attaatttct gaaggcacca cctttctttt catttcttgc tctatttttt
     3061 tattgtacaa acgaatttgc cgctcgcttg cccgactacc ccaatacacc gtttccagtt
     3121 ttcctgagcg tccataaatt aatttaaatg aaactggatc aataattcga tattgcgtga
     3181 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     3241 ctaaaaataa atcatgaata aaatttttca tggaatccgc taaaaatcga ctgattttat
     3301 taggattaaa atcaatccgc ccttttcctt cttgccattt agaaatttca atataggcaa
     3361 tattttcgtc atgttggtct aatagctgcc aaccattatt tccaaccgct tttaaatagc
     3421 cattgccttc atttacccgc ataagctgtt caaaatctaa tattaataca tcatgttgag
     3481 tatgataaaa aatattttga ttcaactttc caacaatggt aattcggtca atcgaccaat
     3541 ttaattttat agtttgt
//
LOCUS       DAFGFZ010000179         3504 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000179 DAFGFZ010000000
VERSION     DAFGFZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.182"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(230..934)
                     /locus_tag="LR726_002848"
     CDS             complement(230..934)
                     /locus_tag="LR726_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217863.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1121..1366)
                     /locus_tag="LR726_002849"
     CDS             complement(1121..1366)
                     /locus_tag="LR726_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217864.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1383..2234)
                     /locus_tag="LR726_002850"
     CDS             complement(1383..2234)
                     /locus_tag="LR726_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL6217865.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2400..3404
                     /locus_tag="LR726_002851"
     CDS             2400..3404
                     /locus_tag="LR726_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL6217866.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1086 a          600 c          684 g         1134 t
ORIGIN      
        1 ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat atcttcaggt
       61 agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag gtcaagctga
      121 tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt attatatgac
      181 taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt tatcgatcag
      241 taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg actttgtatt
      301 tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa atagttgcta
      361 atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca ttttgttctg
      421 tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat
      481 tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc gttttttctt
      541 ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca gttggttcat
      601 cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga ttgtcacgga
      661 atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta ttgaagttgt
      721 catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat ccgatcgtta
      781 agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc
      841 tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa ggacggaatt
      901 gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc tcataccctt
      961 tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac
     1021 gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa ataaaaaatg
     1081 gaacggatta tgtatatata cataatccgt tccattcttt ctattttttc ggtatcaagg
     1141 caaaaacagg tagttcaggt gaataaatga cttcatgttc gtgccagggt ttgaccatta
     1201 gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg atcatgtgtc cagtttcttg
     1261 caggaaaatg ttggattttc tctgcatcga tcaaaaagac aaaatcggaa tgaaaagtct
     1321 gggtaacttc ttccgagaga gaattcaagc gttcgattgg atccataaaa tcacctttcg
     1381 acttaaaatt gggtagtatc tgggtctttt gctttgccga ctacaccatc taactgatcg
     1441 atttgtttca tttcttcttc tgttaattca aaatcaaata attcagtatt ttctttgatt
     1501 ctgcttggtg tcactgactt aggaagagga aggaattcgt gttgcagcga ccaacgcaaa
     1561 gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt gtttcatttc aggcacatcg
     1621 aagattttac cagtgcctaa tggactatat gcttcgagga tcatatcatg tttttttgca
     1681 tactcaacta cagttgtctg taattcgcct ggtgcaagga aaacttgatt gaccatcggc
     1741 ataatttttg ctgtttttgc tagtgtatct agatggtggg gtaagaaatt gctcacaccg
     1801 attgctttga ttttacctgc ttcatacagt tcttcaaatg ctcgccatgt atctgcattt
     1861 gcttgttccc aattatcacg aaaagcaaca gggtttggcc agtggatcaa aaacagatct
     1921 aaataatctg tttggagctt cttcaatgat tcttcaaaag aagacatcac aagttcatag
     1981 ctgtggtttg cattccataa ttttgttgtc aagaatatct cttctctagg aattccactt
     2041 tccttaatcg cttggccgac gctttcttca ttcttgtatc cttgggcagt atcgatatgg
     2101 cgataacctg cagccagcgc ttctttaaca gaggaaacag cgacatcccc atcaggtgtt
     2161 tgccatgtgc cgaatcctac taccgggatt tcgtaaccat ttgatagacg ataagtgctt
     2221 gttaatgaat tcatactttc agctcctttt ttattcgttc aactataagt gtagcgcatt
     2281 tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat ttttgtttaa aagaaaatga
     2341 ttttgattct ggtatttttg attttttttc cgtaaactaa gacagaaggg aatgagaaaa
     2401 tggatatcta tttaaaaaaa gcaattctgc atattattga cagagaaact ggttctccag
     2461 tcttttcaca aaaagagctg gatctgacaa aagaatatat tcgtgatttt cttcaaaaga
     2521 agatccaaaa aatctcttct gctcaaacaa aaactggaca actagcagaa gaaagcatga
     2581 tcgttgaatc acttaaaatc gctcaagaaa attttgtgga agcaagtgaa gcaatcgttc
     2641 agcggtggtt cgatatctac caagaaagtg aagaagcacc tagtgcagat gtatttgttg
     2701 tcttgtatga attagataca gtgatgtact tatctttatt gaaagtcaat tatcgtgagg
     2761 cttacactca ctttgttgaa gcagacgaag cagggatcga caaccgcttg attttgaatc
     2821 gagcaattct aggggcgaaa acacaaaaag cagatgaagc attcgctgtg aatttgactg
     2881 attggaccta cgaattaatt gaaaaaaaat atgagttttc tggcaaaaaa gagctttatt
     2941 tttctggaag agtgatcgaa agtgttcctg caccttcttt agaagaaaat gtaaaagtga
     3001 tccaaaaagt agccaagcat cttggaaaaa aatttgagac agaagaattt gatatcatgg
     3061 ctgatttgaa agaagctgtc tatgatacga tagaagaaaa aggtcggttg gatcatgaga
     3121 tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa aatggctttt aaagaagaag
     3181 tacaagagca aggatttgtc ccagaagcac ctcctgtaaa agaagtacaa gaaatttctg
     3241 agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg gatcgaactg atcgtaccag
     3301 tagatgtata tcgcaaccca gatttgattg aatttgtgaa taatccggat ggtacgatat
     3361 ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt ataaaaatga ctagaatatt
     3421 aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc aagagaagga tcggaaaaga
     3481 cgaatataac aaaaaaagag cgtg
//
LOCUS       DAFGFZ010000180         3467 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000180 DAFGFZ010000000
VERSION     DAFGFZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.183"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..1015)
                     /locus_tag="LR726_002852"
     CDS             complement(<1..1015)
                     /locus_tag="LR726_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HBL6217867.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYL"
     gene            complement(1299..2246)
                     /locus_tag="LR726_002853"
                     /pseudo
     CDS             complement(1299..2246)
                     /locus_tag="LR726_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            2258..2731
                     /locus_tag="LR726_002854"
     CDS             2258..2731
                     /locus_tag="LR726_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6217868.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(2904..3458)
                     /locus_tag="LR726_002855"
     CDS             complement(2904..3458)
                     /locus_tag="LR726_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6217869.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1129 a          638 c          535 g         1165 t
ORIGIN      
        1 ctaaataatc tccagtaaca acaattgcag taaattcagc taattcttga taaattcgct
       61 caaattcaga gttgttatat atccaatgcg atttaaaggt taaatgatat ttctttaatg
      121 tatcgatata agcttgatgt cgttcttgaa taacacttgg ttcacctaca atgtagtcaa
      181 aagaaagaaa cgctattttc tcatgaccat aatgaatcaa atgttccact gccaatgtac
      241 tagcttggta atcatttgta tttacaaata aacgattcat ttgttgttca agttctctta
      301 atgtctcttt tgaaagatgc gtatcaatat aaacaacagg accatcaaaa acgttattta
      361 attccaaaaa ttcttttaca cgtacaccaa caacaatgaa cccctcaaaa cgccaatttc
      421 tctgaatagt tttaacatct tctgctgaat taacattatg caccaataca aaataaccat
      481 gattctttgc ctgttcctca attccagaca aaacttctgc ggtaaatgga tcagaaaata
      541 caaaatcagc attatcaata gccgaaaaat aaagcaatcc tattaaaaat gcaatcgtct
      601 taccgattcc ctgacagaca gaacacattc cactaggatc attaaatgaa taagcatttg
      661 catatcctaa actaggtgtt gcaaaacatg aaaacaacac acgaaataac gcattgatgt
      721 tagtaatcgt ccctaacgtt gagcgcgcac tgccaccaat cttcttttga ttgaccacaa
      781 tcgccgtaga aagattatca atctcatcaa cctctggacg actgtattta ggtaaaaata
      841 aacgggtaaa actatcatag gtgtcattca gttgacgacc agcttcttgg gtaatagtat
      901 caaaaacaat cgatgatttt cctgaaccag atacgctggt aaaaatacta attttatttt
      961 tgggaatttt gagactgaca tttttaagtt gtttctcttg cgtggataat ttcaatatac
     1021 tcgttcattt ggtactcccc tttattttga aatagttgag aatgtttcaa tgagacacgg
     1081 tatttttggc agatgtttag tttacctaga aagaggtata aaaatcaaga aaaaaagaga
     1141 ggctgactca taagtagttt tattagtcga aaatgatagt ttataaaaat aggaaccgca
     1201 tcaaatcaac attctttaga atggatttga tgcgtttttc tttatttttt gacttatgag
     1261 tcaacctctt actcttcgtc atggatttga ttagctaatt attgctccac ttgttcacga
     1321 atgaaattta agtactgcgg taaaaataaa ggaatttctt caaagcgtgg actttgattt
     1381 tcatacgctt ttgcatctag gtttccttcc cctgtagcaa tttcaatcaa taattgattt
     1441 gggtcacgat aataaagtga tacaaaaaat tcacgatctt ttattcctga atttataaaa
     1501 ttgcgctctt gtaattgttt gtcgatgcgt tgtaaatcag atttattttc tacgccaaac
     1561 gcaacatgat ggacgctacc aatcccttca atggcaactg gattctcacg atcttgaata
     1621 atctgaactt cttgatagaa ctgagtgtct tgattttcta agattgtcac tcgttgatga
     1681 cccgaaaaga aactgacgta ttctttgtgc taccagccta ccgacaataa ctctcgttca
     1741 gtcgcatcgg aatattgtac gcgtagttga atggcatcaa ttcctaaaat ggcatgctct
     1801 gtagggatct ggtctgtgac atacgggaaa taatcatcaa tatcttcaaa ttcgcgtaag
     1861 ggaactaaag ctaatcgtgt tgcatctggc ccttcaaaac gtaagatagg tcgtccagaa
     1921 aatgattcga ttccataatg gcaaacgcct tgtgtttcaa aatatatttc ccaaaaatgc
     1981 aatgatgctt cggaaggaac ttttagtatc gttcgttcaa tcgtattggt tccaaaaaac
     2041 tggttgttcc cttcttgtac ttcaaaaaaa gtcagttcag ttcccacacg gtggtgatta
     2101 tctgaaaaaa acaggtgata catggtatga tcgtcttgat taattgtttt cattagtaat
     2161 tttaaaccga gtatattatg ataaaagtca aatgtcggtc gaataaagcg attcacaata
     2221 gaaatgtgat gaatacgtgc gggcataata tctaaaaatg agaatcaaac ctataaaaga
     2281 tggtgaacaa cttttgtttt catcagaaga aaataagccc ctttatttag actatgtaaa
     2341 tcacaatttg aaaatcatca ttaaagagaa taacttaaaa catatcacac ttcatggttt
     2401 tcgtcatacc cattgtagtc tgctctttga atctggtgcg tctgtcaaag aagtgtatgt
     2461 tcgacttgga catacagata ttaaaaccac tatggatgat atatacaccc atatcgcgaa
     2521 aaagcagaca gaggaaacgg caattcgttt tgcagatttc atgagttcta cagaggaaaa
     2581 gcaaggaagt ttgcagaagg tcaaaagtat ggtcaaaaac gaaaaaagca gactatccct
     2641 attagatagt ctgctaaacg ttgagttatc agtatttttc ttattccgca gcgtttgcgt
     2701 ttttgagacc gtattgattc tcttttttta atttcatcct tttccaacct aaaaatgtta
     2761 tttaattact tagtttatat aacacgaacc tataccaagt accagtaacc ccagctttta
     2821 ttccgcgaac tttatgaaat aaaatcgttc tagataaaaa tagcttttaa tctttgtctt
     2881 tactgtttat aaaaattttt aaattataca cctagtttaa atccacgaag tgatgctcct
     2941 atttcatctt cagttatacc aatgtacctt tttgtaattg cttgcgagct atgattaaaa
     3001 ataaacatta aggtagcaat gtctctagtt ttcttgtagt agtgataccc aaaagttttt
     3061 cttaatgtat gtgttcctat atcatctcgt cctaattttt ctgctatatc tgcatatatc
     3121 tgatagacac gatcccttgt tatatgtcga ccttgttgcc gactcggaaa taaccaatca
     3181 ttttcttctt taccttcagt atataataaa atttcttctc tcaaattatc tataaaaatc
     3241 ggttttcgct tacctgtttt atgttcaaca ataataggct tacttttgcc tcgtatatct
     3301 ccaactttta atcctataat atctgacata cgtaacccat tattgattcc aaacaaaaat
     3361 aaaaaactat ctctttccgg attacttgtt cttcttagcc aaaactgaaa atcttgtatc
     3421 tcttcatttg tccttaacgg ttgtacatta tagctcacta ttgtttc
//
LOCUS       DAFGFZ010000181         3449 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000181 DAFGFZ010000000
VERSION     DAFGFZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.184"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(150..1337)
                     /locus_tag="LR726_002856"
     CDS             complement(150..1337)
                     /locus_tag="LR726_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL6217870.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="LR726_002857"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="LR726_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="LR726_002858"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="LR726_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL6217871.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..>3449
                     /locus_tag="LR726_002859"
     CDS             3305..>3449
                     /locus_tag="LR726_002859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217872.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          978 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttta
//
LOCUS       DAFGFZ010000182         3438 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000182 DAFGFZ010000000
VERSION     DAFGFZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.185"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..203)
                     /locus_tag="LR726_002860"
     CDS             complement(<1..203)
                     /locus_tag="LR726_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL6217873.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(640..900)
                     /locus_tag="LR726_002861"
     CDS             complement(640..900)
                     /locus_tag="LR726_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217874.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            975..1178
                     /locus_tag="LR726_002862"
     CDS             975..1178
                     /locus_tag="LR726_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL6217875.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="LR726_002863"
     CDS             complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="LR726_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL6217876.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1807..2706)
                     /locus_tag="LR726_002864"
     CDS             complement(1807..2706)
                     /locus_tag="LR726_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL6217877.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2719..3312)
                     /locus_tag="LR726_002865"
     CDS             complement(2719..3312)
                     /locus_tag="LR726_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL6217878.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
BASE COUNT          936 a          669 c          586 g         1247 t
ORIGIN      
        1 cgtttcttta ctttctcatc aatcaaaaca taatcgccat aagacaaggt atattttttc
       61 ccgcatgttg gacattgata caaacgatag cttttgatac atttcgctct ttctccacaa
      121 tcgcattcga cagtatgtgg tgcatgatag tttgggaata attcgctgaa gggaacaccc
      181 caataatcaa atttctttcg catgattgat tctgcctcct cttaggaaag gttttatttc
      241 ctttcgttag ttctttcttt attgatttgt tggccagaat gaatcaacga aaagcgacaa
      301 ggacaagggc gcgcagcgtg ccatttaccc ttgtccttgt cgctttttgg tgataaaagg
      361 caacttgttg ccggtctggc aaatcaataa agagatagcg aacaggaacg aaccttttgg
      421 tgagaggaag ttttttctat tttgtcttgc tttgcaagac accatatctt agtgaacgtg
      481 caacgttcac ttcctttctt ctcgaacggc ggtggcggtt ggagtgagag aaaggttttt
      541 caattgagaa ggcacttgtg tcttcctaaa aaggtctttt cctgtttcta tgtaaaacaa
      601 aaagagctgc aatcctcatt taggactgca gctcttcttt tatatgccca tcgatcgcgt
      661 aaaatgatcg gggtttctaa tttgcatcga ttcaatgact aattgattcc gtttattgaa
      721 atgacctgtg acggcaatat tgtattttcc atcatccata attaacaatt tatcgataat
      781 ttcaatatct gcaactacac aattaatcga ttcatgaatt gtttgtaaag taaagcgaac
      841 caataatggt tgtgttttta ttactcggat tttatcaagc aaaccaacat atttgttcat
      901 cacgatcacc tcgctaaaat tatagcaaaa acagctaaaa ttgagaattt ttattcgatt
      961 ttagatatgc tataatgatc aagacagtaa agtttgtgac ggtggctatc cgattgaaag
     1021 tgaggtggtg cgtatgtgcg tacttccaaa atcttacaga aaggagaagc cttttgcctg
     1081 ttgaaacggc gctaggatta atgattagtt tcggttcttt gatcgcaaca atcatttttg
     1141 gaatcttagc agttgtaaac aacaatcaaa aaaaataacc gtcagcttta gccggtcgac
     1201 gattattttt caaaaataat attaagctgg tcaccgtctt aaacggtgct gttaggggag
     1261 ttgttagcgc aactcctctt ttcgtatcca ttataacaac tttattgatc ttttacaatc
     1321 agaatggtaa atcatcatca ctaatatcga ttgattgtcc gccaaatgga ttttgtgaag
     1381 cgttcaatcc attgttgtta ggcgtagcta catcccgttg ttcaaaggtg gtagggcttg
     1441 gactgctgga cgattgatca gcgtgcgcac ggctttctgt cgctgcttta ctttctaata
     1501 gttggaaatt atcggccaca acttctgtca catacacacg ttgtccttgt tggttatcgt
     1561 aggttcgggt ttgaatccgt ccaacaacgc cgatcaaact gcccttttta gctaatgtcg
     1621 ccatcgtttc tgctggtttg cgccaaatca cacagttaat aaaatcagct tctcgattgc
     1681 cgttttgatt cgtgaaattt cggttgacag ctagggtaaa agtcgctact gcagtaccac
     1741 tcgcggtata acgtaaatct ggatctttgg ttaagcgtcc tactaaagtt acattattga
     1801 tcatctttat ctctccattt cttgattcat ttgttgtgat cttttaaact gccgttttct
     1861 ttcttgattt tcaattttga tcacactttt cattaggtga tcaatttcgt tgactgtatc
     1921 tgtaaatgaa atggaattcg caaacgtgtt aactgagcga tatttttccc actgcttttc
     1981 caaatagtca tttcctctga tcttatccat ttctctttca taataaaagg acaacggttt
     2041 tgtatcccca cgttttgtaa atgtatttcg aacacttttg tataactctt tcgtatcgat
     2101 attttctagt ttcggaatgg taaataagtc gtagtaatct tttgctctcg aattatctgt
     2161 gccaaagctg aacgtagcac ttagcttttc tgcaataatt tgttctgttg gataagctgg
     2221 aatctccact tttttatctt caaacattaa ttggtgatag tgtgtatcta cttcaggata
     2281 aatagaatct cctgttgtca cgtccatttt aaacggtaca cgtactttct ctaaattagc
     2341 caccaattta atttgaaatc caggataaaa atcggcttct cttgtttcct tcattccttg
     2401 aatagagaaa acaatgcctt cttttgtggg ttttctagtt aaatcattaa aaatgctctt
     2461 caatttttct tcagttaatt tgctatttct aaatgtcgta tcgatatcaa ttgttgtcct
     2521 tctatcgatt ccatattttg atcctaataa aaagccgcct ttcaaaatga agtcattttt
     2581 gtactttgat tcagaaattt gttcaattag acgatccatc ataaaatgcc gttgcactaa
     2641 ctgtgcattt aatcctgtac tctttgctaa atttcttgtt tttgctgcaa attgtgtcgg
     2701 tgtcatcttt ttcctccatc aatataagct ttgcatgtgg tatttcattt ctttttctac
     2761 atgtagtttt gtcatgtaat ctcttaattt acttggatct ctcaatgggt cttccatgta
     2821 ctcttttaat gcttctaatt tcatttcata ttcaaattca tatctcggat tccaaatatc
     2881 acaaagtgta cgctctttat catacatagt aataggattt ccttgaaagg aagttccctc
     2941 tacttttcct agttcataaa cttctttctt cacgaaatga aattgtaact gctgctcttt
     3001 tgtaattgaa cgatcaacgt tatagccatg gggaaaagtt aagtgaatga atttcggcat
     3061 attcgtagaa agctggtaaa tatctaaagt cgtttcatga gagaagattc ccctggaaaa
     3121 tcgttgctga agccaaaata aatcatctat tccatgacca tcctctacat aataaccacg
     3181 atcaactttg atcaattttc cttgattcac caatctttgt acagtggttg ctgcgattcc
     3241 aaatttcctg gcttccttca tagataaatt tccatcgcga atagccaaca attctttcag
     3301 tttatcttgc atttacttcc cctcccttcg ttacaaatac actacatcaa atatatcata
     3361 gtagtttatt tgtaacaaga ttaacgtcta tcgatctaat tcgatttctt tttgaatgcg
     3421 ctgctggttc tttaattg
//
LOCUS       DAFGFZ010000183         3398 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000183 DAFGFZ010000000
VERSION     DAFGFZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.186"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..276
                     /locus_tag="LR726_002866"
     CDS             <1..276
                     /locus_tag="LR726_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBL6217879.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="LR726_002867"
     CDS             419..640
                     /locus_tag="LR726_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217880.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="LR726_002868"
     CDS             656..1498
                     /locus_tag="LR726_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217881.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="LR726_002869"
     CDS             1561..3363
                     /locus_tag="LR726_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBL6217882.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          469 c          702 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DAFGFZ010000184         3336 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000184 DAFGFZ010000000
VERSION     DAFGFZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.187"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(451..1761)
                     /locus_tag="LR726_002870"
     CDS             complement(451..1761)
                     /locus_tag="LR726_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL6217883.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="LR726_002871"
     CDS             complement(1773..3191)
                     /locus_tag="LR726_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217884.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1033 a          676 c          515 g         1112 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataaga
//
LOCUS       DAFGFZ010000185         3319 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000185 DAFGFZ010000000
VERSION     DAFGFZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.188"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..1157)
                     /locus_tag="LR726_002872"
     CDS             complement(<1..1157)
                     /locus_tag="LR726_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6217885.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..>3319)
                     /locus_tag="LR726_002873"
     CDS             complement(1160..>3319)
                     /locus_tag="LR726_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL6217886.1"
                     /translation="ISEDGKLLHLYYGENLPDNDYSHLIEMHHRPMTTYRNENDLLYS
                     LEHLKQEFPEYGTTDFRHPAISLLQKNGSRLSDFVYQGYEVEEGKPKLEGLPATYVDN
                     NDESKTLKVYILDSVTNVQVELLYTIYRDYPVITRSARVMNKGKDIQIIENISSLSLD
                     LPDANYEWQQLSGAWGRERTIKSRTLQQGIQSIESTRGISSHQHNPFVTLKRKNTDEN
                     QGEAIGAALVYSGNFLIQAEVDTWDVTRLQVGINPFGFNWKLNPGEIFTAPEAILVYS
                     SKGLNAMSQTFHQLFRKRLARGKWREKDRPVLINNWEATYFDFDEEKLVNIAKKAFDV
                     GIELFVLDDGWFGERENDCAGLGDWHVNPKRLPRGIGSLSEKIRSFGMKFGLWFEPEM
                     VNKDSELYRTHPNWIISTPNRKPNHGRNQYVLNFGLDEVVENIFDQMCKIIDESNLDY
                     IKWDMNRPLTDVFDSHLSADQQGEVFHRYVLGVYRLYEKLITKYPNILFESCSSGGGR
                     FDPGMLHYAPQAWASDDSDAIERLKIQYGTSMLYPLSSIGAHVSIVPNHQTNRITPIK
                     TRGNVAFFGAFGYELDLNELSEGDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDN
                     QTAWMVVSHDKKTAIVAHYKTLNEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMR
                     AGLVCSDASSGQINDQNRRPETFDFDSKIWVITQEEK"
BASE COUNT         1076 a          625 c          482 g         1136 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatat
//
LOCUS       DAFGFZ010000186         3313 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000186 DAFGFZ010000000
VERSION     DAFGFZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.189"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            94..678
                     /locus_tag="LR726_002874"
     CDS             94..678
                     /locus_tag="LR726_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217887.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSIT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            689..934
                     /locus_tag="LR726_002875"
     CDS             689..934
                     /locus_tag="LR726_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217888.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            940..1662
                     /locus_tag="LR726_002876"
     CDS             940..1662
                     /locus_tag="LR726_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217889.1"
                     /translation="MKSTKWKIVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1793..2536
                     /locus_tag="LR726_002877"
     CDS             1793..2536
                     /locus_tag="LR726_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217890.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            2542..2907
                     /locus_tag="LR726_002878"
     CDS             2542..2907
                     /locus_tag="LR726_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217891.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            2923..>3313
                     /locus_tag="LR726_002879"
     CDS             2923..>3313
                     /locus_tag="LR726_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217892.1"
                     /translation="MSQNIGAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKI"
BASE COUNT         1308 a          435 c          609 g          961 t
ORIGIN      
        1 aagtttacca taaaaaaata ttaaaaagcg agacaaattt atgttattat gttatcatga
       61 tagcataaat atatggagcg agatgatgga aaaatggatt tgaaacaatc gttttacatc
      121 tggttatatc ctgataatcc tatgtcaata atggctgata tttttgtagt tagcatctta
      181 ttgtatttat tttatttaat tttctttcac aatttaatcg ggtacatgat tcttgattgg
      241 aaaggaatgt tcgatataga gagaaaacca tttgctaaca atcaaaccta taccgtcttc
      301 gaaagtggag attgggaaga aaatgaagtt gatgtgatcc atgaaattgg attagcttta
      361 tcaggaatac aagatgctga tgttttggta tcaataacaa cggatactgg atacaacgct
      421 tatgaggtat tttatcttat gtattttata tggcgtgatg gtcaagaaat tatcaatggt
      481 cgtgctgaaa aaacaatcat ttctaatact gaaatttctt tctgtatggg tgaaggggaa
      541 atgattgtat ataaagatgg gtcagcaaac attaaaacag cctttggtga tattgataca
      601 agtattaagg aacttaggga atatgtgcag aacccatata attttcaata tttagcagga
      661 agaataaatt tagtttagga gtaaaaatat gaaaaaaatt attaatcaac caacaaatca
      721 cttgataatt gaaggtgtta ttttcgtaac aatgtatttt actgtaggaa atggcatctc
      781 aaatacaatc attcaaaaaa atccacaata cctattattt agtctttatg cttggattgt
      841 gcaaatggtt tgggtttcca tattgatgct gttgttttat tggatagtac gggttattct
      901 tttagtgtta aacaaacgaa aaaaggatag gtgaataaaa tgaaatcaac taaatggaag
      961 attgtattgt tatcaacagc agcattaata actcttacag catgtggtac aactaatagt
     1021 gctgataata acaaagacac aaagtctagt agtacggtgg ttactaaaaa cagctctaca
     1081 tcggcatcaa caacaatttc ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac
     1141 gaagacatta ataaaggtca aaagccggaa gaagtttata ccgactttat taatacagta
     1201 aaaaatgatg aagattatgg gaatgatcca ttgcaaaata aaaatctgac tatcgttctt
     1261 tacaaacctg attgtccaga ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa
     1321 gaaggattta gttatgctcc aacattaatg catgatatta ccataaataa accagcttac
     1381 gagtatcacg cagttgctgt agatattact gatggtgtac ctgagtggat acaaaacatt
     1441 cctggtctgg ctgatgagaa caatcaatat catacgccaa cagttgcagt agtaacacca
     1501 aagaaagtta atggcacact atactgggat gtaatgggaa tgacaacaaa tacaactgat
     1561 gaaatgattg aaagtacaac taaatattat atggatttgg gagcatataa attatatcaa
     1621 cagtcagaac ctgttatgga cgatcaatcc aaagttaaat agttctttag ggcgtaagcc
     1681 ctattttttt aaaattaagt gataacaaat aaatgatttc tgaaacaaac gttcttactc
     1741 aactactcga aacacacaag ctaattaact agattataaa ggagaatgcg taatgaaaga
     1801 aatcagtgca aatgaagctc atattccgca aaagaatcag gttggtatat ataaggtgct
     1861 tcatggtgaa ggaatttata aaatacagtt acttaccggt tacaataacg gtgcaaatgg
     1921 gaagtggtta aaggcaaaaa gtattcgtga acttaaaact gaagtagaaa aaataagact
     1981 tgggttaaca agggaagaaa taagtagact gagtgaaaat tcttcttttg taatccaaaa
     2041 agaaaaggag aaacatctac ttcatcggga actaataatt gaaaaatact acgatgttga
     2101 tttacctgat cagttaagaa ttattgacga attttcagag aaaaagtgga aatctgaaat
     2161 atacccaagc ttaattcaat acaaggcatc aggaaagccc atgtataact acacaggctt
     2221 tttaaactac acacaatata aatatgcctt tcgcaaatta aaaggcatta aacaagctga
     2281 cattgccctg tatgagatga aaaaaatgga tagattgaaa ttgattcaag agttagtgca
     2341 aaatacatgg aatcaagcga ttgtcgaact agaaaaagaa aatgggaaaa ttttaactaa
     2401 tgaatatctg atcgtaaatg aaattgaaga attaattggt ttattttcat gtgaaaatgg
     2461 tgaagtcttc ttgaagtcat caccaatcta ttcaattttg gacagtccgt atcagtttat
     2521 acaggtaagg agatgagtta catgtctagc ttgaaagatg actatatcca agcaaaacaa
     2581 gagtatgaaa aaatacagat gttaaaatct gagaaagtcg aattattttt aacacaaaaa
     2641 ttagcagagg atcatattgc taacttagat gctaaaactt tagaggaata tgatcagcag
     2701 tttaatcaga cacatcacaa tatcattttg cttgttgatc aggcaaagag aagacttgaa
     2761 aaagcggaat atgaactatt agatcaagga attatatgct tggataaagg gattcgaaga
     2821 tattatagtg cagagttaaa aaagcaaaat gtagacagac atgcgttaaa acatgaaatc
     2881 ttagaattga taatgcaacg aatttgaagg gatagaaagt aaatgtcaca aaacattggt
     2941 gctgtaaaac aattaataga aaaagattta gagacgacag ttcagaggtt agccgaacga
     3001 atccattttg caaattatca aaccatctct aattggtcaa gcaggaacag aaatccggaa
     3061 gaaattccaa tgaaattttt ttatcgaatc ggaaaagaat atggtgttga tccgataaaa
     3121 attttcgaaa agaccatgat ttattactat catggaacac ctaacgaagt gttggaacat
     3181 cttttggaag aagcagaaga aaaatcatca atattagaga tatcacaaaa tgacaaagct
     3241 attcaagtga aatgtgttga tccaacagga aagattattg ttggatttga tattgaaaga
     3301 aaatctaaaa tag
//
LOCUS       DAFGFZ010000187         3302 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000187 DAFGFZ010000000
VERSION     DAFGFZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.190"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            125..337
                     /locus_tag="LR726_002880"
     CDS             125..337
                     /locus_tag="LR726_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217893.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(615..2414)
                     /locus_tag="LR726_002881"
     CDS             complement(615..2414)
                     /locus_tag="LR726_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL6217894.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2538..3134)
                     /locus_tag="LR726_002882"
     CDS             complement(2538..3134)
                     /locus_tag="LR726_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL6217895.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1202 a          597 c          583 g          920 t
ORIGIN      
        1 aaaagcagaa agatactcaa aataaaaaat aaaaaaatgg ataaaaacgt tttcaaatac
       61 aaggtaattt gttattataa aataaagaaa aaacaaaata aaaataaaga aaagagggaa
      121 tgaaatggta aaagcagaag taggaagatc ttatatatgc aaaccgatcg gagtatctca
      181 tgaactaatc ggctgtattg aacgtacata tatcaacacg gctgtcgtgt atgttgaaag
      241 ttatgaccaa cgagactatc aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt
      301 ctgtgatatt agcgcacctg ctgaattggt ttcttaaaca gtatttttga gaattcgcaa
      361 gaactccctt ataatataaa taaagacaga agggagcgag aagatatgcc aaaaagaaaa
      421 agttcaggga aaatgggaat tattcttgca gtgatctctg caattcctgc tattttagct
      481 attgttcgtg cgatgaagga atcaagagaa agttaagcac acattatgag cgtcaaacaa
      541 aaaacgcata tacctgcggt ggcaggttat gcgttttttg ttgtataaag acttttttca
      601 ttaacgattt ttgattagcc gactcccaaa acaaaattta gcagtttatc tgaataagtg
      661 atttcttcat ctgtttgttt cacactttca tttagtagtc ccatctcatc ggttatgata
      721 ccatccacac cataaaacat catgcgactc atagcatctg aatcgttgat tgtccataca
      781 tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat cgtactatat
      841 tccattgtca gaaaatcagc aggcgtcaca gggggcccaa caatattgaa aggtaagatg
      901 tagcccacat aaaagtttgg ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa
      961 ctttgcaaaa tatgatggtt ctctaagata actttgcggt atttatggac aaatcgttcg
     1021 acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa taagtaattt ttggtttagc
     1081 ttatgtgctt tttccaaata agaatcaaaa gaacaaacag gggcttgcac tccgttttca
     1141 gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc atttacccct
     1201 gttaagtgac gaagatttgc atcatgcatc actacgaaat gaccatcttt tgtttcctga
     1261 acatccatct ctacataatc aggatgatag tctctgcttg ttttttcaag tgcttctaaa
     1321 gtattttgcg ccccattttg ggtactcaca cctctgtgag agacagtcac tggttttgtc
     1381 tgaacagctg aagtcagata atccatattg taaagacaga cgccaatacc gaaaaaaact
     1441 gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac gcaagttggg ggcttgtggg
     1501 ataaaccact tagggatctc aggcaagaag ccttcgtcat ccatgaaatc aacaatgata
     1561 taaaaaatcc ctacggtaga catcacgata ttgaataata aaatcccttg taaaagcgtc
     1621 atagcaaaca cagcgctgat caatgcatag tctgggaaaa attgttcaac catactttgt
     1681 gccaaaataa caacgatata tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca
     1741 ataaattggc ccgtgatggc caaaagacgc gatttggtca atgaccagct ttcccgaatg
     1801 gctgctcgaa aaggacgatc ccgtagaatc atttccggta aagcaaaaat cagtcgaatc
     1861 ccaatatagc caaggaaaat ataaacgagt aaaaaggaac tgacgatgat ccaacgatta
     1921 gcaaaaataa aatccataat gaaagcgggg atttttattt ttgacagtag atcagaatta
     1981 aaacttaatc cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa caaaaaagta
     2041 atgggtctca ctttcttcag ctgaagtatc gtcaagtgta agagttgctt caaagagatt
     2101 ggttctttct ttttaataaa aaaaacactc atcaaaagaa aagtaaattc gaaataaact
     2161 aacagtaaaa tcgtcatcaa aactacgatt aacgagagta gaaccccagg atgttgagaa
     2221 aaaattgaag gaatattgtc ataagacaaa tagtcaattg cccctcggtg taaaataaaa
     2281 cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa taaagccatg catcagcaag
     2341 acatcacgga aataagcttg tgttcctttt aaaaagtccc aagcattacc taagctacgt
     2401 ttcaaataat tcatcctatc acctgctctt cacattgtag cgcaaatcta atgaaaaagg
     2461 aaagggtgca gtgtgttttg cagacccttt cccagattta acattgtcaa tcaatgacta
     2521 attttacaaa ataaatatta tccaagaaga ccacttagat attgacctcc ataaatcaag
     2581 gccatactat ataagaaaat cgacagtggt ttagcaatca aaatgattag tgtatatcgt
     2641 ttcactgaca tgtttgtcaa tccagccatc aaacataacg catcgtcagg agcaactgga
     2701 agaaaaatag ctagagcaaa cagcttctca aaacggtttt gattatccag ccagccaata
     2761 tatttgttat atgtcttatc gctaatcatg gaaagtatga atggctttcc ataacgacgc
     2821 cctagcagaa aaatgataat cgaaccaatg cagattccaa tatagttata gataaatcca
     2881 gcataagggc caaatatcag gacaccagct gcagtactga tccctccagg aatgattgga
     2941 ataactactt gcaggatttg gatgaagaca aaaataatcg gtcctaaaat gatggaatca
     3001 cctactaatc ctcttaacgc attcaaatct ttgaaaacac ctaaattaat aaaataaatc
     3061 gtcagcgcga ttgataggcc taatccgata atggatatga aatttatcag ttttcgagag
     3121 gcagcaatat tcattattca tcaactcctt agaggttctt tgttaactat actttactag
     3181 aaaagcaact aactgcgcca ttttttgata tgacagaaaa caaacaattt tgtaagttca
     3241 tcataagtat aaaagattga tctcaaaaaa acatccaact aaagacttat ttaataactt
     3301 gt
//
LOCUS       DAFGFZ010000188         3139 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000188 DAFGFZ010000000
VERSION     DAFGFZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.191"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            90..467
                     /locus_tag="LR726_002883"
     CDS             90..467
                     /locus_tag="LR726_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL6217896.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            455..793
                     /locus_tag="LR726_002884"
     CDS             455..793
                     /locus_tag="LR726_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL6217897.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            927..1370
                     /locus_tag="LR726_002885"
     CDS             927..1370
                     /locus_tag="LR726_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL6217898.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1632..2447
                     /locus_tag="LR726_002886"
     CDS             1632..2447
                     /locus_tag="LR726_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL6217899.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2457..3119
                     /locus_tag="LR726_002887"
     CDS             2457..3119
                     /locus_tag="LR726_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL6217900.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
BASE COUNT          993 a          473 c          646 g         1027 t
ORIGIN      
        1 atgagtattg acttggagta cacttaaagt tgtagaataa ataaaataca taagtaaagt
       61 ggcaagaaat taagagaaag agtgatcaga tgaatatcaa acaagtcagt gaagaaaagg
      121 gcatttcggc cgatacattg cgctattatg aacgaatcgg gttgattcca ccagttaatc
      181 ggacaaaagg cggaatccgt gattatacag aagaagacct aaaatgggtc gactttacga
      241 tctgtatgcg aaatgccgga ttatcgatcg agtctttgat tgaatatatt cgcctatcta
      301 gcgaaggaga agcaactgta tttgagcggc gtaagctttt gattgaagaa agtgaacttt
      361 tgacgaaaga aattgctgaa atgcaagagt gtttggaacg tttacaagga aaaatcaaga
      421 aatatgatcg tgtgttatct agaaataaaa tggaatgtat caagtaaaaa aatcaaggag
      481 catcgctatg gagttacgct tactaaaata tttttggaca attgcagaag aaggaacaat
      541 ctctcaagcc gcagaagttc tacatcttac acaaccaacc ttaagccgac aactgaagga
      601 gttagaagag gaactaggaa cagaactgtt tgtacgcgaa aaccgcaaaa tgatcttgac
      661 agaagcaggt tattttctaa aaaagccgag cagaagaaat tttagattta acgaatcaaa
      721 ccactcaaga attcgagaac agaaaaaaac aattattcag cggccgattt acgatcggct
      781 gtgtggaagc tgataattcg aatacattgg cactattact agaaaagttg atccgtgatt
      841 atcccagcgt tcaatttcat atccacagcg gaacaagtga tgatatcacg gagtgactag
      901 aaaaaagcct gttagattta gcgattttgc tagatccagt agatacagag aaatacgaaa
      961 cgattttgtt accaagaaag gaaaaatggg gattgctcat tttagaaaat tctgttttag
     1021 caaaaaaaga cgtgataaca aaagaagact ttgcgaatgt acctcttttg atctcaaagc
     1081 gtccagaaat acagcgaatg tttgctgact ggttaggcat aaaaaaggag cagttgctga
     1141 tcacaggaac gtacaatttg attttcaatg tatttgcgct aattgagaat caggtaggtg
     1201 ctgctttagt catagaaggt gctgtaagtc atctttcaaa gcgagtgaca ttcgttccgc
     1261 ttgacccaat gattgaaacg aactgcatgc tagtctggaa aaagaatcgg attttatcac
     1321 ctgtagtcaa tgaattgatt caccgtttca aagaagcttt tgaacagtaa taagtatctc
     1381 taatcgaaga aatttcaata caaaagcgag acgtatgaca gtcttgcttt tgtatttttt
     1441 catttttaaa tcaaaaaaaa taaagagatt tacataactt gttaaataag cgcttacaaa
     1501 cataagaaga acggaaatga atcagctata ataaccgttg ttctggattg taatcattac
     1561 aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa gcaaaaattc
     1621 gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt gtattagctt
     1681 tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca aataatgaag
     1741 tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat tatctggtta
     1801 tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta agcgttgtga
     1861 tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct aaactaaatt
     1921 ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc ccttggcagt
     1981 cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacatat acgttgtggt
     2041 cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag caatactaca
     2101 gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca gagtggggaa
     2161 tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg acgatcatca
     2221 cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct tctacggaaa
     2281 ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca gagtacgcat
     2341 taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt ttagcgatgc
     2401 aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag gtgattatga
     2461 aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg tatcaatcat
     2521 tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct gcgttccttt
     2581 tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt attggtccag
     2641 gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaaaagt ttttcaccag
     2701 tacgtttgtt cataaagacg tttcgccaat tttggctaaa gggatttttt tattggctat
     2761 ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata cgtttttctt
     2821 atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt tcctttagca
     2881 tcaactactg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa gttctacgga
     2941 tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt ttactattga
     3001 caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta ggtccttcta
     3061 tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg gatctgtaga
     3121 ctttataaat tgaaacttt
//
LOCUS       DAFGFZ010000189         3073 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000189 DAFGFZ010000000
VERSION     DAFGFZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.192"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(245..607)
                     /locus_tag="LR726_002888"
     CDS             complement(245..607)
                     /locus_tag="LR726_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL6217901.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            830..1582
                     /locus_tag="LR726_002889"
     CDS             830..1582
                     /locus_tag="LR726_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL6217902.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1722..2645)
                     /locus_tag="LR726_002890"
     CDS             complement(1722..2645)
                     /locus_tag="LR726_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL6217903.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2660..2932)
                     /locus_tag="LR726_002891"
     CDS             complement(2660..2932)
                     /locus_tag="LR726_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217904.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1006 a          581 c          507 g          979 t
ORIGIN      
        1 aagaaacgga tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga
       61 tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa
      121 gtaagaaagc agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc
      181 cttgtttttt attgtataca ttagatatgg agcaggttac cataaagatg attattagtc
      241 gttattaaat agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac
      301 gtttgaattg tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat
      361 cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt
      421 ttttcttcgt attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt
      481 gtgttcgatc taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt
      541 ccactacttt aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta
      601 gcttcatctc attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac
      661 catctttcct agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac
      721 cttttatcac ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac
      781 catctcgtta cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac
      841 aataaagaaa atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga
      901 gattggactg ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa
      961 agaagtcgat cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga
     1021 tatccaagag gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca
     1081 gcaaatgttg ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac
     1141 acttgatatg tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa
     1201 acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg
     1261 aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga
     1321 agaaatgcaa actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc
     1381 agaagatgaa agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag
     1441 ctggcctgaa tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga
     1501 acggttcact gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga
     1561 aattatccaa aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg
     1621 acctaataac aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc
     1681 tccattcttt cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta
     1741 taaggtattt tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg
     1801 caagcttcca gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta
     1861 tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca
     1921 accatatccg aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct
     1981 tgttttcctt tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga
     2041 aaaagttgat gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc
     2101 cccaacgtga tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta
     2161 attgattgtt ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa
     2221 aaaactagag gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac
     2281 gtaatgatca atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt
     2341 tctttctgat agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca
     2401 atgatcccac tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca
     2461 atgcttttcg tttttccata acttaactgt ctggcatttg cattagggat ataatcgact
     2521 tctttcatga ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg
     2581 attacacgtg aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt
     2641 gccactgctg cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg
     2701 gaccgcattc aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc
     2761 cccaatatat atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat
     2821 tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg
     2881 cataaaaata attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc
     2941 ctccttttga tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt
     3001 gccaaagaaa agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat
     3061 acattataat gat
//
LOCUS       DAFGFZ010000190         3052 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000190 DAFGFZ010000000
VERSION     DAFGFZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.193"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            14..1141
                     /locus_tag="LR726_002892"
     CDS             14..1141
                     /locus_tag="LR726_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6217905.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="LR726_002893"
     CDS             complement(1359..2501)
                     /locus_tag="LR726_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6217906.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="LR726_002894"
     CDS             complement(2506..2691)
                     /locus_tag="LR726_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL6217907.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT         1017 a          549 c          477 g         1009 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aa
//
LOCUS       DAFGFZ010000191         3007 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000191 DAFGFZ010000000
VERSION     DAFGFZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.194"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(276..692)
                     /locus_tag="LR726_002895"
     CDS             complement(276..692)
                     /locus_tag="LR726_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217908.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="LR726_002896"
     CDS             complement(695..979)
                     /locus_tag="LR726_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217909.1"
                     /translation="MDILVRNIDPKFVATIDKLCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="LR726_002897"
     CDS             complement(1679..1774)
                     /locus_tag="LR726_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL6217910.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="LR726_002898"
     CDS             2427..>3007
                     /locus_tag="LR726_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL6217911.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNSALREL"
BASE COUNT          958 a          476 c          565 g         1008 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caaagtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat tctgcattac
     3001 gagaact
//
LOCUS       DAFGFZ010000192         2947 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000192 DAFGFZ010000000
VERSION     DAFGFZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.196"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(449..865)
                     /locus_tag="LR726_002899"
     CDS             complement(449..865)
                     /locus_tag="LR726_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217912.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="LR726_002900"
     CDS             complement(867..1148)
                     /locus_tag="LR726_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217913.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="LR726_002901"
     CDS             1948..2922
                     /locus_tag="LR726_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL6217914.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAFGFZ010000193         2857 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000193 DAFGFZ010000000
VERSION     DAFGFZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.197"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            562..843
                     /locus_tag="LR726_002902"
     CDS             562..843
                     /locus_tag="LR726_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217915.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="LR726_002903"
     CDS             845..1261
                     /locus_tag="LR726_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217916.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="LR726_002904"
     CDS             complement(1422..1688)
                     /locus_tag="LR726_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217917.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="LR726_002905"
     CDS             complement(1646..2620)
                     /locus_tag="LR726_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL6217918.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DAFGFZ010000194         2667 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000194 DAFGFZ010000000
VERSION     DAFGFZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.198"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            751..2577
                     /gene="ltrA"
                     /locus_tag="LR726_002906"
     CDS             751..2577
                     /gene="ltrA"
                     /locus_tag="LR726_002906"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL6217919.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          955 a          466 c          593 g          653 t
ORIGIN      
        1 aaagggggtg cgaaacgttc ctacttgaaa agagtaggca cagcacttca ttaaatcaat
       61 atttaatgtg tgttggagaa ctaaacacac gaatagtcga atgacggggt aacgccctga
      121 aaggctactt taatactctg actggcagaa acttgagtaa acaaggctct gtcagaagct
      181 cggtgaagtc ggcagacaga caccgtaaca gataatgctg acgaaagatt cccagaggtg
      241 ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta tggttagaat gtagatgaga
      301 ggtctactga cgaatccttg aatgtacagg tctatatcgg ggctggatag aaatgtccag
      361 tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc gcatttatga aaatccatat
      421 aacgttaaag gcgttatcaa ggtagcaggc ttatagagga aacctaagga tatatgtaca
      481 gatagtgtgt ttggaacgtt gaaagctgac aacatggaga ttttactgtc tgtgaagtgt
      541 tcacatcaga aagttattgt gagattagca atgatataag gtgtgttaat gtcagtgaga
      601 gtggtggcac agtacctatg aaaccgtgat aacaagcggt ggagggatag ccactagtca
      661 tatgaagaat agaattacta agaaaaatgg tatgggttcg agtaagacta agagatgtac
      721 ttgctcctgc aaacaaggag ggtacagacc atgctaaaga aaaacaagct ccgctataac
      781 gagtattatg atatgcaaca tatttatgac gagttatacg cacagagcaa gaacggtaat
      841 aacttttata agttacttga aattattggt tcggaacaaa atatttgttt agcttaccgt
      901 aatctgaaat caaacagtgg cagtaaaacg gctggaacgg atggaatgac gatagacgat
      961 ataaaacaac tcagcaatgc tgagattgtt gctaccgttc gggaaagcct tagcaactac
     1021 cgccccaaat cagtcagacg tgttttcatt ccaaaagctg ggagcgataa aatgcgtcca
     1081 ttggggattc cgtgtatctg ggacaggctg gtacaacaat gtatcctgca agtcctagag
     1141 ccgatatgcg aaccaaagtt ccataatcat tcctatggat tccgtgcgaa tcgaagtgcc
     1201 caccatgcag tcagcagagt aacgacactg ataaatttat caaaatatca ttactgtgtt
     1261 gacgtagaca ttaaaggatt tttcgataat gtcaatcatg gaaaactatt aaaacaaatc
     1321 tggacacttg gtattcggga taaaagattg atttgcatta tcagcaaaat gctgaaagcc
     1381 gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac aaggcggtct gttaagtcca
     1441 ctgctctccc tcatagtcct taatgaatta gactggtggg taagcagtca gtgggaaaca
     1501 ttccagccga agaaccgcag taaaaatggg tggcttcaat acgcaaagaa atataccaaa
     1561 ctgaaaagcg gttttattgt acgttacgct gatgatttta aaatcatgtg ttcaacctat
     1621 ggagaagcac aaagatttta ccacagtacc gtagattttc ttaataaaag gctgaaatta
     1681 gaaatcagtc cagagaaatc aaaggtagta aatctgaaga aaaattcatc agattttctg
     1741 gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac acggttatgt tgccaaaaca
     1801 gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc taaagttaaa ggtaaaagat
     1861 atagccagac atacgactgg cttcaatatc agcagatata atctgacagt tatcggtatg
     1921 cagaattact actgcatagc aactaacgta tataacaacc tgacagaagt aagttacgct
     1981 ctcttaccga ccatacgaat ccgtttaagg aacattgcaa aatcagttcc atttgaaagt
     2041 acaagttcag aatttcagag caggacaaaa ggaatcagac caaaaacaaa aatagtgatg
     2101 attgcggata atccgctatt gccgattcaa ggagtacaac acaaaaatcc tatgaatttc
     2161 tcgcaagata tttgtaattt tacaaagcaa gggagaaaca aggtgcatga ggacgttgta
     2221 gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg aaaaccctgc cgatagcgta
     2281 gagtttaacg acaatcgtat ttcggcatat atcgcacaac aaggcaactg ctatgtaatg
     2341 aatcgtcgag gaactccgtc cactctgata tgtattcaca aatcagacaa aggagataac
     2401 cttgacagat attctaatct ggcatttgtg gaaattccca ttgcaaaggc aatcctaaca
     2461 gaatcagcag acgaagcaaa atcactgtta aaaggttatg ttcttaatag tcagcagaag
     2521 aaaaaactga acagaatacg gacgaattac ggataccaga caataactgg gaaataactt
     2581 ataaatctat ttggatatga tggaacgccg tatgaagcga aagtttcatg tacggtgtga
     2641 agcgggggaa aacttggaga taacatc
//
LOCUS       DAFGFZ010000195         2650 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000195 DAFGFZ010000000
VERSION     DAFGFZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.199"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(1202..1867)
                     /locus_tag="LR726_002907"
     CDS             complement(1202..1867)
                     /locus_tag="LR726_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6217920.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1993..>2650
                     /locus_tag="LR726_002908"
     CDS             1993..>2650
                     /locus_tag="LR726_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL6217921.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     M"
BASE COUNT          852 a          416 c          473 g          909 t
ORIGIN      
        1 tgaccagtct attttacatt ctaatatatt catatgagct aattttatga ttaaatggta
       61 agtgtttcct tgtttgtttt agtttaaact agtgtgtaat ttaaaaatct atatggaagg
      121 gtctgtgggg taatacttaa ataaaggcta ggaacctgat aacatcaagg ttcctagcct
      181 ttttataata aaatgaagcg aaaataattt cttttaaaga gccgagatta tcagctatat
      241 aagagtgaat ttgatgcaaa aaacagagcc gaaacagtct tggtgttcgt agctctgctt
      301 atatgtttcg aatttaagac aaagagttgg caaaagatgt tgataaggaa gataagataa
      361 ctgtgttata taaactaggt aactaactgt aacaagctaa ttttaagaga ttcttattag
      421 aagacgttta ggaatataac taagaaaatg attatacagg taattgtcat ttcaataatg
      481 ttttctaatt ttcgcgtctt tgttttaaga aaaaggccag aatcagttgg aaaaaagttc
      541 gtacgatagt gaagaggggc aaaatttcct actaatattc ccactaacaa gagataaaac
      601 ggccaggttg gtgtgattcc tctataaaaa aatgcccatg ctaccgaaat cccatagatt
      661 aacaactgaa caatttttac aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct
      721 ccttgtatcg tttatttgtt tcattttatc attatgatag gcgcttgaac aatccatttt
      781 gtattttatt gagtactctt ggataaacta ccagcgatta tcatatttat ttatgttact
      841 gtaagccatc atataaaagt aaatgagcag atcatagaaa tataaaaaag aactaaaatt
      901 gatctaaatt atgtgcagct tgactacgta ccaatctata ttacttacta gattggtcgt
      961 taatctattc ggatcgaatg agcaaaatat ttttgtgaat gtccatcctt gattatgatt
     1021 ccaaaaagaa tcattgggag gctgtggcgt aaatattata ctataagcca gaaataccta
     1081 tcattctgaa cttttttatt taggaacttt catctcaagg ctttttcaat taatttgtac
     1141 ccctgttttt tggataagat catgtgcatt gattttttct atctctacgt tcatctccta
     1201 cttaatgcct atctggctcc tgagggcttc tttttaaggc gtcgtcatag tcttggctga
     1261 ggatttcttg gtaatctgag ggaatggctt cccaagtgag caggcgaacg ttcatgacgt
     1321 gttttccttt gtgtttgatt acagcatggt ttttctcttt ttctacctct ccagcgtgaa
     1381 aagacagcca tgtaccatgg ccaaagatat cattacatac ccaacgttcg atggaattgt
     1441 ccacgtatcc tacagggagt gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc
     1501 gatcgatcgt ttcatttttg tattgataat tgataagaaa aagatagacc aaacaaaaga
     1561 cgagcagaaa gaaagatagt gtctttttga gtgagaaggg ttgttttgat gaataagatt
     1621 tcttgttaac aagaggttca tctggcaatg ttggttctat tgattcggta gctgattcaa
     1681 tagaaggaaa atcaatcggc agacctaaag tatgatgtaa aagtgacagc atttcaaggt
     1741 ctggcattgt acgagaattt tcccaactag aaatcgtttt atcggaaaca ccaatttttt
     1801 cgcctaattc tttttgcgta agcttacaat ttctgcggta agttcgtatt ttttctccgg
     1861 tgttcatctt cttctccttt cgattctaca gtatatagat ttattctaca tgacctatac
     1921 acttgataga tacgtttttt tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag
     1981 taagggagag tggtgataat caaaaaaaag ttcacagttt tactcacaat catagtgacc
     2041 ggtatcatga gtggttttgc cttggttagt ataaataaac aggtactcta tcggacaaat
     2101 gaatttttcc atcaaacaga tacttatact ccgattcgat tgccacaaca ttttattggg
     2161 caagaagaat atgagcagct aatcatggta tttgaaaatg tctcatcaga aactgagctt
     2221 tcttatatga aaaaaacagt aaatgaatcc aatgaagtag atacatcttg gaacttcaag
     2281 atacagccaa agcttgaaat gacattgtat tatcaagggg atgtcaaatc ttctcacgag
     2341 aaggtaccct attctaatac ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata
     2401 aaagattttg aaggtgaatt tttcttgatg acgagagaaa aagaagccta tgaacggttc
     2461 atttcactat tcactgatcg attcaatcaa gcatttcaca ctgattatgc cactaatatt
     2521 tttgaagact ttgaaaatag ctattcgtat tcggttattt caaatacaga cgtatataat
     2581 tatcgagtgt atttaactat tggattgatt cttttattcc ttttgttatc atcctttttc
     2641 ttattgatga
//
LOCUS       DAFGFZ010000196         2590 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000196 DAFGFZ010000000
VERSION     DAFGFZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.200"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            257..532
                     /locus_tag="LR726_002909"
     CDS             257..532
                     /locus_tag="LR726_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217922.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..>2590
                     /locus_tag="LR726_002910"
     CDS             545..>2590
                     /locus_tag="LR726_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL6217923.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     "
BASE COUNT          778 a          407 c          562 g          843 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga
//
LOCUS       DAFGFZ010000197         2581 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000197 DAFGFZ010000000
VERSION     DAFGFZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.201"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(173..739)
                     /locus_tag="LR726_002911"
     CDS             complement(173..739)
                     /locus_tag="LR726_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL6217924.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(739..1695)
                     /locus_tag="LR726_002912"
     CDS             complement(739..1695)
                     /locus_tag="LR726_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL6217925.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1695..2351)
                     /locus_tag="LR726_002913"
     CDS             complement(1695..2351)
                     /locus_tag="LR726_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL6217926.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          790 a          531 c          478 g          782 t
ORIGIN      
        1 aataagaaca gccaatcaaa aacagcttct catgtttttg atttggttgg acttattacc
       61 gcagatataa gcttttgagc accgtgaata aagaggttat gaaatcaacg ctctaacctg
      121 agtattagag aaaaaaacgc aaaacatatc atttatgttt tgcgtttttt atttatcttt
      181 ttttctcaat acaatataaa ataggcggct ggttcttttg gttgataaac cgataattca
      241 gcacattata tgtttcctgt ggcagtttgg aacaaaaaga atcaaccata tccagttctt
      301 tttctccacc ttcatgacca tagtagacga caaggatcaa tcgtccgcga ggcacaagtc
      361 gtttcaatat ttcttccata gctgtgcgtg tagtctctgg catagtgatg attgctttat
      421 cactttttgg gagataccct agattgaaaa tccctgcttt gagattttgt tgttcagcta
      481 tcacttctcc taacgtttcg tggccttgta gaaacaatga gacacgttct tctaactgac
      541 gctgttctag cctttcacga gtagcatcga ttgcttgttg ctggatatca aaactataga
      601 catgaccagt ttttccgatg tgttctgcta gaaaaaccgt atcgtggcca ttgcccatcg
      661 ttgcatctac gacgtgatcc cctggttgca agatttcttg cagtaatgta tggctgaaat
      721 gcaaagcagt ttgcaacatc agacgctcac ctttttccgt acatcatatt ttccttggaa
      781 gctatctctg cgaagaagtt cttcatcaat tgcattcaag acttcccatt ttttcaagct
      841 ccacattggt ccaatcaagg aatcccacgg cgcatcccct gtcaaacgat ggatgatgat
      901 ctcttttggt atcatttcca gctggtcgca aatgacattg acatagtcag gacggctcat
      961 taattgaagg cgaccttcat gatagtcccg caacattctc gttttgcgca tcagatgcat
     1021 caaatggagc ttgattcctt ggatatctga atctaaaatg gtgcgtctga cattttcttt
     1081 catcatttct aaactttcgc ctggtaatcc gttgatcaaa tgcgtacaaa cacggatatt
     1141 atgtttgcgt aatttagcca cgccatctaa ataagtttga taatcatgtg ctcggttgat
     1201 cagttttgaa gtttcttcaa aagttgtttg caatccaagt tctacccata aatacatccg
     1261 ctcgttcagt tcagcaaggt aatcaactac ttcatctggc agacaatccg gtcgtgtacc
     1321 gactgataat cctacgacac ctggtaaatt gactacttgc tcaaatcgtt ctttgattat
     1381 ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa acgatgtatt gttggacatg
     1441 aggccatttt tgatgcatca tgtcgatttc tttctggaat tggattggaa gagggtcttc
     1501 aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa aaagtacaac cgccatgggc
     1561 aacagtccca tctcgattcg gacagtcaaa tcctccatcg atcggcacct tgaaaatttt
     1621 cccgccaaac tcatttcgta aagcgtaatt ccaagaatga tatcttttat ttccttctga
     1681 ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt tatctatttg ttttcttttt
     1741 tctctataca ctggataagt caggcacagc caagaaagac tgagtaagta gccgccgatg
     1801 atatcagtag gaaaatgaac gcccagataa atgcggctta tcccgacact tactataata
     1861 aacatcatca gcaattgaag caatgtgcgt aaggttcttt tttgaacaaa agctggaagc
     1921 agaaatagta aagaaccgta aagaatcata ctgcctgctg catgtccact agggaaactg
     1981 taacttgttt caactactaa gtgcgccaat gaaggacgct ctcgcatgaa aaatagtttg
     2041 atcaccgggt tgatgattcc agcaattaca ataaatccag aaacgaacca aaatgtttct
     2101 gccctttttt tcttatacca catccataaa gccgcacaag caaataaaat gatgacggta
     2161 agaggattac cgaatttagt cataaagagt tggtagctgt tcagatcagg ataagaagct
     2221 ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc acgacagatg gaactttacg
     2281 atccagccca aaatgaaaaa caggacaaga aagaagctac cagccacctg aaatgatgtt
     2341 ttatatttca tacaattcct cctttgaagc aatgacttat tatagcattt ttggtccagc
     2401 gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct ctttgaataa ccaatattct
     2461 tataaaaaaa ataactgtaa tcggaaagac attttttggt aatgcaaatg aaatgttagg
     2521 gaaataaaat cagtgttttt cgaaatcctt ttaaacaaga acgtagacgt aggaagaact
     2581 t
//
LOCUS       DAFGFZ010000198         2564 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000198 DAFGFZ010000000
VERSION     DAFGFZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.202"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            90..725
                     /locus_tag="LR726_002914"
     CDS             90..725
                     /locus_tag="LR726_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL6217927.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            838..1473
                     /locus_tag="LR726_002915"
     CDS             838..1473
                     /locus_tag="LR726_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217928.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1772..>2564
                     /locus_tag="LR726_002916"
     CDS             1772..>2564
                     /locus_tag="LR726_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6217929.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKI"
BASE COUNT         1014 a          380 c          436 g          734 t
ORIGIN      
        1 ggattattaa tcaagaagaa ttcgatttta agaaaaaaga attgcttggt ttataatatt
       61 caatatcaaa agagaaagag gatctattaa tggacggtat atttggtttt actggtattg
      121 ttttatttat tataggttta atcatgttaa ttgtaagatt tatcaaaaaa acaaataaga
      181 aaacacctat gattctcctt attatcggga ttatttttac taccgtaggt ttctcgcttt
      241 cctcatcaaa cgaggcaaat gacaataaca aacaggaaag ctcatcatct gatactcaaa
      301 ctactacatc taacagcagc aataaaagcc taactcaatt tgaagaattt ttagagacaa
      361 ataatcatga ctggggtgct ttcttagata gttattattc tattactcca gctactgagc
      421 aaaatacagc ttttactaaa tatattagtg gtaaaacata tacttttgaa ggaactgtta
      481 tcgaatcaat gactactaga atagccatta tagcagacaa agagtacgat gataaaagtt
      541 ggaatgatat ttctgcaaca cctaaagttt catatgtaat ttttgcgaag gacttaaaca
      601 atgctgatac ctttacaaag ggcgataaag taacttttac aggagaaatt agttcaagag
      661 gatcgaacat agaaaaatcc tatgctcaat gggatatgat taatagtaaa gtaagtaaaa
      721 aataaatgaa ccacgcccca ctaagagtta gattgtgcac aataataaaa cctatacaaa
      781 agagtgccat caggagtcac aatgcttgta aaggaaatga caaaatagga ggtaactatg
      841 gataatagca atgaaaaact aaccaaaaaa gaatatcttg aattaatagt tgaaggcggt
      901 attggagcaa ttccttatat tggtggcgtt gtgcagtcac tttattttgg gtctaaaaac
      961 gagaagcgtt tcaaacgcat cgagaaattc tacaaaaatt taaacgaatc attagaaaac
     1021 attcgcgatc aaattccaga agatgttttc aacttagaga acaaggacca actcattgga
     1081 atattcgaag ggataaatga cgaggtagaa aaatcaaagg cacaaaataa aatagatatg
     1141 tataaaaatc tttataagaa ttgtttactt cgaattaaca gcgcttcatg ggataacgag
     1201 gagtattatc ttcttgtact gaatcaatta acatcgattg agattcaatt gttagcatgc
     1261 ttaatgagtc gtggaaacga aaatttcact ggaaatatca gcatgtctgg ctattcacaa
     1321 gagctaatag atggtagctt aaatagatta tctgattttg gtctgttaga aaaaacgatt
     1381 agctccatag tattaggtga tgtcggcaaa caaaatatgg gctacaaaat atctcattta
     1441 ggaattcaat ttattaactc aactcttact taagttttct ttctaatatg acatctagac
     1501 ctatcagcaa tgcaacaata aaaaaccaga cataccaata atcaacaaat aatctaaact
     1561 ctggaagttc agttattctt ttaaaaatca ctttaatcat aataaacacc tctgattgga
     1621 tgatctaata cacatccaat tataccatta aaacgacaaa tagcacatcc ttgcgccggc
     1681 aagcaaaaag gatgtgcaaa aaataataaa acccaaacaa tgggcttctt tatagttcct
     1741 attgtatcag agaaagagag ctgattcaat tatggcaaga ttagtcaaac gtggaaatag
     1801 ttggcaatac gaaatttcat acaaaaaaga tgacggaaaa tacacgaaga taagaaaatc
     1861 aggatttaag acaaaaggag aagcaaaaga tgccgccaac gaattagaat ataacttgaa
     1921 caaaggcctt aaaggggatc gcaaaaatct attattatca gattactttg aggattggat
     1981 gcaactttat aaagaaggaa cagtatctcc tatcacttat agaaaatacg aagatacgtt
     2041 aatgaacata aagaaatata tgccagcggt attgatttct gatttagata gagtgggata
     2101 tcaacgcttt ttaaataagt atgctaaaga ccatgtaaaa tctaccgtta ttaagtttaa
     2161 taaccatatt agagcgtcgt tgaaagatgc cgtagaagaa ggattaattc cgtttgatcc
     2221 aactagaaaa gcagtaatca aaggaaaaga ttcattgaag ccaaaagaag ataaatattt
     2281 agattatgat caatttaaat ctttaatgaa actcgtagaa gaaaaccttt ctgcgcagta
     2341 ctcttctcct atgctcgtgt tagttgctgg tgccactgga atgcgatttg ctgaacttct
     2401 aggattaaca tgggaagata tcgatttcga agatcaaatc atcactatta ataaaacatg
     2461 gaattataaa ttaaatgaat ggggaaaaac aaaaaacgaa acttcaaata ggaaaatttc
     2521 cattgataaa catacgattg atctcttaaa aaagtttaaa atca
//
LOCUS       DAFGFZ010000199         2543 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000199 DAFGFZ010000000
VERSION     DAFGFZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.203"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            7..1014
                     /locus_tag="LR726_002917"
     CDS             7..1014
                     /locus_tag="LR726_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL6217930.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1026..2030
                     /gene="neuB"
                     /locus_tag="LR726_002918"
     CDS             1026..2030
                     /gene="neuB"
                     /locus_tag="LR726_002918"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBL6217931.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2027..>2543
                     /locus_tag="LR726_002919"
     CDS             2027..>2543
                     /locus_tag="LR726_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HBL6217932.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVG"
BASE COUNT          929 a          328 c          510 g          776 t
ORIGIN      
        1 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
       61 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
      121 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
      181 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
      241 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
      301 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
      361 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
      421 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
      481 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
      541 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
      601 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
      661 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
      721 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
      781 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
      841 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
      901 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
      961 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     1021 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     1081 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     1141 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     1201 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     1261 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     1321 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     1381 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     1441 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     1501 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
     1561 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
     1621 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
     1681 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
     1741 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
     1801 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
     1861 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
     1921 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
     1981 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
     2041 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
     2101 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
     2161 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
     2221 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
     2281 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
     2341 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
     2401 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
     2461 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
     2521 ttggagagtt tttcccgtcg gag
//
LOCUS       DAFGFZ010000200         2529 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000200 DAFGFZ010000000
VERSION     DAFGFZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.204"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..271)
                     /locus_tag="LR726_002920"
     CDS             complement(<1..271)
                     /locus_tag="LR726_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217933.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFIN"
     gene            complement(602..835)
                     /locus_tag="LR726_002921"
     CDS             complement(602..835)
                     /locus_tag="LR726_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL6217934.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(850..1800)
                     /locus_tag="LR726_002922"
     CDS             complement(850..1800)
                     /locus_tag="LR726_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL6217935.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1797..2519)
                     /locus_tag="LR726_002923"
     CDS             complement(1797..2519)
                     /locus_tag="LR726_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL6217936.1"
                     /translation="MSLMGDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFI
                     KSNSVIKGDTAIGITFSSFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGV
                     SVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTIL
                     IVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAF
                     LFVISFLISPKQKFLRKKERSL"
BASE COUNT          831 a          471 c          311 g          916 t
ORIGIN      
        1 aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct atatctatat
       61 tttctaagtt taagaggtat ttggtaaact cttcattata catattaata agcgcattaa
      121 tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag
      181 cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc tgaaatcctt
      241 ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata atataatctc
      301 tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct tacacaggac
      361 ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta aactaatgag
      421 aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat
      481 cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg
      541 ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct
      601 tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa
      661 atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg
      721 gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata
      781 attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta
      841 ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc
      901 atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata
      961 gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc
     1021 tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc
     1081 tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc
     1141 tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat
     1201 tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc
     1261 tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat
     1321 atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc
     1381 tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc
     1441 tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt
     1501 ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc
     1561 aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat
     1621 tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt
     1681 taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa
     1741 tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat
     1801 aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca
     1861 aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca
     1921 ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt
     1981 ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt
     2041 aatatagttc ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat
     2101 agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa
     2161 gtgattaata attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca
     2221 attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat
     2281 aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg
     2341 aaagtaatac caattgctgt atctcctttt attacactat tgctttttat aaaagtaata
     2401 atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata
     2461 taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt
     2521 cctcttaaa
//
LOCUS       DAFGFZ010000201         2501 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000201 DAFGFZ010000000
VERSION     DAFGFZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.205"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            100..417
                     /locus_tag="LR726_002924"
     CDS             100..417
                     /locus_tag="LR726_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBL6217937.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            414..575
                     /locus_tag="LR726_002925"
     CDS             414..575
                     /locus_tag="LR726_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL6217938.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            578..877
                     /locus_tag="LR726_002926"
     CDS             578..877
                     /locus_tag="LR726_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217939.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            877..1191
                     /locus_tag="LR726_002927"
     CDS             877..1191
                     /locus_tag="LR726_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL6217940.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1184..1714
                     /locus_tag="LR726_002928"
     CDS             1184..1714
                     /locus_tag="LR726_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL6217941.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1711..1980
                     /locus_tag="LR726_002929"
     CDS             1711..1980
                     /locus_tag="LR726_002929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217942.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1910..2113
                     /locus_tag="LR726_002930"
     CDS             1910..2113
                     /locus_tag="LR726_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL6217943.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2110..2397
                     /locus_tag="LR726_002931"
     CDS             2110..2397
                     /locus_tag="LR726_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217944.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT          935 a          371 c          579 g          616 t
ORIGIN      
        1 ataaaaaaga ataggttttt gggtctgacc ctccaaactc tccaccaaaa tcttcctaaa
       61 tgctttgata ccaacgatta aacagtctgg agggttacat tgaaatcaga acatcaaata
      121 caagacgaga tcagaattgc cttatcaaaa catggttgca cgatttttcg atccaatgcg
      181 ggacaagtga caacgatcga agggcggcaa ttttacggga tgccaaaagg gttcccagat
      241 ctatgtggac atcgtaattc tgatgggaaa gcaatttata tagaagtaaa aaatgaacgc
      301 ggtaagttgc gtgaagatca aaaaaggttt gctgaatttt taaacggtca acctgtacta
      361 tacggtgttg cacgatcagt ggaagaagca ttgaaaattg tggaggaatc atcatgacaa
      421 cagaagaagt gattcaaatg cgtattcgaa gccttcagcg tgagattgac gatctggaac
      481 ggacaaaggc agtgatggtc aatgaaacgg cgaggaaggc aatcgatttg cacatagaga
      541 atttaagaag ggaaatccat cgattggagg aatgagcgtg gataagaaag cagcaatgaa
      601 acgaatcatc gaactgacac attctgagaa ttggcaagaa gacaaagaaa tagttgcaga
      661 agtccaaaag ctcggtaaat caatgtggag tgaaaaaacc aaacggagaa caccgagaaa
      721 aattgcaatc tggcatggtg accgaattct agtaacaggt actgctgaac agttatctga
      781 aattactgga ttaagcaaaa acattatctg ggatagagct aaacgcgaga acgttgattc
      841 gaaaggtcgt caatttaaac attgggagaa gaaataatgg aggaactaat cacaaaagta
      901 gagcagtggg caaaagataa gggattggat caagctgatt ccagcaagca aatgttaaaa
      961 acgatcgaag agattgggga agttgccgct tctctagcta gaaaagatga atatggttta
     1021 agagacggaa ttggagacgt agtagtaacc ttgattattt tagctatgca aaatgatatg
     1081 gatttgtacg agtgtctgaa ccaagcatac aacgaaatca aaggacgcac aggaaaaatg
     1141 gtagatggtg tattcgtgaa gtcgagtgat ttggaggaca gcgatgaata aaaaagtatt
     1201 aattgataaa caagcattga ttgatgaatt aatgaaaata cctggtgtgg gatctaatag
     1261 tgacgcttta gaaacgatta aacgtttccc atcgtatgaa ccgcagaagc cagttgtgcc
     1321 taagtttgtg gcggaactac tcgactatta tcgtcagtca acagatgttg atttattagc
     1381 tttattgata actttccatg attggtatta tcgcaaaact aaagacggtg agcatgaaga
     1441 agcaattgat tggctagttg atcatcctga aatttatatg cgtgcatggc tgtttggcta
     1501 cgagctcgag aaagagccgt tgtattgcgt aataatcaat ggtagatatc ttgtcaaagt
     1561 attcagcaat acaaacgttg tcatattagt tccagcagat gaattttcac agtacgttac
     1621 tcaaaaattt caattaactg aaaaacaaat caaagcaatt gatgaaagat actggccgtt
     1681 tgctgtgcca gtggatgagg tggtagaagg atgagcgacc catacattac taaattagaa
     1741 aagttgtccg aagattttga gaagaataaa aataaactaa ctaaagataa acttatagaa
     1801 ctggttaggt tggttatcag aggagctatt acaagagaaa aggcgcagca aaacatagaa
     1861 atttttgctc gtagtgtccg agatggatta gagaagaaag ttaaacaaaa tgaacaacag
     1921 acaccgcaga ataacaaaac taagaaaacg ggaactgaat gtactaaaga caaagtttga
     1981 aaaagaatat ggaatttcag cagaagaaac atataaagtg gcaagtcagt gtgttgctga
     2041 tgcgagcggc gctattcgta agtttggaat ttcgatatta agtgatgatc gtaaatggga
     2101 ggaaaaagaa tgaaactaaa agacggattt tactccagca gtcatggtat cggcggttta
     2161 atgctagata tgccgacaaa gaaccctaaa acacgtgaga aaccaaaatt caaagtcggt
     2221 gacatggttc gctgtgaagc agaaggattc atctatccat tttgtggata tgtagaacat
     2281 ctctataatc actcagcaat cattcgtatt gaaaacacga tggaatgtga caagtggtta
     2341 gcgaaaagca aagagaattt agcagtagcg agattggtgg atatggaggt tatataaaca
     2401 gcgaaaaaat gtacttctaa gagaaaaatt tattttttat aaaaatagga aagtatcatt
     2461 atgaaaaaga ccacagagat aaataaacct accacataaa a
//
LOCUS       DAFGFZ010000202         2495 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000202 DAFGFZ010000000
VERSION     DAFGFZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.206"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            184..420
                     /locus_tag="LR726_002932"
     CDS             184..420
                     /locus_tag="LR726_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBL6217945.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            439..654
                     /locus_tag="LR726_002933"
     CDS             439..654
                     /locus_tag="LR726_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBL6217946.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1357..2109)
                     /locus_tag="LR726_002934"
     CDS             complement(1357..2109)
                     /locus_tag="LR726_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6217947.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2124..>2495)
                     /locus_tag="LR726_002935"
     CDS             complement(2124..>2495)
                     /locus_tag="LR726_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL6217948.1"
                     /translation="NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDR
                     ELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNP
                     NMFVNYQNDPYQFTTQVILLF"
BASE COUNT          815 a          399 c          411 g          870 t
ORIGIN      
        1 gtctcataat tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg
       61 aatcaaattt attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga
      121 tttttactgt taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga
      181 agcgtgttta aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt
      241 ggtggaggca ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg
      301 gcagtaggtg gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga
      361 gggttatatg gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa
      421 aaaaggagaa taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca
      481 gaagagttga aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct
      541 ggggcaacag caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt
      601 ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt
      661 aaaaaatatc aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat
      721 cttttcaaaa gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat
      781 aattatatta aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg
      841 gtagagatat ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat
      901 ttcggccgtt acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc
      961 tgcaagttaa atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca
     1021 taggattgca cttgacagga caaatttttt aactaaaaaa tgaaaaacac tttctaaaaa
     1081 ttagtagaat ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt
     1141 tatggtactt aataaaaaca agaaaagaac tattcatttg gccctttttt ggttccagac
     1201 catagaaata ttcctccagt aactaatatt gctgttatgc acgtaactaa taacagctat
     1261 aaaaattggt acaactttaa taggattcag aaaaatttgc actagtgaag gaatctattt
     1321 tattagaaaa atgcaatctc cctaaagaaa aaaccactaa acagtgcttt ctttcaactt
     1381 ctccttaatt ttttgtgctt tccctaaagt aaagtttctg gccaactctg aatcaaaata
     1441 tagtgaacgt tttttaaaat ctatttcttt aacgttaaag atatttacta aacacgctcg
     1501 actaattctt gttaacatag gatatttctt ttcaagctca ctaattcttc cataaaattc
     1561 gtactgccca tctttagtac acaatgataa tctatgtggt aattttgaag cttctacaaa
     1621 ataaatgtca tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg
     1681 attcgtaacc tgttttgttg catctatcct ttcttgcgct aataacaata actcaacaat
     1741 ctcgtttcga tactcatcta gtgtttgatc ttttgttaca aatcctaacg tttcaactct
     1801 tctttttatt gttacgggta acatctcatc atgagtagtt gtaaaaatga tttttgcttg
     1861 aacatcatat tttctgatta cttctgctaa ttctataccg ttaacttcat gatttaaatc
     1921 aatatctaag aaatatattc cttgctttgg tcggaattgt ttgaggtatt ttttaacttc
     1981 taacggactt tgtgttttca atactatctt aaatacttta tcgtgaaaaa gaatgaaatt
     2041 atcaataatt cgttctaact gatttaattg tataatttga tcttcacata atattattgg
     2101 ataactcata aatcctcctt ccctcaaaac aacaaaataa cttgagtagt aaattgatac
     2161 ggatcgtttt gatagttcac aaacatattg ggattctttt tattgatttc ctctatacta
     2221 gataaaccta acccttgatg tccctttttt gttgttattc cttttttcat tagagtatct
     2281 atagagagta gtatctctct acaactattc gatatcgaaa tttctatctg tttatcatct
     2341 cggtaaatag ctagagacag ttctctatct tgactctcta tagatgcttc aattgcatta
     2401 tcgagaataa ttccaagcac cctaatacag tcaaatattg gaacaggaac ttcaataata
     2461 gtacttggac attcgaattt acaacaaatc ttatt
//
LOCUS       DAFGFZ010000203         2453 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000203 DAFGFZ010000000
VERSION     DAFGFZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.208"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..435
                     /locus_tag="LR726_002936"
     CDS             <1..435
                     /locus_tag="LR726_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL6217949.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="LR726_002937"
     tRNA            complement(759..842)
                     /locus_tag="LR726_002937"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="LR726_002938"
     CDS             1370..1870
                     /locus_tag="LR726_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6217950.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="LR726_002939"
     CDS             complement(2060..2404)
                     /locus_tag="LR726_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL6217951.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          764 a          474 c          405 g          810 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tag
//
LOCUS       DAFGFZ010000204         2400 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000204 DAFGFZ010000000
VERSION     DAFGFZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.209"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            207..2069
                     /locus_tag="LR726_002940"
     CDS             207..2069
                     /locus_tag="LR726_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL6217952.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="LR726_002941"
     CDS             2069..>2400
                     /locus_tag="LR726_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL6217953.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAFGFZ010000205         2398 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000205 DAFGFZ010000000
VERSION     DAFGFZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.210"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..359)
                     /locus_tag="LR726_002942"
     CDS             complement(<1..359)
                     /locus_tag="LR726_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL6217954.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNI"
     gene            complement(778..996)
                     /locus_tag="LR726_002943"
                     /pseudo
     CDS             complement(778..996)
                     /locus_tag="LR726_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1027..2241)
                     /locus_tag="LR726_002944"
     CDS             complement(1027..2241)
                     /locus_tag="LR726_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL6217955.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT          747 a          533 c          384 g          734 t
ORIGIN      
        1 tcaatattta aatctacttc tgtgatatac attttatcta ctatttgcaa agcttcctta
       61 aatagtccat atccaccaga tatgtataca tctcgtcctt tagccaataa taatgcatct
      121 tctaatgatt taactgaaac taaattatct ccttgatact ctgttgtggt ggaaacaaca
      181 atattcattc tattaggcaa cggatgaccg atttcttcat aagactttcg ccccataata
      241 accacattac ccgttgttaa ctctctaaat tgcttttgtt ctccctttat tttccatggt
      301 atattaccat tcttgcctat aacattattc tttgacctcg caacaatcaa acctatcatc
      361 tatttcatcc tccataatat gatgtcgtac ctgtgattcc atcaagtgca ccacatctta
      421 acaaactcaa aattatctcg aactaaccag ctcgacaaat tccttttttg tcttcatctg
      481 cattatatca tattgttata agcattgcca tttcttttct gcttttgaaa agttacttat
      541 cagcctatgt tttgtaaagg gtgatattca gcatttctca cgcaacaagc ccttgacaaa
      601 ccatagacta ataagtttta aggtaatcaa gcagaaatga aatagtctgc tgtttcacgg
      661 caacaaaaaa gagataccaa cacacttatg tagtatctct tagtctagta gcaatattct
      721 tttgtttgca agcaggtaaa catgggctgt ttcttaatat ctttttgttt gggaaacacc
      781 tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag ggataaacca
      841 tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg cgaatgtggg
      901 atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa agaacgttaa
      961 tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt tctatttgaa
     1021 tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc
     1081 ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg ttgtcagcac
     1141 ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga agtaccagct
     1201 gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt
     1261 gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt gtttcttttt
     1321 acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca gcatggcact
     1381 ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa cggatcctgt
     1441 tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca gccaagtaat
     1501 catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt gttcatctgc
     1561 tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat
     1621 agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct ttctttgacc
     1681 aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga ctgctccacc
     1741 agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc agtggcaaac
     1801 agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt ttaaattcaa
     1861 tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca tcagttggta
     1921 aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga agcttgtaac
     1981 atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat cccgtccaaa
     2041 ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg agagaaaaga
     2101 ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa aaaaagctag
     2161 accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg tatctcctgt
     2221 attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag tataaaagaa
     2281 ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat aaaagacaga
     2341 caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa taaagaca
//
LOCUS       DAFGFZ010000206         2397 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000206 DAFGFZ010000000
VERSION     DAFGFZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.211"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..586
                     /locus_tag="LR726_002945"
     CDS             <1..586
                     /locus_tag="LR726_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HBL6217956.1"
                     /translation="DIKEKYHLKFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQ
                     SQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSG
                     IIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKN
                     IYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            559..1236
                     /locus_tag="LR726_002946"
     CDS             559..1236
                     /locus_tag="LR726_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL6217957.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1226..2368
                     /locus_tag="LR726_002947"
     CDS             1226..2368
                     /locus_tag="LR726_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBL6217958.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
BASE COUNT          904 a          315 c          465 g          713 t
ORIGIN      
        1 agatataaaa gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt tgcaagtatt
       61 tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa attttagctt tgatcaattc
      121 aatggaacaa caatctcaac caattatttg cattttacct aatagtgatg ctggtagcga
      181 aattatcaga aaaatgtacc agaaatattt aaaaacggat attatttata ttacgaattt
      241 agagccggag gaatacctga ctatattaaa agagtgcaaa tttatgatcg gaaattcttc
      301 ttctgggata attgaagctg catcattcaa aataccagta ataaatttag gaaccagaca
      361 aaatggacga gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa atgaaattga
      421 aactgctttg aataaaatac aagatcctca gtttataaaa agcttgaaaa atgttaagaa
      481 catctacggt gacggcaaca gttctagaag aataacaaaa acattgaatc aaatattgaa
      541 tgatggggaa gatattaagt ggattcaaaa acaaatagct tattaaaata tactagtgat
      601 ccatacatta ttattggggc tggcggacac gcaaaagtca ttattgaaac cttaagatta
      661 gaaaataagt taatatatgg attttttgat gataagcaaa ataaaatgca agatctgaaa
      721 tatcttggga aaataaacga attgaatagc tgggcggata gcaaaaaaaa aatctataaa
      781 tttattgtag ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg
      841 ccaagtgagc aatatggaat agctgttcat ccaagtgcta tagtggcaaa agaaatttca
      901 attggttatg gaactgttat ttttgcgaat gctgtaatta acccagatgc tgtaattgga
      961 gagcatgcta taataaacac aggtagcatt gttgagcatg attgtagaat taataactat
     1021 gttcatttat ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt aggaactcaa
     1081 ataggaattg gttctcaatg tatccagatg aagaaaattg gatcttggtg tatgataggc
     1141 gcagggagca ctattgttaa ggatattccc tcacattcct tagcttatgg caatccagct
     1201 aaaattaaga aagaaggaat taattttgaa ttctagaatt ttattatcaa gtcctcatat
     1261 gggaggaaca gaagaaaaat atgttgctaa ggcttttaaa gataattgga tagcgccatt
     1321 aggtgaaaat gtggataaat ttgaagaaag tattacaaaa tatactggtt ctaagggcgg
     1381 agcttctgcg ttaagttcag gtacagctgc catacatctt gccttaagac tgcttaatgt
     1441 tggaaaaaac gatattgtct ttgctccttc atttactttt atagctacag taaatcctat
     1501 tttatatcaa ggggctgtac cagtgttcat tgactcagaa cctaatacat ggaatatgag
     1561 ccctgttgca ctagagagcg cattagaaga agctgaaaga aacaaccaat tacctaaggc
     1621 aatcatcgta gtgaatttat atggtcaaat ggctgattat gaacgaattt tagctattgc
     1681 aaaccaattt aatgttccag tgatagaaga tgcagcggaa tcgttaggtt ctacttataa
     1741 gggaatttcg tcaggcttat ttgggacaat tgggatctat tcatttaacg gtaataagat
     1801 catcacaacc tcaggtggag gagctctagt gtctagtgat aaaaaatatg ttgagcattc
     1861 aagattttta gcaacacaag caagagatca agcaccgtat tatcaacata gcgaaatagg
     1921 ttataattac cgtctaagca atatttctgc cggtatcgga cgtggacaaa tggaagtatt
     1981 gaatgataga gtagctaaac gaagagaaat tttcaatcga tataaggaag ctttttctgg
     2041 aattgataga attgaaatga tgccagaagt ggatggcatg gtgccaaatc gttggttgtc
     2101 tactttaacg atcaatgatt ataattctat tgatcaagta gttaacgtaa taaataaact
     2161 aaatgaaaaa aacattgaaa caagagcttt gtggaaacca ctacatttgc aaccagtatt
     2221 tcaaggggta aaaatgtatt accatgataa taatcttagt gtgtcagaaa agttatttaa
     2281 tagcggctta tgcttacctt ctggaagtaa tatgacagat agtgaacaag aatttgttat
     2341 tgatgagttt aaaaaaatat tcaagtaggt gtgatatttt atgaacatag gaattat
//
LOCUS       DAFGFZ010000207         2394 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000207 DAFGFZ010000000
VERSION     DAFGFZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.212"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            22..1657
                     /locus_tag="LR726_002948"
                     /pseudo
     CDS             22..1657
                     /locus_tag="LR726_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1911..2225
                     /locus_tag="LR726_002949"
                     /pseudo
     CDS             1911..2225
                     /locus_tag="LR726_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          804 a          358 c          431 g          801 t
ORIGIN      
        1 aaaggagtat ataattaagt aatgcaagta aagaaaaaaa taatattgtc cattctccta
       61 gcaattattg ctggacttta ttcaatcaat tattttagaa atgttcatac tatttctaca
      121 tcgggggata ttgccttcca ttttgctaga gtcaaagggt tatcttcaat tttttctggt
      181 ccaatcaatt ttacgacgtt taatcattat gggagcggag tgaattattt ttatccctac
      241 ttgacctttt ttccggcagt cattttttat tggataagta ataatttaat cgttagttat
      301 atcttatatg tttggctatt gaatgtttgt actatcatgc tgatgtttca ttatggacta
      361 aaatttttaa aaagaatcga tgcggctttt atatttagct gtttatatac attttatggg
      421 taccgtacga ttgatattta tcatcgttct gctattgctg aagcgattgc acttacggtt
      481 ataccgatag ttatgtatta tgcttacgca ctgatttatg agaaaaagcc atgtgcaatt
      541 ccattagcag tcagtatgag cttactcatt tatacacatg ttctttccac ttttatgagt
      601 gtaatagccg ttggcttact agtcttaggc ggctttgtag taagtgaaac aagaaaaaaa
      661 gaaagcatcc ggttgattgt ggagttgtca aaggcagtag gaatgacgat tcttttaaca
      721 gcatactttt ggtatccgat gttccgacaa atcatggttc aatcgattaa tgagccagat
      781 aaaacgacct tacaacacca agcattaaat gtctcggata gtttgattgg tgcgatgaat
      841 aatgacctga caacctttac ggtaggaatc attggtatta tttctttgat ctgtccactc
      901 ttctttttta aaggatttaa tcgcaaagaa aaagtgattt accttagtgc ggtagtctct
      961 tggctcttat ccaccaaatt ttttccatgg tctttattac aaaatacacc cattcaaatg
     1021 attcaatttc cttggcgaat cttaggcttt caagtactgt ttggctcgtt gattttatcg
     1081 ttcgttttca tgaagagcac atcagctaag caaagaaaag tagttaatat aacggcaatt
     1141 gttttattac tgttggctgt atctgctgca acaaaagcaa actatgaaca taaagttgtc
     1201 actcagagag atcacttggt aatgaatcaa gaaaatctta ttcgttacac gacactcctt
     1261 cccggaggac tttatgacta tgcacccacg gaagcattac aatacaaaga acatttgcag
     1321 aatcatgaag taagaattgg caaggaatgg catactatgc cttatcaagt gatggattcg
     1381 aaaatagtct atacagtaca tgaatctaaa ggacaaaaaa taacgcttcc tgtatttgat
     1441 tattggggaa cagtcgcaaa agtaaatgga caaaaaagag agattgataa taggactagt
     1501 gtcgattgaa ggaacacaag gagaaacaat cattgaagtt tcttccattt atcctccaac
     1561 tatgaagtta gcttttttaa ttagtttatg tagttacctc tttcttttaa ttagttttct
     1621 tatcaggaag ttacaacata taaaaataat gatctgatta catttttttc aatattagct
     1681 tactagagaa taagaaaaag taagtaataa accaaattta aactttatat tataataatg
     1741 aagcttaatc ttctttatag agaattgggc tatttttaat ggcaaagagg ggaagaagat
     1801 gtttcaaaac aaaggacaac gctatgttgt aaaaggagta aaggaatctt actggaatca
     1861 aaaatcttta ttgagaaaat tatctttgaa tttaagcaca aagaaatgaa ggtacactag
     1921 atagcaatga agatatcttt gtcattaaac gtcgctattt tacacaagta agtgatgatt
     1981 tgattcatgt cttttccaat gagtggtttg tttctgagaa aaaattacat gcttctaatc
     2041 tgcaatatat gccaggtgaa gatccaattc ctaatatgaa agcaattatc aacagtaagg
     2101 attacgaagg atataaagcc aaacatccag aagcgaaacc atttaaatac ccacaagaaa
     2161 tgaagcgagc atggcgtaaa atgttagatg atgagttgat tccacttgaa aatgaattga
     2221 ggtaaacgag aagagttaag aaaattgtac gattcaaaga tagtagtaaa gtggaagtgt
     2281 agaaaatagg gatagatgtc tttttacttg ttcacatttt ataacggagt atttaagaaa
     2341 gctattgaat aatgattctt ccatcttttt cattcattta ccggttctgt tgca
//
LOCUS       DAFGFZ010000208         2394 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000208 DAFGFZ010000000
VERSION     DAFGFZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.213"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..89
                     /locus_tag="LR726_002950"
     CDS             <1..89
                     /locus_tag="LR726_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL6217959.1"
                     /translation="GFHSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            230..1843
                     /locus_tag="LR726_002951"
     CDS             230..1843
                     /locus_tag="LR726_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL6217960.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          752 a          424 c          456 g          762 t
ORIGIN      
        1 taggatttca ttcgatccat catttttctt cagcttttaa aaaatataca gggttatccc
       61 cttcaaagta ttatctatcc ttaaaataga aaaaaatttt taggttttat ccacaaatta
      121 tttctctata ttttctgtct attcaaaata aacaataatt ttccggcaca attatttgct
      181 gttttttacc ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt
      241 tgtcattggc ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga
      301 agcgcacgaa atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt
      361 gccttattat ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga
      421 aatattgaaa acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga
      481 tccttctact aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga
      541 ttacgataag ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt
      601 ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc
      661 gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct
      721 ggaattagct gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc
      781 acaagtcgct ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa
      841 agaaggatta gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga
      901 aatagtatta tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt
      961 ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat
     1021 cattgttgat gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat
     1081 cgaaacgact agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa
     1141 tcgtcaagga catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata
     1201 tattggtgca ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga
     1261 acatgaatta aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga
     1321 ccatgcctat ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga
     1381 tagcggtcgt attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg
     1441 agaactttct gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt
     1501 gccttactct ccaccgtatt ctactacccg tgacccgttg aatatagctg gttatgtcgc
     1561 aatcaatcaa atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga
     1621 tttgaaagaa gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat
     1681 ctcggcaaca aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa
     1741 agataaaaaa gtttacatta ctttcagaag aggattgaat acttatactt ctgcccgaat
     1801 cttggcaggt ttgggtatca aagcgatttt gattgaagaa taaaaaaacg accgcggtgg
     1861 gtagccgcgg tctaggagtt atttttactt gaggagtaaa aatgaaaaag tatatagggt
     1921 tgttggttgg tatgtatata gaatagaaga taaatgtgaa gaaattgtgc attatttctt
     1981 cacatttatt aggttatagt ttaagaaagt ttaaacattg cgcaatttaa ttattttagc
     2041 tgtttctttt cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg
     2101 tctctagagt aactacgcct aatattacga ggcatcatat taatctatca gcactagtac
     2161 ctaaactagc atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc
     2221 taatctcacc aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac
     2281 gacttttcta ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa
     2341 tgatccctaa tgtgtgtatt aaataattta atttttttgt tcctatttat ttaa
//
LOCUS       DAFGFZ010000209         2352 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000209 DAFGFZ010000000
VERSION     DAFGFZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.214"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..544
                     /locus_tag="LR726_002952"
     CDS             <1..544
                     /locus_tag="LR726_002952"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL6217961.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="LR726_002953"
     CDS             complement(1017..1589)
                     /locus_tag="LR726_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217962.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="LR726_002954"
     CDS             complement(1605..2210)
                     /locus_tag="LR726_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBL6217963.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          784 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gt
//
LOCUS       DAFGFZ010000210         2340 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000210 DAFGFZ010000000
VERSION     DAFGFZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.215"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            132..344
                     /locus_tag="LR726_002955"
     CDS             132..344
                     /locus_tag="LR726_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217964.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="LR726_002956"
     CDS             506..1024
                     /locus_tag="LR726_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBL6217965.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="LR726_002957"
     CDS             1031..1543
                     /locus_tag="LR726_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217966.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..>2340
                     /locus_tag="LR726_002958"
     CDS             1814..>2340
                     /locus_tag="LR726_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6217967.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          892 a          298 c          385 g          765 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
//
LOCUS       DAFGFZ010000211         2324 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000211 DAFGFZ010000000
VERSION     DAFGFZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.216"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>2324
                     /gene="esp"
                     /locus_tag="LR726_002959"
     CDS             <1..>2324
                     /gene="esp"
                     /locus_tag="LR726_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL6217968.1"
                     /translation="VSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTTE
                     LVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTGD
                     TLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFVR
                     INDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDNL
                     KTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVSA
                     KYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVPY
                     VAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFAN
                     ASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQNT
                     YGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGTG
                     HKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSPG
                     FDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSNV
                     TDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDKP
                     DVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQDG
                     SVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          844 a          370 c          474 g          636 t
ORIGIN      
        1 gttagcaaga ataataagag agtatttcta gaaaaaacaa agaaacgagt attgaagtac
       61 agtatcaaaa aattaagtgt tggggttgca tctgttttga ttggtgttgg tcttgtcttg
      121 ggaacaactg aactagttaa agcacaagat gaaatttctc caagcagccc acttgaaaca
      181 gcgataagct ccgtacaagt aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat
      241 cctggttata cgaaaaatta taacttttcg gatttacaat ttagtcctca agaactaact
      301 ggagacacac taaaaggcaa tacaattgga tttgaagttt acggaaaaca taatattgca
      361 gcatcaacta aaaactggga aatccgtctt caattagatg aacgattagc taaatatgta
      421 gaaaaaattc aggtcgatcc aaagaaagga attggatctt ctagacggac ctttgtaaga
      481 attaatgatt cgctgggtag acctacaaat atttggaagg tcaattatat tcgtgcaagt
      541 gatggattat ttgctggggc agaaacaacc gatacgcaaa ccgctcctaa tggggtaatt
      601 acgtttgaaa agagcctaga tgaaattttt aaagagatag gcatagacaa tctcaagacc
      661 gatcgtttaa tgtatcgaat ctacttggta agtcatcaag acgatgataa aattgtaccg
      721 ggtattgata gtacgggcta ctttttaaca gattcagatg atttctataa tagcttagat
      781 gtatctgaaa ataatcctga ccagtttaaa catggatcag taagtgcaaa atatgagaaa
      841 cctaatactc aaacaaaaga tggttcagga tccacaggtg ctaatggagc tataatctta
      901 gatcataagt taacgaaaaa ttataatttc tcatattcat cttccgcaaa gggcacgcct
      961 tggtatgcta attacaaaat tgatgaacga ttagttccat atgtagcggg tatacagatg
     1021 cacatggttc aggctgacaa agtaacatat gatgtttcgt ttgaatccgg aacaaaagta
     1081 gctgatttag caatcgagcg acgggaaggt catgaaaatt atggcatagg ttcaataact
     1141 aataatgatt taactaaact tatcgatttt gctaatgcaa gtccacgtcc agtcgttatt
     1201 cgatatgttt tacaattgac taagccatta gatgaaatct tagaagatat gaaagcaaca
     1261 gcacaagttg aagaaaataa accatttggt gaagatttca tctttgattc ttggttgtcg
     1321 gatacgaata aaaaattaat ccagaacact tatggaacag gttattatta tttgcaagat
     1381 attgatggtg atggaaaccc tgacgataaa gaagagagcg gagacacgaa tccatatatc
     1441 gggaaacctg aattagaaga agtatatgat gttgacacaa cagttaaggg gaaagtattc
     1501 atccacgagt tagcgggaac aggtcacaaa gcccaacttg ttgataaaga aggtactgta
     1561 ttagcagaaa aaactatcgc tccaaatgaa aaagatgggg ctccaatttc agatactgta
     1621 gaatttgaat ttacgggtgt agattcaagt aaattaatcg cgaaagatga attaaaaatc
     1681 caaatcgttt ctccaggttt tgataaacca gaagaaggtt caaccgttat taaggaatca
     1741 ccaaaagcgg ttgataaaca aaccgtggta gttggattta aaccagatgc taaagaatca
     1801 attcggaata ataaaaactt acctgaagat gcagagtatt catggaaaac agagcctgat
     1861 acttctaacg ttactgatag tacgaaaggt attgtaactg ttaagatcgg aaatcgaact
     1921 ttcgacgtgg atgtagagtt tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca
     1981 tacgtaccta taacaacaac tccagaaacg acagttcaaa gtggaaaacc tacatttgat
     2041 aaaccagatg ttcctttagc taaagatgcc ttttcaattt cagatgtcta taataagtac
     2101 ttcggtaatg caagtgttga tgcaaacact ggtattgtta cattcactcc agctaaaggt
     2161 gtaggagaat cggagccgat tactggaaca attcctatta aaattgttta ccaagatggt
     2221 tctgtaggca cgaccgattt agcagtaact gtaagtaaag atatttatga aaatccagga
     2281 gaaaacattc ctgcaggcta ccacaaagta accttcaccg cagg
//
LOCUS       DAFGFZ010000212         2243 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000212 DAFGFZ010000000
VERSION     DAFGFZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.217"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..338)
                     /locus_tag="LR726_002960"
     CDS             complement(<1..338)
                     /locus_tag="LR726_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217969.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNP"
     gene            complement(335..1768)
                     /locus_tag="LR726_002961"
     CDS             complement(335..1768)
                     /locus_tag="LR726_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL6217970.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            complement(1761..>2243)
                     /locus_tag="LR726_002962"
     CDS             complement(1761..>2243)
                     /locus_tag="LR726_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL6217971.1"
                     /translation="AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMY
                     EQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK
                     LANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE
                     "
BASE COUNT          846 a          361 c          269 g          767 t
ORIGIN      
        1 ggattttttt tgtaaacatt ggagaaaagt atttttgtga aataattttc cccctgtata
       61 aaaatttcat cataaacgga taaatcatcc gagaaaaatt gattatggtt agttaacaag
      121 ttaattttac gtttaaagga agaaccgatt ttccggtgat tcacaaaaac aacatcatta
      181 aatagcccta attcttttat acgctcacta tatcttctag cgttttcaaa ttcagaataa
      241 ataaataaat ctccttttga ttctcggtaa aaattttgat aaacatttaa tatattaatc
      301 aaatgtaaag gagtccaagc aatgaaagct gtcttcatat taattctcct ttttttaaat
      361 acaagtaaga aatgcttccc aaataatacc agaaaacaat tgaaatgaca tcgcgcttat
      421 tgaaaagtac atgagcctcc accatttcat ctataaaaat agatagtata aaaattaaaa
      481 caataccaag aaacgcataa ttcactttac ttctaaaaaa tgttttgagt aaactaaaga
      541 tatttaacac atagaaagca tagatacata taaaagcaat tataccactt gatacaagta
      601 cttgaagaaa tccattatgc atgtttccat gtgctctttt tagttcattc acatctgtag
      661 tatcaagatc tacttttttc aaaaattttg catcgctatt cccatagaaa tcggtctctc
      721 cataaccaaa taacaaagtt ggtcttataa ctttaagcgc agctttccaa atcgtaaaac
      781 gaccagctga aatttcggag ttatcactag aatgctgtaa tattacttct ttttctggaa
      841 gtggagtttt atctgattca tttgactcta taacttctac ttttctatca atcgatacag
      901 gaattgttgc taaaaaatcc ttagcataat tagtaccaaa agaaaaatat accaatagaa
      961 ctaataatat gcttaccatc catttgattt ttacaggtag tacttctcta atgctctttc
     1021 taaattttga attggcaaac aacaaaagaa ataatactaa aaaagaacaa attactacct
     1081 gggcacctct agacgaagaa agtgtaatat aaaatatttg tacgataaaa ttcatcatat
     1141 aaaaaacacg aatcgttttt gttatttttg aaattaatac actaattaaa cttaaaataa
     1201 tagaagtata tccaaacata gctccaacat ttggacttgt atagacacca aataaccgat
     1261 gttcaataaa accttgtctt gcaagtaatc ctggcgttcc tgttgggaca tgataagaaa
     1321 tcaaaaaaat aaattgatag atagatatta gcgcagctaa aaaaattatg ataatgaaaa
     1381 cattattaaa atacaccatt tttttctttt tttcctcttc actttgtgaa aagtctgcag
     1441 gaaaaattac tatcaaagta atcagcaaat atccaaaatt ataaatagat ggaattaaaa
     1501 aattttctct gttaatgatt atagttaaga taaaactaat cattgctcca atcagtagcc
     1561 aagaaaaacg cattttaaaa aaatttttct ttataaaaac ctcatacaaa actataattc
     1621 caccccaagc aaaagctatt ttcatcggga tattagttac attaaaagta ctaggcataa
     1681 aataaagcag acaaatgatt aaatttatta ctcgaaacgc atctcgactt aataaactct
     1741 tctctaaaac aggtactatt ctattcatct atatttccaa ctttcaaaat ttctttatca
     1801 attctattta atagtttatc agagtaatcc gatttaactt ttcttacaaa taatttttca
     1861 ctttctttta tttgatctat atccctttca tcaaaccatt tgttcatgga aggatcaata
     1921 atatgaatat ttgctaattt cgaagtttgt aaagtagttt cttcaatatt tttcaattca
     1981 agtggtaaat ctggtcctcc aataacattt ttatttttga acgaggaatt aaaaaaaagt
     2041 gtatgaaaaa atttttcatc tggggcaaac atacctattt taaaattcag gtagtcttgt
     2101 tgattttgtt catacatttg tattaattct gttaggcatt ctttcgacaa agcccaccat
     2161 tgagaaccat gatacaaatc aaatttatgg tgtctaattt ttgggggaag tttaatcatc
     2221 ctcagacaac gattaattat agc
//
LOCUS       DAFGFZ010000213         2224 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000213 DAFGFZ010000000
VERSION     DAFGFZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.218"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            51..758
                     /locus_tag="LR726_002963"
     CDS             51..758
                     /locus_tag="LR726_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217972.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            1139..1318
                     /locus_tag="LR726_002964"
     CDS             1139..1318
                     /locus_tag="LR726_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217973.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1449..1718
                     /locus_tag="LR726_002965"
     CDS             1449..1718
                     /locus_tag="LR726_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL6217974.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1711..1968
                     /locus_tag="LR726_002966"
     CDS             1711..1968
                     /locus_tag="LR726_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL6217975.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          819 a          305 c          459 g          641 t
ORIGIN      
        1 tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata atgaataagg
       61 tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaaacaaa gaacacttga
      121 atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta
      181 ttcaaagagt ggaagaacag ttgtatatgg aaagtgaaac gagtcaaacg aaggttacag
      241 tcagccataa tcgtttagac gtgtataaac gtcgtcctga cacattaaaa aaaggagcgg
      301 agcttctacg actagaaaca gaaaaatata tcatgaatgt ttacctagaa aggagtgagc
      361 tggtcgaaaa ctttgtcagc tacacgattt gcaggaaaca ctattactca gaatcagaat
      421 attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag ctaaacagca
      481 aagatgcgac ggtagacgat tattaccttg tatttactag atttttgacg tgtgggacgg
      541 aatctatcgt gttaatggga atggattatg aactcactgt acccgaagca tttttgaggg
      601 aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta actgattttt
      661 tagacaccta cacttacgac gatacagaag aaatccaaaa tgaacttgac cgtaaacgaa
      721 tgccatatca acgatttgaa aggaaaaaag gtcaataacc atttataccg aaaagtgagg
      781 atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa
      841 tcgatccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgggcaaag cccgataacc
      901 taagagaaag aaggagatcc atatgaataa aatcattcct ctattgttcg tactgtttgt
      961 tggcgagata cttatgcgtc atggtgcagc tctacattta aacttcgtag tattcttagg
     1021 aatactggta ttttgtttga tcttttggcg ctttgtgtta acccttgtga gtctaatatt
     1081 caagttgatt gcagccatat ttttaatagg gttatttcga tgaaaggaga gagtactaat
     1141 gccaattgca ataggaaata aacggttacc cgtgacgttg gatgaaaaga gacaaaaaga
     1201 attgcagcaa ctaaagcaga agtacggcaa aagtgaatcc aggattatgt gtattgcgtt
     1261 agatttattg attgcccaag aaaaagcagg atttgaggta ccagcactca aaaagtgacg
     1321 tcacttttta tcctaaaaac taaaagtgat agcactttta attataagaa gttagaatat
     1381 taatcatttg cttaattgta caatataatg tacaattgtt ttatagaaat aaataagggg
     1441 tgaaaggaat ggaagcagta gcttattcaa atttccgcca aaatttacgt agttatatga
     1501 aacaagttaa tgaggatgct gaaacactta ttgtaacaag taaagatgta gaagatacag
     1561 ttgttgtatt atcaaaaaga gattatgatt ctatgcaaga aacgttgaga acactttcta
     1621 ataattacgt catggaaaaa attcgtcgag gagatgaaca attctccaaa ggtgcattta
     1681 aaacacatga cttaatcgag gttgaatctg atgattaagg cttggtctga tgatgcttgg
     1741 gatgattatc tttattggca tgagcaagga aacaaaagca atataaaaaa gattaacaag
     1801 ttaataaaag atatcgatcg ttcccccttt gctggattag gaaaacctga gccattaaag
     1861 catgatttat ctggaaaatg gtccagaaga attacagatg aacatagact gatatataga
     1921 gttgaaaatg aaacgatatt tatttattct gcaaaagatc actattaacc aatcggaagt
     1981 aaggaaaggg tcagaaactt aaaagttttt gatccttatt ttatttaccc tagtcattta
     2041 aaaagctaat atagcttagt gttgattgtt attaatgaat gtgtttgtta cgcgtattac
     2101 ggatataagg ttagtaaaat catttctaaa gttgaggaaa agtaaatata aatggcttaa
     2161 atttcaacaa tttgaagttg aatagatatg ttataatact attgtagtgt gggatgttag
     2221 ttac
//
LOCUS       DAFGFZ010000214         2218 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000214 DAFGFZ010000000
VERSION     DAFGFZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.219"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..949
                     /locus_tag="LR726_002967"
     CDS             <1..949
                     /locus_tag="LR726_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL6217976.1"
                     /translation="RISRVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQR
                     KLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLK
                     ETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVS
                     NKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSS
                     LYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKE
                     EQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKMFV"
     gene            963..>2218
                     /locus_tag="LR726_002968"
     CDS             963..>2218
                     /locus_tag="LR726_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL6217977.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          796 a          374 c          457 g          591 t
ORIGIN      
        1 gagaatttca agagtaccaa agcaaataaa accgggtgta ttcaatcagg ctttgattag
       61 acttagaata aataaagaaa tcacagattc agaatatttt attcaattta ttcgtgcaga
      121 ttttatgcaa agaaaactca caggagctaa tccaggttca gcaattacaa atctcgtacc
      181 aatgtcagaa gtgaaaaaat ggatagtaca atttccaata ttagaagaac aaaagaaaat
      241 tgggaacttc ttcaaacaac tggacgacac tatcgctctt caacaacgta agctagattt
      301 attaaaggaa acgaaaaaag gattcttaca aaaaatgttc cctaaaaacg gagcaaaagt
      361 gccagaaatt cggtttcctg gatttacgga agattgggaa cagcgtaagt tcaaagagtt
      421 tagtaagaag actggaaaaa agaacacgaa agatttagac ttccctgcct attcggtaag
      481 taataaagca ggtttaattt ctcaaacaga acagtttgat ggtagtaggt tggatgattt
      541 agaaaaaaca aattataaat tcgttgaacc aaatgaattc gcctataatc cagcaagagt
      601 aaacgtagga tcaattgcat ttaacaatct tggtatgacg gtcattgtta gctctcttta
      661 tgtagttgtg aaaatgagcg aggacttaga taatgagttc atacttcagt ttatcaagtc
      721 accaacattt atcaaagagg tcaagcgcaa cactgaagga agcgtacgag aatacttatt
      781 ctatgagaat ttcgctaata tcaagtttcc ttttactagg aataaagagg aacaacaaaa
      841 aatcggcgct ttcttcaaac aactcgacga cacgatcgct cttcatcaac gtaagttaga
      901 tttgctgaag gaaacgaaaa aaggcttctt acaaaagatg tttgtttagg gagggaagat
      961 aaatgaaaat tagtcaccgt gaggaagcgg aagtcgaaga acagttgatc cgtgtattgg
     1021 gggaagggca taatcagtgg acgtatcgtc ccgatctaaa atcagaagaa gatctttggg
     1081 tcaatttacg ccaaaaaatc atctcaaata atcaagcaga actgaatgat tttcctttga
     1141 ccgataaaga atttgaaacg atcaaaacag aattgttact acgaacgaaa accccctttg
     1201 atgctgcgaa atggttaaaa ggtgaaaatg gaatggctcg gatcacgatt gaacgggaag
     1261 atccgcaact gggttctgtc tcgctcattc tttattctaa tcaagacatt ggtggaggaa
     1321 tctctaccta tgaagtggtc catcaaattg caaaacgtgg aagtaacata gaagcacggg
     1381 atcgacgttt tgacgtcacc ttactgatca atggattacc gattgtccaa atcgagctga
     1441 aacaagtaac cgcaaaagat agtttttatc aggcgtttaa ccaaatcaaa aaatatgcag
     1501 aagaaggtat gtttcggaac aatatctttt cgaccttaca gttgtttgtg gtttccaatg
     1561 aacaaaccac tcggtatttt gcaaatgcct taccaaagga tttgcatcct aagtttttat
     1621 ttagttggcg aacgaaagat aataaaaagg tagaaaactt atatgagttt tgcaaacaag
     1681 tgctaaatat cccggatgct catcgcttga ttgcggatta tacgattgtc agtgaagatc
     1741 aagacaataa aatcctcatg gtgttacatc cttatcaagt acatgcgatt caagccttgt
     1801 ttattgctgc aaataaacat caatcaggat atgtgtggca tgcgactggt tcaggaaaaa
     1861 cgttgactag ttttgtatct actaagcttt tagcacgaaa atcggggatc gatcggacga
     1921 tcatgttagt ggaccgcaaa gatttagata atcaaaccac cacagagttt acgaaatttg
     1981 cttcggaatt taatactggg atttcttccg ggaacgccaa agcgaatagc ttgatcgtgg
     2041 gaacaggaag tgccaaagag ttaagtgaaa ctctgttggc agatgccaat gcaaatgttg
     2101 tgattattac aacccgtcaa aagctagatg cggcgttgaa atatgcaaaa aaacaagaag
     2161 agaaaaaagg aaccaatcgc ttccaaaaat taatggggca acatatcgtg tttgtagt
//
LOCUS       DAFGFZ010000215         2170 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000215 DAFGFZ010000000
VERSION     DAFGFZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.221"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            278..787
                     /locus_tag="LR726_002969"
     CDS             278..787
                     /locus_tag="LR726_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL6217978.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LR726_002970"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LR726_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL6217979.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          756 a          379 c          418 g          617 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca
//
LOCUS       DAFGFZ010000216         2154 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000216 DAFGFZ010000000
VERSION     DAFGFZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.222"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            79..1125
                     /locus_tag="LR726_002971"
     CDS             79..1125
                     /locus_tag="LR726_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL6217980.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="LR726_002972"
     CDS             1474..2106
                     /locus_tag="LR726_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL6217981.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          801 a          295 c          436 g          622 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctaacaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa agaggtacta tttaggatcg tatg
//
LOCUS       DAFGFZ010000217         2142 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000217 DAFGFZ010000000
VERSION     DAFGFZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.223"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            57..497
                     /locus_tag="LR726_002973"
     CDS             57..497
                     /locus_tag="LR726_002973"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL6217982.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            625..1308
                     /locus_tag="LR726_002974"
     CDS             625..1308
                     /locus_tag="LR726_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL6217983.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1617..1690
                     /locus_tag="LR726_002975"
     tRNA            1617..1690
                     /locus_tag="LR726_002975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1651..1653,aa:Arg,seq:tct)
     gene            1849..>2142
                     /locus_tag="LR726_002976"
     CDS             1849..>2142
                     /locus_tag="LR726_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL6217984.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTS"
BASE COUNT          711 a          335 c          435 g          661 t
ORIGIN      
        1 cttttataag ttctataacg ttgattatat ttttaattct tattcttctc gtcgtcttgg
       61 ttccccgaat ttttggtttg actccttata ttattgagga caaagaaatg agtccatatt
      121 atgcacaagg aaatctgata tatgtacgac cgaaaaatac aaatgagatt ttagtcggag
      181 atattattac atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg
      241 ttgaaaacaa ccacgaagat ttttatacaa aagctgatac aaagacttat attgagcctg
      301 gagtagtaaa aaagagaaat attatcggta gtcccatctt tcagattcca tatattggac
      361 tagtcctttc aaaaacggca tttgcatgga ttcaggggtt attctttacg attgcttttt
      421 gcctaacagg aatcacagca tggaatacct ttgtagagtt gaaaaagaag cggagacgaa
      481 cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat tccagagatc caacgactct
      541 tctgaacatg aaacacgaga aaacactcat gcagcttgta gatttagtca gttgttcgta
      601 taatgtgtga agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa
      661 gaagaactat tggattttat gcgtacccag caaaaaaaat taccgggtga gctaggcaaa
      721 ctggaagaag aagctcatgt agcagaagta ccagttattc cccatgaaac agttgtattt
      781 ttgaaatttt tacttggaca gcttcagcct gaacgaatct tggagatcgg agcagctatc
      841 ggtttttcat caagtgtcat ggccacgaca atcggagaaa acgctcatgt tacgacaatt
      901 gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact atgaacgtct aggactgaca
      961 gataaagtga ctttgcttga aggtcaagca gcagatatct tgccggagtt gtctggtccg
     1021 tatgacttta tttttatgga tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc
     1081 ttacgtttgt taagaaaagg tggcgttttg atggtagatg acatcttcca aggaggaacg
     1141 atcttgctcc cagatgaaga gattccccgt ggcaaacgag ctatccatcg taaattaaat
     1201 gaatttcttc gtgttgtgat ggatcatccc gatttgacat cgacaatctt gccattaggt
     1261 gatggtgtga tcttaatgac aaaggaatca gaaacaatcg agttgtgata catgattatg
     1321 gcagcctgta gataatcgat tgaatcgagg gctcaattcg aacttgatgt aaaagtttct
     1381 atatttgtaa gtcaattaaa ggatacagga aatctaaata aacaggagta gcatgaagtc
     1441 aatgtagctc ctgttttcaa ttgataacct tagaacagct gaaggagaat gtctcgtttt
     1501 aaaagattga tttagtagtt gggccaaaat aattcattat ttgctaatta aataactatt
     1561 tcgtattgac agaagcaatt taattatcta taattattga tgttccgctg attcttgtca
     1621 tagtagctca gctggataga gcatccgcct tctaagcgga cggtcggggg ttcgaatccc
     1681 tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta acggtttttc tttgtttttt
     1741 gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt atcgagtaca
     1801 attataagga gcaaaaaaat aaaactcatc agaaaagagg aagaagatat gacgatacta
     1861 tctagaattt tagtgactct agttgcaatt gaattttttt atatcatgta tatcgaaacg
     1921 gtgagaactg attctgacac gacaagtagg gtttttaaaa tgagtaaaga agagttaagc
     1981 agaaaatctg ttcaaacatt gtttaagaac caaggagtgt acaatggact attaggagtg
     2041 ggactacttt atggagctta tctttcttcc gcatctaaag aaatcacaag tatgctatta
     2101 atatctattt tttttgttgc attatatggc agtttgacta gc
//
LOCUS       DAFGFZ010000218         2131 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000218 DAFGFZ010000000
VERSION     DAFGFZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.224"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(94..1020)
                     /gene="cysK"
                     /locus_tag="LR726_002977"
     CDS             complement(94..1020)
                     /gene="cysK"
                     /locus_tag="LR726_002977"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL6217985.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1160..2047)
                     /locus_tag="LR726_002978"
     CDS             complement(1160..2047)
                     /locus_tag="LR726_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL6217986.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          590 a          475 c          356 g          710 t
ORIGIN      
        1 attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt aaaagttttt
       61 tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa gggctgtaga
      121 taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt ttcctaattc
      181 ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta ctaaaatccc
      241 ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa tagaaaggac
      301 tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga ttccttggat
      361 tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt ctaccccgat
      421 taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga tcgttcctcc
      481 tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt gatgtatctc
      541 aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt gtaatggtaa
      601 aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag aaataccatc
      661 cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac gtctttcgat
      721 actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc ctaccatcga
      781 caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg gtttcagtag
      841 accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta cacttcctcc
      901 tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta cgattttatt
      961 taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga tagctgtcat
     1021 cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg ttcatttatg
     1081 gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat atggtaattt
     1141 ctttgccttt ttatctaaat caaacgttta gttcttgacc aactaataaa gaataactgg
     1201 acatagacat gccatttttc gccattagat catcaacaga catgccaaat ttttgtgcaa
     1261 tatcccatag cgtatcacct gacacgactg tatatgtctg accattattt gtatttacat
     1321 ctgttgttga agatgatgac gttgaagtcg tcgtagtagt cgttgttgtt gtacttgtgg
     1381 ttgttgtagc cgaacctgtt gatggcgtat caaaacgggt taaattataa gtattaatca
     1441 atgagttcaa tttggcccca taatttggat cggtagcata acgacctgtc aagtacgctg
     1501 ttgcatcata aaaacttgtt gtattgcttt tccacacgcc agcataatga taatcgcccg
     1561 tggaaaaact ggttgaacga agaacattcg catggtcttg gaaggattcc caataagaag
     1621 aatattttcg gaatggctca ttcatcgtta cccattgtcc gtttacatat tctttggttg
     1681 ccatataaac gacctgtccg ttatagcttc ctttcacacc gaatagatta taatttggcg
     1741 cactagctaa tccggaagtg ccatatgcac tttccaacaa cgcttgagcg atcatgactg
     1801 aagcatacaa gtcattactt gcagcaactt ctgacgcaga ccagccgatt tgatcgataa
     1861 acgcttgagg atcagcgact gtttgagatc cttgttcatc tgcatgaaca tgttgcggta
     1921 ccgctgtaac catcatcggc ccaactaaaa gactcgttcc aattaaagga gcaatcgctt
     1981 ttttattgat caccattttt cttttaggtc tatggtgccg ggcactgcgg gtcttcagct
     2041 cttgcattca aattcctcct caaaataaaa aaacttaata aaaagttctc ttcttaaaac
     2101 aaacttaatt aattaaatta tacttttaat t
//
LOCUS       DAFGFZ010000219         2104 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000219 DAFGFZ010000000
VERSION     DAFGFZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.225"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..523
                     /locus_tag="LR726_002979"
     CDS             <1..523
                     /locus_tag="LR726_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217987.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2053
                     /locus_tag="LR726_002980"
     CDS             584..2053
                     /locus_tag="LR726_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL6217988.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
BASE COUNT          804 a          327 c          457 g          516 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc
      241 tgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt
      361 aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaattca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgct gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg
     1081 tttagatcat gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc
     1141 aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca
     1201 cattgacaag aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca
     1261 tttaaaattc tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa
     1321 actacaagat gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta
     1381 cgttgaagag tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat
     1441 atgtgtggac aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat
     1501 cgttgatcct tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca
     1561 agtacctaaa gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc
     1621 tgaagcaatt atggccatac acaaaactga aactaattta cagctgacaa atcaattggt
     1681 gtctgctcat gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga
     1741 aacaattgat ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg
     1801 cgcagatgga acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt
     1861 taatgcagga atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca
     1921 tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat
     1981 ccagcaacaa gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca
     2041 agaaagggag tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag
     2101 cgaa
//
LOCUS       DAFGFZ010000220         2073 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000220 DAFGFZ010000000
VERSION     DAFGFZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.226"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(510..875)
                     /locus_tag="LR726_002981"
     CDS             complement(510..875)
                     /locus_tag="LR726_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217989.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(951..1196)
                     /locus_tag="LR726_002982"
     CDS             complement(951..1196)
                     /locus_tag="LR726_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6217990.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1266..1520)
                     /locus_tag="LR726_002983"
     CDS             complement(1266..1520)
                     /locus_tag="LR726_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217991.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1684..2007)
                     /locus_tag="LR726_002984"
     CDS             complement(1684..2007)
                     /locus_tag="LR726_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217992.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          779 a          356 c          366 g          572 t
ORIGIN      
        1 agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg ctagcaatgg
       61 caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact gaagccacga
      121 cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat cgacgatgca
      181 tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga aggtgtagaa
      241 gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt gcatcgtata
      301 cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt atgtctaggg
      361 aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag gatgaagata
      421 accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt taatgaagta
      481 agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt ttgctccaaa
      541 tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg cacgttgcgc
      601 tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat aggatttttc
      661 cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag ctgatgatat
      721 tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg tttctttccc
      781 cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg ttgacgagga
      841 agctttgaaa tcaatttttc atgccataat cgcatagaaa tgtctccttt atatatagat
      901 tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt
      961 ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta aaagtcagcg
     1021 atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt acgatatttg
     1081 aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg gccagttctt
     1141 aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat actcatttaa
     1201 tcacctttca ttatagtaga atagttataa tgactataga aagctacaca tgcaatcaat
     1261 agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa taaagacaaa
     1321 accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa
     1381 actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa ccgttctttg
     1441 ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa
     1501 actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata aaagataaca
     1561 tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata gatcaatgaa
     1621 aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct gaatgtgtct
     1681 tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat ataaaaagag
     1741 gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg aaacatattt
     1801 aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga gcatcatgac
     1861 aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa ttcctgaggt
     1921 ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata aaatcgcaat
     1981 aaatggcaaa ataatcactc ctaacattga gaataaataa aagaataaca ttttttctaa
     2041 aataagcaaa taaaaattaa agtaacaaaa tag
//
LOCUS       DAFGFZ010000221         2072 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000221 DAFGFZ010000000
VERSION     DAFGFZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.227"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(642..932)
                     /locus_tag="LR726_002985"
     CDS             complement(642..932)
                     /locus_tag="LR726_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217993.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1140..1831)
                     /locus_tag="LR726_002986"
                     /pseudo
     CDS             complement(1140..1831)
                     /locus_tag="LR726_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          586 a          387 c          306 g          793 t
ORIGIN      
        1 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
       61 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
      121 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
      181 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
      241 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
      301 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
      361 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
      421 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
      481 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
      541 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
      601 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
      661 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
      721 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
      781 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
      841 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
      901 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
      961 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
     1021 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
     1081 ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt taagaaagat
     1141 tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc ttccacttca
     1201 tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc agcagttgct
     1261 tcgtctaatt tcttttgtgt atctgttaac tgatttctac atttgttttt tcttgtgtca
     1321 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     1381 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     1441 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     1501 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     1561 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     1621 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     1681 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     1741 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     1801 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     1861 catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat
     1921 tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc
     1981 ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat ggtgaacaaa
     2041 aacaaaaaag caagttttat taaacgtaac cc
//
LOCUS       DAFGFZ010000222         2065 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000222 DAFGFZ010000000
VERSION     DAFGFZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.228"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            322..1362
                     /locus_tag="LR726_002987"
     CDS             322..1362
                     /locus_tag="LR726_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL6217994.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          782 a          275 c          335 g          673 t
ORIGIN      
        1 aatggtagta tgttttatat gcataaggta gttcttaatt tattttttgt tttgacactg
       61 gtagtactta attatcttta attttaaaac taaattttat tcatacgttt ttctggtagt
      121 atttaattat aaataatatt tatttaaagt agttcttaat gactttaaag gtagtacata
      181 attcatttta tttctgattt taggtagtat ttaattaaaa aacagtttta atgcgtttgc
      241 attttttatt tatctttgct attttcgaaa agagtaggta attttacata aataaaacaa
      301 cgtgaaagta gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac
      361 aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg
      421 aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc
      481 aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt
      541 agagtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt
      601 ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat
      661 agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt
      721 gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc
      781 gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc
      841 caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta
      901 gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta
      961 gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta
     1021 ttaaatttga taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact
     1081 attcataatg caaagaaaaa agctgaaata gaaagtgata tttcgatagt ttttgaagaa
     1141 ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat
     1201 caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa
     1261 aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat
     1321 gcaagctccc caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg
     1381 attgttgctt catggagtaa cattaaaatt aaaaattttt tattattcta attatattac
     1441 gaatattaca aaataatagc tattttaata gaatttataa gttaaaatat tatgataaaa
     1501 atcacaaacg tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag
     1561 aaaagggaac gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag
     1621 ataatttcat tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga
     1681 atattattcg cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg
     1741 attttcgttt tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa
     1801 ggcggtatga actcggaagg ctggtaacct tcgtcagcgt tcatacacgg cattaagatt
     1861 tggcccttaa tgcgttaacc tttcagtgtt acggtattac ccgcaatgga tttagtataa
     1921 cagatattca aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat
     1981 tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta
     2041 tgacgaacgc tgaaattaga gggaa
//
LOCUS       DAFGFZ010000223         2001 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000223 DAFGFZ010000000
VERSION     DAFGFZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.230"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            322..1122
                     /locus_tag="LR726_002988"
     CDS             322..1122
                     /locus_tag="LR726_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217995.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            1159..1341
                     /locus_tag="LR726_002989"
     CDS             1159..1341
                     /locus_tag="LR726_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217996.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            1531..1818
                     /locus_tag="LR726_002990"
     CDS             1531..1818
                     /locus_tag="LR726_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217997.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
BASE COUNT          815 a          213 c          401 g          572 t
ORIGIN      
        1 aaaagagagg gagtgattag aactttgagc aattcactaa ttgtttaata atagaacgat
       61 tcctaagaag ttcattgttc attggcaaat tgctttatca gattgaaagt tggcgaataa
      121 ggtttgtaga ataacacctt ttcaagcaaa gcgatttttg aaagctatga aatttgttta
      181 ataaaaaaat tcaccagata atctgataga gactaaccca attactaaca gttgtaattg
      241 gtgttagctt acaaattaat tattgtgttt agtcaattac aaattgtgaa acggcactaa
      301 taattggaaa ggaaaaatga aatgtctaaa gatttacgag agcttaaatt gcgaaaaaag
      361 caatataatc aatatttttc tagtttgttg aataaacata ttgaagaagg acttgatcat
      421 gattttggaa aagatgaata ttgtttatat tgcgaacgtt acaatgaaaa aatgtctgaa
      481 tatctacagc tgaaaaaaga gatacagaaa ttagagaaaa aagtacatgc ttggaagtat
      541 aaagattcca ggtctggtta tgttgaatct caaaataagc caagaaaaaa gaagcaagcg
      601 tatgttctaa agatggtaaa tggggaagag ttaattgtat tagcgtacag tgaagaagag
      661 gttcgagaac tcgttgaaac acctgtcagg aaaaaggttg aaacaataaa tgagatagac
      721 gacagccaat acaatagcat ctatattgaa acaagtcatg gtggatataa gaaattagca
      781 actttcctta gaagttctaa agttgggata atttctaaaa aagacataag tgctgttacc
      841 gatagttttt gtactttatc tgttcctaaa gcttatttcc aatacgatag taaaagattt
      901 gaacgcttag agaaattctg tagaccgtat actgtattga aaactaaaaa atttaataaa
      961 actaaaagag tttcgataca gttaccgact ggtcaaatct cattaggtga aaaagactat
     1021 ctgataaaag taggaaaaga gttaattata aaagtaccgg aacacttatt taaagaaaat
     1081 tttgagatac gtgaaacctt ggtatggaaa gaagaaaaat agtttggaag acatagaatg
     1141 gtataagggg aaattgaaat ggcagtaaat aaaatattgc aggtattaat catagacgaa
     1201 tttggcggag gagaatggtt tgatgtgcag tccatagact ttgaagaaga agtcgtagtt
     1261 atagaaaata tttacggcga agtatactca tattcgcttg aaggtgagaa tgttgaggat
     1321 ctacggttga ttggtgagta gaaaaaagca gaaatatcaa aatggagata aaaattaata
     1381 gcatgtaaag atagtcatgc ttacatgctt tatccaaaat ggtatgcaga caaaaatata
     1441 gctgaaataa acgattatct aactagtata aaggaacgca atgcaattaa ttaacaatag
     1501 acgaaaaatg aaattaaagg agataggaaa atggtagaag ttgaatttgg atcaaaagaa
     1561 tggtttgaag agttaaactc aatgattaag ttcccagaaa agtttacttt ttactacaat
     1621 aaaagagaca atatcattca agtagcttta cttgaaacta agggaagatt agaaaatact
     1681 cgatggagta tgataaaacc atcggcactt agatttcatc ctaaagactt gacagcggtt
     1741 gtagcggtaa aagaggttca agactcacaa gaatattatg aagtagtaaa aaatctgaag
     1801 gatatttggt gtaaataatt ctaaattgac cagttatttg gtcaaaaata gtatgtgatt
     1861 atttcatcca atgtggcatc ataattggta tggaagggga taaattattt gaaagaaata
     1921 gaaggtatta agaaaagaaa tcaggaaaga ttggaactgt taaatgaaaa tagaaataga
     1981 aatagaatgt ttagttatta t
//
LOCUS       DAFGFZ010000224         1993 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000224 DAFGFZ010000000
VERSION     DAFGFZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.231"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(80..430)
                     /locus_tag="LR726_002991"
     CDS             complement(80..430)
                     /locus_tag="LR726_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6217998.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(423..1748)
                     /locus_tag="LR726_002992"
     CDS             complement(423..1748)
                     /locus_tag="LR726_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL6217999.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          563 a          410 c          303 g          717 t
ORIGIN      
        1 tattattggg aatcctagct taaatcatag ataccgtaag ggattttatt ctttatttaa
       61 aactttgcaa cagaaccgtt cattctaatc gactccattt attatcctgg taaaattcga
      121 tatgacgtat ttcgtcgtag tgaattttgt cttttccaac gtagatcccc agctcatcac
      181 tacctaagat cttgccaaca atatcttctg gatagtatcc ttcagcgttt cgttcttctt
      241 tttgaataga tactgatcta ttttttatga ctgcagcgtt caatacttcc gcaatctctt
      301 cagtcgtcat ttgttctttt gctaaatttt gactggttcg ttgttcattg tcggcatcaa
      361 tttcttcagt atgatcagat aaataaaacc ctgcccactt taaacgtcca cgatcattgt
      421 aatcaatcat ttttaattcc cccatttccg cccgcatgac caccaacaag tgtgcttcta
      481 gtaatgcttc tagcgctttc taacattgaa gaagcatgta caatcgacgt aaaaccatat
      541 ttttgccgaa tcttgtcaat aattttatct aaattttctt cgttaatttg cttttgtgga
      601 tcactaaata aatccagctg aagcgaatcg gtgtacacaa gcttgctata ggttattcca
      661 acgtgcctta cttcttgtcc ttcataatac ttatcaaaca agaaaagaca ataattctgc
      721 aattcctttg tattatctgt gataggtatt ttcatctggt gtgaaaatcc tggtctcgtt
      781 tctaagatcg acgtaccaat gtttaaatga acacatcctg ttttacaatt atgctgcctg
      841 atacgaatgg ctacttgttc cgccatttct tttaccacca gctcgatttc atttcttcta
      901 gtataatctc gtggtaacac ttgtgaatta ccataagatt tttcctttgt cggttctggc
      961 gggattgcta tatctgttcg atcgattccg ttagcatgga agtacagctg taatccaatc
     1021 acacctaatc tattcttaat cgtataagga ttccagttag caaggtctct aatgctcaaa
     1081 attcccattt ggttgagcct ttttttcatt cgagaaccaa ttccccaaaa gtccgtcatt
     1141 tcgggaatat tccatacttt ctcaggaacg ttttcgtacg tccattccgc aataaatcct
     1201 tcattatgct ttgcttcatt gtctaaggct aatttagcca aaaggggatt atcgccgacg
     1261 cctaccgtag caatcaaacc taactcttct ttaatacgtt cttggatcat ttgagcgagc
     1321 ttcttccttc gctgacttcg tgtttcttca gtcgtaaaaa gattcagtga ttttgtcact
     1381 tttaaaatcg attcatcgat cgagtaaatc agtagatctt catcagccac atatcttctg
     1441 aaaatattat ttacctgcat atttcgcttg atatataggt tcatacgtgg tggaacaacg
     1501 tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc tactcacatt tgtaatacca
     1561 taccgctttt ttgcttcagg agaagaagct aatatcaatc ctgaaccagt attatcagat
     1621 cgactcataa caacaagttc tgttgttaat ggatctaaat ttctttctat acattcgaca
     1681 cttgcgtaaa agcttttcat atcgattaaa aagtaatcat ttactggctc ttttgaataa
     1741 tccatcataa ataacactcc attcttccac attatacaaa catatgttcg atgttgtaaa
     1801 gaagaaatag ctaaatacat aaaaaaagaa gcataggtat gctatcaccc tgcttcttct
     1861 tatgatttgt atgatcaatt tattctatca ctttactaat taatttgaaa atctaataaa
     1921 aactctcact ctaaaagagt gagagactgc aaattagagt acggactttt ttggagttcc
     1981 gttctttaat att
//
LOCUS       DAFGFZ010000225         1985 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000225 DAFGFZ010000000
VERSION     DAFGFZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.232"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            86..1279
                     /locus_tag="LR726_002993"
     CDS             86..1279
                     /locus_tag="LR726_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL6218000.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1445..1867)
                     /locus_tag="LR726_002994"
     CDS             complement(1445..1867)
                     /locus_tag="LR726_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218001.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          525 a          337 c          407 g          716 t
ORIGIN      
        1 ttcaaataaa aaagtcttgt ttttttattc ttttttggat atacttttaa caatttaggg
       61 aaattttagg aaaaggggta taaaaatgac aacgtatcag cgaaaggtat tattatcgac
      121 atcggcggga attgctttag agaatatgga tattatgttt ttagcttttt cattatcttc
      181 aatgattact tcttttcata ttagtggtac gcaagcgggt ttcatttcaa cgatcactaa
      241 tttggggatg ctcattggag gtattttttt tggcatcata gctgataaat atggacgtgt
      301 gaaagtattt tctcaaacgg tccttctgtt ttcgattgct tctttattga tgtattttgc
      361 ttcaaacatc tacttggttt atctctttcg ctttatcgca ggtgtaggag cgggaggaga
      421 atacggggcg tgtatgtcct tgatttccga aagtttttca aaaaaacaaa ttggacgagc
      481 ttcttcggtt gctggtattg gtgcgcaggt cggtgcggct ttggctgcga ttttagctgc
      541 tgtgatcatt ccaacgtttg gttggaagat gttgtatgtc gtaggtgtac tgccggtgtt
      601 gatggtcttg ttcattcgtc gcggcttgac tgaaccaaag gaattcaaat ctgtacaatc
      661 tgccgaaaag aaaaccaaac ttagcagact gtttgaatca aaacatcttg cttggcaaac
      721 atgtggcctt agtatcatgg taactgtcca aattgccggc tattttgggt taatgaattg
      781 gctgccttca attatgcaaa atcgattagg gctttcggtt tctgggtctt cattgtggat
      841 ggtcagtact attttaggca tgtctttggg gatgatgact tttggcataa tcatggataa
      901 attggggcct aaactagcct tttctttgtt tttaatctgt tcatccttat ctgtctatct
      961 acttgtgtta gcccatagcc agtggagttt ggtagcagcc gctgtgatag tcggctactt
     1021 tatcaatggc atgtatggtg gatatggcgc agtgattagt agtttgtatc caacagaaat
     1081 ccgcgcgacg gcgaataatt tcattatgaa ccttggacga gctgttggtg gtttttcttc
     1141 tatcgtcatc ggtttcttga tggatcatta tagtttaact gccgtaatct tgtttttgag
     1201 cggtatttac ctagtcagtc tgctggtctt gacaactctt actggtgtta cagcattaag
     1261 ggaaagcttg cagcattgat agttgagtct gtaaatgaca agaaagaggt gtggcaaaag
     1321 cttgcatctg cggtaataag accagccaaa tcaaaaacat gagacactgt ttccgtttgg
     1381 ctggtcgtat tacttgatgc agggcagctt tttcaactgc taatttcctt ctatctggcg
     1441 gcgactattc tttgacgtcc gtcatttcaa agtactttac taataactta gcataacgaa
     1501 aaactgtgga tacactcgta tattgagcgt ctgcataatc ttccaaattt ttctcaggat
     1561 gttgatacat gaattcaatc aaatgataat tatatgattt tctgtagaaa tgaagataaa
     1621 taatgtagat tgggaagttc tcctgctttt ccatagaaaa gcgccgatta ttctcattct
     1681 tcaaaatgac cttatcagga tatttttgga acaattcttc taaatctgtt agataattca
     1741 agatagactt acttgatacc ttcaattcgt tttgtagttc ttctattgaa caccaacgtt
     1801 ctgtttcaag cagtaaatga atcattttaa ttttcttttg agaaactttt gttaaaaaag
     1861 tctgcatatt ttctcctcct gtatctactt tactatacta catacaagaa aaaaaacgga
     1921 tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat ttctcttttt
     1981 ttcct
//
LOCUS       DAFGFZ010000226         1958 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000226 DAFGFZ010000000
VERSION     DAFGFZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.233"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..487
                     /locus_tag="LR726_002995"
     CDS             <1..487
                     /locus_tag="LR726_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL6218002.1"
                     /translation="VLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKLDQQGYVTVLD
                     KDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLDKTTATGLLFT
                     REQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQFF
                     E"
     gene            complement(686..1132)
                     /locus_tag="LR726_002996"
     CDS             complement(686..1132)
                     /locus_tag="LR726_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL6218003.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(1179..1892)
                     /locus_tag="LR726_002997"
     CDS             complement(1179..1892)
                     /locus_tag="LR726_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL6218004.1"
                     /translation="MQKNAKTLERRLQKIGETTKPQQMKQIRFPVPKSLELHSRYPIM
                     GQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGSGKTTLLEHIRKQGEGILLSPKVS
                     FQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSLLNHLGFAQETLTKPLCTLSGGEA
                     TRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEKLMQIYPGTILFTSHDSYFVERTA
                     DEVYEIKGQKIKKVLTRNF"
BASE COUNT          622 a          407 c          346 g          583 t
ORIGIN      
        1 cgtgctttta tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg
       61 ggaagcccat cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt
      121 gaccgttttg gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg
      181 acgtgctctt gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga
      241 atttctagca aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct
      301 attcactaga gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga
      361 cctctcgcta gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat
      421 gctttgtcag caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt
      481 tgagtaaggg ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa
      541 taaaagagca atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg
      601 ttcggatcac tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg
      661 ccttttttca aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc
      721 ttgaaagcgg gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc
      781 aagcattcat ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga
      841 ataaagacat acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt
      901 ggaccttgct ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga
      961 tgatgaagac atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac
     1021 ttgataaatg aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca
     1081 aaaaacacat cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc
     1141 actcctttaa atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact
     1201 ttttttattt tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa
     1261 gaatcatgag aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct
     1321 tcgatcgttg ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc
     1381 ttagtaaaaa gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac
     1441 ggtttcgtca aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat
     1501 tctgaaaact ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga
     1561 taaccctttt gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct
     1621 tgtttgcgga tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg
     1681 atttttttac ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt
     1741 tccaattgga cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt
     1801 actggaaaac ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt
     1861 cttctctcta atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta
     1921 gaggaagaca accgatccag tctgatttgt tgcttttt
//
LOCUS       DAFGFZ010000227         1954 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000227 DAFGFZ010000000
VERSION     DAFGFZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.234"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..425)
                     /locus_tag="LR726_002998"
     CDS             complement(<1..425)
                     /locus_tag="LR726_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL6218005.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="LR726_002999"
     CDS             complement(534..1565)
                     /locus_tag="LR726_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL6218006.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          602 a          323 c          339 g          690 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tact
//
LOCUS       DAFGFZ010000228         1950 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000228 DAFGFZ010000000
VERSION     DAFGFZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.235"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..483)
                     /locus_tag="LR726_003000"
     CDS             complement(<1..483)
                     /locus_tag="LR726_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBL6218007.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(485..1921)
                     /locus_tag="LR726_003001"
     CDS             complement(485..1921)
                     /locus_tag="LR726_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBL6218008.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
BASE COUNT          595 a          396 c          210 g          749 t
ORIGIN      
        1 tttacctttt ttgtatgctg cactgtcaat ccaagttcct aaattgttgt atccatcatc
       61 acttatataa tcatattggc catcaataat ttcgtcaaac cacaaatatt ctccatctgg
      121 tttttgccca attattttaa ttactgattg actagtagtt gtcatctggt taaaatcttt
      181 tattttacct atattaacgg ccaaaatagc atttcctcgc ttataactct cctggatttc
      241 atatttaaca tattctcgat caagagtttc tttacctatc agcaccacag tcactgatgt
      301 tccaattaat tgattatcta tccagttttt tatagcaata tctccattac gtttaacttt
      361 ctcaaattca gccttatcta caaaaccagc ttcctttgtt cctcgcaaag tccagctgtt
      421 tctaactacc attgctcttg ttacatctgc ataatggaaa ctaaaaaaaa cccttctcac
      481 catattaatc ctcctgaata ctatttatta aactgacaat ttgttcaact tttttatcta
      541 cttctccttc agtatctaat ttttctagcg cttgaaattt actttttaag gaatcatcaa
      601 aataatcact gaaattctct tgaatcttat caaacaaaac cttacttgca cctttggtaa
      661 atccaatcgg aactactata ttttcatttt gaacagataa ttcaaattct tcaatcattc
      721 catctgctgt aactatttca cctgattcaa gtttgtttcc aaaaacaaaa atactaatcc
      781 cagactctgc aataatgttt tctcgatatc ttctccactc atgaggcata tcaaggggaa
      841 gtggtcttaa agaaagtacg tcttgtatgt tattttttct taaatttctt ctctgcttta
      901 gaactcctga aacaatacta ttaccaattc caagtccgta acctgatact attctataac
      961 cggattcaga aagcctatat gctaatttct cgacaaattt taatcctttc gattcagatg
     1021 taaatggagt ataatcaaca gcacttcccg aaataaaaat agtttttctc aaatactttt
     1081 ttctaatact tttcaaaata tcagtaatct cagaataatc atctacttca acaacgttta
     1141 tcgaaaatct ggacaaatca ttgatttgta gtcttctttt tgtcagttca taattatatc
     1201 tctcttgatc aaaaaaatcc tcagaatcga caaaattttc ctttaatatt tttctcatta
     1261 tacaataatg aggtctttgg ttttctctca aaacccatct tatatctgat aaaattcttt
     1321 ctaaattagg gtctgtaaaa ctaaatccta aaaagagaaa agtttttgtt aacaaatctc
     1381 cttttaaagt ttctttaaat aacgcgtacg aatcatcatc atatttttca taatcatctc
     1441 ttgttattac tgtcgaactg acatcatcta tatcaccatg cattttataa acaattgtat
     1501 ccacatttgg attagttgtt gctaactgag tataattttt cttaacctga gggtttttat
     1561 aagcttctcg gattgatttt tctattagat gatcataatt agttgtccaa taagtttcaa
     1621 taggtaaact tgctagtatt cgatgatttt cagatatttc taattcttca ccaaaattat
     1681 ccataagtat ctgattcaaa attcctcttg atccaccatt ttcattttta taatattgag
     1741 ccagagtaac taagtctgtt tcttttttag aatccaaccc gatttctgtt gcaggatcat
     1801 taagtagttc cggccaactt acatatcctt gtgacatcga taatcctgca cctgcaaata
     1861 ttgctgcatt tcctttttca atagcttcag agaacctctt tattaaaact ttcttatcca
     1921 ttctacccgg ttcctttcga ttcagtaaat
//
LOCUS       DAFGFZ010000229         1928 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000229 DAFGFZ010000000
VERSION     DAFGFZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.236"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..367
                     /locus_tag="LR726_003002"
     CDS             <1..367
                     /locus_tag="LR726_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL6218009.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="LR726_003003"
     CDS             567..770
                     /locus_tag="LR726_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218010.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1330..>1928
                     /locus_tag="LR726_003004"
     CDS             1330..>1928
                     /locus_tag="LR726_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL6218011.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          625 a          293 c          454 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggttcacct
      661 tagggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DAFGFZ010000230         1903 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000230 DAFGFZ010000000
VERSION     DAFGFZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.237"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            213..779
                     /gene="amaP"
                     /locus_tag="LR726_003005"
     CDS             213..779
                     /gene="amaP"
                     /locus_tag="LR726_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL6218012.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            792..980
                     /locus_tag="LR726_003006"
     CDS             792..980
                     /locus_tag="LR726_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL6218013.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            993..1550
                     /locus_tag="LR726_003007"
     CDS             993..1550
                     /locus_tag="LR726_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL6218014.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1582..1824
                     /locus_tag="LR726_003008"
     CDS             1582..1824
                     /locus_tag="LR726_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL6218015.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          691 a          299 c          398 g          515 t
ORIGIN      
        1 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
       61 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      121 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      181 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      241 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      301 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      361 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      421 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      481 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      541 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      601 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      661 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      721 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      781 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      841 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      901 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
      961 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1021 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1081 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1141 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1201 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1261 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1321 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1381 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1441 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1501 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1561 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1621 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1681 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1741 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1801 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1861 aaatttgaga accgtagcga cactcgctgc ggttcttttt tat
//
LOCUS       DAFGFZ010000231         1897 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000231 DAFGFZ010000000
VERSION     DAFGFZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.238"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(341..1864)
                     /locus_tag="LR726_003009"
     CDS             complement(341..1864)
                     /locus_tag="LR726_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL6218016.1"
                     /translation="MDPTVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYAQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          532 a          387 c          305 g          673 t
ORIGIN      
        1 ggttacatgg tatctgaaac agctaaaacc ttgtcattac agcaaaaaat cactttatct
       61 ccgtttcatt tcagatacat ttcacttaag cggattccaa aaaaacaaaa gaaaaaggaa
      121 cccctagtta gcctatgctt tttattggtt cctctcaaaa cacggtcata taacaacggg
      181 gtggggtaaa tgaatgaaaa aggtggagga aacattattc aatagctgtc ttaaatactt
      241 cgttataaaa tgtgaacaaa taaaagacat ctatccctat tttctacact tccactttac
      301 taccatcttt gaatcgtaca attttcttaa ctcttctcgt ttacctcaat tcattttcaa
      361 gtggaatcag ctcatcatct aacattttac gccatgctcg cttcatttct tgtgcgtatt
      421 taaatggctt cgcttctgga tggttggctt tatatccttc gtaatcctta ctgttgataa
      481 ttgctttcat attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta
      541 attctttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca tcactcgctt
      601 gtgtaaaata gcgacgttta atgacaaaga tatcttcatt actatctaaa gtccccacat
      661 caaacgcatt taaaatttgt cggtaaactt ctttgactcc ttctgtttta ttcaatttag
      721 ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt cgccgcataa gttcctagta
      781 attgatcaat ataagtagaa tcaatatcat acaacttcgt ggtattttca ctataaaatt
      841 cgatcgtgga aaaatcttct ccctcttttt ctggttcttc ctctattaat gaacccttca
      901 cggtctcgta cacgccaatt tgttcttcca aaagttgaat ttgattgttc aaggttgaag
      961 atttcaccat gtcatcattg taatcgtcat aagtcactaa cgattcataa gcacgattca
     1021 attcttggaa tgctttgacg tactcgatac ggatttcaag tggcgtatgt tcatcaatgg
     1081 attccatgga tgaaacctct tttaacttac catatgcttg cttgaatctt tttttggatt
     1141 cctgataact tggataaatc aaacccgatt cttcttttgc ttctgaatac agttttgtcg
     1201 catcctcgac attcttctcc attgtggctg gctttcggaa agtaacaatt agtccttttg
     1261 ttttttctgg atacgtacgg ttcgtacgtg aaaaagcttg gatcaatcct gcatactcta
     1321 gattacgatc cacaaatagg gtttgaatcg taggtgcgtc aaaacctgtc aatagacgat
     1381 ccactacgat cactaaatca atttgtttcc caaattgttt gaattccgct ctttttcgtg
     1441 ccaaacgatt attgatgtct ccgttataac gctcaatgtc tgctaatgac caggaagtat
     1501 cataatactg attatattcc tcaataattt tttgcatttc ttcttgttgg acacttgaat
     1561 ctcggctgtt ttcttctaat gaataggtaa tagcgattcg tggaaaatca ggatcctcca
     1621 tcgttcttcc ttcacgaatg ggctgattag gaaactcttt tgttaaccag tcaggttctt
     1681 tcgtcatttc tttgatcgct cgataatact ttttggccat gtcaatggaa ctcgtggtta
     1741 agatcgcaga cttcgttgga cggccatttc gaaaatcaaa ttttgtatag gcattgtctg
     1801 gacggaacat tttatgaatg actttttgga tatgttcatc tgcttcaaaa acagtagggt
     1861 ccaaataact ttcttttttg acaggctcca tttgatc
//
LOCUS       DAFGFZ010000232         1889 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000232 DAFGFZ010000000
VERSION     DAFGFZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.239"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            149..1294
                     /gene="tgt"
                     /locus_tag="LR726_003010"
     CDS             149..1294
                     /gene="tgt"
                     /locus_tag="LR726_003010"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL6218017.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1391..1771
                     /gene="yajC"
                     /locus_tag="LR726_003011"
     CDS             1391..1771
                     /gene="yajC"
                     /locus_tag="LR726_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL6218018.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
BASE COUNT          649 a          313 c          415 g          512 t
ORIGIN      
        1 tttctttttt ttgttattca taaaaaaagg ataaaaaaaa gaatattaaa taaagtagaa
       61 ggaagccgag ggaacttgca aaaagcactt gaaacgatta tgatggtggg agttgtcaag
      121 atattttata tagaaagtag ggaaatgaat gacgcaacct gccattcgct atcgtttaat
      181 caagaaagaa aaacatactg gcgcgcgttt aggggaattg atcacgccac acggaacttt
      241 ccccacacca atgttcatgc cagtaggaac attagctaca gtaaaaacaa tgtccccaga
      301 agagttgaaa gaaatgggtg ctggcgttat tttaagtaat acatatcatt tatggctacg
      361 ccctggtgaa gacttagtag aagaagctgg cggactgcat aaatttatga actgggatca
      421 accgatttta acagattcag gcggctttca agttttttca ttaagtgata tgcggaatat
      481 cgaagaagaa ggcgtgcatt tcaaaaatca cttgaacggt tcgcgtatgt ttttatcacc
      541 agaaaaagca atcaacatcc aaaataaatt gggctctgat atcatgatga gttttgacga
      601 atgtccacca ttcgatgaaa gttacgacta cgtgaaaaaa tcagtagaac gtacgtctcg
      661 atgggcagaa cgaggattga aagctcatgc taatccagac cgtcaaggat tatttggaat
      721 catccaagga gcagggtttg aagatcttcg tcgtcaaagc gcgaaagatc tagtcgggat
      781 ggatttccct ggttactcaa tcggcgggtt atctgtcggg gaacctaaat cagaaatgaa
      841 tcgtgtatta gaattcacta caccgttgat ccccgaaaac aaaccacgtt acttgatggg
      901 tgtcggtgct gctgattcat tgatcgatgg tgttatccgt ggagtagaca tgtttgactg
      961 tgtcttgcct actcgaatcg ctcgaaatgg cacatgtatg acttctcaag gccgtctagt
     1021 agtgaaaaat gcgaaatatg cccatgattt ccgtccaatt gatgagaaat gtgattgtta
     1081 tacatgtaaa aactatacac gtgcctatat ccgccatttg atcaaatgtg atgaaacatt
     1141 tgggattcgt ttgacttctt accacaattt atacttcttg ttgaatctaa tgaaacaagt
     1201 acgtcaagca atcatggatg ataatttgct tgaatttaga gaagcctttt ttgaagaata
     1261 cgggttcaat aaagaaaacg caaaaagctt ctaaaggaaa atcttttaaa aattaagtaa
     1321 aaaactagtg ttatcagcat ttttctgcta aagtgttttt tgtagataca gaattagtga
     1381 ggtgaaaaaa atgggaacat taccaatgct tgtgatgttt atcgcgctat tagcaatgtg
     1441 gttttttatg tcacgttcgc aaaagaaaca acagcaagaa cgtcaaaact tgttagatag
     1501 catgaaacca ggtgattcag tcgtaacgat cggcggatta catggagttg tttcagaaat
     1561 cgacaatgac aaacggacag ttgttttaga ctgcgaaggg atcttccttg agtacgatcg
     1621 tgcagcaatc aaaacggtca aaccagggac tgtcgtaaca aatgatgcaa cgactactgt
     1681 tgaaacaaca gaagaaccag ctgagacagt tgaagtaaaa actgaaacaa cagaaacgct
     1741 ggaagagaca gaagagaagg aagaaaaata agtatcaaag cttatttaaa gacgaggtag
     1801 ggcagaaaga tgtcctacct cattttttat tcaaaaaaga aagagataag agcatgggat
     1861 atattgctca atattgaata atcgggtgt
//
LOCUS       DAFGFZ010000233         1843 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000233 DAFGFZ010000000
VERSION     DAFGFZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.240"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(408..905)
                     /locus_tag="LR726_003012"
     CDS             complement(408..905)
                     /locus_tag="LR726_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL6218019.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(945..1754)
                     /locus_tag="LR726_003013"
     CDS             complement(945..1754)
                     /locus_tag="LR726_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL6218020.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          529 a          345 c          325 g          644 t
ORIGIN      
        1 aggttcgagt cctacaaggt acataagagg ttgtgaaaaa gctgccctgc atcaagtaat
       61 aagaacagcc aagtaaaaac agcttcttat gtttttactt ggctgttctt attactgcag
      121 atgcaagctt ttgaataccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt
      181 attagcttaa caaacggaaa aatagttcct catattttgc cgtttgttaa gctaataccg
      241 agggtcagct gattgaacac catttaagaa agaggtcgtg agcctgccgt ttcactctgt
      301 gtcataaaaa agccaatcta ctttgataga aaaagcacgt aggactaaaa cagttctgcg
      361 tgcttttttt attgtgtgtt aaatatcgta aatgaagata gatattttta tgattcgacg
      421 atagagacgt tgaaatcttt caacagctct aaagcggatt ctccgtgttt tctactggag
      481 actaacattg gatcaacaaa aaagtctgct tcaggaaaag cactctgcag gcctatcaga
      541 ttagagagta cacacaagtt tgtttcaatc ccgactactt cgatctcttt ttgttcttct
      601 ttgctttcaa ataaagtttc ctggatttca gctaaagttt caggtggaat cgtaaagtaa
      661 tactttttaa tcactcgttc attttcttgt ggagaaagaa gagggatcaa ttgcaaatct
      721 tctcgttctt cttctttgtc cttcagttca actggtatat cccgtgtgaa gagaatatat
      781 tcattatttt ggcgtgcttt agctaatctt tgagcaatac gttctgccaa ttgatctgac
      841 tctggaatat aaaattcgct gtttggatca agaatatgat tttgcatatc aataataact
      901 aacattttct ttcctcctac attcatatcg tcattttatt ctttttaata gaagtttttt
      961 aaaatataag caacaccagc ttcttcattc gtcagtgtta cttggtttgc aatttctttt
     1021 acttgtcgct gtgcattccc catcgcaata ctcaacccgc tgtaattcaa catagcaatg
     1081 tcgttttctt gatcgccaaa agccatgatg tcactcgatt cgattccaag gtaatccgcc
     1141 agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg tgatttctaa gaaatgcgga
     1201 tcagagaata ctgattggat accatctgct tgcagatctc tttgtagctt ctgtagtttt
     1261 tcttcactct tatctgttaa tgctaattta agatagctaa gagaaggatc ttccaacata
     1321 tttaaaattt gatcaaaaga atggttctgt aaattgaaat gttcataata agggtcatct
     1381 ttgactagat ttgattcatc gaatgtcagc tgatcatctt gggtttctcg gctagcaatg
     1441 atggatattt tcgcttcttg agcatagaag aaggcttttc tcagtgtatc gctatctatt
     1501 tttgaacgtc tgagaacttt cgccattttt ccatccttca tttcagcaat cagtgaacca
     1561 ttggaagaaa tcaaatagcc accgattcct aattcggaaa aaatatcttt agcagaaatg
     1621 ctgttccttc cggtagcgac ggttacgatt ccgccatttt catcaaattt ccgaagtgct
     1681 tggatatttt cttctggtat ctttccattt cgatcaagca aggtaccgtc taaatcaatc
     1741 gctgctagtt tcattttatc ttcctacttt ctttttacat atctattttc ctgttacctt
     1801 aagattaaat gagaatttaa aaaaatgaaa ctaacttgct ttg
//
LOCUS       DAFGFZ010000234         1793 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000234 DAFGFZ010000000
VERSION     DAFGFZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.241"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(456..1259)
                     /locus_tag="LR726_003014"
     CDS             complement(456..1259)
                     /locus_tag="LR726_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL6218021.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          574 a          337 c          217 g          665 t
ORIGIN      
        1 aacaagatat ttattttagc tatttcagtc atcatgaaag actaattagc aataaaataa
       61 ctaatattat ccatttatca gacgaaaaga aaaaacttat ctaattactc tatattaact
      121 tttaacaaat agttgacttg tccaaaaatg ttaccttcaa tctcacaaag atattaagat
      181 gtttcctacc aaaactgata tcaccggact ttttattctg tgttgtccaa aagtgttacc
      241 tttagtcgat caacaaatta aaaaaattgt ttttttcttg acttgtccaa aagtgttacc
      301 catagttgtt aaaatttgta tagattagcc attctttcaa atattattta gactgaaact
      361 tgcttacaaa aaaacttctt gcatttattg tccaaaagtg ttacccatac ttttttaaag
      421 aagcaggttg tattaaaatt acaacctgct tcttctcaat cttcttctaa ccaattatgc
      481 attgaaatgg ttggcaagtt tgattctggc tcactaaaag tgaatctgag ccgatcaact
      541 cttcttcctt tctttgcata gattttttca taatcaaaag tttcaaatac ctgtattccg
      601 ttttcatcag gagctaaaaa ttcttctttc gcctgctcta gtattctttt gtcaatattt
      661 gtcattctat aacttgaagg tatacttaat gctcttctaa aatcttcaac tttgactacc
      721 caataaccat ttcgccactc tttatttctg tattgcatga gatgacgata caattctttt
      781 gtgtagctac ttcttagttt tgtaaaatta tcaagttcaa aacgagtaaa atttttgcca
      841 attgagttta agataaattc aaattcctca tttgccttaa ttgtaactgt ttccttctgt
      901 ttatctatcc ggaatttttg aaaaagccac agctgttcat agacgtctga atcttcaaaa
      961 cggtaggtta aagtacctag tttgtcactc aaagctttta atacagaata aaaactgtcc
     1021 ttttctcttc tgtcgtattt gaccaaataa cgaaagaggt tgaaatcaaa agttacttct
     1081 gctgttcctt gacgtttcat ttttgataaa atagcccaaa acacatccat ttcaacaggc
     1141 ttaaatcctc gcaaagctgt tgtattcaga ccattgttat aacgcacaat tgcatttgtt
     1201 tgttctgata ctaaatctac atcactatca ataatctttt cggtaacttc ttgagacatt
     1261 gattactctc caatcttctt tttgcgctcc tttaatcatg ttaatctggt actaattaaa
     1321 ggaaacattt ttttctgctt gttacctaaa ttaaatcaca actaaaggta acagtcaaca
     1381 aagatttgtt actaatctaa ttttctattt tttcgccttc caaatcaaga aaaccgaagg
     1441 taacgtctta tcctagtatg atacctcaaa tgtccacttt tgttaccttt gctgtccaaa
     1501 agtgttaccc ttactgtcca cttttgttac ctttactgtc caaaagtgtt acctttatac
     1561 ccctttaata cttactctcc caagagatta agcctcccct aacagatata ttaacagata
     1621 tttaacagat atattaacag atatttaata aaggaggatt actcctctct tatttcaaaa
     1681 tcttaaaaaa aaactaacac tatctaggag tttattttgc ttgtgtaact ttgaataatt
     1741 gaaaaaaagg aaaaggagga agcttcagct tcccctttta tttatattta taa
//
LOCUS       DAFGFZ010000235         1765 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000235 DAFGFZ010000000
VERSION     DAFGFZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.242"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            240..596
                     /locus_tag="LR726_003015"
     CDS             240..596
                     /locus_tag="LR726_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL6218022.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            606..1505
                     /locus_tag="LR726_003016"
     CDS             606..1505
                     /locus_tag="LR726_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL6218023.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          596 a          296 c          357 g          516 t
ORIGIN      
        1 tttatagtat tttttgttat ttaagaaagg aataaggtta aacaaataac aggttgcttt
       61 ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga
      121 taagagaaat cataataaac gaatgactta ctttccaata aattgagaac agtcagaacc
      181 agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag gtgatacaga
      241 tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta gaagcagacg
      301 atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc caagtcagtt
      361 gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa acagatctaa
      421 gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa gacagtgcaa
      481 tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat agtttacaat
      541 taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa gactagaggg
      601 ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat aaattcggcg
      661 aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact cggcttcatc
      721 tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca
      781 ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat gtgcccaatg
      841 aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat attgctttaa
      901 atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt tccgaattga
      961 aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc
     1021 gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag
     1081 accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt cctaaacggg
     1141 catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat gcatttttag
     1201 gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa gtagtttttg
     1261 gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac agctgcagac
     1321 cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa gaacagttgc
     1381 tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac ccagcttttc
     1441 aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca atcgttccgt
     1501 attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa ggataagtaa
     1561 gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata atcagcgaat
     1621 gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt gtattctttt
     1681 tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatttaatt ataattaata
     1741 ttgattgaag tctaggcagt caagt
//
LOCUS       DAFGFZ010000236         1743 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000236 DAFGFZ010000000
VERSION     DAFGFZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.243"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..722)
                     /locus_tag="LR726_003017"
     CDS             complement(<1..722)
                     /locus_tag="LR726_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL6218024.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="LR726_003018"
     CDS             complement(727..1494)
                     /locus_tag="LR726_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL6218025.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
BASE COUNT          550 a          280 c          297 g          616 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 ata
//
LOCUS       DAFGFZ010000237         1734 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000237 DAFGFZ010000000
VERSION     DAFGFZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.244"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1394
                     /locus_tag="LR726_003019"
     CDS             <1..1394
                     /locus_tag="LR726_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL6218026.1"
                     /translation="KVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYE
                     GVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPAL
                     PEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPE
                     GQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSD
                     EVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVD
                     VSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNA
                     EVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNK
                     KDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTL
                     GLLGYKNRKKENE"
     gene            1528..>1734
                     /locus_tag="LR726_003020"
     CDS             1528..>1734
                     /locus_tag="LR726_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL6218027.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNS"
BASE COUNT          686 a          255 c          367 g          426 t
ORIGIN      
        1 aaaaagtaga agttgggggc acagtagatc taacagataa tgttactaac ttgccagcgt
       61 taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat accaacacac
      121 caggtaatta cgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa gatacggtga
      181 aagttccagt agaagtgaca gacaatcgtt ctgacgccga taaatatgaa ccaacagtag
      241 aaggcgaaaa agtagaaatt ggaggcacag tagatttaac agataatgtt actaacttgc
      301 cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca attgatacca
      361 acacaccagg caattatgaa ggtgtcattg aagtaacgta tccagacggt acgaaagata
      421 cggtgaaagt tccagtagaa gtgacagaca atcgttctaa tgcagataaa tacaatcctg
      481 aaggacaaaa agtgactaca gacttaaata aagagccaga tgcatctgag ggaattaaga
      541 acaaagaaga tttaccaaaa ggaactactt atgcttggaa agagaaagta gatgttagca
      601 cagctggaaa taaaaaaggt actattgtag tgacatatcc agatggatca tctgatgaag
      661 ttgaagtaga cgttacagtg acagatactc gttccgatgc agataaatat aatcctgaag
      721 gacaaaaagt gactacagac ttaaataaag agccagatgc atctgaggga attaagaaca
      781 aagaagatct accaaaagga actacttata cttggaaaga aaaagtagat gttagtacag
      841 ctggaaacaa aaaaggtact gtagtagtaa catatccaga tggatctaaa gaagaagtag
      901 aagttactat ttctgtagta gataaaaaag caccgaataa acctcaagtt gatcctatta
      961 cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg acagttacat
     1021 taccagacgg aagtcaacac catgggacag ctgataaaaa tggtaatttt acagttaaag
     1081 ttcctaaatt agaggcaggg acaaaagtga tagtaactgc aactgatgaa tctggtaata
     1141 ctagtgaacc tactaatgta gttgtttctt ccaacaaaaa agacagtgga aaaaacggta
     1201 gtaaaggtag taagactgac aatcaaggta gcaacagcaa tcaagacaaa aatagaggca
     1261 aaagccagtc ctcaaaagtt tttcctaaga cgggagagag tgacagtaat atctttacta
     1321 ttagcggtgg tttaatactg ttaggaacgt taggattatt agggtataaa aatcgaaaaa
     1381 aagagaatga atagaaaatc aatttcttaa gtcttctctg agaagaggaa gaatcttttt
     1441 cttcctcttt ttgctttttt atgatgggaa ataagtgtta gttaaagatg aatatacttt
     1501 ttatagaaag gaaaagaatg taattaagtg gaaaaaatag taattattgg ttcaaaccat
     1561 tcagggctag ccgcagctcg atttctaaac ttaagcgatt caaattatga agttattcta
     1621 atcgataaaa aatcagaaat tagttacttg gaatgtggga catcgttatt tataaaagga
     1681 gaagtaagta atttagaaga tcttttttat caaaatagaa tgacactaaa ttct
//
LOCUS       DAFGFZ010000238         1717 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000238 DAFGFZ010000000
VERSION     DAFGFZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.245"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            280..894
                     /locus_tag="LR726_003021"
     CDS             280..894
                     /locus_tag="LR726_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6218028.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(918..1268)
                     /locus_tag="LR726_003022"
     CDS             complement(918..1268)
                     /locus_tag="LR726_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL6218029.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1262..1525)
                     /locus_tag="LR726_003023"
     CDS             complement(1262..1525)
                     /locus_tag="LR726_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBL6218030.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          579 a          300 c          256 g          582 t
ORIGIN      
        1 ttttcttaat aaatcttaac agataaatca agttatatac tagaaaattc ataaaatata
       61 tttttaatgt cataatcgtt taaattttct tgattgacat ttatttacaa aaaatattgc
      121 catttaaaaa gaaaagtgac acatactatt tcctccatca ttagtttttg tctatataag
      181 gtatacccta actagacacg accgcgacat tgaaaaatct ggttttctcg tgtatgatta
      241 gggtgttttt taaatagttg cagaaagtag gtaaaaattt tgagagttgc atacatacgt
      301 gtctcatcca tcgatcaaaa tgaacaacgg cagattgaag aaatgaaaaa attcggtgca
      361 gagagaattt tcattgagaa acaatctggc gcaacgatta ctcatcgact tgtttttcaa
      421 gaagcactgg atttcgtccg agatcaagat atttttattg tggaagctat tgatcgactt
      481 ggaagaaatt acgacgagat catcgcttca gtcaattact tgaagaagaa aaatgtgcag
      541 ctgatcatca caagtctacc gatcatggcc gaagcaattg gcaatcctct cctggataaa
      601 ttcattaaag atttgatcat tcaaattttg gccatgatcg ctgaacaaga acgtaccgaa
      661 tctaaacgac gtcaggcaca aggcattaag attgcaaaag cgaacggtgt ttataaagga
      721 cgtcctaagc tatacagtgc caatgcaaaa gatccccaac gtcgtcttgt ttataaaaac
      781 atcgttgagg atctaaaaaa aggtgttgcg atcgctaaga ttgcaaaaga ctacaatgtt
      841 acgagacaaa ccgtatatag aataaaaaaa gacagcatgg tcaatcacga atagttgatc
      901 atgctgctgt ttctatttta tttgcttact ttttgaaaaa ctgttctaac tttcatcaat
      961 aactcctcaa ctaaactatc atgcgctttt tccaaaaaaa tacactctct ggcttcataa
     1021 tcaattgtcg ttagttgatc taataatact tttcctgttg tcttcatttc atattctgag
     1081 tttatattta catatagcgg atagtttcta gttgtactag aaattggagc aactgtaaca
     1141 acgttactat aatccgcaat aatatcatga cttaaaacaa tataaggtct tttacctgtc
     1201 tgttcgtgtc cagatcgagg tgctgtattc aataatagaa tatcgccttg tctaggtttt
     1261 ctcaccattt ttcattacct actgcttcac ctaaattttg aatcgtcaca tgatttcttt
     1321 catcagtgta attttcaaat aactgttcaa ttgttaattc attttctata ttagccgctt
     1381 cgataattag ccgcttctga ccgtttactt cttcttgtat tatcttcact ttgtcttctg
     1441 tatgaattcc aagatattcc ataatatact ttggcaatct aaggccatta ctatttcccc
     1501 atttttttat aggtaacatt tccatagtag acacctcttt ctgtatatac atagtataac
     1561 atacataaga aaaaacgtat atacttaatt atccttttct attaaattag attaacattc
     1621 catgaatcca taaactatgt taacatttat atgaaaatag tcgaaaggaa atcataatat
     1681 gtttaagaaa acaaaatctt tagaatcagg taagaaa
//
LOCUS       DAFGFZ010000239         1702 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000239 DAFGFZ010000000
VERSION     DAFGFZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.246"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(130..1278)
                     /locus_tag="LR726_003024"
     CDS             complement(130..1278)
                     /locus_tag="LR726_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6218031.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1295..1699)
                     /gene="tnpA"
                     /locus_tag="LR726_003025"
     CDS             complement(1295..1699)
                     /gene="tnpA"
                     /locus_tag="LR726_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL6218032.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          499 a          293 c          273 g          637 t
ORIGIN      
        1 ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat agcgtatttc
       61 tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta cggttcttga
      121 tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca gaatattgat
      181 actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc attctcgaat
      241 ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac tagacggaaa
      301 ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt gtaatttagt
      361 cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat ttcgcaacat
      421 accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca tttctgtact
      481 caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc tagctacttt
      541 gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat ttttagccaa
      601 taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca ttttggacgt
      661 gaatttatta ttatcgattt tttgtccatc agaaagaatc gcaaagtcca taatacctaa
      721 gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt tacacaacaa
      781 agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga taatcccttt
      841 tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt tgatgaattt
      901 actatcaatc aaagcaactg taccattttg attattcgtt gtataactct gaacaggatt
      961 tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc
     1021 tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt tcaaaaacgg
     1081 aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag aatcattttt
     1141 aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct tacggacaca
     1201 accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc tgaatttgta
     1261 tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt ccttgatttt
     1321 ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga caaaagcttt
     1381 tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca cgttttatca
     1441 atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt ttaggttgag
     1501 ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa gtaatgtgat
     1561 acgattctga aattctttca aaagtagttt tggcataatc agatatttca tcatcaatca
     1621 cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat actgaatgat
     1681 tatttgtatc taattccatg tt
//
LOCUS       DAFGFZ010000240         1698 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000240 DAFGFZ010000000
VERSION     DAFGFZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.247"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..568
                     /locus_tag="LR726_003026"
     CDS             <1..568
                     /locus_tag="LR726_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218033.1"
                     /translation="YDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPL
                     LSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLEN
                     DFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCE
                     HYNRLSEEKILLPENWAELRKLVEYGII"
     gene            555..>1698
                     /locus_tag="LR726_003027"
     CDS             555..>1698
                     /locus_tag="LR726_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL6218034.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIP"
BASE COUNT          565 a          218 c          269 g          646 t
ORIGIN      
        1 ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa
       61 aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt
      121 tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt
      181 aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac
      241 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
      301 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
      361 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
      421 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
      481 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
      541 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
      601 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
      661 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
      721 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
      781 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
      841 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
      901 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
      961 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
     1021 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
     1081 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
     1141 taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat
     1201 acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac
     1261 aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct
     1321 acattagtaa cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg
     1381 ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta
     1441 actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt
     1501 tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa
     1561 ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga
     1621 aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa
     1681 acatgatttt cataccta
//
LOCUS       DAFGFZ010000241         1689 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000241 DAFGFZ010000000
VERSION     DAFGFZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.248"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            115..459
                     /locus_tag="LR726_003028"
     CDS             115..459
                     /locus_tag="LR726_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218035.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            495..890
                     /locus_tag="LR726_003029"
     CDS             495..890
                     /locus_tag="LR726_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL6218036.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            943..1254
                     /locus_tag="LR726_003030"
     CDS             943..1254
                     /locus_tag="LR726_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218037.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            1266..>1689
                     /locus_tag="LR726_003031"
     CDS             1266..>1689
                     /locus_tag="LR726_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBL6218038.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMN"
BASE COUNT          702 a          234 c          294 g          459 t
ORIGIN      
        1 agcttattaa attagcttac gagttaaata taagacaatt ggatagattt gtcaactacc
       61 tttctatctt tttgtataaa ttattatcgt acgctctagg aaggttgatt tgacatgctt
      121 ttcgaaaggg ttaaagagct ctgtaaaaaa agaggtattt ctatttctga attagaaaat
      181 agaataggat ttggaaaaaa ctctatttat aaatggaaga cacaaagtcc taaaatagaa
      241 actttgcaaa aagttgctga ctacttccat gtatcaacag attatttgct aggacgcaca
      301 gatgatccta acgcgggagt tgcaccagag gaaagaaaac taaccgtgga agaagcttta
      361 gcatctgtta tgagtagcga cggaaaaccg ctcaccgata atgataggga aatactatca
      421 gctatgattg aagcatattt agagaaaaaa gataactaaa taagtaggtg agtcatttgg
      481 acagtcaaat tgaaatgata attaatgaac tcggcgttaa ggtagaagag cgtgaaaacc
      541 ttgatgccga tggccattat gttgcttgta tgaataccat agtaataaaa gctaatttat
      601 ctaagtatag aagacaaaga accttattac atgaattagg acacgcttct aaacatcatg
      661 ataattattt tttatataac ttagcattct ctctccattc aaaaatggaa tatgaggctg
      721 atcgcttcat gattgaaaaa ttattagata gatatattgc aaagtctgaa ttagaaccac
      781 acaatatcaa ttacatgaaa tttatagaag ataacaattt aagcgttcgc ttcgaaccac
      841 ttgtgaaaga attattaaaa gctcgcatct attgttatgc agctctctaa aattttttaa
      901 gcaaaaaaga acatacgttc aaaaatagaa aggtgaacaa aaatgatata tacagaattc
      961 aaagaatggt tagaaaaaag cacaaccgga tacgaaacat ttatcatcaa agctactaat
     1021 tatcaaattg aaaaaaacaa aaatagaccc caaaaaaaac gctgggatga taagaaaata
     1081 gataaagctg tattagaaat gtggaaacaa gtcgtgacta acttgtatca aacaattcgt
     1141 aaagaaaaag gagttccatt aattaacggg aaggaaatat ggcttgaatt tatagaggaa
     1201 caaggactga tcgaattttt caatgatagc atggcagaat tagaatttga ataggggtaa
     1261 tattgatggc aatgataaaa caatataaaa agaaaaatgg cgaaaaagca tggtacttta
     1321 aaacttatct cggtattgat ccgctaactg gaaagaaaaa atatactact aaacgaggat
     1381 ttagaacaca aaaagaagca aaaacagcac tttctaggtt agaactagaa ttacaaaaaa
     1441 caggaatgcc cacaagtaca aatactactt tcaaagaagc agcagaatta tggctagaaa
     1501 gctacaaaaa aactgtaaaa gaaagttcat attcaaggac taaaataatc tttaataaac
     1561 atatatatcc caaatttgga aatattaagc tttctaaaat taatacggca tattgtcaaa
     1621 aggtagtaaa tgattggagt gaaaaaggaa cttcaaagca gtaccctctt ttcataaact
     1681 atatgaata
//
LOCUS       DAFGFZ010000242         1672 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000242 DAFGFZ010000000
VERSION     DAFGFZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.249"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            5..403
                     /locus_tag="LR726_003032"
     CDS             5..403
                     /locus_tag="LR726_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL6218039.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="LR726_003033"
     CDS             387..1082
                     /locus_tag="LR726_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL6218040.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          630 a          246 c          328 g          468 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tt
//
LOCUS       DAFGFZ010000243         1633 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000243 DAFGFZ010000000
VERSION     DAFGFZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.250"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(592..>1633)
                     /locus_tag="LR726_003034"
     CDS             complement(592..>1633)
                     /locus_tag="LR726_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBL6218041.1"
                     /translation="AIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVENL
                     SIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDLV
                     DSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLIV
                     HSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFSA
                     SFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGGA
                     TFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRDV
                     INDDSKVIDVNA"
BASE COUNT          513 a          357 c          289 g          474 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
      361 cttgcatctg cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg
      421 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag
      481 aagtagcttc tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa
      541 acttcttctt atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt
      601 cacatcgatc actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc
      661 acttgtcacg ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc
      721 gattaatggt tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc
      781 taaaacgatc agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact
      841 gattgttaca attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc
      901 tgctggtgcg acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc
      961 aatagacaaa ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt
     1021 tgtctgagtc tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa
     1081 gctcaatacc ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa
     1141 aatcagtgag tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt
     1201 acctaaattg acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa
     1261 tactccataa ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag
     1321 actgtcgact aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag
     1381 ataagcgatt ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact
     1441 acctgctaaa tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga
     1501 taagttttca actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc
     1561 tccttgtgtg aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa
     1621 tgctgcaata gct
//
LOCUS       DAFGFZ010000244         1626 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000244 DAFGFZ010000000
VERSION     DAFGFZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.251"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..548)
                     /locus_tag="LR726_003035"
     CDS             complement(<1..548)
                     /locus_tag="LR726_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6218042.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLAD"
     gene            complement(559..1227)
                     /locus_tag="LR726_003036"
     CDS             complement(559..1227)
                     /locus_tag="LR726_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL6218043.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1357..>1626)
                     /locus_tag="LR726_003037"
     CDS             complement(1357..>1626)
                     /locus_tag="LR726_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL6218044.1"
                     /translation="SAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEE
                     IHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          586 a          273 c          220 g          547 t
ORIGIN      
        1 aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc atccaatata
       61 attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg ttcaccactt
      121 gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc agctaattca
      181 ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga aaatactaac
      241 tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac gtagccaata
      301 ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa taaaatttcc
      361 ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt taatagtgtg
      421 gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt tattgaaaaa
      481 ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac attgttaaat
      541 tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa cggaaaacag
      601 gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt tcacaggaga
      661 ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag ttgcacgtat
      721 aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag acaagcgaat
      781 gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag tcggaaagaa
      841 tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt tttctaaaga
      901 ggataataat tcattgctag tatagacact aaccaacaac catgagatca ttacgaccgg
      961 caacattcga aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa actctatctt
     1021 gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga tgaaataaat
     1081 acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga aacaccccac
     1141 ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat ttaaagtaat
     1201 caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga tcgcactgtt
     1261 atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta tcaaaaagca
     1321 taactgagaa caattctcaa tgcaagcttt ttttaattaa ctcagtaatt caatccaccc
     1381 aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt ttgaatagct
     1441 atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt ggatttcttc
     1501 ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat ctagacaaca
     1561 gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca atatcttgat atttggttcc
     1621 agctga
//
LOCUS       DAFGFZ010000245         1626 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000245 DAFGFZ010000000
VERSION     DAFGFZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.252"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1394
                     /locus_tag="LR726_003038"
     CDS             <1..1394
                     /locus_tag="LR726_003038"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL6218045.1"
                     /translation="RELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQATFQ
                     LNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGH
                     DGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTM
                     GSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNK
                     DWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYH
                     LKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVL
                     KKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLANFDEIK
                     ENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENE
                     AWLQGALEVFKHE"
     gene            1387..>1626
                     /locus_tag="LR726_003039"
     CDS             1387..>1626
                     /locus_tag="LR726_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL6218046.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNEL"
BASE COUNT          567 a          273 c          344 g          442 t
ORIGIN      
        1 atcgcgaatt actttcagat gaagaatcaa aagaagatct gattgcaaca atcgttgata
       61 ttttggggta tgcgatttcg ccagagtatc ttttcaacgt cttagcggat caagcaaaac
      121 aagcaacgtt ccaattgaat gatctaaaca aagcctttgt gcaattggca tctacctaca
      181 atcaatttaa tgggttattt gatgatgtgg atctccaatc aaaaaaacta ggaacagatg
      241 agcaacaacg aaatgtgacg attaccgaag tcattaagaa attgaatgac gtggaagttt
      301 taggtcacga tggggacgtg atcggggatg cttatgaatt tctgatcagt caatttgctt
      361 cagaagcagg gaagaaagcc ggagaatttt acacgcctca tatggtttct gatatgatgg
      421 cacaaatcgt gacgttggat caaaaagaac gacgattctt tagtgttttt gatccaacca
      481 tgggttcagg ttcattgatg ttaaatgtgc gtaactactt aactcaccca gataacgtta
      541 aatatcatgg gcaagaatta aatacaacga catacaatct agcaaaaatg aacttgattt
      601 tacatggggt agatgccgaa gaaatgaatc tccgcaatgg ggatacgttg aataaggatt
      661 ggccaacaga tgaaccttac acatttgatg cagtagtaat gaacccacct tattcggcta
      721 attggtcagc agatacgacc ttcttagacg attctcggtt taatcgttat ggaaaattag
      781 cgccaaaatc caaagcagat tttgcgtttc ttcttcatgg tttctatcat ttgaaggaaa
      841 cagggactat ggccatcgtc ttgccacacg gagttttatt ccgtggcgct gcagaagggg
      901 tcattcgtca aaaattacta gaagacggca gtatttatgc tgtgatcggt atgcctgcga
      961 acttgttctt cgggacatcg attccaacga cggtgattgt attgaaaaag aaccgccaaa
     1021 ctcgagacgt attattcatt gacgctagtc gagaatttgt caaaggaaaa aatcagaata
     1081 aattatcgga agaaaacatt caaaaaatcc tcgaaaccta tgctgaaaga aaagatgtcg
     1141 agaaatacgc tcatttggca aactttgatg aaatcaaaga aaatgactat aacttaaata
     1201 ttccacgcta tgtagatacg tttgaagaag aagaaccaat tgatatggtt catgtcggaa
     1261 atgacatcaa gaagatacga caagaacagc aagtgctaga aaaagaactt ttagaagcta
     1321 tctcttccct gcaaacaaca cctgaaaatg aagcgtggtt acaaggtgcg ctggaggtgt
     1381 tcaaacatga gtaatgatat acaaccagaa attcgtttcc caggatttac ggaagcttgg
     1441 gaacagcgta agtttgaatg cttacttgat aaaaaagatg gagtaaggag gggaccattt
     1501 gggagtgcct taaagaaaga attctttgta tctaatagca attttgttgt ctatgaacaa
     1561 caaaatgcta tttatgataa ttacgaaaca agatataaaa ttactgaaaa aaaatataat
     1621 gaatta
//
LOCUS       DAFGFZ010000246         1588 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000246 DAFGFZ010000000
VERSION     DAFGFZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.253"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..760)
                     /locus_tag="LR726_003040"
     CDS             complement(<1..760)
                     /locus_tag="LR726_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBL6218047.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSY"
     gene            complement(919..1185)
                     /locus_tag="LR726_003041"
     CDS             complement(919..1185)
                     /locus_tag="LR726_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218048.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1175..1531)
                     /locus_tag="LR726_003042"
     CDS             complement(1175..1531)
                     /locus_tag="LR726_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL6218049.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          451 a          303 c          195 g          639 t
ORIGIN      
        1 agtaactcct taaattgtta tcactttccc attgaactac ttttctataa gttgaattat
       61 cagttgaaaa gatagaattt tcatccataa acgcatatat tagcagcaat ctttcatcat
      121 tgtcaaattc tattggatca aaaatatcta agttgtcatc aaaatcttta tcagttccgg
      181 taactttttt gttaattact gaaggtgtct tgtaagaaat agaatctaaa tcaaagcgtt
      241 ttgattctaa attattttgt gctttctcaa ttaaataatt cacttttttt gtgagaaacc
      301 ttctgatatt gtctttgcta tatgttccat aaaaaccctt atcttctaaa gagtccctgt
      361 acttcttaac ttctatatac tcttcagcca aaacttgtgg aactggttca tctaagaaaa
      421 aaacccctac ctcgcctttg ctatttttat aaaagtaatc aatttctgct atagttcccg
      481 atctataatt atcaactgga gaacccaact tatggccaaa aatcgctaat aatatatcac
      541 tattttctac aatttgttta ttaataacac tttgcccaga gctattattt atataagtag
      601 gaatcgcatt cgtttcccat cgaattggca tcaaaattac ttttgtatta tctgtatttt
      661 ctatattcca tctatatatg gtttcttcta cttcattcct ctttgctgct atatcacctg
      721 gtgatgctat aaatactttt attagattta tggttttcat ttattgtacc tttctcttta
      781 tgtctcttta aagtaatttt tgtataaaat ttattgactt ttacactata ggacaacttt
      841 tatctattta gtacaaaaag gcccactaaa ctaattgatt agttaggagc ctattacagg
      901 tatattttca actcgctttt aagcaaattc tactaccagc tttttgccta atgccctagc
      961 gatatcatca agtttatcga aagatacgtt atcccctctc tcaattcgag caatagtaga
     1021 ttgagttgta tgtgcttttt gtgcaagctc cgcttgagtt aggcctgctt cctcccgtgc
     1081 atgataaaga gcaaccgcag tttcgagttt tcttccttca cggtcatatg cttctgcaaa
     1141 atctttgtct ttttgctttt cttcaatcaa ttttttaatc atcatttgtg aaccactcct
     1201 ctctaagttc ttttgcatgt ttaagttcac ttattggtgt tttctccgtt tttttagtaa
     1261 atccatgggt aatgacatac cttccattga ccacatggaa atagatagct ctttggatgt
     1321 ttgatcctac cttacttctt aattcgtaca aattagtgtc taattttttc acccatttca
     1381 accgttgagc tactagcatc ccattctttt ctgtttcttc tattgtacgt aaaagcttcg
     1441 cactatcttt ttttggtaat gtttccaacc attctaaaaa ttcatcatga ccattaggtc
     1501 ttttaaatgt ttcaaaagat attttttcca tacccaatta tatcatatat tgattttata
     1561 ttatagtata taacataaaa attcgaat
//
LOCUS       DAFGFZ010000247         1582 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000247 DAFGFZ010000000
VERSION     DAFGFZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.254"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(22..399)
                     /locus_tag="LR726_003043"
     CDS             complement(22..399)
                     /locus_tag="LR726_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218050.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(699..1580)
                     /gene="galU"
                     /locus_tag="LR726_003044"
     CDS             complement(699..1580)
                     /gene="galU"
                     /locus_tag="LR726_003044"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL6218051.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          435 a          356 c          240 g          551 t
ORIGIN      
        1 tttgcgtata cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga
       61 atctgtacta gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt
      121 catatatggg atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc
      181 ttcccaacta cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata
      241 ccagataaaa atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact
      301 aataagcaat agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat
      361 atcaagcagc gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt
      421 ataattaaaa aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct
      481 cttatgtaat cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt
      541 ttctgaatct attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct
      601 agagtcaaaa aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac
      661 aaatagcctt tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt
      721 ttactcaact ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca
      781 tattcaatat ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta
      841 aactctctcg catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt
      901 atctcgtttc ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt
      961 agataacgtc cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca
     1021 aaccctttta ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat
     1081 ttagatgttt cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca
     1141 taatccatca tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc
     1201 atgacgacaa acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt
     1261 cctcttggat gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa
     1321 gcgagtaatt ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca
     1381 aagtgatctt caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc
     1441 gaatccaatg cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt
     1501 tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg
     1561 actgcttttt tgaccttcac tt
//
LOCUS       DAFGFZ010000248         1566 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000248 DAFGFZ010000000
VERSION     DAFGFZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.255"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            56..1522
                     /locus_tag="LR726_003045"
     CDS             56..1522
                     /locus_tag="LR726_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL6218052.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          434 a          291 c          330 g          511 t
ORIGIN      
        1 tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat agattatgga
       61 aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt tgttcttcac
      121 tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct attatatgta
      181 cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag ctatcatgtc
      241 gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat ctgaccgagt
      301 gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg gccacatcgt
      361 cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga ttattatcgg
      421 aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt caaagactga
      481 cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag gggcattatt
      541 agcaccacta atcgttggta caatcggtca agaatataac tatcatttag ggttctcttt
      601 agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac gcaagacatt
      661 agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta aaggattcat
      721 ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg cacaactaac
      781 tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga ttatcttacc
      841 gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag aaaaaccaag
      901 agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat tacaagaaca
      961 aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc tatttggtgt
     1021 aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta ttctgacacc
     1081 agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag tagtgaaatt
     1141 ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc cgggtatgct
     1201 ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct tcatcgtggt
     1261 tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat tagcaccacg
     1321 agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt cacaagcaat
     1381 caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact tcggtattat
     1441 cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac caatcaaaaa
     1501 actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt atttgcagca
     1561 gttcct
//
LOCUS       DAFGFZ010000249         1548 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000249 DAFGFZ010000000
VERSION     DAFGFZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.256"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(1..648)
                     /locus_tag="LR726_003046"
                     /pseudo
     CDS             complement(1..648)
                     /locus_tag="LR726_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(970..1191)
                     /locus_tag="LR726_003047"
     CDS             complement(970..1191)
                     /locus_tag="LR726_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218053.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1289..1534)
                     /locus_tag="LR726_003048"
     CDS             complement(1289..1534)
                     /locus_tag="LR726_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218054.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          447 a          304 c          236 g          561 t
ORIGIN      
        1 agtagacacc gaaaagccga agagcgttcc atttcttcgg ttctttttat atattcctcg
       61 aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg agactttgat aaaatttatt
      121 tcgtcgttta ataggtcgat ggtcttgttc tattaaattg ttaagatact tcacagttcg
      181 gtgctctgtc ttagtatata aacccacact ctgtaacttt ctaaaggcgg agccaagaga
      241 aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac tgtttatgga gtcgttttaa
      301 gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac cagatatcta aggttaagcc
      361 gtccgcatca attgcacgat aaagataatg ccaacgtccc ttaattttga tataggtttc
      421 gtccattttc catgaataga aggattgtct atttttcttc ttccaaagat aatagaggac
      481 tttgctgtac tcttataccc aacgataaat cgtagtatga caaacattta ttccacgatc
      541 atataacaat tcctgaactt cacgatagct tagattgtaa cgcaggtagt aaccaacagc
      601 gacaataatg acgtcttttt tgaattgttt gcctttaaaa tgattcatta ctctgtcctc
      661 tctgtctttt ttctcaattt tacactaaaa tagatttttt ggaaaacttt gcaacagaac
      721 cgtttttact atatacttct tcccagaact tgtccgtctc cttcaaatta tctctacctc
      781 tcgagttcat tgggtggttt gcccatttat taaacatgga atcacctttc tcatactcat
      841 atatttatgt cctatttcaa ttatatttat atatgcttaa ataaatttgt atcctgaaat
      901 ttattgtaat atgaagctgt attttacata tagtaaccaa agagttaata aaataaaaag
      961 agcgaaccat tatagatcag ctcttttttc attgctgttt ttaatcttat ctagaatcac
     1021 ttcaaatgtt tcaaaggtat ttacatctaa ctgacataac caaagcaagt acttcttttc
     1081 tttgtaagtc caatcaatat tacggtaaga ggacattaga ttatggtacc ttgctctttg
     1141 ttcaactgaa gccaattccc agcgtttttc aaatattttt tcttcttcca ttattgatcc
     1201 ctcgcaagac gctacctcaa acttaaataa tgggcagcga aagtctaatt ccttagatct
     1261 aagtatagca aacaaatatg aaaaaaactt agaaggtagt aaaagttctt ccgtcttttg
     1321 ctttgcttct attcttaatg tgtttcgtta tcggtgccct acaaagattt tcattaatta
     1381 cacactcatt tattgttgtg tattttctga catttctctc ttgatcaatt attttaattc
     1441 catgggcatc ctgatagttc ataggagagg agcggctatc agaaatagtt gcacctatct
     1501 cttgaacttc ttttacaact tctggaaaat ccatccatcc tgcttcag
//
LOCUS       DAFGFZ010000250         1543 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000250 DAFGFZ010000000
VERSION     DAFGFZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.257"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..239
                     /locus_tag="LR726_003049"
     CDS             <1..239
                     /locus_tag="LR726_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBL6218055.1"
                     /translation="QILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRE
                     LFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            259..1479
                     /locus_tag="LR726_003050"
     CDS             259..1479
                     /locus_tag="LR726_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBL6218056.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
BASE COUNT          531 a          279 c          311 g          422 t
ORIGIN      
        1 gccaaatatt ggattcacca gagttaaagg cacgaattat gccaagctca gaatttttat
       61 tgggtcattc actccaagcc tttgatgagc aaggacgact cattgatgaa gaacaagtcg
      121 caaaattacg tgaactattc aaagatttct tactctttgt cacaatcact agtcaattgc
      181 aacatgcgca ccaagcaaat aaacgtgaag ccgaaagctt ttcatgggaa acgatttaat
      241 cgaggggagc aaacaaagat gaaattaata ggtatcgtcg gatcagcagc tgaactttca
      301 tataaccgta aattattaac atttatccaa aaacaatatc atgaactatt taatttagaa
      361 atcattgaaa tcaaagattt acctctgttc aatcaagatg atgatcaaac aaatagtcct
      421 gctattcaac agctaaatcg caaaatctta atggctgatg gggtaattat tgctacgcca
      481 gaacacaatc atacggttcc agcaggattg aaaagtgcac ttgaatggct atcatttaaa
      541 atccatccac ttgaaaataa acctgtaatg gttgtgggtg cttcttacta cgatcaagga
      601 acttcacgcg cgcaattaca cttgcgccaa attttggacg caccaggcgt gaatgcatta
      661 gtgatgcccg gaaatgaatt tttattggga aaagcaaaag aagcatttga ccaagaagat
      721 cgcttgattg atccacgaac tgttgacttt ttaggcagta cattagcaag ttttgtaaaa
      781 ttcattaaag tcgtgtctac cttagcaggg cctaaaaagc ttccaccaga agatttgcat
      841 gcgacaggaa caattgctac gacaataccc gatgttgata tgtcagatga tcattggttg
      901 gaaaaagctt ctgaagcagt cggtgccgtt tctggaaata cttacgtcaa attaaatcgt
      961 gggttattga cagtggatca aatcaattac tttttagatt cgatgccgat ggaattaact
     1021 tttgcagata ataataacca atttttatat tataatttgc gccaagaagc gaaagagatg
     1081 ttagctgctc gtgtgcccgg tcaggtaggg aatccattag ccaactgtca cccagaaaaa
     1141 tcgtataaaa atgtagagtg ggtaattcaa caactgcgaa gcggagcaac agatgcggta
     1201 cgtgttcatg taccaaaaca agtgccagaa aaatatattg tgcataatta ccaagcaatg
     1261 catgatgaaa atggagagta tgctggaatc aatgaataca ttcttgattt caaaccaatc
     1321 attgagtggt atttacaaca aacgggtcaa caacttgttg gaaatgaaga tgcggtttca
     1381 agtgcatctg ttcatggtgg aaaaacagat gcagtaacca gcgccagcac acacgatgaa
     1441 actgcagtag atgcagtttc gagtgcttct gtaaaataaa aaggttctgt tgcaaagttt
     1501 taaataaaga ataaagtccc ttacggtatc tatgatttaa gct
//
LOCUS       DAFGFZ010000251         1519 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000251 DAFGFZ010000000
VERSION     DAFGFZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.258"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(832..1362)
                     /locus_tag="LR726_003051"
     CDS             complement(832..1362)
                     /locus_tag="LR726_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL6218057.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1359..>1519)
                     /locus_tag="LR726_003052"
     CDS             complement(1359..>1519)
                     /locus_tag="LR726_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL6218058.1"
                     /translation="IAFYFSAIIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLIL
                     ITKRDGER"
BASE COUNT          473 a          305 c          174 g          567 t
ORIGIN      
        1 ctatgaaaat atcttttacc tttctcctgt gaaaaggaaa tttccttttt tatagtgtta
       61 ttgatataat cagaatattc atcaaactct gtatctggag ttaaaaatct acagtaacat
      121 ctataagctt tgctagtaat cttcttgggt tctctctcca ttgtcaagca acatcaaaac
      181 acctacttac tattcgattc ttttctgcca aaaacgagtc agcatcactc acaacttatg
      241 tgttgctgac tccgactagt cctttgttca atatcaactt attaaacaga tataatattt
      301 ttctttattt cgtatactcc aagacttcca tctttctttc atcttatctc tgaaaaaaac
      361 acagcaaagt gcccttaata aatatacatt agaatttgca aattttttca tcttaagata
      421 tatcctattc atattcttta caatcctaat gggtgattac aaatctattt agttcaagtt
      481 ctacattata aacacctttc ttgatagtat catctgctgc acaagcgatg tcgatccact
      541 tattagggga tttacgtgct caaaagtggc tattcctttt gggaagtcca tccataataa
      601 tggtccatgg atttttttag ggtcttgtcg ttcaaatttc tcaaaaactt tattatactt
      661 ccatagtttt ataattattt ggtttcactt tggctcgttc aaacaccaac ttgtatttct
      721 tttcaacgtt cgaactggag ccccccctat aatctcatta ttattttcat gactttttac
      781 taccgtggaa ttacaagcaa caacaatatt atctgctaaa tgaacacctt tctatatcac
      841 gctttttgct ccaatccaac aatgtttacc tataactatt ttattatttt tattcaaaat
      901 cctatgagta ttcaattcaa aaatcgtatg tgaatcggta tccataaata atgtatccca
      961 cgatactgta cagccatctt tgaattgaat ttccttaaag caaattatac tcatttttcc
     1021 tgtcgaatta aactttgacc caatatctaa aactgcattt ttccttatgt caatatgagc
     1081 cccttgtgcg ataaacgctg gcccttgtat tcttagtatt ccttcaatac ttataatcgt
     1141 ttcttcattt cccgcagtat ttaatactgg aaagcccaaa taaactattt ttccagattg
     1201 taatactatc tcaattttcc ctctcgattt gcgtgtaatc cgtactttac gatgcaccaa
     1261 aataggtaat ctccacgcat ctgaaatatg aagaaattta taattgaagt atatcgtttt
     1321 aggtaaattt aatatattct ttattatcaa tccgagtctc atctttcacc atctcttttt
     1381 gtaatcaaaa ttagtttata aaggattaca cccaatatac cgcccaacgt attatataca
     1441 aaatccgaaa tttgaaacgt tcctagcctc aaaaaaagtt gtaaaaattc aattattgct
     1501 gaaaaataaa atgcaattt
//
LOCUS       DAFGFZ010000252         1493 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000252 DAFGFZ010000000
VERSION     DAFGFZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.259"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            132..506
                     /locus_tag="LR726_003053"
     CDS             132..506
                     /locus_tag="LR726_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL6218059.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="LR726_003054"
     CDS             complement(620..1249)
                     /locus_tag="LR726_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL6218060.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="LR726_003055"
     tRNA            complement(1383..1455)
                     /locus_tag="LR726_003055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          507 a          226 c          261 g          499 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taa
//
LOCUS       DAFGFZ010000253         1455 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000253 DAFGFZ010000000
VERSION     DAFGFZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.260"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..485
                     /locus_tag="LR726_003056"
     CDS             <1..485
                     /locus_tag="LR726_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBL6218061.1"
                     /translation="ISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLE
                     IFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGG
                     GAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN
                     "
     gene            complement(771..>1455)
                     /locus_tag="LR726_003057"
     CDS             complement(771..>1455)
                     /locus_tag="LR726_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6218062.1"
                     /translation="LYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLN
                     QCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMR
                     WTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTS
                     LEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFL
                     WLFRMGVIP"
BASE COUNT          462 a          246 c          266 g          481 t
ORIGIN      
        1 caatttcaaa taaattattt tttggctcat taatatttgc aggagttgta gtttcattat
       61 tattattctt atggatgaat gctagaaaga aagaaatagc agtattacta tcattaggta
      121 tatcaaaatt agaaattttt ggtcaattcc taattgagat ggtatttata tcaatccccg
      181 cattcgttgg ttcctatttc ttagctcaat atacagctga caagcttggg aataatatat
      241 tgaataaggt tactggtgat atagctaaac aaatagctaa acaatctgca tctagccaat
      301 taggtggtgg ggcagaagct gaaggattta acaagacatt atcaagttta gatatcaatg
      361 tattacctaa attcatgatt tatgtagtta tatttatgag tttagtactt cttgtatcat
      421 tgattatttc ttcattcaat atattaagaa ggaatccaaa agaattatta attgacaata
      481 attaaaattt tcggtgcttt ttagcaccaa aaatgagagt gtaatataaa ccgtgtaagt
      541 aaccatcaat cctaggcggg tggttgcttc acggttttta tttttctaaa ataagatcaa
      601 gaggtgatta cacgatggct agaaagaaaa gaaatcctga tgctgagaag ttagctgaat
      661 ccattttgaa tacttatcaa cccgaatctg tagaggatat gcaagatgtt ttaaaagatg
      721 tgtttggtcc cttatttgaa aaaatgcttc aaggagaatt gaataaccat ttagggtatg
      781 acgcccattc taaagagcca aagaaacatg acaatcgacg aaacggttat ggcaataaga
      841 cgcttaaaac gagctttggt aaagtaaaca ttgatgttcc cagagaccgg gaggcttctt
      901 ttgaacctga gttgattcct aatagaaaac gagatgtctc cgacatcgca gactgtagac
      961 aaaagctgtt ccaaactcgt aactcgagct tggaacagct ttttatctac ttcgtttcga
     1021 atttttgaac aaaaaaataa aggctccttg cctttccata gccaggtggc taatttcttt
     1081 aaattcattg cagcaaacac tagcatcgta tgcgtcgcga ctttttccaa gcctcgatac
     1141 ttggtccaac gcatcccatg cttttctttt gcatccgcaa atattcgttc aatcgtttgt
     1201 ttccgttttt tgtaaattga tttattcaga tctgttaggc gaagatgttc cacttcatcc
     1261 atcagatctc cccaaacatg tctcgtgatg actcgttgat gatttttact tatggtacat
     1321 tggtttagca gaggacagtt cacacattct tttggattgg atttgtattc tcggtaccct
     1381 tctcgagtaa ccgttgaaaa agcgagcatt tgatcttgcg gacaaatata acaatcaaaa
     1441 tactcgtcat agaga
//
LOCUS       DAFGFZ010000254         1445 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000254 DAFGFZ010000000
VERSION     DAFGFZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.261"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(468..1082)
                     /locus_tag="LR726_003058"
     CDS             complement(468..1082)
                     /locus_tag="LR726_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL6218063.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          462 a          277 c          237 g          469 t
ORIGIN      
        1 tataaatatc cattatctat ccttcaattt ttgtgtattt tatttctatt agaaaaaggt
       61 atcctgttgg acaattttgt tatatcaggt aaacttcatt gcaataatga taacaaatgt
      121 actataatta ctttgtaaag agttttgggg aagactcttt catcaacgtg tattaagaga
      181 caagaaagaa tccatatgga ttctttcttg tctctttttc cactactttt tatttttctg
      241 acactacata ttcttataac tcatttctca taactgtgaa acaacttata aaaataacaa
      301 actttaaaca aaacaagctt aaaatcaaag atgatccatc ttaaaataac taaaacaaaa
      361 aaagtagtcg ttgttaatcc tcattaataa tcgttttctt tttttcaaac gctccatttt
      421 tatgtggaac attatttttt taaggaactt tgtcaacatg tggataacta ttttccactt
      481 gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt
      541 gcaagagagg ctgttccgca gatcgctgga tctagatttg gcaaagtcaa gactgcatca
      601 tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg caaaccactg
      661 ctatgttgcg atttgatact tcgtgacaaa tcaaatccag taacaatgat taatggatga
      721 ccattttctt tgatccactg agctagttcg atgattgccg ggtttcttcc ttcattcgaa
      781 agaagaagaa aaatgtcttc tgatttgaca gaaagctgat caataattgc ctgtcctgca
      841 ccttctagcg tttcatagac acctccggct ggattttcaa tcaatatact tggtacaaga
      901 tccggggttt gatcccaaaa ttcaaatgct acaccactta gtgtgcggct agcatatatc
      961 tgcaatctac caccttgatt aatttgtttc gcgatccaag tgccagcttg aagcatcagc
     1021 ggctcttcta tccttaattt ctcttccaat tggtgataat agtcaaaata acataaactc
     1081 atttatctct cccgcctagc tatacttgac atgaatagaa acagctgcct aatctaagga
     1141 agtacataaa aaaacagtag ttagacagta acacatgtgg tctttgctgt ttcaaacaca
     1201 aaaaaggcat tgaacgaata cgcccaatac cttgacatag caaataaaac aacgtgcaga
     1261 ccatggaaaa tatctacata ggggtagaat tttcacccta gattaggtac aataaattag
     1321 gtcgatgcac tgcattgact atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt
     1381 cattccctga ttcttagcgc caactaaggg tcagggagcc tttttttatt cagctgtttc
     1441 tatct
//
LOCUS       DAFGFZ010000255         1441 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000255 DAFGFZ010000000
VERSION     DAFGFZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.262"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..290
                     /locus_tag="LR726_003059"
     CDS             <1..290
                     /locus_tag="LR726_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBL6218064.1"
                     /translation="ECNSSQQIHFTSENPVLLLERLKQEAGKDIWICGGANLVQQLVS
                     KNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLCKTQTYNGITDLIYMRR"
     gene            836..1078
                     /locus_tag="LR726_003060"
     CDS             836..1078
                     /locus_tag="LR726_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL6218065.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            1075..>1441
                     /locus_tag="LR726_003061"
     CDS             1075..>1441
                     /locus_tag="LR726_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL6218066.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
BASE COUNT          522 a          196 c          293 g          430 t
ORIGIN      
        1 aagagtgcaa ttcttcccaa caaattcatt ttacaagtga aaatcctgtt ttattattgg
       61 aacggctaaa acaagaagca ggaaaggata tttggatttg tggcggagca aatcttgttc
      121 agcagttggt aagcaaaaat atgattgata aatattacat ttctgttatt cctactctgt
      181 taggaaatgg ggttcgtctt ttgggcaaca tagacaggga aataaagtta aggctttgta
      241 aaacacagac ttataacgga ataaccgatt tgatttatat gcgtagataa ctagagagaa
      301 acagaaaagc tgttaaaccg acggagcgat ccgaccacgt tggcgggaaa atccttaaga
      361 tttttccgcc atgcttgcaa gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc
      421 gttcaaagcc cccgttatct aacaaggggg gcacaaaaaa caagaaacac tatacactaa
      481 caagcggaaa acgctgtatt tataaggcaa aaccccattt ttacagtagt gatataaatt
      541 gtacctatat cactatgaaa attgaatagt aaatatgaga aagacagttg atttcaaaaa
      601 ataagaaatt gattgtcgtt ttcttttgca gaaatttaag tgcaggcaat tatggtagtt
      661 gtcgtaattg ctgtcagctc agtgtttatc actgatggtg tgaaagattt acggttggag
      721 atgttcaata agattattgg tgcttaatta tttttattgt agtgctgata tgagaatgtt
      781 ataattgggg tataaaacat acaaataaat ttgctgaaaa atgaggtatc aaaatatgga
      841 aaatgaatta ctggaaaact taggaagact tcacacaact gaacttggcg ttattagaat
      901 taaaaagaac ttatctttga atgtagaaaa tgtaattgaa tggtgcaaag aaaaaattag
      961 cctttctaat gcggaaatca caagaaaagg aaaaaactgg tatataacga ttgataattg
     1021 tattataacg gtaaatgcgt acagttatac cgttatcact gctcacaagg taaaatgata
     1081 aatttttcta taaaggaatt aaacgcattt gcagttattg ggcaggaagt agaactgact
     1141 aattatcaaa aaaagaatat tcagattagt acacagtttt ggagaaagtt taacagtagt
     1201 ttgaaaaaag cgtatctttc acaatcagga aattgggtaa aatatgcttt tatggaaaga
     1261 agaaatggaa aactttatta tttctgttct attccccaaa gaaccattac cccagagggc
     1321 tttctgtata aagaaatacc gtcttataaa tatttggttg tggagcatat tggtgctatg
     1381 gataaaatat atgaaactta tggcaagatt tatgaagaaa ttattcctag tacatcgtat
     1441 a
//
LOCUS       DAFGFZ010000256         1441 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000256 DAFGFZ010000000
VERSION     DAFGFZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.263"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..402
                     /locus_tag="LR726_003062"
     CDS             <1..402
                     /locus_tag="LR726_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL6218067.1"
                     /translation="REVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENEV
                     TIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDS
                     EEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            407..1354
                     /locus_tag="LR726_003063"
     CDS             407..1354
                     /locus_tag="LR726_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL6218068.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          485 a          204 c          290 g          462 t
ORIGIN      
        1 cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac
       61 gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt aaaaagaatt
      121 gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca gacacctgaa
      181 gtttttgaaa tgaatgcctc aatgtattct tttaaacctg aatttctaaa aaaaagtaat
      241 tatatttttg atgggcaatg tgggataata aaaatgagag attatctaat tttagatatt
      301 gatagtgaag aagattttaa atggttagaa tttttgtttc ctaaattttt agaagattct
      361 tcggagcttc aagatatcta taattattta tatcggaggt aaaaggatga gctgttatct
      421 tatcaccggt ggggctggct tcattggttc taccttagcc aatcatttag gagaagaaca
      481 tgtggtaatc gtcgtcgatg acttatcaat gggaaaagta gaaaatcttg atatgacgaa
      541 aaatatcact tttattgagg gagatgtcgc tgagaaatcc ttgatggaag agatcatgaa
      601 agctaataaa tttgattata tctttcattt agcagcagtt gctagtgtgg cagattcagt
      661 tgcccgacca gtagaaacac atcgtgtgaa ttttgaaagt gtattaatga ttttagaatt
      721 gatccgcaag tatcaacctg atttaaaacg gatcgtgttt agttcttcag cggctgttta
      781 tggggatgaa ccgactttac caaaaaaaga agagtccgtg attcgcccat tgacgcctta
      841 tgcggttgat aaattcgctg cagaacaata tgtgctagat tattgtcatt tatatgatgt
      901 acccggtagc gcagttcgtt ttttcaatgt ctatggtcca aatcaaaatc cgaattctcc
      961 atacagtggt gtgatttcta ttctcgtaga tcgctataag aaacaaatcg taggagaagc
     1021 aacttcattt acattatttg gtgatggaag ccagtctaga gactttgttt atatagatga
     1081 tgtaatccaa gcattacttt tagttgcgaa tgaggaagct gcactgggga aacaattcaa
     1141 tgttggaacg ggtaaagcaa ccacactttt ggaattgatt catgcaatca atgaaattct
     1201 cggtgtagaa ttgacacttg aatacaaaga agagcgttcg ggtgatatcc atgactctct
     1261 agcagatatt actaagatcc agtcaattgg ctatcaaccg aaatatggta ttttaagtgg
     1321 aatgactaat tatttgaaaa ctgaaatcaa ttaaaacata ctaagataag attagagggt
     1381 aaattcaggc tctttgtcaa taaggactga tgagttttgt aaaatcaaat ctgggataga
     1441 a
//
LOCUS       DAFGFZ010000257         1426 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000257 DAFGFZ010000000
VERSION     DAFGFZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.264"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..539
                     /locus_tag="LR726_003064"
     CDS             <1..539
                     /locus_tag="LR726_003064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218069.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            566..>1426
                     /locus_tag="LR726_003065"
     CDS             566..>1426
                     /locus_tag="LR726_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218070.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKK"
BASE COUNT          590 a          224 c          319 g          293 t
ORIGIN      
        1 cggtcatggc agaagaaaaa gaaccactag acaatagtcg tttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga aaagatgttt gaacaccaga
      121 gaaagaccaa cggtcaacca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagagcgaat cgaaaataaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aagtagaaaa acttgaaaga caagaagaga taaaggaaat gggctataac aagtacggcg
      301 gactggatat gacgatcgag aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcggaaaaag gaaaagaaaa ccttcttcct gaagtacaag
      481 caatcattga cagcggacga gtgacgcaat ggaaaaaaat ccgaccattt attttctgaa
      541 aggctatcga aaagtcgctt tagaactgaa tgaaaacggg gaatttgagg aatctaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgt
      661 accaactgat tttttgaagg agaaagaaaa cgatcatttt tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcatactc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaatagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaa
//
LOCUS       DAFGFZ010000258         1424 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000258 DAFGFZ010000000
VERSION     DAFGFZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.265"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(465..686)
                     /locus_tag="LR726_003066"
     CDS             complement(465..686)
                     /locus_tag="LR726_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL6218071.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(864..>1424)
                     /locus_tag="LR726_003067"
     CDS             complement(864..>1424)
                     /locus_tag="LR726_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HBL6218072.1"
                     /translation="ITLFQPFMIIWVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKD
                     AAGIWYADRFRPLITAMINIVLNIILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQN
                     IFKFESAGKYVWKLISLCVIAFVASYLSYFACSYVNIGNFKVISLVINLVISVVISIL
                     VILLFNIRNKKFKYILKVLTSVLVKR"
BASE COUNT          502 a          288 c          137 g          497 t
ORIGIN      
        1 tcgtcttgat aacgagtaag tatttttata cgattgataa catagtaaca atatttttaa
       61 ttatagtaaa aaatcatctg cctttttcat ccacctaatc ttatttttca ttttattcaa
      121 catccttaaa taaacttccg ttcctaagct ctataatatc ttcttaaaaa ataatattat
      181 ggtgtcgttt tcaaactcgt aatcttttac attggaaaaa tacatacttt tctcaatgta
      241 gcaactccta tctcacagta ttcaaaaatt cttcaatatt atgaaatact tgtgcatgtt
      301 ctttaaatac cttgtcagtc tgtttataat cccctactaa attttgcatt gctcccaact
      361 cttcgacaaa ttgtcatctc catctgtaaa cagccctaaa aactcatata atgctttttc
      421 atcaatattt ttcccatatt attctcggta tagtttcaca agttttacgg attcttcata
      481 ttgtttatct ttttataata ttcatctagg gacataatta cttttgctag atttcctgca
      541 acaacacaat cgtctgatac gtccttgttt actaaactat ttgcacctat aactacatta
      601 tttcctatat gtacaccctt tagtattcta gacttcattc ctataaaaac attattacct
      661 attttgatcc tctccgatga tcccaaagct tctccatact acttttaaaa ccgatcaatc
      721 ataaacatga gtcaaaattg taactccttc atatcatctc caatttcaac aagagtcttc
      781 gttggaacaa aaactataca tcgttcgcct attcggcagc ctaaaaattg tagttacttt
      841 atatatcatt ctgatgtctc ttctcatctt tttaccaata ccgacgtaag aactttcaaa
      901 atatacttaa acttcttatt acggatatta aacagaagaa taactaaaat cgatatgaca
      961 accgatataa ctaaatttat cactaaactt atcactttga aatttccgat attaacatac
     1021 gaacaagcaa aataacttaa ataagaagca acaaaagcta ttacacacaa acttatgagc
     1081 ttccaaacat atttacctgc cgattcaaat ttaaatatat tttggaaaac attatataat
     1141 aaccagggta aatcaaccaa caatactgaa acaatggttg ccgccaaaac tccatataat
     1201 ccgaaccaat taacgaggat gatatttaat acaatgttta tcattgctgt aattaatggt
     1261 ctaaacctgt ctgcgtacca tattccagca gcatctttgt ataacccaat taactgatca
     1321 atttcatata agaaaaaatg aactatcata aatataacaa atgaaatact cataagatta
     1381 tcacttccca cccagataat catgaacggc tgaaataatg taat
//
LOCUS       DAFGFZ010000259         1423 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000259 DAFGFZ010000000
VERSION     DAFGFZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.266"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            51..758
                     /locus_tag="LR726_003068"
     CDS             51..758
                     /locus_tag="LR726_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218073.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            1139..1318
                     /locus_tag="LR726_003069"
     CDS             1139..1318
                     /locus_tag="LR726_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218074.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT          515 a          220 c          299 g          389 t
ORIGIN      
        1 tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata atgaataagg
       61 tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaagcaaa gaacacttga
      121 atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta
      181 ttcaaagagt ggaagaacag ttgtatatgg aaagcgaaac aagtcaaacg aaggtcacag
      241 tcagccataa tcatttagac gtgtataaac gtcgtcctga aacggtaaaa aaaggaacag
      301 agcttctacg actagaaaca gaaaaataca tcatgaatgt gtacctagaa aggagtgagc
      361 tggtcgaaaa ctttgtcagc tacacgattt gtaggaaaca ctattactca gaatcagaat
      421 attattcaat tttcgagagc atggaattaa tggaagaaga aatggaagag ctaaacagca
      481 aaaatgcgac ggtagacgat tattaccttg tatttactag gtttttgacg tgtgggacgg
      541 aatctatcgt gttaatggga atggattatg aactcgctgt acccgaagca tttttgaggg
      601 aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta actgattttt
      661 tagacaccta cacttacgac gatacagaag aaatccaaaa tgaacttgac cgtaaacgaa
      721 tgccatatca acgatttgaa aggaaaaaag gtaaataacc atttataccg aaaagtgagg
      781 atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa
      841 tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgggcaaag cccgataacc
      901 taagagaaag aaggagatcc atatgaataa aatcattcct ctattgttcg tactgtttgt
      961 tggcgagata cttatgcgtc atgctgcagc tgtacattta aacttcgtag tattcttagg
     1021 aatactggta ttttgtttga tcttttggcg atttgtatta gcccttgtga gtctactatt
     1081 caagatgatt gcagccatat ttttcatagg gttatttcga tgaaaggaga gagtactaat
     1141 gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga gacaaaaaga
     1201 attgcagcaa ctaaagcaga agtacggaaa aagtgaatct aagattatgt gtattgcgtt
     1261 agatctattg atcgcgcaag aaaaagcagg atttgatata ccagcactaa aaaagtgacg
     1321 tcacttttca tttgaagaaa caaaagtgat agcacttaat tgttcctact atttagaata
     1381 ttttctatgt cctttttatt ccattttgta tcaataacta tga
//
LOCUS       DAFGFZ010000260         1416 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000260 DAFGFZ010000000
VERSION     DAFGFZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.267"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            31..441
                     /gene="spx"
                     /locus_tag="LR726_003070"
     CDS             31..441
                     /gene="spx"
                     /locus_tag="LR726_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL6218075.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            455..811
                     /locus_tag="LR726_003071"
     CDS             455..811
                     /locus_tag="LR726_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL6218076.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          521 a          209 c          265 g          421 t
ORIGIN      
        1 attggattat aattaaaaag gagtgagaaa atgataaaag tatatacttc gcctagctgt
       61 acttcctgta gaaaagcaag agcatggttg caagcgaatc aacttgaatt tgaagagaag
      121 aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc agcgactgaa
      181 ggaggagttc aagatattat ttccactcgg tcgaaaattt acgaaaagct gggcatagat
      241 tttaacgaat taactttaaa gcaaatgatc gtcttattca aagaatatcc ttcattattg
      301 agacgaccta ttttgattga tgaaaaaaga atactaatcg gatttaatga agatgagatc
      361 cggtcatttg tccctcgtga aattcgatgt gttgccagaa atctgatcta tttacaaaac
      421 aataacatct taaattcata gggagggagc cttcgtggaa gtgaaagtct atggatcagc
      481 gcactgtgta acaagtctga aggcgaaaca atggttagaa caccatcaat tgccttatca
      541 atttgttgac ttagaagaac gcgatctaag tacagaagaa gcagaagaat tatgcagttt
      601 tgaagaagtg caagcagaac agctatttgc gacttggtct gaggcttttc acaacattgc
      661 gattgacctt tcctgtgtag gaaaaaaaca actgattttt ttatgcacac aacataccaa
      721 tctcctcaga cgtcctttga ttattatcga tgaagttcta tttattggat acaatgagca
      781 gttgatggaa cagcttatct taaataaata gactttaact gttttaatca agtcactgag
      841 catttcatac agttgtaaca gtcgaacact tagcatcaag cgtgttatgt gttctttttt
      901 tgactttatt tttctactat agagacatgc tcaatttatg tggtcaaaat tcaaatctag
      961 gtggggatat agagttaaaa atatattgac atgtgtaaat ttacatgata tattcatttc
     1021 tgttgtttta agtagcaaag caataaaata gtattttaaa aaacatataa aatagttgtt
     1081 gacatgttag aaatgacatg ataagataac tgagttgtta aaaaagaacc acttgtgaag
     1141 cttgattcta tcgaaaaaaa cttcaaaaaa gttcttgaca aataacaaat gataagttat
     1201 gataaatgag ttgctgtaag atttcgaaaa acgaaatcaa aaaaactttt aaaaaagagt
     1261 tgacaattac ttgtctctct gatatgatat aaaagttgct acggcagcca aaaacgaatc
     1321 ggggaacact cctgatgaag tagacctttg aaaactgaac aaagtaagac aaaccaaatg
     1381 tgtagggcgt cttgattcaa ttcaagacaa caaaca
//
LOCUS       DAFGFZ010000261         1395 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000261 DAFGFZ010000000
VERSION     DAFGFZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.268"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            134..1303
                     /locus_tag="LR726_003072"
     CDS             134..1303
                     /locus_tag="LR726_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL6218077.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          514 a          207 c          225 g          449 t
ORIGIN      
        1 aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta
       61 gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata
      121 ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg
      181 tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc
      241 tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa
      301 ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt
      361 aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac
      421 caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga
      481 aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa
      541 tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag
      601 atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa
      661 agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt
      721 tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga
      781 ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag
      841 tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa
      901 tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt
      961 ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga tagataaatg
     1021 gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat taacctttta
     1081 taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg aaggttttaa
     1141 caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag aatctctgga
     1201 gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc gagttcataa
     1261 aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc aacagaatgg
     1321 gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct tgcgtaaagt
     1381 tataaaatat taata
//
LOCUS       DAFGFZ010000262         1389 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000262 DAFGFZ010000000
VERSION     DAFGFZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.269"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            311..565
                     /locus_tag="LR726_003073"
     CDS             311..565
                     /locus_tag="LR726_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL6218078.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            566..883
                     /locus_tag="LR726_003074"
     CDS             566..883
                     /locus_tag="LR726_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL6218079.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          481 a          221 c          272 g          415 t
ORIGIN      
        1 ataaaaaggc aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc
       61 gaacggcatt catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg
      121 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
      181 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      241 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      301 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      361 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      421 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      481 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      541 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      601 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      661 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      721 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      781 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      841 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      901 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      961 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
     1021 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
     1081 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1141 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1201 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1261 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1321 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1381 tgggataaa
//
LOCUS       DAFGFZ010000263         1386 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000263 DAFGFZ010000000
VERSION     DAFGFZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.270"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            34..978
                     /locus_tag="LR726_003075"
     CDS             34..978
                     /locus_tag="LR726_003075"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL6218080.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          495 a          206 c          277 g          408 t
ORIGIN      
        1 taacaaatag ttatgaaaag gagctgttta ttcatgaaaa aaacaagtcg taaagtagta
       61 atcgtcggta caggttttgt tggaacaagt atcgcttatg caatgatcaa ccaaggaatc
      121 tctaatgaat tagtattgat cgatgtgaat caagaaaaag cagaagggga agcattggac
      181 cttttagatg gtatggcttg gggcgatgaa aatgtggctg tatggtcagg tggctatgaa
      241 gaatgtaaag atgctgatat cgttgttatc acagctggta tcaaccaaaa acctggtcaa
      301 tctcgtttag atttagttaa aacaaatgcg tcaatcatgc gccaaatcgt aaaagaaatc
      361 atgggatcag gattcgacgg tattatcgtt gttgcttcaa acccagtaga tattttgaca
      421 tatatcgctt ggaacgaatc tggtctgcca acatcacgtg ttatcggtac tggaacaaca
      481 ttggatacaa ctcgtttccg taaagaaatc gcattgaaat taaaagttga cccacgcagc
      541 gtccacggct atatcttagg tgaacatggt gattcagaag ttgctgcatg gtcacataca
      601 actgtcggtg gtaaaccagt gtttgaaatt gtagaaaaag accatcgtat tgcccaagat
      661 gaattagatg tgattgctga taaagtccgt aatgctgctt atgagatcat tgatcgtaaa
      721 aaagcaactt actatggtat tggtatgagt actgcacgta tcgtaaaagc tatcttgaat
      781 aacgaacaag ctgtattgcc tgtatctgct tatttaactg gtgaatacga cgaaaaagat
      841 atctttacag gtgtgccatc aatcgttgac gaaaatggtg ttcgtgaagt tgttgaatta
      901 tcaatcaacg aagaagaaaa agcaatgttc agcaaatcaa caagtgcttt aagagaagta
      961 ttgaacactg tattataaga aacaatgaat atgaactaaa aatcctaaaa ctgcttaaca
     1021 gctgaaatag ctgttaagca gttttttatt gatatctaat aggtaagcaa ataaacagaa
     1081 tggttaaaat taatcggtcc gaaaatgtat ccgtacatat gaacatatga aatttaatct
     1141 gttgattaca ataagcgaat cgttatcaat gtttatctgt ggttaattaa aaaatccttc
     1201 cgcaacggca aatacaatga ttcatatcta attcgttcat atttgattaa ctgaataaag
     1261 aaactggttt ttatcattca attaaacgac aaaaaaagta agaaaaactg caaaattttt
     1321 ttataatttt gcataagcag attttgatgg aaaaatgacg atttttgata aaaaaccgtt
     1381 cgagag
//
LOCUS       DAFGFZ010000264         1375 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000264 DAFGFZ010000000
VERSION     DAFGFZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.271"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            9..341
                     /locus_tag="LR726_003076"
     CDS             9..341
                     /locus_tag="LR726_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL6218081.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            960..1301
                     /locus_tag="LR726_003077"
     CDS             960..1301
                     /locus_tag="LR726_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL6218082.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          491 a          182 c          306 g          396 t
ORIGIN      
        1 tttattttat gtcatataca ggaattttga gtctcaaaga tatttgtcac tacggtaaac
       61 ggtgtaccgc tacagagaaa atcacgaaga aactatcaac tggccaaagt aaaaccgttg
      121 tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaggaaatg atttattaca
      181 ttgggaaaca aaagcaaata attttaaaag atcctatccc cttaaaagag ctatatccaa
      241 caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa aatggagtct
      301 tgcgttgtac cgcaaaaggg atggggcgtt tgatcggctg aattgatcat tctcataact
      361 tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa ggaacttaga
      421 gtcactagaa aaataaggag tagacttgat ctatcaacaa ttcccttcgg ggagtgacgg
      481 ggtaggtttt aacgattggt aacgtaaaaa attggtcaat cgagtaaatg cgcttgtttg
      541 gatagtggtg aaatgaattt gtgtaaataa taagcgcaga gtaaacaaga gggaatttag
      601 aagttagaca agttagaata gcgtctagat taatcatgag cagctactaa gggatctaga
      661 ctttgacgca aactcatgga ataaggagta ggtcttctcg taatggggag gacgcacaag
      721 aaattgtgtc gtgtgctgca gtacatgtag atacttgcga ggacgttgat tattggatca
      781 atcgagcatt taccgctgtc tactagggtt tctcgtttcg gacgccataa acgagtcgaa
      841 attttttatg tatttttaag taaacgaaaa acgataaaat atgaacaagt cctgaataaa
      901 gtgaaaaaat taatataaag catagagaaa atgaaattta cgtaatggag gaatgacaaa
      961 tgaaaaatta tgatccgaat atacgattgg gcacacatac tattaaggtt tctttccaac
     1021 gttgggatta caaaggattt gtgacgttta gaaggggcgg aaattgtaaa ggattagatg
     1081 ttttagcatt agacgaggat gatttatacg atcaaacatt gactgataat ccaattggat
     1141 tcggcttgct acctgaagat gatgaaggta atgaatggtt taaaatgacg cttatgaatg
     1201 ataatggaga cgaattatct gttgaagata cttggtcata tttgagtgat tatattgtca
     1261 gctttgaaat cattgaattt gtagcggata aagaagaata aaaactggag gaataaattt
     1321 tacagataaa ggcttagaag gagtcacgac gattaataaa aaggtagtat tacct
//
LOCUS       DAFGFZ010000265         1374 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000265 DAFGFZ010000000
VERSION     DAFGFZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.272"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            39..1340
                     /locus_tag="LR726_003078"
     CDS             39..1340
                     /locus_tag="LR726_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL6218083.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DAFGFZ010000266         1362 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000266 DAFGFZ010000000
VERSION     DAFGFZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.273"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            82..>1362
                     /locus_tag="LR726_003079"
     CDS             82..>1362
                     /locus_tag="LR726_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL6218084.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAFGFZ010000267         1361 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000267 DAFGFZ010000000
VERSION     DAFGFZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.274"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(28..>1361)
                     /locus_tag="LR726_003080"
     CDS             complement(28..>1361)
                     /locus_tag="LR726_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218085.1"
                     /translation="QFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNR
                     FNYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTL
                     EGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLT
                     LFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYF
                     FYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYF
                     LVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQIS
                     QYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHAD
                     LIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          440 a          188 c          243 g          490 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc t
//
LOCUS       DAFGFZ010000268         1343 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000268 DAFGFZ010000000
VERSION     DAFGFZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.276"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..517)
                     /locus_tag="LR726_003081"
     CDS             complement(<1..517)
                     /locus_tag="LR726_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL6218086.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFL"
     gene            complement(627..1196)
                     /locus_tag="LR726_003082"
     CDS             complement(627..1196)
                     /locus_tag="LR726_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL6218087.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          492 a          257 c          177 g          417 t
ORIGIN      
        1 acaagaatac accaattact aacatggcga ttagaaccaa tccaatttta aagttaatga
       61 aaaagcctaa ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca
      121 agtgagcaat tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc
      181 ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt
      241 ttctcaaatt agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat
      301 aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac cgcataactc ttagctaaat
      361 attcatctcc tacaataatt agagactgaa gaaatttgta aatactgtaa tatccataga
      421 ctgttaatac gacagaaaaa tcagaaaata aagtggctaa ccatccgtag tatttcattt
      481 tagggacata actaaataat tttttgtata ctttcatttt ttcctccccc gcattcaatt
      541 tcttctctta ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat tagagatgta
      601 caaacagata aaaggtaaac tacgttctat tttttgtatt tttttaagac ttttcttcca
      661 atcaatacac caatcataga taaacttgct acaactaaca ctgaaatgat tccccatata
      721 cctaccgcat agtggatcat tgcatccatt gttgcagcag attgtccttt atctatccaa
      781 tcttgacgaa aacgatctgt aaagaaccat ataggaatcc actgtccggc agcactgcct
      841 aattgcatta gagcccaaga cgcaacttga ggaattgctc gatcatatcc aaatacagat
      901 agaataatat cagctaagat cgccatacta atcgaactga taaaccaagg taagtacgcc
      961 ccgcccatta acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa aattcctttt
     1021 ctagggtagt tatagctaat ataaacaaat atgattccag ataataatcc aaaaatcccg
     1081 ggggatatgc tgtgaccaaa cgccccacca atactactaa ccattattga aaaaaagtaa
     1141 attactaggg aaataactgt agctaaacca ataaaaatat aatcttttgt tttcatatat
     1201 attctcctca attctatttc gttatgtcat taaaaatctg ttctatcaaa gttgaatctt
     1261 ctactctaag ttttatatct ttttttattc caccattttt taaataaata attctattgc
     1321 atgttttata aagaaattcc gga
//
LOCUS       DAFGFZ010000269         1335 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000269 DAFGFZ010000000
VERSION     DAFGFZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.277"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            627..785
                     /locus_tag="LR726_003083"
     CDS             627..785
                     /locus_tag="LR726_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218088.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            857..>1335
                     /locus_tag="LR726_003084"
     CDS             857..>1335
                     /locus_tag="LR726_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6218089.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIA"
BASE COUNT          520 a          185 c          217 g          413 t
ORIGIN      
        1 caaccctaaa ttttatagtt tagatttttt tctgttgctg aagattagaa tgacaaaact
       61 atttgaaact acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa
      121 atgagaacat gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttgaagtgga
      181 tagcgggttg aataagttga taagcatcaa aacatttata aaaattattc aagactatgt
      241 ctgttccata tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc
      301 tttttataca tgtttatcgt tattaatagt gaataggaaa aaaactattt ttgatcattg
      361 acaattaaat acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg
      421 ccatgacctg attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg
      481 ccatgacaat agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg
      541 tgaaattcct atgtgcttac ccaaagcaat tcaaaaattt cataactact attttaggag
      601 atgattttat agaatccaga gtctacctga ataaaaaaca aacctgtgca tttctaggaa
      661 tcgataataa tactctagat aagtggattc gtatatataa tttcccagta ataagagttg
      721 gtggtgtagt tcgctttgac gtagaagata ttaaagactt tatgaataca tttaaaataa
      781 cttgaaattt tcttctcgcc gcatatacta aaattagtga aatatactgc cttacttttt
      841 ttaaggagtg aggattttgc ctacaaagct ttcaaatggg aaatataaaa ctaatctgag
      901 atatccaaaa aggtttaaag aaataacagg aattgcatca gaaaagtatc aaaaaacatt
      961 tccaaatagg caattggcga ttaaagccga aaatgatatg aagaagaaaa tagaaaaagt
     1021 tttgagagag gagaatgcaa actcattaga attaaaagga aaaataacgt ttaaaaaatt
     1081 ttacgaatct aaatggcttc ctagatatga attaggtcaa acaattagat caaacaggcc
     1141 tcctagtgat ataaccatta gtaatacaaa agatatattt cggttgcata ttttacctat
     1201 gtttggtgaa tacgcaatga attatctaaa tatcaatact gagataatat cagatgaatt
     1261 aaccaaaaaa tcgaaagaat atgcaaatat aaaaatcata aaaggatatg ttcgttccat
     1321 gttcgatatt gcaga
//
LOCUS       DAFGFZ010000270         1330 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000270 DAFGFZ010000000
VERSION     DAFGFZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.278"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..190
                     /locus_tag="LR726_003085"
     CDS             <1..190
                     /locus_tag="LR726_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218090.1"
                     /translation="KTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSY
                     KSAGNNWIATYSGTLTRK"
     gene            200..403
                     /locus_tag="LR726_003086"
     CDS             200..403
                     /locus_tag="LR726_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218091.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            614..1198
                     /locus_tag="LR726_003087"
     CDS             614..1198
                     /locus_tag="LR726_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6218092.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          471 a          184 c          254 g          421 t
ORIGIN      
        1 taaaacgata cgagtgacag gaacgatgat ttttgataaa gttggttggc cttctagacc
       61 tcctcaatca cggaattgga aagaaaaaag aaatggttat tggtactatg gaaaattgta
      121 tttacaaagt tataaatcag ctggaaacaa ttggattgcg acttattcag gaaccttaac
      181 tagaaagtag ggatttttta tgccagaatt tgtgaacaca ccaaattttt atcaagatag
      241 cgatattcta agtaaaatta atttaccgaa agaagaatca gctttacaag ttcagcgaac
      301 tttacaggag acacaaaaaa ttcaaaaatc aatttctaat ctagaagatt ctgaagaaaa
      361 aagaaaaaaa agagaccaaa aaacatatca aaaagaaaaa taatgatttt aaaaaaagag
      421 tgtctagtcg acattctttt tttctaattg aaagtgttca taaaatcacc catttctgga
      481 cactttcttt ttttgcttta tttttcaaaa aaacaattca tttcatgttc ataaacccat
      541 aacaatatct atgttcagtt aaactatatg gataaagtta tgttacaata aaattaatga
      601 aggagtgatt tttttgaaaa taggatacgc ccgggtatct actggtttac aaaatttgga
      661 tttacaagaa gacagcctaa ataaatttgg ttgtgaaaaa atttttactg atcatatgag
      721 tggggcaaaa agtaatcgac caggattaga aatggccatt gattttgttc gctctggcga
      781 tacacttgtc gtttggcgct tagatcgttt aggtagaaat atggaagatt tgatttcaat
      841 cgtcaatcga ctgaatgagc gtggtgtgag ttttcatagt attcaagaaa atattacgat
      901 ggacaaatca agttctacag gacagctcat gtttcatttg tttgcggcat ttgcagaatt
      961 tgagcgtaat ttgatcctgg aacgatccgc agcaggacga gcagctgctc gtgcaagagg
     1021 acggtttggt ggtcgtcctg aaaaattgtc tgaacaagat ttggaattac tgaagacttt
     1081 agtggatagc ggtacaccta ttaagactat tgcggaacgg tggaacgtct caagaaccac
     1141 catttatcgt tatttggcaa aaatggagga gaaaaatgga aagacaaatt ctcattagtt
     1201 tttgtcattt aatgctgaaa atttttgcca tttatctaga aaagtgacaa tagtatgaga
     1261 attttttgta agtgtctact gataacttat tctcaaatgt tgtaaggatt ttattcattt
     1321 gttaatatta
//
LOCUS       DAFGFZ010000271         1324 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000271 DAFGFZ010000000
VERSION     DAFGFZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.279"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..917
                     /locus_tag="LR726_003088"
     CDS             <1..917
                     /locus_tag="LR726_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218093.1"
                     /translation="QTEQEQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKL
                     LQTKMNTNLSQQSQTFILDIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYV
                     YEIIIRDDVYSSPTGIVIAGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNG
                     SLRSTILPFAQSDVVNTVVQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQN
                     QFIFPLLNMIALLPVEETAIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPI
                     WIKRKHYFDIDNNIGYRLRKERANDYNN"
     gene            901..1071
                     /locus_tag="LR726_003089"
     CDS             901..1071
                     /locus_tag="LR726_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218094.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT          499 a          173 c          210 g          442 t
ORIGIN      
        1 aacagaccga gcaagaacaa atgattgggg tttttcaaaa aataatttca ggtttatttg
       61 agagtataga agtagatatt aactttttaa taagccaagg tacacaaata gacaaagatg
      121 tcctagtgaa gttattacaa acaaaaatga ataccaattt gtctcaacag tcccagacat
      181 tcattttaga tattgaaaag gcgttatttc ttagtaacta tggagaaata ttaaaagata
      241 ttcttaatag tatttctaca atggaaggtg tttcagaagc tatatcagag gaaattcaaa
      301 gttacgttta tgaaattata attagagacg atgtttattc ttcccctaca ggaatagtta
      361 tagcaggata tggaagaatg gatattttcc ctaaactata ttcttataat atgtttggat
      421 ttgttatgaa tattctgaaa tactcagaat atgaaagtgc tcaaatagga aatgacaatg
      481 gttcattaag atctactata ttaccatttg ctcagtctga tgtggtaaat actgtagttc
      541 aaggggtaga tccacaaatt actaattatc tgtcgagtca agtcgatagt tttgacgata
      601 acggaaaaaa tacctatatt aatattataa agaatatttc agagtttcaa caaaatcaat
      661 tcatatttcc gttactaaac atgattgctt tactcccagt tgaagaaaca gcaattatcg
      721 cagagacttt attaaattta accagtttta aacgaaaata tactacttct gtagaaacag
      781 taggtggccc aattgatgtt ttagctatta cacctaatga tggccctatt tggattaaaa
      841 gaaaacatta ttttgatatt gataataata ttggatacag attgcgaaag gagagagcga
      901 atgattacaa taattaaacc tcaggaaaaa tcccctgttt tgaaatttaa tgagagcaat
      961 ctagcccata ataaaatgag tataactgat acagtatttg atgtaatggt tgagcaacaa
     1021 aaaaaaatta ataataacaa tagacaaaga aaagaaatta accaatatta atgttttttc
     1081 tgttgcgttt tttgcagtca taatttaaaa cctcgctcaa tcttgggcga ggtctttttt
     1141 tatttttctg aaaagttaaa gtaaataata gcataaaata atatatttta caaagaataa
     1201 gtacaatcta gttttttgct attaaatgtg taataataaa tatgccatca caacatgaag
     1261 aatgaaatcc attattatct agtctagtcc attctttttg tttgcagtat ttgtgatggc
     1321 tttc
//
LOCUS       DAFGFZ010000272         1322 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000272 DAFGFZ010000000
VERSION     DAFGFZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.280"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            90..563
                     /locus_tag="LR726_003090"
     CDS             90..563
                     /locus_tag="LR726_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL6218095.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            572..799
                     /locus_tag="LR726_003091"
     CDS             572..799
                     /locus_tag="LR726_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218096.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          420 a          202 c          216 g          484 t
ORIGIN      
        1 atatttttat tgtttttcga taaaaaatag ttgttttaca ataattattc tgctatattt
       61 tagctatcaa agataaaaga gggataaaaa tgaaatttaa aacattgcta ttaaaatttg
      121 tcgttttagg tataagtgcc gtatttattt tgttcggcgt acttttttat actcagatcg
      181 ctacaagcga aaaaggattc aattttgatt ggcagaccac attatttgtt tctgtcattt
      241 actttgccct tctcctaggg atcgttattg cttatttctt attgaaacta ttgaaaaaaa
      301 tcgaagataa ccaagctttt tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa
      361 ccattttgtt tattggtttc gccttttttg gcttacttcc taaatttatg aaagtagcgg
      421 atttagacga tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc attccttttg
      481 cagtatacac attaacaagc gtgctggaaa aactgcttga acatgcgatt ttacttaaga
      541 cagatcatga tttgacagtg tgaggtgcga catggcaatt atcgttaatt tagatgtagt
      601 attagctcaa aaaaaaatga gtgtgactga attgagtgag aaagtgggca ttacgatggc
      661 aaacatctct attctaaaaa atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa
      721 aatctgccaa gttttggagt gtcaaccagg tgatttgttg acctataaaa atgactcgaa
      781 tactatacca gaagaataaa ataaagtaga acaaacagta aataaaaaat aatctgaatg
      841 atagatgtgt gagactttta gaagttattt acgtctatta ttcagatttt ttatttcgat
      901 tgaataccgt tttatctata aataagatat gtactgactc atacaaaggg ggccttttct
      961 tctccatgta ctatcacttt tcctgataca actggtttcc tgttacgttg tagttggatc
     1021 tgcttttagt ataatacttt attcattcca ccagactgat agcagtttga gcagtctaaa
     1081 taatcatgta gtattacagg ccattctgtt tcataccttg aagtgcttaa aacttcagct
     1141 aatggcccat tgtgttaact cttactccat tcttttttag taagctccca tatatactgt
     1201 aaagccttct atatattatg tcatgttgct ttttaacatt tactatgtag ttttgataaa
     1261 atgatttgaa taacagtgat ggacaattct tttttatttt taacattatt ttgataataa
     1321 tg
//
LOCUS       DAFGFZ010000273         1317 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000273 DAFGFZ010000000
VERSION     DAFGFZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.281"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..990)
                     /locus_tag="LR726_003092"
     CDS             complement(<1..990)
                     /locus_tag="LR726_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL6218097.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQR"
BASE COUNT          387 a          214 c          187 g          529 t
ORIGIN      
        1 tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata
       61 aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt
      121 cagtaaagtc atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata
      181 aacgacaata gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac
      241 ggtttgttga gcttcactgt aacttgaaga aaatgttttt atcaagttta atacattttc
      301 tggtacaaat gtatttatac tgtcctcttg catgatgtcg acgaagttat ctagcaaaac
      361 ttgattttgt aactggtcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt
      421 agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga
      481 ttttgccgta tttgcgattt tcgcaattac ggtagaatca ttgatttttc gcggttttgc
      541 cgtatttgcg attttcgcaa ttacggtgga atcagtattg tcttcctctt ccatgatttt
      601 aaagacgtct tgattcgtaa cttctggctt gcctaagtac aataaattag gcatagattc
      661 aatcttacca tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc
      721 taattctttt ttgatcttag atactttacc ttcgcggcat tgtaacaatt tcattaattc
      781 ttcaactgtg aatatgaaga atatattatc atcagaatct acccagttat ttttgactga
      841 ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg aaagatccat
      901 gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc
      961 ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt
     1021 tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta
     1081 ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt
     1141 accttaaaaa ataaattaaa gcaaaccgct ctatttcagg gtttaagctc atggtaacta
     1201 tatcacgata ttacaataac attatatcac gatatcgtaa tattacaata tgaagataag
     1261 cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat attatga
//
LOCUS       DAFGFZ010000274         1308 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000274 DAFGFZ010000000
VERSION     DAFGFZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.282"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            146..1021
                     /locus_tag="LR726_003093"
     CDS             146..1021
                     /locus_tag="LR726_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218098.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1166..>1308)
                     /locus_tag="LR726_003094"
     CDS             complement(1166..>1308)
                     /locus_tag="LR726_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL6218099.1"
                     /translation="PESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKIYPATKEICH
                     DL"
BASE COUNT          465 a          241 c          226 g          376 t
ORIGIN      
        1 ctcacattct ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat
       61 tatcaaatat gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat
      121 aagtgtttca tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt
      181 tttggaacgc ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag
      241 caagtacttg tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga
      301 tgaaaagacc aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca
      361 catggctgct gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact
      421 ttccgaactc tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac
      481 agagattctt atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca
      541 tgcaataaat tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa
      601 gaaaaacaaa aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga
      661 agcagttgaa tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga
      721 agtgattgct actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct
      781 gatagaggac tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa
      841 aaggcaaatg ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa
      901 acgctactcc ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa
      961 gaaaaaaata gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata
     1021 gcaagtaaac gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa
     1081 aacacgaatc atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat
     1141 ttgactatta ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt
     1201 agattttccg gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt
     1261 ccactgcatc tgtatattgg ataaaattct gacaacaact ttcaggtg
//
LOCUS       DAFGFZ010000275         1304 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000275 DAFGFZ010000000
VERSION     DAFGFZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.283"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            54..956
                     /locus_tag="LR726_003095"
     CDS             54..956
                     /locus_tag="LR726_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL6218100.1"
                     /translation="MNQSVAKTAHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKS
                     NCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSD
                     LPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVIT
                     LVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNV
                     NDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRK
                     SLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1089..1295
                     /locus_tag="LR726_003096"
     CDS             1089..1295
                     /locus_tag="LR726_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL6218101.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          451 a          208 c          260 g          385 t
ORIGIN      
        1 cctataccca tcttacaacg gatgaacttg taataataga gtcttatttc aaaatgaatc
       61 aatctgttgc taaaactgcc cattgcttga atcgttcaag acaaacgatc cataaagtat
      121 atctgttctt caagcaagga aaatcagcct tagaatatta tcaacagtat aagaaaaaca
      181 aatcaaactg tggtagacgt ccgcttgttt tacccgagga acaatcagaa tatattcaaa
      241 gaaaggttgt tcaaggatgg acacccgatg tgattgttgg tcgtgcagcg tttcctatta
      301 gttgttctgc tcgtaccatt tatcgtatgt tcaaaaaggg gctatttgat tcttctgact
      361 taccgatgaa aggcaagcgt aaaccgaatg gacatcaaga aagacgtgga aaacaaactt
      421 tccgccgctc tattcatgaa cgtgaaaagg attatagcca attctcaaat gagtttggtc
      481 accttgaagg tgacactatc gtaggtctga aacataaaag tgctgtaatt accttagttg
      541 aacgattatc aaaagttatc atcacattga aaccgtgtgg tagacaagcg attgatattg
      601 aaaaaaaatt aaatcaatgg tttgaatctg taccgaaaaa cctattcaaa tccatcactt
      661 ttgattgtgg aaaggaattt tcaaattgga aacagatcag taatgtcaat gatattgcca
      721 tttatttcgc tgatccagga acgccgtctc aaagaggcct aaacgagaat tctaacggat
      781 tgttacgtag agatggttta ttgaaatcta tggatttcaa ttcagtagat gaatttttta
      841 ttcaatctgt cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac
      901 ctttggaagt atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat
      961 taagaaagat aaaattgaac aactggacgg gcaaattgat gccttgcttt cagaaagaga
     1021 aatattgata gaagaaaaca agcgattgaa aaagaaagta gcccaaccaa tgaagggagg
     1081 tggatagtat gaacaataga acctttgata ttttgaaatg gatcgctata gtagttatcc
     1141 ccgctttagc gacttttgtc ggtcttgtcg gaaaaggtgt taactggcgg tacacagata
     1201 taacggtgat gattattaca ggttttggag catttctagg gagtatttta ggtgtatcaa
     1261 atcgaacgta taagaaaatt tcatcagata aataggagga taga
//
LOCUS       DAFGFZ010000276         1296 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000276 DAFGFZ010000000
VERSION     DAFGFZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.284"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            258..626
                     /locus_tag="LR726_003097"
     CDS             258..626
                     /locus_tag="LR726_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218102.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            817..1077
                     /locus_tag="LR726_003098"
     CDS             817..1077
                     /locus_tag="LR726_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218103.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
BASE COUNT          514 a          146 c          240 g          396 t
ORIGIN      
        1 acacttagac cagatcatga acagttgttt atgagtttta aaaaacaatt gaaagaaata
       61 caaaaaagat ataacaaggt gttttctaat gcgtgaaaag ctaaaagaaa tcagagtgtt
      121 ttggataatt aacaaagaat ttattatctt gtatacactt ggatttatca gcatgatctt
      181 gttttggtgg ttatatttaa aaaagtaatt taaaagtgtc aaggagattt ttatccagag
      241 aaggatgtgg aatttgcatg tggaataata aattaaaaac aggcttgtta ttaatagtta
      301 tttcctgcgc tatgatggtt ggcatgataa tacaaaggga acaatcttat tttgaagttt
      361 ctgctaacaa tgtaatagaa agatgccatt atggtcaaca tttttggact ggggaagtca
      421 gagaaaatat agatcgtgag tatgttcaaa gaatcgtttg ggatgcatat tctatcaaag
      481 attatcctaa aagtttaacg agtagattat tttacagcga aaaagataat caaaaactct
      541 cgaatttgat gatgaaaaaa gtaaggaaac ttgcacaaaa ctatttggaa gaaaaagaag
      601 gtaatagtcc aattgacaaa aattgatgac actgcaaaaa aatatagagt tcgaattagc
      661 ttttttcgga gcattgtttt ttaggtgaac tatatttcat cactaaaatc agagataact
      721 aacttgaaac atggtcagat gtgttacttg aagtctctga ataatcaatg ttatctagta
      781 gaattaagca actaaaaaag aaagaagtaa gaaaatatgg catacgaaaa tttacaatta
      841 gtagtcgcag aagttagtgg ggatatatac cttacaaaag taaaagatgg aattatggac
      901 acgaattata ggagagttgc tacagatgat gtgctaagct catcgacaga atggtttcga
      961 aaaaatgaag aatctgctgt tcactttgaa ggaattgatg gaaacattca tagtttgttc
     1021 tatacaaaag acaaagaaaa agcaaaaaaa attcttgata ttttgaaagg aaggtagtta
     1081 attaaccaat gatataagag aatgtgagac caagtaaata cttggtctct ataattttag
     1141 ccactagtca attaaatagc taaacaattt taataataat aaaatatgac tgcttcataa
     1201 ttaagaagtg cagaattttt ctaagaacga tatttccttg ttgaaatgag tattgctaac
     1261 aatttgatca cgaacagcat cgttacaaat gttgta
//
LOCUS       DAFGFZ010000277         1294 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000277 DAFGFZ010000000
VERSION     DAFGFZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.285"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1244
                     /locus_tag="LR726_003099"
     CDS             <1..1244
                     /locus_tag="LR726_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL6218104.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFGFZ010000278         1293 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000278 DAFGFZ010000000
VERSION     DAFGFZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.286"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(99..1229)
                     /locus_tag="LR726_003100"
     CDS             complement(99..1229)
                     /locus_tag="LR726_003100"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL6218105.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          367 a          251 c          209 g          466 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaa
//
LOCUS       DAFGFZ010000279         1280 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000279 DAFGFZ010000000
VERSION     DAFGFZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.287"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            28..1190
                     /locus_tag="LR726_003101"
     CDS             join(28..304,304..1190)
                     /locus_tag="LR726_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL6218106.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          429 a          248 c          280 g          323 t
ORIGIN      
        1 aataaaaaac acactggagg atttttcatg tcaagacgtc aacgaagaac ctattcaaaa
       61 gaattcaaac aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt
      121 cgagagtatg agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg
      181 ggctcattca aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga
      241 aagaaaaata agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc
      301 ggacgaaaag acaagtaatt gatgccaaca agcataaata ttccatatca gcgatgtgca
      361 aaattctaaa tatttctcgt caaacctact attatcaagc gaaaccgatc gaaaatgagt
      421 ccgacttaga agagatcgtt caggaagagt ttattcgaaa ccgaaaggct tacggtaccc
      481 gaaaattgaa gaagtgttta gcaaagcgtg ggcttcaact cagtcggcgc cgaatcggtc
      541 gaatcatgaa acgccgcgga ttgacatcta cctatacgat cgctcatttt aaagggcaac
      601 gaacagcttg taatgaagcg aaaacagcga atgtattaga tcggaccttt acacaagaac
      661 agccattgga agccatcgtt acggatctta cttatgttcg cgtggggaaa aagtggcatt
      721 atatctgctt aatacttgat ttgtttaatc gagaaattat tggttattcc tgtggtgaga
      781 agaaagatgc ctcattggta aaagaagcct ttggacggat accgtattct ttaacagacg
      841 tcaagctttt tcatacagac cggggaaagg aatttgataa ccaaaccatt catgagattc
      901 tgaatggttt tggaattact cgttcattga gtaggaaggg ttgtccgtat gataatgccg
      961 ttgtggaatc aacctataaa tctgtcaaag tagaattcgt gcatcaatac caatttgaga
     1021 cactggcaca gctacgtcta gaattgtttg attatgtgca ttggtggaac tatctacgct
     1081 tacatggcac gttggcgtat gaaacaccga tccaaattcg acaacagaga ttggcgaagc
     1141 gaatccttga taatgagcgc ggatctgata cctctggaga ggcagcgtaa ttgaatgatt
     1201 gtgcttctgc cggagaaaat cagatccgag gatgctcatt gtcaagggca atcgtagcca
     1261 taacaccgca gcattcacaa
//
LOCUS       DAFGFZ010000280         1279 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000280 DAFGFZ010000000
VERSION     DAFGFZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.288"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            208..1011
                     /locus_tag="LR726_003102"
     CDS             208..1011
                     /locus_tag="LR726_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL6218107.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            998..>1279
                     /locus_tag="LR726_003103"
     CDS             998..>1279
                     /locus_tag="LR726_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218108.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEY"
BASE COUNT          513 a          186 c          220 g          360 t
ORIGIN      
        1 tcgcaaaaca tatttcttat gattaataaa aaattttaaa gtaataaaaa gtgattattt
       61 tactttcgaa taacatatga ataatcttta tatccgaacc gatatatatc tttaaaacaa
      121 aataatcaca caatattaca atataatgca tatacgatat tgcaatatca caatattatg
      181 atataattat attataatat aagatgaatg ggggatattt taatggcaag aatattgtca
      241 gtagccaatc aaaaaggtgg agtttctaaa actacgacta gcgttaattt agcagcatgt
      301 ttgaaatttg tttacggtaa aaatgtactt ttagtggaca ttgatcctca gggaaatgct
      361 acagataatg taggtttcga tatagatgga acaaaccaac ctacaattta tgaagtatta
      421 aaaggggagg ctgacattac agatgctatt ctggactata aaggaattga tgttttgcca
      481 gccgacatag ccctttcttc tgcagagagg gaatttactc aagtaggaag tgaacacagg
      541 cttaaaagag tacttcaacc aatcaaggac aactatgatt atattataat tgattgtcca
      601 ccaagtttag gaatgcttac agtcaacgca tttactgttt ctgatgaaat tatcattcca
      661 gttgaagctg cgtatttctc attaaaagga ctaataaagt taagtgaaac tattgaaacg
      721 gtaaaagaat atacgaacga caagcttgaa gtacgaggag tcctatttac taagtataat
      781 aagcgttata acatttctaa agaaatgacg actgcagcaa ccgaaatttc tagagtcatt
      841 ggtgcaaaaa ttttcaaaac ttatattcgt agaacaataa ccgttgacga agcgcaagct
      901 gctggatcag atctaatcaa ttttaataaa ccctcaacag ctgaagatga ctataaagca
      961 tttacagaag aatatttaaa agaagttggt gattttgatg gcagaagata atcgcagtca
     1021 agctgcagta agtagactac taggaaataa aaataaaaag aactacaaac caaaagaagt
     1081 agaagaaatc gaattaacca agaaaaatat aagtaaagtc ataaaagatc aaggaagtag
     1141 tgtaagaaca gcagatagca ttcgtataaa tccaattcta atgtcagcac tcaaatattg
     1201 gacgactatt gctgaatcag acaaaagtaa accagacgtt atagaagatg cgctattaaa
     1261 atccatacct gaagaatac
//
LOCUS       DAFGFZ010000281         1264 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000281 DAFGFZ010000000
VERSION     DAFGFZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.289"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            15..680
                     /locus_tag="LR726_003104"
     CDS             15..680
                     /locus_tag="LR726_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218109.1"
                     /translation="MAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            724..993
                     /locus_tag="LR726_003105"
     CDS             724..993
                     /locus_tag="LR726_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218110.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          461 a          170 c          251 g          382 t
ORIGIN      
        1 tggtggtcta tggggtggcc tattacttgt taagtgagcc aaaacagatc gcggcgtcaa
       61 atattttcac ggcagtaatg acattaatgt gtgtcatgtg cttttctatt tttagttact
      121 tactcgtcca atttatcagg caaaatttaa caatgagtaa acttttcaaa agtttattgg
      181 acttacttat cttaggcagt atgttaacga ctctaacaga tcggttcgaa caatctgaaa
      241 aaacggtaga ggggataaag aacttcttaa cgacgattga tcaatttata aatggatata
      301 tgtatttgtt tgaagtaggc tttattctca ctttgtttgg ttgtgtattt ataaaactca
      361 ttaaaagatc aatcgaaaaa gaaatggtta ttgatctaat tccgacgctg agcggctatg
      421 agtattacgt tgattttgga aagtatgagg agttgatcca tacggatatc gttcacacga
      481 gtatccatga agtaaccgtt aagggcatca gatctacttt aaaagatgga caggagacga
      541 aaacaatcga ccgaagaaaa gaggtctaca atttttataa agttgaagca agtctacgat
      601 atggcattgg gaaattgagg ttagatggga gtaatattca acaaaacaaa ccatatgttt
      661 taggaaaagt ggtgatttag aacttcctat gaaagatgaa taaataagga gaatcttttg
      721 aaaatgattc ttctttacag aattgtgctt atagttggca cggagttaaa aatcttaaag
      781 aaggagcagt tattcaaagt gaaagaacat cataatcagt tactatgtat taaaacgggt
      841 acgatgaaca ttcaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
      901 tcgattcgag agcaacataa gcaagaaaaa ttaatggcta cttatagcca attgattgat
      961 gagtgggaac aattcaagga atctaaaaca taaaattcca tttaaacacc ttatttttta
     1021 aagagcataa ggaaacgtga ccccactttt aaaaaaagtg gttggatagg ctgtgcctat
     1081 cacgtttctg aaaaaaataa atgaccccaa aataccaata tataggatag tacaaggata
     1141 gcaaaggata gtaaaaggat agaggtagga tagcgaaaag gataggatcc ttttcactat
     1201 cctatattat attggaggat atttatgaat gttaaaatta gagatttaga tccaaaattc
     1261 attt
//
LOCUS       DAFGFZ010000282         1235 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000282 DAFGFZ010000000
VERSION     DAFGFZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.290"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            35..532
                     /locus_tag="LR726_003106"
     CDS             35..532
                     /locus_tag="LR726_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL6218111.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1186)
                     /locus_tag="LR726_003107"
     CDS             complement(569..1186)
                     /locus_tag="LR726_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218112.1"
                     /translation="MYSQTIDSLNKRFGNRVLKGIEIGYIASEKDRIIDYLADKDYDL
                     KLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGL
                     SVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFS
                     VGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKRKRIMVPLPV"
BASE COUNT          431 a          208 c          216 g          380 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatcg cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ttttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttctatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtca
//
LOCUS       DAFGFZ010000283         1219 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000283 DAFGFZ010000000
VERSION     DAFGFZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.291"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            216..>1219
                     /locus_tag="LR726_003108"
     CDS             216..>1219
                     /locus_tag="LR726_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218113.1"
                     /translation="MELLEKAYSSTELIDSYEYEDLKYIKDQRNYSAHPVVTYNGNTW
                     ELRDITKETCQDVIRKSYEIIFLKNPILGKKVTIEIIQYASRIHAMSVTPEEFHSALK
                     NSYLNKLSEKAKENLCKTAFKFVFQLPYGNEEADRDRESTFRLLNALIFDNKEFYLKI
                     LKEEISKYRFTAESREEINKSLGENQQITYTKGVFLLTFIATHPELQHDFSDETIQNL
                     QISINDFKPKQQVLTEIEYYYRLRSLAVLGSNNEELQNHLNSLLDPILQQKMRVSSLP
                     SSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIYNKLNQEQFNK
                     "
BASE COUNT          489 a          176 c          185 g          369 t
ORIGIN      
        1 accctagaat tattccatta ttagaagaag ttcgaagtag tttttatcag ggaaattatc
       61 gttctgcaat agctgtcaat tacagtgcgc ttgttttaga tttaattgat aaattatctg
      121 atttagcaaa tatttacgag gatattactt ctaagaaaat tttagaagat atagctagat
      181 taagaaaaag tgatccaaat tctccaaaat gggaaatgga actactagaa aaagcttata
      241 gttcaactga gctaattgat agctatgaat atgaggattt aaaatatatt aaggatcaaa
      301 gaaattattc agctcatcct gtagtcactt acaacggtaa tacttgggaa ttaagagata
      361 taacaaaaga aacttgtcag gatgttatac ggaagtccta tgaaattata ttcctaaaaa
      421 atccaatatt aggaaaaaaa gtaacgatcg aaattataca atatgcaagt cgcatacatg
      481 ctatgtctgt tacccctgaa gaatttcact ctgcactaaa aaatagctat ctaaataaac
      541 taagcgaaaa agcaaaagaa aacctatgta agacagcctt taagtttgtt tttcaattac
      601 cttatggaaa tgaggaagca gatagagata gggaaagtac tttcagacta cttaatgccc
      661 taatttttga taataaagaa ttctatttaa aaattctaaa agaagaaatt tcaaagtata
      721 ggttcacagc tgaatcaaga gaggaaatta ataaatcact tggtgaaaat caacagatca
      781 catatacaaa aggggtcttt ttacttactt ttatagctac ccatccagaa ttacaacatg
      841 atttttcaga cgagacaatt caaaatttac aaatatcaat aaatgatttt aaaccgaaac
      901 aacaagtact aacagaaata gaatattact accgtttacg ttcacttgca gttctaggaa
      961 gcaacaatga agaattacaa aatcacttaa atagtctact tgatcctata cttcagcaaa
     1021 aaatgagagt ttcttcgcta ccatcttcaa cattagaaac aatatataat caatatttat
     1081 attttggtga aaaagagatg ttcatagatt ttatgatcga acatcttacc gatgctagtt
     1141 cattcaggtt agcagataag gatatggaag tattaccatg gatttacaat aaattaaatc
     1201 aggagcaatt taacaaatt
//
LOCUS       DAFGFZ010000284         1198 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000284 DAFGFZ010000000
VERSION     DAFGFZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.292"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            4..963
                     /locus_tag="LR726_003109"
     CDS             4..963
                     /locus_tag="LR726_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL6218114.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          400 a          228 c          242 g          328 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccgg
//
LOCUS       DAFGFZ010000285         1197 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.293, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000285 DAFGFZ010000000
VERSION     DAFGFZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.293"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..1037
                     /locus_tag="LR726_003110"
     CDS             <1..1037
                     /locus_tag="LR726_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL6218115.1"
                     /translation="SVKGGITSIITQMLNFNWEKKNIEMTFIPSYKGGNAIFKSLYFM
                     YAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWY
                     DSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWN
                     SKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEY
                     NLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDI
                     SSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRI
                     LNLYQKGSKE"
BASE COUNT          444 a          127 c          249 g          377 t
ORIGIN      
        1 gctcggtaaa aggtggaatt acaagtataa ttacacaaat gctcaatttt aattgggaaa
       61 aaaagaatat cgagatgact tttataccga gttataaggg tggaaatgca atatttaaat
      121 ctttatattt tatgtatgca tttgctaaaa ttataggtat ctctttattg agtaaaccta
      181 atattattca tatgcacatg tcgtacagag ggagttttag taggaaatat gtgctgaata
      241 agtggtttag attattaaat attcctgtaa tcatccatct tcatggatcg gaatttaaaa
      301 aatggtatga tagtgtttct gtaaaaaaac aaaatcagat tcgcttgctg ttaagagagt
      361 ctgattccac gattgtactt ggagaaaaat ggaagaagat aattaaagaa attgagccaa
      421 attgtaaaac tgttgttgta gaaaattcta ttgtagttcc agaatatacc ataaagtgga
      481 attcaaaaat gtgccgattt ctgtttatgg gagttttgat aaagcgaaaa ggtgtacacg
      541 atttggtaga agcagttaaa aacattgcag aacaatatcc agaaaaaaag tttatttttg
      601 agattgctgg agcaggtgat gaagaaatat cgctcaaaga attgatagaa gaatataatt
      661 tgcaatcgta ttttgaattt acaggttggg ttgtgggaga aaaaaaggag gagtgctata
      721 gaagatcaaa tgtgatgatt ttaccgtcct ataacgaggg attgcctata tctattctag
      781 aggcgatttc ttatggaatg cctgtaattg ctacagatgt aggagatatt tcttcagcag
      841 tacgtgatgg agaaaatgga tatttgtttc aacctggtga cgtatcagcg ttgacatctg
      901 caatactaaa aatcaatgat aaagataaat acgtaaaatt ttcacgttgt tcgaaggatt
      961 tggcaataac gaaatttaac gatggaaatt tctttgatag aattttaaat ctgtatcaaa
     1021 aagggagcaa ggagtgatga caaggaaaat aaaaaatgtt tcgattatgt aatgaactta
     1081 ttaagtgaat aaaatagaga gaaaaaatat tgaaatagac gatgtaaatt tttatccgtg
     1141 taagaattca aatgaatagt agtgttttag tgcttgtaat agagcaagag tacaagc
//
LOCUS       DAFGFZ010000286         1197 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000286 DAFGFZ010000000
VERSION     DAFGFZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.294"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            182..1156
                     /gene="bsh"
                     /locus_tag="LR726_003111"
     CDS             182..1156
                     /gene="bsh"
                     /locus_tag="LR726_003111"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL6218116.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          420 a          148 c          207 g          422 t
ORIGIN      
        1 tgttttctaa atttgacatt tatcttcacg tcctttttat ttgataattt aagtatagaa
       61 ctttgatata gtttatacaa ataaaaacgc ttctttgtgt attctgtgct tttcttttgg
      121 aatgaggaaa actttggagt aatatgaacg tggacagaaa aatatttagg aggaataaac
      181 tatgtgtacg tctattactt atgtaacaag tgatcattat tttggaagga attttgatta
      241 tgaaatatct tacaatgaag tagtcactat tactccaaga aattataagt tgaattttcg
      301 aaaggtaaat gatttggata ctcattatgc aatgattggt attgccgctg gtatagctga
      361 ctaccctctt tattacgatg cgacaaatga aaaaggattg agtatggctg gactaaattt
      421 ttctgggtat gctgattata aagaaataca agaagggaaa gacaatgtat ctccttttga
      481 atttattcct tggattttag gacaatgctc aacagtagga gaagctaaaa aattgttaaa
      541 aaatatcaat ttagcaaata taaattatag tgacgaactt cctttatccc ctttacattg
      601 gctattagct gataaagaaa aatcaattgt cattgaaagt atgaaagatg gacttcatat
      661 atatgataac cctgtgggcg ttcttaccaa taatccttca tttgactatc aattatttaa
      721 tttaaacaat tatcgtgtct tatcgagtga aactcctaaa aataattttt caaatcaaat
      781 aagtttgaat gcctatagcc gcggtatggg agggataggc ttgcctggag atttatcctc
      841 agtatctcgt tttgttaaag cgacttttac gaagctgaat tctgtatctg gagattcaga
      901 gtcagaaagt attagtcaat ttttccatat cttaggttca gtagaacaac aaaaaggttt
      961 gtgtgatgtt ggtgatggaa aatatgaata tacaatttat tcttcttgtt gcaatgttga
     1021 caaaggaatc tattattatc gaacatatga agacagtcaa attactgcaa ttgatatgaa
     1081 taaagaagac ttagatagtc ataagttaat tagttatcca attatagaaa aacaacaaat
     1141 taaatatata aattagttaa tgtgttgtga ttgattattt aatatagata taataca
//
LOCUS       DAFGFZ010000287         1173 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000287 DAFGFZ010000000
VERSION     DAFGFZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.295"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..757
                     /locus_tag="LR726_003112"
     CDS             <1..757
                     /locus_tag="LR726_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL6218117.1"
                     /translation="IIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEE
                     KEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELY
                     ANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPM
                     DRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDE
                     IEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            829..>1173
                     /locus_tag="LR726_003113"
     CDS             829..>1173
                     /locus_tag="LR726_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218118.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          517 a          134 c          192 g          330 t
ORIGIN      
        1 aatcataaaa atatttgata aaattaataa tttattcaag aatgacaatg ctgaattaag
       61 gaagggtctt ttagagttta aacttcaaga actagaactt cattactctt atttagtgaa
      121 aattaccgaa gaaaaagaac aacaaaaagc aataaaagaa cagatgatag aagaggaaaa
      181 agttcgtaga gaaattgatc gtgaaaagaa acgtattgat aaagaacaac gtcaattcaa
      241 ttctgaaatt tccaaattaa tcgcatatat gcaaaaagct aatgcagatg tggaaaaaga
      301 attatatgca aataaaattc aagaactgga agaaaaatta aaagaattag aagttatacg
      361 tgaaaatgta ttacaaagag aattaaatac tagagccggt tatgtgtatg taattagtaa
      421 tattgggtct tttggtgaag acatatataa aattggtatg actagaagat tagaacctat
      481 ggatagaata aaagaattga gtagtgcatc ggttccattt gaatttgacg ttcatgcgat
      541 gattttttca gaggacgctc cgtctttgga aactaaattg cataatcatt ttagaaaaca
      601 agaagttaat aaaattaatc aaagaaaaga atttttcaaa gtttcactag acgaaataga
      661 gaaagttgtt ttagaaaatt ataatggaac agtaacattt acaaaattag ctaaagctga
      721 acaatataga cgatctttag aattgtcaaa agattaaata tataaaaagc cttcgggctt
      781 tttcttttaa caattaatag gaacgtatat tcgaaaggaa tagaaataat gtggttagaa
      841 aaaataaatg acaaaaaatt taagtacacc gaacgttata cggatccctt aactgaaaaa
      901 aagagaaaag tgtccgttac tttatctagc aattcacgcc aagcttggaa tcaagctaac
      961 ttacttttga atgaaaaaat cgcagaaaaa ataaaacgaa atgaagaatt accactaact
     1021 tttggagaat taaaaactaa atgggatgaa aaatacaagc caactgtaaa agaaagttcc
     1081 tatcgaacaa ctcaggttta tctatcatta atttcaaaat atataaaaga tgatgtactt
     1141 gtaaaaaacg ttaatagtaa tcttatacaa gat
//
LOCUS       DAFGFZ010000288         1162 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000288 DAFGFZ010000000
VERSION     DAFGFZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.296"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..838
                     /locus_tag="LR726_003114"
     CDS             <1..838
                     /locus_tag="LR726_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL6218119.1"
                     /translation="SFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIV
                     MNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNK
                     RKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKA
                     KAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADT
                     ISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSR
                     DH"
     gene            871..>1162
                     /locus_tag="LR726_003115"
     CDS             871..>1162
                     /locus_tag="LR726_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL6218120.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGAD"
BASE COUNT          446 a          127 c          215 g          374 t
ORIGIN      
        1 atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc
       61 caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc
      121 tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc
      181 aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt
      241 tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt
      301 taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg
      361 attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat
      421 gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc
      481 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
      541 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
      601 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
      661 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
      721 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
      781 ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag atcattaaaa
      841 atgttcaaga tattttagga gtgattattt ttgaaaatat gttatttgat attggcatac
      901 aatgatattg gcaacttaaa gagattggtc aatcgattga acgaaaatgc agtttttatt
      961 attcatatag ataaaaaaac tgatatagaa ccatttattg aagcatttaa atcatatgat
     1021 aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt attggggtgg tttttccatt
     1081 gtacaagcag aaatcaattt agtaaagaga gcattacaaa atgagaaata tttaaaatat
     1141 gtattattat ctggtgctga tt
//
LOCUS       DAFGFZ010000289         1161 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000289 DAFGFZ010000000
VERSION     DAFGFZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.297"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            52..666
                     /locus_tag="LR726_003116"
     CDS             52..666
                     /locus_tag="LR726_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6218121.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(831..>1161)
                     /locus_tag="LR726_003117"
     CDS             complement(831..>1161)
                     /locus_tag="LR726_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218122.1"
                     /translation="PQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIEL
                     TPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKE
                     PTNTVGD"
BASE COUNT          389 a          204 c          194 g          374 t
ORIGIN      
        1 cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt tttgagagtt
       61 gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga agaaatgaaa
      121 aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat tactcatcga
      181 cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat tgtggaagct
      241 attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta cttgaagaag
      301 aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat tggcaatcct
      361 ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat cgctgaacaa
      421 gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa agcaaacggc
      481 gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca acgtcgcctt
      541 gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa gattgctaaa
      601 gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat ggtcaatgac
      661 aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac atataattac
      721 tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa caaaccattt
      781 tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg ttaatcccct
      841 actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc ttgtgattta
      901 gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat agctagttct
      961 tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt atttggttcc
     1021 gttggagtca attctataga ttcttcataa aaatctctat acataaagct atcattaatt
     1081 ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca ttcttctata
     1141 gttggtttac ttccttgagg a
//
LOCUS       DAFGFZ010000290         1130 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000290 DAFGFZ010000000
VERSION     DAFGFZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.299"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..186
                     /locus_tag="LR726_003118"
     CDS             <1..186
                     /locus_tag="LR726_003118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218123.1"
                     /translation="KWALYITDIPSFGKNVTIFISNIVSGGINDEDNISIVVDSVALN
                     DALVARNPNKIVKYKLN"
     gene            199..789
                     /locus_tag="LR726_003119"
     CDS             199..789
                     /locus_tag="LR726_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL6218124.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          405 a          127 c          230 g          368 t
ORIGIN      
        1 aaatgggcat tatacatcac ggacatccct tcttttggaa aaaatgttac gatttttata
       61 agtaatattg tatccggtgg aatcaacgat gaagataaca tatccatcgt tgtagattca
      121 gtagcattga atgatgctct tgttgcaaga aatccgaata aaattgttaa gtataaattg
      181 aattaatagg agatatgaat ggaaattagc agtaaagaat atttaaaagc ggttcttcgt
      241 atggaaaaga atctatatat agaatctaaa ggacaacaca caaaattact aaaaataatg
      301 aaatcacatc cggactacaa cgcttggcgg tatactcgtc gaatgagaat tacatcttat
      361 tattattcaa agagaaagaa aaatatcatt tatgcgatta tgtatattgt gtcttgcaga
      421 agaatgaata gtttgggaag aaaatgtggt atagagtgtg gtgaaaatgt atttgatgaa
      481 ggtgtaagat tttaccatac acaaggaatt gttattaatg gtaacgccat aataggaaaa
      541 aattgttact tatatgggaa taactgtatt ggaaatgatg gaatcgatcc taagtgtcct
      601 gtaattggaa ataatgtacg tgtatgcgtg ggtgcaaaga ttattggtaa tgtaaaaata
      661 gctaacaatg tagttgttgc ggcaggagct attgttatca aagattgtct agagaatggt
      721 gcaattcttg caggtgtacc ggcaaaaata atagggtatg ttaatttaac aaaagtattt
      781 ttagaataat ttttagcgaa agaaggtgaa taacgatgcg tatttcaata aaaagatggt
      841 caggagcaga ttggacattg ttcacgttgg cattttttgc aatctccata gttcttgtat
      901 atacttttaa cgccccaaga gcaattttat atttgggaga tataattaat atctttgtgt
      961 tattaaatgc acttagcttt agacgcagaa aaggaatata tgctagcaaa aatgagatgc
     1021 tgatagtttt gtttttacta gtttctattt gtagctcagt tattaattta gaaaatatag
     1081 gattgattat ctgggggata cgaaataaca ctcgtatgtt catagtatat
//
LOCUS       DAFGFZ010000291         1103 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000291 DAFGFZ010000000
VERSION     DAFGFZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.300"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(105..965)
                     /locus_tag="LR726_003120"
     CDS             complement(105..965)
                     /locus_tag="LR726_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218125.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          329 a          212 c          161 g          401 t
ORIGIN      
        1 acgactttat tttattactc tatcttgctg ccatacccgt ctatccctaa tcatctttgg
       61 tgaatttttt caaagttagc ataacaaaaa taagtatttt ttccttagtt aatcaatagt
      121 atcatttttt tcatatgatt taattcatca attaattttt tgtttttatt aattttagca
      181 aaagctagtg catcttgaat caattcgagc acttctgcct tcgacttatt tttagcctgt
      241 gcgttcttcg ctaattgggc agcagctcgt gctaagtaat aagtcaaatg gttatccgaa
      301 caaatatcga ttgcgtaatt tagtagtgca tcacttgttt ccaaatcacc gtcttgcgca
      361 aaaaaaacac cacaatgaaa gacgatattt aacacccgcc agatatcttc tgttgtacga
      421 atcggatacg tataaatttt ttcaaacact cgattgaaat aaacttctgc tttttccaac
      481 tctttttctc tagcatagac cattccaata cctgtatatg ccaataaacg aaacaacgtg
      541 ttggctgttt ctgccatcaa caactgatca aaatggaaca acacatccgt aattggtgca
      601 ttcgtaaaaa tcatttcaaa tccttttaga tagtagtaac gtgcaatcag ctcagactcg
      661 tcaattacct tgggatcaat ggctgctaaa atctgtgtca attgttcata ttcactcgta
      721 atcagtgaaa actctgcttt ttctagctgt tcatcaagtt gtgtattgat cacgttcatc
      781 gaagaaaaaa gttcaactaa cggaagattg attcgattac ataatttgat taaaattttt
      841 aacgaaggga cttgaccatt gttctcaaat ttacttaatg ttgcttgtgt acaaattcct
      901 tcagacactt ctttttgaga taagttggca gcctttcttg ccgtaataaa tttttcaatg
      961 ttcattattt tctctccaat cagacctcac acgaaaatca tttctgtctc gattttagca
     1021 tgttttcttt gggaatgctt taggttctgt tgcaaagttt taaataaaga ataaagtccc
     1081 ttacggtatc tatgatttaa gct
//
LOCUS       DAFGFZ010000292         1091 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000292 DAFGFZ010000000
VERSION     DAFGFZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.301"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            81..>1091
                     /locus_tag="LR726_003121"
     CDS             81..>1091
                     /locus_tag="LR726_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL6218126.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLN"
BASE COUNT          401 a          115 c          226 g          349 t
ORIGIN      
        1 aaatcaacaa ttggacaaaa aaaatttaac aggttacttg cactgaaaaa atttttacac
       61 ttataaagga gaaaagtact ttgaagaaaa tttgtttcat tagtggtgta atttctcgtt
      121 ctgggggaac tgaaagagta gggagtatta ttgccaatag cttgtctgat ttaggttatg
      181 atgtcactat tttaagcttt tggaatagtg gagagcctta ttttgggtta gataagacga
      241 ttaaggttga ttatatgtta aatcctaaaa cggagggaaa attatataga acttatttat
      301 atccggtttg gaaattgcac aagtatatat gcaaaaatga atttgatata gtaattgata
      361 ttgatacttt gttgtctttg tatactacaa gggcaattaa aggaacaaag tgcaaattga
      421 tttcttggga acattttaat tactggacaa tggaaatgct gaaagaaaag aagagattca
      481 aggctaaaaa attgataaaa aaatatgcaa agaggcttgt ggttctcaca gaagaagatc
      541 gtcaaaaaca tattgatgaa tatagtttat cagaagattt tgttataact atgccaaatc
      601 cgtgtatatc taatatcgag tacgaatatc attatgataa aaagagattt cttgcagtag
      661 ggcgattagt ggaagagaaa aattttgctg acatgataag tgcttggaaa attgtagaaa
      721 aaaaagtaga tgattgggat cttatcattg taggaaaagg tgaattagaa aaagatcttc
      781 gccagcagat aaaagattta aatctgcaaa gagtaactat agcagggcat tcagaccatg
      841 tggaggaata ttataaatct gcatcttgtt atgtgcttag ttctgtttat gaaggatttc
      901 ctatggttat attagaagca cagtcttatg gattaccagt tatttcttat gattgtaaaa
      961 ctggtccgag agatttggta catcataatt ttaatgggat gttggttgaa gataagaata
     1021 tagaccaatt agctaagagc atgataatgt ttactaagaa tactgattta gcaatgagaa
     1081 tgtcactgaa c
//
LOCUS       DAFGFZ010000293         1087 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000293 DAFGFZ010000000
VERSION     DAFGFZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.302"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..184)
                     /locus_tag="LR726_003122"
     CDS             complement(<1..184)
                     /locus_tag="LR726_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBL6218127.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVT"
     gene            complement(366..1022)
                     /locus_tag="LR726_003123"
     CDS             complement(366..1022)
                     /locus_tag="LR726_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL6218128.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
BASE COUNT          330 a          219 c          132 g          406 t
ORIGIN      
        1 cagtaacaac ctgatgagaa gtattccacc ccattaagac agtatctata tctttggtaa
       61 attctgtata agtgtcgatg ttttcattgt catttccatg accatttagc caagctactc
      121 caccattacc gtccgcaata tatccgtcaa ggctcatggc aataaataaa actacttttc
      181 tcattttttc tcttttataa atacaaacag gaatttattt ctctttttta ctcataaggg
      241 aacaactgat tgctacaaat aaacccatta aaatccatgg aaatgctgct cttataaaat
      301 cactaaccat actttattcc tccttaaatt ccaatttcct tagttctaca aactgaaatt
      361 tttgcctatg aatgtatctt gttctttata cgcaaacagg cttctgtgtg cataatccaa
      421 tgtcttgaaa acggcatttg aattagtcca caaatatctt tattacacca ataatcaatc
      481 agccaaattg ctttttcatt gtcgtttact acattcaacg attctaagta tccttttctt
      541 tctttcggta tttttctttt caattcatca taagataacc gttcaagcat tttctcagtg
      601 ctttcccata cttcgaaaat atataaatat aattcttcca gattaagctg ttttgaaaag
      661 tctgcaatct gctgtttcat gagttcattt cctgttgtaa tgataggtga attgatacgc
      721 tcttgataat tgcctgcaaa aaatacttgt tcatcttcat ttatcactgt atgcacaact
      781 atatcttcaa tgcggaaaat atgccaaata gaataagcaa tcgtcttact atgataacca
      841 tcagcgttta tgaaggggat tgagttaaaa tcttctctgc ataattcttc tttgaaagat
      901 actaaggttt gtattaactg acttcgtaaa gtaagtaaag tatcaatacc cctcttataa
      961 gtatctttct tttttatttg tgcctgcatt gttttattaa gttcagacca ttccttattc
     1021 atattcaatc ctctctcaaa ttctgatttt atcgccccat acaaactgga atttgtctta
     1081 caacaat
//
LOCUS       DAFGFZ010000294         1065 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000294 DAFGFZ010000000
VERSION     DAFGFZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.303"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(52..>1065)
                     /locus_tag="LR726_003124"
     CDS             complement(52..>1065)
                     /locus_tag="LR726_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL6218129.1"
                     /translation="ELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVS
                     AHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQ
                     ERE"
BASE COUNT          271 a          228 c          173 g          393 t
ORIGIN      
        1 ttcgcttgtg acggcttttc tgctaaaata acggttttca tcatgacgat ttcactccct
       61 ttcttgttgt ctgccttgtt gaaacaacat ggcacgtttt tgctttctga tttcttgttg
      121 ctggatctca tgcttcaatt gatcgttcca gtcctttttt acttcaggta taaattcatg
      181 ttcatgattt ttatagcctt tgtagtattg ttctgcttta taagagaaaa gaatctgcgc
      241 attaatatcc cctttttctt gataaaaacg atcaaaatcg tacgtaccat ccacgcgttc
      301 acaaactttt aattcttttg cgacttctct acaaatatct ttcaaattaa tggcttctat
      361 tgaatcgtta acagaaagct gctggccaaa aaacgcattt actttgtgag cggaaaccag
      421 ttggttcgtt aattgcagat tgttttctgt cttgtgtatg gccataatag cttctggtgt
      481 gaccttttct tcttttgcga cttcttcata aatgattttg tattcttttg gcacttgcca
      541 atcattagcg attcctcgtt cacaacgtac tttctgattc gtaaatggat cgactgcgcc
      601 cattaattgt tctttttcgt agaaaatatg tcctgcacgg tcattatcca cacaaatctc
      661 gatggattga ggaaaggctt gtttctttct caattcagat actgcctcac caaaataatg
      721 actgatcaca tggtgtttta acccttccat agagactaac cacgtatcgt ttaactgatc
      781 acgattcagt gcagcatagc taagtaaatc aatactactc tcaaaaaatt ttaactgctt
      841 agggtcaccg atctttaggt taaagccatg attcgctgtc gaatttttat ctatatgctt
      901 atatgtgcct ctctcaccaa aacgttcata atcaatgaac gttccttgat aactcgcccc
      961 tactgtctct tttgtctgtg gatctttcca taaaaaggcg accgcatgat ctaaacgatc
     1021 gtctcgaact aagaaatttt gccgttcata ttttggcttt agttc
//
LOCUS       DAFGFZ010000295         1063 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000295 DAFGFZ010000000
VERSION     DAFGFZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.304"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(37..993)
                     /locus_tag="LR726_003125"
     CDS             complement(37..993)
                     /locus_tag="LR726_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218130.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aac
//
LOCUS       DAFGFZ010000296         1030 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000296 DAFGFZ010000000
VERSION     DAFGFZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.306"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            9..851
                     /locus_tag="LR726_003126"
     CDS             9..851
                     /locus_tag="LR726_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL6218131.1"
                     /translation="MYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLIHIVL
                     EGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEI
                     GVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNE
                     GIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYI
                     SEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGI
                     INGE"
BASE COUNT          387 a          137 c          171 g          335 t
ORIGIN      
        1 aaaattctat atatttagta acagaaccta acactcagct gaaagattgt tactttaatt
       61 tttgtgggta ttcaaaaaca gaacctagac atagttttgg accagctatt cgagaacaat
      121 acttaataca catagtgcta gaaggtgaag gctattattc aattaatgat caaaaatatt
      181 ttctgaagaa aggccaagga tttgttatcc caccaggagt atctactttt tatcaagcag
      241 atgaaaaaaa tccgtggtca tatgtatggt tgggtttaag tggcaatctt gtagaatact
      301 atctaaaaga aattggtgtt ggaaagaatc atcttacctt taaagtcgaa aatttaaatt
      361 catttaaagg attaattttt gaaactttat cgtatgatca ggacaatcta gtcagtgaat
      421 tgtccagaca aaaacaagtt tataaatttt tagaactcct cttaaattca gctagttatc
      481 tgaacgaagg gataattact aaaaagatga atccttttgt tcgtcaaaca attgaaataa
      541 tacgtgagaa tgcgtatcaa aaattatctg ttaaagatat tgctgtcagt ctatcgatac
      601 acgaaaatta tttatcacga ttgtttaaga atgatatggg aatatcaata aaagagtata
      661 ttagtgaaat caaaatttct attgcaaacg atctgttatc atcaactgaa tattcaatcc
      721 agcaaatcag tgacaagata ggattctcaa cagtacaatc tttttctaaa gcctttaaga
      781 aagcaaatgg tgtttcaccg ggagtatatc gaaaacgtag attaggtcta ggaatcatta
      841 atggtgaata aactaaacat ttgattttga taaatattcg ttactttaaa cgcttactat
      901 ttatgtgagc gttttttata atgcaataaa gaaacggagg agagcttatg tataaggata
      961 atgaaatgaa tattatttat cgatcagaaa accaacaatt tcatttgttt aatcaggaga
     1021 taagttacat
//
LOCUS       DAFGFZ010000297         1002 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.307, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000297 DAFGFZ010000000
VERSION     DAFGFZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.307"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            369..1002
                     /locus_tag="LR726_003127"
                     /pseudo
     CDS             369..1002
                     /locus_tag="LR726_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAFGFZ010000298          999 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.308, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000298 DAFGFZ010000000
VERSION     DAFGFZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.308"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(61..279)
                     /locus_tag="LR726_003128"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="LR726_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="LR726_003129"
     CDS             complement(545..739)
                     /locus_tag="LR726_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218132.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="LR726_003130"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="LR726_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          342 a          173 c          150 g          334 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaa
//
LOCUS       DAFGFZ010000299          996 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.309, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000299 DAFGFZ010000000
VERSION     DAFGFZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.309"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..435
                     /locus_tag="LR726_003131"
     CDS             <1..435
                     /locus_tag="LR726_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL6218133.1"
                     /translation="EKDYWGESKGFIYPDSNMGGNWKATNPKKEQEAMKLKNTKSNNL
                     LYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSGGQNISSVSYETAL
                     LEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            455..>996
                     /locus_tag="LR726_003132"
     CDS             455..>996
                     /locus_tag="LR726_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBL6218134.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADE
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLR"
BASE COUNT          382 a          128 c          188 g          298 t
ORIGIN      
        1 gaaaaagatt attggggtga gtcaaaaggg tttatttatc cagattcaaa catgggaggg
       61 aactggaaag ctacaaatcc caaaaaagag caagaagcca tgaaattaaa gaatacaaaa
      121 agtaataatt tattatatgc tacgtgtaaa cattttcgac atgtacgaga tacagaattt
      181 acgagttatc atctttcagg aatagtaatt gacagtttcg tatatgaggc tatgggaaat
      241 tggaaatttg ttgaaaataa cagtgggggt caaaacatta gttctgtatc ttatgagaca
      301 gctttattag aatactataa ctcacataaa gttatgggcg gtttaaattt atactctcct
      361 ggaagcaatc aatttgttaa ttctgatagt agtatcatat gtttagaaaa agtattaaaa
      421 aaaatagctt tataaagttt tatggaggaa caacatggga gacgaaaaaa agcgcttgga
      481 agttcaatta cgtgaagcat atggacgagt aacgtatact tacaccactc atttaaagca
      541 agccgataat attatttctg acaataaaat tttaaggtgt cttcaaattg gtttgtcagc
      601 ccttactact ggagggatac ttagtaccat tattactgat aatagtgagt taacctttgt
      661 tgctggatta tgttcaacaa tttctcttgg attaaactta tactttaaga actttgatct
      721 tgctgaagaa gctaaaaatc atcaaattgt tgccgatgaa ttatggttga tcagagaaga
      781 atacgtaagc ttactaacag atttaaatcg tttaagtgtt gaagatattg tgaaaaaaag
      841 agatcaatta atgaacgaaa ctcatgaagc atataaaaat gcccctaaaa caaatagtaa
      901 aagctatgaa tcagctcaga aagcattaaa atctgaagaa gagcaatttt ttacagaaga
      961 ggaattggat aagatgttac cagagcatct tagaaa
//
LOCUS       DAFGFZ010000300          992 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.310, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000300 DAFGFZ010000000
VERSION     DAFGFZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.310"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            34..>992
                     /locus_tag="LR726_003133"
     CDS             34..>992
                     /locus_tag="LR726_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL6218135.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVY"
BASE COUNT          354 a          180 c          201 g          257 t
ORIGIN      
        1 gatcaaatgg agtctgtcaa aaaagaaagt tatttggacc ctactgtttt tgaagcagag
       61 gaacatatcc aaaaagtcat tcataaaatg ttccgtccag acaatgccta tacaaaattt
      121 gattttcgaa atggccgtcc aacgaagtct gcgatcttaa ccacgagttc cattgatatg
      181 gccaaaaagt attatcgggc catcaaagag atgacgaaag aatctgattg gttaataaaa
      241 gagtttccca atcagcctat ccgtgaagga cgaacgatgg aggatcctga tttcccacga
      301 atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca acaagaagaa
      361 atgcaaaaaa ttattgagga atataatcag tattatgata ctgcctggtc actagcagac
      421 attgagcgtt ataacggaga catcaataat cgtttggcac gaaaaagagc ggaattcaaa
      481 caatttggga aacaaatcga tttagtaatc gtagtggatc gtctattgac aggttttgac
      541 gcccctacga ttcaaaccct atttgtggat cgtaatctag agtatgcagg attgatccaa
      601 gccttttcac gtacgaaccg tacgtatcct gaaaaaacaa aaggactaat tgttactttc
      661 cgaaagccag cgacaatgga gaagaatgtc gaggatgcga caaaactgta ttcagaagct
      721 aaagaagaat cgggtttgat ttatccaagt taccaagaat ccaaaaaaag attcaagcaa
      781 gcatatggta agttaaaaga ggtttcatcc atggaatcca ttgatgaaca tacgccactt
      841 gaaatccgta tcgagtacgt caaagcattt caagaattga atcgtgcgta tgaatcgtta
      901 gtgacttatg atgattacaa tgatgacatg gtgcaatctt caactttgaa caatcaaatt
      961 caacttttgg aagaacaaat tggcgtgtac ga
//
LOCUS       DAFGFZ010000301          978 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.311, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000301 DAFGFZ010000000
VERSION     DAFGFZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.311"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            124..474
                     /locus_tag="LR726_003134"
     CDS             124..474
                     /locus_tag="LR726_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218136.1"
                     /translation="MSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYI
                     ADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIG
                     IFEKNTSEEKNLKL"
     gene            630..>978
                     /locus_tag="LR726_003135"
     CDS             630..>978
                     /locus_tag="LR726_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBL6218137.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          411 a          108 c          196 g          263 t
ORIGIN      
        1 gtaccaagag gagcatgcct attacttgca ttatgtttga gcttaagcgc agttgggtgt
       61 caaaaaagta atgaaaacaa taaaataaaa gaaggacaat ccataagttc tgaagaggaa
      121 ggcatgtcaa ttaatgcaaa tggcaaaaat gaaactttta agccatcaga ttatacattg
      181 gaggcaaaga aagaatatgt ttatgaatat ttagggctta aatttaaact ctcagacaaa
      241 tttagaaatt atatagctga taaaaagata gcgatgttag atgatcaaag tcctatagat
      301 aaagaactaa agtatgctat tttgacattt gagaaaatga cagaagaaca aaagaatgca
      361 gttatagaaa agatgggcga tggatataaa aattggcaaa atgagcttga aagaattggg
      421 actattggta tatttgaaaa aaatacatca gaagaaaaaa atctaaaatt ataaaatgtg
      481 atactcatac taaaatagga gtttcaagtg atggaaaata cgattgctat ttaagcacaa
      541 atagcggagc tgaaagtaat cttctggatg aattaaaaag aactgagatt caaattatag
      601 ataaaaaaga acgtcctaaa aatggttttg tgctatcaga aaaaactgat ttagaaaata
      661 cagaagcatt taacaaagaa tcggttaaag atttacgtaa actaccaaca aaggacataa
      721 atggcaagga ttttacaagt aaagattttg agaagtatga tcttactatg gtaaatgtat
      781 ttgcaacttg gtgtacagct tgtgtaaagg aaattcctga tttggttgag gttcagaatg
      841 aaatgaaaag caagggtgta aatatcgttg gagttgttac agatcctgtt gatgataacg
      901 gtgaaaacaa ggaagcaatt gaaaagtcaa agttaataca tgaaaagaca aaggcttcat
      961 atcctttctt aatgccag
//
LOCUS       DAFGFZ010000302          974 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.312, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000302 DAFGFZ010000000
VERSION     DAFGFZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.312"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(24..97)
                     /locus_tag="LR726_003136"
     tRNA            complement(24..97)
                     /locus_tag="LR726_003136"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Ile,seq:cat)
     gene            complement(652..966)
                     /locus_tag="LR726_003137"
     CDS             complement(652..966)
                     /locus_tag="LR726_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218138.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          359 a          152 c          153 g          310 t
ORIGIN      
        1 aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga ccggacggtt
       61 atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac ataaagccat
      121 aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct tggagtgtga
      181 ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga gacagataaa
      241 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
      301 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
      361 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
      421 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
      481 catgttcctt caaataagtt ttttaattat aaactctaga gaaattaatt catcctaaca
      541 taaaataatt gttcactcaa aagtttttct agaacaatta aaacagcagg cttcacattc
      601 gctatacatt tgtaagaagt aataattcct gtactaaaag gcataattgc tttaattatt
      661 ttttgacttc tcttcaaaca caagaagtag ttgtgtaata attgcagaaa taaggattcc
      721 aataatagat ataaaatttg acatggaaac gtctataaca ttaaaagaat aaagtgttga
      781 agaaagtaaa ctgattgaaa taacccctcc gtaaaaaata gtctgtgaat atacccactt
      841 tttttcagaa gacgtacttc gtttggttct aaaacctatc cctgcatttc tactcttatc
      901 aaaaaaccaa atatttagaa gtaataatat tgctattatt aagataaaat tactgtaaaa
      961 atccaaaagc tatc
//
LOCUS       DAFGFZ010000303          957 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.313, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000303 DAFGFZ010000000
VERSION     DAFGFZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.313"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(125..355)
                     /locus_tag="LR726_003138"
     CDS             complement(125..355)
                     /locus_tag="LR726_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218139.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(449..787)
                     /locus_tag="LR726_003139"
     CDS             complement(449..787)
                     /locus_tag="LR726_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218140.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          311 a          191 c          108 g          347 t
ORIGIN      
        1 aaaagttaaa ataaaatact ctggatactc tttatactgc attaaagtta gtaatattaa
       61 cagcaaagat atcgaaaaaa caataacata atatgtgtca aaatttttca taataagaac
      121 tcccttactg ttgcatacta acataatcca aaaactcttc tacttcagct aaagacattc
      181 cactttcttt agtccaaaaa acagtttttt ctatcgatcc ttctctgtaa actgtaaatg
      241 tcggagtcga gttaattgag tagcgactct tgaattcttt ccactcattt tcatcttctc
      301 taacttttgc tacattcacg taataaatat tttcttttcc cttttctacc agcatatcga
      361 atagcttcct gcttttgatg atcgatttct tgtgctacat ctagtaaatt ttcatcaatt
      421 tctatatcat aaagtatttc actagtgtct acatccacaa tatatacttt ttatcagtag
      481 caatttttga gtaatccgtt aaaatgatct cagtctcgtc actttcatca gtataaatat
      541 acgttaacaa tgaactcacc ttttcaatcg gtgttagctc taagcctact gtatttttta
      601 ctattgagtc attatttacc accataactt cgtactgtat ataccatcct atttggtttc
      661 catcaatatc agtaattggc tcactagcta atggagtctc cggaacaata ttttcctgat
      721 atttcttaaa ctcttctgta gcacgaaaaa ggttatcatg ttcctcactt gaaactggtt
      781 gagtcataga aaatgttact tggctaaaca gagggaaaat aaacactaca atcaacaaag
      841 tataaaaaaa agcccttctt tttcattttt tatcttccaa tcacaaattg aaaaacttat
      901 tcctgcaact ttgagtctag tatcacaatc gactgactac aactattcat ttacatt
//
LOCUS       DAFGFZ010000304          943 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.314, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000304 DAFGFZ010000000
VERSION     DAFGFZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.314"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>943
                     /locus_tag="LR726_003140"
     rRNA            <1..>943
                     /locus_tag="LR726_003140"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAFGFZ010000305          924 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.315, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000305 DAFGFZ010000000
VERSION     DAFGFZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.315"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            14..>924
                     /locus_tag="LR726_003141"
     CDS             14..>924
                     /locus_tag="LR726_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL6218141.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNY"
BASE COUNT          311 a          107 c          156 g          350 t
ORIGIN      
        1 ggagccatat gatatgataa ttaatcgtaa acaaaatgct aaaaggaata taattactgg
       61 aacaattttt aagttatatc agattatcat tccctttttt atgcgtacac ttatgattta
      121 tcagatggga atggagtatg ctggattgaa caacctgttc acatctatat ttcaaatgtt
      181 aaatttagcg gaattgggaa taggtgctgc actaactgta agtatgtata aacctatagc
      241 agaagacaat agaaaagaga tttgtgcttt actgaacgca tataaaaagt acttttcaat
      301 tattggatgg atagtgttta cattaggatt gttatgcatt ccttttttga gatttttagt
      361 taaaggttcg attcctggaa atttaaatat gacagttttg tatttaatgt atttgataaa
      421 tactgttttg tcgtattggt tattttcata tagaaagagt ctaatatatg cacatcaaag
      481 aaatgatgta attaacgtgt ctatgctaat tacttttaca ttccaatttg taatgcaggc
      541 atatgttctg attttcttaa aaaattacta tttgtattta ttggcttcta ttttgggaca
      601 aatagttctt aacttaatgt gtgctgttgt ttctcaaaag atgtatcctg attatactcc
      661 tgttggtgag ttagagaaag aaactattga cgatattata aaaaaagtaa aaggattagt
      721 tacaaacaag gtaggcggta caatattacg atcttcagat tcaattatta tttcatcttt
      781 cttaggtctt tcagtacttg cgatttatca gaattattac tttattattt ctgccataat
      841 gagtatattt gcaataattt atgaatcatg tatagcaggt ataggtaaca gtcttattgt
      901 agaaagtcaa gaaaaaaatt atat
//
LOCUS       DAFGFZ010000306          891 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.316, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000306 DAFGFZ010000000
VERSION     DAFGFZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.316"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..328
                     /locus_tag="LR726_003142"
     CDS             <1..328
                     /locus_tag="LR726_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL6218142.1"
                     /translation="EFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKR
                     SFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKN
                     TKGEIL"
     gene            325..>891
                     /locus_tag="LR726_003143"
     CDS             325..>891
                     /locus_tag="LR726_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL6218143.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTG"
BASE COUNT          300 a          100 c          184 g          307 t
ORIGIN      
        1 tgagttttat aaatcgtttg gtaatcttca ttatcagtat tttcatcata tttggttatt
       61 tatagaagtt ggaatattag gagtttcgat attcctattt tttataataa agacattttt
      121 caatgcaaaa agatctttta tgaaggataa agatgcaagt cttgttaaag ttattgcaat
      181 tatgtcggta ttcttttttg tgtataacac aacacttagg ggtgaaacaa cggggccact
      241 gatttatatg attttagcca tgcctgaagt tgtgaaaagg aaagagcatt attatagtga
      301 aaaaaataca aaaggagaga tattatgatt cctaagaaga tacattactg ttggttcggg
      361 ggaaatccac ttccgaaaaa agatagaaag tgcatagaaa gctggaaaaa gtattgccct
      421 gattatgaga ttattgaatg gaatgaatca aattatgatt taaataagaa ccgttatatg
      481 gccgatgcat ataaagaacg taaatggggt tttgttccag attatgcaag atttgatatt
      541 atttataatc atggtggatt ttatttagat acagatgttg agctaattaa gtcgttagat
      601 atacttcgtg aaaatcatgc ctatatggga tttgaagggg aaatctgggt taatggcggt
      661 attggttttg gtgcagaaga aggacattct gttattaaag gtttaaggga tatttacgat
      721 tcattaagtt tttatcaaaa tgatggtaca ttgaacttga aaccgagtcc gtattatatt
      781 acagattatc ttgaatctat tggattggtt cgcgataaca gcgaacaaat aataaatggc
      841 ctgcatatct atcctacgga ttattttgct gccaaggatt atgatactgg a
//
LOCUS       DAFGFZ010000307          864 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.317, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000307 DAFGFZ010000000
VERSION     DAFGFZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.317"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            92..706
                     /locus_tag="LR726_003144"
                     /pseudo
     CDS             92..706
                     /locus_tag="LR726_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          322 a          134 c          152 g          256 t
ORIGIN      
        1 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
       61 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
      121 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
      181 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
      241 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
      301 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
      361 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
      421 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
      481 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
      541 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
      601 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
      661 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
      721 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
      781 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
      841 tcgtccttat ttaaaggcat ttaa
//
LOCUS       DAFGFZ010000308          846 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.318, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000308 DAFGFZ010000000
VERSION     DAFGFZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.318"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            308..>846
                     /locus_tag="LR726_003145"
     CDS             308..>846
                     /locus_tag="LR726_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HBL6218144.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIF
                     DFNALKKIAKEKDTMIFKNT"
BASE COUNT          311 a           94 c          159 g          282 t
ORIGIN      
        1 aaactatgtt gttaataaaa tatcaccaat tattgattcg acaggtctat gtttattaga
       61 tgaattcttg gcggatatca gtctatttat aatgtttatt gatgtaattg aagaaacatc
      121 aagaaatttt ttgctgtcta agttcagaaa atttcgtatg aaaaatgata aacaaaaatt
      181 attggcaaat gagatttgga gtagtttaga agtcgaagaa agtaaacaag aacttttgtt
      241 gaaatggata ctttttcttg taaagaagtt ctatgtgaaa ataccagaaa cccattgtct
      301 gatagttatg cacggtaaaa taacagcttc tgcgatcgca agggaaacaa ataagttgct
      361 gaatacatat gtttatgagg ctttcgatat gccaattgaa ggtgagacta gtgacttaat
      421 taacctaatc aacaattttt gtaaaagtat agatacgacg aatggactca ttctgctagt
      481 tgatatggga tcattggaac aaatgtatga aaaaattgaa tccaatgtaa taggtgattt
      541 ggtaatttta aacaatgttt ctacagcttt agcgattgaa tgtgggattc aagtttgcca
      601 aaaaaagcca attagtcatt tttaccagat ggattttgat tcgtttcaag ttaaagttca
      661 gtattataaa ggattatcac aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg
      721 gatttctgaa aaagttaagg atattttaaa acggtatctc gataatcaag tggagatttt
      781 gatttttgat tttaacgcat taaagaaaat agcaaaggaa aaagatacta tgatttttaa
      841 gaatac
//
LOCUS       DAFGFZ010000309          845 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.319, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000309 DAFGFZ010000000
VERSION     DAFGFZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.319"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            4..>845
                     /locus_tag="LR726_003146"
     CDS             4..>845
                     /locus_tag="LR726_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218145.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIG"
BASE COUNT          267 a          112 c          123 g          343 t
ORIGIN      
        1 tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc atttgttagt
       61 ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta tgatacggca
      121 tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat taattttgaa
      181 acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac tctatatcct
      241 ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt tctttatatt
      301 gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac caaaaaacat
      361 gttactagct cctttttcgc tattatatat acaacatctt cttttaggag tgtagaaata
      421 tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct tattttactc
      481 gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc gattagcatg
      541 actttattaa tttacactca tgttctttct gtagctatga ctatggtaat gattataatt
      601 gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat tattaaatta
      661 ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc tatgattgag
      721 cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa aagagcaatt
      781 tatctgggta aagagttttt gattggaagt atagattcag aattactatc atttggtatt
      841 ggctt
//
LOCUS       DAFGFZ010000310          839 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.320, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000310 DAFGFZ010000000
VERSION     DAFGFZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.320"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(26..98)
                     /locus_tag="LR726_003147"
     tRNA            complement(26..98)
                     /locus_tag="LR726_003147"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:His,seq:gtg)
     gene            complement(101..174)
                     /locus_tag="LR726_003148"
     tRNA            complement(101..174)
                     /locus_tag="LR726_003148"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Trp,seq:cca)
     gene            complement(181..261)
                     /locus_tag="LR726_003149"
     tRNA            complement(181..261)
                     /locus_tag="LR726_003149"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Tyr,seq:gta)
     gene            complement(263..338)
                     /locus_tag="LR726_003150"
     tRNA            complement(263..338)
                     /locus_tag="LR726_003150"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Phe,seq:gaa)
     gene            complement(374..446)
                     /locus_tag="LR726_003151"
     tRNA            complement(374..446)
                     /locus_tag="LR726_003151"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Asp,seq:gtc)
     gene            complement(456..528)
                     /locus_tag="LR726_003152"
     tRNA            complement(456..528)
                     /locus_tag="LR726_003152"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(493..495),aa:Val,seq:tac)
     gene            complement(544..615)
                     /locus_tag="LR726_003153"
     tRNA            complement(544..615)
                     /locus_tag="LR726_003153"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(580..582),aa:Glu,seq:ttc)
     gene            complement(630..719)
                     /locus_tag="LR726_003154"
     tRNA            complement(630..719)
                     /locus_tag="LR726_003154"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Ser,seq:gga)
     gene            complement(759..832)
                     /locus_tag="LR726_003155"
     tRNA            complement(759..832)
                     /locus_tag="LR726_003155"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(796..798),aa:Asn,seq:gtt)
BASE COUNT          236 a          229 c          191 g          183 t
ORIGIN      
        1 ttggctatac cccaagaata aaaagggcga atgatgggaa tcgaacccac gaatgccgga
       61 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc agcaggaatt
      121 gaacccacac taacggtttt ggagaccgct gttctacctt taaactatgc ccctataaaa
      181 tggaggagag tggattcgaa ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt
      241 tagccacttc gctactcctc catggtggcg cgggacagaa tcgaactgcc gacacacgga
      301 gcttcaatcc gttgctctac caactgagct accgcgccat attttaaaat tattaattga
      361 ataacaagaa cgacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
      421 catgttaacc cctacaccac ggaaccagtt cattatggag gttaacggga tcgaaccgct
      481 gaccctctgc ttgtaaggca gatgctctcc cagctgagct aaacctccaa cattcaatta
      541 aaatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
      601 cttgaccaac gggccaggta aatttgctac ggagagtaag ggattcgaac ccttgagaca
      661 gtttggcacc atctacatga tttccaatca tgctccttcg gcctctcgga caactctcca
      721 gagtgaaaga agctcaatac tctttctcaa aatgagaact ccggcagtag gactcgaacc
      781 tacgacatca tgattaacag tcatgcgcta ctaccaactg agctatgccg gaataacat
//
LOCUS       DAFGFZ010000311          828 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.321, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000311 DAFGFZ010000000
VERSION     DAFGFZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.321"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..808
                     /locus_tag="LR726_003156"
     CDS             <1..808
                     /locus_tag="LR726_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL6218146.1"
                     /translation="VHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPE
                     IYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQ
                     TSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPL
                     ISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKI
                     MLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          312 a          103 c          136 g          277 t
ORIGIN      
        1 agttcattat ttttttactg gaaaaataga agatctattc catgaaccgt taattgaata
       61 tacagaaaat ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa
      121 ttacgatcca gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa
      181 tggagatatt cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag
      241 tgttagtgga gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt
      301 agcacaaacg tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc
      361 tttgatacaa gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc
      421 tgggattgtt tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt
      481 aatttcttct gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga
      541 aatagctaat tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat
      601 gcatattacg atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt
      661 gaaatcaaac catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca
      721 cttttcgaga gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt
      781 agtggacaat ttcaacaaca ttagctgaga ggttttattt tattatga
//
LOCUS       DAFGFZ010000312          823 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.322, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000312 DAFGFZ010000000
VERSION     DAFGFZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.322"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            22..>823
                     /locus_tag="LR726_003157"
     CDS             22..>823
                     /locus_tag="LR726_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL6218147.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DAFGFZ010000313          818 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.323, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000313 DAFGFZ010000000
VERSION     DAFGFZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.323"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            60..293
                     /locus_tag="LR726_003158"
     CDS             60..293
                     /locus_tag="LR726_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBL6218148.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            290..697
                     /locus_tag="LR726_003159"
     CDS             290..697
                     /locus_tag="LR726_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL6218149.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          310 a          116 c          144 g          248 t
ORIGIN      
        1 tgatttaaaa tcaacaagcg tatatactgt atatacaaag aaaaaggagg gagaagacta
       61 tggcaattaa agaaagaaaa tttagggaag tggggaactc agtagtatta actatttcaa
      121 aggagtttct taaagaaaat gggttaaatc ccggagatac ggttttttta gatgaagaaa
      181 aacttcgagc ggcaattaca aaaaaacaag ccttatctga tgaaattgat ttagcaatat
      241 ctcaagcatt aactgaatat gatactactt ttagagagct tgtcgataaa tgagtattca
      301 ttacctagaa gccaaagata ttataaaaat gaatgtaatc caaataaaaa aatactctcc
      361 taatgaaccc cttggtgtaa aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg
      421 agcctcagct tttggtgaag aggtttatcc ttcgatttat gaaaaagcag ctatcctact
      481 tatacaattg ataaagaaac atccttttca caatgcaaat aaacgtactg ctttcttagc
      541 tacttttgtc tttttgaaaa tcaatggaca gcttttagct attgatcaaa aagatgctgt
      601 aaatttagcg gtatatattg ctacgtatga taaggatttc gatcaactaa aatatgaagt
      661 gatcaacact ataaagacac atacgcagcc catttaagga gttactgtat gtgtctttat
      721 agggttatta gctgctatag cgaaataatt gaagtaaatt acaaatagct ataattcaaa
      781 tccaactata tagaaaacgt aaattttcta tatacttg
//
LOCUS       DAFGFZ010000314          802 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.324, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000314 DAFGFZ010000000
VERSION     DAFGFZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.324"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..724
                     /locus_tag="LR726_003160"
     CDS             <1..724
                     /locus_tag="LR726_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218150.1"
                     /translation="KKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNIIE
                     EIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAID
                     NVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPPT
                     WKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKKD
                     YSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT          305 a          110 c          144 g          243 t
ORIGIN      
        1 gaaaaagaaa aaattattat ggatttgttt ggcaatttta ttgataattg gaggaattga
       61 gatatttacc acatatagat taaaaacgaa tcagcctaaa caatatgtat cagacatgtt
      121 aaatattatt gaagaaatag actcagaaat gaaagctttc tcaaaaactt ctatggattt
      181 gggagaaaga tatgatttta aggggtttat ggaagataaa atggacgagg tatcaagtag
      241 ggaaattcca gaaggcacac ctaaagctgt tagggacacc aaagatactc ttcttgaagc
      301 gattgataac gtgaacacag ctttactaac taatgatgta aaattattag aacaaggcca
      361 gctttgtttt agtatagctc aagccatata tacagagttt ttagatggaa atataaaaga
      421 gaagtcactt aatgaaaaac ttaattcatt tgtagaaaca tacacagcta taccacctac
      481 ttggaaactt gtaaatgaag ataaaggaat cacgataaat actgatacag agatttctac
      541 agatgctcaa tctttatcag atttattagc taatctaaca ggtatcagta atgcagtgac
      601 agagaaagtt ggtaaagata ttccacttcg gttacttgaa aatgggaaaa aagattattc
      661 tgtggtattt aagaacggac aggtaactga ttacaacgaa gtatttaaaa aagttattga
      721 ataatgattc ttccatcttt ttcatttatt taccctaccc cgttgttata tgaccgtact
      781 tatacatctc cagtagctta gg
//
LOCUS       DAFGFZ010000315          797 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.325, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000315 DAFGFZ010000000
VERSION     DAFGFZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.325"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            117..290
                     /locus_tag="LR726_003161"
     CDS             117..290
                     /locus_tag="LR726_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218151.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          265 a          130 c          100 g          302 t
ORIGIN      
        1 tacaataaat aactttagtt actttaatgc gttcttactt tatttttata ttatcatttt
       61 tcttattcac ttctttggtt tatactattc ttataaataa aaacgaggta tgtcctatga
      121 tgaaaagatt gtactattca ttgatcataa cgattggcta tttgattgta tcaaatctag
      181 gaaatatggt atttggtatc agtaaagaat tcagttggac tactacatta tgggaatctc
      241 tattcttttt catcttcgta tttttgttac aaaattatcg aaaaaaataa acatgtgcca
      301 tctatctgtt ggcacatgtt ttaggattta tggtactttt tattcggcaa tccattttat
      361 ttttctactt cttcttaata cctctaacac ttttgattta taaactaatc caatgcatct
      421 acttgcgctt attaagctat ttcagctaga tagtaccacc ccatgcgtca atgcttgatt
      481 ttccttttgt ataacattgt ataagcaata aaggacaaat aagtggtgat tacaccaaac
      541 cattgaataa gaaaaattct gatacgtaaa taaacatgaa cttgaaaaaa ttaatgaatc
      601 ggttagctac tacgtagttg tcttttttct catacagttc tgttaactta cacttctctt
      661 cgctagataa taaaaaatcc tccaactgta ttgtccaata taatcccagg catctaaact
      721 agcattatag aagagacaca catagaatct aaaataactg gttttaaatg tttcttacaa
      781 tcaaaaaaag ctgttaa
//
LOCUS       DAFGFZ010000316          794 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.326, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000316 DAFGFZ010000000
VERSION     DAFGFZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.326"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            25..>794
                     /locus_tag="LR726_003162"
     CDS             25..>794
                     /locus_tag="LR726_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HBL6218152.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTREL"
BASE COUNT          297 a          115 c          147 g          235 t
ORIGIN      
        1 ttgataacaa gctacgtgac gtatatgcct aacaaaaata gaacttttga atcaatctta
       61 aatgaaggtt taaccacaga accggcatcc attatttttg atgaagttat cgatgacgtt
      121 gaagatagta agtatcataa aaatttaaaa gaactttttt attttgcact caacaatgga
      181 ccaagtttgt tcactggtat atcagtacct gatagtgata atccattaat tatggcaaaa
      241 atttacttaa caaaatggtg taataaatac atcagggata ggaataaccc agcattaaaa
      301 aaacctctta aaacatttgg cgaaaaagat gctgctttaa ctactagagt agcagcaaat
      361 gctaatattg atgatcaaaa agtattgaat gactacttgc gtggacattt tttatatatg
      421 agcgctgaga acatgaacgg aagtatttta gaggaatatt tagccgaagt gttagaacct
      481 gaaggttgga tttggtgcgc cggctctgta tatcgagctg ttgatttctg ttatttagga
      541 acctcaccaa ttcttttaca agtgaaaaat aaatacaata cagaaagcag ttcgagttca
      601 gctataagag taggaacaac aattagaaaa tggaatcgtt taaataagtc gacaaaaatt
      661 tctggtttgg atagccctat ccccaattgg aaagcattaa tagaaatgac tgaagctagt
      721 aaggaactgg cagctaagtt aactgaaaat agttatttag cttatattaa tgaaaaatcc
      781 acacgagaat tatg
//
LOCUS       DAFGFZ010000317          787 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.328, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000317 DAFGFZ010000000
VERSION     DAFGFZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.328"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..760)
                     /locus_tag="LR726_003163"
     CDS             complement(<1..760)
                     /locus_tag="LR726_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218153.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTH"
BASE COUNT          313 a          121 c          130 g          223 t
ORIGIN      
        1 tatgtgtcag catatcacta ggagccatac cacttaattc ttttcccagt gggactttca
       61 attcttgtgc tctagattgt tgaataaaag gaattaagaa tcccgcactt aaaaaaaagg
      121 tgaggacggt agcttttaaa agacttaaga gtctagcgat tttttggtcc caaaaataaa
      181 aagaaagaaa caacgttcct ccaataaata cagaaaccat agcgacagaa agtaaatgag
      241 tatacactac tagcgtcatg ccaatagaca accagtacca tttttggtaa tctctaatat
      301 aaatttcgta acaccccatt agtatcaaag gcaaaaatgt taaagcgaca gcttctccca
      361 atgaagcacg atggaatatg tcaatggcac gataggcgga aaatgtataa ataatcgaaa
      421 ataacagcga aatataacga ttgtttttta tttgtttcat agaaaaataa gaaacgacca
      481 ttgttaaaaa agtgataaaa aaatagtaga tattataact taaaacaaga tttcccatca
      541 ttttataaaa taaaaaagct ggataaagag taagccatgg ataaaaaata ttcatcattg
      601 ttccatgatg atcgaagtta ttgaagttta ctggagaact ccatacatta tctagtccta
      661 tgattcgaga taaatgaaag taagtatcct gattgacact atgatagatt cccttagaca
      721 tgaatagagg gatgacgtaa gcaaaactta gaaataacat aaaaatgata tctattattt
      781 ggttttt
//
LOCUS       DAFGFZ010000318          773 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.329, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000318 DAFGFZ010000000
VERSION     DAFGFZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.329"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..254)
                     /locus_tag="LR726_003164"
     CDS             complement(<1..254)
                     /locus_tag="LR726_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218154.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSV"
     gene            complement(384..>773)
                     /locus_tag="LR726_003165"
     CDS             complement(384..>773)
                     /locus_tag="LR726_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6218155.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          249 a          123 c          120 g          281 t
ORIGIN      
        1 aatactgata acttataatt atatcgtcca ttgacaatca aatataaact aactatactt
       61 gttagaacaa aaaaacaggc gttgacttgt tcttgattag ttaaaaatat caataaagga
      121 atacttaccc aatatatttt tctatttatt tcttctcctc ttaagagttg aaaaaacgga
      181 taaaaagtta gtaaactaaa tgctactggc cactgatagt tcaaggtggt tgctctccaa
      241 ccggtttccc ccattactgt caatggtatt aaacagtata tagagcaaac caataataaa
      301 tttttaacat ttattttctc aaaaacatac tgggagcgtc aataattttg tgtaaataaa
      361 ttgtcctcct gcaaaataat tagttactca gtaaacattg aaactaatgt atcggttacc
      421 tgttgaaaac ctttatggct tctgtttaga aatttttgat tgtatgtatc aaaaatgctg
      481 actagaaagc gttctagtga ttcttcattt tgaaactgct cttttctacg gctgtatctt
      541 ttaatttgct tattgaaaga ctcgattaga ttggttgagt aaatggttct acgaatgcta
      601 ggtggaaaat cataaaaagt taataagtct tggttttcta tgagtgactg cgtcacttta
      661 ggatagtttt tcttccattt ctcaatcatg ccggataaga aggtattcgc ttcttctttt
      721 gagttagctt gataaacagc cttaaagtca tcacagattt cttttcggtc ttt
//
LOCUS       DAFGFZ010000319          765 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.330, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000319 DAFGFZ010000000
VERSION     DAFGFZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.330"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..742
                     /locus_tag="LR726_003166"
     CDS             <1..742
                     /locus_tag="LR726_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6218156.1"
                     /translation="SNKYVSVIKSKLNLLFSYAMKKGYVEKNPIDEVVIDYKRESKTI
                     KIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDVHITNNNA
                     SVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQ
                     TTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGH
                     ENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT          302 a          114 c          127 g          222 t
ORIGIN      
        1 gtcaaataag tacgtcagtg taattaaatc caaattgaat cttctttttt catatgctat
       61 gaaaaaaggt tatgtagaaa aaaatcctat cgatgaagta gttattgatt ataaaagaga
      121 gtcaaaaacg ataaaaatta aagataaatt tttagaagat gatgaatata atagactagt
      181 agatttcaca acttcacaca ataaaagata ttctctcctt ttccagtggc tatatttgac
      241 tgggatgagg tctggtgaag caatcgcatt aagtaaagat gatgtacaca ttaccaataa
      301 taatgcatca gtagttataa atgggacgat gatgtataga gaacgttcaa tagctgatat
      361 gaaaaaatct gattctacaa aaacagctgc cggaatgcga gaaattgatt taccaaaaaa
      421 agcgatagct atttacaatg agcttctaga attaaatcca aatggtcaat ttctatttca
      481 aacaacgaaa ggaactcctt tccaactaac agcaattaac acctatttga gaagtcataa
      541 agctgacatg aagattgata aaaaacttag ttcacatatt tttagacata cccatatttc
      601 aaaattagcg gaactaggaa ctcctctgta tgccattcag gatcgcgttg gtcacgaaaa
      661 tagtgatatc actgaaaaaa tttatttgca tgtgacaaaa ggagtaaaag aaaaattgaa
      721 agaagatata gaaaaactgt aacttgttgc cccttttttg cccct
//
LOCUS       DAFGFZ010000320          748 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.331, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000320 DAFGFZ010000000
VERSION     DAFGFZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.331"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            183..692
                     /locus_tag="LR726_003167"
     CDS             183..692
                     /locus_tag="LR726_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218157.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT          278 a          123 c          136 g          211 t
ORIGIN      
        1 ctaagtaaag attgacgatt tgttgcttga attcttgtga aaaagttctt cgtgttctct
       61 tagacataaa aattcctcct ggtatgtttt cttctagtct acacacctta atttttctgt
      121 ctagttaatt gtagcctatc caaaagtaag gaattacaaa aagagaaaga agagaaggaa
      181 gaatgaaaaa ggtattattt gcggttagtt tagttgttaa tatagcacta gcaggaggat
      241 tattttatac cattacagaa atgaataagc ttgatgacca acgggaaagc ttagcaatca
      301 gtaacgaaca atacaaaagg aagctagatg aagctagcag caccaacgtg gacactcaag
      361 aagccgtaga atcctctaca attgtttata gcaatgaatc cagtgaaacc ctaacaaatt
      421 cggaaagaag cacagctcca gctacagaaa ctgtttctca aaaagctact ataacaatag
      481 gaagtaaagt attcacgttt atagtaaatg gcttgacaat agaacacgat tctagcagaa
      541 caggaaaaat gaccgagctt tctcatgagt ttccagtgat tgttactact cctagtgcta
      601 cccttaacgg tatattggtg caagacaaca atgataataa ttgctatata atggtagacg
      661 gtcaaactct atacagagga acataccaat aaattttgag tcttaatatg actgatattt
      721 ataaaaaatt cctgatatta gaacaaga
//
LOCUS       DAFGFZ010000321          738 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.332, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000321 DAFGFZ010000000
VERSION     DAFGFZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.332"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            45..443
                     /locus_tag="LR726_003168"
     CDS             45..443
                     /locus_tag="LR726_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218158.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKYT
                     SMKPCILDWQWLIDAVLDVKCSKYNITLGC"
     gene            446..619
                     /locus_tag="LR726_003169"
     CDS             446..619
                     /locus_tag="LR726_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218159.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT          273 a           81 c          126 g          258 t
ORIGIN      
        1 ttatgatatt ataaaaacat aaattatatt aaggagtgat cctgatgcga ttgagaagaa
       61 ataagaatta cactaccatt attttagaag attttactga tcaagaatta cttattgtcg
      121 gtaaaacaaa agcagatttt atcgaaaagg gaaatgcttc aatagtaaaa gcttcaaagg
      181 ttgctgtcga aaaatatttt gattctttag aagaaagtgc taaggaaatt tttaatggag
      241 agaaagctgg tagctatgag ttagacggta ttagatttag cttgatccca acaactgaaa
      301 atattattcc gaagaaatta tattatatct tttcatctga aaaatatact tcaatgaagc
      361 cttgtatttt ggattggcaa tggttgattg atgctgtatt ggatgtaaaa tgttcaaaat
      421 acaatattac tttggggtgt taattatgag tatgctagtt agaaaattac cattatctgt
      481 aagagaattt ctattttact tggtctgctt aatcgcatca gggtttaagc tgatcgaaca
      541 tatttttatc tttgcttatg tattagcaat gatcgcattt ttatctggcg gttggttaat
      601 tatttatttg ttaagctaag ttttagatca aaaataatta ataaattaaa cgataaaata
      661 accgttaata aatgtaatga aagggcaacg cctaacaatc agcttgtaat aagattataa
      721 aataacataa tatcaaaa
//
LOCUS       DAFGFZ010000322          736 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.333, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000322 DAFGFZ010000000
VERSION     DAFGFZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.333"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            74..697
                     /locus_tag="LR726_003170"
     CDS             74..697
                     /locus_tag="LR726_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL6218160.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
BASE COUNT          278 a          117 c          128 g          213 t
ORIGIN      
        1 ctttatagtg tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa
       61 gtaggtaaaa attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca
      121 acgccaaatt gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc
      181 tggagcaacg attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca
      241 agatattttt attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc
      301 ttcagtcaat tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat
      361 ggctgaagca attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat
      421 tttggccatg attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat
      481 taaaattgca aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac
      541 aaaaaatcct caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat
      601 agctatttca aaaattgcta aaaactacaa tattactaga caaactattt acagaattaa
      661 gaatgatatt gagtataaca gtaatcaaat gaattaaaaa agttctttat acattaaaat
      721 ttcttctttt aactca
//
LOCUS       DAFGFZ010000323          726 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.334, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000323 DAFGFZ010000000
VERSION     DAFGFZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.334"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..387
                     /locus_tag="LR726_003171"
     CDS             <1..387
                     /locus_tag="LR726_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218161.1"
                     /translation="IISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVS
                     EIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHY
                     AVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            401..>726
                     /locus_tag="LR726_003172"
     CDS             401..>726
                     /locus_tag="LR726_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218162.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDV"
BASE COUNT          267 a           99 c          131 g          229 t
ORIGIN      
        1 ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa taaagtaatt
       61 aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt ttctgtaaca
      121 gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat tgaagtttta
      181 aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt gcctcctcaa
      241 gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga agcaggagag
      301 cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt tgatatcaag
      361 gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata tagtaccatc
      421 aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt gcgcatcggt
      481 aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta aaaatgataa
      541 tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg atggcaaaat
      601 ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa aatatgatga
      661 agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt cctcagatta
      721 cgatgt
//
LOCUS       DAFGFZ010000324          717 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.335, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000324 DAFGFZ010000000
VERSION     DAFGFZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.335"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            157..543
                     /locus_tag="LR726_003173"
     CDS             157..543
                     /locus_tag="LR726_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6218163.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            637..>717
                     /locus_tag="LR726_003174"
     CDS             637..>717
                     /locus_tag="LR726_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218164.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFS"
BASE COUNT          262 a          114 c          113 g          228 t
ORIGIN      
        1 atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct
       61 tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc
      121 ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta
      181 aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat
      241 aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt
      301 actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt
      361 gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt
      421 atcgaaaaat ttatctttga taaaactgat aaagaaattg gaaaggttct ggggattaca
      481 agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt
      541 taatatctgc ataataacta attttgtatg cttctttgct cgttctcctc cttcccaaag
      601 aaatagatta taagtccatg ggaagaaggt agatttatga attctttaaa agaagatttc
      661 attttagcaa aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag atttagc
//
LOCUS       DAFGFZ010000325          690 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.336, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000325 DAFGFZ010000000
VERSION     DAFGFZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.336"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            203..>690
                     /locus_tag="LR726_003175"
     CDS             203..>690
                     /locus_tag="LR726_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218165.1"
                     /translation="MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSL
                     TLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIE
                     LDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYT
                     KLA"
BASE COUNT          232 a          112 c           92 g          254 t
ORIGIN      
        1 cttatttctc ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt
       61 atttattttc tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca
      121 actttttaac catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc
      181 ttctttattt atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa
      241 taattcatgg aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt
      301 aagcaattct atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt
      361 tgctatagct gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc
      421 tgatatgatt aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca
      481 ttttatgagt aacaaactta caattgaatt agataattcg aataataaaa atactgtctt
      541 gacgacacct atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca
      601 aacaaaatta tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt
      661 cgttattact tatcagtaca caaaactagc
//
LOCUS       DAFGFZ010000326          689 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.337, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000326 DAFGFZ010000000
VERSION     DAFGFZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.337"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            82..>689
                     /locus_tag="LR726_003176"
     CDS             82..>689
                     /locus_tag="LR726_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBL6218166.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DAFGFZ010000327          676 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.338, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000327 DAFGFZ010000000
VERSION     DAFGFZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.338"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(233..382)
                     /locus_tag="LR726_003177"
     CDS             complement(233..382)
                     /locus_tag="LR726_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218167.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          227 a          109 c          100 g          240 t
ORIGIN      
        1 attatacaga taataacgga atattatcat cgaaacagct aacaaaattg tcataaaaaa
       61 cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa gagagacaat
      121 ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt tacaaacgtc
      181 ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact ccttatctag
      241 attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg ttattttcgt
      301 tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg cggtattgca
      361 tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt agttcttgaa
      421 agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt ttgatgcaat
      481 taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc tattttcttt
      541 taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa tataaatcat
      601 atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg aacaacatag
      661 gaatataaac aaacgt
//
LOCUS       DAFGFZ010000328          666 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.339, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000328 DAFGFZ010000000
VERSION     DAFGFZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.339"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>666
                     /locus_tag="LR726_003178"
     CDS             <1..>666
                     /locus_tag="LR726_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6218168.1"
                     /translation="NITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVK
                     RINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVE
                     GKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFD
                     GHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDL
                     GKL"
BASE COUNT          290 a           73 c          127 g          176 t
ORIGIN      
        1 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
       61 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
      121 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
      181 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
      241 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
      301 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
      361 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
      421 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
      481 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
      541 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
      601 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
      661 aattag
//
LOCUS       DAFGFZ010000329          657 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.340, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000329 DAFGFZ010000000
VERSION     DAFGFZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.340"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..367
                     /locus_tag="LR726_003179"
     CDS             <1..367
                     /locus_tag="LR726_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL6218169.1"
                     /translation="FGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFG
                     RVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGK
                     ENWNDLYHANEVLKIAEEK"
     gene            368..>657
                     /locus_tag="LR726_003180"
     CDS             368..>657
                     /locus_tag="LR726_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL6218170.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFL"
BASE COUNT          256 a           73 c          120 g          208 t
ORIGIN      
        1 atttggtttc cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc ttgaaattaa
       61 cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag aaattcattt
      121 agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag atgaatgtat
      181 caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta aagaaatgcc
      241 tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa acgtagtaac
      301 aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa tagctgagga
      361 gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg ggtttaaaaa
      421 taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag caatgataga
      481 tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata cggacagtaa
      541 tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc
      601 tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat tcttaca
//
LOCUS       DAFGFZ010000330          656 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.341, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000330 DAFGFZ010000000
VERSION     DAFGFZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.341"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LR726_003181"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LR726_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL6218171.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="LR726_003182"
     CDS             complement(481..>656)
                     /locus_tag="LR726_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218172.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAFGFZ010000331          652 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.342, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000331 DAFGFZ010000000
VERSION     DAFGFZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.342"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            73..>652
                     /locus_tag="LR726_003183"
     CDS             73..>652
                     /locus_tag="LR726_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218173.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTR"
BASE COUNT          232 a           94 c          126 g          200 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg tt
//
LOCUS       DAFGFZ010000332          650 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.343, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000332 DAFGFZ010000000
VERSION     DAFGFZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.343"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            6..>650
                     /locus_tag="LR726_003184"
     CDS             6..>650
                     /locus_tag="LR726_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL6218174.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGS"
BASE COUNT          245 a           74 c          128 g          203 t
ORIGIN      
        1 aagttatgtt agaagtgaaa aagcttaatg tttgttataa cgatttttta gcattgaacg
       61 agatttcttt aaaaatacaa gaaggtgctt taacaggtat tattggtcca aatggtgctg
      121 gaaaatccac attgcttaaa gcaatgttaa atattatccc tcatcaggga gaggtctcca
      181 ttaataatga agatatcaat aaaaaactgg tgaagattgc atatgtagag caaaaagctg
      241 atattgattt tacttttcca ataaaagtaa aagaatgtgt atctatggga acatatgccg
      301 gaatgaaagt ttttcaaaga attaaaaatg cagaatggca aagagtttca aaggctttgg
      361 aaaaggtcga tatgggaaaa tattctaatc atcaaatagg agaattatct ggaggacaat
      421 ttcaacgtgt acttttagca agatgtcttg ctcaaaatgc ggatttcata tttttagatg
      481 aaccatttgt agggatagac ttagttagtg aacgaataat tatggatacg ttaaaaaaat
      541 tgaaacatca gggaaaaaca gtgctaattg tgcatcatga tttaagcaag gtaaagaagt
      601 attttgataa tattattctt ttgaatcgaa atctaattgc tcacggaagt
//
LOCUS       DAFGFZ010000333          649 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.344, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000333 DAFGFZ010000000
VERSION     DAFGFZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.344"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..428
                     /locus_tag="LR726_003185"
     CDS             <1..428
                     /locus_tag="LR726_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL6218175.1"
                     /translation="IMNPKVEYIYEFEGGKGITNQYRPNKFETFKEFYSLANTVTDDI
                     NKDGMAALLLKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAK
                     KITKLLYNMPAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            447..>649
                     /locus_tag="LR726_003186"
     CDS             447..>649
                     /locus_tag="LR726_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL6218176.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKK"
BASE COUNT          233 a           90 c          132 g          194 t
ORIGIN      
        1 tgatcatgaa cccaaaagta gaatatattt atgaatttga aggtgggaag gggatcacta
       61 atcaataccg acctaacaag ttcgaaacat ttaaagaatt ctattcatta gcaaatactg
      121 taacagacga tattaataaa gacggtatgg cggcgcttct tttaaaaggg acaacttcag
      181 ttattttttc tatttatagt acctctgcaa gaacttcaaa agagaagaaa gacgaagcaa
      241 agcagatatt gagtgatcca gtgatccgag aagccaagca aattgctaag acggattctt
      301 cagcaaaaaa aataacaaag ttgctttaca atatgccagc tccactattt ttatctgctg
      361 taaaagttgg aagcttcgtt gaagtgaaaa tgccagggtt aatggctgct ttgaagcgag
      421 tatattagtt aatggaggaa aatataatga aaagaatttt gcattttcag ggacgaatgg
      481 gtttaggtgg agcagagtct ttcatgatga atttatatcg taaaattgat cgtacaaact
      541 atcaatttga ttttttgatt tatgaagatt atgcagatgt tcaagactat cattcagaga
      601 ttgaaagatt aggaggccgt atttttgttg tacctaatcc taagaaaaa
//
LOCUS       DAFGFZ010000334          615 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.346, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000334 DAFGFZ010000000
VERSION     DAFGFZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.346"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>615
                     /locus_tag="LR726_003187"
     CDS             <1..>615
                     /locus_tag="LR726_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL6218177.1"
                     /translation="YYLKELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSI
                     EVPYIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKII
                     RPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMEL
                     YKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYIN"
BASE COUNT          246 a           69 c          110 g          190 t
ORIGIN      
        1 tactatttga aagaattaat tgatcaagtt ctatttgtta aaaatagaac tgtaaaaact
       61 acaactattc agttgttaaa aactgaaaac aataacgcat taaggaaatt tgacattttt
      121 aatagttcga ttgaagttcc ctatatcgaa aaggtaagtg cagatgtatt aaaagttgaa
      181 gaaattactt atgaaaatat tctaaaagac ataaattttg atgtcaaagt tggagatgtt
      241 attggtttgg tagggaaaaa cggtgtgggg aaaacaacct tactaagaat actcatgaag
      301 attattcgcc ctaccaaagg aaagataatc gaaaataaaa aaaatttgtc atcgccgttt
      361 ttagtaatgc aagaaatgga ttaccaattt ttcacagaat cagtgaggag cgagttagcc
      421 ctggggaatg aaatagttag taaagatcag caagaaaaaa ttttaaaaaa aatggaactt
      481 tataaaatga aagatcaatt accgtttgat ttatccggtg gagaaaaaca aagattattg
      541 gtttctatag cttcattgtc aaaaactaat ttatttctat ttgatgaacc tacaagtggg
      601 ttggattata ttaat
//
LOCUS       DAFGFZ010000335          612 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.347, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000335 DAFGFZ010000000
VERSION     DAFGFZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.347"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            48..>612
                     /locus_tag="LR726_003188"
     CDS             48..>612
                     /locus_tag="LR726_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL6218178.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVI"
BASE COUNT          245 a           52 c          118 g          197 t
ORIGIN      
        1 taatatgctg tataatatct agtgtatagg cttaagtgga ggtatatatg aaaatattaa
       61 ctgttgaaga tgataatatg ataagagagg gaataagtga atatctttca gaatttggat
      121 atactgttat tcaagctaaa gatggaagag aagccttgtc aaaatttaat agcgatataa
      181 atttggttat cttagacatt cagatacctt ttataaatgg tttagaagtg ttgaaagaaa
      241 ttagaaagaa aagcaattta ccaattctaa tcttgactgc atttagtgat gaagaatata
      301 aaattgatgc atttactaat ttagtagacg gatatgtaga aaagccattt tcattgccgg
      361 tcttaaaagc tagaatagat tctttaatta agaagaatta tggacattta gagaagtttg
      421 aatataagga tctatcggtt aattttaata gctatacagc taaaataaat ggtgaagaaa
      481 tagatgtaaa tgcaaaagaa ctagaagtat taaaatgttt attggataat aatggacaag
      541 tgttaacaag aatgcaaatt attgattatg tatggaaaga tagcgaagaa actccttatg
      601 atcgagtaat ag
//
LOCUS       DAFGFZ010000336          612 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.348, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000336 DAFGFZ010000000
VERSION     DAFGFZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.348"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            315..605
                     /locus_tag="LR726_003189"
     CDS             315..605
                     /locus_tag="LR726_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL6218179.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          207 a           95 c          104 g          206 t
ORIGIN      
        1 tgaacattat ccagcaatat gagctaaagt atataacctt tgatcagcta tcagaagaga
       61 tttggggtta tggccaacgt ttgataaatg aggttggtgt tgagcgcttc tctttctatg
      121 tagaagctgc agcaggttat cataatttta ggttttatat tttcccgtta tatatataag
      181 gtattttacg gattctatag agttatctct atgaatcctt ttttgttttt acaaatagga
      241 gtagatacta gaataatgcg tgtgttaaac tattattgca aacgcaataa taaaagaaag
      301 taggcttacg caccatgtct atcaatacat ttgtttactc gcacccgatc aatgtttaca
      361 ttatcaagaa tttaggaatc actgtagaac aattttgtga gttatatgcc tacccacaag
      421 gaacggttgc aagctggata acccgacaac gtagaataaa atcattacct gcttcattcg
      481 tttatgattt aagcctggcc tctagtttaa acatgtctga tgtttatgaa aagctcctgt
      541 ctttagaaaa agaatatgat tccttcaaga ttaaacatga taaaaaaata aaaaaacata
      601 tataatatca at
//
LOCUS       DAFGFZ010000337          593 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.350, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000337 DAFGFZ010000000
VERSION     DAFGFZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.350"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>593
                     /locus_tag="LR726_003190"
     CDS             <1..>593
                     /locus_tag="LR726_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBL6218180.1"
                     /translation="NPEEVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAF
                     NELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTE
                     AMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNL
                     LTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGF"
BASE COUNT          205 a          123 c          111 g          154 t
ORIGIN      
        1 ctaatcctga agaagtaaaa tttttagtca cagacatgaa cgccgcctac ttccagctta
       61 ccaaacgtat tctgccaaat gcgaaagtag tgattgatcg gtttcatatt atcaaacaca
      121 tgaatcaagc ctttaatgag ttgcgtatcc gtgaaatgaa tgaacttcgt aaagtaggac
      181 agaaaagtca ggcagaaaaa ctgaaaaaga actggcgctt tttgctaaaa aatcgtgcaa
      241 acatcaacca ttatgaatac aaaacatgga aaagttttcg agcaccaaaa tacccatttc
      301 ttacggaagc gatgatgatt gatcgattgc ttggcttttc tacgtccctg aaggaggcgt
      361 atccctattt tcatgaatta gttgaagcct ttcgagacaa agaccctgac ttatttttct
      421 ccttattggc agaacttccc gaaacgttag atgacggctt tcgggaaaag ctccaaaacc
      481 ttctgaccta tgaagaaggc atcaccaacg cgatgatcta tccttacacc aatggaaaaa
      541 tagaagcgaa gaatacccat ataaagacaa tgaaacgagt atcctacgga ttt
//
LOCUS       DAFGFZ010000338          591 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.351, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000338 DAFGFZ010000000
VERSION     DAFGFZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.351"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            26..>591
                     /locus_tag="LR726_003191"
     CDS             26..>591
                     /locus_tag="LR726_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBL6218181.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          210 a          101 c          117 g          163 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DAFGFZ010000339          578 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.352, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000339 DAFGFZ010000000
VERSION     DAFGFZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.352"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..175)
                     /locus_tag="LR726_003192"
     CDS             complement(<1..175)
                     /locus_tag="LR726_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit R1E"
                     /protein_id="HBL6218182.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKIN"
     gene            complement(334..558)
                     /gene="nrdH"
                     /locus_tag="LR726_003193"
     CDS             complement(334..558)
                     /gene="nrdH"
                     /locus_tag="LR726_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL6218183.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
BASE COUNT          167 a          108 c           96 g          207 t
ORIGIN      
        1 aattgatttt ttccataata gatagaaaag tcatcgtatt tggtttgaca ttttcctcaa
       61 aaaaggcaac taaagcttct ttatccttat tcaaagggat ttgaccattg actggccgat
      121 tgatttcatt attcagcttg aaataactga catcttgtaa atttttaaga ctcatacgta
      181 cgcccaacct tcatttcgta tttgatgatg cttgattgca aatcattgca ttgtttttaa
      241 gctttaaaaa aggatttgga agtttgcgac aaaaactggt gctgtctcaa gcccttcaaa
      301 tccatgtatc acgttttgat tcttttagtc atgttagctg gcaagtttac gcaactgatc
      361 aggacggaat ccgataactg tttctgcatc agaagtaatg acaggaacac tttggaatcc
      421 ttgtgctttc aaccagtcta ctgcttcagg ctgttcatcg atattgactt cttcaaaagc
      481 aatttgattt tctgttaaaa accgtttgac catcttacat tgcatacaat tatttttaga
      541 aaagattttt acgttcatgg tgagtgcctc ctctaaca
//
LOCUS       DAFGFZ010000340          551 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.353, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000340 DAFGFZ010000000
VERSION     DAFGFZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.353"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>551
                     /locus_tag="LR726_003194"
     CDS             <1..>551
                     /locus_tag="LR726_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL6218184.1"
                     /translation="CNIFTEINLLNEYYDEIIEVAKKKDRLLYLSSTFLKRKEIQYIK
                     SKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGK
                     VKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILI
                     EWEGNPDSLFSWNEETSSMTDIQ"
BASE COUNT          205 a           65 c           93 g          188 t
ORIGIN      
        1 ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa taatcgaagt
       61 agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga gaaaagaaat
      121 tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat accatgtagg
      181 acagtattta cccgactggc atccttggga agattatcgc aatttttttg tttcaaacaa
      241 aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta tagatacatt
      301 tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg atattgatta
      361 ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa ctttaagtgt
      421 agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa agattttaat
      481 agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt ctagtatgac
      541 agatattcaa t
//
LOCUS       DAFGFZ010000341          531 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.355, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000341 DAFGFZ010000000
VERSION     DAFGFZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.355"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(28..426)
                     /gene="spxA"
                     /locus_tag="LR726_003195"
     CDS             complement(28..426)
                     /gene="spxA"
                     /locus_tag="LR726_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL6218185.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          154 a          109 c           87 g          181 t
ORIGIN      
        1 gtcttttcca agtttcgtat tatccttcta taatccagcc atcaattgtg cttgacgtaa
       61 ttctaattgt cgtacatcgc gcggtaggaa ccgacgaatt tcatcttcat taaaaccaac
      121 ttgcagacgt ttatcatcaa tcatgattgg acggcgaagc aatccaggat tgttttgcac
      181 aagttccaat aattcttgta acggcagatc atctaaatcc atgttcagtt tttggaatac
      241 tttggaacgt gtcgagatga tttcttctgt tccgtcttct gtcatttgta aaatcgcttt
      301 taattcagaa ctgttcaatg gttctgaaaa aatattacgt tcaacaaatg gaatctgatg
      361 ttcttgcaac cacgcacgag cctttctgca ggaagtacaa cttggggaag tatataatgt
      421 taacaacgtg caccactcct tttcgtgata agaatcactt ttttgtaaca gaagatcata
      481 ataatctccc atctgtttct attatacata atcaactttt aaatagctac c
//
LOCUS       DAFGFZ010000342          525 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.356, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000342 DAFGFZ010000000
VERSION     DAFGFZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.356"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(43..345)
                     /locus_tag="LR726_003196"
     CDS             complement(43..345)
                     /locus_tag="LR726_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218186.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          181 a           92 c           72 g          180 t
ORIGIN      
        1 tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt tatacctttc
       61 tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc gtgcgtctgg
      121 cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg tttttgttgc
      181 cggaatttta aagtagttct tattagagta atagagattg attaataagc tatcttcgat
      241 tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa atctattcga
      301 aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt tcacctcaaa
      361 aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata atatggatgg
      421 aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt ttcattcatt
      481 taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaa
//
LOCUS       DAFGFZ010000343          514 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.357, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000343 DAFGFZ010000000
VERSION     DAFGFZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.357"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..132)
                     /locus_tag="LR726_003197"
     CDS             complement(<1..132)
                     /locus_tag="LR726_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL6218187.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAFGFZ010000344          511 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.358, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000344 DAFGFZ010000000
VERSION     DAFGFZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.358"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>511
                     /locus_tag="LR726_003198"
     CDS             <1..>511
                     /locus_tag="LR726_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL6218188.1"
                     /translation="RNTDQLKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGD
                     FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLN
                     QIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNI
                     LGDKGYISHK"
BASE COUNT          172 a           93 c           92 g          154 t
ORIGIN      
        1 agaaatactg atcaactgaa gcaacgcgat acagtgatta ttgcttgtgt tatatggggc
       61 ataattaatg gctatactag ccaaagagcc acgtatagag cggtttgttc cgtcttattt
      121 cctaatggtg actttcctag taggagtcga ttcactcgct taagttcaaa cttagcttac
      181 actatcaaga ttattcgata ctttttcatc aagaagctca ccaaaggtga actagtcgga
      241 attatagata gttttcctag tccattatgt aaaccagtta gaaatagaca agcaaaacta
      301 ttgaatcaaa tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt
      361 aaaattcata tgatcgttac taagactggc tttccgatta cttactcaat cacgaatcca
      421 ggtgtccatg acgtgaaagt gttagagact ttatcggaag aagcgaatct tcccaatatc
      481 ctaggggaca aaggatatat tagccataaa a
//
LOCUS       DAFGFZ010000345          469 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.359, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000345 DAFGFZ010000000
VERSION     DAFGFZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.359"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
BASE COUNT          163 a           65 c           81 g          160 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggataacg tgattttttg ctgtaatgac
       61 aaggttttag ctgtttcaga taccatgtat ccaaaaatag tttattgaga aaataggaga
      121 gtatttgata ctaactagta ggaaaaaatg agataaaaag taaatggaat catttcaggt
      181 aatctcaaat aaaaaaatat acagtcctgt ggcactttac ctatgggact gtatatattt
      241 tttactctca gcgggcttgt tttgactccg tgtctgactc cgtcgtattg attttaaatg
      301 atattcgatg accatttaac aggtcatttt tgaaattttt gtctctatat gactcagttt
      361 gataattatt gatagatcaa tttgggtcta tatcgaacaa gggaataatc caataaaaaa
      421 tgatttagaa tatcaaaata acaaaaaaga ggactacctg tattagtcc
//
LOCUS       DAFGFZ010000346          460 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.360, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000346 DAFGFZ010000000
VERSION     DAFGFZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.360"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..203)
                     /locus_tag="LR726_003199"
     CDS             complement(<1..203)
                     /locus_tag="LR726_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218189.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEV"
BASE COUNT          164 a           73 c           64 g          159 t
ORIGIN      
        1 tctacctcta atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt
       61 gcagttacag gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc
      121 tggaatttgt ctttttcgtt gctttgctca taaaaatctg catattcatc actaatattt
      181 tttctaataa attcttccat cataaagatc acctcgaaaa gagtatacga acaaacgttc
      241 tttttgtaaa ggtggaattt attatactaa ataaataggt gaacaagtac ttgtgccaaa
      301 ttatgtgcca aaaaaaatcg aatttaataa aacacaaaca aaaagaaatc ctattatgat
      361 aacatttctt ataataacaa acacaacaga agacgtgtaa tagttagtca ggaacgtaca
      421 aataacccct gtatcctttg cggtacaggg ggttattttg
//
LOCUS       DAFGFZ010000347          460 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.361, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000347 DAFGFZ010000000
VERSION     DAFGFZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.361"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..193)
                     /locus_tag="LR726_003200"
     CDS             complement(<1..193)
                     /locus_tag="LR726_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218190.1"
                     /translation="MNNKNTKNTNTNHEKRSFFDKLFKKKKNGKVSILKMIGWIFLAF
                     FIVAVNSSGGDKKEAASKDT"
BASE COUNT          124 a           84 c           74 g          178 t
ORIGIN      
        1 tggtatcttt tgaagcagcc tctttcttat caccgccgct actgttcaca gcaactatga
       61 aaaaggctaa aaatatccag ccgatcattt tcaaaataga tacttttcca ttcttcttct
      121 ttttgaaaag cttatcaaag aagcttctct tctcgtggtt agtattggta tttttggtgt
      181 ttttgttgtt cattcttaaa tctcccttaa tttaattttc aaacgctctt aggaacgttt
      241 ttaacgtttc ttatggttgg ttttggtttg gctggtgtgc catttcacaa cttttctagc
      301 aacttctcta gttgtgatcc cgtgtatttt gcactctatt attggaaaaa atttctctga
      361 agtaaaccct acacaaacgc caattagttc ttttggttct gttgcaaagt tttaaatcta
      421 ctatcaaata aggtagaata atagaaaaag atagcaggag
//
LOCUS       DAFGFZ010000348          459 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.362, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000348 DAFGFZ010000000
VERSION     DAFGFZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.362"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            36..>459
                     /locus_tag="LR726_003201"
     CDS             36..>459
                     /locus_tag="LR726_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HBL6218191.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYE"
BASE COUNT          183 a           55 c           85 g          136 t
ORIGIN      
        1 ataagcagtg aatttataat caacgtatag gagacatgga tgaaatgaaa ctaattggta
       61 tagatttaga cggtactctt ttgaattctg accagagcat tagtcaaaaa aatgcaagaa
      121 caatgaaaca tttaccggaa gactgttttc cattcatttg ttctggaaga gaagtagaag
      181 acattaataa tattctcaaa aaatctggtt taaggatacc agcagtaggg ttaaatggtt
      241 ctgttggatt tgatggagaa aaaaagatat ttgagtcttc cttcgataaa aatgatgtaa
      301 aaaaaattga ttctgtccta tcgaaatttc caaccaaaat atatacaaat ctcggaagtt
      361 atgaatctca gaattataaa aataaaatga aaaaaatttt tcacgaggta gggagtgaat
      421 tttctatcaa agagctaaac tatgagcttg agtatgaaa
//
LOCUS       DAFGFZ010000349          458 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.363, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000349 DAFGFZ010000000
VERSION     DAFGFZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.363"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
BASE COUNT          139 a           93 c           73 g          153 t
ORIGIN      
        1 tttgtaccaa agtttgaaca tttaaccgcc tgttagacag ggctgttaat ttttcccact
       61 cattcacgat tgttcttttt ttactcatat tcagttcctc ctcaaagaaa aaagagattt
      121 ttggaaagaa tttccagagt tcacgtaact ctaaaaattc ataaccatca atctctagtc
      181 ttgaagaaac ctaaaaatat tcgtataatg aatttgagtt acggaactag tgtggttagt
      241 ttccgtaaag gtcgagaacg ttggtagcgt tgctcgactt atttttttgc tctttttcca
      301 gttcatttgt ttgctaactc aataatagca cttctcttta gctatgcaaa taccaaaaaa
      361 ataaaaccgc accctttgaa aagggcgccc ccaaacttcg ctaacgctaa gttggccaaa
      421 aaatggactt gccatttttt agcaaatgtc agcttcag
//
LOCUS       DAFGFZ010000350          455 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.364, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000350 DAFGFZ010000000
VERSION     DAFGFZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.364"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>455
                     /locus_tag="LR726_003202"
     CDS             <1..>455
                     /locus_tag="LR726_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL6218192.1"
                     /translation="ITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKPLNY
                     SYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDDHQL
                     QLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEV"
BASE COUNT          169 a           53 c           93 g          140 t
ORIGIN      
        1 tataacatta ggttcacaaa aatttcaatt tgatagattg ttaaaagcta ttgatgattt
       61 aattgaaaaa ggagtaataa tcgaaagtac atttgcacag agtggttata gtgattataa
      121 accactaaat tattcctata aagcatttct aaatagggat gagtttactg aaactatgca
      181 aaaagcagaa atggtgatta ctcatggagg tacaggtgct ataattgggg ccgtaaaaaa
      241 aggtaaaagg gtcattgctg ttcctcgatt ggctaagtat ggtgagcatg tcgatgatca
      301 ccaactgcag ttaattagac aatttgatga gatgaatatt atctatcctt gttatgaatt
      361 agaaaattta gaagatgccg ttaaagaaac aagagttcgg atgtttaaca agtatgtatc
      421 taacacggaa acgattatta attcgataga ggtat
//
LOCUS       DAFGFZ010000351          450 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.365, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000351 DAFGFZ010000000
VERSION     DAFGFZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.365"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            98..292
                     /locus_tag="LR726_003203"
     CDS             98..292
                     /locus_tag="LR726_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218193.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          191 a           57 c           77 g          125 t
ORIGIN      
        1 gattcttttt atttatcata aagatataga tacataatca ttttcactta catatatttg
       61 aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag aagtaaaaac
      121 aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc ctttacttgg
      181 ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa agaacgagct
      241 agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt aaatccaatg
      301 tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt atagaattag
      361 aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac catatgatta
      421 tttcaatatg tatcctggaa ttggagatct
//
LOCUS       DAFGFZ010000352          439 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.366, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000352 DAFGFZ010000000
VERSION     DAFGFZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.366"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(34..270)
                     /locus_tag="LR726_003204"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="LR726_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a           74 c           74 g          147 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgcta
//
LOCUS       DAFGFZ010000353          418 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.367, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000353 DAFGFZ010000000
VERSION     DAFGFZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.367"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
BASE COUNT          158 a           51 c           85 g          124 t
ORIGIN      
        1 tcattggtcg ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc
       61 ttttatggta gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat
      121 atgaatcgag atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta
      181 cagtttcgtt cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa
      241 gagggcggca catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag
      301 gctatcctac tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt
      361 cattaaatcc cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatct
//
LOCUS       DAFGFZ010000354          411 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.368, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000354 DAFGFZ010000000
VERSION     DAFGFZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.368"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>411
                     /locus_tag="LR726_003205"
     rRNA            <1..>411
                     /locus_tag="LR726_003205"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAFGFZ010000355          403 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.369, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000355 DAFGFZ010000000
VERSION     DAFGFZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.369"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            46..>403
                     /locus_tag="LR726_003206"
     CDS             46..>403
                     /locus_tag="LR726_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218194.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMAS"
BASE COUNT          163 a           69 c           77 g           94 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
       61 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
      121 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
      181 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
      241 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
      301 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
      361 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcg
//
LOCUS       DAFGFZ010000356          402 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.370, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000356 DAFGFZ010000000
VERSION     DAFGFZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.370"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..150)
                     /locus_tag="LR726_003207"
     CDS             complement(<1..150)
                     /locus_tag="LR726_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL6218195.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYES"
     gene            complement(169..330)
                     /locus_tag="LR726_003208"
     CDS             complement(169..330)
                     /locus_tag="LR726_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL6218196.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          134 a           76 c           42 g          150 t
ORIGIN      
        1 actttcatat aaagcctttt ctatttcatt gcttgatttc attattgtta aacaagaata
       61 caagcaagaa agaactatag ttaatatgat aaaaattatt aaagttctat ttttctttct
      121 tgtaatatat gctatagcat tttttatcac tttcctaact actcctctct attttcttac
      181 tccataacta attgcatcca ctggacaagc atttttacat cgtccacatc tgatacactc
      241 cagatgattg caattctcaa ctgaatcttt attcatttgg caaacttttt tacattttcc
      301 acaattaata cacttatcct tatcctgaat cggctacact agactagaca gaaaaaataa
      361 ggtgtgtaga ataataaaaa acacactgga ggatttttca tg
//
LOCUS       DAFGFZ010000357          399 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.371, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000357 DAFGFZ010000000
VERSION     DAFGFZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.371"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAFGFZ010000358          391 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.372, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000358 DAFGFZ010000000
VERSION     DAFGFZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.372"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..300
                     /locus_tag="LR726_003209"
     CDS             <1..300
                     /locus_tag="LR726_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218197.1"
                     /translation="NKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT          152 a           55 c           85 g           99 t
ORIGIN      
        1 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
       61 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
      121 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
      181 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
      241 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
      301 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
      361 atgaccgaaa aaaaaatgag tcttattgat c
//
LOCUS       DAFGFZ010000359          386 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.373, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000359 DAFGFZ010000000
VERSION     DAFGFZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.373"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
BASE COUNT          108 a           68 c           83 g          127 t
ORIGIN      
        1 tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggacgata aatttggaca
       61 attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctggta accttcgtcg
      121 acgtccacag gtggcattaa gtctataccc cttaatgcgt ttacctttca gtgttacggt
      181 attacccgta acggatttag tataacagat attcaaaagt gataccactt tttttggctg
      241 tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata atgttataaa
      301 aatgtccgtg gggctctttg ccgtatgacg aacgctgaaa ttagagggaa cgcatgtttg
      361 aatgcgttcc ctcttttttg tttgtc
//
LOCUS       DAFGFZ010000360          382 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.374, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000360 DAFGFZ010000000
VERSION     DAFGFZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.374"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>382
                     /locus_tag="LR726_003210"
     CDS             <1..>382
                     /locus_tag="LR726_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218198.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIV"
BASE COUNT          140 a           68 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tg
//
LOCUS       DAFGFZ010000361          370 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.375, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000361 DAFGFZ010000000
VERSION     DAFGFZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.375"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            134..334
                     /locus_tag="LR726_003211"
     CDS             134..334
                     /locus_tag="LR726_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL6218199.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          133 a           56 c           68 g          113 t
ORIGIN      
        1 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
       61 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
      121 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
      181 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
      241 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
      301 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
      361 cgaaagaaaa
//
LOCUS       DAFGFZ010000362          368 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.376, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000362 DAFGFZ010000000
VERSION     DAFGFZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.376"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            13..315
                     /locus_tag="LR726_003212"
                     /pseudo
     CDS             13..315
                     /locus_tag="LR726_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          138 a           38 c           68 g          124 t
ORIGIN      
        1 aggagtataa aaatgaacaa acataataga aaaataacga ataacaaaga actgatcttg
       61 ttactaatag ggatttgggg tatcttttta ctctttaata tgtatatgcc gactatgaga
      121 gcggatgacg tagtttatgc aagtagatta gatgaacttg gctatttggg tgcttcgata
      181 gaacattata agacatggag ttcaagaatt attatagaac tattcttaat gttcttttct
      241 aaacacttta tgctatggaa attattaaat tctacgatta tgttaggaat tgtagtatta
      301 ctttgcaagt atgttgagag tataaaatat tttgtgtaaa tgaaaaaatc catacaaaaa
      361 aggaagtc
//
LOCUS       DAFGFZ010000363          366 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.377, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000363 DAFGFZ010000000
VERSION     DAFGFZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.377"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..345
                     /locus_tag="LR726_003213"
     CDS             <1..345
                     /locus_tag="LR726_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218200.1"
                     /translation="YFLVKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSH
                     YHANGFDMGSIHKSSLLSPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANY
                     QKKFKLKLKDSF"
BASE COUNT          132 a           58 c           54 g          122 t
ORIGIN      
        1 tattttttag ttaagcatga tagaagtaat cgaatgattc ttactggcgt aattgcaaac
       61 ccatcaaaaa tgccaaactc aattatcgaa tgtacgtatc tttacaacgg taagaaaatt
      121 aattgttcac attatcacgc caatggtttt gatatgggaa gcattcataa aagtagcctt
      181 ttatctccta taccgttggc acaagcaatc tctcctggct actcagtcgc tttttcagaa
      241 gttttagata tgaaagatat attacctggt gaaaaacttg taatggtcat aagaacagct
      301 aattatcaaa aaaaatttaa gttaaagcta aaagactcat tttaattgaa aaatttttct
      361 ttaata
//
LOCUS       DAFGFZ010000364          362 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.378, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000364 DAFGFZ010000000
VERSION     DAFGFZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.378"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..>362)
                     /locus_tag="LR726_003214"
     rRNA            complement(<1..>362)
                     /locus_tag="LR726_003214"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAFGFZ010000365          356 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.379, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000365 DAFGFZ010000000
VERSION     DAFGFZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.379"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            18..>356
                     /locus_tag="LR726_003215"
     CDS             18..>356
                     /locus_tag="LR726_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218201.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSL"
BASE COUNT          142 a           62 c           61 g           91 t
ORIGIN      
        1 taaaaaggag aaaaaagatg aagccaaatc cttcagaagc aggaaaacga atcaaacaat
       61 tacgcctttc atgtggtttt actatggaag aacttggaag gaaaatagat aattcaccac
      121 gtgcgaccat atccaactgg gaacgtggga ccaatttacc aaatccacaa aagctgaaat
      181 tattatcaac tatcactaat tcaacaattg actggattaa atggggaact ttagaagaat
      241 atataacttc ataccttatc gatatagggt atgagctata tattaaggac ttcccagaaa
      301 tacctcacaa agtctttaaa gacattcaag aaagatatag tgatacgttt tcgcta
//
LOCUS       DAFGFZ010000366          351 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.380, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000366 DAFGFZ010000000
VERSION     DAFGFZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.380"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(92..>351)
                     /locus_tag="LR726_003216"
     CDS             complement(92..>351)
                     /locus_tag="LR726_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL6218202.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          107 a           54 c           64 g          126 t
ORIGIN      
        1 tattaaatag tttaaaaaaa tgaaaaataa taaaggaagt gagtcaattc cagactcctg
       61 tgtaaaatgc tatacaatgt ttttaccatt tctacttatc aaaattgatg tattttcttg
      121 aagaataaat ccattcatca tgtaggtcca taagaacggc tccaattaag cgattggctg
      181 atgtttgatt ggggaagatg cgaataatct tttctcttct gcgtacttct tgattcagtc
      241 gttcaattag attggtactc tttagtcgat tgtgggaatt tccttgtacg gtatattgaa
      301 aggcgtcttc gaatccatca tccaatgatg cgcaagcttt tgaatatttt g
//
LOCUS       DAFGFZ010000367          341 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.382, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000367 DAFGFZ010000000
VERSION     DAFGFZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.382"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>341
                     /locus_tag="LR726_003217"
     CDS             <1..>341
                     /locus_tag="LR726_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218203.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAFGFZ010000368          339 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.383, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000368 DAFGFZ010000000
VERSION     DAFGFZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.383"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            149..>339
                     /locus_tag="LR726_003218"
     CDS             149..>339
                     /locus_tag="LR726_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL6218204.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSS"
BASE COUNT          147 a           51 c           50 g           91 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtga
//
LOCUS       DAFGFZ010000369          335 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.denovo.385, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000369 DAFGFZ010000000
VERSION     DAFGFZ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.denovo.385"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>335
                     /locus_tag="LR726_003219"
     CDS             <1..>335
                     /locus_tag="LR726_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBL6218205.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          125 a           53 c           77 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgag
//
LOCUS       DAFGFZ010000370        15133 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.068, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000370 DAFGFZ010000000
VERSION     DAFGFZ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.068"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..510
                     /locus_tag="LR726_003220"
     CDS             <1..510
                     /locus_tag="LR726_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218206.1"
                     /translation="FDGSPDRVLGNFLYYEAQPHDPAVLQEALRTEQHSRGRAVPGDF
                     KVHVTALHDSRIETEARRIVDSLKDLSAPNSPNKTHFMVEISPHFAAIASTKDNDRLF
                     AMLPYKSLCFTGMKGRHGVYAVINRDERRDVSIRRPRPSIRKQLSDSKQAASPKKAAA
                     RTKKNELEV"
     gene            514..717
                     /locus_tag="LR726_003221"
     CDS             514..717
                     /locus_tag="LR726_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBL6218207.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(1017..1253)
                     /locus_tag="LR726_003222"
     CDS             complement(1017..1253)
                     /locus_tag="LR726_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218208.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(1436..1822)
                     /locus_tag="LR726_003223"
     CDS             complement(1436..1822)
                     /locus_tag="LR726_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBL6218209.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(1804..3132)
                     /locus_tag="LR726_003224"
     CDS             complement(1804..3132)
                     /locus_tag="LR726_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL6218210.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(3093..3422)
                     /gene="mobC"
                     /locus_tag="LR726_003225"
     CDS             complement(3093..3422)
                     /gene="mobC"
                     /locus_tag="LR726_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL6218211.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(3680..4051)
                     /locus_tag="LR726_003226"
     CDS             complement(3680..4051)
                     /locus_tag="LR726_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218212.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            4196..4381
                     /locus_tag="LR726_003227"
     CDS             4196..4381
                     /locus_tag="LR726_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL6218213.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            4560..4706
                     /locus_tag="LR726_003228"
     CDS             4560..4706
                     /locus_tag="LR726_003228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218214.1"
                     /translation="MTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVSRHWITAG
                     LMRL"
     gene            5324..5983
                     /gene="vanR"
                     /locus_tag="LR726_003229"
     CDS             5324..5983
                     /gene="vanR"
                     /locus_tag="LR726_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBL6218215.1"
                     /translation="MSIRILLVEDDDHVCNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5986..7329
                     /gene="vanS"
                     /locus_tag="LR726_003230"
     CDS             5986..7329
                     /gene="vanS"
                     /locus_tag="LR726_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL6218216.1"
                     /translation="MERKGVFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            7500..8306
                     /locus_tag="LR726_003231"
     CDS             7500..8306
                     /locus_tag="LR726_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBL6218217.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            8324..9151
                     /gene="vanW"
                     /locus_tag="LR726_003232"
     CDS             8324..9151
                     /gene="vanW"
                     /locus_tag="LR726_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HBL6218218.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMYQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            9148..10119
                     /locus_tag="LR726_003233"
     CDS             9148..10119
                     /locus_tag="LR726_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBL6218219.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10112..11140
                     /gene="vanB"
                     /locus_tag="LR726_003234"
     CDS             10112..11140
                     /gene="vanB"
                     /locus_tag="LR726_003234"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBL6218220.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            11146..11754
                     /locus_tag="LR726_003235"
     CDS             11146..11754
                     /locus_tag="LR726_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBL6218221.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(11820..11993)
                     /locus_tag="LR726_003236"
     CDS             complement(11820..11993)
                     /locus_tag="LR726_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218222.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            12342..12773
                     /locus_tag="LR726_003237"
     CDS             12342..12773
                     /locus_tag="LR726_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6218223.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            12780..13013
                     /locus_tag="LR726_003238"
     CDS             12780..13013
                     /locus_tag="LR726_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218224.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            13437..13637
                     /locus_tag="LR726_003239"
     CDS             13437..13637
                     /locus_tag="LR726_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBL6218225.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            13721..14914
                     /locus_tag="LR726_003240"
     CDS             13721..14914
                     /locus_tag="LR726_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL6218226.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
BASE COUNT         4006 a         3487 c         3934 g         3706 t
ORIGIN      
        1 tttgacggaa gccctgaccg ggtattggga aactttttgt actatgaggc ccagccccat
       61 gacccggctg tgctgcagga ggcgctacgg acggagcagc acagccgggg gcgggccgtc
      121 cccggcgatt tcaaagtcca cgtcaccgct ttgcatgaca gccggattga aaccgaggcc
      181 cggcgtattg tggacagcct gaaagatctg tccgcgccta acagccccaa taaaacgcat
      241 ttcatggtgg agatctcccc gcactttgcg gcgattgctt ccaccaagga taatgaccgg
      301 ctctttgcca tgctgcccta taaatccctg tgttttaccg ggatgaaagg caggcatggc
      361 gtgtatgccg tgattaaccg ggacgagcgc cgggatgtga gtattcgcag gccccggccc
      421 tctatccgca agcagctatc cgacagcaaa caggccgcca gcccaaaaaa ggcggcggcc
      481 agaaccaaaa agaatgaatt ggaggtataa ggaatgatgc actttaccgt ggaggaagaa
      541 aacctgatct gtatgtacca caatgcagac cggcgcagaa cgataaacag gctcaccgcc
      601 gtcatgccgg ttatggatgg ggatatgcgg gcgcaggccc agcagaccct ttccaagctg
      661 gaacgcatga cggatgccga ctatgacggc cagaagttcc acttcacaga cgaataacga
      721 acagcagacg ccgggcgcgg cggccatgtg gtcgcttgcg tccggcgtct tttctttttg
      781 cctattccta cgcatttaga acccggtatt gtcggggata ggatgaagta ttcgcttgcc
      841 cctgcaaaca tgcgggagaa gccttgcagc gggcggtttt caatccctta aatgcgtagg
      901 ttgcagctat aattagaccc ctgaaacaga tatagccgcg cagcgggtgt atctgtttca
      961 ggggtgtgca ggtgatagcc gcaaagcggc gtaagagggt gcagccctct tgccgtctat
     1021 ctctgttcca gcgggaagct cggcaatgct tccagcagtt tcagggaaat gcgctgccgt
     1081 atatcttcgt caatgacttt cttctttttg ccgtttgcct gtctgatctc caccgtggaa
     1141 agctcgttga tgtagtcagc ataatgggcc aacacttttt ccgtggccca cctctcaccg
     1201 gcaacggcgg ctttgatcgt ttcatagtcc agcagctttt cttgtccgtc catcctgata
     1261 cccccgtcag actgattttt tctatgatac catattcgga agcatggaac cagagaaaac
     1321 ggcgcttttc tgctggtaaa ttctacgcat ttagaacagc acttcggcgg ggataggata
     1381 aaacacccac ataccccgct gaccatgccg gaaaagcctt gaaatataag ggcttttaca
     1441 gctctaagtg cgtaggtttc cagtatcttt tccgctggcg ctcggcctct ttgcggcggc
     1501 gcatccgttt ggagcaggcc gcgcagtatt tggcccggtt agacttcggc gtgaacgtgg
     1561 ccccgcactc acagcagcgt ttcacttcgt cccggtcttt ggtgatctcg gcatacaggg
     1621 ccgcatccag cgggaggaca gcggccttaa accagcggca cagaagcgaa taggaaatgc
     1681 tctgtacgca aatacattct tcgccgtcgt ccagcaacag gcagttgccg tcacaatagt
     1741 tgcagcactc atgcgtcagc ttccgggcct tgcgatattg agggtaattc attcgtggaa
     1801 tactcatagc tcttgcgtcc attcctttcc cggcgtcata cgcaaaatgc cgtccacatt
     1861 ctgcttgatg atgccgtatt cctgcatctg ctttttcgct tcgccgtact tctcatacag
     1921 ccgttccttt tcctcggcca atcgcttgta ctcggctttc agggcgtaca gatcggggag
     1981 ctttttcaca ccctgtccct ttagggcctt tgccgcagct tcatacaaaa tcaacgtgct
     2041 ttcatgctca cgccggaatt ttgccttatc cgctgcattg cggtattcca tagccacggg
     2101 ccagagctgc ttgtaggtag aaatattctt tatcagcacc gccatatccg caagccggtg
     2161 ttccatagct ttgagggtag cggcggcctc gtcgctggcg gcggtgatct cggccacttt
     2221 gctttccaaa tccgggtagg tgtctatccc atgttcagtc aggaaattca aagtctttgc
     2281 ggcctgcttc aaattgtgga gcttcgccca cctctcatag cctgccgact gctgggcctt
     2341 aatgttgttt tccagatcaa tccgcaaaga aatccccttg cgctctttgg gcgctctggt
     2401 gataacgcgg cctttgatac gctctttgat ggcttcctcg gtgtaggctt cccccaatgt
     2461 cttgcagcgg gtaaagcgtt cctgtccggg agcgcggaaa gaaataaact ttcccggctt
     2521 gatctcatag ccctgcgcct gcatgagccg caggaaatca tcaaaatctt ttgcctgcgg
     2581 gatagcggcg tcaatggctg ctttgagctt cgctttccag ctcgtccctt tccgctgggc
     2641 gtcccactcg gcatagctct tgcccttact ctggccgggc ttcaccacgg acaatccgtt
     2701 ttccttgcac agcctgtcgc tggtgcgccg gatataggca tagctctgtt tgttggaaat
     2761 gtacttgcgc tgatttacca tatccacggc acagaaaata atgtgattgt ggacgtggcc
     2821 cttatcaata tgggtagtca gcacataggg atatttccct tgcagcactt cctcggcaag
     2881 ctgcttcccg atctcatggg cctgttccgg cgtggcttcg ccgggggcaa aggattgtat
     2941 caggtggtgg cccagattat tgcccttttg gtatgcctgc gccagcagca tttcaaattc
     3001 aatgtccgct gtttcataag agcaggcaaa ggaggccacc agcagcttgc cgtcagtttt
     3061 gtccgggttc tcgatgtagt caagggcttt tttcagcgtt gacttgatag gatgtatctt
     3121 tgtaactgcc atatctgcgc cagcgccccc tttatatcct cgatgtcctg tgcgtacacc
     3181 ggccccatac cgttcagcct ctttgcaatc tgattgacgt tgacgccgat cttctgtatc
     3241 tcggcggtct gcgcccggat gtcgctggtg tccatgttga cgatatagcc gtcaatgagc
     3301 atcttccggg cgtaggctga aaagttcttc gtatgcagca gcgccatttt ctgttcaatc
     3361 atagcccgtt cctccggcgt cacagggacg cgcagcacga tttttcgctt tctgtttgcc
     3421 atgcttcacc gtcctttcca ttcaggggtt tggggatttc cccaacaagc aaaacggacg
     3481 cggcccggca gggacggggc aggccgtttt tccgggagtg gctacactcc ctatgcttgc
     3541 tacgttactg tttctccact aacaagacgt ttccaaagcc ccaaactacc cgcaaaatag
     3601 ccctctggaa agaaaaaagt gcaaaaaaat agaagctacg ctgtgcagct cccgctaaaa
     3661 gtaaaacaga ggatgccagt tacacatcct ccgtttcatt cttcttcggc ttttcaatta
     3721 gtatggcgct gtcaatcgcg ccctcgatga tcggcaggta ttcttcgggg cagagtttca
     3781 gcttgtggct gaccctttgc cgcagttcgc tttcctcccg catgatctcc gggttaaagt
     3841 agcgttccac aggaagcccg caggctttaa taagctgtat gacaaccggc agactgggaa
     3901 tggccccgtc gttttcgatg ttggcaagat agcgccagtc aatatttatt ttctctgcca
     3961 ttgctttgcg cgaaacgcgc tttgcttgcc gggccgcttt cacatccgcg ccgaaagttt
     4021 caaagccggg acaatcttca atattcgcca tatcgcacct cccacttaca ttgtatattt
     4081 catactctgc ccgtggaatg ttggtatatg cacctttctt aaagtttata tttcacattt
     4141 tgtttctttt attcttcgag caaaagaatt ataatgtacc ctgtggaggt gtactatgga
     4201 ctacatgacg ttgaaagagg caggcgagaa atggggtgtg cccccacgat ggataaacta
     4261 ctattgtgct gcgggacgca tccccggcgc tgtaaagatg gcgacgatct ggctcattcc
     4321 caaaactgcg gaaaagccga ttgacaggcg tacaaagaaa ggaaaggcgg ttactgaata
     4381 gtataatgaa accggcgcat tttggatatg gttttgaaga gagagaaagc atggtatttt
     4441 gtcacggaaa aactataaga tgatagatgg caggctgctc cagaccgata aaaagtattc
     4501 tcatctgaaa cagaaagaaa agatttacac atggatgtat gaagaagcga aactttatca
     4561 tgacagaaat ggaaaatgcc cgaaacaaag acgaggaagc tgctgctgta tcagccgtat
     4621 atggtagaat tgaaaaggcc ggcatctgga ttctttatgg tgaagtctcc agacattgga
     4681 taactgctgg tctaatgcgc ctttgagtat ggggtgattg attttaatac cggggaggta
     4741 cggctttttg aggaaaatat tctgggattt acatttggcg attattacat ttcctgtaac
     4801 tgcaaggaca cggatgatga agatatgagg aatatagact tgctgatttt ctactgtctt
     4861 gaacggtatt aggactgaat ctgctgcgcg gagtgcggca agagcgaccg gagggagtac
     4921 acacaggccc tgcctgtgtg aaaagccata ccgcaaggtg cattcgcaaa aaagaggccg
     4981 cgcattttgc tgaggcttgg ggtgtcccca acaagcaaaa cgagaacgcc gcaggcgggc
     5041 aagtggtgga tcagaatatc cgtaaaggat attctgatcc attgcttgcc agtattatcg
     5101 ggatgttttg acaaccgctg taatcgaaac atcccaaaca ttaagaaggt gttaattatt
     5161 attatcttga tttaattcgt ttaaaccaat aatatagata catacaccat gaaaagaagc
     5221 gagtatccca atgacggatg attaaaatcc gaaaagaaat cgctttgaaa ctacagggaa
     5281 actacagact gttatgttat cttcttaaaa tggagggatt tttatgtcga tacgaattct
     5341 acttgtcgag gatgatgatc atgtctgtaa tacagtaagg gcgtttctgg ctgaggcagg
     5401 atatcaggtg gatgcctgca cagatggaaa tgaggcatac accaagtttt acgaaaacac
     5461 ttatcaactg gttattcttg atattatgct gcccggtatg aacgggcatg aacttttgcg
     5521 tgaatttcgt gcgaaaaatg atactcccat tctgatgatg acagccctgt cggatgacga
     5581 aaaccaaatc cgggcgtttg atgcagaggc agacgactat gtaacaaagc cattcaagat
     5641 gcagatttta ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc tggcaaagga
     5701 aatccgtgtc ggcaggctga cacttctgcc ggaggatttc acggtacttt gtgacggtac
     5761 agagctgccc ctgacacgaa aagaatttga aatccttttg ctgctggtgc agaacaaagg
     5821 cagaacctta acgcatgaaa tcattttgtc acgtatatgg ggatatgact ttgagggtga
     5881 tggcagcaca gtccacactc atatcaaaaa tttgcgggcc aagctgccgg aaaatatcat
     5941 caaaaccatt cgcggtgtag gataccgatt ggaggaatca taataatgga aagaaaaggg
     6001 gttttcatta aggttttttc ctatacgatc attgtcctgc tactgcttgt cggtgtaaca
     6061 gcaacactgt ttgcacagca atttgtgtct tatttcagag tgatggaatt acagcaaaca
     6121 gtaaaatcct atcagccatt ggtggaactg attcagaata gcgataggct tgatattcaa
     6181 gaggtggcag ggctgtttca ctacaataac caatcctttg agttttatat tgaagataaa
     6241 gagggaagcg tactctatgc cacaccaaat gccaatacat caaatagttt tagacccgac
     6301 tttctttatg tggtacatag agatgataat atttcgattg ttgctcaaag caaggcaggt
     6361 gtgggattgc tttatcaagg gctgacaatt cggggaattg ttatgattgc gataatggtt
     6421 gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc gatcaaagcc
     6481 ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc gctggagcga
     6541 aaggatgagc ttggcgcact ggctcatgat atgcattcca tgtatgtcag gctgaaagaa
     6601 accatcgcaa ggctggagga tgaaatcgca agggaacatg agttggagga aacacagcga
     6661 tatttctttg cggcagcttc tcatgagcta aaaacgccca tcgcggctac aagcgttctg
     6721 ttggagggaa tgcttgaaaa tatcggtgac tacaaagatc attctaagta tctgcgcgaa
     6781 tgcatcaaaa tgatggatag gcagggcaaa atcatttccg aaatactgga gcttgtcagc
     6841 ctgaatgatg ggagaatcgt acccatagct gaaccgttgg acatagggcg cacggttgcc
     6901 gagttgctgc ccgattttca aaccttggca gaggcaaaca accagcggtt cgtcacagat
     6961 attccagccg ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc actatccaat
     7021 gtcatattga atgcagttca gaacacgccg cagggaggcg aggtacggat atggagtgag
     7081 cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat tgatgatact
     7141 gcgcttccaa ggctgttcac cccattctat cgcattgatc aggcgcgaag cagaaaaagt
     7201 gggcgaagcg gtttaggact tgccatcgta caaaaaacgc tggatgccat gagcctccag
     7261 tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc tggatttacc gctcacatca
     7321 acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc tgccggtctt
     7381 tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat gcgcttgcca
     7441 taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca ttcgatcaaa
     7501 tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg aaacaatccg
     7561 tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt
     7621 tggggtggag actggcttcc gtattggagg caacacagat accgcccatc cctgcaactc
     7681 atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt gccaccgcca
     7741 aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag aaccccatcc
     7801 ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata gatattcgga
     7861 tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt tacccgattg
     7921 tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa aaaattgccg
     7981 aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa acttgggtgg
     8041 ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat gcggacggaa
     8101 ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat cgttttggtt
     8161 ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac gagccgtggc
     8221 attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg gtttgccttg
     8281 aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca gaaaaagatt
     8341 gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa tgtgctttta
     8401 tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa aatcgctttc
     8461 ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat ttgacatgat
     8521 ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa acggcttatt
     8581 gataagaccg ggggaaacct tttctttctg gtggctggta cgccatgcgg acaaagatac
     8641 cccctataaa gacggcctta cggtgaccaa tggtaaactc accaccatgt cgggcggcgg
     8701 tatgtaccag atgagcaatt tactattttg gatgttcctg catacgccat tgacaattat
     8761 ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg agattaaagg
     8821 ggtggatgca accatttcag agggctggat tgatttaaaa gtgcgaaacg ataccgactg
     8881 cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg tgtccgccga
     8941 caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg tccgtgaaag
     9001 tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag gtaccgggga
     9061 aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc ccctcccgga
     9121 aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac tgtttttgga
     9181 tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca tattatccct
     9241 acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg caatcaatgc
     9301 gttagcgtag gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc
     9361 ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac gactgccgcc
     9421 gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt tgcggattat
     9481 gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg
     9541 caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat gactgtggga
     9601 gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc
     9661 catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca gcttgatgag
     9721 cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga tacccgccat
     9781 ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat caacaccggg
     9841 cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt
     9901 ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga ttgctcgcag
     9961 aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt gatcatcaca
    10021 ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg
    10081 aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg caatcatctt
    10141 cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa ttgctgcgaa
    10201 cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg gtgtatggaa
    10261 gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca tactctcccc
    10321 ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat
    10381 tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga tacaggggct
    10441 gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg cagcttgcat
    10501 ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca
    10561 aatgattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg tctttgtgaa
    10621 gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg aagaacttaa
    10681 cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc aagcgatttc
    10741 gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg tcggcgaagt
    10801 ggatcaaatc cggctgagcc acggtatctt ccgcatccat caggaaaacg agccggaaaa
    10861 aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg
    10921 ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt
    10981 tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata ccatgcccgg
    11041 ttttacatcg tacagccgct acccacgtat ggtggccgcc gcaggaatca cgcttcctgc
    11101 actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga aaatggtttt
    11161 ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc cacatgggat
    11221 aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc
    11281 gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta tggcttgctt
    11341 ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa
    11401 ccggaggaca agctcacaaa agaaaaattt taccccaata tagagcgagc cgagttgatt
    11461 acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat tgatcttacg
    11521 ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga ttttatggac
    11581 gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa tcgaagatgc
    11641 ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg gtggcactat
    11701 aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc ataatgaaag
    11761 tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg gacaatcttt
    11821 tactggaaag tggttaaagc atcttttgta atacattgca aattatagta ttcaatctgc
    11881 ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc cattttgtat
    11941 tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag caaaaggtgt
    12001 ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct aatccacttc
    12061 caccactcaa aaaaagcctt gcccctccac agggctattc cggttatgag tatgtactat
    12121 accatgtaaa tcagcagcat agcaaagcat tttctcgccc ggtaggtttg cgacctgccg
    12181 ggcgattttt gtcgtctttt gcggcccggc tgtccccaaa aaaatttttt gagaaatttt
    12241 caaaaaggag gtatttagcg ggtagctggc ggccttgtca aagtcttgtt agcggaaagg
    12301 gagagaacca cctaagtccc ccacagaaag ggggtgagaa gatggatgct atcccccgcg
    12361 actatgaagc gcggtgcatg ttcgatgctt tttgcaagac ggtactgcgt aacgaggcca
    12421 aaagctactt ggctgaaatg aagcgccgcc gtgaccgtga agtgtcacta agctctttgt
    12481 cacaggcaga tttggacaag ctctgtacag tagatcacta ccccagcgac agctttacgt
    12541 tttcgtcgca tggctatgac ctacatatca acaatgagct tgttgccgaa gcctttgccg
    12601 cgctgcccag catggagcag agtattttaa tcctgcactg tgttctggat atggcagatg
    12661 gtgagatcgg cggccttgtg ggaatgtccc ggagtgccgt tcagcggcac aggacaaata
    12721 ccttatcgga gctgcggaaa cagctaaagg cgttgatgcc gaagggaggt tagacgcgga
    12781 tgaagaaccc gaactacaag ggcattgagc ttttgcccct ttcggtgatc gaggccgccc
    12841 gcgctgggga tgccggagcc gtagagcgcg ttttgcagta ttacagcggt tacatgaaca
    12901 agctgtgtac ccggacgctc tatgacgaaa acggctatcc gcatgtgtgt ctggacgagg
    12961 ccatgaagcg ccgtctggaa atcaagctca tccacgctat cgtcacgaac tgacgaaccg
    13021 gccccggcag catgaaacgg cttacccttt ccccgtttcg gtgttttccc ggtggcctga
    13081 tcgcaccttg ataaatatgc ccgcaagata acggcggcgc atcatagggc atacggacac
    13141 attctgtctg tgccgagccg gacggctggt gcgccatgat acttttccct acgggaaaag
    13201 cggagcgagg aacaccacgc cgcaatgtga gcgtggcggc ttgcgggcga cgacgtacag
    13261 gcaggactat gatactccct tatagccaca gtccgagcgt taaaagcgtc gcaggcaatg
    13321 ggtagggccg cgtgagaacc acgcgggggt gaaagtcccg tggagctgtg ccagcagccg
    13381 tccgtctatg cccctaaatc acacaaacct ttgaatttgt gaaagggggc tgcattatga
    13441 accatgatgt gcctatttgg gaaaaataca cgctgacgat tgaggaagcg tctaaatatt
    13501 tccgtatcgg agaaaacaaa ttgcgtaagc tggccgagga aaatccggcc tctggctggg
    13561 tcattatgaa tggcaaccgc atccagatca aacgcaagca gtttgagaaa ataattgaca
    13621 ctttggacac aatctaatag tgaaaaaggg ccgcagatat gttataataa tttataagca
    13681 tatatgcggc tctttccgtc atggaaagga gcatgacgag atgtcagaaa aaagacgaga
    13741 cagcaaaaat cgcgttttgc ggtctggaga gagccagaga aaagacggac gatatgctta
    13801 caaatatgtt gatacctttg gaaaaccgca gtttgtttac tcatggaagt tagtgcctac
    13861 ggataagacc ccggcaggta aacgggagga tatttcactc cgggaaaagg agaaagaaat
    13921 ccagaaagac cttgacgacg ggatagacac catcggcaag aaaatgaccg tctgccagct
    13981 ctacgccaag cagatcaggc accggggcaa tgtgaagcac aacaccaaac tgggccggga
    14041 acggctgatg cgtattctgg aacaagatcg gctggggagc tgcccgattg acagtgtgaa
    14101 gctgtccgac gcaaaggaat gggcgcttcg gatgaaagaa aagggccttt cttataagac
    14161 catcaataac gacaagcgtt ccctgaaagc ggctttttac accgccatac aggacgattg
    14221 catccgaaaa aacccgtttg acttccagct tagtgacgtt ctggacgacg atacggaacc
    14281 aaaggtgcct cttacaccag cacaggaaga aagttttctg tcctttattc aaggtgataa
    14341 ggtttaccaa aagcattatg acgcaatcgt tattctgctg gggacagggc ttcgcatttc
    14401 ggaactgtgc ggactgacgg acaaagattt ggattttgaa aaccgggtaa tcatcgtgag
    14461 ccaccagctt ttgagaaata ccggcgtcgg ttactatatt gacgaaccca agacccaaag
    14521 cggcgtcaga aaaatcccca tgaatgagga agtgtatcag gcgttccagc gagtgataaa
    14581 aaaccgcaaa ggtgcaaagc cgttcattat tgacgggtat gccaatttcc tgtttctcaa
    14641 acagaacggc tatcccatga cggcggtaga ttatggcggg atgtttggca ggcttgtgaa
    14701 gaagtacaac aagagccatg aagaagcgtt gccgaaaacg acgacgcccc atgctatgcg
    14761 tcatacattc tgcacccggt tagccaatgc gggaatgaac cccaaagcct tgcagtacat
    14821 tatgggacat tccaatatca ccatgacgct gaactattat gcacacgcca cattcgattc
    14881 cgcaagggca gaaatggaac ggctggcagc ctgacacaag gccggtttta ctactccatt
    14941 tactactttt gagggggaaa tggtgagagg taataagaac atttgtaagt atctaacaat
    15001 atcaaaaatg ccggaaaagc ccggaaacac gggctatacc gacattcaag aacttctaaa
    15061 aagataatct aaaatcccat ataattttca atgaaaagta gtttcagact atttaaataa
    15121 cgaaggcggc tac
//
LOCUS       DAFGFZ010000371        13612 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.078, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000371 DAFGFZ010000000
VERSION     DAFGFZ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.078"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..308)
                     /locus_tag="LR726_003241"
     CDS             complement(<1..308)
                     /locus_tag="LR726_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218227.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     G"
     gene            820..1455
                     /locus_tag="LR726_003242"
     CDS             820..1455
                     /locus_tag="LR726_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218228.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1609..1686
                     /locus_tag="LR726_003243"
                     /pseudo
     CDS             1609..1686
                     /locus_tag="LR726_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1931..2047
                     /locus_tag="LR726_003244"
     CDS             1931..2047
                     /locus_tag="LR726_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL6218229.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2063..3982
                     /gene="tet(M)"
                     /locus_tag="LR726_003245"
     CDS             2063..3982
                     /gene="tet(M)"
                     /locus_tag="LR726_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL6218230.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4101..4268
                     /locus_tag="LR726_003246"
     CDS             4101..4268
                     /locus_tag="LR726_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL6218231.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(4328..4681)
                     /locus_tag="LR726_003247"
     CDS             complement(4328..4681)
                     /locus_tag="LR726_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218232.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            5185..5604
                     /locus_tag="LR726_003248"
     CDS             5185..5604
                     /locus_tag="LR726_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL6218233.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            5630..5836
                     /locus_tag="LR726_003249"
     CDS             5630..5836
                     /locus_tag="LR726_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218234.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(6064..6237)
                     /locus_tag="LR726_003250"
     CDS             complement(6064..6237)
                     /locus_tag="LR726_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL6218235.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            6213..6878
                     /locus_tag="LR726_003251"
     CDS             6213..6878
                     /locus_tag="LR726_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL6218236.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(6960..8126)
                     /gene="dnaJ"
                     /locus_tag="LR726_003252"
     CDS             complement(6960..8126)
                     /gene="dnaJ"
                     /locus_tag="LR726_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL6218237.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8277..10106)
                     /gene="dnaK"
                     /locus_tag="LR726_003253"
     CDS             complement(8277..10106)
                     /gene="dnaK"
                     /locus_tag="LR726_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL6218238.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(10157..10720)
                     /gene="grpE"
                     /locus_tag="LR726_003254"
     CDS             complement(10157..10720)
                     /gene="grpE"
                     /locus_tag="LR726_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL6218239.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(10814..11857)
                     /gene="hrcA"
                     /locus_tag="LR726_003255"
     CDS             complement(10814..11857)
                     /gene="hrcA"
                     /locus_tag="LR726_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL6218240.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(12091..13256)
                     /locus_tag="LR726_003256"
                     /pseudo
     CDS             complement(12091..13256)
                     /locus_tag="LR726_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(13272..13499)
                     /locus_tag="LR726_003257"
     CDS             complement(13272..13499)
                     /locus_tag="LR726_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL6218241.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
BASE COUNT         4047 a         2639 c         2494 g         4432 t
ORIGIN      
        1 ccatagtcat aatggataaa ataatctgct ttcatgccaa gcacctttcg gataatttcc
       61 aatgcgtcag tcgttggata acggacagac agataatcaa cgagcagata aaaaggctta
      121 tcgcagatat aataattcag acaacgcagg ataatatttt gtgtttcctt gctggcgttg
      181 gcttttccgt tctcaaagcg gttgatacta tatcttgata catgggtaag acttgcaagt
      241 tctccttgtg aaagaccgct tgcaagacgt ttgttcttca tttcaagtgc aaattctaaa
      301 ttgttcattg tattcctcca atcccacaat agccttgaaa tacctgtaaa cctctattca
      361 aaaagtgtga tatgcactat cttcttaaaa cttctcatta ctactcacaa ctttgggatt
      421 gtatggtttt tgacttgtct tttgtgaaat agtgcccccc tgttagataa cgggggcaca
      481 gacaaggctc gcaggctcgc cagccgacaa agcatttctt ccccttgaca caaggtgtca
      541 tggcggtaag cctgagaggc ttccgccacc gtgtcagaaa tactttgtca gcaagttctg
      601 ttttgccttt tctaacaagt tccatttcaa ccgatattca tatctacgga tttaatcaga
      661 taggtatttc tttttgaatg tttttatatt tcattctatt gtgcttattc gattacaaga
      721 acatagtaat tcttatatgc ttatttgtca atatcttttt ttgcctttac aaatcatatt
      781 aagcatgata gacttataat agcgaagata taacgagata tggagggtta tgcaatgaaa
      841 ataagccatt taggaaataa tatacagacc ataagaaaat ttaggggaat gaaacaacag
      901 gaacttgcgg acaaaatcgg tatcaatatg cagagccttt ccaagattga aagagggttg
      961 aactatcctg cctatgaaac gctggaaaag ataatggaag tgctggacgt tacgcccaat
     1021 gaattgttgt cgggagaatg gaagtatgtc aatcaatctg aaaaggaagt ctgtcagttt
     1081 ttaagagccg aagaacgtct aaatgcagaa ctgaaacatg gacactatga taacttcttt
     1141 gacagcgagg aagaatggct ggaatatgag ttagaaaagt tacgggaata tattacagac
     1201 tatatcaacg ggaaaagcat tgaagcgtcc gacctttacc caatcaaaga atttatccag
     1261 catttgaagt ttcaaaaatt attagaccgc tatgatgatt tatacagtat ggatatgttt
     1321 ggggaaagca tagagggaca taagtataag acaccttatc aagtcgtgaa aatgataaac
     1381 ccgaactcaa aagaagatat ggaactgtta cgagaagtat taaggaataa caattttgat
     1441 gatgaggacg agtaactttt ttcctcttgc gagaagatac agcaaacaaa aagcccgatc
     1501 aagagtgcaa agcaccacca atggtggcaa agctcttgac agggcttttt cttttctgtt
     1561 gtgggcaatc aagaggaaga aagggataaa atctgatttg tggtaaatca ggcaaaggca
     1621 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
     1681 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
     1741 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
     1801 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
     1861 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgtacccag tttaagaata
     1921 cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta tgcctatggt
     1981 tatgcataaa aatcccagtg ataaaagtat ttatcactgg gatttttatg cccttttggg
     2041 tttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
     2101 tgcaggaaaa actaccttaa cagaaagctt attatataac agtggagcga ttacagaatt
     2161 aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac gtcagagagg
     2221 aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg tgaacatcat
     2281 agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat cagttttaga
     2341 tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt
     2401 tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga ttgaccaaaa
     2461 tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttctg ccgaaattgt
     2521 aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga
     2581 acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata tgtccggtaa
     2641 atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga attgttctct
     2701 gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc ttatagaagt
     2761 tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt gcggaaatgt
     2821 tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tatatacgcc tttatagtgg
     2881 agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga
     2941 aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat
     3001 tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt
     3061 gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag
     3121 taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc
     3181 gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa
     3241 agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact
     3301 aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca
     3361 catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc
     3421 gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcgtt
     3481 tcaaaatgca gttatggagg ggatacgcta tggctgtgaa caaggattgt atggttggaa
     3541 tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg ttagtacccc
     3601 agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa aagctggaac
     3661 agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat atctttcacg
     3721 agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat tgaaaaataa
     3781 tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc gtagtgattt
     3841 aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt accatgttac
     3901 taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata aagtacgata
     3961 tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg tttcttgtta
     4021 aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag gagatagtta
     4081 ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa
     4141 gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga caagaaaatt
     4201 taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca aagacgcaga
     4261 gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta tgtatggatt
     4321 cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa gattccatta
     4381 aggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat cgtcgggtgc
     4441 tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga agtaacgata
     4501 caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata ttagttaagt
     4561 accgtgggtc aatttcaatc aatgctccca cttgttcacg agttaaacct cgtttcaatc
     4621 gagcttcttt aatggctaaa ccaaaggctc taaaatcata tttatcttct tttttacgca
     4681 tagtagacca cctctataca ttttattgtt cctactgaat taaaaacagg tatagaaaaa
     4741 cgtgttatat ggtttatagg tttatattta ataaaaagca ctactaaacg ccaataaaaa
     4801 aaaccgttat atggtagtgc tatttacgct gttaaaatat tgtatattac ttccaaatgg
     4861 cggtttgttg gaggtcaacg tcgccatgaa gtacatcata tacaataaat ttccttacat
     4921 tgggttcttg tcaaaaaaag tcgtctatct gcaatagata agtacgtcca ccaatgtggt
     4981 tttataaatc atatagatag aataacagaa gcatgtaaac agagaaataa atctgtttat
     5041 atgctttttt ggctattcag aactttttta caaagtttat ttatcagtaa tgcaacaaat
     5101 ccccctttca cattgggact aagagtgaaa ggagataaac gagcaaggct cacttccttt
     5161 cctagacaga aagggggtga gattatgagg acatcttctt tcgagcatat cgtcagatta
     5221 cagttcaatt ctttaatgct gattgtcatt aagaacaaag tgaaaagtcg aaacagacag
     5281 cttgcaagac attctaaacg tgaagtattg ttttgtgaaa tagccgaaac gaaacaagtt
     5341 gaacgtggaa cgattgatac ctgtttttat gattgtgtat catttaaagt cttgcacttt
     5401 acaatttatg tatctgatga aacacttggt acagttttac atagactatc agaaaaacag
     5461 cgtagtgcga ttttattacg ttattttcaa ggaatgaacg acagagaaat ttcagaactg
     5521 taccatgtat cacgttctgc tatttgcagt agaagaacta gaggactaag gaagttaaaa
     5581 atgcttttga gcgaaaggaa gtaaagatta tggctatccc tcttttgaat tgatttgcaa
     5641 agcgtcaagc ggagatgaaa tggcagttaa agagattttg aagttttatg acggttatat
     5701 ttctaaatta tgcttgcgtc ctttttacca ctctgaaagt ggtaaaatca ttatgcaggt
     5761 tgatgaagaa ctaaaaggag aaattcatac agacatgatg aaagcaatat tgaaatttga
     5821 aatcggagtg aagtaatcaa ctgtataaaa aaatcgggag atacacagtt tcgtgttatc
     5881 tcccttagta gttttacggt catatcaagg ctcattcaat cgtggtaaat tcgtggtaag
     5941 atgagtgttt ttctatactt caaaatgctt gaatgtatag tgtttatgcg gataattgaa
     6001 aattagatat aaaatttaat aaaaaccaaa aatgcacggt cgccatgaag ggaccgtgta
     6061 tttttatctt cttattttct cacctaatcg tatcagccgc atcgcttgtt ttcctgcaca
     6121 aacaagcaat cgctctacct gttccatcgc ataagaaagt accatcggtt cattgatgat
     6181 atcaagtaca tagtcgatac caccctcact taattgagct tgatttttct tctccatatt
     6241 atgtagttgt gggacaactt tctgacaatc agcaattgtg tcaagaacgg atcaatcctt
     6301 tattggagtt gctcgaaagg gtcttctctt attacggaca ggctttgctc actgtacata
     6361 gacaacaaat cattatccta gtcaatcgga tttccagagc ctcattgcta tcattgcttg
     6421 ataaaatcca gacaaagttt aataaaatgt atcagttaca gctaaacttt ggaattgggt
     6481 cgctttgtta tacagagcaa gagacaccgc aatctttcct tcatgccaaa caagtttgcg
     6541 aatggatagc tttccatcaa tctgtgaacg agatccgctt ttttgaagat ttagacctcg
     6601 ggatcgtttt accagcaatc ccgtcagatc aacgaacatt atatgtaaaa cgtattttga
     6661 aaagcctgac agaagaagaa gtacatttgt tcaagaagac tttggcatgc ttcagtaaaa
     6721 ataacggaag catcaaaaat tgctcagaag aattatttat tcataaaaac accttgcaat
     6781 atcgattgaa caagttccat tctctcactg gttatacccc tagaaactat gacgattatc
     6841 atattctaaa gctggctttt ttgttggtac aaacatgaca actacaaaaa aaccaggcga
     6901 acatatgatt gttcgtctgg tttttaatta ttaatgactt agcgttttta ctagaaaaac
     6961 tacttccgct ttttcccgcc aaaagcatct ttaaacttat cgaagaaacc gtcttcttgc
     7021 tgttcttcaa ctttgatgcc gccagcttca gcgaatgcac gtaatgcttc tctttgttgt
     7081 tcatttagat ttttcggtgt gatcagtttt actttgacat gttggtcgcc tgttgcacca
     7141 ccacgtaact tcggagcgcc ttttccacgt aaacggaagt tcgtacctgt ctgtgttcca
     7201 gcagggatct tcaatttcac atctccatgg acagttggta cttggacttc atcccccaaa
     7261 gctgcttgta caaaacttaa aggcaattca tagtaaattt cagaaccttc acgatcaaag
     7321 atatcacttt cttctacacg gaagaccaca tacaaatcac caaatggtcc gccgttttct
     7381 ccagcttctc cttgaccagc taaacgcatt tgttgtcctt cttctacacc agctggtaca
     7441 ttcactttaa ctgaatgtgc tttcttctca tgtcctgagc catgacaggt tggacaaggt
     7501 tctttgattt ctttccctgt cccatgacaa acatcacaag tttgacggct catcactcgt
     7561 ccaagtggtg tttggcgttc tacattaatc gtacctgcac catgacattt atgacaagta
     7621 gttggttgtg tgcctggttt tgcaccgttc ccaccacaag tatgacagat ttcttcccga
     7681 ttatacttga tttctttttc tacaccaaaa atcgcttctt caaaagacaa gtcgaccgta
     7741 tattgcaaat cagcaccttg acgaggcgca tttggatcga ctgaccgacc gcctcctcca
     7801 aagaaagagt cgaagatatc ttcaaaacca ccgaagccgc caccagagaa gccgccgaat
     7861 ccaccaaatc cgccaccagc tccaccagca ccgtaatttg gatctgtgcc tgcatgcccg
     7921 tattggtcat atgcagcgcg tttttgcggg tcacttaaaa tctcatatgc ttctgatact
     7981 tctttgaatt tttcttccgc atcaggttct ttattgatgt caggatggta ttttttcgaa
     8041 agcttacgat aagctttctt tatttcatct tctgatgctc cttttgagag ccctaagact
     8101 tcataataat cacgttttgt tgccataggc tttcctccta ttggttctca tcattttcac
     8161 gtcgcttgtt atcatatcat attttgtttg aaacaaaaac cgatacttca aactattttt
     8221 caaaagaaaa agccaaagta gcaactttgg ccttctctcg aaattcagtg aactgattat
     8281 ttatcgtcgc catcgacttc ttcaaaatca gcatcaacga catcatctgc tccgccttga
     8341 gctgcttctg ggttttcttg agcttgttgt tgtgccgctt gttcgtatag tttaacagtc
     8401 aagttttgaa cgatttcgtt caatgcatcg cgtttagttt tcatttcttc gatgttgttt
     8461 gcttcaacag cggctttcaa ttcatcgcga gctgtttctg cttttttcac ttcttcttcg
     8521 tccactttgc cttctaattc tttcaatgtt ttgtccacag agaatagcaa tgcatctaca
     8581 tcattacgta ggtcaacttc ctctttacga gctttatctg cttctgcatt tgcttctgca
     8641 tcttttacca tacgttcgat ttcttcgtct gttagacctg aagaagattt gatcgtgatt
     8701 ttttgttctt tttgtgtacc tagatctttc gcagatacat ttacaatccc gtttttatca
     8761 atatcgaatg ttacttcgat ttgtggaata ccacgaggag ctgctggaat atctgtcaat
     8821 tggaatcttc ctagagtttt gttgtcagca gccattgggc gttcaccttg taatacatgg
     8881 atgtctactg ctggttgatt atctgccgca gttgagaata cttgtgattt acttgttgga
     8941 atcgttgtat tgcggtcaat caatttagtg aacactccac ccattgtttc gatacctaat
     9001 gacaatggtg taacgtcaag taaaacaaca tctttcacat caccagtgat cacgccacct
     9061 tggatagcag cacccatagc aactacttca tcagggttaa ctgatttgtt tggttctttt
     9121 cctgtttctt tacgtactgc ttctacaact gcagggatac gagtagatcc accgactaaa
     9181 ataacttcgt cgatttctga ttgtgataaa cctgcatctt ttagcgcttg acgtactggt
     9241 acttttgtac gttctactaa gtcagctgtt aattcatcaa attttgcacg tgtcaatgtc
     9301 atttccaaat gcaatggtcc agattctcct gctgtgataa atggcaagct gatttgtgtg
     9361 cttgatacgc cagataaatc ttttttcgct ttttcagcag cgtctttcaa gcgttgtaaa
     9421 gccattttgt cttgagacaa gtcaatgctg ttttcttttt tgaattctgc taccatgtag
     9481 tcgatgattt tgttatcaaa gtcgtcccca cctagatggt tgtcgccagc tgttgacaat
     9541 acatcaaaga caccgtctcc tagttcaagg atagatacgt caaaagtacc gccaccaagg
     9601 tcgaatacta agattttttc gtcgcgatct gttttatcta aaccataggc aagagcagct
     9661 gcagttggtt cattgacgat acgttctact tctagaccag cgatcttacc agcatctttt
     9721 gttgcttgac gttgtgcatc attaaagtaa gctggcacag tgataactgc tttttcgact
     9781 ttttcaccta aatagtcttc tgcgaatcct ttgatatatt gaaggatcat tgctgagatt
     9841 tcttgtggtg tatatgattt accttctaca tctactttgt aacctgcttc accgatatgg
     9901 cgtttgattg atgaaatcgt atttgggttt gtcacagctt gacgttttgc aacttcccct
     9961 acttggattt ctccattttt gaatgataca acagaaggtg ttgtacggtt tccttctggg
    10021 ttagcgatga tttttgcttc gtttccttcc aatactgcta ctgcagagtt agtcgtacct
    10081 aagtcaatac cgataatttt actcataatt aacgtctcct tctttaattg aaagtgtaag
    10141 ttttatatcc gcacaattat tgtgcaacaa tgaccatggt tggtcttaac acgcgatcat
    10201 gtaatttgta gccttcttgt agtacttcta caatcgtatc ggctggtgta tcttcagtcg
    10261 ctggaacagt ttgtactgct tgatgaagat taggatcaaa ggcttctcct ttagcaggga
    10321 ttttttcaat tccttcttct tccagtgcgt tacgcaaact ttccaagacc atttccacgc
    10381 cttttttcaa acctgcccct tgatcatctg acacttctgt ggctaatgca cgttccaggt
    10441 tgtcaattga tggaagtaat tttttggcta aatcttgtga acggtatttt tgtaattgct
    10501 cacgctcatt ttttcctcga tttgccatat ttgcgatttc agcacgagcc cgcaagtatt
    10561 tatcttccat ttcttctaac tcagctttta atttcgctgt ttcgaattct tccgcttcag
    10621 cttctgaaat acccgaatct tctgcagctt cgacatcgat ttccggttct ggctgagcat
    10681 ctgtcatttc tttatctagt tcttcttgat tttctttcac gatacagtct tcctttcatt
    10741 attcagtcag ctgtttgatt gctcttacta agaaagagag aacgatcagc tgcaaatagc
    10801 ttcgttattc acattacgag tttgacaagt caagcgaacg gtagtaatca gcaagtttag
    10861 ctgctagttc tcttcgataa acatcgacta gaccgaacat ccgagaataa ggcatactag
    10921 taggccctaa caaggctatt gtacctcttc catgaccatc tatctcgtaa cttgcttgaa
    10981 tcatactcat attctgcaat aaatcattgc ctaattcagc accaatcttg atatgaatat
    11041 tgctatccat cggagtgatc atttgtgtaa gtccgtctga gtctttcata aaagaaaaca
    11101 tcgatttgaa ctgatcaaga tcttgacggg gctcataatc caagagattc atttgtccac
    11161 tgacataaac cttatcttca aaaacatgac caagaacggt gtcaaataac tgagacatcc
    11221 cttctgtcgt ttggaaatat ttatgaagaa tcatcggaat ctccgtacga agtttatggt
    11281 acaccgtcat cagaggatct ccgactagac ggtcgtttac aatctgtacc attttttcta
    11341 gttcctgact tcccatatat tcgggaagag tgaacacttg gctttctaca ttccccatat
    11401 ctgttacgac gatagcaata atctgtcgat tattcaaggg tacgatacga aagccagtca
    11461 gtcggcggtc ttttatttca ggtcccaatg aaaaagtcgt atagcttgtc agctcagata
    11521 atatctcagc agattgctta ataatctcat tgatttcatg aaattctctg cctagagact
    11581 gacgaatcag cagcaaatca tcttctgcta cgcgagcagg ttttaacaaa tgatccacgt
    11641 aataacgata gcctaaagca gatggaatcc gcccagaaga agagtgagtc ttcaacagca
    11701 atccttcatc ttctaatgct ttcatatcat tgcgaatcgt tgctgagctg gccttgactc
    11761 cctcagacat caatgttttg gaaccaacgg gcacgccact actcgtatag tgttggatga
    11821 tcagacgtaa aatgtcgttt tgtcgctttg ttaacatctt cctcacctct cattagcact
    11881 catatattct aagtgctaat ccacttataa atatagcaag aagcccttct cctgtcaaga
    11941 gaaagactcc attttttagc actctaaaca caagagtgct aattctaaat aattgatgta
    12001 aatccagtta tatcaacgtt tcaatagtcc tttattgtat ttttgaactt tttttattta
    12061 aattcagata aaaaacaaat attctttaaa ctatttttca aacaaaaagc gctcaaacac
    12121 attgttcccc aaaaaggtcc ctcttggtgt caaataaaca cggtcttctt cattaacaag
    12181 taacccttct tgctctaatt cccttagcac atcaccgtac accgattcaa ttgttttttg
    12241 atagcgatca tagaaatgtt ttttagtaat ccctgttttt ttcttagtcc taagaacatt
    12301 tcttcttcta tttgattttt tcgtgtcaat ttttcttctt ccaataccgg gcgttcattt
    12361 gctcgtaatg ggcgtaaata gtgttggatt ggtcctttgt tgcggtaacg aatatctcca
    12421 agataaccgc tagcaccagc tccaaaccca aaatagtggt cattgttcca gtacatgagg
    12481 ttatgttgag attcatgtcc ggagagccca aagttactta tttcatattg gtgacgtcca
    12541 tgcttttcca ttgcttcaat cgtctcttca aacattctgg tttccgtttc ctgatcaggc
    12601 agttcgagtc ttccttgtcg tacccagttc atgaacattg ttttgttttc gaggatcaat
    12661 gagtacaaag aatagtgagg cagatccaaa gccaaagctt tttccaaagt atccagatat
    12721 ccttctagtg tttgtcccgg taatgcataa atcaaatcga tgcttacatt ggtaaaattt
    12781 tctgcttcta aaaattgcat agtttgatac acatcttctg cggtgtgttt acgtccaatt
    12841 tttttcaata agcgattatc aaatgtctgc actcccatgg ataaacgatt gacgccatag
    12901 tttttcatta cttgaagttt ttcacgggtc aaatcacctg gattcgcttc tacagtaaac
    12961 tcattaccat tttgaaaagg caggatctct ctagcacctg ataacagtcg atcaagttgt
    13021 ttagcagata aagaagttgg tgtcccgccc cctacgtaaa gtgtttctaa attcgctgtc
    13081 ggatgacttt ctagcattat tttcatttca ttcagcagca tttccacata ctcatctact
    13141 ggctgccctt ctaaaaaaac tttattaaaa tcacagtaat agcagatatg ctcgcaaaat
    13201 gggatatgaa tgtacgcaga aaccttgttt tctgcgggat ttattgtttc tatcattgaa
    13261 attctttctc cttacgtcga aaagtcgtgc ctgttttcga ccttcttaaa atcgcgttca
    13321 agcaccaact cattgaaata agccgcatat ttagaatcaa ggaaacctag ctctaaaaat
    13381 gcttgcttat tgtcatcgga gctacacggt gcttttacag cctctcgata aacggtaatc
    13441 gcaaagctga ccttcggcat taagtcaaaa tctgcaaaaa tatgaaaagc tattttcaca
    13501 gattcctcct taagtgctca gaccacaacg ctttgcttac aacctctcat atacgcatac
    13561 taaacgtatt tggcgaacaa atccactaaa aaatccaaga atcgacttct tg
//
LOCUS       DAFGFZ010000372         3939 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.176, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000372 DAFGFZ010000000
VERSION     DAFGFZ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.176"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            complement(<1..288)
                     /locus_tag="LR726_003258"
     CDS             complement(<1..288)
                     /locus_tag="LR726_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL6218242.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIIT"
     gene            414..1535
                     /locus_tag="LR726_003259"
     CDS             414..1535
                     /locus_tag="LR726_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL6218243.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1793..3295
                     /gene="eat(A)"
                     /locus_tag="LR726_003260"
     CDS             1793..3295
                     /gene="eat(A)"
                     /locus_tag="LR726_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL6218244.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3323..3889
                     /locus_tag="LR726_003261"
     CDS             3323..3889
                     /locus_tag="LR726_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL6218245.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1314 a          691 c          873 g         1061 t
ORIGIN      
        1 agtaataatg gattggatcg aacaaaacat atcatctaat tgattggctt ttgttaatgc
       61 tacgatcaag tccacgtgtt cgttgctttc gatgatttct tctggtgaat agattggcag
      121 ttttgcgacc acttctttac cgttaaagtc agcaatcaac ccatttgtac gaatggcttc
      181 aatgtgagca ggccaacgat cgatcaagag gacctcattt ccgctttgat gtagcatcaa
      241 tccgattcgg ctgcccattg ctccggcccc agctattgcg attttcatgt tttttctctc
      301 ctttattcaa tgagttccct tttagtatat agaaaaatgg aagaaaatag cattgttttg
      361 tttgaaaaaa atgaaaaaaa gtgtcttact taagaagaaa gtgagggata cagatggata
      421 ttttggcggc aattgattca tttaaaggaa gtgcttcttc atttgaacta aatcaggcag
      481 ttctaaatgg ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg
      541 gagaaaatac tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg
      601 ggatggatct cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa
      661 aaaacaccgc agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa
      721 cggatcaaac tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac
      781 tgcttcaaga agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg
      841 gtctaggatt actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt
      901 tattatctac taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat
      961 tgattatcgc tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat
     1021 ttggaagaca aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa
     1081 agattgctca gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg
     1141 gagcagcagg cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt
     1201 tcgatttagt cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca
     1261 ttacaggaga agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag
     1321 cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag
     1381 agctagaagg actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag
     1441 gtttagaaga agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga
     1501 cacttagcag atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct
     1561 atcgtaattt caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt
     1621 gatgaaagct gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg
     1681 ggattggctt ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct
     1741 ctttagccag ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa
     1801 aatcgaaata aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca
     1861 agcaaatcta aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg
     1921 aaagacaact ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca
     1981 tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta
     2041 cgtgttaaat gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat
     2101 gcaaacagat cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac
     2161 aaaagtccta ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc
     2221 aacgaatcat ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa
     2281 acaaggcttc atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt
     2341 tttagcaatc gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga
     2401 acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt
     2461 cagtaggcta aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga
     2521 taaaacaaag aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc
     2581 agtgggtgct gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga
     2641 gacccagatc agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac
     2701 gatgaatagc caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg
     2761 gtatgaaaat ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc
     2821 gatttcaggt cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt
     2881 caacggcaag gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag
     2941 ccaaaattat gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga
     3001 ctaccaagca tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa
     3061 caagatcgag cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc
     3121 acagccagct gaactatata catgggatga accattgaat tatttggatg tcttcaatca
     3181 agaacaatta gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga
     3241 tcaaaccttc ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt
     3301 gtaatctcaa gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca
     3361 catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg
     3421 acgggcagat gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa
     3481 ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag
     3541 agatacagca caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt
     3601 ggtagaaatg gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa
     3661 agcacgttta gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc
     3721 acggatcgtt cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt
     3781 ttgccataat gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg
     3841 gttctttaca ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg
     3901 tgttatttaa ataaaatatt tcaaggcact gcttttgct
//
LOCUS       DAFGFZ010000373         2993 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.195, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000373 DAFGFZ010000000
VERSION     DAFGFZ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.195"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..188
                     /locus_tag="LR726_003262"
     CDS             <1..188
                     /locus_tag="LR726_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL6218246.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="LR726_003263"
     CDS             203..1072
                     /locus_tag="LR726_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL6218247.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="LR726_003264"
     CDS             1053..1787
                     /locus_tag="LR726_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL6218248.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="LR726_003265"
     CDS             1820..2728
                     /locus_tag="LR726_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBL6218249.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2973
                     /locus_tag="LR726_003266"
     CDS             2725..2973
                     /locus_tag="LR726_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBL6218250.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1047 a          420 c          662 g          864 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga ata
//
LOCUS       DAFGFZ010000374         2460 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.207, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000374 DAFGFZ010000000
VERSION     DAFGFZ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.207"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            189..1403
                     /locus_tag="LR726_003267"
     CDS             189..1403
                     /locus_tag="LR726_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL6218251.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1434..1652
                     /locus_tag="LR726_003268"
                     /pseudo
     CDS             1434..1652
                     /locus_tag="LR726_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2071..>2460
                     /locus_tag="LR726_003269"
     CDS             2071..>2460
                     /locus_tag="LR726_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL6218252.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFD"
BASE COUNT          754 a          391 c          543 g          772 t
ORIGIN      
        1 gttttctata aaaagaagac cgtttatttt ttgtctttat ttgtgtgaaa aacgaaaaag
       61 agcacaacac atcaagcttt tattaattgt ctgtctttta taaatgcaag tattaattca
      121 aaaaaacaga agaaaaaaac aactacacct tcttttatac tcgtaatcgt agaaaggagg
      181 cgaaaaaaat gaatacatta cctatcaata caggagatac agcattcatg attttatgta
      241 cagcaatggt ttgcctgatg actcctggtc tagctttttt ttacggcgga ttagcacgga
      301 agaataatat actaacgatc atgtctcaat cttttctctc cgttgggata acaacgatta
      361 tgtgggtatt tggtggattc ggattggcct ttggacggga tatcggcggt gttattggaa
      421 acccggcaga tttctttttg atgagacatg ttacaagctt ccccaacatg tttcatggtg
      481 caacgattcc atttttaatg ttctttcttt accaactgat gtttatcgtg attaccgttc
      541 ctttgatgac aggagctttt gcagggagat tgaatttaaa aggctatatt atacttgtta
      601 ttttttggaa tctgctgatt tatttccctg tttgccactg gatatggggt ggcggatttt
      661 tggatcagat gggatttaga gatttcgctg gtggagcagt catccacacg acagctggat
      721 tcggctcttt agcatgtatc ctcacacttg gtcaaagaaa gattccttta ggagaaagaa
      781 gtcagcatag cagtcttatg gcagctgcta tcggtacagg actgctttgg tttggctggt
      841 tcggttttaa ttccggtggt gcacttcgag cagatgaaca agcagtcaat gcatttggtt
      901 caacattcat cgctcttgca tttgcgatga ttacttggct gatcattgca aaagtaaaag
      961 gaaatggctt tgattttgtc gacgtactaa caggatccgt tgctggattg gcagccatta
     1021 cgccttgtgc cggttatgta gaagcaaaaa gtgccatgct gattgggatc attgcgggta
     1081 tcgtctgtca tgctgctgtt gattttcgta aaaagaaaca atgggatgat gcacttgatg
     1141 tgtggggtgt tcatggcatg ggcggattca caggtacgat tctgataggg atctttgcat
     1201 ctaacagcca tttactaacg aatccaagca gctggtactt cttagggatt caagtgattg
     1261 gtgtcatcat tactgccgtg tatgcctatg tgctgacaac agtgatttta aaatcggcga
     1321 tgcactttac aacgatcact actacaaaag aagaacaaga agaaggattg gactttttct
     1381 tctttggcaa caaactcaat tgaaacaaat tcaaatagaa aagaggaaga ttcatgcaac
     1441 aaagcaaaac gaaaattcct gcaaaaaaat taacgttctt tggctttttg gctatgacca
     1501 tctcaatggt cgtttcatta tatgaatatc ccacattcgc gacctcaggc ttcagcttag
     1561 tatttttctt gttattaggc ggtttgctat ggtttatccc tgtggcattg tgcgcagcag
     1621 agatggcaac ggtgaaagga tgggaaaaag gtgtttccca aacaaaaaga tattaagaaa
     1681 cagcccatgt ttacctgctt gcaaacaaaa gaatattgct actagactaa gagatactac
     1741 ataagtgtgt tggtatctct tttttgttgc cgtgaaacag cagactattt catttctgct
     1801 tgattacctt aaaacttatt agtctatggt ttgtcaaggg cttgttgcgt gagaaatgct
     1861 gaatatcacc ctttacaaaa cataggctga taagtaactt ttcaaaagca gaaaagaaat
     1921 ggcaatgctt ataacaatat gatataatgc agatgaagac aaaaaaggaa tttgtcgagc
     1981 tggttagttc gagataattt tgagtttgtt aagatgtggt gcacttgatg gaatcacagg
     2041 tacgacatca tattatggag gatgaaatag atgataggtt tgattgttgc gaggtcaaag
     2101 aataatgtta taggcaagaa tggtaatata ccatggaaaa taaagggaga acaaaagcaa
     2161 tttagagagt taacaacggg taatgtggtt attatggggc gaaagtctta tgaagaaatc
     2221 ggtcatccgt tgcctaatag aatgaatatt gttgtttcca ccacaacaga gtatcaagga
     2281 gataatttag tttcagttaa atcattagaa gatgcattat tattggctaa aggacgagat
     2341 gtatacatat ctggtggata tggactattt aaggaagctt tgcaaatagt agataaaatg
     2401 tatatcacag aagtagattt aaatattgaa gatggagata cattctttcc agaatttgat
//
LOCUS       DAFGFZ010000375         2205 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.220, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000375 DAFGFZ010000000
VERSION     DAFGFZ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.220"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            45..848
                     /gene="lnu(B)"
                     /locus_tag="LR726_003270"
     CDS             45..848
                     /gene="lnu(B)"
                     /locus_tag="LR726_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HBL6218253.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            1536..2205
                     /locus_tag="LR726_003271"
                     /pseudo
     CDS             1536..2205
                     /locus_tag="LR726_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
BASE COUNT          749 a          346 c          454 g          656 t
ORIGIN      
        1 gttgccacaa taactaaaag atatttacat gaaagggtga agaaatgtta aaacaaaaag
       61 aattaattgc aaacgttaag aatcttactg agtcagatga acgaattaca gcttgtatga
      121 tgtatggatc gtttaccaaa ggagaaggtg accaatactc tgatatagag ttctatatat
      181 ttttgaaaga tagtataacc tcgaactttg attcatccaa ctggttgttt gacgtagctc
      241 cgtacttgat gctttataaa aatgagtacg gaacagaggt agttattttt gataatctta
      301 tacgtgggga atttcatttc ctttctgaaa aagatatgaa cataatcccc tcgtttaaag
      361 attcaggtta tattcctgat acgaaggcta tgcttattta cgatgaaaca gggcaattag
      421 aaaattattt atcagagata agtggtgcaa gaccaaatag acttactgaa gaaaatgcta
      481 attttttgtt gtgtaatttc tctaatctat ggttgatggg aatcaacgtt ctaaaaagag
      541 gagaatatgc tcgttcatta gaactcttat cacaacttca aaaaaataca ctacaactta
      601 tacgtatggc agaaaaaaat gctgataatt ggctaaacat gagtaaaaac cttgaaaaag
      661 aaattagcct tgaaaattat aaaaaatttg caaagaccac tgctcgatta gataaggtag
      721 aattatttga agcctataaa aattctttgc tattagttat ggatttgcaa agtcacctta
      781 ttgaacaata caacttaaaa gttacacatg acattttaga aagattgttg aattacatta
      841 gtgaatagga gagtaacgtt attcacagta ttgggtactg aaaattaaca gcatacctca
      901 gtttttcaaa aaacagcgaa gcctatggat atgttcccgt ctaccgatag tgcaaaaagt
      961 gtcgtggata tgtttccgag aacggacata cacaaatgac attcattctg tcctctcaat
     1021 ccatgtggaa gcagtatgtc tgttaacaag aaaataatta ttgttcgtcc tgacgagttg
     1081 ttagactcga aaaggataaa catcattttt agaaccggta gccgattttt ggctagcggt
     1141 tttttgggct ctttggcaaa tagtggtgat tgaatctcgc gctatcctat ggaaaaccgc
     1201 cttgtgtgag gggctgcgag accgcttcga tcctagaatt gctgaagcac gaatctagcg
     1261 tcactcacat ttcatctttt gacgtagctg agacaaggtg tttttcttcg atccgctagt
     1321 ggttaatctc tttttggtgc aaatatggtt tagaatttct ttgcatggct taagggagtg
     1381 gagaatcgga tattagtagg atttacatcc acagataaaa ataggcttga ttttaacagc
     1441 gaaactaaaa taccctactt aaatgaagtg atagattaga tgttgggttg ttaggagatg
     1501 cttaaaaatc ctatgcaatc aatagttttt agtgtttgtt gacttgatcc ctaaatcgcc
     1561 tgagtactaa acgttgagtg tgtctgctta ctgtcactag ccagtaagtg agactgtagt
     1621 taatctctca ttttccccag tattatgttg aaaatagcca tgaagcgatt atttctaaag
     1681 agttattttt gcaggctcaa gaagaaattt atcggagaag taatatctac acaggagaag
     1741 gcaagaacaa aagaatttat agtagcaagt acgctttaag tgccatcacc ttctgtggat
     1801 attgtggcga tatttatagg agaacctatt ggaatattca tggcagaaaa gaatttgtct
     1861 ggcgatgcgt gactagaatc gagcagggtc ccgaggtatg taagaaccga accgtaaaag
     1921 aagatgaact ctatggtgcg gtaatgaccg caattaataa gctacttgct ggtggcaaca
     1981 atatgataaa gacactggaa gaaaatattc atgctgtgat tggagaaacc acagaatacc
     2041 aaatttcaga gattaatacc ctactagatg aaaaacaaaa agaactaatc aagctggcca
     2101 ataaaggtca agattatgaa tacctggtag atgagatcga tgagatgcga gataagcgtc
     2161 agactctttt agtagaagat gcctccctca gtggcgagaa cgagc
//
LOCUS       DAFGFZ010000376         2026 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.229, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000376 DAFGFZ010000000
VERSION     DAFGFZ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.229"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            108..821
                     /locus_tag="LR726_003272"
     CDS             108..821
                     /locus_tag="LR726_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL6218254.1"
                     /translation="MQKNAKTLERRLQKIGETTKPQQMKQIRFPVPKSLELHSRYPIM
                     GQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGSGKTTLLEHIRKQGEGILLSPKVS
                     FQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSLLNHLGFAQETLTKPLCTLSGGEA
                     TRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEKLMQIYPGTILFTSHDSYFVERTA
                     DEVYEIKGQKIKKVLTRNF"
     gene            868..1314
                     /locus_tag="LR726_003273"
     CDS             868..1314
                     /locus_tag="LR726_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL6218255.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(1513..>2026)
                     /locus_tag="LR726_003274"
     CDS             complement(1513..>2026)
                     /locus_tag="LR726_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL6218256.1"
                     /translation="NTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKLD
                     QQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLDK
                     TTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQ
                     VREQMQQFFE"
BASE COUNT          609 a          355 c          416 g          646 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gaaaaagcaa caaatcagac
       61 tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg
      121 cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga
      181 aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg
      241 gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt
      301 tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat
      361 tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag
      421 tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga
      481 gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc
      541 aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga
      601 ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta
      661 ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt
      721 tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa
      781 aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc
      841 gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt
      901 acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat
      961 caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc
     1021 attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta
     1081 aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg
     1141 gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc
     1201 tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc
     1261 atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac
     1321 actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct
     1381 agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc
     1441 caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt
     1501 tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg
     1561 gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg
     1621 tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag
     1681 ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc
     1741 atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca
     1801 ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc
     1861 gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat
     1921 ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg
     1981 tggtttggat aaaagcacgc ataataatat ataggctaaa gtattc
//
LOCUS       DAFGFZ010000377         1349 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.275, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000377 DAFGFZ010000000
VERSION     DAFGFZ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.275"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            46..297
                     /locus_tag="LR726_003275"
                     /pseudo
     CDS             46..297
                     /locus_tag="LR726_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="LR726_003276"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="LR726_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL6218257.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DAFGFZ010000378         1154 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.298, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000378 DAFGFZ010000000
VERSION     DAFGFZ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.298"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            44..127
                     /locus_tag="LR726_003277"
     CDS             44..127
                     /locus_tag="LR726_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBL6218258.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            252..989
                     /gene="erm(B)"
                     /locus_tag="LR726_003278"
     CDS             252..989
                     /gene="erm(B)"
                     /locus_tag="LR726_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL6218259.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          440 a          187 c          176 g          351 t
ORIGIN      
        1 aattagatgc taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat
       61 gcgttatgta gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga
      121 taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat
      181 aactaaaatt acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca
      241 ggagtgataa catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag
      301 tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg
      361 gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta
      421 ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg
      481 tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa
      541 ttgttgggaa tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa
      601 gccgtgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata
      661 ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc
      721 tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc
      781 gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat
      841 gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac
      901 acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata
      961 gttatctatt atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag
     1021 ttacacgtta ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta
     1081 ccaattaatt cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc
     1141 ttatcgatac aaat
//
LOCUS       DAFGFZ010000379         1060 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.305, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000379 DAFGFZ010000000
VERSION     DAFGFZ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.305"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            344..892
                     /gene="aac(6')"
                     /locus_tag="LR726_003279"
     CDS             344..892
                     /gene="aac(6')"
                     /locus_tag="LR726_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL6218260.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          371 a          154 c          213 g          322 t
ORIGIN      
        1 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
       61 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
      121 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
      181 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
      241 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
      301 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
      361 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
      421 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
      481 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
      541 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
      601 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
      661 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
      721 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
      781 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
      841 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
      901 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt
      961 gaatttttgt gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca
     1021 gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
//
LOCUS       DAFGFZ010000380          648 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1700756
            SAMEA9565988-rid13454683.guided.345, whole genome shotgun sequence.
ACCESSION   DAFGFZ010000380 DAFGFZ010000000
VERSION     DAFGFZ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9565988
            Sequence Read Archive: ERR6615622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189603.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189603.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/04/2021 01:04:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,280
            CDSs (total)                      :: 3,218
            Genes (coding)                    :: 3,142
            CDSs (with protein)               :: 3,142
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 36 of 76
            Pseudo Genes (internal stop)      :: 21 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9565988-rid13454683.guided.345"
                     /isolate="DMG1700756"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2015"
     gene            <1..>648
                     /locus_tag="LR726_003280"
     CDS             <1..>648
                     /locus_tag="LR726_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HBL6218261.1"
                     /translation="SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTY"
BASE COUNT          271 a           45 c          113 g          219 t
ORIGIN      
        1 ttcgtatatt agtgatgaat tatctatact aggttataaa gaaattaaag gaactttttt
       61 aacaccagaa atttattcta ctatgtcaga agaagaacaa aatttgttaa aacgagatat
      121 tgccagtttt ttaagacaaa tgcacggttt agattataca gatattagtg aatgtactat
      181 tgataataaa caaaatgtat tagaagagta tatattgttg cgtgaaacta tttataatga
      241 tttaactgat atagaaaaag attatataga aagttttatg gaaagactaa atgcaacaac
      301 agtttttgag ggtaaaaagt gtttatgcca taatgatttt agttgtaatc atctattgtt
      361 agatggcaat aatagattaa ctggaataat tgattttgga gattctggaa ttatagatga
      421 atattgtgat tttatatact tacttgaaga tagtgaagaa gaaataggaa caaattttgg
      481 agaagatata ttaagaatgt atggaaatat agatattgag aaagcaaaag aatatcaaga
      541 tatagttgaa gaatattatc ctattgaaac tattgtttat ggaattaaaa atattaaaca
      601 ggaatttatc gaaaatggta gaaaagaaat ttataaaagg acttataa
//
